LOCUS       DACDTH010000001       113499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDTH010000001 DACDTH010000000
VERSION     DACDTH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 113499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 113499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..113499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.001"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1331
                     /locus_tag="IWK54_000001"
     CDS             <1..1331
                     /locus_tag="IWK54_000001"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9592156.1"
                     /translation="YAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLF
                     DDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGK
                     KAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYH
                     GQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSAN
                     WSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAE
                     GVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGK
                     NQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPID
                     MVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1321..2538
                     /locus_tag="IWK54_000002"
     CDS             1321..2538
                     /locus_tag="IWK54_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9592157.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2551..5700
                     /locus_tag="IWK54_000003"
     CDS             2551..5700
                     /locus_tag="IWK54_000003"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP9592158.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(7635..8300)
                     /locus_tag="IWK54_000004"
     CDS             complement(7635..8300)
                     /locus_tag="IWK54_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9592159.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            8426..10462
                     /locus_tag="IWK54_000005"
     CDS             8426..10462
                     /locus_tag="IWK54_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9592160.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            10466..11104
                     /locus_tag="IWK54_000006"
     CDS             10466..11104
                     /locus_tag="IWK54_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9592161.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            11132..11560
                     /locus_tag="IWK54_000007"
     CDS             11132..11560
                     /locus_tag="IWK54_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592162.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            12017..12928
                     /locus_tag="IWK54_000008"
     CDS             12017..12928
                     /locus_tag="IWK54_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9592163.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            13145..13582
                     /locus_tag="IWK54_000009"
     CDS             13145..13582
                     /locus_tag="IWK54_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592164.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            13677..14561
                     /locus_tag="IWK54_000010"
     CDS             13677..14561
                     /locus_tag="IWK54_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9592165.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            14785..17952
                     /locus_tag="IWK54_000011"
     CDS             14785..17952
                     /locus_tag="IWK54_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592166.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            17972..18517
                     /locus_tag="IWK54_000012"
     CDS             17972..18517
                     /locus_tag="IWK54_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP9592167.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            18545..19000
                     /locus_tag="IWK54_000013"
     CDS             18545..19000
                     /locus_tag="IWK54_000013"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP9592168.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            18997..19755
                     /locus_tag="IWK54_000014"
     CDS             18997..19755
                     /locus_tag="IWK54_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9592169.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(19841..20581)
                     /locus_tag="IWK54_000015"
     CDS             complement(19841..20581)
                     /locus_tag="IWK54_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9592170.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            20642..21895
                     /locus_tag="IWK54_000016"
     CDS             20642..21895
                     /locus_tag="IWK54_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9592171.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            22217..22444
                     /locus_tag="IWK54_000017"
     CDS             22217..22444
                     /locus_tag="IWK54_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9592172.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            22567..22893
                     /locus_tag="IWK54_000018"
     CDS             22567..22893
                     /locus_tag="IWK54_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9592173.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            22961..23674
                     /locus_tag="IWK54_000019"
     CDS             22961..23674
                     /locus_tag="IWK54_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9592174.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            23984..24835
                     /locus_tag="IWK54_000020"
     CDS             23984..24835
                     /locus_tag="IWK54_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9592175.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            24956..26821
                     /locus_tag="IWK54_000021"
     CDS             24956..26821
                     /locus_tag="IWK54_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9592176.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            26869..28278
                     /locus_tag="IWK54_000022"
     CDS             26869..28278
                     /locus_tag="IWK54_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP9592177.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            28386..29087
                     /locus_tag="IWK54_000023"
     CDS             28386..29087
                     /locus_tag="IWK54_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9592178.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            29246..29725
                     /locus_tag="IWK54_000024"
                     /pseudo
     CDS             29246..29725
                     /locus_tag="IWK54_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            30573..31148
                     /locus_tag="IWK54_000025"
     CDS             30573..31148
                     /locus_tag="IWK54_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9592179.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            31148..31588
                     /locus_tag="IWK54_000026"
     CDS             31148..31588
                     /locus_tag="IWK54_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9592180.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            31604..32305
                     /locus_tag="IWK54_000027"
     CDS             31604..32305
                     /locus_tag="IWK54_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9592181.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(32793..33218)
                     /locus_tag="IWK54_000028"
     CDS             complement(32793..33218)
                     /locus_tag="IWK54_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592182.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            33592..34563
                     /locus_tag="IWK54_000029"
     CDS             33592..34563
                     /locus_tag="IWK54_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9592183.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            34595..35992
                     /locus_tag="IWK54_000030"
     CDS             34595..35992
                     /locus_tag="IWK54_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9592184.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            36151..36774
                     /locus_tag="IWK54_000031"
     CDS             36151..36774
                     /locus_tag="IWK54_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592185.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            37523..39616
                     /locus_tag="IWK54_000032"
     CDS             37523..39616
                     /locus_tag="IWK54_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9592186.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            39776..40642
                     /locus_tag="IWK54_000033"
     CDS             39776..40642
                     /locus_tag="IWK54_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9592187.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40664..41101)
                     /gene="ndk"
                     /locus_tag="IWK54_000034"
     CDS             complement(40664..41101)
                     /gene="ndk"
                     /locus_tag="IWK54_000034"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9592188.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            41760..44438
                     /gene="mgtA"
                     /locus_tag="IWK54_000035"
     CDS             41760..44438
                     /gene="mgtA"
                     /locus_tag="IWK54_000035"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9592189.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            44525..45196
                     /locus_tag="IWK54_000036"
     CDS             44525..45196
                     /locus_tag="IWK54_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9592190.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            45843..46916
                     /locus_tag="IWK54_000037"
     CDS             45843..46916
                     /locus_tag="IWK54_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9592191.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            46918..47598
                     /locus_tag="IWK54_000038"
     CDS             46918..47598
                     /locus_tag="IWK54_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9592192.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTIYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            47615..48454
                     /locus_tag="IWK54_000039"
     CDS             47615..48454
                     /locus_tag="IWK54_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9592193.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            48485..49708
                     /locus_tag="IWK54_000040"
     CDS             48485..49708
                     /locus_tag="IWK54_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9592194.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            50111..51529
                     /gene="nhaC"
                     /locus_tag="IWK54_000041"
     CDS             50111..51529
                     /gene="nhaC"
                     /locus_tag="IWK54_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9592195.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(52033..52476)
                     /locus_tag="IWK54_000042"
     CDS             complement(52033..52476)
                     /locus_tag="IWK54_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9592196.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(52694..54034)
                     /locus_tag="IWK54_000043"
     CDS             complement(52694..54034)
                     /locus_tag="IWK54_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9592197.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            54303..55199
                     /locus_tag="IWK54_000044"
     CDS             54303..55199
                     /locus_tag="IWK54_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9592198.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(55349..56290)
                     /locus_tag="IWK54_000045"
     CDS             complement(55349..56290)
                     /locus_tag="IWK54_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9592199.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            56416..57537
                     /locus_tag="IWK54_000046"
     CDS             56416..57537
                     /locus_tag="IWK54_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9592200.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            57795..59297
                     /locus_tag="IWK54_000047"
     CDS             57795..59297
                     /locus_tag="IWK54_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9592201.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            59364..59891
                     /locus_tag="IWK54_000048"
     CDS             59364..59891
                     /locus_tag="IWK54_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9592202.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            60102..61163
                     /locus_tag="IWK54_000049"
     CDS             60102..61163
                     /locus_tag="IWK54_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9592203.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            61166..62062
                     /gene="murQ"
                     /locus_tag="IWK54_000050"
     CDS             61166..62062
                     /gene="murQ"
                     /locus_tag="IWK54_000050"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9592204.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            62076..64058
                     /locus_tag="IWK54_000051"
     CDS             62076..64058
                     /locus_tag="IWK54_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9592205.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(64123..64569)
                     /gene="nrdI"
                     /locus_tag="IWK54_000052"
     CDS             complement(64123..64569)
                     /gene="nrdI"
                     /locus_tag="IWK54_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9592206.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            64780..66204
                     /locus_tag="IWK54_000053"
     CDS             64780..66204
                     /locus_tag="IWK54_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9592207.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(66385..67158)
                     /locus_tag="IWK54_000054"
     CDS             complement(66385..67158)
                     /locus_tag="IWK54_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9592208.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(67155..68060)
                     /locus_tag="IWK54_000055"
     CDS             complement(67155..68060)
                     /locus_tag="IWK54_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9592209.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(68214..68534)
                     /locus_tag="IWK54_000056"
     CDS             complement(68214..68534)
                     /locus_tag="IWK54_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9592210.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            69008..69694
                     /locus_tag="IWK54_000057"
     CDS             69008..69694
                     /locus_tag="IWK54_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9592211.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            69696..70640
                     /locus_tag="IWK54_000058"
     CDS             69696..70640
                     /locus_tag="IWK54_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9592212.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            70839..71483
                     /gene="pcp"
                     /locus_tag="IWK54_000059"
     CDS             70839..71483
                     /gene="pcp"
                     /locus_tag="IWK54_000059"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9592213.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            71562..72839
                     /locus_tag="IWK54_000060"
     CDS             71562..72839
                     /locus_tag="IWK54_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592214.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(73023..73772)
                     /locus_tag="IWK54_000061"
     CDS             complement(73023..73772)
                     /locus_tag="IWK54_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9592215.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            74102..74482
                     /locus_tag="IWK54_000062"
     CDS             74102..74482
                     /locus_tag="IWK54_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9592216.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            74578..75087
                     /locus_tag="IWK54_000063"
     CDS             74578..75087
                     /locus_tag="IWK54_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592217.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            75164..75343
                     /locus_tag="IWK54_000064"
     CDS             75164..75343
                     /locus_tag="IWK54_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592218.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            75398..75961
                     /locus_tag="IWK54_000065"
     CDS             75398..75961
                     /locus_tag="IWK54_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9592219.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(76008..76523)
                     /locus_tag="IWK54_000066"
     CDS             complement(76008..76523)
                     /locus_tag="IWK54_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592220.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(76549..77418)
                     /locus_tag="IWK54_000067"
     CDS             complement(76549..77418)
                     /locus_tag="IWK54_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9592221.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            77544..78224
                     /locus_tag="IWK54_000068"
     CDS             77544..78224
                     /locus_tag="IWK54_000068"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9592222.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            78377..79357
                     /gene="pta"
                     /locus_tag="IWK54_000069"
     CDS             78377..79357
                     /gene="pta"
                     /locus_tag="IWK54_000069"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9592223.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            79480..79953
                     /gene="tsaE"
                     /locus_tag="IWK54_000070"
     CDS             79480..79953
                     /gene="tsaE"
                     /locus_tag="IWK54_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9592224.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            79946..80470
                     /locus_tag="IWK54_000071"
     CDS             79946..80470
                     /locus_tag="IWK54_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9592225.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(80960..81493)
                     /locus_tag="IWK54_000072"
     CDS             complement(80960..81493)
                     /locus_tag="IWK54_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9592226.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(82357..83109)
                     /gene="xth"
                     /locus_tag="IWK54_000073"
     CDS             complement(82357..83109)
                     /gene="xth"
                     /locus_tag="IWK54_000073"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9592227.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            83222..84142
                     /locus_tag="IWK54_000074"
     CDS             83222..84142
                     /locus_tag="IWK54_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9592228.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            84215..85150
                     /gene="murB"
                     /locus_tag="IWK54_000075"
     CDS             84215..85150
                     /gene="murB"
                     /locus_tag="IWK54_000075"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9592229.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            85448..86926
                     /locus_tag="IWK54_000076"
     CDS             85448..86926
                     /locus_tag="IWK54_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9592230.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            87203..89263
                     /locus_tag="IWK54_000077"
     CDS             87203..89263
                     /locus_tag="IWK54_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592231.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            89346..89915
                     /locus_tag="IWK54_000078"
     CDS             89346..89915
                     /locus_tag="IWK54_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9592232.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(90090..90896)
                     /locus_tag="IWK54_000079"
     CDS             complement(90090..90896)
                     /locus_tag="IWK54_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592233.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            91034..92548
                     /locus_tag="IWK54_000080"
     CDS             91034..92548
                     /locus_tag="IWK54_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9592234.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(92951..93934)
                     /locus_tag="IWK54_000081"
     CDS             complement(92951..93934)
                     /locus_tag="IWK54_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9592235.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(93939..94586)
                     /locus_tag="IWK54_000082"
     CDS             complement(93939..94586)
                     /locus_tag="IWK54_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9592236.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(94671..95303)
                     /locus_tag="IWK54_000083"
     CDS             complement(94671..95303)
                     /locus_tag="IWK54_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9592237.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(95319..95870)
                     /gene="coaC"
                     /locus_tag="IWK54_000084"
     CDS             complement(95319..95870)
                     /gene="coaC"
                     /locus_tag="IWK54_000084"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9592238.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(95867..96604)
                     /locus_tag="IWK54_000085"
     CDS             complement(95867..96604)
                     /locus_tag="IWK54_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9592239.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(96601..97590)
                     /locus_tag="IWK54_000086"
     CDS             complement(96601..97590)
                     /locus_tag="IWK54_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9592240.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            97848..98390
                     /locus_tag="IWK54_000087"
     CDS             97848..98390
                     /locus_tag="IWK54_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9592241.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            98407..99492
                     /locus_tag="IWK54_000088"
     CDS             98407..99492
                     /locus_tag="IWK54_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9592242.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            99504..100310
                     /locus_tag="IWK54_000089"
     CDS             99504..100310
                     /locus_tag="IWK54_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9592243.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            100311..101138
                     /locus_tag="IWK54_000090"
     CDS             100311..101138
                     /locus_tag="IWK54_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9592244.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            101135..102208
                     /locus_tag="IWK54_000091"
     CDS             101135..102208
                     /locus_tag="IWK54_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9592245.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            102636..105332
                     /locus_tag="IWK54_000092"
     CDS             102636..105332
                     /locus_tag="IWK54_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9592246.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            105409..105897
                     /locus_tag="IWK54_000093"
     CDS             105409..105897
                     /locus_tag="IWK54_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9592247.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            105909..106403
                     /locus_tag="IWK54_000094"
     CDS             105909..106403
                     /locus_tag="IWK54_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9592248.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            106424..107233
                     /locus_tag="IWK54_000095"
     CDS             106424..107233
                     /locus_tag="IWK54_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9592249.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            107230..108051
                     /locus_tag="IWK54_000096"
     CDS             107230..108051
                     /locus_tag="IWK54_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9592250.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            108102..110201
                     /locus_tag="IWK54_000097"
     CDS             108102..110201
                     /locus_tag="IWK54_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9592251.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            110198..111316
                     /locus_tag="IWK54_000098"
     CDS             110198..111316
                     /locus_tag="IWK54_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9592252.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            111355..112233
                     /locus_tag="IWK54_000099"
     CDS             111355..112233
                     /locus_tag="IWK54_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9592253.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            112424..112576
                     /locus_tag="IWK54_000100"
     CDS             112424..112576
                     /locus_tag="IWK54_000100"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592254.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT        37381 a        19373 c        23538 g        33207 t
ORIGIN      
        1 gatatgcaat tgcaccagag tatcttttca acgtcttagc ggatcaagca aaacaagcaa
       61 cgttccaatt gaacgatcta aacaaagcct ttgtgcaatt ggcatctacc tacaatcaat
      121 ttaatgggtt atttgatgat gtggatctcc aatcaaaaaa actaggaaca gatgagcaac
      181 aacgaaatgt gacgattacc gaagtcatca agaaattgaa tgatgtagat gttttagaac
      241 acgatgggga cgtgatcggg gatgcgtatg agtttctgat cagtcaattt gcttcagaag
      301 cagggaagaa agccggagaa ttttacacac ctcatatggt ttctgatatg atggcgcaaa
      361 tcgtaacgct cgatcaaaag gaacgacgat tctttagtgt ttttgatcca acgatgggtt
      421 caggttcatt gatgttaaac gtacgtaact acttaacaca cccagataac gttaaatatc
      481 atgggcaaga actaaataca acgacataca atctagcaaa aatgaacttg attttacatg
      541 gtgtcgacgc cgaagaaatg aatctccgca atggggatac gttgaataag gactggccaa
      601 cagatgaacc ttacacattt gatgcagtag taatgaaccc accttattcg gctaattggt
      661 cagcagatac gactttctta gatgattctc ggtttaatcg ttatgggaaa ttagcgccaa
      721 aatccaaagc agattttgcg tttcttcttc atgggttcta tcatttgaag gaaacaggta
      781 ccatggccat cgtcttacca cacggagttt tattccgtgg cgctgcagaa ggggtcatcc
      841 gtcaaaaatt actagaagat ggcagtattt atgctgtgat cggtatgcct gcgaacttgt
      901 tctttgggac atcgattcca acgacggtga ttgtgttgaa gaagaatcgt caaactcgag
      961 atgtattatt cattgatgct agtcgtgaat ttgttaaagg gaaaaatcaa aataaactat
     1021 cggaagaaaa cattcaaaaa atcctcgaaa cctacgctga aagaaaagat gtcgagaaat
     1081 acgctcattt ggcaaccttt gatgaaatca aagaaaatga ctataactta aatatcccac
     1141 gctatgtaga tactttcgaa gaagaagaac caattgatat ggttcatgtc ggaaatgaca
     1201 tcaagaagat acgacaagaa cagcaagtgc tagaaaaaaa acttttagaa gctatctctt
     1261 ccctgcaaac aacacctgaa aatgaagcgt ggttacaagg tgcgctggag gtgttcaaac
     1321 atgagcaatg atacacaacc agaaatccgt ttcccaggat ttacagatga ttgggaacag
     1381 aggaagttgg gggacttcgt tgtagattat gttgaaaaga caagtgtaca aaatcaattt
     1441 cctatgctaa cttcatctca acaaaaaggg atagttttgc aagaagacta ttttgcaaat
     1501 cggcaagtaa ctacagaaaa taatattggt tactttgtac tacctagagg atatttcacg
     1561 ttccgtagtc gaagtgataa tgatgtgttt gtgtttaacc gtaatgacat tattgataga
     1621 gggattataa gctattttta tcctgtattt actttaaaat ctgctgattc cgacttcttc
     1681 ttaagacgta ttaacaatgg aatccaacga caattatcta ttcaagcaga gggaacaggt
     1741 caacatgttc tgtctctaaa aaagtttaaa aatatagtag caatgtttcc tagtgaggaa
     1801 gaacaacaaa aaatcggcac cttcttcaaa caacttgacg acacgatcgc tcttcatcag
     1861 cgtaagttag atttgctgaa ggaaacgaaa aaaggattct tacaaaaaat gttccctgaa
     1921 aacggagcaa aagtgccaga aatccggttt cctggtttta cggaagattg ggaagagcgt
     1981 aagttgggag aattaggtaa aactcaatcg ggaattggat ttcctgatgc cgagcaaggt
     2041 ggttctgaag gtactccatt ttttaaagtt tccgacatga ataacattgg taatgagtat
     2101 gaaatgagaa atgcaaatca ttatgtatct aatgagcaaa ttgagcgtaa aaaatggaag
     2161 ccaataaaag atgtgccagc gattatattt gctaaagttg gcgcagcaat aatgcttaat
     2221 agaaaacgtt tggtcacaag ccctttctta atagataaca ataccatggc ttatttattt
     2281 aataatactt gggatatcta ttttggtaaa atattgtttg aaactataaa tttgccaaga
     2341 tactcccaag taggggcatt gccttcttat aacagttctg atattgagaa tatcagtgtt
     2401 aaggttcctg taaaggatga acaacaaaaa atcggtactt tcttcaaaca gctagatgat
     2461 acgatcgctc ttcatcaacg taagttagat ttactaaaag aaacgaaaaa aagcttctta
     2521 caaaagatgt ttgtctagga gggaagaaga atgaaaatta gtcaccgtga ggaagcagaa
     2581 gtcgaagaac agttgattcg tgtacttgga gaagggcata atcagtggac gtatcgtccg
     2641 gatctaaaat cagaagaaga cctttgggtc aatttacgcc aaaaaattat ctcaaataat
     2701 caagcagaac tgaatgattt tcctttgacc gataaagaat ttgaaacgat caaaacggaa
     2761 ttactgctgc gaacgaaaac cccatttgat gctgcgaaat ggttaaaagg tgaaaatggg
     2821 atggctcgga tcacgattga acgggaagat tcgcaattgg gttctgtctc acttatcctt
     2881 tattccaatc aagacattgg tggaggaatc tctacttatg aagtggtcca tcaaattgca
     2941 aaacgtggaa gtaacataga agcacgggat cgacgttttg acgtcacctt attaatcaat
     3001 ggattaccga ttgtccaaat cgagctgaaa caagtaactg caaaagatgg tgtttatcag
     3061 gcatttaacc aaatcaaaaa atatgcagaa gaaggtatgt ttcggaacaa tatcttttcg
     3121 accttacagt tgtttgtggt ttccaatgaa caaaccactc ggtattttgc aaatgcctta
     3181 ccgaaagact tgcaccctaa gtttttattt agttggcgaa cgaaagacaa tgaaaaagta
     3241 gaaaacttat atgagttttg taaacaagtg ttaaatatcc cggatgctca tcgtttgatt
     3301 gcggattata caatcgtcag tgaagatcaa gacaataaaa ccctcatggt gttacatcct
     3361 tatcaagtac atgcgattca agccttgttt attgccgcaa ataaacatca atcagggtat
     3421 gtgtggcatg cgactggttc aggaaagacg ttgactagtt ttgtatctac taagctttta
     3481 gcacgaaaat cggggatcga tcgaacgatc atgttagtgg accgcaaaga tttagataat
     3541 caaaccacca cagagtttac aaaatttgct tcggaattta atactgggat ttcttccggg
     3601 aacgccaaag cgaatagctt gatcgttgga acgggaagtg ccaaagaatt aagtgaaacc
     3661 ctgttggcag atgccaatgc aaatgttgta atcattacaa cccgtcaaaa actagatgcg
     3721 gcgttgaaat atgcaaagaa acaagaagag aaaaaaggaa ccaatcgctt ccaaaaatta
     3781 atgggccaac atatcgtgtt tgtagtagat gagtgtcatc gagcgttaag tgctgaaaat
     3841 atggaagaaa tcaaaaaaat gtttccgaaa tccacttggt ttggctttac aggtacaccg
     3901 atttttcctg agaatcggaa acaagccaaa gggcaattgg cacgtacaac ccatgatcag
     3961 tatggagagg tcttgcatac ctataccatt aagaatgcgc tagaagatgg ctctgtctta
     4021 ggatttcaag tagagcatga aaacaccgtg gagccgactt cactagagaa taaaatctat
     4081 cggaaattaa aagaagtaga aacatatgca gagtattcat cagaacagat caatcgtatg
     4141 attgatcaaa tggaacctgt caaaaaagaa agttatttgg acccagctgt ttttgaagca
     4201 gatgaacata tccaaaaagt cattcataaa atgttccgtc cagataatgc ctatacaaaa
     4261 tttgattttc gaaatggcag gccaacgaaa tctgcaatct taaccacgag ttccattgac
     4321 atggccaaaa agtattatcg ggccatcaaa gagatgacga aagagcctga ttggttaaca
     4381 aaagagtttc ccaatcagct cattcgtgaa ggacgaacga tggaggatcc tgattttcca
     4441 cgaatcgcta ttacctattc attagaagaa aatagccgag attcaagtgc ccaacaagaa
     4501 gaaatgcaaa aaattattga ggaatataat cagtattatg atacttcctg gtcattagca
     4561 gacattgagc gttataacgg agacatcaat aatcgtttgg cacgaaaaag agcggaattc
     4621 aaacaatttg ggaaacaaat cgatttagcg attgtagtgg atcgtctatt aacaggtttt
     4681 gacgccccta cgattcaaac cctatttgtg gatcgtaatc tagagtatgc aggattgatc
     4741 caagcttttt cacgtacgaa tcgtacgtat cctgataaga caaaaggatt gattgtgacg
     4801 tttcgtaagc cagcgacaat ggagaagaat gtcgaggatg cgacaaaact gtattcaaaa
     4861 gcaaaagaag aatcaggttt gatttatcca agttatcagg aatccaaaaa aagattcaaa
     4921 caagcatatg gtaagttaaa agaggtttca tccatggaat ccattgatga acatacgcca
     4981 cttgaaatcc gtatcgagta cgtcaaagca tttcaagaat tgaatcgtgc ttatgaatcg
     5041 ttagtgactt atgacgatta caatgatgac atggtgaaat ctccaacttt gaacaatcaa
     5101 attcaacttt tggaagaaca aattggcgtg tacgagaccg tgaagggttc attaatagag
     5161 gaagaaccag aaaaagaagg agaagatttt tcaacgatcg aattttatag tgaaaatacc
     5221 actaagttgt atgatattga ttcaacttat attgatcaat tactaggaac ttatgctgcg
     5281 aatagttcag atgcacgaga agaaattgaa aaagctttgg ctaaattgaa taaaacagaa
     5341 ggagtcaaag aagtttaccg acaaatttta aatgcatttg atgtggggac tttagatagt
     5401 aatgaagata tctttgtcat taaacgtcgc tattttacac aagcgagtga tgatttgatt
     5461 catgtctttt ccaatgagtg gtttgtttct gagaaagaat tacatgcttc taatctgcaa
     5521 tatctaccag gtgaagatcc aattcctaat atgaaagcaa ttatcaacag taaggattac
     5581 gaaggatata aagccaaaca tccagaagcg aagccattta aatacccaca agaaatgaag
     5641 cgagcatggc gtaaaatgtt agatgatgag ttgattccac ttgagaatga attgaggtaa
     5701 acgagaagag ttaagaaaat tgtacgattc aaagatggta gtcaagtgga agtgtagaaa
     5761 atagggatag atgtctttta tttgttcaca ttttataacg aagtatttaa gacagctatt
     5821 gaataatgtt tcctccacct ttttcattca tttaccccac cccgttgtta tatgaccgtg
     5881 ttttgagagg aaccaataaa aagcataggc taactagggg ttcctttttc ttttgttttt
     5941 ttggaatccg cttaagtgaa atgtatctga aatgaaacgg agataaagtg attttttgct
     6001 ataatgacaa ggttttagct gtttcagata ccatgtaacc caaaatagtt tattgagaaa
     6061 ataggagagt atttgatact aactagtagg aaaaaatgag ataaaaatta aatggaatca
     6121 tttcaggtaa tctcaaataa aaaaatatac agtcctgtgg cactttacct atgggactgt
     6181 atatttttta ctctcagcgg gcttgttttg actccgtgtc tgactccgtc gtattgattt
     6241 taaatgatat tcgatgacca tttaacaggt catttttgaa atttttgtct ctatatgact
     6301 cagtttgata attattgata gatcaatttg ggtctatatc gaacaaggga ataatccaat
     6361 aaaaaatgat ttagaatatc aaaataacaa aaaagaggac tacctgtatt cgtcctcttt
     6421 tttgaccaga ttttgaccag tctaatttac attctaatat attcatatga gctaattttt
     6481 tgattaaatg gtaagttttt ccttgtttgt tttagtttaa actagtgtgt aatttaaaaa
     6541 tctatatgga agggtctgtg gggtaatact taaataaagg ctaggaacct gataacatca
     6601 aggttcctag cctttttata ataaaatgaa gcgaaaataa tttcttttaa agagccgaga
     6661 ttatcagcta tataagagtg aatttgatgc aaaaaacaga gccgaaacag tcttggtgtt
     6721 cgtagctctg cttatatgtt tcgaatttaa gacaaagagt tggcaaaaga tgttgataag
     6781 gaagataaga taactgtgtt atataaacta ggtaactaac tgtaacaagc taattttaag
     6841 agattcttat tagaagacgt ttaggaatat aactaagaaa atgattatac aggtaattgt
     6901 catttcaata atgttttcta attttcgcgt ctttgtttta agaaaaaggc cagaatcagt
     6961 tggaaaaaag ttcgtacgat agtgaagagg ggcaaaattt cctactaata ttcccactaa
     7021 caagagataa aacggccagg ttggtgtgat tcctctataa aaaaatgccc atgctaccga
     7081 aatcccatag attaacaact gaacaatttt tacaatgaaa aaattgttct gccattactc
     7141 aaagtctctt cctccttgta tcgtttattt gtttcatttt atcattatga taggcgcttg
     7201 aacaatccat tttgtatttt attgagtact cttggataaa ctaccagcga ttatcatatt
     7261 tatttatgtt actgtaagcc atcatataaa agtaaatgag cagatcatag agatataaaa
     7321 aagaactaaa attgatctaa attatgtgca gcttgactac gtaccaatct atattactta
     7381 ctagattggt cgttaatcta ttcggatcga atgagcaaaa tatttttgtg aatgtccatc
     7441 cttgattatg attccaaaaa gaatcattgg gaggctgtgg cgtaaatatt atactataag
     7501 ccagaaatac ctatcattct gaactttttt atttaggaac tttcatctca aggctttttc
     7561 aattaatttg tacccctgtt ttttggataa gatcatgtgc attgattttt tctatctcta
     7621 cgttcatctc ctacttaatg cctatctggc tcctgagggc ttctttttaa ggcgtcgtca
     7681 tagtcttggc tgaggatttc ttggtaatct gagggaatgg cttcccaagt gagcaggcga
     7741 acgttcatga cgtgttttcc tttgtgtttg attacagcat ggtttttctc tttttctacc
     7801 tctccagcgt gaaaagacag ccatgtacca tggccaaaga tatcattaca tacccaacgt
     7861 tcgatggaat tgtccacgta tcctacaggg agtgtggtat agccgatttg tggtttcaaa
     7921 aaaggattga atcgatcgat cgtttcattt ttgtattgat aattgataag aaaaagatag
     7981 accaaacaaa agacgagcag aaagaaagat agtgtctttt tgagtgagaa gggttgtttt
     8041 gatgaataag atttcttgtt aacaagaggt tcatctggca atgttggttc tattgattcg
     8101 gtagctgatt caatagaagg aaaatcaatc ggcagaccta aagtatgatg taaaagtgac
     8161 agcatttcaa ggtctggcat tgtacgagaa ttttcccaac tagaaatcgt tttatcggaa
     8221 acaccaattt tttcgcctaa ttctttttgc gtaagcttac aatttctgcg gtaagttcgt
     8281 attttttctc cggtgttcat cttcttctcc tttcgattct acagtatata gatttattct
     8341 acatgaccta tacacttgat agatacgttt ttttgatgaa aaacggtatg attttatcaa
     8401 agaatcattt aagtaaggga gagtggtgat aatcaaaaaa aagttcacag ttttactcac
     8461 aatcatagtg accggtatca tgagtggttt tgccttggtt agtataaata aacaggtact
     8521 ctatcggaca aatgaatttt tccatcaaac agatacttat actccgattc gattgccaca
     8581 acattttatt gggcaagaag aatatgagca gctaatcatg gtatttgaaa atgtctcatc
     8641 agaaactgag ctttcttata tgaaaaaaac agtaaatgaa tccaatgaag tagatacatc
     8701 ttggaacttc aagatacagc caaagcttga aatgacattg tattatcaag gggatgtcaa
     8761 atcttctcac gagaaggtac cctattctaa tacctccttt cgcgtaacgt ccattgaaga
     8821 atcgatcaaa ataaaagatt ttgaaggtga atttttcttg atgacgagag aaaaagaagc
     8881 ctatgaacgg ttcatttcac tattcactga tcgattcaat caagcatttc acactgatta
     8941 tgccactaat atttttgaag actttgaaaa tagctattcg tattcggtta tttcaaatac
     9001 agacgtatat aattatcgag tgtatttaac tattggattg attcttttat tccttttgtt
     9061 atcatccttt ttcttattga tgaataaaga aatcagttta ttgttgaata atgacttttc
     9121 attacatcaa gtattgtggt gtttattggg gaaaaagaaa atcattatta cattcataat
     9181 tgcaggaatc attggtggtt ttatttttat gtattataag gagcaatttc tttatttgtt
     9241 ccagatgtac gttttcttgt tcagcggaat atatggtatc tctttgattt ctcttttatt
     9301 agttgaaaaa atcgcttcgt taagagaagc gttcaactat atccaataca ttctttttac
     9361 atttggattg ttgttttctc ttcttttgac cgttgccaat gtcgaattag caactatcgt
     9421 tttttcttct tcacagttag ctgtttccca tgaaccacca aaagaaatcg ctggtgagga
     9481 ctatcatgta ttttatccag taaccatcgg gaaaaaccac gtggatttta tttattccaa
     9541 tcgttttgca tcagcagcag atcaggaatt atatgaaact ttgaataaaa acggaagtat
     9601 attagtcaat gtatcagatt acttgaatga agaaaatgaa cagtttggca gagggataaa
     9661 aataaatctg aattatctta agaaatttcc tttgattgat gtctctggaa ggttgatcca
     9721 aataaccgaa aaagaaacgc accctgttat attgatccct gaacgatata gacatacaga
     9781 tttaaaaaat gacctggcgc aaataacaga atattatcaa gaaatcagtg aaaaagaacc
     9841 gaaatttcta tttataaaaa acaatcaacc catttataca tttattcctt ctatcccctg
     9901 gatcgaacat tatccggttg ttgaaatact gacattgaaa aacagtatgt atttggaaag
     9961 aaatattctc agcggagaaa tctatccacc gttaaagatc aagataggct ctttgtcgaa
    10021 agaaaggtta tttgagctga ttgaagaaag tcagttacgt gataacttgc agttatcatt
    10081 tccgtatact cagactgaag agatagctgt caaagtgtta agcagatcat ttcattactt
    10141 gatacagata tttttactca ctttcttttc tttcttcttg ttgagctatg tcatgttatg
    10201 catatatttt gtctacaatt gtcggaaaat cgctatcttt cgttcaagtg gttattcgct
    10261 ctttgaaacg tataaagatt tcttcatgat gaacctcatc aaatggggaa cgacaagtgt
    10321 aatcttcctt tttcttatag aaagagagcc taaatatctt tttaatattt ttttctttag
    10381 cttcattgat tttattcttt cagttgtttt tattttgtct acagaaaaga aaagtcagtt
    10441 attgctgatg aatggaggtt aacaattgga gccgttgata gtgataaaag gtgcgaaaaa
    10501 aatcattcga aacaaaacga ttcttcattt ggatcgtttc actgcggatg ctggggatct
    10561 tgtgactgtt gttggagaaa gtggagcagg taaaagtacg ttattgaata tattaggatt
    10621 gaatgatcag ttcagttcag gacaattcta tttgttcgat caagagacga aacaattaac
    10681 tgggagagag aagcgcttga taaaaagaaa taccatatct tttctttttc aagattttgg
    10741 gttgattgaa tatgaaacaa tcgattttaa tttagatatt ggattgaagt tttcgaattt
    10801 aaatgtttca gataagcgaa cagcaaagta tgaggcattg aaaaaagtaa atataaacaa
    10861 gaagctaagt acgcctatct acacattatc tggtggtgag aagcaaagaa tcgcctgtgc
    10921 gagagtctta ttgaagaata gcaaaatcat tttagccgat gaaccgactg ggtcattgga
    10981 taaaagaaat aaggataaag tagttgaaat cttatcgcaa caagcttccc aaggtacgtt
    11041 agttatcgta gtcacgcatg atccatatct gaccaaaata ggaaacaaga agatcatgct
    11101 ttaaaaatca aaaaggaaag gatgtcgttt tatgaaaaaa gcagttatta tttgtgcact
    11161 agggataggt tttgttggag gaaacgctat gtcatctgaa gtggaagcaa attcagttca
    11221 cggtgaaatc tattacaaat ctttaaagcc aggagagatt cattttgatg ctaaaggtaa
    11281 tattattaag tcaaatttac cagtagaccg ggaaatttct agaacagcaa ttaatcatag
    11341 ctctggaaga tgggtttatt atagtgatac aacttgtacg agaaaaacag gtcattctaa
    11401 ccactatagt cgtgtctata atcgtcacgg tgcaaaagcg aaagttggaa actctattcg
    11461 atcagcaaat gcaacaaaag gagtatgggc ctatgcaagt gcgacaggaa atagaactga
    11521 cacattctca tgttggtaca atccaaccgg ttggtattaa gtatccaaaa gtttatataa
    11581 aatagaaaaa acatataatg aaacagcgtt aaaagttatt tttggtagtt ttaatatatt
    11641 catttttagt tgtataaaaa tatatcataa taaaaacaac aaaaaaatgt ctggaaaatc
    11701 gaacataaaa gtgtctgatt tgaatatccc agcagcggaa gaagaaaaac aaaatcgata
    11761 acatttgttc taagaattag ttacgttaaa tacggaacgc aagatagact tgaaaaacgg
    11821 taacaaaaga agtagtatat taaggtgtga tgtgctactt gacatttatt ctgctgagcg
    11881 aactttctgg attgtgtagc cttatatcta ttcaggacgt atcgagcaga ttagtaaagg
    11941 tgttcttgat gattttcatc ttgaacacct ttgtttttag caattcgcaa aaaacgaaaa
    12001 gtaggagaat tgataaatga aaaaaagatt tttagcactt gccatcgtac taggcactgg
    12061 cttattgtca ggctgtacga atgccggtga aaaaacagcc gttagttata aaggcggtac
    12121 aatcagcgaa caagaagtaa tggatagctt gaaaaaaatg caaggagcag attccgctgt
    12181 gcaacagttg atcgtttatc aagtatttga agataaatat ggagatgacg tatcaaccaa
    12241 agaaatcgat tctcaatatg atcaaacaaa gaaacaattg ggtgattcat ttgattcaca
    12301 attgaaatct gcaggttaca cagaacaaac attcaaagac agtatcaaac aaagtctagc
    12361 ctttcaagaa ggtctaaaga aacatatcaa attgaccgat gaagatttaa aaacggcttg
    12421 ggaatctttc catccagaag tggaagctca aattatccaa gtagcaagtg aagatgatgc
    12481 aaaagatgtg aagaaagctg ctgataaggg agacgatttc tctaaattag ccaaagacaa
    12541 gtcaactgat actacgacaa aagaagatgg cggaaaagtg aaatttgatt ccactactac
    12601 aacggttccg gcagaagtca aagaagcagc ctttaagttg aaagatggtc aagtttctga
    12661 tgtgatcact tctaccaatg cttcaactta tactacggaa tattatgtgg ttaaaatggt
    12721 gaaaaaccaa aacaaaggca acgacatgga taaatacaaa aaagaattga aagaaattgc
    12781 aacagacaca aaattaagtg acagtacctt ccagaataaa gtaattggcg aagtattgaa
    12841 agacgctaat gtaaaaatca aagacaaaga ctttgaaaat gttttgagta cattcacttc
    12901 agattctagt acagcaagta gtaaataagc ggtataaata aaaaatatga actatttaaa
    12961 tcaataaaac tgttcatcta aatcttttta ttagaactaa atcaacagta gtttaattac
    13021 tacttgattt agttcttttt attttaaaaa ttttttttac ttagaatcca gcgaccctag
    13081 atatttttct gtataatctt aaataaagaa aacaatatca aaaaatgcac gtagaagtag
    13141 ataaatggag tggttttgca aatttctttt atttctagaa gttattcacg tatacggatt
    13201 tgtttttctc acaaataaaa aggcaggaag tacaatggat catttgtata taaaatatat
    13261 tcatcaattt tccgttttgg tgttaggcac actaattata gcggggattg tcgcatttat
    13321 tattcaaggc cgtgacatat taacagtaca tcaagagttt ttcagttatg catttggtat
    13381 tcctctgatc gctataaggt ctgataacaa aaagtggaag aaacgctatt acattctctt
    13441 gctatttttg tgtttttatc tgccacctaa gatttttact ttattaggta tacaatgcct
    13501 atcattttct tttatttcct atatcattgt tttaattttc ctcagtttca tagtatggat
    13561 gttttatctt gtcaaaaaat aaatgaatgt ttcatgaaca gtacttcatc acagcctaat
    13621 ttttttcttt atgctatcgt tatattaaag aaatcaatca gaagggaaag aaagtgatga
    13681 aaaaagtttt attagtggta aaccccagtt caggcggtga acaagcaaag gaatttgagc
    13741 aattagcgat agcaaaattg gaatcggtgt ttgatgaagt ggtcgtcttg catacaaaaa
    13801 aagcagggga tgcaaaaaac tttactcgcg aagcggctac ggaagggtat catagtgtgt
    13861 ttgtcatggg cggagatgga acagtcaacg aaggaatcag tggaatcgct gaacaagaac
    13921 atcggccaaa tttcggattt ttcccattgg gtacggtgaa tgacttggct cgagcattag
    13981 ggataccgct agaacctgag gaagcgatca atcatttttc gattgaatca gtcaagccat
    14041 tggatattgg aaaaataaat gacgactatt ttatgaatgt cgtcgcaatt ggctctatcc
    14101 ctgaatcaat caatgatgta gatgcggaga agaagacgaa atttggaaaa ctagcttatt
    14161 tcatgtcagg aatcaaacag ttagcttcca cacaatccta ctcgtttcac gtagaagttg
    14221 atggaaagaa ggaagaaatt gagagcagta ccttgctgat aggactaact aactcagtcg
    14281 gcggttttga aacattactt ccaaatgcaa aagtggatga tggtaagctc catttggttt
    14341 atttaaaaga ttcttctttg atcgatacgc ttaaaacatt gccagaccta ttaaaaggag
    14401 tagatgaatc tacgaaaaat ctagcctacc ttacttgtga gcaattagtt gtgtcattag
    14461 ctgatgagga agaattgacg acgaatgttg atggagatga aggggataaa ttacctgtga
    14521 caatcaaagt attgccctca catttacagg tttattgtta aaatggaggt tgtgaccgag
    14581 gagttggcgg gagaacaccg cttgttcggg ataagggact gtgacaagac ttttgtcaca
    14641 gccttttttt tgtacaaaaa agaaatttac acctaataga aaaatttttc cataaaaata
    14701 catattttaa atatgaaagt acaattggaa atagattaaa acgataaaat aaaattgtca
    14761 aaacgtcaga gaggatggtg gagaatgaat caatgtgtaa aaaagctgat tgcgtcgatt
    14821 ttgtttgtct tccctctctt gatggtgaca tccgtttttg ccgatgtaga gaaaacaaac
    14881 gaatcaacgc aatcagagaa tcaaagtttt gacaagacta ctttttcaga agaatcgatt
    14941 acttctgaaa ctacagaatc tcaaagtgag gcttctacta ataaaaggca agaagaaaat
    15001 aagacaagtg agacctcaga agtagctgaa caaacgtctg atagcagtga agcggcactg
    15061 gcaagagctg tttcagcgga tgggtacagc gaagctgcca caacagaaga actggcgcag
    15121 cttctcgcag acgaatcagt aactaagatc cgactgattc agcctttgac tttagacagg
    15181 gaattagaga tcaaacgaga tatcgttatt gattttggtg gattcgctca caatttcggc
    15241 acacaccata tttatatcaa tgagatacct cagtcgatcg agttccagaa ttttaaagga
    15301 acagctgcac atccaggtgg attgacacca aatatcgatg gaaatgccat tatcattgct
    15361 tatatgaccg atttttgggg aagcagatat cattttacgg gagaaataaa atttaccggg
    15421 acacttgatt tgtatgatgg gtccaaatta ggactgattt acgctcctcg tgcaactgtc
    15481 acattagatg gtgtttccgg tgtgttggat gtccaaccag tgaaggaagg actgaatgcc
    15541 gctccgggta aagcctattt ttgtagaagc tatcaattga atgtgatcaa tggtagtcaa
    15601 ctttatggac cgtatctagg gaaattctac ggttttctag attcaggaga tgagactggt
    15661 agtggaaaaa cagcgccagg aatccatatc ttaagcggtt caaaggtctc tcttgattat
    15721 gatcgggcag atggcgttag cgatgaagga gaggcagtcg atacgctacc tggtaatgtc
    15781 atgtttgaag tcagtggttc gggaagcgaa ttttcggtaa atacaaagat caatattacc
    15841 aatgacaaca atcgcggaat catccaaatg agaggttcag gatcgaaagt cactgtatcg
    15901 aatggtggga aaatcaccat caatacagca actacgggcg gatttcgctt gcaaggagat
    15961 tctagtcaaa tccatgtatc aagtggtgga caaatctcgg tcaaaatggc tggagatgac
    16021 gatcaacccg ggaacaatgg gatgcgtttt gtaggaaaag gcctttcttt gatcgtagaa
    16081 ggagccggat cagttatcga catcgataag cagagtgggc gttccagcgc agttcgtttt
    16141 gagaatggta cgcaaaagct gacagtaacc aatggaggct cattgagggt aagaaataca
    16201 ggtgatggca aatcttacgt gaacagactc aatcaaggaa atcaagcgat ccaattttat
    16261 gacgcttctg gttttctaga aagtccaggt tcagctgatt ttacagtcac tggagaaaaa
    16321 tcaaatatcg atatacgcgc agattccgga gcaactgtag atacaactgg agatattgat
    16381 ttaaatttta cggcaggtga aaaaacttat ttagtcatgg tcgggaaaac aggggaagat
    16441 aagaccggca tcatcagtgg ggatgtgttg aatgtggact tagaatctcc tacctatttt
    16501 gatttccaaa acaaacggat tggaagcgag accaatggca taggcggctg ggtattccaa
    16561 ggaaatggag attcttcttt aaagatcgcc cattcagaaa tcgctttatg gcgaaaaaca
    16621 ggcgcaacat tcgataactt tgaaaaagac ccggattatt tttctgatat gacagactta
    16681 tcgatcaatg ggaccgactt aggcaatttc gtatcaagcc ctagtgcagg tttaacaaat
    16741 gaatttgctc agacaggtgc cggaatgaaa aattataatc gaatcagtgc caataatcag
    16801 ctcccgatga ttgaatcctt aagagtgcca acgaatgcag ataaaaaaat ctatgggcat
    16861 gtcgttgttc ccgaaggtgt cgaggcaaag ccaagagatg cttggacagg cgaagtagaa
    16921 gtgactttac gtattactta cgcagatacg acgaaatcat ctgtagaaat tactgctctc
    16981 acgcaaggac aaacagatga aaatgaaggg gcaggatata acccatgggg agaaggcgaa
    17041 caaggcggct tgttcgaagt tcaagtaccc aatgatgaga gattaaaaac ggaggatcaa
    17101 gtgcaagttg ttgctgctaa aaaagtaaga ggtggccaag aagtgacaga actggactct
    17161 gccaaaacga cagtcgatgt gattcctcct gaacctgcta atttaagtac cacattgatc
    17221 aatgcagctt ctcgcacgat cacaggtaca ggtgaagcag gagccttggt ttcattgaaa
    17281 aaaaatcaag ctgttttgtc agaaacaact attgatgata caggggaatt tgttctttct
    17341 atcccagaaa atcaactttt tgcaggagat gtattagaaa ttgtattaaa cgaccaagca
    17401 ggagaagcta ctgctaaggg tgtgcagaac aagccaagta ccaacgatga aattgggaat
    17461 cacaatccaa gtgatacgcc agttgtttat catgatgccc cgccttttgc acctgctacg
    17521 aagatcgtag tagaaggtgg tctctcattt cttgcccctt ccaaactcga ttttggtcag
    17581 atcaaagcaa ctggtttgac acaagaagca tttggccaaa tggatataca ggaaaaatta
    17641 tggatttatg atcagcgaga aacgaaaaat ccgtggatct taacgataaa attatctcag
    17701 cctttcacaa atgatgcagg attttctatg caggatgtcc tatatttcaa gaaaggagag
    17761 gagtatcaat tagtgaatga agaagaactg gaaattgtca gaggacaaaa ttcaacgaat
    17821 gtgcaagcag attattcaga atatttaaat aacggtcggg cgtttaaact tgtcttaacc
    17881 aaagaaaatc agatagctgg taattactca gcagagataa catggacatt agcagatgcg
    17941 ccgacagaat aaagatcaag gaggatgatt gatgaatcga atatggaata aaagtcatcg
    18001 aaacaactgc tctttactta tgatccttct ccttggattc atcctttggc agacacctgt
    18061 ccatgcctcc gaattcaatt ttgcggtaga ttttgaatta ccggctaatc agattgataa
    18121 gaacaaaaca tatataaatg taaggatgga gcctgggaaa acacaggaaa tcccctacac
    18181 ggtctttaac cgaacagata aatcagtaac agttgagaca ttgatccact ccgctacgac
    18241 gaattcaaac ggtgtgatcg aatatggaga atcaaaaagt aaatttgatg aaacattaag
    18301 ttatgatatg aaaaacttag caaaaacagt gggagaaata actgttcctc ctcaaagtgc
    18361 ggtcactgag acactttcac tgaaagcgcc agaaaaagag ctggctggtg tggtcgcagg
    18421 agggatcacg tttcaagaaa aaaaagagaa cgtttctaac aaaagtacag ttggtgtcgt
    18481 caatcagttt tctcaagtga aagcaatctt gatttgaaat agcgaaaaag agtcttcccc
    18541 agatttgaaa ttaaaaaaag cttatgcgga tcaaagcgat ggaagaaatg tgattaaggc
    18601 gaatcttcaa aatccaaatg cttcttatat gttcggcttg actacatcgg ttgaaatcta
    18661 caaaaaagaa acaaaagagc cttacctttc aataaaaaaa gaaaaaatag atgtagcacc
    18721 caatacaaat tttgatttac ttgtcccatt agaaggaaaa aaattagctc cgggacatta
    18781 tcaagcggtt gtgaagggtg cttggaaaga aaaacgctgg aatctccata ccgattttga
    18841 aataactgat gatgcagcgg accaattgaa tgaaaaggat attgatttaa aaaatcagcc
    18901 agaaacattt gactggcgat ggctgatact tgttggagtc attcttattg gcttatttgc
    18961 tggattattt attttaaaag aaagaaggaa aaagaaatga aaaaaacaat tgcattaagt
    19021 accgttctag ctggaagtct attattatgt acacaaagtg tttttgctga gaataagagc
    19081 tcagccacta cagaaggaac gatttctgtg accaaaccag atgctgcaaa tcctgaagac
    19141 gttgttccag ttcctcctgt tgatccaaat gatcctgata ctccttttac acctgacgaa
    19201 ccaaatcagg gaacatccgg attactgacg attgaccaag ctccagatct tgattttgga
    19261 acaatcaagt taggtacagc aaaaacagta tatgctgcat tacaaagcgg aacggacagt
    19321 actggagcac aaacacaagt acagaatttc gtacaagtaa cggataaaag cggaaattat
    19381 gccggttgga aattatcagt caaacgaaca gaatttattg gtacaacatc tgatacgaat
    19441 aaactaactg gttcaaaggt gaaattcaaa aacgccgtga ttcgaaatag cgcaactaat
    19501 agcagtggaa tgccatctac cgtttctgga actggcgctg aaggaatcga aatcccagca
    19561 gatcaggcag tagatctagt aactgcagga gcaaatgaag ggattggcac atggatttat
    19621 tcattaggag agaatgtgga acaagggaga caatcagtgg aattatttgt tcctgtatcc
    19681 aattatgctg tagacaacta cgtttcaaca ttcacatggt cgttaacaga tgctcctaca
    19741 gaagaggaag cgtaaaatca tatcaacgat ttcagggagt ggagccgatt caaggcccac
    19801 tccctgaaat ctgctatgct tacgtctcag ctcttattca tcaactcgaa gcagtttttt
    19861 ggaagtcatg gaaacgttcc tttgtctttt caaagatctt tcgaccaacg agtaaggagt
    19921 caccgttgaa aaattctaac acataatgag cagaatcaat tttctgcaat tgcgataagt
    19981 tgatacagta agattttaaa agtaaaaagt aagcatactt ttctaactct gtcttcaatt
    20041 gttttaaaga agtattcact aatttatctt ctgtagttgt tttgaccata acttggtttt
    20101 tcaaaccttt gatactttca atatataaaa tttcccgagg atacaggacg agacttccac
    20161 ctttttgatc aataaagatt ttatcgacat ttacgatctg ctgttctttc caattgatct
    20221 gttctagcac ttgacgaatt tgttcagata aattcctgtc ttttctgata tatccaatcg
    20281 gaaggatttt ccgattgatg atctctactg caaaattcgt ataagcggtg aaaaaaacaa
    20341 tattactatg tgcatttttt ttacgtattt gttcggcgac atcaatacct gtgagtgatt
    20401 gcttcaagtc gatatcgata aagaaaacat cgtgattatc aatagtgccc aactggatca
    20461 actgattgat ttgagagata gaagtgagct gcacgatatt ctcgatatga tgattgacca
    20521 attctctgat gatccattcg ctgtgaaact ggttatcttc tatgataaaa tactttttca
    20581 tatgcaggct cctattaaaa aaatatgtgt acattttgaa acaaaacaat ggaggaataa
    20641 gatggtcgca acagtgacac tttatttgac ctttactttg attttagatt taggtatcta
    20701 tttattcttt tcgaaacaac tgctagtaaa aaacagcttt ggttgtatag ctttgatggt
    20761 ggtgacactt ttacttccta tccctaaagt gagctttcct tacctagttg gcttattatt
    20821 gatgtttctt ttttatttta ctacgaaaaa cctgtttatc agctgtttga tcagtaatct
    20881 tttttttgct atcactggac tcagcttttt tctggttttt gatctgatgg attatttagg
    20941 aaagagacat cttggaacag caagtttttt tgcactactt atcgcattta ttttctatct
    21001 attagtaaaa agtatagaca aacagacgag atttctcaac ttgcttttta cttatccaac
    21061 tgaacactat tttggaggta tttgtatttt attaagctgg ggcgtgattt acttggtatt
    21121 gggaagtgtt cacttccgct catctaatta tctgcttcta tctcttttgt cgatagtcat
    21181 cagttttttt tctttgtttt tttccattat gttgatcagt ttccaagtaa aagaaagaca
    21241 acatcaggaa tcgcttcaac tgtatcaaat aaatgaagaa tattaccatc atctagagga
    21301 attcaaacat gattataaaa gtctactgtt ttcattaaaa attactcttc aggaaaataa
    21361 aaaagaaacg ttggaatttt tagaaatatt agaagatcat tctgcagcta tttttgaaaa
    21421 accgcaaata caacagctaa caaatattgt atccccagct gtcaggggtg tctttttaaa
    21481 atttctagaa aaagcagaat cggagaaaat cccgatccaa gtagttattc ctgtcgaagt
    21541 gaaaacaatc ccgattgatc tggttgtttt tattcgctgt ttatctattt ttatttctaa
    21601 cgcattcgat catcgtgtag ccgatcagag tccgattacg atcaatctgc tggaagaaaa
    21661 agagggatca cgttttagta tagcgaacaa ggcaaatcca acgaatttga cgctatcgga
    21721 gatggtcaaa agaggaaaaa cctctaaaaa aaatggcggg ttaggtctat acagtgcgaa
    21781 aaaaatgttg gatgtttacg aaaatgcaga actaaaaatc gaattttcaa aacagaatca
    21841 ctattttttt gtagaaatgt atctggcatc tagaaagagc aaatcagcct actgacttac
    21901 tgtcaaagga taagtttgag gaaagtctac acggagatag agacaacaac acagcatatt
    21961 caaaagaaac agaagcgtga tgaccgttgt cacccttctg tttcttttgc tatgtagtta
    22021 aacaaagtag ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact
    22081 aagtagataa gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta
    22141 caccagataa agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc
    22201 tacgatcaca aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat
    22261 ccatcgaaaa gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg
    22321 aaaatcatta agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa
    22381 acaaaaagcc tcaagggaag aagacacgct actgcataca catggtggct acctaaatta
    22441 ttgattttct atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca
    22501 tctatttttt atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc
    22561 aagcaaatga atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa
    22621 tttcataacg aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa
    22681 ctttttcaat ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa
    22741 gaactggcag atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta
    22801 gatgagatca ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc
    22861 gcaggtgtac gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga
    22921 gcaaaagaaa aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc
    22981 aaaaagatgg ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat
    23041 tttcaatgaa cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc
    23101 gaatgtaaca aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga
    23161 tgcggtagaa ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct
    23221 accagtgatt gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg
    23281 cttggataat acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg
    23341 aaaaggaaat tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc
    23401 gctagaaaat ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac
    23461 atacaaaaca ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt
    23521 cgagggaaaa gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact
    23581 agttgtacaa ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag
    23641 atctgctttc aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga
    23701 ttaaaaacag ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga
    23761 aacgcaaaga ggtgcatctc tgatttggtt gttttctaga tagacttgtg ttccagtttt
    23821 tttatttttt atatgaaaag gcttgcataa ataaaaattc gatggtatga tgattttgaa
    23881 taagaaatgg attgttactg tacggcaggc aaaaccagaa ttcagctaag aaacagtagg
    23941 cgaattctag gtttttttat ttttcttgag agggcgaagc aaaatgaaaa ttgataaaat
    24001 tttgaataac aatgtcgtca tttcgaaaaa tggattcggg gaagaagtcg tttgtatggg
    24061 aaaaggacta gcttttcaaa aaaagattgg ggacgagatt tcgccagaag cggtccaaaa
    24121 agaatttgtt ttaagagatt cttttgctaa aaaccaattc cagcagctga tggctgatat
    24181 tccggcagag gagatcgaat gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa
    24241 agttgaattt tcgcctaata tttatctgac attgaccgat catcttcatt atgcgatcac
    24301 acgagcaaaa gaaaatatcc aactgcctaa tccattatta tttgaaacaa aaaaatttta
    24361 tccgaaagaa tatcaagcag cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt
    24421 ggaactttct gaggatgaag ccggttttat tgcctttcat tttgtgaata gccaacaagg
    24481 aaacgaagac atgcaagtga cgatgaccgc taccacaatg gtaagagata tactgagtat
    24541 catcagtaaa ttcttcggca tcgtgttcga tgaagaatct ttgaattatc agcgtatcat
    24601 tactcatctg cagttttttg cccagcgtta tctcaaaggg gaaggatctg acgagcaaga
    24661 tgaatttctc tacgcattag ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg
    24721 gatcaatgag tatcttcgac aaacgcaaaa acaggaaatg gggatcgcag agcagattta
    24781 tttaacgatt catattcaaa gagtagtaag tgagaaaaaa agcatccaat cataaggatt
    24841 gttactgtat ggcaggcaaa acctggattg aaaaaatagg ttcacgaatg tttgtgactg
    24901 tttttttcaa tctgggtttt tattttttaa ataaaaaaga aagaaggaaa aaacaatgga
    24961 taatcaagca gttggtcgtc gtgtatggga agcggtagga ggacaaaaga atgtcaaaag
    25021 tttagtacat tgtgctacgc ggttgcgatt cagattaaaa gatgaatctt tagcagatac
    25081 gcaaaagtta aaagaagacc cggacgtgat ccaagtcgta caaagtggcg gtcagtatca
    25141 agtagtgatt ggcagtaatg tagcagatgt ttatcaatcg atcgtggatg aggaaggtct
    25201 tgcagtttcg gaggatggaa aagaagagtc caaaaatatt ttgaatcgtc tgatcgatat
    25261 tatctctagt atatttacac cttttctagg ggcgatggca gccgccgggg tattgaaagg
    25321 attcttatca ttggcaacgg tgatgggctg gctgacagct gatagcggta cttatcaaat
    25381 attgtttgct gcagcagatg gtgtctttac cttcttacct gtcatgcttg cttttacagc
    25441 agctaaaaag ttcaaagcca accagttttt agcagtagca attgctatgg cacttgtgta
    25501 tccagctatc acagcagttg ctggtgcggg agaagctatc agtttctttg ggatcccagt
    25561 gattctttca ccatctggtt atacttcttc tgtcatacca atcattctag ccgtatgggt
    25621 tcaaagtaaa ttagagccat tcgtaaaaaa agtgattccg caatttctgc aaatgatttt
    25681 agttccgctt gtcgttttag tagtgatggt tccattaaca tttcttgctt taggaccaat
    25741 cgggacggtt gccggtaatg ctttaggcgg tctcttcaac tcgatttatg gatttagtcc
    25801 aatcgtagca ggtctaatca tgggaagttt atggcaagta ttcgtgatgt tcggcatgca
    25861 ttggggattc gtaccgatca tgtttttgaa tatcgaacaa tatgggtttg atgtattgat
    25921 gccaatgctt cttccagcca tcttggcaca aggtggggct gctttagctg tagcattgcg
    25981 aacaaaagat acgaaacttc gagcattagg gatttcttct acagtgactt cgttatttgg
    26041 aatcactgaa ccaactgtgt atggtgtgac attacctttg aagaaaccat tcattgccgc
    26101 ttgtatttct ggaggtattg gtggagctat tatcggattt tccggagtaa aagctttctc
    26161 cagcagtttg gtcagcttat tgacgattcc gactttcatt aatacggtgg atggtgtaga
    26221 gtcaaatgtg acagtagcag taatagctac aggaattgct tttgttcttg cattcgtagg
    26281 tacattgatt ttaggatttg acgaacaaac acaagacaat caattagaaa acaaacatgc
    26341 gaatgcagga aaaccgatta cttcagcacg gcatacctta aaaagtcctt taacgggtaa
    26401 agtactgcca ttgagtgaag taccagacca agttttttct tctggcgtga tgggaaaagg
    26461 tatcgctatt gatccagaag taggagaact agttgcacct gcagacggag agatcacgac
    26521 tatttttcca actggacatg ctgtagggat cacaacgact gacggagcgg agattttgat
    26581 tcatatcggg atggatacag tagaattaaa tgggaatggc tttgagatct tagtcaaaca
    26641 aggggatctc gtcaaagcag gagatttatt gattcgtttt gacatcgaag caattagagc
    26701 agctggctat agcgtaatca ctccagttgt cattacgaat actgatgcat ttgccgatat
    26761 acttgaatta gaccaaaaag agattattgc aaacgaagat gttctggcaa ttgtaaaata
    26821 aggaggaaat caggatgcaa tcacgttttt cagaaaaatt tttatggggt ggagcaactg
    26881 ctgccaaatc aattagaagg ggcttatgat cgagacggaa aaggactatc cgttgcagat
    26941 gctatgcctg gaggtaagca gcgctttgcg attatcggca gtgaagaatt tgactggacg
    27001 attgatcaag aaaaatatat ttacccgaat catcgaggaa tcgatcatta cgatcgtttc
    27061 aaagaagatc ttgccttgtt tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc
    27121 tggacacgaa tttttccaaa tggagacgaa ggtactccta acgaagcggg attagaattt
    27181 tatgatcagc tgattgatga gtgcttaaaa tatgatattg aacctgtgat cacgatttct
    27241 cattacgaaa tgcctcttca tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg
    27301 atcgacttct atgaacgatt tgctcagaca gtattagaga gatacagcag taaagtcaaa
    27361 tattggatga ctttcaatga aatcaattcg gcctttcact ttccagcttt aagtcaagga
    27421 ttagtaaaaa gcaatggagc aggagagtac caaaatattt tccaagcatg gcataatcaa
    27481 ttcgttgcaa gtagcaaagc cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc
    27541 ggctgcatga ttatctatgc aacaacttac agcatcgatg ccaatccagt caatcaggca
    27601 gcaacaatga tccaaaacca agaattcaat tttttctgca cggatgtcca agtacgtgga
    27661 gaataccctg catacacagc gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta
    27721 gagcaaacgg aaaaagactt ccagttgcta aaagaatatc cagtggacta catcggattt
    27781 agttattata tgtctacggc aatcaatgag actgatccac aagcagcaac ttcagaaggg
    27841 aacttgctgg gaggagtaaa aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc
    27901 gatccagaag ggctgagaat cgcattgaat gaattataca atcgttacca gaaaccatta
    27961 ttcattgttg aaaacggtct tggagccatt gatcatatgg aagaggatgg cacgattatt
    28021 gatgattatc ggatcgatta tttacgtcgt catatcgaag cgatggctaa tgcagtagca
    28081 gacggtgtag atttgatggg ctatacgcct tggggatgta tcgatcttgt cagtgcatca
    28141 acaggagaaa tgagtaaacg ttacggcttt atctatgtgg atctagacga tgaaggaaat
    28201 gggacactga atcgatcaaa gaaaaaatct tttgattggt ataaagaagt aattgcctca
    28261 aatggcgtaa acttgtaatt aaagcagctg aatgaaaaag aacacacttc tcacatgtga
    28321 tttgtgttct ttttttattt tttatgtgct attcttttag taatagtaaa gcaaagggtg
    28381 acgttatgaa aaggacacgt gtactatact tggaagtcgc agataaaatt aaagaagata
    28441 ttttctcagg aaaatatcca gtcggttcga tgttgccgac agaaagtgaa ttagaagaac
    28501 tgtttcaagt cagcaaaatc actgtccgaa aggcaatcga actgctggca tcagatgaat
    28561 atgtcgcaaa gaaaagtggt cgtgggacga cggttctaag caatcgtcct tacaatcgat
    28621 tatccaaagc ggcaagcttt acgcaaatct tggagaactc ccgatatgaa gtccggaaag
    28681 aaacgctgga tttgcaattg gtccatataa agaaggaaga tcctttgttt tcctttttcg
    28741 gtgagcaagc tgtttgtttt aagcgcttat attatttgaa caatcagcct tatatttatt
    28801 ttgttcatta tcttccacaa gaaatgatgc aattcagcag agaagaattt ggcaaagaat
    28861 ctttgtatcg tttgctgacg aaaaaaggat atactattga ctcattttct gatgatttct
    28921 cggcagtatt tttaacagaa gaagaacaag gaattctgaa aacagacgca cagatcggca
    28981 tcaaacggat acggaaatcg tctacgcaaa ttggggaaat gattgaatat tcggaggcgg
    29041 tctaccatac ggagctgcat ccttaccata ttgattatga aacataaata aaaaaagagg
    29101 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    29161 atttttcgtt tggttaggct tattaccgca gatacacggc tgtttctgga ttaccattta
    29221 ttctggataa gggggtgtgg cacgattttc agaaggaatc tatgaaccaa tcactgtatt
    29281 gattttagct tcgatggtaa ccccattcgg cttgacgatc gcttatttct tagggaaaat
    29341 cattcgtaaa aatatactca atcgtcaaga aatcgataca ttgaagaccg cttttcctat
    29401 ggggatctgt cagatcacag agggatgttt ccctatcgta ttgaacgact tggcacgcaa
    29461 tgtcatagca acgggagtcg gtgggacagt aggtggcgga ttaagcatgt tttggggcgc
    29521 agatagccgt attccggcat cgggaatgtt tgcagtcgct acgatgacca gaccatgggc
    29581 attcattggt gcattgttag ctggttcttt tgtcacagga ataaccattt tattattgaa
    29641 aaaaccagta gatccgaatg cagaaatcat ccaagaagaa aaagaggaag aagacatatc
    29701 ttgggatgat ttgacaatta gttaggagag gcagaatgga ttacaagaaa aaagtacaat
    29761 agatgtacct tgagacagga atggtatgaa agaatcagag ttagaaagaa tcgaatatac
    29821 agattttggt ttaggagcga tcgaaactgt ccagagcatc ggcaccctcc ttgaaatgac
    29881 gatgaaggaa aaatgagaac ctttcgttgt cgaaagggtc tggtatacct tatgtcgaag
    29941 gaaaggaaac aaaaaaactt tcctgtacta ttccaaaaag gaaagaaagt tattatacag
    30001 taaaaaaaga aattattctt catgctagag atcagtacac gttcgagccc aatatcaaac
    30061 actagttcca ggcaggcgca acaggtgcgg ttatcactga attttcttcc agcagtgacg
    30121 attcaagtga tatttttaca aatccagcta tttatcgtta acaaaaagat ttttaggaga
    30181 agctgatgaa caaaaatgat caaattttga tcgtttagtt cgtcggcttt tttatttaga
    30241 gaaacatcat ttatcagtga aacataacac gagaacaaaa tgattttttc gttaataaaa
    30301 gaaacaagta tttgttgttt aaaataatac acgttattgg catataggac gaaatcccag
    30361 tatcttctat aagtaagtca tttcatatcg atgaaaacac gttatttatt tttgttttat
    30421 ctataaaatg gctgaaaaat gatttttttc taaatactat atatagtggc gtctatccta
    30481 tataccacta aaaaactaca aaatctagtc ttttatttta aaaaaacacg tgtagaatga
    30541 agatagactt ttgagttagg gagtgttttt ttatgaaact tactgttttt tgcggttccc
    30601 gttttggtaa taaagaaagt tacaaattta ttgcgcaaac gttaggaaaa tacatggcac
    30661 aagaagaaat cgaactggtt tacggtggtt catactccgg gattatggga gtgattagta
    30721 aaacagtact ggaaaataac ggaaaagtca caggtatcta tccgaatggg ctttttgaaa
    30781 ctgaattgcc tggaaaagat gttactacat ttattcctac agaaacgatc gatgaaagaa
    30841 aggtattatt atttgaaaag ggagatgctg tcctcgtttt ccctggaggt ctaggtacat
    30901 tggaggaatt ctcacaactt ctttcttgga tagcaatcgg tctgacgccc gacaagccaa
    30961 tcggaatatt gaatattggc ggttattaca gcggcttgca acaattatta gaaacttttg
    31021 caaaagagga ttttatggat aaaaaatggt tggatcaagt aatcttttcc aacaatccat
    31081 tggaactggt caatgaatta cgagcagtat ctattaaaaa tcagttatta ttgaaggagg
    31141 caaaataatg agtcaaatat atttagcagg accatttttt tcagaggagc aaatcgatcg
    31201 agtaagtcgt atcgaaaaag cgttggagga aaacaaaaca gtcacaagtt tctactctcc
    31261 acgtcatcac caagaaagca attatgaact attcagcgct ggatgggcgc aggaagtata
    31321 cgaaaaagat atggaagaac tgacaaatgc agaatttgtt gttgctatcc ttgactttga
    31381 gcatcaaaca atcgatccag gaacggctta cgaattaggt gtggcaacta tgttgaaaaa
    31441 accgatgatc attgtacaag aagagacggt acctacgaac ttgatgatta cacaaagtct
    31501 ccatacgtat ttaaaatcag accaagctgt acgtgaatat gattttgaaa cattgcctgt
    31561 tgaaacttac gtaggcgagt acttatagaa gaggaagggt tgtatgtcag tgattttgat
    31621 ttcaggaacg attggtgcag gtaagagcag tttgacggat atgcttgcaa aagagattga
    31681 ttctaaacca ttttatgaaa atgtcgaaga taacgaagta ttgccgctct tttattcaaa
    31741 tcctgagcag tacgcttttt tattgcaaat cttctttttg aacaagcgat ttttagcaat
    31801 gaaaaatgct ttagtgaacg atgacaatgt gctagaccga tccatctatg aagatagcct
    31861 gttgttccat ctaaacgctg atttaggtcg ggtaacggat atcgaagtac agcagtacga
    31921 tagcttatta gatacgatgc taaacgagtt agatgatgtt gctccaaaaa aacggcctga
    31981 tttgatggtc catatcaaag tttctttgga cacgatgcta gaacgtatca aaaaacgtgg
    32041 gcgtgactat gaacagctag aaagcgatga gacgttatat acctattatg aaacgttgaa
    32101 cacccgctat aatcaatggt atgaagattt tgatgtatgt cctaaaatac aagtggatgg
    32161 ggataaattc gattttgtag aaaatccaga agatgcaaaa aaggtgatcc gtcagatcca
    32221 agaaaaatta gctgaattgg aaggaaagcc tgctggaaaa tattttacgg ataaccagca
    32281 tgcaaaaggt caagaattag gctaaaaata aaaaaccaaa cgacgggaaa gaaaaattac
    32341 tacgtgatgt agaataagcc tttctatcgt ttggttattt tatattcaaa tatttgaaat
    32401 aaataaatct ttactctttg atttgtaaac tgaaatgatc cactgtttgt tttactgatg
    32461 aagcttgatc aaaggaagaa gctgagtttg aagttcataa aaatcagcca tagtgagttt
    32521 tgcttggatt tgtttatctt cttctacttt ttacataatt ttgaacagga taagattcaa
    32581 atgttttaaa tgaatctttg actagtttgt gacagttgtc gcatatgctc aagcttcctt
    32641 ggtcattaaa ttctgtatgg atatttttgt tgttattaca tgtacaataa tcaggactat
    32701 cgatcatcca tttcgtctcc tttacaatca tcttgcttct ttactatagc agaaaagaag
    32761 gggagaaaca aaagaagatc caacagacgg cactatagac tgtagatgtt gccatcctca
    32821 tccttcaaac ggtaattacc gatttttgta aatatgactg ttgtttcttt tttattttct
    32881 tttcctgaaa taatatattc gtcatctttg gtagtgatct tgatatctgt cagcactgtt
    32941 ttttcttcag atgattcaga agaatcagtt ccttgtgtct gatacgtgcg tgtccattcc
    33001 atttttttat ctgtaaatgt caactgtccc gcggataaat ccatatcttt acgtactttt
    33061 agcgtatata cactgttggt ggtatctgct ttttccgctt ttaagacaga agaatcggtt
    33121 gtttcgtccg aggattggtc agagccgcca aacaaatcac agccagtcaa tgtcaaaagt
    33181 cccgcagaca aaataaatag tacgattaat ttcttcaaaa tacttccccc atttgatagg
    33241 ataaagaaat tataccattg gaatgtaaaa cttcaaacgg atggaagaga aaaacgcaca
    33301 aaacacccac aaatagagta aattttagaa taaaatttta aaaaggcaaa aaatccgcca
    33361 ctttcagaaa gcgttttaaa aaagtgttgc aatcttttaa aaaaatgcta tattaaaaat
    33421 gaaccggttc aaatacttgt ttaagtataa ttaatagtga gaggaagttg ctctactgaa
    33481 gactttagtt atatggatat tgatgacaag caacactatg caaagaatgc ggtcatcata
    33541 atcgaacaac aagaaagaga aatcaaatgt aactggcagg agtgagaatg tatggcatcg
    33601 atccgtgatg tggctaagat ggctggtgtt tcagtgggaa cagtttcccg ttacttgaat
    33661 ggtcagcaac tgaaggaaaa gaacatgaaa aaaatagcag aagcgatttc agcgttggac
    33721 tataaagaaa acatcattgc taaagggttg aagaataatc gaagcttctc tattggactt
    33781 ttgataaacg ggatttccag ccgttttggg tcggaagttg tctcagggat cgaaagagta
    33841 gctgaagaaa atgggtatag cttattatta agcgggtttg ccgatcagcc agaaaaaatt
    33901 gaacaaaaaa ttgaatacct gatgaagcat gtgatcgatg ggctgattgt cttcttatcc
    33961 gaagaagagt ggtctggatt tgaaatgctc acaaaaatga gtattccagt tatcgcattg
    34021 aattgtccag aaggaccagc aggtattgat acaatattag tgaatgatcg agaaagtgta
    34081 gcaaaagtga tcgctcatca tggtgaacaa ggacataaaa aaatcggatt tattgctgcc
    34141 actcaaacag actacgtagc aagagaacgt ttagcaggag ccaaagaagc ggtccaaaat
    34201 gatccatcga tccaattaga ggtctttaca ggagactatt ctagaaaaag cggctattac
    34261 ggggcaaaag cattattaga aaaaggggca actgctattt ttgtcagcaa ttacaatatg
    34321 tcgattggtg ccatcgaatg tttcaatcaa gaaggtgtca gaatcgggaa ggatattgcg
    34381 ttcagtcact atgattattt agataaaaac gatctgagta tccttccgaa aatcacgatc
    34441 acgccaccaa cagataaaat cggggaacga gcagcagaac agctgttaaa acgaatcaaa
    34501 aaagaagagg aagaaaatga aaaagtgatt gtgatggaaa atatcataca tggaatcaac
    34561 taaaacgaac taaaaaagaa aaagaggtgc tttgatgaaa aaatatcaat ttccagcaaa
    34621 tttttggtgg ggttctgcag caagtggccc acaaacagaa ggaaggattt caggcgatgg
    34681 aaaaggagag aacatttttg attattggta caaaaaagaa ccgcaaaagt tctttgatca
    34741 agtaggacct gaaaaaacct cgcaagttta tacgaagtat caagaggatg tacagttgat
    34801 gaaacagact gggcataatt catttcgaac atctatccaa tggagccgtt tgatacctga
    34861 tgaatctgga acaatcaacg aagaagccgt tcgtttctat catcgttatt ttgatgagtt
    34921 gattgaaaac ggtatcgaac cctttgtgaa tctgtatcat ttcgatatgc cgatgtatct
    34981 gcaagaaaaa ggcggatggt tgaaccgaga aacggttgaa gcctatgaaa aatatgccaa
    35041 aagttgtttt gaattgtttg gttcgaaagt aaaaaaatgg tttactcata atgaaccaat
    35101 cgttccagtc gaagcaggtt atttatatcg atggcattat ccagaagagt cagatatgaa
    35161 aaaagcgatc caagttggct atcatgaagc gttggcttca gcgttagcca tcaaatctta
    35221 ccatgaaatg gagcaaggtg gtgttattgg tatcatcttg aatctgacac caagctatcc
    35281 aagagacgaa tcaaaccctg aagatgttaa agcagcgcaa atagcagatg cgttcttcaa
    35341 tcgttcattc cttgatccag ctgtaaaagg cgagtttcca agtgaattga ttgagatagt
    35401 aaaagaactt gatctcatgc ctgtgatcca tgcagaggat ttgacggtta tcaaagaaaa
    35461 tacagtagat cttttaggta tcaattatta ccagccacgc cgggtaaaag caaaagaaac
    35521 accaatcgat actaaaaacg gtccaatgcc tgaggattat ttcgacaatt atgaaatgcc
    35581 tggtcgtaaa atgaatcctt atcgcggatg ggagatctat gaacaaggaa tctatgatat
    35641 tttgatcaat gtacgagatc attatggaaa tatcgactgc tttatttctg aaaacggtat
    35701 gggtgtagaa aacgaagcac gatttatcaa agaagatggc atgatccacg atgattatcg
    35761 gatcgaattt gtccaatctc atctgaaata tgtccatcaa gccattcaag aaggtgccaa
    35821 ttgtttaggc tatcatatgt ggacatgtat ggataactgg tcgtggacca atgcatataa
    35881 gaatcgatat ggctttattt cagtagactt agcacaagat ggaaaaagaa caatcaaaaa
    35941 atcgggtcat tggttcaaag acgtatcaga taaaaacgga tttgaggctt aagaaagaat
    36001 agttgagagc aaatgcgtga aattctccat tcttaccatt ttgtgaaaaa gaagttcaat
    36061 tatttacgat ctttttcaac ttttttgtct ttctatttca ttttttgtat ataatagaat
    36121 agtcaaaatt aaataggagt ggaggaaagc atgaggaaaa aaagaccgtt tcttctcttt
    36181 tggttattcg gttttttctt actgcttatt ttatccgcag cattttcagg aaaaatgaca
    36241 gcagatcttg ctcagaaaga aacagaaaaa atcgtagcaa gcaaagaatc agtagataat
    36301 ttcaaaaaag aattctcaac ttattggtgg gcggagtata aaataactgc caattacgaa
    36361 aaaggcgaat tggacattta tttgccaaaa ggaaaagaag aaaatcaatt tattaatatc
    36421 gatgacgtac ttagtgcgat ttctctcaga caatatcaat ttaaagacaa gcatttgacc
    36481 gttttgcttt tgactcatga tggcgacaaa gtagcccgtt tgaaaaatgg aaaagtgatc
    36541 accgcttatt ctgaaaaagt agacaaaaaa gtggtcgccg atcgtttagc ggaatggaaa
    36601 aagacaaatg aacaagtcaa gaaaacaact gtctcttctc aaaaaaacga gtcgacagga
    36661 acaagtcctt cagattctgg cagtggacaa acgataacgg gaagtgaaga aaatcaagcc
    36721 tcaattatta cgaatgagtc atcacaagga caatatcaat cctatggaca atgaaaaaca
    36781 gaggttgtga aatcagcgtt ttgacctgag tattagagaa acaaacggca aaatagttcc
    36841 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtgtatca
    36901 agaggtcttg agcctgccgt ttaactctga gccacaagga gcaatttcaa aaaatagctc
    36961 cttatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta
    37021 ccaagaggtt gtaaaattag cgtcctgaca taatctaata ccgagggtca gctgattgaa
    37081 caccgtgtat caagaggtct tgagcctgcc gtttaactct gagccacaag gaacaatttc
    37141 aaaaaacagc ttctcatgtt ttataaatat tgtgacttgt ggccgaggag ttggcaggag
    37201 aacaccgttt aaggaagaag ttgtgaaaaa gctgtcctgc atcaagaaat aaggagctgc
    37261 gacaagaatt ttgtcacagc ctcttttttt gtacgaatac cgagaaactg gcttttgaat
    37321 accgtggagt gaagcggtca ccacaagact tttatctctt cattagttta atcgcttaca
    37381 tcgcagcggt tttatgctgt gaaaagtgga cggaataatc tttagaaaga ataaaaaaat
    37441 cttaagataa caactttttt aggaaatttt catttttcag gagcaaaata agaaaaaatg
    37501 tgaatagaga gaagtgatat acgtgacaca acttaaaaaa ttgtgccaaa atcgtttagc
    37561 aatcgttttg acagctgttt tctttttctg gctgaagacg atcattgcct actatgcaga
    37621 tttttcatta ggtgtggaag gtacgatcca gtactttatc ttgtggatca acccaattgc
    37681 gactacctta ttgttttttg gcctttcttt gtatattaaa aagccaaagc cagcgttagc
    37741 tattcttttg atcattgata ttttaaatac gcttttgttg tacttgaata ttatttttta
    37801 tcgggaattc acagatttca ttacagtcaa atctgtatta ggcttttcga aagtgtcgca
    37861 aggattgtca ggaagctcct tttctttgat gaagccccat gatgtcatct actggctgga
    37921 tatcgctgtg ttcattgggc tactggtttg gttgaaggtc aagaaaatac ctatcaaaag
    37981 caaccctgtc ggcaaaccga tggctatcgc tgttacctgt ctttcagggc ttattttttc
    38041 aggaaatcta gcgctgagcg aagccaaccg tccacaattg ctgcagcgaa catttgaccg
    38101 ttcatatatt gtcaaatatc taggaatcga tgcgtatact atttatgacg gaatcaaaac
    38161 aggaatgact agcagtgttc gcgcgcatgc aagcagtaat gggattgatg aagttttaga
    38221 ttatacaaag aaacattatg cagagccaaa tcccgaaaca tttgggatcg ctaaagggaa
    38281 aaatgtgatc gttctccatt tagaaagttt ccagcagttt ttgattaata tgaaggtaga
    38341 cggacaagaa gtcacaccgt ttttaaatag tattttccag aatcaagcaa cgatcagctt
    38401 tgacaatttc tttcatgaag taggacaggg aaagacaagt gatgctgaaa atatgttgga
    38461 aactggcaca tttggtttac ctcaaggctc attattcaca gaacttgggt cagacaatgt
    38521 ctttcaagcc gcaccagcga tccttggaca aaagcaaggc tatacaagcg ctgttttcca
    38581 tgggaatgta gctagttttt ggaatagaga tcatgtatat aaaaacttag gttatgacaa
    38641 tttctttgat cgatcttatt ttgatgaatc cgacgaaacg ttaggttatg ggatattaga
    38701 taaagattta ttcagagaat ctgcccaata tcttgagcat ctacaacagc cgttttacac
    38761 gaaatttctt tccgtaacca accacacgcc ttattatacg gatgacaaga atttcgactt
    38821 tccatctttg aatactggca atagcacagt agataattat gttcgaacgg cacattatct
    38881 ggatcaatca ttggaacaat tttttacgta tttgaaaaaa tctggtattt accaaaattc
    38941 aatctttgtg atttacgggg atcattttgg gatctcgaat acagataata aagatttagc
    39001 aagcgctctt ggtaaagatc cagatacatg ggatgaattt gacaacgcac agatgcaacg
    39061 agtgccattg atgatccata tgcctggtta tactaaaggg acagtcaatc atgaatacgg
    39121 tggagaaatc gatgttttgc cgacattact acatctattg ggaatcgatg ataaagatta
    39181 tcttcatttt ggtacagact tattatcttc tcagcatgat caagtagttg cttttcgaaa
    39241 tgggaacttt gtgacaccca aatacaccgt cctaggagga aaagcttaca ataatcagac
    39301 aggagaaatc atcgatacga aagcagcagg aatcgatgag gaaatctcca aagatcagga
    39361 aaaagtcaag actgcgttaa gtctttcaga taagctgaat caggaaaatc tcttgcggtt
    39421 ttatgtgcca aacggatttg agacgattga tccgaaaaaa tatgactata aaaatgaagc
    39481 ggaacgtaat gaagcaatcg aaaagcaaaa aggcgaacat tcgacaagtg tcttttcaaa
    39541 aaatggtaat aaaacaacaa ccaacttata tccagtaaca agaacagacg ttgaacagca
    39601 aaaaaataat caataaacaa tcaaaccaac cttcagtagt tagacaacat tctaatgctt
    39661 gaaggttggt attttttatg aaaaaaacat ttttttattt ttttcgtgta tttttccttt
    39721 ttaacgtatc tatttattga atgtggaaaa atttaaatta aaaaggagaa ctattatgta
    39781 tgatggtgaa ttgatcaaga aacttcgagt caacaagaag ttgacacagt cccaattagc
    39841 ggaaggaatc tgttttaaga cttctctagt cgggattgag acaaattcag tgaaaaaaat
    39901 gtcatttgta acattgagat catttttaga gcgaatgaac atgacacttg cagaatatga
    39961 atggatgcga aaccagctgg atgaaccaat aaagttaaag aaaaaaagac gaatgctaga
    40021 tcgggtacaa gaagacagct ttgatcctta taaagagatc agcaataacc gtaggcattt
    40081 taagaaaaca gaagatctgt attatttggt tttgaatctt cacatggtcg aaaaaacaga
    40141 agtatctgag acagaatttg aatttttacg ttatgaatgc cgaatcattg aagaatactt
    40201 gatgcagata ccagactatg gtcaatttga actatctatt ctagctgatt ttccttggat
    40261 attcagtgat cggttcattg agaacaatta tttgaaaatc aaaaaaagaa tgcgacgatt
    40321 agcacagttg aacaatcatg agcagtatct ctttactttt ttaatgaacc tgacttcgtt
    40381 ttatatagaa aatggtcggt tgaaaaaggc acgtagtgtg aatgaagata tgaaacggag
    40441 cttagcacat aaagaaagaa gtacagtgat ctatgaaaca ttgatggcag attattatca
    40501 aagattgatt tctgctgctt taggggaaga aaagacgaaa gactaccaag aattcttcac
    40561 agtgctcgaa tatatgttag gaaagaatca acggaggtcc ttagagaaaa aactgctggc
    40621 cattgctgaa cccagcaact agaccagcag tttagaattt gtatcacttc gccgcttttt
    40681 cagacggttt gtttatatcg aatggcggga aaaagcgggc gatctctttt accgcagcat
    40741 cacgggaatc agaagcgtga atgatgtttt cagtccccgg cagcgcgtag tctcctcgga
    40801 tcgtacctgg aacagcatcg gcagcttttg ttgctccaac catcttacgg acgatatcga
    40861 tcacttcctc gccgaccagc acaagataaa cgacaggacc ggaagtcatg taatcgatca
    40921 gttcatcaaa aaaactgcgc tcagtcaagt gctgataatg ttcttttgct aattcaggag
    40981 tcatagtttc aaacttcatt tcagcaatgg ccagtccctt tgcttcaaaa cgttggatga
    41041 tgcttccgac taaatggcgt ctcacgccgt ctggcttgat aataacaagt gttctttcca
    41101 taacagcacc tcgtttccta ctatatttct attatagcgg gaggtggaaa atgagacaaa
    41161 taaaaccgcg tgagacagga atataacgtg tgtattttaa aaaaagtatg aaaaacatgg
    41221 aaaattgatt tgacatcctc ttttttagtt gttatattaa cgaaggaaaa ccgaataaga
    41281 tttgttaggt gaggctccta tacaaacata ggctactgcc caaaaacgtc gagagacgcc
    41341 aatgggtaga acaggaattg tcgagataag gcttttcata aggtagctaa aagcaggtat
    41401 ctgcttttta cgttgtacag tgtcaaaact caacgatgag atctgagaag ctttttttaa
    41461 tatgcttttt caaacctttc gctgagtatt tggcgagagg tttttttgtt gccgcaaatc
    41521 cccatcggtg aaaaataaaa aggaggtggt ctaattggaa aaataactat tcgaaaaatg
    41581 attggaagtc atgaattaaa attacttagt aaaggagatg agaacaaatg gatgacagtc
    41641 attcgaaaaa caaggatcta gaggaaaggc cagggctatt atctatctgt tctcagcccc
    41701 tttcagtttt ttctctatcg atccgatgaa tagctaaaga ccaaaagcag cgagaacagc
    41761 tgtcttttgg acacaaagga gagattgaga tggaaaaaaa gaaagataca caaaaaatct
    41821 atgcagcttt tgctggaaaa acgaaacaag aattatttga actattccat gcatcagcaa
    41881 atggattgac agaagaacaa atcgaacgtt caagagaaaa ttacggtgaa aatgcgattt
    41941 catatggcaa aaaaacacca ttcattgtag aaatattgaa agcatacatc acgccattta
    42001 cattagtgtt gatcgcatta ggaattattt cattcatcac ggaatatgta ttagcagctc
    42061 ctggagataa agatcttttc ggtgtagcca ttatcttcac tatggttatc gtcagcggga
    42121 caatgacgtt gattcagtcc gtacgttcca atcaagcggc tgaaaaactg aaatcacttg
    42181 tgaaagtaac ggcagctgta aaacgtaatg gaaagtacac agagattcct atggaagaaa
    42241 tcgtctgtgg agatcttgtt cggctttcag caggggatat gataccagcg gatatgcgtt
    42301 tattgtccgc aaaggattta ttcatttccc aagcagcatt gacaggggaa agctatccag
    42361 tagaaaagca tgcgaaagta tgtgaagacg atcagcaaag cgaaacaagt tacgaaaatc
    42421 ttgtctttat gggaagtaat gtcatcagtg gaagtgcgga aggaatcatt gtttctgttg
    42481 gaaatgaaac actatttggt catgtagcag aaacgctatc tgaaaaacca attcaatcaa
    42541 gctttgaatt aggcatccat aaaacatcca tgctactgat caaatttatg tcattgatgg
    42601 ctccagcagt tatcgtcatc aatgggctta caaaagggga ttggctagaa gcttttcttt
    42661 ttggactatc tgtagcagtt ggtctaacac ctgaaatgct tccaatgatc gttacgacga
    42721 atttggtcaa aggagcaagt gtgatggcca aaaaaggaac agtgatcaaa aatttgaatg
    42781 ccatccagaa ctttggtgca atcgatgtgt tatgtacgga taaaacgggg acattgacac
    42841 aagataaaat cattcttgaa tatcatatgg attgtgaagg aaatgaagat gatcgtgttt
    42901 tgcgccatgc gtatctaaat agctactatc aaacagggct gaaaaatttg ttagatgtgg
    42961 ctatcattga tgaagcaaca gaaactttag atacagataa gatcaattat caaaaagtgg
    43021 atgaaattcc ttttgatttt gaacgtcgtc gtatgagtgt tgtggttgct gatccagctg
    43081 gtaaaacaca aatgatcaca aaaggcgcga ttgaagaaat gctgaacatt tctaaattta
    43141 ttgatcttgg cggtactgtt acaccattaa caaaagaacg aaaagaagcc gtacttgcaa
    43201 aagtacagtc acttaatgaa gatggcttac gagtgattgg tgtcgcacaa aagaccaatc
    43261 caagcgtcgt tggagaattt tcagtcaaag atgagtcaga catggttttg attggttatc
    43321 ttgctttcct tgatccacca aaagaaacaa cgaaacaagc gttgaaagct ctgaaagaac
    43381 atggtgtagc tgtaaaagtt ctgactgggg acaacgaatt agttacacgt tcagtgtgcc
    43441 gtcaagtagg tctagagatc aatgaattgg ttaccggtga gaaaatctcg gaaatgagtg
    43501 atcatgaatt agcacaagta gctgaagacc atgaagtatt cgtaaaattg aatcctcagc
    43561 aaaaagcacg tttgacgaca gctttacgta aaaatggtca tactgtcgga tttttaggag
    43621 atggtatcaa tgacgctcct gcaatgaaag tggctgatat cggtatttct gtagatacag
    43681 ctgtggatat tgcgaaagaa tctgcagacg tgatcctttt agaaaaagat ttgacgattt
    43741 tggaaagagg ccttctttct ggacgagaaa cttttgggaa tatcatgaaa tatatcaagg
    43801 ctacagcaag ttctaatttt ggcaatatgt tttccgtgct gattgcaagt acgttcctgc
    43861 catttttacc aatgctgcct ttacaacttt tgtttttgaa tctgatctat gatgtttcct
    43921 gcatttcctt gccatgggat cggatggata aagaatattt ggacgagcca aaaaaatgga
    43981 atgcatctag tattggggaa ttcatggttt actttggtcc aacaagttcg atatttgata
    44041 ttactactta cttgttgatg tattttgtta tttgtcctgc tgtagtgggc ggaagtttcc
    44101 atacattgga tagttcacag cgagtgattt tcatcgcatt attccatgca ggatggtttg
    44161 tcgaatcact atggacacaa accttagtgc tccatgcact gcgtacaccg aaagtaccat
    44221 ttatccaaag caacgcaaca tttgccatgt ttgttatcac tacattagga attattgtcg
    44281 gttctgtcct tccttttaca agttttggtg ctgaattagg cttgatgcca cttcctggca
    44341 attactggtt atggttattt gctacagtca tcgcttatct ggcgcttgtg acagctgtga
    44401 agaaaatcta tatccgtcga ttccaagaac tcttataaac gagaaggctg gttctttgct
    44461 tctgttcttt ttggaccagt cgtgctaaac tatgtgtatt gttttggaaa ggattaatgt
    44521 atagatgaaa ggtgcaatat ttgatttaga tggtttgtta gttgacacag aaaaaacgta
    44581 tcgtgatggc tggctttggg gatttcagca atttgatatc catatcccaa aagaaatcgt
    44641 tgacgcttgg ggcggaaaaa actggaaaca aagttttgat attttagtga aagcagcagg
    44701 tagccctgaa aaagtacagg aagtccgagc gaagcgtgaa gaatattttt atcaacagct
    44761 gacaagcgga gccatccact taaaaccata tgcaaaagaa atattggcag acttaaaaga
    44821 acgtcatatg aaactaggca ttgctacgac cactgttacg aaaagagcaa cagatatctt
    44881 ggatcacttt aaattagctg attacttgga cacgtttaca ttcggagacg aagtgtcgga
    44941 gaacaagcct tcaccgattc cttatctgac agctttggaa cgaacaggat tagaagctag
    45001 ttcagccttt gcagtggaag attctttagt aggagcaaca gctgcttcac gagcaggatt
    45061 aggcgttgtt ttgatacctg attccagttt tgtccgaaat tataccgtcg aagaaaagga
    45121 aaaattgaaa cttcttgcgg aaggaaaaga tcttcgaact gttattgaaa tgttaaataa
    45181 aaaaacagaa aaataaatat agaaaaaata atttgaattt cgtacttgac ttttcagttt
    45241 tcctcatgta gattaatccc aacaacaaaa tatcgatttc gttgaaaagg aaagtaagtt
    45301 tggaaacttt gcagagaggc tctggctggt gagaagagtc aagtaagaaa agctgaaaat
    45361 ggccttggag aataatttgt cgataagtag gcaaattcgg aactgttcga ccgttaaaac
    45421 gaacgccaat ctctcttttt gagatgttgg gcttgaggga aggaaacttc cgaaaaaagg
    45481 tggtaccgcg atacgtcgtc cttttactga catagtttat gttagtaaaa ggactttttt
    45541 tttgaatatt atttgcattg aaatggtaag tacgatagaa gaacctttga cagagagctt
    45601 cggtagctga gaagaagcaa ggaacgacta ttggaaaatg gccatcgagc aattcatccc
    45661 gagcatagat gtaaggcgat gacggctgcg accgatatct tgctagagta tgatcgtact
    45721 tgaagaggag ttgtgtgtga acacagcttg aacatgaggt ggtaacacgc aaaaaagcgt
    45781 cctcaagaaa atgacatttt cattttcttg aggacgcttt ttttagataa ggaggaattt
    45841 taatgattga attgcgagat gtttctgtat gtttcaaaca aaaagaaaaa gagatacagg
    45901 cagtagattc tgtagattta acgattgaaa aaggcgatgt ttatggaatc gtcggttact
    45961 caggtgcagg gaaaagtacg ttagttcgtg tgatgaattt gctgcagaaa ccaacagcag
    46021 gagaagtcat tgtcaatcag acgaatctga gtcagctctc tcccaaagaa ttgcgaaaag
    46081 aaagaaaggc catcggtatg atctttcagc atttcaatct aatggaaagc cgaactattt
    46141 ttgacaacgt tgatttttct ttaaagtatt ctggaaaatc gaaacaggaa cgtcgtcaaa
    46201 aagtgaatga actactggaa cttgtcggtt tggaagaaaa agcatctgct tatcctaaac
    46261 aactgtcggg gggacaaaaa caacgagtag caatcgcacg agctttggcg aatgaaccta
    46321 aggtattatt atgtgatgaa gcaaccagtg cacttgatcc gaaaacgacc cttcagatcc
    46381 ttgcattgtt aaaaaaattg aatcgccagt taggtttaac aatcgtcctg atcactcatg
    46441 agatgcaagt tgtcaaagaa atctgcaaca aggtagcggt tatggaagat ggaagaatcg
    46501 ttgaaaaagg tagcagcatc cagatcttca gcaatcctga agaagaattg accaaagatt
    46561 ttattcgaac agctactcat cttgaccaag cattagaaac gatcattgcc cattctgctt
    46621 ttgccgatca gatcgccaat aaatggctgg tggaattaag ttatattgga aatcagacga
    46681 acgaacctct aatcgctcat ctgtacagca aatatcaagt aacagcgaat attttatatg
    46741 gcaatgtaga gttattacaa gaaacgccgt tagggagttt gatcgttact ttagctggag
    46801 aaaccaaaca gcggaaaaaa gcattggatt atttgatcaa atctggtgta aaaataaata
    46861 ttttacaaaa aaacgattta gatagtcgta tcaaagtgat cgaaggagga agataaaatg
    46921 actgatttct ttaaaacata tttgcccaat gtttatttga ttccggatga atttgtcgaa
    46981 gcgacgaagc aaacaatata tatgtctttt tggacagcat tcattggcgg gatgattgga
    47041 atcatcttag gtgtgacgtt agtagtgaca cgccctaatg gattactagc taaccgtctg
    47101 ctatttgaaa tacttgataa gctgatcaat atcattcgtt ccatcccttt cattatcttg
    47161 ctttctcttt tagcattgac tactcgtttc ttagttggaa cgacgattgg tgcgaaagca
    47221 gctttagtgc cattgatttt tggagttgtt ccgttttttg ctcgtcaaat cgagaacgca
    47281 ctgctagaag ttgatcctgg ggtgatcgaa gcggcagaag cgatgggaac cagtccaatc
    47341 ggtatcattt tacgtgtgta tttgttagaa ggtttgcctg gtatcattcg ggtctcggcg
    47401 ttgactatca tcaacgtgat cggactgact accatggctg gagcagtagg tgcaggcgga
    47461 ttagggaatc ttgctatatc gagagggtat aatcgattcc aaacagatgt aacgattgtg
    47521 gctacattat tgattctaat cttagttttt ttaagtcaat ttatcagtaa tcaactgatt
    47581 aaaaaaatta cacattgaaa gtggaggaga aaaaatgaaa aaaattattc gttttgtagt
    47641 cattacattt gtagcaatta tttggttggc agggtgtagc caaggaaaag cagatacaac
    47701 tgacaaaaaa gaaactatcg taaaactagg gattattgga gaagatacag atgtatggga
    47761 caacgtgaaa gaccgtttga aaaaggagaa cattaatttg gaatacgtga aatttacaga
    47821 ttacaatcaa ccaaatgtgg ctttagcaga tggatcgatt gatttgaatt ctttccaaca
    47881 tcaattcttt ttagacaatt acaataaaga acacggaact gatttagtat cgatcggaaa
    47941 tactgtcaat gcaccgctgg gaatttattc taacactttg aaggatgtag ctgatatcaa
    48001 agacggagca aaagtagcaa tccctaatga tgttacaaat ggcggcagag ctcttctgtt
    48061 attgcaaact gctggattga tcaaagtgga taaggcaaaa ggacaagcgc ccactgtttc
    48121 tgatattacc gagaatagaa aaaatctaga catttctgaa ttagatgctt cccaaacagc
    48181 acgtgctttg tcagatgtag acatttcagt gatcaatagc ggtgtagctg tagatgctgg
    48241 atttaatcct gcttctgatg ccatcttctt agaaccagtg gatgaccatg cacgtcctta
    48301 tgtgaatatc atcgttgcaa gaaaagaaga tgaaaacaat aaaacgtatc aaaaaatagt
    48361 agacgcttat caaacagaag acacagtcaa agtaatcgag aaaacatcaa aaggatcaag
    48421 cgtgcctgca tgggaaacat tcgggagaaa ataagaaaaa tgactagcat aggaggaatt
    48481 ttttatgaca acaacaatct caacaaacca attgaaggat ctaatccaat atcgtttacc
    48541 agaatatcaa gcacttgctc tagatatcca cgaacatcct gaagtaagta actatgagtt
    48601 ttattcctca gcagcgttga ttaaacaatt agaaaaagaa aggttttggg tagaaaaaga
    48661 agtagctgga caccgaacag cctttgatgc acgttatatt tccaataaac ccggtcccac
    48721 aattgccttt ttggcagaat tcgatgcttt accaggtatt ggtcatgcat gcgggcataa
    48781 tttgtttggt acttactccg tattagccgc cagcatagtg aagcaattca ttgatgaaac
    48841 tggtggagaa atccgtgtat atggcacccc aggggaagaa gggggcgaga acggatcagc
    48901 taaaggcagc tttgttcgag aaggtttttt tgaggatgtc gatgtagcgt tatgcgtcca
    48961 tccagctttt cgttatggga aaacgacaga atctttagcg aatgatccag tcgacatcaa
    49021 attttacggt gtcgcttccc atgcagcagc agcacctgaa aaaggagtaa atgctttgga
    49081 agcattgatc caagtgttta atggaatcaa cgctctacgc ttgcaattgc caaaagatgt
    49141 caatatccat gggatcatta cagacggagg agtagctgcc aacgtcattc cagattacgc
    49201 tgctggacgt ttctatttac gtgcaggaaa tcggcaaaca ttagataaag tatatgaaaa
    49261 agtggaaaat atcgtacgag gtgctgcgat aagtacgggc acgacttttg agtttggatt
    49321 gttccaaaat gcagttgatg atgtcatcgt tactccttct tttgatgact tattcttcca
    49381 gcatgcaaaa gaagcaggtg ttccagatga agaaatagaa acggaacaaa gaacaagtct
    49441 cggttcttct gatgtaggaa atgtcagcca agtcatacca acaatccagc ctacggtagc
    49501 aatctctgag acatatattg ccggacacac agaagaattc aaagctgctg ctaaaagtga
    49561 caaaggcctt gcttctattg cgattgcagc agaactactc gccaatacag ctcttgatct
    49621 gttgaaagag ccaaagctat tagaaagaat caagcaagaa catcaagaaa tagtagcaag
    49681 aaaaaaacaa gagggcgcta ctttctagta aaaaataaac ttgtgtgtga aagttataaa
    49741 aataattttc agaaaaatga aaaaatattt tctttttcat tgacaaatga tcttatggaa
    49801 ggtaaacttc gtacaagtca acttttcacg cagtagcggt taggtgtttt gttcagaaag
    49861 tcggtggtcg ctgtgaaccg atcaagccta attgacgaat tacatggaaa gtccatacat
    49921 ttgaataaaa gtttgaaagt gaaaaacaga tctgttttta attaaggtgg taccgcggag
    49981 tcattcgtcc ttagtttaaa acaggtcttt ttatttttat aaaggaggaa tcgcggatga
    50041 attgcggcgg agtaatccga tatcttaact tttttagaac ataagaacaa ctaaaaaagg
    50101 agagattatc atgggaaaaa aaggaacacc aagtttttta gaagcaacac ttgtattaat
    50161 ggttattgtt ttaggcattg cgattggcgt tgtcggatta aaggtctcac ctaatatcac
    50221 gatcttagca gcgatcggaa tggtcatgtt gtatgcagtg ataaaaagat acccgacaga
    50281 ttggctgcac gaaggaatca tcaatggcat caaaccagga atcatcccaa tctttatttt
    50341 tattttagtc ggttcattga ttgctgtatg gatacaagca gggatcattc caactctaat
    50401 ggtcattggg ttcaaattga tcagtgtcca gtggtttgtc ccttctgtat ttgttgtgtg
    50461 tgcaatcgta ggtagcgctg tagggagtgc ctttactgtt atgtcaacga ttgggattgc
    50521 atttttcggt attgggacaa cactaggctt aaatccagcg ctggttgttg gagctattgt
    50581 ttcaggtgcc gtttttggag ataagatgtc tccattatcc gaatcaacaa atctagcagc
    50641 agctattgtt gatgccgatt tgttcaaaca catcaagcat atgatgtggt caactgtccc
    50701 ggcatttgtt gtttctttct ttttattcat gattttagga cacacaagta aagccgcaag
    50761 tctttcatct attcgtgaag tgactgatgt tctagaagca aactttacta tttcaatctg
    50821 gtcactgatt cctttatttt taatgttact gtgtgcatgg aaaaaggtgc cggcaatttt
    50881 aacgattttg ttgaatatcg ctgtagcagt aggaatgatt tttattcaaa acccacaaac
    50941 ttccttaacg aagttggctg gggtcatcga atcagggttt gtttcaaata ctggaaacca
    51001 acaaatcgat tcactgctta gccgcggagg aatcgaaagt atgatgccta ctgtgtcatt
    51061 gattattcta actttgtcat taggtggatt gttgatcgaa tttggcttga tctcaaccgt
    51121 gatggaacga gtatcaagaa agatgacgaa cacaccaaat ttgattttta caacattgat
    51181 gacaagtatc ggagtaaata tttttattgg tgaacaattc ctatctgtta ttctgcctgg
    51241 taatgcattt aaagaaactt atgaaaaagc aggatttgat ccaactgtat taggtagaac
    51301 gctagaagat ggtggaacag taatcaacta cttagtgcct tggggaattg ccggaagttt
    51361 cgtagcaagt acatttggta ttcctacact cacttacttg ccattcgtat ttttcagttt
    51421 gctttctcct gtgttttcga tggtcagtgc cttcacaggt tggggaatct caaaaaaatt
    51481 tgtgaaaccg caagaagcag taagcaagac agcgcaatta gaacaataaa aacataaaaa
    51541 gggattgtga caagactttt gtcacaatcc cttttacgaa taaacggtat tcaaaagcca
    51601 gtttctcggt attaagccaa atttatacaa aaatgtggaa agccatttca caaaattttt
    51661 cttaatacgc gaaactaagc gactttttca caacctctcg ataaacggtg ttcaatcagc
    51721 tgaccctcgg tattagctca aaaaatggca aaatatgagg aactattttt ccatttgtct
    51781 ctctaatact caggtcagaa cgctgatttt acaacctctt aataaacggt gttcaaaagc
    51841 ttgcatctgc ggtaataagc ctaaccaaac gaaaaatagg agaagctatt tttgtttggc
    51901 tgttcttatt acttgatgca gggcagcttt ttcacaaccc cttattcacg gcgttccaga
    51961 agtagcttct tcttatttct tgaagctgat aacttctgga acatcctctt tttttatttg
    52021 gacggataat ggtcattcca tttctttgat catttttttc attttgtcga tctcttcttc
    52081 ttgtgcctga atgatttctt ccgctaattt ttttacttca ggatctttta aatcagctcg
    52141 tttacttgcc aggatagcga tagaatgatg aggaatcatg gccttcatcc aagctgtgtc
    52201 gttgacagta gtttggcttc gtaccataaa caaggaaccg gcaaaaacaa gaagactagc
    52261 ggctaagatc ataacattta tctttttgtt ttcatacatt ttccacatga acaacagcat
    52321 gatgactgcc atgactgcac ccatcatcaa cgccatgaat actcgtgtac gactgaagaa
    52381 gatatgatcg agcgcataga cattcaagta catcatacca aacataacta ccgttgaaac
    52441 accgatcatc aacaaaaact tccaatactt tttcatttaa aacaaactcc tttttttcat
    52501 tcctttcttc tatcatgtca tgagcatgga gaacaatcaa ttattttgca cagagggttt
    52561 ttgaacaccg tttaccaaga ggtcatgaga ctgtttattc ggaaaaagga gttgcaacaa
    52621 gacttttgtc acaactcctt tcttcaatag ctccatctat tccacataaa taaaataaaa
    52681 ttttctatcg atttcattca ttcgtcaata cgaacgcatc attctttttt tgataactga
    52741 aataaatgaa caggtaaagc aggccagtca atacagttac cccaatggag ttcgtgaaca
    52801 ggataccaag gaaaatcaat cctaagacag ctaatgcaaa aaagctgatt cctttatccc
    52861 ataatgttcc ttttcttagt gccagtacaa aggatgccgc actgataatg atccagatca
    52921 ataatactgt atacgaaagg gaacctgcca gatagccaaa taatttgtca ccgacaaaat
    52981 aagaaagagc tacaccaaga taaagaacac tggtacaaaa taaaacagca cgttgcggta
    53041 cttgatgctt gtttaaaaca gctagttttc tagaggaacc ttttcgatcc ttcaaacgga
    53101 agaacaaaat tcttgaagat gcatacacac ccgaattgat agatgagaac aaagcaagga
    53161 caatcacaaa attgacgata tccgctgcaa atgggatatt cattttttca aataccatca
    53221 caaaaggact gacattcgtg cctgctaaag tattccaagg aaaaagaatc aaaagcaaaa
    53281 acaaaggaat gatgtaaaag gaaatgattc ttcccatgac acctttgatt gctttaggaa
    53341 tcgcacgttt aggatcatct gcttcgctta ccgtgatagc aatcagttca gaaccgccgt
    53401 aagaatagat aactacaagc aaagaactga tgattccctt cataccatga ggagtaaacc
    53461 caccatggtc agtcaattgc tgaagggtag ggaaaacgcc tgtgcccaac atttgctgac
    53521 cgacaagata agtagcaaaa atgatcagta agataatgac actgatttta gcaaaagcca
    53581 gccaatattc tgtttctgcg aataaccgta ccgaataaag attgatgatc gtagttaaaa
    53641 cagccaacat aaaaacaaat acccaggctg gaatgtgcgg gaaccacaac tgcaaaaagg
    53701 aagcagctgc aacagcttca gcgatgatat tgataatcca catagaccag tagacccaat
    53761 cagtaaaatc agcagaatga gcacctagat aagggcgtat caaaccagat aatccgtgat
    53821 gcgtgtcgcc accgcttaaa acgatttttc ccagaccttt catcacgata tataaaatca
    53881 gcccaccaat caaaaaagca atcaatacag aagggccggc gatcccaatc gcttcgccac
    53941 ttcctttaaa tagacctgca ccgattgctc cacctaaagc taacatggtg atatgtctgg
    54001 agctaagttg ttttttcaat tttttatctt ccataaatga cctctttctt ttaaattcac
    54061 aatcaaaatt tatattaaga ataaatagca atatttaaca atgactaatg ataacaaatt
    54121 ttcagacaat tacaactatt cagaaaactt ttaggcgaat tttaatagaa gaatgattta
    54181 tttcatcata ttttcataca aatgttaaat tttcatacaa atataggaaa cgttttactg
    54241 gctttttact atttaaacag gctatcatag gaaaaagtga cagagaaagt gaggcggaaa
    54301 agatgaacaa aaatgacgta ctgctagttg caaaaaataa atcactttat tttttgcctt
    54361 ccatccgctc tttagatgtt tggtccggaa aagatttact acagtcggaa ttcattaaat
    54421 cagctagaca acagttggca aattatcagc tgatcgtttt tttagattat ggatttgata
    54481 tggagatggc tcgacgcgtg cgcccattta ccaatgcaaa aattgtgtta tttttttgga
    54541 atcacttcaa agcagaacac gaggcgatgc tgaagcaggc acaagatgaa ccggcaatcg
    54601 atgagattta tcattttgat tttttggaag caaaagaact tgggctgaaa cataatagtt
    54661 cattttattc gaaacatatg aatttttctt ctagcgaacc aaagacggat ctattttttg
    54721 gtgcaacgaa taatgggaga aaagaacgag cagaaagcta taagcaggaa tttataaaaa
    54781 gagatgttac gaccaactat ttcattcttc cacatcgagg gaatgaacaa cctggatatc
    54841 ttacttatga agagtacctg aaaaaaacag gggaatcgcg cgggattctg gaactgttgc
    54901 gggagggtca gcaaggagtg actttgcgga catttgaatc catgtatttt caaaagaaac
    54961 tagtgacgga taatcaagcg attgctcatt atcattttta tcgtcctgaa aatatttttt
    55021 tgcttcgaga acgtgattta gatgaattac cggaatttct gcatacacca tttaagccaa
    55081 tcctatcaga gctattggaa ttttatgatg cagaaaattg ggcaaaacga tttatcacta
    55141 acgattccac tatttatgag caatacgaat atgacctctc ctctgttgaa aacaaataaa
    55201 aaacctacat tttgaagaga ttatgtactg aaacaaacaa gcacaattct tatggcatca
    55261 tgagagttgt gttttctttt tgatctttta ttgggcttaa aaaaagagaa ccagctgctg
    55321 attcagctga ttctcatcag agagcctttt attctgcgcc aaggattcct tcttttgcat
    55381 ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat atcatatttt tgtccttttc
    55441 gccaaatggc accattgatg tagtcgattt cagtcaaacg atggtttttg atcaggtctt
    55501 ggtacataga tgggtaatgc aacccgatgt tgtcaggatc ataagtagaa gcaatatgtt
    55561 ggtacacttc ttcttgatct agtatgattc cttcttttgc tgcgatagaa gcaaattcag
    55621 atacgattgt tcgaaccatg ctctctgccg ctttttgcgc gcctagttca gcaatattac
    55681 aatccaaaat cgtgcataaa ccattcaatg ttccatttac acaagctttt cgccaaatgg
    55741 aataacgaac gttatggcta tagatcggat ttaaatttgc ttcttgaaag acttctacaa
    55801 ctttttttgt aaatgcttcg ccttctggtt ccaagttctc tagttcgatt tctccatcac
    55861 ctaacaatgt gacttttccg ggaccagcta gaccagctgt ccacatcgtg atcccaaata
    55921 agatattttt ctttggcaca tatttttcca gtacatcttc atgacctaat ccatttagta
    55981 aacacaaaac ataagtattg tcagtaataa tggattggat cgaacaaaac atatcatcta
    56041 attgattggc ttttgttaat gctacgatca agtccacgtg ttcgttgctt tcgatgattt
    56101 cttctggtga atagattggc agttttgcga ccacttcttt accgttaaag tcagcaatca
    56161 acccatttgt acgaatggct tcaatgtgag caggccaacg atcgatcaag aggacctcat
    56221 ttccgctttg atgtagcatc aatccgattc ggctgcccat tgctccggcc ccagctattg
    56281 cgattttcat gttttttctc tcctttattc aatgagttcc cttttagtat atagaaaaat
    56341 ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa aagtgtctta cttaagaaga
    56401 aagtgaggga tacagatgga tattttggcg gcaattgatt catttaaagg aagtgcttct
    56461 tcatttgaac taaatcaggc agttctaaat ggctgggaag atcgtaaagg aagaaaaatc
    56521 aatgtgccga ttgcagatgg cggagaaaat actgcagaag caatctatta cgcgctcagg
    56581 ggaacatggc gtaccattga tgggatggat ctcttattcc gtcagcgaaa aatcagctat
    56641 ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg aatctgcaca agcattgggg
    56701 ttagatctac ttgataaacc aacggatcaa actatccgac ttgcttccag ttatgcctta
    56761 ggagaactta ttaaagatgc actgcttcaa gaagtagaac aaattattgt cactttagga
    56821 ggaagcgggt gtagcgatgg tggtctagga ttactccaat ccttgggtgc ttctttagaa
    56881 gggtacacag aaggaaatcc tttattatct actaaaaaac ttcattttga tcgaagaaac
    56941 gttcttgttt ctaaagtaaa attgattatc gctgcagatg ttaccaatcc atatacaggt
    57001 caacaaggag ctgcttggca atttggaaga caaaaaggag gaatagatac gacgctagct
    57061 ttcttagata aacaagcagg aaagattgct cagcaaataa aagatttctg ggaggtggat
    57121 ttagatgata ttccaggaag tggagcagca ggcggtattg gtggagccct ttttttgttg
    57181 ggagcgaaga tggtttctgg tttcgattta gtcagccaaa tcactggatt ggaaaaaaag
    57241 atcgaacaag cagatgtcat cattacagga gaagggcgga tcgatcagca aacgatccac
    57301 ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat atcgaaaacc aataatcgct
    57361 atttgcggaa gtagagacca agagctagaa ggactttctg attttctccc aattgtctta
    57421 agtattcatt tgggtccaat aggtttagaa gaagcaatgg aacacaggca aacattagaa
    57481 aaaacaaaaa tcttaggaca gacacttagc agatttttca ccatcctccc gaagtagttg
    57541 acaaaatcat tttctttatg ctatcgtaat ttcaaatata ggacaaagtg agggatccgt
    57601 gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt gtggagacaa
    57661 ggaagcagtg tgcagatttt cgggattggc ttggtttttt cgatgtatgg gtctttttgt
    57721 gtgtatccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta
    57781 cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc tacgacagcc
    57841 aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg aaactaggac
    57901 ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa aacaaactac
    57961 cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa cagacaaaag
    58021 ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag atatgggaaa
    58081 tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga gaattcagca
    58141 cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg gatgacactc
    58201 atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga aaacaagtag
    58261 cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac cggggattta
    58321 tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag
    58381 ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa
    58441 atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa aaagccgaat
    58501 ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc gatactgaat
    58561 ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg aaacgatcca
    58621 aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta ttaaaagata
    58681 tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag cgacttttaa
    58741 gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt cattttacaa
    58801 tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag tcatccatta
    58861 tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatac cagccaaaac
    58921 atctgagcat tagttatgca agccaaaatt atgaagacaa tcgaggaacg ttggcggaat
    58981 ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga aagcttggga
    59041 tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa cggaaaaaag
    59101 tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat gaaccattga
    59161 attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac gtgaaacctg
    59221 ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact gagattattt
    59281 ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt aaggggaact
    59341 ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa acaacttgga
    59401 acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa ggacagatgc
    59461 aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg aaaatcatcc
    59521 attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt ctcagcttgc
    59581 cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat ggactgccaa
    59641 gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc ccaaatgggg
    59701 agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc atcgaagacg
    59761 aagaaaatgt ctatctgtta gtttgccata atgccttgat aagagtgatc aatgcttatt
    59821 ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca gaattggttt
    59881 cttatgagta attatgtttt tgtgttattt aaataaaata tttcaaggca ctgcttttgc
    59941 ttagcagtgc cttttttagg ttatgacaat tcttttgata atataaactc aacctttaga
    60001 aatgaaattt aattatttta ataaaagtat tgaaattttg tttctttaga tatatactct
    60061 agtcatagaa atgaagacgg atacagaaaa agagggataa tatgtttggc ttttcaatat
    60121 ttatgaacga ttctttgtca gaagagaaga aacagtatat ccgccaaatg gcatccaatg
    60181 gctttgtcgg tattttcact tccatgcata taccagaaga tgatgtttcg gcatatcaaa
    60241 agcgattgat cgaactagga agcgaagcga aaaagaataa actagaattg atggtcgata
    60301 tttcaggaga ggcattggaa cgagcgggtt tttcattaga agatctaacg cctttgaaag
    60361 caattggtgt aacaggttta agaatggatt atcatatttc caatcagcag attgccgact
    60421 ggtcacatca attgaaaatc agtttgaatg ctagcacgat cacgcctaaa gatatcgatg
    60481 aattgaaaga agcagaagct gatttctcac agattgaagc ttggcataat tattatccac
    60541 gtcccgaaac agggctggac aaagaatggt atcagaaaaa aaatcaatgg ctcaaaattc
    60601 aaggtctcct tgtccaaggt tttgtaccag gagatacaga gttgagaggc ccactatatc
    60661 aaggattgcc aactttggaa gaacatcgag ggatccatcc tcttgcaggc gctctagatc
    60721 tattagcaag taacacggat attgtttaca tcggagatgc aggtctgtca gaaaacgttc
    60781 aggagcaatt tgcttctttc caaaaagaac agacagtctt gctccatacg gaacctgtag
    60841 atgaagaatt ttacgaatat atattaggga agcatacgaa tcggcaagac gatgcaagag
    60901 atgtcatacg aagcgtagat gcacgttttc gagagattcc gcctatccca gcaagaaata
    60961 cagctacgcg tatgaaaggg tctattactt tagataacga aaagtatttg cgctatatgg
    61021 gtgagatcca attaaccaaa tatgatttgc cagcggatga aaaagtgaat gtagtagcta
    61081 aagttatcaa agaagagcta ccattgatca accaaatcaa agctggtatg aactatcaat
    61141 ttataagaaa agaaggaaga taaaaatgag caaaatcaat ttagagaacc taacaacgga
    61201 acgcagaaac caagaaactt ttggattaga tgagatgagt atttctcaag ccgttcaact
    61261 aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa aaagaactga caagtatcga
    61321 accagtgatt gctaacacga ttgagtcctt taaaaaaggt ggacgtctga tttatatggg
    61381 agccggaaca agtggaagac taggtgtgtt agatgctgct gaatgcgtac cgacattcgg
    61441 tgtagaaccg gaaatggtca taggtctaat tgctggtgga gaagaagcaa tgactgtagc
    61501 tgtcgaaggg gccgaagatt cttatgagct agggcaagaa gatctaatca agctgaattt
    61561 gacaaaaaat gatatagtta ttggtatcgc agctagcggt cgtacaccat atgtgatcgg
    61621 tggattagat tacgcaaaat cgatcggtgc tgtcacagga acgatttctt gcaataaaca
    61681 agccgaaatc agtaaacacg cggatttccc aatcgaagtc gatgcaggtc cagaattttt
    61741 gacaggctct actcgattga agtcaggaac agcacaaaaa cttatcttga atatgatttc
    61801 tactatcagt atgattggta taggtaaagt attcaataac ttgatggtcg atgtcaaacc
    61861 aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc atgcaagcga cagaagctga
    61921 ttacgaaaca gctgaaaagt actttgaaga agcagatcaa aatgttaagc tggctatcgt
    61981 catgattttg acaaatagtg ataaagagga agctcaagca aaattagctg aagcaaacgg
    62041 atttgtaaaa gggaccttat gagggggaaa agaccatggc aaaaaacgaa ctgactttgg
    62101 aagaactggc aaaacaaatt tataaagggg caggcggcat gggcaatgta gaaagcattg
    62161 tccactgcat gactcgtgtg cgtatgaaga tcatcaatga tgatcttgta aatgtctctg
    62221 aattaaaagc gattccagga gtgcttggtg tagtagaaga tgaacagctt caagtcatca
    62281 tcggacctgg gaaagtgaac aaagtagcac agcaaatggt tgatttggca ggcgtgaaat
    62341 taggagaaaa actttcttct gtaagtacag ctacagcaaa ttcatcagca tctggaaaag
    62401 accgtgtaaa cgaacgggca caagaaatga aagcggcaca aaaatcgaaa caaaaaccat
    62461 ctaaaatcaa atcggtatta aaagacattt ctaatatctt tgtgccaatg attcctgcat
    62521 ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt agcaaactta gtcacagccg
    62581 gaacacttga tgcagcaaca tggcaacaat atatcgatgt gatgaacatc ttgaaaaacg
    62641 ggatgtttag ctacctagtt atctatacag gtatcaatgc tgcacaagtt tttggtgcaa
    62701 caccaactct tggtggagtt atcggggccg ttgtgatgct gacagggatg aatccggaag
    62761 caccactgaa aaacttattt actggtgaag ctcttgcagc tggacaaggt gggatcctag
    62821 gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa aaaactgcat aaagtagtac
    62881 ctgatgcggt agatatcatc gttacaccaa ctctttcatt gattgccatc ggtctgattg
    62941 aaattttctt catcatgcct ttagcaggtt tcatttctga tggcatggtc ggtgggatca
    63001 actgggtact tggcgttggt ggagcatttg ccggcttcgt tctaggcaca ttattcttgc
    63061 cgatggttat gttcggactg catcaaattt tgactccaat ccatgtgcaa atgattgatg
    63121 aaactggtcg tacgctattg cttccaattc ttgcgatggc tggtgcagga caagtgggtg
    63181 cagcattggc attatggatt cgctgcaaaa aagataaaga attaacagaa atgatcaaag
    63241 gagctttacc agtcgggatc ctaggaatcg gcgaaccatt gatttacgga gtaacattac
    63301 ctctaggacg cccatttatt actgcttgta tcggtggagg tatcggcggg gcagtgatcg
    63361 gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc aggagccgct ttgatcccat
    63421 tgatctctga cggtaaatgg ttaggctaca tccttggatt attggcagct tatgctggtg
    63481 gcttcgttgc tacattcttc ttcggtattc ctaaagaccg tttagaaaaa gaagaattag
    63541 cgactgaaac agccacagta aatgaagtgc ctgaaaaagt agtacctcaa acttcagctg
    63601 aaagtgtgac tatcctctca tcagttgctg acggaacagt tgtttcttta gataaagcaa
    63661 gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta ctttgtagaa ccagaaaacg
    63721 gacatatcta ttctccagtt actggggtag tcagctctgt cttcccaacg aaacacgcta
    63781 ttgggatcac aacgccaaat ggtttagaaa tcttgcttca tatgggaatc aacacagttg
    63841 acttaggcgg tacgccattt gatgtgaaag taacagaagg gcagcaagtg acacctgata
    63901 cgcttgtagc agaagctgac ttagaagcga tcaaagcagc aggcaaagaa acatccatga
    63961 tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt cttggaaaaa acaggtaaag
    64021 caaaagcaaa agcacctgtg atggatgtca aagcttaata tgatagaaac aatagaaacg
    64081 aaagaagaaa ctgaaatcta tttttcagct tcttcttttt tattatttat tgttttctgc
    64141 taaaatctga taaattcgtg tcacatcaga tggtgttccc cgtaattcat aatctgccaa
    64201 gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt agacaatact gattattgaa
    64261 gtttttattt ccgctaccga ctacaccaag acaaagttta tgattgtctt catgttccag
    64321 atattcacgc atcgtttcgg tcaagatttc tgtatcaccg ttatctaaac catttccgcc
    64381 gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt tctttcgtaa aatcactatt
    64441 ttcatggatt tcttttaaag taacttcagg attgacagga tcttctgcat gcattttttc
    64501 agcatattct gctagatgtt tggcaaaggc acgcgtattt cctgaaatcg atatatataa
    64561 aatgttcatg gatcatcctc caattctttc cttttagtat agcatctttt ttaagttttt
    64621 tggtggtgta atttggaaag ggactaggcg ctttgcttgt ataatgagaa aaaagccggt
    64681 agaattatat ttctattaaa gtttttatta attaagtaaa aataaaaaag gtttgttata
    64741 ctttttgata aaagcggttt tattttggag gatgcttaca tggatacaga gcaacagaaa
    64801 aaaggaattg gtttaattcc acttgctgcc ttagtaattg gttcagcgat tggaggtggc
    64861 gtatttggta ttatgacgga catctccgga tcggctggcg gcccagctat tttcagttgg
    64921 gtactcgtag gaacagcaat gttgatgctc tccttgtcga tcaataatat caataaaaaa
    64981 cgtccagatt taaaaggcgg gatctttagt tacgcagaag aaggttttgg ccgtttcgct
    65041 ggttttatca gcggttgggg ttactggctc acatgctggt taggcaacgt agcttttgca
    65101 acattgttga tgagcgcatt aggatatttc ttcccagtat ttgaaggtgg gcaaaatctg
    65161 gcatcgatcg tgatgagtac agtcttttta tggggatatg catggctagt gaatcgggga
    65221 gtcgaaaatg ctagtttcgt caatgctatc gtcacattct gtaaattagt gccattattt
    65281 ttatttattg tagtagctat cagcacgttt tcgatccaac aattcattga taacttttct
    65341 agcgacatta tattgaatgc gaatggacag ccgatcccgt tttccagtca agtattgagt
    65401 tgcatcatgg tgatgctttg ggtatttgtt ggtattgaag gagcctctgt cgtaggttca
    65461 cgagcaaaaa aacaatcaga tgttgggaaa gcaacgattc tagggatatt cggcttgatt
    65521 attatttacg tcttggtttc catgcttcca tatgggacaa tgacgatgaa tcagctgcaa
    65581 acgatcaagg cgcagcctgc tatgggctat gtgtttgaaa tgatggttgg taaatgggga
    65641 gcagtcgtga tcaacggcgg tttgatcatt tctttagttg gtgtttggct ctcatggacg
    65701 atcttaccga ttgaaacaat gggaaacatg gctgaagacg gacttttgcc taaagcatgg
    65761 ggaaaactca acaaaaatgg ggcaccaact tatgctatcg tattgacgac gttatgtacg
    65821 aatgtctttt tactttcatt attattcact gacagtgctt ataactttgc ttacacactt
    65881 ggtacagcag ctattttctt cacgtggctt ttcttaggat tgtaccaaat gaaactttct
    65941 tatcagcgga aagaatgggt ccaattttta gtagggcttt tagcaagtgc ctttcaagta
    66001 tgggctatgc tagtcgtagc ctttaatgaa gtaatgcttg ttcttgttct tttcttgcca
    66061 ggcatttatt tttacctcaa agcacgtaag gagcaaaacg agcctgtaca acatatccac
    66121 ttagataaga ttttacttat aggtgtgacg atggtcggag tgattgcaat ggttttattt
    66181 gccactggga tgcttcacgt atagaagtat aaaagaagta taaagagagg ttgtgagcct
    66241 gtcgtttatt caaaaacata ctaggaaagg agctgtgaca caaatgttgt cacagctcct
    66301 ttttcagaat aaacggtgtt taagagacag ccaatcggca ataaggctga acgtctcttt
    66361 cacagcctct tttttgatga gtcatcatac attttttcgc ttaaatacga gatatcctaa
    66421 tgctaagaac aaaacgatgt aagctaaatt accgaatagc atttgattag tagaaagatg
    66481 agtcaattgc ttcatgatct cttcgtttcc aatttggttt tgcagattca acatactgat
    66541 cggattccat ttgatccact cccatttttg gatgagtgca ataaggatac cagatatgat
    66601 tgacgaagca aaataaaaga cgattcctgc tgagatggat gcgccggaac tattgataaa
    66661 gcaagcaatc atcaacacta aacttaaaat cagccataaa ccgatataac tacccaaagt
    66721 ataagtgaac aagctttgga tcagtgtttg accagtagaa accttctccg tgaaggaaat
    66781 cgaagggaaa agaatcagtt tcatcaacac agtgacaata atagctaaaa ggtataaata
    66841 aacagaataa ataactaatg tgatccattt gcttactaat atttctcctc gagaatactt
    66901 tcgataaagg agatttttca atgtaccata ttgtgcttcc atcgaaatga tcgtgcttgc
    66961 agctgcaatc atgataaaga caatccaaga tgtggcacta aaaagctgag cagtcatttc
    67021 aactggatca atgatcgtcg tgtatttttt ggctagaagt gctgttccta tgagcaaaac
    67081 aactaataga acagataaaa cagcagttga ctttttcttg atcaatttaa ataattcttg
    67141 ttttacaagc aatgtcattt ttcttcttcc ccctttactg aatccagaat ttccaataga
    67201 gaagattcca aatcattcgt cagatgctgg acgtctgtca catggatatt ttgtgcagac
    67261 aatagctgga tcacggttcc taataattct tcgttttctt ttaaaagctt gattttatca
    67321 ggatcttcca aagtatatcc agcttctgtc aagatcgttc gagcagctag atcatttgtt
    67381 gtagagataa cgatgaaatc ttgcgctgtt tggttcaatt cagccattgt acattgtttg
    67441 atgatctttc cttgattgat caggatcagc tcatcgacca ttttttcaag ctcgctgagt
    67501 aagtgactgg agatcaagaa agaggtgccc tgagtagcca agtcggtaat gatttttcgc
    67561 acatctcttg ttgcttgtgg atccaaaccg ttcatcggct catctaaaat aacgatagac
    67621 ggatggttca gtaaggcaag agcaattccc aatttttgtt tcattcccaa cgagtaggat
    67681 ttcgcttttt tgtgaatgta cttttccatc ttgagctgat tcacgatagt gtcgatagca
    67741 gatacatcgt tggtttcaga gaataacttc agatgatcat agcctgttaa gaatggatag
    67801 atacccgggt attcgatcaa tgctcctact tgttccaacc ctttatgact agagggagaa
    67861 atctcatttt caccaataca gattgttcca tctgagtaat ggatcagtcc taaaatagct
    67921 ttcatgatcg ttgtttttcc agcgccatta ggacctacta atccaacgat tttgccaggg
    67981 gataagctaa aactgacatc gctgagggct tgtttgtgac caaagttctt ggataattct
    68041 tggaccgaaa gacttttcaa aaatattcac tccttttcta tagatagtac aagcatacac
    68101 ctgatgagtg gagaagtcgc taggtcattg gacttatttc ttattttttc ataaaaaaat
    68161 tgaatattaa ataaaaaggc ttattttttc tgatcagtac ttattccaag tgtctagtgg
    68221 ccgctagtaa tcttgatgcc aatgatactg atgagtagca tccccacaaa gaaccaagtg
    68281 gaaagattca gcttatcatg aaacaagacc acaccaatta tgaccgaacc aactgcgcca
    68341 atccctgtcc agataggata agagagactc atcggcaaac ttttgattgc ctttgacaaa
    68401 aagtaaaaac tagcaatcat gccaatcact gtgtaaaaag aataattgat tttagaaaaa
    68461 ccatcactcc atttcatcat cgttgaccag aatacttcca aaattcctgc tatgattaat
    68521 tccatccatg ccatacttac attcctcctt aactataaaa aaatacacaa aaagaggaaa
    68581 atcatccctt caaagaccta atggaatgat catcctcttt atcctacccg gtggcagatt
    68641 gtatatcttt ttctgtagta tagcacacct atccagatat cggcaatctc atacttttta
    68701 gtaaagtgta ccaagcactg acggatatat cgttagcgga aaagaagatg aatgctttgc
    68761 aatttcatag aaagaagttg ttatttatta atcatttaaa aaaaacagta actagacctt
    68821 atttctatat attatcgggc aataaattaa aaaaagcttt accttacaat aagtaagata
    68881 atttttgtga taaaaaaata acgaaaaata tttaaaaata gtgtctattt cgtgagaaaa
    68941 caactatatt ttttatgtgt tttgctttat aattaaaaaa atattgtcat tttatggagg
    69001 aaaatatatg gaaaattatc ttagcctttt aggcgtcttg attgttattc tcgggtttgc
    69061 atttaaatta gactcgatat tgatcgtaat ggttgcttta gttgttacag ctttgactgg
    69121 cggtcttggc attcaaggaa tgttagaact tttaggtaca agttttgtaa acaatcgcgg
    69181 gatggcgatt tttatcatca ttatgttggc aacaggaacg cttgaaagaa atggtctgaa
    69241 agaatcagcg gcttccttga tcaagcgatt caagaaagta tctgctggta tgattgtaga
    69301 tatttatggg atctttcgaa tgatctttgc ggcttttaac gtgagttttg gtggagttgc
    69361 tggatttgtt cgtccaatca ttttaccgat ggcattgggg accatcgaat caaaagggtt
    69421 agaagtccat cctaagcatg aagaagaact aaaaggtatg gcttcagcta tggaaaatgt
    69481 ctgctggttc tttggccaag tactattcat cggaggagca ggggcgttgc tagtccaatc
    69541 aacactgaaa gatctaggat atgacgtaac attgggacag cttgctttag tggaagttcc
    69601 agtggctatc acggctttag ttgtggcaag tgtctacttc tatcttaaag aaaagaaatt
    69661 agcgaagaaa tattatccag gaaaggggca ataagatgga cttttttaca aatcctgata
    69721 ttttattagg cgataaatta ctggagatcc tttatatcgt gatgggactc gtattgattt
    69781 acactggagt aagaaattta acagataaaa ccaatcctca tcgttatggg tcagcctttt
    69841 tctggacggt cttaggggta gttattgcag gaggaagatg gcttccggca atcgtgaacg
    69901 gtggcttgat ctttgcgatg accataccag caattgctaa gaaagtcagc aaaggagaaa
    69961 gccggcttcc atcaaaagaa tatatggaaa aaatgtcaga caaactagga atgaaaattt
    70021 ttattccagc tttgagtatc ggtgtctttg cgatcttgtt tgctctattt accaatttag
    70081 gtgctttagt cggcgtgggc gtcggtgttt ttgcagccat gctaatcttg atgttctttt
    70141 ctcgagataa tcgaccaagt acatttttag atgatgcagc agatatgctg ggcacagtag
    70201 ggccgttaag catgttgcca atgcttttag cttctctagg tgcggtattt acaagcgctg
    70261 gagttgggac agtgatttcc aatgcagtag gtacgatcgt tccagaagga aatgtgaaca
    70321 tcggcatcat catttacgct cttggcatgg tgatatttac ggtaatcatg gggaatgcat
    70381 ttgcggccat caccgtgatg acggttggga tcggtgcacc attcgtattt agtcatggag
    70441 cgaatccagc gttaatagga atggttgcat tgacttgcgg tttttgtggg accctgctga
    70501 caccgatggc tgctaacttc aatatcgtgc ctgtagcgat gctggagatg aaagacaaat
    70561 acggagtgat caaaaaccag ttgtttattg cgttatttat gctagttttc caaattgctt
    70621 atatgattct atttaaataa tagaagagag gggttgtgac agaataattg tcgcaacctc
    70681 ttctaacatt gtagtggaac actatttttt tataagaata tattaagttt gatctgttat
    70741 gttatttaaa ctattgtttc ctttatttac gctgttcaag gaaaatggta tgataaaaga
    70801 taaccatatg ttaaatcaat cggacggagg aacaacagat gaaagtacta gtaacaggtt
    70861 ttgatccatt tggtggggac aaagtgaatc cagcctatga agcagtaaaa aaaatgccag
    70921 atgaaatcag cggtgctgaa atcatcaaag tggaagtacc gaccgtattc gaaaaaagca
    70981 gtcaagtagt caaagaagcc atccaacagc accaaccaga tgtcgtgatc tgtgtcggac
    71041 aagcaggtgg gcggtcagca gtctcgttcg aacgtgtcgc aatcaatcta gcagaagcaa
    71101 gaatccctga taatgaagga aatcagccat tcgatacggc tttagaagaa aacggtccag
    71161 ccgcttactt tacctcattg ccaatcaaag caatgacaaa aaatgtacaa gatcatggtc
    71221 taccagcgta catctcttat acagcaggaa catttgtctg caatgatatc atgtatcgtt
    71281 tgcttcattt gatcgaaaca caattcccgg caattcgagg tgggttcatc cacgtgccat
    71341 tctcacccga tcaagtgatc gatcgtccag tgggtacgcc ttctatgtct ttagaagaca
    71401 ttgcttcttc tttgacttat gcagttgaag cagcagtaga aaataaagaa gatatttccg
    71461 gaaatgccgg aacaactcac taatttagca agaaacactg gctggttgct aacgttatgt
    71521 tagtagccag ctttttgtaa ttagaaagga aagaaacagc cgtggaagaa tggcaatccg
    71581 tctttgaaga atggtttcca aaagaaatca gcaaaagcta tcccatcaaa atttctaaac
    71641 aatacacaag cagtcaacgt tgggagatct atgcaaaatt aacgaaaaaa cagcgggaac
    71701 tagtcgataa gcaccgccga tatctaatca gctctcgttt tatggaagaa cattatttag
    71761 ctgcaacaga ttgggtgttt tctgacttca agatcaatcc gttttttcga acaaaacgaa
    71821 gccagcaaaa attgtattgt gagtgtggta gagaattaaa agtgcaatac atcgttaaat
    71881 cgccaaaaac cggaaagatc ctaaaactag gcatcaacca ctttgccgat cacctgcatg
    71941 tctctccgac agtggcagct tcgatccatc agggaatgac aaaagttgat ctggccttag
    72001 acgaattatt atggcttaag cagaagaata tcgattttcc tgaaggacta tggcaaaaat
    72061 actgttttgt cttgtaccaa aatcgtcgga tgaaacagcc atatttaccg gatataaaat
    72121 tagctcagcg gctcgcagaa tttcgccaag tagaaatgcc gatttacata gcggattatc
    72181 aagcattgga aaatgaaatc aagaaaataa gtgaacacat caatgggcag tcaaaaaagc
    72241 gacagataaa aaaagaacta tttgacgact ttgcagaaga attagtaaag gatgtagaag
    72301 aattcttgat caactatcgc gcatttttac gtaaagattg gcaatccatc gtatatgaag
    72361 aagtgccagt acatcctaat gcttattttg aaacatttat ttccgtgttg agaaagacaa
    72421 aacgtcaacg aacaccagaa gtcactgctc agatggaata ttttgcgaaa aaccaacgat
    72481 tcatccaacc aaaaatctat ctatttattt ggaagcaata ttgtcgctat ggttttacag
    72541 aagggttctt cgacagcatt cctcggatag tcagaaatgg ttttttaaaa gtattgcgta
    72601 aagaacgaga agctatacaa tctgctgata agaaagaccg tacagtctca aaagaaaaat
    72661 ggcaactagt agtgaaagat atccaatctg gaaatgtgca agagacaatc gacaaatgga
    72721 aaggaaaaca ttatcgtttt actgaagctc agaaacaagc gttggaatat tatcagaagt
    72781 tagaagaatc actgcgtttc aatgatgagg caagaaagta tctgaaggaa ttactataaa
    72841 aaatagaggc tgggacagaa gcgtttaact ccaagaaata agaagaaatt cacgaaaatt
    72901 gcttttcaaa tttttgtgaa tttcagctta tttccgaagg agttgcttct gattccgccg
    72961 tttatacgtg tttagagcgt gagacaaaag tgttctttac ttttgtccca cgctcagttt
    73021 ttttatgcgt gttgttgtgc gttgtagtat tcttcttcca ttttttgcat acgttcacgt
    73081 tctttttggt tataccaagg tgaaacggta aatgcaagga catcattcag caagtaaaca
    73141 gagctgttca cgaacatcgc caaacttgca gatccatgcg taaatgaaat ataccaaagg
    73201 accatttgag caagccctga agctaaccac cagtaatatt ggttgttgta acgcaagaaa
    73261 ctgatgatac cagcagtcaa gctaatagaa aaagcaatgg catctaccca cggccgagga
    73321 tcatctgtat aacgaccaat caggaatcct gaaacaagat agacaaggaa agtactgatg
    73381 attgcgataa accagctttt tccagtgaat tttcggattt ttgaagccat attatgattc
    73441 cattctttac ttaacaatac ggggatatcc aatgtgatca tataagcaat ctgttcaccg
    73501 atacttaaat agttctttgc tgaaaaccca acgataataa aacaaatcgc agacaaaata
    73561 cctaaaatac cgttgactga ttttgccgca ttgatggata aaatacacaa tacaccaagc
    73621 gtggtaccga taaatgtaat aacggacagc caagtgatgg gactattgac aagagtcatg
    73681 acctggcagc caagactgaa aaagaaaaga taataatttt gttgcggcca gccttttagc
    73741 tgatgagcta aaaactgaaa atatgacttc atgtatgagg actccttaag acactaaatg
    73801 tagtgtgttc tgtatttact aacacaatct attgtatgct tttcttgaaa aaaaactagt
    73861 ctttcaatta taaataattt gtgttatgat aattttctgt ttgttttctt gaaaatataa
    73921 tttcttactt tcatttatta tattgggaac aggaagagtg tcttggagga cctactttct
    73981 gttttttttg ccttttttct tataaaaagg aaaaacagct atttttttca atctgacaca
    74041 tttacaatgg aactggagat taatatagat ccgagcttaa acgaagaata ggagtgctca
    74101 tatgaacctt acaacgatcc atcatatagc catcattgtt tcagattatc agaaatcacg
    74161 tgatttttat gttaataaat taggatttga agtaatcaga gaaaattacc gcaaagaacg
    74221 agatgactat aaattagatc tgaaactagg aagtgctgaa ttggagattt ttggaattgc
    74281 taatgcgcca caacgaccaa actatccaga agcttgtggc ttgcgtcatc ttgctttcaa
    74341 agtagacgat attgaagaaa caattgcaga attgcagtcc ttagggatcg aatcagaacc
    74401 gattagagaa gataccttta caggaaaaaa aatgacgttt ttctttgatc cagacggatt
    74461 gccattagaa ctgcacgaat aaagataaca aaattgtcac ttttttttcg tttcattcga
    74521 tttttcattc gttcatttct tgatattctt taaaaaaaag aaacaggaga atgaagaatg
    74581 aaaaaaatga tgtttggtgt tgctgccctc atcgtaatag ggattaccgg tatgacggga
    74641 ctgctcaatg ccgcttttca catggatgat attagtgacc aggaatatcg gatctcattg
    74701 aaaaaagcag cagcaatcgt aaaaaacgaa acaaaagaac ataacatatc tgctattcat
    74761 tttgcagttg aaaaagattt ggagaatcct acgaatgcaa catatgaata ccagtttttt
    74821 tgtgaaggac aagtcatagc agtcaatcca tccactggga aaacaaccgt acgaaatgac
    74881 acggaaaagg ccgcagaaca tctttttgat atagaaaaaa cggaagagtt caaacaaccg
    74941 caagcagcga tgaaggaagc agtcagctac tacgggaaaa aagcaaaagt gaaagagtgg
    75001 acacttctag tcaaagatga acgtccttat tataaagtcc gattaataga tggagaacag
    75061 gaccgtcagg tatggatatc tgcatagaag aaggatattg cttctctttt ttacaatata
    75121 agagtaagct aagagagtaa agatgaaagg aagttgagat aacatggaaa aaatgaaatg
    75181 ccctaattgc ggaaaaaaat ttgcatacga agaagtcaat aatgtagtcg aacataatga
    75241 taaagaaatg ccagtcgtct gcccttattg ccgaactgaa gcggcaagaa tcgttactca
    75301 tggttatttc attactgaaa agattgaaga ttttttaaaa taacgatttt gcgatttctt
    75361 tttctgcatg ttagaatgga gaaaaggaga ggaaactatg attcgttttg cgagaaaaga
    75421 agatgctcca gaaatcgccc cattgatctt agtgattcta aaagatatgg aactgccatt
    75481 tttgaagcag acaggggaac aaaagacttt agagatttta gaagaagcaa tcactgatcc
    75541 aaattatcgt tacagttatt cacgaggtat cgtagaagaa atagacggta aagttgcagg
    75601 agttgcttac ggttatacag atgaagaaga atcaatcatc gatcagccac tggtacctat
    75661 cttagagaaa tatcagctag atgcgtctac acgtttgttc attgataaag aaacgtttcc
    75721 caacgaatgg tatttggact ctatcagtgt atcagaagat tttagaggac aaggcatcgg
    75781 ttcgcgatta ttagaagcat tacctaaatt ggcgaagaaa gctaatcgct ccgtcatcgg
    75841 tctaagtgtg gatgaaaaaa atccaaaggc taaaaaatta tacgaacgcc atgacttcaa
    75901 agttgtcggt cagcggatga tcagtggtca tctttatgat catatgcaaa agaatatcta
    75961 aaaagatgta cctgaaatgc gtatttccgc atttcaggta catcttttta acttaagtta
    76021 agtcggtaag aaacaggctt atcgcgactt ttgtctttac ggtagagcca gtaatctaaa
    76081 accgcagcaa tacaaataca aactggagca tcttcatcat ttgagacatc caatacatag
    76141 taatctcctg aaaaaagtgt tgctttatcc attttcatga tttgctgatt gaaatgatgg
    76201 atttgatatt gatgattata gatatcacct tggactgtcc agtgaagtct acgaatataa
    76261 taaaagtcac cgggccaatt gaatattttc tgcatggttc ccaccttttc gaactcttta
    76321 tacagctcaa aacgcgtacc gaatgtcagg cttatctgct tgatgtgagc aacgagatcg
    76381 ccattcatgg cataaagaga caatgcatcg ccctttgttc cccagcgacc aaccatcaaa
    76441 aacagggaat gtcctttttc gtttttgaca attgtccgag ttgcacgtcc aagctgcttt
    76501 tcctgaatga aaaattctga catctatatc cctcttttct agataaaact acaagtcttc
    76561 tgcaatcgcg cgcaatatgg cttttcctac gccagattca aggtttgtgt ccgttgtata
    76621 tttggcataa tcttttactt cgatttctgc atttcccatt gcgaaactga ctcctgctac
    76681 ttgcagcatg ctgacatcat tgaagttatc gccaatcgtc atgacttctt ctaaagaaat
    76741 cccacgctcg tgagcgacat gggcaacagc aattcctttt tgagcgtttt tatggttcac
    76801 ttcgatattg ttttgaccag aggaagtaac ggctagatcg cctaaaagag aaatctgctt
    76861 tccaactgga cctaatacag ctggcccttc tgtatgaaaa caaataattt ttaaaacttc
    76921 cagattcttt tcttccaaaa tagcacgaat actgtccacg tatgtgatgt gcaataactc
    76981 cagcttagct gaggccatgg caatcgccat tttatacgta aggtgaggta aatgggtagc
    77041 caccatggaa gcaaaacttt caattcgttt cgcttgattt tcagaataaa gaccatcgtt
    77101 ggttgctact tcatagtaaa taccgttagc atctagaata tctaaaacag agattgcttg
    77161 attatttgga atcgacacgg taaacaatga atttccttct ttatcaaata cctgtgcccc
    77221 gttcaaagtg atcatcgcac aatcgattcc tacctcatct aaagctgcgc gagcttcttg
    77281 atagtttcga ccagtagcga ccatgaactc aacaccggca tcatttgcaa aacgtatcgc
    77341 ctcggcattt tcaggagaaa cactcatatg ggcatctaat aatgtgccgt ccatatctga
    77401 tgcaattaat ttgatcataa tgattatcgt tcctttctgc ttcttgttcc tagtttacca
    77461 tattaatcaa gtgctgaggg tgaagattgt cccttgtgat tgaagaaaat gtgagttatt
    77521 ctttttatta ggaggtggag tttttgaaag aaatcattca taacagttgg caagaagttt
    77581 tgtcctcaga attttcaaaa gattattatc tgcatctaag agaattttta aaaaaagaat
    77641 atgcgtccca aaagatccat ccagatatgt atcatatcta tgaagcattg gaattgactc
    77701 cttatgaaga agttaaagtg gtgattttag gacaagatcc ttatcatgga gaaaatcaag
    77761 ctcatggctt atctttttct gtgcaacctg gcgtaaagat cccgccatcg ttaagaaata
    77821 tctataaaga gctttatgat gatcttggaa tcgcacctgt tcaacatgga aatctagtgt
    77881 catgggctaa acagggagtc cttttactca acaccgttct taccgttcgg gaagggcaag
    77941 cgtattctca tcgcgggaaa ggttgggaaa gattaacaga cacgatcatt gaaaaattaa
    78001 atgaacggga aaaaccaatc gtgtttatct tgtggggaaa accggcacaa gaaaaaatca
    78061 aaatgatcga taagagtcgc catatcatca taacatctcc acatcccagt ccgttatctg
    78121 caagtcgtgg cttttttggt tccaaaccct tctcgaaaac gaatgatgcg ctccttgcgt
    78181 taggagaaga accgattgat tggcaactgc ctgaaacagt gtaataaaac agcgctttcc
    78241 tgttatagtg cagttcgctt tgtatgtgaa aaacttaatt tttccagcat ttttgactga
    78301 tacagataaa ataaactgtt ctttttttct ggaatagtgt atgataatac atataaaaac
    78361 gtctggaggt catcttgtgg aattgtttga tagtttaaga tttaaaattg ttcgccgtgg
    78421 gatcaaaatc gttttcccgg aagctactga tgctcgtatt ttaggcgcag ctgcccggtt
    78481 gaaagcagaa gaacttgtgg aacccatttt agtagggaac gaagaagaaa taagaaatgc
    78541 agcgcatgcc agaggaatca aaacatctag ctttacgatc atcagccctg ataattacga
    78601 taagtgggat gaaatggtgg atacttttgt tgaacgtaga aatggaaagg catccaaaga
    78661 acaagcagaa aagattttaa aagatgtgaa ttactttggt acgatgctta cgtatatggg
    78721 gattgcagac ggaatggtct ctggagcgat ccattcgact ggcgacactg ttcgcccagc
    78781 acttcagatc atcaaaacaa agcctggcat cagtcggaca agcggagcga tgatcatggt
    78841 acgtggaaga gatcaagaaa aatatgtctt ttcggattgt gcgatcaacg tgaatccaac
    78901 tgcgcaagaa ttggctgaaa tcgccgtaga ttctgcaaaa acagcagaat tgttcgatat
    78961 cgaaccaaaa gtagcaatga tgagcttctc gacaaaaggc tcagcgaaat cgccagaagt
    79021 agacaaagta gttgaagcaa ctaaaatcgc taaaaaacta gcgccacaat acatgatcga
    79081 cggcgaattg caatttgatg cttcttatgt tccttcagtt gcacaactga aagcgccaga
    79141 ttcagaagta gctggtcaag caacagtctt tgtatttcca gaattgcagt caggaaatat
    79201 cggctataaa attgctcagc gtttcggtaa ctttgaagca atcggaccaa ttctacaagg
    79261 cttgaacaaa ccggtttctg atttgtctcg tggagcaaat gaagaagatg tatataaatt
    79321 atcgattatc acagcagcac aaacattgat tgattaacaa atacacgcga actagccaaa
    79381 aacagcagct gctgttcttg actagttcat tttttcttta aaaaaacttc attacgcgat
    79441 atactggcaa aaagaaagaa gagcgggaga agatgaaaaa tgatcgaact atcaggatta
    79501 acaatgacag aacaattcgc agaggcaatt ggtcattccg caatgccagg tgacaatctg
    79561 atattgactg gtgatctagg tgcgggtaaa acaacgttga cgaaaggaat tgcccaagga
    79621 ttagggataa cgcaaatgat caaaagtccg acatacacga ttattagaga atatagtcaa
    79681 ggtcggattc cgttgtatca tatggatatt tatcgggtag cagctagcgg agcagattta
    79741 gggttagatg aatattttga aggtgatggg ctttccgttg ttgaatgggg aaatctatta
    79801 gaagaagcac tgccggagga ttatttggaa ctgattttag aaaaaagtga tacagattta
    79861 gaaaagcggt atgtgaaact aatctcatac ggggagcaaa gtgaaacctt caaacagcga
    79921 attctagaaa aatgggctga aaatcatgag tgaagaagtt gggatcatct tgcgggaagc
    79981 agtgccagga gatgcaaaag acattctttt gatgatggga caagttaata aggagacaga
    80041 atttttagtt ttggatgaag cagaactcct gttaccacca gaaaccttag aagaagagtt
    80101 ggattatata tacgaaagca ataataattt attgctgctt gcaatctatg aagggacgat
    80161 tattggaaca gcatctgtca aggcagacag tcaatttcgt ttgtctcacg tgggagaagt
    80221 cggtatcagt attctgcagg aatattgggg aatgggacta ggcactctta tgctagaaga
    80281 aatcatcagt tgggcgaagg aaatgggtgt actttttcga ttggagcttg acgttcaagt
    80341 tcgtaatgaa agagccgtac acctttaccg aaaaatgggc ttccaaatcg aagcggtcat
    80401 gcctagaggg gcaagaacag atctaggtga atttttagat gtatataaaa tgagttattt
    80461 gattgaatag aagaaatagg aaaagagtga gaaactgtca tccttttgag aaggcagaaa
    80521 aacacacgaa gggactgtga caaaagtcgt gtcgcagtcc attttttttg aataaacggt
    80581 gttcaatcag ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
    80641 gccgtttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct tggtaaacgg
    80701 tgtccaaaag cttgcatctg cggtaataag tccaaccaaa tgaaaaatat gagaagctat
    80761 ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc tctccaataa
    80821 acggtgttct cctgccagct cctcggtcac cagtcacaat atttaataaa catgagaagc
    80881 tattttttga aattgctcct tgtgactcgg agtgaaacgg caggctcacg acctctttgt
    80941 gtgtttcata agtattcttt tactttatag tcacaagttt tttcaatgga tctgtcccga
    81001 attttttttc ttgataaagc aggatttccg cacatgcacg actatcttcc aatgcatcat
    81061 ggtgatgatc taattggatg ttcaaatttt cgcacacagt gttcaatcga tgatttggaa
    81121 attcaggaaa caggcgacgg ctacttttca ctgtacataa agaaagataa ttaggctgtt
    81181 caagatgata ataatctaag catcccgcta agacctttgt atcaaaagca gcattatgtg
    81241 caacgactaa acgatttttt tgaaaatatg gttggatagt ttgccagact tctgggaatt
    81301 tcggcgcatt gcgtacgtct tcttgatgga ttccgtgaat ttggacattt cgccagaaaa
    81361 aaggagtctc aggctgtatc aatgtataga attcatcaac gatctggcta ttttttacca
    81421 tgactagcgc aagagaacaa gcactataag gctgatgatt ggcagtttca aaatccatgg
    81481 caacaaaatt catttctgta aaacctcctt gggaaattac tgtaacatac ttttgaaaaa
    81541 agcaaataaa tttaacaaaa aacatagaat tttttatgta atatttgttg agaaaagcta
    81601 gtttttgaac tcattccttg tgatttggag ttagatgggg atcatgactt ctcggtatta
    81661 agtcaaattt atgcaaagtt gtgaggaacc attttcaaaa aaatttctta cgactcgaaa
    81721 gtaggcgact ttttcacaac ttctccacta aacggtgttc aatcagctga ccctcggtat
    81781 tagctcaaaa aatagcaaaa tatgaggaac tattttgcca tttgtttctc taatactcag
    81841 gtcaggacgc tgatttcaca acttctcaat aaacggtgtt caaaagcttg catctgcggt
    81901 aataagtcca accaaacgaa aaatatgagg aactattttt gtttggctgt tcttattact
    81961 tgatgcagtg cagctttttc acaacctctt aataaacggt gttcaaaagc ttgcatctgc
    82021 ggtaataagc ccaaccaaac gaaaaatatg aggaactatt tttgtttggc tgttcttatt
    82081 acttgatgca gtgcagcttt ttcacaacct cttaataaac ggtgttcaaa agcttgtatc
    82141 tgcggtaata agcccaacca aacgaaaaat atgaggaact atttttgttt ggctgttctt
    82201 attacttgat gcaggacagc tttttcacaa tctctcgata aacagtgttc aaaagccagt
    82261 ttctcggtat tatgccaaat ttatgcaaaa tgtggaaagc cattttcaca aaatttttct
    82321 taatactcga aactaaacga ctttttcaca acctctttat ttcaaatcta attctactgg
    82381 acaatgatca ctgcccataa tgtccgtatg gatctttgca tcagctagtc ctggctctaa
    82441 atctttgctc gtcaaaaagt aatcgatccg ccaaccagca ttgtttttcc ttgaattgaa
    82501 tcggtagttc caccaagagt agacaccttc ctgggttgga taaaaataac ggaatgtatc
    82561 gataaacccg ttatccaaca gtcgagaaag agccgctctt tcttctggag tgaatccagc
    82621 atttttttga ttcgttttcc agtttttcaa atcaatcttt tgatgagcga catttagatc
    82681 cccacacacg atgactggtt tttgtttttt tagattttcc aaataattat aaaaggcttc
    82741 ttcccattct aatcggtaat ctaaacgttt taattcgctt tgagaatttg gcgtgtaaca
    82801 ggttacaaga aagaaatcgg aatattccaa agtaatcaga cggccttctg tatcatggat
    82861 atcgataccc attccgtaag tggcattcaa tgctggtttt ttagcaaaaa tcgctgtgcc
    82921 ggaatagcct tttcgttccg cgtaattcca atattgataa taacctggaa gatctaaatc
    82981 aatttgtcct gcttggagtt tcgtttcctg cagacagaaa aaatcggcgt ctaactcatg
    83041 aaaaacttct aaaaaatttt tatttacgat ggcacgaagc ccgtttacgt tccaagagat
    83101 aaatttcaat aaaagcacac tccttcgaat taatttcctt acctttgcga tactattgta
    83161 cctcatttta gtaaaaaatg gtgtaagatg aaagtaacta aatgatccag gaggaaagta
    83221 gatgaagatt ggtgtaatcg gtgtagggaa tatcgcagaa aaagcatatt taccaactta
    83281 tgcgaaaaaa caaggaacac ttgattttta ttttgcaaca agaaataaag aaacaaaaga
    83341 aagaattcaa aaagagtacg ggttccaaca tctatatgaa acgatcgatg agcttttaga
    83401 agaaaaaatc gaagcatgta tgatccacgc agcaacaaaa gtacattatg aattagccaa
    83461 aaaatgtcta gaaaatggta tccatgttta tattgacaaa ccactgagtg tccatctaaa
    83521 tgaagttcgt gaattgcaag aattagctga taagaaccaa ataattttga tgatcggttt
    83581 caaccggcgc tttgctccta tggtagaaga attgaaacgt atcccagata agcggttgat
    83641 ccacctgcaa aaaaatcgaa tagcggcaaa agaaacgaca gagttcgtcc tttatgattt
    83701 gtttttacat cttattgata cagctgtgta tttattagat gaacccattt tgagaacaaa
    83761 ataccagata agagaaacaa aggaattttt ggaatatgcc attttacaat tagaaacaca
    83821 aaatcaaacg gctattttaa cgatggattt agcaagtggc gctaatatag aaaaatatca
    83881 agtaacaagt aaaaatggta cttatgaagt aaacgatctg acccagcttg tcattcaaga
    83941 ggctgatgga aaaaaagtga aagaatttgg cgactggaca aacacgctag tcaaacgcgg
    84001 gtttgagcca atggtagagg ccttcttttc tcagatccgt ttaggtaaat cggaaacaga
    84061 attgcttaaa caaaaagaag ttgtaagaag tcatgaatta tgtgaggaag tgcttaaaga
    84121 tcactaccgt catcaacttt gaacaagact ggcaagattg tattaagcgt gatagaataa
    84181 tgaggacgtt tgagaaaaga aaggaaaatc ctcagtgaat aaagaagaaa tagttgaaag
    84241 cttagccacg attactatat tgtttgatga accattgatg aactatacgt ttacgaaaac
    84301 aggtggacca gctgatgtcc ttgcattccc gaaaaaacaa gaagaagtaa agcaaatcat
    84361 cgattattgc cgaatccatg atataccttg gatggtatta ggaaatgcaa gcaatctgat
    84421 cgttcaagat ggcgggattc gcggtgtagt catcatgctg actgaaatga aacagattca
    84481 tgtcaaagga acgatggtca ttgcggaagc aggtgcctca ctgattgata cgacttatgc
    84541 agctttggca gaatcattga ctggatttga atttgcttgc ggcatcccgg gaagtgtcgg
    84601 tggagcggtt tatatgaatg caggtgcgta tggcggagag atcaaagatg tttttgctga
    84661 agtggatctt ttgcttgaag atggaacatt gaaaactttg acaaaagacg aaatggtatt
    84721 ttcttatcgt cacagtaaag tccaagaatt acgtgctatc gtattggaag ctcgattcag
    84781 tttgcaaaca ggggattatg aagcaatcaa agcaagaatg gatgagttga ccgaattgcg
    84841 tcaatcaaaa cagccattag aatacccttc atgcggaagt gtctttaagc gcccagtagg
    84901 acactataca ggtcagctga tccaacaagc aggattgcaa ggcctgaaat ggggcggtgc
    84961 acaagtctct gaaaaacatg ctggattcat cgtcaatatc gatcatgcga cagcgacaga
    85021 ttatattgaa ttgattgcgc acatccaaga agtaatcaaa gaaaaatttg atgtgtcttt
    85081 agaaacagaa gtgcggatca ttggagaaaa acaagcaata tcagaaacaa gcagaagttt
    85141 agccaattaa taagtaatgg aacttaaaat aagtaaaaga gcaggacggt ggccgtcctg
    85201 ctcttttttg tggttgttta cgcaaatggc gcaaatcttg tacattttaa cacaataaaa
    85261 caaaaaatat agaatatttc ctcataaatt tttccatttt ttgcaatatt ctcaaaaaaa
    85321 agataaaaaa tgagaataac tgatgaaaaa acaagcataa tccatcataa aatcagtata
    85381 ggtgaaaaga tgaactttca tctgcatatc aagtaattgg tatgaggcat tttttgaggt
    85441 gtaggatatg aagttggatt accttataga agaggaagat agagaaaaac tgctgctagc
    85501 tagatattta gaatttgaga aaaagaatta tgtagtgctg cctaagttat atgaaacatt
    85561 aggtttgtct aaatttaaaa caagaaatta tatcatagaa ttaaataaag aatttactga
    85621 attcaaaaca aagccagaga tccaaatttc agaagaagca gagatccatg tcgaacgtct
    85681 gaatttgacg gatatcaaaa atttaagaag aatctacttt caacgatcaa aaattgctcg
    85741 actgttaacg gagatcgtgg attcaaaagt aacgatcgaa aaatttgcag agaatcattt
    85801 tttaagcata tcaagagcct atgtaaaaag aagagaatta gtctcttttc ttgaacagca
    85861 tcaaataaag ttaaagaaaa acatattagt tggggaagaa ataaacatac ggaacgtatt
    85921 atttggggta tatttcgggg tctttaatgg tttttctatg ccatttagag aaagcgtcat
    85981 gaatgaagca aagagtatcg tgcaatattt tgaatatctg ttcaatttaa aactgtctgc
    86041 aacaaaacga atcaaattca atttgctaac cactattagt ttgttgaggg cctatgggaa
    86101 tcaaccaatc ggaaaacgtt tcttttcaga tgaatacttt aagagctcag aaggaatgaa
    86161 gatcatcaag gatttatcac atatttcttt tcttcatatc cctaaagagc agatcattga
    86221 tgagatttct tatatcatgt tgtttttgat cacggaagaa gaatttgaat tagatggcgc
    86281 atttggactt gcggaagctc attttcatga tatccacatg attagtcaaa aaatgtcagt
    86341 ggaattgatt gatcaattac cattttcgat tgaaatctct gaagaagaaa gactatatat
    86401 acgaaaagaa cttcgtatgg gtatgatgca tgtcaatcga aaaaattgga tattcgactt
    86461 tgagatgact agctttacgt ctaaaaagca gctacagttt ttcttcgaga cgtatccaat
    86521 attcagtaca gcaatccaaa aggtcataca agagaataaa cgctatttct tgggaaaaga
    86581 tgaacgtttc atgattcgct tattttatga ttatctcttt ttattggtcg gtatttgtcc
    86641 ggtatcatat tttgaagcac cgattcatgt ctgtattgat ttttcgcaag gatctgctta
    86701 taccgattac attatttctc aagtgaaagg atttaaaaat tacaatattg tgattgagag
    86761 tcgaatcact tctcgtacga atatttattt atctgattgt ttacttgaaa aatttaataa
    86821 agaacagata atttggaaaa atccaccgac tccagatgac tgggaatatt ttggaaatac
    86881 catcatcaga gaaaaaaata gacatttaac tgaaaaaatc agctagtatt tatggatggc
    86941 agctttgtct cacatattta ttagctgatt tatgtttttt tcaaagaaaa ggtaactaga
    87001 tataactaga tataactaga tatattactt aacaaaatta aaaagtacat aagttattat
    87061 tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact ctcctaatag tccatcttct
    87121 tttgatttta tttatataat gaaatcatct tatgaaaatt actacttcat gtttagacaa
    87181 gagtagggag gactaagaat aaatgtgttc aaaaagacgt tcaaaacaac taaataaaca
    87241 cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa cactacgtta agtttatgat
    87301 gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg catgtacccg ttcaggcaac
    87361 gacacaaacc atcaaggtcg tacgtacctc tgtttctgag accactgatt cccctatcac
    87421 tattactcat actttcactg atgcaaatgg aaaacagcta cttcctgcat tgacttccta
    87481 taataaagtc agttatcctc caacgataaa ccactactgg ttaattccta acaaaacatt
    87541 atggacatcg gaaaccggaa gcagtgtgcc gctttctatc ttttccgata tgactgatcc
    87601 tacaaaaggt gatcagttaa aacagatcac tgatattttg aatcatgctt tgacagatat
    87661 aaaggacggt ggaagcgcac aagtttctta tgtatatgaa aaagattatt cacagtttga
    87721 aagaaagaaa atcgatgttc tggcagggac aaaagtttcg atatctgatc tggtagaaag
    87781 gctgaagaac gcagatggaa cacaaggaga tatgtccaaa gtaatttcaa acaatgggaa
    87841 tatcgtggat acgactaata caggaacatc tactatttcc ttgtcctatt ttgatccaat
    87901 tgctttaaaa acaatgactg catctgctgt gatccatgtt cgtgctgatc aaacagctat
    87961 caaaggaaaa gatcttcact taagaacagg agagagttac gcccctaaag atcttgttga
    88021 atatgtaaca aaaagcgatg gaacttcagg aaataaagaa gaaatcaaaa tgaacgggac
    88081 agtagatact acaactccgg gagaatatcc cgttgtatta acttatatag actcgaaaac
    88141 atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa gcatctgctg cttcatcgac
    88201 tgtcatcact cattttatag atagtcaaac aaataaagaa cttcaagcag aaacacatat
    88261 gagtagtgta aatcgtattc ctgatttgcg cctacctgct gaattttctg atcgagtgcc
    88321 aaatatagat ttatcgactt atgaacaaaa tgggaaagtc acttctattt ctgattggat
    88381 gcaagcaaac ggattagaca gaaaaagtca ctggtcggag atccttgaag tacttcactc
    88441 agaactgatg aataatagga agcaatcagg aggggaaatt acctttactt atgtttatat
    88501 accagacaat cgttcattag aaggtcatga tcttattgtt catccaaacg aaaaaataaa
    88561 caaagaagag ttgatccaat cagcaacaaa ttccttaggt aaacccgtac cgttaaagga
    88621 tgtatcggtt acaatcaatg aagtaccatt ggaggatggt tatttgacca aacaaacagg
    88681 tgattttacg attatgtata gttatcatga cccgtattca ctccaagaac tccatgctca
    88741 gtcactatta catgttttac cattaaacaa acagatagat caaaaagaac aaaaaagacg
    88801 agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat gcagtttctc caacagggaa
    88861 agtaaaacaa acaagaagcc tagagttagc aagaatatct agaaaaatct cagaatctaa
    88921 taaaacgata aggcaagaag ctcatttggc cccacaagtt tctggattgg aaaatgaaat
    88981 tttagagcct aaaataaaac aacaatcaga aaaagaattt gttgctaaac agtcggaaga
    89041 tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa cgcttgtcaa ctattgattc
    89101 caccgatgag catctgcagc cgcaagtacc acttcctagt ccgggaacac ccgcacctgg
    89161 tggaggtatt ccttcttctg ctttaggaga tttcatgcga ggtatggcag gaacatgggt
    89221 atacgctaat cgaaatgaat ttgacgcaga tgactggttc tgatttatat ctctatctgt
    89281 tatagtcaaa taagtagctg agtcatatgt tatttggcat aatggagacg aggagggata
    89341 gaaaaatgtt agataaaacg ttaccttacg cagaaatctg gatgacaaga ccacttcaac
    89401 aaaatgttcc aatcacgcct ttagcggaag gttttgaatt tcgattttat caaaaagaag
    89461 atgaatctgc ttgggcggag attgagactt ccgttgggga atttcaaacg acagatgaag
    89521 cacttgagta ttttcaacaa aactttgctc cttatcctga agaacttgaa agaaggatgt
    89581 tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg taccgcttgg cgaaaaaaat
    89641 caagagagca agtgtatccg gtttttcatt ggttagcagt gaaaccagat taccaaggaa
    89701 aagggctcgc aaaagcattg actgccagag tcttgcaaga atttcctacc ctccatacgg
    89761 acggaccaat ctatcttcac acacagacat ggagccatca ggcgattgct ctttataaaa
    89821 aaatgggttt tacttttatt gctgaaaatc tagacggcac acctaatcca gattatgaaa
    89881 aagttatgca ggttttaagt aaattagata aataacgaaa tggtgaaata agtcctcagt
    89941 attgggataa aaaacgaaat agtcattcct catatcttac ttttttgagt taatactatc
    90001 ggataactaa tggcatgcca tacattcaaa aaagaagggg cagtgacaaa agtattgtca
    90061 ctgccccttc tcttgttttt tagcatgttt tatcgtttga tttttttagg aataaaattc
    90121 ttgatgaacg taaatttttg gtaatcgttt cgactataga ggtaaccaaa tacactgaaa
    90181 acaactgctc caataaaatt aacaaataaa tccttcatag tatccatgat tcccacgtct
    90241 aagtaaccat tttggatgat tgttttatct gttttaccat ctgcattttg actttcaatg
    90301 attgtccgag tgatatctgg aacatgatag acagtgtttc cgtctttact taactccaca
    90361 ctattgattt gctggatgat cgtatctttt tgcatatcga attgcagtaa tttatctgct
    90421 gaaaattcca caaactccca cataacacca actgtcatcg aaaaacagaa ggaaacaact
    90481 gctagtaata aaggagtcaa gttcatggtt tttgtatttt tattgagcaa ataaacaagg
    90541 gaaaagccga cacccccagc taaaaatccg ctgaatgcat gtagaaaagt atcccacatt
    90601 gaaaaatagc cataaaaatc acttaactct cctaaaactg ttgaactaaa aataaaagat
    90661 aacaaaatga tttctagcat atttggaatc tgtatcttta atatcttttc cagaaaataa
    90721 gggatttcaa ataaaattaa tgtcagtaat aaaagcattg tatttcccca gtgttggttc
    90781 cagacctgat ccactagcat aataacgacg atgatacgta ataaaatata gattccgttt
    90841 gtgattctac ctgcagtttt tgtactgttc gtaaaaaagg ttttcaaatg attcataaaa
    90901 taactccttc atgtatatcc ttaattatac taagaagcgt gtgtttacca aagctatatg
    90961 acttaaagaa aaacagagtt gatattgtga aaaaaatcac ttaatatagt ataaggagaa
    91021 aagggggata caaatgaaaa aattagttgc tggtttatta acagccgttg cacttcttgc
    91081 tatgagcgca tgcggcgcag atcagaaaaa tacagcgcag acaagttcta gtacgaagga
    91141 atctgccgaa gtatcatcag gcgcttctga acaagtatat acagatccaa aagaaatgaa
    91201 agaatcttat gatgtcgtga ttattggagc tggtggtgca gggatggcag cagctatcga
    91261 agcaaaagat gctgggttga atccagtaat tttggaaaag atgcctgttg ccggaggaaa
    91321 cacatctaaa tcatcaagcg gtatgaatgc ttcagaaaca aaatttcaaa aagaagaagg
    91381 aatcaaagac tcgaatgatg ccttctatga agaaacgcta aaaggtggtg gaggaacgaa
    91441 tgataaagaa atgcttcgat actttgtcga tcactctgct gatgcggtcg attggctaga
    91501 tcaaaacggt atcaaactgg acaacttaac gatcactgga gggatgagtg aaaagaggac
    91561 acatcgtccg tctgatggct cggctattgg tggatactta gtggaggggt tattgaataa
    91621 cgtccatgag cgggaaatcc ctatttttgt caacgcagat gtgacagaca ttaagaaaaa
    91681 tggggaaacg gtcaatgaag tgactgtaca tgttcaagga gaagaaccaa aagaggtcac
    91741 cggaaaagca atcgtagtta ccacaggcgg ttttggtgcc agcaaagaat ttattgaaaa
    91801 atatcgtccc gatttagctg attatattac gacaaatcaa gaaggatcaa ctggagatgg
    91861 tatcaccatg attgaaaaat taggtggaca aaccgttgat atggataaaa tacaaattca
    91921 tccaactgtg cagcaggatg agggggtctt gattggtgag gttgttcgcg gagaaggagc
    91981 aatcttggtc gatcaagagg ggaaaagatt tgtcaatgaa atggatacaa gggataaagt
    92041 atctgcagca attacagcat tgcctgaaaa atcagcttat ttgatttttg accaaggcgt
    92101 gcgtgatcgt gccacagcta ttgaatttta tgaccaaaaa ggatatgtca caactggaaa
    92161 gacaatggaa gaattagcag aaaaaatcga tttaccaaaa gaaacactag caaaaacagt
    92221 tgatggatgg aatacggatg ttgcggagaa aaaagatgct caattcaacc gtgccacagc
    92281 catggaacat gacttatcgt ctccgaatta ttatgcgatc aaaatcgcac caggaatcca
    92341 ttacacgatg ggcggtgtga aaatcaatac gaacactgaa gtattagata aagaaaacca
    92401 gccaattagg gggttatatg ctgctggtga agtcacaggt ggcttacatg gaaacaaccg
    92461 gatcggtgga aattcagttg cggagatcat tatttttggg cgacaagcgg gcatacaagc
    92521 agctcgattt gcttcggaaa atgaatgaaa tagaacgtaa ataaaaagag gttgtgaaat
    92581 cagcgttttg agctaatacc gagggtcagc tgattgaaca ctgtttagtg aagaggttgt
    92641 gaaaaagctg ccctgcatca agtaataaga acagccaagt aaaaacagct tcttatgttt
    92701 ttaacttggt tggacttatt accgcagatg caagcttttg aacaccgttt attcggaaaa
    92761 agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
    92821 ggaagcagaa gaaattcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt
    92881 ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttgat
    92941 cagcatgatt ttattcagtt atcttcaaaa tgatttttcc gatgtttttg tttgcttcca
    93001 tatattcatg cgcttgtttg actgcgctga attggaatgt acggtcaatg attggccgaa
    93061 tcttgccatt ttctagaaaa ggcccagctt tttgcataaa agtttgtgcg agctctgctt
    93121 tatactgatc acttctaggt gtcaataatg taccaagcag ttggacacgt ttcccaatca
    93181 atacaccaag gtctatctca ggcacgatgg tcccacctag cataccaatc aagacccaac
    93241 gtccatcaac ggctatagaa gctaaattct tttcccagta agaagcgccg atgaaatcca
    93301 aaataacatc gactccccga ccttcagtga tttttcgtac ctcttcttga aagtcttgtt
    93361 ttttatagtt gatcaattca tctgcaccaa gttctttaca aaaggcgagt ttttcttctg
    93421 atcctgcagt aacgatgatt ttcgcgtgag aaagctgtct agctaattgg atcgctgctg
    93481 ttcccacacc acttgctcct gcatggatca agacagtttc gccttcttgt aattttccaa
    93541 gccagtaaag tgtttgataa gcagtcaaaa aaacttctga gattccagca gcagaaacat
    93601 agtctagcgt atcagggaga aggattgcac gatcggcagg catcacagca tattcggcat
    93661 aaccacctag attgactaat ccgtatactt ttgtcccagg agtaaaagtg gagatatcac
    93721 ttttattctc aactacctct ccagctactt cgactcccaa aatagggtag ggaggtttca
    93781 aagagactga ttctcttttc ataatatctg ttcgattgac agctgctgta tggactttga
    93841 tcagtaattc gccttgtttt ggtttaggaa caggcacttc aatttgttct aatacatcta
    93901 cagaaccagg atgcttgatt gaaatggcac gcatggatct attcctcctt tgcccattct
    93961 tggaatgctg tattcaactc tttgctacct tcagaaagta ctagagtcgt acgagaaata
    94021 tccgtttctt ccactagagc ggcaagtaca gcagcaactg tagagcgagt gatcgttttt
    94081 gatgccttct cattaggctt agttaattga atggaagtaa cttcttcatc gttagtcaat
    94141 gtgactggtt gaagaatgac aaaatctaag ttagttcgtt tcagccattc atctgcataa
    94201 tgtttagtaa tataataatc tgtcatggac tcaggccatt ttgctggttc atctgcataa
    94261 acagcactga ccatcagata gcgagagaca tgcgcatttt cagctgcgat cactgttttg
    94321 attgcgccat ccaagtctac ttgcagaaga ttctttcctt gagagccggc agcaaaaatc
    94381 aatacatcga tgcctttgaa tgcttcggtc attttttcca cggaccaagt taaatcaaaa
    94441 gagacatacg aaacattctc tttttttact aatgtttggg aagcaacatc tctcacccct
    94501 gcaaaaattt catgggaact tggagctaaa tcttttatca ggtgtcggcc aatttgtccg
    94561 tttgcaccta caacaaaaat tttcatttgg atcatccttt cgacgtcaaa tctttccatt
    94621 caatagtaac accaaaagag aaaaaaatca gagatcggct acgatgacta tcagttagta
    94681 agagaagggt gcttgaagta tcttaacaag acattgcata ctgctgcagt cgcaagacct
    94741 gcgacgattc cttcaggtat accacttgta aaaataaccg ctagaatagc tgaataaaca
    94801 gcatttaaat ttgttccgat gactttggca taatcttgct ggaataaaaa gtagatcata
    94861 ttcatcacta aaatcgtatt ggtcaatgaa ccggaaagtc cagctaagaa taaagaaacg
    94921 gaatctgctt tttgtttcat tagtttgcgc agacctttat aaacgaaata aggaaccacc
    94981 ccaatcaaga tgcgaggaat aaacgcgatc agcaaggctt tccagcttcc atgttctgtt
    95041 ccgatgactg ggataaacgg agaaaagaca aaagacaaag gtgtttggat aactgtgcta
    95101 cggatcatgc tgatcagtcc aaatgtaccg ccaagaaaag cacctagtct tggtcctaaa
    95161 acgatcgaag caatgattac aggaatatgc atggttgttg cattgattgg accgataggg
    95221 ataaaaccta aaggcgtaac agctaataaa atcaaaatag ctaaaaacat cgcagttaat
    95281 gtaaagtttt ttgtattttt cataagtact ccttttttct aagcatttct ttcattaaga
    95341 gtcagcatga cctgttcgat gatcgtctga atatcagcta aggcaccttt tccaaaatct
    95401 ccacaagcta aaagagattc tcttggttca atttcctgat agccgacaga tctcaatgtt
    95461 tccagattac gctgagtgat cgggttttga tacatattcg tgttcatggc aggggcaatc
    95521 aattttggtg ttcccactgg aagcgccatt gcgactgttg aaagaagatc gtctgcaatg
    95581 ccgtttgcta gcttgccgat aatgtttgct gtagcaggcg cgatgagaaa caagtcagct
    95641 tgttttgcta gttcgatatg attgattacc tgcggatttg attcttgcat gacgtctgta
    95701 tggactgcgc gcttcgacag agattgcaag gttaatggcg tgataaaatg tgtgctgctt
    95761 ttagacataa tcacatctac ctgggcacct aatttgacga attgacttgt aagatcagcg
    95821 cttttgtaag cagaaatact tccagtaacg cctagtaaga tctttttcat tttgattcct
    95881 ccagtctaat tttctcagta atcaatgcag caatctcaga tttactttga gcttcttcca
    95941 ctgttccatc tttagataag agatagccgt ggtgtgtctc gccatggatt tccgtcaaat
    96001 catttgcgaa aataaaatca gttttgttat ttatcagact attttttgcg actgccagaa
    96061 gagattcctt tggtacatct actaatagtt tgaaaccaat gatttttgct tttggctgcc
    96121 attcacgtag ataagaaatg atttttggtg ttttcttcaa aacgagcaca agatgctcgg
    96181 tgttagaaga gatctttgaa acagtggtat ctgtctgttc attttccgca aaccaattgt
    96241 ccagttcttc ttgcgttgaa gaagtgggta ggttttttgc cagctgttct tctgaaaaac
    96301 taaaagcagg agtaaaatca ctgactgcca tactgtggat aatcgcatcg tagtgttctt
    96361 ttttcgttag ttcttctaat tgtaaaaata agtccagtgc agattcgatc gtataaatcg
    96421 ttatattgga gtgtcttttc ggtttcagtg cttgtttcgt agtcacgtaa tcgattgtgg
    96481 tatccggatt ggcagctaaa taatcagcta atgcttgccc taatcgtcct gtcgaatgat
    96541 ttgtaatagc acgtacttga tcgatttttt cagtcgttcc tccagctgtg attagaatct
    96601 tcatttatgg tcacctacat ttactagtgt tcttccttgg tgtgttccag caagtagttt
    96661 agatgctgtt tcaggcaatt gatccagagt aatctcttgg acgaccagtt catctgcaat
    96721 ctgcaaatcc gccatctgtt gccataaaga aagacgttga tctattggta cattgacaga
    96781 atcaattcca atcaactgga tgtttcgcag aataaaaggc aagacagtcg cgtttagttt
    96841 gattccccca gcattgccac ataaagctac tgcaccgtta taagagatca atggaagaag
    96901 actgcttagc tgctcgccac ctactgtgtc gatgacatag tcgatttgct gttttcctaa
    96961 agcttttgtt gtctctggca gaaattcatc aggtcgtacg acttgcaaag cacctagact
    97021 ttttagccat gtgacagact cttcttttct ggaaaaggca atgattgact ggaatcctag
    97081 tttatgtaac agggctatcg ctgtactgcc tacaccgcca gaagcaccag taacgacaac
    97141 ttttttatcg gaggtcatac cttgacggat taaagcgttc acacacaaca tagcagtaaa
    97201 ccctgctgta ccaaggatca tcgcttgacg cagcgtcata ttttttggta gaggaactaa
    97261 ccagtctcct ggtacttttt gatattggct ataaccgcct ggatgggtga cgccaagccc
    97321 aaaaccagta gcaagtacaa gatcttcttt tgaaaaacgt ggattgttac tttggacgac
    97381 agttccggat aaatcaatcc ctggagtcat cggatatgtt cgtatcacct gaccagactc
    97441 gctcattgcc aatgcatctt tataattgac atcagaatag gcaattttta ccaagacttc
    97501 attttcagcc aactcagtaa atggcagatc actgattgct gtatggaaaa caggttcctt
    97561 tattacctga aaagatcgaa aagcgttcat tcgtgagaca ctccttttaa aattttcttt
    97621 tatcatacca taaagcctac tcttttattc ttgttcttcc gtaaatttag acaaaaatta
    97681 ggtgaaaaat aaagaatcaa acaactagaa tttattccaa aagataaaga aatattcagc
    97741 aaaaaaaatt ttacaaaggg ttgccttttt caataaattt cgtatactaa tacgagtggt
    97801 agtaaacagg aagtttacta ttagaaaact tcgattcgga ggaaattatg gaaattggtg
    97861 agaagttaag aaatttgcgc atccaaaaga atttgacaca agaagaacta ggcgaacgca
    97921 cagatttgtc aaaagggtat atttcccagt tggaacgaga tttaagttca ccatcaatgg
    97981 aaacattctt ttctattctt gaagtcttag gtgtgactcc agaagaattt tttcatcaag
    98041 aagcagcgaa tctgcaagtg gtttattcaa aagaagatca tacggtttat ttcgatgaag
    98101 aaaacgggta tgaattggaa tggctcgtgg ctgattcgaa tgaaaaagaa atggagcctg
    98161 tcttgctgac ttttgaaggt gcaggagaat ataagacgtt tgaaccttca ttatctgaaa
    98221 cgtttatttt tgtattagaa ggggaattag agttaagctt aggagagacg gtctatgtgg
    98281 caaaagcagg acagtcgatc tattatcaag cgacaaaaca acatcaatta aggaatcatc
    98341 tcaaaaaacg aacaaaagta ttgattgttg caacgcaatc atatttgtaa tatagggggt
    98401 aaaaacgtgg ggaaaccaat catttcattt gatcaagtag tgaaacgcta tgatgatgaa
    98461 acagtattaa aaaaagtaag ttttgagatc gaacaaggaa aattttatac actgcttggt
    98521 ccttccggct gtggtaaaac aacgatccta cgtattatag ctggattcac cgaagctaca
    98581 gaaggggaca tctattttga agggaaacgg ttgaatgatt tgccagctaa taagcgacaa
    98641 gtaaatacag tcttccaaga ttatgcactt tttcctcata tgaatgtttt tgataacgtt
    98701 gcttttggat tgaagatcaa gaaaatgtcc aaatcagata tcgaaaagaa ggtaaaagat
    98761 gctttgcgta tggtccagct tcctggatac gaaaaacggg agattagtga aatgtctggt
    98821 ggtcaaagac agcgtgtagc gattgcacga gcaattgtaa atgaaccgaa agtattgcta
    98881 ttagatgaac cgttatcggc gcttgatttg aaactacgta cagatatgca gtatgaactg
    98941 cgggatctgc aacaacgatt aggaatcacg tttatttttg ttactcatga ccaagaagaa
    99001 gcgttggcaa tgagtgatga gatttttgtc atgaataaag ggaaaatcgt ccaaagtggt
    99061 acacccgtag atatttatga tgagccaatc aatcatttcg ttgctgattt tgttggggag
    99121 agcaatatcg tggacggtgt aatgatcgaa gataatctag ttgagtttgt tggtaagcaa
    99181 tttgaatgtg tcgatggcgg gatgcgtcta aatgaaccag ttgaagttgt tttacgccca
    99241 gaggatttga cgatcacgac accagataaa ggaaaactag tcgtaacggt cgatactcag
    99301 ctgtttcgtg gtgtccatta tgaaatcatc tgttatgatg aacagcaaaa tgaatggatg
    99361 gtccattcaa cgaaaaaagc taaagaagga agcaaagtag gattggcctt tgagccagaa
    99421 gatatccatg tcatgcgttt caacgagtct gaagaagaat tcgatgcacg tcttgacagc
    99481 tacgaagaat aggggggaca agaatgaaaa caatgcgcag gatctattcc gtcccttata
    99541 ttttgtggct cggtttattc gtgatcgcgc ctgtattaat gatcatttat caatcttttt
    99601 ttgatatgaa tgggcaattc acgctgaata attatctatc ttattttacg tcggggacat
    99661 cgctttctat gacgatcaat tctgtatggt atgcattttt gatcacgttt ttcacgctga
    99721 tcatcagtta cccgactgct tattttttaa caaaactgaa gcacaagcaa ttatggctga
    99781 tgctgattat tttgcctaca tgggtgaatc tgctgcttaa agcttacgca tttattggga
    99841 ttttcagtgt gcacggcagc gtgaatcatt tctttgattt tatcgggatc gggccgaaac
    99901 agatattatt cacagacttc agttttctgt tcgtggcaac gtatatcgag attccattta
    99961 tgattatgcc aattttcaat gcattggaag agatgaatcc ttctttgatc agtgctagta
   100021 gagatttagg cgcgaatagc tttgagacct tccgacgagt gatttttccg ttatcattaa
   100081 atggtgtgaa aagtggtgtc caagcagtat ttattccttc tctttcttta ttcatgctga
   100141 ctcgtctgat tggcggaaat cgggtgatca ctttaggaac ggctatcgaa cagcatttct
   100201 tggttacaca aaactggggg atgggttcta caatcggtgt gattttaatc attgctatgt
   100261 tcatcgtgat gctcctgaca ggcgagaaga aaaaaggagg tcgctcataa atgaaaaaat
   100321 ttaaatggtc gtatctttat ttagggatcg tctttttatt cttatatgca ccgatcttct
   100381 atctgatttt ttattccttc aatgaaggag gaacgatgaa cagctttaca ggatttactt
   100441 gggaaaatta tgaagctgtt tttgaagata cacgtttgat cattatcgta ttgaatacgt
   100501 tcatgctggc tttcttatct gcattgatcg ccacgatcat cgggacattt ggggctatgg
   100561 gaatctatta cacgaaaaaa cgacgtgcac ggacagcgtt gttgagcatg aataacatct
   100621 tattagtctc tcctgatgtt attatcggtg ctagtttctt gattttcttt acactactag
   100681 gattcggatt aggttttcag agtgtattgc tttcccatat tgctttcagc attccaatcg
   100741 tcgtcttgat ggtcttgcca aaattgcagg aaatgaacga ttcaatggtc gatgcggcta
   100801 gagaccttgg tgctaataat ttccaagtag tcaaaaatat cattttgcct tatctttctc
   100861 caggaatcat agctggatat tttatggcat ttacctattc tcttgatgat tttgcggtta
   100921 catttttcgt cacaggaaat ggctttacga cgctctcagt agagatctat tctcgtgcaa
   100981 gacaaggaat cagtttagaa atcaatgcgc taagtgcgtt agttttcttg ttctccatga
   101041 tattggtcat tggatactac tttattagta aagaaaaccg tccaaaacgg ctgaagaaaa
   101101 acagaagagt acgcgagggg gtggcacggc tgcgatgaaa aaattacaat ctctgttttt
   101161 aggtattctc atcattattg cggtgctgct gcttggtgtt cgtcaacttg aaaaaacaag
   101221 tggcatgagt ggagcaaagg tacttaccat ctataactgg ggagattata tcgatcctag
   101281 tttgatcacg aaatttgaaa aagaatatgg atacaaagtg aattatgaaa catttgattc
   101341 taatgaagcg atgttcacaa aaatccagca aggtgggaca aactacgaca tcgctatacc
   101401 aagtgaatat atgatccaaa aaatgatcaa agaaaaacta gtacttccat tagatcattc
   101461 gaagatcaaa ggattaaata atattgatcc acgattttta gacttggatt ttgatccaga
   101521 taatcagtat tcgattccat atttttgggg aactttaggg atcgtatata acgataaatt
   101581 ctttgatgca aatcaaatta agcattggga tgatttgtgg aagccggaat taaaagacag
   101641 tttgatgctg attgacggtg cacgagaagt tatggggctt tctttgaata gtttaggcta
   101701 ttctttgaat agtaagaata tggcacagtt gacagaagca gcaaataaat tgaatcattt
   101761 aacaccaaat gtcaaagcaa tcgtggcaga tgagatcaaa atgtatatga tcaatgaaga
   101821 agcctccgtg gcagtgacat tttctggcga agctgccgat atgatgtggg aaaatgaaca
   101881 tttacattat gtgatcccac cagaaggatc caatctttgg tttgataata tcgttatgcc
   101941 aaaaaccgtg aaaaataaag aaggtgccta tgatttcatc aactttatgc tagaaccgga
   102001 aaatgcggca caaaatgcag agtatattgg ttattctaca ccaaatgaag aagcaaaagc
   102061 gttattgcca aaagacattt cgtcggacga acaattctat ccatctgatg acacgatctc
   102121 ccatcttgaa gtctacaaag atctaggatc gaaataccta ggcatctata atgacctatt
   102181 cttagaattc aagatgtata ggagataatg gcaaaactca gtaagaatgg ggacttatca
   102241 acgttctaaa gagctaaaaa tcgtcctttt tctatctgag ttttccaatc ttctaccaaa
   102301 agcggatgaa acgccgcttt ttttgtgtta ttttattttt tctaccacgt ttttctacca
   102361 gagaaaagtg gacaaaacca ttgtaaaata gggcccgaga cgggctagta aatctaagat
   102421 tgaaaaagtt acagaaggat aaagtaattg gaaactatgc aaatatactt aacccttctg
   102481 taatctatta ataaaactaa tatatgaatc cagttcaata tttgtagcta tgaaaaaaca
   102541 taaaaatcat attagataaa aactaaattg gtagataaat tattttgtca gatatctgag
   102601 gcaaatattc gactatttgt gtttcaaagt aaaaattgga atacaaatat agaatagaca
   102661 tattgcagcc gatagtttat tatgctttta gacacattaa actggaggaa aaaatgaaaa
   102721 agagcgctaa agatcgagtt tttgatgagc tgatacgact aacagggaat atgtcttctg
   102781 tcactgccga gaaaatttcg aagaatctag gtatctctcg tcagaatgcc agccattatt
   102841 tgacgagatt agtggaagat aaaaaggtcg agaaattacc gggaaagcct gttttgtgga
   102901 agcctttaga tgaatatgcg gttgtagata atacggagca aataaacgaa gcctttcatt
   102961 ctgtagttgg tcatgacgga agtctgagag aggtcattca aaaatgtata gccgctgtca
   103021 aatatccgcc taacggactc agtgtattaa taaatggagc cacgggtgtt ggaaaaagtt
   103081 ttttagccac aaaaattttt gaatacgcta tccatgagca aattattgaa aaagatgcac
   103141 catttgctat cttaaactgt gccgattatg cggacaatcc cgaactgctt tcggcaacac
   103201 tgtttggata caagaagggg gcctttacgg gggctgaaaa ggataccgat ggtttattgg
   103261 caacggccaa caacggctat ctttttctcg atgaggtcca tcgcctatcg aaagaaaatc
   103321 aagaaaagct ctttctattt attgatactg gaaactatcg tccagtcggt gaaaatgtaa
   103381 attggcattc tgctaaggtt cgttttattt tcgccacgac ggaaaaaggt gaaaattatt
   103441 tattagacac ctttgatcgg cgtattcaaa ttagtgttat gttgccaact tttgaagatc
   103501 gccccattcg agaacgatta gagctgattc aattattttt tcaaaatgaa gcggatgttt
   103561 tacaaaaaga tattattgtt tcaagagaag ccttgtcttt aatgttatct catcccttta
   103621 gtgggaatat tggtaagctg aagaatatca ttaaaatcag ttgtgcggat gtgtatagtc
   103681 gaacacaaga agaaccatta acgattggga aaaatgagat cttgattcag ttaaatatgc
   103741 tggaatccga agtagaaagc ttgccgattg ctagtttatt gataaagaaa aatcaaaaga
   103801 taaaaacgga tcgaatcaaa ccgtttgatt atgattcgat tttggaaaaa attactcagc
   103861 tgataaatga aggtaatttc aatgaaaatt tttcttcgat aaagaatgaa gtacagcatt
   103921 tggtgcatca cattgataag gattcttttt tatcaatgag tagaaaattg atgattcaga
   103981 tgtttagaaa ggtctgggta tccgttttag gaaaaacgta cggattgacg acgagtggac
   104041 ctgttgcaga aattattagc agatcctatg tcatttcccc aagctataag acagacttaa
   104101 ccgggataaa tcgtacatta gctgcaaatc tacctcggac gacttactta tgtaatcagt
   104161 tcttggagca tttaccaccg ctctcagaaa aggacgagaa ctttctaaga ctgcttattg
   104221 tcgtgacttt atctgattat gtagatgaat ccatcgagct gaagggcctg ttgcttgctc
   104281 acggagatag tacggctagc agcattcaag cagtagtgaa tcaattatgc ggcaattatg
   104341 tttttgaagc gttggatatg ccagtagaga gtaatgtgtc agagattgtc gaatcgacaa
   104401 ggaatttcgt gaaacggcaa gtgaatacag atagtctagt gctgatcgta gatatgggct
   104461 ctttaaatca aatttacaca cagatcaaga acgatttaaa aggggagtta cttcttctga
   104521 ataatttgac gacttccatt gcacttgata ttggcttgaa gatgacgatg aatgcgccat
   104581 ttaaacagat tgcggaggat gcagcagaca agtattcaat caatgttcaa tactttgaag
   104641 gcttttctca aagtagcaac attatcattt cctgtatgtc gggtttgggg atttcggaca
   104701 aattgaaaga tgtttttcgt catcatttat cggatgaatc catcgaagtt ttcacgaagg
   104761 actacagaga actacgtgaa ttaatcgacc aaaatgatga aacttatttt gacaagacaa
   104821 aattggtcat cacgacttcg gatcttccac gttccttttc aattcctaat attaatgttt
   104881 atgacatctt agacggtgat ggagcagcca atttggagca tattttgagt cctgaaatga
   104941 agccggcaga atttaaccgc ttgattcagg agctagtaaa attcttttcg ttggaaggaa
   105001 ttgcggaccg tttgaacttt ctgaacccaa cgatcgtgat gagtgaagtg gaaacagtca
   105061 tttctaaata tgaaaattac tatcatttca ctttaaatgg ccgtgtaaaa ctaaatctct
   105121 atatgcatac ggcattgatg atggaacgtt tattcttggc tcggacagaa aaatctgaag
   105181 aaccgaatta tgatttgaaa ccagatgaag atgaatttta ttatgtttct agaagtattt
   105241 ttcaatcaat ggagatgaaa tataatttta aagtcaatgc ttatgagctt tcgctgctct
   105301 atgaattatt aggaccgtat attcaaaaat aaagtgtcta aaaaagtgtc taatctgttt
   105361 tttttaggtt agacactttt tttaatgtga attaacgtga ttaaaccaat gtttttaaaa
   105421 gttggcacag gaattgctta tataaaagtg aacagaaaga aaggaattaa ccatatgaag
   105481 aaaattctaa tagcgactca cggtcactta gcttcaggct ttcttagcag tattcaatta
   105541 ctgacgggga aggttcaaga gattacggtg attaatgctt atgtggatga gtgtgatttt
   105601 gaacaggagt tgaatgtatt tgtaaaccag gtgaacgagc aggatcagat ttttgttttt
   105661 accgatttat ttggcggaag tgtcaatcag aagattacca aaaccttttt ggagaaagag
   105721 attgctgtaa ctattttgtc tggctttaat ctgccaattc tgctcgaaat agtattggca
   105781 accaaggaat taacaaagga ggatattcag gaactggtaa ataaatgcca acaggaatta
   105841 aagatgacca ttcttgatga ggtacatgta gtaaatgaag aggaagattt tttttaggag
   105901 gaagaaaaat gattaaagca gtgcgaattg atgaacgatt aattcatgga caagtagcga
   105961 tgacttggac aaagagtcta aatttaaccg ggttagtagt agcaagcgat gaggcggcta
   106021 ccaacgatat tcaaaagatg acattgaaga tggctgcacc atcgaatgtc aaagtcatta
   106081 ttaaaacagt tgatgacgcg attcatttgt taaaagatcc gcgggcgaag gacatgcgct
   106141 tgatggtttt agtccctaat gtcaaagatg cggtaaaagt tgcgaaagct ttcccaacgg
   106201 aaatcgaatt aatgaacgtg gggaatgctg ggaaaatgac accgccatcg gaagataaga
   106261 aaaccttatc aaaggaagtg atgttaacga cgtcagaaat tcaagactta aaagaattag
   106321 ttgaacttta tccagatacc gtttttcagc ctacaccggc gatggaaaaa cgttcagcta
   106381 tctctatttt gagtgatttt tagaaaaagg aaggagaaaa attatgtatg atgcatttgt
   106441 agtcgctttg gccgttttta ttggaattgc tggacatgaa atgttcggga tgtccatgct
   106501 gagtcgtccg attgttgttg ctccgttagc aggagcgttg atgggggatt tgcagacggg
   106561 gttgattgtc ggggcgtcac tagaagccat ctttatgggt gtcgtcaata tcggaatctc
   106621 tagtacggcg gagccttctt tggctgctgg gctggctaca gcgttcgcaa tcaaaatggg
   106681 aggaaacgtg gatgcgatta ttcccattgt ctttccacta gcagtattag gattacagtt
   106741 aatgaatatg attttttctt ttgtatgtgg accgatggct gctaaatttt tcaaatttgc
   106801 aaaatctggt gatcaaaaag gaatcatcaa tctgcacttc tttatgtggt ttgtccacta
   106861 tgcgctttat gctcttattc cattctttgc cgttttattt ggagcagatg ttgtcaaaag
   106921 cgtcttggat aatattccag aggtcatcat gaacggatta acagtcgctg gaaatctgct
   106981 gccggcagtt gggatggcga tgctgttacg gatgctttgg agtaaaaata ttgctgtgtg
   107041 gtttttcttt ggggccgttg ttatggcgta tctcgagtta ccattgattg caattgccgc
   107101 agttggaacg attattgcaa ttgttatagc ccaaagagat ctacaactaa aaaatatatc
   107161 tgttcatgca gttcaaagcg atgctgctgt aaatgcaacg cttgaccaag aagaggagga
   107221 tttctttcaa tgaaattagc tgatggaatt tctaaagatg agaaaaaatt ggttcggaag
   107281 atgttttggc gctcgatgac tatgtatgct tcggtgaatc cggtgacgat gggcggcggt
   107341 ggtttcgcct attctatgat gcccttcatt aatcatttct acaaaagtga agaagatcga
   107401 gctgcggcgc tggaacgtca tacacagtat ttttcgacga ccattccttg cgcgtccttt
   107461 gtcatgggga ttgcggcatc gatggagaag gaaaattctg aaaaagctgg ttttgacgtg
   107521 gaaagtatca acgcgatcaa aacgagtttg atgggaccat tggctggaat tggcgattca
   107581 ttgttctggg gagtttggcg tgtcgttacc gcgggactag cgatcaactt agccaatgcc
   107641 ggaaatattt tggcaccaat tatcttttta gttatgttca atatacctaa ttacttgatg
   107701 cgatactatg gcggcgtttt aggctattca ttaggctcta aatatattga aaagctgtat
   107761 gcgaacgggt taattgaaat tctgacaaaa gctgctagta tcttgggtct gatcatggta
   107821 ggtgcaatga cgatcagtat ggtcaaattc aataccgttt tgagcttttc aatgaagggc
   107881 gaagaactaa tgaatgtcca agaaatcctc gatagtattt ttaaaggaat tgtaccgtta
   107941 ttggtaacat tgagctgttt caaattacta gataaaaaag tcggcgtcat caaaattatt
   108001 tttggaatgg tcgtcttggg aattttattg tcattacttg gaatcgcata gtaggaagct
   108061 gcagctacta ggctgcagtt tttttagcaa ggagggaaaa gatgactatt aatgaagtaa
   108121 tttattcacc caaacaccca ttttttaatg ctgagttaaa cgacggaaat gtgactactt
   108181 caaacgaata cagctactat cggaatattc ctgatgtaaa gggattgacc ttcgatgtaa
   108241 gtaatcaaaa gacattagca aaccttgatg tttgcgggac gattcaaacg ttgacctttt
   108301 atcgagaaaa tttactaact gaggaaaaac caggtgtttg ggtcaataaa cagctctctc
   108361 aaaccaattc gttatcctta aaggttgaag taaacgggat tgtctaccat ttgtcagaat
   108421 cggatcatcg tattgaagtc gatgtgattc aagactgttt gcctagatat attcatcatt
   108481 attcgacgtt taaggtcatt gtagtatcct ttgctcccat actggctaat aagcgtttaa
   108541 gtatgcttgt tcaacaggtt tacattgtga atgaaacaca cgaaagcctt caagtttcag
   108601 tgaaagatgt gccttcgtat caagagaagt attcggatca gcaaaatgtg ttgatttctc
   108661 aaaaaggaaa taaggacacg atcgcacaac aggaagtggc aagcttcgcc attgcttttg
   108721 tggacccaaa tgcttttgaa gaagtaatga cttttcagga atctgatatc gaaaaatggc
   108781 tgaaggaaac attgaactat tttgctcagc tatttgggca gctgtcaatg ccaaacgata
   108841 gagttgtcca tctctatcgt cgtgcacttt atcaaagctt ttctagtttc ggtatgaatc
   108901 gacagggaca ggtcgtcggc agcaattggg gcagctatcc tgctactaat cgaatttgga
   108961 ataaggatat gtattacgca tcattgccat tcaccatgtt tgaaccggag ttatgtcaaa
   109021 aaacggttct atggtttgat caatatggcg ttaagtttcc tggttcaaaa tttacaggag
   109081 gtatcaatca ttctctgagt aattcgttat ccagtgcgtt actggctagt ctttattttg
   109141 aacatactgg ggatacaaaa ttctttgagc ggtgtccgca ggttctactg aatgcttcaa
   109201 gaattattga ggacatatta gctcaacgtc ggtcagggga accaatgctt tttccatctg
   109261 tttggttatc agacgccttt gcacttggaa agtatcatac cggcagcaat ctttgtcttt
   109321 ggaaggcctg tattggactt gcagaaattt ttgaagccct agaacaactg agtcttagca
   109381 agcgatacaa aaacattgcc tgtaaactaa aggaatcaat ttgtaaacaa atgactatcg
   109441 atggtccttt tggtgagcaa tttttggagg gaatcggtga tgaggagaaa acaacctatt
   109501 cagtgaagca ctatcaaaaa ccgattcttg agcaagggtt aatcttttta agtgatgtga
   109561 ttgtggatgg aaaaattaat ttattgatgc atgatggaga agaatcagac actacgttga
   109621 tgccttttta tcaattttta gataataaag atcatttata tcagaacacc atggcctttt
   109681 ctgctagttc tttcaatcca acctatagcg aggaaatcaa aggcatcact tggggattag
   109741 aatccggcgc cacttttcct ggctttatca ctgttttaat gagcgagctg cacaaccctc
   109801 aagctttcgt aacccgatta gaagaactaa tttgtttggc agatttagac ggttcgtggt
   109861 ggtggtggcc gtatcgctta gaggctcaac aaggagaagt cgtgcgagac tttggctgcg
   109921 gcaaatgcgg ctgggcatcg ggtctttttg tcagcctaat gatcagtcaa tatttcggat
   109981 tgaaattcaa aaaaggagta ttgcaaattg atcctgttga taatttaacg ttttcttgga
   110041 agaatttgaa gttcggtcaa gccttcattt cgattgaatg tacgcaatcc gagttgagta
   110101 tttcaaattt gtcagaaaag ccattgaaaa taagtgatac gaaaaaaatt cttcacgtag
   110161 aaaagggaaa aacgattcac attcaaagga gaaaaagatg agtatactaa aaaatattac
   110221 ccaatttaaa aaaatccaga gtcgagcaat cactcccgaa aatccaacag gcgagaaagg
   110281 taaagctgcg atgttgccaa gcgagctagg accaagccgc aaaggtcgtg gcgcaattcc
   110341 gctgacttct ggtgaagaaa ctgtgattgc ggatattact ggaacgggaa taattcgtca
   110401 tatgtggatg acgattcgtg atcgaacaga atttggcagt tttgttttac gtgatttaat
   110461 cttaagaatt tattgggatg ggtcggatag ccctgctgtt gaaagtcctt taggggattt
   110521 cttttgtaat gggtttggag agcgttgcga ggtgaactcg ttgccgatag tggttaatcc
   110581 tacagggggc tttaattcct atttcgaaat gcctttccaa acatccgcta aaattacgat
   110641 cgagaattta catccgaagg atatgtcctc atttttttat acgataaatt acgctttggt
   110701 ggacgaattg gaagaaaata gcctatattt tcacgcaaat tggcatcggg aacgcattac
   110761 caaattacag gaggattata agttaattga tcgattaaag ggtcaaggct attacgtggg
   110821 tacttacctt gcgcttacct gcctagaacg ttattggtgg ggcgaaggcg agtttaaatt
   110881 ctacattgat ggggatagtg agtatccgac cgtaacctca acaggttcag aagattattt
   110941 tggcggggcg tgggcctttc atgagtacga tgagttcgga cgtccacatg cgaaaaacta
   111001 ttcaacagcc tttttagggt atccttacca atcaaataga gatgccacaa gagatcgttt
   111061 tgaaacggga aaattgaatg ccgttcatgc ctttggtgac gacgcaatgc cacgacatgg
   111121 tctttatcgt tggcatcttc aagaccccat ctgttttacc gaagatttat cggtcacttt
   111181 acaacaaatc ggcaacgatg atgttcgctt atttgagcgg caagacgatg ttgctagtgt
   111241 cgcttactgg tattcaacag aagcttcagg aaatactaaa cgtcatttca ctgatcgaga
   111301 acgcttgccg cgttaatgaa gtgtttttca catattcgaa gggaggaacc tatgatgata
   111361 tatggggcaa ttgaagctgg agggactaaa tttgtctgtg cagtcggtac tgaggaatta
   111421 acgattttgg aaagagtcag ttttcccaca acaactccag ttgaaacaat gaagcaggtc
   111481 attgattttt ttaaaccttt taaggaacaa ttggcaggca tcggggtcgg ctgctttggt
   111541 ccaattgata ttcaatgcaa atcaacgagt ttcggtcata tcacatcaac accaaaactt
   111601 gcttggcaaa atttcaattt tgtaggaacg ctaaagcaat atttcaatat tccgattgaa
   111661 tggacaacag atgtcaatgc ggcttgctat ggtgaatatg ttgctggttc tggcaaaggg
   111721 ttagcaggtg ttgcatactt cactgtggga acaggtatcg gaggtggtgc cttgttcaat
   111781 gggactttcg tggaagggtt cagtcatccg gaaatggggc atatgttgat caagcgacat
   111841 ccaaaggaca atttttcagg aaactgtccc ttccaccaag attgtttaga ggggatggca
   111901 gcaggaccag cgattgaaaa acggttgggt gtaaaaggcc agaatttatt ggctgacgat
   111961 tcgttttggc agattgaagc cttttacctt gcccaatgtg catacaatac aacactgatg
   112021 ttttcgccag atagaattat ttttggcggt ggtgtaatga aacaagaaca catgaagaaa
   112081 aaagttcagg ataaatttgt tgagcttata aatggatatg ttgaaatacc accaattgat
   112141 agctacatta ttacgcccga attaggtgat aatgccggaa taatcggcgg attagcattg
   112201 gccagaaaag cggtaagaaa taagcaacca taattaatga atgtttcatt gatactcatg
   112261 tcaaaagtat ttaaccgagt aatcaaatga tcaaatgaag ttggataaag cgaaaatgaa
   112321 ttcaaataaa aaggctctaa gtcaaatcgt gttgttttca ttaaatcact gaaaggaaat
   112381 gcttctgcgt ttcctttttt ctttttgttg tgcgctgtat accttgagga tattcactgg
   112441 tggcgtacac ttaaatagta atatattgtg ttactttatg tctgtggtaa tagttatagt
   112501 tgcttaccaa tgtatgttca tttttcgaaa tgttgacttc ataatccttg gcttaatgtt
   112561 tgaatttttt tggtgataga ttttttcagc cgtataaaat aaatcgtaat tctattatag
   112621 agagttgtga ttataaaaag atagcaagaa aaaaaaagaa agtttctata cattttaaat
   112681 attttattcc tatttctatt gctaaaaaag tagaaattct atatttacct ttaactctcg
   112741 cttctcttgc aaatgtagtt agcattttaa tcgataataa actcaggtag ttatattacc
   112801 ttgaaatgat tgataatttt gtcacttcta ttttttttgc aataaatttt ttattttagt
   112861 gaaaaataag aaactatttc tttgaaggcg ataagttaga aaaatattat tgtagtcttt
   112921 tctttattag gaatcatcac tgttgtatct tattaccttt tctgtttttc ttattagaga
   112981 aaaataattt gagaggtaga tagtttttat tggaggaagt aattatgtat atttttgaaa
   113041 caaaaatttt tcattgcaat aattgcaaca aagaaatttc atcgggggaa gaggtttgga
   113101 caaaaatgga aatttccgcc aaaagtaact gctcctcaac tgaaatcaat taaagaacta
   113161 gaatttgaga atgcactggt aatttgtatg acatgtactg atgaagtatt tcgaaaacat
   113221 ttttaaaggt tgagatgtat atagatttaa ccaatcattt atctctctcg gaaaaggaat
   113281 attaactaac cagcgggtta tcaaaaatag gtaggaaaac tgtacttaat ggcttatttc
   113341 gcgctgatta tgagaatatc agtctgtttg attaattttt agggttcaaa atacagagac
   113401 aaactccagg tcgggtttcg ccacaagcga attacccgat ctggagtttt gtctattgca
   113461 ggaaggcgta gccggactgc aggaataaag cccccggtt
//
LOCUS       DACDTH010000002        95903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDTH010000002 DACDTH010000000
VERSION     DACDTH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 95903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.002"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..560
                     /locus_tag="IWK54_000101"
     CDS             <1..560
                     /locus_tag="IWK54_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9592255.1"
                     /translation="KNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKH
                     IFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAH
                     KVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEV
                     GYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            760..1074
                     /locus_tag="IWK54_000102"
     CDS             760..1074
                     /locus_tag="IWK54_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9592256.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1095..1472
                     /locus_tag="IWK54_000103"
     CDS             1095..1472
                     /locus_tag="IWK54_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9592257.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1482..2255
                     /locus_tag="IWK54_000104"
     CDS             1482..2255
                     /locus_tag="IWK54_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592258.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2277..3680
                     /locus_tag="IWK54_000105"
     CDS             2277..3680
                     /locus_tag="IWK54_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9592259.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3862..5046
                     /locus_tag="IWK54_000106"
     CDS             3862..5046
                     /locus_tag="IWK54_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9592260.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5043..5333
                     /locus_tag="IWK54_000107"
     CDS             5043..5333
                     /locus_tag="IWK54_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592261.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5330..5551
                     /locus_tag="IWK54_000108"
     CDS             5330..5551
                     /locus_tag="IWK54_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592262.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5593..6372
                     /locus_tag="IWK54_000109"
     CDS             5593..6372
                     /locus_tag="IWK54_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP9592263.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6439..6933
                     /locus_tag="IWK54_000110"
     CDS             6439..6933
                     /locus_tag="IWK54_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9592264.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6989..7627
                     /locus_tag="IWK54_000111"
     CDS             6989..7627
                     /locus_tag="IWK54_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592265.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7699..8094
                     /locus_tag="IWK54_000112"
     CDS             7699..8094
                     /locus_tag="IWK54_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9592266.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8078..10531
                     /locus_tag="IWK54_000113"
     CDS             8078..10531
                     /locus_tag="IWK54_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9592267.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10528..12555
                     /locus_tag="IWK54_000114"
     CDS             10528..12555
                     /locus_tag="IWK54_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592268.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12552..13574
                     /locus_tag="IWK54_000115"
     CDS             12552..13574
                     /locus_tag="IWK54_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9592269.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13591..14520
                     /locus_tag="IWK54_000116"
     CDS             13591..14520
                     /locus_tag="IWK54_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9592270.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14765..14881
                     /locus_tag="IWK54_000117"
     CDS             14765..14881
                     /locus_tag="IWK54_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9592271.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14897..15700
                     /locus_tag="IWK54_000118"
     CDS             14897..15700
                     /locus_tag="IWK54_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9592272.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15875..16171
                     /locus_tag="IWK54_000119"
     CDS             15875..16171
                     /locus_tag="IWK54_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592273.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16178..18130
                     /locus_tag="IWK54_000120"
     CDS             16178..18130
                     /locus_tag="IWK54_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9592274.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18202..18699)
                     /gene="dfrG"
                     /locus_tag="IWK54_000121"
     CDS             complement(18202..18699)
                     /gene="dfrG"
                     /locus_tag="IWK54_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9592275.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            19061..20101
                     /locus_tag="IWK54_000122"
     CDS             19061..20101
                     /locus_tag="IWK54_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9592276.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            20199..20387
                     /locus_tag="IWK54_000123"
     CDS             20199..20387
                     /locus_tag="IWK54_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9592277.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20445..20798)
                     /locus_tag="IWK54_000124"
     CDS             complement(20445..20798)
                     /locus_tag="IWK54_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9592278.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21329..21751
                     /locus_tag="IWK54_000125"
     CDS             21329..21751
                     /locus_tag="IWK54_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9592279.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21748..21978
                     /locus_tag="IWK54_000126"
     CDS             21748..21978
                     /locus_tag="IWK54_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9592280.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22476..22676
                     /locus_tag="IWK54_000127"
     CDS             22476..22676
                     /locus_tag="IWK54_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9592281.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22703..23896
                     /locus_tag="IWK54_000128"
     CDS             22703..23896
                     /locus_tag="IWK54_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9592282.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23964..25529)
                     /gene="guaA"
                     /locus_tag="IWK54_000129"
     CDS             complement(23964..25529)
                     /gene="guaA"
                     /locus_tag="IWK54_000129"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9592283.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25588..26511)
                     /locus_tag="IWK54_000130"
     CDS             complement(25588..26511)
                     /locus_tag="IWK54_000130"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9592284.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26707..27312
                     /locus_tag="IWK54_000131"
     CDS             26707..27312
                     /locus_tag="IWK54_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9592285.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27620..28636
                     /locus_tag="IWK54_000132"
     CDS             27620..28636
                     /locus_tag="IWK54_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9592286.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28777..30078
                     /locus_tag="IWK54_000133"
     CDS             28777..30078
                     /locus_tag="IWK54_000133"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9592287.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30124..30786
                     /gene="deoC"
                     /locus_tag="IWK54_000134"
     CDS             30124..30786
                     /gene="deoC"
                     /locus_tag="IWK54_000134"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9592288.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30807..31205
                     /locus_tag="IWK54_000135"
     CDS             30807..31205
                     /locus_tag="IWK54_000135"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9592289.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31271..32353
                     /locus_tag="IWK54_000136"
     CDS             31271..32353
                     /locus_tag="IWK54_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9592290.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32565..34127
                     /locus_tag="IWK54_000137"
     CDS             32565..34127
                     /locus_tag="IWK54_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9592291.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34120..35253
                     /locus_tag="IWK54_000138"
     CDS             34120..35253
                     /locus_tag="IWK54_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9592292.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35253..36209
                     /locus_tag="IWK54_000139"
     CDS             35253..36209
                     /locus_tag="IWK54_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9592293.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36308..37474
                     /gene="deoB"
                     /locus_tag="IWK54_000140"
     CDS             36308..37474
                     /gene="deoB"
                     /locus_tag="IWK54_000140"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9592294.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37498..38316
                     /locus_tag="IWK54_000141"
     CDS             37498..38316
                     /locus_tag="IWK54_000141"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9592295.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38333..39040
                     /gene="deoD"
                     /locus_tag="IWK54_000142"
     CDS             38333..39040
                     /gene="deoD"
                     /locus_tag="IWK54_000142"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9592296.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(39338..40024)
                     /gene="gpmA"
                     /locus_tag="IWK54_000143"
     CDS             complement(39338..40024)
                     /gene="gpmA"
                     /locus_tag="IWK54_000143"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9592297.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(40196..40777)
                     /locus_tag="IWK54_000144"
     CDS             complement(40196..40777)
                     /locus_tag="IWK54_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9592298.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(40910..41593)
                     /gene="rpiA"
                     /locus_tag="IWK54_000145"
     CDS             complement(40910..41593)
                     /gene="rpiA"
                     /locus_tag="IWK54_000145"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9592299.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            41912..42325
                     /gene="rpsL"
                     /locus_tag="IWK54_000146"
     CDS             41912..42325
                     /gene="rpsL"
                     /locus_tag="IWK54_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9592300.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            42409..42879
                     /gene="rpsG"
                     /locus_tag="IWK54_000147"
     CDS             42409..42879
                     /gene="rpsG"
                     /locus_tag="IWK54_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9592301.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            42973..45060
                     /gene="fusA"
                     /locus_tag="IWK54_000148"
     CDS             42973..45060
                     /gene="fusA"
                     /locus_tag="IWK54_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9592302.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            45232..46419
                     /gene="tuf"
                     /locus_tag="IWK54_000149"
     CDS             45232..46419
                     /gene="tuf"
                     /locus_tag="IWK54_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9592303.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            46826..47134
                     /gene="rpsJ"
                     /locus_tag="IWK54_000150"
     CDS             46826..47134
                     /gene="rpsJ"
                     /locus_tag="IWK54_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9592304.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            47165..47794
                     /gene="rplC"
                     /locus_tag="IWK54_000151"
     CDS             47165..47794
                     /gene="rplC"
                     /locus_tag="IWK54_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9592305.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            47822..48445
                     /gene="rplD"
                     /locus_tag="IWK54_000152"
     CDS             47822..48445
                     /gene="rplD"
                     /locus_tag="IWK54_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9592306.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            48445..48735
                     /gene="rplW"
                     /locus_tag="IWK54_000153"
     CDS             48445..48735
                     /gene="rplW"
                     /locus_tag="IWK54_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9592307.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            48774..49607
                     /gene="rplB"
                     /locus_tag="IWK54_000154"
     CDS             48774..49607
                     /gene="rplB"
                     /locus_tag="IWK54_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9592308.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49650..49928
                     /gene="rpsS"
                     /locus_tag="IWK54_000155"
     CDS             49650..49928
                     /gene="rpsS"
                     /locus_tag="IWK54_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9592309.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            49951..50298
                     /gene="rplV"
                     /locus_tag="IWK54_000156"
     CDS             49951..50298
                     /gene="rplV"
                     /locus_tag="IWK54_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9592310.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            50312..50968
                     /gene="rpsC"
                     /locus_tag="IWK54_000157"
     CDS             50312..50968
                     /gene="rpsC"
                     /locus_tag="IWK54_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9592311.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            50971..51405
                     /gene="rplP"
                     /locus_tag="IWK54_000158"
     CDS             50971..51405
                     /gene="rplP"
                     /locus_tag="IWK54_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9592312.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            51395..51583
                     /gene="rpmC"
                     /locus_tag="IWK54_000159"
     CDS             51395..51583
                     /gene="rpmC"
                     /locus_tag="IWK54_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9592313.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51608..51874
                     /gene="rpsQ"
                     /locus_tag="IWK54_000160"
     CDS             51608..51874
                     /gene="rpsQ"
                     /locus_tag="IWK54_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9592314.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            51932..52300
                     /gene="rplN"
                     /locus_tag="IWK54_000161"
     CDS             51932..52300
                     /gene="rplN"
                     /locus_tag="IWK54_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9592315.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            52337..52645
                     /gene="rplX"
                     /locus_tag="IWK54_000162"
     CDS             52337..52645
                     /gene="rplX"
                     /locus_tag="IWK54_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9592316.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52673..53212
                     /gene="rplE"
                     /locus_tag="IWK54_000163"
     CDS             52673..53212
                     /gene="rplE"
                     /locus_tag="IWK54_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9592317.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            53231..53416
                     /locus_tag="IWK54_000164"
     CDS             53231..53416
                     /locus_tag="IWK54_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9592318.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            53452..53850
                     /gene="rpsH"
                     /locus_tag="IWK54_000165"
     CDS             53452..53850
                     /gene="rpsH"
                     /locus_tag="IWK54_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9592319.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            53882..54418
                     /gene="rplF"
                     /locus_tag="IWK54_000166"
     CDS             53882..54418
                     /gene="rplF"
                     /locus_tag="IWK54_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9592320.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            54579..54935
                     /gene="rplR"
                     /locus_tag="IWK54_000167"
     CDS             54579..54935
                     /gene="rplR"
                     /locus_tag="IWK54_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9592321.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            54956..55456
                     /gene="rpsE"
                     /locus_tag="IWK54_000168"
     CDS             54956..55456
                     /gene="rpsE"
                     /locus_tag="IWK54_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9592322.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            55471..55650
                     /gene="rpmD"
                     /locus_tag="IWK54_000169"
     CDS             55471..55650
                     /gene="rpmD"
                     /locus_tag="IWK54_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9592323.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55694..56134
                     /gene="rplO"
                     /locus_tag="IWK54_000170"
     CDS             55694..56134
                     /gene="rplO"
                     /locus_tag="IWK54_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9592324.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            56134..57429
                     /gene="secY"
                     /locus_tag="IWK54_000171"
     CDS             56134..57429
                     /gene="secY"
                     /locus_tag="IWK54_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9592325.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            57491..58138
                     /locus_tag="IWK54_000172"
     CDS             57491..58138
                     /locus_tag="IWK54_000172"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9592326.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            58329..58547
                     /gene="infA"
                     /locus_tag="IWK54_000173"
     CDS             58329..58547
                     /gene="infA"
                     /locus_tag="IWK54_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9592327.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            58580..58696
                     /gene="rpmJ"
                     /locus_tag="IWK54_000174"
     CDS             58580..58696
                     /gene="rpmJ"
                     /locus_tag="IWK54_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9592328.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58714..59079
                     /gene="rpsM"
                     /locus_tag="IWK54_000175"
     CDS             58714..59079
                     /gene="rpsM"
                     /locus_tag="IWK54_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9592329.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            59107..59496
                     /gene="rpsK"
                     /locus_tag="IWK54_000176"
     CDS             59107..59496
                     /gene="rpsK"
                     /locus_tag="IWK54_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9592330.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59576..60514
                     /locus_tag="IWK54_000177"
     CDS             59576..60514
                     /locus_tag="IWK54_000177"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9592331.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            60542..60922
                     /gene="rplQ"
                     /locus_tag="IWK54_000178"
     CDS             60542..60922
                     /gene="rplQ"
                     /locus_tag="IWK54_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9592332.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            61194..62033
                     /locus_tag="IWK54_000179"
     CDS             61194..62033
                     /locus_tag="IWK54_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9592333.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            62009..62878
                     /locus_tag="IWK54_000180"
     CDS             62009..62878
                     /locus_tag="IWK54_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9592334.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            62868..63665
                     /locus_tag="IWK54_000181"
     CDS             62868..63665
                     /locus_tag="IWK54_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9592335.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            63868..64614
                     /gene="truA"
                     /locus_tag="IWK54_000182"
     CDS             63868..64614
                     /gene="truA"
                     /locus_tag="IWK54_000182"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9592336.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(64947..67616)
                     /locus_tag="IWK54_000183"
     CDS             complement(64947..67616)
                     /locus_tag="IWK54_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9592337.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     NVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(67854..68042)
                     /locus_tag="IWK54_000184"
     CDS             complement(67854..68042)
                     /locus_tag="IWK54_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9592338.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            68312..68674
                     /locus_tag="IWK54_000185"
     CDS             68312..68674
                     /locus_tag="IWK54_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9592339.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            68726..70408
                     /locus_tag="IWK54_000186"
     CDS             68726..70408
                     /locus_tag="IWK54_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9592340.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            70525..72561
                     /gene="recG"
                     /locus_tag="IWK54_000187"
     CDS             70525..72561
                     /gene="recG"
                     /locus_tag="IWK54_000187"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9592341.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            72610..73611
                     /gene="plsX"
                     /locus_tag="IWK54_000188"
     CDS             72610..73611
                     /gene="plsX"
                     /locus_tag="IWK54_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9592342.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            73733..73975
                     /gene="acpP"
                     /locus_tag="IWK54_000189"
     CDS             73733..73975
                     /gene="acpP"
                     /locus_tag="IWK54_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9592343.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            74243..75247
                     /locus_tag="IWK54_000190"
     CDS             74243..75247
                     /locus_tag="IWK54_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9592344.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            75248..76192
                     /locus_tag="IWK54_000191"
     CDS             75248..76192
                     /locus_tag="IWK54_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9592345.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGIPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            76192..77154
                     /locus_tag="IWK54_000192"
     CDS             76192..77154
                     /locus_tag="IWK54_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9592346.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            77181..78095
                     /locus_tag="IWK54_000193"
     CDS             77181..78095
                     /locus_tag="IWK54_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9592347.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            78121..79902
                     /locus_tag="IWK54_000194"
     CDS             78121..79902
                     /locus_tag="IWK54_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9592348.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            80250..80936
                     /locus_tag="IWK54_000195"
     CDS             80250..80936
                     /locus_tag="IWK54_000195"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9592349.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            80956..84537
                     /gene="smc"
                     /locus_tag="IWK54_000196"
     CDS             80956..84537
                     /gene="smc"
                     /locus_tag="IWK54_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9592350.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            84546..85355
                     /locus_tag="IWK54_000197"
     CDS             84546..85355
                     /locus_tag="IWK54_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9592351.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            85366..86364
                     /gene="ftsY"
                     /locus_tag="IWK54_000198"
     CDS             85366..86364
                     /gene="ftsY"
                     /locus_tag="IWK54_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9592352.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            86551..88818
                     /gene="gshAB"
                     /locus_tag="IWK54_000199"
     CDS             86551..88818
                     /gene="gshAB"
                     /locus_tag="IWK54_000199"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9592353.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(88907..89359)
                     /locus_tag="IWK54_000200"
     CDS             complement(88907..89359)
                     /locus_tag="IWK54_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9592354.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            89509..90459
                     /locus_tag="IWK54_000201"
     CDS             89509..90459
                     /locus_tag="IWK54_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9592355.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            90452..91411
                     /locus_tag="IWK54_000202"
     CDS             90452..91411
                     /locus_tag="IWK54_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9592356.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            91408..92163
                     /locus_tag="IWK54_000203"
     CDS             91408..92163
                     /locus_tag="IWK54_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9592357.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            92186..93139
                     /locus_tag="IWK54_000204"
     CDS             92186..93139
                     /locus_tag="IWK54_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9592358.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(93783..94079)
                     /locus_tag="IWK54_000205"
     CDS             complement(93783..94079)
                     /locus_tag="IWK54_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP9592359.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            94434..94790
                     /locus_tag="IWK54_000206"
     CDS             94434..94790
                     /locus_tag="IWK54_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9592360.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            94800..95699
                     /locus_tag="IWK54_000207"
     CDS             94800..95699
                     /locus_tag="IWK54_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9592361.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        31582 a        17030 c        20162 g        27129 t
ORIGIN      
        1 taaaaaatga atttggaata gaagttaaag attattttat tgacttagat ttattacagc
       61 taataaacag tgtgaaaaga atgtatagtg gagacgacaa cggtgttgat ttatacaaat
      121 ttgttaccaa acatatattt gttaatctta atattgacga gaaaaattac ccgtctattt
      181 ttaagcacta tctatttttt attaacgaag tagaagatcg gataaaaaat aattcattat
      241 ctagtagtgt atctagtttt aagaagatgt ttaaatctcg agatggtatc acacttacta
      301 cagcacataa agttaaagga aatgagtatg atactgttat cgctattaat cttctaaacg
      361 ggaaaattcc tcattggaat gaaattttta atgtttctga taaaggaatt agttcagcga
      421 gaaaactact atacgtaatt tgttcaagag caaaaaataa tttatttctt ttctcagagg
      481 tgggttattt tacgagtaaa ggagacttgc tttatcccac tcaattatta gataaaatag
      541 aatttgatta taatatttaa aatacctagg tagtcgttgt tttaaatata gtaggaatcg
      601 gacgctcagt acggagcgtc tattttttta cccaattttg aaaggaagtg aacttatgaa
      661 aataaaaaat caagaatcaa aaggtcgctc tccccccttt aaaaccatca cacaaataag
      721 attcattcaa acataaaaac agaaaggata ggtaaaaata tggaacttaa atatgtcatt
      781 cccaatatgg aaaaaacatt tggacaatta gaatatgctg gtgaagggaa cattgaacaa
      841 cgtcgaataa atggcagacc aaccattctc tctcgtagtt acaatttata ctcaacgatt
      901 caacgagcag atgatattgt tgtcattctt ccctccgaag ctggcgagaa acattttgaa
      961 tccgacgaaa aagtcaaact catcaaccca catattaccg cagaaggtta taaaattggc
     1021 aatcgaggat ttaccaatta cattatgcac gcagacgata tggtgaaagc ataagaaagg
     1081 aaaggtgaaa aaatatgaga ttagcagaag gtattgtgat tgaaaaagac cagacatttg
     1141 gcacattgaa attttccgcc ttgcgtcgtg aggttcgtat ccagaatgaa gatggtacgg
     1201 tttccgaaga aattaaagaa cgtacctatg acttaaaatc aaaaggacaa ggacgcatga
     1261 tacaagtcag tatccccgct tctgttcctt tgaaagaatt tgactacaac gcagaagtgg
     1321 aattaatcaa tcctgtggca gataccgtag cgacggctac ttttcgaggg gcagacgttg
     1381 attggtacgt gaaagcagac gatcttgtat taaaaggaca gacaaaacct catcagaaat
     1441 ataaaaaaga agcgtccgaa agtgaaaaat aggtgttgct tatgacaaac acaccattga
     1501 ttttgaaaga aattccgaag gatgaagcca tttcttttat tcggcaatac cattattcaa
     1561 agatactccc tagattgtgt aaatactttc tagggatttt ttcagaagaa aaattattag
     1621 gcgttgtgga attaggctgg ggtacgcaac ccctacaaac catacgcaaa ctctttcccg
     1681 acagttccct tcaaaccaca gattatttag aaattggaaa gatgtgtttt cttccagaaa
     1741 tgaatcaaac caattatttt ggcagtcaag ctctttcagc tctaattaag tggttgaaag
     1801 aacatacgga ctgtcatttt ttatatacac tggcagatgg aattgaggga aaatgtggct
     1861 atgtctatca agcgtccaac ttcttttact gcgggtattt caagacaagt gtctatcgtg
     1921 ataagcagtc atgggaaaag atacaccctc gaagtgctcg ccttttatta gaggaaaatg
     1981 ctcgctttga acaggtagag aaaaagcatt ggctttccca ggcattttgc gaatacaaag
     2041 ggattgagaa aatcaatgga cgcatgtttc gctatctcta tccgttgaca aaagaagcga
     2101 aaaaactgtt aggacacact ctctatcgac gtcattatta tccgaaagaa aaaaatttac
     2161 gctttgaaaa gcgaatcgct tatcagaaat atgaagccat ttctcaacca acttttgata
     2221 aacaggctcg tatctacaat acacaattat tttgataggt ggaaaggagg atttttatgc
     2281 cattgcttca tgacagagga gagcgaatca aagccaacga taagcacttg gtctatcatt
     2341 ttgtactaat ttcattgctg gtagtttttc tagtcgtcct actccctttt cattggagag
     2401 cgttacaatc ggtgaactgg caagcagttc agttagatac atttttctat caacttcata
     2461 cgccatactt ttggagcagt attctcactg ctctttttgt atgcggactt atcagctggc
     2521 tttattatca ctatcgtata gattctataa aacagttaga gcaccgtcaa aagttagctc
     2581 gtatgatttt agaaaatcat tggtatgaag ccgaagaaac tcaatcagag ggattcttta
     2641 aagatttacc cagcaagaaa accaaacaac gaatccgcca ttttcctaaa atatattatc
     2701 gaatgaaaga tggactaatc catattcatg tagaaatcac cttagggaaa tatcaagatc
     2761 agcttttgca cctagaaaac aaactagaaa caggattgta ttgtgaatta gtggaaaaaa
     2821 cgttgaaaga ttcttatgta gagtatgtcc tactttatga tactattggc aatcgaattg
     2881 gcattgaaga cgtggtagct caagatggac gtgtcaaatt gatggaatcg gtctattggg
     2941 agttcgattc tctccctcac atgttaattg ctgggggcac tggtagtgga aaatcctatt
     3001 ttattctcac attgattgaa tctctcctgc atacaaatgc aaagctttat attcttgacc
     3061 ctaaaaatgc tgatttggca gacttagggg cggtcatgaa taatgtctat taccgtaaag
     3121 aagatatgtt ggcatgtatc aatcaatttt acgaagatat gattgatcgc agtgaaacca
     3181 tgaaacaaca ccctaactat aaaacaggag aaaattatgc ttatcttggt ttgtctgcca
     3241 attttcttat ttttgatgaa tatgtggcaa tgatggatat gctaggtcgt gaaagtacca
     3301 ccgttattca taaactcaaa caaattgtaa tgttaggacg acaagcaggc ttctttttaa
     3361 ttcttgcctg tcaacgtcca gacgcaaaat attttagtga tgggattcgg gacaacttta
     3421 acttccgtgt ggctttgggt cgtatgtctg aaatgggctt tggtatgatg ttcggaagcg
     3481 atacacaaaa acaatttttc ttaaaaccca tcaagggaag aggttacgtg gatacaggaa
     3541 aaagtgtgat tagtgagttt tatacacccc ttgtaccaaa aaggtacgat ttcttaggag
     3601 aaataggaaa agtcattcaa aaaaagcaat ctgagcctgt accgcacgaa gtgaaaggta
     3661 caggttcaga cttgctttga ttggtgtggc gttagccacg ccaatccttt agccccctct
     3721 atctaacagg ggggcacaaa tacaactgaa aacaggcaat tctcatcaac caatgttgat
     3781 ggtacaaagg attgcggaaa atatgaaagg tgtaaactca agggtcatag tttacatctt
     3841 ttttagattg gaggtttgcg aatggaaaac gaaaaaattt ggattaagga attgaaagaa
     3901 aagcgactgg cttatggcgt ttctcaaaat aaactggcgg ttgcttctca tattaccaga
     3961 ccttacctat cggatattga aacaggcaag gcagtcccta ctcaacaggt gaaagaagat
     4021 ttactgaacg ctttggaacg cttcaatccc gataacccat tggagatgtt attcgactat
     4081 gtacggattc gattccctac caatgatgtg gctcaaatta tcggtgaggt tttacgtttg
     4141 aatatggatt atatgctaca cgaagacttt ggctattatt cttatcccga acactatcgt
     4201 ttcggtgata ttgttgtcct agtctctcat gatgtaagta aaggtgtttt attagaactg
     4261 aaaggaaaag gctgtcggca atttgaaaac tttctattgg ctcaacatcg gagctggtat
     4321 gatttctttc aagattgcat ggaacataaa ggtattttca aacgcttgga tttagctatc
     4381 aacgataaaa cagggatttt gaatattcca gatttggcaa agaaatgtaa acaggaagaa
     4441 tgcatttccg tctttcgaag ctttaaaaat tatcgcagtg gtgaattggt tcaccgtgat
     4501 gaaaaaccat acatgggaaa cacactttat attggctcat taaaaagtga agtctatttc
     4561 tgtatatatg agaaagatta tgagcaatac gtgaaaaatg atgttccctt agaagacgca
     4621 gaagtaaaga atcggtttga aattcggtta aaaaatgacc gagccaccca agccatgaaa
     4681 gatttattgg cacatcaaca ggcggaaaaa acagcctttg aaattatcaa tcgctatatc
     4741 cgctttgcag ataaagacga cactaaacgt cggagtgatt ggaaaactaa tgaacgttgg
     4801 gaatggttta tcggtaaaaa tcgtggagca ttacgcctaa ccacacagcc agagccttat
     4861 tcttttgaac gaacattgaa ctggttacat catcaagtcg cccctacatt aaaaatcgca
     4921 agcattttag atgttctcaa cggtacaacc attatctcca ccatgattca ggaagccaaa
     4981 ctgacagaaa aacatgagaa attaattgaa caacaacatc tagccatgga ggatttgatt
     5041 acatgagcct tttaacaaaa gaattaaaga agttagggtt tcagtgtggc attgagtttc
     5101 aagcgtatat acaaaataca gggaaatata cgtctctcat tattgaggga aaacggcaag
     5161 ctggtgatac catctatacc tatgattttt acaaggtgag attttataat aattatacca
     5221 atcgagtaac cgtctatgga gagcatttaa ccccttttca attattaagg cgtgtaaaaa
     5281 gctatatcta ttatcgagaa aaatatctta aggaaaggag gacaatcaca tgaattttgg
     5341 gcagaatttg tacaactggt tcttggataa tgcacagtca ttggtattgt tagcgattgt
     5401 tgtcattgga ctgtttttag gttttaaacg agaattttca aaactcattg gcttcttgat
     5461 tattgctcta atcgccgtcg gacttgtgtt caatgcagct ggcgtgaaag atgtgttatt
     5521 aaacttattt aaccgaatca ttggtgctta acagatattt ttcatgataa aataggaagt
     5581 atatattttg ttatgaaagg atttatgaat atgtttacaa atgatcaatt taaatctctt
     5641 ttaattaaaa cattagaaag aaagagctct tcaaattatt atgaaggagg taacgagatt
     5701 gtttctaaaa tgaaaatatc agaggtaact ttagatgaga ctgacgattt tacttattat
     5761 aaaggatata aaaagggctg gaatacgctt tgcacatatc ttaaaataag agttcctttt
     5821 gatgatttag acttttttga aagtcataaa gatttgatta cccagacagc aagctccata
     5881 tatgataaac aaggtgataa tgtattagtg gatactattt tagtaccact tccagagaat
     5941 tacgaagtaa ttaactttag tcagttgaaa attagtgatg tggtttcaca agctattgaa
     6001 gacgctgagt cttttatgag taatggtgaa tatcaacgtg cctttgacag agttcacact
     6061 gcttttcatg gatatttaat tgaaatacta aaaaaatatg aaattacagt tcctagagac
     6121 gaaaatctat ctaaattata tagtaggata caacaattaa ttgaaaaaga aattcaacct
     6181 actgaacttg ctgatatagt gaagacaacg ataaggtcat caaatggaat gattagctct
     6241 cttaatgaag caagaaatag gcattcactt gcacacccta atacaaatat tattggaaag
     6301 agagaagcaa aactaataat aggtatttca agtacagtta cagattatat tagcggatac
     6361 cttgataagt agcgtcatta actgattgct acttttttta tgactatttt tataaaaagg
     6421 atgtgataag aattggaaat gaaagtctat gtggcaaatt taggacggta taatgaaggt
     6481 gaattagtcg gagcatggtt tactccacca attgatgagg aagaaatggc agaaagaatc
     6541 ggtttaaatg aggactacga agaatatgca atacatgatt ttgagcttcc ttttgatgtg
     6601 gacgaataca cgccgattag tgaaatcaac cgtctatgtg aagccattca agaaatcgaa
     6661 ggtacaccta tttataatga actcaaagaa attcaaggca tgtggtttag tagcttggaa
     6721 gaattactgg aaaacaaaga ggatattcat tgttatagtg attgtgattc gatggaagat
     6781 gtcgcccgtt attacgtgga agaaacagga cagcttggtg aagtcccttc caatttacaa
     6841 aattatattg actaccaatc tttgggacgt gatatggaaa tagagggtaa ttacctagtt
     6901 acctctcatg gggtatttga atattgtcag taagtttaac aatccgtgtt ataattaaat
     6961 taataaatag cacggagagg tataaattat ggtaaatgta tttgaaaatc ttaaactatt
     7021 aaaactagac atgaaagata aaggctgggt gatagattcc ttttactttc gttacaaaca
     7081 gcaaaattat attgtacttg ttaagctatt tgaaaaagag gaaaaagttc cagaatatgc
     7141 tctgttaaaa ctagaatttt taaaggaaaa cgatttttca gatatgctag ctgtctatgc
     7201 taattcggta aaattatata cagacacgaa aacaataaga gaatattttg gtatcgaata
     7261 cagtagtaac ttgggagatg ttttatttca atttagccaa actcttgctc gatttattcc
     7321 gacagaagtt agtgagaaga aaaatgaaga ccaaaaagaa gccatgtgtt tctcattaag
     7381 tcaaagtgat tcagaagatc ccagaaaaaa atattgtttt tcagttagaa gaaatccttt
     7441 aaagggaaat ggtgagctgg gaaaacgaag tccttttaat gacaataaaa cgagattgta
     7501 tagacccagc ttatatgaac gattagggtc agataccaat ttaagtttca gatattcaat
     7561 gaatccagat gatgaagaaa ctgatgaagg tattattgca aaatggacaa aaaataaaat
     7621 tgaatagtta aagcattgaa agagcaatca cctattttga ttgttctttt tctattttta
     7681 cggaaaggac gtgaagacat gaaaaaaata agaagttaca ccagtatttg gtcagtagaa
     7741 aaagtgattt atgcgattaa tgatttacaa ctccctttcc caattacctt tacgcaaatg
     7801 acatggttta tcgtttcttt gtttgtcgtc atactctttg gcagtttacc accactttct
     7861 ctgattgacg gagcactact caaatactta gggattccca ttgttctcac tgggttcatg
     7921 tgccaaaaga cctttgacgg aaagaaaccc tatggatttc taaagtctat cattacctat
     7981 gctctacgcc ctaaagtaac ctacgctggc aaacaaatac gattaaaaaa agaaacaatg
     8041 aatgaatcga ttacgattgt aaggagtgaa caactagatg gcatatccga ttaaatatat
     8101 ggaagataat ctcgtattta atcatgatgg cgaagtattc gcttattatg aattaattcc
     8161 ctataattac agttttttaa gttccgatga aaaaattcaa gttcatgata atttctgtca
     8221 attgattgcc caaaatcgtg atggcaagat tcatgctctg caactagcaa ccgaatcaag
     8281 tattcgagcc acacaggaac aatcgaaaaa agaaatcact ggacgattaa aagagatagc
     8341 ttacgaaaaa atagatgaac agaccgaagc gttaatttct atgattggtg acaatcaagt
     8401 agactatcgc ttttttattg gttttaaact ctacctcaat gaacaggaag tatccgtaaa
     8461 aaatgttggg aaagaaattc aaaaagcatt tgcggaattc tttcatggtg tgaatcacaa
     8521 attaatgggc gattttgttt ctatgcctat ggcggaaatc gaacgctttt ctaagatgga
     8581 acagttgtta gaaagtaaat tatccagacg ctttcagatt cgtaaattgg agaaagatga
     8641 ttttggttat attattgaac atctttacgg gcaaacaggt gtcccttttg atgactacga
     8701 ctaccattta ccgaaacgat acgaagataa aaaagtgttg gtaaagaaat acgatattct
     8761 caaacccact cgctgtttaa tcgaagaaaa tcaacgctat ttacggattg aacaggaaga
     8821 taccactact tatgtggcgt attttacaat taatagtatt gttggtgaat tggatttccc
     8881 taatagtgaa attttttatt accagcaaca gcagtttact ttccccgttg ataccagtat
     8941 gaatgttgaa attgtcgcca ataaaaaagc cttatctacc gttcgcaata aaaagaaaga
     9001 actgaaagat ttagatagcc acgcatggga aagcgacaac gaaaccagta ctaatgtggt
     9061 ggacgcttta gaatctgtcg atgaattaga aagtaattta gaccaaacca aagaatctat
     9121 gtacaaacta agctatgtgg tacgggtatc agcaaagaat ttggacgagc tgaaacaacg
     9181 ttgtgatgaa gtaaaagatt tttatgatga tctgtctatc aaattagtac gaccttttgg
     9241 cgatatgcta ggcttgcata atgaatttat tccctcaagc aagcgatata tcaatgacta
     9301 tattcaatat gtcacgtcgg attttttggc tggtcttggt ttcggtgcta cacaaatgat
     9361 tggtgaaaat tctggtattt acgtcggtta caatctcgat acaggcagaa atatttatct
     9421 ccaacctagc cttgcaagtc aaggaattaa aggctctgta acaaatgcat tagcgtccgc
     9481 ttttattggt tcattaggtg gcggaaaatc attctctaac aatatgctgg tctattattc
     9541 agtactcttt ggcggacagg caaccattgt tgacccaaaa gctgaacgtg gtgaatggaa
     9601 agaaacctta cctgatattg cccatgaaat caatattgtt aatctcacga gtgagaacga
     9661 caataaaggg ttacttgacc cttatgtgat tttaaaactc ccgaaagatt cagaaagcct
     9721 tgccattgat attctcacgt tcttaacagg tatttccagc cgagatagcg agaagtttcc
     9781 tgtcctgcgt aaagctattc ggagtgtaac tcaaagcaaa caacgagggt tgttattggt
     9841 gattgatgaa ttgcgaaaag aagatacgcc catcagcaat agtattgctg accatatcga
     9901 aagctttgtc gattatgatt ttgcccatct gttattcagt gatggcagtg tcacccaatc
     9961 cattagcttg gaaaaacaac tcaatattat tcaagtggca gatttggtct tacccgattc
    10021 caatagctct tttgaagaat acactaccat ggaattatta agtgtggcaa tgctgattgt
    10081 cattagtacc tttgctttag actttatcca ctctgaccgt tccattttta aaattgttga
    10141 ccttgatgag gcttggagtt ttttacaagt ggcacaagga aaagctctat ctatgcgatt
    10201 ggttcgagca ggacgtgcca tgaatgcagg ggtctatttc gttactcaaa acgcggacga
    10261 tttactagat gaaaagatga agaataatat tggcttgaaa tttgcctttc gttccactga
    10321 cctagtggaa atcaaaaaga ccttagaatt ttttggtgtg gacaaggaag atgaaaacaa
    10381 tcagaaacgc ttacgtgaat tagaaaatgg acaatgttta gtttcagaca tctatggtcg
    10441 tgtaggggtt atgcagtttc acccgatttt tgaagagttg cttcatgcct ttgataccag
    10501 accgcctgtg cgaaatgagg tggaagaatg acacaaaaac aaaagaaatt tcttcactat
    10561 agttggataa cgctcctttg tgtgggcgtt attttacttt tattaggcac gctgggcaat
    10621 gcagtacaag cgacaggctt agttgatgaa accattgata caagcaatga atactccaag
    10681 tatggtttga atcaatatca gcttgattat tatgtggaca atagctgggg ctggcttcca
    10741 tggaattgga gtgatggcat aggacaatcg gtgatgtatg gattgtatgc cattactaat
    10801 tttatttgga caatttctct ttatctatcg aacgccacag gttatttggt gaaagaagcc
    10861 tatgccttag atttcattag tcaaacatcc caagcaattg gcgaaaacat gcagacgatt
    10921 gcaggcatta caccgaatgg cttttccagt tcagggtttt atgtcggttt cctccttctt
    10981 tttattctca ttgttggaat ctatgttgcc tatacaggat tggttaagcg agaaacgacg
    11041 aaagtgatac gagctgtttt aaactttgtc gtggtgttta ttctatcggc ttcctttatt
    11101 gcgtatgcac ccgattatat tgcgaaaatc aatgattttt ccaaagatgt cagtaccgcc
    11161 agtctgaata ttgggacaaa aattgttctt cctaacacgg atagtcaagg gaaagacagt
    11221 gtggacatga tacgtgacag cttattctct gttcaagtaa agcaaccatg gctattgctt
    11281 caatatggct ccactgatgt agaagcgtta ggggaagaac gtgtggaaag tttattgtct
    11341 accagtccag atacaaacaa tggtgaagat agagaaaatg tagtgattga agaaattgaa
    11401 gatcatgaca atacctattt aaccctaccg aagacgattt ccagattagg aacagtcttc
    11461 tttttgtttg tattcaatat tggtatctcg atttttgttt tccttctaac aggaatcatg
    11521 attttttcgc aagtgctatt tattattttc gctatgtttt tgccaatcag tttcctatta
    11581 agtatgttgc cgagttttga tggcatgggc aggcatgcca ttaccaagtt atttaatgtg
    11641 attatgacaa gggctggtat taccttgatt attaccgttg cctttagtat ttccactatg
    11701 ctttattcgc tgtcagcaag ttctcccttt ttcatgatta tgtttttaca aattgtgacc
    11761 tttgcaggga tttattttaa actgggcgat ttaatgagcc tattcacttt acaaagcaac
    11821 gactctcaaa acatgggcag acaagtgttt cgcagacctc gtcaaatgat gaatcgacaa
    11881 accagaagat ttcaacgcag tatgagtaag atttttcgtg gaaaagcagg gtcaacgaag
    11941 caaaagacaa tgacaacaaa gcagaatcaa accaataaag ctaaccatac tcgtcaaaat
    12001 gaacggacta caccaaagaa agaaacagca ctttccaatg tgaaaaagca acaaggcggt
    12061 atcgggcaac gattaggtga aaagacagcc catgtattgg acacgaaaaa tcgtctagtc
    12121 aataaaagaa agcaggcaaa acaacagata caggcgacac caacccatgt caaatatgcc
    12181 cttcatcaag gaaaagagaa tgtcactcat aatgtaacag actttaaaca atctatggca
    12241 gataccaaag ctcaacgcaa acaacagcgt atgaaccagc aggagcaaaa acgaaagaac
    12301 attgctcaaa agcgtttgga gctggataaa gcaaaagaaa aaagacgaaa agaaaaccca
    12361 tcatctcctg ttcagaataa ccagcagcat gttcgcaagc gtcagcaaac cgtcacgcct
    12421 gttttgtcac aaaacactaa acgtgaaaat catcagcgac cattgaaaaa agagcaaaaa
    12481 caaaagacac caccgaagca attcaatcag aggaagctaa ataaaagaac ggataagcga
    12541 ggacaacaaa catgaagcta aaaataacag tgatttgtag tgtggtcttc tctctcttct
    12601 tttttctctt attattccta gtcattttct ttgcagatga tacggacagt ggcgaaaaca
    12661 acaaggattc ttctattcca caaggcggtg tgaccgtttc accagaagtg ttggcacacc
    12721 gtccgttgat tgaaaaatat ggtaaggaat atgggattga agactatgtt tcttatatct
    12781 tagccattat gcaagtggaa tcgggcggta cagcagaaga tgttatgcaa agttccgaat
    12841 ctttgggttt accgcccaac agtttgagta ccgaagagtc catcaaacaa ggggttaaat
    12901 atttcagtga attattaacc agtgcagaac aacaaggagt ggatatagat tctgttatcc
    12961 aatcttataa ttatggcggt ggctttttaa attatgtgag gagtcatggg aaaaaatata
    13021 cctatgaatt agccgaacag ttttctaaag agaagtcggg tggtcaaaaa gcagactatc
    13081 ccaatcctat agccatacct gtgaatggtg gttggcgata taactatgga aatcagtttt
    13141 atgttcagtt ggtttcacaa tacctaaccg acacttcccc aacagaattt gatgatgaaa
    13201 ccgtgcaagt cattatggac gaagccttga aatacgaagg gtttccctat gtctttggag
    13261 gggcgtcgcc taccacctct tttgattgta gtggcttaat acaatgggtg tatgacaaag
    13321 ctggcatttc tctccctcgt gtcgcccaag atcagtatga cgctacgcaa gaaatctcta
    13381 tggaagaagc ccaagcaggc gaccttatct tctttcattc aacctataat gcaggcacat
    13441 atgtgactca tgtggcgata tatttagagg gaaatcgctt ttatcatgca ggcgacccaa
    13501 tcggttatgg cgacttaagc agtcgttatt ggcaagacca tctgattggg gctagacgtg
    13561 ttattcataa ctaagaaagg aatgaatcaa atgattataa aaatcgagcg aaaagaaaag
    13621 aaggagaaac caaagaaatc taaaaaaccg agcaagcaaa gaaaagttcc gatgattaaa
    13681 gttggcacgc ataagaaatt aacctttgtt ttatgggttt tattgattgg tagtgtcgga
    13741 tttggaatct ataaaaattt tactgccatt gacactcata ctgtccgtga aacagaaatc
    13801 attaaacagc agattgtcga taccaaccaa gtagaaagtt ttgtgaaatc ctttgcgaaa
    13861 gactactttt cgtggcaaca atcccaagaa gccattgata aacgaaatga aaaattgacc
    13921 cattatttga cggaagaatt gcaggtatta aatgaagaaa tgattcgtaa ggatattcct
    13981 actagttcta gtgtgaacga tatacaggtg tggcaggttt ctcaagtgaa tgagaacacc
    14041 tttgaggtac tgttttctgt tgaacaagtt attaccgaag acaaagacaa ggaaactata
    14101 tcgtctagct ttcatgtggt tgtccatata gatgaatcag ataatatggt tattatcaaa
    14161 aatccgacaa tgagtaagaa acctcaaaaa tcggactacc aacccaaaca gctagaaagt
    14221 gaccatacag tagatacaga aacgatggac gaaatcacta gctttttaga aacgttcttc
    14281 cagctctatc ctaccgccac agaaaaagaa ttgacttact atgtaagtaa tcatgtgtta
    14341 cctatgatta acaaggaata tgttttcgag gaattggtga atcctatctt taccagaaaa
    14401 gataaccaag taatcgtgaa tgtcgctgtg aaatatttag atcaagaaac aaaggcaacc
    14461 caaatctcgc aatttgagtt gatattagaa aaacaagata attggaagat tgtaaaataa
    14521 caaatattgg tacatgatta cagatacttt gtaatcatgt actctttttg ataaaaaatt
    14581 ggagattcct ttacaaatat gctcttacgt gctattattt aagtgactat ttaaaaggag
    14641 ttaataaata tgcggcaagg tattcttaaa taaactgtca atttgatagc gggaacaaat
    14701 aattagatgt ccttttttag gagggcttag ttttttgtac ccagtttaag aataccttta
    14761 tcatgtgatt ctaaagtatc cagagaatat ctgtatgctt tgtataccta tggttatgca
    14821 taaaaatccc agtgataaaa gtatttatca ctgggatttt tatgcccttt tgggtttttg
    14881 aatggaggaa aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcagg
    14941 aaaaactacc ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag
    15001 cgtggacaaa ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac
    15061 aattcagaca ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac
    15121 gccaggacat atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc
    15181 aattctactg atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc
    15241 acttaggaaa atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat
    15301 tgatttatca acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa
    15361 acagaaggta gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg
    15421 ggatacggta atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt
    15481 agaagcattg gaactcgaac aagaggaaag cataagattt cagaattgtt ctctgttccc
    15541 tctttatcat ggaagtgcaa aaagtaatat agggattgat aaccttatag aagttattac
    15601 taataaattt tattcatcaa cacatcgagg tccgtctgaa ctttgcggaa atgttttcaa
    15661 aattgaatat acaaaaaaaa gacaacgtct tgcatattaa gtagttcagt tttggagtac
    15721 aaaaagtgaa aatgtaaacc taaatgagta tcaaattgca actgaatttg atactcgttt
    15781 tttattgtta aatcaacgtt tgttgttaat ttacaccaca aaatgtaagt taaaatgtat
    15841 ataaaaatgc gaacgtgaaa taattattgt aggaatgcaa attggtgtgt taaaatggtt
    15901 acaatttact aagcaaaaag gggtgcattt tcctatgcaa tttcttgagc caaaaaataa
    15961 gaacgctaag tctgtggatt gggaaatatc agagcaagta agggtgatag ttaagcaata
    16021 cgctgaatat gcagagcgta ctgaaagcga agcagtggat gaattcttat tgaacatttt
    16081 agacgataag aagtttattg aatggattgc caacaagcga agtaataagc ggattgttga
    16141 aaaaatgggt atcaaagata gagtgggttg atagacaatg accaagctta aaagattagc
    16201 cacgaaggaa gatgaaattg tagatgttaa aattccgatt tcaaacgaag aattaaaaga
    16261 tagggctaaa caatatgatt tattaactcc aaaacgattt gcaacaaggt ataacaaaat
    16321 gttgttcttg ccaacgtctt ttaaatggaa cggttctgaa tatcccattc aatataatta
    16381 ctgcatcaat ccgttttgtt gtaattttgg gaaagaacag cacaaattca aagatgtaaa
    16441 agggaaacca agtcgatata aaatgactgg tagttccaaa gataaaggtc ataaaggtat
    16501 gtactgcaat gacaatccta ttggcagagg tgtatcgcaa aactgtacag taaccccttt
    16561 atctaattgg tctgttgttg aagaaattaa acgccttata gaaatcaata gcatccaaga
    16621 tgttgagcca gattatcaat ttcataaaga aggctgctcg gaagaagaat caaccccatt
    16681 caatgagccg aagcaatttt acaaaagggg gaaaagtagg ggcaaatctc aacgctatca
    16741 atgtaaggcg tgtaagaagt tcactaatgt tctaccaaaa agagaagaaa ccactactta
    16801 tcaccaacaa aagaacacca ttcttccaat gttcgctaag atggttgttg gaagggtttc
    16861 agtcagtcgt acttgtgata ttttaggaat tggagttgga acctactatc ataagctgga
    16921 gtggctgtat aggcgttgtt tggagttttt agaacgatat gagacacaac cccttcaaac
    16981 aaagaaattt aacgagatgt ggcttaatac ggacaagatg cactactacc taaataacgt
    17041 ccgtaagaaa ggacaaggtt caaagaaata tacggggttt gaagacctta atatgcaaac
    17101 gtatatagtt gtatctgctg aggtcttatc aagatatgtg ttccgttctg atgttgccta
    17161 tgattggaat atttcaatgg atgaattaaa tgaagataca agaaaattca aagaagacca
    17221 tctcaatact tttagtcgta aaaatgatag gttagattgg tcttattatc cacaagaacc
    17281 atccgctaac gatagcgaaa ataggaatgc ttatcttcac gaattaggga aaattactaa
    17341 taggagtaga tttgtcgatg gtttaaatgt tgatgctcct tatactacaa ctgctcacta
    17401 ttggttaatt aaacaaatgg ttaatgccga cgaatggcga atgattagtg atgatgactt
    17461 ctctattaga aacgcctttt atagagtatt cacgaaagaa ttaaggttga gcgatgcaca
    17521 tcattttatt tgccaagtta acaagacgaa aagcagaaaa caatgcttaa aagaattcgg
    17581 acaagcaaaa gcagaactgc tcgattgggg agatatcaga ggattcaaaa caaagtttct
    17641 acggactttg gcatctcatt atctaactga attactaaca agtcatcaat tccacgaaga
    17701 ggctattagt aaagatggag aaagatatcg caaatatgcc gataacccta tcaaacatcc
    17761 attagccact aaggataaag gattttattc agttgattgt agaacggatt tatctgctct
    17821 ggaaccaaac gaaatcgcaa agatgctatt aaatgttaat gaccattcga ccaatagctt
    17881 tattcaacag ataagacgtt atatatcatc tcttgaaaga ccattaacaa cagctcgtgg
    17941 tgataagaag agctatattt acgctaactt caatccgaag tatgctcaat ttgcgattac
    18001 aattctaaga acttactaca acttttgtcg cccttttaaa tcagcggata aaaaggtttt
    18061 gacacctgct caaagattag ggattacaga taaacaattt gattggaaag atataattta
    18121 tttcaaatga caaaaagagc ttggagtaat cccaagctct tgtgctttat ctttactgtt
    18181 cttcttcaat taacgcaccc gttaactcaa taagtttttt ctttcatata catgaaaata
    18241 gtagttatac ggatttttat cattctttat ccctttttgg gcaaatacct cattccattc
    18301 ctcataattc acttctggaa aaaaagtatc tccttcgaat tcatgatgta tttttgtgat
    18361 gtacattttc tcaacataag ggaaaaacaa attataaatc tgttctcctc cgaaaataaa
    18421 aatttcttct tcgtttttac ataactcaaa aacatcttct attgaatgaa caatttcaca
    18481 accattaaag gtaaacccct tatctctcgt cagaataata tttcttctgt caggtaaggc
    18541 tcttccgatt gattcaaggt tcttcctacc taatattatc ggatgtccct ttgtagtatt
    18601 tttaacatat tcccagtcct tgggaatcct ccaaggaatg tcattctctt tgccaatcac
    18661 tctattctta tccatcgcag caatcaaaga aactttcatt tataacacag cctttttaac
    18721 taatttacca tctaatttct tatttctatc aaaaaacctt ttctcattat cctgcccgtt
    18781 agttgaataa gcaatttttt ctataagtta tattatcata ataccacaaa atcacaaatt
    18841 ttcattcaat tatcattttc aattgcattt tataaatcat tttgtatgta atttggtatg
    18901 taagttaaaa tgaaaaaagt agtccatttt gaagtacatt ttgtcatttt gtactccaaa
    18961 actgaactac ttacttgcat atatacgcct ttatagtgga gtactacatt tacgagattc
    19021 ggttagagta tcagaaaaag aaaaaataaa agttacagaa atgtatactt caataaatgg
    19081 tgaattatgt aagattgata gagcttattc tggagaaatt gttattttgc aaaatgagtt
    19141 tttgaagtta aatagtgttc ttggagatac aaaactattg ccacagagaa aaaagattga
    19201 aaatccgcac cctctactac aaacaactgt tgaaccgagt aaacctgaac agagagaaat
    19261 gttgcttgat gcccttttgg aaatctcaga tagtgatccg cttctacgat attacgtgga
    19321 ttctacgaca catgaaatta tactttcttt cttagggaaa gtacaaatgg aagtgattag
    19381 tgcactgttg caagaaaagt atcatgtgga gatagaacta aaagagccta cagtcattta
    19441 tatggagaga ccgttaaaaa atgcagaata taccattcac atcgaagtgc cgccaaatcc
    19501 tttctgggct tccattggtt tatctgtatc accgcttccg ttgggaagtg gaatgcagta
    19561 tgagagctcg gtttctcttg gatacttaaa tcaatcattt caaaatgcag ttatggaagg
    19621 gatacgctat ggttgcgaac aaggattata tggttggaat gtgacggatt gtaaaatctg
    19681 ttttaagtac ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac
    19741 tcctattgta ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct
    19801 tagttttaaa gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa
    19861 atattgtgca aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga
    19921 aattcctgct cgatgtattc aagattatcg caatgattta acttttttta caaatgggct
    19981 tagtgtttgt ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca
    20041 gacccgtcgt ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta
    20101 gtgcgtttta tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta
    20161 atatagattt gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat
    20221 tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa
    20281 atttcccttt atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga
    20341 atataataac aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa
    20401 actctcatct tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt
    20461 cttgcttcat tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag
    20521 ctatccatgt atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt
    20581 aagaaaaatt catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta
    20641 cttgggtgtt gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt
    20701 gttcccactt gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca
    20761 acggctctaa aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt
    20821 tactgttcct atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc
    20881 ctatagaaca ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct
    20941 gattttattt acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa
    21001 ttggcgactt aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct
    21061 taattaagtt cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg
    21121 tggttttgca gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt
    21181 ttatgtgctt ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac
    21241 aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc
    21301 ctttcacgac agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga
    21361 aaatcagttt gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa
    21421 acatctagac agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt
    21481 caactctttt tctactgttg atacgtacgt cactgatttc caactattta aattatttgg
    21541 attaagaatc aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa
    21601 aaaacggaac attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga
    21661 gttactaaat ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat
    21721 taaagaattc atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc
    21781 ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat
    21841 cgagggtata cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat
    21901 atggttattg atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta
    21961 tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt
    22021 cccgatttgg gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata
    22081 ggctaacaag ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa
    22141 gaaaagagct gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag
    22201 taattcgaaa gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata
    22261 ctcccgtata gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg
    22321 agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata
    22381 ttcttctctt gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg
    22441 agttttccta gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt
    22501 tagttgagct aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat
    22561 tgatgattac aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg
    22621 aagctgttga agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt
    22681 tgcacagtaa ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac
    22741 ctattatttt cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg
    22801 tagtggattc actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa
    22861 accagaagaa ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat
    22921 tttccttcca tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac
    22981 tattaagaag catttctctt attttgataa aatggctgtt tccgatattg agcctattga
    23041 tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt
    23101 tcaaggacta ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa
    23161 tccatctaag attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa
    23221 agaagaattt gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt
    23281 atttatctct ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat
    23341 tcaatgggaa gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta
    23401 caagaatcaa tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat
    23461 tgtattagat aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct
    23521 aattcagaca gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc
    23581 atatgctatt gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt
    23641 acggcattct catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga
    23701 tcgtctagga cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa
    23761 cagtaatttc gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca
    23821 aaattatgat acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat
    23881 gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa
    23941 gaggatttct agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg
    24001 atgtcataca cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt
    24061 tgcaagactt cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca
    24121 gcacggatac cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg
    24181 atccctggaa gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt
    24241 tcttcacgca agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg
    24301 ccaagaacac gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca
    24361 ggcatgccta actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt
    24421 tcgatcaatt caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc
    24481 gtctgtgctg tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg
    24541 aaagagacgc cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat
    24601 tcattcccaa tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag
    24661 aagcgatctc ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc
    24721 attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat
    24781 tggtcaccga tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa
    24841 agaccaagca agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg
    24901 ataaagttat ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc
    24961 agcaattcga ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa
    25021 ttacgtgttg tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca
    25081 aagccttcag gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt
    25141 ggtgtttctt taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat
    25201 tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca
    25261 tagcaaatcc ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca
    25321 ttctcatcat atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct
    25381 tttacttctt ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga
    25441 atacgtcttg tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca
    25501 acgttcgtca tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc
    25561 tcaaaatagt caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat
    25621 atgattttca gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc
    25681 ctgtaaattt ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc
    25741 acgatctccg atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag
    25801 taacgcacca aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata
    25861 gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt
    25921 attgatcccc tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat
    25981 gatatcatac acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac
    26041 acggttcaag aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc
    26101 catgattcca cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag
    26161 ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt
    26221 actttttcca acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta
    26281 tttatgaaga aataaacctt tactcaagaa caaagattca aattctttca tataaagata
    26341 aatcagatga gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt
    26401 caaacttttg atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa
    26461 ttctcgccat tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc
    26521 ctgcgttctc ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta
    26581 agagagattg ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct
    26641 tgacttgcgc acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg
    26701 gaagacatga caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa
    26761 tgggcttttg agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca
    26821 cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta
    26881 cctgatgaag gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg
    26941 gcatttgcga cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta
    27001 gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc
    27061 tatgaagcag ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct
    27121 ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt
    27181 acactgacaa ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa
    27241 gaaacatttg gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt
    27301 gtgaaagaat aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat
    27361 gtacgggtac gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt
    27421 ttcttcaaaa agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat
    27481 acagaacttg tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca
    27541 tactttcctt ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag
    27601 atagaaggga gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt
    27661 atcagtaaca actgtctcac agattttgaa taacaaagga agcagattta gtgataagac
    27721 aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc
    27781 aaatgtgatc aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt
    27841 cttcttctcc aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat
    27901 catgttatgc aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc
    27961 ccaccgctca gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc
    28021 attagacagc ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg
    28081 tcgggataac ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct
    28141 gtttattgaa aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca
    28201 attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa
    28261 tgggaaattt gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa
    28321 attactcaaa cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg
    28381 atgctatcag gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg
    28441 attcgatgga ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc
    28501 aagttttgat attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca
    28561 aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt
    28621 attgacaaag ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta
    28681 gtgctttttt tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa
    28741 tgtataatac cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa
    28801 aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat
    28861 acgaatggaa agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa
    28921 gacatgacaa atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc
    28981 atcgatttga gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt
    29041 gacacgacca cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa
    29101 atgtctggac gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc
    29161 ggatttcaga tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta
    29221 gcggtaattg gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt
    29281 gacgtcacag cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa
    29341 atcgcggcag gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg
    29401 aagaacatcg aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct
    29461 aaccgagaaa cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga
    29521 aatagtctag aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta
    29581 gtagaaatgt gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca
    29641 accgatgaag caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa
    29701 ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga
    29761 attttgactg cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa
    29821 atcgtggcaa atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa
    29881 gaagaagata ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg
    29941 acaaaaggtg agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt
    30001 gaattattat ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat
    30061 gacatcatca cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa
    30121 gaaatggaac taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa
    30181 gctgtgatgc aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat
    30241 ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca
    30301 gtaatcgggt ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat
    30361 gccatcaata acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca
    30421 aaggatgatc aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt
    30481 gcgcttgtca aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct
    30541 tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca
    30601 ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt
    30661 gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt
    30721 gctacaagaa tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc
    30781 tattaatttt ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag
    30841 ctattgaagc gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc
    30901 tcgtaacgga atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat
    30961 taacgaattg tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat
    31021 ttacgcatct agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc
    31081 gacaagtaat ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg
    31141 gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc
    31201 tataactcat gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg
    31261 ctttacagaa atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg
    31321 gttattttta ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc
    31381 atcagacaca acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt
    31441 caaccagtca gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg
    31501 aaatgatggc ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga
    31561 ccaagcattg aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc
    31621 gatccaagaa caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat
    31681 cgatggatta gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc
    31741 aggtgttgca gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa
    31801 aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa
    31861 agaattagga aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga
    31921 taaaggacgt tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc
    31981 ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga
    32041 caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt
    32101 aaacggcgaa aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt
    32161 agaagatttg gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta
    32221 tggattgaaa gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa
    32281 agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc
    32341 tgaagaaaac taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt
    32401 ttgattagaa caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga
    32461 acaactccct gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag
    32521 tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata
    32581 ttgtcataga aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca
    32641 tcaatttaca agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca
    32701 aatctacatt gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga
    32761 tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga
    32821 tggtccatca gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg
    32881 gaagcgagcc tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa
    32941 aagtttctga acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag
    33001 ttggaatgga acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga
    33061 tttttgatga gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca
    33121 tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa
    33181 tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca
    33241 acgttaaaga cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat
    33301 tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag
    33361 tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg
    33421 gcgaagtact tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt
    33481 tgactggttt gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta
    33541 ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata
    33601 aatacgggtt agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc
    33661 aaaaacctta cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag
    33721 aattgatcga agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat
    33781 caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt
    33841 tgattgttgc aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac
    33901 gtctgatcga acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag
    33961 aaattttaaa tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg
    34021 ttgatccgaa agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag
    34081 aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa
    34141 ttccgtaatt tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata
    34201 ttgatggctg cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc
    34261 cttggttcgc aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca
    34321 gcattaggtt tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa
    34381 gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa
    34441 gttgttttgc ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct
    34501 gttccaggtt tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg
    34561 aactatatct tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat
    34621 cgctcaagtt tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg
    34681 ttaacacaag cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg
    34741 cttattttcg tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt
    34801 ggattgaatc cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg
    34861 tcgatggtca tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg
    34921 tatcaaaact tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt
    34981 tccttgcttg gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt
    35041 ttgaagatcg gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat
    35101 gtatcgattg ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg
    35161 gctaaagtct tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca
    35221 acgaatcaaa accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga
    35281 tccttacttc tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt
    35341 tttctgaacg aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt
    35401 ttagttccat cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga
    35461 tcgctatttt agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa
    35521 cagtcagttt gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg
    35581 ctttaggtgt tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc
    35641 agaatctggg gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt
    35701 tctttaaaaa tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg
    35761 tgatcatctt caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag
    35821 ctgccgacac attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg
    35881 gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag
    35941 ctggtacgat cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga
    36001 atcctttagg ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca
    36061 ttggcgcaca attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat
    36121 atttgctgac aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa
    36181 acgggaagaa ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg
    36241 gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg
    36301 aataatcatg tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc
    36361 accagatgcc gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga
    36421 agcaggattg aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga
    36481 aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt
    36541 agggaaagat acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt
    36601 tcgtgtgttt cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg
    36661 ccgtaaagtc gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga
    36721 acaccaaatg aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat
    36781 cgcggcacat gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg
    36841 tgatataaca aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg
    36901 tgaaccagga gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg
    36961 acacacagtc cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat
    37021 caacgatatt ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga
    37081 tggcgttgac cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa
    37141 tttagtagat tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc
    37201 gattgaagaa tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt
    37261 gctaatgatt acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg
    37321 agaatatgta ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca
    37381 aggatattat tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga
    37441 aaatggcgaa agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg
    37501 accaagttaa gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa
    37561 gaaattgatt ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa
    37621 gatgctgtaa tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg
    37681 catgcaggaa agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg
    37741 agattccact actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg
    37801 gccggactag gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca
    37861 tttgtaccag gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct
    37921 ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat
    37981 acgcctgact atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa
    38041 gaaggcgtat atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg
    38101 tctcggacaa taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca
    38161 gctcacagcg gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg
    38221 caagcgaatc tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt
    38281 aaagcattgg ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt
    38341 gcatattgaa gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt
    38401 gcgagctaaa tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg
    38461 cggtatgtta ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg
    38521 aatgggaatg ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa
    38581 gaaattgatt cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt
    38641 agtactagca caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt
    38701 tcattttcct ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga
    38761 aaagggctat acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga
    38821 tttgacagag acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc
    38881 ggctctttat tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag
    38941 cgaccatttg atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga
    39001 aatgatcgaa gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc
    39061 cctgcattaa gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct
    39121 ggacttatta ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc
    39181 cgtttaactc tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata
    39241 ttgtgacttg tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt
    39301 gacaaatcgt gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa
    39361 ctgttaagtc atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca
    39421 tgatgtcttc gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat
    39481 gtgcagctac taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga
    39541 atggcaaagc gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt
    39601 ctaacattgc gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg
    39661 gaggaagagt atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag
    39721 ctgtttcttt tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg
    39781 atttgatttg tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga
    39841 ttgcacgagt taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt
    39901 tgcgtccgcc ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac
    39961 cagtaaattg gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg
    40021 gcatgtttag ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta
    40081 ttgtacaatt ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac
    40141 aagtagagaa tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga
    40201 tgcataattg ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg
    40261 aaacaccgca ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc
    40321 ggattttttt gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac
    40381 gagcttcatc taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta
    40441 ggttttccat taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa
    40501 gactaatcaa gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg
    40561 tagatttgcc taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag
    40621 cgatcactga ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat
    40681 ggaaaatttc ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt
    40741 catttatggt tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat
    40801 tttctaactg gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt
    40861 cgcaaatttt ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac
    40921 ggattgcgtc aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt
    40981 ttaggaaaag cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt
    41041 ctggctgttc gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta
    41101 agacttctcc gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat
    41161 gaagtagctg ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt
    41221 tatcgactaa tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta
    41281 ttttctcgaa aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt
    41341 catcagcacc gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta
    41401 ttcctaattt ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta
    41461 aaccttcttc acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg
    41521 tcccgagacc tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt
    41581 gttttaaatt catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta
    41641 ttttagcgtg atttacggta aaaagatacc attctttttt attccaataa agctttttca
    41701 aaaaaaattg catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct
    41761 ttatctacag gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg
    41821 atgcagacat tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta
    41881 gacgcaaatc gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa
    41941 acctcgtaaa tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag
    42001 ctttaagaaa actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt
    42061 gggtacaatg acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt
    42121 gtcaaactta attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca
    42181 cagtgttgta ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat
    42241 cgttcgcggt gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata
    42301 cggaactaaa aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact
    42361 atatagaagt aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa
    42421 ggtcctgttg caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact
    42481 cgcttgatca accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat
    42541 aatgcctttg acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa
    42601 gcaatgaaaa acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac
    42661 taccaagtac cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta
    42721 gtaaactacg ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc
    42781 atggatgctg caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg
    42841 gctgatgcca accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc
    42901 ttttataagt cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga
    42961 gaaacaagga gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg
    43021 gcccacatcg atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa
    43081 atccataaaa ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa
    43141 caagaacgtg gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga
    43201 atcaacatca tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg
    43261 cgtgtacttg atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact
    43321 gaaacagttt ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa
    43381 atggacaaga tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa
    43441 gcaaacgctc acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc
    43501 gaccttgtaa caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa
    43561 acagaaattc ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa
    43621 gctgtagctg aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact
    43681 gaagctgagt taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta
    43741 ctatgtggtt cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat
    43801 tacttgccat caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa
    43861 gaagtagaac gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg
    43921 actgacccat tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca
    43981 ggttcatacg tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag
    44041 atgcatgcca actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct
    44101 gttggtttga aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt
    44161 cttgaatcca tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa
    44221 gctgaccaag ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc
    44281 cgtgctgaaa caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac
    44341 ttagatatca tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg
    44401 cctcaagtat cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta
    44461 cgtcagtctg gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa
    44521 gaaggtgctg gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac
    44581 attccagctg ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat
    44641 ccattagtag atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat
    44701 gaaactgcct tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat
    44761 ccagtaatcc ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt
    44821 gatgttatgg gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct
    44881 ggcggccaac aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca
    44941 actcttcgtt ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa
    45001 gatgtaccaa aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa
    45061 tttcttaaaa ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata
    45121 gtttcgtgta aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat
    45181 acctatcaga caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa
    45241 gaaaaatttg accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat
    45301 ggtaaaacta cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa
    45361 gctatggctt acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc
    45421 tcaacagctc acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca
    45481 ggacacgcgg attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc
    45541 ttggtagttt ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct
    45601 cgtcaagttg gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac
    45661 gaagaattac tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc
    45721 cctggtgacg atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct
    45781 tcatacgaag aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca
    45841 gaacgtgaca acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga
    45901 cgtggtactg ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt
    45961 gaagttgttg gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc
    46021 cgtaaattgt tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt
    46081 gcacgtgaag acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat
    46141 acaaaattct ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca
    46201 ttcttcacta actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt
    46261 gaattaccag aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa
    46321 ttaatccacc caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt
    46381 acagttggtg ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa
    46441 agcaacattt attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc
    46501 ttttttattt attgcaggat caggaaaaaa gacaggagta ttataaatag aagaaacacg
    46561 aaataaaaga gtcagtattt tctaaatgaa actttatgaa aacaaaatgt tttttacaag
    46621 aaaaagcctt gctttgtgag tttatatata ttataatgac aaaggtgcgt ttactttagt
    46681 gaattgcaca gacgtaatca gaatgatttc gtcggctaag aaacgagagg ttgcgacacg
    46741 cccggacgct ttgccacgaa cgagcgtgac ggaaattttc gtggagctat gtctactttt
    46801 caaaataggc gaaggaggga acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc
    46861 gtatgaacac cgtattttag atcaatcagc ggataaaatc gtggaaactg caaaaagaac
    46921 tggagctagc gtgtctggtc caattccatt accaactgaa cgcagtcttt acacaatcat
    46981 ccgtgcgact cataaataca aagattcacg cgaacaattc gaaatgcgta cacacaaacg
    47041 tcttatcgac attgtgaacc caacaccaaa aactgttgat gctttgatga agctagactt
    47101 accatctggt gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata atggaggtgt
    47161 actcatgacc aaaggaatct tagggaaaaa agtgggaatg acacaaatct tcactgaatc
    47221 tggtgaatta attccagtaa ctgttgtaga agctactcca aacgtagtac tacaagtgaa
    47281 aacaatggaa acggatggct acgaagctat tcaagttggt taccaagata tgcgtgaagt
    47341 tttatcaaac aaacctgcga aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt
    47401 cattcgtgaa ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag aaattacagt
    47461 agatgttttc caagcaggag acattattga tgtaacaggt actacgaaag gtaaaggatt
    47521 ccaaggggtt atcaaacgcc acggacaaag ccgcggacca atggctcacg gttctcgtta
    47581 ccatcgtcgt cctgggtcaa tgggtccagt tgcacctaac cgtgtattta aaaacaaacg
    47641 tcttgctggc cgtatgggtg gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt
    47701 cgatgcagaa agaaatgtta tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt
    47761 aattactatc aaatcagctg tgaaagctaa ataattgtag gaagaaagga ggaactaagg
    47821 aatgccgaat gtagcattat tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa
    47881 cgaagaaatc ttcggaattg aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca
    47941 acgtgcgtca ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg ttcgcggcgg
    48001 cggacgtaaa ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc
    48061 accacaatgg cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt acagctacaa
    48121 acttcctaaa aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa aagttgctga
    48181 aaacaacttg gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa
    48241 acaagttctt gctaacttat ctatcgactc taaagtattg gttgttctag aaaaaggtaa
    48301 cgactttgct gctttatcag cacgtaactt accaaacgtt tctgtagtaa cttctgataa
    48361 tgtgagtgtg ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac
    48421 tcaaattgag gaggtgcttg cataatgaac ttactagacg taatcaaacg cccagtgatc
    48481 actgaaaaat caatgcttgc catggatgac aagaaataca cttttgaagt ggacactcgc
    48541 gcaaacaaaa ctttagtaaa acaagcggta gaagctgctt ttgacgttaa agtcaaaaac
    48601 gtgaacatcg tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc aggatataca
    48661 aaaaaacgtc gcaaagcgat cgttacattg actgaagatt caaaagaaat ccaattattc
    48721 gaagctgctg aataagcact aatagataac tcattaaatt aggagggaaa aacgtggcga
    48781 ttaaaaagta caaacctacc tcaaatggtc gtcgtaacat gacaggttct gattttgctg
    48841 aaatcacaac atcaacacct gaaaaatcat tgttacaacc attgaaaaac catgctggac
    48901 gtaacaacaa cggccgcatc actgtacgtc atcaaggtgg cggacacaaa cgccaatatc
    48961 gcgtgatcga cttcaaacgt aacaaagata acgttgttgc aactgttcaa acgatcgaat
    49021 atgatccaaa ccgttcagct aacatcgcgt tagtacacta tgaagatgga gtgaaagctt
    49081 acatcttagc acctaaagga ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag
    49141 atatcaaagt cggaaatgct cttccattag aaaacattcc agttggtact gttatccaca
    49201 acattgaaat gaaacctggt aaaggtggac aattgatccg ttctgctggt acaagtgctc
    49261 aagtacttgg taaagaaggc aaatacgtat tgatccgctt gaactctggt gaagttcgta
    49321 tgatcttagc tgcatgccgt gctacaatcg gttctgtagg taacgaacaa cacgaattaa
    49381 tcaacatcgg taaagctgga cgctctcgtt ggatgcgtaa acgcccaact gtacgtggta
    49441 gcgtaatgaa ccctaatgat cacccacacg gtggtggtga aggtaaagct cctatcggac
    49501 gtaaagcacc agtttctcct tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa
    49561 aagctaaatc agacaaactt attgttcgtc gtcgcaaaaa taaataatta ctttgaccat
    49621 gtgtcgcaca ttttgagagg aggttcacca tgggtcgtag tttaaagaaa ggaccttttg
    49681 tcgatgagca tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta
    49741 tcaaaacttg gtcacgccgt tcaacagttt tcccaagttt tgttggatat accatcgcag
    49801 tttatgatgg acgtaaacat gtaccagttt acatccaaga agacatggta ggacacaaat
    49861 taggtgaatt tgcacctaca agaacttatc gtggacatgc tgctgacgat aagaaaacga
    49921 aacgctaatt tgaggggagg ataaatccaa atggcagaac aaatcacatc agctaaagca
    49981 actgcaaaaa cagttcgcac ttcacctcga aaagcccgtt tagtaattga ccttatcaga
    50041 ggtaaaagtg tcgcagatgc aatttcaatc ttgaaattca caccaaacaa atctgctgga
    50101 atcattgaaa aagtattgat gtcagcagtt gcgaatgcag aaaataactt tgacttagat
    50161 gttgaaaact tggtagtaag cgaagctttt gttaacgaag gacctacaat gaaacgtttc
    50221 cgtcctcgtg caaaaggatc agcttctcca atcaacaaac gtacaagtca tatcacggta
    50281 gtcgtatcag aaaaataagg agggataatc agtgggtcaa aaagtacatc caattggaat
    50341 gcgtgtaggc atcatccgcg actgggatgc taaatggtat gctgaaaaag agtatgctga
    50401 gttcttacac gaagatttaa gaatccgtaa atttatttca actaaacttg ctgatgctgc
    50461 tgtatcaaca gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc
    50521 taaaccaggt atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt
    50581 aaacaaatta actggtaaaa gaatccatat caatatcgtg gaaatcaaaa aaccagatct
    50641 agatgcaaaa ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg
    50701 tcgcgctcaa aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac
    50761 tcaagtatct ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat actcagaagg
    50821 aactgttcct cttcatactt tacgtgcgga cattgattac gcatgggaag aagcagatac
    50881 aacatacgga aaactaggag ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa
    50941 aaaaaacact gagaaaggag ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt
    51001 cgtgaattcc gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt
    51061 gaatacggtt tgcaagctgt tgattcacat tggatcacaa accgccaaat cgaagctgct
    51121 cgtatcgcaa tgactcgtta catgaaacgt ggtgggaaag tatggattaa aattttccct
    51181 cacaaatctt atactgccaa agcaattggg gtacgtatgg gttctggtaa aggggcacct
    51241 gaaggatggg ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct
    51301 gaagaagtag ctcgtgaagc gttacgtcta gcttctcaca aattaccaat gaaaactaag
    51361 atcgtaaaac gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt
    51421 aaccactgcc gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt ttaatcttag
    51481 attccaatta gcaacaggtc aattagaaaa cactgcacgt attaaagaag tacgtaaatc
    51541 gattgcacgc atcaaaactg tgttgcgtga acaagctaag taattatgga aggaggccat
    51601 taagcgtatg actgaaggaa gaaatcaacg caaagtttat caaggtcgcg tggtttcaga
    51661 caaaatggat aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg
    51721 caaacgcatg aactattcta agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt
    51781 tggcgacatc gtgagaatta tggaaactcg tccattatca gctacaaaac gtttccgttt
    51841 gttggaagta gtcgaagaag cagttattat ctaataacac gagattttcc tatatagatt
    51901 gaatgcaaaa tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt
    51961 cgctgataac tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg
    52021 taaaactgct aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg
    52081 ggttgtcaaa aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg
    52141 tcgtacagac ggttcatata ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga
    52201 taaaagccct cgtggaacac gtatctttgg gccagttgca cgtgaactac gtgaaaacaa
    52261 cttcatgaag atcgtttcac tagcaccaga agttttataa tcttgagaca cttatcaaag
    52321 gaggtgcgaa acagttatgt ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa
    52381 agacaaaaac aaagaaggcg ttgtactagc agcgttcccg aaaaaagata aagttattgt
    52441 cgaaggtgta aatgtcgtta aaaaacacca aaaaccttct caagctgctc ctcaaggtgg
    52501 aattgttgag atggaagcgc caattcatgt ttctaacgtg atggtggttg actctactgg
    52561 tgtagctggc cgagttggct acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa
    52621 aaccggtgaa gttttagata aataaaagtt taaggaagga gggtcttact gaatgaaccg
    52681 cctaaaagaa aaatatctta aagaagtaac tccatcattg atggaaaaat ttaactatag
    52741 ctctgttatg caaacaccta aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc
    52801 ggtatcaaac gctaaaaact tagataaagc agttgaagaa ttaactttga tcactggtca
    52861 aaaaccaatg attacaaaag ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat
    52921 gccaattggt gcaaaagtta cattgcgcgg agaaagaatg tacgaattct tagacaaatt
    52981 ggtttctgtt tcactaccac gtgtacgtga cttccatggt gtaagtaaaa aagcctttga
    53041 cggtcgtgga aactatactt taggtattaa agaacaattg atcttcccag aagtagatta
    53101 tgatttagta gataaagtac gtggtatgga catcgttatt gtaacaacag caaactctga
    53161 tgaagaatct cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat aattaaagga
    53221 ggcgaactac gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg caaaattttc
    53281 aacacaagct tatactcgtt gcgaacgttg cggacgtcca cattcagttt atcgtaagtt
    53341 ccatctttgc cgtatttgct tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa
    53401 gaaagctagc tggtaaaaag taaattcgca taagaaggag gtaaaagatc aatggtcatg
    53461 acagatccaa ttgcagattt tctaactcgt atccgtaacg caaacatggt taaacatgag
    53521 tctttagaag tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa
    53581 ggtttcgttc gtgatgttga atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc
    53641 ctaaaatttg gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat ctctaaacca
    53701 ggtttgcgtg cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg tttaggtatc
    53761 gcaatcattt cgacttctga aggtgttgtc actgataaag aagctcgtga aaaaaatatc
    53821 ggcggcgaag ttatcgctta cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta
    53881 agtgagccgt atcggaaata aagtcgttgt tatccctgaa ggagtaacag ttactcaaga
    53941 cggaaacaac gttacagtta aaggaccaaa aggtgaattg actcgtactt tttcagcaga
    54001 tatcaaaatg aatatcgaag gaaacgaagt aactttcact cgtccaaacg acagtaaaga
    54061 aatgaaaaca attcatggaa caactcgtgc gaacttcaac aacatggttg taggtgtcag
    54121 cgaaggtttc caaaaagcgt tagaactaat cggggttggg taccgtgcac aattacaagg
    54181 atcaaaactt gtattgaacg ttggttactc acatccagta gaaatcacac caccagctgg
    54241 agtaactgtt gaagtaccag ctaacacaca agtaattgtt aaaggtgcga acaaagaaga
    54301 agttggcgaa ttagctgcaa acattcgtgg aactcgtcct ccagaacctt ataaaggtaa
    54361 aggtattcgt tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc
    54421 gtttagaaaa attgcataga agccagcagc aggaaaaagg catttggctg aagtttacct
    54481 tcaaaaaaat acatttttac ttgtgccgca gcttctattt ttcgaaacgt atttactgct
    54541 tgaaatagta gtacaataaa aataaagagg tgacaattgt gattacaaaa ccagataaga
    54601 acaaaacacg tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt actgctgagc
    54661 gcccacgctt gaacgttttt cgttctaaca aaaacatcta cgctcaaatt attgatgacg
    54721 tagcgggtgt aacgctagca agtgcctcta ccttggataa agaaatttca ggtggaacaa
    54781 aaacagaagc agcacaagct gtcggtaaac tagttgctga acgtgcagct gaaaaaggca
    54841 ttaaagtagt agtctttgac cgtggtggat acctttacca tggccgtgtg caagctttag
    54901 ctgaagctgc tcgcgaaaat ggactagaat tttagaagaa ggaggaatac cattcatggt
    54961 ttatatcgat ccaaaacatt tggaattaga agaccgcgtt gttgcaatta accgtgtaac
    55021 aaaagttgtt aaaggtggac gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa
    55081 aaacggacac gtagggtttg gtacaggtaa agctcaagaa gttcctgaag caatccgtaa
    55141 agcaattgaa gatgcgaaga aaaacttagt tgaagtacct atggttggtt caacaatccc
    55201 acacgaagta atcggtgctt ttagtggtgg tagaatccta atgaaacctg ctgtagaagg
    55261 ttctggggtt gctgctggtg gaccagttcg tgccgtatta gaattagctg gggtagcaga
    55321 tatcacttct aaatctttag gctcaaatac accaatcaac gttgttcgcg caacagttga
    55381 aggtttgaaa caattgaaac gtgcagaaga agttgcagaa cttcgcggca aatcagttga
    55441 agaattaatc ggataaggag gacaacaata atggctgaat taaaagttac tttaaaacgc
    55501 agtattatcg gacgtcctca aaaccaacgc gatacagtta aagcgttagg tcttacaaag
    55561 attaacagtt cagtggttaa gccttctaat gaagcaatta aaggcatgat caatactgtt
    55621 tctcacctag tggacgttga agaaatttaa tttagaaaaa tcataactag aacattaagg
    55681 aggtgccaaa cctatgaaac ttcatgaatt aaaacctgct gaaggttcac gccaagtacg
    55741 taaccgtgtt ggtcgtggta cttcatctgg taacggaaaa actgccggac gcggacaaaa
    55801 aggacaaaaa gctcgttcag gcggcggtgt gcgtttagga tttgaagggg gacaaacacc
    55861 attgttccgt cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag actatgcagt
    55921 cattaatctt gatgtattga accgctttga agacggagct gaagtaactc ctgttgcttt
    55981 agttgaagct ggaatcgtga aaaacgaaaa agctggaatc aaagtattag ctaatgggga
    56041 actaaacaag aaattaactg tgaaagcagc aaaattctct aaagcagctc aagaagcaat
    56101 tgaagcggct ggtggatcaa tcgaggtgat ctaatgttta gactattaaa agatgctttt
    56161 aaagtaaaag acattagatc gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc
    56221 ctaggtgcac atattactgt accaggcgta aatgctgctt cattgcaaaa cctttcttct
    56281 ttgccatttt tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa cttttccatt
    56341 ttctcaatgg gggtttcacc atacattact gcttctatcg tcattcagct tcttcaaatg
    56401 gacatcgtgc ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg taaaaaactg
    56461 aatcaagcga ctcgatatct gacattagta ttggcattta tccaatctgt cggtatcact
    56521 gctggattca atcaatacgc tcaattaggt ttcgtgaata atcctaacgt agcaacttac
    56581 gtaacgatcg gtttgattct aacagccggt acgatgtttg ttacttggct aggagaacag
    56641 atcacagaaa aaggtatcgg aaatggcgta tcaatgatca tttttgcagg tatcatttct
    56701 agattgcctg aaagtatcag agaaatttat gaagattact ttataaatat tgatccttca
    56761 gacatatgga aatctgctat ttttgtggcc attttactag tagcggtctt gttgatcgtg
    56821 acattcgtaa catttttcca acaagctgaa cgcaagatcc cgatccaata tacgaaacgc
    56881 gtagctggcg cgcctacaag cagctatctg ccgttgaaag taaatgcagc tggagttatt
    56941 ccagttatct ttgctagttc gtttattgct acgccaaatg ctgttttaca agcattgagc
    57001 ggtagattcg gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa ctataataca
    57061 gcacctggtg ctacgattta tacgattctg atcgttgcct tcactttctt ctacgccttt
    57121 gttcaagtaa accctgagaa attagcggaa aacttgcaaa agcaaggaag ttatatacca
    57181 agcgtacgcc ctggtaaggg gactgaagag tatgtctcaa gattactgat gcgtttaagt
    57241 acagttggtg cagtcttcct tggtttagtg gcattgcttc caatcattgc tcaaatggtt
    57301 tgggatcttc cacaatcaat cggattaggc ggtacaagct tactgatcgt tatcggtgta
    57361 gctttagaaa ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata tgttggattc
    57421 attgagtaag aaagcgtgtt gaggtttccc ctcaacacac gttctcacac atttattagg
    57481 agggaaaaca atgaacctca ttttaatggg gctgccaggc gcaggtaaag gaacacaagc
    57541 tgaaaaaatc attgatgctt atggtattcc gcacatctca acaggagata tgttccgcgc
    57601 agcgatgcaa aacgaaacag ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc
    57661 attagttcct gatgaagtaa caaatggaat cgtgaaagag cgactagctg aaccagatac
    57721 agagaaaggc ttcttattag atggctttcc acgtacgcta gatcaagcaa aagctctgga
    57781 cgcaatgcta aaagatttga ataaaaaaat tgatgctgtc atcgatatcc atgttggtga
    57841 agaaatcttg gtcgaacgtc tagccggccg atttatctgc agcaattgtg gagcaactta
    57901 ccataaaatt ttcaacccta caaaagttga agacacatgt gatcgttgcg gtgggcatga
    57961 attctatcaa agagaagatg ataaacctga gacggttaaa aatcgtctgg ctatcaacat
    58021 caaaaatagt gaaccaatct tggcttatta taaagaacaa ggtttgctaa acactattga
    58081 tggtgctcgt gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg aaaaatagca
    58141 cgattgcccg tagccgactt gcattatgca gttactcttg tcaagttcaa taaattatga
    58201 tagaatatgt cagtccgact tctagagaat cctctagatg atccattttg agaattttga
    58261 ggtgggaact gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg
    58321 tactgtgcgt ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc gaaactttgc
    58381 cgaatgcaat gtttaaagtt gaactagaaa acggacacca agttcttgct actgtttcag
    58441 gtaaaattcg tatgcattac atccgtatct tacctggtga caaagtaacg gttgaattgt
    58501 ctccatacga tcttaaccgt ggccgtatta cttatcgctt taaataattg tactccgtaa
    58561 aactacagga ggtattatca tgaaagtaag accatcagta aaaccaatgt gcgaacattg
    58621 taaagtaatt cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc caaaacataa
    58681 acaacgtcaa ggataattgg aggtgctata aatatggctc gtattgcagg agtagatatt
    58741 cctcgtgata aacgtgtagt agtttcactt acttatatct atggtattgg taacactact
    58801 gctaaaaaga tcttagctga tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg
    58861 aatgaacaaa cagatgctat ccgtgcagaa gtagataaat taaaagttga aggggatctt
    58921 cgtcgtgaag tgaacttgaa catcaaacgt ttgatggaaa tcggttctta ccgtggaatc
    58981 cgtcatcgcc gtggattgcc tgttcgtgga caaaacacga aaaacaatgc acgtactcgt
    59041 aaaggcccag ctcgcgctat cgctggcaag aaaaaataat tcttaagtga aggaggttaa
    59101 aacttcatgg cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca
    59161 ggtattgcac atatccattc tacattcaat aacactatca tcatgatcac tgatactcac
    59221 ggaaatgcat tagcgtggtc atcagctggt tcattaggtt tcaaaggaag caaaaaatca
    59281 actccttttg ccgctcaaat ggcagcagaa actgctacaa aagcagcaat ggaacacgga
    59341 ttaaagactg ttgaagtaac agttaaagga cctggttctg gacgtgaagc agcaattcgt
    59401 tcattacaag ctacaggttt agaagtgact gcaattcgtg acgtgactcc agttcctcat
    59461 aatggtgccc gccctccaaa acgccgtcgt gtttaatgag tgccgctcat tttgagtttt
    59521 atcagtaacg tcattgaaca agacactttt cgttttgaaa ggggtaaaga taagaatgat
    59581 tgagttcgaa aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat
    59641 cgttgaacct cttgaaagag ggtatgggac tactttaggg aattccctac gtcgtatttt
    59701 attatcttct ttgccaggtg ctgccatcac taatattcaa atcgatggtg tactgcatga
    59761 attctctacc atcaaaggtg ttagagaaga cgtcactcaa atcatcttga acattaaagg
    59821 cttagcgctg aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata ttaccggacc
    59881 tgctacagta actgctggcg atattatcgt tgatagtgat gtagaaattc ttaacaaaga
    59941 tatgtacatc tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc
    60001 tggacgtggt tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct
    60061 tccagtcgat tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg agaacacacg
    60121 tgttggacac cgtgaagatt ttgataaatt gacgatggag atttggacag atggttcaat
    60181 tgaaccgttg gaagcaatga gtttgtctgc gaaaatcatg actgaacatt tggatatttt
    60241 tgtgaactta actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca
    60301 aaaagaaaaa atgttggaaa tgacgatcga agagttagac ttgtctgttc gctcttacaa
    60361 ttgtttgaaa cgtgcaggaa tcaatactgt acaagaactt acaaataaat ctgagcctga
    60421 aatgatcaaa gtccgtaact taggacgtaa atcattagaa gaagttaaag caaaattgca
    60481 cgacttaggt ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa
    60541 cgtgagttat cgtaaactag gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt
    60601 aacaactgat ttattaatca acgaacgtat tgtgactact gaagctcgtg cgaaagaagt
    60661 tcgctcaact gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg
    60721 tcaagctgcg gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat
    60781 cgtcattgaa tcagctttac aaaaactgtt caacgatatt gcacctcgtt acgcagaacg
    60841 tcaaggcgga tacacacgca ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat
    60901 ggttgtctta gaacttgtct aaaatcacac taataacatc actttataga tgattctttt
    60961 agagtgttat gatgttggaa gatatttttc cgagtctagc tcagcgctgc tcctcaagcg
    61021 aatatcgctt ggggagtaag cactcatcat aaagtgtttt tttatacctt caattacctg
    61081 tcatgacaga tatgtttgaa ggcataaaaa aacattttaa gcgagaaata attgaaaatt
    61141 ttgtcttttt ttgatagtgt tagtaggaac tttaggatga agaaggagta atcatggagc
    61201 caattattga attagaaaag ataaattata aataccagcc agatgatctt cgccccgcat
    61261 tgaaagatgt ctcctttaca attgataaag gtgagtggat cgctattatt ggacacaatg
    61321 gttcaggaaa atcgacactt gccaaaacga tcaatggatt gcttctgcca gaatcaggga
    61381 tcgtgaaagt gggaaatcaa atattggatg aagaaaatat ttggacgatc cgacagatgg
    61441 ttggaatgat ttttcaaaat ccagacaatc agtttgttgg ttcgacagta gaagatgacg
    61501 ttgcttttgg tcttgaaaat caagggattc ccagagaaga aatgctagtg cgtgtgaaag
    61561 atgcacttga gaaagttaga atggctgagt ttgcttcacg cgagcctgct cgtttatcag
    61621 gagggcaaaa acaacgtgtt gccattgccg gcgtggttgc cttacgacct gatatcatta
    61681 ttttagatga agcaaccagt atgctagatc cagaaggtcg tgaagaagtg atctctacga
    61741 tcaagaagat caaagaagaa agtcaattga ccgttatatc tatcactcat gatattgatg
    61801 aagcagctaa tgccaatcgg atactagtga tgagacaagg cgaacttgtc cgtgaaggta
    61861 caccaaaaga aattttttct gctggtcctg aactgatcga cttaggtcta gatctaccat
    61921 ttcctgaaaa actgaagagc gcactgaaag aacgtggggt tgatgtgccc agtgaatata
    61981 tgacagaaga aaggatggtg gattggttat ggacatccgt tttgaacaag tagacttcac
    62041 ttatcagccg aatacaccat ttgaacaaag agccttattt gacatcaata tgacgatcaa
    62101 agaaaacagt tatactgcat tagtagggca taccggaagc gggaaatcaa ccttacttca
    62161 gcatttgaat gcgcttgtta agccaacaag cggtacagtc catattggag aacgagatat
    62221 tcagccggat acggataata agaatttaaa gcccatccga aaaaaagttg gcatcgtctt
    62281 tcagtttcca gaagcacagc tattcgaaga aacggtagca aaagacattg ctttcggtcc
    62341 taaaaacttt ggtgtcagcg aagaagaagc actagtccta gcaaaagaaa cattagaaca
    62401 agttgggctg gatgaaagct atttggaacg ttcgccattt gaactttcag gagggcaaat
    62461 gcgtagagta gcaatcgctg gtgtgcttgc catgaggccg gaagtacttg tattggatga
    62521 accaacggca ggacttgatc cccagggcag aaaagaaatg atggagatgt tttggcgtct
    62581 tcataaggaa cagcagatca ctattgtatt agttacgcat ttaatggatg atgtagccaa
    62641 ttttgcagat tatgtctatg tgttggaaaa gggacgaatc gttaatagcg gggagccgca
    62701 agaggtcttt caaaacattg aatggctaaa agaaaaacaa ctaggcgtac ctactgccac
    62761 cgagtttgca gaagaattaa tggctaaagg gatgaacttt gctaccttgc ctttgacagc
    62821 tgaagaatta gcagacgcta tcgtccatta tgctggagga aatgcccatg atgaataaat
    62881 tgatatttgg tcgttatatg cctggagatt ccttgatcca tcgattggat ccccgagcta
    62941 aattattagc cagtttttat tttatcggaa tcattttttt agcaaataat tgggaaacgt
    63001 accttttgtt ggcatttttc acattacttt ctgtcttctt gtcaaaagta gatttgaaat
    63061 tttttatccg aggcattcgc ccattgatct ggctaatcct cttcactgta gctttgcagg
    63121 tcttgtttac aagtggcgga gaaacttatt ggagttgggg tgttttccat atcactgaat
    63181 ttggtgtgat gaacggcctc tttattttct gtcgattcgt gttgatcatt tttatgtcga
    63241 ctctactcac gcttactaca ccgcctttgg aattatcgga tgcgattgag tacttgcttc
    63301 gtccattgaa agccgtacgg ttcccagtcc acgaaatttc attgatgctg tcgattgctc
    63361 ttcgttttgt gcctacattg atggacgaaa cagaaaagat catgaacgct cagcgcgctc
    63421 gcggtgttga ttttggtgaa ggaaacctag ttcagaaaat gaaagcagtc attcctttgc
    63481 tgattccttt atttgtcagc agcttcaacc gtgcggaaga tcttgcgact gctatggaag
    63541 caagaggcta caaaggtggg gaaggaagaa cgaaatatcg tgtattgcat tggcataata
    63601 gagacacgat ggttatcatt gcttttgtat tattaactgt gctactgatc tttttaagag
    63661 gataaacgtt cagtgggccg ctatctattc ttttcttgct gatctgtttg caagaaagga
    63721 tgcaagcagt gtatgacaag aaatattagg gtctgtggca gactaaggtc acaggcctct
    63781 ttcttttatc tgaaaagaaa gaacgaaaaa attttataga aaaatcatta ttccagataa
    63841 ccttttagga acaaaggaga gaaaaacatg gtacgttaca aagcaatcat tgcgtacgac
    63901 ggaacaaatt ttaatggatt ccaaaaacag ccaaatgggc gtactgttca agaagaagta
    63961 gaaaaaacat tacaaaaaat ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg
    64021 acagatgccg gagtacatgc gatcgggcaa gtaatccatt ttgattatcc agaggagcgt
    64081 ccgttagaac gaatgcgttt cgcgctagat acccagtcac cagaggatat tgctgtaaga
    64141 caagtagaaa ttgtttcaga agacttccac gcaagatatt tggtcaaaga aaagacgtat
    64201 caatttcgag tggatatagg gaaaccacgc agtccttttc gacgccatta tgcaagttat
    64261 tttccttacc cacttgattt ggaaaaaatt cagcgggcgc ttcctgattt attagggact
    64321 cacgatttca cctctttttg tgcatcagga agctcgatag aggataaaat acgtacgatc
    64381 tacgaggcta agatggaggt aaatgaaaca aaagacgaat tgttgtttac atttagggga
    64441 aatggcttct tgtataagat gatacggatc ttagtaggaa cgcttttgaa aattggcaat
    64501 ggccgattgc ctgaagatag tatcccagat attattgcga aaaaagatcg taatgcagca
    64561 ggacctacag cacaccctga aggactttat ttgtatgaag tgaaatatga ataagaggtt
    64621 atgatagaag tggtcagctt caagaaataa gaaggtattt acgaaaatta cttctcaaaa
    64681 ttttgcaaaa tttcggctta ttttcgaaga agctacttct ggaacaccgt ttaagaaaga
    64741 ggtcgtgagc ctgtcattta actctgagtc acaaagagca atttcaaaaa acagcttctc
    64801 atctttgatg aatatcgtga cttgtggacg aggcgttgat aagtgaatca tctttttgtt
    64861 acctattttt tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc ttggtgcgga
    64921 cagtctgaac aggttccatc gtttctttat tttctacgtc ttcttctgtg tgtgtttcgt
    64981 ggttgttctt ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg gtcaaggtac
    65041 cagacattaa gaataaacaa tgcaatacat aggacaaaaa ggattgcacc tatcgtaaat
    65101 ccttcaggca agtaactgaa agtgacagta tgtttacctg ctggaacttt tatcatcaag
    65161 aatgcgtctt tgaaattgct gaccttgact ggtttcccat caattttagc tttccatcct
    65221 ttgtcataag gaatagttgt caccaacatg gaatcttcag cggtatttac agtcccagaa
    65281 gcttttctgc cagtggcttc catttcgaca cctttttctt tgatctgatt cattgcggct
    65341 tgataggcta caacatctag accaacgact ttaggctcca tgaaacttat ttctttcgtc
    65401 ccataaaaac ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc aagatcatag
    65461 tattgaccag aaatgttgat ttgcgttttt cgcgatgtac cattgacagt gatcgtcgca
    65521 ctagaacttt ctaattgcgc aaaattggtt gggaacaaac ttaagtaagc ttgtgtattt
    65581 gcaggcacat ggacttccca agtgagctct tttggctggt tatctgctgt ttctgagtaa
    65641 gtgacgccag tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa agtaggtgta
    65701 tagaatgtaa aatactgttc atttagacca gacaatgtat tgatcaagtt tgtctgactg
    65761 gtcaaattat ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc agtaaatgct
    65821 agtggcaatg cattgacatt ctgatacagt gtatagtcgc cgctttgacc agcttggcgg
    65881 aagccatatt tactgaacgt gctttcgttt ttagaaatgt tatatttgat tcctgtaaac
    65941 ccatccatca gcaaagtatt attgttataa cggatattca aatttgtccc tcttgaacgg
    66001 aaacctaatt tatccaggta agaggaagaa ttacgatttc ttatagaaga aaacaaactg
    66061 ataccactgt aaccatagtt gatcgaatcg tttgcagata ctggatccag attttccaag
    66121 cggtaaaacg agtcggaatc tttttttgtt tcatttacaa gagctttcaa gtcaggataa
    66181 ggatcagtat ataaactgcg ggaagcataa ttccaatcat caagaatgcc atggatcata
    66241 ctgttcgtat tgaccagtgc ttcagcagac atgatcaata ataacaatat actcagatga
    66301 ttgaatgaaa gttttttatg ctgataaaat cccacagcaa aagcataaat ccctaagaaa
    66361 accactgtca atacaaagga agtcatcgtc acataggtgt agcttccttc aggtttgatg
    66421 ccccatgcca ccgcaaagag agcagctacg accaaaatga ttcctaacaa cagcccacca
    66481 tcgtcttgtt ctatcttttc ccatcctttt cctgcaagca ggagcactaa gaaagagaac
    66541 agataggcat aacggaataa aaacatattc ggtgcatgca taccatgcca gaacaaattc
    66601 aatggaacaa gataaaaact tgcgataagt atggcgaaaa gactgccgta caaaaattta
    66661 tttttcaacg gtactttccg actggcaaaa taaaacagac agaggactaa tggtagcaga
    66721 cctgcgtaaa cgaacggaat cgatccgtat tttgtcgtgt cataaacacc aatcatattt
    66781 ttcatgatta gatccagata agaagtcgct tctgttttca acgtagtgat ctgactcagt
    66841 gcctcgccat ttgaccgaag atccagcact gctggcaata caatgatcat tgaagcgccg
    66901 cctgccagta aagaagtgat accataagga acaatcgttt ttttatattc tgaccagttt
    66961 gtcaatagac gggcgataaa atataagaaa gaaaatacgc caatcataaa acccatataa
    67021 aaactggata aaaacaagag taaataactg ataaataaaa gtttcggccg tttaagatcc
    67081 ataacacgat gaatccccaa aatcactaat ggaagataaa caaatgcatc cagccacatg
    67141 atgatctccg aatgtgcagt gataaatgag atcaatgcgt aactgacgct aagtgcaaca
    67201 tgcccccagc gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa tccggcacag
    67261 ccgaccttaa gcaaagtcag aacatacagt gcatctggca tcatctggtt tggaaaaaag
    67321 aacaccagtg gcgtgaagat cccgcctaga tagtaagaaa ttaaagataa ataattcaat
    67381 cctagagaag cattccatgt ataaaaaata ctttgctttc caagaagaac atttcggaag
    67441 ctggcatgaa aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact tctgctgctt
    67501 ccaggataga ttccgatact tagataaaca agtgctaaaa tcaaaaacgg aatcaggaaa
    67561 ctagctgctg cgtagcgtcc gttttctcta aagaatgact ttaaattttt tttcatattt
    67621 attttccacc aaatttctat gaagttgtat tcagtttatc atatccttgt ttttctctgc
    67681 ttaaaaagag tctagttttc acaaaaaaat aaaatttaga ataaataact tcataatttg
    67741 ttcgtataac aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca cccttaaaaa
    67801 tttttattaa ataaaaaacc gttcactatc tgaacggtcc aagtatcatt caattaaacg
    67861 cgttcaactt ttcctgattt caaagcacga gttgacaccc aaactttttt aggttttccg
    67921 tcgaccataa cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt tgagttcata
    67981 gcgtgtgaac ggttgtttcc actttttgtc ttgcgacctg taaagtaaca aacttttgcc
    68041 atggtaattt cctcctttgc cttgatttgg agcaatagaa taattcagct cataccagac
    68101 taacatatca tatagaatgg gaatttgcaa gtacattttc atagatacaa ttgacaattg
    68161 agaagaataa ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt ttttgagtac
    68221 tttgggtaac tgccttttat ttcaagttgc gctatgataa aatatgatag aagaaaaatg
    68281 ggagtttttt acccgttaag gaggctcatt catggctgta aaaattcaaa cgtcagctgg
    68341 aaccattgaa attaccaatg atgtaattgc aacagttgtt ggtggcgccg cgacagatgt
    68401 gttcggtgtt gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta ctgagatttt
    68461 gcgtaaagaa aattactcaa gaggagtagt tgtccgccaa gaagataatg gtattgctgt
    68521 agatgtttat acgattatca gctatggtac gaaaatctct gaagtttccc gcaacgtcca
    68581 agaaaaagtg aaatacaatc tagaggcatt attaggcgta actgccaatt cagttaatgt
    68641 ctttgtacaa ggtgttcgtg tactgccaga ctaggcagga gactggctgg taactcagca
    68701 gtcgatctga aggaggatta tctaggtgag tgtaacagaa ataagtgcaa gccagttcca
    68761 ggaaatggtc caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg tcaattcatt
    68821 aaatgtattc cctgtgccag atggcgatac gggaacaaat atgaatctat ctatgactag
    68881 tggagctaag gcagtagtag attcaacctc agaaaaagta ggagaattag cagcaatgct
    68941 ttcaaaagga ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt ctcaattgtt
    69001 tcgtggattt tctaagcaaa ttccagatgt tgtctcatta aatgcttctg atcttgctgc
    69061 agccttcact catggagttg aaactgcgta taaagcagta atgaaaccag tagaaggaac
    69121 gattttgaca gtagctagag aagcagcgaa agctggagaa aagaaagcaa aaagtactga
    69181 tgatgtcgtt gaagtaatga cagctgttgt aaaaggagca aagcgtgctt tagctaaaac
    69241 tccagacttg cttccagtcc taaaagaagt cggtgtcgta gacagtggag ggcaaggact
    69301 gctgtttgtt tatgagggct ttttaagtgc attgaatgga gaattccaag cggatgaaac
    69361 atatgaacct tctccagcag aaatggatga tatggtcaat gcagaacatc atcgcagtgt
    69421 ccaaggtcaa atggcgacag aagatatcca ttttggttac tgtacagaaa tcatggtaaa
    69481 aatcggagaa ggacctacag tcgatagtac attcgattac gaagaattcc gtaactattt
    69541 agatggtata ggagattctt tacttgtggt caatgatgac gagatcatca aagtccacgt
    69601 gcatacagaa aacccaggag aagtcttgaa ttatggacaa aaattcggtt cattagtcaa
    69661 aatcaaagtc gacaacatgc gcttgcagca tgagacgatt ttggaacacg atgaagaagt
    69721 gaacgagttt gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag cgatcgctgc
    69781 tggtaaaggc gtccaagagc tgtttgaaag tttaggcgct aattatgtta tcagtggtgg
    69841 acaaacaatg aatccaagta cggaagacat tgttaaagcg attcaagaag taaacgcaga
    69901 taaagtgatt gtcttgccaa acaataagaa catcttcatg gcagctgatc aagcagcaga
    69961 agtagcagat atcccagtag cagttgttcc ttcaagaacg atttcccaag gattgacagc
    70021 tatgctttca ttcaatgatc aagcttcttt agaagaaaac aaagaaacga tgacagaaat
    70081 gttgtctagc gttgtgagcg gtcaagtcac tcacgcgatc agagatacct caattgacgg
    70141 tgtagagatc aaagaaggag actttttggg catgatcgat ggaaaaatcc tgatctctaa
    70201 cccagatatc acagcaacag cgttaagtac actagaacaa atgatcaatg aggacacaga
    70261 gatcgtaacg atcttggcag gtgatgaagg aacagttgaa caagcaaatg aatttgctga
    70321 gaaactaaca gaaaaacatc ctgatttgga aatcgagatc caccaaggag atcagcctgt
    70381 ctatccttat ctattttctg ctgaataatt tctgtgtcag aatatctttc taacacagta
    70441 ttcattcata aaaggccttc ggttagtgat caggtatgat cgctgaccaa gtcctttttt
    70501 tgttaaagga gggaggaaaa atttatgcgt tctttagatg atccaatcac tatgctggca
    70561 ggagtcggag aaaaaagagc agctagtctt gccagcttgg gcatccaaac gattgaagac
    70621 ttactgactt attatccttt tcgctacgaa gatattcagg aaagaaatct aaacgagatc
    70681 caggatcaag aaaaggttac tttgaaagga atcgtagtct cgcctcctgt gatgaatcga
    70741 tttggttata aaaaaagccg attgcaattt cgtatgatgc aagaacatga tgtgttcaac
    70801 gtttcttttt ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga ggaaatcgcc
    70861 gtatacggta aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat tttaggttct
    70921 caaacaatgg atgacttttc gccgatatat cacgtcaaca aatcaattcg tcaaacgaca
    70981 ttagttgatc taatcaaaaa agggtttcaa gaatacggca cattgattga agaaaatctg
    71041 ccaaacaacc ttgtggagaa atatcgactg cttgaccgag cttctgctgt aagaagtatg
    71101 cattttccaa aagaccacga agaaaaccat caagccaaac gacgtgtcgt ttttgaagag
    71161 ttctttttat ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc agaaacgaat
    71221 ggtttggaga tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa gctacctttt
    71281 gaactgacaa atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct tcgcagtccg
    71341 cagcatatgc agcgactttt gcagggagat gtaggtagcg gaaaaacagt ggttgccgcg
    71401 attgcgttat acgctaccgt tacagcaggt ttccaaggtg cgttgatggt gcctacagaa
    71461 atcttggcac aacaacatat ggaaagcttg caacagctgt ttgatcccaa tgaagtaacc
    71521 atcgcattga ttacggggtc aacgaaagca aaagaacgac gtgagctcct tgaaaaacta
    71581 gagcaagggg aaattaacgt cgtgatcggc acacatgcat tgatccaaga tggagtcgaa
    71641 tttcagaatt taggtctagt tattacggat gagcagcacc gttttggcgt gaaccagcgg
    71701 aaagtacttc gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc gactccgatt
    71761 cctagaacat tagctattac agcatatggt gagatggatg tatctatcat tgatgaactt
    71821 ccagctggac gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt ggacagtgta
    71881 ctggattgga cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat ctgtccgctg
    71941 attgaagaat cagaagcatt agatgtaaaa aatgcagtgg aaatctatga aaaacttcgt
    72001 gaactatttg caccaaaata tgaagttggc ttgttacatg gtaaaatgaa gaatcaggaa
    72061 aaagaagtaa tcatggaagc cttcaaagac aaccagatgc agatattagt ttcgactact
    72121 gtaattgaag taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat ggatgcggat
    72181 cgatttggat tggcacagct gcaccaactt cgtggacgtg ttggacgcgg aagtgatgct
    72241 tcctactgta tcttagttgc taatcctaaa aatgaactag gtgtggaacg gatgaaaatc
    72301 atgacagaga ccaccaatgg ttttgtacta agcgaaaaag atctggaatt acgtggtcct
    72361 ggtgaagtat ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc tgacttggtg
    72421 acggatggaa atgttttgga ggtagccaga gaagaggctg cagccctttg gcaaataaag
    72481 gattggcaga tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat agaaaatcag
    72541 gataatcgtt atttcgatta gcagactgtg ctatacttta tactgatgac ggaaaaagga
    72601 gtggaaacaa tgaaaattgc cgtagatgca atgggcggag atcacgcacc tcaagcaatt
    72661 gtagaaggcg tggctttagc gcagcaagat tttccagaag tagaattctt actttacgga
    72721 aaagaagcag aaatcaagaa atacttaaca aatgaaaaaa acatcacgat tatccataca
    72781 gacgaaaaaa tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa aaagactgca
    72841 tcaatggtcc ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt attttcagca
    72901 gggaatacag gcgcgctact agcagcaggg ttgttcatcg taggacgtat caaaaatatc
    72961 gaacgccctg gcttgatgtc taccttgcca gtagtaggga aagaaggtgc cggtttcgat
    73021 atgctggatc ttggcgctaa cgcagaaaac aaaccagaac atttgttgca atacggtatt
    73081 ttaggttctt tttatgcacg taaagtccga ggtatcggac gtcctcgtgt cgcactctta
    73141 aacaacggaa cagaagcaac aaaaggtagt gaagtgacga aaaaagccta tgaactcctt
    73201 cagaatgaaa caagcttgaa ttttattgga aacgtagaag ctcgtgagct actcaatggt
    73261 gtggctgatg tagtggtcac agatggattt acaggcaacg ctgttttgaa atctattgaa
    73321 ggaacagcaa tgaatatgat gaatctatta aaatctgcta ttctaaatga agggatcaaa
    73381 ggaaaaatgg gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc agaaatggat
    73441 tattccaaac atggcggtgc cgttttgttt ggcttgaaag caccagtcat taaaactcat
    73501 ggagcaacag gccccgatgc tgttcgctat acgatccgcc aaatccacac aatgcttgag
    73561 acagatgtag ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata gtatttggat
    73621 atctgaagcg atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat gccagtaaaa
    73681 ttagccatgg tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa agttgactcg
    73741 cgaagaagta tttaacaaag tagcgaaaat tatttcaaat cattttgaga tagataccga
    73801 taaagtgacg gatgaattga acatcaaaga tgatttaaaa gctgattcaa ttagtattat
    73861 ggaattcgtt ttagagcttg aagatgaatt cggtacggaa atttcagatg aagacgctga
    73921 acaaattgaa acagtcggtg gcgcagtaga ttatatcagc agccatctta aataatttca
    73981 aataaaagca aagaaggata aaaagctgca tgcagctttt tatccttctt tttttaatct
    74041 ttttgacacg taaaagtcat ttttctgata attctggcga taaacagaaa ttatgttgca
    74101 aagtaaagcg gttttctata taattattaa taattcatta cacaaagata agaataacct
    74161 ttgttataaa acgatttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta
    74221 ggaagcagag aggggtattc gattgtccga taaccaatta ttagaagttc agaacctaca
    74281 tacaggattc cgactcaaag atgactatta cgatgcagta gatgacgttt catttacttt
    74341 agacaaaaac gagattttag caatcgttgg ggaatctggg tgcgggaaaa gtaccttggc
    74401 tactacgatt atgggattac ttgatcctaa taacacaaaa attacaggtg agattttata
    74461 taacgatctg aatttaatcg acctgaacga aacgctctat aataaaatcc gcggaaatga
    74521 tatcggaatg attttccaag atccgttgtc tgcattaaat cctctcatga gaatcgaaga
    74581 ccagatcaaa gaaggacttt cctatcacac aaagatgaat gcagaacagc gtcaagctcg
    74641 tgcactagaa ttgctagaac aagtagggat tcctaatcct gcgagagttg gacgccaata
    74701 tccccatgaa ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg caatcgcctg
    74761 caaacctcca atcgtcattg cagatgaacc gacgacagcc ctagatgtaa ccatccaagc
    74821 acagattctt gatttactga aagaccttca ggaagaaaca aaaacaggga tcatcttgat
    74881 cactcatgac ttaggagtcg tagcagaaat ggctgatcga gtggcagtga tgtatggcgg
    74941 acagttcgtt gaagtagcag gtgtaaaaga actgtttgat catcctaagc atccttatac
    75001 acgctcttta ctgaactcta taccacaaga aggaaatcat gatgcagaac tccatgtgat
    75061 cgaaggtgtc gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt ttgcaccacg
    75121 tattccatgg ataccagcaa gcgatcatga agaagagcct atccttcatg aagtcgcacc
    75181 gaaccacttg gtacgttgct cctgctataa gcatttccac tttagagacg aaaaaggaga
    75241 ggtgtagatg acagaattaa tcacaatcaa agacctgaaa gttcattacc ctattcgcag
    75301 tggattcttc aatcgagtaa cggatcatgt atatgctgta gatggtgtgg attttattat
    75361 cgaaaaagga aagacatatg gtttagtggg agagtctggc tctggaaaat ctactacggg
    75421 aaaagccgtt gtgggattag aaaaggtaac agccggacaa atcatgtatg aaggaaaaga
    75481 tgtaacgaag cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc agatgatctt
    75541 ccaagattca atgtccagtt tgaatcctaa aaagcgagtg ttggatatca ttgctgaacc
    75601 catccgaaat tttgaacggt taagcgatca agaagaaaag aaaaaggtaa aaggactgct
    75661 agatatcgtt gggattccgg aagatgcgct atataaatat cctcatgaat tttcaggcgg
    75721 acaacgtcaa cgtctaggag tagcccgtgc tgttgctacc aatccaaaac tgatcgtagc
    75781 cgatgaacct gtatctgctc tggatctatc tgttcaagcg caagtattga atttcatgaa
    75841 acgaatccag caagaatacg gcctcagcta tttattcatt tcacatgatt taggtgtagt
    75901 aaaacacatg tgcgacaata tcgcaattat gtacaaaggt cgatttgtag aaatcggtac
    75961 aagagaagac atttataatg acccgcgaca tatctatacg aaacgtctgc tttcagctat
    76021 tccgcaaatc gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa gagtagaaca
    76081 agagtatatc caaaaccaaa aagaatacta tgatgcgact ggtcgagtct acgatttgcg
    76141 tacgatcaca actacacaca aagtggcgtt gaaagatgga ggtgcaagct aatgtggaaa
    76201 acgattctgc gacgtgtttt attgatgatc ccgcaagtta ttattttaag tgttctgatc
    76261 ttcttattag cccaagcgat gccgggggat ccatttaccg gtttgatcaa tccaaatcag
    76321 gatccggcag tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc atggtatgtc
    76381 cagtacttcc gttggatcgg aaatgctttg caaggcgatt ttggtcaaag tttcttatac
    76441 aaacgctctg ttgctgactt gatcggcgaa cggatcatca acactttata tctatcgatc
    76501 ttgacagttg tccttactta tttgattgct gtgcctctgg ggatgcttgc tggccggtat
    76561 caaaattcaa tcttggataa gattgtcgta atctacaact tctttagttt tgctgttcct
    76621 ttatttatct tcggcttgat catgctattt gtttttggtt atcgtttagg ctggttccca
    76681 acaagtggat cacaaacact aggtttgcaa ggtggattta tcactcaatg gatagaccgt
    76741 ttgaaatata ttctgttacc atcaattacc caagcatttt tggcgactgc cgtcacaatc
    76801 caatatctgc gaagtgaagt aatcgattcg aaatcgctcg attttgtcag aacagcccgt
    76861 tccaaagggg taccgacgaa taaagtattc agtcgacata ttttccgaaa tgcagcgttg
    76921 ccgatggctt ctcaaatggg gtatgaaatc acttcgttga ttgctggttc tgtagttatc
    76981 gaaaagattt ttggttatcc gggagtcgga aaactattta ttgattccat cggccaacgg
    77041 gactataccg tgatcacatc cttagtattg attttaggaa tcgcgacgct agtggggaca
    77101 ttactttcag atatcatcat gagtatcgtt gacccgcgta ttcggattca ataaaaccaa
    77161 tcagaaagga gaatggatcg atgagtgaaa aaaacaatac agcagttcaa gaaagtattc
    77221 ctcctatggg gatacgaatg attgctcgag aatttaaaaa agataaaatt gctatttttt
    77281 cattaggttt gctagtggtt ttattattag tgatctttat cggtgcgatg ctgaccgatc
    77341 aagacaaagt gatgtatgtt agtatcttcg acaaatacgc tgcaccagga acagtttcga
    77401 cacaaggaca aaagttcatt ctgggagcag atgaaggcgg acgtgatgta ttaggacaat
    77461 tgatcatagg tgctagaaac tctgttacga tcggatttgc gatcacgatc cttacttcga
    77521 tcatcggggt tggattaggg atcatctctg gctactacgg agggatcatt gacaacatct
    77581 tgatgcggat cgttgatttt atcatgattc taccgatcat gctgatcatt atcgtcttcg
    77641 tatcgattat ttccagatac aatgtctggt cctttatatt catcatgagt gcattttatt
    77701 gggtagctaa agcccgatta ttccgaagca agacattatc tgaagcaaga agagattatg
    77761 tcagtgcctc aaaaacaatg ggaaccaatg attttaaaat catgttccgc gaaatcatgc
    77821 cgaatctaag ttcattaatc attactaatt tgacgatgaa ctttgcggca aacatcggta
    77881 tcgagacaac tttgactttc ttaggtttcg gcttgccggc aaatgtacct agtttaggta
    77941 cattgatcgg ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg atctggttac
    78001 ccgcatcaat ccttatttta gttttgatgt tgagtatcaa ttatgttgga caagcattca
    78061 agcgttctgc agatgcgcgc caacggttag gctagtatag aagcgaaggg ggaaatagga
    78121 atgaagaaaa cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct tgcagcatgc
    78181 gggggcggag gtacagatac ctctggaaac aactctggag gcaacggcaa taacaaagag
    78241 gaattaactt ttccaattgc aacaaaaaat aatgatgatg caatcgaaga cggcgaatta
    78301 gaagctgcgg tcgcaacgga cagtcagttt aaaggattgt tccagtggga gttttaccaa
    78361 gattcatatg atgcagcttt tatggctcct tctcatgaac cgctatttgc aaacgatgac
    78421 gatttcagaa tcacagacga cggagctgca acgcttgatc tagatgaaga tacgaaaaaa
    78481 gcgacaatca aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt aacggcggac
    78541 gatgtgattt tcccttacga aatcattggg aataaagatt acactggtat ccgttatgac
    78601 gacactttcc gtaacatcgt aggtatggaa gaatatcatg atggaagtgc cgacacaatc
    78661 tccggaatca aaaaaataga cgacaaaaca gttgaaatcg aatataaaga agtaaaccca
    78721 agcatgcttc aatcgggtgg tggtatctgg tcatatgcag caccaaaaca tacgttagaa
    78781 ggtgtagcaa tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt
    78841 ggcccttatt acatgagtaa tatcgttgca ggagaatcag tagaatactt gccaaacgag
    78901 cactactgga atggtacgcc taaattgaaa aagatcacga tgaaatctgt accgatggca
    78961 agtgcaactg aagcattgaa ctctaaatta tatgatatga ttttccaaat gccaacagat
    79021 acttacgata cctataaaga aattgctggc tatacaaact taggccgtga acaaacttct
    79081 tatacttatc taggattcaa gttaggtaaa tggaatgcag atacaaagag tgtggaatac
    79141 aatccagatt caaaaatggc tgataaatca ttacgtcaag caatgggcta tgcattagat
    79201 aataatgcag taggtgaacg tttctatgca ggtttgagaa gcaatgcgac ttcattgatt
    79261 cctccagtat tccagtcata ccatgatgat gagatgaaag gattcacaca agatctagac
    79321 aaagcgaaaa aactattaga tgatgctggc tacaaagatg tggatgacga tggtttaaga
    79381 gaagacaaag atggaaataa actaacaatc aactttgctt cgatgtccgg tggggaaaca
    79441 gcgcaaccat tggcagatta ctatatccaa caatggaaag aaattggttt ggatgtccaa
    79501 tacacaacag gtcgtctgat cgaattcaat tctttctatg ataaattaga aaacgatgat
    79561 cctgaaattg atgtttacca agctgcttgg aatacaggaa ctgatccaaa tccaagtgga
    79621 ctatggggac caaatgctgc gttcaactac actcgttatg aatcagaaga aaatacaaaa
    79681 ttgatcaacg atatcagttc aagcaaagca tttgatgaag agtaccaaaa agaagcatat
    79741 caaaaatggc aacaatatgc atttgaagaa gcatttgcga ttccaacgtt gttccgtaat
    79801 gaagtattgc cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac
    79861 ccatgggcaa atgtttctgt tacagcagat tcacgttcat aatcactaaa aatgaccgaa
    79921 aaagggcgat gtcctttttc ggttttttga tatttttctg tttatttctc taatattcag
    79981 gtcaaggcgc tgatttcatg acttctccaa taaacggtgt tcaaagtcca gtttctcggt
    80041 attaagtcaa atttatgcaa aaatatggaa agccattttc acaaaatttt ttcttcatac
    80101 tcgaaactaa acgaattttt cacaacttct cgtcaatcac taggattatt aaagctttac
    80161 ttaccttttg accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg
    80221 aaaaaatatc cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag
    80281 aaaactataa catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat
    80341 cctatgtgaa tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag
    80401 gagatgccgt tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc
    80461 cagaaggaaa attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat
    80521 ttgccaaaga atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag
    80581 gaggacgtac gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat
    80641 atcttgacca gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag
    80701 tcaaagcagg tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc
    80761 aaaaatcagg tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg
    80821 aacgagtatt ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggga
    80881 aatcgaaaaa attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt
    80941 aagtgaggga aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca
    81001 gacaagacag tcattgattt tgaaaatagc gtcacagcag tggttggacc aaatggcagc
    81061 ggaaaaagca atattacaga ggctatccgc tgggtattag gagaacaatc tgctaaaagt
    81121 ctgcgaggcg gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg
    81181 aatattgcag aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac
    81241 aatgagatca gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat
    81301 aaacaaagct gcagattaaa agatatccaa gaattgttct tagattctgg attaggaaaa
    81361 gaatcgttct caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa
    81421 gaccgtcgtg ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag
    81481 aaagcagaac agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc
    81541 cacgaattgg aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt
    81601 ttacggctaa aagaaacact gactcaaacc gatgtctctt tgatggttgc tgaaatcaag
    81661 acagcaaaaa aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta
    81721 ggcaaactat ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa
    81781 aatgcgcaag ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa
    81841 aaactgaagc agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa
    81901 aaaagctcgc aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt
    81961 gaaaagcttc aagaaagctt gatgaaagca gcagcagaaa aagaaacaga gatccaaaaa
    82021 gctgaagcaa atctcataaa aacacagcaa gaattagaaa aataccaaaa atcaacgaaa
    82081 gaattattgg cagaattaag agatcaatat gttgatttga tgcaagaaca agcagctgta
    82141 ggaaacgaac tgaagtatct agaacgtcaa tatatacaag aaacagcaaa aagcaagcag
    82201 acgttagcca agcaatcgga agtagaagca tcagtagacc gattgatctt acaaaaagaa
    82261 gaattgacac aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta
    82321 gaaatgatcc aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa
    82381 atgtatcaat taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa
    82441 atccaagaaa actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag
    82501 cagctttcgg gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg
    82561 ttagctatcg aaacagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa
    82621 gatgctcggc aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt
    82681 ccactgacga caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct
    82741 gtagaagggt ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact
    82801 gtggttcaaa acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca
    82861 atcgcgcaaa cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat
    82921 gccggaggat ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta
    82981 caaaatcaag aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa
    83041 gcacaagaaa aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa
    83101 gaagttttgc gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag
    83161 ctacaaaaca tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt
    83221 gagacacgtg aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa
    83281 gacaaacaaa cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta
    83341 agtcaagaaa gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa
    83401 gaacaagcag atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc
    83461 ggtgcccgag tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg
    83521 gcgacattga cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa
    83581 attaacgaat tatctgctca gcgagaaaca ttaaaagccg agttggcaaa agccaaagag
    83641 cagcgagata gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat
    83701 cgtgagcaaa aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct
    83761 gaaatgattt tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag
    83821 aaagcttccc aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca
    83881 tccttaaaag aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac
    83941 gaacaagtca gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg
    84001 aaaaaccaat tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa
    84061 gaagtatttg aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc
    84121 ggtaaagctg aattagtact gacggaacca actgatttgc tgaaaacagg tattgagatt
    84181 gaagtacaac ctcctggcaa aaaactgcaa agtctaagtt tgctttctgg aggagaacga
    84241 gcacttacag ccatcgcgtt gctcttttcc attatccgtg tctgtcctgt tccgttttgt
    84301 atcttagatg aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac
    84361 ttgagcgatt tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg
    84421 gtcgcagcag atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt
    84481 tctgtacgga tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa
    84541 cgaaaatgat caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc
    84601 tgatttcaga tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg
    84661 ttctatgtac aggacgcccc ttagctgcga tggcgcatta tctgcaagaa ttaggactgg
    84721 tagatgaagg tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg
    84781 gtgaaatcat ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg
    84841 ctcagcaatt ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta
    84901 gtgccccaag gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag
    84961 cggatataag cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc
    85021 aagaagaatt agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga
    85081 tcatcaaaac aagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg
    85141 gtatttcttt attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcgatcggtg
    85201 atgaggaaaa tgatttaccg atgatccaat atgctggact aggcgtagcg atggagaatg
    85261 ctgtgccttt cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg
    85321 tagccaaagt gatccaaaaa tttgtattag agtaggggga gtcaaatggg attttttgat
    85381 aaaatcaagc gtgcttttac tggcgaagag aaaacagaag aagttgaagt acaggagaag
    85441 tacgacaagg gtttagaaaa gacgcgtaaa acatttggtg aacggatgaa tgagttgttt
    85501 gccaattttc gaacagtcga tgaagacttt tttgaagaat tagaagaaac acttatcggt
    85561 gcagatgtag gttttgaaac agcgatacga attaccgaag cattacgtca agaagtgaaa
    85621 ctcagaaatg cgaaaaaatc ggcagaagtc caaaatgcga tcatcgagaa acttgtggat
    85681 ctctatgaag cggaaggaat caatgaagta aatgaactaa atatccaaga agacggacta
    85741 acagttattt tgtttgtcgg tgttaatggt gtagggaaaa caacaagtat tggtaaactt
    85801 gcccatcagt tcaaaaacga aggtaaaaaa gtattgatgg cagcagcaga taccttccgc
    85861 gctggagcaa tcgaccagtt agttgtctgg ggcgaacgcg ctggtgttga agttgttcgt
    85921 ggcaatgcag gaggcgatcc agcagctgtc gtattcgatg ctttggaaag agcaaagaca
    85981 gagcaggcag atgttttact agttgatact gcaggccgac tgcaaaataa agtgaacttg
    86041 atgaacgaat tagaaaaaat aaaacgagta atccagcggg aattaccaga agcaccccaa
    86101 gaaacacttc ttgtattaga tgcaaccact ggtcaaaacg cgatggttca agcaaaacaa
    86161 ttcaaagaaa caacagatgt gaccggtctt gttttgacaa aattagatgg tacagcaaag
    86221 ggcggtatcg tacttgctat ccgtaatgaa ttgcatttac cagtgaaatt ggtcggtctt
    86281 ggtgaaggaa tcgatgactt ggagccgttt gatccaaatg attttgtcgt tggattgttc
    86341 aaaggattac taaaagaaga ataacccatg tctaaaaagc aggagctgtg acaaaagttg
    86401 cagctccttg tttgaatgat tgtttttaac atagctgtgt ttttgatgcc ttcattttta
    86461 tacttatttg taagcggttc tcaaacaatt tcgttctttt tcttagactt tttgattata
    86521 attagattca tatgaattgg aggtcactta atgatgaatt ttaagcaatt attattgcat
    86581 gtcaatgcgc gcccttttat cgaccaagct cgatttggta tagaacggga aggacagcgg
    86641 gttgatcttg caggaaatct agcaaaaacc gatcatccag caatctttgg cgatcgatcc
    86701 tatcacccct atatccaaac agattttagc gaaacacaaa cagagatgat cacccctgtt
    86761 accgattcta ttcccgaatt atttcagtat ctcgctgctg tttatgatgt gactgctcgt
    86821 tctataccga aagaagagat gatctggcca ttaagtatgc cacctgcctt accggaaaaa
    86881 gacgaagaga tcattattgc aaaattaaaa aatttcgaag atgtcttgta tcgacgttat
    86941 ttagcaaaag aatacgggaa gcgaaaacag atggtaagcg gtattcattt caattttgaa
    87001 tttggcgatg agttgctaag aacattgttc agccatcagg aagaatttca agatttttcc
    87061 gaattcaaaa cagaacttta tttgaaaaca gcgagaaact ttatgcgcta tcgctggatg
    87121 atcacttatt tattcggtgc ttcaccaatg agtgagaaga actacttttt agatgaatca
    87181 catccgcaag aacctgttcg cagtattcgc aacagtgcgc tgggctatac gaatcatcca
    87241 aatgtgaaag tttcctatgc ttctatgaaa cagtatttag cagatatcga gcgaatgatc
    87301 gaagaaggga aactttcgga ggagaaagaa ttttatacac cgcttcgttt ccgaggcgga
    87361 aagaaagtcg cagatttagc aacaacaggt gttcgttata ttgaattgcg gaatatcgat
    87421 ttgaatcctt atgcaagatt gggtatcaat ccagagcaag ttcggttctt acaattattc
    87481 ctgatgtaca tgttatggac agaagaaaaa gaagactgtg accaatgggt agcagaaggt
    87541 acgactcgaa ataacaaagt agcactagaa caaccatcag atcaaacaga atttcatcaa
    87601 gaaggtagag aaattcttga aggaatgaag caaatgctgg ttgaattgga ttggttggat
    87661 tctctttatt tagtagagga agcattgact caaatggatc atcctgaaca aacgttagca
    87721 gcgaaactct atcaagaagc acagctatcg agtcagcaag aagttgctgt ggcattggga
    87781 catcaatatt ataaagaaag ccatgaacgt ccctatcagt tggccggttt tcgcgaaatg
    87841 gaactttcta ctcagatatt tatgttcgat gccatccaaa aaggtgtcca agtcaaagta
    87901 ttagatgagt cggatcaatt tttgcgtctg caattccaag accatgtaga atatgtgaaa
    87961 aatgcaaaca tgacaagtaa ggacagctat atcgtcccac ttatcatgga aaacaaaaca
    88021 gtaacgaaaa aagtcttaaa agaagctggg ttccgggtac caggaggcgc agaattttca
    88081 tccatggaag aagcggtaaa agcttatccg agatttgccg agcaggcatt tgtgatcaaa
    88141 cctaaatcaa ccaattacgg gttaggtatc acgattttca aggaaggagc tagtctggaa
    88201 gattatcaag caggtttagc cattgctttt cgtgaagaca gttcggtttt agtagaagaa
    88261 tttatgccgg gaacggaata tcgtttcttt gtgatcgacg gagaagtcca agccatcatg
    88321 ttgcgagtcc ctgctaatgt tattggtgac agtatccgaa cagtgaaaga acttgttgaa
    88381 gaaaaaaata gtgacccttt gcgtggaacg aatcatcgtg cacctttaga attgatccaa
    88441 ttaggtgaat tagaacaatt gatgttgaaa gaacaagggt tgacgatcga atcagttccc
    88501 caagccaatc aaattgttta tctaagggag aactcaaata tcagtacagg tggtgattcg
    88561 atcgatatga ctgatgaatt ttctgaagct tacaaaaaaa tcgccgtttc tgcggtagaa
    88621 gcactaggag ctaagattag tggtatcgat ttgattatac cagataaaga aatcgatcct
    88681 acaactgata aaaaagcata tgggatcatc gaagcaaatt tcaatccagc catgcatatg
    88741 catgtttatc cttttgcagg aaaaggcaga agattgacga tgaatgtatt gaaattgtta
    88801 tatcctgaag tattttaaaa gaaaaagctt ttgacagatt gtcctatttg atgtcaatct
    88861 gatcaaaagc tttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat
    88921 cccagaagct ttttttcttt tttggttttt ctttttcagc tggcgaagag ttttcttctg
    88981 ggtatttttt ttcgatatca atcacaggtt catttactgc ttcttttgct gttaagagca
    89041 atccgaattc ctcaggattt gcagtgacgt aatcgttgac tacagtgaaa cggctattgt
    89101 ttttcgttgc tagctggata tattggtttt ggacagaaag gggtagagcc ccattcaaga
    89161 gtatggaagc atctggatgt tttttgagtt cttttaaaaa gttttctttg ttttttggtt
    89221 ggatcatttc agcaacagtc atactaagat agcagcgttc cctgaatgtc cctaaatatt
    89281 tatgctgttc ttcagggttc actaaaggtg taccataaag tcccttatcc aaatggcttt
    89341 ttacatcatc ttgtgccata aaaaataccc tccttcttct ctttgtcttt cttacgatta
    89401 tagcatattc ccaataatac ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt
    89461 cacttaaaat aagaatgaaa atcattctca attagaaagg tggattggat gcgctacgga
    89521 cgactagtag cattattagt tattttaagt agtatttcat tgttcatcgg agtatcgaat
    89581 gtacaattgt cagaactgct ccagttagat gggcaaagct tacaaatctt gctagtgact
    89641 cgtttgccac gaacgatttg tttgatcctt gtgggagcga ccagtagtat ttgtgggttg
    89701 atcatgcaac atttgaccca aaataaattt gtttcaccaa cgactgcggg cacgatggat
    89761 agtgcgcgtt taggtatttt agtggctatg attttcctac ctggtgcatc cttgattgtc
    89821 cgatccttga cagcattttg ctttgctttt gcaggaacat tgcttttttt atcccttacg
    89881 cgattgttcc tccagaaaaa tcaattgatg cttccgttga ttggagtcat gttagggaat
    89941 atcattggtt cagttgctac tttttttgct tatcaattcc agttggtaca aaatatgact
    90001 tcatggcttc aagggaattt tgcgactgtg atacgaggaa attatgagtt attgtatgct
    90061 gttgttccat tgttgctctt ggtttggcta tttgcttacc gatttacgat cattggtctg
    90121 ggcgaatcgt ttgcagtaaa tctgggaata gattaccaga agatgcagtt tttaggcatt
    90181 ggcttagtat cattggctag tgcagtcatg ttgatcatgg taggaagcat tccttttatc
    90241 ggagtgatca ttccgaatct tgtctctaga atatatggag atcaagtgca caaaacaatt
    90301 ggcgtaactg ctttagcagg tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg
    90361 ttgatttttc cttatgaaat accagtcagt gtgatcgttg gaatcatcgg tggggctatc
    90421 ttcctttatc ttctgataag gggacgacga tatgcttaaa aaaaagccat ctatttttat
    90481 tggtatgctt gttcttgttt tatttgttac aggaatgtac ttgacgatcc atacatatgg
    90541 aaactggcat tttgcatgtg cattgaggag taagaaaatt tttgcattta tccttgtctc
    90601 gctagctgtc agcagtgcca caatcagctt tcagacaatt acgcataacc agtttttgac
    90661 tccttcagta ttgggacttg agtcgttata tgtccttgtc caaacggtcc ttttcttctt
    90721 tatcggagga gtgacgatgc ttcagcagac aggtacagtt tttttcttat tgaacgttgg
    90781 gctgatggtc gggctgagtc tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa
    90841 tcttttttta ttactgatgt cagggatgat tttaggtacg ctttttggta gtgtcagtac
    90901 ctttttacaa gtcatcatgg atccgaatga atatgatttg cttcaaggca gattatttgc
    90961 aagctttggc aatatccaga gtcagtatct tttgcctgct tctttgatca ttatcggctg
    91021 ctggtacctg ttatttcgat atcgacatgt gctagacgtt ttccatctcg gaaatgaaca
    91081 agcagtaaac ctcgggatag acacagatcg ttttgaattt ttgattttat gttgtgtcag
    91141 tcttttgaca gggacatcaa cggctcttgt gggtccagtt acttttttag ggtttattgt
    91201 cgcaaatatc agttatcgtg tcttttctac ttatcgtcat ggtcagctat ttatcggaag
    91261 ctttctggta gctatgctgt ttctgacagg aggacagctc gcagtagagc agattttcgg
    91321 ctggaataca acagtgagtg tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa
    91381 attgattcgc gaaaggaaga gtatcctatg attgatttac aaaaggtcaa taaactgtac
    91441 catggaaaat cagtggttaa agatgtacag ttaacgattc ctgaaaagaa attgactgct
    91501 tttatcggtc caaatggtgc tggaaagtca acggtgttgt cgatgatcag tcgtttgata
    91561 ccaaaagata caggtgaaat ctatttagac cataacgaag taaaggcttg gaaatccaat
    91621 gaattagcca aaaaattatc gatcctacga caatcgaatg aaataaatgt ccaaatcacc
    91681 gtgaaagaat tagtagaatt tggacgattt ccttatacga aaggaaaatt gacgacaaaa
    91741 gacgaggaaa tcgttcagga atcactggaa caccttgggt tagaagaaat agcggatcaa
    91801 tcgattgaca ctctttcagg cggtcaactc caacgtgcgt atattgcaat ggtgctagcg
    91861 caagacacgg attatatttt actggatgaa cctttgaaca atctagatat gaattttgcg
    91921 gttcaaatca tgcagatttt aaaaaattta gtacaagaaa tcgggaaaac aattgttatt
    91981 gttcttcatg atatcaactt tgctgcaagt tatgcagacg aaattgttgc aatgaaagat
    92041 ggacagttat ttacgcaagg aacgacccaa gaaatgataa aaaaagatgt attggatgaa
    92101 ttatacgaaa tggacatacg gatttgtgag attgaaggcc gcagattctg tctttatttc
    92161 taaaactaac attaggagag aagagatgaa aaaattagta gtaggtatct tgattctagc
    92221 atcatttggt ttagcagcat gtggaaatag caacaacacc agtcaggcag acacaaaaaa
    92281 aagcagcaca caaactgaaa ctacattgac gataacagat agtaatggag accaaatcga
    92341 ggtgccaaat aatccagaaa aagtcgtcgt attcgacaat ggttctttag atacgatgga
    92401 tgctcttggt gttggagata aggtagttgg agcagctaca agcagccttc ctgagtatct
    92461 ttcatcctat aaaaaagtag aatcagcagg tggaataaaa gaaccagact tagaaaaaat
    92521 taatcagctt cagccagatc taatcatcat ttctggccga caaagagatt ttcaaaaaga
    92581 tctaagtcaa attgctccga ccattttcct ttctttagat gcgaaaaatc catgggaatc
    92641 tttccagcaa aatgtcactg ctttagggga gatctttggt aagcaagaag aagctaaaac
    92701 tcaattagag gaattatcta gtgcaatcga tcagacgaaa aagaaagcag aagctactga
    92761 caaaaaagca ctagtcacac ttgtgaatga agggcaatta tcagcctacg gatctggttc
    92821 tagattcggt ttcatccatg atttatttgg ctttgaacaa gcagatgatc aaatcgaagc
    92881 atccactcac ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc cggatatctt
    92941 atttgtagta gacagaacaa aagctattgg tggagacgac agcaaagatg atatttctgc
    93001 caatgaactg gtagcacaaa cgaatgctgg taaaaatcag cagatcatct cccttgaacc
    93061 agatgtctgg tatttaagcg ggggcggttt agaatcaatg aaactgatga tcgaagatgt
    93121 gaatcaagca tttaaatagc ataaataaca gaggattaaa ctctcatact atgtaacaat
    93181 aatgtttctt acattcttaa atagcaagag cctatcctca aagaagttat caaaaaccat
    93241 ccgtaaaatc gtattgatga ttttacggat ggtttttatt tatttgagaa atcttttttc
    93301 aacgtgaatt cattatctac atcatacggg ttgattctag aatggggagt ggcaaaaaat
    93361 agtgtgatcc attttttcag catcaagatc gcctcctttg atttgaatca aaggtacaaa
    93421 gcgaaactta gataaaactt aaatttagat atttttattt tgtaccgatc tcttctttaa
    93481 acgatgttcc atctgctgct tctcggcaat aagtccacta gactagaaaa tatgagaagc
    93541 tattttctgt ccagcgttct tattactcaa agcaaatcgc agttgtcaca atctcttcta
    93601 aacgatgttc catctgctgc ttctcggcaa taagcccact agactagaaa atatgagaag
    93661 ctattttctg tccagcgttc ttattactca aagcaaatcg cagttgtcac aatctcttct
    93721 aaacaatgtt caatcagctg accttcggta ttagctcaaa gcactgattt cacaacctct
    93781 atttaggcat gtgtctcagc aagcggttga tagttgatga tctgcatttt tcgataaaaa
    93841 tcggcgggaa gcttactcca ccgttttaat aattgataga aataactctc ttctgctttg
    93901 attttatact cgcttaatat caatccaaaa ctattcgttt tttttgaagc taatttttta
    93961 taaatatcca atgctttggt tttatcttca ttgacaaaga gtgtttttac ttcaatataa
    94021 gaaccctcga tattttgtgt ttcttcgata aaatagatgt tttttatata ttccttcata
    94081 gtgattcctc caaagaagtc ttttatttga taagtttact ctaccgaaga gttgttaata
    94141 atccgtgaac aaaaagtaat tctttttcca atagttgtta aaggtttttt attttttata
    94201 gtattttttg ttatttaaga aaggaataag gttaaacaaa taacaggttg ctttttgggt
    94261 ctttttccgt tcaagaaact gaacgaagaa aagggaaagt tgtctctttt gtgataagag
    94321 aaatcataat aaacgaatga cttactttcc aataaattga gaacagtcag aaccagacaa
    94381 attagttgca tctgatgaat aaagtgatta ttctatgaca tgaggtgata cagatgaaat
    94441 tagaagtgac aaaagaagca caagaagtat tgaagaataa attagaagca gacgatcaac
    94501 ttttattaga tatcgaaaac ggtgacggtc catttgcaga aagccaagtc agttgccaat
    94561 tggatacggc atttcgattg atcattgtca aaaaagatac acaaacagat ctaagtcttt
    94621 attctgtagt ggctgataca ccagttggac caatcaaaat caaagacagt gcaatacttt
    94681 acatggatga tccatccaca cttgctttag aaccaactta taatagttta caattaaaag
    94741 gaccaagcgg cttgagaaaa ggcaacttgc aagttgttcg aaaagactag agggataaaa
    94801 tgggaaaatt ggcgatcatc agcgacctac atgtggacat caataaattc ggcgaatcag
    94861 agctgatgca gctttggcaa gttttgcaaa agcacaagat cactcggctt catctagccg
    94921 gagatacggc taataaagta gaccgttgcc tagctgttgt cgaatttttc gtcaataaag
    94981 gattatctac gacatttcat ttcggcaatc acgagttggc agatgtgccc aatgaacaga
    95041 tgatggacca ttttccagac ccacattttt taaatgaacg gtatattgct ttaaatgagc
    95101 agaccgttct tttagggatg aatggctggt acgactatca gttttccgaa ttgaaagatt
    95161 acgaggcaat cgtacgaatg aagcgtttgt attggtatga tcggttcatc cagcgtggga
    95221 aaacagatct cgaggtcaat gcagaaatgc ttgcagctgc aaaaaatttg ctagaccagc
    95281 tggaagcaaa aaaacttgag gtgattttat ccactcattt cgttcctaaa cgggcattta
    95341 tcatgtatca atctgctcct tatgagcgtt ggaacaaatt aaatgcattt ttaggatcag
    95401 aatcattcgg agaattactt gaccgttatt caaatatcaa acaagtagtt tttggccata
    95461 cgcatcgtcg ctttgaagat aaatccatcg gtcacacgat ttacagctgc agaccttttg
    95521 gttattatta tgaatggcag ctgacccgag attttgtctt gaaagaacag ttgctagaca
    95581 gctatgatcc tatgaaatta cgtgtcctct tgcgtgccca tcacccagct tttcaggcct
    95641 atcaaacaca acatttgcaa gaggaatttg aacaagcgat gacaatcgtt ccgtattgag
    95701 acagcattag taatgtgtaa aagaaaatac cttgtaaaac ataaggataa gtaagagcag
    95761 tagatccatt tatcgtggat atgctgcttt ttttgcggaa aataatcagc gaatgtttat
    95821 ttgatcggat gatttgtaac tcgcagagta ctagatgcaa gcgtgtattc tttttttgaa
    95881 cgataatcac aaaaaaaaat ata
//
LOCUS       DACDTH010000003        94264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDTH010000003 DACDTH010000000
VERSION     DACDTH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 94264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.003"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWK54_000208"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWK54_000208"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9592362.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWK54_000209"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWK54_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9592363.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWK54_000210"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWK54_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9592364.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWK54_000211"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWK54_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9592365.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWK54_000212"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWK54_000212"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9592366.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWK54_000213"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWK54_000213"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9592367.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWK54_000214"
     CDS             complement(8053..8523)
                     /locus_tag="IWK54_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9592368.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWK54_000215"
     CDS             complement(8569..8994)
                     /locus_tag="IWK54_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9592369.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWK54_000216"
     CDS             complement(9061..9330)
                     /locus_tag="IWK54_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9592370.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWK54_000217"
     CDS             complement(9341..10936)
                     /locus_tag="IWK54_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9592371.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWK54_000218"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWK54_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9592372.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWK54_000219"
     CDS             complement(14489..15049)
                     /locus_tag="IWK54_000219"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9592373.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWK54_000220"
     CDS             15402..16376
                     /locus_tag="IWK54_000220"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9592374.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IWK54_000221"
     CDS             16546..17946
                     /locus_tag="IWK54_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9592375.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IWK54_000222"
     CDS             complement(18024..18746)
                     /locus_tag="IWK54_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9592376.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IWK54_000223"
     CDS             complement(18825..19331)
                     /locus_tag="IWK54_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9592377.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IWK54_000224"
     CDS             19454..21253
                     /locus_tag="IWK54_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9592378.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IWK54_000225"
     CDS             complement(21321..22895)
                     /locus_tag="IWK54_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592379.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IWK54_000226"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IWK54_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9592380.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IWK54_000227"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IWK54_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9592381.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IWK54_000228"
     CDS             complement(24672..25310)
                     /locus_tag="IWK54_000228"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9592382.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IWK54_000229"
     CDS             complement(25311..25961)
                     /locus_tag="IWK54_000229"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9592383.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IWK54_000230"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IWK54_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9592384.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IWK54_000231"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IWK54_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9592385.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IWK54_000232"
     CDS             complement(29104..29844)
                     /locus_tag="IWK54_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9592386.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IWK54_000233"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IWK54_000233"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9592387.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IWK54_000234"
     CDS             complement(31215..32153)
                     /locus_tag="IWK54_000234"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9592388.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IWK54_000235"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IWK54_000235"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9592389.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IWK54_000236"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IWK54_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9592390.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IWK54_000237"
     CDS             complement(35313..35618)
                     /locus_tag="IWK54_000237"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9592391.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IWK54_000238"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IWK54_000238"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9592392.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="IWK54_000239"
     CDS             complement(36724..37008)
                     /locus_tag="IWK54_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592393.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="IWK54_000240"
     CDS             complement(37026..37916)
                     /locus_tag="IWK54_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9592394.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="IWK54_000241"
     CDS             complement(37936..38154)
                     /locus_tag="IWK54_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592395.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="IWK54_000242"
     CDS             complement(38124..39824)
                     /locus_tag="IWK54_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9592396.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="IWK54_000243"
     CDS             40091..40864
                     /locus_tag="IWK54_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592397.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IWK54_000244"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IWK54_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9592398.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="IWK54_000245"
     CDS             complement(41688..42659)
                     /locus_tag="IWK54_000245"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9592399.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IWK54_000246"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IWK54_000246"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9592400.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IWK54_000247"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IWK54_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9592401.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="IWK54_000248"
     CDS             complement(45614..46438)
                     /locus_tag="IWK54_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9592402.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="IWK54_000249"
     CDS             complement(46411..47091)
                     /locus_tag="IWK54_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9592403.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="IWK54_000250"
     CDS             complement(47114..48052)
                     /locus_tag="IWK54_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9592404.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49148)
                     /locus_tag="IWK54_000251"
     CDS             complement(48297..49148)
                     /locus_tag="IWK54_000251"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9592405.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IWK54_000252"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IWK54_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9592406.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="IWK54_000253"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="IWK54_000253"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9592407.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IWK54_000254"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IWK54_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9592408.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IWK54_000255"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IWK54_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9592409.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="IWK54_000256"
     CDS             complement(56026..57267)
                     /locus_tag="IWK54_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9592410.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IWK54_000257"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IWK54_000257"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9592411.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59178)
                     /locus_tag="IWK54_000258"
     CDS             complement(58294..59178)
                     /locus_tag="IWK54_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9592412.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="IWK54_000259"
     CDS             complement(59956..61242)
                     /locus_tag="IWK54_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9592413.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="IWK54_000260"
     CDS             complement(61235..62503)
                     /locus_tag="IWK54_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9592414.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62755..63216
                     /gene="mscL"
                     /locus_tag="IWK54_000261"
     CDS             62755..63216
                     /gene="mscL"
                     /locus_tag="IWK54_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9592415.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63276..63722)
                     /locus_tag="IWK54_000262"
     CDS             complement(63276..63722)
                     /locus_tag="IWK54_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9592416.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63815..64060)
                     /locus_tag="IWK54_000263"
     CDS             complement(63815..64060)
                     /locus_tag="IWK54_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592417.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64175..64753)
                     /locus_tag="IWK54_000264"
     CDS             complement(64175..64753)
                     /locus_tag="IWK54_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9592418.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64830..65360)
                     /locus_tag="IWK54_000265"
     CDS             complement(64830..65360)
                     /locus_tag="IWK54_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9592419.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65364..66224)
                     /gene="rlmB"
                     /locus_tag="IWK54_000266"
     CDS             complement(65364..66224)
                     /gene="rlmB"
                     /locus_tag="IWK54_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9592420.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66217..66612)
                     /locus_tag="IWK54_000267"
     CDS             complement(66217..66612)
                     /locus_tag="IWK54_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9592421.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66609..68021)
                     /locus_tag="IWK54_000268"
     CDS             complement(66609..68021)
                     /locus_tag="IWK54_000268"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9592422.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68018..68554)
                     /gene="cysE"
                     /locus_tag="IWK54_000269"
     CDS             complement(68018..68554)
                     /gene="cysE"
                     /locus_tag="IWK54_000269"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9592423.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(68876..70336)
                     /locus_tag="IWK54_000270"
     CDS             complement(68876..70336)
                     /locus_tag="IWK54_000270"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9592424.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70506..71663)
                     /locus_tag="IWK54_000271"
     CDS             complement(70506..71663)
                     /locus_tag="IWK54_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9592425.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71697..73067)
                     /gene="radA"
                     /locus_tag="IWK54_000272"
     CDS             complement(71697..73067)
                     /gene="radA"
                     /locus_tag="IWK54_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9592426.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73130..73657)
                     /locus_tag="IWK54_000273"
     CDS             complement(73130..73657)
                     /locus_tag="IWK54_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9592427.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            73887..74693
                     /gene="proB"
                     /locus_tag="IWK54_000274"
     CDS             73887..74693
                     /gene="proB"
                     /locus_tag="IWK54_000274"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9592428.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            74690..75937
                     /locus_tag="IWK54_000275"
     CDS             74690..75937
                     /locus_tag="IWK54_000275"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9592429.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76013..76357
                     /locus_tag="IWK54_000276"
     CDS             76013..76357
                     /locus_tag="IWK54_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592430.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76474..76800)
                     /locus_tag="IWK54_000277"
     CDS             complement(76474..76800)
                     /locus_tag="IWK54_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592431.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77415..78617)
                     /locus_tag="IWK54_000278"
     CDS             complement(77415..78617)
                     /locus_tag="IWK54_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9592432.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            78734..79249
                     /locus_tag="IWK54_000279"
     CDS             78734..79249
                     /locus_tag="IWK54_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9592433.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79448..79894)
                     /locus_tag="IWK54_000280"
     CDS             complement(79448..79894)
                     /locus_tag="IWK54_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9592434.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(79941..81419)
                     /gene="msr"
                     /locus_tag="IWK54_000281"
     CDS             complement(79941..81419)
                     /gene="msr"
                     /locus_tag="IWK54_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9592435.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(81773..82402)
                     /locus_tag="IWK54_000282"
     CDS             complement(81773..82402)
                     /locus_tag="IWK54_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9592436.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82386..82850)
                     /locus_tag="IWK54_000283"
     CDS             complement(82386..82850)
                     /locus_tag="IWK54_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9592437.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83337..84605)
                     /locus_tag="IWK54_000284"
     CDS             complement(83337..84605)
                     /locus_tag="IWK54_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9592438.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(84836..85573)
                     /locus_tag="IWK54_000285"
     CDS             complement(84836..85573)
                     /locus_tag="IWK54_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9592439.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(85640..86209)
                     /locus_tag="IWK54_000286"
     CDS             complement(85640..86209)
                     /locus_tag="IWK54_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9592440.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            86337..87209
                     /locus_tag="IWK54_000287"
     CDS             86337..87209
                     /locus_tag="IWK54_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9592441.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            87551..88423
                     /locus_tag="IWK54_000288"
     CDS             87551..88423
                     /locus_tag="IWK54_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9592442.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            88420..90378
                     /locus_tag="IWK54_000289"
     CDS             88420..90378
                     /locus_tag="IWK54_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9592443.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(90710..91561)
                     /locus_tag="IWK54_000290"
     CDS             complement(90710..91561)
                     /locus_tag="IWK54_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9592444.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            91684..92151
                     /locus_tag="IWK54_000291"
     CDS             91684..92151
                     /locus_tag="IWK54_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9592445.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(92414..92896)
                     /locus_tag="IWK54_000292"
     CDS             complement(92414..92896)
                     /locus_tag="IWK54_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9592446.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(92996..93859)
                     /locus_tag="IWK54_000293"
     CDS             complement(92996..93859)
                     /locus_tag="IWK54_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9592447.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        26337 a        20305 c        16934 g        30688 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    62761 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    62821 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    62881 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    62941 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63001 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63061 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63121 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63181 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaaagaaag
    63241 ccgattttca gtgtcggctt tcttttttta gattcttaac ttgctacttg ttcctttttc
    63301 atgtcgatat gtggaatatc atcttccaaa tacacatctg agattgcctt gaatccaaac
    63361 gactcataaa aagcttgtag gtattcttgt gcgccgatca cgattggttt ttctggaaaa
    63421 tttttctgga tcatgttaat cgtttcttga acaatctttc ttcctaaacc atctttacgg
    63481 ttgtcctgat gaacaaggac acgtccaaaa tggatactct ccgcttcttc atatactcgc
    63541 gtgtaggcaa ggatcttccc atcagtatct tgaaaaaacg tatgccaagc ttctccatca
    63601 atgtcatcaa cttcttgata aggacactgt tgttccacaa caaaaactgc gattcgttct
    63661 ttgacgatcg cgtaaaactc tgctgttgac aattcttcaa atttctttac ctgatattcc
    63721 actaagcaaa cgccccctgc ttttttattc ttagcatacc gattcgcaca caaaaaagca
    63781 aacgaacctt acgaatctca ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga
    63841 tctcaactgt ccgagtaagt acatctgaat agctgtaaga tactcgttca aaagaatttt
    63901 cttcaggatc caaatcaaca acgaatactg aaggataggt ttctgttaaa acgccgcggc
    63961 gttctgtttg acgctttctt cctgtctgag caaccaacat gattgggctg ccgatacgac
    64021 cttctaaatc tttcttgatt gaagctaaag ttgttggcat acagttcacc tctactatca
    64081 actataccac atatttttaa aattttcaag tttaccataa aaaaaaaaga aatgcaataa
    64141 aaataaataa gaagctcatt aagatttttc taaattattc ctcattataa aatttcaagt
    64201 gtttttttat tttgagtttt actctcccgt aagcgctcat tacttgcttg tgatccattg
    64261 ctaaacattc agccatgtct acaaaatcaa cgccttgcat ataataatga aaaattttag
    64321 cttctatttt tgaaaactgg atttcgcaat tttcgattgc ttcttttaat aaaagatgct
    64381 gaattgctaa aggttcttgc aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta
    64441 ttttttggtc gattgagact tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc
    64501 tcaacagaga atagatttga ttgcttataa cgcttttata ataacttcca aagctaacac
    64561 ctttgctttt ttcgtaagtt ctcaatgcct gatataaagc gatccttgct tcttgttccc
    64621 aatcctcaga aacataattt cttaaataat aggtgttttt caaacgtagt aaaagtggaa
    64681 aatatttttg atacagggca gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata
    64741 actcctcaga cataaaataa ttcctccata aattcgttta tggaggaatc ttatcatcaa
    64801 tgattcatcg aactgattag aacgatatgt tatttatttt ttctcattaa atcgtccaat
    64861 ttttgggaca atttttttaa ctgctcttca ttccatggag aattacgtcg gaaatcaata
    64921 aaatgcatat ctttattttc ttgggctatt gttttttccg ttttttttat cgtcttatat
    64981 aattcataag cagaagttcg taatgctcct tgggaaaaga tcatccactg ttctgctaaa
    65041 tcgcttgtcg ccactgtcac ttgtgtcaaa cggtcattca gctgaccagc gattctctcg
    65101 atataactgt cagccgtttc gtcttctttc gtaaaaacaa catccaactg gtatttctta
    65161 taattttgtt ggatgcccgg aactagttgg gcatcgaaga caacgatgac ttccattcct
    65221 tcatatttcg cataatttga taaacgatgt aaaagagctt ctcttgcatc cgccattttg
    65281 tcctgctttt taagctggac aagttccggc cacgaaccaa tcatgttgta gccgtctaca
    65341 atcaacagct gttttttcat ggtctactcc ccctgacgtt gggaaaaagc ctgatacata
    65401 agcaaaccag cagcaacact ggcatttaag ctctggacgt gcccggtcat cgggatcgtc
    65461 agcaattcat ccatttcttt tttcaatcct ggactcattc ctttgccttc attaccaatg
    65521 actaaagcaa ttgatccttt ggcattccat ttttggaagt ttgtgccggt catatctgtg
    65581 ccaaataccc aaaatcctgt ttctttgagc tgttgcacag cttgtaccaa attcgtcacg
    65641 cgtgcaacag ggacatgttc gactgcccca gtggaagtct ttgttacgat aggtgtgatt
    65701 ccaactgctc ggtgttttgg tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt
    65761 aaaatcgacc caaaattgtg tggatcttcg atgctatcca aaatcaaaaa gaaggggtct
    65821 tcttttttct cttttgttcg ttcaatcagt tggtcaaggg ataaatattc ataaggtgta
    65881 atcgcaagaa cgactccttg atgcacgccg tgatcgctca aagtttctaa tttttgttta
    65941 ggaacccatt tgactggcac agcttgttca cgtgcgattt ctttcagttc ttctactttt
    66001 tctcctcgtg aatcttcttg aagaaagagt ttattccctc gcccttgttg tagcgcttct
    66061 actgcggcat ggtgcccaaa gacgaaatta tcttctaatg cttcttcatt ttgatcagaa
    66121 actgactgat ttcgtccagt ttgcttttta tttgaatatc tattttgttt tttgtcttca
    66181 aatccacgtt tcgattgatg tttgtttgtt ctttctttac ccattttttt caccaacttt
    66241 ctgaatacac cagcggatca attcttccat ccgctcggtt tgttttgtca aatgcagata
    66301 gcccatgagc gcttcgaacc ctgttgcgac ccgatacgtc gtaacatcag cattttttgc
    66361 gctcgtatgg cttttagcat ttcttcctct gcgataataa agttcttctt cttctgtcaa
    66421 aagatgttct tccagcattt gctgaatcaa aaaggcttgt gcattcgcag aaacataatt
    66481 cgttgccgca cgatgcagct tattgggttt tgtttctcct tgatcaatca ggtggtcacg
    66541 aacatagatt tcgtaaatcg catctccaac ataagccaag gctaaaccgt tcaattgtgt
    66601 gtaatctctc atgcttctct tctccatcgg gttccttgcg gtgtatcttc taataagatc
    66661 cctttttctt tcaacaaatc acgaatttcg tcgcttcgga caaaatcacg attgcttctt
    66721 gcttgatttc gttcttcgat caattgttca atctcttcat ctaataactc ttctgctgta
    66781 aataaaatac cgaagatcga aagccattgc gagaataaag cttctccttg tgccaagaca
    66841 gcggcagaaa ctttcggctg gtctagataa gtgttcagcc acttagcaag ctcatagacc
    66901 actgtgatcc catttgctgc attaaaatca tcgtccattt cttcgatgaa tcgctgttct
    66961 aaattttcta acaaagcgat tttatccgca tcatctgcca aattctctga ggcagtttct
    67021 tggcggaatt tcaaattgtc aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct
    67081 ttcatcgtcg tttcactgta gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa
    67141 cgcaagactt gtggatcaac gtttttgatc atttcatgta cagtgatgaa attccctaga
    67201 gatttactca ttttttcatc gtcttcgcca atcgttacat agccattgtg catccagtaa
    67261 ttagcgaatt ttttccctgt tttcgcttca ctttgtgcga tttcattttc atgatgagga
    67321 aattctaaat cttgcccgcc accatggata tcgatcgttt ctcctaaatg ttttgttgcc
    67381 ataacggaac attcaatatg ccaaccggga cgtcctttgc cccatggtga atcccaagaa
    67441 atctcgccag gttttgcttg tttccataaa gcgaaatcta atgggtcttc tttttgctgc
    67501 tgttcttccc ctgtacgctg acttgctccg acttccagtt catcgatgga ttggtcactt
    67561 aatttcccat aattttcaaa tttacgggta cggtaataca catcacctga agattcataa
    67621 gcatagcctt tttcaaccaa cacttgaata aatgctaaaa tatctggcat atgatccatg
    67681 actcttggat gcagtgtagc aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct
    67741 cggatgaaac gatcagcaac ttctggtgct gtaataccta attcgtttgc tgtacggatg
    67801 attttatcgt ctacatccgt gaaattcgat acgaaatcga ctttatagcc tcgatattca
    67861 aaatagcgtc gaattgtatc gaaagcaatg gtgctacgag catttccgat atggatataa
    67921 ttatacacag tcggtccgca gacatacata ccgacatgtc ccttttgtat agggatgaat
    67981 tcttcttttt ttctggtcaa tgtattgtag atttttatca ttctgatctc ctcacttttt
    68041 ctcctttgat tcgaacgact tttgctggta ctcctaccgc tgtagcgtcg tctggaacat
    68101 ctttcaacac aacggcacct gcaccgattt tggcgttttt tccgatcgtt accggtccta
    68161 atatctgcgc attcgcagag agcatggctc cttgtttaac agttgggtgt cttttacccg
    68221 tttcttttcc tgtgccgcct aatgttacgc catggaataa aatcacatcg tcctcgattt
    68281 ctgcggtttc gccaatgacg attcccattc catggtcgat gaacacgcca gttccaatcg
    68341 ttgctcctgg atggatctca acaccagtta ggaaacgcca gaattgagca ttgatcttcg
    68401 ccaataaaaa gagccggtgc tgatataaaa aatgagagaa acgatgccaa aacaaggcat
    68461 gaaaaccagg gtatgtcaaa acgatctcag ctgtcgaacg tgcagccgga tcgtttctct
    68521 ttgctgcatc aatcgctcgg gtaaaccaac tcatgacttt ccactccttt cgtaagaatt
    68581 ttatgtagga aatcatcttc ttataaagaa aagaagcgcc ttttaaataa gtttccttat
    68641 caaaagacgc tttccgcgtg gttccacttt tcttcataat caatgattat ctctgacagc
    68701 taacgcctgt tatacgttct tggctacttt gtttcctata aaacagtttt tcctgttttc
    68761 tgtgtttcgc caagaccact cccagaggca tttcggcaac cattcattga taccttgcac
    68821 cacccggtat ctctcttgta atgatcagct gcttactttt ttctgttcat cgtgtttagt
    68881 tcagtacttg atttaaatgt gctaatgatt tttcacgacc aagcaattcg atcgtttcag
    68941 gcaattctgg accatgcatt tgtcctgata cagctacacg gattggcatg aaaagatttt
    69001 ttccttttac gccagtttct ttttgaacag ctttgatggc agctttgaca gttggagcat
    69061 ctactacttc catttgttct agctgtgctt tgaatgcgtg gagaacagta ggaactgttt
    69121 caccagcaag tacttctttt gctgcttcat tcaatacagg atgttcattg aaaaatagcg
    69181 tagacagatc aacgatttct gcagcatagc tcatttgagg ttgatacagg ctaacgatct
    69241 tcttcaccca ttcgattgtc ttttcatctg ggttttctgc tacacgtcca tcagcgatta
    69301 gataaggcag acacatgtca gtaaatgcat ctagatccat tgctttgatg taatggttgt
    69361 ttacccattg cagttttttc gcatcgaaag ctgcgggtga tttacttagg cgttttggat
    69421 cgaacatttt gatcaattct tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc
    69481 ccaataatgc aataaagttg aacattgcat ctggtagata gcctaattcg cgatattgtt
    69541 cgataaattg caagattgat tcgtcacgtt tgcttagttt tttacctgtt tcactgttga
    69601 tgatcaatgt catatgtccg aaggttggtg gtgtccagcc aaatgcttca tagatcatca
    69661 gttgttttgg tgtattggca atatggtcat ctccacgtaa aacgtgagtg attttcatca
    69721 aatgatcatc gaccgcaaca gcaaaattat aagttggcat accatcgcgt ttttggatga
    69781 caaagtcgcc accgatatta tcggattcga acgaaatagc gcctttcacc atatctgtaa
    69841 atgcgtattc tgtattgcga ggcacacgga aacgtacaac agattctaga ccttgtgctt
    69901 ctttttctgc ttgttcttca ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt
    69961 ctcctcttgc gcgttgtgct tcccgttctg cttctaattc ttcttctgtg caatagcatt
    70021 tgtatgctcg attgctagca agcagctgat caatcagcgg ttgatagatt tctttacgtt
    70081 cagattgacg gtaagggccg tattctccag gattttcagg agattcatcc caattcattc
    70141 ccaaccaagc aaggttttcc aactggcttt tttcgccatc ttcgatatta cgtttttggt
    70201 cagtatcctc aatacgaata ataaagtctc catcattgtg gcgggcaaac aaataattga
    70261 ataatgcagt tcttgcattt ccaatatgca ggtgcccagt tggacttggt gcatagcgta
    70321 cgcgtacttt cgtcattttc ttcctcttct ttcaaactag actttgacta acagcaaaaa
    70381 ttccgtcagt tacggcttct caaatatcat ttatttaaag ccactcagca caaattttac
    70441 cttttctaag gtaaatagta aagccaactg gttgaatttc cttaccagtt ggctgaattt
    70501 cttttttatt ttgattgttt agtctttttc tctgtctctt ttttattgtc gattcctcgt
    70561 cctgcatgtg caggtttagc aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt
    70621 acaacgactt cgatacgttc attcatgtaa tgctgtccat cttctacgac aaccatcgtg
    70681 ccgtcatcaa ggtaggctac tccttgttgg cgttctgtcc cggcttttac tactagaaca
    70741 ttcatcgttt caccagggat gacgactggt tttacggcat ttgccaacgc attgatatta
    70801 agaacaggta cattttggaa ttcagagact ttattcaagt tatagtcatt ggttacaatg
    70861 acaccatcta aaagcttagc caatttgatc aatttgctgt ctacttcact gatatcttca
    70921 aagtctccat cgtacatttc gacagatata ccctcttctt tttgcaaggc atttaggata
    70981 tctaatccgc gacgaccacg cacacgtttc aaactatccc ctgagtccgc gatgtattgt
    71041 agttcgtaaa gaacaaaatt agggatcatc aaggttcctt ctaagaatcc agtctttgta
    71101 atatcataaa tccgaccgtc aatgatcacg cttgtatcca agattttata tttatggaaa
    71161 ttttctcctg attttcgttc taacagttga ctctcaactt gactagctgt ttcttgctcg
    71221 atttttttgc ttcgtgaacc aaaaactttc ttccattctt cgattcgtgt cgttcccatt
    71281 ctcaatccca gatagcctaa gatgatcatc agtaaaatcg gcaatacact attgacgaat
    71341 ggaatttcca tattgtaaag aggggtcgag atcacaacac ctaaagtcaa tccgatgatc
    71401 gcaccgatcg caccgaagag aagataagtc aagctgacct cacttagttt ttgttctact
    71461 tgtttgataa tagctgagat atgtttggcc aacagcaaag ataataaaaa gaaaataagt
    71521 gcgcctaaca ggctgttggt aaatgtatta ttcagccatc cgtttgactg ttgaccgatt
    71581 gcctgccaag ctgagggtaa aaatgaaata cccaagcttg cgccgacaat gatcatcagc
    71641 aaagtaatga cacgtttttg catcgttttt cctccgtttc gacaatcatt tattagttag
    71701 cggaacacac gcttcaacgt ttcagccaaa gttgctactc caactatctc gattcctttt
    71761 ggcgcttccc agttacctaa gttgttttta ggtaggtata cttttgtgaa gccgagtttt
    71821 tggacttctt tgactcgttg ttcaatatga ctgacacgtc tgatttctcc agtcagtcca
    71881 atttcaccga tgaaacattc cgttggctgt gttccttttt ctttatagct tgaagcaata
    71941 ctgacagcga tagctaaatc aatcgctggt tcatttaatt ttaccccacc tgcagctttt
    72001 aagtaagcat cttgattctg aagaagcaac ccagctcttt tttccagcac tgccatgatc
    72061 agagataccc gattgaaatc aagacctgtt gttgttcgtt tggcatttcc gaacatagta
    72121 ggtgtgacta atgcttggat ctctaccaaa attggacgtg tcccttccat tgccacaacg
    72181 atagcagagc cagttgcacc gtctaggcgt tcttctaaga aaatctgtga aggattagcg
    72241 acttcttcca atccatgctc atgcatttca aaaatcccaa tctcattcgt tgagccaaat
    72301 cggtttttta ctgctcgcaa aatccgaaaa ctatgatgtt gttcaccttc aaaatataac
    72361 accgtatcca ccatatgttc taacattctt gggccggcga tcgagccttc tttggtaaca
    72421 tgaccgacga taaaaatggc tatcccattt gttttagcgg ttttgagtag ttctgcagtg
    72481 gtttctctta cttggctgac acttcctgcc acacttgtga tgtctggctg cgtcatcgtt
    72541 tggatggaat caatgataac ataatcaggg gagatatgtt cgatggcacg actgatttcg
    72601 ttcatatctg tttcagcgta tagataaaat tctgtgttga tacttccgag gcgttccgcg
    72661 cgtaatttta tctgctctgc gctttcttcg ccggatacat ataagacttt tccgcctatg
    72721 gctgctaatt gctgagaaac ttgtaacagc aaagtggatt tcccgatacc tggatcgcca
    72781 ccaatcaaga ccatagaccc tggcaccaca cctccgccta acacacggtt cagttcttct
    72841 aattttgttt tgatccgagg ttctttttta gggacgacgt ctgcgatttt tgttggtttt
    72901 gctttttctc ctgtcaaact tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct
    72961 tcgaccatcg tattccattt tccgcaattt gggcagcgtc ccaagaactt aggcgacaca
    73021 tacccgcaat tttggcaaac aaattgtact tttgattttt ttgccattgt accgcctcct
    73081 tttatcctaa tcatgagaca ttaaatcaag cttaagtatc tttaacgttt tatttactgg
    73141 aagaaccaaa tccgccttga cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg
    73201 gcaaaaagat cccttggccg atacgctcgc cttttttgat cactacatcc ttgaaaccaa
    73261 agttggtgaa ttggaacatg atatggcctt catttgcctc attattgtaa taatcactgt
    73321 caatcacacc tactccgttt gtcaacacta aaaaccgctt caacggattg cttgaacggt
    73381 tagcaagctg caaaaattca tcttctccca tgtaagcttt gattccagta ggcacgaggg
    73441 ttggtttcaa ttgtttttca ttttctgata catctttttt caatccatct gcgactgttt
    73501 ccaacccctt tttccagcct gctttccaga tacttggaat cacgacctct tctgctgctt
    73561 caaaatcata gccagcagca tgatgtgtag cgcgctgcgg tagattgata ttttgatctt
    73621 tatatttaga aataacttca aatcctcttt ttctcaccgg ttttcctccc taaataaact
    73681 actcctattc taacatatcc gaggctgtaa aagactttct ttacaaattc ttcaagaaat
    73741 tctgctatct taaagtaaac cattaattcg ttctttttcc aaacgactgc aagttgtaca
    73801 aaaaaactat tttttacatg actttactcg tcaaaacaag tagaatagaa cgaaattaat
    73861 gagtggatat ttgattgagg tgtcacatgc gtaaagaaat taaaaatgca aaacgaatta
    73921 ttgtcaaagt aggaacaagt acattgattt atcccaatgg aaatatcaat cttgcggcaa
    73981 tcgatcagtt agcttttgtc ctttctgatc ttagaaatcg gggaaaggaa gtgatcttag
    74041 tctcatcagg agctgtaggc gttggtatgc ataaattaca tttgtcagaa cgtccgactt
    74101 tgattcctga acagcaagct gcggcggctg ttggtcaagc cgaactgatg aatatctata
    74161 atcaaagatt cagtgtatat aatcaacaga cggcacagat cctcgtgaca agagacgtgg
    74221 tagattatcc tgaaagtcgt aaaaatgtga ggaatacatt ggaacagctt ttgattatgg
    74281 acacgatccc tatcgtgaac gaaaacgata ctgtagcagt ggatgaatta gatcatctga
    74341 caaaatttgg cgataatgat cagctgtcag ccatcatggc aaaacttgtc gcggcagacc
    74401 ttttgatcgt cctttctgat atcgacgggt tttattcaga taatccgact acaaatcccg
    74461 aggctcagat gtatcgctat atcgataaaa tcgatgaaaa gattctggca caagcaggtg
    74521 gtgctggtag cacatttgga acaggcggta tgcagagtaa attaaaagcc gcttctagga
    74581 tatttgaagc aaacagcgca atgattttag caaatggaaa aaatccggca attatttttg
    74641 atattcttgc aggaaaagaa gtaggtactt tatttaagga ggacttgaaa tgactgactt
    74701 gatgcaatta ggacaacagg caaaagatag cgcgcaacaa ctagcactta tgcctacaaa
    74761 ccgcaaaaat gagctacttg ttcaaatggc acaaaccatc aaaaagaatc aacaagcgat
    74821 cattgaagca aatcataaag acttagaaaa agcagtcgaa aacaatatca gtgaaacgat
    74881 gcaagaccgc ttgcagctga cacctgagcg aatcaatgcc atggctacag agatcgaaaa
    74941 aattgcttcg ttagacgatc ctattggaaa agtagatgag atgtggacga acaccgacgg
    75001 attgagaatc ggcaaaaagc gcgtgccact aggcgtgatc ggcatcattt acgaatctcg
    75061 tccaaatgtc acgacagatg cagctagtct agcattcaaa tcagggaatg ccgttatcct
    75121 tcgcggtggc aaagaagcat tcttctcgaa tcaattatta gtccaattat tgcagcaagt
    75181 actggtcagt gctggcgaat ctccttatgc catccagttt gtcgatgata cttctcatga
    75241 aactgcagga cagttgatgc gattgacaga atacttagat gtgctgattc ctcgaggcgg
    75301 agcaaaatta atccaacgag tcaaagaaca ggcaacggta cctatcattg aaacaggtac
    75361 aggaaataat cacgtatata tcgataaaga agcacagtta tctatggcag tgaacatcat
    75421 cgtcaatgca aaagcttccc gtccatctgt ctgtaacgcg gcagaaacat tgctgatcca
    75481 ttctgagatc gcaccttttt tcttgccagc tattgaaaaa gaactggtag aacacggggt
    75541 ctctttacgt gcggatgctc gtgcgttaga atatctagag acggctgctt tagcagaaga
    75601 cacagattgg gatactgaat atctagatta tatccttgct gtcaaagtgg tcgattcttt
    75661 ggatgaagca attgcacata tcaatcggca taatactaaa cattcagaaa cgatcgtgac
    75721 agatagttat gcggctagtc aacgtttctt aaatgaagtg gacgcggcgg ctgtctatgt
    75781 caatgcctcc actcgtttta cagacggatc ggtatttggt tttggtgcag agattggtat
    75841 ctctactcaa aagttgcatg cccgtggtcc aatggggtta aatgaattaa cttccacaaa
    75901 atatatcatt tatggcgatg gacaaatccg cgaataatgt ttcgtgtgaa acattattcc
    75961 cgccttttgc cttttttctg taaaggttct atccaactag aaaggtgtca atatgtttga
    76021 aattttaatg atttttttta tttaccttat ttctctgaat atcgctgctt ttctgggagt
    76081 cggtatttta tcccttttct ttcaattcaa aaaacgaagc atagggagtc aacgggaaaa
    76141 atggtcgcag tattttgaca aaatcgggca aaaagggctg gttacacgct tgcacattag
    76201 ttacatggtt gcattatgct tattagcgac aatcaattac tattcttttt tcgatcattc
    76261 aatcgcttat acgattacgc tactgattgc tgggattttt cacttatctt ataaatatca
    76321 gctgaataaa aaccacttga atcggacttt ccgctaaaaa atatccgtac gataagtaga
    76381 gtgccttgaa gatcggcaaa aaactgccgg actttttaga ggctgtgaca aagattttgt
    76441 cacagcctct ttttccatat aaacggcgtt cgcctaccag ctgcctggta aatctgtttc
    76501 ctcttcgtta agaagcgaaa atccgtcaaa tgtttcgcca tcataataac gaagcagctc
    76561 atccatggac aagttcatcg ttttttcttt ttcttcttcc gctgattcag ccagtagctg
    76621 ttcccgatac acctgataca taccagctat tttttgctgc atcgcttttc tagtttcacc
    76681 ttccgcattg attattggca attctttgat atagccacga taaatcaacc gatcatctga
    76741 aaattcaatg ggtaattcaa cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat
    76801 ctattatccc cccattcttt ttcttaaatg aattttagct tacctactga aaattttcca
    76861 gtttcattat tttgagaaaa aaagggaatg tgacaaaaat cgtgtcacat cccctttttc
    76921 cgaataaatg gtgttcttct gccaactcct caatcataag tcacaatatt caacaaacat
    76981 gagaaactgt tttttgaaat tgttccttgt gattcagagt taaacgacag tctcacaacc
    77041 tcttggtaaa cggcgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggtaaaata
    77101 tgagaagcta tttttccgtt ttgtttctct aatacttagg ataaaacgct gattttacaa
    77161 cctctgttaa acggtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat
    77221 atgaggaact atttttccgt ttgtttctct aatactcagg acaaaacgct gattttacaa
    77281 cctcctgata aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa
    77341 aatatgagaa gctatttttg tttggctgtt cttatgactt gatgcaggac agctttttca
    77401 caacctctga tttcttattc ttcttcattt gtagcaaaat aattacggtg ataaatgata
    77461 caacatacac tgataagaac aatggcacct gcaaaataaa acgcatagct ttcaccaata
    77521 tgttcaccaa tcacacctgc aataaatggt gctaaagctc caccgaaaaa gcggacaaaa
    77581 ctatagctgg acgaagcaac gtttcgttgc agacctggta tttccatagc aatcgtcgtc
    77641 aacagtgtat tgataattcc ttggaaaaat cctgctaaca caatgctgat cggtacaagg
    77701 actggcatag aagttccagc tcctaataac agcaaacaaa taatgaataa gacaaatgct
    77761 gctaaaattg tccgataagt cgtaaacttc cgctcaagag ccggtgcaac gaaaatcgaa
    77821 gcaatagcca gaagcacacc ccagccaaaa aatacaaaac cgacttctaa ttcagaatag
    77881 ccaatcaata aaaatggcgc atacgcaagt aaagtaaaga atccgaaatt atataacaat
    77941 gcgattagtc caactgaacg aagtttatga ttgcttaacg ctttgacacc ttcccaaagg
    78001 cctacttttc tctttggttt ctcagtttca ggtaaaagta ccgctagggc tataaaaccg
    78061 caaaacatca aaaatgctac cccaaaaaat ggataacgcc atgaaccact gcccaagacc
    78121 ccaccgacta gagggccaac agccattccg cagccaagtg ctgcttcata catcatgatt
    78181 gctttttctg tgttaccaac taaaatactg acgatcgttg ctaaagcagt agagacaaat
    78241 aaagcattcc cgacacccca gccggcacgt aacccaatca gcattccgat attcccagat
    78301 aatccgccaa gaccagcgaa tacaacgatg ataaacaatc ctgtcatcaa tgtttttttc
    78361 aaaccgatcc gactagataa gaaacctgta aatagcatca ccacaccagt gactaacatg
    78421 taactagtaa ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt
    78481 tttaagattg ggtctactag acccactccc ataaaagcga tcatacaggt agctgctaca
    78541 actagcgctg aacttttccc ctttgtttct tcatttgttt gttcttgtat atctatttga
    78601 ttctccaata tttccatccc tttctctcta ttagcatcat acatgattta ttttacatgt
    78661 caatatgcac atgtaaaacg tggaaacttt actgtgatat aataaagaaa ataagacgag
    78721 aggtaggatt tttatgaata ctttagccta tatattatta tgcgtgcttt tatccaaacc
    78781 acgttctggg tatgaattga agcagctgat cacaattttt tgggaagccc atcacagtca
    78841 gatctataca acgcttgcca agttagacca gcagggttat gtgaccgttt tggacaaaga
    78901 cgagcactct caaaaaaaga tctaccactt gactgatgaa ggacgtgctc ttgtagagga
    78961 atggatcaaa gaggaaactc ccgctcctac gcaaaaagat gaatttctag caaaaatcta
    79021 tgctttttcg acgctcgata aaacaacagc tactggactg ctattcacta gagaacaaca
    79081 gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa
    79141 acaagaattt ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc agcaggaact
    79201 ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt tttgagtaag ggataaacag
    79261 aacctttcta gaaatatcta ttgaagagca aattatcttc aataaaagag caatacagtt
    79321 ggaaaagaaa aagcgatccg aacgacaggc caaaacctat cgttcggatc actatttttc
    79381 tagaactgca ttcaagagac tatgacaaaa agcatgccaa ggcctttttt caaataacag
    79441 tgttccatca ttcattactt tgaattgctt ttagcacact tcttgaaagc gggccaacga
    79501 tgatcaactg caacggtaaa gcggcaatca cattcatttt ccaagcattc atataagcag
    79561 agaataattg atcaggtatt ccttgttcca tgatcacacc gaataaagac atacatgtca
    79621 ccattcctaa aaccatcaag catgaaatag ttaagatcat ttggaccttg ctttctggat
    79681 ttactggtag tttaaaggca atttttttcg ccagcacacc gatgatgaag acatccaaaa
    79741 tgaaagccac cacgaatcca ggcactagac caatcaacaa acttgataaa tgaagatctt
    79801 gatgcagcca taaattatac gcgctcatcc ccaagaccat caaaaaacac atcattgttg
    79861 taaaaattat tccttctttt ttatttgtag gcaatctgta ccactccttt aaatttgtca
    79921 cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta ctttttttat tttctgccct
    79981 tttatttcat aaacttcatc agccgtacgc tcgacaaagt aagaatcatg agaagtaaac
    80041 aaaatcgttc ccggatatat ttgcattagc ttctctaaag cttcgatcgt tgccatatca
    80101 ataaaattag tcggttcatc taacagcaac acattacttg gcttagtaaa aagcaaagca
    80161 atcgtcagac gggtcgcttc tcccccactt aatgtacata acggtttcgt caaagtttcc
    80221 tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg attctgaaaa ctctgtttgt
    80281 ctcatgacaa aacgaatgat ggattcttca gatgtcattt gataaccctt ttgctgatat
    80341 acttgaaagc ttactttcgg agagagaagg attccttctc cttgtttgcg gatatgttct
    80401 aataaggttg tcttacctga accattttca ccgacaatcg cgattttttt acctaaagaa
    80461 aactgaaaat caccatttac cagtaatgtt ctcccacttc tttccaattg gacattttgt
    80521 cccatgatgg gatagcggct gtggagttca agagattttg gtactggaaa acggatttgt
    80581 ttcatctgct gcggcttggt tgtttctcct attttttgga gtcttctctc taatgttttc
    80641 gcattctttt ggatggcttt ttgtacactg tctttttgtt tagaggaaga caaccgatcc
    80701 agtctgattt gttgcttttt ctttttactg gacacttttt ccaacttttc cgcttgtttt
    80761 ttcttttctt ggatcgattt ttccaatcgt tttttctctt tcatgatcga ctctgcttct
    80821 cgtagctgag tctttttctc caattccttt tgagtgagat aggcactata attccccgaa
    80881 aactctcgga tttctccgtc ttttacttcc cagattttcg atgctagctc atctagaaaa
    80941 tagcgatcat gactaacaaa aaccagcgta ccataataat aacgaagctc ttcaatcagt
    81001 cgatcaatac tttttctatc tagatgggtt gttggctcgt ctagaagcat cccttgttca
    81061 taagaagaaa gaagacgtat caactcgatc tttcgttctt cccctccgct ccatttccgc
    81121 tctgaagttt cttccagtct taactggaaa tgactggcta attccaaatt taggtccttt
    81181 gcttcagcga gataggttaa ttgaggcagg tagttgaagg tgatctctgt ttggattttc
    81241 ccatgatcgg gaaaaagttc accggctatt agtttgagca aagtcgattt accgacacca
    81301 ttttctccta tgatccctat acgatcttgc tgatagacac gaagagaatc aatagatagt
    81361 tctagctggt tgccaaagct gacttgtaga tttgttatat ttactgctaa attttccata
    81421 tgttttacac tcctatttca tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa
    81481 atctgtttgc aaaaaaactg catcacatct tttcctactc tgaacgaaat aactatgact
    81541 aaatcaccaa atctgtcata gctttcacct attctactgt cgttcgaaaa aatggacata
    81601 ctccatccat gaaattgttg tttatacatg tcttttgaat ttatttagtt gttattcaaa
    81661 agttcgaaac gtaatttcat cgatgcagtc atttttccga cctccttttt tattaggtta
    81721 ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc ttttattcga
    81781 acatagtgat cttttctggt gctaaacgaa cagactcgta accttcttct accgcttttc
    81841 ctattgataa aataataact ggaacataac ggtctttatc caaaccaaat gcctctgcca
    81901 actgatctgc ttcgaatcca ccaattggat ttgtttcata gccatgatca cgcgcaacta
    81961 acatgaattg catagccgct aagctagaat cgactttgac tacatcgttc atttcttctc
    82021 ttgaaaaact cttgtaataa ggaatgatcg cagctaattg ttgatctctg acttcttttg
    82081 gcatcttccc tgattcgtaa gcttggttgt agatttcttc gccatattcg taacattgca
    82141 tatcgccaaa aatcatgacc attgcagaag aagtatcatt ttgtctcgta ttgaaacgga
    82201 tcaaaggacg caagatttct tttgcttcct cgctttcagc gaccacaaag cgccatggtt
    82261 tcatattgac agaagaaggt gcagtcgtcg cttctttgat catttccagc atttcttcgt
    82321 gtgggatttt gtatgtttca tcatatacgc gaacggatct tctaccagtc ataattgttg
    82381 aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt tatattgcaa
    82441 agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac atcgctgaaa
    82501 gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctt gattttcttc
    82561 tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt tctctgacgc
    82621 gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc ttgatcaatc
    82681 tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag tctcatcatg
    82741 atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac gattttttga
    82801 tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat aatttcctcc
    82861 tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa
    82921 gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga
    82981 aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa aaccaagatt
    83041 attgattctt atcaaactat agaagatagc tcttctgcag tagataattt tacaccgcca
    83101 aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt ggatcagtat
    83161 gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt tgagtaaacg
    83221 gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg
    83281 tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac ctctttttaa
    83341 taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca atctctttcc
    83401 ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa tagtcgataa
    83461 attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc gatttttgtt
    83521 aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta aggattgtat
    83581 aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc atattcccag
    83641 cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg ttctcctgta
    83701 tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc atactgtgtc
    83761 agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc agtggcataa
    83821 cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt ccatgcaccg
    83881 gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc
    83941 aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat ccattgccca
    84001 ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc tttaatccca
    84061 aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact ttcaacgatg
    84121 gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact ttgtgcagtt
    84181 gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc ataaaaagca
    84241 tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc
    84301 ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg agcttctgct
    84361 gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc ttcggttgaa
    84421 attgtatttt ctgagacagt agctgactgc aaagtacttt ctgtatcgtt ttcaattcct
    84481 gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc agctagcact
    84541 gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa attctttgct
    84601 ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt aactaaaaaa
    84661 gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttaat aaatttgctt
    84721 tttctgcata aaaagacgta aatgaagaat attcttctcc attcacgcct taaattgatg
    84781 gtcgtttgaa taactattta acaagtaatt ctctctggat attatccgtt atggattata
    84841 cttcctgtgt cgtcggacgg ataatcatgt tgctgacaga catccggtct ggcgtttcta
    84901 tcgcaaatgc tacagcagaa gctatatctt cagatgttaa tccccattct ttttgcgctt
    84961 catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca gtttgaacag
    85021 ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga cgtaatcctt
    85081 ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca ggataaacaa
    85141 catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt tccatgattg
    85201 gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc atattttgcc
    85261 attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca ttattgtaca
    85321 atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa
    85381 aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat tcttttattg
    85441 cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt tctgctaata
    85501 gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc actttttctg
    85561 ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat actcactatc
    85621 tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt ttggcaggga
    85681 ttcctgcaac gatggtattg tctgggacat ctttcgtaac agttgcattc gctgcaacaa
    85741 tggcattctc gccaatcgtt acgcctggca gaatagttgc tcctgctccg atccaagcat
    85801 ttttcttgat gtggattggc tttactgaca agcctcttcg ttcttttgga tcggttagat
    85861 ggttcaccgt gatgaggttg acacgaggac caatcaatac tgaatcttct aaacagatgc
    85921 cacctaagtc aacgaacatc acttgacgat tgataaagac atttttccct atactaatat
    85981 gtttgccgaa atctgtataa aaaggcagca gaacagtcac cgtatcatct actttgtcac
    86041 taatgatatt ttctagccgt ttgcggatat ctgctggttc atggtaccca gtattcagcg
    86101 ctgctaaagg ctcttcgttt tccgcttgga tctgatggat cacttcaaag agcggatccg
    86161 ttactcgaat ctctttattg acgatttgtt tttcgatgag gcttgtcatt ttgctccctc
    86221 cttttatcct tactttataa cggacggctt atgatgtcta atacttataa aacatactta
    86281 actattcctt ttaactatac ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg
    86341 atattcgtat actgacgtat tttttagcag tagctagaga aaaaaatatc agccgagcag
    86401 caaaatctct gcatatcacg caacctactc tctcaaaaca actgaaagat ttagaagaag
    86461 aacttggtgt gacacttttc actcgaggct cccgagagat acagttgacg gattctggtc
    86521 gatatcttta ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt
    86581 taacaaatga cgcaatcatc tctggagaaa tctacattgg cggaggcgaa actaaagcaa
    86641 tctctttcat ttctgatagc cttcattcgt tgatcaaggc gcatcctgat atccagtttc
    86701 atttatatag cgggaatgca gatgatatca tagacaaact ggatagcgga ttacttgatt
    86761 ttggactcgt aatcgaaccg acaaacaaac aaaaatacca gtcatttcga ctgccagccc
    86821 aagatcgttg gggacttttg atacatcgga atcatccttt agcaaagcaa acagtcatcc
    86881 atccgcagga actgaaaact gttcctttga tcatttcccg gcaaacgtca gtggacagtc
    86941 aactagctga atggctaggt ttcagcatag atcagttatc gatcgtcgga acatataact
    87001 tattgtacaa tgcttctttg atggtcgata acgggacttt tggtgcatta gctctagatg
    87061 ggatcatcaa tacttatggg acggaaaaag tattcatccc actcgatccg ccattacaag
    87121 cttcactgaa tctcatttgg aaaaaagacc agcctctttc tcctgctgct cgtaaatttt
    87181 tagatgtcct aacggagaaa tacagttaag tagagttttt ttccttcgct ttcagctttt
    87241 taacaacgaa taagttaagc aaaagatcca atgattaaat aacataagtt tcttaatttg
    87301 ttccttcctt ttatttctga taattccagt aaattactat aattataaaa aaagaattga
    87361 caaaacataa ctgtctatat aaaattgatt acgttaacaa taattcgttt catgtgacta
    87421 gatcatacta ggcatgaaaa atctctgagg aacatggttt ccctaggagg tttttcgtgt
    87481 ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt gaaaaccaaa tcttcaaaaa
    87541 gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat gcagtattgg tagatgacgg
    87601 tggacaggaa aaagtagcta ttggaaaagg gattggattt gacagaaaaa gaaatgattt
    87661 gatttttgat cgtgatgtcg aacggatatt tgtcatggaa ccagaaggtc aaatcaagtt
    87721 gcaaaattta cttaatcaga ttgatgaacg ctttttattt gcagcagaaa cgatcattga
    87781 tcatgcggaa actgttttga tggaaaagtt gaatgaacat gtattgatcg ctttgaccga
    87841 ccacattgct ttttctgcgg agaacattaa taacggtatc gtcatccgca acaaactttt
    87901 aagagaaatc gaagtgcttt acagtgatga attcagtatt gctcaatggg cagtagagta
    87961 tctgacaaaa gagcttggga ttccatatac ttatgacgaa gcaggatata tcgcaatcca
    88021 tattcacagc ggacgaagcg gtcggaacaa taaccatcgc agtatccgtg aagtgacgat
    88081 tgtatctgac atcatccatt tagtggaatc agaattggaa acagatatcc attcagaacc
    88141 attttcactg aattattcaa gactggtgaa tcatttacga ctgcttctac aacgaaccca
    88201 tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa atggtcaaac gaaaataccc
    88261 agaaagctat aagatttcta aaaaaatccg tgtgttgctg accaaagaat accagctcgc
    88321 gatcacgaaa gaagaactgg gttacttggc catccatatc gagcgtttaa gaagcgcaat
    88381 cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat tgaaagcata tctacaacga
    88441 atgggacgtt cccttcaact tccagttgcg gtcttacctg ctgctgcttt actagtaggg
    88501 atcggaaact ggtgggcatc ttatagtaac gacattgtcg ctcatttttt acaagctggc
    88561 ggtaacgcag ttttaggtca attacctttg ctgtttgccg ttgggttagc actagggatg
    88621 tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg tcgcatttga agttccagta
    88681 aatgttttaa aaactgattc agtagctact ttattgaata tcaaaccaga atcagttgat
    88741 ccatcttttg ctcagatcag taacgtattt attggtatta ttgccggatt gattgcggca
    88801 gctctttata atcgtttcca cgaagcaaaa ttaccgatgg ctttatcttt ctttagcggc
    88861 aagcgtctag tgccaatctt ggctgccttt gtcatgttgc tgatcagtgc agtgctactg
    88921 ctcgtatggc ctccggttta ctctgcttta gtttcttttg ctaaattcat tttgagttta
    88981 ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt tactgatccc tacaggattg
    89041 catcaagcaa tgaactccgt gttctggttt gacgtagcag gaatcaatga tattggtaaa
    89101 tttttggctt ctgaaggaac aaaaggcgta acaggaatgt atcaagctgg atttttccca
    89161 atcatgatgt tcgggcttcc agcaggagca tatgctattt acagaaatgc acgtccagaa
    89221 aacaaagcaa aaacagcttc cttgatgtta gcagcagctt ttgcttcatt ctttactggt
    89281 gtaacagaac cacttgaatt ctcatttatg ttcgttgctt ggccattata tgtcttgcat
    89341 gcaattttca ctgggatttc tttagcagtg agtgcattct tccattggac agcaggattt
    89401 gcctttagtg ccggatttgt cgattacttc ctatctttga aaaatccgat tgcgaatcag
    89461 ccattgatgt tgatcgtcca aggattagta acagcagtga tttactactt tggctttaac
    89521 tttgctatca aaaaattcca tctgatgact cctggccgcg aggaagcaga tctttcagat
    89581 gacgatactg caacgactaa ttcagacaat aaatatgcgg cacaagcaag caaaatctac
    89641 gctgcattag gcggagccga caatgtaact tcgatcgata actgtactac acgccttcgt
    89701 ttgcaagtca aagatacagg cctgatcgat caaaataaaa tcaaagccac tggcgtacct
    89761 ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg taggtacaga agtacaattc
    89821 gttgcagatg agatggcaaa actacatggt ggtgctgcaa cacgacctgc acaaacaaac
    89881 accgtggtta aaacagaaac atttgccggt ggaacgatcg aacaagatat ttacgctatc
    89941 gcaaatggga aactgattcc aatcactgaa gtttcagatg atgtattcgc ggaaaaaatg
    90001 atgggtgacg gctatgctgt attaccagaa aatggagaaa tcttctctcc aatcgctggt
    90061 acgatcacaa acattttccc aacgaaacat gctgtcggaa tccaaacaga tgctggtatc
    90121 gaagtcttgc ttcatatggg catcaacact gttgacttaa aaggcgaacc attcacttta
    90181 tatgtaaaag aaggtcaaaa agtcgcaaga ggtcaattga ttgctttagt tgatcttgcc
    90241 gctattcaat ctgcaggcaa aaacacggat atggtcgttg tattcacaaa tggcgacaaa
    90301 gtccaaagct tagaaatcga acccgcacgt gatgtaaaag caaatgataa aatcggctct
    90361 gtttccaaca aagcctgagc catcgtcttc cccagacttt tttatcgata aacaaacagg
    90421 agctgagaca aaaatcttgt ctcagctcct ttttccgaat aaaaacggcg ttctcctgcc
    90481 aactccgcgg taataagtcc agccaaacgt aacatatgaa gagctatttt ttatttgact
    90541 cttcttatta cttgatgcag aacagctttt ttacaacctc tgataaacgg tgttcaaaaa
    90601 ccagtttatc ggtatgaagt caaatttatg caaaaatgtg gaaagccatt ttcacaaaat
    90661 ttttcttcat actcaaaact aatcggcttt ttcacaacct ctttatacat tattcctgat
    90721 ccaatatttc ttttaatgct gcttcaagag aagttaatgg ttttcccaat actttttcta
    90781 aatcatcaga ggaaatagct aactgatcct ttttaatatc atgttggatc gctaaaaaca
    90841 tgtcagctgc ttcttgaggg aatccggctt cttttagatt ttcgacaaac gctgcatcac
    90901 ttgcttcaat aatttctaac tttttacctg tcgcacgctc taatgccttg gctaattctt
    90961 catagtcagt tagattgccg gataattcta agatttctgg agagtcactt tctactagta
    91021 cttttgctgc agcttctgca tattcacgtt tcaatgccca acctgttttt ccatttcctg
    91081 cagcataaac gaattgacca gtttttaaag cctgcccaat gataggtaat tcattctcta
    91141 cataccaatt attccgtaaa aatgtatggg aaatacctga atccttaatc attttttcag
    91201 tgaattggtg atctggcgct aatacactga tcgagtgatc ggcatctgca aaacttgtat
    91261 aagcgatata agatacccca gctgcttgtg ctgccttcac tacattctta tgttcggctt
    91321 gacgatttcc tggaacgcca gagacaaaaa gcaatcgatc tattcctttc aacgcttctt
    91381 tcattgagtc taaatcatca taatcaccaa tacgtatatt aaatccagct tctttcagtg
    91441 cttctccttt ttcttcacta cgtactagta catagatttc attttgtgga accatctttt
    91501 ttaagaattc cagcgcataa ccaccaaaac ctccagttgc tcctgttact aaataatgca
    91561 tatttgccac tccctattct ttttatttgt taagatttac agctgtacat ttataaatta
    91621 cacctttatc ataaagagtg tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa
    91681 ctgatgaagt attctctgca atttagtgat gcaatccatg tacttgccta tattgaaatg
    91741 tttcaagata cccgatcact ttctagtgag atgattgcca gtagtatcga aacaaatgca
    91801 gcaaatgtac gtaaaatcat gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc
    91861 ggaaaaccgc agccttccct tgccaaaagt ccagatcaga tttctttact agatgtctat
    91921 cgcagcatcg aaggtaatac acagcttatc caagtcgatc caaaaacaaa tccaaattgc
    91981 gtcgttggtg cgaatatcca agaagcttta gaagaaagct acgctcggct gcagaaaaag
    92041 gcagaagaag aaatggctaa gatcacactt gatagtctga tacataaaat agccgttttg
    92101 gaaaaagaaa aaagaccaga aaacacaggc ctcgtagaga gatttttata attctctcta
    92161 ctgtgcctgc atttttctga tcggaatcgt ctacataaat tcttttattc tccgggggac
    92221 aaaagccttg tcatgcccct ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg
    92281 acgtcttgat aaacggtgtt caagtagctg accctcggca ttagttctga aaaatgcaaa
    92341 atatggaaag ctatttccca tttttcctct ctaatgctca ggtcaaagcg ctactttcac
    92401 gacctctata ttatcatggt ttattgacta ccggtatcca gatttctccc atatatttcc
    92461 cgttggtaac tcccatttcg actacagcat ttggaccccc aacataggcg taatcttcta
    92521 cttctcttaa aacatgccca aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg
    92581 attctgctaa agcgattaca acaagatacg ttccttttgg gaatgggatg attcgggtag
    92641 tgtttggaac atcctgactc gtttgaacac cgacatagta catcattttc ccgttatagg
    92701 gttcgttgac tgtaaagaaa taatgatttt cagcagcttg tttcaatgca tctattgttc
    92761 cattaccgat aactttttgc tcaaatgctt gtttctctgc agccaaaccg acaaaatccg
    92821 tattgctact cttcaattcc acaccaatac ccgtcaccgt aaaatctggt ttttcttcga
    92881 tcctatactt tgccatttct cccacccttt ctattatatt aagttcatct tacataaaat
    92941 taaagggaaa tgacacgatt acgctctttt ttctagggat atttttattt tttaactatt
    93001 tttttgcttt cactgcaatc caaatctcac tgtaaacatt actgaaatcc tctccccatt
    93061 ttgtaaaaga gatctcggga gcttcgacta actcataatc tgaagaagga agccattctg
    93121 catagatttt cccccaagta tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa
    93181 tggcccaagt gtgttcttct atagcaagca tttctaaatc agcgtacgga ttttcttttg
    93241 tcgtagcaaa gccaatcatg tgcatcaaac tgcccttttc ttccaagcgg gctccatcaa
    93301 atccataaga aacgttgatt acttgatttg gatataaatc tcctaactta tgcatctctt
    93361 ccctttgagt ctccgtgatc gtctgcgcta acttttgtat ttcttgattg actccttcaa
    93421 atttgacagg aacttttttc gccacacctg ccacataaaa ctgttctttc ttttcgatac
    93481 gaaattccat cgattttcct cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt
    93541 tttgtatgcc gtatctgctg atttctgaag gcagatatcc tgaccaatca cgaaaagctc
    93601 ttgaaaaacc ttcgactgtt tcatatccat atctaaacgc cgtttctgtc actcttgatc
    93661 catctaggag atcgcgatta gccaaagcca atctacggtt tttgatatag tccattagcg
    93721 acatccctgc aataaacgta aagatgcgtt ttaattgata ttcagagatt cctgtatttt
    93781 cggctattat tttataggaa agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt
    93841 ggttcagatc tttaagcata tttatcttcc tccttgcttg cctttttatc atattatcaa
    93901 agaaagtcaa ttacttgatg aatcaggaac caatttagtc agatgtagaa aatgttgtat
    93961 ggatctaaag aaaattctca agcagcgttt cgtaacggat aattggtatt atctgcctat
    94021 ctatgtttta cctttagaaa aataaatttt ggggaagaca cattctaagt aaacaaagcg
    94081 gtaaaaatat cttttattcc cttagttaat ccgcttacta cctactgtac aaagcaaatt
    94141 gttgttcaaa ctgctgatca gaacgctgct tggatttttc tttgcgatac atcatacctc
    94201 ttttatcaat tggaacaatt atttatttct aacaaaatca cttcattttt gtcagataaa
    94261 taaa
//
LOCUS       DACDTH010000004        84310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDTH010000004 DACDTH010000000
VERSION     DACDTH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.004"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            281..973
                     /locus_tag="IWK54_000294"
     CDS             281..973
                     /locus_tag="IWK54_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9592448.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1181..1471
                     /locus_tag="IWK54_000295"
     CDS             1181..1471
                     /locus_tag="IWK54_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592449.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            2185..3555
                     /locus_tag="IWK54_000296"
     CDS             2185..3555
                     /locus_tag="IWK54_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592450.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(3795..3878)
                     /locus_tag="IWK54_000297"
     tRNA            complement(3795..3878)
                     /locus_tag="IWK54_000297"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3842..3844),aa:Leu,seq:gag)
     gene            4261..5478
                     /locus_tag="IWK54_000298"
     CDS             4261..5478
                     /locus_tag="IWK54_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9592451.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            5662..6498
                     /locus_tag="IWK54_000299"
     CDS             5662..6498
                     /locus_tag="IWK54_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9592452.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            6574..7722
                     /gene="nagA"
                     /locus_tag="IWK54_000300"
     CDS             6574..7722
                     /gene="nagA"
                     /locus_tag="IWK54_000300"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9592453.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            7942..9000
                     /locus_tag="IWK54_000301"
     CDS             7942..9000
                     /locus_tag="IWK54_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9592454.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            9019..10038
                     /locus_tag="IWK54_000302"
     CDS             9019..10038
                     /locus_tag="IWK54_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9592455.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(10140..10856)
                     /locus_tag="IWK54_000303"
     CDS             complement(10140..10856)
                     /locus_tag="IWK54_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9592456.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(10825..11610)
                     /locus_tag="IWK54_000304"
     CDS             complement(10825..11610)
                     /locus_tag="IWK54_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9592457.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(11601..12350)
                     /locus_tag="IWK54_000305"
     CDS             complement(11601..12350)
                     /locus_tag="IWK54_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9592458.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            12648..13172
                     /locus_tag="IWK54_000306"
     CDS             12648..13172
                     /locus_tag="IWK54_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592459.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            13188..14687
                     /locus_tag="IWK54_000307"
     CDS             13188..14687
                     /locus_tag="IWK54_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9592460.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(14775..15377)
                     /locus_tag="IWK54_000308"
     CDS             complement(14775..15377)
                     /locus_tag="IWK54_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9592461.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            15481..16437
                     /locus_tag="IWK54_000309"
     CDS             15481..16437
                     /locus_tag="IWK54_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9592462.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(16742..17698)
                     /locus_tag="IWK54_000310"
     CDS             complement(16742..17698)
                     /locus_tag="IWK54_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9592463.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            17872..18432
                     /locus_tag="IWK54_000311"
     CDS             17872..18432
                     /locus_tag="IWK54_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592464.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            18425..19249
                     /locus_tag="IWK54_000312"
     CDS             18425..19249
                     /locus_tag="IWK54_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9592465.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            19259..19876
                     /locus_tag="IWK54_000313"
     CDS             19259..19876
                     /locus_tag="IWK54_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9592466.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            19894..20577
                     /locus_tag="IWK54_000314"
     CDS             19894..20577
                     /locus_tag="IWK54_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9592467.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            21260..22495
                     /locus_tag="IWK54_000315"
                     /pseudo
     CDS             21260..22495
                     /locus_tag="IWK54_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            22503..23390
                     /locus_tag="IWK54_000316"
     CDS             22503..23390
                     /locus_tag="IWK54_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9592468.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            23407..24243
                     /locus_tag="IWK54_000317"
     CDS             23407..24243
                     /locus_tag="IWK54_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9592469.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            24246..24869
                     /locus_tag="IWK54_000318"
     CDS             24246..24869
                     /locus_tag="IWK54_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592470.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            24866..26158
                     /locus_tag="IWK54_000319"
     CDS             24866..26158
                     /locus_tag="IWK54_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9592471.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      26517..26614
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            26730..27311
                     /locus_tag="IWK54_000320"
     CDS             26730..27311
                     /locus_tag="IWK54_000320"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9592472.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            27314..28621
                     /locus_tag="IWK54_000321"
     CDS             27314..28621
                     /locus_tag="IWK54_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9592473.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            28914..29402
                     /gene="purE"
                     /locus_tag="IWK54_000322"
     CDS             28914..29402
                     /gene="purE"
                     /locus_tag="IWK54_000322"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9592474.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            29386..30519
                     /gene="purK"
                     /locus_tag="IWK54_000323"
     CDS             29386..30519
                     /gene="purK"
                     /locus_tag="IWK54_000323"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9592475.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            30532..31827
                     /gene="purB"
                     /locus_tag="IWK54_000324"
     CDS             30532..31827
                     /gene="purB"
                     /locus_tag="IWK54_000324"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9592476.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(32007..32384)
                     /locus_tag="IWK54_000325"
     CDS             complement(32007..32384)
                     /locus_tag="IWK54_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592477.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            32640..33368
                     /locus_tag="IWK54_000326"
     CDS             32640..33368
                     /locus_tag="IWK54_000326"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9592478.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            33368..33622
                     /gene="purS"
                     /locus_tag="IWK54_000327"
     CDS             33368..33622
                     /gene="purS"
                     /locus_tag="IWK54_000327"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9592479.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            33624..34295
                     /gene="purQ"
                     /locus_tag="IWK54_000328"
     CDS             33624..34295
                     /gene="purQ"
                     /locus_tag="IWK54_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9592480.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            34296..36518
                     /gene="purL"
                     /locus_tag="IWK54_000329"
     CDS             34296..36518
                     /gene="purL"
                     /locus_tag="IWK54_000329"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9592481.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            36503..37942
                     /locus_tag="IWK54_000330"
     CDS             36503..37942
                     /locus_tag="IWK54_000330"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9592482.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            37974..39017
                     /locus_tag="IWK54_000331"
     CDS             37974..39017
                     /locus_tag="IWK54_000331"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9592483.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            39014..39592
                     /gene="purN"
                     /locus_tag="IWK54_000332"
     CDS             39014..39592
                     /gene="purN"
                     /locus_tag="IWK54_000332"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9592484.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            39589..41124
                     /gene="purH"
                     /locus_tag="IWK54_000333"
     CDS             39589..41124
                     /gene="purH"
                     /locus_tag="IWK54_000333"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9592485.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            41140..42414
                     /gene="purD"
                     /locus_tag="IWK54_000334"
     CDS             41140..42414
                     /gene="purD"
                     /locus_tag="IWK54_000334"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9592486.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            42536..43450
                     /locus_tag="IWK54_000335"
     CDS             42536..43450
                     /locus_tag="IWK54_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9592487.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(43583..45148)
                     /locus_tag="IWK54_000336"
     CDS             complement(43583..45148)
                     /locus_tag="IWK54_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9592488.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(45141..46592)
                     /locus_tag="IWK54_000337"
     CDS             complement(45141..46592)
                     /locus_tag="IWK54_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9592489.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(46606..47622)
                     /locus_tag="IWK54_000338"
     CDS             complement(46606..47622)
                     /locus_tag="IWK54_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9592490.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            47842..48930
                     /locus_tag="IWK54_000339"
     CDS             47842..48930
                     /locus_tag="IWK54_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9592491.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            48984..50510
                     /locus_tag="IWK54_000340"
     CDS             48984..50510
                     /locus_tag="IWK54_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9592492.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            50512..51717
                     /locus_tag="IWK54_000341"
     CDS             50512..51717
                     /locus_tag="IWK54_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9592493.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            51974..52951
                     /gene="guaC"
                     /locus_tag="IWK54_000342"
     CDS             51974..52951
                     /gene="guaC"
                     /locus_tag="IWK54_000342"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9592494.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            53215..54639
                     /locus_tag="IWK54_000343"
     CDS             53215..54639
                     /locus_tag="IWK54_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9592495.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            55100..56209
                     /gene="pdhA"
                     /locus_tag="IWK54_000344"
     CDS             55100..56209
                     /gene="pdhA"
                     /locus_tag="IWK54_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9592496.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            56212..57189
                     /locus_tag="IWK54_000345"
     CDS             56212..57189
                     /locus_tag="IWK54_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9592497.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            57214..58857
                     /locus_tag="IWK54_000346"
     CDS             57214..58857
                     /locus_tag="IWK54_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP9592498.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            58865..60271
                     /gene="lpdA"
                     /locus_tag="IWK54_000347"
     CDS             58865..60271
                     /gene="lpdA"
                     /locus_tag="IWK54_000347"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9592499.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            60433..60708
                     /locus_tag="IWK54_000348"
     CDS             60433..60708
                     /locus_tag="IWK54_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9592500.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            60714..61481
                     /locus_tag="IWK54_000349"
     CDS             60714..61481
                     /locus_tag="IWK54_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9592501.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            61649..63478
                     /gene="typA"
                     /locus_tag="IWK54_000350"
     CDS             61649..63478
                     /gene="typA"
                     /locus_tag="IWK54_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9592502.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            63739..65511
                     /gene="recQ"
                     /locus_tag="IWK54_000351"
     CDS             63739..65511
                     /gene="recQ"
                     /locus_tag="IWK54_000351"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9592503.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            65625..65927
                     /locus_tag="IWK54_000352"
     CDS             65625..65927
                     /locus_tag="IWK54_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9592504.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            65930..67093
                     /locus_tag="IWK54_000353"
     CDS             65930..67093
                     /locus_tag="IWK54_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9592505.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            67119..70547
                     /locus_tag="IWK54_000354"
     CDS             67119..70547
                     /locus_tag="IWK54_000354"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9592506.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            70568..71692
                     /locus_tag="IWK54_000355"
     CDS             70568..71692
                     /locus_tag="IWK54_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592507.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            71682..72119
                     /locus_tag="IWK54_000356"
     CDS             71682..72119
                     /locus_tag="IWK54_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9592508.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            72103..72441
                     /locus_tag="IWK54_000357"
     CDS             72103..72441
                     /locus_tag="IWK54_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9592509.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            72560..73120
                     /gene="rsmD"
                     /locus_tag="IWK54_000358"
     CDS             72560..73120
                     /gene="rsmD"
                     /locus_tag="IWK54_000358"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9592510.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            73117..73608
                     /gene="coaD"
                     /locus_tag="IWK54_000359"
     CDS             73117..73608
                     /gene="coaD"
                     /locus_tag="IWK54_000359"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9592511.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            73598..74641
                     /locus_tag="IWK54_000360"
     CDS             73598..74641
                     /locus_tag="IWK54_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9592512.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            74800..75528
                     /locus_tag="IWK54_000361"
     CDS             74800..75528
                     /locus_tag="IWK54_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9592513.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            75644..75889
                     /locus_tag="IWK54_000362"
     CDS             75644..75889
                     /locus_tag="IWK54_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9592514.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            75886..76104
                     /locus_tag="IWK54_000363"
     CDS             75886..76104
                     /locus_tag="IWK54_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9592515.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            76240..76779
                     /locus_tag="IWK54_000364"
     CDS             76240..76779
                     /locus_tag="IWK54_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9592516.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            76772..79045
                     /locus_tag="IWK54_000365"
     CDS             76772..79045
                     /locus_tag="IWK54_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9592517.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            79083..80129
                     /gene="holA"
                     /locus_tag="IWK54_000366"
     CDS             79083..80129
                     /gene="holA"
                     /locus_tag="IWK54_000366"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9592518.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(80218..80472)
                     /locus_tag="IWK54_000367"
     CDS             complement(80218..80472)
                     /locus_tag="IWK54_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9592519.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(80872..81141)
                     /locus_tag="IWK54_000368"
     CDS             complement(80872..81141)
                     /locus_tag="IWK54_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592520.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(81611..82066)
                     /locus_tag="IWK54_000369"
     CDS             complement(81611..82066)
                     /locus_tag="IWK54_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9592521.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(82204..84132)
                     /locus_tag="IWK54_000370"
     CDS             complement(82204..84132)
                     /locus_tag="IWK54_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9592522.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        27483 a        14397 c        18141 g        24289 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacacat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgcttaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
     1021 tatactgctg cctctttttt ttagtacatg tccttcatct tcgcaggaag tgccaatatg
     1081 attttaagaa aggtaataga caaaaagtca aaaagataaa acagagtttc ttctttacaa
     1141 agggaatgta tgttcgtata atggtattga ggtgaaggtt atggagaaaa aatatggaac
     1201 agtagataag atccgtactt tgcaagttag tgccagaccg ttgatccgtt tcagtttaaa
     1261 tggggtcaat tgtttgattg cttcccatag tttgaacttc ctagctgaag tagacgaagg
     1321 gatgaagtta gtcgttacag gttattataa taatagaaag caatttgttg tgagatccta
     1381 tcatgtgtta gggaaaccca aaattgtggt agaatatgag aaaagtctat acccaagaaa
     1441 aaaggtccga caagatgaaa gaataaacta gaaaacaagc ggctgagaca aaaatgtcct
     1501 agccgcttgt tttggaaaga tggatgcaga cgatgggatt tgaacccaca cgagcatacg
     1561 ctcacaagaa cctcaatcta tatataacta catgtattta agtatttgtg atgaatcact
     1621 aaactttcca aactcatttg agggatattg cttaaagatt gaacagatca attcgaagcc
     1681 ctattagtat tcgctcaatg gggggatcag aggccataaa aagagactag tcattactag
     1741 tctcaggtag tggaattgta ggattttaaa cgttcctgga gagtaccggt atgtaactcg
     1801 aagaggtgat tatcatagtc gtaaaagtaa attgaagaag cctcgccttt cacacgagaa
     1861 cgtcctggtt cgacagttaa acctaacatt tggatttttg agacaaacga atctatatct
     1921 tgttcgtcaa ttttaaaagc aatatgattg tatgtctttg gcagtttatt cgacgaattc
     1981 tgcattactg caatccatag gtccttaact aagaaaaatt tttctggata taatgaaaat
     2041 ttttttgttg actatcgtag atttcttttg catcaaataa ggtatttaac aattaagtat
     2101 ttaaaagata acttttaagt tatctttaag taatgcttaa agctatgtta tactctatta
     2161 ggaaatataa gaaggagtat aaaaatgaac aaacataata gaaaaataac gaataacaaa
     2221 gaactgatct tgttactaat agggatttgg ggtatctttt tactctttaa tatgtatatg
     2281 ccgactatga gagcggatga cgtagtttat gcaagtagat tagatgaact tggctatttg
     2341 ggtgcttcga tagaacatta taagacatgg agttcaagaa ttattataga actattctta
     2401 atgttctttt ctaaacactt tatgctatgg aaattattaa attctacgat tatgttagga
     2461 attgtagtat tactttgcaa gtatgttttt gagaaaataa atgttaaaaa tttattattg
     2521 gtttgctcta tatactgttt aataccattg acagtaatgg gggaaaccgg ttggagagca
     2581 accaccttga actatcagtg gccagtagca tttagtttac taacttttta tccgtttttt
     2641 caactcttaa gaggagaaga aataaataga aaaatatatt gggtaagtat tcctttattg
     2701 atatttttaa ctaatcaaga acaagtcaac gcctgttttt ttgttctaac aagtatagtt
     2761 agtttatatt tgattgtcaa tggacgatat aattataagt tatcagtatt ttcaataata
     2821 agcttagcag aattaatttt ctctttgaca actccaggga atgcattacg tgctgcacat
     2881 gaaattaata aatggtttcc tgaatataaa aatttcaact ttcttaataa gttagattta
     2941 ggtatttcat catttggaaa gccgtttttt ttagacatga atatcttatt tttattgcta
     3001 tttttcttaa ttttccttct aacatatagg aaatgtcaaa actattatgt gcgtatattg
     3061 actgcattac cgtttttctt aaacctgatt atttattttg gtaatacaat gggtcaaagc
     3121 tttacttacg tcaatggaaa taaaagggca atgatttgga gtagtagtaa cctgaataat
     3181 ctctttacag agttaggaac gaaactgtca cttttttatc caggtacttg gatagctact
     3241 ttagtagtct tggcattatt actatgttta attgttggaa tttatttaag ttttgacaat
     3301 aagaaaactt ctattttttt agttattttg atgataatgg gattttgttc aaggttaatt
     3361 atgggatttt ctcccacagt atgggcttcc ggaatgcgga cgtactatat actttatgtt
     3421 gttatcgcca tcttagtact aatggcagtt aaagagctaa tgaaaagcat gagtgtacaa
     3481 aaaaatgaat ttatgcaatt tggattaacc gtgcttggta tttgtacctt tataataacg
     3541 gtgataaaca gataatatac aaaaaatccc tcgctttagt attaggtgag ggattttact
     3601 cgtttttcga ttaattgttt taacacttgc aagtttctac gagtagcttg tttccgacct
     3661 tcatatactc acctcaggct gtttacatga ttgtacgttc tttttgtaaa ggatacagtg
     3721 atatgtatat aatggcataa agaaacgttc aaacaaaaaa actcgtgaag caaaattaac
     3781 ttcacgagtt gtagtgcaga tgatgggact cgaacccaca cgagctaacg ctcacatgac
     3841 cctcaatcat gcttgtctgc caattccaac acatctgcgt aataataaca cactaatgat
     3901 agcgaggttt tacatagttg tcaagctcaa aaatgggagg acttattaag atggagaaat
     3961 atgacggaga attttcagga ctgggaatga tcttaggtgt attgattgga cttgcattcg
     4021 gacgtttttt gcttggtttt atgctaggaa tcatttgtgg aattgcaatg gattgggcag
     4081 cgaatctatg gaatgactat catgacaatt aagttctcaa cgcttgtttg tgttttttta
     4141 atacttacgt ttttcctaaa taagtggcaa aattttctag gatggagtat gatattgaaa
     4201 tagtaaataa caaaaaattt ggaaagttct aactttttga aatgagggat tggctaagtg
     4261 atgagagaat ttgaaaaatc caacaaatta gaaggtgtca gttacgatgt tcgcggtccc
     4321 gttctagaag aagcggagcg gatgcaagaa gagggcatta gtattttaaa attgaacacg
     4381 ggaaatcccg caccttttgg atttgaagca ccaaacgaag tcattcgtga tttgatcatg
     4441 aatgcccgtg actcagaagg ctactcagat tccaagggga tcttttctgc tagaaaagca
     4501 attgagcaat attgccaact gaaacatttt ccgaatgtga cgatcaatga tatctacacc
     4561 ggcaacggag tgagcgaatt gatcacgatg tgtatgcagg ggttgctgga caatggagat
     4621 gaagtactgg ttccaatgcc ggactatccg ttgtggacgg cttctattgc attagctgga
     4681 ggcacacctg ttcattatat ttgcgatgaa gaggcagaat ggtatcctga tattgatgat
     4741 atcaaatcaa aaataacgag tcggacaaaa gctattgtta tcattaatcc taataatcct
     4801 actggggcac tttatccaaa agaattatta gaagaaatcg ttgaagtagc tcgacaaaat
     4861 aacctgatta tttattccga cgaaatctat gatcgcttag tgatggatgg tctagaacat
     4921 attccgattg ctacattagc gcctgatttg ttcgttgtta cgctgaacgg tctatccaaa
     4981 tctcatcgtg tagcaggatt tcgagtaggc tggatggtcc taagtggaga taagtcacac
     5041 gtcaaaggct atatagaagg gctgaatatg ttgtcctcca tgcgtttgtg ttccaatgta
     5101 ttgtctcagc aaattatcca aaccgcgcta ggcggttatc aaagtgtcga tgagctgctc
     5161 ctgccaggcg gaaggatcta tgaacaaaga gaatatatct ataatgcaat caatgatatt
     5221 cctggtcttt cagccgtaaa accaaaagct gctttttata tattcccaaa aattgataca
     5281 aagcgattca atattttgga tgatgaaaaa ttcgttttgg actttttaca tgaacatcat
     5341 attctacttg tacatggtgg tggatttaat tggaaccagc cagatcattt tcgaatcgtt
     5401 tatcttccta aaatggatga tttgaaacaa acagctaaga aaatgagaga attcctagca
     5461 acctatcgcc aaaaatgatt ttaaaaacca aatttttcag taggagggcc acagacaaac
     5521 acactgaggc tctctttcta gtggagtttt cttaaataac taaagtatat tatttagttg
     5581 caagtctttt cttgaagttt gcgacttcct aaagtaaaat acgaatagaa tgcgaatacg
     5641 catattttgg ggaggatact tatggaaacg ttaatcgagt cctttggaac gatcattaaa
     5701 gagattcgaa aagaacaaaa aatgacgcaa aaaatgcttt cgcatgatat ttgttcacaa
     5761 agtgtattaa gtcggatcga aaataatgag gaactgccga atgtattggt gatgatgcag
     5821 atttgtcaac ggctaggggt aacgattgat catgtgatga atctctctcg aaaaactact
     5881 cggacaaacg ataaattatt tgaactgttg gatagttgtt tccaaaaacg tgattatcgg
     5941 aagttagaac aagtactaaa atcttctgat atttctgaaa atctgtatca agcaactgac
     6001 tttcaacgct attattatta tctcggtgtt tgcgaattca cgttgcgtca aaatatctct
     6061 cagtcccttt attatttgaa agaagcatta agctactcta gccaaaaaga aagaatccat
     6121 gtttctgaca cagaaataca actgattagt tgtatcggaa aaatatatgg tgtggcagga
     6181 aaaacggctg aagcacgcta ctatttgagt cgaagcatcc aactgctgca tcaatcagct
     6241 gatgaacgat caaaaagtga attttcgcaa attttttata attatggaaa tttcttattc
     6301 catcaaaatg aaatcgacga agcgttagaa caggtgaatc aaggaatcta ttgggctcaa
     6361 gagaagaata gctactatta cctcaatgat ttatttgtgc ttaaaagctt aatatacaag
     6421 cgtaaagaag aaccagaaag agcagcattc tatgaaggct tagcacaagc tgtgaaacaa
     6481 atttcaaaaa atttgtaaat ggtgtagacc attcttgact tcttctctaa ataagagtac
     6541 tttagaggta gaaaaacaaa gggagagaat agaatgagaa cttttatcta tgctgacaaa
     6601 ttctttttag cgtcaggtgt caaacatgca ggctatcttg aaattaccga tggacttttt
     6661 ggtgaataca ccaaagaact acctgaggga gaagtcaaca tcattgacca aagcggcaaa
     6721 tggattgctc ctggtttggt agatacacat attcacggct atatgaatca tgatgtaatg
     6781 gataatgatg cagaaggcat caaagtgatg tctgaaggtc tgttatcttg tggagtgact
     6841 tcatttttac caacaacatt gacgtcaagc aaagaacgtt taaaagatgt agctgagaca
     6901 atcggcaaaa tgtatcagga agcaccagga gctaaaatca aaggaatcta ttttgaaggt
     6961 cctttcttca cagaagaaca caaaggggca caaaacccaa gttatttcgg ggatcctgat
     7021 ttggatactt tccatgaatg gcaagaagct tcaggtggtt taatcaaaaa aattgcttta
     7081 gcaccggaac gtcaaggtgt agaagagttt gtcaaacaag tgactgaaga aggagtagtt
     7141 gtttctctag gacacagtaa tgctacacta gaagaagcgc aaaaagctgt tgaagcaggc
     7201 gcaagtgtat ttgtccatgc tttcaatggt atgcgtggat tgaatcatcg cgaaccaggt
     7261 atggtgggtg cgttattatc tttagaacat gtcttttctg aattgatttg tgatggccat
     7321 catgtacatc caaatgctgc gggaatccta atggaaaaag caggacatga tcatgtcgca
     7381 ttaattacag actgtatgat ggccggtggg atgcctgatg gaaattacaa tttaggcgaa
     7441 tttccagtag tagtaaaaga aggaacagca cgtctagaat ctggcaactt agctggaagt
     7501 atattgaaat tgaaagaagc aattaaaaat gttgtagact ggggattagc aacaccagaa
     7561 caagcagtca tgatggctag tctgattcca gcaatcagct gtaaaatcga tgatacatgt
     7621 ggaaaaatcg ctcaaggacg cgatgcagac tttatcgtat tgaaccctga tatgacattg
     7681 gaagcaactt atctagatgg cgtggaacgt ttccacgcat aatataattt aagttaacct
     7741 taaaactaaa cttcttaagt ttggttttaa ggtttttttg tgttctagta aaaatcactt
     7801 tacataagtt tcaaaagttt ctttaatttc ctattgttca ataagtaaca atgtaaagat
     7861 aaaattaaaa atgttaatac gtattagcaa aataaaagct ttttttattg tgaataaatg
     7921 aacataatta ggaggagaaa gatgaactta tttgacattt ttcgatcgac tttgacagat
     7981 atgtcgattg tcagtgcaat cacttcaaca gtttttatta tattattagg gtttgtttgt
     8041 agaaaaaagg ggattttttc tgaatctgtt ggaaaaattc tatcgaaagt cgtcttaaca
     8101 gtggctttgc cagcactggc atttcgagct ttcatgcaag atatcaatac agaattactg
     8161 atgcaaggaa tcaatgtctt gatttgggga ttagttattt atattattct tattctagtt
     8221 tcaaaacctt ttttcttaag ctataaaggg gataagcaaa caacgttgcg tgtcctgacg
     8281 atctttggtt ctacgacctt tttcggaacg cctatcgtca gtgcgattta tggtcctgtc
     8341 ggcgtgatgt attcctcaat tttcaatatt ggctaccgaa tctttttgta ttcttatgga
     8401 tacataaaaa tgagtggctt gaagatggaa gctaaaaata tcaaagcgat gttcgtcaat
     8461 ccaatcatta tcgctacttt tgccggcctt ttcatttggc tgttccaagg gtatctgcca
     8521 caagtgtcag ttacaggtgc agaaggagag ataagccaag tagcgttttt gcgaattgac
     8581 cagactgcac cgtggctatt tcagccgatg gattatttag ctggtttaag ttcaccattg
     8641 gcgtggctag caatcggcgc aacattaggt gaagtaagct tcaaaaaagc tgcatctgaa
     8701 aaaacttcat ggtattacag tctagtcaaa gtggtatttg tacctgcatt gaatatcgtg
     8761 attctagcag ttttgacggc aacgaatatt ctaccagtca attttgaagc actggcaaca
     8821 atcgttatca tgatggctac accagctgca acagtggcag cagcctatgc tatcagtttt
     8881 gatagggagg cacttttagc ctccaatgca tccttactgt caactatttt tgctgtggta
     8941 ctgacacctg tatggatcgt tgtattacaa atgattcatg gcataggttt attttcataa
     9001 gaaatggagg aagaaagtat gagaatcgca tgttacggtg ttcgtccgaa cgagatagat
     9061 ttttttgaaa aattgaatat ttaccattat gatctcagtc tttatgaaga actgttgaca
     9121 catgacaata tcgtgtcagc aaaggaccat gatgctgtat tgttgagggg aaattgtata
     9181 gcagatgaga ccaatcttgc aaaaatgcag gaatatggga ttcgttatgt ttttacacgg
     9241 actgtgggag tcaatcatat cgatctacaa gcggcagctg attttggtat gacagtagcc
     9301 cgtgtaccat cgtattcgcc aaatgcgata gcagagttat ctttgacttt tgccatgatg
     9361 cttttgagaa atactgcgta tacgacgatc cgaacatctt tcaaagattt tcgagtggat
     9421 gagcaaatgt ttagtcgtga aatcagaaat tgcacagtag ggatcatagg aacaggcagg
     9481 atagggttga cggaagctgt tttgtttaag ggactaggag ccaaagtgat cggctacgat
     9541 attcatcaga cagaagctgc taaaaaagta ttgacgttcc actcgcttga agagttatta
     9601 gccaaaagcg atattgtcag tattcatgta cctcatatcc caggtgaaaa cgatcaaatg
     9661 atcaatgaag gatttttagc tcaaatgaaa aaaggtagca tcttgattaa tacagcccgt
     9721 ggagagcttc aagataatga agctatttta agagctttga aaagcaatca tttagctggt
     9781 tttgcaaccg atgtttttgc aaatgaaaca gaggtttttt tccgatcctt caagccatgg
     9841 gagacgattc cagaccctgc tatccagcaa ttagtagaaa tgtaccctag agttcttgta
     9901 accccgcatg taggttcaaa tacagatgag gcattatcaa atatgatttc gacaagtttc
     9961 gagaactttc gtgaaataat agaaactgga aaaacgaaaa atgaggtatc tcttcctaaa
    10021 gcccgtcaat taagataaga aaacatggat attcgtcaat aaaatgttta aaaatagtcc
    10081 aagtaaacag ttgttacttc tgtatacttg gactattttg tatgggataa ttcgatgact
    10141 caacagtaat ctgtgagctt tttccgccca ttctttgtca attcataaca ggttagcggc
    10201 tggccgattt ccaaatattt gattctttcg ttcaatatat cttgttctac taaaaaattc
    10261 aaatatttct tgacggatat acgtgacagt gagctttttt gagccagttc ttttgttgaa
    10321 aaaatagtat cttcttttag aataaatgca cttatttttt ttaaagtcag ttttgacaga
    10381 cctttaggca aatcgatgaa tgatggtatt tcgtgaatgg gagtggtgtg gttgaatact
    10441 tgatcgatca atttctgatc ggctttttca gtcgtttgga ttgtctgaga gaaagcaaga
    10501 tacttttcga tcgccagctg aaaacgttgc aaagtgaacg gtttgatcaa ataatctaag
    10561 gcaccgtagc ggatcacatt ctttatggtt tcgatatcat ctgcagcagt gatcaaaatc
    10621 actgggatat catttccttg ctgtctaagc caagataaaa aatggatacc actttcgcca
    10681 ggcatgtata catcaagcag cagcagatct gcgttttttt gtctgaaata tttttttcct
    10741 tcttctacat ttctgaaact ttcgattacg ttcatattag ggatgctttc cacaaaagta
    10801 cgattcaatt ctgcaaccat tggatcatct tcgattatca ggacatttgt catcattttg
    10861 atcttccttt tttggtaatt ctagatagac gattgtaccg ccatttgttt gtttggaaaa
    10921 ttctaaaaat ccaccgtggc gtttgacgat tgaatggatg agattcaatc caaacccacg
    10981 attgattccc ttcgtcgaaa aacctagcgt aaatattttt tctgagattt cttctgatat
    11041 cccgacaccg ccatcgcaga cggtcacgat gaatacttca gcttctgtgt catagtttaa
    11101 atgaatccgg acctgtccgt ttctttcatg tttttgtagc acggcttctt ttgcattatc
    11161 aagtaagttc cctaacactt gcaaaagtga atggatggag tcatccatct ccattttagg
    11221 gataaaagaa tcgctttcta acagcatttc aacagattgt tctttggctt cgtttatttt
    11281 gcctaataaa aaaccggcaa ttgcaggtac tttgatcaga cttgagactt ggccaatctc
    11341 ttttttataa ttttgattta gctgctggat aaaatgggtg acttcttcat acttcttcaa
    11401 atcaatcaaa ccagaaatga catgaagttt attcatgaat tcatgggttt gagctctcat
    11461 ggagtttata tattgtttcg tccccccaag ctcctccatt aatttcatta cttctgatcg
    11521 atttcgtaaa gttgcgactg ctcctaaaaa acggtcattg ctaaaaagag gtgtaacgct
    11581 agaaatagtt tctatcccat ttaaatacat ggattgagtc cgaacggtcg tttgatgctc
    11641 aatcgtctct ttaaacaaaa cgtcatatag attatcactg atttttttcc tacttcgaaa
    11701 tccaatagct gctctgtttg gtagaaaatg ttttttgcag gtgtattgat caagaggata
    11761 gttttatcag aagagatggc taagattcct tcatctactt cattttcgat aatcgttttt
    11821 tcacttaact gttctgctat ttcttgtggt tcaagtccaa gcagtacttt tttcattttt
    11881 tgggcaagaa aaacggcgcc tatgatgcct acgctaagtc caataatcaa tgaaaaaaca
    11941 atcatttttt gtccatcttt gacttgattg tcaatcgtgt tgtttgttaa gccaacacaa
    12001 actgcaccaa taatctcttg atcataataa atagggacga aatagcggaa gccgaaccct
    12061 aacattcctt ctttttcaga gaaaaattct tgtcctccaa tcactttttt cacatcttca
    12121 tgacttgaga aaggaagacc gatgtaactt gggtctgcgt gagaatatcg aatgccttca
    12181 gggtccatga caacgataaa atctaaattc gatgcaatca tgatttcatt tgttattcct
    12241 tgaatcgcat gtgtcgggtc accagtctgc agcttttttt ggatgagttt atcattggca
    12301 acgattttag caatgctaga gattttatct gacacactag atcggttcat cgaaagaata
    12361 aaattcctga tcaatatggc agaaacaatc aaagaaacga atgtggagaa acaaacgaca
    12421 aggatgatcg tgttctggag ctttaatggt tttttcttaa aaaaaggaaa cataggctac
    12481 ctcgaaagaa tttattgtga aaattttaac atgaaaacaa aaatttaaca aattgtcaac
    12541 gataaacaca accttcctat aaaaaacacc actgagtcgg taaaagaagc tatacagaat
    12601 tttcaaacat tggtatgata gacgtagaaa tggaaggagg cgtgtatatg atagggggaa
    12661 tcgcacttga gacgatttat tggtatacat tgctgatttg tgcaggattg gcagtcctat
    12721 tgattatttt tggagatgta ttcgattttg atggcccgct tgatccgatg cttattatcc
    12781 cttggttaac tttcacaagc ttatttggtt ttctaggtga gcgtttaaca gatcaatcat
    12841 ctttattttt atttattgct agtggaggca tatcgatagt gttggtattt ttgcttaatt
    12901 tctatatatt gatgccgatg aaacatgccg aatcaacgat gtcgtcttct gaaaaaactc
    12961 tggaaggaca agtagctgtt gtgatcacac cgatccctgt acgaggtatg ggagaaattc
    13021 agttgaaaag tgttaccggt tccattagtc gtccagcttg tttttacagc ccccaagaac
    13081 agacagctga gcggggaacg aaagtattga tcattgagat caaggaaaga atctgttatg
    13141 tcatgcctta tgaaagcatg ataaaagctt aaaggagcgt gtgttaaatg gaaataccag
    13201 ttgtttttat cgcgatcgca atcgcagctt tgattttact gatgttgctg atcgtctttg
    13261 tagcgaagta ccagacagca aaaccggatg aagcgttgat catcagtgga agttatttag
    13321 gaagcaaaaa tgtgcacatt gatgaaagca ataacaaaat caaaattgtg cgtggaggtg
    13381 gcgcattcgt cttgccagtt tttcaacgtt cgaatcggat cagtctatta tcgagtaaat
    13441 tggacgtctc cacccctgaa gtgtataccg aacaaggcgt accggtgatg tgtgatggta
    13501 cttcgatcat caaaatcggt tcatctgtag aagagattgc tacagcagca gaacagtttt
    13561 taggcaagac aagagaagaa ctggaaaatg aagcaagaga agtattagaa ggacacttgc
    13621 gctcgattct tggttctatg actgtagaag aaatctatca aaatcgcgat aagttcagtc
    13681 aaagcgtaca agaagtcgct tctgtagacc ttgcaaaaat ggggttgatc atcgtttctt
    13741 tcactatcaa agaggtcaga gacaaaaacg gttatcttga ctctttaggg aaaccaagaa
    13801 tcgcccaagt caaaagagat gcagatatag cagaagcgga agctctaaaa gaaacgcgta
    13861 tcaaaaaagc tcaagcagaa aaagaatcac aagcagctga attacagcga caaacagaga
    13921 tcgcagaatc attaaaagaa aaagaactga aattagcaac ctacaaacaa gaacaagatg
    13981 ttgctaaagc aaaagcagac caagcctaca atttagaaag tgcccgagca caacaacaag
    14041 taatcgaaca ggaaatgcag attaaaatcg tagaacgcca aaaacaaatc gaactggaag
    14101 aaaaagaaat cacacgtcgt gaaaagcaat atgattcgga agtgaagaaa aaagcagatg
    14161 ccgatcggta cgctaaagaa caagaagcac aagctcaaaa agtcaaagaa gtgactgaag
    14221 ccgaagcaga acgattccgt gtggaagcac tggcagaagc ggaagccaat aagacacgtt
    14281 tagctggaca agcagaagcc gaagcagcac tagcaaaagg aaaagcagaa gccgaagcaa
    14341 aacaaaagat cgccaatgcc ttcaaggaat atggcgaagc agctgtcttg agtatggtta
    14401 tcgatatgct accgcaattg atgcgcgaag cagcacagcc gctaggaaat atcgaaaaaa
    14461 tctctgttgt cgatacagga agcagttcag gagaaaccag tggagcgaac cgagtgacaa
    14521 attatgccac aaatttgctt tcttctactc aagaaacact aaaagcgaca acaggacttg
    14581 acttgaagga aatcgtagag aatttgtcga cacgtgggac tactagccaa gtaaatatgt
    14641 atgaagcgcc aaaagaggca gaacaaaact cgatgaagga agaataaagt agtacacttc
    14701 accgttcaat caaaaatgga ggaagacttt ttaaagttct tctctccatt tttttatttt
    14761 attgcaaaag aaaattagtc ctcgctattt ttcttttgtt tttggatctt agcttctatc
    14821 tcttgtaaat ttgttcttag caatcgttct ttcttttgga gttcgaccaa atcttctgat
    14881 agttcttcct tcttttctgt ttcaggagga cgaagaaaga aattgatacc gattgcaatg
    14941 aatgtcccta taaaagtatc tagtacacgt tggatagcat acaaagcgga ttcgccttga
    15001 gggatgctca acgcaatcag caacaacgta gctattgcgg aaatgatacc ggaattattg
    15061 ttaataccat ctgacacgac gatcacaata ataactagaa taggaagcaa gatcaattct
    15121 accaaaaaat cattgtgaaa atagcttttg acaaaaaaat aaatgatagc taatgtacca
    15181 cctaatgtat tgccgatgat cctagacttt ccaaaagaaa cggttgtagt caagtcctga
    15241 cgcaaagaaa aaacagcaga taatgcagca atcatcggga ttcctcgttg agtcaaatga
    15301 aaaaacaaga tacataaaaa aacagctaag gcacttttga tcgtacgcat acctagtcga
    15361 aaacggccaa attgcataga gattctcctt tttggtcttt ttactatcat acctaaactt
    15421 ttgtctgata gctagttttt cattattcaa gtataattaa aataaaaaag ggggaagtaa
    15481 atgcgtatag gaatgcgaac agtcaaaaca gtgatcagtg tcttcttttc gatggtcaca
    15541 gcgggtagct tatcgctgtt gtattggcca gctgcaggga tcattgcatt attaagtgta
    15601 ggaaatacca agcgctctac attgcgtacg ggcatgaatc gaatcgcagc ctttatatta
    15661 gctacggcaa cagcactgtt cagttttttt gctgttgggt atactatttt tggcttcagt
    15721 ctttttttat tcctgttcat cccgcttgcc tctcgcttca aattaaccga agggatagta
    15781 gtaaattcgg ttttggtcac tcattattta gtggaggaaa acatgtcttg gcagctgatc
    15841 ggcaatgaag cgggattaat ggcgattgga ttagtatttg ctttgcttgc aaatgtttac
    15901 atgccagatc gtacgaagca gctgaaggaa aaccaaatac agatcgaaaa agaatttcga
    15961 aatattttga gacaaatggc agaatttctt ttatcagaaa ataaagaaga tgtgcagatc
    16021 aaatgcgaac atttactgac attcattcgc gaaagccaag aagatgccag agaacatcaa
    16081 gaaaactact ggctcagaca gccgctatac tacgaaactt acttctctat gagaagagca
    16141 caggccaatg tgattaaaga catgctggaa aatctagaga gaatccagca accagcttat
    16201 tatggaaaac atatctatgg tttattgatt tatactgcag aaacattttc agagtcaaat
    16261 gatggcagac aaattttaac acgtattgaa gaagtttatg tattgtatcg gcaaatgcct
    16321 ctgccgacta gtcgccctga attcgaggat cgagcagaat tgttccagtt tttgcagtct
    16381 tttaaaagtt ttattgaaat caaggcagaa ttctcccaac aaaaaatcca aaaatagagg
    16441 ttgtgaattc agctaatatt caggtcaaaa agctgatttc acaacctctt ctttaaacgg
    16501 tgttctcctg tcaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgtt
    16561 ttttgaaatt gctccttgtg actcggagct gaacggtagg ctcacaacct ctttcttaaa
    16621 cggtgttcaa aagctttcct ctgcggtaat aagtccagcc aaacgaaaaa taggaggagc
    16681 tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca accttttaaa
    16741 attacatatt tttccataga acttgcattt gatccaagat ttcgatgaca tcttttgtca
    16801 agaagagaga gcgattgggc aagtcacctt caatcatgtg taccatattc tttatttcgt
    16861 aattcattgc ttcagatgta ttcccgcttt cgatgaattc ctttgtacca tctgtaaata
    16921 agatctccgc acgatcagca cgcgggtaat cagcaatcgt cacataaccg ttctcaaagg
    16981 caacgatacc gacttttggc atttttgcct gaaaagtcaa gctgacagca gcaagttcat
    17041 tttctttgtt acgcaagata gtgacggatt gttcgtccac gcctgtttca aaaggaacca
    17101 ttgtagaggc aactacttca ggttgagagc tcaaaaagaa acgagcgaaa gatacagcat
    17161 acgtaccgat atccaacaga gctcctccag ctaattctgg attgaagaac cggtttttag
    17221 ggtctggttc tttgtaactg ccgaaaggtg cttggatcat ttttaaagcc cccagttttc
    17281 ctgtatccat tattgagcgt aactgctgat aaagtggcat gttgaagatt gtcatcgctt
    17341 ctgctaagat caaattcttt tcttctgcta agcgcatagc gtcagccagt tcttttttat
    17401 tcatcgtgat ggctttttca catagaacgt gtttgcctgc ttccaatgct gccaagatat
    17461 ggtcgatatg ctgtctattt ggtacagcaa tgtacacgat gtcgatttca gcatcgtcta
    17521 acatttcttg atagcttccg taagcttttg gaatattgta acggtgagcg aagttttccg
    17581 ctttttctga agttctggaa gcaactgctg ttagttcgct cgtctcttga ttgaagcttt
    17641 cggcaaactc atgtgcgatg gtaccaagac caacgattcc ccattgatat tttttcatct
    17701 gaaaacgctc cttcatagtt ggattgagtt tcttgtctgt ctattattat aacaaaaatc
    17761 acgaagaata aagaggagag gacttgaaac attaaaaaaa atgaaagcag cgatttaaga
    17821 ggtgcttttt gatcaaaact taagtagaat agttaaaagg aggggataaa cgtgcatcaa
    17881 gtatatattg atcttattat gagtgtatta ttagaacagt ttgaaactga aaaaaatttt
    17941 ttagatgaat acttgctgat cagtccagaa caatggcaaa aatggaaaga tggcgagaaa
    18001 aatctgccat ctgaagcgat gcagaaagta aaaaatcttt tcactgatta tgaatggatg
    18061 ctaacgcaaa agattttgcg tcaaacgatt atttttcccg aaaaacgaaa cgttgccgtt
    18121 gcagagtaca agcaaatcaa aacacgcatc gcgcaaaaat ggcttagcag caaagcagca
    18181 aaagtcgagc tgatcccttt gaaaaataat cagcaggctg gtagatacct tgacttgaaa
    18241 gtaagtattc aatatgatga atggggctat gatgatattt tgaattttcg tctgccagca
    18301 gatgttcaaa aacagatcga aggagaaaaa atcgaacttc tcgattgggt gaatgaaaat
    18361 ctggaagaaa cttacgttca tgaggcgaat ggggaacgtg aagaacaacg aaaggatttt
    18421 tcaagtgaat aaatttttag gatttggagc agttgtgatt atgatcgcag caatgggcta
    18481 tgttattttt agcgatgatg attcgcatgt tcaagccggc aatcaagcag tcgaacgaac
    18541 aagcaccaaa acaactgcta gtaaagaaaa gaacagtcaa acaaccagca gcaaggtaaa
    18601 ggacaaagaa acaaaagatc gtacgtccaa acaattgcca gatgttaaaa cagacgattg
    18661 gaagctagtt ttggttggtc cagatcacaa attagaaaaa gaaattgatg agtctactca
    18721 attggcatca ttaagtaacg gatacttgat cgacaaacgc attgctccag aatatgaagc
    18781 atttgccaag gctgctgaag cagctggatt tccattagtg atggtctcag cttaccggtc
    18841 tgtatcatcc caacaacaag ttttcgaaca aaatgtccaa gatgtgatga gtcgtcaagg
    18901 tgtatcagaa gaggaggcga caaagattac taaagaaacg attacggagc caggatacag
    18961 cgaacaccac acaggtctgg cagtcgatgt cgtcgatcaa aactggtaca acagttatcc
    19021 ttctactgta ttggatgcaa gttacggaga tcagcctgga gctaaatgga tcgcagataa
    19081 tgctgcgcag tatggattta tcgttcgtta tccaaaagga cacgaagata taactaagat
    19141 cacatatgaa ccttggcact tacgttatgt cggtaaagaa aacgctgagt atatcaccaa
    19201 acaccaattg acgctagaag aatatctgaa tgaattaaat gaaaagtagg aattgatcat
    19261 ggcgaaaaaa aagtggaaaa agcaaaagcg caagaatatc cgttggccag cagtggttgc
    19321 cggtgtagca gtgatactct tagcctttgt tttttccttg aagaatctgt cttctccttt
    19381 tactgataat cagcaagaaa atgaagcgca atcccatcag caatttattg aacggcttgt
    19441 tccgcatgcc caagaactac aagatggtta tggagtgctt cctagtatca ttttaggaca
    19501 agctatatta gaatcaaatt ggggaaaaag taccttggct agcaaataca ataatctttt
    19561 cgggatcaag gcttatgggg atcagaagaa agtaagcctg gaaacaaagg aatttgttaa
    19621 cgaagaatgg ataactatcc aaggagactt caaagtgtat gattcatggg aacagtccat
    19681 ggatgaccat acacaactgt tcgtccaagg agtagattgg aatcctgcgc tgtatgaaaa
    19741 agtgattacg gccaccaact atcaggaagc cgcacaagca ttgcaagatg caggttatgc
    19801 gacagacccc ggttatgcgc aaaaaatcat ccaggtcatc gagacgtatc aattgaatca
    19861 gtatgaccgg agatagcaaa ggagaagtgg ctgatggcag aacaatttac gccagaaatc
    19921 atgacggaat tgagaaaaga tatcgtaaga gtaccggaaa tcattttaga agcaagcggg
    19981 atcaagatat ttggaaaatt gatccgttcg atcatcttta ctacggatat tgcgatcata
    20041 cgtaatacaa atgcgaatgc agtaattgct gtgtatcctt ttacacctca tccagcgatt
    20101 actaaaagca ttattgaggc agctgacatt ccagttttct ccggtgtagg tggtggtttg
    20161 acacaaggtg ctcgttcttc ctacatgagc atgttcgcag aagcgcaagg ttcgttaggg
    20221 gtcgttttga acggtcctac gcctttagag acagtaaaag aagtttgtcg agtagttgat
    20281 attccagtta tcagtacggt aacgtcgaaa tatacagaaa tagatgaaaa gttagcagct
    20341 ggtatttcga ttatcaatat cagtgcaggt aaagaaactg cagaagcagt acgccatttt
    20401 cgtaaaaaat atcctgaact tcctatcatt gcgacaggtg gtccaacgga agaaagtatt
    20461 gctgagacca ttcaagcagg agcaaatgcc atcacatata cgccaccaag tagcggagag
    20521 ctttttagtc gtaaaatgga aaaatatcgg gaaactgaga aaaagaatca tgaataacag
    20581 gtgagaatca aaagacgagg aagagcagat agtggctctt cctcgtcttt tgtactccag
    20641 tactatttgt tcagaattca aaaaaaagag aaattttttt gtttttttac ctgtaatgta
    20701 cataagaaga gttaaaacct tttgttatta ttagcattct aacaaaaaaa tgtcttgatt
    20761 tattgaagcg ctttctatag aatgaaaaac gaataaaaac taaaaaaggc acccgacaat
    20821 ttagtagcag ctaaattgtc ggggcttgaa aagtcagcgg gaactgacca atcaagttgc
    20881 catagtcaat gcttgtgcat cgacgtgtat attgtaccta atttcataca gaattactag
    20941 ttatttttct gttttttagg gaagagcgca cacgaagaag catgcttatt tagctatgtc
    21001 aaggatattg ggcagttgtc tgatatcctt ttttctttgc gtaattatcc tcccatcatt
    21061 gaagcaagaa gtgtttttag cgatgaaaga agaatcagca ctatctaatt tttcttgaaa
    21121 gtattgagtt gttcatccta ggcagaacga taaggcaagt cttggttttt attccccttg
    21181 aaaaagatcg tttaataaat atgttctgta taaaaataca gtatattgaa aaataacaat
    21241 ggaagtagag gtgcagagaa tgaagaaaag aatgagaatc atcttagctt taagtgttat
    21301 gttattcttc ttgacggctt gtgggcaaga agatgacaaa aaaatcacgc tgaacatggc
    21361 ttggtggggt tcgcaagtcc gacacgatgc aacagtaaaa gtaatcgagc tttatgaaga
    21421 aaaaaatccc catgtgacga tcgattatga attttatgat tatgaaggat attacacaaa
    21481 attaggtaca ctagcagcat cagatgatat ctgggacatt ttccaactga acaatcaatt
    21541 tcctcaatac atcaatcaga ttgaaccatt agatgaatat gtggagcaag ggataattga
    21601 tatttctaat gcagatgaat cctatttagc aacgacgact tacgacgggc aattagttgg
    21661 actgtctaac ggggtgaatt cgtttgcgat tgcttacaat aaaaatatat ttgaacaatt
    21721 aaatatccct gaaccagctt caaattggac atgggatgaa tttgaacaaa tcagctatca
    21781 aatcactgaa gaattaggaa tctatgctat ttcaagattc gaagatttta tagctggctg
    21841 tattattcaa ataccacaag ttgagaaagg attgaatttt tataatcggg aagatctatc
    21901 taacagcttg ggatttgaaa atccagacta tctaacagat tttttccagc tgcgtaaaaa
    21961 tctggtagac aaaggggccc atccagaacc aggtggtgga gctgcaagta cgacggatct
    22021 gtcttttcag gcggttaggg atagagaagc ggcaatggtg tttctatcaa gtaaccagtt
    22081 gacagaaata ttgtcagggg ctcctgaaga tatggagatt cagttgatta atccacctcg
    22141 cagaaaagca gatggagaat ctggtatccc tttacgttct tcgcaaatgc tgagtatggc
    22201 aaaaagctca gcccataaag aagaagctgc taagtttatt gatttctttc aaaacagtga
    22261 agaagccaat gaaattttaa aaggagaacg tggtgtacca atcatgtcga atattcgaga
    22321 acagctcgct cgtgcagatg atgcaacttt gcaagcgaca tttgattatt tagaaatgat
    22381 ttccgatatg gacaacggcg aagtgaatgt ctttgatagt gctttgaatc cggaaattca
    22441 agaccaatac aatcttttag ttgaaaaagt catttatgaa gaagtaacag cagaaggaag
    22501 gcatgaaaaa agaaaaacta aaacggttct tggatcaaga caatgtggtc ggttacgtat
    22561 ttgcttctcc attcatccta ggatttctat tgataacagt gataccgatg atcttgtcac
    22621 tttattattc gttcaccgat tatcgtttgg gaaatccagt ctcatggatc ggcttggata
    22681 attatataaa gatgctgagt gatgcccgtt ttttgaactc catccgagtc acttttctct
    22741 atgtatttgc atctgtacct gtgaaactga tctttggtct attgattgct tatcttttaa
    22801 cgaggaatat caaaggaacg actttctttc gttctttgta ttatgtgcct agtttgatcg
    22861 gtggcagtat ttctatcgct ctagtttggc gggagttgtt ttctaaaaac gggttagtga
    22921 atattttcct ggaacgatta ggcgcacctg cgctttcctg gtttagcgat caaaaactgg
    22981 tcatgatccc tcttgttttg atgtctgctt ggcaattcgg ttcggcaatg atcatctttg
    23041 ctgcaggtat caaagagatc ccaggctcgt attatgaagc ggcagagatc gatggagcga
    23101 acaaatggca gacgttttac aagatcacgt tacctcttct ttcgccgatc attttgtata
    23161 acttggttat gcagacaatc gcggcgttta tgacttttac gcaggcattt atcattaccc
    23221 aaggtggtcc gaatgatgcc acgaactttt atgctcttta tgtgtataac caagcttttt
    23281 cttttaattc tatggggtat gctagtgcta tttcgtggat catgctgttg attatgggag
    23341 ctgtcacctt tctgattttc aaatcatcaa aaaaatgggt cgttgtgtga atgagaggag
    23401 gaagaaatga ataatacagt aacgaaatgg tttcaatacg gttttatttt gctattaggt
    23461 ctagtcatga tttatccggt cttatggatg atcgcaggtt cttttaaaac ggatcaagag
    23521 attatgagtg gttcgctgaa tttgattcca gagacttttc aatgggaaaa ttacgcaaaa
    23581 ggatgggcgg ggtttgctgg aatttcattt tttactttct ttaggaattc gtttgtcatc
    23641 tctgcagttt cgacgatagg gacagttctg agctcgactt gtattgctta cgcattatcg
    23701 agaatcgatt tcaaaggaaa aagattctgg ttcgtcgtca tgttagtaac gatgatgatt
    23761 cctgcacaag tgattttgat tccgcagttt attatttaca atcggctgaa tttggttggg
    23821 acgtatgtgc cgctgatttt accacatttt tttggacaag catttttcat ttaccagatc
    23881 atgcaattca tggtcagcat ccccaaagag ttagatgaat cagcgattat tgacggatgc
    23941 agcaagtata gtgtctttac caaaattatt tttcctttgc tgaaaccttc gatcatcacg
    24001 acgatcatca tccagtttta ttggaaatgg gacgacttca tgggaccatt gatttattta
    24061 aacaagccga gatcttacac ggtatcgatt gccatcaaat tatttgcaga cgctggatct
    24121 agtacgactt acgcgagtat gtttgcgatg tctacgttgt cgctgctacc ggtttttctg
    24181 attttcttat tcttcaataa atatcttgtc caggggatca gtacaagcgg tctgaagggc
    24241 tgaatatgaa aaagccaata gaagagatgt ttctttacaa gctcaactac tatgtctgtc
    24301 ggattttttt aaccagtctt atcagtaatg ctcttttcgc tttagttcta ggatgtttgt
    24361 tcactttagg attagtgctg tctgctcgtg ttgtctatct gatttatttt ttgatgatgt
    24421 atccttctta ttatctatct gtccattact tggcagaaaa aaaggtatgt tggaggaaag
    24481 ggaaaatcaa acggatgtgt ttcatcatta cgttgtttca gctgtttgtc attatttttg
    24541 cagttgattg gtatttatta gtgggaaaga ttggagcttg gtatctgatt ccttttctat
    24601 tgtgtctgtt gctttttgta tcccatagtt tttttagtac gctaattgat atctgcaaag
    24661 aagataaagc agatagtctt gtttatttga tcaaaaggaa tatctggctg ttgtcaaaaa
    24721 acgtcaaggg gtttctgtgg tatttatgcc ttttgtatgg actgttctgg tgtatacagc
    24781 ctgatttttt actcactgca ttactgtatc cagggctgcc atttgtcaca gtattttgct
    24841 taaaagaaaa ggagaatgac tttaaatgaa gaaaaaatat gtacaagtcg gacttggtgg
    24901 gagagcgcgt tttttttatg aggcaattgc ctctacgtat catcaaactt gtgaactagt
    24961 cggtttttgt gatgtatctc aaacaaggat ggattatgca aatcggatac ttgtgaacaa
    25021 gtatggacag aaaaaagtaa agacatgtat gccatcacaa tttgagcaac tactggatga
    25081 cgtacggccg gctgaagtca ttattacgtc agtagatcga acgcatcatg attatatcat
    25141 tcgtgcaatg aaaaaaggat atgatgttat ttgcgaaaaa cctctgacta tcgatgagga
    25201 aaaagctcaa gaaataataa cggtccaaaa acagacggga agaagcctgc gagtcacttt
    25261 taattatcgt tatgcaccgt atcacacgaa gataaaggaa ctgatagaaa gtgatgtgat
    25321 tggtgatgtg ttttctatcc acttcgaatg gctgttaaat acgcaacacg gggcagatta
    25381 tttcagaaga tggcatcgaa ataagcacaa tagcggcggg cttcttgttc ataaatctac
    25441 ccatcatttt gatctagtga atttttggtt gaacagccgt ccccaaaagg tatttgcatt
    25501 cggtggatta aacttttatg ggatgcataa tgcggagaaa cgcggagtag aaatatttta
    25561 cagtcgatgc tatggaagcg aactagctaa agctgatcca tttgctatag acctgtcagc
    25621 caatgaggaa ttgcgagaaa tgtatttaga agcagaaaaa gacagtggat atattcgtga
    25681 ccgcagtgta ttttccgatg atatctctat tgaagataca ctaggcgtaa cggtcaaata
    25741 tgaaactggt gcgattttga cgtattcgct aaacgcgtat atgccgtggg aaggattcaa
    25801 tgtttctttc aatggcagca aaggacggat cgaaatgaaa gtgatcgaaa agtcttacat
    25861 caatgcaggt ggagataaaa aggatgaagg agcagccgtt cttcagtcca tccatgtcta
    25921 tccgatgttc ggtgagccat atgaggtaat cgtcgaacaa gaagcgggcg gacatggcgg
    25981 aggagatccg cgtttattag atgatctatt tggtgaagaa aaagaagaag atccgtggaa
    26041 tcgggcagca actcacgtag atggtatttt gtcgattttg acaggaatcg cagcaaatca
    26101 ttctattgct tcaggaaaag cagtagtgat tgacgagctt gtcagcttaa aagaataaga
    26161 atagatttac ggaaattact tcccgtagtt tataaaatct tgccttattg ccgataggct
    26221 gtcttttgaa cactatttat tcggaaaaag gggctatgac atgctttttg tcatgccttc
    26281 tttttatgtg ggctgctttt ttagatagga gaaaataaaa ataactattt aaacccgaac
    26341 aaaaaggaat aatgcattaa aaatcattag attttacaaa aaaataagta aaccacgttg
    26401 tcagtttcag aaagtagtgt tagactgttt tcaatatttg aaacaacatt ttttattcaa
    26461 atatggcaaa ctgccttcac gtgcaaaaca gttgcaagaa aatgaattta cactattatt
    26521 agttttacat acctacttat atatcgtcat aatatggatg acagtttcta gccagtaccg
    26581 taaatgctgg actataagta aaagattggc tatttcaccc ttcttttctt atgataaagt
    26641 ggagggtgtt tttctttaac agaaacatag taggtttttg cacaaaagcg gttatttcgg
    26701 tattaataaa tgataaagga gcaaataaag tgaaagaatt agttgaacgt attcaaaaag
    26761 acggacgtat attaggcgag ggcgtattaa aagtcgacag cttcgtcact caccaagtag
    26821 accctgtatt gatggaacaa atgggaaaac ggtttgcaga agtatttaaa gaacaaggaa
    26881 tcacgaaagt tgtgaccatt gaagcttctg ggatcgcacc agctttattt gctgcaagag
    26941 aattagatgt tccaatgatc ttcgctagaa aagcaaaaag tttgacgatg gatgaagaat
    27001 tattgacagc ttctgtttat tcttttacga aacaagtgac aagtacgatt tctatttctc
    27061 gtaaattttt atcttcagaa gacaaagtat tgattatcga cgatttcttg gcaaacggac
    27121 aagcggcaaa aggattgatc gaattatgcc agcaagctgg agcgcaagta gaaggaatcg
    27181 ggatcgtcat cgaaaagtct ttccaagatg gacgtcagtt acttgaagaa atgggattga
    27241 gagtcgtttc tcttgcgcgt attgcttcct tgacgaatgg acaagttgag tttcttgagg
    27301 aggacgcata atcgtggaaa aagaaacaca aaatggaaaa gctgcggttt tagggctgca
    27361 gcatctgcta gcgatgtatg caggagcagt agcggtgcct cttttgatcg gtacaggact
    27421 aggctttaat caagaacaga tgacttattt gatttcaatt gatatcttta tgtgtggatt
    27481 agcgacatta ttgcaattga cagttaaccg atttttcgga attggcttgc ctgtcgtttt
    27541 aggatgtgcg atccaagcag tagctccact gatcttgatc ggaaccaatg aaggagtagg
    27601 agctatctac ggatctatta tcgcttctgg gatctttgtt gtgttagttt ctggtgtctt
    27661 ttctaaaatt aaaaaactat tccctcctat agtaacagga acggttatca ccgttattgg
    27721 tttgactttg attcctgtag caatcgagaa aatgggtgga ggtaatgcaa cagcagctga
    27781 ttttggtgac aaaacaaatc tgctacttgc atttgtgacg attctcctaa ttgtaggcgt
    27841 gcaaatgctg gcaaaagggt tcattcgttc aatcgcggta ttgattggtt tagtcggagg
    27901 aagcatctta gctgctttca tgggaatgat tgatctgagc gcgattggag atgcgccgtt
    27961 gttccatgtt ccacaaccct tttattttgg taaaccaact ttcgacgtat ggtcgatttt
    28021 actaatgatc atcatttcca tggtcagcat ggtagaatca acaggtgtat attttgcact
    28081 tggtgacatt actgggaaaa atgttggaga agaagaattg aaaaagggat atcgtgcaga
    28141 aggacttgcc gttatcttag gcggtatttt caatacattc ccttatactg gcttttctca
    28201 aaatgtcgga ttggttcaac tttcgggaat caaaacaaga aaacccatct atttctctgc
    28261 attcttcttg attgttttag gattattccc taaaatcggt gcgttagcac aaatcatccc
    28321 tgagccggta cttggtggag gaatgttagt catgttcggg atggtggcag tccaaggaat
    28381 ccgtatgctg ttggaagttg atttcagcaa tgataaaaat ctattgattg ttgctgtttc
    28441 tgttggattt gggctgggtt tcaatgttac gccgacatta ttcagtcaaa tgccgcaaac
    28501 attgcaaatg ttcactggaa atggcatcgt aatgagtagc attacggcta tcatattgaa
    28561 tttgatcttt aatggattga aaaaagatga agaaatattg gaagaagcct tcaagaagta
    28621 atagagcaaa agaaaggttg tgaaatcagc gttttgacct gattgttaga gaaacaaacg
    28681 gaaaaatagt tcctcgtatt ttgccgtttg tttgagctaa taccgagggt cagctgattg
    28741 aacaccgttt atccataaaa aggaatgtga cctaactttt gtcacattcc tttttttcat
    28801 ctttaataaa cacgaacaat aaaatgtttt tctttgttaa cattcgaaat ttctttgaca
    28861 tcaaaaaaag tgattgttaa aatatgaatg tagaaaaact gggaggaaaa acaatgacag
    28921 caaaaatctc tgtgattatg gggagtactt cagattggga aacaatgaaa gaagcatgtc
    28981 aaatattgga tgaattcggt gtgtcttatg aaaaaaaggt cgtttctgct catcgaaccc
    29041 cagatttgat gtttgaatat gcacaaaacg ctcgaaaaaa aggaatcaaa atcattattg
    29101 caggagcagg gggagccgcg catctgccgg gtatggtcgc atcaaaaaca actttgccgg
    29161 ttatcggtgt accggtccaa tcacgaacat taaatggttt ggattcgctt ctttcgattg
    29221 tccaaatgcc tggaggagta cctgtagcta ctacagcaat cggaaaagcg ggagcaatta
    29281 atgctggact acttgcgata cagatgcttt ccatgtatga tttagaattg gaggctaaat
    29341 tggctgaaag acgttcaatg ttggctaaaa ctgttataga aagtagtgat caacttggtt
    29401 aaacctttgt tgcctggttc aacgatcggg atcgtcggtg gcggtcaatt aggtcgtatg
    29461 atggcgatta gtgcaaaaga aatgggcttt aaagtaggtg tgctggatcc tgtgagcgat
    29521 tgtccagccg cacaagttgc agattggcac attgaaagtg tctatgatga cacctttgcg
    29581 ctgaaagagc ttgccagaag aacagatgtc attacctatg aattcgaaaa cgtcagtgta
    29641 gaggcgttaa atgcgatttt acccatgtca tttattcctc aagggacaga cttgcttgcg
    29701 attacgcaag accgcctgtt agaaaaatct tttttggaaa cgaataacat cgtgattgct
    29761 ccttatgcaa cgatcgtcag tccgacggat atccaagatg cgattgacgg gatcggctat
    29821 ccttgtgttt tgaaaaccac tcgaggcggc tatgatggca aggggcagta tgttttgaaa
    29881 agtcgggcgg atctagctcc tgccatggac cttttaagag aaggaacctg tgttttagaa
    29941 gcatggatcc cgtttgaaaa agagatttcg atcatggtgg caggaaatgg tcaaggagat
    30001 ttcacgactt ttccggtcgt ggaaaacatc caccacaaca atattcttca tgaaacgatt
    30061 gctccagccg cgattgatca ggatgtgatc gaggaggctg agcgtattgc ccgtgtgatt
    30121 gcagaagcag tgagcctttc aggtgtttta ggggtagaaa tgtttttaac aaaaacaggc
    30181 gggctatatg tgaatgaatt agctcctcgt cctcacaatt ctggacatta ttctattgaa
    30241 gcttgttcta tgagtcagtt cgatgcccat attcgaggta tttgtggttg gccattagga
    30301 gaaaaagtac gtttgataag caatgcagta atggtcaatg ttttgggaga acaactgctg
    30361 gaaagctatc gcttgattga gaataagcca gactggcaat tccatttcta tggaaaaaaa
    30421 caagcaaaaa aaggtcgcaa gatgggacat atcactatct tgacagaaga catccagcaa
    30481 acgctggaag aaatcaaaca aacgaatatt tgggattaga ggagaaaaaa catgttagaa
    30541 cgttatacac gccctgaaat ggggaatatc tggacggaag agaacaaata ccgtgcgtgg
    30601 ctcgaagtcg agattttagc agatgaagct tgggcagaac ttggtgaaat cccgcaagaa
    30661 gatgtgaaaa aaattcgtga gcatgcatca tttgatatcg atcggatctt ggaaatcgaa
    30721 aaattaacac gccatgatgt cgttgcgttt acacgagcgg tttctgaaac attgggagaa
    30781 gaaagaaaat gggtccatta tggtctaaca agtacggatg tcgtagatac tgcttatggc
    30841 tatctcatca agcaagcaaa tgatatttta cgggaagact tgaaacgttt tacggaaatc
    30901 attggcgaaa aagcaaaaga acacaaatat acagtgatga tgggtcggac acacggtgtc
    30961 catgctgaac caactacttt tggattgaaa cttgccacat ggtattcaga aatgaaacgg
    31021 aacatcgaac gatttgaaca tgcggcaaaa ggagtagaag caggaaaaat ctcaggagca
    31081 gtgggaacgt tcgcaaatat cccgccattt attgaagagt atgtatgtaa acaattgggg
    31141 atccgtccac aagaaatctc tactcaagtt ctccctcggg atctacatgc tgaatatttc
    31201 tcagcgatgg cgcttattgc tacaagtatt gaacgttttg caacagagat acgcggattg
    31261 caaaaatccg aaacacgtga agtagaagaa ttttttgcga aaggtcaaaa aggttcttct
    31321 gctatgccgc acaaaaggaa tccgatcggt tcagaaaata tggctggtct agccagagtg
    31381 gtgcgcggac acatggtcac tgcctatgaa aatgtcacat tatggcatga aagagatatt
    31441 tctcattctt cagcagaacg aatcatcttg cctgatacca cgattttatt gaattacatg
    31501 ttaaatcgtt ttggcaatat cgttaaaaat ctaacggtct ttcctgaaaa catgaagcgg
    31561 aatatgtctg caacatttgg tttgatttat agccagcgcg ttatgctgaa attgatcgat
    31621 aagggaatga caagagaaga agcttatgat cttgttcagc caaaaactgc gtacgcttgg
    31681 gacaaccaag tgcagttccg cccgctgtta gaagaagatc caaccatcac atcggtatta
    31741 tctaaagaag aaatagacga tgcctttgat tatcatcatc atttgaaaaa cgttgacgtg
    31801 atattcaaac gagtaggttt ggaataaaga agagtgagtt gcaaaattca attaaaatag
    31861 aatagcaaaa ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttagttta
    31921 ttatattttc ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc
    31981 ctctttacat acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa
    32041 tgctggttat ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca
    32101 ttgaataaga tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg
    32161 attgaaagtt cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca
    32221 cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat
    32281 aaataaacgt ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt
    32341 aattctgata aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc
    32401 gaacccatta tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa
    32461 cgcttgttga tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa
    32521 tgccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg
    32581 aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca
    32641 tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg
    32701 agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag
    32761 accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc
    32821 tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag
    32881 caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt
    32941 tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct
    33001 actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac
    33061 aacttgcaaa accggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa
    33121 ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg
    33181 gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat
    33241 gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt
    33301 tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc
    33361 gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca
    33421 aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg
    33481 aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat
    33541 tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat
    33601 caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt
    33661 gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac
    33721 ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct
    33781 atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga
    33841 ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga
    33901 cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca
    33961 aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg
    34021 aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac
    34081 ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca
    34141 gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc
    34201 atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt
    34261 ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag
    34321 ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc
    34381 gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt
    34441 ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc
    34501 ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg
    34561 gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc
    34621 cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg
    34681 ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga
    34741 aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac
    34801 cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg
    34861 caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg
    34921 gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt
    34981 ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg
    35041 atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg
    35101 attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa
    35161 tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa
    35221 ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg
    35281 ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag
    35341 tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag
    35401 tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga
    35461 aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag
    35521 aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa
    35581 tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg
    35641 ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg
    35701 cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta
    35761 gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt
    35821 ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg
    35881 cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg
    35941 atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata
    36001 aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga
    36061 cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg
    36121 tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca
    36181 tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac
    36241 tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg
    36301 ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga
    36361 cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag
    36421 cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa
    36481 agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg
    36541 gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag
    36601 tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa
    36661 ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca
    36721 gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt
    36781 ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa
    36841 tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt
    36901 ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga
    36961 ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt
    37021 aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat
    37081 cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat
    37141 tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg
    37201 aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat
    37261 ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa
    37321 tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc
    37381 taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat
    37441 cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag
    37501 agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt
    37561 gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt
    37621 gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc
    37681 atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat
    37741 tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct
    37801 gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt
    37861 caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa
    37921 agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa
    37981 atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca
    38041 agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg
    38101 gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg
    38161 gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg
    38221 actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct
    38281 tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg
    38341 tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc
    38401 ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa
    38461 aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat
    38521 ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc
    38581 agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg
    38641 agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag
    38701 gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat
    38761 ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac
    38821 aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta
    38881 tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg
    38941 gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta
    39001 taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc
    39061 gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga
    39121 tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt
    39181 ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa
    39241 agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt
    39301 gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc
    39361 tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat
    39421 tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat
    39481 tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat
    39541 ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca
    39601 ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa
    39661 ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa
    39721 acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca
    39781 cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag
    39841 gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc
    39901 aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc
    39961 ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt
    40021 gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt
    40081 tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg
    40141 atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg
    40201 tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat
    40261 cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa
    40321 ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac
    40381 gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg
    40441 attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc
    40501 atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta
    40561 gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa
    40621 aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg
    40681 gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa
    40741 gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt
    40801 gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag
    40861 acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa
    40921 caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat
    40981 gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc
    41041 atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact
    41101 gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt
    41161 gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg
    41221 gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc
    41281 agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt
    41341 atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga
    41401 ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct
    41461 aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac
    41521 gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc
    41581 ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca
    41641 gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag
    41701 tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg
    41761 atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc
    41821 ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg
    41881 gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt
    41941 gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg
    42001 cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa
    42061 gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac
    42121 aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt
    42181 ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac
    42241 ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc
    42301 aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt
    42361 tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc
    42421 tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct
    42481 tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa
    42541 aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg
    42601 tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga
    42661 tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc
    42721 tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt
    42781 tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt
    42841 gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt
    42901 agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta
    42961 ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca
    43021 accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg
    43081 gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta
    43141 ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg gattttttga
    43201 aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc
    43261 agctaaatat caaacgacac caactggctt ggcttctgct tggattttac gccatccagc
    43321 caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc
    43381 ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat
    43441 tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa
    43501 cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt
    43561 atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat
    43621 attcttttgg agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat
    43681 tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt
    43741 ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag
    43801 aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg
    43861 acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct
    43921 gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa
    43981 ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc
    44041 aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag
    44101 tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt
    44161 gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa
    44221 agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa
    44281 tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag
    44341 gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc
    44401 ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt
    44461 cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca
    44521 cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa
    44581 agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc
    44641 gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac
    44701 cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt
    44761 tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt
    44821 taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga
    44881 aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt
    44941 ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct
    45001 gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga
    45061 ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt
    45121 cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga
    45181 gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt
    45241 tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata
    45301 ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca
    45361 gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgcg
    45421 gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca
    45481 atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg
    45541 atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata atcattgata
    45601 tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa
    45661 aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa
    45721 gaaataatgg catccaaact gttataaata aaatggggag taatctgagc ttgcaataca
    45781 cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt
    45841 agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc
    45901 agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga
    45961 ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa
    46021 atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc
    46081 gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac
    46141 agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct
    46201 tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt
    46261 agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga
    46321 atatcattcc taagttcttc taggatattt tcctcattga actcatctac ggttacttgg
    46381 ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca
    46441 tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac
    46501 agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca
    46561 aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt
    46621 tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg
    46681 taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca
    46741 gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat
    46801 catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt
    46861 ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc
    46921 gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg
    46981 aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc
    47041 atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt
    47101 ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga
    47161 atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg
    47221 gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat
    47281 gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa
    47341 aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg
    47401 ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc
    47461 agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa
    47521 accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta
    47581 agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg
    47641 gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg
    47701 aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa
    47761 agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc
    47821 aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct
    47881 attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt
    47941 acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg
    48001 aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa
    48061 gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca
    48121 cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa
    48181 gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat
    48241 tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac
    48301 ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac
    48361 gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac
    48421 aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc
    48481 tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac
    48541 caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca
    48601 ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat
    48661 gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa
    48721 gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa
    48781 gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat
    48841 ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat
    48901 tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct
    48961 aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca
    49021 aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa
    49081 tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg
    49141 gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat
    49201 ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac
    49261 taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag
    49321 gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt
    49381 tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg
    49441 aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg
    49501 cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag
    49561 gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa
    49621 tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag
    49681 agcatattat tcgcgggatg gtgggcagag atttagttga tcgttatcct gaaagaaaac
    49741 cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc
    49801 acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa
    49861 tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt
    49921 atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg
    49981 ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac
    50041 tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc
    50101 aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga
    50161 tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac
    50221 aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc
    50281 caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg
    50341 cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt
    50401 gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg
    50461 cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag
    50521 gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat
    50581 ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg
    50641 atgacggatg gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc
    50701 catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg
    50761 tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc
    50821 caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca
    50881 tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga
    50941 ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt
    51001 ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc
    51061 cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa
    51121 tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc
    51181 ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct
    51241 tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt
    51301 ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg
    51361 gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga
    51421 gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa
    51481 gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact
    51541 tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg
    51601 aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg
    51661 atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag
    51721 aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct
    51781 aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca
    51841 ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg
    51901 gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca
    51961 ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg
    52021 tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa
    52081 aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt
    52141 agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt
    52201 tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga
    52261 ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat
    52321 cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata
    52381 tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt
    52441 ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac
    52501 aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc
    52561 gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc
    52621 aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga
    52681 ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc
    52741 tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca
    52801 acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat
    52861 ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa
    52921 aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga
    52981 aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt
    53041 tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt cagaataagg
    53101 gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt
    53161 ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg
    53221 gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga
    53281 gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg
    53341 caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt
    53401 ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa
    53461 ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac
    53521 tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta
    53581 tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt
    53641 gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac
    53701 aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt
    53761 gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga
    53821 gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca
    53881 ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa
    53941 gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct
    54001 acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat
    54061 cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa
    54121 gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg
    54181 gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa
    54241 ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg
    54301 gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa
    54361 gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca
    54421 attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta
    54481 gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat
    54541 ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat
    54601 tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta
    54661 aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata
    54721 cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa
    54781 aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg
    54841 taagtttgat tagaaaatag aaaaaatgca gatgaaaaat aaaacgcttt ccttattgtg
    54901 attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat
    54961 ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa
    55021 atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg
    55081 agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct
    55141 agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt
    55201 taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat
    55261 ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg
    55321 cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga
    55381 taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt
    55441 acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga
    55501 agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg
    55561 tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga
    55621 tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc
    55681 aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca
    55741 aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt
    55801 cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat
    55861 cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact
    55921 atctggtgac gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa
    55981 agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa
    56041 agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga
    56101 caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca
    56161 atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa
    56221 aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat
    56281 gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga
    56341 ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc
    56401 gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc
    56461 tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat
    56521 cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt
    56581 catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt
    56641 cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga
    56701 agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa
    56761 gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca
    56821 gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat
    56881 cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat
    56941 gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca
    57001 caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt
    57061 tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa
    57121 gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg
    57181 gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa attgccggat
    57241 atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg
    57301 atcaatgaag atgatacatt actagaagtt caaaatgaca aatcagtaga agaaatcccg
    57361 tcacctgtta caggaacggt taaaaatgtg attgttccag aaggtaccgt agcaaatgtt
    57421 ggggatgtat tagtagaaat cgatgcacca ggacacgaag acaatgaagg ggacagtggt
    57481 gtagccgcag aatcccaaac tccagctaaa ccggctgctg aacccactgt tgatacagaa
    57541 tcagctggat cgtcaagcga aggagtcttc caatttaagt taccggatat cggtgaagga
    57601 atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat
    57661 gatacattac tagaagttca aaatgacaaa tcagtagaag aaattccgtc acctgtcaca
    57721 gggacagtta aaaacgtgat tgttccagaa ggcactgtag caaatgttgg ggatgtatta
    57781 gtagaaatcg atgctcctgg acacaatagt gcaccgtcaa ctagtgcgcc atcagctgaa
    57841 gcaccaaaag aaaaagtgga aacatcaggt tcagcaagtg tcgttgaagc tgccgatcca
    57901 aataaacgtg tattggctat gccatctgtt cgtcaatttg cccgtgaaaa agatgtagat
    57961 atctcacaag taactgcaac tggaaaaggt ggcagagtaa caaaagagga catcgaaaac
    58021 ttcttggcag gtggtccaag cagtgcacca gcgaaatcag aagcgccaga agcggcagca
    58081 ccgaaagaag cagctcctgc agcagaaagc aaaccagctg caccagcaaa accattcaag
    58141 tccaacttag gagacttgga agaacgtgta gcaatgacgc caacacgtaa agctatcgct
    58201 aaagcaatgg tcaacagtaa gcatacagct ccacacgtga cattgcatga tgaagtagaa
    58261 gtgtctaaac tatgggataa tcggaaacga tttaaagaag ttgctgctgc aaatggtacg
    58321 aaacttactt tcttgccata tgtcgtgaaa gctttaacag cgactgttaa gaaataccca
    58381 gtattgaatg catctatcga cgatgcaaac caagaaatcg tttataaaca ttactacaat
    58441 atcggtattg caactgatac ggatcacgga ctatatgtac caaatgtgaa agatgcagac
    58501 cgcaaaggaa tgtttgctat tgcagacgaa atcaatgaga aagcaaaatt agctcatgac
    58561 ggcaaactat ctgcagaaga tatgcgcaat ggaacaatta cgatcagcaa tatcggatct
    58621 gtaggtggcg gttggtttac accagtcatc aactatccag aagtagctat tttaggtgta
    58681 ggaacgattg cacaacaacc aatcgtaaat gctgaaggcg aaatcgttgt cggtcgtgtg
    58741 atgaaactgt cattaagttt cgatcaccgt atcgtagatg gagcaactgc gcaacaagca
    58801 atgaataata tcaaacgtct tttagctgat ccagagctat taatgatgga aggatgataa
    58861 acaaatggta gttggagatt ttgctgtaga attagataca gttgtaattg gtgcgggacc
    58921 tggtggctat gtagctgcca tccgcgctgc tgaaatggga caaaaagtag caatcattga
    58981 aagagagtat attggcggtg tttgtttgaa tgtcggctgt attccttcaa aagcactgat
    59041 agctgcagga caccattatc aagagtcact agattctgaa atgtttggag taactagtga
    59101 aaacgtcaaa ctagattttg caaaaacaca agaatggaaa gaaaacaaag ttgtccatac
    59161 gttgacttct ggtgttggtt tcctattaaa aaaacataag gttgaaacaa tcgaaggaga
    59221 agcgttcttc gtagatgatc acacactacg cgtcattcat ccagattcag ctcaaactta
    59281 ttcgttcaat aacgcaatca tcgcaacagg ttcccgtcca atcgagattc caggatttaa
    59341 attcggagga cgtgtgttag attctactgg cggattagca ttgaaagaag tacctaagaa
    59401 atttgtgatc atcggtggcg gtgtgattgg tgccgaattg ggcggagcgt acgcaaactt
    59461 aggagcagaa gtaacgattc ttgaaggctc accacaaatt ttaccaactt acgaaaaaga
    59521 tctagtaaaa ttagtagaag atgatttcaa gaaaaaaggc gtaacagtcg taacgaatgc
    59581 aatggcgaaa gaagcagtcg ataacggtga cagcgtgaca gtgaaatatg cagtggacgg
    59641 gaaagaagaa tccgttactg ctgattatgt gatggtcact gtaggtcgtc gtccgaatac
    59701 agatgatatg ggcttagaac aagcgggtgt cgaagtagga gaacgaggat tgatcacggt
    59761 ggataaacaa ggacgtacga acgtacctaa catctatgcc atcggtgata tcgttccagg
    59821 ggcggcatta gcacacaaag ccagttatga agcaaaaatc gctgctgaag caatctctgg
    59881 caaaaaagta gctgtagatt ataaagcaat gccggcagta gccttcactg atcctgaatt
    59941 ggcttctgta ggtatgacga tcaaagaagc aaaagatgct ggtatcgaag caaaagcata
    60001 taaattccca ttttcaggga acggtcgggc attatcgctt ggtaaaacag aaggattcat
    60061 ccgtctagtt acgacgatcg aagataacgt gcttattggc gcgcaaatcg gtggagtcgg
    60121 cgcaagtgat atggtctctg aattagcttt agcaatcgaa tcaggaatga atgctgaaga
    60181 tatcgccttg acgattcatc ctcatccatc tcttggcgaa atcgtcatgg atgcttcgga
    60241 attagcatta ggtttaccaa tccatatttg atttgaataa tacaggcgaa acgcaactga
    60301 aaaatcagtt gcgttttgtt ttttagaatt atcaataata ttattatgta aacttaaaaa
    60361 tatgacaact tcttggttta ttaccacttc ctatgctaca ctgaagagga atatgaaata
    60421 ggagagttga agatgcaaga atatcagtat ccacttgatt tagattggac aacagacgaa
    60481 atggtcattg taatgaagat gtgggaagat ctggaacgtg cgaatgaaaa agggataaac
    60541 aatgaagctt ttttgaagac ttatcagcag tttaaaactg ttgtcaaatc cattggtgag
    60601 gaaaaacgat tgggacggga atttgaaaaa gcttccggtt attctttata tcgtacagtg
    60661 cagcaagcaa aaaaaaatcc aaacaaaact ttgagaatga acgggtgaga taaatggaac
    60721 agatgattga tcatatcaag tcctggcttg aagaagctgg tgagaaaatc aaatcttctc
    60781 aacaaacatt aacaatctcg caaaaatcaa atcgaacgga tctagtaaca aatgtcgatc
    60841 gaaacatcca gaattttcta atcgaaaaaa tccattcctt tgatccagag gcgaaaatat
    60901 tggcagaaga agaagggtgt aatcaattat cggatctcga tggaagagtc tttatcatcg
    60961 atcctattga tggcaccttg aattttgtga ttgaagggga aaatttttgt attatgctcg
    61021 ctgtttatga atccggcgtt ggaaagcttg gtttcattta tgacgtgatg agaaatgaac
    61081 ttttttgggg tggaaaagga ataggcgtgt ataaaaatga tgagccattg cctagaccag
    61141 caaataaatc cttatcggaa ggcttacttg gtgtgaatag ttatcttttc ggtcataata
    61201 aatatggaat ccgagaaatt gcggaaagca gcatggggac acgtatgtac ggatgcgctg
    61261 gtcttgaact agtggcaatc ttgaaaggaa accacattgc ttatatttca aaccttagcc
    61321 cttgggatta tgcagcggga aatgtcttat tggaagagtt tggtatggta acaagcggtc
    61381 tttttggtac tcctttgaca tttgaaaaaa gggaatacta tttagcaggg acttcttgtg
    61441 catatgcaga aatcgcagac cttttgaaaa aagaaggctg aaaaaaaggt agaaatcatt
    61501 cacaaaaatg ataaaaacga gcaaaagatt aattttctcg aaaaaatgaa aacattttac
    61561 tatatttcat gaaaaaagtc tgctataatt agcagtcgag taaaatttgc cctgtcataa
    61621 agaaccaata aaaaaggagt tagttacatt gaattataga aacgatatcc gtaacgtcgc
    61681 aattattgcc cacgttgacc acggaaaaac aacattagtt gatgaactat taaaacaatc
    61741 agatacatta gacgcacata cgcaattgca agaacgtgcg atggactcaa atgcgctaga
    61801 aaaagaacgt ggtatcacga ttttagccaa aaacacagct gtagactata aaggtattcg
    61861 cgtaaacatc atggatactc caggacacgc ggacttcggt ggcgaagtag aacggatcat
    61921 gaaaatggtt gacggcgttg tgttggtcgt agatgcgtat gaaggaacaa tgcctcaaac
    61981 acgttttgta ttgaaaaaag cactggaaca acatatcaca ccaatcgttg tcgtaaacaa
    62041 gattgacaaa ccttctgctc gtcctgagca tgtcgtagat gaagttcttg aactattcat
    62101 cgaattagga gcagatgatg atcagttaga ctttcctgtg atttatgcat ctgcactaaa
    62161 tggtacgtca agcttgtcag atgatccagc agatcaagaa ccaaccatgg cacctatttt
    62221 tgatacaatt attgaaaaaa tccctgctcc tgtcgataac agcgacgaac cattacaatt
    62281 ccaagtttca ttacttgact acaacgatta tgtaggtcgt atcggaatcg gacgtgtatt
    62341 tagaggaaca atcaaagtag gagaccaagt agcattaatt aaattagacg gaacagttaa
    62401 gaaattccgt gtaacgaaat tatttggttt ctttggacta aaacgtctag agattcaaga
    62461 agcaaaagct ggagacttga ttgctgtctc aggtatggaa gatatctttg tcggtgaaac
    62521 agttactcct gtggatcatc aagatgcgtt acctatcctt cacattgacg aaccaacatt
    62581 gcaaatgact ttcttagtta ataattcacc atttgccggt cgagaaggta aattcgtaac
    62641 cgctcgtaag attgaagaac gtttgatggc agaacttcaa acagatgtat ccttacgagt
    62701 tgaaccaaca aactctccag atgcatggac agtttctggt cgtggagaat tgcatttgtc
    62761 tatcttgatt gaaaacatgc gccgtgaggg atacgaattg caagtatctc gtccagaagt
    62821 tattgaaaaa gaaatcgacg gtgtaaaatg cgaaccgttt gaacgcgtgc aaatcgatac
    62881 accagaagaa tatatgggta gtgttatcga gtccttgagc ttacgtaaag gtgaaatgca
    62941 agatatggtc catacaggaa acggacaaat ccgtttgaca ttcttaacac ctgcccgcgg
    63001 tttgattggt tattcaactg aattcctttc aatgacacgt ggttacggaa tcatgaacca
    63061 tacttttgac caatatctgc caatgcttcc tggtcaaatt ggcggacgtc accaaggggc
    63121 gcttgtttcg atcgatacag gaaaagcaac gacttattct atcatgagta tcgaagaacg
    63181 cggtacagtc ttcgtagaac caggtacaga agtatatgaa ggaatgatta tcggggaaaa
    63241 cagccgtgat aatgatttga ctgtcaacat cacgaaagca aaacaaatga caaacgtacg
    63301 gtctgctact aaagaccaaa catctgtcat caaaaaacca aaacaattga cgttggaaga
    63361 atctttggaa ttcttgaatg atgatgaata ttgcgaagta acaccagaaa gcattcgctt
    63421 gagaaaacaa attttagaga aaaatgctcg tgaaaaagca tctaaaaaga aaaaataatc
    63481 tgtaaaagga tgaagagaga aaccagcttg ttataaaaaa tagctggttt ctttttttgt
    63541 ccaactcttt tctgtggaga agtcatcttt gtcaatggac ataaattcag aaaatacgcc
    63601 gaaagacgtt taaaagactt ttaaaaaact tatttctaga gagtttttgt ttcataataa
    63661 gataatgttt cacgaaagga gatcaacttg tccttcgaag aaaatcgaag taaaatggca
    63721 agagaaagga gcgggaagat gaacgactta ttaaaacaat actttggtta tgatgagttt
    63781 agacctggtc aaaaagaaat tatccagaaa gtcattgatc aggaaaatgt attaggaatc
    63841 atgcctaccg gaagtgggaa atcaatctgt taccagcttc cagctttact attagatggg
    63901 cttactgtgg ttgtgtcccc tttaatctct ttgatgaagg atcaggtgga cgcggcgaat
    63961 caattaggta tacccgccac ttttatcaat agctcgttag atggttacga aacagctaga
    64021 cgcttccaag aaatcgatag acagcagtac cgtttacttt atatcgctcc tgaacgtttt
    64081 atcatgccgg attttatcca agcaatgaag cgatggaacg tccgtatgat cgctattgac
    64141 gaggcacatt gtatctctca gtgggggcat gatttccgcc caagttattt acagatggcg
    64201 aaccagctag atcaactgcc aaatcgtcct gtaattgttg ctttgacagc tacagcgact
    64261 gtgcaagtag ctgctgacat aaaaagactt ttaaaaattc cagaaaacaa ccatattcag
    64321 actggttttg aacgagaaaa tctacgattt caagtaatta aagatcaaaa aaaagaacaa
    64381 tatctaatcg aatatttaaa gatcaacaaa aatcaatctg gtatcattta tgcagcaacg
    64441 agaaaagaag tagaccgtct ctatcatttg cttaaaaaat tcgatttttc cgtgggtaga
    64501 tatcatggtg gtctaaatga gaatgagcga acagaaatgc aagaagcctt tttatacgat
    64561 cgcttgcagc tgattgtagc gacaaatgca tttggtatgg gtataaataa aagcaatgtc
    64621 cgttttgtta tccattatca aattcctggc tctctagaag catactatca ggaagctggt
    64681 cgggcgggaa gagatggtct ttccagtgaa gccatcctgc ttttctctcc tcaagatatc
    64741 caagtccaaa agttttttat ccagcagtcc caaagagaag aagggcaaaa gcaaaaagaa
    64801 tatgaaaaat taaaagcgat gacagaatac gtgtatattg agtcctgtct tcagcaatac
    64861 atcctaaact attttggcga gacaagcagt ccgtgcaacc gttgtggtaa ctgtttagat
    64921 gacagagaaa tagtagaagt aacgacagaa gcacagatgg ttctttcttg cttaaaacgt
    64981 atgggtgaga actatggtaa acaaatgctg atgaaagtct tagcaggatc gaaagaacaa
    65041 aagctgcaag ctttaggttt tggacattta tctacctatg gactgatgaa aaaccaatcg
    65101 caaaaagaaa cgatgcagtt aattgaatat ttgatatcga atggctatct tctgaccatc
    65161 aatggtgagt atcctgtttt gaaagtaaca gaaagaggga tacaagtatt gaaaggacaa
    65221 gaatctgttt atcgaaagga acccaagaaa gtccagcagc tatcggatga agaaaccgat
    65281 acactatttg aagtgctgcg ggaactgcga acggatctag catcagaagc aggcgttccc
    65341 ccgtatgtcg tattttcaga ttccacattg aaagaaatga gccgaatacg tccatcaagc
    65401 agattggaga tgcttcagat aaaaggagtt ggccaaagca agcttgataa atatggcgaa
    65461 gcctttttat ctcggataaa aaatgcggac tctaacgttc tggataagta gaaagaacga
    65521 atacgtaaaa atgaataaaa acaactggtt aaatctgttt tttttgctcc ttatgaaaat
    65581 caaatatgtt ataattaagg aattagaatg ggaggtaagc acagatggat tctttgtcga
    65641 aaaaacttgc gatccaagta ttaaatgatg aagcagaacg gatcaaacga ttaatccgaa
    65701 atcaaaacaa cagtctctgt atctcacaat gcaaagcctt cgaagaagtg gtagatacac
    65761 agatgtacgg cttttcccgg caagtcggtt ttgctgtccg tataggagta atcggaaaga
    65821 acgagggcca gctgatgtta agtgagctag aacgagaatt gaatcagtta tatagtgagg
    65881 tttaccaaga aatttatgat aaaaaagaag ttggcaagga ggaatagact tgcctaagaa
    65941 agtgaaaaaa aggcatctat tagactatag catcttgatt ccctatttaa ttttatgtgt
    66001 attgggattg atcatggtat atagttctac gtcgtatcta ttattagaaa acggacaaaa
    66061 tccgtccgca tcagtcatta accagagtat tttttgggtg cttagcttga ttgctatcgc
    66121 actactttat aaaatgaaaa cagatgtttt gaaaaaccag cgtttgatca tggcagccat
    66181 cgcagttttg acgattttgc tgctgattgt tgtgtttttt ggtaaggaga tcaatggggc
    66241 gaaaggttgg ctgcaaattg caggattctc gatccaacct gccgaatacc taaaaatcat
    66301 ttctatctgg tacctgtctc tgactttatc taaaagacaa aacagtgtac aaaaagattt
    66361 tcttgggact gtgaaacgtc cacttgcaat ggttatcgga cttactgcat tagtcgctat
    66421 tcttcccgat tttggtaatg cggcagttat ctttttgatt attttggttc tgcttttagc
    66481 cagcggagtc aattatgtgt atactttgat tgtaggagta ggaggctttt gtctaagtac
    66541 ttttacgatc tggctgatca atatcacaaa cggaaagatc ttacccggac gtttacagta
    66601 tatctataat cgatttgcta tttaccaaaa tcctttctca gacgaattaa ataaagggca
    66661 tcaactagtc aacggctact atgccatgtt caacggagga ctatttggca ggggattagg
    66721 taacagtatc cagaaaaaag gatttttaca agaagcacaa accgatttta tttatgcaat
    66781 cgtagtggaa gaactgggag tgattatggg gattttgata ttagcgttgc tattctttat
    66841 gattgtgcgc attgtccttg taggtatccg ttcaaaagat ccgttcaatt cgcttttgtg
    66901 cattgggatc ggcgcaatgt ttttgatcca agtatttgtc aatcttggag gaattactgg
    66961 cgttattcct ttgacaggga tcacgttccc atttttaagt caaggaggct ccagtttgct
    67021 gatgctttcg atttgtgtcg gctttgtcct caacatcagt gcggatgaaa aacgcaagag
    67081 cttaggttta taataaaaaa agatgtagga gtggttcgat gaaaaaagtt ttagttgcta
    67141 acaggggtga aatcgctgta cgtgtatttc gtgcttgtac agaattagga attaaaactg
    67201 ttggaatcta tgcagaagaa gatgaatatt ccgtacaccg tttcaaagcg gatgaagcat
    67261 atttagtagg acaaggaaaa aaaccgattg atgcgtatct agatattgaa gggatcattt
    67321 ctatcgcgaa agaatgcgga gctgacgcta ttcatccggg ttacggtctt ttgtcggaaa
    67381 acttgaattt tgcgcaacgg tgcgaagaag aaggcatcat ttttgttgga cccaaactgc
    67441 atcatttaga tatttttgga gataaaatca aagcaaaagc tgcagctatc gaagcgggga
    67501 ttgcttcgat tccaggcaca gatggaccaa tcgcttctat agacgatgcc ttggagtttg
    67561 cgaaacaata cggttatccg attatgatca aggcagctct aggcggcggc ggacgcggca
    67621 tgcgtgtagc tcacgatgaa aagagtgcaa gagagggcta cgaacgtgcg aaaagcgaag
    67681 caaaagcagc ctttggtagt gatgaagtgt atgttgaaaa atacatcgct aatcctaagc
    67741 atatcgaagt acagattctc ggggatacgc atggaaacgt catccatctt tttgaaagag
    67801 attgttctgt tcagcgccgt caccaaaaag tagtagaagt agcgccttgt gtctctatga
    67861 atgaacagca acgtcaaaaa atctgccagg cagctgtcca attgatgaaa tatgtcgggt
    67921 atgtcaacgc aggtacagta gaatttcttg ttgaaggaga tgatttttac tttatcgaag
    67981 tcaaccctcg cgtccaagtg gaacatacga tcacagaaat gattacagat attgatatcg
    68041 taacaacgca actgttgatt gctcaaggtt tggatctgca taaagaaatc ggattgcctc
    68101 agcaagaagg aattaaatta aatggttcag ccattcaatg ccgaatcact acagaagacc
    68161 cgttgaataa ctttttacca gacacaggga aaatcgatac gtatcgttca cctggtggtt
    68221 ttggcgtacg tctggatgtc ggaaatgctt acgcgggcta tgtagtgaca ccgtattttg
    68281 attcattgct agtaaaagta tgtacgcatg gtgctacctt tgaaacagcc attcagaaaa
    68341 tggaacgttg cttgaaagaa tttagaatcc gtggagtgaa aacaaacatt ccatttatgc
    68401 tcaatgtcat cacacatcct gaattccaat caggaaatgc aaaaacaacc ttcattgata
    68461 gtacagcaac attatttgaa tttcctcgac tacgagaccg cggaaacaaa acgatgaaat
    68521 atatcggtga aattactgtg aatggttttc caggtatcga aagtggcgaa aaaccatttt
    68581 atgaagaacc acgtatgcct aaagatctaa tcacaagaac tgattacgta acggctaaga
    68641 atgtattaga tgcgaagggt gccgatgctt tggtcgaatg gatcaaagga caggaaaatt
    68701 tgttgttgac ggatacgaca tttagagacg cccatcaaag tctgctagca actcgtgttc
    68761 ggacaaagga tttcaagcag attgcacgtt tgactggaga aggtcttcct gaactctttt
    68821 caagtgagat gtggggggga gcaacatttg atgtggctta ccgctttttg aatgaagatc
    68881 cttggcaacg gttgagaaaa attcgttctt taatgcctaa tacgttgctg caaatgcttt
    68941 tcagaggttc aaatgccgtt gggtattcta attatcctga caatgtattg gtggaatttg
    69001 tcaaagaagc agcagcgcaa ggaatcgatg tatttcgaat ctttgatagt ttgaactgga
    69061 ctccacaaat ggaaaaaagt atccaagctg tacgggatac tggtaaaatc gcagaggcag
    69121 ctatttgcta tactggcgat atcaacgatc caagtcgggc aaaatacaat gttcagtatt
    69181 ataaagacat ggcaaaggaa ctagaacaac tgggtgcgca tatcattgca atcaaagata
    69241 tggctgggct gcttaaaccg caggcagctt atcgtttgat tagtgaattg aaagaaacga
    69301 cagatctgcc aatccatctg catactcatg ataccagcgg gaatggaatc atcacttatt
    69361 ctgctgcttc aaaagcagga gtagacatcg ttgacgtggc aatgagtgca atgagtggga
    69421 atactagtca gcctagcatg agcagcttgt attatgcact ggtcaatggt ccacggctgc
    69481 cagaaatcac gattgaaaat gcacagaaat taaaccatta ttgggaagat gtgcgcatgt
    69541 attacaaacc gtttgaaaac ggcttgaatg ctcctgaaac agaagtatat atgcatgaaa
    69601 tgccaggagg acagtattcg aatctacagc aacaggcaaa agctgtcgga ttaggtcatc
    69661 gctgggatga aatcaaacaa atgtatcata ctgtcaactt gatgtttggc gatatcgtta
    69721 aagtaactcc atcatctaaa gtagttggag atatggcact atttatggtc caaaatgatc
    69781 ttacagaaga agatatttat gaaaaaggcg aaacactaag tttccctgaa tctgttgtga
    69841 ctttcttcca aggagaacta gggcaacccg ttggcggatt tcctaaaaaa ttgcaaaaga
    69901 ttattcttaa aggcagacct gcattgaatg aacgtccagg tcttttggct gaatcagtcg
    69961 acttcaacga agtaaaaaaa gaattggcag aaaaaattgg ctatgaacca aaacaagacg
    70021 aagtattaag ctatttgatg tatccgcaag tctttttgga ctatcaaaaa gcatataatc
    70081 aatttggcga tgttacatta ctggacacac caacattttt ccaaggtata cgtttgggtg
    70141 aaacaatcaa tgtacaaatc gaacgcggaa aaacgcttat cattcgttta gatgaaatcg
    70201 gagaacccga tatcgaaggg aatcgcgtac tgttcttcaa tttgaatgga caaagacgtg
    70261 aaatcactat caaagataat tcgatcatca gtacggtcca aacccgattg aaagctgaac
    70321 cgacaaaccg agaacaaatt ggagcaacga tgtctggttc tgttttggat gtattagtga
    70381 aaaaaggcga taacgttaaa aaaggtgata cgttgatgat tactgaagcg atgaaaatgg
    70441 aaacggccat cgaagcacgt tttgacggag aagtcgcaca tgtatatgtg tcttcaggag
    70501 acactatttc gtctggtgac cttttgattg aagtgactga gaaataataa gcgaaaggtg
    70561 gggaaacatg aaaagactgg gtttgttttg gctgattctg ctattagtta ttgtggcatt
    70621 ctacatggaa ccagtcttgt ttccgccaaa acttgaagtg aaggaaaact ctgatcaaca
    70681 aatatcaaac caaatgacaa cgtggaaata ccaggagctt aaagcaactg gtttttctac
    70741 ttatatcgga aaaccgatcg atcagctgga aaaggatttt ggccaagcct atgatcgatt
    70801 aactagcgga tttggttttg aaacaagata ttatgaaaat aaagctagtc atctcacatt
    70861 tgaagcgaac atccaagaca gcagagtcac aactgttaaa gtactgcaaa cagggcctgc
    70921 tgatatcgcg ccgttttatt tagggatgac gatgcaagat cttacgaaaa tcactactat
    70981 ctatactaat ttcacttttg agtaccatga tacagaagtt ggaatcgaac taatggaaga
    71041 ggatatgaat tatcgtccgc taattgcttt tgataacgac acttttgcta ttttattttt
    71101 tgatcaaaca acaaacggcc tattttcagt tgtgtattta gataaagata gtcttttgaa
    71161 actattgcca tatcaagtgt ttggtgaagg ccttccacat tatcaagcag ataaatcggc
    71221 tgattgggaa tcaatcaatc gcgttaaaga acgaaaaagt acgactttat taaatatgct
    71281 tagaagagaa gacgaacttc ccatatacaa tcaaacactt gcctttcaaa accacagtca
    71341 gctgctacta catgattttt tgacaaggcc tgaggaaata ttgtcagagg aacgtatggc
    71401 tgattggcaa aaatcgctag gcagtgctca aacgacagtg aaattctcac tgacgaatga
    71461 tgaattggag aagttaatca gtaaacaaaa attagaaaat actagtgggc tatttattca
    71521 tccggcgatt gacgctactt tttcgttttt atattggtac agcgatcctt acaaccatga
    71581 ccgtttcatg gacccgggaa cggattctgt aggcattgct ttttctaaag aaaatatgtt
    71641 agtcttattg caagaagaaa acggagaaag tagtgatagc agtgatcgtt aacgaagagc
    71701 tgtttcaact ggaggatcaa tgcctccaag tcgctgaaat gataaaaaag agtaaaacga
    71761 tggcttctta tcttgaacat aaacagtcaa tggaagaatc tgcaaaagtt tgccagttga
    71821 aagaagcgtt cttgtctaag aaagaagcat ttgaaaatgt agcggcatat ggtgagtatg
    71881 cacctgatta tcgtgaaaaa aaacgagctg ttcgaaaagc aaaaagacag cttgatatgg
    71941 atgagcatgt tgcagctttc cgatatagtg aaacacagct gcaaagtctg ttggatcaaa
    72001 ttgtccaagt acttgccgaa gaagtttcta atgaaatcaa agtagatgca ggtagcccgt
    72061 tttttgaaac gaaaaaacac tttggatgcg gaggtaattg tcatgcaatt agacaatagt
    72121 gaatttgaaa taacaaaacg acgcgggctg atcgtatggg tctacagctt aaaacaatta
    72181 aagaatctga aaaagttcgg cttgttgcat tatgtctcaa gaagaatgaa atatgtcgtg
    72241 ctttacttaa atgaagagac attggaagaa acagaagaac gaatccgtaa attacatttt
    72301 gttcgaaacg tagaacgttc ttaccgaccg gatatcgaaa tgaattttgc cgaaaaaata
    72361 ggaaacaaaa cagtattaaa agacgaaggt ttcgaaattg aagagctaaa tacagaaatc
    72421 cgattagctg aacatcttta aaaatctctt atgagcagtt tttaaataaa aaataacttg
    72481 gataacgatc gatttttgat cattgtccaa gtattttttt ggtatactag taccagtacc
    72541 atttctaagg agtgagtaga tgcggataat atcaggaaat tatggcgggc gccgtttaaa
    72601 aagtttagca ggggcgaaca ccagaccaac tacagataaa gtaaaagaat ccatattcaa
    72661 tatgattgga ccatattttg atggagagac agtattggat ctgtttgcag gaagcggtgg
    72721 tttagctatc gaagcagtat ctagaggctg cagtcatgcg gtatgcgtag acaaaaatta
    72781 ccaagcgcta aaaatcatca aagaaaacat cgaaattacg aaagaaccag agaaatttac
    72841 cacaataaaa cttgatgcgg acaaagcaat cgaacggcta gcagcggaga aacaacaatt
    72901 cgactacttg tttcttgatc cgccatatgc aaaacaaaaa ataatcgatc aaatccataa
    72961 aatggaagaa ctgggacttt tttctaaaac cgcagttatc gtatgcgaaa cggataaaac
    73021 agtcgaattg ccagaaactg tggctggatt ccatcaaata agaaaacaga tttatggcat
    73081 aacggccgta actatctata gaaaagaggg aaatcaatga aacgtgcatt attcccagga
    73141 agttttgatc cttttacaaa aggtcactta gatacggtgg aacgtgcggc taaattattc
    73201 gatgaggtag taattggtgt ttttatcaat acttcaaaga aaagtttgtt tcccccagaa
    73261 gaaagaatga cattgatcac aaaagctgtt tctcatcttc ctaatgtaaa agtcatgcat
    73321 caagaaaatc aattgacggt agaaacagca aaagagattg gtgcagatgc gttgatcaga
    73381 ggcatacgaa gtatcaaaga ctttgaatat gaacgagaga ttgctcagat gaatcatcat
    73441 ctatatgatg aattggaaac tgtcttctta ttagcaaaac cagaatatag tcacgtgagt
    73501 tccagtattt taaaagaagt actgcacttt ggcggagatg tgagcagcta cttaccgcca
    73561 gtgatcaacg aagctttagc tggaaagcgt gaatcaaatg aaagctaaac aatggttgaa
    73621 aacagtactg attatcatat ttggaatgtt gatcattgga tttgtagtag ttcccttgcc
    73681 gtattatgta gaatcaccgg gagccacgat cgatctgaaa gaactaatta cggtcaatga
    73741 taaggaagat aaaggttctg ggtctttctc tcttacctct gttggtatca ggcaagctac
    73801 aggtttctta gctttaaaag ctaaaatgac cccttttgaa gaattgatca gtgaagaaga
    73861 attgactggt ggggcgacaa atgaagaata taatcaaatg caacagtatt atatggattc
    73921 ttcccaaaat gcagcaatcg aacaagcgtt aaaacttgca gacaagcctt ataaaatgga
    73981 attcaaaggc gtatatgtca tgagtgtaga gaaaacatcg aatttctttg gcaagctttc
    74041 agttggagat acagtaacaa aagtagacgg gaaaagcttt caaagtacag aagcatttat
    74101 ggattatgta aaaagccaaa aagttggaca aacaatcgaa atcagttatt tgcataatgg
    74161 tgaagaaaaa acagcttctg gtgatctgat cgagttacca acagataaaa aggcaggaat
    74221 aggtatcacg cttactgacc ataccgagat cgaatcagat acagatgttc gtattaattc
    74281 tggctctatc ggtgggccat cagcgggatt gatgttcact ttagaaatct atgaacaagt
    74341 cactggtaaa aacttgcgac atggtaagca aattgcagga actggaacga tcaacagtga
    74401 aggagaagtt ggccgaatcg gaggaatcga taaaaaagtg gcaagtgcgg ataaagctgg
    74461 cgtagagatt ttctttgcac cagatgatac gatttcagac gatgttaaga aagaatatcc
    74521 tgaaataaaa accaactacg aagaagcaaa agctgccgct aaaaaattga attcctcaat
    74581 gaaaatcgta ccagtaaaaa cagtacaaga agcgcttgac tatctagaga aaatgaaatg
    74641 aaactaaata attaaagagg ggttgtgaca cgagttctgt cacaacccct ctttttcgtc
    74701 aaaaatcttc acgataaaaa taaaaacaaa agaaaaaatt gacgtaatta gatgttgaag
    74761 ggacgatttt gccagagctc actgtttctt caattttaaa tgaaaggagt aatgaatgat
    74821 ttttactggt cattctttga aaaaagaaca atgatagaaa aaatcagcaa gaaaatacta
    74881 gcttcaccca aattgatcat ttggatgtct atgatagttc ttatcttttg tatcgcaagt
    74941 ggttttttat tatcaaattt gctattttat agagaaacag aagagactgc tgcaaaagaa
    75001 atactttctt cgtttagttc cagtggagaa gatccatcag ctgaaggaaa cgatgtagaa
    75061 gaggatgctc gtccaaaggt catgtacacc gatatcaaag gaagtgtgaa ggagcctggt
    75121 atctattcct tttcttcaga agaacgggtc tatgatgtac taaagcgtgc aggtggatta
    75181 ttagaagaag cggatagcga tcgaatcaac ttttctgcga agatagaaga tcagcaagtc
    75241 ctatatatcc ctgctgtagg tgaagagctg ccagaacatc ttgatcagtt ggcatcctcg
    75301 gaaggaaaac agcctacagc agattccgag cctcccaaaa taaatatcaa tacagccact
    75361 ccatcagaac ttcagcagat tcctgggatc ggatcagtca aagcgcaaga aatcattcga
    75421 tttagagaag aaaacggatc cttccagaaa gtggaagacc tgcaggagat ttctggtatc
    75481 ggtgaaaaga cagttgaaaa actaaaaaac ttcgttacaa ttaaatagaa tgattcaaaa
    75541 tcgactggca cgagcaataa atggacgcta atggatacta agaataatcc aattctaaga
    75601 gcttggcagg acgaggaggt agtatttttg gataggaaat agcttgattg ttcgcaaaga
    75661 aacattaaaa aaaccaatgc tgaatgtcta tctccaaaat aaaatatctg ggattcatat
    75721 tatgaatacg gctgtcagcg gcaataacag tcaagctcta cgagaacgct ttgccaaaga
    75781 tgtacttagc tatacagcag ataaagtatt tattctcatt gggacgaatg atctagcaga
    75841 gcataaacaa ctttctaaag aaacgtacca aaaaatctgc agtggatgat agttcagctg
    75901 aaaaaagact atagttctga tcatgctttc tttatcacgc caccagcagt cgatgagaca
    75961 aaacaaaaat atcggaataa caagttgatc aaagaatata tctctgttct cacagaagta
    76021 gcaaaacgcc atgattgtga agtatcgatt tttatacgga atttcttgaa caaatggatt
    76081 cgaagaattt ttataatggg ctaatggatg acggacttca tttcggcagt gaagggtagg
    76141 atcttttgtc agacaggatg attcgttata tttaaagaat attttgttta agcaatgagt
    76201 actttttaat caacttgaaa aaacggagga atataaaaca tgttagaaaa atcttcaaaa
    76261 gcatgcgata cgaataaaga aaaagttact gataagtcac ataatgaacg cattccttgg
    76321 gaccagtact ttatggctca ggcagtactg ttatctttac gtagtacgtg tacacgttta
    76381 gaagtaggag cgacactcgt tcgagaaaaa aggatcattg ctggcggcta taatggagca
    76441 gtcagtggag atgtccactg catagatgaa gggtgttacg tcgtggatgg acactgcttg
    76501 cgaacgatcc atgcagagat gaatgcactc ctacagtgtg caaaacttgg tatcccgacg
    76561 gaaggttcag aaatctatgt cacacatttt ccatgtttgg cctgtacaaa ggctttgttg
    76621 caagctggtg tgaaagggat caattatctc catgactatc gaaatgatcc ttatgcccaa
    76681 gcgttgatcg aacaattagg tgtatctgta caccaagttg ctttagactc acagcatttt
    76741 tcaaaacttc aaaatgaatt aacaggaaaa catgtctagc gttttagaaa ggcatgttcg
    76801 aaatcggttg atttttccgg caattacgtc gatgattatc tgtttgttgt attacactga
    76861 caattggggt tatcgtttgc ttcttgccgg aattttttta tggttttcgc taaaagaagg
    76921 acgttttatc gcgtacttgt gtattttgtg tagcataacg gtatccattt cttataggct
    76981 acaagagtat tcatttttag aggaaaaaac aacatctgcg caaattactg tatttacaga
    77041 tacgattcgt gcaaatggta attttttgac aatggaagga aggttatcag agaataaaca
    77101 aaagctccag ctttcttatc aagtaaacac agaagcagaa ttgaaaacat ggcttaactt
    77161 ccgaggaaag ggaatagaat tgactgctga aggcgagttc atctctccag caaaaaagcg
    77221 gaatctttat aattttgacc aagaagctta cttcaggaac catagaattt ctggacggtt
    77281 ccaaattcaa aagattgaac agatcaccat gcaaaatcac tggcgaaact ggcttcagag
    77341 aaaaagagga tcttttatct cctttgtcca atctgaattt ccgcaaaaaa caagtgtata
    77401 tattaattct ttattatggg gctataaaga tgcaggattc agagaaccag aagaaatttt
    77461 tagggagagt ggtttgctgc atctgttcag tctatcgggt ctacacatcc aattttattt
    77521 agggtggctg tattatttat ttcgacgtat cggatggcca ctacacgtca gtattattcc
    77581 gttgagtcta ctaactatct gttatgtttc tattgcagga agttctattt ctgttttgcg
    77641 tgctagtttg ctgtttttat tgcgattgct agtcaaattg ctgaataagc attattccac
    77701 catggactta tttgcagttg tcttttggct tcttttacta tttgatcctc gatttctttt
    77761 tcatgcgggt ggacagctta gttttttcat gagttttttg tttatcctga tttcttttga
    77821 tggagattgg ttgaaaaaaa cggcctctca cgtcctgttt actatgatca cgatgccgtt
    77881 gattgtatgg catttttatg agtggcctat tttaggtagt ctttttacat tactgattgc
    77941 accggtattc aagtttcttt ttcttcctgg tgtgttattt ctcacactct tcaatcgctg
    78001 gctgccggaa actttacttt tgctgataga agagggatta atcttatttg agcgaataat
    78061 cgatttttct tcagggtcgt ctctcattat aggacgtttg ccccttgtgc tatctgggat
    78121 gtatattttc ggattactga tttgtatgga taggttgaaa gaacaacctg tgaaacactt
    78181 gttttatgtt ggtctttttt cttttatttt tttgactttg ccatttttac gcttttcttc
    78241 aacgatcgct tttatcgatg tcggtcaagg agacagtatc tttttgcaaa gtccttttcg
    78301 aaaagaaacg attttgatcg atactggagg tcagttacac tttgaaagag aaaagtgggc
    78361 gcaaggtatg atacgtcctc ctgctgaaag aaatatcgtt ccatatctaa aaggtcaggg
    78421 gataagtgta atcgataaac tcatcttgac acacgacgat acggatcatg tgggagaatt
    78481 acccacgtta tctcagcatt ttactatcaa aaaaatttat atcggttggg gcgctggacg
    78541 aaatgagcca ttacaaaggc atttatctga agctcaaaaa aatggtactg agatcattga
    78601 aatcaaacat ggggacagga tcagcggtta ttatgatctt tatgtattga ctccttttga
    78661 aaagggagag ggaagaaatg aagattcgat tggtctatgg atggaatata acaataggcg
    78721 ctttttattt ttaggagatt tgaatcaagc aatggaaaaa caattgcttt tgatttatcc
    78781 taatctgaaa gccgatgtag tcaaattagg tcatcacggt agccgtacgt caagtaacac
    78841 agattttctt tcccatattg aagcaaaaca tgggattata tcctgcggcg taaataatcg
    78901 ttatggtcac ccgcacagcg aagtattgga gacactagaa gaaaatcaga taaaaacatg
    78961 gcgaacggat caacaaggaa tgatcaccta tcgctggcat ccagtttttc atcctcatgg
    79021 gctagtggaa acaatgatag actagtaatt ctcatctgct catggtagga taatttttga
    79081 aagtggtgag taatttgagt atacaagcag agttacaaaa aatacgtaaa gaaaaacttg
    79141 catcttgtta cttgatttta ggaacagaaa agtttctcca agatcaagtt cggacagaaa
    79201 ttgtcaaaaa aatccaatta gagggcgaag atgacttaaa ttttttaagt tttgacatgg
    79261 aaaatgcaag tttagatgag gtagtagctg aagcagagac gttgccgttt ttcggagatc
    79321 aacggttagt attcgtagag aatccttatt ttttaacagg tgaaaaagta aacaatggaa
    79381 tcgaacaaaa tactgattta ttgacggctt atttgaaaga accattagaa tcgactgtat
    79441 tagtattctt tgcgccatat gaaaagttgg atgaacggaa aaaagtaacg aaacaattga
    79501 aaaaaacagc aattatcatc aatgtccaac agttgaatga aaaagaagta cgtcaatatc
    79561 tgatgaatac cttagagaat tcctcaatca aaatggaacg aagtgcaatt gatttattct
    79621 tgaggttgac cgacttagat ttatccaaaa tgatgagaga gttacagaaa ttggtccttt
    79681 atggacaaaa tcaacagcaa atcacggtaa gagaagtaga agaattagtt cctaaaacgt
    79741 tagaacataa tatttttgac atgacccaat atattttaaa agggaagacg gaacaagctt
    79801 tgcgtttata cgaagattta gttatgcaag gggaagaaac aatcaaaatt aatgcgattt
    79861 tgctttctca attacgttta tttttacaga caaaatttct tgtaaagatt ggttatcagc
    79921 aggcaaatat tgctgaaaca ttgaaaatcc atccatatcg tgtaaaactt gccatgcagg
    79981 aagtaaaaaa attcgatgaa catttgctgg tacagttgtt tgatcaatta gttgagatgg
    80041 attatcaaat caaaacggga caaatcgaaa aagaattaag ttttcaacta ttcgttttgc
    80101 gcacagggca gttgttaaga aatcgataag aagaaagacg agggcaaagc atgcgggaag
    80161 gtcgtttgtt tttgtccttt ttttgacaaa taaaaaaatc ggccgaagcc gattctttta
    80221 tttagcgatt tttttgctta aacgagattt gtcgcggtta gctttgtttt tgtggattag
    80281 accttttgat tcagccatat caatagcttt aacagcttct ttatataacg catctacgtt
    80341 gtcagcacct gaagctacag catcttcaaa ttttttgatt gcagtacgca tagcgcttgt
    80401 ttgagatgaa tttttcacat tagcgttttc gcttgtacga acacgtttga ttgcagattc
    80461 aatgtttggc attagtttca cctccgataa cttaagttct tacgcgtgtt accgcataat
    80521 tcaacattca ttattatacc taaaggcagc gtgctttgca atatgaacat gacaagtttt
    80581 tttccttaac agtcttttat taggattttt tttcagtaaa aacggatagt acaagctgaa
    80641 aaaaatcaaa aagacgctat tgtgggaggc aatagcgtca aaccaaacta gatttttatt
    80701 tatctattta aacaaagttt gaatgggaat gtttggtact aagttattat ataacaaaga
    80761 gaaccgtttg caaagttttt tttgaaaagg gatacgattc cctgttgttc tttgtttttc
    80821 tattaagaaa ctgcagttat tttataacca gtatcctttg atcagtaaag gctattctgg
    80881 tgtaccgatt attttaatgt attcatcgat attttttatc aacatttttt ttatgaccaa
    80941 ctggttccga cggttccatt tgccacttac ttccagcaca tatttattta ggggcaaacg
    81001 taagatatat tttgccaaat agcgatcttt aagcaggcca ttgatcacac ggtcctcaag
    81061 ctgcaagttg atcccagtca gcatcattgg ctgttcttgt aaaacgtacg tatcaattaa
    81121 aagaccttca aaagtttcca caagcgtctt cctttctttt caaagtaacc tgaaaacagg
    81181 cactaattct tccattttga tattcgttga agtggatgcg atcattcttt cttttgtcca
    81241 ctgtaatcta gtataacaaa aaagagcaga agagccaaaa agaaaaaaga gcatcgaatt
    81301 ctataggaag tctcattggc acggcctaaa ttattacaaa acattgatta aaaaaagccc
    81361 ctgcatcgca ggagcatccc ctatatcgtt ttaataggtc aatcaaaaag cagtatcaaa
    81421 cacattttgt atcaaatcag gaaataatag ataagctaag cccagaatca aaagtaccgc
    81481 tagaagaaac cagccgattg ttcctatgaa atactttaat ttttcgaaca ttgtatcacc
    81541 tcgttgtcag tatatcacgg atcagtaaat tgggggtgtt aagaatctcc taaaatagac
    81601 aaaatttgtt ctattttcct aaagcaaata cttgtaagat acagtaagtg ataccagaag
    81661 caatgaccgg tccggcggcg attcctctta agaagacaac acctaaaatc gtgccgaaaa
    81721 caagtgcaac agtgatttgt ggatcagaag caatcaatcc tacacccttg gaagataaga
    81781 cagccactag cccaccgcaa aaaacagcta cccagccaat aggggattta aaggcttcta
    81841 atagatcacg tagaccgatt tctcctgttg cgatagggat tagaatagcc gctgtgatga
    81901 tcgtcacccc aatattgatt cctttgcctt gaagccaagg gaaaaaggaa cgggaggctg
    81961 gtatgagctt cataatcaaa acaaaagctg ccgctatcat cagactttga tttttagcta
    82021 aaattgctac aagtaaaata attcctaaaa aaagccaact ttccatgaaa tatattcctc
    82081 actttcttcg aaacaatcat aacatgtttt tagaaagtta caaaagtagg aattcatcct
    82141 tcaagaccta ttcataagct gtttttttca taaaataaac aaaatcataa aaaaacactt
    82201 tgcttatttg acagaagaca gccgcgcttt gtatagtgga caagacttac atttttttga
    82261 agaatcagaa agatcaggta aaggtcttag taaacgcaag ccattcagaa taaccaatat
    82321 tgtgcttcct tcgtgtccta caactccaag aggtagattt aaaaattgca ggaagttaga
    82381 agcaatcaac aagagaataa caagaatcga aaaaataata ttttgtttga tgatccgccg
    82441 catttttaaa gatagccgat gactgtagac aaatttattt aagtcgcttt tcattaagac
    82501 gatgtctgcg atatccatag cggcatctgt cccttgcccc atagcgacac ctaaagaagc
    82561 ggctgctaaa gcaggcgcat cattgactcc atcaccaacc atcgcattta cttggtaaag
    82621 ttcattctct tttttgacta gttgtgtttt ttcttccgga gtacagcttg catagtaatg
    82681 ttcaatccct acttgttcag caatggcctt tgctgtgcca gaatggtcgc cggttatcat
    82741 acttgtatgg atgttttggg atttaaagta ggaaattact tgttgggtat ttgcttttgg
    82801 aatatctagc agacctaaaa cagcaattaa ctgctgatct ttagaaagat aaatcacggt
    82861 tttgccttga ttttccaaac gttcgatcat ttccgcaatc tctttgctga ttatcatcgt
    82921 ctcaggatca tatgcgtgct tgccaacttt ccattgcgca cctaaatagg tagtttgtaa
    82981 tccaaaacca gtaatttctt ctattggtaa gtattggatt tcttcaggta ttgtgcaatc
    83041 gaaatattgc aaaatcgcat gagcaagtgg atgcgtcgtt ttttgttcca tagcaacaag
    83101 aagttgttgt gccaaatcct tttcatcaaa gaaaaagctg tcagttacaa ctggttttcc
    83161 tttagtgagc gttcctgttt tatcaaaggc aatcgctttt aattcagaaa gcttttctaa
    83221 atggataccg cctttgatta aaatcccatt ttttgcagaa tttgaaagag cagctagaga
    83281 agctggtgta gcagatgcga caagcgcaca aggagaagcc acaactaata ataccattcc
    83341 tcgataaaag ctttcatccc aactccaacc taggaaaaag tgggggagaa ggatcattag
    83401 aggaacagca agcaaaatca attttacata attattttcg attcgttcga taaatgaagc
    83461 tgtttgagtt ggcgtattct gcgcttcttc tactaattgg ataatcttag caaagacagt
    83521 ttcggaactc gtttttgtta ctcgaatcgt gatgggattt ccttgattca gcgttcctgc
    83581 atacatctca tcgcctaaat atttttctac aggcacagat tcaccgctta atgcagactc
    83641 atcgatcgtt gcctgtgtgc tttctaaaaa gccatctatc ggaacagctg ctcctttagc
    83701 gacaaatacc aagttatcca cctttagttg agaaacatct acttcttgca tttgtccatt
    83761 ttcttttaat aattgggctt tttgtggttg aagattcatc aaagcagtaa tttctttttg
    83821 acttttatta gttgtgtatt cctctaatgc accgcttaaa caaaagatga atgtcaacat
    83881 tgctccttca aagtaattac cgatcaaaca ggcaccaata gcagctaaag ccatcaataa
    83941 atccacattc agtgtccgat cattccacaa ttctttcaag ccttccgatg tttgtttata
    84001 tccaccgatg ataatggcac tgatataaaa aagtggggtg atggtaagag agaatcggtc
    84061 gaagataaaa ccgaagatca taaataaaag gcagccgatt gttgctgcaa aagctttttt
    84121 ctgattagtc atcgttatta gacctccttg tcaaaacaag gatagcataa taaaaatgta
    84181 attgataata ataacagcta aatgagaacg ataatggtta tcatttggtg attaagcttg
    84241 atgttgtttt tattatttat aatgattatt attaaagaaa ataaaaaacc agaacatttc
    84301 tcaaataaga
//
LOCUS       DACDTH010000005        82889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDTH010000005 DACDTH010000000
VERSION     DACDTH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.005"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            24..413
                     /locus_tag="IWK54_000371"
     CDS             24..413
                     /locus_tag="IWK54_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HAP9592523.1"
                     /translation="MSPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIA
                     VENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTI
                     AFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            541..1224
                     /locus_tag="IWK54_000372"
     CDS             541..1224
                     /locus_tag="IWK54_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9592524.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1533..1606
                     /locus_tag="IWK54_000373"
     tRNA            1533..1606
                     /locus_tag="IWK54_000373"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1567..1569,aa:Arg,seq:tct)
     gene            1765..2124
                     /locus_tag="IWK54_000374"
     CDS             1765..2124
                     /locus_tag="IWK54_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9592525.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2247..3878
                     /locus_tag="IWK54_000375"
     CDS             2247..3878
                     /locus_tag="IWK54_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9592526.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3940..4581)
                     /locus_tag="IWK54_000376"
     CDS             complement(3940..4581)
                     /locus_tag="IWK54_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9592527.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            4863..5171
                     /gene="rplU"
                     /locus_tag="IWK54_000377"
     CDS             4863..5171
                     /gene="rplU"
                     /locus_tag="IWK54_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9592528.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5198..5542
                     /locus_tag="IWK54_000378"
     CDS             5198..5542
                     /locus_tag="IWK54_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9592529.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5555..5848
                     /gene="rpmA"
                     /locus_tag="IWK54_000379"
     CDS             5555..5848
                     /gene="rpmA"
                     /locus_tag="IWK54_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9592530.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6255..9089
                     /locus_tag="IWK54_000380"
     CDS             6255..9089
                     /locus_tag="IWK54_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9592531.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            9280..9771
                     /locus_tag="IWK54_000381"
     CDS             9280..9771
                     /locus_tag="IWK54_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9592532.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9874..10863
                     /gene="manX"
                     /locus_tag="IWK54_000382"
     CDS             9874..10863
                     /gene="manX"
                     /locus_tag="IWK54_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9592533.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10896..11699
                     /locus_tag="IWK54_000383"
     CDS             10896..11699
                     /locus_tag="IWK54_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9592534.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11720..12634
                     /locus_tag="IWK54_000384"
     CDS             11720..12634
                     /locus_tag="IWK54_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9592535.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12717..13091
                     /locus_tag="IWK54_000385"
     CDS             12717..13091
                     /locus_tag="IWK54_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9592536.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13230..13475
                     /locus_tag="IWK54_000386"
     CDS             13230..13475
                     /locus_tag="IWK54_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9592537.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13647..14711
                     /locus_tag="IWK54_000387"
     CDS             13647..14711
                     /locus_tag="IWK54_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9592538.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            14957..15382
                     /locus_tag="IWK54_000388"
     CDS             14957..15382
                     /locus_tag="IWK54_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9592539.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15375..15824
                     /gene="nusB"
                     /locus_tag="IWK54_000389"
     CDS             15375..15824
                     /gene="nusB"
                     /locus_tag="IWK54_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9592540.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            15921..16775
                     /locus_tag="IWK54_000390"
     CDS             15921..16775
                     /locus_tag="IWK54_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9592541.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16776..18113
                     /locus_tag="IWK54_000391"
     CDS             16776..18113
                     /locus_tag="IWK54_000391"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9592542.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18118..18342
                     /locus_tag="IWK54_000392"
     CDS             18118..18342
                     /locus_tag="IWK54_000392"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9592543.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18344..19234
                     /locus_tag="IWK54_000393"
     CDS             18344..19234
                     /locus_tag="IWK54_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9592544.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19243..20061
                     /locus_tag="IWK54_000394"
     CDS             19243..20061
                     /locus_tag="IWK54_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9592545.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20168..20617
                     /locus_tag="IWK54_000395"
     CDS             20168..20617
                     /locus_tag="IWK54_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9592546.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20635..22317
                     /gene="recN"
                     /locus_tag="IWK54_000396"
     CDS             20635..22317
                     /gene="recN"
                     /locus_tag="IWK54_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9592547.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22372..22488)
                     /locus_tag="IWK54_000397"
     CDS             complement(22372..22488)
                     /locus_tag="IWK54_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9592548.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22561..23517)
                     /locus_tag="IWK54_000398"
     CDS             complement(22561..23517)
                     /locus_tag="IWK54_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9592549.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23688..24056
                     /locus_tag="IWK54_000399"
     CDS             23688..24056
                     /locus_tag="IWK54_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9592550.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24226..24657
                     /gene="mraZ"
                     /locus_tag="IWK54_000400"
     CDS             24226..24657
                     /gene="mraZ"
                     /locus_tag="IWK54_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9592551.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24678..25637
                     /gene="rsmH"
                     /locus_tag="IWK54_000401"
     CDS             24678..25637
                     /gene="rsmH"
                     /locus_tag="IWK54_000401"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9592552.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25642..26043
                     /gene="ftsL"
                     /locus_tag="IWK54_000402"
     CDS             25642..26043
                     /gene="ftsL"
                     /locus_tag="IWK54_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9592553.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26044..28236
                     /locus_tag="IWK54_000403"
     CDS             26044..28236
                     /locus_tag="IWK54_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9592554.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28262..29224
                     /locus_tag="IWK54_000404"
     CDS             28262..29224
                     /locus_tag="IWK54_000404"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9592555.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29238..30599
                     /locus_tag="IWK54_000405"
     CDS             29238..30599
                     /locus_tag="IWK54_000405"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9592556.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30625..31713
                     /gene="murG"
                     /locus_tag="IWK54_000406"
     CDS             30625..31713
                     /gene="murG"
                     /locus_tag="IWK54_000406"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9592557.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31728..32948
                     /locus_tag="IWK54_000407"
     CDS             31728..32948
                     /locus_tag="IWK54_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9592558.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33104..34429
                     /gene="ftsA"
                     /locus_tag="IWK54_000408"
     CDS             33104..34429
                     /gene="ftsA"
                     /locus_tag="IWK54_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9592559.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34452..35693
                     /gene="ftsZ"
                     /locus_tag="IWK54_000409"
     CDS             34452..35693
                     /gene="ftsZ"
                     /locus_tag="IWK54_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9592560.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35710..36387
                     /locus_tag="IWK54_000410"
     CDS             35710..36387
                     /locus_tag="IWK54_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9592561.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36401..37000
                     /locus_tag="IWK54_000411"
     CDS             36401..37000
                     /locus_tag="IWK54_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9592562.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37015..37302
                     /locus_tag="IWK54_000412"
     CDS             37015..37302
                     /locus_tag="IWK54_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9592563.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37351..38133
                     /locus_tag="IWK54_000413"
     CDS             37351..38133
                     /locus_tag="IWK54_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9592564.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38154..38861
                     /locus_tag="IWK54_000414"
     CDS             38154..38861
                     /locus_tag="IWK54_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9592565.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39123..41921
                     /gene="ileS"
                     /locus_tag="IWK54_000415"
     CDS             39123..41921
                     /gene="ileS"
                     /locus_tag="IWK54_000415"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9592566.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(41970..43496)
                     /locus_tag="IWK54_000416"
     CDS             complement(41970..43496)
                     /locus_tag="IWK54_000416"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9592567.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43511..44158)
                     /locus_tag="IWK54_000417"
     CDS             complement(43511..44158)
                     /locus_tag="IWK54_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9592568.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44342..44671)
                     /locus_tag="IWK54_000418"
     CDS             complement(44342..44671)
                     /locus_tag="IWK54_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9592569.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44848..45576
                     /gene="sfsA"
                     /locus_tag="IWK54_000419"
     CDS             44848..45576
                     /gene="sfsA"
                     /locus_tag="IWK54_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9592570.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45592..46605
                     /locus_tag="IWK54_000420"
     CDS             45592..46605
                     /locus_tag="IWK54_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9592571.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46605..47882
                     /locus_tag="IWK54_000421"
     CDS             46605..47882
                     /locus_tag="IWK54_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9592572.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            47945..50647
                     /locus_tag="IWK54_000422"
     CDS             47945..50647
                     /locus_tag="IWK54_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9592573.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50799..51116
                     /locus_tag="IWK54_000423"
     CDS             50799..51116
                     /locus_tag="IWK54_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9592574.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51146..51466
                     /locus_tag="IWK54_000424"
     CDS             51146..51466
                     /locus_tag="IWK54_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9592575.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51574..53034
                     /locus_tag="IWK54_000425"
     CDS             51574..53034
                     /locus_tag="IWK54_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9592576.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53102..53323
                     /locus_tag="IWK54_000426"
     CDS             53102..53323
                     /locus_tag="IWK54_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592577.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53354..53536
                     /locus_tag="IWK54_000427"
     CDS             53354..53536
                     /locus_tag="IWK54_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9592578.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53536..53949
                     /locus_tag="IWK54_000428"
     CDS             53536..53949
                     /locus_tag="IWK54_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9592579.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54072..55253
                     /locus_tag="IWK54_000429"
     CDS             54072..55253
                     /locus_tag="IWK54_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9592580.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            55642..55715
                     /locus_tag="IWK54_000430"
     tRNA            55642..55715
                     /locus_tag="IWK54_000430"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55676..55678,aa:Arg,seq:cct)
     gene            complement(55752..55952)
                     /locus_tag="IWK54_000431"
     CDS             complement(55752..55952)
                     /locus_tag="IWK54_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9592581.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(56257..57489)
                     /locus_tag="IWK54_000432"
     CDS             complement(56257..57489)
                     /locus_tag="IWK54_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9592582.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57743..58312
                     /locus_tag="IWK54_000433"
     CDS             57743..58312
                     /locus_tag="IWK54_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9592583.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58490..58930)
                     /locus_tag="IWK54_000434"
     CDS             complement(58490..58930)
                     /locus_tag="IWK54_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9592584.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59088..59852
                     /gene="map"
                     /locus_tag="IWK54_000435"
     CDS             59088..59852
                     /gene="map"
                     /locus_tag="IWK54_000435"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9592585.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            59884..60807
                     /locus_tag="IWK54_000436"
     CDS             59884..60807
                     /locus_tag="IWK54_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9592586.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            60883..62022
                     /locus_tag="IWK54_000437"
     CDS             60883..62022
                     /locus_tag="IWK54_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9592587.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62015..62815
                     /locus_tag="IWK54_000438"
     CDS             62015..62815
                     /locus_tag="IWK54_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9592588.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            62815..63642
                     /locus_tag="IWK54_000439"
     CDS             62815..63642
                     /locus_tag="IWK54_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9592589.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63620..64354
                     /locus_tag="IWK54_000440"
     CDS             63620..64354
                     /locus_tag="IWK54_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9592590.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            64454..65320
                     /gene="rfbA"
                     /locus_tag="IWK54_000441"
     CDS             64454..65320
                     /gene="rfbA"
                     /locus_tag="IWK54_000441"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9592591.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65334..65906
                     /gene="rfbC"
                     /locus_tag="IWK54_000442"
     CDS             65334..65906
                     /gene="rfbC"
                     /locus_tag="IWK54_000442"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9592592.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            65928..66956
                     /gene="rfbB"
                     /locus_tag="IWK54_000443"
     CDS             65928..66956
                     /gene="rfbB"
                     /locus_tag="IWK54_000443"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9592593.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67054..67905
                     /gene="rfbD"
                     /locus_tag="IWK54_000444"
     CDS             67054..67905
                     /gene="rfbD"
                     /locus_tag="IWK54_000444"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9592594.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            67940..69973
                     /locus_tag="IWK54_000445"
     CDS             67940..69973
                     /locus_tag="IWK54_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592595.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70017..71297
                     /locus_tag="IWK54_000446"
     CDS             70017..71297
                     /locus_tag="IWK54_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9592596.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71507..72313
                     /locus_tag="IWK54_000447"
     CDS             71507..72313
                     /locus_tag="IWK54_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9592597.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72325..73545
                     /locus_tag="IWK54_000448"
     CDS             72325..73545
                     /locus_tag="IWK54_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9592598.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73535..75121
                     /locus_tag="IWK54_000449"
     CDS             73535..75121
                     /locus_tag="IWK54_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9592599.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75160..77298
                     /locus_tag="IWK54_000450"
     CDS             75160..77298
                     /locus_tag="IWK54_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9592600.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRSD"
     gene            complement(77666..78649)
                     /locus_tag="IWK54_000451"
     CDS             complement(77666..78649)
                     /locus_tag="IWK54_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP9592601.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            79027..80418
                     /locus_tag="IWK54_000452"
     CDS             79027..80418
                     /locus_tag="IWK54_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9592602.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            80434..81474
                     /locus_tag="IWK54_000453"
     CDS             80434..81474
                     /locus_tag="IWK54_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9592603.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            81493..82578
                     /locus_tag="IWK54_000454"
     CDS             81493..82578
                     /locus_tag="IWK54_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9592604.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            82611..>82889
                     /locus_tag="IWK54_000455"
     CDS             82611..>82889
                     /locus_tag="IWK54_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9592605.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLL"
BASE COUNT        27125 a        13937 c        17545 g        24282 t
ORIGIN      
        1 cttatattat tgaggacaaa gaaatgagtc catattatgc acaaggaaat ctgatatatg
       61 tacgaccgaa aaatacaaat gagattttag tcggagatat tattacatat tatgaaaatt
      121 caggaaaaaa aatctcgacg cgtcgcgtaa tagccgttga aaacaaccac gaagattttt
      181 atacaaaagc tgatacaaag acttatattg agcctggagt agtaaaaaag agaaatatta
      241 tcggtagtcc catctttcag attccatata ttggactagt cctttcaaaa acggcatttg
      301 catggattca ggggttattc tttacgattg ctttttgcct aacaggaatc acagcatgga
      361 atacctttgt agagttgaaa aagaagcgga gacgaacaca gaaatttttg taagaatgtt
      421 aaaaacataa atgtattcca gagatccaac gactcttctg aacatgaaac acgagaaaac
      481 actcatgcag cttgtagatt tagtcagttg ttcgtataat gtgtgaagag gaggagtagt
      541 atgcgcaatg aaatgatgca tcgtcctgta gtcaaagaag aactattgga ttttatgcgt
      601 acccagcaaa aaaaattacc gggtgagcta ggcaaactgg aagaagaagc tcatgtagca
      661 gaagtaccag ttattcccca tgaaacagtt gtatttttga aatttttact tggacagctt
      721 cagcctgaac gaatcttgga gatcggagca gctatcggtt tttcatcaag tgtcatggcc
      781 acgacaatcg gagaaaacgc tcatgttacg acaattgatc gtttcgatgt catgatcgag
      841 aaagctaaaa aaaactatga acgtctagga ctgacagata aagtgacttt gcttgaaggt
      901 caagcagcag atatcttgcc ggagttgtct ggtccgtatg actttatttt tatggatagt
      961 gcaaaatcaa aatatattga atttttacct gaatgcttac gtttgttaag aaaaggtggc
     1021 gttttgatgg tagatgacat cttccaagga ggaacgatct tgctcccaga tgaagagatt
     1081 ccccgtggca aacgagctat ccatcgtaaa ttaaatgaat ttcttcgtgt tgtgatggat
     1141 catcccgatt tgacatcgac aatcttgcca ttaggtgatg gtgtgatctt aatgacaaag
     1201 gaatcagaaa caatcgagtt gtgatacatg attatggcag cctgtagata atcgattgaa
     1261 tcgagggctc aattcgaact tgatgtaaaa gtttctatat ttgtaagtca attaaaggat
     1321 acaggaaatc taaataaaca ggagtagcat gaagtcaatg tagctcctgt tttcaattga
     1381 taaccttaga acagctgaag gagaatgtct cgttttaaaa gattgattta gtagttgggc
     1441 caaaataatt cattatttgc taattaaata actatttcgt attgacagaa gcaatttaat
     1501 tatctataat tattgatgtt ccgctgattc ttgtcatagt agctcagctg gatagagcat
     1561 ccgccttcta agcggacggt cgggggttcg aatccctcct gtgacgctaa aaaaccgtta
     1621 gaaactgagg tttctaacgg tttttctttg ttttttgtgc caatattgtt tgttttcaaa
     1681 ggcaaataaa ataaaaaaac atccttatcg agtacaatta taaggagcaa aaaaataaaa
     1741 ctcatcagaa aagaggaaga agatatgacg atactatcta gaattttagt gactctagtt
     1801 gcaattgaat ttttttatat catgtatatc gaaacggtga gaactgattc tgacacgaca
     1861 agtagggttt ttaaaatgag taaagaagag ttaagcagaa aatctgttca aacattgttt
     1921 aagaaccaag gagtgtacaa tggactatta ggagtgggac tactttatgg agcttatctt
     1981 tcttccgcat ctaaagaaat cacaagtatg ctattaatat ctattttttt tgttgcatta
     2041 tatggcagtt tgactagcga tcgttggatt ttactcaaac aaggtgggct agctctgttg
     2101 gcattgcttt ctattttctt ataaatatag aaaaaataag aatttatcta acaggaaact
     2161 ttgtgaaaaa tattacttta tctcatgaaa tatgatattc taatgaatgg tattttagaa
     2221 aatttcatga aaaaggagtt agataggtgt tcaaaaatta tgtttcctca ctaaaaaaag
     2281 aattttcagg ttatgatctt tcaagatttc agaaagatct tttggctggg atcacagtag
     2341 ctgctgtagc attgccttta gccttggctt ttggggtatc aagcggagct gatgcggccg
     2401 caggtttgat tacggctatc attgccggta tcgttattgg cggtttatca ggcggttttt
     2461 atcaaatatc aggtccgaca ggtgcgatgg cagctatctt gatgtctatt gcggcaacaa
     2521 agggaatgca aggtgtattg cttgctactt ttttagcggg tctattttta cttgcagcag
     2581 gacttttccg attaggaaca ctgacttctt ttatcccagc acccgtaatt acggggttta
     2641 cttcaggtat tgctatcatt attgcattag gtcagatcga taatttattt ggtgttcact
     2701 cggaaggtac aaacgttatt gaaaaactaa gtagttatca aacgatagga tttccgataa
     2761 acacagcctc tttgatcatg ggagggctag tcattttagg gatgtttatc tatccaaaaa
     2821 aatgggcgaa acgaatgcct agttcgctgc tggctatcat tttggcaaca gcagttatgc
     2881 tattgttcca tttaccagta gcaactgtgg gtaagattcc acaaacattg atcagtagta
     2941 atcgtttgaa tatgggagat tttagctttt ctgccttgca acaagtggca gttccagcta
     3001 ttagcattgc tttattagga atgattgaaa gcttgctatg tggtgcttcg gctggccgaa
     3061 tggcaaataa gcccttagat agcaatcaag aattaatggc gcaaggaatt ggaaatctgt
     3121 tgcttccttt ctttggcggg attcctgcta ctgcggccat cgcccgtaca agcgtggcca
     3181 tcaaatctgg tgcacaaact agattagctg gtatgataca tgcagttgtc ttactgctct
     3241 ccatgcttgt ttttgcgcca gtgatgtcac agattccaat gccagcactt gcaggtgttt
     3301 tgattgtgac ggcttggcgt atgaatgaat gggaaacgat caaggagcta tttactaata
     3361 aatattggtc agcagttcta ttgtttatcc ttacgatgat ttgtacagtt atctttgatt
     3421 taagtattgc tatcgtgatc ggggtgatcg gcggctgtgt attttttgtt gtcaaaagcg
     3481 ctgctatcac gatttcatta gaaacgatcg attggcaaag aatggggctg ccagaaacgg
     3541 aaaagctgaa taactggaca gtagtgtata ttagtggtcc actgtttttt atgtcttcag
     3601 aaaaactcaa aaataaattg gaagaactaa atcatcacga tggtgtcatt ttctccatgc
     3661 gaggcgtacc aagtattgat ttgacggcac gtgcagtttt tgatgaattt caagaaaaag
     3721 cagagcaaag aggacaaacg attatctata cttccttgca gccagaagta gaaaaacaac
     3781 tttctcactt gtgggaacat acattcacgc atcaacatca aacagttgct catgcgttga
     3841 cagctttaca tgcgaaatat actgcaatta gccattaaaa caatgaaaaa gaacagacaa
     3901 gactgtaaac tgaagatttt atggtcttgt ctgttctttt taatattgct gtaaaagtga
     3961 agctagtact tgccagtctt gatggataac aggtacaagg gagcgattgt agaaaatagc
     4021 tgctggatga aaaagtggaa gaattcgata gtttttttct gaccattcgt aggcatcctt
     4081 ttgttctgtt aattgtaaga caggccctgt atagagttga ccatgatttt tggtaaccgt
     4141 atattctttg ccaagtaaac gttgaagacc aatgtttcca agtgtcgcaa ttaattttgg
     4201 ctggaccttc atcagttcgt aatccaatat tggtgcatgc gcaaatactt cggaacgagt
     4261 aggtgtccga tttggataaa cagtttctga ctgttgtgtc cgtttattga tccgatgagt
     4321 cacgcgatac gggcggctcc ttacagcact ggtgatatac acctcttctc gtgttaagcc
     4381 agttgaagaa agtgcctgca tcaattcttt tccagcttga ccgctaaaag gaacatgcgt
     4441 atcaatctct gttttccctg gggcttctcc cacgagcatc agtttaggat gaagaggtcc
     4501 ttgcccagcg ataaatcctt ctacaggacg gccgttcatt cttaaagaag cttcttgtat
     4561 cagttctttt gggtattcca tttatgctcc tcctttttct tttattataa gaataaaaga
     4621 aacacaatgc gactatttta tttggtagaa ctgtgaggat agaaaagcag aagtattgct
     4681 tgaatttttg ttttactttt tgattttaaa tttagttgac taacggttgc tgttctggta
     4741 agctagtact gttgtaattg tggactccac agctacaacc gctcagaaca agtaataagc
     4801 acagcatatg ctgtcacttc ggatggcgag tctaagtata tcagaaggag gtgctttata
     4861 gcatgtacgc aattattaaa actggtggta aacaagtaaa agttgaagtg ggacaagcaa
     4921 tctatgttga aaaacttgac gttgaagcag gagaaaaagt tgtttttgac gaagttatct
     4981 tagtaggcgg agaatctaca aaagtaggcg ctccaactgt tgcaggtgca actgttgaag
     5041 gtactgttga aaaacacggc aaacaaaaga aagtcgttac tttcaaatac aaacctaaaa
     5101 aacattcaca ccgtaaacaa ggtcatcgtc aaccatatac aaaagtggtt atcgacgcga
     5161 tcaacgctta atagcgtttg agaaagaagg cctatatatg atcaaaggaa catttaagcg
     5221 aaatgacgca ggtcagatcg tttcattcac tttgaccggg catgctgatg ctggtcctta
     5281 tggaagtgat atcgtttgtg cgggtgtttc tgcgttagcg atcagcactg tcaacgggat
     5341 tgcctcatta gccggttttg agccgattgt tgaaatgaat gaagaagaag gcggttatct
     5401 ttataccgaa gtcacttcag gaatgacaca agaacaaaac aatattgctg agattctatt
     5461 agaaaatctt ttattaggat tacaatcgat agaagctgaa aactcagaat atatccaaat
     5521 taaaacaatt aatgataaat aggaggtgca gaccatgttg ttatctatga atctacaatt
     5581 atttgcccac aaaaaaggtg gcggttctac atctaacggc cgtgactcag aatcaaaacg
     5641 tctaggtgct aaacgtgctg atggacaaac agttacaggt ggatcaattc tttaccgtca
     5701 acgcggtaca aaaatctatc caggtgtaaa cgtgggtatc ggtggagacg acactttatt
     5761 tgcaaaagtt gatggcgtag tacgtttcga acgtaaaggc cgtaacaaaa aacaagtgtc
     5821 tgtttatcca gtagctcaag aagcttaatt gaatcttatg attaaagagg ttgtgacaag
     5881 acttttgtca cagccttttt tgtttataaa aataaaagtt gtagtggagg ctacgaagtt
     5941 tactttgtct ttttacactt aatatttttt tgccaatttg gaatcaggat gttttgataa
     6001 ctatcgtttt caattgatgg aagctatatt gtttgctatc agtgaaataa ttatatggta
     6061 aacagtgttc tttttcttgt ttccattata aaaatcgtat cgggattttt cgttctttta
     6121 aattgaacat ttctatctat ccgcgtgtta tcaacaaaac aaaatcaggt aaatagttga
     6181 cgaaacagaa ataaatacat agtattatgt tttgaagcct aagtacaata gtctatagaa
     6241 aaggagcgat accattggca aaacgcattg accgaattta tatgtatgtc aaagaaaaaa
     6301 ccgctcattt aacaaaggca gaatatgatc aaggagtaac aacacaggaa attgctgaag
     6361 tgttagggat tcagcgaaca aatagcagta aagatctgaa tcagctagtg cgcgaaggaa
     6421 aactgataaa gacagacgga cgaccagtac gttacatata tcaaacactc gttacgcacg
     6481 taaagccagt tacaaaacat gtagtcagtt acaaagaaac gccggtctct gaagaaaaaa
     6541 atacactcca aatagatact aaggatatct ttgcaaaaat cattggagct aacggcagca
     6601 tgaaaaattc agtggaacag gcaaaagctg cgattttgta tccgccaaga ggattaaatt
     6661 gtttgattac aggacctacc ggatcaggaa agacttattt tgcacacgcg atgtatcatt
     6721 ttgcaaaagc gcatcatgta atagatgagg aaacggaact gattgttttc aattgtgctg
     6781 attatgccaa taaccctgag cttttgatga gtcatttatt tgggtacgca aaaggtgctt
     6841 ttacaggcgc agatgaagaa aaaatgggga ttatcgatca agcggatggc ggtatgctgt
     6901 ttttggatga gattcaccgg ttgccgcctg aaggtcaaga aatgatcttt tactttatgg
     6961 atcatggaac ttacagtcgt cttggggaaa caacgaaatc ccatgaagca aatgtacgga
     7021 tcgtaggtgc gaccacagaa gacccaggtt cttctctatt agaaactttt gttcggcgta
     7081 tcccaatcaa tatcaaactt ccggcttttg aaaaacgtcc tgcaaatgag aaagtcgatt
     7141 tagtaaaaat catgattgcc cacgaagcaa accgcacgca gcgaaagatt tctttgacag
     7201 aagatgtagc aaaagcattg atcggcagtg tgacctatgg aaatatagga caattgaagt
     7261 caaatatcca gttagtatgt gcacgaggat ttctaaatca tatgcaatca cccgaaatct
     7321 cgatcacaat cgatgatttg acagaaggga ttcgtagcgg attgattcaa ttagcaagca
     7381 accgcagtgc aatggcagag ctttctaaaa tattggagcc aaagataact gtagcaccaa
     7441 atgatacgat catgaaaatc caatcggact cctatgagtt accttataat ctatatgata
     7501 tcatcggaga taaagcagct ttactgaagt cggatggttt ggatcaagaa gcaatcaatc
     7561 atttcatttc tacagatatc aacatccatc tgaagtcttt ctataaagac catggttttt
     7621 cttttaatgc agacagcaaa ttagcagaat ttgtagatcc gaaaatcata gaagtgacca
     7681 atcagattta tgtaatggtc aaaagatctt taccatatga gtttcagcaa aactttattt
     7741 atgctatgag cttgcatatc agttcttttt tgaaaaaaat caacttagga gaagaacggc
     7801 atacgaatga taacatccgt gagatggctt ttgattttcc agatgaatat gctgttgcga
     7861 aagaagtgcg tagttatatc gaaacgtatt ttcaagtgga gattccagaa agtgaagact
     7921 attacttgac cgttctactc gtctcgttac gggcaaatca agcaagcggc agaatcggtg
     7981 tagtcgttgc agctcacgga aacagcacag caagcagtat ggtccaagtt gtccaacagt
     8041 tgttagatgc agagaacgta cgagcagtag acatgccttt ggatatggat ccgaaaacag
     8101 cattggaccg aattgaacgc aatgtacaag aagtagatga aggaagcggg gtcatactct
     8161 tagtagatat ggggtccctt gcgtcgttta attcgcaaat tcaaagagac actggaattc
     8221 cagtacgtac tgttgatatg gtaacaactt ctctagtttt ggaaactgta cgaaaggtct
     8281 cagtattagg gacagatcta gatatgttgc atgaatcatt gaaaaatttt cgaggttacg
     8341 cagaaatatc ttctgtagaa tctgttcagg aagcaaaacc tattcataag aaaaaagctg
     8401 ttttggctat ctgcgcatct ggcgaaggta cagcacaaag aatcaaagaa ttgatcgaac
     8461 gagcggtaaa caagcgtcaa gaaacagaat tagaagttct agctttgtcg attgtggaag
     8521 tgaaagaaga actgccagtt atccaaaaag aatatcagat catagctact actgggatta
     8581 ctgatttgaa tatccaagcc ccatttattc ctttagaacg ttttattgat caaaatatcg
     8641 aagtcatttt ggatcagttg ctgatggaat cagaattgga ggaaactgaa ttcatctccc
     8701 tagatgaaga gtcagctaaa aatacgtgtg ttgagtttat ctctgataac ttcatcttta
     8761 ttaatggtag taagttaatc gatccaatgt ggcaattttc tacacaaatc tctcagacga
     8821 cgggaatcgg cgatgaagaa tatggattca aaatcaacct agtgatgcac acagcaggaa
     8881 tgatcgaacg tattattcgg aatgaaccat tgactgtgga agaaaacgaa ttgacaaata
     8941 caacgaatga tcctctttat agccaattgg cagcctcagt tgttctgttg gaagatcaga
     9001 taaaagtaaa agtgccgata gaagaaatgt attatctgct acgtctagtc cataatcagt
     9061 tagataaaaa agaatacact gtgccatgaa tacacaaaaa cgatacactt ttatagtgta
     9121 tcgtttttgt gtattggtta agaatccttt aaattcaatt agtgtatcgt aaaaaagttg
     9181 gcacgctttt tgcttatagt agtgtgtgct aggaggtggt tgttcacaca gaaatgatag
     9241 gggtttctgt gaagtagtga ggaagaagga gttttgcaga tggatattcg attggcacgt
     9301 atagatgatc gtttaatcca cgggcaagtc gcaacagttt ggtcgaagca aacaggtgtt
     9361 gaacgtatca ttgtgatcag cgatgtagtc gctcaagata aattgagaaa gtttctacta
     9421 aaagaagctg caccgccggg gatcacagca aatgtaataa ccgtgcagaa aatgattcaa
     9481 atctatcata gtggattatt atcacgagca aaagtgatgc ttttattcac caatccccaa
     9541 gatgttgaag cggttgtccg ctgcggtgta cagttgaagt cgttgaatat cggcggtatg
     9601 agttttactg aaggcaaatc aatgattacc aattttgttt cagtaaatca acaagatatt
     9661 gattcctttc gttacttgaa cagtcaagaa gtggagttag agattcgaaa agtacctgct
     9721 gacaggaaaa tccttttgat ggaactacta gcgaaaatca aaaaggtgta gcaagaatgg
     9781 ttgttggaaa aggtcaaata attctgttgt aatggttagc aattattaat atttttgaat
     9841 ttgttacaac aaaagtaaat aggaggtata aatatggtag ggattatcct agcaagtcat
     9901 ggacaatttg ctgaaggaat cttacaatcc ggttcaatga tctttggcga acaagaaaac
     9961 gtcaaagccg tgattttgaa accaagtgaa ggtccagatg acttgagagc caaattggaa
    10021 gaagcggttg cttcttttga taatcaagac gaagtgttat tcttagttga tttatggggc
    10081 ggaacaccgt tcaatcaatc aaacacatta ttcgaagagc acaaagataa atgggcaatc
    10141 gtcagcggtt tgaatttacc aatgttgatt gaagcctacg catcacgctt ttcaatggaa
    10201 tctgctcatg aaattgcagc acacatcatt gaaacagcaa aagacggtgt aaaagtaaaa
    10261 ccggaagaat tagaaccggc agaagcaccg aaagcagcag ttgaagacgc acaacctaaa
    10321 ggagcacttc cagaaggaac tgtggtcgga gacggaaaaa tcaaatatgt attagcacgt
    10381 gttgactcac gtctgttaca tggacaagtt gcgacagctt ggacaaaagc agtgcaacca
    10441 aaccgtatta tcgttgtttc agatgcagta tcaaaggatg atctgcgtaa gagactaatc
    10501 gaacaagctg ctcctccagg tgtaaaagca aatgttattc ctattagcaa aatgatcgaa
    10561 gtagcaaaag accctcgttt tggaaatacc aaagcattgc ttttatttga aaatcctgaa
    10621 gacgtattga cagctgttga aggtggcgta gacatcaaag aattaaatgt tgggtcaatg
    10681 gcgcattcgg tcggtaaagt cgttgtaagt aaagtattgt ctatgggaca agaagacgtt
    10741 gaagcgtttg agaagcttga acaaaaaggt gtgaaattcg atgtacgtaa agtacctaat
    10801 gattcacgcg ataacatgga tgatattttg aaaaaagcaa aagctgaatt agcaaaagcg
    10861 taataaataa tattaaaatt tataggaggc ttatcatgtc tattatttca ataattttag
    10921 tcgtattagt tgcctttcta gctggtatgg aaggtattct tgatgaattt caatttcatc
    10981 aaccactagt ggcatgtaca ttgatcggtt tagttaccgg taacctcgaa gctggtatcg
    11041 tacttggcgg tacgcttcag atgatcgctt taggttgggc aaatatcggt gccgctgtag
    11101 caccagatgc tgcattagca tcagtagcat cagcaattat tttagtttta ggtggacaag
    11161 gtgttaaagg tgttccttct gcgatcgcga ttgccgttcc tttggctgtt gcagggttgt
    11221 tcttaacaat gatcgtacgt acaatcgctg ttccaatcgt tcatttgatg gatgcggctg
    11281 ctgaagaagg aaacatccgt aaggttgaaa tgtggcacat cattgctgta tgtttacaag
    11341 gggttcgtat cgcggtccca gcagcagcgt tgctatttat cccagcacaa actgtgcaat
    11401 cattccttga atcaatgcca gcatggttaa cagatggtat ggctatcggt gggggaatgg
    11461 tcgtagctgt aggttacgct ttggtaatca acatgatggc tacaaaagaa gtatggccat
    11521 tcttcgttat tggtttcgtt gtagctgcaa tttctcaatt aacattgatt gctctaggtg
    11581 ctttaggtgt tgcattagct cttatttact tgaacctttc taaaatgggt ggttcatcaa
    11641 ataatggagg cggaagcaac tctggtgacc ctttaggcga cattttgaat gactattaat
    11701 cttgaaggag gaaaataaaa tggcagaaga aaaaatcaaa ttatcaaaaa aagatcgttt
    11761 agctgttgcg tggcgctcta ccttcattca aggttcatgg aactacgaac gtatgcaaaa
    11821 cggtggctgg gcattttcta tgattccagc aatcaaaaaa ttatacaaaa caaaagaaga
    11881 acgttctgca gcattaaaac gtcacttgga attctttaat acacacccat acattgcttc
    11941 tccaatttta ggggttacat tggctctgga agaagaacgt gcgaatggcg cgcctgttga
    12001 tgatgtagcg attcaagggg ttaaagtagg tatgatgggt cctttagctg gtgttggtga
    12061 cccagtgttc tggtttacag tacgtccaat gctaggtgca ctaggtgctt cattagctat
    12121 gggaggaaat atccttggac caatcatctt cttcttaggt tggaacatta ttcgttgggc
    12181 attcatgtgg tatacacaag aatttggcta caaagctggt tctaaaatta ctgatgacct
    12241 ttctggcgga ttgttacaag acgtaacaaa aggtgcttca attttaggga tgttcgtatt
    12301 agctgccctt gtacaaagat gggtatcaat caaattctta ccagttgtat caactgtaaa
    12361 attagataaa ggtgcttaca tcgaatggga taaattgcct tcaggtggtg aaggaatcaa
    12421 gagtgcattt gaacaagtta ataatggctt agccctttct cctgaaaagg taacaacatt
    12481 acaagacaac ttagaccaat tgattcctgg tttagctgca ttaggattaa cattgttatg
    12541 tatgtggttg cttaagaaaa aggtttctcc aatcgttatc atccttggtt tattcgtagt
    12601 cggtgttgtt ggacacgtca tcggactttt ataatccata aaaatatagc tcgggcgttg
    12661 tcccgagctt tttttatata gtatactgtt cataaacaaa tatctggagg gagaaaatgg
    12721 ttcaatcaat caatacaaaa gtagacttgg taattgatgc tacttcgttt acgggtctga
    12781 cagattacgg gcaaatcatg ataggtgata atggttttga gttttaccat gcgcgagata
    12841 gacgcaaata tatccaaatt ccatgggaag aagtggatta tgtactagca tcagtcatgc
    12901 taaagggaaa atggattcct cgatatgcca ttcaaacgaa aaaaaatggt acatacacat
    12961 tttcttccaa agaacctaaa aaagtgcttc gtgcgatcag agaatatgta gatgaagata
    13021 gaatggtaca atcattgagt ttctttgatg tagtgaaacg tggtattcgt agtatgttta
    13081 aacgcaagta aaaaataaag aaaacttcta agaggaggtt ttctttattt ttttactttg
    13141 cagaagaaaa tatgaaatag caaaaccaat tatttgtccc ctttaaatcc catatgctaa
    13201 aattgattag caaagaagga ggttatatta tgagttggtt atggacattg attgttggag
    13261 ccgtgatcgg agcaattgca ggtgcgatca ccagtaaagg aaattcgatg ggctgtgtaa
    13321 cgaatatcat tgctggacta gtcggttcaa gtattggaca agcgattttc ggcgaatggg
    13381 gaccaaaagc tgcaggtatg gcacttattc catctatttt aggcgcagtg attttggtag
    13441 cagttgtctc atttttcttt ggaaaacgat cataagtttt tacaaaatag taaactaaaa
    13501 ggagttgcag cgagactgtg actccttttt ggctttattt atttaaaata aaaagaagtt
    13561 ttccggaaat ttttatttaa ctgtcgtgta cgaagaaaag tttgctataa tggaaaaaag
    13621 cgtgcaaaga aagagggagt ttcattatga tgctacgtgt agaaaaattg cgcaaaaaaa
    13681 tgcaagaaga aaatttggat tcatttttgg ttaccagtcc gtacaatctt cgttacttaa
    13741 cgaactttac aggtacgaca ggattagctg ttattacttt ggaaaaagca ttttttatta
    13801 ctgattttcg ttataccgag caggcggcag cgcaggctca agggtttgaa attattaaaa
    13861 atgtcggacc tatttttgat gaggtagcaa acttagtacg caaagaagaa ctgcactcat
    13921 tagcttttga ggaaacaaca gtctcatttt tagaatattc ggttttagaa gaaatcatcg
    13981 atgcggaatt agtacctgtt tcacagatga tcgaagaact cagagaagtg aaagatgaag
    14041 aagaaatcgc tattatcgaa aaagcatgtc atattgcgga tatggcatat gatcacatcc
    14101 tgaaaatgat ccaaccgggc atgactgaga ttgaagtcgc aaatcaacta gacttttata
    14161 tgcgttctct tggcgcttca ggtgtttctt ttgatacgat cgttgctagc ggattgcgtt
    14221 cggctatgcc gcatggtgta gcaagcaaaa aaatcatcga acaaggtgac ttgatcacat
    14281 tggactttgg ttgctattac gaagggtacg tttccgatat gacacgtaca ttcgctattg
    14341 gtgatcctgg ggaaaaatta aaagaaattt atcaaatagt cttagatgct caattagcag
    14401 ttattgatgc tgcaaaacca ggaatgactg gtgtccaatt agatgcagtt gctagagatt
    14461 atatttcaaa acatggttac ggggaagcat ttggacattc tacaggacat gggatcggtt
    14521 tagagatcca cgaaggacca aacgtttctt ctcgtgcaga aaagcaattt gtaccaggaa
    14581 atgtgatcac tgatgaacca gggatctact taccaggaat cggtggagta cgtattgagg
    14641 atgacttgtt gatcacagaa gatggaaatc gtgtgttgac tcgttcacca aaagaattga
    14701 tcatcttata gtttcatcaa tagaagaagt aagcgtaaaa atcagtgttc acagactttg
    14761 ttatagaaaa tcactcgatt taatgataca ctatagaaga acttttaagc tgtaagagat
    14821 aataaagttg ggtgttttct ccaattcata gcagtttgca gctgatcggc ggcagttttc
    14881 ttaccacgtg atcactatgc cgcatcatag agaattcgga tgtatctcgt gcttaggcaa
    14941 agggaggaat aaataaatgg cagatgaaaa aaatttgatt ttaaacgcaa accaagattt
    15001 aggagagatc gtgatcgctc ctgaagtcat cgaagtaatt atcgggattg cagcttctaa
    15061 agtagaaggc gtatacggaa tgcgcggaac ttttgccagc aatgtcactg aattattagg
    15121 acgtgcagct cacggaaaag gtgtttactt agcaaacgag gaagatggct tgaaagtaga
    15181 tctttactgt tatctagaat atggtgtttc agttcctaaa gtagcaatgg aaatgcagga
    15241 acgggtcagc cagcaagtcc ttttcatgac ggatatcgat ttatctgaag tcaatatcca
    15301 tgtagtagct gttgttcctg aaaaattaga acaaccaaca atcgaagaac tttttgaaga
    15361 cgaagaggaa gagaatgaat aaagaattat caagacacga gattcgggaa atggcattgc
    15421 aggctttatt tccattagac ttcaatgcag acctaacaaa agaagatgcg attttcaacg
    15481 caatcgaatt agaccaccgc gacatgatca acgaagatga atcagagttt gttcctgttt
    15541 atcttgatac gctagttggc ggtgtctgtg caaaaaaaga tgaattggat aaagtgattg
    15601 aaaaacattt aaaaaacaat tggcacatca atcggatctc aaaaatggat ttggtgatct
    15661 tgcgtatcgc gatttttgaa atgacttatg tgacagatgt accagcggct gttgctttaa
    15721 atgaagcaat cgagttggca aaaacattta gtgatgaccg ttcacgtaaa ttcgtcaacg
    15781 gtgttttatc caatgtattg aaagaattag aagctggggc gtaagtccta gcttttttct
    15841 atgaatcatc ataaacctgt tataaatcgg gaaaattcta tggtagaatg aaaacacaaa
    15901 aaatatctgg gagtggttag atggcagaat tgattaacgg aaaagaatta gctgaaaaaa
    15961 tgcaggcaga gattgctgaa aaaatccaaa aattgaaaga taatggtatc catcccggat
    16021 tagtcgtttt attggtaggg gaaaatccag ccagccaaat ctatgtaaga aataaagaac
    16081 gtgtagcaaa agaaatcgga atccattcat tggtagagcg ttatcctgag acgatttctg
    16141 aagacgaact acttgcagaa attgaaaaat acaatcaaga tcctagtttt catggtatat
    16201 tggtccaatt accattgcca aagcatatag atgaagaaaa gatcctgtta gctatccgcc
    16261 ctgaaaaaga cgtagacggt tttcatccga tgaatttagg cagactcctt gcgggtaaac
    16321 cggacaagat tccttgtact ccatacggca tcatgaaaat gtttgaagct tattctatcg
    16381 atccggcggg taaacgagca gtagttatcg gacgaagcaa tatcgtcggt aaaccgatgg
    16441 ctcagctact cttgatggca gatgcaactg ttactgttgc tcattctaaa accgaaaatc
    16501 tggctgaact ggcacaagaa gcagatattt tagtcgtagc aataggacgt ggacattttg
    16561 tcacaaaaga attcgtaaaa ccaggtgctg tagtgataga tgtagggatg aatcgtgatg
    16621 ctaacggtaa gctgatcggt gatgtgaaat ttgatgaagt ggagcctatt gcaggctgga
    16681 tcactcctgt gccaaagggt gtagggccaa tgactatcac gatgctgatg tatcaaaccg
    16741 taaaatctgc cgaagctttt ggagcaggtg aatagatggc acaagaatat ttaactgtca
    16801 ctacattaac gaagtatttg aaaagaaaat ttgatgccga tccttattta gaacgcgtat
    16861 atttaacagg cgaaatatcg aatttccgtt tacgtgccaa tgcccatcaa tatttcagct
    16921 taaaagatga ccatgcaaaa atatctgcta ttatgttcaa aggagcattc caaaaattac
    16981 gattccagcc aaaagaagga atgaaagtcc ttgtcgttgg gcgtatttca ttatatgaag
    17041 cgggaggttc ttatcaaatc tatgtagagc acatggagcc tgatggaata ggtgctttat
    17101 atcaagaatt ggaagaacga aaagaaaagc tgatgaagga aggccttttt caagggccta
    17161 aaaaaatgct gccacgtttc ccaaagcgaa tcgcggtcct taccagtcca agcggagcgg
    17221 ttatccgcga tatcatcacg acggtgaaaa gaagatatcc aattgtccaa ttggttttgt
    17281 tcccaacagt tgtacaagga aaccaagcag cggacaatgt tgtcaggaat atccagagag
    17341 tagaagagat gggcaatttc gatacaatga tcatcggtcg tgggggtggt tcgattgagg
    17401 atttatggcc gtttaatgaa gagcgggttg ctcgggcgat ttatcaagct aatacgccgg
    17461 tgatttcatc tgtaggacat gaaacagata ccacgatcgc tgatttagta gcagatgttc
    17521 gggctgccac tccaacagca gctgcagaat tagctgttcc tgttttgtca gaagagttga
    17581 tgcgcattca agaacggcaa gctagattgc agcaggcgtt tgtacgccag atccagcgca
    17641 aacaagaacg gttcgagcgt gctcgacgtt cctatgtatt tcggcagcct gaaagattgt
    17701 atgaaggtca gaccatcaaa ttagaccagt tgaaacagcg attatatcaa gctacacagc
    17761 aaatttatca tgcgaaagaa aaacaaacga tcgcattaag ccaccaattg gaacaagtgg
    17821 ctccgcttta tcgagttaaa gcagctcgcc aagaaactgc ttatctagaa aaacgcttag
    17881 aagagaaaat ccaacaatat atgaaacaac agcagcagcg ttttgctcaa gctgttcaat
    17941 ctcttgattt gttgagtcca ttgaaaatca tgggaagagg ctacagctat acgacgttaa
    18001 atgatcaagt agtaaaaagt gtcaccgaga ttcatcaagg ggatttgctg acgatccatt
    18061 atcaagatgg aatcgttaaa ggacaagtac aagagataga caaaggagaa taaaaatatg
    18121 gaaaatccaa cttttgaaga gtcactgcaa gaattagaaa aaattgtcat gcagttagaa
    18181 caaggagacg ttccattaga atcagctcta gattcgttta aacgaggaat ggaattaagc
    18241 aaacattgcc aagatacatt agctaaagca gaagagacct tgacgaaaat gatgacagaa
    18301 tcaaatgaag aagttgattt tgacggaaac gaggaaagct gagatgagat taactgactt
    18361 ttcagctatt catctacctg ctgtagaaaa agaaatcttg tcttttttag atgagcatac
    18421 aacggataga ggactctatg atgcgatgag ttattcagtg aaagcaggcg ggaaaagaat
    18481 acgtccctta ttacttttaa cggccgttgc ttcctttgat gagcctattg atgttcccgt
    18541 ctatcaagta gcagctgctt tagaaatggt gcatacgtat tccttgatcc acgatgattt
    18601 gcctgcgatg gacaatgatg atcttcgtag aggaaaacct accaatcata aagtattcgg
    18661 cgaagcatta gctatattgg ccggagatgg tttgcttaca ggagcattcc agctaatcag
    18721 tatggcacac ttaggtaatt ctcctaagct tctgctgctt cagcaattag ctgtttgtgc
    18781 gggatcacaa ggtatggttg ctgggcaagc tgcagatatc gagggcgaat cgaaaaagct
    18841 ttctttagaa gaattagcat ttattcatga aagaaagaca gggcatttga ttcgttatgc
    18901 cttgctggct ggtggcatat tagcgaagca acctgaagaa atcttattgc tacttcaacg
    18961 attagccgaa catttaggtt tagcttttca gataagagat gatctactcg atgtgattgg
    19021 tacaacgaaa acattaggga aaacagctgg taaagatgaa cgtatggaaa aaaatactta
    19081 cccgcgttta ttaggcctag aaaagacacg tgaggcgttg gagattgagc tgttatccgc
    19141 caacaagatc atagacaaat tagaagagga cgttttttct tttgatggcg ggctgatgcg
    19201 ccagatgatc aagcaatttg atgtggagaa atgagggaaa gaatggaaaa agaacgcgta
    19261 gatgtattag cggtcaaaca aggattattt gaaacaagag aacaagccaa acgttcagtg
    19321 atggctggtt tgatttacaa cgaaaaaaac gagcggtttg ataaaccggg agaaaaaatt
    19381 ccagtgagta gtgaattaag agtcaaaggt aaaaaacttc cttatgtttc tcgaggggga
    19441 ttaaaattag aaaaagcact caaagcattt gatctatctg ttgaaggaaa aacattgtta
    19501 gatattggtg catccactgg cggatttact gatgcagctt tgcaaaatgg cgcaaaaatg
    19561 agttatgctt tagacgttgg ctacaatcag ctagcttgga aaataagaca agatcctaga
    19621 gtcgtcgtaa tggaaagagt gaattttcgt tatgcaaaac ccgaagactt taccttagga
    19681 attccagaag ttgctgttat cgatgtttct tttatttcac taaaactgat gctgcctcca
    19741 ttacatgcta ttttaaaaga agagggagaa gtgattgctt taatcaaacc ccaattcgaa
    19801 gcaggcagag aagctgtagg gaaaaacgga attgttcgtg accctcagac ccatcaaaaa
    19861 gttttagaag acattttgtc atttgcggca caaggtggat atgatgtgct ggaattaagt
    19921 tattctccga tcacgggggg agaaggaaat atcgagttct tggctcactt gaggaaagta
    19981 cccgaaagcg gaacaatcaa ttcagctatt aatatggcag aagtggtttc aaatgcacac
    20041 gaacagtttg accataaata aattgtaact gtgaaaatca agtgagaagt atgcggattc
    20101 ttctttatta taccaaggaa ataagctatc attgattaag aagaatatgc taaggagcga
    20161 agacgtaatg agaaaaaaag atcggcatcg tttgattact cgattgttga gtgagcaaga
    20221 tatccgaaag caggaagaat ttgttgaaat cctgaaaagc aaaggaatat ctgttactca
    20281 agctacgatt tccagagata taaaggaact gaaactgatc aaggtaccgg cttcagatgg
    20341 cggctaccgt tatagcatac cagctgaaac aaacgaagat gtaaatacta aattggaaaa
    20401 actattaaaa gacgctttcg tggcagttga ccaaatggaa aaatttgtta ttttgaaaac
    20461 attgccggga aatgcttcgg cagcagcaaa tttgattgat aaacgttaca agaaagaatt
    20521 attttcgatt atcaatgatg acgataatgt tttgatgatt acacgaactg aagaccaggc
    20581 agttttattg aagaaagact tcctgcatta cctttaagtt gaggagcgaa tgtgatgcta
    20641 caagaaatca gtattactaa ctttgcgatt attccggaat tgcggctttc ttttcacgaa
    20701 ggaatgaccg cgctgaccgg agagactggt gcgggtaaat ccattattat cgatgcttta
    20761 ggtctgttag caggaggaag aggttcaagc gattatatac gtcaaggagc agaaaaatgt
    20821 gtgcttgaag ggttgtttga actaccaaaa caagaaggat tctctgaact gatggttgaa
    20881 ttagggatcg aaacagatga ggacaatctg attgttcgac gggatatgtc cttaactgga
    20941 aaaaatgttt gtcgagtgaa tggtcatatt atcacattag ctaatctgag aagaatcggc
    21001 agttatttag tagatatcca aggacaaaac gaacatcagg aactgctcca gcctgaatca
    21061 catcttgctt tattggatcg ttttggagat gtagctttcc aacagaagaa aaagagttat
    21121 cagcaagaat atatctctta tcgtgagctg gaaaaaaggg tacgaaaagt gcagcaaaat
    21181 gaaaaatcct atgttcagag aatagatatg ctccatttcc agcaagaaga aatagctgct
    21241 gctgatttgc aagtcggaga agaagagaaa ttaaaagaag aaagagaaaa acttagcaat
    21301 tatcaaaaaa ttgctgatgg attagctgca ggttacggag cactgacgaa taatgaacaa
    21361 aacagtgtag atggtgttgg cttggccgtt tcagaactgc aagggatcgc ccatctagat
    21421 gtggagtatg aagcaattta tgaaaatatc caaagtgctt actatttgct gcaagatgca
    21481 atcggcgata tgagtcgtca gattgattta ttggagttag atgagagtcg tttagaagaa
    21541 gttacgcaac gtttagaatt aatccgacag ttgaaacgta aatacggcga atccattgag
    21601 tcaatattgg cttactatga cgaaatcacc gaagagctag cttcttctga tttttcagaa
    21661 ggtcaattgg acaaaatgaa agaggaacta gagcaaaaag agcttctact tcagcaacaa
    21721 gccgcagatc ttcatgaagc gcgtaaaaaa atcgcaaaag aattggaaaa atccattctg
    21781 catgaattga aaagtctgta tatggaaaac actgagtttg aggtacgttt cttaaaagaa
    21841 gaaaatagac aattgaatca tgatggattt gaccaaatcg aattttacat tacgaccaat
    21901 ccaggagagc cactgaagcc tttggtgaaa gtcgcttctg gtggcgaact gtctcggatg
    21961 ttgcttgctt tgaaaactat tttttcttct gagcaaggtg tgacaagtat tatttttgat
    22021 gaagtggata caggtgtgag cggaagagtt gcacaggcca tcgcagataa aatttctcaa
    22081 atatcgaaat actctcaagt tttgtgcatc actcatctgc ctcaagttgc tgcagtcgcc
    22141 gactatcaat attatattgt gaaagcagtg attgatggac gtacgcaaac ttctgtttca
    22201 gagttgaaaa caaaagaaag agaagaagaa attgcacgta tgctggctgg aagtgagatt
    22261 acaaaactca cactcgaaca tgcaaaagaa ctattgaaac tcgcaaaaag gacctgagcc
    22321 acctcgcagt ggttcaggtc cttttcagca gctgatagct atattttatt tttacatgac
    22381 actcattaca gcagtcaata caacagatcc aatcgttaaa atcagcattg tccaaatgac
    22441 aaattttgtt actttagcaa aagtacttgt ttgttttttt tcacgcattg ttttcaacct
    22501 cgtcattaat tagttgattc aagtgtacaa tcttttttcc atttccacaa gagggctttt
    22561 ttataaaaac ttcccttttt ttaaataata ggtagttacg atacaaataa ataatgttaa
    22621 tagaactagc catataaagg catcatccct ttcagcaata ggtaacttaa tgttcattcc
    22681 atagatactg ccgacgattg tcggtatagt taaaataatc gttaaagaag ttaaaatttt
    22741 cataatatta ttcagattat tcgatacgat tgctgaaaaa gtctgattca tttgttctac
    22801 tagttttaat tgaatgcgag aagtagtcat tgcctgctct gtttccacca atacatcacg
    22861 taaatgattt ttcaaagcat gattttcttg gaaaagttta ttttgcgcta gttctttcaa
    22921 tgcatctaaa ttagcagaag tcgcttctct gaaataaacg aggctttttt ggatatccat
    22981 gatttgataa agttgtttgt tttcagtgga tacttgaagt ttggcttcca aagtatcgca
    23041 ttgagattga agcgtagcta aataactgtt gtaagagagt gtgacctgcc aaatgaattg
    23101 aaagaagata ttgagattag gcgaaatatc actttttgga aaggtctggc taaagacttt
    23161 ctttaaaaac agtggttcat gattcgtaat tgtgataagc ttctttttgg gcgtcaaaat
    23221 aatagagagc gggtatgtat ccacttgaaa ataaccgcta ggacttgttt tagcaaaagg
    23281 atattgtaac aataataacg ctggctttgt caactttgat tgattgaatt cttctgtacg
    23341 ggaattttct gcatcgtcca atatggaagt gatgtaatct gacggaattt caaattgatg
    23401 tgtcagctga tgaatctctt cttgattagg acgttctaca caataccaga tcgtttggtc
    23461 agtttccttt ccggtattac gcagtttctc attatctaat gtaaaatatt tgatcatgat
    23521 tttcctcctg ttgcctgttt cttttattat aacggatagg gaattggaat acatggaatg
    23581 tgtttgtaag atatagaata taactgcata aatttaaata aaatgaaaca gttgtgtaaa
    23641 gtatatacag tatacttgaa gcaacgataa aaaaggagtt tacgttcatg ggcacattca
    23701 caccacttat cttgttttgg tatatttacc cggtaatcgt tttgtttggt tgccaatttc
    23761 ttgtaaaatt attttcgtta aatcgtcgtt ttaaaataaa agcccctgat ttggcgattc
    23821 cttttttgtg gggcggtatg catgcattgt ctagaaatac agggacaatt tctatcttac
    23881 cgtttttctt tatttctgtt ttgctaatgg gaatttgtat tgctgttttc caagcatatt
    23941 attatgaaga aattatttat ccgcgttatt tcaagatgac ttggcgtttg gtttttctgt
    24001 tgacaatcgt cttgtacgct gtcttgatca ttgtgaacat actttcttac ctatgaaaaa
    24061 aatagaaaag ctgtaaaaag tcttttaaaa ggctttttgc agcttgattt ttaaaaaaat
    24121 gtttatgaat aaaaaaagtt gttcccggat ttcccagaaa aggtggtaga aagtggggga
    24181 ttgtggtaga ctaattttag ttagtggagg aatggggggc tttcaatgtt gatgggtgaa
    24241 ttccaacata atattgatgc aaaaggacgt ttaatcgtgc cctcaaaact tcgtgaagaa
    24301 cttggcgaaa agtttgttct gacacgcgga ttggatggct gtcttttcgg ctatccgatg
    24361 agcgaatggg aaaatctgga agctaaactg aacgaaatgc cgttggctaa aaaagacgca
    24421 cgtacttttg tgcgattctt ctattcggct gccactgaat gcgaacttga taaacaagga
    24481 cgaatcaata tacccagcac gttaagaaat tatgcagctt tgacaaaagg ttgcgtagtc
    24541 atcggtgtat ctaaccgaat tgaaatttgg gatgagacac gatggcaaga tttttctgca
    24601 gcagcagaag agaactttga cgaaattgct gaaagtatga ttgatttcgg actataagaa
    24661 taggaagagg ctgactaatg actgaaacat ttcaacacta tacagtcatg ttgaaagaaa
    24721 cggtagatgg attgaacatc aaaccggatg gcgtttatgt agactgtaca ctcggcggtg
    24781 ccggacatag tgaatattta ttaagccaac taagttctaa agggcatctg tatgcgtttg
    24841 accaagatca aaaagcaatc gatcacgcca agctgcgttt agcttcttat gtcgaacaag
    24901 gtatggtcac atttatcaaa gcgaacttca gagaattaga agaacgcttg agtgagcatg
    24961 tagataaggt agacggcatt ttgtatgatc taggggtttc ctctcctcag ctagatgaag
    25021 cagaacgtgg atttagttat catcaagatg caccgttgga tatgcgcatg gatcagtcag
    25081 ctcctttatc cgcttatcac gtaattaatg aatattccta ccatgaatta gtaaagattt
    25141 tcttccgcta tggcgaggag aaattttcca aacaaattgc tcgtgaaatc gaacgagtac
    25201 gaaaaacaaa accaatccaa acaactggtg agcttgtcga aatcattaaa caagtcatcc
    25261 cagctcccgc aagaagaaaa ggcgggcatc ctgcaaaaag aatttttcaa gctgtgcgga
    25321 ttgcagtaaa tgacgaactc ggtgccgaag aggcctcttt agaacaggca atccggttat
    25381 tgaaagtcaa tggaagaatc agcgtaatca cattccattc attggaagac agaattgtca
    25441 aaagtatgtt taaagaatat agtacggtac aagatctacc accgggactt cctgtcatac
    25501 ctgacgaatt ccaaccagaa ctgaaagtca ttactcgtaa accaatcctt ccttcagagg
    25561 aagagctaac agaaaacaat cgatcacgca gtgcaaagtt acgtattgca gaaaaaataa
    25621 gaatgaaaga ggagtagaat gatggcagaa ttaaagaaaa tgcaagatta cgaatatgat
    25681 ttacctgaat tggtggatca gccggaacaa caaatgcctg attcttcttc gaatcaagaa
    25741 atcattcttc cacaatcacc ggcaaaacga ttaaagcaga tttctgcttt ggaaaaaatt
    25801 gctgtggcaa gcgtggcgat tgctattgtt gctttatgtg ttttaacagt tatgctgcgc
    25861 acaaatatca gcggagctga aaaagaaatc acgtcaatac aaacagaaat caatgataaa
    25921 aagcaagaaa aaacgagttt agaacaagaa aaaaacgaac tttctcgtac tgaacggatt
    25981 aaacaaatcg cagaagaaaa aggattgtct attaatgacg acaatttaag gaaagtgaag
    26041 taaatgagtc ttaaaaataa aattcggcgg tttatggaaa agaaaaatct aaatccgatg
    26101 aacaatcgca aaaaagtagg tatcatttta tttgctacga gtattggatt gttcttttta
    26161 tttgccgtcc gtttctcgta tatcgttatt ggaggtcatg ttgctggtac ctcattagct
    26221 gaaaaaacaa aacagttgta ccaaggaagt gaagtagtaa aagcaaaaag aggaacgatt
    26281 tatgatcgta acggtgtggc gctcgcagaa gatgcctcct cttattcaat taaagcgata
    26341 ctctcaaaaa catatacctc tggggataaa aaactgtatg ttgaagaaaa gaattttgat
    26401 aaaatagcag aaatcctgca taagaatctt tctatagaca aaaaagatgc actgaatatt
    26461 ttagaggacg gcgcaaaaaa agagctgtat caagttgaat ttggtagcta tgggaaaaat
    26521 atctcccaag aaaccaagca aaacatcgaa gcggacatga aaaaagaagg tgtcgctggt
    26581 ctttattttg tcgaccatca ggctcggatg tatccaaatg gcgtcttttc ttctcatttt
    26641 attggatacg cagttccaga caaagatgag aatggcttag ttggtaaact aggtttggag
    26701 tctgcttaca atgatatctt gagcggtaaa gatggtaaga ttatttatca aaaagataat
    26761 tttcaaaatc ctctgccagg tacagtggca gaagaagaaa aagctgtcga cggacaagat
    26821 atctacacga ctttagacag cagactacaa agttatttag aaacactgat ggatcaagta
    26881 aatgaagaat atcagccaga agaattgaca gcagtcctga tgaaagcaaa aaccggagaa
    26941 atcttggcaa tgggacaacg acctactttc aatcctgaaa caatggaagg attgactgga
    27001 aaagatgcta tctggcgtaa tttcttggta caagatagtt atgaaccagg atcaaccatg
    27061 aaagttttca ctactgcagc tgctatcgaa gaaggagaat tcaacgagaa tgaaaccttc
    27121 caatctggaa aaatccaagt agaagatgct acgatcaatg accatgactt tggtgaaaaa
    27181 ggtgtgctga ccatgcgtca agctctttca tggtcaagta acgtcgggat ggtcatactg
    27241 gaacagcgat taggcggacg atggtataac tatttacaaa aactgggatt tggacaaagt
    27301 actcattctg gattagatga tgaagtaaac ggtgctttac ctacattaaa tattgtcgac
    27361 cgagcaatga gtgcttatgg acaagcggta ggtgtgacga atttccaaat gatgaaagct
    27421 ttcacttcta ttgcgaataa cggtacaatg attcaacctc gatatatcag caaagtggtg
    27481 gatccccaaa ctggagaaga gcgaacaact caaacagaag ttctagggca acctttttca
    27541 aaagaaacaa cagaaaaagt ccgtgaatat atgagagatg ttgtcgaaag cgaaaactac
    27601 ggtagtgctt atggtgtata tagtgtacct ggatacaatg tatcggcaaa aaacggaaca
    27661 gcccaaatcg cttctgatac agggggatat caaacaggag atacagccta cctgtattcg
    27721 attgtggaga tggttccttc tgaagatcca gactatgtct tgtatctaac aatgaaacac
    27781 cctaagacat atgatcgaat ggcattggct aaaatcgcaa atccattgat gaaacgggca
    27841 atggatttta aagaaactga agaagattca gatacagaaa caaaaacaga aaaagtatcc
    27901 gtagccgatt atcgtaatct tgaagcggac gttgcagcag ctgatgctca aaaaagtgga
    27961 ctgcaacctg tagtgatcgg taatggtaaa aaagtacaaa aacaatctac tgccaacggt
    28021 gatcagttga tctctggaga aaaactgatc ctatatactg gtggtgataa gctgatgcct
    28081 gacgtaacag gatggtcaaa agcagatatc atgaagctgg ggaaaatcct cggtattgaa
    28141 gttagttttg atggagatgg ttattgtgtc aaacaagaac ttgctccata tgaaaaaatc
    28201 actgaagaca aacttagttt tactttagaa gaataagaag agaaatagga gagaaaagag
    28261 catggaatgg acacaagcat tgattcccat tgtcagcagt tgtgcaatga caattgccgc
    28321 aatgccgcta tttattggtt attttcaaat gaaaaagcaa ggtcaggcga ttcgtgagga
    28381 gggaccaaag tggcacaata gcaaagccgg aactcctaca atgggcggct tagtgtttct
    28441 gatcggctct attttgacag gaatttgggt tggagcttgg caaaaacagc taactcctac
    28501 tcttttcatc ttgctttttg tattagcgtt atacggagta atcggctttc ttgatgattt
    28561 tataaaaata ttcaagaaaa gaaacatggg actgaactcg aagcagaaat tgattggaca
    28621 gattctaggg ggaatcatct tttatcttgt ttaccgttct gaaggttatc caggtgtatt
    28681 gaatttcttc ggactagatc tacctttagg aatcatttat ggtattttcg ctctcttctg
    28741 gcttgttgga ttttcaaatg cagtaaatct gacagatggc atcgatggat tagttgctgg
    28801 cttaggaagt atctcttttg cagcttacgg tctaattgct tggcatcagc atcagtatga
    28861 tgtattaatc atttgcttaa gtgttttagg aggtttactg ggtttcttcg tctacaacag
    28921 aaaacctgcc aagattttta tgggagatgt cggttcatta gcgttgggcg gacttttagc
    28981 ggcaatttct ataatgctaa accaagaatg gacattgctt ttgattggtt tgatctatgt
    29041 tatggaaaca gcaagtgtga tgcttcaagt tacttccttt aagcttacag gaaaacgaat
    29101 ttttaaaatg tctccaattc atcatcactt tgaaatgtgc ggatggtcgg agtggaagat
    29161 tgatacagtt ttttggttga tcagcattgt gacttcgttg atcacattat ggatcgtttg
    29221 gtaggagatg acgggaaatg aaaaaaatat caacatatga aaataagaaa gtgcttgtct
    29281 taggactcgc aaaaagcgga gtaagtgcgg ccaaattatt acatgaattg ggcgcacttg
    29341 tcaccgttaa tgatggaaaa ccttttgacg aaaatccgga agcacaagag ctgctttctt
    29401 tagggataaa agtaataaca ggtagtcatc caatcgaatt gttggatgaa gaattttcat
    29461 tgatggtcaa aaatccaggg attccctaca gtcatccttt agtagcaaaa gcccaagaaa
    29521 tgggtattcc ggtaattacg gaagttgagc tagcttacga agtagcggaa tgcccaatta
    29581 ttgggattac aggaacgaac ggaaaaacaa cgacaacaac gatgacagga ctactactga
    29641 atgcaggagc agatcaagga attgcacgtt tagcgggtaa tattgggtat ccagcaagtg
    29701 gtgtagcgca agaagcaaaa tcagaagata aaattgtgat ggaattgtct agcttccaac
    29761 tcatgggaat cacagatttc cgccctcata tagcggtgat taccaatatc tatgaagcac
    29821 atattgatta tcatggtaca agaaaagaat atgtcaaagc taaatggaac cttcaaaaaa
    29881 acatgacaga aaaagattat ctgatcctta actggaatca gtcagaacta caagaattag
    29941 cgcaaagaac aaaagcacgc gtactgcctt tctcgacaaa agaagtgttg gaagacggtg
    30001 tgtatgctga cgattactca atatactata aaaaagaaaa aataatggaa atatctgaac
    30061 ttggcgtacc aggtaagcat aatgtagaaa atgcactagc tgctatttct gtagcaaaat
    30121 tgtatggtat atcaaatgaa gctatacgag aaacgttgca ttttttccat ggcgtaccac
    30181 atcgtacgca atatgtagga gaaatccaag gcagaaaatt ttataatgat tcaaaagcaa
    30241 ccaatatttt agcgactaaa atggctctaa gtggatttga aacgtctaag gtcgtgttat
    30301 tggctggcgg attagacaga ggaaatacat ttgatgaatt aattccttca ttaaaaggaa
    30361 tcaaagcaat ggtcgtattt ggccaaacaa aagaaaagtt gatggatgct ggaaaaaaag
    30421 caggcatcga gacaatagta acagccgatt ctgtagaaca agcagttcct ttagcattag
    30481 aaaacagtac agatggtgat gtagttttat tatcaccagc gaatgccagc tgggatcaat
    30541 acccaaattt tgaaactaga ggaaacagat tcatggaagc tgtgaaccga ttaaaataga
    30601 aaaagtagaa gcgagcgata aaaaatgaaa attttggtta caggcggagg aacaggggga
    30661 catatttatc cagctcttgc ctttgtcaat tatgtaaaga ctcaagaacc gaatgctgaa
    30721 ttcatgtatg tgggagcaaa acgaggtcta gaaaacaaaa tcgtccctga tacaggaatg
    30781 cctttccata ccttggagat tcaaggattt aaacgaaaaa tttccatgca taacatcaag
    30841 acggtccgat tgtttcttaa gagtatccgt caagcaaaaa aaatcctcag tgattttcaa
    30901 ccagatatcg ttatcggtac tggaggatat gtttccggag cggtagtgta cgctgcagcc
    30961 aaaatgggta ttccaacagt tatccatgaa caaaacagtg taccaggtgt taccaataaa
    31021 tttttaagcc gatatgtgga tcggatcgct ctttcattta aagatgcggc ttcctttttt
    31081 cctgaaaaca aagcagttct tgtaggtaat cctcgagcac aagaagtagc agatactaaa
    31141 aaatcagata ttttgaaaac atttgatttg gatccagaaa agaaaacagt cttgattttt
    31201 ggcggtagtc aaggagcact taaaatcaat caagcagtat cagttttttt aagtgaatat
    31261 attccttctt cttaccaagt tttgtatgct tcaggagata gatattatga agagatccta
    31321 aataaaataa atcctgaaat acaaaatatt agtgtccagc cttatatcaa aaatatggca
    31381 gaggtcatgg cttccagtga tctgctggtg gggcgtgctg gtgcaacttc gatcgcagaa
    31441 ttcacagctc tggggttacc ggcagtgttg gttcccagcc catatgttac gaatgatcat
    31501 cagactaaaa atgcaatgag tcttgttcac gcaggagcag caaaaatgat tgcagataat
    31561 gaactaacag gtgaatcact aagtcagaca gtcaacgaaa tcatgggtga tgaagaattg
    31621 caaaagcaaa tgtgccgagc ttctaaagaa caaggaatcc ctgatgcttc aaaacgtctt
    31681 tatgacttag taaaacaaat aatcaaaaaa taaaggcggt ggtcttagtg agtaagaaaa
    31741 aagaaccaaa aaaattgtct catccgtctt ctgatgaaaa caatttgact ccttggcaaa
    31801 aggctaaccg aaaatatctg gcagaacaag agatgaaaaa ccaagagcca aaaaaagaag
    31861 acataacatt agatacaccc ctttcagatt ctgaagaaga aaaaacggtt caagaggaac
    31921 agcaagaaga aagccagtcc gagaaaaaaa tcgaattttt tgaagagatc aagcaaaaga
    31981 aaaacggtgg accatataac ggttcttttt tgaaccggct gcccaattta aaaagtcaac
    32041 ggcacaaagt actatttcgt cggttagctt tgatcatcac tgtattgggt ataccgctga
    32101 tttttttgat ctattatgtt tctccttata gcaaattaca agcagttgca gtatcaggca
    32161 ataagacagt caacagccaa gaaatcatct ctgatacaaa attgtcatta ggagaaaatg
    32221 tttggggaca gtattttcat cgatctactt atatcgatcg ccttaaaaaa gcacagccaa
    32281 gaatcgaaac agcaaatata catttcaaag ggatgaatga attcgatttg gatgtaacag
    32341 aatataaaga gattgctttg atcgcaaaaa acaatcaata ctacccagtg attgaaaatg
    32401 gaacagtatt agatgaaaaa gtagcaaatc ctacaaaaaa tctccctatc ttggaagagt
    32461 tcaaagactc tgcaaaaatc aaagaattaa caaagcaata caatcagtta tcatcagaat
    32521 tacaaaaagc aatttctgaa atcaagtaca cgccaagagc aagcaataaa aatctgatcc
    32581 aattgaatat gaatgacggc aatcaagtta ttgtaaatat caataatctt gcgaaccaaa
    32641 tgaaatatta ttcgcaagta gccaaagata tggatgaaaa aggtgtgatc gacatggaag
    32701 ttggtatctt ctcgcatcct tacggtagca ctcaagaaac ggaagaatcg ggccaatcgg
    32761 aagaaacaac tgaaagtgaa gaaaatgcgg atcaaacaaa cgaatcaagt gaagaggcag
    32821 aaacgcaaaa agcacaaaca cctggatcgt ctagcacaaa cggatcatct gattcaaatg
    32881 attcaagtga aacaagtcaa atagaagata atccaagtac aactccgtca agtttaaatg
    32941 aaaattgata aaacaacttt aaaaagtaag aatgtacttt ctaaaaacca gtaatttatg
    33001 gtagaattgg aagcagtgaa tatatctatt aataagtata ttttaaaacg gacgctagtt
    33061 gttcgcttgt atatggaata gtaagtagga gggaatccca ttcatggcaa aaacaggaat
    33121 gtatgtaggc cttgatattg gaacgacatc tgttaaagtt gtcgtggctg aatatatcga
    33181 cagccaaatg aatattattg gagtaggaaa cgcaaaatca gaagggatta accgaggcat
    33241 tatcgttgat atcgataaaa cggtccaagc gatacaacga gcagtacgac aagcagaaga
    33301 aaaagcaggt atccagatca aaggcgtaag tgtcggtttg ccagcaaaca tgcttgaagt
    33361 agaaaattgc cagggaatga ttgcagttaa cggagattca aaggaaatca ccgatgaaga
    33421 tgtcagaaat gtggcttcag ctgcccttgt tcgttcaata cctcctgaac gtcaaatcgt
    33481 gtcaatattg ccacaagatt ttactgtcga tgggttcgag ggaatcaaag atcctcgtgg
    33541 aatgatcggc gtaagattag aaatgtacgg tctgttgttt acaggaccta aaacgatcat
    33601 ccataacata cgtaaatgcg tggaaaatgc cggattaatc gtcaacgaaa tggtgattac
    33661 tcctttagcg ctgactgaat caattctttc tgacggagaa aaagattttg gtacgatcgt
    33721 cattgatatg ggtggcggcc aaacaacaac ggctgttatg catgataagc aattgaagtt
    33781 taccaatctt gaccaagaag gtggcgaatt tgtcactaaa gatatctcta ttgtattaaa
    33841 tacgtcattt aacaatgcag aagcgttgaa aataaactac ggggatgcat acccagaacg
    33901 gacatcacca gatgaagaat ttccagtaga tgtgattggt cagtcagaac cagtgaaagt
    33961 cgatgaacgc tacttgtcag aaattatttc agctcgaatg gaacagattt tcaacaaagc
    34021 aaaagatgca ttggaccaaa tcgaagcatt agagttgcca ggtggcgtcg tattgacagg
    34081 cggagctgct agtcttcctg gtgttgtgga cttggcacaa gaaatcttcg gcgtaaatgt
    34141 aaaattgtat gttccaaatc acatggggct gagaaacccg gtatttacaa atgtaataag
    34201 tatcgtggat tattcagcaa acttgagtga agtgtatcaa ttggcaaaaa ttgctgttac
    34261 gggtgaaacc tcagcagctc gtcaaatggt agttgaacaa gaaacaacaa acacatatga
    34321 gagctatgaa gcacctgaag aaacaatcta cgatgagccg gaaccaaaag agtccggaga
    34381 aaatgtaaaa aataaaatca agggtttctt tacaaatatt tttgattaat tcatcctggg
    34441 aggaataaga tatggaattc tcaattgata ataatatcaa tgacggcgca gtaatcaaag
    34501 tcatcggagt cggtggtggc ggtggaaatg ctgtaaaccg aatgattgaa gaaaacgtta
    34561 aaggtgttga attcattaca gccaacacag acgtgcaagc attaaaaaat tcaaaagctg
    34621 aaacagttat ccaattaggc cccaaatata ctcgtggttt aggtgctggt tctcagccag
    34681 aagttggaca aaaagcggca gaagaaagtg aacaatcctt acgcgaagct ttagacggtg
    34741 cagatatgat tttcatcact gcaggaatgg gtggaggaac cggtacaggt gctgcaccaa
    34801 tcgttgcagg tatcgcgaaa gaattgggcg cattgactgt tggtgtcgtg acacgtccat
    34861 tcacttttga aggaccgaaa cgtggccgtt ttgctgcaga aggtatcgct cgtttgaaag
    34921 aaaatgtgga tacattatta attatctcaa ataaccgttt attagaagtt gtagataaaa
    34981 agacaccgat gcttgaagct ttccgtgaag cagataatgt tttacgacaa ggtgtacaag
    35041 gtatctctga tttgatcaca gcgccaggtt acgttaacct tgactttgct gacgtgaaaa
    35101 cagtgatgga aaaccaaggt acagcattga tggggatcgg tgtggctagt ggagaagatc
    35161 gcgtagtaga agcaacgaaa aaagcaatct cctctccatt gcttgaaaca tctattgatg
    35221 gagctgaaca agtacttctt aacatcacag gcggtttgga tatgacattg ttcgaagcac
    35281 aagatgcttc agatatcgta gcaaatgcag caactggcga tgtaaatatc attctaggta
    35341 catctatcaa tgaagaaatg ggcgatgaaa tccgtgtgac ggtcatcgca acaggtatcg
    35401 atgaatcaaa aaaggaacgc aagtctagcc gaccagctag acaagcacaa atgcaatcac
    35461 ctgctcaaaa gactgtatta gatatggatc aagctaaacc aacttcttca gaagaagaaa
    35521 acagttttgg cgattgggat atcagacgtg aacaaaatgt tcgtcctaga gttgatgatt
    35581 ccaattttga caatatcgaa aaaaaagaat ttgatacatt caaccgcgaa gaaacaaaat
    35641 ctcatggcga tgatgaatta aatacgccac cgtttttccg tcgtaaaaaa taaaggagag
    35701 gaagagcaca tgattgctga caacttgcag aaaatcaagc aagaaattca ggattcgtgt
    35761 gctcttgttc atcgtgatcc gaaagaagtt acattagtag ctgtaacaaa atctgttgaa
    35821 tccgctcaag ctgaagaatt ggttagagct ggcgttatga attgtgcaga aaaccgagta
    35881 gataaattat tagaaaagaa acaagattta tttgattttc ctgaaataaa gtggcattta
    35941 attggtaatt tacagcgcag aaaggtaaaa ttgattgtaa atgaaattga ttactttcat
    36001 gcgttggaca gtttaagatt agcagaagaa atccagaagc ggttagagaa agaattatcc
    36061 tgttttattg aagtaaatgt ttctggtgag gaatcaaaac atggaatttc accagatgag
    36121 cttttaccgt ttgtggaatc tttagctgcc tgcacaaaaa tcaaggttgt tgggttgatg
    36181 actatggcgc ctaaagaagc ttctgatgag caaatccata aagtgtttgg tacgctgaaa
    36241 gaattgcgtg atcttgttca agcaaaaggt tatgcccatg caccatgtag tgaattaagt
    36301 atggggatga gtcaagactt tcaaattgcg attgaagaag gtgcaacatt tatccgtgta
    36361 ggaacagcgc tattcaaaga cgaataaagg aggaacaaaa atgccgcttt taaacagagg
    36421 agcaattgct aattttttcg gattagctga tgaggaagag tacgaatacc aagattatcc
    36481 tgaaacaaca aaaaaggctg tacagccaac taaaaaaaat acaacacaga atcaatcaac
    36541 tcattctgta tctgctgcga atactcatga aaaacaaaaa acggcgactt cacgtcctgt
    36601 aacgcatgaa agtattcagc cggctgaaaa gaaaaaagta attgaactac atcaatcacc
    36661 tacaaaaact gtagcgaaag aaagaacagc ttcaagaacc tctacacaac ctaaagcaaa
    36721 tcttgctgga aaaatcactg taatggaacc aagagcttat tctgaagcta tggcgattgc
    36781 taaacgaatc attgctggag aagcaacctt agtgaatttt catttagtag aagaaaaaca
    36841 agcgagaaga atcgttgatt ttctaacagg aacagtttat gcactagatg gagatatcaa
    36901 acgtgttgga gatgagattt ttctttgtac accgactggg atggagattg acagttcaac
    36961 cgctcaatct tttgcagatg cgaatttgtt tgatctgtaa gaggaggaac tacgatttat
    37021 actattgcac tattacttca tttacttcaa cgggcagcct atttctatag cattatcttg
    37081 atggtatatg cattgctctc ttggtttcca ggcgggtatc aatcagggct aggacgatta
    37141 ttagctaaga tctgcgaacc gtatatcagt ttgttcgatc gtctgccttt gaggattgga
    37201 cccttggatt tcacaatttt aggggcaatc ctgatcttga acttagccgt acaaggttta
    37261 gcacagatcg ttatatggat aactgtccat ttagtttatt gattgaagca acaaaatctt
    37321 tattctcaga atagatagga ggggaaaatt ttggatgcaa atgtatacca gcactttcgg
    37381 gcagatgaac gtacatttat cgattcggta ggcgattgga ttgaacaggt tcaaggacaa
    37441 tatgcaccct atctgaccga atttcttgac ccaagacaat cctatatttt agaaacactg
    37501 attcgacaaa gcagtgattt gcgtttcatg ttttatggcg gatatgaaca ggcagaaaga
    37561 aaaagatgtt tgattttccc cgattattac gaacctgtag aggaagattt tgaaacagca
    37621 ttgtatgaaa tccattatcc tagcaaattt gccagcctaa gtcatgggaa aatccttgga
    37681 actttagtgg gaacggggat caaacgctct tactttggcg atatcatctc ggacggtgat
    37741 cgctggcaag tatttattgc aaatgaggtg gatcattttg ttgctgcaca agtaacaaag
    37801 attggaaagg ttgctgtacg cttggaacca atcaaataca cagaaatgat tatgccgaaa
    37861 gatagctgga cacaagaaag aggaacagtt agttccatgc gactagacag tgtgatctct
    37921 gagatttata atatttccag acagcggtcc aagcagctga tcgaagctgg taaagtaaaa
    37981 gtgaactgga cagaaaatac aagacctgat tttgtacttg aactattaga tattatttca
    38041 gtcagaggtt atggcagagt ccagatccaa gaacttgagg ggaagacgaa aaaagaaaag
    38101 tacagggtac tgttcggtgt attacgtaaa taaaataaaa aggtggttta ctcatggctt
    38161 taactccatt agatatccaa aataagacat tcccgacaaa aatgcgggga tataatcaag
    38221 acgaagtaga tgatttctta gatttagttg ttcgtgacta tgaggaattg actcaaagaa
    38281 atcgagaatt agaaaaagca gtcaaacatt ctgaagaaaa acttgagtac ttcaatgaat
    38341 taaaagatgc tttgaaccaa tcaatcatcg tagctcaaga tacggctgat aaagtaaaaa
    38401 caagtgcaag caaggaatcc gaagtgatcg ttacttctgc acaaaacaaa gcagatgagt
    38461 tagttgcgaa cgctgaaaaa cgtgcacatc aattaacaac tgatgcagaa gaaaaagcaa
    38521 gaaaaatctt gacagatgca acagaaaaag caagacagct tgcgacagaa actgaagatc
    38581 ttaagaaaaa aacacgagta ttccatcaac gtatcagctt gatgcttgag tctcaattag
    38641 aacaagtgaa aagtccggaa tgggatgaaa ttttacaacc attctccagc tacgtgactg
    38701 acagccatga agtgatcaaa gaagttttag caaaacagct tgacaatgaa aatgatactg
    38761 atgtaaactc agacacagga gtcatagaag cacctatgac ggcatttgat acacaggcta
    38821 ttgatatctc gatcgtgcct ggtgaaaatg aaaacgagta gaacagaaaa gcactttgtt
    38881 attcttaaag cgagccgatg agagtggaag ttcggtaaga cactgcatgg tgaaagatcc
    38941 tctacttgag acctgctttt ccgaaattag agtaagaagc agcggctgac ctcgttattg
    39001 gtcacagaga gtttgcacta gatagatgca actaaatttg ggtggtacca cggcatgttc
    39061 gtcccttgcg gctgaacatg cttttttgtg tccggaaaac aaataaagga gtgtatggtt
    39121 gaatgaaaat gaaagaaacc ttgcaattag gaaaaacaaa attccctatg cgtggtaatt
    39181 tgccaaaccg agaaggcgag tggcaaaaag aatgggaaga aaatcgaatc taccaaaaaa
    39241 gacaagagtt gaacgaagga aaacctagct ttgttcttca tgatggacct ccatatgcca
    39301 atggtaatat ccatttagga cattcattga ataaaatcag taaagacatt atcattcgtt
    39361 caaaatcaat gtctggctat cgtgcaccat atgttccagg atgggataca catggattgc
    39421 cgattgaaca agtattggct aataaaggcg tgaaacgtaa agaaatgtcc atggcagaat
    39481 acttggaaaa atgtcgtgaa tatgcacttt cccaagtaga taagcaacga gcagatttca
    39541 aacgcctagg agttgcagga gattgggaac atccttatgt tacattagat ccttcttatg
    39601 aagctgcaca agtacgcgta tttggtaaaa tggctgaaaa aggctatatt tacaaaggat
    39661 tgaaaccaat ttactggtct ccgtcaagtg agtcttcttt agcggaagct gaaattgaat
    39721 ataaagatgt aaaatctcca tctatttttg tagctttcaa tgtcaaagac ggcaaagata
    39781 ttcttgatga agatacagct tttgtcattt ggacgacaac accatggaca ttgccagcaa
    39841 accaaggtat tgctgtaaat ccaaaattta cgtatgtatt ggtggaagcc gatggccgta
    39901 agtttgtcgt agcgaaagat ttgctagaaa cagtccaatc agaaatcggt tgggaaaatg
    39961 taacagttct aaaagaattt gctggacaag aaatggaata tatgactgca agtcatccat
    40021 tctatgaacg tgaatcactt gtgattttag gcgatcatgt tactttagac gctggtactg
    40081 gattagtcca tactgctcca ggacatgggg aagatgacta tatcgctggt agcaaatata
    40141 aactatctgt agtgtcacct gtagactcaa aaggattgtt tacagaagaa gcacctggat
    40201 ttgaaggtat cttttatgac aaagcaaatc ctatgatcac tgatctatta aaagaaaaag
    40261 gagctttgct gaagctagat ttctttactc atagttatcc tcacgattgg cggacgaaaa
    40321 agcctgtgat ctatcgggca actccacaat ggtttgcatc tatcgataaa ttccgtcaga
    40381 atattttgga tgaagtagaa aaagtagagt gggttatccc atggggtaaa acacgtttgt
    40441 ataatatgat ccgtgaccgc ggcgattggg taatttcccg tcagcgtgca tggggagtac
    40501 cacttccgat tttctatgct gaaaatggag aagcaatcat cacaccagaa acaatcgaac
    40561 atgtggcaaa attatttgct gaacatggat caattatctg gtttgaaaaa gaagcgaaag
    40621 aacttcttcc tgaaggattt acccacccag gttctcctaa tggcgaattt acaaaagaaa
    40681 aagatatcat ggatgtctgg tttgattctg gttcttctca tgaagctgtt ttacgtcagc
    40741 gaccagaatt atcattccca gcagatatgt atttagaagg atcagaccaa tatcgtggct
    40801 ggttcaactc aagtatcacg actagtgtgg ctatcaatgg tgtagcacca tacaaatcag
    40861 ttatttctca aggatttgct ttagatggtg aaggccgtaa gatgagtaaa tctttaggaa
    40921 acatcattgc accagaaaaa gtgatcaaac aatttggtgc agatatcctt cgcttatggg
    40981 tatctagtgt agattatgaa gcagatgttc gtgtttctat ggatatctta agtcaagtgg
    41041 cagaagtata tcgtaaaatc agaaatacga tgcgtttctt attagcgaat acagaagatt
    41101 tcgatcctaa aaaagatact gtttcgtaca atgatttacg ttctgtagac aaatatatga
    41161 ccgttcgatt gaaccaagta atcaaagaaa tccgagaaga agggtacgac aaatataact
    41221 tcatgcatat ttaccgtaca gttatgaact tcttgacggt tgatctatcg tcattctacc
    41281 tggattttgc taaagatgta gtttatattg aagcagaaga tgattatcaa cgtcgttgca
    41341 tgcaaactgt tttctatcaa acagctgtag ctttgacaaa attattgact cctatcattc
    41401 cgcatacagc agaagaaatc tggtcattcc tgaaagaaga agaggagtac gttcagttaa
    41461 gcgagtttcc tggttatgaa gagtttgcaa accaagaaga attgatggat acctggactg
    41521 ctttcatgga cttccgtgat aatgtcttaa aagcattgga agaagcaaga aacagcaaat
    41581 tgattggtaa atctttggaa gcaaaactaa ctgtttatcc aaaacaacaa gttcgtgaaa
    41641 tgttaaaagc tttggatgca gatattgcac aattattgat cgtttcacca gactactttg
    41701 aagttatggc agcagatgaa caagtgccag ataacgctat ggtatttgat gatgttgcta
    41761 ttacagtgga aaaagcagac ggggaaactt gcgatcgctg tcgccaagtt cgtaaagatg
    41821 taggagtaga tgaaaaactt cctcatctat gcggtcgttg tgcaaaaatc gttgaagact
    41881 tctatcctga agctgtagct gaaggattcg aagaaaagta aaagggaaaa gggggtgtga
    41941 catgttcttt gtcacacccc cttttgttat caatcgttta attttccacg ttcacgatac
    42001 cagttatctg gttgccagat ccattcgaat ccgtcttttt caagtagttc gaaagctgct
    42061 ttaggtccca ttgatccagc tttgtagttg ggaaaatccg gttgtgtttg gtcccaagct
    42121 tttcgaatga catccacgat atgccaagat tgagcgactt cttcccaatg agagaagttc
    42181 gttccatcac cattcaacgc atctaatagt aatttttcgt aagcttctgg gctattttca
    42241 accatttctg cactatttct aaattccaat ttgattggtt ccgtttcaaa tccttgccct
    42301 acttctttcc cattcaagga taaagaaaat ccttcagttg gctgaatata gatcgttaga
    42361 acattaggtg gaagtgtcgt atcgtcacat ggttcatcga cagatgtttt gaatacatta
    42421 acaggaactt gtttgaatac gatgttgatc cgagtccctt tttcagtcaa acgtttgcct
    42481 gtacgtacat agaaaggaac acctgaccaa cggaagttat cgatagtgaa tttgcctgcg
    42541 acgaatgttt cagtctctga ttgagcatcc actttgtctt cttcgcgata gcccttgaat
    42601 tgtacaccat ccaattcacc ttgagcatat tgcccgcgaa cgaagttttc taacacttct
    42661 tcttcgttat aaatgcggat agctttcaat gctttgatct tttctgtacg gatttctttt
    42721 tcggaaaatg ctactggcgg ttccattgca agtaatgcta caacctgtaa aatatgattt
    42781 tgcaccatgt ctttaagtgc gccactatga tcgtagtagc ccccgcgatc ttctacaccg
    42841 aggttttcgc caaaagtaat ttggatattg tcgatatagc gattgttcca ttgagcctca
    42901 aaaatattat tggcaaaacg gatagctgag atgttttgga tcatttcttt tcctaaataa
    42961 tgatcaatcc tgaaaatatc ttgttctggg aatacttctc tgatttgctg gttcaattca
    43021 aaagcagact ggtaatcaga accgaacggt ttttctatga tcaaacgatt gaagccattt
    43081 tttgtcataa tattttgtga tttcaaatga gaaacgatcg ttccaaaaaa ttgaggtgcc
    43141 atagccaaat aatagacacg gttaccgttt aattgatagt gtccatctaa tcggtcagac
    43201 aattctttta atgtctgata atgcgttgaa tcatttacat catgggattg atagtaaaaa
    43261 tggcttgcaa aggtagttgc ttcttcatct gaaggattca atgcacggat tgtttctttg
    43321 acaacttcac ggtagtgctc atcgctccag ggacggcgag cggttccgat tacagcaaaa
    43381 tgctcgccta aatttccttt tctgtaaagg cggaacaaag acgggtacaa tttacgctgg
    43441 gctaggtcac ctgtaccgcc gaatatcgta aacaatacat ttttttcatt agacatgtgg
    43501 atacactcct ttatatttta tcgacaaaaa tcgtgctggc cgccttaaag ctgacgggga
    43561 ttttctttct atcattttgg atagtgatcg gtccttcata agctgcgatg tcaacgatct
    43621 catattcttc gttgatattc aggtcaatag tgactaagta atccaacaat tctttttcat
    43681 caagcactcg tgcgatacgc acttttgtac cgacaggata ggactccaat gtttgtcggc
    43741 gttcttcatg gacaacttcc tcttttttag gaatcgatcc tccatgagga caatgttcag
    43801 gatgattcaa atagttttct aaatgatctg caagatgttc cgaagtcaca tgttccaata
    43861 cttcagcatc atcatggact tcattccagc tatagtctaa atgttcaact aaaaaaactt
    43921 cccataagcg atgtttgcga atcagactgc tagctttttg cagtccgctt gcagtcaatt
    43981 gtacaccttg ataaggggaa tgttctacaa gctcttcttt cacaagttta gaaatcattt
    44041 cactgacaga tgcagctgag acatctagac tagatacgat ttgtttattg ttgactttcg
    44101 ttgtatctcc acctagttct aaaatgattt tcaaataatc ttctcgattt ggtgtcatat
    44161 tcgatcatcc tttcatggta aaaaaccatc attagtaagt gtaacaaaaa aaacgttaat
    44221 tgactatttt tctaaataaa aaacaaagga tttttagacg tttttacaac aaaaaacgaa
    44281 agatgataga gaagactcat tctttcgttt ataaaggata tatatataca ttgggaggga
    44341 tctatttttc agataacaac ttatatgaag tgtctgcaga ttcgtcataa agagtttgtt
    44401 cgggataaga aaagataaga taaaaaccat actgatcacg tacgactaaa cgatattcat
    44461 ctatttctac cacagatgtg ccaagagact gaccgttgat caaaaacgtg taatcagatg
    44521 taatctgtaa ttgttgtttt tgctggctat cactaaagaa ccatgtacct tgatactttg
    44581 agagtccttc tgacttttcc gcttgttttt tctttttcaa gtaaataaaa cttgagagaa
    44641 cggtaatgag tgaaccaaga aaatagatca ttttttttat caccaaatca ctcctttatg
    44701 agaaaataat cgcatagaca tattctttct tctttatttc attgtaccga ataaaagcta
    44761 aaatttctct aagactttcg actggttttt ttactgtttt tctacaaatt atgataggat
    44821 acatagtagg aagaaaggtg acataatttg aagtatcagc aagtggaaat agcttttttt
    44881 attgaccgcc cgaatcggtt tatagcacat tgtatgaaaa aagacggaga aataattatt
    44941 gcgcatgtca aaaatacagg tagaggaaaa gaggtcctga taccaggcgc tgaagtggct
    45001 attgtatttg ctcctggacc taaacggaaa accgcctatg atctgattgc tgtaaaaaag
    45061 aagggtgact ggtttaatat cgatagtcaa ttgcctaatc ggctggcaat tgatggtatt
    45121 ttagacggca cgatcctttt accaggatta actggtccat ttacggtata caagcgtgaa
    45181 gtaacttatc ttcactccaa atttgatatt tatttagaga cggtggtcgg acaaaaagca
    45241 tttgttgaag tcaaaggtat gacacttgaa aataaagcga ttggtgcttt tcctgatgcg
    45301 cccacactca gaggattgaa acatatagga gaattaacgt atgccgcaca agatggttat
    45361 gccgcttatg tcttgtttat tgctcagttc gaacatcttc atctggcaac gatccatgaa
    45421 gaaatgcaac cagcactagc tgatatggtt cgacatgcgc agcaaagcgg agttcaaatc
    45481 ctcgcctata attgtcaagt atcggcagat gaggtaactg tcaagcaggc cattccattt
    45541 gatgtagata tcccttttga agatcctata caatagaaag aaggtaaaat aatgatcaac
    45601 ttaggtatta tcggaacaaa ttggattact catcaatttg tccaagcagc tttaagcaca
    45661 aaaaaatatg aattgacagc ggtatattct agacatttgg aaaaagcaca ggaatttggt
    45721 tctcattatg aaggggatat cgaattcgca acagatttga agactttttt caatattact
    45781 catatggaca ccatttatat tgcttctccg aatagtcttc actttagtca agctaagcaa
    45841 gcaattttgg caggaaaaaa tgtgatagtt gaaaagccag ctttttcgac gccaaaagaa
    45901 atggatgaaa tcatccattt ggcaaatgaa cacaatgtgt atttttttga agcagcaaga
    45961 aatatccatg aatcaggttt taaaaagatt gccgattact taccattaaa agacgagata
    46021 atcggtgcaa atttcagcta tatgaaatat tcttcgcgct atgaccaagt attagatgga
    46081 gaagaaccga atatcttctc gactcatttc tcaggtggtg cattgatgga tttaggtgtt
    46141 taccttgtat atgcagctgt tgcttggttt ggaatgccaa aagaagtcca ttatttccca
    46201 agaaagattt ctactggtgt agatggtctt ggatggggga tcttacgtta tgagaatttt
    46261 gatgtatcga tccagccagg taaaatcggt gattcttatt taccgtcaga gatttatttt
    46321 gactcaggaa cgctagtgat gaatggagtc aatgcaattg aacaagcaca atttcatgat
    46381 cgcgcgcata aagaaatcat tgatttggcg gtaacagctg cagaaaatcc aatggcagat
    46441 gaagcagagg attttgccaa tgtcatggct catccgaaag atccagcttg gggattgttg
    46501 tatgaagagt ggatcgagct agctagaaat gtgaaccaag tgatttatga tttaagaaaa
    46561 aatggcggca tcaaattcga tgcagataat gaggaagatt tttaatgaac gaatttgtac
    46621 aagtatttcc cgaaaattgg cagaaaaaat atgaggataa agggtttatc tcaccttctc
    46681 ttattcaacg agcagttttt cagccactaa gtaataaaca aagtatagta gcggtttctc
    46741 ctacaggttc aggtaaaaca ctggcttatc tttggcctct actactaaac gtggaaccag
    46801 gtgaagcaag tgcgttggtc atttttgctt cttctcaaga actagctatt caagtagcag
    46861 atgttgcacg tgaatgggga aaagacaaag agttgaaagt ccaatctttg gtgggcggag
    46921 ccaacgtcaa acgtcagatt gagggtttga aggaaaaacc agaaatcttg attggtacac
    46981 caggaagaat attggaattg atgaaagcaa aaaaaataaa agcacatcaa gtgaaaacaa
    47041 tggtatttga tgaagcagac cagctatttg acgctgggaa cagccaaatc attgatcaaa
    47101 ttcttcatca agctccaaca gagtatcagc tagctttttt ttcagcgaca gcagatcgtt
    47161 cccttgaaag cattgaaaaa atcactggga aaacgtttga gacaattgac gtgacagcag
    47221 aggacgattc tcgaaaagga ctgagacatt actttttacg tgtaccgata cgaaagaaag
    47281 atgactactt gagacgtctc acgcatatag aagattttca tggtctgatt ttctttaatc
    47341 aactgactga attaggcgta atggaagaaa aattgctgta tagaggtgta ccggtaggaa
    47401 gtctggcctc tgatcagaac aagctactcc gaaaagcagc gctagaacaa ttcaaaaaga
    47461 aaatggtcag agcacttttg accacagatg tggcggctag aggtttagat attacaggtc
    47521 ttccttatgt agtgaatgca gaagttccgt taagtgaaga agcttatttg catcgttcgg
    47581 gaagaactgg tcgaatgggt aatgaaggaa tagtaattac actcgtacaa gataataatc
    47641 taagagattt aaaacggctt ttacgtccat caaatataac acttgaagag atttttttat
    47701 acggaggtaa gctgcaaact gagcagccaa ccaaagaaga tgcagctcct gcagtaaata
    47761 aaaagtatgc gaaaactttt tctggaaagg agcagaaccc agaatataac aaagaaaaaa
    47821 taagaaaaaa agttaaaaat aaatcaaaaa aagagagaaa taaaggagca aggcgcaaat
    47881 aagcgccttt ttatgctatg attatgaaaa cgggaacaaa attggttgcc agaggagggg
    47941 ttatgtgaaa gaacgtcaag aagagatctt gcagctattg acagaaaacc aacaaggatt
    48001 gacagcaagt gatgtagctg agcgcttaac tatcgatcga agcaatgcaa gccgatattt
    48061 aagtgaacta tacaaggccc atcatattgt aaaaaccgct ggccgaccag tcgtttactc
    48121 tttgccaacg gaaaagtcaa aatctgatga agtgcatgtc gactcttcta cgcaagtgac
    48181 ttttgaaaca ctcgttggag aaaatgattc tttaaaagtc agcatacagc aggcaaaagc
    48241 tgcgattctt tatccaccgc gggggttgca tacgattatt tttggagaaa ctggtacagg
    48301 gaagtcgatg tttgctgaat gtatgtatca ttttgcaatc gattcagaga tgctttctgc
    48361 agatgcgcca tttgtttctt tcaattgtgc ggattatgct caaaatccgc aactgctctt
    48421 tggacacatc tttgggatca aaaaaggggc ttacacagga gcagctcaag atagtcccgg
    48481 tctaatagcc aaagcagacg gcggtatatt gtttttagat gagatccacc gtttaccgcc
    48541 ggaagggcag gaaatgctgt ttactttcat tgataaaggt atttaccgtc cgcttggaga
    48601 aagtgcacaa gtacatgaag catctgttca gatcattggg gcgacgactg aatcttcaga
    48661 aagtttctta tctacgttca atcgacggat tcctatggca atcaccttgc cgaatcttgc
    48721 ttcacgttca ctggatgagc gttatgaaat catctctctt tttatcaaac aagaagcgaa
    48781 tcgtttgaat caacgtatcg atatcgaaaa agaagcgatt cttgccttca tgctttatga
    48841 tgcggaagga aatatcggtc aagtgaaacg agacttgaaa ctagtctgtg caaaatcttt
    48901 tttacattac cgtactcatc atgagaaaaa gctaatcatt cgaaaagaag aactttccct
    48961 tcaagtacaa aaaggattac tgaaaatcaa ggaaataccg gaacgtctcg accgattcat
    49021 ggacagcaaa agtcagtatt tgacatttga accaggattt gctgatgttg tctggtcaca
    49081 agatccagaa cgaaacatgc aggtctataa cgatattgag gaaaaagtca cttctctttc
    49141 cgaaaccggt gtggagaaca ttgatctaga gaccctcatt tcaaaagatg tggatgctta
    49201 tttccagaca tatgtcaaag aattgactaa aagttcgatt cccaaagatc ttttgccaga
    49261 tgaaatctgg cggttaacca atcaattgta tgatgtggct gaaaaacggt tagaccgtat
    49321 ttataatgaa aaagctcgtt ttgcttttgc tttgcatctg caaagtacat tggatcgtgt
    49381 gagagaaggt catatgatcg tccacccaga tttgaataac gtacggaaaa attttaagag
    49441 ggaattccag gtagcaatcg atctttcaac gatcattgaa gaagaagaac aagttgagat
    49501 tccttttgat gaaatcggct ttattagtat gtttctttct atcaatctag gagaaagtga
    49561 gcctgttaaa gaaaacaaag tagatgttct catattgatg cacggcagac agactgcttc
    49621 aagcatgttg gagatggcac aagaactatt aggaacaaaa gtagggaaag cttttaatat
    49681 gccactggtt atggaagttc aaacaatgta tgaagaagtg ttacaatatg tcaaaaacta
    49741 tcaagagcat ttgacgaatg gcctactctt gttaacagat atgggatcat taaacacctt
    49801 cagtgatttg atttatgaag agactggcat tcggacgaaa gctatcacga tgacaagtac
    49861 catgattgtg atagaagcga tacggatggc aagtgttgga agaagcttag aagatattta
    49921 tcaaaatatc cagctttcct ttgaatcgat cgtacgtgat cagttcaaag cggaaaagtc
    49981 tgaaacttta cagcgtaaaa aagccgtaat cgtgacatgc tttactggtg aaggagtagc
    50041 tgccaaatta tatcagcgta ttatgccagt gattaatcaa aataaggtag aactgatcca
    50101 gatgcagttt ttagaacgtg aatcattcaa aaaacacatc gatgccttat tggaagaata
    50161 tgagatcaaa gcaattgcgg gaacagttga tgttgattat caaaatatcc catttttttc
    50221 agcctatgac gtttttgatg atgaaaaact aaatatttta aagcgtatag ctgatgatga
    50281 gatcccgaca gctaaaattg tcgattcact tgctggatca ttaagcgatg tcttttctat
    50341 tgatgaactg atccgttcat tgcaaaagac agttcagcag atccagaccg atttgtcgat
    50401 tatcgtggaa cctggagtag atactggaat cgttatccat ttagccttca tgttagacgg
    50461 attgatcaaa caagaaagcc atcggcaatt tgagaaactg tctattttct caaaaattta
    50521 tcgtttggaa atggatatcg ttcgggcaaa cttgatggca ctagaacgaa aatatcaggt
    50581 aaaaattcca gaagatgata tcgcatttct tacacaaatg tttcttgaaa ataaaataaa
    50641 aaaatgaaca cactaatcat actgtttaca cactaaacat ctgtgtgcgc actataaaaa
    50701 agagctaaaa agcatgaaaa cgctatttca gctctttttt ttattggcat gcttcttgcg
    50761 ttatataaaa gtgtaaaaaa ataaaaggag tgttaaaaat ggcaaaaaaa acaatcatgt
    50821 tagtttgttc agcagggatg agtacgagtt tattggtcac aaaaatgcaa aaagcagcag
    50881 aagaaaaagg gatggaagcc gatatttttg cggtctctgc ctcagatgca gataataatt
    50941 tagaatctaa gaatgtcgat gtattattat taggtcctca agtacgtttc atgaaagctc
    51001 aatttgaaca aaaacttgcg cctaaaggta ttccattaga tgtcatcaat atgcaagact
    51061 atggcatgat gaacggagaa aaagttcttg cacaagcaga aaatctaatg aaataaatca
    51121 agtatgcagt tgggggtata acaacgtgga agatcaacaa aatctagaag ccatcatggg
    51181 gttaatcatg tttggcggta atgcaaagag cgatgcaatg gaagcgattg cggctgctaa
    51241 aatcggtgat tttgagttag cggataggaa aatcgctgat gcagaagaat cacttataca
    51301 agcgcatcat gcacaaacag acatgttgac acaagaagca caaggaaatc atatgcaagt
    51361 gacattattg actgttcaca gtcaagatca cttaatgaca tctattgcgt ttactgattt
    51421 agcaaaagaa atcatcgaac tttatcgtcg cgtatacagc gaataaatac aacttctttt
    51481 ttgaacggaa gaagaaaaat ttttgaacgg attttttatt ttgttataag gagaactctc
    51541 tttatataaa aaacttatag gaggaagtaa aaaatgaacg gactatcaag ttggatggag
    51601 aaatacatcc tgccggttgc cgcaaaaatc ggagcgcaaa aacatttagt tgctttgcgt
    51661 gatgctttta tcggcacact gcccgcaaca atggcgggat cagtcgctgt aatgatcaat
    51721 gcaattattc gtgatttacc gcaacagttt aacagtacct atgctgacag tttagcagca
    51781 gacaaaggaa tctttgccgt attgaatgtc atcatcggaa tcaatggttt tgtctggaac
    51841 ggtacactag caatcgccgg attgatcttt gccttttctt gggggtataa tcttgcccga
    51901 gcttatggag taaatgatct agctgggggg atcgttggtt tagctacctt gattcaaggt
    51961 atttcctttg gtccagcatt atcagcagca ttagatgtga atgttccttc aaatgtcgca
    52021 gatgcaatca atagttctga gattggtgca acgattgcag atggcacact gtcagttgga
    52081 ttatggggtt ggctaaaact agatcatttg aatggaaacg cttattttac agttatgatc
    52141 atgggagcta tttcagtcat tattttttgt aaattgatgc ttgccaacat tacgatcaaa
    52201 atgcctgatt cagtaccacc agcagtatct aaagcatttg cagccatttt accagcgacg
    52261 atcgctttat acgtcattgc tattatcaat tatatcgtag gtcgtgtagc tgatgggcaa
    52321 ctgatcattg atttggtgca aaaatatatt gcagagccat tcctaggatt atctcaaggg
    52381 ttgggagcag tattgatcgt cacagtattt gttcaagtat tctggttctt tggtattcat
    52441 ggaccaaatg tattggcacc agtgctagag ggaatctggg gacaagcaca actaatcaat
    52501 attgatattt tccaaaaagg ttataacgga aaaactggta caccagccgt gttagatgca
    52561 atcgaagatg gaaaagctta tatgtgggtc cgcggatcgt tcgatgcttt tgcatggttt
    52621 ggcggatcag gcggaacgat cgtcttactg atagctatct tgattttctc taaacgtgca
    52681 gattatctaa cagtagctaa attatccatt gggcctggta tcttcaatat caacgaacca
    52741 gtgatgtttg gtctgccaat cgtattgaat gcaatcatgt ttatcccatt cttgattgct
    52801 ccagtagtag caacagcaat tggatggaca gctacttatc ttggcctagt cgctccggta
    52861 tcgcagcaag tcacatgggt agtaccgccg ttcttattgt cattcttagc aacaggagcg
    52921 gattggcggg caccgatcgt aacattagta tgtatggtcg tgactttctt gatttgggca
    52981 ccatttgtca tctctgcaaa caaaatggac cctgccgcag gcgaacaaca ataagatgaa
    53041 cgtgaggagc gggacgcttt aagtttcgtt cctcataatg aattttcgag gaggaactga
    53101 aatgattaca attgccagtg caatgcaaca agatgaaatc aaagaattgt tagaaggata
    53161 caatgaagaa ggaatgactt ttacatttaa agaaaaacaa ggaatcaagc tgttctttga
    53221 aacaagtgca gaagatccgg aagttgcggc taaaaaagca aaggaactaa tcaaagcaca
    53281 gccttgggga acagtccttt attttcaatc aacagctgtt tagaaaatgg tttaaaaaga
    53341 taggatggtt gagatgataa aaaacggttt atttttatgt agtattggtt ctttgatcgt
    53401 gttgtatagt ttgaatccac attcgcaaag gtatgatttg tatagtatgg tcacaggtct
    53461 ttttctaaca ggattaggaa tctatttctt catgaagggg aaaaaaagag aagaagcaaa
    53521 ggagaaaaat aactaatgga aatcacgaaa aaattaaata ttccagcatc gtttttttac
    53581 gataaagtta tggactctgt ccttttcgat gtacgtaaag cgaccggcaa atcagtaact
    53641 agaaaacagc tgaaaaattt ggagtacgtc aaacaattct cgcaaaataa ccgggctcgc
    53701 ataaaaatcg aagaagtcat tgaaaaccag tcttataagt ttcgtacatc gacaacgaag
    53761 aatgaattcg tcgttcatta tcaaatcaaa ccattggacg attcgacctg tgaggttcat
    53821 tacacagaaa aaatggagtc ttatggcatg cttcaaaagt tgaatgatat gcttttagga
    53881 acaatcttga tgtattttaa acggagacgt ttcaaaaaaa tgctagatat gatcgaagca
    53941 acgtattaaa tcaatgaaac atggtactat ttagttattg aaaaatgaag taagctagaa
    54001 ggaatttttc ataggaagat agatctttcc tgcgaatcct cactagcttt tttgtaggaa
    54061 ggggcagcaa aatgacaaac aaagtaagag agctaggtgt atcttcgatt gaggaaatgt
    54121 ttgatttagc cacttatgca tttaatgggg cagatacacc tgaaaggcga gaacgttttc
    54181 gtacacttgc ggagaattct tggaattacg gattttttga tgaggaggat cggctaacaa
    54241 gtcaagtcat ggcgacccct tttccagtga atttttacgg acaagagtac ctaatggccg
    54301 gcattggcta tgttgcttct tatccagaag caaggggaca aggcgggatc aatcagatca
    54361 tggaaaagat ccttgaagat tgccgtgatc gtaaagtaag cttatcttat ctggcgccat
    54421 tctcctatcc cttctatcgg cgctatggct atgaacaatc atttgataaa atcagttatc
    54481 aactaaacag tcgtgatgtg ccgtttatca aaaaaaaatc tggaaaaata aagcgtatgg
    54541 attttgaaga tgcaaaagct gcaatcagaa gaatttatga acagatgccg gaaaaccaac
    54601 gagctggtct gaagcgcgaa gactggtggt atacgtataa attcaagcaa aaaggcaatt
    54661 accagtttgc tttgcatttt gatgataatg aggaagcaac gggatatatc gtttatcagc
    54721 ttgcaacgtc ttcctttatt attgcggagt ggggattttt ggatcatgat gctttttgca
    54781 gccttgttcg cttcgtaagt tctcacaatg gtgcttttga aacatttgaa tattcatgcg
    54841 gctacggcgg taatgatcaa aattatctat tagaaaatcc atttgcttct gtaatgatca
    54901 caccttatat gatggcgagg atcgtagata tttctttgtt tttggaaaat tatccattta
    54961 aaaagagaca agctgcattt gccctacaaa tagaagaaga tcattatgcg aaatggaatg
    55021 aaggaatcgt ggaagtaaca atcgaaaatg gaaaaaatca tctctcaaaa gtagaacaaa
    55081 caaaactccc aatcgttcga ggaagtatcc aacatatcac gcagctatta ttagggtatc
    55141 gtaaagtgga agagttggta ttccaagaaa gaatacaggt gacaaaagaa tatgaagaaa
    55201 cgttaagctc aatcttgcca cagcaacagc caattcttaa tgactatttt tgataaaaaa
    55261 cgggtgatga caatattttt gtcaccaccc ttttgttttt catttggcat ttgaaggaaa
    55321 atgttaaagt aaatcctctt ttttcgtaaa tttgaatttt ttgttttctt ttgttttagg
    55381 gttttctttt ttgtggtctt ggattgcttc tttcgtaatc ttttcccgat tttttttaga
    55441 tgaatctaag cgttctgcgt atactttttc ttttggctta tcattcattg gaaaggacct
    55501 ccctacatta tttcattcta cctctagttt atcatctgtt aaggactagt ggaaacgttt
    55561 tgcctaactt ataagctgat ttttttagca gataagcttg caagtaaaaa acaaacaagc
    55621 tacaattgat ttattgaaat tgttcctgta gctcagtagg atagagcaat cgcctcctaa
    55681 gcgataggtc gctggttcaa ttccagtcag gaacgtacaa ataacccctg tatcctttgc
    55741 ggtacagggg gttattttgt ataagtagac attgtagttt ctaaactgct atgtcctaaa
    55801 cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc atgcgtatgt
    55861 cttagtccat caataaacag tcgaagagaa aatagggaaa aattgattac taaagaaaaa
    55921 cttactaggt cgacggtgta gttgattttc atacttcaat agattcgaat taaacgattt
    55981 cgattggctt ataccagtcc tatccaacga aagatatatc gttcttaact ctagcggtat
    56041 aacttgaagc tttacgtaaa gccaatggga atgaactgga gatgactttt tattattttt
    56101 agtaatagct gatgggttaa tacttacaac tttccttttc aaagtaccat ataaaaaaac
    56161 ggtggggttg tttagcgaaa taaaggatct aagccaatag cttagatcct ttattctatg
    56221 ctttatatta ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa
    56281 aatcggtact gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac
    56341 catgaaatct acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga
    56401 catttctgta ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga
    56461 tgcgttttca tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg
    56521 gtcaggtaca agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt
    56581 tgctaagaca tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt
    56641 aaatttcata cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta
    56701 accatctact cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat
    56761 gaatttttct ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa
    56821 tccgataatg atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt
    56881 caattcttct gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc
    56941 ttcttcatag acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc
    57001 ttctggcagt tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt
    57061 ccaactgact ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc
    57121 tgattgataa gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga
    57181 aacaacgctg atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga
    57241 ttgtggtaca ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg
    57301 gtcgtcagtc cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat
    57361 caatcgagca aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc
    57421 tgtggctacg ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc
    57481 agataacata ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca
    57541 tttaattatg attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt
    57601 taagtttcat gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta
    57661 attttgcata tcaaaaaaac atgttacaat gaaattaatc aatggtggaa gcgttataaa
    57721 aaaattaagg agattaaaaa atatggcaag aaaaaagacg attactagag aacagatttt
    57781 ggcagctgct tatgaagtag ctgccaccga tggatttaca cgatttactg cgagaaacat
    57841 cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt gagtttaaaa atatggatga
    57901 cttgagacaa gctctttttg agcagatcta tgatcattta caatatgaag tgttcactgt
    57961 tgagcataca gggaatacga ttatcgatct tgcttttaat tatatccatt ttgcaagaag
    58021 agagaagaag ttgtatcgtt cactgtactt agaagaatat ggtggaggaa aagagatgca
    58081 gaatttctct tatacttact ttgtgaatac agtgaaacgc gatccagctt atgtgaatct
    58141 ttcagaagag cagatcgaat ctttgtataa tgggacatgg atcatagcta ctgggatcgc
    58201 agctttgatg tcttcaagca tcatccatcc aactgatgaa cagattgaaa agatgatcca
    58261 agacagtata gatgctatct tgaaattcaa agaaccaatc gaaatcgatt gaatatactc
    58321 aaaagttcca caaatcagta ggtaacaaga gatgtgtcaa aataaggaaa agttcgtttt
    58381 tcttacagcg tatgcatttt taactcccga agaactcttc catgaatgca atgaataaaa
    58441 gagccataca gattgaataa atctggatgg ctcttttatt gcaagtatat taaatttttg
    58501 cgactagttc tttagcgaaa gcttctaaat tttcgatatc ttcttcttca gcattcagat
    58561 ccactttaac attttcggct ccttttgatg cgcctgtttt tgcaaaagca gcatcgaaat
    58621 catccaccga tttgcagaat tcatcgtaaa aagtatcacc agatccgcag acaccgtaaa
    58681 tttttcccaa tagatccaat tcttgcaagt cttcataaaa gtctacgatc tcatcaggca
    58741 gatcgccatc atcgtatgta taagtagcta ctacgcaaat atctgcctcc tcgaattcat
    58801 ctgcatctac ttgggtacat tcgttgattt cgacttcgat atccagattt tcaaatgctt
    58861 ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat gctagcatat acgatttttg
    58921 ctaaagccat gatgtgcctc ctcgtttata attatttaac aattatgaat tataccaaaa
    58981 catcattgaa aagaaaataa aacctaaaga cacctgggga atgtcatttg gctaaatgta
    59041 tgccgctatg ttaaaatgag agggatttgt aaaaaggaga cgaaaatatg attactttaa
    59101 aatcagcacg tgaaattgaa atgatggcag aatctggagc tttattagca gatgtacata
    59161 aaaatctgcg tgattttata aaaccaggaa tcacaagttg ggatattgag gtatttgtca
    59221 gaaactatat cgagagtcat ggcggaattg ctgcgcaaat tggctttgaa gggtatgaat
    59281 atgcgacttg tatcagtata aatgatgaaa tttgtcatgg attccctcgt aaaaagcctt
    59341 tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga tttgaaaggc ggcatttctg
    59401 attcttgttg ggcctatgta gttggagaat ctacacctga gatcgaccga ttgatggaag
    59461 taacgaagaa agctttgtac atcggtattg agcaagcaca agtagggaat cggattggtg
    59521 acatcggtca tgctatccaa acttatgtag aaggcgaaaa tctagcgatc gttcgtgatt
    59581 ttattggtca tggcgttggt cctacgattc atgaagaacc agcagttcct cattacggag
    59641 aagctggaaa agggttgcgg ttgaaagaag gcatggttat cacgatcgaa cctatggtta
    59701 ataccggaac ttggaaaatg aaaatggatc ctaacggctg gactgcgtat acaagagacg
    59761 gcggactttc ttgccaatat gaacatacat tggctattac taaagatggt ccaagaattt
    59821 tgacatcaca aggagaagaa ggcacttatt gatctatcca gtcaataggt acgggagagg
    59881 tacatgaaag tttggaacaa aataaaacaa aatgaaaatc tgatgcgctt tattgagacg
    59941 actcaaaagc gcatcactga ttcagaaatg agtacgacct ctgtcgtcgt tgcttattat
    60001 ttactcttgt ctttatttcc attgattatt gcttttggta atattttacc gtttctccac
    60061 atcgatcaag agactgtact gacctattta cgagaagtga ttcctgagac cgtttaccag
    60121 tttatcggtc ctgcaatcaa agaccttttg acacaaagtt caggaggctt gttatccgtt
    60181 tctgcattag cggctttatg gtctgcaagt caaagtatca atgctttgca aattgcgatg
    60241 aataaagcgt atggcgtaga aaatagaaaa aattttttga tcgtccgttt cttctcacta
    60301 attgtcattg tcttgttcat gattgctatt agtggggtaa ctatcgttct tgggttggga
    60361 cagctgattt tggatgctat tcagccaatc gtacaaatac ccgtgaactt tatcgatcag
    60421 ttccaaactt tgaagtggcc aatcacattg gtagctttat ttgtcatcat gttcttgatt
    60481 tatttaattg tccctaatgc tcagttgaag ttgaagacga tcattccggg cacgatcttc
    60541 gctactgtcg gctggatgct gttatcacaa gtgtttagta tctatgctag gtattttgca
    60601 acgaggatca gcggttatca gatcatagga agttttattg tactcatgct ttggcttaat
    60661 tttgctgcta cgatcatcat tttgggcgga atcatcaatg cagttgttca agaatacgtg
    60721 acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa aatggttcaa taaattgaaa
    60781 actaaatttt ctcataaaaa aaagtagaaa caagagttct tattgtaaat ttatatcagt
    60841 aatttataat aattatgact cttaattaaa gtaggtgccc atatgcttgt ttcttttagt
    60901 atggttataa gattttttct gacaatttta ttatcactga tattaacacc tgtattcaaa
    60961 atcatttcag tacaaacagg tatggtcgac aaaccaaatg aaagaagaat aaataaagtt
    61021 ccaatgcctt cagcgggcgg actgcctatc tttatttcat ttgttatatc gactttattc
    61081 ttattccgta aaattatccc tcaatcgtat attgtaccga tcttgattgc ttctttagta
    61141 atcatagtca caggagtgct cgatgataaa tatgagctga cacctaaaca aaaaagtata
    61201 gggatcttga ttgcttctct tattatttat tttgtagcag atatacgtat tgattccttc
    61261 acgcttcctt ttattggata tatccaacta ggctggctta gttttcccgt aacgattttt
    61321 tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg gtttggatgg gctggcggca
    61381 gggatttcat tgatcggttt aattacgata ggaatcattg gatatttttt cctccatgct
    61441 tctacagtgt atattccagt tgttattttc tgtcttgtag caagtattat cggttttttt
    61501 ccttacaatt tttatccggc aaagatttat ttaggcgata caggagctct attcctaggg
    61561 tttatgatgg ccgtcctttc actacaaggg ctgaagaacg tgacttttgt ttcatcgatt
    61621 tctttgctga tcgtcatggg agtgccggta acagacacgt tttttgctat cattaggaga
    61681 aaagcaaacc gtgtttcttt ttctacagca gataaaaaac atcttcatca ccgactaatc
    61741 agtttaggct ttactcataa aggtgctgtc ctgacgattt atgcgatggc tttgatgttt
    61801 tctttcacag ctatggtcat gaattatact ggaagaatcg gaacgatcgt attgattatt
    61861 gccatgttgt ttgctgcaat tttattattt gaattgattg gtctgatcaa tgagaaacat
    61921 cagtttattt taaaaacatt gcgattttta gggaataaag agtaccgtac gcaagtaaaa
    61981 cagaaatatt tgaagaaatg gaaccgtcat atagatgaat aacaaattaa accagccgat
    62041 ccttattagt attgtgacgg caaacagtga taaaattttt cggacactag ataattttat
    62101 ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt tttgacaaca attcacttcc
    62161 cgagtacaaa aaaagactaa cagagtatag gaaatatccc tacatcgata tctattttca
    62221 tgatgaaaat tccggatttg gttatggaca taatcacaat ctgatagcta gcgattctcg
    62281 ctatggcatt atttgtaacc cagatatttt agtggatgaa gagacagtta cagcattggt
    62341 cttgttattg aaagaacatc cggaatgtgc gatgttagca cctaagattc ttaatgaaga
    62401 tggaacaact cagcacctca tccgtgagca gctggctgtt tttgattacg ttttgcgctt
    62461 tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga ctggctgtgt ttgaatgccg
    62521 aggattgcct actgatgcta atagttatgt acggatgatc tcaggtagtt ttatggtagc
    62581 tgatattgat aaatttaaag agataaaggg gtttgatgag cgtttcttca tgtactttga
    62641 agacaacgac ttgtgtttga gttttgaaaa agcaggttac aagttattgt acacaccctt
    62701 ttatgcagtt atccattttt acgggaaggg tgcgcatcga aatgcaaaac tgtttcgcgt
    62761 gtttttgact tctatgatga aatttttcaa taagtggggg tggcgttttt tctgatgaaa
    62821 ccagaaatat tagtagtcgt tgtgctttat cagcaactat tcagtcaatc tccagcttat
    62881 ggaccattaa gcaaagcact tagcgaaaag caaattcagc ttattattta cgataatagt
    62941 cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata tcgtttatca tcatgatccg
    63001 caaaatcctg gtttagcggt ggcttataat tttgcgttgt ctcaagcaga tgaaggaaca
    63061 cgttgtctcg tcactctgga tcaggatacg aggctagtcg acgattattt tgaactattg
    63121 cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc cgatgatttt ttctgggagc
    63181 cgacaagttt ctcccctata ttcagatcgt tatatcaatc gtacagctca agcagtagaa
    63241 gttgaaggcc caacagctga acgaatcatg gcaatcaatt caggtgttgc ctggtctgtc
    63301 aaatttctaa aaaagattgg cgggtttaat cctgcttttt ctttagattt tttggatcat
    63361 tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg agatccttcc tgtacgaatg
    63421 gaacatgatc tttcggtttt agactatgaa aaagtttcaa gtaatcgata ccgttctatt
    63481 ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa ggcatttatc cagtcaccga
    63541 cgacaattat tgttaagaac agctaaacag ttcttgaccg tcaaaaaccg gcatatctgg
    63601 cggatgactt tgcgctcata tgttaagaat tggaaggtgt gaattcgtgc gttcggtttg
    63661 tatagctacc tttaatggag gcagatattt aagagagcag ttagatagta ttttatctca
    63721 gctttcagca gaagatgaag tagtcatttc tgatgatggg tcagtagatg atacatggga
    63781 tatattaact gaatacgcag aaaaagataa gcgaatccag cttttttcgg ggcctcgtca
    63841 aggattgatc gctaattttg gttatgcgat ccagcagacg aaaggagatc taatcttttt
    63901 ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa aagatcactg actattttga
    63961 gcagaatccg gatcagctag tagtagtttc tgatttaatc attgtggaca gcgagctgca
    64021 agagatccat gattcgtatt tttcgtttcg aaattccaaa gaaggatggc ttaataatgt
    64081 gataagaagt cactatattg gtgctggtat ggctttccgg tcttctttaa aacccgatat
    64141 tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg attggtttgc tgggaggaaa
    64201 gaagactggg ttcctaagag aaccgttaac tctgtaccga agacatgatt taaacgcgag
    64261 tgagattgaa acacattcta aattacggca aaaaatttct tggcgtgttc atctgattac
    64321 ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag atgtttaaat ccttaatatt
    64381 tccatgacaa atataacaat tgatgagata ataacaaagt gaaatttgaa atcaaaaagg
    64441 agagcagata tccatgaagg gaatcattct tgctgggggc agcggaacac gtctgtaccc
    64501 tttaacaaag gcaacatcga aacaattgat gccgatttat gacaaaccaa tgatttatta
    64561 tccaatgtct actctgatgt tggcgggaat caaagaaatt ctgattattt caacgccaca
    64621 agatacacca cgtttcaagg aattatttgg aaacggaaat gatttaggac tgcagatcga
    64681 atatgcagta caagaaagtc cagatggatt ggcacaagcg tttatcattg gagaagaatt
    64741 tattggagat gatagtgtct gcttggtatt aggggacaat atttactatg gtggcggtct
    64801 gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc gcgacagtat ttggctatca
    64861 tgtcaatgat ccagaacgct ttggtgtagt agaatttgat gagaatatgc aagctttgtc
    64921 gattgaagaa aagcctgctc agccaaaatc aaattacgct gtaactggcc tttattttta
    64981 tgacaatgag gtaatttcta tcgctaaagg tatcaaacct tcagaacgtg gagaactaga
    65041 gattacggat gtcaataaag catatttgga taaaggaaaa ctatccgtag aacttatggg
    65101 acgcggattt gcttggcttg atacaggaac acatgaatca ttgcttgaag catctacctt
    65161 tatcgaaaca attgaaaaac gtcagaactt aaaagtggct tgtttggaag aaattgctta
    65221 ccgtatgggc tacatcgata aggaacaatt gctggccctt gcacagccat tgaaaaaaaa
    65281 tcaatacggt caatatttat tgaatttggc cgctgaataa ggagtcctta cagatgaaag
    65341 taacagatac aaaattaaca gatgtcaaaa tcgtagaaat ggacgtcttt ggcgatcatc
    65401 gcggtttctt cacagaaagt tattcaaaag caaaatttgc agaacatgga ttgaaatatg
    65461 actttgttca agacaaccat tcactatcag ctgaagctgg tgtccttcgg gggcttcatt
    65521 tccaaaaagg agaagctgca cagacaaaat tgattcgggt agtcactgga gcagttttag
    65581 atgtagtagt ggatatccgc aaaggtagcc cgacttatgg tcaatgggac ggatttatct
    65641 tatctgaaca taatcatcga cagttgcttg tacctaaagg atatgcccat ggattcgtta
    65701 ctttgacacc aaatgtgaac ttcctttata agtgtgataa ttactataat gcagaagcag
    65761 atgggggcat cgcatttgat gatccagatt tagcgattga ttggccaatt gatccatcaa
    65821 gagcgattat gtcggaaaaa gatcaccgtc atccgacatt aaaagaattt gaagcagaaa
    65881 atccatttgt ttacggagaa atctaagaat taaggagaaa ttttcttatg aaaaacatca
    65941 ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt tcattacgtg gtaaacaatc
    66001 atccagaagt gcatgtcact gttttggata aactgactta tgcaggaaac aaagagaact
    66061 tggccggact tcctagcgat cgcgtagaat tggtcgtagg agatgttgca gatgcagaat
    66121 tagtggaccg tctagtaaaa gaagcggacg cagttgttca ttacgcggca gaatcccata
    66181 acgataattc attaaaagat cctttcccat ttgtacaaac gaatatcatt ggtacgtata
    66241 cacttttaga agcttgccgc aagtatgatg tacgctatca tcacgtatct actgacgagg
    66301 tttacggcga tcttccatta cgtgaagacc tgccaggaca tggggaaggc gaaggagaaa
    66361 aattcactgc ggaaacacca tataatcctt caagtccata ttcttctaca aaagctggtt
    66421 cagatctgtt agtaaaagca tgggtgcgtt cttttggctt acgtgcaacc atttcaaact
    66481 gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt tattcctcgc caagtaacga
    66541 gtatcttaag cggtattcgt ccaaaattat atggcgaagg aaagaatgtt cgtgactgga
    66601 tccatacgaa tgatcattct tcagcagtgt ggctgatttt gacgaaaggc agaattggcg
    66661 aaacctatct gatcggtgca gacggtgaag aagataataa gaccgtgatg gaattgatct
    66721 tagaaatgat gggccaacca aaagacgcat acgaccatgt aaacgaccgc gcagggcacg
    66781 atttgcgtta tgcaatcgat tcgacaaaat tgcgagaaga gctaggttgg cagcctgagt
    66841 ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg gtacgaagaa catgaagact
    66901 ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc caaaaatgga caataataca
    66961 gaggaaattt cactgtatta atggaaaaac acatgtgagg caggcgctcg cctgtctctt
    67021 ttttaaaata aaaatcaagg agcggataac catatgtttt tattaactgg aggaaatgga
    67081 caattaggta cagaattgcg ccatttatta gatgaaaaaa atctgccata tgtttcaaca
    67141 gatgcaaaag acttggatat cacagatgct gagaaaacaa tggcttatat tacagaatta
    67201 aaacccgatg ttattttcca ttgcgcagct tacacagcag tagataaagc agaagaagaa
    67261 gcaaaagaat tagatgagaa aatcaatgtc gatggcacaa gaaatgtagc acttgcagca
    67321 aaagctgtga atgcacaatt cgtctatatc agtacagact atgttttcga tggaaaaaac
    67381 aaaaatgtag aatacgaagt agacgaccaa acgaatccac taaatgaata tggtcgtacg
    67441 aaattattag gcgaaaaagc agtagaagag attttagaag actattatat catacgaact
    67501 tcttgggttt tcgggatcta tggacacaat ttcgtcttca cgatgcaacg cctagcggaa
    67561 acccgcgaca aactgactgt agtaaatgac cagtttgggc gaccaacttg gacacgtact
    67621 ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac catttggtat ttatcatttg
    67681 tcgaacgaaa atagctgttc ttggtatgag ttcgcaaaag aaatattgaa agatacagat
    67741 gtagaagttg cacctgttac gtcagaagaa tttccgcaaa aagcaacacg tccgcagtat
    67801 tcagtcatga gcctgaaaaa gacagaagcg ttaggttttg tcataccaac ttggcaagaa
    67861 gcacttgcgc aaatgctgga agctgccaaa aaaatgaata agtaatttcc tttttatcaa
    67921 atagaatttg gagcatgata tggaaaaaca aacaagaagt gcacgacgaa aagaaaagcc
    67981 aaaagcgaat caggcagatt cgaatttggt gcttgacctg tttctaaaac tccgctatct
    68041 tattggcgtt ttagtcatcg tggtcatcgt tacttttaat ttaaacggaa gttcacttgg
    68101 tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa aaaactgatg tgatttttgg
    68161 acagaatcgg gaagtgcggt cagacgaatg gctggtgcag acaccttttt acctctcaca
    68221 agctgaagag gacttttcgt tggtgaacga ggattattca ttaaacggac aaaacatgat
    68281 cattgcgtat aattctcctg taaaggatat tacggttatc ggaaaaccat tcaactgggg
    68341 attctttttc ctcggaagag atcgaggact ttccttttat tgggcgatga aattagtcgt
    68401 catggtcttg cttggattcg aagtactgat gatattaacg aagaggaaaa aagctctatc
    68461 gctaatcggt gcatttgcgc tgacattttc tccagcagtc cagtggtggt tcatgcaaca
    68521 tgtgggcgat ttgattttct ttactttggg actgatgatt gctttttatc attatttcta
    68581 caatcatgat aaaaaatggc tacgaacgtt gatgatgctt ttggtggtta ttttcggtat
    68641 tgggtttatt ttagtgattt acccagccca tcaagtgatg ctggcgtact tgctgatttt
    68701 ttattttgtt ggcctgctta tttactatag caagaaagtt tcatgggatc tgttggatgt
    68761 agtgttgatt ggaggggcag ttttatttat tggcggagtc atggttcatt tctggctaac
    68821 gtcaaaagac gcattgttag cttctttgaa taccttatat ccaggaaaac gggtaagtac
    68881 tggaggaaag tggacgattg gcgaattttt ttctttcctt acaaattgga aaattccttt
    68941 tgaagatatc aactattcga ataactctga agtggccctg ttctatcact tttttccaac
    69001 tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa gatagcgaac aaaagttact
    69061 gggacgtatt ttgatgattt tttgtctgtt ttctatcttt tggattaccg ttgggttgcc
    69121 aaaatggcta gcagaaatca ctctgctgtc ttatgttcct ccattaagag ccatccttac
    69181 ttttagtttt gcggcttgtt tattatcgat ctggtttatc gcttatattt ggagagaaga
    69241 tgttattcct atttggtata aattcatttt gttggcagta aatgcatggt cttatttcta
    69301 tgcgattacg cattcgaaaa tggataatta tttttcggat tttgaaacca tcgcagttgc
    69361 tgtgttggga acagtgattt tagcttttgc attatttggc tggagacgct tgttttacct
    69421 cgcttttgtc gccttagtta tcgtatctgg ctttttcgtc aatccagtcg tagaaggtac
    69481 aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa aaaatcgatc aacaagatcc
    69541 tggtaaagtt tggttgtctg aagatgaatt atataacttc accccaacat tgggagtgaa
    69601 atccttcaat acagtacgct tttatccaga tatagctgca tggaagaaaa tcgatccgaa
    69661 agaagaatac gagaagtatt ataatcggta tgctcataca agagctttta tcgctaagga
    69721 agatacaaag ttcattttag atcgtccaga tatgttttcg gtcacgttga attttgaaga
    69781 cgcagaaaaa ttagggatcg actatgtgat cagtaaacga ccattaactg attacaacca
    69841 attatatgaa gcgcagttca cgcagctata tggaccggat aaagacggtt atatgatttt
    69901 taaagtagaa tatccggatt atccagatgt accacaaaca caaactcagg aaaatcagct
    69961 agtcgtacag taataggcta gctgagaata atcctgaaat attagtagaa aggagagtga
    70021 agataattca agcaatcgta caaaaatttg tcaacaaaat gagtgtcggt ttcttgcttt
    70081 tgttgctgac gctagtcttt tttgtttgga cagcaggggt aggttttcca gcaactttgt
    70141 ttatatataa caatagttca ttacttatcg cttttagtgt ggctattttg ctcattttaa
    70201 atgtttataa agcaaaaggc gtcgattttc tttatctagg tgttgcgctt gcccttttca
    70261 ttttcttttc ttactttcgg gaattaagaa atgagtcttt atttggagac ttactgtttc
    70321 ctttggttat tttattttgt ttagtaatta aatggatcga gtggacaaaa tgggatcgcg
    70381 gtatttatct gattgtcttt acggtcttcc tcggaatcac gttatatcgt gtatttacag
    70441 agatccaagt accagaagga caaagcatct gggctccagg caattcgctt tctgatattt
    70501 ggatcaatac gaatacaatc ggaagttctt tgatgactct tgctttattg atcagtggat
    70561 ttgccagttc ttttgaacga tggtatgttc gaatcatcgg tgggatagca gtcgttgcag
    70621 cctttttagg tatttgggtc tgccaatcta gaggtgcatt agtcgcgttg attgtttttg
    70681 cgatattgga tcttttacct aaatcgttga tgcgtgtcat tcgtgcgcca tttattgcct
    70741 acacggttac gattctttta gctttgccga tttcttatct ggcggctgtc tctgaaaaag
    70801 tcaatttgtt taccgatcga gaagatattt ggcataaatt ctatcagacg ctaggagaaa
    70861 aatctgaaca agttttacta ggaatgaaaa catttatctt ccagcgagga aaccaatttt
    70921 taggaaacca caatagttat aattctattt tgaatatcta tggattgata ggatttggga
    70981 ttgctgcttt gctgcttatc ctttttattg gtcgcttaac gttgaaagca gatttatcca
    71041 acgggcagat gacttttatc tgggcctttt ttgcggtgat gatgcagtca tttatggagg
    71101 atacattgac ttcataccca tgggttccga ttatttatat cctgttggct atggccgttc
    71161 atcgatatga tgaaccagaa gttgcggagg aattctatgt tatggaagag gcatatgacg
    71221 atatgactta tgatgataca tcttatgagg aacaaccaga tagcgctcat tcttccagga
    71281 tccaacgcta tcattgatag gcacaaaata cgagagaggc tgtgacaacc gtcgcagcct
    71341 tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta gagaaaatct
    71401 tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa atatggtaaa
    71461 atggcacttg actgtaattc actgaaaatc gcttggagga acacaaatgt ttaaagattt
    71521 gtatttattt atgaaggaca tttatcagaa cagaaaatta ttgctacagt tttcgatcaa
    71581 tgacttcaaa tcaagatatg caggatcgtt tttaggaatc ttatgggcgt ttatcaatcc
    71641 gctatttact gtattgattt attggctggt atttggtgtt ggactgaaag cacaaatgat
    71701 cgatgcaaaa tacccattta tcgtatattt agttaccggg atgattccct ggttcttctt
    71761 ctcagatgca tttgcatcaa ctacggttgt ttttcgggaa tatacttatt tagtaaagaa
    71821 agtcgtattc aatattcgta tcttgcccac gacgaaaatc ctatccaatc tgtacaccca
    71881 tttattcttc attctgattg gcatagctgt cgcgcttata aatggtatat atccttcagc
    71941 aatgtcttta cagctgattt attatttatt ttgcatgtgt gctttcttga caggattgac
    72001 ttggctgacg gcatcgatcc aaccattttt acctgatatc atgcaattaa ttacgatcgt
    72061 gctgcagttg atcatgtgga cattaccgat attatggagc cctaatcagt ttgattcagg
    72121 tcttgtaagc atattgaaat taaatccgct gtactatgtt gttcaaggtt atcgtgagtc
    72181 atttttaagt gaagcttggt tctgggagca tggaacatat actttgtatt tctgggtttt
    72241 cacgttagta atgttcgtaa tcggctctgc agtgttccgt agattgaaac cacatttttc
    72301 agatgtgctg taaggagaat agctatgaca gaatatgcaa ttagactaaa agatatcaca
    72361 aaatcttata atatgtacgc aaaaccttcg gatcgatttc gtgaagccct caatcctttt
    72421 aagaaatcct atcatgatgt attttatgcg ttgaaaaacg tgaatgtaga aataaaaaaa
    72481 ggtgagatga tcggtttcgt gggagaaaac ggatcgggaa aatctacaat gttgaaaatc
    72541 attacgggag tactgacacc aacatccggc acgatggaaa tcgaaggaaa aatctcagct
    72601 ttgctagaac taggatcagg attcaatcct gagtattctg gctatgaaaa tatctattta
    72661 aatggaatgg ttctaggatt ttcaagagaa gaagtcgatg aaatggtcga tgatatcatt
    72721 gaatttgctg acatcggaga ccatatttat caacctgtaa aaacttattc aagcgggatg
    72781 ttcgttcgtc tagcttttgc tgtagcaatc aatgtgaatc ccgatatttt gattgtggat
    72841 gaagcattgg cagtaggcga tcttgaattt caactaaaat gtatggaaaa atttacagaa
    72901 atcaagaact cgggaaagac gattttattc gtttctcatg atatcagttc gattcgtcgt
    72961 ttttgtgatc gttcctattg gttgaaaaac ggagaagtcg tcgaatatgg cgatacgatg
    73021 gaagtaacga cgaattatga aaacttttta aagaaaaaat cagttaaaac agtggatcgg
    73081 aataaaaacc aagtagaacg gttcgccgct ccagatatcg tcgacgtgac gaaagcggaa
    73141 ttgttggata aagacaaaaa tccggtggat atgctggaac aaggagaaaa actatacgtt
    73201 aaagtaactt atgaagtaaa agatgataca atcaaaaatc cggttttagg aattgcgcta
    73261 cgaacagtcg acaattttta tgtatgcgga cttaatactc tactagatgg tatcaagatt
    73321 ccttggaaaa aaggagagaa tatcttttat ctggagtatg aaaagttagg tttgttagct
    73381 ggtgaatatt attttgacgt tgcggtcttt gaagaaaatg caacggttcc tttggtatat
    73441 aagacaaagt acatgaactt gtttgtcagc ggttcttata tcggagaagg catcgtggtc
    73501 ttagaccata aatgggaaga agggactcat agcaatgaaa tatgattttg aaatggacct
    73561 agatgaacaa agcagtgtgg gaaaaattgc cgcacagatc aaaccaggaa gtaaagtttt
    73621 agagtttggt cccggaaacg gccgattaac gaaacattta atcggagcaa aacaatgtga
    73681 agtgagcatt gttgagttag ataaggaatt gttcgatttt gtcagcgaat ttgctcaaga
    73741 tggattttat ggtgatatcg aaagttttga gtgggcaaac tattatgctg gacagacatt
    73801 tgattacgtc ttgtttgcgg atgttttaga acatttggta gatcctggaa aaacattgaa
    73861 gaaagtcaga gaatttttga atgaagaagg agagatcctg attacattcc ctaacttggc
    73921 gcataattct gtcatgatcg atttgttcaa taaccaattg ccgtgggctt cttatggtct
    73981 cctagatgaa acccataatt ctttttatac tcatgatggc ttccaaaaag tttttgaaaa
    74041 agcgggatta ttcattaata ttgaagatta tttgtactta gctgtaggtg atacagaatt
    74101 aaaatcaact tatgaagaat tgccggaagc tgtacgctat gatttcaaaa tgcgaccatt
    74161 tggtgaagtc taccaatact ttttctcatt gatgaaacat ccagtagcac aaagttccat
    74221 tgcagagcca caaaattcta attatgtaaa agtgttagaa gtaacccagc aaacgaaaca
    74281 agatgaaaca atccaacaaa taccattcaa taatttcaca ggggaaaatg agacactaag
    74341 tttccccgta tcagaaacaa cagaaagaat ggtgtttcgc ttcgcgcaac aacctagctt
    74401 tatcgaattc agcgcagaag ctgccggaga aaaactaaca tttatcgatt cgaatgctgt
    74461 agtcaaaaca gtaaatgatt gttatttgtt tgatgggaaa gaattaccag agttcgttct
    74521 aacggatatt tctgggaaag aagtgactat ccattgtcac tatcgcttta tcggggaatt
    74581 gacaccaaca atgaaagaat tgttggaaac gattcgtccc atggctgaag tcacaaagca
    74641 gctttctgag aaaaatgacg aattagaaag agaaaatcat catcttcttg aggaaaatac
    74701 aagactagac catacgttaa aaacaaccac aaatcgctat tgcacattgt tggaaagtga
    74761 tgaatggaca ataaaacata gactaggacg acgtaagaaa gaaacttcga aaaaaatcca
    74821 agaaaaagaa ctttctatat gtattgacga gaaaatctgg gatgcagaaa caaaaatatt
    74881 aaaaatcatc ggatggggaa tcgctaattc ggatcgccag cccttgtcct ataagttatc
    74941 agctgaccaa tcgccatttt tcgaagcaat tcctgtttca cgagaagaag tcaatcaagc
    75001 agaacagctc gcaaaaggaa cagaagcggg atttgagtta cgaatccttt gcgagcaaga
    75061 acgttcattc ttagtggaag cagtagctca aaatggccaa tcatggatca ttgaaatgta
    75121 aataatgatt aacttataaa gataaggagt aaacgaatcg tgcaaaaaaa agaaattgcc
    75181 gtatttatcg atcaaatcac tcgtgaacgg gccactggcg atttgcttat tgtgggctgg
    75241 gcaattgatg aagtgaccaa agaaatccct acgataaagg tagaaaaaga aaatgtgatt
    75301 gctgaagcaa cacatgtcgt aagattggat atcaatcatt tatataatct tgatgttaag
    75361 acccaaagcg gtttcaaggt acgtttatca gggaaaatga gaggaaaagc catcttagac
    75421 ttccaaacag cgagacatca aaatggcata gctgtgaaat taaacggtaa atacccttat
    75481 gatgacggca ttgaatcttc atgggaaaga aaaaaaagat tactgaaaaa agggataaat
    75541 tatgcaagga ctcatggcgt aaaaaaagcg atccgtcggg tcaaattgga attgaagcca
    75601 ggatcgatcg actatgcaga gtggatcagt cgtcatgaga aaccagattt gaaagagcaa
    75661 aggaaagaag cacaaggatt tgcgtaccgt ccattgattt ccatacttat gcctgtctat
    75721 aacgttgaaa tcaaatggct cgaaaaatgc attgattcgg tactggatca aacgtatgat
    75781 cattgggaac tatgtatcag tgatgatgct tctactgatc cagctatccg aaagtgtctg
    75841 gaatcctatc aagcaaaaga tgatagaatc aaagtcgttt tccgaaaaga aaatggacac
    75901 atcagtctgg caactaattc tgcattggaa atagcagaag gagaatttat agcactctta
    75961 gataatgatg atgaattacc gccatttgca ttatatgaag tggcaaaagt gctaaacgta
    76021 catccagaac tggatgtgat ctacagtgac gaagataaaa tcgatgcaga tggcaatcgc
    76081 tttgatccgc atttcaaagc agactggtcg cctgatacat tgatgggaaa taattatatc
    76141 tctcacttag gcgtatatcg aacaagcatt gttaaagaac ttggcggttt ccgtaaaggc
    76201 tatgaaggtt ctcaagatta tgatttggtc cttcgtgtga cagaacaaat ccctgcagat
    76261 catatttacc acattgaccg agtcttgtat cattggcgga cgatcccagg atcaactgca
    76321 agtaatggcg aagccaaaag ttatatctat gattccggag tgaaagcatt gacagatgcc
    76381 ctgtctagaa gaaacatcaa aggaagcgta cgtcctggca gaatttctgg attttatgag
    76441 attgcttatg acgtcttgca agaggatttg gtcagtgtga tcattccaac gaagaatggc
    76501 tatgaagatc taaaaacttg tgtggattcg atcatcgaaa aaacgtctta ttcaaattat
    76561 gaaatcatta ttgcggataa cggaagtaca gatccaaaaa tgcaagaatt atttgcagaa
    76621 tacaagcatc agctgaaaga ccgctttatc gtagaattga ttgacattcc attcaattat
    76681 tctcgaatta acaatttagc agcagaaaaa gcaaacggca aatacttttt attcttgaat
    76741 aacgatacag aagtgattga gccagattgg atgacggtga tggtttctta tgcacagttt
    76801 gatcgaatcg gctgtgtagg agcaaaactg ttttatccag atgatacaac gcagcatgca
    76861 ggagtattat taggaatcgg tggtgttgca gggcatgcat tgaataacta tgatcgaaca
    76921 cattgtggtt atttcggccg attggtcatt gacgtcaatt acttagcagt cactgcagct
    76981 tgtatgatgg tcaaggcagc tgacttcaat gctgttaacg ggtttgatga aacattagaa
    77041 gtagcattca acgatgttga cttgtgcttg aaagtttatg aactgggtcg ttacaatgtc
    77101 tatgcacatc aagcagaatt gtaccatttc gaatctaaat caagaggtta tgaagatact
    77161 ccagaaaaac aaagacgttt tgctggtgaa atcaaaaaaa tgcaagataa atggccagca
    77221 tatattgcgc atgatccatt ttacaatgat aatttaacaa aagacggaat cggcgacttt
    77281 tcgttgagat cagattaaac tataataaaa agctgttatg ttgtgattcg ctcgcacata
    77341 acggcttttt atagtttttt gtcaaaaaaa ggcagaaggt gccgactcaa aaaaataagt
    77401 cgaggttggg acagaagtgt ttaactccga gaaataagaa gaaattcacg aaaattgctt
    77461 ttcaaatttt tgtgaatttc ggcttattta agaagaagtt gcttctgctc ccaccgttta
    77521 ttcgttttta aagaatgaga caaaagtgtt tcttactttt gtcccacgct cttattcatg
    77581 ctcagagcgc caatagatac aaaatcatag ccaaagtttc acgaacccta gctgtcaaag
    77641 aatcgatatt ttattttaat ataggctatt ttaaaataaa tggtttaagg tattcctcta
    77701 caaaagccac tctatcaatt atcatgggtt taaaataaga agtgatggag ataaccagat
    77761 tgttcttagc gtcaatataa agaacatttc cactattacc aattgctgaa tatatttcgt
    77821 tctcagaatc taatacccac cataaatagc catatctcat attttgtaag tcttctaaag
    77881 cactggttct aggtttgaac atctcgttta tccacatggg tggtacgatt tgttttcctt
    77941 tatacatccc tttatttagg cataatacac caatttttgc cagatcactt gccgataaac
    78001 acaaaccata tcctgccgtc ccgatattta gtggatcaca aaaccagaca ttggttttag
    78061 gagacttatc cattgtaaac tgcttatgtt cttcagctgt ttcagcataa taattgacat
    78121 ggtttggtat ccctatagga tcaaatagat acttattggc aaaatcaaca gttgttagct
    78181 tacttgtctg atacaacaag ccagttaata tgtgtaaaca gacagtttga tagttgaatt
    78241 cgccggttat accttttctt cctcccaata aatctagtga tgtttttgtc caatcttcac
    78301 ttgaacagac ttttgtccat ggatcgcctt tacatttgta gggtgctttc attgttagca
    78361 agtgcttgat tgtaatagcg taaatagttt tctccccgcg tttcacttta tactctggaa
    78421 aataatctaa tactttatca tcgatacttt tgattaatcc ttgctctaaa gcaatgccaa
    78481 tcagcaaaga tacaatactt ttcgttgctg acatgatatg acaagtatcg tcttttttat
    78541 aaccattcca ttcatctgta taaacttcta ccccatcctt atatgcgaca atttgacata
    78601 tatttggttg tcgaaaacca ataaaatcat gcaactcttg tttattcata agatattacc
    78661 tcactttctt ctataagcat aatgtcatta aaaatcgatt tcaatttttt cgattcttca
    78721 cgtaatacag gtattcttat ggactagaca gcgctttggg aagatggatt ctctgaaaaa
    78781 tataaaggtt cactttatga gtggataaaa aaagcagcaa atacttcgac ttctttaatt
    78841 taccaacatt agtgaaaatc gattcatctg gagatttgta atttagagtc cttgtcttga
    78901 ttagaggtgt ataaagcttt ttattagaca tttttaaatt ccattacaat ttgcttataa
    78961 actaggtttt taaaggtgac tatggtaaga taatagagat attattttgt gattaggagt
    79021 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
    79081 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
    79141 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
    79201 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
    79261 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
    79321 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
    79381 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
    79441 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
    79501 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
    79561 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
    79621 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
    79681 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
    79741 attgaagcat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
    79801 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
    79861 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
    79921 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
    79981 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
    80041 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
    80101 gtaggaccac gtcctgaacg ttctttcttc gtcgatcagt tcaatgcaga aaacccttat
    80161 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
    80221 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
    80281 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
    80341 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
    80401 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
    80461 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
    80521 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
    80581 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
    80641 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
    80701 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
    80761 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
    80821 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
    80881 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
    80941 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
    81001 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
    81061 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
    81121 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
    81181 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
    81241 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
    81301 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
    81361 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
    81421 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
    81481 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
    81541 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
    81601 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
    81661 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
    81721 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
    81781 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
    81841 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
    81901 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
    81961 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
    82021 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
    82081 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
    82141 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
    82201 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
    82261 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
    82321 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
    82381 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
    82441 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
    82501 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
    82561 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
    82621 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
    82681 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
    82741 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
    82801 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
    82861 atgagaaata tttaaaatat gtattatta
//
LOCUS       DACDTH010000006        70961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDTH010000006 DACDTH010000000
VERSION     DACDTH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.006"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            162..392
                     /locus_tag="IWK54_000456"
     CDS             162..392
                     /locus_tag="IWK54_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9592606.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="IWK54_000457"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="IWK54_000457"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9592607.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="IWK54_000458"
     CDS             1803..1979
                     /locus_tag="IWK54_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9592608.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="IWK54_000459"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="IWK54_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9592609.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="IWK54_000460"
     CDS             2474..2941
                     /locus_tag="IWK54_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9592610.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="IWK54_000461"
     CDS             3026..3910
                     /locus_tag="IWK54_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9592611.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4027..4260
                     /locus_tag="IWK54_000462"
     CDS             4027..4260
                     /locus_tag="IWK54_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9592612.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="IWK54_000463"
     tRNA            4348..4428
                     /locus_tag="IWK54_000463"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="IWK54_000464"
     tRNA            4435..4506
                     /locus_tag="IWK54_000464"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            5021..5728
                     /locus_tag="IWK54_000465"
     CDS             5021..5728
                     /locus_tag="IWK54_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9592613.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="IWK54_000466"
     CDS             complement(5788..6432)
                     /locus_tag="IWK54_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9592614.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="IWK54_000467"
     CDS             6559..6846
                     /locus_tag="IWK54_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592615.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="IWK54_000468"
     CDS             6954..7274
                     /locus_tag="IWK54_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9592616.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="IWK54_000469"
     CDS             7355..8668
                     /locus_tag="IWK54_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9592617.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="IWK54_000470"
     CDS             8665..9384
                     /locus_tag="IWK54_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9592618.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="IWK54_000471"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="IWK54_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9592619.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10285..12819
                     /gene="secA"
                     /locus_tag="IWK54_000472"
     CDS             10285..12819
                     /gene="secA"
                     /locus_tag="IWK54_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9592620.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12945..14046
                     /gene="prfB"
                     /locus_tag="IWK54_000473"
     CDS             join(12945..13007,13009..14046)
                     /gene="prfB"
                     /locus_tag="IWK54_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9592621.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14151..14837
                     /gene="ftsE"
                     /locus_tag="IWK54_000474"
     CDS             14151..14837
                     /gene="ftsE"
                     /locus_tag="IWK54_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9592622.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14821..15714
                     /locus_tag="IWK54_000475"
     CDS             14821..15714
                     /locus_tag="IWK54_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9592623.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15866..17314
                     /gene="cls"
                     /locus_tag="IWK54_000476"
     CDS             15866..17314
                     /gene="cls"
                     /locus_tag="IWK54_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9592624.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17651..18508
                     /locus_tag="IWK54_000477"
     CDS             17651..18508
                     /locus_tag="IWK54_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9592625.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18623..19543
                     /gene="pstC"
                     /locus_tag="IWK54_000478"
     CDS             18623..19543
                     /gene="pstC"
                     /locus_tag="IWK54_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9592626.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19543..20427
                     /gene="pstA"
                     /locus_tag="IWK54_000479"
     CDS             19543..20427
                     /gene="pstA"
                     /locus_tag="IWK54_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9592627.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20442..21248
                     /gene="pstB"
                     /locus_tag="IWK54_000480"
     CDS             20442..21248
                     /gene="pstB"
                     /locus_tag="IWK54_000480"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9592628.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21264..22022
                     /locus_tag="IWK54_000481"
     CDS             21264..22022
                     /locus_tag="IWK54_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9592629.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22035..22712
                     /gene="phoU"
                     /locus_tag="IWK54_000482"
     CDS             22035..22712
                     /gene="phoU"
                     /locus_tag="IWK54_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9592630.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            22950..23981
                     /locus_tag="IWK54_000483"
     CDS             22950..23981
                     /locus_tag="IWK54_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9592631.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            23978..24745
                     /locus_tag="IWK54_000484"
     CDS             23978..24745
                     /locus_tag="IWK54_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9592632.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24845..26413
                     /gene="liaX"
                     /locus_tag="IWK54_000485"
     CDS             24845..26413
                     /gene="liaX"
                     /locus_tag="IWK54_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9592633.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26713..27030
                     /locus_tag="IWK54_000486"
     CDS             26713..27030
                     /locus_tag="IWK54_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9592634.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27030..27380
                     /locus_tag="IWK54_000487"
     CDS             27030..27380
                     /locus_tag="IWK54_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9592635.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27547..28485
                     /locus_tag="IWK54_000488"
     CDS             27547..28485
                     /locus_tag="IWK54_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9592636.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28500..29330
                     /locus_tag="IWK54_000489"
     CDS             28500..29330
                     /locus_tag="IWK54_000489"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9592637.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29374..30399
                     /locus_tag="IWK54_000490"
     CDS             29374..30399
                     /locus_tag="IWK54_000490"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9592638.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30414..31352
                     /gene="galU"
                     /locus_tag="IWK54_000491"
     CDS             30414..31352
                     /gene="galU"
                     /locus_tag="IWK54_000491"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9592639.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31477..31917
                     /locus_tag="IWK54_000492"
     CDS             31477..31917
                     /locus_tag="IWK54_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9592640.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            31920..32522
                     /locus_tag="IWK54_000493"
     CDS             31920..32522
                     /locus_tag="IWK54_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9592641.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32581..33687)
                     /locus_tag="IWK54_000494"
     CDS             complement(32581..33687)
                     /locus_tag="IWK54_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9592642.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            33897..34898
                     /gene="ccpA"
                     /locus_tag="IWK54_000495"
     CDS             33897..34898
                     /gene="ccpA"
                     /locus_tag="IWK54_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9592643.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35235..37580)
                     /locus_tag="IWK54_000496"
     CDS             complement(35235..37580)
                     /locus_tag="IWK54_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9592644.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37859..38761
                     /locus_tag="IWK54_000497"
     CDS             37859..38761
                     /locus_tag="IWK54_000497"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9592645.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            38974..40749
                     /locus_tag="IWK54_000498"
     CDS             38974..40749
                     /locus_tag="IWK54_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9592646.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40815..42401)
                     /locus_tag="IWK54_000499"
     CDS             complement(40815..42401)
                     /locus_tag="IWK54_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9592647.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42652..42876
                     /locus_tag="IWK54_000500"
     CDS             42652..42876
                     /locus_tag="IWK54_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9592648.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43018..44769
                     /locus_tag="IWK54_000501"
     CDS             43018..44769
                     /locus_tag="IWK54_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9592649.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44769..46553
                     /locus_tag="IWK54_000502"
     CDS             44769..46553
                     /locus_tag="IWK54_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9592650.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46879..47730
                     /locus_tag="IWK54_000503"
     CDS             46879..47730
                     /locus_tag="IWK54_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9592651.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47784..49535
                     /locus_tag="IWK54_000504"
     CDS             47784..49535
                     /locus_tag="IWK54_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9592652.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49539..49709
                     /locus_tag="IWK54_000505"
     CDS             49539..49709
                     /locus_tag="IWK54_000505"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592653.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49749..50651)
                     /locus_tag="IWK54_000506"
     CDS             complement(49749..50651)
                     /locus_tag="IWK54_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9592654.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            50882..52285
                     /locus_tag="IWK54_000507"
     CDS             50882..52285
                     /locus_tag="IWK54_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9592655.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52285..53718
                     /gene="ascB"
                     /locus_tag="IWK54_000508"
     CDS             52285..53718
                     /gene="ascB"
                     /locus_tag="IWK54_000508"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9592656.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53863..54789
                     /gene="trxB"
                     /locus_tag="IWK54_000509"
     CDS             53863..54789
                     /gene="trxB"
                     /locus_tag="IWK54_000509"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9592657.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55003..56730
                     /locus_tag="IWK54_000510"
     CDS             55003..56730
                     /locus_tag="IWK54_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9592658.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56802..57095
                     /locus_tag="IWK54_000511"
     CDS             56802..57095
                     /locus_tag="IWK54_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9592659.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(57187..58047)
                     /locus_tag="IWK54_000512"
     CDS             complement(57187..58047)
                     /locus_tag="IWK54_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9592660.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58139..58567
                     /locus_tag="IWK54_000513"
     CDS             58139..58567
                     /locus_tag="IWK54_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9592661.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58729..58905
                     /locus_tag="IWK54_000514"
     CDS             58729..58905
                     /locus_tag="IWK54_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9592662.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            58932..59378
                     /locus_tag="IWK54_000515"
     CDS             58932..59378
                     /locus_tag="IWK54_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9592663.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59532..60911)
                     /gene="brnQ"
                     /locus_tag="IWK54_000516"
     CDS             complement(59532..60911)
                     /gene="brnQ"
                     /locus_tag="IWK54_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9592664.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            61617..62588
                     /locus_tag="IWK54_000517"
     CDS             61617..62588
                     /locus_tag="IWK54_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9592665.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62612..64804
                     /locus_tag="IWK54_000518"
     CDS             62612..64804
                     /locus_tag="IWK54_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9592666.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64821..65297
                     /gene="ybeY"
                     /locus_tag="IWK54_000519"
     CDS             64821..65297
                     /gene="ybeY"
                     /locus_tag="IWK54_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9592667.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65275..65676
                     /locus_tag="IWK54_000520"
     CDS             65275..65676
                     /locus_tag="IWK54_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9592668.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65691..66590
                     /locus_tag="IWK54_000521"
     CDS             65691..66590
                     /locus_tag="IWK54_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9592669.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66733..67545
                     /gene="recO"
                     /locus_tag="IWK54_000522"
     CDS             66733..67545
                     /gene="recO"
                     /locus_tag="IWK54_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9592670.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            67929..68846
                     /gene="glyQ"
                     /locus_tag="IWK54_000523"
     CDS             67929..68846
                     /gene="glyQ"
                     /locus_tag="IWK54_000523"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9592671.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            68848..70923
                     /locus_tag="IWK54_000524"
     CDS             68848..70923
                     /locus_tag="IWK54_000524"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9592672.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23222 a        12429 c        15179 g        20131 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaacgaaaa cttgcagcag ctactgacag ctgccgcaag tttttttgag tttcctcctc
    10141 ttttagatga aatatgaata aattatgaag ttcttacttt aatacgctta agatatttca
    10201 attatctata actaatgata aaatgaaaag taaagtctac atagtctgga taatacacag
    10261 aaggaaaagg agattacaca acgcatggcc aatttgattc gttcacttat cgaaaacgat
    10321 aagaaagaac taaaacgttt agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa
    10381 atggcagcat taactgatga acaattacag gcaaaaactg ctgaattcaa gggcagatac
    10441 caaaaaggag aaacattaga tcaattactt ccggaagctt ttgcagtcgt ccgagaagca
    10501 gcaaaacgtg tattggggtt atatccacac cacgtacaat tgatgggtgg aatcgtgctt
    10561 cacgatggaa atatccctga aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg
    10621 ccagtatatc taaatgctct gactggcgaa ggggtacacg tagttaccgt caatgaatat
    10681 ttagcaaccc gtgactcgac ggaaatgggc gaactttata atttcttagg attaacagtg
    10741 gggttgaata tcaactcaaa atcttcggat gaaaaacgcg aggcttataa ttgtgatatc
    10801 acttatagta cgaataatga attaggattt gactacttga gagacaatat ggtcgtttac
    10861 cgccatcaaa tggtacaacg tccgctgaat tatgccattg tcgatgaagt tgactcaatc
    10921 ttgattgatg aagcgagaac gccgctgatc atttcagggc aagcagaaaa atccacagct
    10981 ttatatacaa gagcagacaa cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc
    11041 gatgtacaat caaaaacaat cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca
    11101 ttcggtttgg ataacttata cgacattgaa aatactgcat tgacccatca cttggatcaa
    11161 gcattgagag ccaactatat catgctttta gatatcgatt atgttgtcca agacggaaaa
    11221 gtactgattg ttgaccagtt cactggtcgg atcatggatg gacgccgtta ttcagatgga
    11281 cttcaccaag caatcgaagc taaagaaggc gtagagatcg aagacgaaac gaaaaccatg
    11341 gcgacgatca ctttccagaa ctatttccgt atgtacaaga aacttgcggg aatgactggt
    11401 acagctaaaa cagaagaaga agaattcaga gaaatttata acatccaagt ataccagatt
    11461 ccgacaaatc gtccggttat ccgtgatgat cgtccagatt tgctttaccc aacattacaa
    11521 agcaagttcc acgctgtcgt gcaagatatc aaagatcgct accgcaaagg tcaacctgtg
    11581 ttagtcggaa ctgttgcggt tgaaacatct gagttattat cagatatgct gaatcaagaa
    11641 cgtattcctc atgaagtgct gaatgcgaaa aatcacttta aagaagcaga gatcatcatg
    11701 aatgctggtc aaaaaggtgc tgtgacgatt gctaccaaca tggctggtcg gggaacagat
    11761 atcaagttag gacttggggt aagagaactt ggtggtctag cagttatcgg gacagaacga
    11821 catgaatctc gtcgtatcga taaccagctt cgaggacgtg caggacgtca aggagaccct
    11881 ggtatgtcac agttctactt gtcattggaa gatgatttga tgaaacgatt cggttcagaa
    11941 cgaatcaaat ctttcttaga tcggatgaag atcggtgagg aagatgctgt tatccaaagc
    12001 aagatgctga cgaaacaagt agaatctgca caaaaacgtg tagaaggaaa taactatgac
    12061 acacgtaaaa acgtcttaca atacgatgat gtgatgcgtg aacaacgtga agtaatctat
    12121 aaacagcgac aagaagtaat catggaagaa aaaagcttat ccaagccact aatgaacatg
    12181 gtcaaacgga caatcagtcg tgtagtagat gcgcatacac aattagagaa agaaaattgg
    12241 aatttggaaa gtatcatcga ttttgccggc agtactcttg tccatgaaga tagtattact
    12301 cttgcagata tcgaaggaaa aacagctcag gagatcaaag agtatctaaa cgaacgagca
    12361 caagaagtat ttaataccaa agcggctcaa ctaaatggtc cagagcaact gttggaattt
    12421 gaaaaagtcg tgatcttgcg tgttgtggac tctaaatgga cagaccatat tgatgcaatg
    12481 gatcaattac gtcagtcaat cggcttacgt gcttatggac agaacaatcc attagtcgaa
    12541 tatcaaacag aaggctataa gatgtttgaa gatatggtag gcgccatcga gtatgaagtc
    12601 actcgaatct ttatgaaagc tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa
    12661 ggaacagctt ctcactcaga agacggagat gccaaagaaa atagtaatac tagcgcgaaa
    12721 aaacagccaa tccatgtgaa caaggtcgga agaaatgatc cttgtccttg tggaagcggg
    12781 aaaaaatata agaactgtca tggaaaaaat gcaaactaat ttctgacagc aggattggtg
    12841 agacaaattc tttttttaag aattcgtctc accttctttc tgttatttgc tgattctgtt
    12901 agactagata taatgaaaaa agtatagtga aagaaggaaa aaacatggaa atatccgaaa
    12961 ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag cttcaggagg tctctttgac
    13021 ttagaccagc tggaagaaga cattgcggaa gcagaagcaa aaatggctga acctggtttt
    13081 tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa acaatgcaaa taaagaaact
    13141 tatgatcaat tccattctct tgctgaagaa ctggaagaaa tcgaattgat ggtggagatg
    13201 ttgcaggaag aacctgatgc agccatgcaa gaagaagcag aaacacgtat caatgcgcta
    13261 gatgaaaaaa tgaagacata tgaattatct atgttgttag atggtcctta tgatcgaaac
    13321 aacgccatcg ttgaattgca tccaggggct ggcggaacgg agtcccaaga ttggggcagt
    13381 atgcttttac gtatgtatac acgctgggca gagcagcatg ggtatcaagt agagacacta
    13441 gattatcaag caggtgatga agcaggaatc aagagtgtga ccctactgat caaaggacac
    13501 aacgcatttg gttatctgaa atcagaaaaa ggtgttcatc gccttgtacg tatctcgcct
    13561 tttgactcag cgaaacgacg tcatacttct ttttgttcgg tagatgtgat gccagagctt
    13621 gatgaaacga tcgatataga aatcaatcca gatgatttga aaatcgatac gtaccgagca
    13681 agtggtgcag gtggtcagca catcaataaa acagaatctg ctgtacggat cactcacttg
    13741 cctacaggaa cagtagtagc tagtcaggca caacgttcac aattgaaaaa ccgtgagcaa
    13801 gcgatgggga tgttaaaagc taagctttat caattagaag tggaaaagaa agaacaagaa
    13861 gcagctgcac ttcgaggaga acaaaaagaa atcggctggg gttcacagat ccgttcttat
    13921 gttttccatc catattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc
    13981 caagcagtta tggacggtga ccttgattta tttattgacg gctatttaaa aatgcatcta
    14041 caataagaac ccattgttaa attgaaataa aattgtaaga ttttgcaagc atgttgaaac
    14101 attgccatgc tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa
    14161 tgaaggatgt aatgaagaag tactcaaatg gtacgaccgc tatccgcaat ctctcaattg
    14221 tcatcgaaca aggagagttt gtctatgtgg ttggtccgtc tggagccggt aaatctacat
    14281 tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc
    14341 atgatctaat agcaatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg
    14401 tgttccaaga ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta
    14461 tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg agtaatggaa gtgctggacc
    14521 ttgttggttt gaaacacaaa gttcgagtct ttcctagcga attatccggt ggggaacagc
    14581 aacgtgtctc tattgctcgg gcaatagtga atacgcctaa agtacttatc gcggatgaac
    14641 cgacaggaaa tctggatcca gaaaactcat gggaaatcat gaaattatta gatcggatca
    14701 atgcccaagg aactactgtc gtgatggcta cacataacag tacgatcgta aatacgattc
    14761 ggcaccgagt aattgctatc gaaaacggcc gcattattcg agatcaagcg gaaggggaat
    14821 atggatacga tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa
    14881 cgtaacggct ggatgacagt tgcttcagtc agtgcggtaa cgatcacatt gactttagtc
    14941 ggtgtattca tggcagttat catgaacgca actaaattag ctcaagatat agaaggaaac
    15001 gttgatgtct ctgtattcgt agatatcggt acaaaagacg aagagatgaa aaagctagaa
    15061 aaagaattga atgacttaga tcatgttaaa aaagttgaat tttcaagcaa agaacaggaa
    15121 ttgaaaaaaa tccaaaacga aatgggtgat tcctggaaat tgtttgaagg tgacaataac
    15181 ccgttatatg atgtgtatgt agtaagtgct actgatcctt cttatacaaa acaagtagca
    15241 gaagaagcag cagatttgaa gaatgtttct cgttctgatt acggtggagc ttcctcagat
    15301 cggattttcc aaatcgctgg tgcagttcgg acatggggat tagttgcagc ggcattgctg
    15361 ctgttcgtag cgatgtttct catttcgaac acgattcgga tcacgatttt gtctagacag
    15421 cgtgaaattc aaatcatgcg tttagtcggt gcaaaaaatg gatatattcg ctggccgttt
    15481 ttccttgaag gtggctggat cggtctgatc ggtgcgattc ttccgatttt gatcatcaca
    15541 tttggttatg catggttttt ccaattgatc aatccatcat tattacgttc ccattattca
    15601 ttgattcatc cacaaaatat cgtttggaaa atcaatcttt taatggtcgg cattggtgtg
    15661 atcattggtt cacttggctc gatcatttct atgagacgtt tcttgaaatt ctaataccac
    15721 aatataaata aaatgagaaa gttggacagt ttcgttccag ctttcttttt ttattggcta
    15781 ggagaataga aggaagcggc ttttttccct ttactttaaa aaacaacttg ggtattctta
    15841 agttggtaga agaagaggtg aagccgtgaa aattatcgtt gacaattttt ttacgatcct
    15901 tttgattatg aatattttgc tttcctttat catcgttttt cgagaaagaa aggaaactgc
    15961 tcaaacatgg gcatgggtgc tcgttttgat gttcattcca gttgtaggat ttattttata
    16021 tattttttta ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa
    16081 aatcggtaag aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc
    16141 tcatccgcct actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga
    16201 aagtacgctc tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt
    16261 cgatgcgctg attgaagata tcaagcaggc agaagaccat atccacttcc agtattatat
    16321 ttatcgcagt gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg
    16381 aggggtaaaa gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt
    16441 ttttcagaat ttgaaaaaag caggcggaga agtcgcattt ttctttcctc tatttgttcc
    16501 gtatattaat cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa
    16561 aatcggatat acaggtgggt tcaatgttgg aaatgaatat ctaggattgg taaagaaatt
    16621 tggttattgg cgagacaatc atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa
    16681 ccgttttttg atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt
    16741 ttttccgtcc attcactcta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga
    16801 taccgaacat gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga
    16861 gatattgatc caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt
    16921 ggctcttctt tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct
    16981 cgtttactgg gcaacgtatt ctttcgctgc agagctaatc gagtatggag caaggatcga
    17041 aacatatgag aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc
    17101 agttggctca gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact
    17161 ggtttatgat gaacgaatgg cttcacgtgt aagaaaagct ttttttgaag actcgaaaat
    17221 ttctacgcat ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga
    17281 aggattagca agattgattt cgccgctcct ttaaaacata aaaaggacat gagaaccatc
    17341 aacagatgga gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat
    17401 ggagaaagcg tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa
    17461 gcaagaaata agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata
    17521 gaaactttta atgagatagt tttttttaca attatgagaa tttattttgt tatttaatga
    17581 ttttgattcg ttttcatcaa gtaatgaaaa tgataaaata aacatacttt aggattaaag
    17641 gagatttcta atgaaaaaac ttaaattatt cttaccaata ataggattag ctattctttt
    17701 atcaggctgt tcaaagtgga ttgatcgagg agaatccatt acggctgttg gctcttctgc
    17761 tttacagcct ttagtagaaa cagttgctga aacttaccag acagaaaatc ctggaaaatt
    17821 catcaatgta caagggggcg gaagcggaac tggtttgtct caagtacagt caggagcagt
    17881 cgacatcgga aactctgatt tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt
    17941 agtcgatcat cgagtagcag ttgttgggat cactcctatt gtgaacaagg atattggcgt
    18001 aaaagacatc agtatggaaa acctgaaaaa actatttaca ggcgagatca aaaactggag
    18061 tgaagtcggt ggaaaagatc agccagtagt tatattgaac cgcgcttcag gaagcggaac
    18121 ccgtagcacg tttgaaaaat gggtattgga cggagaaaca gctgttcagg cacaagaaca
    18181 agattcaagc ggaatggtgc gccagatcgt tgccgacaca cccggtgcaa tcagctatgt
    18241 tgctttttct tatgtttccg atgatgtaga tacgttgaat gttaacggtg tagaaccgac
    18301 agatgaaaat gtgacaacga acaaatggac gatttgggca tatgagcata tgtacacaag
    18361 agatcatcca aaaaaactga caaaagaatt tttagactat atcttatctg atgaagtaca
    18421 agataacatc gttggagaac taggttatat ccctgtttct catatgaaag tcgaacgtga
    18481 ttggcaagga aatatcgtta aagagtaatc tttacacaaa atttacaaac ttagctaaac
    18541 ttttacatgt tattaaaacc aaattgatgt tcaatttgta cactgagtga gtatgttaaa
    18601 cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa
    18661 acgttcaaaa atggaacagc gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt
    18721 gttggttgta ttatctattt tttattttgt agctagtaaa ggattggcca ccttttttac
    18781 tgataagatc aatgtatttg attttctgtt tggtacagat tggaatccaa gccaaatcgg
    18841 agcaaatggg aaaccaatgg taggggcatt gcctatgatc acaggttcgt ttattgtgac
    18901 ctttttatct gctatcgttg ccacgccatt tgccattggt gcagcagtat tcatgacgga
    18961 gatctctcct aaaaacggaa cgaagatctt acagccggtc atcgagctgc ttgtaggtat
    19021 tccttctgtc gtttatggtt ttatcggttt atcagttatc gtgccttttg tcagaagtat
    19081 ttttggcggg actgggtttg gtatcttagc tggtacattt gtgttgttcg ttatgatctt
    19141 gccgactgtc acaacgatga ctgttgatgc attaaaggca gtgccacggc actatcggga
    19201 agcttcatta gcgcttggtg ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc
    19261 agccgtgccc gggattttaa cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc
    19321 cttagctatc caaatggtca tcggtaatgc agcattgatg ccaacaggtc ttactactcc
    19381 tgcttctaca ttgacgtcca tcttaacaat gggaatcgga aatacaatca tgggaacagt
    19441 tgaaaataat gtgttatggt cattagcgct gatcttgcta ttgatgtcat tattctttaa
    19501 tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa taatgaacgc aaaacgctca
    19561 gataaaattg ctacaggtgt tttatatata gtttcgggga tcatcgtatt gattttagca
    19621 gctttactac tttatatctt agttcgggga ataccgcaca tttcatggtc cttcttgacc
    19681 gaaccttcca aagcctacca agaaggcggt ggaatcggga tccagctatt taactcgatt
    19741 tatttgcttt tgatcacgat gatcatcagt tttcctattt ctttaggtgc gggtatctat
    19801 ctttcagaat atgctggaaa aaactggcta acagatgtga ttcgcacgtc gattgaaatc
    19861 ttgagctcgt tgccttctgt cgttgtcggt ttattcggtt tcttggtttt cgttattcaa
    19921 ttccagtatg gtttctcgat tatttctggt gcattggctt tgactgtatt caacttgcct
    19981 cttttgacta gaacagttga ggagtcttta caagcggtcc attacacaca acgagaagca
    20041 ggattggcct taggtttgtc acgctgggaa acagtgataa aagtggtggt tccagaagcg
    20101 acaccaggga ttttgactgg tgtgatccta agttctggaa gaatttttgg tgaagcagct
    20161 gcattgatct atacggctgg acaaagtgca cctgcgttag attttacgaa ctggaatcca
    20221 ttaagtgttt caagcccgat cagcattttc cgtcaagctg aaacattagc tgtccatatt
    20281 tggaagatca atacagaagg cacgatgcct gatggtgttt ctgtttcggc tggcgcttct
    20341 gccgtgttga ttattgctgt tttactgttc aacttcggcg ctcgtgcgat tggcaaacga
    20401 ttataccgaa aaatgacttc agcctaagga gaagaagcaa aatgaaaaaa tataactgga
    20461 atgaaacaaa tgtcatcacc atgccaaaag agaaagaagt agcactatat acggatgatc
    20521 tacatgtctg gtatggcgac aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa
    20581 ataagatcac tgcactgatt ggtccttccg gctgcggaaa atctacgtat cttcgttcgt
    20641 tgaatcgaat gaatgatggg atcgctaata cgaaaatcac tgggaaaatc atgtatcaag
    20701 gcgtagatat taatgcgcct caagtggatg tttatgagat gcgcaaacgg attggcatgg
    20761 tttttcaacg tcctaatccg ttcagcaagt cgatttatga caatattaca tttgctttga
    20821 aacagcacgg cgaaaaaaac aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac
    20881 aagctgcctt gtgggaccaa gtaaaagaca gcttggacaa aagtgccttg gctttatctg
    20941 gcgggcaaca gcaacgttta tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc
    21001 tgctagacga gccggcaagt gctttggacc ctatttcgac aggaacggtt gaagaaacac
    21061 ttgtaaactt gaaagaagac tatacgatca tcatcgtgac gcataacatg caacaagcag
    21121 ctcgtatcag cgactatact gcctttttct atttaggaaa agtaatggaa tatgacaaaa
    21181 ctaacaagat atttaccaga ccaaaaattc aggcaaccga agattatgtg tccggtcatt
    21241 ttggttaaga cggaggcctc aacatgacaa aagagattat ttcatctaaa gatcttcatc
    21301 tgtattatgg aaagaaagaa gcactaaaag ggatcgatct aagcttcaat caaggggaaa
    21361 tcactgcgat gatcggtcct tctggttgcg gaaaatccac ttatctgcgt tctttgaacc
    21421 ggatgaatga cctgattccg aatgtaacga tcaccggaag tgttttatat aaagacaaag
    21481 acatctatgg tccaaaaaca gatgttgttg aactaagaaa agaaatcggc atggtattcc
    21541 agcagccaaa cccatttcct ttttctatct atgaaaatgt catctatgga ttgaaattaa
    21601 aaggggaaaa agataaaaaa gtccttgatc aagtagttga agaaagcctg aaggcagcgt
    21661 ctgtttggga tgatgtgaaa gacaaattgc ataaaagtgc tttatcttta tccggtgggc
    21721 agcagcagcg tgtatgtatc gcacgtgttt tggcagttaa tccggaaatc atcttattgg
    21781 atgaaccaac aagtgcgctt gatcctgttt ctactgggaa aatcgaaagt atgctgttag
    21841 aattaagaga gcggtatacg atgatcatcg tgacccataa tatgtctcaa gcttcacgta
    21901 tttcagataa gacggcattc tttttagatg gtcacttgat cgaatacaat gatacgaaga
    21961 agatattcat gaatccagaa aaacaagaga cagaagatta tatttcaggt cgatttggat
    22021 aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga attgctgaat ctccataatc
    22081 agttctacga aatggggatg atggtgagca gcgccgtgca caagtcagtt cgtgcataca
    22141 tcaaacacga taaaatctta gcccaagaag ttatcgaaaa cgatataaat atcaataaca
    22201 tggaaacacg cttggagaag aaaagttttg aaatgatcgc attgcaacag ccagttacaa
    22261 ctgatttgcg tatgatcatt acggtcatga aagctagctc ggatttagaa cgtatggccg
    22321 atcatgcggt gtctgttgct aaatccacga ttcgcgtaaa agggcagacg agaataccgg
    22381 aaatcgaaaa agagatctcc gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc
    22441 tagttgctta tgtcaaaaca gacgaaaaag acgcacgtac gatcgccaat atggatcaaa
    22501 gagtcaatga ctatttcaat cgaatctata atgcaacaat caaaaatatg caggaaaatc
    22561 ctgagacagt catcagtgga acagactact tgaatgttgc cagttatcta gaacggatcg
    22621 gagactatgt gacaaacatc tgtgaatgga tcgtctactt ggctacaggg aaaatcactg
    22681 agttaaatac gaaccacaat gagattttct aaactgaagc tgtgattatg ccgtttgttt
    22741 aggaaaagag gttgtgaaat cagcggttgt gaaaaagctg ttctgcatca agtaataaga
    22801 acagtcaaac aaaaaatagc tcctcatatt tttcgtttgg ctggacttat taccgcagat
    22861 gcaagctttt gaacaccgtt tattcgggaa agggtttgtg acaaaacatg tcacaaaccc
    22921 ttttcatcat ttcatagaaa ggataaagaa tgcaaaaaaa aattagcatc atcactatat
    22981 tttttatttg tgtgcttact tcatgggtta ttcagcatca agccgcttta tatgaagtac
    23041 ctgtcatcga gatagaaaaa gtacgaataa aagaggatca tcaattgatt acaggaaaac
    23101 tgatgaacac ggatcaaaaa ggagaatcga tttatttatc ggttccttat caaaaaaatc
    23161 aaatagaaac agcaaatata aaagcgagac aaatgtatct agtccaaatg catggagatg
    23221 aatggcagct agtagcaaaa aaaaatgatg gctggctttt cttcttcaca agtatcttca
    23281 ttggcgccat gttgcttatt ggtggaaaac agggtctatt tgctttagga agtctcatca
    23341 tcaatatcgt tttactttta cttttgttgc tcatctttac taaaacagaa gggatttctc
    23401 tattaaatat atctatcctg ttcgtcgtta tagggatgat tattagcata ttggctttag
    23461 atggatggaa tagaagcagt ataatcaaaa tttcagctgc agttacgagt gtttttttag
    23521 cattttttat ttgtcttttg acaatgaatc attggaaaga ccaaggactg cgctatgagg
    23581 aactgaatta tctgacacga ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa
    23641 cagctggagg aacactcgat accgttatca cagtcattgc gacattagaa gaattgacta
    23701 agcgacaacc agaaataaca gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag
    23761 atgtgattgg ctcgatgacc aatatccttt tgttcgtcta tatcagtggg tcagttccat
    23821 cgcttctttt atatttagga aatcaatggt cgttcaaaga aacgatagaa cagcatttat
    23881 cattggaatt tttacgcgtc attgctggca gcttaggaat agtattatcg atccctatct
    23941 cggtcctctt ttttctgact gtaaggaggc tgaaacgatg aatgtactta ttatacttgt
    24001 tgggatttta gcaatcagcg tgctacttat tggcggtacg caaggtatgt atattctttt
    24061 aggcttattc atcaatttag ggatcttttt cctgttgtta ttcggttatc atcagaagtg
    24121 gccgatactg gcattgtcgg tcataggatt tttattgatt gctgtggtta tcttattttt
    24181 tatcaacggt tataatctaa aaatgcgtgc agcttttgtg agtatactga tctttttatt
    24241 ttgtttccta ctattgcttc ctatcactga ttttttagca atccaaggtt ttacatcggt
    24301 agaactagaa gaattatctg gcttagataa gactttagcg attgatttta gattacttac
    24361 gagatcgttg cttctgatta gtcttagtgg tgctgtttta gatgcgagtg tagcaatttc
    24421 tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta tctttttacc aattgcgcca
    24481 ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc
    24541 attcatggga agttctttag cgttgatcct ctggtttatt gatttagaca ttccttttca
    24601 gcaagtgatc aatgaaaaat catttgttct agaatatacg atggctattc ttaccacatt
    24661 gtccgcacta cttgttcttc ctttaacctg cgtgactaca gcatatctct ttacaaaaaa
    24721 gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag tcctatgaca tttttactga
    24781 atcagtgcat aatatggatg taaggaaaag cttgcaaaca attttgctta aggaggcaat
    24841 ttccatgaaa gaaagagaac gtattttaga tttagtgaaa aaaggaatcc tttcgactga
    24901 agaaggctta gatttattag aaagcatggc aactgaaaaa gacgaaaagc aaatcaaaaa
    24961 agaagcagac aaggtaactg ctagtcataa agaaaaagat caggcaagtc aattgatcga
    25021 taaacttgaa aacggcgaag aagaaatttc tgagccagtt gatccaaagg aaagagaaag
    25081 acaagatcaa gaaaacttag aaaagatctt agatgaacta gcaactgaag cgaataaaac
    25141 ttcggcgcgt ctagatgaag taaatgctca attcgctgac aataaggcag cacgcaatga
    25201 gaaacaggaa gcattgatgc agctgaatac aaaagaagaa ttaggcgagt tgacagaaga
    25261 agaattagca cagcgtcaga cgttagaagc agaaatcaaa gaattagaag ctagtggcga
    25321 tacactgtta gaagaacaat tgaaattaga agcagaatta aaagatatcc gtaagaatca
    25381 atggtctgag aaaaaagaaa cgtttactga taagtttgaa ttgccagatg attggaaaga
    25441 tcaagcaaca gatacactga atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt
    25501 aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa acagtgaatg acaacatgga
    25561 atggaaagat gtcagcttac gtgtccctgg gattgcgaca acgaaatttg agcatgaatt
    25621 ttactatgaa gctcctgcag caagtatcct tgatatcaag gcagcaaacg gaaacgtgac
    25681 gttaaaaacg tgggacagca acgatgtgaa agttgaagct aagatcaaac tttacggaaa
    25741 aatgggtgct gaaccatttg aagcattctc tgaacggagc cagattgagg ttaatgaaga
    25801 tcatatctcc ttccaaattc caaataaacg tgtgcgtgca gaccttgttt tctacttacc
    25861 taaacgagta tacgaccacg cagctattaa acttctaaat ggcaacatca tgattgaaac
    25921 attggaagct aaagatattt atacaaaatc tacgaatggt aacatcatcg tggaccaatt
    25981 aactgcaacg atgctggaag tagaaggtgt aaacggcaat atcgatattc gaaacgggaa
    26041 catcttagat tctattatcg aaacagtaaa tggtacagtg acatttggtg cgacagcaga
    26101 aaacctttct gtgtctttgg taaatggtga tgtacgtctt acgattaaag aagataattt
    26161 gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat
    26221 cggtttggaa ggacatgcta aaacaagtct tggaagtatc aacagccgtc tgtccaatta
    26281 cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc
    26341 agatggtgaa atcgcgcaag tgcaactttc aacgacaaca ggaagtatct atttaaaaga
    26401 tacagataaa taaagaggtt gtgaaagaga tgttcagcct tgagcaataa gaagcgattt
    26461 tgtaaaatag cttcccctat tttacaaaat tgtgccttat tgccgatagt ctatctcttg
    26521 aacaccgttt accaagagat tatgaacctg ccaattatcg ccgagtcaca aggaataatt
    26581 tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg tgaccgagaa ttttgcagaa
    26641 agacaccgat taagaagaag gttgtcacaa cgattttctt catttaaaat agtagaacag
    26701 aggtgagaca tgatgaaaaa aaagttgaca aaatctaata aaaatgttgt tttgacaggg
    26761 actttagcag gaataggaga atacatcggg attgatccga ctgttatccg tgtgctttat
    26821 gtattcctga gcttagttgc ttttggaagt ccaattattt tatacatttt attagctttg
    26881 attatcccat ctgcgcgttc tgcacgaaca aactatggac ataacaatgc atattatgaa
    26941 aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa
    27001 atcgatgatg acgaatggag tgacttttaa tgtcgtactt tcaacgtatt ttagttaata
    27061 cgttaacatt catatcattg gctgttcttt ttcctgcgat ggtccatgtg agcagtattt
    27121 ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt
    27181 tgacgatact gtccctgccc tttacattag tcacattggg attgtttacg tttgttatca
    27241 atgcagcgat tcttaaaatg acctcctttt ttgtgggagc agaacatttt agtttttctt
    27301 cattcggtgc agcgttgtta gtagctatct tgatgtcgct tgtcaatgta attgtcagcg
    27361 aacataacag tagaaaataa aagaaagctg tgccgaacta tcggcgcagt ttttttattt
    27421 agaatcagga atgcgttttc tattacattg gtaattaaat aaaaacgttt tttttaattg
    27481 gtagtacttt tggctagaca atgataaaat gatagagaac actctaatag aaatgaggta
    27541 gcatgaatgt cagaagttgt aaaagttagc caattagtga aaaaattatc tttggaaatc
    27601 gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa ctggggacat ctcacgccca
    27661 ggactagagt tgactggtta ttttaattat tattcccata atcgtttaca gctatttggc
    27721 agtaaagaaa tcagttttgc ggaacggatg atcccagaag agcgtttgat catcatgaga
    27781 agattatgta gtgaagatat gcccgcattt attatttcaa gaggattacc agctccagaa
    27841 gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac tgcgttctcc catctcaact
    27901 tctcgtttat taggagaatt atccagttat ttagatggtc gccttgcccc tcgaacaagt
    27961 gtccacggtg ttttagtgga tgtttacgga ttaggtgtac tgattcaagg tgacagtggg
    28021 ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg gccatcgatt gatcgcagac
    28081 gatcgagtag atgtttatca gcaagatgaa ttaacggtga tcggagaacc gcctaaaatc
    28141 ctggaacatc tgattgaaat ccgaggaatc gggatcattg atgtaatgaa tctttttggt
    28201 gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg tttatctgga agcatgggca
    28261 aaagataaga aatacgaccg tctgggctct gaagacacga ccgttgaaat cgcggaagtg
    28321 ggcattccac aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat catcattgaa
    28381 gtagccgcga tgaatttccg cgcacgtaca atgggctttg acgcaacgaa aacatttgaa
    28441 gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg actagcaagg agtgacaata
    28501 tgttagcaca aatcaatcga gttgcttttg agcttgtagg gattcctatc tactggtatg
    28561 cattgatcat cgtatcaggt atcattatcg ctatgtggct tagcagcaga gaggctgttc
    28621 gggtaggatt aaaagcagat gatgtgacag attttatgtt gtggggattg cctatctcca
    28681 ttattggcgc tcgcttatat tatgttttat ttgatcttcc ccagtatata gctgatccaa
    28741 tccaaatatt caatatccgt tcaggaggct tagctattta tggcggactg attgcaggcg
    28801 cgatcacgct gtattttttt actaaatatc attttatttc tttgtggact tttttagata
    28861 tcgctgcacc aagtgtatta ttagcacagg caatcggtcg ttggggaaat ttcatgaacc
    28921 acgaagcata tggaccagac acgacgcgaa gcttcttaga aagtctgcat ttaccgcaat
    28981 tcatcattga taatatgtat atcgaaggga tctatcgtca accaacattt ctttatgaat
    29041 cactttggag tttgattgga tttattttgc ttttgttttt acgaaagaaa ccacaccttt
    29101 tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta tagtttcggg cgtttcttta
    29161 ttgaaggatt acgtacagac agtctttact ttttaggtgt gatccgtatt tctcaattat
    29221 tatctgctgt gttatttgtc ggtacattga ttctatttat ttggcgcaga aaaaaaggaa
    29281 atttaccgga ttatgaccgc tcattaggaa aaaatcaaca aattatctga tttttagagc
    29341 gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt atagagtagc ggttttagga
    29401 ccaggctctt ggggtactgc tttggcacaa gtcctcgccg aaaacggaca tgacgttcgg
    29461 atttggggac atcgtgcttc acaagtagaa gaaatcaaca cccaacacac gaatcaacgt
    29521 tatttaccag atcattatct acccgattcc atccaagcat tcaccaatat ggaagatgca
    29581 gtaaaagacg cagatgcggt gctattcgtc attccaacga aagcaatacg ttctgttgca
    29641 aaagaactag tgcctaaatt gaatacaaag ccagtgatca tccatgcaag taaaggatta
    29701 gaacagggaa cacataaaag gatctctgac atcttgttag aggaaatccc agcagaaaaa
    29761 agaaaagctg ttgttgttct ttctggacca agccatgcag aagaagtagc aaaacatgac
    29821 atcactacga tcacagctgc aagtacagat gcaaaagcag caaaatatgt acaagagctg
    29881 ttcatgaata attattttcg gatctatacc aatcctgatg tgatcggtgt agagatgggg
    29941 gcagctttga aaaatattat tgctattggt gctggcgcta ttcacggctt agggtttggc
    30001 gataatgcaa aggcagcaat catgacgcgc ggattagctg agatcagccg tttaggtgta
    30061 gcaatgggtg ccaacccgct gacctttatc ggattaagcg gaataggcga tctagtcgtt
    30121 acttgcacaa gtgttcattc taggaactgg cgcgcaggca acttgttagg acaagggcag
    30181 cccctagaag aggtgctagc caatatggga atggtcgttg aaggtgtgaa tactacccaa
    30241 gcagcgatgg aactagccca aatgatggaa atcgaaatgc ccatcactca aacaatctat
    30301 gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat gttacgtgat
    30361 ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa ggagcgaatg tatatgaaag
    30421 ttaaaaaagc agttattcca gcagcaggat taggaacaag atttttacca gcaacaaaag
    30481 ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac aatccagttc atcgttgaag
    30541 aagcgttgaa atcaggtatc gaagacatct tgatcgttac aggaaaagct aaacgaccaa
    30601 tcgaagatca ctttgattca aatattgaat tagaaacaaa cctaagtgaa aaaggaaaga
    30661 ccgaattatt gaaattagta gaagaaacga cagatgtcaa cttgcatttc atccgtcaat
    30721 ctcatccaaa gggtttagga catgccgttc ttcaagcaag agcctttatt ggaaatgaac
    30781 catttgtcgt tatgctaggc gatgatataa tggaagacga agtaccattg acacagcaat
    30841 taatcgatga ttacgaacgt actcatgctt ctacgatcgc cgttatgcaa gttcctcatg
    30901 atgaaacttc aaaatacgga attatcaatc caggagaagt attggaagat gggttataca
    30961 atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc accaagtgat ttagctatta
    31021 ttggacgtta cttattgact ccagaaattt ttgatgtatt gtccgagcaa caaccaggag
    31081 ccggaaatga aatccaacta actgacgcaa tcgatacatt gaataaaaca caacgcgtat
    31141 ttgcccgtaa attcacaggt aaacgttacg atgtcggtga caaatttggt tttatgaaaa
    31201 caagtattga atatggtcta acacatccag aagtaggtgc acctttacgt gagtacatcc
    31261 tgtctttagg taaaacatta gaagcagaag gaagcaagat cgatcataat ccaaaaggaa
    31321 taacagaaat caaagaagaa aagaaaaaat aacccttttt tctaacgagc ttcagctgct
    31381 aattgaataa aaagtagttg aagctcgttt ttatttgttt aaaaaacacg aaaatgctat
    31441 ccttatagaa aagagagaca agaagggagt ttgatcatgt caggaggaga aattgcagga
    31501 ttgatcgcag caatcgcctt tgcgatactt gtcgttttta tcgttcgtgt gttgttgcaa
    31561 gtttctaaaa ctttagaaaa agtagatcag actgtagcag aagcaaatac gacgatcgaa
    31621 atcgtcacaa aagacgtaga tcttttgtct agacaagtag aaggcttact ggtcaaaagt
    31681 aatgaactgc tcttggatat caatggaaaa gtcgcaacga tcgatccatt gttcacagca
    31741 gttgctgatt taagtgaaag tgtatcagaa cttaatcatt ctagtaaaaa catcgcgacg
    31801 aaagtcggcg gaatcggtaa aacgacagct aaagcgacgg ttgccggaaa agtcggtggg
    31861 acagcgatga aattttttaa aaacaaaaaa catactgaaa cgacaggagg aaaataaaaa
    31921 tggctaaaaa aggtggattt ttattaggtg cagtagtcgg aggcacagcc gcagcaatcg
    31981 ccgcgttatt gttagcacca aaatctggta aagaacttcg ggatgaatta gctgcccaag
    32041 cggatgattt gatggatatg gcttcagatt acacagatat ggcgaaagaa aaatcgactg
    32101 agttaacagg tattgcaaaa gaaaaagcgc aagatctttc tcaacaagca ggtgatctag
    32161 caggtagtgt caaacaaaaa gtaagtgaat cagcagatga agcaggaagt gaatctgacg
    32221 atatcttgtt aaatcttaaa gaacaatcag ctgacttatc taatcgtttc aaaaaaacgg
    32281 cagaagatat gaaaaaacct gcagatgaat taggaagtat tgctaaagat acttcagaag
    32341 atatcatgat tgatgtaaaa gacgctgctg gtaaagcagg agatactgtc aatgaaggaa
    32401 tgtcagaagc aaaaccagtt gcagatgact caaaagaaat gatgaaaatg gcaaaagaag
    32461 atgcgaaaga agctaaaaaa gaaataaaag atacaatgac agattcatct acaaatcact
    32521 aaaatcacaa atttatagaa taaaaaggtg ggagcggact agctctcacc ttttttactc
    32581 ttatttttct aaaacaagta attcttttgg tgtagtcgtg aatggcacac agccatcgct
    32641 tgtcacatat acacagtctt cgatacgtac ccctactttt tcaggaatat agattcctgg
    32701 ttcaagagag aagcacatcc cttcttcgat tactaagtca tttccttcta caagggaagg
    32761 gaattcgtgg acggttgttc cgatcccgtg acctaaacgg tgattgaaat attcgccgta
    32821 gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg
    32881 acggacggct tcttgtgcgg caagctgtgc ttcaagagtg atgttgtaga ttttttcttg
    32941 gaagtcggtt ggtttttgat aagcaattgt acgagtagca tcactgcaat agccattcca
    33001 aacaacgcca agatcgaata acacaagttc attcggtgaa atcttcgtat ttcctggtgt
    33061 accatgaggg cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga
    33121 aacaccttgt tttttcaatt ggtattcaat ttctgcaatg atcgcttgtt cagtcacgcc
    33181 ttctttcact gctttaaagc caatctcaaa tgcaacatcg gcccatttac cagcttcgat
    33241 cagtttgtct ttttcctcta gagttttgat cagttgtaga cgttggatga tggaagttag
    33301 atcagtgtaa gaatccgttc ccgtcaagat atcagagata gcatcgaagc gctcgacagt
    33361 caaggcgttc ttttcaatcc ctacattgcc aggctgacca tagcgagcac gaagttcttt
    33421 tccgatgatc tcccaagggt tttcgctgtc taaatagcca cggacatcat acgcccaact
    33481 actgttttga gcatcttcaa cttccaaagc aggagtgaaa aggaaaggat cttgttcaag
    33541 agggataaaa agagctagta cacgctcatg aggatcactt tcgtatccgg aaaagtaagc
    33601 gatgtggcca gggtcgctga tataagccaa ttcgacttgg tgttgtgcca tccattgacg
    33661 tagttcattg atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt
    33721 caataattgt accactgatt tattaaaaga ccaacgttct aaaaactgaa aaaagtaaaa
    33781 attttcatga aaacagagat aaaacggttg caattcttga gattatttga tattctattt
    33841 tagaaatgta agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg
    33901 aaaagcaaac aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg
    33961 tttcacgtgt cgtaaatggc aatccaaacg ttaaaccagc aacaagaaaa aaagtcttgg
    34021 aagttatcga tcgcttggat tatcgtccaa acgcagttgc gcgtggcttg gcaagtaaga
    34081 aaacaacaac agttggtgta atcatccctg atgtcagcaa tatgttcttt gcttctttag
    34141 cacgtggtat cgatgatgtg gcaacaatgt acaaatacaa tattatctta gcaaattcag
    34201 atggcaacga ccaaaaagaa gtcaatgtat tgaataactt actggctaag caagtggacg
    34261 gcgtgatctt catgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa
    34321 aaactcctgt tgtattagct ggctctattg atccagatga acaagtaggt agcgtaaaca
    34381 ttgattatgt atccgcaaca aaagatgcag taaacaaatt ggcaaaaagc ggcaataaaa
    34441 aaatcgcttt tgtcagtggt gcgttgatcg atcccatcaa tggacaaaat cgtctgaaag
    34501 gctataaaga agcattaagc gaaaataacc taccatatag cgaaggattg atctttgaag
    34561 cacaatacaa cttcaaagat ggtctatctc tagtcgatcg gatccataat agcggtgcaa
    34621 cagctgtcta tgtttcagat gatgaattag ccatcggtat tttggatggt ttattagacc
    34681 gtggcgtgaa agtaccggaa gaattcgaaa tcatcacaag taacaattca ctactgacag
    34741 aagtagctcg tcctcgttta acaagtatca cacaaccatt atacgattta ggggcggtat
    34801 ctatgcgcct attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct
    34861 tgccttatgg aatcgaagaa aaaggatcta caaaataata aaaggacaac cattcagagg
    34921 ttgtgtgcct gccgtttaac tctgcgttac aaggagcaat ttcaaaaaac agcatctcat
    34981 gtttgatgaa tattgtgact cgtgattcgg agttggcagg agaacaccgt ttatttggga
    35041 aaaggggtta tgacaaaatt ttgttataat cctttttttg tgtcttaaaa aagagagaaa
    35101 gtggttgaaa ttacatttat cgtatataaa acaatccgtg tatagatgct gcattttgtt
    35161 caccaccatc aaaaaaataa gggactgtga caatactatt gtcacaatcc cttattactg
    35221 tgtattctat taaatcaatc atctttctca tctgattctt tagttgcttg attcgctctt
    35281 gcataagctg aagcggtgtt ccagtaatct gtatagtttt catcggtacc gccaacgcca
    35341 aatttaaagg cgcttttctt cggtccattt ttcgcccata gcgattcaac tgtttttcct
    35401 ggtgtttgga tggatttttt attgacagta acttttccag gtgtttgacc agtaaatcca
    35461 gatacttttt tcttcatcac atcagatgat aattcgaatt tctcatcagt gccaaaaatg
    35521 tcaggttttg cttggtaagc ttgattgatc agattggcaa gataattagc cgaacgtatc
    35581 cttgctttgc tgtccattcc agtattgtca tcgtgccctg cccagctact gatggtaata
    35641 gacggagtag agacaattag ccatgaatct cggtattcat tggtagaacc agtttttccg
    35701 acccaatctg cttttcctag atttccgttt aagcttgaga tggcatcttt gaatggtgta
    35761 gtgatttttg cattgatcac actacgcatc atgtcattca tgatcgatgc cgttgcttcc
    35821 gaatagatcc ggacagagtt gctttcgtgt ttataaatta catttcctgc gttatctgtg
    35881 atggaatcga tgatatagcc ttcttcatag acgccgccgt ttgctaaagc ctgaaatccg
    35941 ttggtctgtt gaagcgtcgt gacatctact gtacctaaag gagcagattc aacgccccaa
    36001 ttgtcatttg ccgggtagtt catctttgcc aagtagtgct cgtaagcata ttgtttcgat
    36061 ccaccggaat tcaacacatc ttgatataag tgataagccg ggatattgtt ggaccattct
    36121 agtgactcac ggacggtttg gaaggtgttc gatccttcgt tagtagcatt gacgattttc
    36181 tcgccagcat tttctccttc ttgccaagtt gttgcataat ctgatacacg ggattctgat
    36241 ccgataagcc ccatgtcgat tgcaggtcca taaacaagaa ctggtttgat tgatgagcca
    36301 gcttgacgtt ctgtatcgaa ggcatggtta ttttgattct gactgtaatt ccgtccgcca
    36361 acgaatccgt agatgcggcc agttctgtta tccatcaata catttccggt ttctacttga
    36421 ctgccagcac catcatctaa catgtatcca taattttcaa cgccattttg caatgcagca
    36481 tagacatctt tatcgattgt cgaatggatt gtatatcctt tattttggat cgtttgctgt
    36541 gccttttgat aataagcgtc actgaccgat gaatcagaca tatcagcgtt gtctttttca
    36601 gccaattgtt gtgcaatgat tcttgttgct tcattcatca cagtataata caagaattct
    36661 ctgtcagtct gttctgcgat ttgttgtggt agaaagtctt ttgtaaggtc ataggctttt
    36721 gcttcttcgt attgttcttt cgtaatttgg ccttcacgat acatactgaa taaaacaaaa
    36781 tcttttctgg caaggccggc agaaagatct tcttttagcg cgcctgtgtt cgtgtatgga
    36841 ctatacgtga tcgggctttg gggtaatcca gcgatataag cagcttgcgg cagcgtcaca
    36901 tcttttgcat ctacaccaaa gattcctttt gcagcttcct ggactccagc aatgttttgt
    36961 cccttattat tacgaccaaa tggtgagaca ttcaaatacg tagcaatgat ctcatctttt
    37021 gaaaagtatt tttctacttg tgcagaaaga atgatctcgt ttgctttacg tttgaatgtt
    37081 gtttcatctg taaggatctg ctgtttgact aattgctgtg tcaaagttga tccaccagaa
    37141 gagccaatac cagtagcttc agatactaat gctcgtaaaa cagctttagg aacgtaacct
    37201 tggtgtttat cgaagtattc atcctctgtg ctgataattg cagttttcaa taaaggtgaa
    37261 atcttatcag atgaaatagt cgtgcgcatc agatctgtct ggatttttga tatctctgta
    37321 ttatctgcat aaacaatctt tgacgtttct gtaatgtttc ctagttctgt ctgcagtgct
    37381 tttttagtcg gcaattgtgt gtcttcgacg agataggcaa aatagccagc gcctattcca
    37441 atacctagag ctgcgaacat caaaagaaga acagtaataa aaacagtcaa tgattgaaaa
    37501 actctaaaaa taatcttagg aacaaaccat ttttcttttt ttcttctatt ctgttttttt
    37561 cgagtatttt tgcgtggcaa aagggtcacc tcaactaatg gttttatttc gctaattata
    37621 gcaaataata gaacaaaaaa acagacaagg ctgtttttgg caggaatttc ttttttaaaa
    37681 gagttaaaac gtgagaaaat gatctttatt ataaaagagt tcttaagctt ttctttagag
    37741 aaaatttcag ttactatgga gaacagggca aagaaaaatc aatcattttc tttgtatatc
    37801 atcttaccaa tatataggaa ttgttagtaa caattgcctt tcaaggagga atccatccat
    37861 gttattaggc tcacacgtca gcatgagcgg aaagaaaatg ctgttaggag cagcagaaga
    37921 agcagccagt tatggatctt cgacttttat gatctataca ggcgcccctc aaaacactag
    37981 aagaaaagcc atcgaagaga tgaatattga tgccggaaaa gcgtacatgg cagaaaaagg
    38041 tctaacaaat atcgtcgttc atgcccctta tatcattaat ttggggaata cgatcaaacc
    38101 agaaatgttt ccttttgccg tccaatttct gagagaagag attgttcgtg cggaagcttt
    38161 aggcgctact caaattacac ttcacccagg tgctcatgta ggggcaggaa cagaagccgg
    38221 aattgctaga attattgaag gcttgaatga agttttgaca aaagaacaga cggcacaaat
    38281 cgctttggag acaatggcag gtaaaggtac ggaaatcggt cgaacatttg aggaattagc
    38341 gcaaatcatc gatggagtaa aattgaatga aaaattatcc gttacttttg acacctgtca
    38401 tacgaatgat gccggttata atattaaaga agacttttca ggtgtaatgg aagagtttga
    38461 taagatcatc gggttagatc ggcttaaagt actccatatc aatgattcta aaaatccgca
    38521 aggttcgcat aaagaccggc acgccaatat tggttttgga acaatcggat tcgacacgct
    38581 gaataagatc gtccatgatc caatgtttga caatatttcg aaaatcttgg aaactcccta
    38641 tgtaggcaaa gataaaaaga aagcttacgc accatatgga aaagaaatcg ccatgttcaa
    38701 agcgcaaaca ttcgatccag aagtgtttcc tgagttatta gcagaagaag tgatactcta
    38761 agattttcta tctgacagca gcaaaaaagg accaggcaca ttgccttgtc cttttttgtt
    38821 taagaaacaa aggagtactt tttgcagtct ttttcatcat gcatcttctc ccccctcttt
    38881 ttaaatttag acaaacaagg tatactggta gaaaagtaaa aaaacgaaaa gtttcacaag
    38941 caagtggcaa aacgtgattg gaaggacgaa aagatgctga acactttaaa acccttgatc
    39001 aaccaactaa aaaagaaaca tacatacttt attcttggca tagccctatt atttacaatt
    39061 attcgtttgt atttatatag taaagcaatc tatggaacag acatgtcggc aacctatgat
    39121 gaccagctgc tgattcgcta tgctcaaaat attttggacg gtaaatggct aggggagtat
    39181 acgactacaa cactttccaa aggaatctcc tacagcttat ttttagtact tgcacataaa
    39241 ctgcatcttt cttatgcagt tttgttcggc atgttcagta tttttgccag tatattggtt
    39301 gcactcgcgt ttaaaccgat tgtgaagaac aagtatcttc ttttaagtat ctatttttat
    39361 tttttgtttt ctccgattag ttttacacat gaatattcca ctcgaacata tcgtaatgcc
    39421 ctcgtgattc cggctgttga gatcattatc gcttgtttat tagctattta ttaccgcaaa
    39481 tacggttcta gcagaaaatt gctgccatgg tttttagggc tttctgtcgc ttttcctttc
    39541 ttttggttca ttcgcgaaga ttctatctgg cttcttcctt ttactgtagt agctttgggt
    39601 atcagtttcg tccagatctt gctagggcaa gacttttctt tcaaagaagt aaagaagtta
    39661 aaaaaacgac tctttcttct ctcgaaacgg gaatggctga aactttcatt gtttttactt
    39721 cctatagttg gcttttccat cgtctatcaa tggatcgaat ataaaaatga gacaacatac
    39781 ggtattgcaa ccatcaatga ccgttccagt ggagaatttg gacgtttgat gaaacaactg
    39841 atccgaatag atgatgaaac gaacttgaat aaagaaaaca gtgagatatg ggtctcacat
    39901 gctgcactaa ataaagcatt agccgcatct cctacattcc atactgtttc tacccgtatt
    39961 gacgaattat atacgactca cgcgtggact caaggtggaa aagtaaaaga attacccgga
    40021 gatatcatct tttgggcatt gcgagatgtg gtaagcgaat ctggttatta ccaaaacaat
    40081 gcaaaagaaa ccaatgattt ttggaaaaaa gtcaaccaag aattagaaga agcctatcac
    40141 aagggaacaa tcaaaaagaa aaaagagttc tatctgatgg gatcaggaga tggaaagacg
    40201 atgagtgatc tgccaatcgt attcgatttc cttcaatcag gatacgctta tgcatggcga
    40261 tataaagatt tccatcaagg aggaaattat agtttcggaa cgcaagaaca agtcaaacag
    40321 gctcaagaat tattaaacct tccgctaatg gagaattgga tcgatagtaa tgaaccgaga
    40381 acacctaatt tccaattaac aaaatcagca aaagtcgcga atgtcatcat aaaaatttac
    40441 caagttctgg ctattcccgt gctgattgct tcaatagcag ggatattact tatgatacta
    40501 ggaaccgtat ttgcaaaagc tgatcgtgcc tattatagtt cctctttgat tgtggtcacc
    40561 ggattaatac tgacacaatt ggtttttctt ttcggtgtct catggttttg ttcttatgcg
    40621 ccagaaaaaa aggactattt tttaagtgtc tacactggtg caggcgtgcc attaacgcaa
    40681 ttcatcgtag taacaagcat gctagttttc agtaaattaa atctgaaaaa agctttatct
    40741 tctaaataaa aagacccttg gtagctagat ttttaaccta actatcaagg gtctttgtgt
    40801 ggaaaaatca gtttttattc gatttcttct accgtttcct gcaaaagctg ttcatatttt
    40861 ttgtttcctt tatataaatc accaatcgtc catgccaaca ggagaaggac aagaacaatg
    40921 attacataag cgatgtgatc agccaatgct agttgagcgg aagtggcttg ctcgccgaag
    40981 aaactttcga tctgtcctgg taatcctcgt aagttcaagt aagttagact aatcacagag
    41041 aaccagccaa gaagttttac gatgaatgaa tttttaaatc ggctacccat ctccgcttgg
    41101 ctgttggtca tcatcagtaa aggaatcatc gaaaacggca acgcaaaagc caagaacacc
    41161 tgggattcat tcattaagtt attcaaagcg gcatgttctt cgatcgttcc ttttgtactt
    41221 gtaaacaaaa cacaaatcaa tacaggaatc acagaaagca atcgtgtgac cagtcttctt
    41281 agccaaatcg ggatacgcat atggatgaat ccttccatga tgacttgacc ggtcaacgta
    41341 ccagtaatag tagagttctg gccagaagca agaagtgcta cagcaaataa agtggacaag
    41401 ataccagatt ttgctacagc aatcaaaata ccattgctca tagctgatgg atcagataaa
    41461 gcttcataca gaccaaaaaa ggaaggatct tggacagaac cggttttgaa gacagcgaca
    41521 cccatgatca atagcagtgc attgactaga aaagccattg acaactggat attagaatcc
    41581 caagtagaaa aacgtacagc ttgtgctaca tcttcttcgt tttggtgatc gatcttccga
    41641 gtttgtgaaa ctgctgaatg aagatataag ttatgcggca tgactgttgc accaataatc
    41701 cctaaagctc cagtcagcgg agtttcgccg ccgatgaccg gtgagtctga gaatgtatga
    41761 ctgttaggga taaagccagc aaataaactg tgccaatctg ggttagacaa gccaacttga
    41821 tagacaaaaa cgaataaaat gacgaaaatt aagcagacaa ccagtgcttc gatcttacga
    41881 aacccgactt ttgtcagtag taagagaacg aaaacatcta atacagtaat aaagacggca
    41941 atcaccaatg gaatatcaaa aagtaagtag agggcgattg ctgcaccaat gacttctgca
    42001 atatctgttg ccataatggc taattcagtc aagatccata aaatgacacc taatgtctta
    42061 cttgtgcgtg ctcggattgc ctgtgccaaa tccatttggc tcacgattcc taattttgct
    42121 gccatatact gtaaaagcat agcaatcaga ctagacatca aaataactga catcaataaa
    42181 tattggaaat tttgtcctcc ggtgatcgaa gtagaccagt tacccgggtc catataccca
    42241 actgctacta acgcaccagg tccagagtaa gcaagaagtg ttttaaaaaa acttttcccc
    42301 tttggtacat caactgttcc atttacttca gctaaagata aaccatttgt atgttcaata
    42361 agtcgatgct tatgaaattg tttattggat tgattattca agaaaaaacc acctttccta
    42421 tttatctacg attacattct acacctcaaa aagaaaaaat taaagaaaaa gattcggtac
    42481 gcctaatttt ttttcatggc gcgtcttgct agatgacgaa agaaattttg ttctcctcta
    42541 aaaaaaccac tattttttgc atgaaactaa agaatacgcc tttctttttt caataaactt
    42601 tagtaaaata agtcattgag agcaattcgg aaatagaaag gagagattta tatgttatca
    42661 acaggtattg tcgtacttat tgctattatt gctttgctaa tcggcgcagt aggtggcttt
    42721 ttcctagcgc gtaaatatat gaaagactat ttagaaaaaa atcctcccgt taatgaggac
    42781 atgttgcgat caatgatgat gtcaatgggg caaaaaccat ctgagaagaa aattcgccag
    42841 atgatgcagc aaatgaagaa ccaagggaaa aaataggttc tgacccaacg actttttacg
    42901 ggctgatttt ttcagtccgt tttttatttc gtaatttgtt agactattca gattattcag
    42961 caaaaaagac aagcacagac aatcctaggc tcttgataaa gacaaaggag gagtaaaatg
    43021 tcgattttta gaaaattagg ctggtttttt cgtcaggaaa agaaacatta tttaattggg
    43081 gttttctcgc tattcgcagt ggccttggta cagttagttc ctccaaaagt gattgggatc
    43141 atcattgatg agatcgccga taaaaattta tcaataaaga tcattttcgg gtggatcttc
    43201 ctattgatta tcgctgcttt tttacaatat ctttttcgtt atatctggcg gacgaatatt
    43261 tggggaagtg cagctcgatt ggagaaagaa cttcggcagc agttattcaa acattttaca
    43321 gaaatggaca atctctttta ccaaaaatat cggactgggg atttgatggc tcacgcaacg
    43381 aatgatttga atgcgatcca gaatgtagca ggagctggta tcttgacctt tgcagattca
    43441 gtgattacgg gcggaacaac gatcattgcg atgattttgt ttattgattg gcgcttaaca
    43501 ctgattgcgc tgattcctct gcctttgctg gccgtcactt cacgagtgct ggggtcaaaa
    43561 ctccatgatg cttttcgtga ttcgcaagaa gctttttcta gtatcaatga taagacacaa
    43621 gaaagtatca ctgggatcaa agtcattaaa acatttggac aagaaaaaga agatctagcg
    43681 gattttgaag aaaagattga agatgcaatt attaaaaaca aacgagtcaa ttttttagac
    43741 tccttatttg atccattcat taccttgatt atcggtctgt cttatgttgc aacaattatt
    43801 ttaggtggaa agtatgtctt ggaaggcacg atctcgatcg ggcagatggt ctcatttatt
    43861 agttatatcg gtatgcttgt ttggccgatg tttgcgattg gtcgattgtt caacgtattg
    43921 gaaagaggaa atgcttctta tgatcgcgtc aatgaacttt tgcatgaaaa gacgcatatc
    43981 gtggaacaac tgggtgcagt gcagaccccg gcaaaaggcg agctgaatgt agctatttcc
    44041 tcttttaatt atccgaaaga agaatcacca gcattagaac aaatcaagtt tgcgatccaa
    44101 gaaggagaaa cattaggtat tgttgggaaa actggtgcgg gaaaaacgac gatcatcaaa
    44161 ttactgatgc gtgaatacga tgattatcaa gggaaaatcc aatttggcgg acgttcgatc
    44221 aaagaatatt cattgaatgc tttattagga tcaatcggct atgtgcctca ggatcacttc
    44281 ttgttttcga tgaccattcg agataatatc cgtttttcta atccaaaact ttcagaagaa
    44341 gaagtagagc atgcggcttc tttagcattt atcaacaatg aaatcaagga attaccaaaa
    44401 ggttatgata ccttagttgg agaacgtgga gtctctttat ctggcggaca gaaacaaagg
    44461 atttcgattg ctcgagcatt gatcaaaaat cctgaattat taatattaga tgatgcactc
    44521 tctgcagtag atgccaaaac agaagaagct atcttgcata atttaaaaga aataagaaaa
    44581 gagaaaacaa ctatcattgc agctcaccgt ttaagtagcg tgatgcatgc aaaagaaatc
    44641 attgttgtcg atcaagggaa aatcattgaa cgaggaaccc atcatgagct gcttgcacta
    44701 ggcggctggt atgaacaaat gtgggagaaa cagcaattag aagcgaagat agaaggaggc
    44761 gaggcctgat ggatgaaaat tatcaatcgg aatggacaaa atcgattcca cttaaagaac
    44821 agaaaaagat catcaaacga ctgatgaagt tcgccaaacc ttttagaaga acgttcatcg
    44881 ttgctatctt atttgcgttt gctttgtctg tgatcaatgt cctattgcca aggatcatcc
    44941 aaacttttat ggatgatcat ctggctaaac agtcggctac gacacaagtt attttgtttt
    45001 ttgctggtat gtacttattt ggagtcatag taaaaagtat catttggttt ttccaatggt
    45061 atttgtattc catggcctca ttgaagacct atcagtatat tagggtaaaa ttatttgaaa
    45121 agctgcatac actaggtatg cgttattttg atcagacacc ggcaggatca accgtttctc
    45181 gagtgacgaa tgacacagaa acgctatttg aattttggta cgtctttttg atggtattga
    45241 ctgggatctt tgcagtcgtt tcttcattcc ttgccatgtt tcaaatcaat ggagaaattg
    45301 ccttgtggac tttggtgttt ttgccgattt taggaatcgt catctggtat taccagaaat
    45361 tcagttctcg tatttaccga aatatgcggg aaaaattaag tcagctgaac acgaaattga
    45421 atgaatcgat ttccggcatg cgtattatcc agcagtttcg ccaagaagct cgtttagcta
    45481 aagaatttga acaagtgaac aatgagtatt tagacacgcg ttatgcgatg atcaaaacga
    45541 attcgattct gttaagtccg atcatcaatt ttctatatgc attggcgatt gcgttggtgt
    45601 tgactttgtt tgggatcgat gcgttgcatt ctcccgtaga agttggactg atctatgcat
    45661 ttacgaccta tgtccaggga ttttttaatc caatgtctca aatgatggac tttttgagca
    45721 tcttcacaga tgggatggtt gctggtagtc gtatcttgaa gattatggat acggaagaat
    45781 atgcaccaca acaaaaccct ggagcagatc aaatgattac ttcaggtaag attgagtttc
    45841 gcaatgtaag cttttcttat gatggagagc acaatgtatt aaacaatatc tcttttgttg
    45901 ctaatccggg agagaccgtt gcattagtgg gacacaccgg aagcggaaaa agctcgatca
    45961 tcaacgtatt gatgcggttt tacgaatttt atgaaggtca gatattgatt gatgatcatg
    46021 acattcgaga atatccaatg gaagagttac gtaagaaaat ggggctggtc ttgcaagatg
    46081 ctttcttgtt ttatggggat atcgcaggaa atattcggat gatgaatcca gctatcacag
    46141 acgaacagat acgccatgcg gctgagtttg ttcaagcaaa caaattcatc gatgaattac
    46201 caaaaggata tcactcaaaa gtgatcgaac atggtgccag ctattctagt ggacaacgac
    46261 agctgatcac ttttgcgcga acaatcgtga ctgatcctaa aattctagta ttggatgaag
    46321 caacagcaaa tatcgatact gaaacagaag gattgatcca agaaggattg gcaaaaatgc
    46381 gtcaaggacg gacaacgatt gcgattgccc atcggctgtc aacgattcga gatgcagaat
    46441 tgattttagt tttagacaaa ggtcatatag tagaacgcgg gaatcatgaa acacttctag
    46501 cgcaaaatgg tttgtatgca gatatgtaca aactgcaaag cagtggtata taaaagatgt
    46561 tgtgacagta gaattttgac ctaagcacta gagtgaaaat aaaacatagc ttctcatatt
    46621 tttcatctgt gagagttagt gtcgtagtca ccagatttaa aaaaagattc gacagctagt
    46681 cagaagtgcc taatagccac taagaaagcg ttgacaaaag catttgcttt cgataatata
    46741 aagaaaacga tacaacttgg atagtgattg cctgatgcaa gctaaacctg atgagtctcc
    46801 catggtttca tggacggctt tttgggtttt tctttttgtt ttggatacag ccctatacat
    46861 catggaaagg agcaaaacat gctgattgag aaagtactga ataacaacgt cgtaataacc
    46921 agggatgaaa gaaatcagga aatgatcgtg atgggaaaag gtttggcatt caaaaagaaa
    46981 gtgggtgacc cgatttttga ctcatgcatc gacaaaaaat ttcagttgac aaatgacggg
    47041 ctttcacaac agttccaaga attggcacaa gcgattcctc ttgagtactt agaaatttct
    47101 tgtgaaatca ttgaatatac aaaagaggct ctacaagtcc agttgaatga ttctgtgtat
    47161 attgcattga cggatcattt gtatacggct atcgaacgtc acaaaatggg aatatttgta
    47221 ccaaatgtat tgctgtatga tatcaaacaa ctatttccta aagagtatgc catcgggaaa
    47281 aaaacagtga agaaaatctc ggataaatac cacatcaagt tatcagaaga tgaagccgga
    47341 tttatcgcat tgcatttagt caatgcacaa ggcattcaaa gccagcaaga caatacctac
    47401 ctgtttacta taacaataca ggatattcta aatatcgtcc gctattacta taagattaca
    47461 tttgatgaag attcagtcca tttttttcga ttcgtcacac acttacgttt ttttctgaca
    47521 cgcgtcctag atggagaaaa atcagaagtg ggagaagttt ctgaggaatt gtttcaggtt
    47581 attcaaaaaa aatataagga tacgtttggt tgcgttgaga aaatagcaga ctttttagcc
    47641 gagacgttca actaccagat gtccacggac gaaaaaatgt atttaacgat ccatactgcg
    47701 cgtttagtgc atgagaatcg ttgtaaataa aaaaaggagg ataaattaac aagaacgtat
    47761 gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg gagggaatag
    47821 aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc gggaaggaaa
    47881 atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa aacgaagaaa
    47941 aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg cgagcgggag
    48001 ggcagtatca agttgtcatt gggaatcatg tgccagtcgt gtttgaagag gttataaaag
    48061 caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg tttgatatgc
    48121 ttattgatgg aatatcgggc tgtttccagc cattccttgc tatcctagct gctggcggga
    48181 tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc ggttctggca
    48241 catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct gttattatcg
    48301 gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg cttggcgcag
    48361 cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa gcaccactaa
    48421 cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta ttcggcattc
    48481 cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt gtgttgttag
    48541 ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg aatttcttcg
    48601 ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt ggaccagtat
    48661 tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca ctttcgccag
    48721 ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt ggtctccatt
    48781 gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa aatattatga
    48841 cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc tactttaagc
    48901 ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt attgtgggga
    48961 ttacagagcc agctatttat gggtttacat taccacgtaa aaaaatattt atttttacct
    49021 gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact tcttggaatc
    49081 aaggtggact aggcatcttt acaattccca actatattag accagatggt gatttaacag
    49141 atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt acgctgactt
    49201 ttttcttttg gaaggatgaa gcaggtgaaa cagatgatat acagaaaaaa tcaggtaaag
    49261 aaattgtaaa aacaccgata caaggacaaa tcgctccact aaatgcagca aaggatgcag
    49321 cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa ggagaagtac
    49381 gtgccccttt tgacggaaca gtcatgaccc tgttcccgac gaaacaggca attggaatgg
    49441 tttctgaaac agggctggaa ttgctgattc atgtcggatt ggatactgtg cagttggaag
    49501 ggaaatattt tgaaagctta gtgcagcaag gataaagagt gaaacaaggc gatctgttgc
    49561 tacgcttcga caaaaagaaa atcgaaaagg aaggatacag tttagaaaca cctgtgatcg
    49621 tcaccaatta cacggattat ttggatattt tggaaaacca aggggagacg gtcgggccag
    49681 acgatgcact tatcaccgca ctgacctgaa cggttaggtg aagaatggac taggcttaac
    49741 tattcttttt attccacttc tttattttca aaattgaaag gctctttcat gatggctgag
    49801 ctgatagggc gtttatcgaa tttttgaccg acagaaatta gatggactaa attctgttga
    49861 taattttctg caaaaacaca actatataac acgtctagaa gataacaaat tgatgtattg
    49921 atcgtaaaat taccaatttt tgaatacagt cgctcgcgtg tcgtaagtcg caaagcggct
    49981 gtactcattg cagatagcgt tgaatcaccg atactggtca aagctaaaat tggaacatga
    50041 ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt ataagaaatc
    50101 agaatcccgc acatatcact cgtgcaattg tacgcttcat agaatgattc accaaaagtg
    50161 gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc ttgagaaatc
    50221 aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat tagttgtttg
    50281 gctttttgca gttggtcatg atgaatgaga gaaagtgtat catgaatcgt catctgttct
    50341 aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa aggaagattc
    50401 gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa agcattacga
    50461 aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt agaggggtgg
    50521 acataacatc gctcagcaat cgcttggatg gtcatttcgc caagtgctgg agaatcgctt
    50581 aagagaaact ggatcacttc attttctgaa ggagaaaagg agatttcttt gattttttca
    50641 cgtaataaca tgtagatacc ttcttcttat ttttttacgt aaattataac ttttttgaaa
    50701 atgatttgta aacggattta tactatataa cttaaaatag acattttttg acaagatatt
    50761 tacaaaaatc cactagaaaa cagtaaattt tgtaaatgtt actggaaaga aacagatttc
    50821 tattgtttgt tttgtttaca aaaaagataa tgaaagtgta aaaaagtaaa ggagtgagaa
    50881 catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca cccccgagtt
    50941 gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct ggctcgtaga
    51001 tgtagaagaa ggaaaagaaa agcttgagat cgttagtcag atgtctcagc gtatgtggga
    51061 tgcttctccg tatgaagtca agatctatgc aactctggat cgggaagaag cattgaaaga
    51121 tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa tcaaagacga
    51181 gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg gcgggatatt
    51241 caaagctttt cgtaccgttc cagtcgtttt ggcaatcgtt gaggacatga agcgtttatg
    51301 tccaaatgct tggctgatca atttcgcgaa cccaagtggg atggtgacag aagcagttat
    51361 cagatatggc aaatgggata aagtgattgg attatgcaat gtgccagtga tggcgatgat
    51421 gacagaacca gcaatgcttg gcaagaaaaa agaagagctg atctacaagt ttgccggtct
    51481 aaatcatttc cattggcaca aagtagcaga taataaagga aatgaccgca cggcagaatt
    51541 gattgatcgt ttatacgatg aagagaatgg attaccgaaa aatatctttg atatcccgtt
    51601 tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc gctattacta
    51661 tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat caaacgattg gtacaagagc
    51721 gcagcaagtg aaagaaacag aagcagagct atttgaatta tacaaagatc cagaattaga
    51781 ccataaacct gaacaattgc aaaaacgagg tggcgcttat tattcagacg ctgcctgtga
    51841 aacgattgct tcaatctatg gaaataaaga aacacagatc gtggtatcta caaagaatga
    51901 tggagctgtt cctgacctgc cggcagattg tgtagtagaa gtcactgcct atgttggcgc
    51961 acaaggagcg agaaatgtag cgtttggttc tcttcctgct gctgaaagag gctggttgca
    52021 agtaatgaag aatatggaat tgctgacgat cgaagcagct gtgactggtg attatgggac
    52081 agctttacag gcgtttacga tcaatccact tatacgatca ggaaagacag caaaacggat
    52141 catggatgag ttgtttattg cacaccagca gtatttaccg caattcaagg agacaatcag
    52201 ccgattaaaa gaagaaggaa tcgaagttca agatgaactg gcaagagaat tatcaggtaa
    52261 tacacatata atggaggaag tataatggga ttcagaaaag attttttatg gggcggtgct
    52321 acagcagcca atcaatgtga gggcggttat gatgaaggtg gacgcggact agcaaacgta
    52381 gatgttgtgc caatcggaaa agaccgtttt cctgttatca ccggaaagaa aaaaatgttt
    52441 gaatttgatg aggatcattt ttatccagca aaaattgcaa tcgatatgta tcatcgttat
    52501 aaagaagata ttgctttgtt tgcagaaatg gggttcaaga cttatcgaat gtccatcgcc
    52561 tggagtcgta tttttccaat gggggatgag aaagagccga atgaagctgg cttgaaattt
    52621 tatgaggatg cctttaaaga atgccaaaag cacggaattg agccacttgt gacgattacg
    52681 cattttgatt gtccaatgca tttgatcaaa gaatatggtg cgtggcgttc cagagaactc
    52741 gttggttttt atgaaaatct gtgtcgagtc atcttcaatc gctataaagg acttgtcaaa
    52801 tattggctga cattcaatga gatcaatatg atccttcatg caccattcat gggcgcaggg
    52861 ctgtattttg aaaaaggaga aaatgaagaa caaatcaaat atcaagcggc acatcatgag
    52921 ttggttgcaa gtgccatcgc aacgaagatt gctcatgaag tagatccgga gaacaaagta
    52981 ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga agatgtttgg
    53041 gcttctagaa aatcagatcg agaaaattat ttcttcattg atgtccagtc acgaggagaa
    53101 tatcccgctt atgcattgaa agaaatggag cgaaatggca ttcagatcga aatggaacca
    53161 ggggatgccg cattgctgaa agaacatact gttgatttta tctctttttc ttattatgct
    53221 tcccgagttt cgactaccga caacgaactc ttggaacaga ctgcaggaaa tatctttgct
    53281 tctgtcaaaa atccatatct gcaagcaagt gaatgggggt ggcaaattga tccattaggc
    53341 ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccattgtt tattgtagaa
    53401 aacggcttag gtgcagtcga tacgccagat gaaaatggtt atgtggaaga tgattaccga
    53461 atcgattacc tggcagcgca tatccaggcg atgaaggatg cagtagaact agacggagtc
    53521 gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg taccggtgag
    53581 atgaagaaaa ggtacggatt catttatgtc gatcgtgata accaaggaaa tggcacatta
    53641 gcccgttcaa agaaaaaatc attcaactgg tataaaaaag taattgattc aaatggagaa
    53701 aatctttcga acgattgatc aatgatttat aaaagtctat tgtttttcag cggatttttc
    53761 gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt tcgcattggc
    53821 tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga tgtaatcgtt
    53881 attggcgcag gtcctgctgg gatgactgct gctttatatg cttcacgttc aaacttatcc
    53941 gtattgatga tcgaacaagg tgcccctggc ggccaaatga acaatacggc agaaatcgaa
    54001 aattatcctg gattcgattc gatcatgggc cccgatctag cttataagat gtatgaaagt
    54061 gtctcaagat ttgggacaga aaatgcttat ggaatcgttc aagatatcaa ggatcacggc
    54121 agccataaag aagtcatctg tgaagataaa agttacgaag gaaaaacagt cattatcgca
    54181 acaggatgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc tggacgaggc
    54241 gtttcttact gtgcagtctg tgatggtgcg tttttccgta acaaacgtct agtagtcatc
    54301 gggggtgggg actcagcagt agaagaagcc atctatttga cacaatttgc ttctgaagta
    54361 gtgatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga ccgtgcattt
    54421 gcaaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt tggaaacgaa
    54481 atggtagtga caggtgtgaa agcacgtaat gtcaaaaccg gcaatgaatt tgatttagca
    54541 gcagacggag tattcatcta tgttggactg gatccattaa ctgcaccttt tagagaagca
    54601 ggtttgacga acgaagctgg ctggattcca acagacaacg aaatgaaaac gaatatgcca
    54661 ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac tacagctgtt
    54721 ggtgacggcg gtattgctgg acaacaagtg tataaatact tggagtcttt agaagaaaca
    54781 acagcataag tttaaaaaga gtagaagaac gggttcaatt gttcttttac tctttttttt
    54841 cgcgtgaaat ttactgaatc tgatttatgc gaacacgtcc aacacctttt gataaaagaa
    54901 tcctttcttg atttaagcgt tttttggtcc tcatactttc atatctaata agtgaatgct
    54961 atacttatta agaaccatat ttcagaaaga ggtgttattt tcatgtcttg ggaacaagtg
    55021 tatgaacaat gggtacatga agaaaattta gaagaaaact taaaaaaaca attaaatgat
    55081 ctgggtgaag atcctgaaaa acttgaagat gctttttatg caccgttgga attcggtact
    55141 gcaggtatgc ggggaatctt aggacctggg atcaatcgaa tgaacatcta tacggtaaga
    55201 caagcaacag aaggactagc acgtttcatg gatacacaag gtgaagaaac aaaacgccga
    55261 ggtgtcgcta tcgcttatga ctcacgccat cagtcaccag aatttgcaat ggaagcagcc
    55321 aaaacactag cacatcacaa tatcccgtct tatgtattcg aaagtctgcg tccaacacca
    55381 gagctttctt ttgcagttcg tcatttgaag acgtttgcgg gaatcatggt aaccgcatct
    55441 cacaacccag cggcttacaa tggatataaa gtctacggag aagatggcgg acaaatgcct
    55501 cctgcagacg cagatgcatt gacgaaatat gtccgtgaag tcagcaatcc gctaaaagtc
    55561 gaagtattat ctgacgaaga agctaaacat agcgggttga tcacgatcat cggagaagaa
    55621 gtggacgctg cttatctaga agaaatcaaa gcagtgacga ttgatcgtga attggttgaa
    55681 acaatgggga aagatttgaa acttgtgtac acgccacttc atggaacagg gaaaatgttg
    55741 ggcgaacgtg cgttgaaaca agctggattc gaacaattcg tactagttcc cgaacaagct
    55801 gtggcagatc cagatttcac tacggtcaaa tcgccaaatc cagaagaaca ttcagctttt
    55861 gaatatgcta tacgacttgg agaaaaagaa ggagctgact tgctgattgc aacagatcct
    55921 gatgcagacc gtttaggcgc agcggttcgt ttgccagatg gttcttatga agtattgact
    55981 gggaaccaaa tcggagcatt gtttgctcaa tatattttgg aagcacatca atcaagaggc
    56041 actttaccag aaaacgcagc ggtattgaaa tcaatcgttt ccagcgaatt gcctacagca
    56101 attgcggaac actacggtgc taccatgttc aatgtgttga cagggtttaa attcattgct
    56161 gaaaaaatcc aacaatttga agaagaccat tcacatactt ttatgtttgg attcgaagaa
    56221 agttatggtt atttagttaa accttttgtt cgcgataaag acgcgatcca agcattgctt
    56281 ttattagcag aagtagcagc ttactataaa aaacaaggca aaacgttgta tgacggcttg
    56341 caaacgattt ttgaaaccta tggctatttc gcagaaaaaa cgatttctgt gacaatgagc
    56401 ggacaagaag gttctgcgaa aatctctgct ttgatggaaa aattccgtaa agaagcaccg
    56461 acagaatttg ccggagcaaa agttattcaa acagaagact ttaaagaatt gacgagaaca
    56521 gaccgtgaag gaaacgtaga aaaactaaca acaccgccat ccgacgtatt gaagtataca
    56581 ttggaagaaa atggctggat cgctatccgc ccttcaggaa ctgagccgaa aatcaagttt
    56641 tatattggtg taaaaggaag ctctacagaa gacgcagccg aaaaagtagc ctcttatgaa
    56701 gcgattatca aaaagatgac agaagaataa aaaacttgag actgatggaa aaagtggtaa
    56761 actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac acgcaaaaat
    56821 caaaagtaga gaaagtcagt caattattca aaatgttgag tgatcccact cgtcttaaaa
    56881 ttctgctata tctgaaagac ggggaacaaa atgtgacagc aatcacccaa gcagtggaaa
    56941 tggagcagtc tgctgtatcg catcaactac gtttattaag agaaaatcat gtcgtcaagt
    57001 ctcatcgcga gggaaaagcg attttgtata gtcttgatga ttctcatgta ctggatattt
    57061 tgaaccagac actcaaacat gtggaacatc aataacgata ggtgaaaata gataaacact
    57121 ggagtgtgac aacgaagaaa tttcgttgtc acactccagt gttttttaag aaaaagacaa
    57181 aaatgattaa tcgctttttt ctgttgtttc agttccataa taactatgat cttctaggtc
    57241 taaagcattc aaaataagcg aagccgtttc ttcaatagat aaagatgcca cattgatcac
    57301 tacacatcct agtttttcat aaagatcatt cgcaaaatcg agttcagctc taattttttc
    57361 gatatcggaa taagcagtat cagggtttaa tccgtaagag cgcatccgtt ctttacggat
    57421 attatttaaa atatctgggt tattcgttag tcccacgatt tttttaggat cgatttccca
    57481 cagctgtttg ggaatatgtg cttggggaat caaaggtaaa ttagcaactt ttaaattttt
    57541 atttgctaaa aacaagctca aaggcgtttt ggaagttcgg gatacgccta gcaaaacgac
    57601 atctgcttct aaaaaaccac gaggatcttt cccatcatcg tattttacag caaattccat
    57661 tgctttgatc cgtttgaaat agttctcatt caataaatgg agggcaccag gttgccgaat
    57721 tggcttgata ccagttaaac gttcaatttc tgatatcggt ggcgtcatca catcgaaact
    57781 aaataattga tgttcttcac aaaaagaacg tgttatctcg atcagttctt gattgattaa
    57841 tgtatgcagg acaagtgctt tttcttctaa tgcatcttgc aatgcctgcc ttaatgtttt
    57901 ttcatctgtc acgaacgttc tgcggaatag gttgaactca acggtaggat actgagccat
    57961 agtagcttgc gctagttttg tcgcagtttc tccagcagag tcggagatga caaaaaaagt
    58021 aacgatttct gtagaatcgg aagacatgga taaaacctca cttgcatttt ttttatgttt
    58081 attataccat actctagaat gcgtacttcg taatcatttt gatttggagg aataagtcat
    58141 gaaaaaacag acattgatcg aacaatcatt agaaacattg aaaaataacg gttttaaata
    58201 tactaaaaaa agagaagcat tgttaactta tttagtgaag cgtaaccgct acgtatctgc
    58261 taaagaagtt tttgactata tgaatcagga attcaaaggg gtaagttatg atacgattta
    58321 ccggaatctc catgattttg cagagatcga tttactggaa gaaaccgatt taaatggtga
    58381 aatgaagttt cgttttcatt gctgtcaggg ggaaatcggc catcaccatc attttatctg
    58441 tacagtgtgc ggaacaacga aagaaatcca tatgtgtcca atgaattatt tcgaagaaca
    58501 gctaggtgga tgcacgatcg aagggcatag attcgagatt ttcggaagat gtgaaaattg
    58561 tcaataagga tttaaataaa ataacatttg ataatttaaa aaaaaagcaa gatttccttg
    58621 acggtcaaaa cagcattaga tataatattt acaggacagt cttgtttttt gagatatatt
    58681 tcaataacaa ttacggttac tttttaagct cggagggagg gaattaacat gtcaaaaaca
    58741 gtcgttcgca aaaacgaatc acttgacgat gctcttcgtc gcttcaaacg ttccgtgtca
    58801 aaagcgggta ctttacaaga atctcgtaaa cgtgaattct acgaaaaacc aagtgtaaga
    58861 cgcaagaaaa aatctgaagc agctagaaaa cgcaaaaaat tctagttctg tgatgaattg
    58921 gagttggtag tgtgtcacta ctaagtagat taaacgatga catgaaagca gcgatgaaag
    58981 cgaaagataa ggaatcttta caagtcatcc gaatgatcaa gtcatctata caaaacgagc
    59041 agattaaaga aggtcacgac ttgacagaag aagaagaact aacggtgttg tctcgcgaaa
    59101 tgaaacagcg acgtgattct ttgcatgagt ttgaagaagc aggacgtgac gatttagctg
    59161 aaaaagtaaa aagtgaaatt gtaatagtcg aaaaatatat gccggaacaa ctttccgacg
    59221 aagaaattcg acaacttgtt caagaagcaa tcactcaaac tggtgcttca tcaatgaaag
    59281 aatttggcaa agttatgggt gcaattatgc ctaaagtcaa aggaaaagca gatggaaacc
    59341 aagtgaatgc gattgtgaaa gaactattac aatcttaatt ttatcattta aactgcggac
    59401 agtgattgtc cgcagttttt tgtgttcgta aaaaaagaac taaaaaacag ctagaagaaa
    59461 gaataagatt ctttcttcta gctgtttttt tcctgtttaa tatctcttat gttaaattgt
    59521 gtttgatttg gttattcttc tggtagttca tttgcttctt gtttataccc tgataggcga
    59581 taggcgtaat aaaagagtaa gctgccagcg taaccaagta acgttgggac catccagcca
    59641 agtcctaatg cagcaaaagg aacgtgatga tgataaaatc ctagtaagct tgctacgaca
    59701 gtttgctgac tcactaatgc aggcgaattt gccaacatat ccaatacggc tggaacggca
    59761 gaaaagaaca tcgttacttg atagacaaat ggactctttt ggaagaattt tgctgttaag
    59821 gaaagcaaga ttaacgcaag tgacaatgga tacaaaagca tcaatactgg taaagaccat
    59881 tggatgatat tatccaatcc ggcattcgca acgacaaaag agacaaaagt cgttaaccgc
    59941 agccatgtca tataactgac tttagggaac agtttatgga aatcttgtgc aaaagaaaca
    60001 acgagtccca tagcggtagt gaagacacct aatgtgacca ttacccctaa gaagctagca
    60061 ccgaagttac ctaaataaaa attagtgatc tgagaaagtg cagttccgcc attttctgat
    60121 aaaggcatct tattcaaact catcgcaccg agaaggacca gaccaaaata aatcatgact
    60181 tctaacgcaa cgctcaggct accagctttt gctgttaatt ctgtgacttt tttatcttta
    60241 aaaccaagat cacgaactgc atggacaaaa gtgacactta aagcaagtaa agctactgca
    60301 tcaaccgtgt tgtatccttc taaaacgcca tttgtcaaag cgttcgtctg ataagaatct
    60361 gcagcagatt ggtttagatt ccccatgggt tggaataaag aaacgacaaa aatgatagct
    60421 aataatgcaa gaaacaagga gtttaaatac tttccgacat atttgaccaa acgtgttgga
    60481 tgtacagtaa ggaaataagc aagtaaaaag aaaagagcag agaataaaaa catacctaat
    60541 gaatcatatt tttcaggcaa aaagggttta gcagccattt caaaagcagt tgctgctgtc
    60601 cgcggtgtac caaagaatgg tccgatcgtt aaatgaacaa ggatcaagaa caaggcagca
    60661 taccattttc cgacaggttt agctaagtca tacagaccat cactttttgt cactactaca
    60721 gctaagatag ctaataatgg gaaaagagcg cctgagatgg caaatcctac accagcgctg
    60781 atccagtttg atccagccat ttgacctaag tggacaggaa agatcagatt acctgacccg
    60841 aagaacatcc cgaagataag tgaactgacc atcaataatt gcttccatga ataagttttt
    60901 gtttcttcca taaatttttc ctccaataat tatcattttg agtaacaaaa taaacgttgc
    60961 agaaactaga aatgccacta atatcagatt ccatgaaaac caccacctta aaatataaaa
    61021 aaaagccctt ttccacttgg gaaaagggct ctagattcta gaacggtacc accttttttc
    61081 gcttaaaaaa gcttcttcat tcacggacgc ttttcgataa agttatccgc tagcctgata
    61141 atggaggcag ccacaatgat ctacgttttc gacattgact cggaaatgat gttcgtctat
    61201 tcgatttatt gtcatttctc acttacatga ctcactggga ataaatcgct gaatagccta
    61261 ctgttttcct cgacgctttg ctgtatattt atttgttgac gttataatac acctgttttt
    61321 tttgaaatgc aagaatattt caaatatttt gttttctttt tttgaaacaa gaaaaatacc
    61381 cagagaatac ttgctaagtt agacggaata cttgtcctct atcttatgca tagcttacat
    61441 gatcagcttt ttttattcaa acgagacctt tagagaatgg aagcagctat tttttaacgt
    61501 ttcttggtac ttatggttaa gcaactcttt tagaaccttt tcttatttgc tatatagttt
    61561 cttctgagta tggtaaaata ggaaagaatg aatcgaaaaa aaggggatgt ctatttttga
    61621 cggaagagtc tagttcttta gagattagat tgacggctgc tgatgatgtc agcatgcttt
    61681 taggtgcaca cgacaagcat ataaaactga ttgaagaaac aacagaaaca accatccata
    61741 cacgtggaga aatcattcag atcattggcg aacaatccca gatttccttg gctgcctcag
    61801 tcattcggac attgcaggaa ttgatcaaac gaggaatcca tgtaagtaca ccagatgtcg
    61861 tgacggcact gaaaatgggg caaaaaggca cattagatta ttttgttgaa atgtatgagg
    61921 aagagatcgt caaagaccgt aatggaaaac caatacgggt aaaaaacagc ggccaaaaga
    61981 aatatgtcga atcggtagcc aaacatgata tcgtatttgg cgtaggacca gccgggacag
    62041 gaaaaacttt tcttgcagta gtattagcaa tcgctgcctt gaaaaaaggt gaagtgcaaa
    62101 aaatcatttt gacgcggcct gctgtggaag caggggaaaa tctaggcttt cttcccgggg
    62161 atctaaaaga aaaagtagat ccttatctgc gtcctgttta tgatgcttta taccaaattt
    62221 ttggattaga tcatacgaac cgattgatgg aacgaggcgt gatcgaaatt gctcctttag
    62281 cttacatgcg ggggcggaca ttggatgatg cattcgttat tttggatgaa gcacagaaca
    62341 cgacagttgc tcagatgaaa atgttcctga cacgtcttgg ttatcaatca aaaatgattg
    62401 tcaatggaga tacgagtcag attgatctgc ctcgagggac aacaagtggt cttgttcatg
    62461 cagaacgaac attaaagcaa atcaagaaaa tcgattttgt taattttgaa gcaagtgacg
    62521 ttgttcggca ccctgtagtt gccgaaatca tcaaagctta tgaaaaggca gatctccatc
    62581 aagaataagc tttgcttagg aggataagca catgttgaat aaaacactgc agaaactaca
    62641 agaaaaactt ggaaaggcag cattgccttt cctgctgttc ttgtttttta tcattatctg
    62701 cggcgttaca tttagtagtg tcaaacaaaa ggccaatgat ttcagagaag gccaagtagc
    62761 agaagaaagt atccgtgcga acaaaacgat cgagaacacg gaagaaaccg aacaaaaaag
    62821 aaaactggca gcagaagcag tcactccaga atacacttac caaaaagatc ttgcagatga
    62881 ccagaacaat cggatcaagc aactatttga attgatcgat aaaacgaatg atgccatcaa
    62941 caaatcttat gacgaaaaga ttgataaggc aaaggaagat gaaaatatcc caaagcctac
    63001 tacagaagag cggatcgccg cgttgaagaa aaattttgaa aatgtgaatg cagaaaatgt
    63061 tgcgttttat caaaaactac cgaacaattt ttattcgacg atatttgaaa tgactgaaac
    63121 tgagatccat actgtgaggg atgagagtct gaagctcatt gacgaacaga tgagcaaaca
    63181 agttcgtgaa agtgaattgg aagcttttaa acaagaagca gaagagcaaa tccaatattt
    63241 gaatgtcaca ccagcacaac aacaaatgat tcgctactta gtggatcaag gtatcgtagt
    63301 gaatgacgta ttgaatgaga aaaagacaga agaattgaaa caatccgcac gagaagctgt
    63361 acaaccagtg atgatcttcc aaggagaaat cattgttcgt gaaggtaatc aaatcgatgc
    63421 ttcagctatg aaaaaattgg aattactagg actgactaat caaacaactt cgatttttcc
    63481 gctaattgcc atggttttag ctgttttgct tcaaatcggc gtactgattt ataattcact
    63541 tcaattccaa aatgcaggag aaagaacaaa atacgtgctt ttctatgtga cagctatgtc
    63601 agtcagcata gtgctgatga aatttttcca attattccaa acggaacaag ctacttatat
    63661 cccgctgttt tatcccgctg cttttgcacc acttgtttta agctttttct tgaaccgaag
    63721 agcagggatc atggctggta ttttccaagc agtatctgca cttttcattt tttatgattc
    63781 gattggcacg aactttctaa cgattatctt gatgtcttat gttttctctg ggctcatggc
    63841 aacgattgtc agacgcaaac ggatctcaga acaaggatta tctgctgcca tgtgggtcat
    63901 tatttttcct gtgatgatgg atgtcatttt gatcgtctat caaggaatga gctttggtga
    63961 tgcaacgact tggttaatgc ttgtctgcgg gttcactggt gcactcttct cattcttgct
    64021 gacgatcggt ctacacccat atattgaatt gatggtcaat gatgacagtg taattgtatt
    64081 aaatgaactc agcaatccaa atcatccttt attgaaacag ttgctggaag aagcgccagg
    64141 tacttatcat catagtatga tggtagctaa tctaagtgct aacgctgtag cagaaatcgg
    64201 cggacgttct ttgctaactc gagtagcttg ctattatcat gatatcggca agatcaaaca
    64261 tgctagcttt ttcgttgaga acttgccttc aggagcggaa aacccgcaca atttcttatt
    64321 gccagaagac agtaaacata ttatctttgg acatgtgaca gatggagcca aaatattgga
    64381 agattataat atgccgcaaa tggtgatcga tatttgcaaa cagcatcatg gaaccacgct
    64441 aatgcgttat ttctacatca aagcgaaaga acgcaatccg gaagtaacag aagagcaatt
    64501 ccgttatcct ggaccaaaac cgcaaagtcg tgaagcaggg atcgtcaata ttgctgacag
    64561 ttgtgaggct gcagtacggg caatggatca tccgacgagc gaaaaaatcg aaagttttgt
    64621 ccataatctt atcgaagagc gtatttccga tggtcaatta gacgatagtg gactgacctt
    64681 gaaagaaatc cgaaaagtgg aaaaatcatt gatcagcggt ttaagtagca ccttccattc
    64741 tcggatcaaa tatcctaaaa tgaaaacaga agttgagaag ataaaggaag aacaagaaga
    64801 atagaaagaa gcgatgtata atggatatta cctttatgga tgaaacaaac caagtctcag
    64861 aagagaaaat gaaagaaatc gatgacttgc tgcaatttgc agcatcttat ttaaagctgc
    64921 cagaggaaac agaaatgtct gttactttca tggacaatgc ggcaatccaa gtaatcaatc
    64981 gtgattaccg tggaaaagat gcaccgacag acgtcatcag ttttgctctg gaagaagaag
    65041 gcgaagacga aatcccagtg atttttgaag atggagagaa tccattgcct cgtgtactag
    65101 gagacatcat gatttctatt gaacgtgctg ctgaacaagc agaagcttac ggccattcat
    65161 atgatcggga acttggcttt ttagcggtac atggattctt gcatatcaac gggtatgacc
    65221 atatgacacc agaagatgag aaagaaatgt ttggtttaca gaaagagatc ttggatgcct
    65281 atggactcaa aagataaaaa cgtagagaaa aacaaacatt tcctgatgtc tgtggaattt
    65341 gctgtccaag gagtcaaaac cgtttttgag gaagaacgca atatgaggaa acatgttgcg
    65401 ttcggtatcc ttgctttatt tatgggcgtg attttccagc ttgatcggat agagtggtta
    65461 tggctgcttt tagctgtttt tctcgtttgg atagtagaga tcatcaatac tgtttttgaa
    65521 aatgtagtgg acatgtttac ggattttcat tttcatccga ttgggaaaaa aatcaaagat
    65581 atggcagctg gagcagtttt agtgaccgct tgttttgctg tcatcattgg gttgatttta
    65641 tttgtcccta aaatatatca tttgttgttt aattaaaaag gagaacgtat atgccagaac
    65701 ataaatcagg gtttgtcgca atcgtcggcc gcccgaatgt aggaaaatcc acattattga
    65761 accgaatcgt gggtcaaaaa attgccatta tgagtgacaa ggcacagaca actagaaata
    65821 aaatccaagg agtatatact acaccagaag ctcagctgat ttttatcgat acaccaggaa
    65881 tccataaacc aaagcatcgt ctaggagatt ttatggtaga agcagcttac agtgcgttga
    65941 aagaagtgga tgcagtctta tttatgatca gtgctgatca aaaacgcggc cggggagacg
    66001 attttattat tgaacgtcta aaaaacgtgc attctccagt ttatctgatt atcaataaaa
    66061 tcgataagat ccatccagac gagttgctgg gaatcatcga agattattcg acgcagatgc
    66121 cttttgcaca agtgattccg atctctgcta cagaaggaaa caatgtcgaa cgcttaatgg
    66181 atgtattagt cagtgaaatg ccagagggcc cacaatattt tccagatgat caagtgactg
    66241 atcatcctga atactttatc gtttctgaat tgatccgtga gaaagtcttg tttttaacac
    66301 gagacgaagt gcctcattct gtcgcagtcg ttgttgattc gatgaagcga aacgagaatg
    66361 ataaaatcca tatccaggcc acgatcatcg tcgaaagaga tagccaaaaa ggaatcatta
    66421 tcggcaaagg cggaaaaatg ctgaagcaag taggaacgaa agcaagaaag gatatcgaaa
    66481 atcttttagg agacaaagta tttctagaac tttgggtcaa agtccaaaaa gactggcgag
    66541 acaaaaaagt ctatctgcaa gactttggct accgaaaaga cgaatactga tattctgaac
    66601 ttcttatagg ttaagtatag aaataaactg gataaaaatc cgcacgttga gaaaaacggc
    66661 ggaagatcga aacataaata atctggatcg gcagaatctg cttttccaga tttatttttt
    66721 aggagggaga atatgagcca gcttgcagaa tcgaaaggct tgattttatc tagccgtaat
    66781 tacaaagaaa aagataaatt agtcaaaatc tttacggaat cttctggcaa aatgatgttt
    66841 tatatcaaag gaatccatcg gcaaaatcag ccattagctc cggctttgct cccctttacc
    66901 gaagcagtct atatcggtaa ttttcgaagt gaaggtttgt cgtttttaag cagtgtcaaa
    66961 gacgtccatg cgtttcggaa catccaagaa gatatctttt tgagcggtta tggtacctat
    67021 ttgctaaatc tagtagatgc agcaatcgaa gatcatcaat atgacccgca tttattccaa
    67081 tttacgcaac aagctctgca gcggatggat caaggagatg atgcagaaat catcacgaat
    67141 atctttgaag ttcaaatact gcaacgcttc ggaatcgcgc caatttggac gcactgtgtc
    67201 gtatgcgggg aaacaaaagg aaaatttgac tattcttcca aatctggcgg tgtgatatgc
    67261 gagaaacact ggccaatgga cgaacaccgt tatcatgcag atcctcgcgc tgtttatttc
    67321 gttcgaatgt tttcggctat ttcttatgat aagatcagtg gaatccactt gaaagaagaa
    67381 acgaagcagg caatccgtga cctcattgac cagttgtatg atgaatatgt cggtattcat
    67441 ctaaaaagca aaaagttcat tgatcaaatg aagtcttggg cacacgtatt aaagacgcca
    67501 gagaaaaata tgcctgaaaa agagatacct gaaaaagaag aataaatgaa agatacaaca
    67561 aaaaaatcaa aactatttga caaatacagg gacttccgtc tattattaac cgtagattaa
    67621 atagatcgtt cactgagagg atctattata taaacaagaa aactgcaaaa tgaatacgta
    67681 cattgaccaa gatcagtaaa aaacacgtgg tttagcgagt tcgggagagt ggaagccgga
    67741 cccaaacgat ttttgaagag gcacttgcga gtacgataaa tgaagctgcc gaatctttat
    67801 tcggaagtag ggtggaaccg cgaaattatt cgtccctatg tctgtaatca gacataggga
    67861 ctttttgtcg ttttctttcc tgtattctta ttgacagcaa cgcttaaaaa ttgaaagggg
    67921 caaaacagat gagtaaaaaa ttgaccgtcc aagatatgat tttaacatta caaaaatttt
    67981 ggtcatcaaa cggctgcatg ctgatgcaag cttatgacac agaaaaagga gcaggtacga
    68041 tgagcccata tacattctta cgggcaatcg gaccggaacc atggaatgct gcttatgtag
    68101 agccatcccg ccgaccagct gacgggcgtt atggagaaaa cccaaaccgt ttgtatcagc
    68161 atcatcaatt tcaagtcgta atgaaacctt cacctgaaaa tatccaagag ctttatttag
    68221 aaagcttgcg tcttttagga attgatccat tagaacacga tattcgtttt gttgaagaca
    68281 actgggagaa cccatcgatg gggtgtgccg gtgtgggttg ggaagtatgg cttgatggga
    68341 tggagatcac acaattcacg tacttccagc aagtcggagg actaccttgt aaaccagtta
    68401 cttctgagat cacttatgga ctagaacgtc ttgcttcata tattcaagaa gttgaaagtg
    68461 tctatgatct ggaatgggct gatggtgtga aatatggcga gatcttcaaa caaccagaat
    68521 atgaacattc caaatattct tttgaagtaa gtgaccaaga cttgctacta agcaactttg
    68581 accgttttga aaaagaagct aaacgctgta tcgatgaaaa tcttgttcat ccagcgtatg
    68641 attatatttt gaaatgcagc catacattca atttattaga tgctcgcggt gcagtatccg
    68701 taacagaacg agcaggttat ctttcccgta tccgcaacat ggctcgtgct gtagccaaaa
    68761 tctttgtggc agaacgcgaa aaattaggat tccctttatt gaataaaaac acagcaaatc
    68821 cagaaaacat gaaggaggct gaataagatg gcaaaagact tattacttga aatcgggctg
    68881 gaagaaatgc ctgctcatgt tgtcacacca agcatgaaac aattagaaga aaaaacagca
    68941 aaatttttag atgaaaacca attgacttat gatacgatcg aaacgttttc tacaccgcgc
    69001 cgcttagcaa tcaaagtaac aaacattcca gaacgtcaag cagacagtga agaagaagtc
    69061 aaaggaccag ccaaaaaaat tgctttggac gcagatggaa actggacgaa agcagctgaa
    69121 ggatttgtcc gcggacaagg tctgacgaca gatgctatca cttttagaga attaaaaggc
    69181 gtggaatatg tctatgtaac gaaatacact cacggcaaag aagcgaaaga agtattgaca
    69241 ggtttagcag atgtcattaa aagcctcact ttcccagtaa cgatgcattg ggcagaatac
    69301 gattttgaat atatccgccc tattcactgg atcgtggcat tgttagatga cgaagtaatc
    69361 ccgtttgaga tcttagatgt caaaacaggc agagcctctc gaggacatcg tttcttaggc
    69421 gaagatgtga catttgcgca tgcaaaagaa tatgaagaaa aattgaatga acaattcgtt
    69481 attgcttcac cgcaaaaaag acaagaaatg atcgttacac aagcaaatga attagccgaa
    69541 aaaaatcact gggttattga tctagacgaa gatttactag aagaagtcaa caatttagtc
    69601 gaatatccaa ctgtcttttc tggcgacttc gaagaaaaat atctttctgt tcctgaagaa
    69661 gtactagtca cttctatgaa agaacatcaa cgttatttcg aggtacggaa cgatcaaggt
    69721 atgctgcttc ctcattttat ttctgctcgt aatggtgatc gggtaaaatt ggaaaatgtg
    69781 gttcgaggca atcaaaaagt attgacggct cgtttagaag atgcggaatt cttttataat
    69841 gaagacaaga aagcttcaat cgaccaatac gttgaaaaac taaaaactgt tactttccat
    69901 gaaaaaatcg gtagtatcta tgagaaaatg cagcgggtac aagtcattgc ccgtatcatt
    69961 ggcgaacaag taggactatc tgaggaagaa ttgatggatc tgcaacgagc tgctgaaatc
    70021 tataaatttg atctagtaac caacatggta ggggaatttc ctgaactaca agggatcatg
    70081 ggcgaaaaat atgctctgct gaaaggcgag aaacctgctg tagcaacagc gattcgtgag
    70141 cattacatgc ctatatccag tgaaggagag ctacctcagt ctacagtagg ttctgtttta
    70201 gcgattgcag ataaattaga tagcattttt tctttctttg cagttgggat gattccaagc
    70261 gggtcaaacg atccttatgc cttacgtcgc caagcatacg gtgtcgttcg gatcatcgaa
    70321 tcgaaaaaat gggcctttcc tttgtctgtt ctgcaagaaa cgattagtga tgttatcagt
    70381 aaagatacgg atcgtttcgg cattggttta tcagaagggc aacaacaggt gatcgacttt
    70441 atcaaaggcc gtctgcgcca attattgaca acgaagaata ttcgtcatga tgtcatcgaa
    70501 gctgttttga atgcagaaca aaaagatctg acaaaagtat ttgcagcagc acaattgttc
    70561 aagcagcatc ttgcagatga agacttcaaa ccatcgatgg aagctttgac acgtgtcgtg
    70621 aacttagcga aaaaagcgga gcttgaaaaa caatcagaag tagaccctga actgttcgaa
    70681 aatgaagcgg agaaagaact gcacaaagca gtagaggcaa tgaaaaaaag ctttgctgac
    70741 caaacgatca acgaaaatta tgatcagctt gtaagccttc gcccactgat cgaaaattat
    70801 tttgatcaga cgatggtcat ggcagaagaa ccagcagtac gtaagaaccg attaacgcaa
    70861 ttaagtcaaa ttgctgccat ggctttgtcg ctagcaagtt tagatcaaat catcacaaaa
    70921 taatttctaa tatgtaaata aaccagagaa taggcacaaa a
//
LOCUS       DACDTH010000007        68684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDTH010000007 DACDTH010000000
VERSION     DACDTH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.007"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(592..1983)
                     /locus_tag="IWK54_000525"
     CDS             complement(592..1983)
                     /locus_tag="IWK54_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9592673.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2196..2582)
                     /locus_tag="IWK54_000526"
     CDS             complement(2196..2582)
                     /locus_tag="IWK54_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9592674.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2638..3261)
                     /locus_tag="IWK54_000527"
     CDS             complement(2638..3261)
                     /locus_tag="IWK54_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9592675.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3325..3993)
                     /locus_tag="IWK54_000528"
     CDS             complement(3325..3993)
                     /locus_tag="IWK54_000528"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9592676.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4005..4445)
                     /locus_tag="IWK54_000529"
     CDS             complement(4005..4445)
                     /locus_tag="IWK54_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592677.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4504..5176)
                     /locus_tag="IWK54_000530"
                     /pseudo
     CDS             complement(4504..5176)
                     /locus_tag="IWK54_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5361..5759)
                     /locus_tag="IWK54_000531"
     CDS             complement(5361..5759)
                     /locus_tag="IWK54_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9592678.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5756..6529)
                     /locus_tag="IWK54_000532"
     CDS             complement(5756..6529)
                     /locus_tag="IWK54_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9592679.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6526..7209)
                     /locus_tag="IWK54_000533"
     CDS             complement(6526..7209)
                     /locus_tag="IWK54_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9592680.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7376..8263)
                     /locus_tag="IWK54_000534"
     CDS             complement(7376..8263)
                     /locus_tag="IWK54_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9592681.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(8260..9129)
                     /locus_tag="IWK54_000535"
     CDS             complement(8260..9129)
                     /locus_tag="IWK54_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9592682.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(9266..10552)
                     /locus_tag="IWK54_000536"
     CDS             complement(9266..10552)
                     /locus_tag="IWK54_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9592683.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10825..11403
                     /locus_tag="IWK54_000537"
     CDS             10825..11403
                     /locus_tag="IWK54_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9592684.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11403..12332
                     /locus_tag="IWK54_000538"
     CDS             11403..12332
                     /locus_tag="IWK54_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9592685.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            12329..14065
                     /locus_tag="IWK54_000539"
     CDS             12329..14065
                     /locus_tag="IWK54_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9592686.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(14128..14964)
                     /gene="pheA"
                     /locus_tag="IWK54_000540"
     CDS             complement(14128..14964)
                     /gene="pheA"
                     /locus_tag="IWK54_000540"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9592687.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14961..15470)
                     /locus_tag="IWK54_000541"
     CDS             complement(14961..15470)
                     /locus_tag="IWK54_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9592688.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15463..16758)
                     /gene="aroA"
                     /locus_tag="IWK54_000542"
     CDS             complement(15463..16758)
                     /gene="aroA"
                     /locus_tag="IWK54_000542"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9592689.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16771..17862)
                     /locus_tag="IWK54_000543"
     CDS             complement(16771..17862)
                     /locus_tag="IWK54_000543"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9592690.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17879..19045)
                     /gene="aroC"
                     /locus_tag="IWK54_000544"
     CDS             complement(17879..19045)
                     /gene="aroC"
                     /locus_tag="IWK54_000544"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9592691.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(19047..20111)
                     /gene="aroB"
                     /locus_tag="IWK54_000545"
     CDS             complement(19047..20111)
                     /gene="aroB"
                     /locus_tag="IWK54_000545"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9592692.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(20144..21166)
                     /gene="aroF"
                     /locus_tag="IWK54_000546"
     CDS             complement(20144..21166)
                     /gene="aroF"
                     /locus_tag="IWK54_000546"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9592693.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21825..22799)
                     /locus_tag="IWK54_000547"
     CDS             complement(21825..22799)
                     /locus_tag="IWK54_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9592694.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22780..24663)
                     /locus_tag="IWK54_000548"
     CDS             complement(22780..24663)
                     /locus_tag="IWK54_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9592695.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24681..26906)
                     /locus_tag="IWK54_000549"
     CDS             complement(24681..26906)
                     /locus_tag="IWK54_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9592696.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            27021..28055
                     /locus_tag="IWK54_000550"
     CDS             27021..28055
                     /locus_tag="IWK54_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9592697.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(28122..29135)
                     /locus_tag="IWK54_000551"
     CDS             complement(28122..29135)
                     /locus_tag="IWK54_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9592698.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(29213..29401)
                     /locus_tag="IWK54_000552"
     CDS             complement(29213..29401)
                     /locus_tag="IWK54_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9592699.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29841..29969)
                     /locus_tag="IWK54_000553"
     CDS             complement(29841..29969)
                     /locus_tag="IWK54_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9592700.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29972..31345)
                     /gene="rlmD"
                     /locus_tag="IWK54_000554"
     CDS             complement(29972..31345)
                     /gene="rlmD"
                     /locus_tag="IWK54_000554"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9592701.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31456..32511)
                     /locus_tag="IWK54_000555"
     CDS             complement(31456..32511)
                     /locus_tag="IWK54_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9592702.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32521..33951)
                     /gene="gatB"
                     /locus_tag="IWK54_000556"
     CDS             complement(32521..33951)
                     /gene="gatB"
                     /locus_tag="IWK54_000556"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9592703.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33948..35420)
                     /gene="gatA"
                     /locus_tag="IWK54_000557"
     CDS             complement(33948..35420)
                     /gene="gatA"
                     /locus_tag="IWK54_000557"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9592704.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(35420..35725)
                     /gene="gatC"
                     /locus_tag="IWK54_000558"
     CDS             complement(35420..35725)
                     /gene="gatC"
                     /locus_tag="IWK54_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9592705.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35831..37867)
                     /gene="ligA"
                     /locus_tag="IWK54_000559"
     CDS             complement(35831..37867)
                     /gene="ligA"
                     /locus_tag="IWK54_000559"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9592706.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37884..40118)
                     /gene="pcrA"
                     /locus_tag="IWK54_000560"
     CDS             complement(37884..40118)
                     /gene="pcrA"
                     /locus_tag="IWK54_000560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9592707.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            40273..41832
                     /locus_tag="IWK54_000561"
     CDS             40273..41832
                     /locus_tag="IWK54_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9592708.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41943..43874)
                     /locus_tag="IWK54_000562"
     CDS             complement(41943..43874)
                     /locus_tag="IWK54_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9592709.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43892..44806)
                     /gene="pfkB"
                     /locus_tag="IWK54_000563"
     CDS             complement(43892..44806)
                     /gene="pfkB"
                     /locus_tag="IWK54_000563"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9592710.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44803..45555)
                     /locus_tag="IWK54_000564"
     CDS             complement(44803..45555)
                     /locus_tag="IWK54_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9592711.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45714..45923)
                     /locus_tag="IWK54_000565"
     CDS             complement(45714..45923)
                     /locus_tag="IWK54_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592712.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45934..47262)
                     /locus_tag="IWK54_000566"
     CDS             complement(45934..47262)
                     /locus_tag="IWK54_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592713.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(47627..48214)
                     /locus_tag="IWK54_000567"
     CDS             complement(47627..48214)
                     /locus_tag="IWK54_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9592714.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(48297..49547)
                     /gene="clpX"
                     /locus_tag="IWK54_000568"
     CDS             complement(48297..49547)
                     /gene="clpX"
                     /locus_tag="IWK54_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9592715.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49794..51083)
                     /locus_tag="IWK54_000569"
     CDS             complement(49794..51083)
                     /locus_tag="IWK54_000569"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9592716.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(51237..52169)
                     /locus_tag="IWK54_000570"
     CDS             complement(51237..52169)
                     /locus_tag="IWK54_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592717.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(52326..52985)
                     /locus_tag="IWK54_000571"
     CDS             complement(52326..52985)
                     /locus_tag="IWK54_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9592718.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52988..54013)
                     /gene="sppA"
                     /locus_tag="IWK54_000572"
     CDS             complement(52988..54013)
                     /gene="sppA"
                     /locus_tag="IWK54_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9592719.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(54026..54316)
                     /locus_tag="IWK54_000573"
     CDS             complement(54026..54316)
                     /locus_tag="IWK54_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592720.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54725..56530
                     /gene="glmS"
                     /locus_tag="IWK54_000574"
     CDS             54725..56530
                     /gene="glmS"
                     /locus_tag="IWK54_000574"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9592721.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(56602..57957)
                     /locus_tag="IWK54_000575"
     CDS             complement(56602..57957)
                     /locus_tag="IWK54_000575"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9592722.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(57981..59150)
                     /locus_tag="IWK54_000576"
     CDS             complement(57981..59150)
                     /locus_tag="IWK54_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592723.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(59147..60034)
                     /locus_tag="IWK54_000577"
     CDS             complement(59147..60034)
                     /locus_tag="IWK54_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9592724.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(60223..63915)
                     /gene="nifJ"
                     /locus_tag="IWK54_000578"
     CDS             complement(60223..63915)
                     /gene="nifJ"
                     /locus_tag="IWK54_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9592725.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            64114..64635
                     /locus_tag="IWK54_000579"
     CDS             64114..64635
                     /locus_tag="IWK54_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9592726.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(65334..65891)
                     /locus_tag="IWK54_000580"
     CDS             complement(65334..65891)
                     /locus_tag="IWK54_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592727.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(66070..66681)
                     /locus_tag="IWK54_000581"
     CDS             complement(66070..66681)
                     /locus_tag="IWK54_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592728.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(66794..67051)
                     /locus_tag="IWK54_000582"
     CDS             complement(66794..67051)
                     /locus_tag="IWK54_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592729.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(67648..67926)
                     /locus_tag="IWK54_000583"
     CDS             complement(67648..67926)
                     /locus_tag="IWK54_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592730.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(67960..68211)
                     /locus_tag="IWK54_000584"
     CDS             complement(67960..68211)
                     /locus_tag="IWK54_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592731.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        19210 a        14833 c        12080 g        22561 t
ORIGIN      
        1 ctgttctaac attctcaact ttgtcagttt accataggaa aatgggaaaa tctacgaaag
       61 ctctcaacag aatcgtccta cttaaatacg cttttcttta ataataaata agttggttgt
      121 ttttgttatt tacgctttca cttttcggaa taaataatgt gtatttatat ggaataaata
      181 aaaaaagagg tcgtgactaa aaacaagtca caacctctgg taaacggtgt tctcctgaca
      241 actcctcggt cacaagacac aatattcaac aaacatgaga agctattttt tgaaattgct
      301 ctttgtgact cagagtgaaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag
      361 cttgcatctg cggtaataag ctcaaccaaa cgtaaaatat gaggaactat ttttgtttgg
      421 ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttattcac ggtgtttcag
      481 aagtagcttc tcggaaataa gccgaaattt cacaaaaatt tgagaaacta tttttaggaa
      541 acttcttctt atttcttaaa gctgaccact tctatcacaa cctctttttg actatgcatt
      601 cacatcgatc actttagaat catcattgat gacatcgcga tctaaatctt tgatgcgtgc
      661 acttgtcacg ataccagcta ccatgctacc gctgacgttt actgctgtac gtgccatatc
      721 gattaatggt tcaacagaga taactaaccc aacgattgct actggcaagt tcattgcccc
      781 taaaacgatc agtgaagcaa atgttgcgcc cccaccgact ccagctacac caaaagaact
      841 gattgttaca attgccacaa gcatcaaaat aaattccaaa ctaaagacat tcactccagc
      901 tgctggtgcg acgatcgttg ccaacatagc tggataaacg ccagcacagc cgttttgtcc
      961 aatagacaaa ccaaaacttg ctgagaaatt agctgttgca tcatctactc ctaaagcttt
     1021 tgtctgagtc tcgatattca atggcaaagc tccagcactt gaacgtgaag taaacgcaaa
     1081 gctcaatacc ggaaaggctt ttttgaaata gttgattgga ttgacttttg caccaatcaa
     1141 aatcagtgag tggacaatca aaacagcaag caatgctacg taagaagcaa cgacaaattt
     1201 acctaaattg acgatagctg caaaatcact tgttgccaaa acgtttgtca tcaaagcaaa
     1261 tactccataa ggtgtcaaac gcaaaacaag cgtcacgatc cgcataatga ttttatacag
     1321 actgtcgact aaatctgaaa agaatgctgc ttcttttggc gcttttcttt tcacgcctag
     1381 ataagcgatt ccgacaaatg ccgcgaaaat aacgactcca atcgtacttg ttgcacgact
     1441 acctgctaaa tcagcaaaaa cgtttttagg gataaaggat aaaatctgct gtggaatcga
     1501 taagttttca actgtttcct gacgtgttgc cagttcagag attcgtgctg tttctgcagc
     1561 tccttgtgtg aatttagctc cttggagtcc gaacagcatt gttgaaccaa ttccgatcaa
     1621 tgctgcaata gctgttgttc caagtaaaga agtcaataca gtcgcactaa ttttccctag
     1681 cttttcagaa actttcattt ttgtgaatgc accaatgatc gaaataaaca ctaaaggcat
     1741 aactagcatt tgcaagaatg atacatatcc ttgacctaca atcccgatcc attccattga
     1801 ttgagtaacg actttgtcgt ctacaccaaa gatcaattga agcagaccgc caaagacgat
     1861 accaactcct aatgctgtaa agacgcgtgt tgaaaatttt gtatggcgct tttgcatacg
     1921 atagaacaca tataataatg ccgcaaaaac tagaaccact aaaactgtga tgagtgttgt
     1981 catttttctc ctcctcatat tattaatgac aagtccatcg aaggaagtaa aggttcccga
     2041 gaatgattct tcaaccatcc tcctgaaaaa aattttttca gtgactttta tattttagac
     2101 gatttttatc gaaatgacat tctttttgct tacaattagt aataaaaaaa catacctgtg
     2161 atagctttaa tctatcgcag gtatgttttt actgtctatt cttgtttttc tgattgaaaa
     2221 ttcaatttta ccccaggctg atctaaggcg atctctgtca cctcataaga cattcggcgt
     2281 acgatatcga ataacggtgc tactagctcg tccacttctt cttgtgtgac atcattttga
     2341 ccttcaattt tccgattgag gatatgattg atctgactca cacttccaga taaaacatat
     2401 gtatcaaaca ctaaacggaa ctctaagcgt gctcctacga tgctatgttc tttcggatag
     2461 ttttcgatcg tttcttttaa aggcgagaag cctactttca aatctgtttc tacttcttgg
     2521 ttcggcaatc tcatatcata atgaaatgct tctacaactt ctttttgtct tctgatttcc
     2581 atataatttt atcactcctt tatgaaaatt ataacatatc tcctttgaaa ggggatacta
     2641 ctttttaatg atgctctgca aggtaaggat ttcattcaga tttcctacca cataatctgc
     2701 cgggatctca cccagaaaat gatcgatgtc gtaaagacaa gtagcgactc ctgcattttt
     2761 ccctgcttcg atatctaaac gacgatcgcc gatcatcatc gtgtctgttc tttctaaatg
     2821 aaaagtatcg atcaaataat ttaaagatgc tggatcaggt ttacgaggaa agtcctgatc
     2881 gatccctact acttcctcaa tcagatgtgc caatcgatgt tcttttaaca ggccccatgt
     2941 tgattctgtc aaacggtggg tcaggatgaa atgacgaccg ccgttgtctt ttagcatttc
     3001 cagtacttgt ttcgtttctg gaaatggacg ggaaacttcc gtactttctt tttcatatcg
     3061 gtgaaataat tcattaaact ctctggcatt caagtttgat tcattgatca aataactagt
     3121 agaatacttt ttcattttga agtagatttc ttttttgtcc atactgatac caaaatcttt
     3181 tagtgcttgc catgcaccat ctaccattgc gggatacgta tcaaacaatg taccatcaaa
     3241 gtcccatata taatttttca tgttcctttt gttccattcc tttgcttctt ttttctatct
     3301 ctcactttta gataaaggtg ttctttaaag tatttttttg tccagaattg tcaaatcatc
     3361 tgctaatgta tactggattg gttgtgcatt tgggatatcg atcgtatcca tttgatcttc
     3421 aggaacatct tctaaatatt ttgtcagtgc tcggatgcta ttaccatgac ccacaactag
     3481 taaatcttgc cgatctttta gttttggtgc tagatgatct tgccataagg gaatgatccg
     3541 attcactgtc atttccagac tttctccgta tgggatcaaa tgcggatcca agtgatcata
     3601 tctgcggtca aatctatttt ctgcggttaa cggcggaact tccgtatatc ctctgcgcca
     3661 tgtttttact tgatcttttc cgtatatttc tttcatctta tctttgtttt tacccacaag
     3721 atccccataa tgccgttcat ttagacgcca tgtttttatt ataggaagcc ataactgacc
     3781 catttcttct agtgcgactt gtccggtaag aatcgcacgt ttcaaaacag aggtataaac
     3841 ggcatcgaat tgaagtccgg cttcaagcat tttttgaccg gcaactgctg cttgttcttt
     3901 tcctttttct gttaaggcga catctagcca tcctgtccag taattttcaa aatttgcttc
     3961 gctttcgcca tgacgtaata atactaattt cattcttttc tcccttaaac attcgtttcg
     4021 aattttatac cacatgtttt gcatgtcaca cgtattcgtc cttttccttt aggtgcacgc
     4081 tgcttttgat gacaattcgg gcactcaaaa aaagtataac tttttcgatc cattttgact
     4141 tgctttttgt tttttatctg atgatatttt tctttgactc tatcaagata tttcaaataa
     4201 cgttgattct catttagtct ggggtagatt ttttttgata ggaaacgaaa tatcatccat
     4261 ccgaaaaaga cgatgaacaa caattgtccg attctatagg gcaaccaacg acttagcaaa
     4321 ctaaagacaa gcgcagcgat caatagaccg cgattcaact gatcaaattt cgcatatctt
     4381 ccctgcataa gtttgtgata gttctgctga aatcgtatta gcctttctaa ccatgtttgc
     4441 atcatattat ctcctccatc atctaatatt tattatgaca aaaaaatcca aaaagaaaaa
     4501 gccttaattg gccttttcca ttgcaacttg gtgaaggaca ttccgatttc tggtaaagtc
     4561 aatcacaatc actttctttt tatcttctct gttgactact cgaaatgtgt ccgtcaatgg
     4621 aagaaacagc ttcttagatg gtagctgttg catacgtata aaacgatgga tcatcgtaat
     4681 caattgagga aattgaatag acgtgtgaag cagacctgga tattttttca acagctgtgt
     4741 cgttttgatc agattccgtt tttttactga tcacttttaa aaagcttttt agaatctttt
     4801 gctgacgtct aaaaatagaa aaagaatttt cgtcatctat ttgataacgc atttgttttt
     4861 gcagttctgc tgcagtcaga tggatcttcc catcgtttgt atcgatcatt aaaccttctg
     4921 gaaacagttc aaccactgct cgatcccaag attcaagaga aagctcgata taatctttta
     4981 ctggtaattc caattctacc ttaaaaaagt gcaaaacagc cgacagccct tctgcttgat
     5041 agatttctcc taatgttagc ccctctagat ggaactctgg tgaaaaatgc agcaatgttt
     5101 tattttcctg caattgaaca tatagaaatg tagatcgctc aatcagagta tcttttaaaa
     5161 caactaaaaa attcattttt ctcccccttt tttcatcatt tttaaaatag catattttga
     5221 aaagaaagtc tgatgcttag agtagaattt cataaaaatt tactatttca taagtattca
     5281 cgagaaaaat acaaacaaaa aagcggaaat aacgctcatc cgccttcttg tttgtgttta
     5341 tttcctcttc aaacgcttgt ctatgattca gacttttctc tatcgttatt ttcatctaaa
     5401 agttcttgca ttccgataat ttctcgatat tgatgaatct tagtgatacc aatgactttg
     5461 tctacatcaa tactaaaaca aataccatta cctggaatat cgatgttgat gccttgacca
     5521 atcgcatcca ttactttatt cgtcaaacta tcgggtacta atgtcaacac aatatctttt
     5581 tctggcgaaa tgtccaaacc taaaaatttc cctgaatcat gtacaccaga acctcttcca
     5641 tgtaagatcg ttccgcctgc tgcacctgct tcttttgaaa agtcgataac atcttcgccc
     5701 attcctccgt caacgatcgt cacaatcatg tgaagattca atgctacaag atctttcatt
     5761 tgctttcccc caatttttgc aatctttctt tttcgattct gtctttttca tcttttcgtt
     5821 tataaatata tcctagtact aaagtggata atattggtgt caatgcaatc attgcaacca
     5881 caccaaaccc gtcaatgatc ggatctcttc cttcaatttg tgcagcgatt gccacgctca
     5941 ttgataaaat aaatgtcgaa cacatcgggc ccgttgctac tccaccgtta tcaaaagcca
     6001 ttgctaaaaa gatcttgtcc acttttcgtc ctaaaataaa tgctagcccg tatcctggga
     6061 tcaagaaata ataaagagag aatccgacga gcaaacgcca catagacaat cctactgctg
     6121 cgccgatccc aattgaaatt accatcagca tgattttcga tcggattctc ccctcgctca
     6181 attcttcaac ctgtttgatc attacatgaa tcgccggctc agcaaaccct actaagaacc
     6241 ccattaaaaa gccaagggga accagtatat aattgtaagg caatcccgca atagcactgc
     6301 ctaaatgctg tcctaccggt acataagcca tattgactcc gtgcaggaaa aggatcaacc
     6361 caatagtcgt tatcaagaaa cctttcataa tgtcaaagaa acgctttttg cgcatacgaa
     6421 aactaatgat attcattaaa acaaatagga ttacgatagg tgccactgcc aaaatgactt
     6481 ccatggtaac gatttcgatt ccatctatca cttgctgcca gaaactcatt ggaagatcac
     6541 ccccaaaatc atcacggcaa gaatcggccc taacgaagca ataccgacca ttccaaaact
     6601 gtcattttca tttttctttt gtttgatcat actagtcatc cctccggcaa gggccaagat
     6661 aaatggaaca gttactggac cagtagtcac tccccccgaa tcaaaagcaa tcggcataaa
     6721 ttcggaactg gtgaaaaatg aggcgatcaa aacgcctatg tagcctatag ccatcaattg
     6781 ataataggat agcttgaata cgacccgaag cagcgcaaag gctagaaata ctccagtgcc
     6841 gacgctgacg atcccaatca gcaacactct gccaatctct ccgtcagata tatcatatac
     6901 ttgttgtccc aaaacttgca cggaaggttc agcaatcgtg atcacgatcc cgatagcaaa
     6961 tcccaaactg accaaaacgg tcaaactttt ctttttgatc atatatttcc ctactagatc
     7021 tccgacttcc atcatagaat aatctgctcc aaacaaaaag agcgtcattc cgatcatcat
     7081 gattgccgca ccaaccagaa atgaagcgag gtcttctcct tctaacggtg caaaaacaaa
     7141 cgttaaaatg acaatcagga cagtcatcgg caatatagca gcaatcactt ctttaaaatt
     7201 ttttagcatg tatcctcctc ctttactctt taagtttacc ttaaaatgaa agagttttcg
     7261 agcacaaact taaaacgtta tcatttaatt tttctcaaca taaaataaca cacgtcccaa
     7321 aagtatcttt ttacttggaa aaatgacttt gaaggaacgc gcttattgtg tttttttatt
     7381 ttacagagcc gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat acgatggcag
     7441 ttggtaaagt agcgatgact aatccagcca tgatcattcc atagtctggt gtataggaag
     7501 cacccatagc agataaaaca agcggaaccg tccgtttatc aggagattgc aaagctacta
     7561 agggccataa ataattgttc cagctactca tgaatgtgat gattgcagcc gcggcatagg
     7621 tatttttagc cgtcggcata tacactctaa aaaaaatact tagttctttt agaccgtcta
     7681 atcgagcggc ttctactaga tcttttggaa aagatttaac attttgtcga aagaagaaga
     7741 tcaaaaaagc agtgctaaca gatggcagga tgacaaccaa gaaactgttg attcccaaaa
     7801 ccgttccgtt caattgtgaa aacattcgat ataaagggat catcaatgcc gcaaaaggaa
     7861 ccatcatcga tagcagtaga atattgaaaa ttaggtcttt tcgtttactt tgataaatct
     7921 caaatccata tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc accaaagcga
     7981 tcatcgctga attccaaagg gaacgagtaa aattcagctc attggaaaac aaattttgaa
     8041 aattgatgat caagttactg ccaatcttta tttttcccga tgtgatatcc acgggtgtat
     8101 tcgtcattcc caaaaccatc cagacaaaag ggaaaatgga aataaatgat aggatgctta
     8161 atgctgtata ctttaatact gacccaattg tcgaacgaag ttctaatgct tgttttttga
     8221 cgttggggcg ttcgattgtt tctttttcta ccatattttt catcgacttt ctcctcctac
     8281 tttcgtttgg attatcgaca atatcacaat aaaggaaaca ataacaaagg aaactgctgc
     8341 cgcatatcca aaattaggcg tgaatttata actcaaatta tagatgtatt gagatagggt
     8401 ggtcgttgca tttgctggtc ccccgccagt aatattttgt acttcatcaa aaagctgcaa
     8461 tgtcccgatc gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat ttgggatcgt
     8521 cactttgata aactgctgcc atttagaagc accgtcaata gatgctgctt cataaatctc
     8581 cgaatcaata ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag tccaacgcca
     8641 agtgatcgaa agaatgatca gaactctcgc ccaaattgga tgagtcaacc agccaatcgg
     8701 tgtgtcaatc ataccaatgc ccaacaacaa gttgtttaca aggccagatt gagaaaatag
     8761 acttttcatg atcagagaat aactgaccat cgaagtgata catggaagaa agatagcggt
     8821 tcgaaacaac cctttatatt tcaacttttt atcatttaaa aggttggaaa taaaaagtgc
     8881 tagaaacaac atgatcggta cttgtacaag taaatacagg aatgtattaa aaaaagcttt
     8941 tttaaaagtt gcatctgtca acatccgctg ataattacct aacccgttaa atgtcatatt
     9001 gacaggggga ccactttgaa aagacgtgat caaagccaaa accatcggaa taaaaaccat
     9061 gattgatatc aaaatgactg gcaagattaa aaaagcatgc ccgtttttga ataaatcttt
     9121 ctgcttcatt cttttccctc caattctatc attgattatc tacatgaaaa gacaagaaag
     9181 acaatatctg taaggcagac gtgatctttt ttgtcttttc gtttgtctga tgaaaaagcg
     9241 aagaaattca cttttttacc accgatcaat tagccaattg tgcttctact tgtttttgtg
     9301 tatctgctag taccttgtct gtctcttctc cattgatgat tcgatgaaga gcttcagcta
     9361 cgactgcttc aattgcatag gtctcatgtc cgtaattcac ttctggtatt tcagatgtcc
     9421 atgttgaaaa atcgtcaaag atcttttgac cactgtaaaa ttcagaagct tcttgataag
     9481 cttcttgttc attcgcgcta agcatcgtag atactagtcc gatttctttc gcaagagtgc
     9541 ccatcagttc ttcattcgaa gcaaacgttg cttctaggaa atctttcgct gaatcttctc
     9601 cagctactcc tttgatcaca taccagccag cgccgccaag gttagaagct tgcgcttttt
     9661 gcagattttc tggaagtgca ggtattggtg caatcttcca ttttccagat tggttttctg
     9721 ctccttggat cgttgaagaa taccaagcgc cagttgggct agaagctact gctcctgatt
     9781 gtatcgcagt tactccagca ttccagtcag aggtttgttc gactagatct tctttcaaaa
     9841 gagtggcaaa caattcaaga ccatacttta aagatgcatt gtcttggatc gtgactgtct
     9901 ccccatcttc agatgtatac cattctcctg cctgttgcat aatcatcctt actcgaccaa
     9961 ggtcagaagg attgacttca gcgattttct ttcccgtctt ttctttgaca tctcgcgcta
    10021 cttgaatata ctcgtcccac ttcagattct ccatatcctc ttctgtataa cctgcttctg
    10081 ccatcagatc cgtacgatag aagtttgctg ttacgcccga atcgaaagga acaccgtata
    10141 ttttatctcc tactttgttt accgcaaatt tatacggtgc aaaatgatct tcctttacaa
    10201 tagcagacaa attagcgaat gcatctggat aagaagtcaa atagccttgt atcctataat
    10261 cttcaatcaa gacgatattg ggcaatcctt ctgtattccc gctcgctaaa gcggtgttca
    10321 gtttctgaac gatatcatcc tgagacatcg taacaacttc tatctctgtt tcttcgttct
    10381 cataaacttt ttttgcttca ttcactgctt tgatattgaa tgtctcatcc cacgcccaga
    10441 cagtgacttt ttcttctttg tctgctgtac tactagcact tccgcatcct gttaagaaac
    10501 ctgatgctgc cgctaaacaa acgactccag caattaactt tctgatgttc atttttatcc
    10561 gctccttttc catccattag ttgtttacgt ttgctacatt catcatacca aaatttgaaa
    10621 acggtttctt tattattcag atacttcaaa aatttctcct aaaataataa aaaaatacaa
    10681 atcaactatg acaaaaggaa taatcttagc atctagacca aagatcgaaa tggtgaggtt
    10741 tctttaaaat aaaaatatcc ttgcattttc tcctagaaat aagtattttt aaattacaag
    10801 actattctta aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga tgatgaacag
    10861 acgattttag atggaatgaa acagttgatc aattggtctg agcacgggtt tcaattaata
    10921 gggacttttt ccagtccgct gaaagcgctt gatttttctt tggtacatgc aatcgatatc
    10981 gtggtcacag atgtaatgat gcctgaatta aatggaatcg aattatcgcg gctattaaaa
    11041 cagcaacaac ctgaaacaca gatccttatc ctctctagct atgatgaatt tgatatggtt
    11101 aaagattctt ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt gagtcctgat
    11161 ttttttctaa attccttaac tgccgtttca caaaaaatca aaaaaaagcc caacaaaagc
    11221 ccaatcacca atcgttatga gcagatctgc gagaatcttt cacagcagat tgctggacaa
    11281 actcgtccat tgcttgaccc tcttcttttt gaacacacct tgtttttctg ttatataccg
    11341 aaaaacggcc attcagttca aaacaaggct ctaaacaaaa acagtcgatc acccattcat
    11401 tgatggatag cgacgattcg tttactattt atcctgtttc taccttttcg gaagaatccg
    11461 gctatgtaat caatactgat tccaaagata cacttaggaa gtttgttcaa aagtttcgtc
    11521 aagaagtttt tccaggtgat ttttttgttg tttctgattc ttttgcaatc caagatatca
    11581 atgaaatatt ccaagagatc agaaattgca ccaaaggaca acgtttctat cataagggaa
    11641 agaaccttgc ttatcagcat gaacttcttc cgttattaac caaccacgag cagcaagcaa
    11701 gtagttattt aacaaaaatc gttcataaag actacgcttt agctgtcaaa gaacttgaga
    11761 atttcttcac gacgatattg gaacagccta ttgatccagc ttatttgaaa catgaggcaa
    11821 tcaatcgttt ctatgcatta ctcaacgctt tggctgacga gcatgttggg aatgaagaac
    11881 tttctttgct caagatcgcc cttccttctt tattagccga tgctgagtat ttcgaagatt
    11941 tttctattct tgtcaaagaa acacttgaga aactcaccca cttttctgca aatgcaaaaa
    12001 aagacaattt cgatatcttg actttgatct acgagttcgt ccaggaaaat tatgagaaag
    12061 aaatcaattt acaactttta tccgaaaaat atcatttttc atatagctat ctctcagcca
    12121 tctttactga aaaatttggt atcaactttt caaaatatct aaaaaaagtc cgtatcagta
    12181 aagccaagct ctttttgacg gaaacacggc ttactttgac ggaaatctgt gagaaagtcg
    12241 gctataccga actgggttat ttctccagag tttttaaaga agagacagga atgacacctt
    12301 ctcaataccg taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc atgaactctt
    12361 tgccaaactt ctgttcattt tgttcatcgg tctttttctg caagccatcg tcatttcttt
    12421 gtttatttat catcgatcaa gggatgccta tatccagctg ttcaatcaat cgaatgatgt
    12481 ggtactaaaa aagattcaat ctgaatttga aagtttgaat gatacaatcg agaatacatt
    12541 agccgctttt gattctaatc cagctgtcaa aagctatttt tccaatgatc cagcccaaca
    12601 tatggaacag cttcagcaac taagaacaat ccaaaaaatg aatgactctc tttcaaaaat
    12661 ccatccaatg atcgattacg atgtattgat ctttggagaa aatggaagaa cttttgtcgg
    12721 aaacgacatg ctcactgccg tatctgccga ttcttttttt caatctgcga ttgctcagag
    12781 agtgaatgaa cgtgctgctg atacacaaat gctttttgca caccacggat tgactcttcg
    12841 cgataagaaa gcacccagcg tcttctttgt caaaaaactg aaaaatacat tgaatcacgt
    12901 ttacggatat gctgttttat cgatctcatc caagcaacta gcgaatcttt ttcaatctgt
    12961 agtaaatcct gagatatcga agatctcctt catgaacgaa gaacagacga tcatcgcatc
    13021 aaatgaagaa aatacaattg ggagccgatc aatgattttc aaaaaactaa cagtaggtga
    13081 aacaacgatt tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc aggattgtac
    13141 attactcagt caagtcgatc ttcattcttt atctaaccaa atgggcgtga tttttccgat
    13201 tattttgtat aatatcgtga cacttgcttt tgctggctca ttggtattta tgtatttgga
    13261 tcgacacaca aaatcgatct accgcttgat tgattcgcta aaacggctag aggaagatgg
    13321 cttaaaagct actgttcctg tacaaggaag ccatgaagtc caattgctag ggaatacgat
    13381 caatcaattg ctaagtaatg ctcagcatca ttatgaacag acgatccaag acgaaaagaa
    13441 gaaacgttcg cttgaaatcc aaactatgca agcacagata cagccccatt ttatttataa
    13501 tacgctgact agcttgaaat ttttgatttg gcagaaaaaa acagatgaag cagttcaagg
    13561 aatcgacagt ttcattactt tactaagaag tacgattggg aaaaaagaag aagtgattcc
    13621 agtcaaggaa gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta tgcgatatgg
    13681 cgaaagaatc tcggctaaaa taatgattcc tgatgaactg cagtcattat ctatgccgaa
    13741 tatgatcctt caaccaatta tcgaaaacgc ctatcttcat gcttttcaca ataaaacgca
    13801 aggattcatc acggtgtacg gcaaaacaaa aaatcagctt cttttattcg aagtgattga
    13861 taacggcgat ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt tttcaggaat
    13921 cggcatcagc catgtagatg agcggatacg cctaatgtat ggcgatgcat atggcttaac
    13981 gatccaatca gtgattggga caggaacgac tgtaacgatt tgtctaccgc ttcttcctaa
    14041 aaatcaatca gaatatcaaa aataaagaat agccaagcgc tgattttttt attttaagtt
    14101 aaataaatca atcagtattc ttgactttca tctacgcgtc tcttctactg tcagcactgg
    14161 ataacagccc aatatttgac tttctcctcc gagcatcttg atttcttgga gcgcattctc
    14221 tactaatgtc attggacggt ccaacaacaa atcgatcacg aaaaaatatt ctcccaagct
    14281 agttttcaat ggtcgggatt caatcttact taagttgatt tcccgccagc caaaagctgc
    14341 cagcatttta tgaagcatcc ctggccgatt agcaggtagt gtcaagatga cactcatttt
    14401 tactggagct gactgctggc tggtcatctt tcggtcaccg acgatccaaa aacgtgtctg
    14461 gttcaactca ttatcttgga tattctcagc taagatctcc aatccaacat gctgtgctgt
    14521 ttctaatgaa gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat acattgctgc
    14581 agcagttgtt gattctgttg ctactaaagg aacattagga taatgcgttt ctaaaaactg
    14641 ttgtgattgt gctagtgctt gtggatgtga aaggattttt gtaatctttg ttgctggatt
    14701 gcctaaaagc tgctgcttga taggaagaac gatttcactt ttaacttcga cttctggatg
    14761 tttggaaaga agatcgattg tgtgatgaac agctccttcc aatgaattct ctacaggaac
    14821 gacagccaga tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga tggaagcata
    14881 cgcagtcaat tgttctgtag gaaatagctg acaagccgcc tgataagtga atgaactttc
    14941 tggccctaga tagctgacgt tcatatgctt ttgatccttt ctataatggt atcgatgatc
    15001 ttttctggtg tttgattggt cgtgttgatt cgatgtgtcg cactttcttc ataccaagaa
    15061 agtcgttgtg ccaataatgc ttttatttct tctcttgttt ttccaagagc taaaggtcga
    15121 atagacgttt catcttgttg gatacgatca actagacagt ccgcttctgc ctctaagtaa
    15181 agaacaatcg gctgtgtttt taaaaaacgc cgattttctg cactttggac aatccctcca
    15241 ccagtcgcga taattttgtc ttcatttgac agctttctta aaatatctga ctccactttt
    15301 cgaaagcttt tttcaccgta atagtgaaaa tattcggcaa tcgacaattg gatatgttcc
    15361 tcaatctttg tatctaaatc tacatactct ttttgtaaac attgagctaa ccctttaccg
    15421 attgttgttt ttccagcgcc catgaaacca atcagcaaaa tgctagccat gagcttctcc
    15481 tcctgacaca agttctgcta aatcgtcaaa aaatgccgga taagagattg aaacagcttc
    15541 agctccttct agttcgcaag gtccatcagc tatcaaagca gctacttgca gcatcatacc
    15601 gattcgatga tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg gcccatggat
    15661 gatcagaccg tctgctgtcg cttttatctt tgctcccatt ttctgtaatt cttctgccac
    15721 cgcatcaatt ctgttggttt cttttacttt taattcttct gcgtctttga tgatcgtgat
    15781 tccttctgct tgagtagcca ctaaagctag aatgggcaat tcatcaatca gacgcggaat
    15841 gatttctcca ccaatcgttg ctttcttcaa atgactcgtc ttgacactta aatcagccga
    15901 ttggttctgt tcattgtgat tcgtctgggt aatcgctgcg cccatctcct ccaacacatc
    15961 taagatacca gttcttgttg gattgacccc gacatttttt aacaacagtt ggctttccgg
    16021 aacaagaagt cctgctgcta gaaagaaagc agctgatgaa atatcacctg gaacagttat
    16081 ctgttgacca gttaattttt gcggtccggt cactatgatc gttttatctt ccactgtgat
    16141 ccttccgcca aattgacgaa tcatttcttc cgtatggtta cgggaacgtt ctttttcaac
    16201 gattacggat gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt tgacttgcgc
    16261 actggctaca ggcatattgt atcggatagg tgataaagag cgggttcctg tcacagtgat
    16321 tggtagatac tggtcatttt cttctccttg caagtcagcg cccatctcgc gtaaaggttc
    16381 catcactcgc cccattggac gtttagacaa cgaagcatcc ccgaacagag tagtcgtaaa
    16441 aggctgtcca gccaaaatac ccatcaataa acgtatggtc gtaccggagt ttcccatgtc
    16501 aattgattcg gttggttgga caaatgagtg gctaccgcga ccatgaatct tgatcacctc
    16561 ttcctcttct tcgatctcga cacctaattg ttggaaggca tgtaaagtac tcagacaatc
    16621 ttctgcgcgc aaaaaatttt gtatagtcgt cgttccttca gcaattgctc caaacatgat
    16681 gctgcgatgg gaaattgatt tatctgctgg tatcctaaca gtccctctta atccatgtat
    16741 ttgttgtaat aattgcattt atccagcctc ctatttgatc cagcaatgat aactggtatt
    16801 agacataatg attctttttg cttgtaacaa atcctgttgg tttttaaaac tgatttgcag
    16861 tattcctaaa atatcttccc tggtctcttg gatcttcaaa ttaatcaaag aaactcccgc
    16921 ttttccgatc agtccagtta cttctgcaat aacgccaggt tcatctggca catcgataaa
    16981 taaatcaaaa aaggcaggaa tcgcgccttg cttatggaca ggcaactggt ctcgactttc
    17041 ttttgcccga ttgaaaaaag aaaagatttc cgattggttc tcagtcatga tccattctgc
    17101 tatttgtgtc atttcttgtt gccaatctgc caatcgttcc aataatatct gcttattgct
    17161 caacaaaata tctgtccaca tgacaggatc tgaggaagcg atcctagtga tatctcgaaa
    17221 gcctcctgct gccagttgtt tggctcgcgg atactcttga ttgaattgtt ctgcctgatt
    17281 gactaagcca gctgccagaa tatgtggaaa atgacttagc attcctgtta tacggtcatg
    17341 ttctcttgct gtaagagtta catattttgc tttggttccg caaaatagtt cttgcagttt
    17401 ttcaacatct tttttcgtct tttccaaagg tgtaaagatg taataggcat tttcaaatag
    17461 tttagcatct gctgcctgta tacctgattt atgagaacca gccattggat gtccaccgac
    17521 aaaccgaaga tttttctgct cagcggatgt cacgacttgc tgcttcgtac tgctgacatc
    17581 cgtaatgatg acgtgatcag acaagttgat tctttcaagg acatcaagat aagctaaggt
    17641 tgtacggacg ggaacggcta ataagataac gtctgcttgt tctgcacctg tttcaaaatc
    17701 acttgcaacg taatcaacga tctgctgttc tactgcttgc tcacgggttt ttgactgact
    17761 atcccaacca actactatca gttctggatt ggtctgacga atacaaagtg ccatagaagc
    17821 accaatcagt ccaagcccca cgataaaaac atgttgtgcc atcttatttg cccccttttt
    17881 agtaatgttt caagtagtca cggtactcct tcactgcagc tttcaactca acaagcgaat
    17941 cacttgggaa ttttgacaat agctctgtcg ctaccacagt ggctacaaca ttttccgcaa
    18001 cgacgctagc tgcaggaact gctgtactat ccgaacgttc tacacttgct ttgtaaggtt
    18061 cttttgtatc gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg ggtttcatga
    18121 ccccgcgaat gacaagtggt tcgccattgg tcattccgcc ttcgaagccg cctagattat
    18181 tggtacgtcg gaaatagcct ctttcttctt cccataggat ctcatccatc acttctgagc
    18241 ctggtaatga accagaagtg aatccgaccc caaattctac gcctttaaag gcattgatac
    18301 taaccacagc ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg tagcttccta
    18361 atccagctgg aacgcctcca acgagcactt ctacaattcc tccgatcgta tctccatttt
    18421 tctttgtctg atcgatcaaa tcttttattc tttgctcaac tgctggatca acaactcgta
    18481 cttctgacgc ttcagaaatt tctttgattt cttgaatcga gatatttcca ggtatttctg
    18541 cttggattcc accaagacaa atcacgtgtc ctgctacgat gatgtcaagt tcttccagaa
    18601 ttttcttggc aattgcgccg attgctacac gcatggtcgt ctctcttgca gaggaccgtt
    18661 caagcacatt ccgaagatct cgatgatggt atttcatacc gcctaccaaa tccgcgtgtc
    18721 ctggacgcgg acgtgcaact cgccgcagtg attggtcttt ttcaggcact tctgctacag
    18781 acatgacagt tgtccaattt ttccaatctt tattttctac cactaaagtg acaggtgaac
    18841 caattgtttt gccgtgacgg atgccagacg tgatccgaac acgatctttt tcgatcttca
    18901 tccttcctcc tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg atttcttctt
    18961 ctgacagagg taatcctgct ggtagtcctt caataatggc tgttaattct ggtccatggg
    19021 attctcccgc agtcatataa cgcatcttac agtcctccta agtaatcttt gaatgtctct
    19081 gtgggaacag gaacgatttt tgcttgccca atcttttcta gcaagatgat attcaattgg
    19141 cttcctcttg ctttcttatc atgggttatc gcgtggaata attcatctgg atgacttaga
    19201 tcgtacgtaa tcggcagatg gaatttttga agtatccttt ctaaagcagc tgtcgatcca
    19261 gttggagaca agccgatttt ttctgcatga gaagtgatgg ctaccattcc aatagctact
    19321 ccttctccat gactgactac cccatatcct gctgtatttt caattgcatg gccaattgta
    19381 tgaccaaaat ttaaaataag tcgatttcct tggtcaaaga catcttcttc cactactttt
    19441 cgtttgattt ccaacgtagc agtaatgatt ggaacagcat gttccagaag atctttttcg
    19501 tctttcagct cgttcagctg tttccaaagc gttgcatctg ctatagctgc tgatttgatg
    19561 atttcagcga ttccttctcg taaacgacgt accggcaaag tttttagaac ctcaggatca
    19621 atcaatacac cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt gtttgtattg
    19681 actgctgtct tcccgccaat actagaatct acttgtgcaa gtagcgtagt cggaatttga
    19741 acgaaatgga tcccacgcat gaacgtcgag gccacgaatg ctgctagatc acccaccatt
    19801 cctccaccaa gtgcaagtat cccatcgctg cgggtaaaat cattatctgc taaaaaatcg
    19861 tataactcag ctgcttgttc taaagattta ctggtctcac ccgccggtac gattgaaagg
    19921 actggttcaa aattagcttg tctcaattgg aacatgattt tttctgcata tagtggagca
    19981 acgtttgtat cagtgatcac agctactttt tgaggtctcc aaatcgtttg aagccatgat
    20041 ccagcctgtt ctagtgcgcc tttttcaata tgaatatcgt atttttggtt atcaacatgt
    20101 acagtaagca ttcgtttcac ttctttcttt taaaaagctg gaattaatcg agtgtctgaa
    20161 tcttttccat tgcttctttc atgatcgcaa cttctttcat catgcgtgag taagtctttt
    20221 cattcaacga ttggggaccg tctgaccaag catttgcagg atcaggatgg atctctacga
    20281 tcatgccatc tgctccgctt gccacacccg cacgagccat tggtggtaca agttcccaaa
    20341 ttcctgtacc atgacttgga tcaacaatca cagggaaatg agttaatttt ttcatcaatg
    20401 ggacagcact taaatcaaac gtattacgtg tcgctgtttc gtaagtccga atcccacgtt
    20461 cgataaaaat gacaggagat ttgtcttcga ctgcaatgta ttctgcagca ttaagccatt
    20521 cattgatcgt accggaaatt ccacgtttca atccaacagg tttacctgtt ttaccgacag
    20581 cagatagcag tttaaaattt tgcatattac gtgcgccaat ctgtaggata tcgctgtact
    20641 cagccaccat atcgatatgc ccttcatcca tcacttcagt aatcactttc attccaaatt
    20701 catcagccgc ttgacggatg tatttcaatc cttcttcttc taaaccttgg aaagcatatg
    20761 gagaagtacg tggtttgaag gcaccacctc gcagaatttt tgcgcccccg gctttggcca
    20821 ttcgcgcaca ttctctgatt tggtctaatc cttcgataga acaaggacca gccatcgtca
    20881 cgaaactgcc gtcaccaatt tttactccgt ctacatccac gactgtattg ccaggatgga
    20941 attctcgaga agtcaatttg tatgtgttta aaatacgcac agcattttct actccttcat
    21001 agcgtgtaaa tgctccttct tgtaaacttc gcgtatcacc ttttagtcca atcactactc
    21061 gttctgttcc ttgatcgatt gagactttga atccttcttt ttcgatatga tcgacgactt
    21121 tttttatttg ttcttccgtt gcttcttttt tcataattac tatcattgtt tttcccgctt
    21181 tcttttgttt acttatctat cttggctgat tttctctact ttttcaaggt ggttttacat
    21241 taattcctac taaatgaccg tgtcctgttg ttctcatttt cgctccttct ttcctactta
    21301 tgacccagat aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta cagtgaacat
    21361 cttcaacgag ccttaaaaag cgctgcaaaa atataaaata gccactatag atgaatttct
    21421 ctacgtggct gtaagtcgtg tttaaaaatc tttagccatc atagatacaa acctggtttg
    21481 tttgtaccca cgaagtcatg aatacaaacc tatctaaaat agctaaagta atacgtattc
    21541 agttttgtaa gttttctaga tttcataaaa aatcgctcca aactgatgta agaattgtac
    21601 ctagtataga cataaaaaaa tcgactgtca atcattttta tgaaactttt tttaattttc
    21661 tgatagttaa ctttgttttt cagaaaaata acaattgatt ttattgaaga aaatagtcat
    21721 gaacaaaagt atctgagaac ttttttactc tcttgttcat gacatataga ataaagcagc
    21781 tttgtcttgt aaactccgtt tttcaaataa taatctaaag attgctactg aataataaag
    21841 ccgatttcat ggtcctcttc gctgctgatt cggtaaggtg actcaacatc cgctccccag
    21901 ctattgatac ccccgactcc tcttactttt gcaaaaatcg taagaaccgt tcgtctttct
    21961 ggcggtagtt cttctatatg tgttgcctgt tccagttctt ctgctttgaa tggtaaagcg
    22021 gaaaaagcaa atggcgtatc ctgctgttca aatcgcaacg aaaaatcttc ttgatcttta
    22081 tccgtattat ccaacgtttg atttcttgtt agttctaccc atttcgtttc catatgcatg
    22141 ccgcattctt ggggaacaag gtatggggaa acggggagtc ctgccacttt ataaatacct
    22201 tcgacaccac cagccatccg atccggatat gtttcccctg ataggccttc atactcaaaa
    22261 aattttgctt tcgttggcat aatgaacctt aaacccaatg cgggtaattc cggtaatcct
    22321 ttttgtccat aataatgcat attgacaagc attttaccct ctccagcgat ttggtaggaa
    22381 attgtcactt gggtttttgg tactgtgttg atttccagtg taaaaatgat ttcaaccgtt
    22441 tcagcgtatt cattacttga atattgatta tttactggag caatcggcaa atcgatcttt
    22501 ttattgtcca ccagtacttc tatttctttg acttttggat acaatccggc tcctaaccac
    22561 ataccggatt taaatccaaa tccgctacct ctgtcattat cggtcgttgc tcgccaaaaa
    22621 actggaagtg tttcccggta tagccattct tttccgtttt tattgagcga ttccagtccg
    22681 cctcttgtat aagaaaaaat ataatgcgta ttccccgatt tgacacctaa tgtacagtcg
    22741 ccgaagacga ttgcaacttt ttccgtatcc atatgacttt tatttactgt atttttcata
    22801 ataatatctt acctcctgca tggcgggttt ttcttgtcca tctgcaaaaa cgatcccatt
    22861 ccctgagaat tcatagtcac ttggtttatc gtcaaagtca ccgccatagc gcatcacttc
    22921 tctgcctgtg attggatcag tcacttggat cgcctgatct ttaaaatccc atatatagcc
    22981 tccttggtac atttcataac gatcaagcaa cgtcatgtaa gaatccattc cacctaacga
    23041 attccccatg tcatgcatat attcacataa gatgaacggt ttttccgctt tattttcaag
    23101 gtagtcaatg atctcttctg gtggcgcata catgcagctt tttacatctg aaatcgaatc
    23161 ttcgaatgct ggatttggaa aattcccttc ataatggaca agtcgatcgg ggtctacttt
    23221 tttgaagtaa tcgttcattt gtcgaatgtt ttcttccgca taagattcat ttccaagaga
    23281 ccaaaaaaga atacttggat ggttttttaa tgtttcgaaa ttcgtttttg ctcgatcaag
    23341 aacagccgcc tgccactgag gtactgcacc tggtacgttg taagaaggct caaatgcgcc
    23401 cattttttgc catgagccat gggattcgag gttcgtttcc gccatcacat ggattcctgc
    23461 ttggtcgcat aaataatacc aagtgatctg gttggggtaa tgagatgtcc gtacgccgtt
    23521 gatctttgca ttttttatga tagccaaatc tttttccata tcttctatag tgatggcgcg
    23581 tccttttttc ggatgccact catgtcgatt taccccgttg ataatcatcc gttttccatt
    23641 gaagtagatg attttatctt tgatttcgat tttacgaaaa ccgaaggaat agtcaatata
    23701 ctccgttact cttttttctt cgtctatcgt ctccaattca agcttatata aattgggctg
    23761 aaaatgcccc cacagtgcca cattttcaaa tccatcgatg cgaatcgtca tttgctcttt
    23821 taacggatat tgctgttgat agatttcctt cccatcagga tctagtatcg ctacattcag
    23881 ctgactgttt ttggctactt gctcaaaaga caaatctact tcaagtttgc cattttgaca
    23941 tttgtcatct aagcgaggtt tgatccaaag atcttctaaa tggttttgtc ctcgagcaac
    24001 taagaagaca tctctgaaga tcccaaaaaa tcggaaaaag tcctgatctt ccaagaatgc
    24061 agctgtactt cttttgtaaa cctccacagc tagaatattt tcttcttcca aataaggtgt
    24121 taaatcaaat tcagacggag tgaacgaatc ttcggcatac ccgataaaat gaccattcaa
    24181 ccaaataaac attgcttgtt ctacaccagc aaaatgaatc gagatttgtt ttccctttag
    24241 accttcttct aatcgaaaag tcgtcaaata cgaaccaacc ggattgtctt tagcagctga
    24301 aaagatccct tccgcctctt tctcagggct tgtcgtgaaa gggggacggc gaaatacatg
    24361 tccctcccac ggatacatcg tattgatgta atggatcttg tcataatttg ctaattcaat
    24421 atgactagga acagtgattt catcaaaaga acaggcatca aaatcttttt tataaaatgt
    24481 ttttggtcgt accgaaggat ttggcgaata acaaaaacgc catgatccat ttaaagactg
    24541 tttaagggag gatgtggttt ctacttcttc ccttgttgca taacaaacat gatcgctatg
    24601 tgcatttgat tggttcactc taaaaacttg tggatcatct aaccatttta tatttgcttt
    24661 catacttttt tccttctctc tcataacttt ttgaaatgaa gcagttctgt ttggaaatca
    24721 ccttgaaaat cggggaagta aagtccaact gtcatcaatt cggcccccga aaatatccgc
    24781 ttcgaggtct gttcctgata aagtgcctca tcatctaacc ccatcagata tacttcatgg
    24841 aacgctggtt gagctgaagc tagtatcctc cctaaaaaca caatagcttc atcttgttta
    24901 ggagacacaa acatccatgc ggcaatattt tcttcaaaag gactcgccaa acgataataa
    24961 tggccatact ggatcaccgg acgaatcgtt tgataggata taatttggtc ttttacttgc
    25021 tttttttctt caggagacag ctttgtcaaa tccagttcat aaccaaaaac agcgctcata
    25081 gctgtttctc ctcttgtttt caacgaagta gatctgtcgg tctgatgatt tggtacagct
    25141 gatacatgag ctgtgatgga cgaaatcgga taagcaaggc ttgttccata ttggattttc
    25201 atacgctcca ctgcatccgt gttatcactt gtccatgact gcggcatata ataaataaac
    25261 ccggcatcaa accgtccacc tccaccagaa cagccttccc ataagatttc cggataatca
    25321 gttgtcaaat gttcaagtag ttcataaagc ccgagcatat aacgatgcgc tacttctcct
    25381 tgtctctgtg ggtcaagggt aatcgaatag acatctgaaa gcgaacggtt catatcccat
    25441 ttgatgtagt caagaggaat cgtatctaaa atctttctca tttgtttctc aatcgtctgc
    25501 cgaacatcta accttgtaaa atccagtaca tgttgtgatc tggaagcaga aggcattcta
    25561 cctggctcct gcataagaaa atcaggatgt gttcgataca gttcagaatc aaccgagatc
    25621 atttcaggtt caaaccataa accaaatttc aatcctttgc tatgagcata atctgctatt
    25681 tcacgtaatc catttgtcaa cttgccttca tactcgaacc agtctcctaa tgaagaattg
    25741 tccgcatctc tttttccaaa ccagccatca tctaatacga acatttcgat gcctagctcc
    25801 tttgcttcat caatgatcgc cttgattttt tcactattga agtcaaagta agtggcttcc
    25861 cagttattga ccagaatcgg ccgttcagca aattgatgac gacctcttgc gattctttca
    25921 cgtagcaact catggtggat ctgactcatt ttgttcaatc cctgacttga gtaagaaagg
    25981 atcatttcag gtgtttgaaa agactggcct gcattcaatt cccacgaaaa acgatggctg
    26041 ttgatcccac cgacgactct caattgattg atctgatctt tttcaagttc gaaagaatgg
    26101 ttgccggaat aaacaaattg aagtccgatc gcttctccag tattttctgt cgtatgatga
    26161 tgaactaaag caataaagtt attcatatgg tgactgctcg ttcctcgacg gctttcaaat
    26221 acttttgttc cagataaaac agattgccgt gaaatctgtc tttcattgac atgtgctcct
    26281 ggcaaagcaa tgacttcatc aaatcgccta gtatgggcaa agtctagctg gaaactagcc
    26341 gctttttcta gtttgattgt ttctccggtt tcatttctta tctttacaga tcgtgtgatc
    26401 actgctctat cagtaaatat ggtatagaac aaatcgaaat aaatcgctgt ttctctatcc
    26461 ttaagtgaaa tcaccaacgt ttgtgcctct gacttgtcca tcacataagt ttgaggcaag
    26521 ccttcgatct ctggcttccc atctgtaata taatggctgt catatctcag atccagtaag
    26581 tctgatccat tcttgcgctg aatgatcgat gcgggcgttc gaaagtccat gctgccatga
    26641 gaagagtatt cttgcggtag tgtatctttc gacagagacc ggtcaggatt taagggaaca
    26701 tttcctgaaa atccacgatc tcttcgggga taatttttat tgtctttgta ctgcttgact
    26761 ggtttgccaa aataaatatg cgccaataca ttgctttctt ctatctttat cagataagaa
    26821 agaaatgtat ttcgtaaatg gaagactcgg ctcttttcat caaataagat gaaattttta
    26881 tctgtttttc tatgcgtaat atccatcatt tcactcccaa caaaacggaa accgtttact
    26941 tttttttata attcctgtat actgtttact aaacaatttt gtcaataaaa attttttatt
    27001 tttttgagga gtgtgtaaaa atggttggaa ttagagatgt cgcacgaaag gcaaatgtct
    27061 ctcctgctac agtttcccgt gtgttgaata acgatcagac gatccatgtg gctcagacta
    27121 ctcggcaacg gatagtagag gcagctgctg cgttggatta tgacatcagt actcgtaaat
    27181 atatcaaaaa gagaatgcct tcgattggtg tgatctctac gatcggcaaa gcgagcgaag
    27241 aacaggatat ctattacaaa catttgcgag tagggatgga agaagaagct cgacgtctgc
    27301 atttagggat gaatcgaatc tataatctat ctgataatcc taaacaatgg gaagatctag
    27361 atcagttagg tgcggtgatc gttgttggga ctgttacgaa agaatcgatt accgccttgt
    27421 taaaacaaaa catgcaaata gtcgtcgtgg ataaccctga catccagcaa gaggtcgata
    27481 tggtgtatgc agatcttgaa cggatgacta aaaccgtact ggaattgttc ttagaaaatg
    27541 gtcataaaag aatagcctat atcgggggat ataaagtcga tatggatgaa ttcggtggga
    27601 agcagatttc tataaatgaa aagaggatgc gtgcctatca gcattttatg atcgatcatc
    27661 acttagaaaa atatatgtct tatcatctag gtggctggac agaaaaacaa ggtgaggtac
    27721 ttgcgactaa tctattagaa aatgaatccc ctcttcctac tgccattctt gtcgggagcg
    27781 atccaatggc aattggtgtc tatcgggcat tgaaacaaaa aaatatccaa attggagaag
    27841 agattgttgt agcaagcttt gataatatcg agacaatcgc aaacctctcc cctggcttga
    27901 ccagtgttca aatcaatgcg aaatcaattg ggcaagcagc tgttcgaatg gcattggagc
    27961 gaattgacgg tatacgggac gaaccaatca ttttgactta tcctgcacga ttgattgtta
    28021 gggaaagttt tatcccaaga acaaaaaggt attgaaagag aggttgtgaa aagccgcttc
    28081 gtttcttcat actcaaaacg aaacggcttt ttcacaacct cttacttata cttattcaca
    28141 aaatccaatg tctgctggta acaattttgc gattctttta gcgtgtaatc aaattggtat
    28201 tcatgggtaa tctctttttt tgtatctgta taaaacaagg attgcacggg tacctttaat
    28261 tctgtcaacc gattttctaa tgcttgacct tgctcctgga atgagtatgc atttccgtca
    28321 gttatatacg ttggcggaaa ttccttagaa acatggtcag ccacactagc ttctttcaat
    28381 tgctcactcg ttttccaatc ttttgttcca atcaacgacc acgcaacggt tttgacgaaa
    28441 aacttcataa atcgatcatc cgaatgctgc tgtgctgtta gcttcaaatc aacaggtccg
    28501 caataagaaa tggttccttt cagactatct tttggaagta gctgtttgat tttcatttgt
    28561 ttagcatacg catcattcgt ttgtatagct gcatattgca atgcgatttg tgaacctgca
    28621 ctatctccac caaaaaataa ttgttccatg tctagcaatt tgtcgtcttt ttcttcagct
    28681 aataaatctt tgatcagttc attcacttga agaacttgat tagggtattc tgaatcaggc
    28741 gctaattcat aattcatcgc aaccactgcg atgttggcgt ctgaaactaa ttttgtcgca
    28801 aattctttga cacccgattt atctccgccg acgaatcctc caccatgtac ccaaaataga
    28861 acaggaacag gccccttgct gttttttgga tagtagatgt cataagtatt ctctttatac
    28921 ttggactcat acgtcaaatc tgtttttttc gccacgtttt tttctgcttg atcgaacgtt
    28981 tttttatcag tgatttgaac agtatttgaa aacatatggt tgatcaagaa tgctccaggt
    29041 ctgggggaaa gctggaaggc aagaaagatt gcaaacgcga tcccaagaat gattccgaag
    29101 aaccatccga taaatttaag aattttcttt ttcataaggc tccctcagta gttagtatta
    29161 agcattctga ttataccata gtgttatttc ctatcaagct ctcctagatg atttaatgat
    29221 tttccccaaa cttcttctgc aatgcttttg acaaaagaaa ttttttccat tgttcttcaa
    29281 acaaaacatt ttcttcttct gttgaagcaa atccacattg aggacttaag cacaaacgat
    29341 ccaaaggcag atatcgtgtc gcttcgtaga tccggtcaat cacttgctgg tgctcttcca
    29401 tttcgccatt tttcgttaca gtcaaaatct aagacaacta atttatcttc agagactttc
    29461 tctaaaagaa caaaactccc agcacgttca ctatcgtatt ctaaataata ggcacggaca
    29521 ttttcttgag taaaataatg ggtctgctac gtcgtcatcc cccctgatgc cgccaaaatc
    29581 gaataataat ttccgcgtca aacatgtata tttagtgttg aatcatttga gaatatgaaa
    29641 ataaaaatct ttttgatcaa tcagttaatc aaattgaaat aacgaaaaat ctgagacaga
    29701 atgacgttca ttctgaccca gatttgattt tgcacaactc aacagtcctt attggcaaat
    29761 cgccaaaata atgtagacta actaaaaaga ctaagaaaca ataacaaaag ttaacacttc
    29821 ttagtctttc tttagcacgt ctatttatta aagattttag ggccttttct catgttttta
    29881 cgatcattct taggtgtttc catttgtttg gatcggcctc caccttgttt ctttttgatc
    29941 aattctttca ttttttcttt ttggtccatt tttattctcc tttaacgaat gaagcgacta
    30001 attcgatatg atgggtttgc gggaaaagat ctacaggctg aactttcttc acggcataac
    30061 ctgcttctgc aaataactta gcatctcttg caaaagttgc cggattacat gagatataga
    30121 cgattcgttc tggtgacatt tcgattgctg attcaataaa atgatgatct aatccttttc
    30181 gtggagggtc aactacgatc acagatggtt tgatcccttt ttcttgccat tttttcataa
    30241 ctttttctgc ttttccgact tcataatgaa cattttcgat tttattattt aaagcattga
    30301 actgtgcatc ttcaattgct tctggaataa cttccatccc atacacctta tccactctgt
    30361 ctgctaaaga caagccaatc gtacctattc ctgaataagc gtcgatcaca atgtcttttt
    30421 tatctaactc ggcaaactcg atggctgttt ggtacagata ctctgcttgt tgagtattta
    30481 cttgataaaa tgactttgct gagattcgat aggtttttcc taatagctga tcttcgatat
    30541 agtctctgcc atataaaact ttttctttgt ctcctaaaat cacatttgtt ttttcttcat
    30601 tgatattttg gatgaccgaa accacttctg gcaattcttc atgaatagct tcagcaattt
    30661 ccttggattt aaagaatttt tctttacgag taactaaaac gaccatcatt tcatgggaat
    30721 aatgaccacg acggataatg atgtgacgaa gctgtccttc atttttttct tcattatatc
    30781 ctcgtacttg gaaccgttgc aagatatctc tgactacttt gattgcttga tcgatgacag
    30841 gatcttggat ataaaaatct tcgataggga ctagatcatg gctattcttt ttgtaaaaac
    30901 cggtggctaa ctgtccatcg atacggcgaa cagggatctg tgctttatta cggtagccga
    30961 atggttctgc cattccgacc gttggaagta caggaacttc tggcatctta gcaatcttcg
    31021 ccattacatg ctctacttgt ttttgtttga agacaagctg ttgttcatac ttcaaatggg
    31081 ctaaaggtgc gatacctatg cgaagtaatg cattgttttc tacaggctga cgatcaggtg
    31141 attgtgtaat aaatgtttcg acttttccat atccaaactt attgcctgct tttactacta
    31201 gtatttctac ttcttctcca gggagtgcat tttcgataaa taatggataa ccatcgattt
    31261 ttgctacccc cattccttca taactcaagt cgacgatttc gactgtatat cgttcgtttt
    31321 tctttactat ttgctgttct gtcatatttt gctcctttaa gcctattttt caatcctact
    31381 gaactctact ataaatcagc taattacaca actaaaaccg gcactgacct tactacgttc
    31441 agcaccggct tttgattaat tcttttgatt aggtgtttcg tctgctattt ttccgtcacc
    31501 gtcgatatct tcatccgtta aacgttcgac ttcttttacg aattctttgc tgacaacctc
    31561 taattcttct tcatctgtac ctgtgattgc ttcatcagga atctgatcgg tattggcaaa
    31621 gaactcaatg tgttgatgaa gattttggaa atgcatcggt gcttccccgc cgtattcacc
    31681 atctaagttg atcattactg gtcgattgat atcatcttct gtttcaacga acaaactgct
    31741 tgttttggta tagattattc ttggatcatc gacatgtcga ccgccattca gcatgagtgc
    31801 tgcaatatgc aagatctcaa agatatttgc tgtcttcaca atgattaaag aaaacttccc
    31861 atcatccaat ttggcatctg gcgcgatctg ttcgaatcct ccaacagagt tcgtcagccc
    31921 taggaaaaac atcgaagcat ttccgatata ttccccttca tcatacttca agcgcatttt
    31981 gattggcttt actcgaggga gcatctctgc tcctttcgca agataagcaa gataaccaaa
    32041 aatactcttt agttctgatg gaacttcata agtcaattct gtcaaatagc cacctgcagc
    32101 gatattgatg aagtaactgt tatcagaaag gccgatgtcc atttttacgg tttggtgttt
    32161 ttttatcact tctgctgctg ctttaatatt atctctagga atttttaatg ctcgagcata
    32221 gtcgttcgtt gtaccagctg ggatgatggc cattttagga cggtgttcaa gcggagcgat
    32281 tccattaacc acttcgttga ttgtgccatc gccaccagct gccactacta gatcaaaacc
    32341 tagttctgcg actctacatg cttcattctt ggctgaatct ggttctggtg tagttgcata
    32401 ggcgctggct tcaaatcctg cttcttctaa tgcctgtagg atatccgcca aatttttttt
    32461 caataattct tttcccgagg tggggttata aattacgcgt gcttttttca ttttcagcct
    32521 ctatcgtttc gctaattcat ctttaagtag cttattcacg acaccaggat ttgcttgtcc
    32581 tttagttgct ttcatgattt gaccgactag gaagccaacc gcacgatctt ttccattttt
    32641 gaaatcatcg acagattgtt ggttgttatc caaaatttca ttaatgattg gcagtagttt
    32701 gttcggatca gataattgca cccaaccatt tttttcaact acttcttggg cttctccgcc
    32761 atttgtgatc aattcacgga agactttttt ggcgattttt gagctgatcg ttccatcggc
    32821 gatcaaacga atcattcctg ctaagttctc aggtgtcaat tttgtctcag ctaattccag
    32881 tttctcactg ttcaagtaag cagaaacttc acccatcaac cagttagatg cttgcttggc
    32941 atctgcacca ttttccaaaa cagcttcaaa aaaatcagac atttcttttg taagtgtcaa
    33001 taccattgcg tcatactctg gaagtccaaa ctcacggata tagcgttcgc gacgagaagc
    33061 tggcatctca ggcaaactag cttttacttt ttgaatccat tcgtcatcga tcacgaattt
    33121 cgggatatca ggctctggga agtaacgata atcactagac ccttctttga cacgcatcaa
    33181 aactgtttta ttcgttgttt cgtcaaaacg gcgtgtttct tgttggattt ctcctcctga
    33241 aagaagaact tttgcttgac gtttttcttc aaaagctaaa ccttttttca cgaaagataa
    33301 agagttcaag tttttcaatt ctgctttcgt accgaactct tcttgtccat aagggcgaag
    33361 agaaatattg gcatcacagc gcatagagcc ttcttccatt ttcacatcgc tgacttctgt
    33421 aaattggata attgatcgca atgcttctag ataagcgtat gcttcttctg gtgaacgcat
    33481 atcagcttca gaaacgattt cgatcaaagg cgtgccttgt cggttcaaat caacgtaaga
    33541 ataaccgcca ataccatgca tgttttttcc agcatcttct tctaaatgga cacgttcgat
    33601 ccggattttt ttctttttac cttctacttc gatttcgatc catccgtcat gaccgattgg
    33661 ttgatcaaat tgggaaattt gatatgcttt tggattatct gggtaaaagt aatttttccg
    33721 gtcaaaatga gtatctttag agatttcaca gtttaatgcc aaagctgctt tcatcccgaa
    33781 ttccagcgca gctttattca ttactggcag cacgcctgga taaccccagt cgatcacgtt
    33841 tgtattgctg tttggttctg caccaaaatg agctggcgct ggagagaaaa tttttgagtt
    33901 ggtttttaac tcgacgtgga cttctagtcc aatgactgtt tcaaaattca ttcgtctttc
    33961 cctcctaaaa tagctggttg ttgtttatgg aattctgttg cttgttcaaa cgcatatgcc
    34021 gcttgataca tcgttttttc atcaaaataa ttaccgatga tctgcaatcc aactggtaat
    34081 ccttctgata aacctgccgg tacagacata cctggaagtc cagccaagtt gacagggatc
    34141 gttaagatgt cactcatgta cattgtgatt ggatcgttaa tgttctcacc gattccgaat
    34201 gcaactgttg gtgaagatgg cccgatgatc aagtcataat ctgcaaagac tttttcaaaa
    34261 tctcgtttga tcaatgtacg gacttgtcct gcttttttga agtaagcgtc ataataaccc
    34321 gcgcttaagg agaatgtacc taacatgata cggcgtttta cttcttcacc gaatccttct
    34381 gaacgtgagt tgacatatac atcatctaat gatttcacat tttcagagcg ataaccgtag
    34441 cggataccat cgaatcgttg caaattggaa cttgcttctg atgaggcaat gatataataa
    34501 acagccacac cgtattttga atgaggcaag ctgacttctt ccacaacagc acctaatgaa
    34561 cggaatgttt ctgctgcttt caatacagct tcttttacgc caggggccac accttcgcct
    34621 aagtattctt taggtaaagc aatcttcatt cctttgatgt cgcctgtcag tccctctgca
    34681 aaatcaggaa ctgaaacgcc tgaagaagta ccgtcttttt cgtcataacc gctgatagct
    34741 gtcaatgcta atgcattatc tttcaccgta cgggtcattg gtccgatttg gtcaagagat
    34801 gaagcaaagg caatcaaacc aaaacgtgag acacggccat aagtaggttt catccctacg
    34861 atgccgttaa aagctgctgg ttggcggata cttcccccag tatcactacc taaagaaact
    34921 ggaatttgtc cagctgcaac tgctgctgca gaaccaccag aagagccacc aggtactttt
    34981 gttgggtccc aagcattttt tgttttcttg aaataagaag tttctgtact tccacccata
    35041 gcaaattcat ccatgtttag cttaccaaca gggatcatat ctgcttgata gattttatcc
    35101 atgaccgttg catcataaat tggttcaaaa ttgtaaagga tcttagatgc agcggttgtc
    35161 aagatatctt ttgttacgat attatccttg ataccaattg gaataccagc aaggacgttg
    35221 ctttcatcaa tccctttttc atctaaagct tttgcctgtt ccaatgcttt ttcttcgttc
    35281 aatgtaatga atgcctcgac ctcaggctct gtttctttga ttcgttcaaa tgttgttttt
    35341 gtcaaatctt gtacagtgat ttctttcgat acaagcatat gatgcagttc ttctagagaa
    35401 cgattgtgta attctgtcat tatgcgccag cctccccatt atcgatgatt gcgggtactt
    35461 tgataaagcc gtcttctgtt tccggcacat ttttcaataa ttcatcgcgg ttccatccag
    35521 cttgcgcccg atcttgtcgc atcacgttta ctgtttcaac cacattcgac gtaaatggaa
    35581 caccttccgt atctacttct tccagcattt caaccatatc gatgatttta cctaattggt
    35641 cggtaaaact tgcaagttct tcatctgcga acgcaagttt tgctaatttg gcaacgtgtt
    35701 tcacttgttc ttcactaatc gccatactac cccttctttc taaacgagtt aaaaactttg
    35761 ctctctattt taacacaaga gtgccgtgtc tgaaattgtt tattgaaatt tgtctgaaaa
    35821 aactcatttt tcattcttct actgagcgac ttttagccaa tgcatctgcc atttgatctt
    35881 ctgtccaaac ttcgattcct aattcttgtg ctttggtcag cttgctgcca gcatcttctc
    35941 ctgctaccac gatatccgtt tttttcgata cgctaccagt cactttcccg ccaagatttt
    36001 cgatcgtctc ttttgcttct tcacgagtaa atcgtgttag tttaccagta aggactactg
    36061 ttttttcttt aaaaggagac tcgacttctt gcaattgggc atttcgaagc cctttgtatt
    36121 cgaaattgac gcctgcttgt tttaattcat tcatcaattc atggacttct tcgttactga
    36181 aataagtgac tacactgtcg gcaatgatat caccgatcgt atcaagcgca ataatcgact
    36241 catagtcact tttactcaat gtttctagat caccgaaatg ttctgctaaa atttttgctg
    36301 ctttcgctcc gacatgtcgg attcctaacc cgaaaatcaa ccgctcgacc gaattatctt
    36361 tgctgttgtc gattgctgtc aaaatattat ttgctgattt ttcttttatt ttgtccaacg
    36421 tcaataactc ttcttctgtc aatttgtaaa gatcagcaac atctgctact agttttttat
    36481 catacatctg ttctagtacg cgcggcccca acccatcgat attcatcgca tttcttgaaa
    36541 caaagtggtt caaaccttct ttcatttgag cagggcattt aggattgatg caccgtaagg
    36601 ctacttcttc atccaaatgg acaagttcac ttccacaaac tggacaatgt gtaggcaatt
    36661 ggtattcttc gctgtcttta ggtcgtttat ctaacacgac ttgcgaaact tctgggatga
    36721 tgtctcccgc tttataaatc agaactgtat ctttcaagcg gatatccttc aacttaatat
    36781 aatcaccgtt atgcaggctg gcacggctta ctgtcgttcc tgctactcgg actggtgtca
    36841 tgatagctgt tggcgttact acccctgttc gaccgactgt ccattcgatg ttttcgatta
    36901 gcgtttcaac ttcttctggc ggaaacttgt aagctgctgc ccatctcggt gctttgatag
    36961 taaaaccaag ttgatcttgt agcgagaatt cattcacttt gatgacgatt ccatcgattt
    37021 catagggcaa atccacacgt ttgtcatgat attcttcaat gtatgaccac acttcatcaa
    37081 tcgaatgaca tagacgtttt tcccgattcg tgtggaatcc aattttttct agctcttcta
    37141 gcgcatcata ctgggttttt gcttgcatag gaccgaaatc agctactgtg tataaaaaag
    37201 tgtccagatt tcttttcgct gtgattttag aatctaactg acgcaaagaa ccagctgcgg
    37261 cgttccgagg attcgcaaag atatcttttc cctctgcttc tctgtcttgg ttcaattgga
    37321 caaatgaccg ttttggcatg aaacattcgc ctcttacttc gatgttcatt ggttctttta
    37381 atttgattgg gaccgaccgg acagttttta ggttttcagt gatgttttct cctacagttc
    37441 catccccgcg agtcgcacct ctcacgaaat ttccatcttc ataacgcaaa gaaacggaaa
    37501 gtccatctat ttttagttca cagcaatagt caactacacg tcccactgct ttttgtacac
    37561 gttgatcaaa agctatcagc tcttctttgc tgaaaacgtc gttcaaactg taaagcggaa
    37621 tatcatgagt gactttttca aaaccttcta atacttttcc gcctactcgt tgagtgggag
    37681 aatcagacgt gatcaaatca ggatattgtt cctcgattgc tactaattct gcgtattctt
    37741 tatcataaac atagtcttcc actgttggtt tatcttctac gtagtattcg cgagaccatt
    37801 ggttcagtct tgtacgaagt tcttctgctc gtttagcagc ttcgtcaatc gtcatttcat
    37861 gttccatcat ttaccctcca ctactaagct ttttcaattg gcgcaaaggc agccaaaaga
    37921 cgttttactc cttgacttgg gaaagccaca tctagttcta gatccttcga cgtcccgctt
    37981 actcggacga ctgttcctag accccatttt ttgtgtttga ctttatctcc aggtttccag
    38041 ctgttatttt cccctccagt agcactttta gagggaacaa ccgctgtctc tggctgttta
    38101 tagctatatg ctgttttttt agcgaataca ggcgtttttt taggcgtagg acgcatgcct
    38161 tcgatttcca gtaactcctg atcgatttct tctacaaaac gagaaggtcg gttatattgc
    38221 gtccgtccat ataatgttct ggagaaagca tttgttaaat acaacgcttc ttctgctcgc
    38281 gtgatcccca catatgcgag acggcgttct tcctccagtt cgctttcttc catcaatgct
    38341 cgagataaag gaaaaacccc ctcttctaga ccaatcagga atacaactgg aaattctaat
    38401 cctttggctg catgcaaagt catcaatgtc acttgggaag catcttcttc taaattgtca
    38461 atgtcggaaa caagtgccag atcatttaag aagaccgtta gtttttcttc tggtgcatca
    38521 gcgtcttctt cattttgctg ttcgaattgt ttgtcgaatt cttgtgtgac cgttaaaaat
    38581 tcctccaagt tttcgatgcg tgcttgtgct tctaatgtat tttggatttt taagtcttct
    38641 agatatccgc tacgatccaa aacctctttc gtcaattctg tcactgtcaa gtatgggatc
    38701 atctgagtga cttcttggat catctctcca aatgcaccta attgctgtcc tgcttttcca
    38761 ccgatatttg ccagatccac attttgcgct gcttcaagca gtggccattc atgaagagaa
    38821 gcaaaactgc gtagtttttc gatagatcct ggtccgatgc ctcgtttggg cgaattaacg
    38881 atacgctcaa aactgattga atcttgagga tttaccagca cattcaaata cgctaaaata
    38941 tctttgattt ctttacgatc gtagaacttg tgccctccaa ccattttata aggaatattt
    39001 gctttcaaca gagtttcttc cattacacga gattgtgcgt tcgtccggta caagatggca
    39061 aagtcgccgt aattccgatg attgcttcga atctcttctt gcatccggtc tacgataaaa
    39121 cgggtttcgt ctcgttcatt atctgcacgg tagtaagtga tcttgtttcc ttctttgttt
    39181 tctgtccaca agttttttgg ttttcgattg ctattgtttt caatgacttg gtttgctgcg
    39241 ctaaggatgt ttttcgtgga gcgatagttt tgttctagca aaattactgc agcatctgga
    39301 taatcttttt caaaatccaa gatgttctgc atatctgcac cgcgccagcc gtaaatactt
    39361 tgatctgcat ctcccacgac gcacaggttc ctaaatctac ctgccaataa attgactagc
    39421 gtatactgtg cgtgatttgt atcttggtac tcatctacat gaatataatg gaatttgttt
    39481 tgataatacg tgagtgaatc aggatgttct tcaaataatc gaatcgtatt catgatcagg
    39541 tcgtcaaagt ccatacattg attatattga agctcttttt ggtaagcagc ataacatttt
    39601 gccgcaatct cttcgtaata actgccttgc atttttgcaa aatcctgagg ggtctgcagg
    39661 ctgttttttg cttgagagat tgttcctaat atacttcgag gatcatattt tttcggatca
    39721 atgttcagtt cttttagtat ccgtttcatc aaggtcagct gttcagaaga atcgatgatt
    39781 gtaaagttcc ggttataccc gataaaatca acatctcttc ttaaaatacg cacacacatc
    39841 gagtgaaaag tcgataccca gacatcttcg cctccagatg cgagaatcgc attgacacgt
    39901 tctttcattt cttttgcagc cttattggta aaagtaatcg ctaggatgtt ccaaggattg
    39961 acttcttttt cctcaatcaa ataggcaatg cggtgtgtca gcactctcgt ctttccactt
    40021 cctgcacctg ccattagcag taatggtccg tcagtgtgaa gaacagcttc tttctgacgg
    40081 gggttcatcc cgtttaataa ttcagctttg gatgacatta acaacatcct ttcttttatc
    40141 aatcttgtta attatagcat tttcttggct ttcattaaac tagctagttc aaaatcattt
    40201 gtttcttttt atggattttt ccttgtcaat tcgttgattt cgcgctaaat taaaagaaag
    40261 cgaggcgttt gaatggaaaa aaaaggaaaa atcaagaaat tagataagac tcagatatat
    40321 tttattctaa aaggaatatt tgttggactg ctgacaggaa tcgtcgttag cctgtttcga
    40381 ttatctatcg aaaagctaag cgatttagtt cgaagtatct atgaaatgag tcgggaacag
    40441 ccgatttact taattggcct agctatttgc tgcatccttg cagcattttt cgtgggttat
    40501 ctggtcaaaa atgaaccgga tatcaaggga agcgggattc cacaagtaga gggacagctt
    40561 cgcggggaat tatcaatgaa ctggttctct gttttatgga aaaagttcat tggcggtgta
    40621 ctttcagttg gtgcgggcct ttttctaggt agagaaggac cttctataca gcttggcgca
    40681 tccattggac aaggagctgg acaattgttt cggagtccat cttcagaaga aaagatttta
    40741 atttctagcg gtgcaagtag cggacttgca gcagcattca atgctcctat cgcaggatta
    40801 ctctttgttt tagaagaaat ccatcactcc ttttctccac ttgtttggct gacatcattt
    40861 gcttcagcaa tcactgctaa cttcgtctct ctttactttt tcggcttgag acctgtttta
    40921 tatctaggtg accttccttc tttgccgttg aaatattatg gttccttagt gatcttagga
    40981 gtacttctag gcatcttagg ttttatttat cagaatgtct tgctggcttt gccaaaattt
    41041 tacaaccgat tgcctttacc agcttatttt cacgggttcg ttcctttctt gctgattttg
    41101 ccaattggtt atttatggcc tagtactcta ggcggaggca atcaaatcgt tttagctttt
    41161 ggacatgctt ccttgccaat cattatgatt atcggtttgt ttgttcttcg attcgttttt
    41221 tccatgattt cttacggtgg gggtcttcca ggggggattt tccttccgat attgacattg
    41281 ggggctttaa tcggtagctt ttatggaaac attatggttg atttcgggat ggatccaatc
    41341 tacatcaagg attttgtcgt tattgccatg gctggttatt tcacagcgat tggaaaagca
    41401 ccgctgacag caattatttt agtgacagaa atggttggct ctcttaatca tctgatgcct
    41461 ttaggattgg ctgctcttgt tgcttatatc gtcaatgacc ttctcggcgg taatccaatt
    41521 tacgaatcat tattggaacg tctacttgga aatactcaga ccgcctcact aaaaggtgca
    41581 aggacaactt ttgattttcc agtaacggca gagagttctt tagacggatt aatggtacga
    41641 gacttcaatt ggcctaaaaa tatgctgcta atctctatac gcagaggaaa tcaggaaatc
    41701 ttgactcacg gtgatacgat catgaatgta ggagatattt tggttatctt gactgatgaa
    41761 gcaaacttgc caagaatcaa gcaggaaatc gaggctaaat cttctatcac aaatttgaag
    41821 caatcatttt aacgaacatg gaaaaactat tttacaacaa ctattcatct acactacttg
    41881 attctatatg attctataaa aaaagaagag gctgggaaaa cccagcctct tcttttttat
    41941 ttctatgctt ctgctactgc aggagctttt ctcaataaac cataaatcac accagatacg
    42001 actgctccga tagcaataaa caccaaatac atgaccgggt gactcagtaa gaatacaacg
    42061 aaaatcccgc cgtgaggagc cagtaatttg attccgaaag ctccgactaa tccgccagtt
    42121 aaagcagaac caataataaa acttggaatg gcacgaatcg ggtcagctgc tgcaaatgga
    42181 atcgatcctt ccgtgacaaa cgatagtccc atcacgatgt tcgtcaaacc agcatcttga
    42241 tctgttttgc tgaatttatt cttaaacaaa cgtgtagcaa cgaaaattgc caatggaggt
    42301 accattcctc cagccataac agaagccatc acgacacttc cgccagttgc aacagtcgta
    42361 gctaaagttg ctgtaccaaa cacataagcg gctttgttga ttggaccgcc taaatctgcc
    42421 gccatcattc cagctaataa tgcaccaagt aaagcagcat tcgaaccact catacttgtc
    42481 aagaaaccgt tcaatgcatc attgattgct ttcataggga cattgaccag caacatcaag
    42541 aaaccagtaa tcagtaagcc aaacactgga taaaagagga tcgttttaat tccatccaat
    42601 gattttggta agttcttgaa taatttacgt aaaaatacga tgacataacc agctaagaat
    42661 ccaccaatca gtgcacctaa gaatcctgca ccgcctgtat tagctaatgc accagcagca
    42721 aatccaacga tcaaaccagg gcggtcaccg atacttgaag caataaatcc tgctaatacc
    42781 ggcagcatga aaccaaatgc agcttgtccg atttggttga accagctagc agcttgattg
    42841 taattcccta gatttgataa ttgatcatgt ggcacgccaa taaattggtc gatcatgaaa
    42901 gaaagcgcaa tagcgattcc tccaccaata acgaaaggta acatgtgtga tacgccgttc
    42961 atcaaatctt tgtaaattct ttgtccgata gtaccatccg catttccttc ttctgaactg
    43021 ctttcttttc catcaccatg gaatactggt gcagtaccac taagggctag attgatcaac
    43081 tcttctggtt tacggatacc atcactgaca ggacgattga tcagttcttt tccgtcaaag
    43141 cgattcatct ctactttttt gtctgctgca acgattacac cttctgcacg ggcgatgtct
    43201 tcagcagtca atcggttttt gattccttct gaaccgttcg tttcgacttt gatctccaca
    43261 cccatttctt tcgctttttt cttcaaagca tcttctgcca tgtaagtgtg agcgatccct
    43321 gttggacaag ctgtaacggc aacaacgaat tttctttctg gatgagcaga agcttctgca
    43381 gcttgtgttt cttgttctgc tttttcttct gcttcttttt gctcttcagc tgtttggaac
    43441 aagtcttgga cttcttctgg tgtatttgct tcttttaatt ttcctacgaa atctgcatca
    43501 atcagcaatc gagatagtgc tgctaatgct tgcaaatgtg tgtcgtttgc gccttctgga
    43561 gctgcgatca tgaaaaacag ataagttggc tgtccgtcta aggcttcata atctactcct
    43621 ttttggcttt ttgcgaataa aactgttgct tctttaactg cactgttttt cgcgtgaggc
    43681 atggcgattc catcaccaag acctgtagaa gtttgtgctt cgcgggctag aattccttct
    43741 ttatatgttt cgatatcgga aatccggccg gcatcaaaca ttttttggac catttcatcg
    43801 attgcgcctt ttttatcagt cgcttgcaaa tccatgatca tcgcttcttt gaccattaaa
    43861 tccttgatat tcatgattat cctccttgaa actaatcaat ttttgtgatt tcaacttctg
    43921 gcagcaactc atcaatcaat tcgcgagctg ccaagtcatc agaaaaagct gttgcgcttc
    43981 cgcaagcaac tccccattta aaggcttcga ttggatcttt cgtacggctg taagccccaa
    44041 taaagccagc aatcattgaa tcccctgcac ctactgaatt tttcaatggg cgaactaata
    44101 cattcgtttt ataagttcct tgttccgtga agagcaaagc accgtctcca gccattgaga
    44161 tcagcacatt ctgtgcccct tcagctagta attttttccc atacggaatg atttcttcta
    44221 cagattggaa ggttgtgttg taaagctcag ctaattcgtg attgtttggt ttgatcaaca
    44281 acggtttctt aacaagtgca ttttttaggt catcacctgt tgtatcaatc acgaaatctg
    44341 cacctttttc tcgaatgata tcaatcagtt cttgataaaa tccttttcgc aggctagctg
    44401 gcgcgcttcc tgacaatacg acgatatctc cttgtcctac tgttcctaat gcttgcttca
    44461 actcatcgat ttcttcatct gaaatagatg ggcctaagcc attgatctca gtttcagtgt
    44521 ctgattttag tttgatattg attcttgtat cttcttttac ggttgtgaac aaagactgga
    44581 tttgttcttt ttcaagccac ttcgaaataa attcgccagt gaaccctcct aaaaacccta
    44641 gtgctgtaga ttgagaatcg atccgtttca aaatacggga aacattgatc ccttttcctc
    44701 caggtaactt gaagtcttcc ttcattcggt tcaaatcgcc tagttttaac ccatctacgc
    44761 gtacgatata atcaattgat ggattaagtg ttactgtgta aatcattgta ttgcctcctt
    44821 aattgttgtc ttttcacgga attcattaac gaattctaat ggacaacgct cggttatgat
    44881 cgtggctgct tccaaaggtg caacagaggt gaaggacaac tggttgaatt tggtatgatc
    44941 aatcaataca aatgtctctt cggaattttt catggctaaa cgtttaacag ctgcctcttc
    45001 tggatccgga gtcgtgaatc cttgttcaag atgggcaccg ttcattccca taaatgcttt
    45061 attaaaatga aaatttttta gttgttctat cgcattagat cctaaaacag cattagttgc
    45121 cagctttatc gttccaccta aaataatggt agcaagctcc aaatcaacta aatttgccgc
    45181 atgttgaacg gaatttgtca ctactgtgat ctgttttcct tgcagaaaag gaatcatttc
    45241 taacgtagta gagccagcgt ctaaataaat gacatcttcg tcattaatgg tttcagctgc
    45301 cgatttggcg atttgctgtt tttcgtgaac gtttttgagt gatttttcac tcatattctg
    45361 ctcaaaattc aataattgct ctttcttagc tccgccatga actctttcta gaaacccact
    45421 attctccagc tcttgtaaat cacgtcttat cgttgattca gaagcattca aaaggtttac
    45481 aagatcttgt gatttcacga ttttctgctg ttcaagcaag tctaggattt tttgtctgcg
    45541 ttcctctgta agcattttca cgatcacctc aatacaaata atagcatatt ttttttctac
    45601 tagcaatata aatcagtcaa aaacgttcat aaaaattata aatcagtcaa aaaaaaataa
    45661 aaaaccagtc agaaagactg gttttttatt tttttgtcta tcatcaagcc gttttatcgt
    45721 tttttatttt cgcttcttgt ttttttcata taaacagaag caggttttct ttccttatga
    45781 agaagataat aataaagtcc catcccgcca aacatgacca aaaaaccgat gactgatcga
    45841 ataggaaaat taggaacaaa aaagagacag acggataata atgatgccgg aaccattatt
    45901 cgataataga gttgtttatc cattttcttc acctcatatt ttttgtaaag taatcaattg
    45961 ttctaattct cggtaatcta aaaattgcac acgcagatgc tgctcacctt tcttgacatc
    46021 gaattggata cttcgccgct ttttctttat tcgtttgaag gtaacatcag agagtctatg
    46081 tttgatttcc tttaacggta attctgacag ggattctgaa ggaacataga ttcttaagga
    46141 taaaagatac tcttgatatt tttcatctgt tacctctact tctagatatt ccctaatata
    46201 ataacaataa gcttcgattc cattatccaa agcaacagtc ggatcgataa ctttatagca
    46261 catcatgcgt gatcgattct cgtcaaccca aggaaaatac gcctgagcat ttagtgttcc
    46321 gcgaacacgc ctttctttat aatctccttg ttggatatca taactttttg ttacaaaaaa
    46381 tgaagcattc tttttaggaa caggttgcat ttgtgcattc tgatactgga cagattggat
    46441 cgtatggtca gtgtaaatgt attcaacgaa tggcggtttc aataaaccga caccattgtc
    46501 ccaagcaaga tgatcaaacc caagctggtc cctaaaatag gattgaaaca tttgattgtt
    46561 tggcttttgc cagttaaagg ggtctcgata aagctggata catatgactt cgttttctaa
    46621 ttccttcact ttttctactt gttggatgtc gagaaaaatc ggtttaggga tgatttgatg
    46681 ataatagcga ctataataca tttcccaaca cgaagtgaaa cataaacctt ggtaaaaaag
    46741 atcgtatcct ggaaattggt tgcaatcgac tactacttcc ccgttttgtt gtttcatttt
    46801 aggtaaatgt tcgatttcat cctttttctc aaacaatagc cgttgagaga tctgttttgt
    46861 attgtgatag ataaactcgc tataagctcg catataggcg aacaccccat gagcaatcat
    46921 ttttttctga atgaagtctc gaatcaaaaa ttgcttttct ttgaaaacag agatagacaa
    46981 tacatttttt atcaataatt gattatcttc caagcgaaaa ctacattcgt tctcgtcatt
    47041 tcctacaact aaccaacttt cgtctcttac cgatgctagc attatttttt cttgaagctt
    47101 tcgaagatca tacggatccg cttgttgtgt gaaagagcgg ataaatgttg gctggatcaa
    47161 cagacgtgtc aaaatagccc aatcgcttat ttcctgtttt ttttcttctt catttaagtc
    47221 tgtgagctgg atagtcatca ataatttatc ttcgccaatc attttctcac ctctttcaaa
    47281 tctttttata atttaattat aaccaatgat aaagaaaaac taaaacttta tgtcttagaa
    47341 accaatttgg acgattacat gatagactaa gtaaaaagaa acaaagaggt gtttacaatg
    47401 gggttgaaat ttgaacgtac agataattta gataaacttt tcgaatcatt tgctgtcgat
    47461 ccggaaaaaa cagttgcacc aaaagaagag atcgatcgat ttctaaaacc acagccacaa
    47521 aatgaaaaga aaaaagacaa gaaacaaaag taagttaagc tcaaaaaaag acttaccagc
    47581 caaactttta tgaaagagtt tgactggtaa gtctttttta ttctttttat gattcaattg
    47641 cttgttcgat tgctgcccac gcttcttctt ttccctgttt tgtaacggaa gaaaagagaa
    47701 taaacgtgtc attgcgatca aaatccaacg cttttttgat ttcgctctca tgcttgttcc
    47761 atttaccacg agggatctta tctgccttag tggcaacaac aattacaggg atatcataat
    47821 acttcaaaaa ttgatacatc tggatatctt cttgagaagg cttatgacgc aaatctacta
    47881 aagaaacgac cgcacgtagc tggtctctag tcgttaaata tgtctcgatc atctttcccc
    47941 attttgctcg ctcagttttg gatacttttg catacccata tcccggaaca tcgacaaaat
    48001 gtaatgcgtt ctcgatcaaa taaaaattca atgtctgggt ctttcctggc ttaccggaag
    48061 tacgggctaa attctttcga tcaatcaatg tgttgataaa agaagatttc cctacatttg
    48121 atcgtcctgc cagagctatt tctggcaatt gtgtatctgg atattgtttt ggtgccactg
    48181 cacttatgat aatttcagct tgatggactt tcatggtctc tctccttaac aaggtgatga
    48241 ataaagcaag cggctgtcgc aaaagccaaa atgactttta cggcagcctc ttttcatcaa
    48301 cccgcttttt tctctgaatc tttatagatt actgttggct caccagacaa ttcagctgct
    48361 tcttttgtga taatcacttt ttcgatgttt tcatttgaag ggatatcaaa catcacatcc
    48421 atcatgattt cttcaatgat ggaacgaagt cctcgagcac cggtattgcg ttcgattgct
    48481 tttttcgcaa tggctctcaa cgcttcaggt tcgaattcaa gttcggtatt atctagagaa
    48541 agaagttttt gatattgttt gactagcgca tttttcggct cagtcaagat ccgtaccaga
    48601 tcatctgttg tcaatttctc taaagctgcc ataacaggta atcgtccgat aaactcaggg
    48661 atcaatccga attttagtaa atcttcagga atgatatgct gcatgacact ttcgtcttca
    48721 gaaagttttt ggttttgtgt accaaatcca atcgtctttt cgcccatacg attcttcaca
    48781 atcgtttcga tgccatcaaa tgcaccacct acgatgaaca atatgttcgt cgtatcaatc
    48841 tgaataaatt cttgatgtgg atgtttacgt ccgccctgag gtggcacact tgcaacagtt
    48901 ccttctagaa ttttcagcaa ggcttgttgg acaccttcgc cggaaacgtc acgagtaata
    48961 gatacatttt cgcttttacg cgcaatttta tcgatctcat caatatagat gatccctttt
    49021 tctgcaagtt ccacattgta atcagcagat tgaagcaatt taagcaaaat gttttctaca
    49081 tcttccccca cataaccagc ttctgtcaaa cttgtcgcat ctgcgattgc aaaaggtaca
    49141 ttcaatgttt ttgctaatgt ttgtgctaaa aatgttttac cagaccctgt aggtccgatc
    49201 aagcagatat tgcttttttg cagttcaaca tcatctgtgt tttcttgtgc ttgctgattg
    49261 acacgtttat agtgattgta tacggcaaca gctaacgagc gttttgcgcg atcttgtcca
    49321 atcacgtagt tattaaatac ttcaagaatt tctagtggtt tagggacttc agtgaattcg
    49381 cgtacagctt cttcataaaa ttcttcatca atgatttctt tacataggtc gatacattca
    49441 ttacagatgt agacccctgg accagctacg atctttttca cttcttcttg ggttttcccg
    49501 caaaaagaac agcgtacggt ctcattgcta cttggattgt catacatggt cttcacccct
    49561 taacttgcaa aaagtatgca agaaactatc aaagacttta cagtctttta ctatcataac
    49621 atagtttagt aaaaagaaaa agtgtttaac aaaggatcaa ctgacctata cctttgcatt
    49681 ttttactctt aacaaaaaat aaggaactgt gacaaaatcg tgtcacagtc ccttatttcg
    49741 aataaacaat tttctagaag tagcttcttc agaaatgatt tattacttga tttctattct
    49801 ttcgcttctg tcgctgtacc tgtgatcaat tcgattgctt ttttcattgt cacatcatgt
    49861 tccagcatat cttctgtcaa cacgcgtttg atttgttcga ttggcatgtt gtatgtttct
    49921 gataattctt ggatttctgc attgatttct tcttctgttg cttgaagatt ttcagcagct
    49981 gcaattgctt cgatcacaag atttgttctt gtgcgcattt ctgcttcacc ttcgaattgt
    50041 ttatgcaagt cttcttctgt agaaccagtt aattgatagt acatttcagg agagatacct
    50101 tgacgttgca tgttgttcaa gaattcgtcc attgaacgat gtacttcatc atgaaccatc
    50161 acgtgtggca attcaacgat ttctgcgttt tcaacagcca tacggatcgc tgcttcatct
    50221 tttgcatcgt ccgctgcttt ttcttttgtt tctactaatt ctttgcgata tttttctttc
    50281 aattcgtcta atgttgatac ttcgtcatca acatcttttg cgaattcgtc atctaattca
    50341 ggcacttctt ttgcttttac ttcatggatt gtcactttga agactgcttc ttttccagca
    50401 agatcttctg cttgataatc ttctgggaaa gttacattta catctagact ttcacctgat
    50461 ttatgaccaa ctaattgatc ttcaaaacct ggaatgaatg agcctgaacc taattcaagt
    50521 gagtaatttt cgccttttcc gccatcaaac gcttcgccat ctacaaagcc ttcgaagtcg
    50581 attactactg tatcgccatt ttcagctgca gcatcttctt tgataactaa ttcagcttgt
    50641 gcttcttgtt cacgtttgat acgtgcatcc acgtcagcat ctgttacttc acggtcttgt
    50701 ttttcaacaa ctagatcttt atattgtcct aattttactt ctggtttcac tgttacttca
    50761 gcagtgatta cccagtcttg acctttttcc atgctttcaa catcgatttt tggttgagaa
    50821 acaggatcga tgccagcttc tttgacagca gcttcataag cttctggtaa tacagcattc
    50881 aatgcatctt catataaagc ttcttcaccg tacatacggt tgaatacttg acgagaaact
    50941 ttccctttac ggaatccagg tacgttcaag ctttttttca ctttattaaa cgcagttgtt
    51001 aatccttttt ggatttcgtc ttgcgcaatc gtaaatttta aaacaccatc attcgtgcct
    51061 tttttttcaa atgttgcagt catttaattc cctccgagtt tagaaattca ttttatacat
    51121 caaaaacgtt tcatgcatac ctttaaatag tacactaacc ctttcaaatt gtaaacatat
    51181 tgaatggata gaatccttgt ttttcataac atttttatct ttttcactga taaaatctaa
    51241 tggaagaaga tcatttgctg caaaattttt tctctttttt ttaaaaaatc ttgtttttca
    51301 tcttctgtac cttcatacat cgtttttcct gtacacgcct ctacagtcag ttgaaaccag
    51361 cgcttcgggt cttcgatgta tgtatcatag acaggataaa caattgcaga ttgcaacgtg
    51421 aattcttctt ttaacacgga actaagcagc gcatcttcat tcccgtaacg atctgaaaat
    51481 acacataatt gctggtaact tgcctgctgc tgcggctttg gcagatcttt tggtatgact
    51541 ttgactaact tttcttcaat cgtcaaatag aaaacaggat ggcattcgtt caattgccta
    51601 agtgtctcca gtaattttgc ccttgctaaa atatggacct ctggcgatac aataagttta
    51661 ctcgctgttt ggataaactc atgataagga agctgttcga tctcatcaat agacgcaagc
    51721 tgacgtgcag gttccaacga aggaagttcc tccgatttat tgtggagtgc cttgatcttc
    51781 tgcctctcca tttgtccgga taattcttct gcaaattgga ttttttcaat gacccgctgc
    51841 tcgaaagctt cataaaaatc tgtacttgct atcaattttc tagcatataa aaaatcttgt
    51901 gtactgatta acaagtcaaa ataaaattca gcaaactcct cattttgaag atactgtttt
    51961 tcatgcaaga gtgcttgctt caatgcttct tttttctcgt ccaactcgta aagacaaaaa
    52021 acaagcaggc tattagcctc aaagctttct ttcaactcgt aagctcgttg aaataattct
    52081 tttgcctgct gatattgctg gtttgctagt gcttgttgtg ccactcgcat taaattctca
    52141 tatttttctg gaaaatcaat cggcttcacg cagtgtcctc ctcttgctca tattcagctc
    52201 atgttcaata gtagcaaaac aaaaaaagca tgacaattaa aaataactga agcaccctct
    52261 aaaagctaga ttttagatct gacttttaga aggtgctttc aactgttact gctttttatt
    52321 gctcatcagc tgttgacttc ctggttgaac gctttgatca gattgatcgt aacaacactt
    52381 gtattagtga aaaaatcgcc cagatgttgc tttttctgat taaagactgc tggaagatac
    52441 cctaacatca gcaaaggaaa ttttagaatg aaccggcaag caccttctct aactaaaacg
    52501 gttgaccagc taagttcttc ttcatcaaag ctgatgactc gaataccaaa gatcattttc
    52561 ccaatcgttt ggccatgatt gaatttcgtc aataaaataa aatacgctag ataaataagt
    52621 aaggctgtga atccgtagag actcagataa gaatctgaaa aattaagcca acctagattt
    52681 ttggcaatac caagtgttaa acgcgtaata gctccgatac acaaaagatc gaccaagaag
    52741 gcaaacattc ggataaaaaa acctgcatag aaatacttag gaaaatcatt tgcttttggc
    52801 ggttcatttc ttgtgtccgt atactttttc catttacgtt gtttcttttt gatttcttcg
    52861 ggcgtcaaag gctctctttc aaaagagctc agtacctttt gggaaaagtt ggagtcaggt
    52921 cctacagcag gctgctgttg cttatctttt tcttgttcct tattttcatt tgaaagctct
    52981 gacatgctta ctcacctccg taataataca ttgcttttgg tgattccgct gtccctagat
    53041 tctcaagaac ggataagatc tgactagtct cagaaggctt caatccctgg aattctgcta
    53101 gtttacttcc taaccatgta ctagcaaacc cagatggcgt ggaagaatat tctactagtt
    53161 ccgcatcttc aagctgttgt tcctttcgta tagcttttaa agcatcttca ggaaatccca
    53221 attcatcgac taaacctact tcttttgctt ggacaccatc ataaatcctt ccatcagcaa
    53281 tttttttgac ttcttcttca gatttattcc gtccctcgct gacgattgct acaaaacgac
    53341 tatatgcgct gtcgatatat gcttgaagca ccgcttcttc ttcttttgtc tctggtcgcg
    53401 ttcctgaacc catatctttc aacgctccgc ttttaactgt tgtatcttcc acacccagtt
    53461 tttctagaag tcctgaataa ttcaatcctg acatgatcac accaattgag ccggtcactg
    53521 tttcttctgt tgcaaagatt ttatctgctt gagcagaaat atagtagcca ccgctagctg
    53581 ccatgttttt catgctgaca tacattggga tatcatgttc tttacgaatc gtatccaatt
    53641 tcttagcgat ttccgcactt tcgtagacac cgccaccagg agaatttact tctaaaaaga
    53701 tacctttgac tgttggatct tcttcaatcg ccttcagttg ttccataaaa tttttatgat
    53761 tgtaaccttc agaagcaaat aaacctgaag accctcccgc agcaatcgtt ccatctactg
    53821 ataattttac gattttttgc gtactgtctc cctcttccaa cacatttgga gctagttctt
    53881 catccccata taaccaactg ttcaatccct caatttgatc gttttcctga ggcttagtca
    53941 aactagctga aactaacgaa accacaaata atcctgcagc aatcagaact gctgcccaac
    54001 gtcttttatt cattctttct cctttctaat acaagttttt tatgacgatc acagtttctg
    54061 actgctcaga agtgtcgaac cgctctgctt tttcttcccg cgcatacccg gaaaacgact
    54121 cttttgttaa ttcatcttct accgtaaact cacgagcttg agcaacttcc aaggcatcag
    54181 gtgcgatttc ttggatgcta tcccagccag tatctaaaaa tattttcgta tcatctgata
    54241 caccagctga tgcttcgaat ttggacaatt gtttttttaa cattcccagt gtcatgatct
    54301 caatttttga atccattttt actcctcctc tatccgatta ctttctattg taccgaactc
    54361 ctgaaaaaga tgatagataa aaacccatta ttttttgaca taaaaaggac atttgacaaa
    54421 gaatttgata tataccaatt tattttttat tctttttttg ttgacaagaa cattcgcaaa
    54481 aggttatgat tttaaatgtt tccagataaa aaacaaaaag gaggatagtt atacctttta
    54541 ggatagcgcc aggtctgtgt aaacagatga cgaggagagg gtttatcgaa agattcggcg
    54601 gatggcccta gggactgcac tctacagaaa atccacaaaa aatgtctgca aagataaaac
    54661 aaaaggattt tcctgcagct ggaagcattc atacgaattc attatagaaa gtaggatgtt
    54721 ttttatgtgc ggaattgttg gaatgattgg attaaaaaat gtaacaccag gattgattga
    54781 cggtctagaa aaattagaat accgtggtta tgattcagct ggtatctttg tctcagatgg
    54841 cacaactgat tatctggtca aagcacaagg cagaattcaa aatttgaaaa acaaaataaa
    54901 tattgatact acaggaaata taggtattgg tcatacacga tgggcaactc acgggcaacc
    54961 ttctgaagaa aacgcccatc cacatacttc ccaaagtgga cgcttcgtgc tcgttcacaa
    55021 cggcgtaatc gaaaactttg aagaacttaa aattgcttat ttagcaaatg accactttat
    55081 tggtgagacc gatactgaga ttatcgctca tctaattgaa actttcgcga aggatactac
    55141 aacgcaagag gcgtttttaa aagctttgcg ggtcatcaaa ggttcttacg ctttcgcttt
    55201 gattgatcgg acagcacctg atgttattta cgtcgctaaa aataaaagtc cactattaat
    55261 cggactcggc gatggattca atgtgattgc cagcgatgca atggctatgc ttgctcatac
    55321 gaaggaattc gttgagattg aagatgaaga gatggttacc gtcacatcag aaaaagttat
    55381 tattcaaaat tttgcaggtg acatcgtaga acgttcttca ttcgaagcac aagtagatgc
    55441 ttcagatatc gagaaaggta cctatccttt ttatatgctg aaagaaatcg acgaacagcc
    55501 tattatcatg cgtcgtatcg ctcaaaaata cgtaacagaa gacaatcgtg tctctcttga
    55561 tcaaaagctg gtcgatacat tgtctgatag cgatcgaatc tatatcgttg cttgcggaac
    55621 tagttaccat gcggggttag caggtaaaca gactttggaa aaattgacac agattcctgt
    55681 tgaggttcat ttagccagtg aatttggcta taacacacct cttctttcaa aaaaaccatt
    55741 ctttattttc ttgagtcaaa gtggcgaaac agcagacagc cgccaagtgc tggtaaaaat
    55801 caaccgtctc ggctaccctt cacttacgat tacaaatgtt gctggttcta ctctttcgcg
    55861 ggaagcatcc tttacactgc tattgcatgc tggacccgaa atcgcagtag catcaacgaa
    55921 agcttatact gcacaaatag ctgtactaac tttattagcc aaagctatcg gtgataaaaa
    55981 agaatttgaa acttctcttg tattcgatat cactcatgaa ttgagtttag ttgctaatgc
    56041 gatggaatcg gtcatcgccc aaaaagatta tttagaagag ttggcagaag tatatctcag
    56101 cgatacaaga aatgcctttt acattggacg tggtgctgac tacaacgttt ctcttgaagc
    56161 tgctttgaaa ttgaaagaaa tcagttatat ccaggcagaa ggttttgccg ctggcgaatt
    56221 gaaacatgga acgattgctt tgatcgaaga aggaacacca gtgatcggaa tcatcagtga
    56281 agaagtgact ggtgcgcata ctcgaggaaa cctgaaagaa gtcgaaagcc gtggcgctaa
    56341 aacactagtt attgtctcag agggattaga aaaggaaagt gaccagctgg tgctaccaac
    56401 tgtccaccct tatctaacta ctctaaccac agtcgtaccg actcaattac ttgcatatta
    56461 cgcaacgcta ttacggggat tcgacgttga caaaccacgt aatttagcga aatcagtgac
    56521 agtagaataa taaaagaaca aggaccagtg tcagacatct gacactggtc cttgttcttt
    56581 tattttttat gaaaatatct cttaatccag tccgatttct tctttgacta ctgccgcgat
    56641 tttatcgaca tagtaattaa ctttctcatc agtaggcgct tctgccatca ctcgaagtaa
    56701 tggttctgta ccggaaggac gaactaggat tcttccttct gatcccattt cagcttctgc
    56761 ctcttcaatc acttttttga ttgccggaac atccatcgca ccgtttttat cacttacgcg
    56821 gatattcact aatttttgcg gatagattgt tacctctgct gcaagctctg ataatttctt
    56881 acctgtctgt ttcatgatat tcagtaactg aattcctgaa agcatcccat ctccagttgt
    56941 gttaaaatct aagaaaatca tgtgcccaga ctgctcgccg ccaaagttat aatcgttttt
    57001 gcgcatttct tctacaacgt aacgatcacc aacttgtgta acgacatctt tcaaaccgat
    57061 tccttccacc gctttgtgga atcctaagtt actcatgaca gtcgtaacga tcgtatcttt
    57121 tttcaatcga tttttttctg ccaagtattt agcacagata aacatgattt tatcaccgtc
    57181 gacaatgttt ccaagttcat ccaccgcaat aatacgatcg ccatcgccat cgaaagctag
    57241 tccagcatct gctccttttt caacgaccat ctcagctaaa cgttctggat gggtagagcc
    57301 tacgccgtca ttgatattta acccgtctgg atttgtgccc attgtataaa aatctgtttc
    57361 aagatcggca aacaaacgat taacacttgt tgaagttgct ccgtttgctg catcgacaca
    57421 aatggtcaaa ccggaaaggt caccgttgat cgtttgtaca aggaattgag aatatttcaa
    57481 taagccttct ggaaattctt ctacagttcc caatccttca gctgatggac gaggcaattc
    57541 atccacttca gcatctaata atgcttcaat ttctagctct tggtcatcta ctagtttaaa
    57601 accatctgca ccgaaaaatt tgattccatt atcttgtgct ggattatgag aagcagaaat
    57661 catgacccct gcgcttgctt tttgcaaacg tgtcagataa gcaacacctg gtgtagagat
    57721 cacacctagc tggaacactt cgatacccac cgataaaagt cctgaaatca gcgcttcttc
    57781 taacatttga cctgagattc ttgtatcccg ccctactaac acgcgtgggc gttttccttc
    57841 ttcttcatgc tggcttagga cataacctcc gcaccgtccc agcttaaatg caagttctgg
    57901 cgtaagttcc ttgttggctt caccgcgtac gccgtctgtt ccaaaatatt tacccatagt
    57961 tgtaaaatta ctcctttaac ttaattttct gaggaatttg ttctgctcat ttgtgttgat
    58021 tcgcttgttt ctgatgtttc ttctgtactt gtggtcgagc tgcttgtccc agattccgaa
    58081 ctggaagaaa tggttgttgt cgatggttcc gttcctgaat cagcagatga aatacttcta
    58141 ctcgtacttg tattttcttg tgtccgactg ctcgtcgaag cagagctact gcttgacgtt
    58201 tgcaacacgg gtgtaatctc cacactaact gattcgggct tgatacttaa cccctcttct
    58261 actggtattt caattttacg agttgttcga tgattgatac ctgagacatt gatcggtaca
    58321 gcaatactgt caccgatcga tgcaagcaaa tcgctagagc cgcttacttc tgcttccagt
    58381 tttgaaaaac tgaaatcata gctttctacc ccggcagctg gtgttccttg ctgtatccca
    58441 taaagtcgta ccgacttgct tggcttgatc gaatcagctc tcactgttac ttgcactgga
    58501 tcagagacga tacttaatac ttcccctgct gcattcagcg cctgtattgg gacctcacta
    58561 ttaatcccat catctgtgac catcgaagcg tccacatcag ctactactcg tttgatttct
    58621 gccaatgttt tgtcacctgt agtgatagag acttttttag gactaacgga agtatcatca
    58681 agttcatatc cagatggtgt gaacgttgaa ggaatcaacg tttccacatc gaaatctttt
    58741 attacctttt tctcaaccgt tactgtaata gtcgatggtt cgatctttgc tgtgacagca
    58801 ctgctcaaat tttgaatttt tagcggaact tcatgtgttc cttcgcccag cttagtgata
    58861 tctgctgtga cgcggaacat ccgtgtatct tcgtttgcct ctgcattcaa ctgaactcgg
    58921 tttgcactgg aaagtttgac agtcactgca ttttcatatc catggatata gtagcgcttt
    58981 gaatcataag aaatcttgat cggcaccccc gtaactgttt gattatacgc ttcatttcca
    59041 gaaagcgtat ttggaacgga ctggccattt gcattgaagt acagcgcaat actgaacagc
    59101 aatgcaataa aactgtaaag aatgcttgtc cgtttctctt ttgagatcat ttgcgatccc
    59161 ctcccttagt cataccgtct aggaaagatt gaatcacgtt tttcttgttt tttgttttct
    59221 cttcagcaat tagttctcta cgcaagattg ccaaatattc ttgctgggat aagccagcca
    59281 tcagttcatt atctaacgtg atgctgacgt ccccggtttc ttcagagaca acgatggtaa
    59341 tcgcgtcact gacttcgctt ataccgacag ctgcccggtg tcgagtcccg aattctttag
    59401 ggatcaacat actttctgat agaggaagat aggcgcttgc tactgcgatc tttccttctt
    59461 ttacaataac tgcaccgtca tgcagcggtg tattaggaat aaagatattg atcagcagct
    59521 ccccagtgat atctgcatct aaagcaatcc ctgtctcaat atactcatct aatccagtat
    59581 gacgttcgat agtgatcaac gcaccgattt ttcgcttgga catatattgg atggctttgt
    59641 caaaagacaa gatcatcttc tcatcttcct gctgttcgct ttttgattgt ttgaaaagcg
    59701 aactgcgtcc aagatgttcc agtcctctcc ggatttccgg ctgaaaaatc acgatagcag
    59761 ctatcacccc gtacgtaatg acttggttca tcagccaaga caaggtgtgc agaccaatga
    59821 tttccgctac gattcggata acaataaaaa tagctacacc tttcagaagc tgaatcgctt
    59881 ttgtgcctcg cagcagttgg atcagtttgt atacaagata ccagacaacc agaatatcaa
    59941 gtatgttcac caaaaaattc agagagaaca agtcaccgga aaacatttgc tgccaatagt
    60001 ccaaattaaa aagttccccg atttgaaatg acataaaaag cctctcttct cttttgagat
    60061 ttcctctcta gtatatcata tgttttaatt ttagaggctg atatgctcgt atttttcttg
    60121 agaaaaacgc ataattttca agaaaattgc aagtatgcgt tttatttttc tctgcaataa
    60181 atatttttaa acttcagaga attattcatt atattttttc tgttattttt cctttttttc
    60241 ttttgtagct tgttccattt ttttacgtcg ttctgctctg gcagctcttc tagcagctct
    60301 tctttcttct gcttcaaggt ccgtcgtttt ttcttttcta gtcttaacgg gtacatcgat
    60361 agattcctct ggttcaagtc gtttcttgat tttttctaat tctcccgtca gtgcagcata
    60421 gttgtaaaaa cgtgttttag cgtcgtggac agtcttttca aataaagctt ttgcaagatt
    60481 tggatttgcg ttatctagcg aggaaaagcg aacttgtttc ctcatgaaat caggcatagc
    60541 agagaaatca ggttttttgt agtccaaagt cattgggttc ttgttttttt cgaccattgt
    60601 agggttatat cgatataatg accaataacc agaagaaaca gcttcttttg cttctttaag
    60661 cgattctttc attccgccaa gcaagccatg tgtgatacaa ggcgtatagg caataatcag
    60721 agaaggacca ggatactttt ctgcttcttc aaatgccttg atcgtttgca tttgattggc
    60781 acctgaagca atttgcgcca cataaacatt tccatatgtc atcgccatca ttcctagatc
    60841 tttctttgaa gcatactttc cactagccga aaatttagcg attgccgaag ctggtgttgc
    60901 ttttgaggtt tgaccgccag tattggaata gacttcgtta tccaaaacaa ggatattcac
    60961 gtctgctccg ctggcaagta catgatcgat cccgccaaaa ccaatgtcgt aagcccatcc
    61021 gtctccacca atcatccatt ggctgttttt tacgaataga tcctttctct tgtatatatc
    61081 ctccagttca gggaaaattg atcgttcgct ttcgatcgca gcttgcagtt ttgctgctct
    61141 ttgctgcgtt ccttcactct caaacatgtg tactaaccag tcttccatca attctcgtag
    61201 ttcttctgat acttttggca atacttcttt gattgaactt gccaaagcct cgcgacgagt
    61261 ctgtgccgct agaagcatac cataaccaaa ttcagcatta tcttccaata aagaatttga
    61321 ccatgcaggc ccttgaccac attcattggt cgtataagga gaagcaggag aagcaccgcc
    61381 ccaaatagaa gaacagcctg tcgcattcgc gatcatcatt cgatcaccga acatttgtgt
    61441 caataatttt acataaggtg tttcgccaca gcctgagcaa gcgcccgaga attcaagaag
    61501 cggttgattg aattgtgtag acaagacagt attctttcct ttgactggat ttgctttttg
    61561 tcttaatgtc attgcaaaag cccaattcac agcttgttct ttctgttctt cataaggctt
    61621 catcactaaa gctttttctt ttgcgggaca agcctctaca cacagaccac aacctgtaca
    61681 gtcttccact gaaacttgga tacggtattt tacgccgtct gcgcctcgca tttctctcac
    61741 gataaatcct tctggcgctt cttccatttc ctcttcatct gctaaaaatg gacggatggc
    61801 agcatgagga caaacaaagg cacattcatt acacatcgta cagcgatcac taatccactc
    61861 aggtacttct aaagcgacgc cccgcttttc catagaagcc gttcctaaag gcatttcgcc
    61921 agttgtcatt ccatttgcta caagtgtacc aacagagagt tgatttcctt cctgtgcatt
    61981 cactggttca agtatttctt gtatataacc agtgttcctt ttattgattt tttcaggcat
    62041 ctccaacgtt ctccaatggt ctgggatcgc gacttgatga agcagttcgg tcgtccgatc
    62101 gattgcttgt atgttttttt ctacgatagc cattgattta tgtccatagc ttttcagggc
    62161 ttcttctttt agcagcggaa gcacctcatc aaaaggaatg atgtcagcta atttaaaaaa
    62221 agccgtttcc attgccgtat tgatccggcg tcccaaaccg acttcttggg ccaatctcat
    62281 ggcattgatc gtatagaatc taatgttatt ttcagccaag taacgtttta attttaaggg
    62341 cagatgcgtt tctaattgtt cgtcgctcca aagagtattc aataaaaaaa tgccacctgg
    62401 cttcaatcct ttgaccaagt catagctatg caggtaagct ggtgtgtgac aggcaacaag
    62461 gtccgcatgc tcgacaagat acgctgaacg tatcggcgta tctccaaaac gaagatgaga
    62521 gacagtcaga ccgccagatt ttttcgaatc ataataaaaa tagccttgcg catatttatc
    62581 ggtgtgatct ccaataatct tgatagctga tttatttgct ccaactgttc cgtcagatcc
    62641 aaatccccaa aacttggcct ggaatgtttg cggttctgtc aaatccagac tttcctttgg
    62701 ttccaaagat tgataagtca catcatccac gatccctatg gtaaaccgtt ttttgcgtat
    62761 gcttgggtct tttttcaatt catcaaaaac tgctgagatt tggtcgggag tcacatcttt
    62821 agaccccaat ccgtagcgac caccaataac agccgggcga agttcagaat catataacgc
    62881 actttgaacg tctaataaga gaggttctcc acctgcccct ggttctttac ttcgatcaag
    62941 aacagcaatt gtttttactg tatgaggcaa cttttgcacg aatgtttcca ctggaaaagg
    63001 acgataaaga tggatattca aaaaacctgt tttccgtccg tttgcattca agtaatcaat
    63061 cgtttgttca atcgtctgtg caactgatcc catagaaaca atgacttctt ccgcatcgtc
    63121 tgctccgtaa taagtcacca agtcataaga agttcctcgt aatcggttga tctctttcat
    63181 atatttttga acgatttcag gtaatttatc ataatagggg ttgatcgttt cccgttgttg
    63241 aaaatggatg tctggattct ggttcgtacc actaacagtc ggatgattgg gattcatccc
    63301 tcgtttacga aatgaagcta atttctcctg attgatcaga ggagctaatt cttcatattc
    63361 caaaacttcg attttttgaa tctcgtgact cgtacggaat ccatcaaaga aattcaaaaa
    63421 agggacttct ccttcaatag aagcaagatg ggcaacaggt gctaaatcca tcacttcttg
    63481 tacactgctt tcagctaaca tagcaaatcc tgtttgtctt gcagccatca cgtctccgtg
    63541 atctccgaaa atattcaacg catttgtcgt cactgcccga ctagcaacgt ggaaaacact
    63601 tggcagtagt tccccagcaa tcttgtacat attcggtatc atcaaaagca gcccttgtga
    63661 agcggtatat gtagttgtca gtgcaccagt tttcaatgat ccatggacca caccagctgc
    63721 tcctgcttcg gattgcattt ctactacttt tactggttga ccgaatacgt tttcttttcc
    63781 tgctgctgac cattgatcta ctaattcagc catggtcgaa ctgggtgtta ttggatatat
    63841 tgcagcaatc tcggtaaatg cataagaaat atatgcggct gccgtattgc catccattgt
    63901 tttcattttt cgcatgattc atcatccttg ttcatattct ctcttattat ctgttccatt
    63961 ataacgtaaa acatgccatt tttttctttt atttataaaa atattttaca tgtatctatt
    64021 tgaattggtc atctaatttt tagacaaaca gtctaaatcg ttgccttttt ttgtcagaca
    64081 tgtataaact ataagcgaaa gaggtgtttt aacatggata tatcagtgat tgatgcaacg
    64141 aaaacaaata cacaaaaagg aatcctttat ggttcatcga atgctcctaa aaaaatggtc
    64201 gaattcatca acttagcttg tccgtattgc cgacaatggt ttgaagaatc ttatgagcta
    64261 ttagaagaag cagtccaaag cgggcaattg cagcgagtga tcaaattgtt cgataaagaa
    64321 aaagaaagct tacagcgagg gaacgtgatg caccggtatc tcacaatcag cgatggccaa
    64381 aaagcgatca aagagatcaa acaaatattc gatactcaag acgagtggaa acatctttct
    64441 ctccaagaag tagcggattt tgcagtagat aaactaaagc tgacagaaca aaaagacgaa
    64501 cagctgagcc aagctgtcat caacgaagct gagcaagcac atattcgatt tgttccaact
    64561 gtcatcttgg gtaaagaaat ctttgatgaa tctatttcaa tagaggaatt aaaagaattg
    64621 atccaaacaa aataaaaaaa taaggagcgt gggacaaaag taaagaacac ttttgtctca
    64681 cgctctaaac acgtataaac ggcggaagca gaagcaactc cttcttattt cttggagtta
    64741 aacgcttctg tcccagcctc cttattccga ataaatggtg tccaaaagct tgcatctgcc
    64801 agattatctc atttcttatg acaagctaag caaattatct ccaccaaata atttattcat
    64861 atttttgact tgcgcatact aactaattct taaattttca tacaaaattt ctcaatttcc
    64921 cttaacgagt aatatttatg tacggtgatt ctcactagta tcccaatcac tttttaagtt
    64981 atcccgattt caattctact tcaaaaatag ttgcttttat gtatgctgtt agttgattag
    65041 atggtcattt gttgagctag gtcgtttatt ttaaacaagt tgtttcttta gcttatacct
    65101 atctcgtcta ccaaacgcaa gatggcaaac acaaatgcat acgcatctgt atttgccact
    65161 ttgattcgta taaagctaac aactattaac tcctctcagc taatagttgc tctctttttt
    65221 attcagattc atttttgctg atcacagcct agtatttcaa tggttttgaa cactaaacca
    65281 aattgttcca ttacaacatt cactggctga tgaaatggat tagtagattt cgattattca
    65341 aagcgatttt ctattggtgg atcgtgttcc caactaataa gacggattcc aaacctatcg
    65401 tacagatctt ctaaaccact tataaatttt gcacctattt caggtgtttt attgcgtgga
    65461 tattctattg ttacgccatt aaatggatac caattaggat taggctctct ttttttctgt
    65521 acatctccca tagaatagtt agcactttct gggacataat atattcttcg accatcctct
    65581 aattcatcta ataagatatt tcgtgatcgt ctatcacttg ctaaatgtag acgccacgtg
    65641 cgataccatg ccatgctacc tgcatctgca gaaaatccat caaataatgc aacaattgta
    65701 tcttctatca caatcctttc cccatctact tcatattcta atctaaaatc aaactcccct
    65761 cgggtaattt ctggtcttgg tggcttagga cccaatatta ttctacttaa aaccgcattc
    65821 gttcccagat gaaacacagc tataacaaca ataatgatta atattttgtt tctatttgat
    65881 aaatttctca ttattcgcac atcacctcta cattctcaca tattagttcg taatactcct
    65941 ttaatatcaa aatatctact ggtcgtaata atattcgcta tgaattccta ctactcgtat
    66001 aagaaaggcc aaatcatcag cttttacttg ctgatgattt ggcttgtttc tatttatcgt
    66061 aattgttttc taatcaccaa aattgttaac aatcggttca gatatttccc agttgattaa
    66121 ggtaattccg tattgtgtca aaacttcttg cgcttctccg aaattaagta cagttcttat
    66181 gtatccactt tctcctaata taaagtctac ctctctttta aaaccttcct cgagatttct
    66241 aagggctata tgtggtttta atgacattga ttgtacattt tcccccatat aatattgcgc
    66301 acttcctcga ctaaaataga ttcctagatc gtccccaaaa ggtagatctt ctctaccact
    66361 agctaatcta cttctccatc ttcgttctct tttcatagaa cctgcgctga actcacaccc
    66421 gttaaactca gctatgacag tatcttcaat tatgttaatc tgtccatcta gttcgtattc
    66481 aatcctaaat ggaaattctc cataggtgat ttccggttcg ggtattttag gtgagattaa
    66541 tgcaaagaaa ggaaatatta agcttggcaa catcaaaaaa actcctccta tgatcacaat
    66601 tcccgatgta atccaaaata taatttgtac aattctcatt gatccattgt attccatttt
    66661 attcccccta ttactattca tatccgccta tctatatcga tcttccaatg cagagataat
    66721 tgcttccata ccttgatttt ttactgagtt acctatgcga gatatcattc tccactaagg
    66781 tgcatcatat tgtctatcta acatgattaa ttctccaata ggtgtatact cctccaaaaa
    66841 caacatttaa tgcatctccc tcgacaacat aagctaccat gtttgctgta tatgtgtttg
    66901 agtctaattc ataagcagat aaatttactg tcgctggatt aaatataatc gcattcgtat
    66961 ttatagctac tgaatttgct gcttcagcac caccttttga atgtccgatc attgtgattt
    67021 caatagtagg atttacgtgg acagccctca ccgtttaccc tgaccattat agaaatcgct
    67081 tacttttttg aactacgcaa caaaggatac aatgctccta acttcactgt ctccattttt
    67141 gtccactaag gaatataaaa aataggcatt aattcattta attcggtcac aacacgttac
    67201 cttttaaatc attatactgt atctcataca ttattatcaa taaactatta taaaaatgca
    67261 aaaaatgctt tatcaatcta ttttgtaatt ggtcataata tcattgaaat cataccctgt
    67321 atcgattttt cctatgaatc tcgtctattt gtgattgacg aaaaagaggc aagacagaca
    67381 ctggaatggt aattttaccc actattttgc tccaactatt tgtaatcgta cccacttttg
    67441 aaataccgag atgatcattt taattgatat gctgattccc aagcaaaacc ccaagaaatt
    67501 aaatgctcta tcgcatctag cttcttgggt tttgcttata taaattccaa catttaaatt
    67561 ttttatgctg ttcggtctca gaaatgccgt ttttgcttat atcacaatat caagatgtca
    67621 aattattcgt gttgccgatg actttgatta atttttttca aattgtttat taagactaat
    67681 gctgtctttt tgcagatacc aatgttcgaa ctttttaaca ttatcatatg atcctgcaag
    67741 ttttaacttc ttaccattag atgttatcaa agttataatt aaccttgcct ttttcccatt
    67801 aggatctttt attttatgaa ttttctgcag gtaatctctc ctaatggcag ctatttttac
    67861 ctcatcagtg ctgataaacc attgatcatc cacatacata ctgcctgtta tgttgtgttt
    67921 ttccatttct tccgagaacg aaaagtttaa ggctcgttct tacttccaaa gttgaatgag
    67981 tccatggatt ggacttagtg tgagaattga gaacaacaat aagaatccat atcctcccgc
    68041 aaacatccct aaggctatac tgtctctcca tccataagta ggattagaag agaaaagagt
    68101 caccgtttgt aataatatga aaaggaataa tccagcacaa acaccaaaga taacaactcc
    68161 aaacattgaa tttttataca atgaccattt atttataggc ttttctttca ttttacttcc
    68221 ccctactgtt ctgttcacac ggaatgaatt caaagttcat aagccctcct ctttgaacat
    68281 atgaatacga cttgctaact atactttaaa aaagcttgtt ttaagcggtt ataggcgagt
    68341 tgtaatctct attcatctca ttagcaaata acttgccgaa aagtctactg aaattaaagc
    68401 agatagtgga agaattattt agccaggata gacactattt gtgaaacaca tgtctttact
    68461 tttgaaattc tatgatgaaa agccttggaa tacagtagct cttaattggt aatacttttt
    68521 ccattataaa taacctcttt tcattttata tttatcccct ctaaaaataa tatctaatct
    68581 aatattttaa acttatattt ccctcgctgt gctaatagag cattactaaa taaatagaaa
    68641 ctagaaaaaa gcattacgcc tgccagaata aaccaattat ttgt
//
LOCUS       DACDTH010000008        64060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDTH010000008 DACDTH010000000
VERSION     DACDTH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.008"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="IWK54_000585"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="IWK54_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9592732.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1628)
                     /gene="dhaK"
                     /locus_tag="IWK54_000586"
     CDS             complement(645..1628)
                     /gene="dhaK"
                     /locus_tag="IWK54_000586"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9592733.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="IWK54_000587"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="IWK54_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9592734.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="IWK54_000588"
     CDS             2179..2499
                     /locus_tag="IWK54_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9592735.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3480)
                     /locus_tag="IWK54_000589"
     CDS             complement(2611..3480)
                     /locus_tag="IWK54_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9592736.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(3576..4850)
                     /locus_tag="IWK54_000590"
     CDS             complement(3576..4850)
                     /locus_tag="IWK54_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9592737.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4876..5385)
                     /locus_tag="IWK54_000591"
     CDS             complement(4876..5385)
                     /locus_tag="IWK54_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592738.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5560..7695)
                     /locus_tag="IWK54_000592"
     CDS             complement(5560..7695)
                     /locus_tag="IWK54_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9592739.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7778..9433)
                     /locus_tag="IWK54_000593"
     CDS             complement(7778..9433)
                     /locus_tag="IWK54_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9592740.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9435..9749)
                     /locus_tag="IWK54_000594"
     CDS             complement(9435..9749)
                     /locus_tag="IWK54_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9592741.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9919..10179
                     /locus_tag="IWK54_000595"
     CDS             9919..10179
                     /locus_tag="IWK54_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9592742.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10193..10507
                     /locus_tag="IWK54_000596"
     CDS             10193..10507
                     /locus_tag="IWK54_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9592743.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11237..11791)
                     /locus_tag="IWK54_000597"
     CDS             complement(11237..11791)
                     /locus_tag="IWK54_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9592744.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11917..13845)
                     /locus_tag="IWK54_000598"
     CDS             complement(11917..13845)
                     /locus_tag="IWK54_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9592745.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(14151..14867)
                     /locus_tag="IWK54_000599"
     CDS             complement(14151..14867)
                     /locus_tag="IWK54_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9592746.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14868..16691)
                     /gene="glpO"
                     /locus_tag="IWK54_000600"
     CDS             complement(14868..16691)
                     /gene="glpO"
                     /locus_tag="IWK54_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9592747.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16705..18201)
                     /gene="glpK"
                     /locus_tag="IWK54_000601"
     CDS             complement(16705..18201)
                     /gene="glpK"
                     /locus_tag="IWK54_000601"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9592748.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18337..19797)
                     /locus_tag="IWK54_000602"
     CDS             complement(18337..19797)
                     /locus_tag="IWK54_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9592749.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19787..21082)
                     /locus_tag="IWK54_000603"
     CDS             complement(19787..21082)
                     /locus_tag="IWK54_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9592750.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21414..22091)
                     /locus_tag="IWK54_000604"
     CDS             complement(21414..22091)
                     /locus_tag="IWK54_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9592751.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22121..23212)
                     /locus_tag="IWK54_000605"
     CDS             complement(22121..23212)
                     /locus_tag="IWK54_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9592752.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(23313..23747)
                     /locus_tag="IWK54_000606"
     CDS             complement(23313..23747)
                     /locus_tag="IWK54_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9592753.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24331..25788)
                     /gene="ascB"
                     /locus_tag="IWK54_000607"
     CDS             complement(24331..25788)
                     /gene="ascB"
                     /locus_tag="IWK54_000607"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9592754.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25785..27680)
                     /locus_tag="IWK54_000608"
     CDS             complement(25785..27680)
                     /locus_tag="IWK54_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9592755.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27738..29513)
                     /locus_tag="IWK54_000609"
     CDS             complement(27738..29513)
                     /locus_tag="IWK54_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9592756.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29563..31059)
                     /locus_tag="IWK54_000610"
     CDS             complement(29563..31059)
                     /locus_tag="IWK54_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9592757.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31253..32875)
                     /locus_tag="IWK54_000611"
     CDS             complement(31253..32875)
                     /locus_tag="IWK54_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9592758.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33324..34637)
                     /locus_tag="IWK54_000612"
     CDS             complement(33324..34637)
                     /locus_tag="IWK54_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9592759.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34882..35106)
                     /locus_tag="IWK54_000613"
     CDS             complement(34882..35106)
                     /locus_tag="IWK54_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592760.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35126..35494)
                     /locus_tag="IWK54_000614"
     CDS             complement(35126..35494)
                     /locus_tag="IWK54_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9592761.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(35692..36402)
                     /locus_tag="IWK54_000615"
     CDS             complement(35692..36402)
                     /locus_tag="IWK54_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592762.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36420..36917)
                     /locus_tag="IWK54_000616"
     CDS             complement(36420..36917)
                     /locus_tag="IWK54_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9592763.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36954..37406)
                     /locus_tag="IWK54_000617"
     CDS             complement(36954..37406)
                     /locus_tag="IWK54_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9592764.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37541..38146)
                     /locus_tag="IWK54_000618"
     CDS             complement(37541..38146)
                     /locus_tag="IWK54_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9592765.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38596..39216)
                     /locus_tag="IWK54_000619"
     CDS             complement(38596..39216)
                     /locus_tag="IWK54_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9592766.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39218..40087)
                     /locus_tag="IWK54_000620"
     CDS             complement(39218..40087)
                     /locus_tag="IWK54_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9592767.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(40110..40517)
                     /locus_tag="IWK54_000621"
     CDS             complement(40110..40517)
                     /locus_tag="IWK54_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9592768.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40536..41825)
                     /locus_tag="IWK54_000622"
     CDS             complement(40536..41825)
                     /locus_tag="IWK54_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9592769.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(41852..42154)
                     /locus_tag="IWK54_000623"
     CDS             complement(41852..42154)
                     /locus_tag="IWK54_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9592770.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(42179..44272)
                     /locus_tag="IWK54_000624"
     CDS             complement(42179..44272)
                     /locus_tag="IWK54_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9592771.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(44761..45525)
                     /locus_tag="IWK54_000625"
     CDS             complement(44761..45525)
                     /locus_tag="IWK54_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9592772.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(45636..46505)
                     /gene="mdcB"
                     /locus_tag="IWK54_000626"
     CDS             complement(45636..46505)
                     /gene="mdcB"
                     /locus_tag="IWK54_000626"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9592773.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(46530..47606)
                     /locus_tag="IWK54_000627"
     CDS             complement(46530..47606)
                     /locus_tag="IWK54_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9592774.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(47630..48223)
                     /locus_tag="IWK54_000628"
     CDS             complement(47630..48223)
                     /locus_tag="IWK54_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9592775.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(48227..49903)
                     /gene="mdcD"
                     /locus_tag="IWK54_000629"
     CDS             complement(48227..49903)
                     /gene="mdcD"
                     /locus_tag="IWK54_000629"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9592776.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(49893..50192)
                     /locus_tag="IWK54_000630"
     CDS             complement(49893..50192)
                     /locus_tag="IWK54_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9592777.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(50294..51940)
                     /gene="mdcA"
                     /locus_tag="IWK54_000631"
     CDS             complement(50294..51940)
                     /gene="mdcA"
                     /locus_tag="IWK54_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9592778.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(51947..52858)
                     /locus_tag="IWK54_000632"
     CDS             complement(51947..52858)
                     /locus_tag="IWK54_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9592779.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            52978..53835
                     /locus_tag="IWK54_000633"
     CDS             52978..53835
                     /locus_tag="IWK54_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9592780.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(54022..54384)
                     /locus_tag="IWK54_000634"
     CDS             complement(54022..54384)
                     /locus_tag="IWK54_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592781.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            54730..56169
                     /locus_tag="IWK54_000635"
     CDS             54730..56169
                     /locus_tag="IWK54_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9592782.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(56176..58728)
                     /locus_tag="IWK54_000636"
     CDS             complement(56176..58728)
                     /locus_tag="IWK54_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9592783.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(58842..60173)
                     /locus_tag="IWK54_000637"
     CDS             complement(58842..60173)
                     /locus_tag="IWK54_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9592784.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(60139..61617)
                     /locus_tag="IWK54_000638"
     CDS             complement(60139..61617)
                     /locus_tag="IWK54_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9592785.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(61922..63757)
                     /gene="tdc"
                     /locus_tag="IWK54_000639"
     CDS             complement(61922..63757)
                     /gene="tdc"
                     /locus_tag="IWK54_000639"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9592786.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        18295 a        13739 c        10836 g        21190 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tccgcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaag atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tagttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctttgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatct tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa tacataatag acaaacaagt atctaagaat aaattacctg
     4861 tatgggtgat tgagatcatt tcagttccac cccattttct ttatacaaat ctgcaatgaa
     4921 tgtcgtatct agatttttct cttcggagac agaataaaaa cggaccgttt ctctctcttc
     4981 ttttgttcca aaacgttccg tatacacttc ttcaagtaat tcttgtcttt ttttcaattt
     5041 ttcatcttca tattcaaaag atttatttac ttttttgtat agatttcgat tgttccacct
     5101 agtcaataca aatgaggtaa tcaggaaact gaaaaaaatc aatacaaaaa agccgattaa
     5161 gaaatacagc attttctttt ctgataaaaa agcgtacaag gaaaaatcct tggctctttc
     5221 tggtctcgtt aatgggaaaa agacccatat aaaaggaaga acaacgatca tccatcctaa
     5281 aagagccact aacgtttgcc aaatctttaa ccagtaatta ccttttgaaa aatacttatc
     5341 tttcaccaaa attgatgcaa cttcttctct tgttactttt ttcatatcct cacctcacgt
     5401 ttttttgtta cgacgtagga gtttaccata catttcattt cttttcacat aaagtgttcc
     5461 taaatttttc ctaagaaaaa gaacaaaaca tttctcatca gcaaatgaaa aatattttgt
     5521 tctttttatc agttgttctt tttatttttt atctcttaat tatgttgctc tttccaatac
     5581 gacaatgctt ttcattcctc tagatatcgc cgtgtacatt tgtctgcgtc ttctttgctc
     5641 atctccatcg ttttcaaatg gatataagat tacatgatca aattccagtc cttttgctag
     5701 actcaaagga atgatttggc agttttctaa tccatatgaa ttttttttaa tcgattcttg
     5761 tagcgtttgg gtttcctctt ctgtttgggt gataactgcc gctttttctc cttttagaga
     5821 aaccaaaatc tcagctagta ttttcaacaa ttcttcagag gatcgaaaag agcggtattc
     5881 gggttctttt ccttgcggtc ggatcgatac gatgtcgatc ttctcttgat ccacagcata
     5941 ggttttgaaa agctctgtga ttgctccgct agaacggtaa ctatttctaa gatcataccg
     6001 atggatcggc agatggtagt cgtcgaaaca acgcataata tcagcaaaag tagtggaaga
     6061 attaaagatt gcctgattct cgtcacctac taaagtaaag tgagtttttg gaaataattc
     6121 gatcaataga cctaattgtg ccagggtata atcttgtact tcatcaatca atagatagcg
     6181 aaatgctggt acatttacct tttcaacaag taaatgacga atagctaata taatgactgc
     6241 ttcatcaaca gtacgtgtgg gttgccaagc ataagctgag ccatatcgtt tttcataaat
     6301 tctttcaaac aactgatgct catccaccca tgccatttct tcgatttgtc tggttatttt
     6361 tcgatatttt ttttgtaaaa gtttccttgc ataagctaca atactttgtt ccgaatcatc
     6421 agaaatcaat ttaccgaata attccaactg cctatcttct gtcaacgaga gtacctgatt
     6481 ctgcacagca ggactttttg cttgtttcaa aagatgattc ttccattcgc tcatcagtgc
     6541 ctttttcgtt gcttgaagcc tatcgatcaa tttaggatga gcaggaactt tctcatatat
     6601 tttttcgatc gtttttttag aaaggactgt ttttccattt ttagtaagtc cacggataaa
     6661 attcggatga tctaaaaaca aaggatctga ttgttttaga ttatcgataa actttttgct
     6721 acgcaaacgt tctgtctgct ccgatacatt ttctcccgtg atacgtttga aataagcctc
     6781 ttcaccttct atttcctctg ctgaaagctg tgaaaccagc tgtatgatgg ttagattcag
     6841 tggatttctt tcacctaatg atggtaggac attcgaaata tagtcgataa accgatgatt
     6901 aggtgataga atcagcaagt catcggatgt catcgtatct cgatataaat agagcagata
     6961 cgcaatacgc tgcatgatag cagaggtttt accacttcct gctacgccat ttaccaagat
     7021 attttgactt ttcacatccc gaatgattgt gttttgttcc gattggatcg tagcagtgat
     7081 agccttcatt tcattggtct catcttggcc taagacttct aataatacgt catcttgaat
     7141 agctacagac gtatcaaaaa acttgaccaa acgatctctt tccaaaagaa attgtcgtct
     7201 ttctttaata accgtatcga ttttattttg acgaaccgta taagaagatg gaccaatcat
     7261 gttattgtaa aaaagttcgg caattggtga acgccaatca taaacaagat tttcatctgc
     7321 atcatctgtg aaatttgctg taccgatata aaaggattct tttggctcat tatccagaaa
     7381 gtctacaacg acttttccaa agtagggact tttcaataaa cgttcgatct ttgctagctg
     7441 attttctgcg tttttctgtt tgatattcat ctgatcgatt tctctatttt tcatttcaat
     7501 catcgaaaaa gtatccaggt tatctaagta actgtcaaaa ttcaatcgaa gctcgccagc
     7561 catttctttc aacgactggt ttccttctat ttttgccgta tccatcgatt cccgcagttc
     7621 gtttttttta tcaattagct tttgatacac gttcttcaga tatgcttctt ctaactgttt
     7681 ttgtgtgttg accatacaca ttctcctctt tttttaaagt ggtggacccg attagtttac
     7741 ctcttttaag agtcgaatgc aagcttttta acgagtatca tttgttagga tatatcagtt
     7801 cttcaggtcg gacagtttga tataaagaaa atgcaccatc aaggtttttt acgtggtagc
     7861 cagactgctt caagattctt tccgctagat agctacgtaa tccgctatga cagctgacga
     7921 tatattcttg ttggctgtct aattcattta atcggtcccg aagttcattc aaagggatat
     7981 gtttggcttc cttgaatttg cccttttcca attcttctgc tgttcgcaca tctaataaaa
     8041 tttttccttt tgctagttct gtcggcaatt catgccattg tacggtttca ctaagccctt
     8101 ctgcaatgtt cattgctgca tatcctagca tgtttactgg atctttggct gagccaaatg
     8161 gcggtgcata cgtcaattct aattccggta aatcgaagat cgtcaaatgc cctttgattg
     8221 ctgttgctaa aatatcgatc cgtttatcca caccttttgc cccaacacct tgtgcaccat
     8281 aaatctcgcc agtctctgga tggaagatca acttcagtaa aatatctgtt gcttctggat
     8341 aatagcttgc atgatctttt cctgatacat gaatgacttc aaaagacagt tcgttttctt
     8401 tggctgttct ttcacttaaa ccggtggatg cggcagtcat tccaaacgtt cgtataattg
     8461 ctgttccaat accgccttga tttcttcgag aaattccgga aatcgtgtcc gcaacttgtc
     8521 taccttgacg attggctggt gaagcaagag aaataagtgc atcttttcca gtgatttgct
     8581 gttttacaac gatagcatcc cccaccgcat aaatatcagg aatattcgtt tgataacgtt
     8641 cgtctactag gatacccccg cgtatgccta attcgattcc agcggtcact gctagctggt
     8701 tatcaggacg aacaccaatg gataaaataa ctaaatcagc agggatggta cgcccatcat
     8761 ccagtcgcaa tcgtttcccg tggtcttcaa atccagcgac agcatgagaa gtaatcactt
     8821 ctactccttg atgagacaat tctttttcta taaaagctgc catttcttca tccagtgttg
     8881 gaagaatatg tggtgcttgt tcgatcacca tgacttctaa acctcttctt tttaggtttt
     8941 ctgccatttc caatccgatg aatcctgcac caacgatcac ggcacgcttt ggctgttttt
     9001 ctaatgcatg catcacttta tcgatatctg gtacatttct aatagaaaaa acattgtcgg
     9061 cttctgttat ccctggaatc gatggaacaa ctggttttgc accaggagat aaaaccaatg
     9121 catcgtactg ttcaatgtgt ttttgaccct catgtacgac ttctaccgtt ttgttttcgg
     9181 gaaagatggc tgtcacttca tgatttggac gcacatctaa acaaaaacga gcggataatg
     9241 attctggcgt ttggacaaga agattttccc gttcactgat ttctcccgaa agataataag
     9301 gcaagccgca atttgcaaaa gaaacatacg gtcctttttc aaaaacaacg atttctgcat
     9361 cttccattaa tcggcgaagc cgtgtagcag cagacattcc acctgctaca cctccgacaa
     9421 taacgatttt catcttattt ccctcctact gttcgatcaa accattgatt catgccacca
     9481 cgtacattca ctacatcata accagatttc tttaattctt tagctgcttg tttactcctc
     9541 atccctgatt gacaaatcac atagacggtc ttttctttgt ctccatcata tctgtttata
     9601 ctttgtaatg gaacattttt agctcctttt atatgcccgc cccggtattc tgcaggtgtt
     9661 ctaacatcaa gtaacgctgg attttccaaa agcttttcct ttaattctgg gactgttatc
     9721 gattttattg attgaaatag caaattcatt ttctgcacct catttacatt tttataaata
     9781 cctcatgggg tatattaaac aggagcttta ttaatatgtc aaccagaatg actcttcatt
     9841 tttttcacta ctttttatat ttgcataaaa taccgataag gggtattatc taattataaa
     9901 aatagaggag aatgtgttat ggagattgat aataaaaata tcctgaaccg tttgaaacgt
     9961 acagagggac aaattcgtgg tatccaaaaa atgatcgaag acgaaaaaga atgtatggat
    10021 gtcatcactc aattaagcgc tgttcgttct agtattgatc gcgtaatggg catgatcgtt
    10081 gcagataatc tgaaaaattg ctttgagaaa cctgatagta atccagaaga acaagcggct
    10141 aaactagaac aagcgatcaa gatgattatt aaaaagtaaa ggaatgattt caatgaaagt
    10201 cgttttccat attgatgaat tagaaaaatg gtcagaaaca gggaaaaacg taaaaaacct
    10261 gataaaagct tctccagaaa cgacgattgt cgtatctgta aatggcatcg cgattaccgg
    10321 ttatctagac tctgccaatg cagaattctt agatttgcaa ggagtcgttt ttcacgcttg
    10381 cgccaatgct atgcgtgcta atcatatttc agaaagttct ttacctgaac aagtaatcgt
    10441 tgtaccagca ggtgtccttg atttagttga actgcaatcc caaggttatg cttatatcaa
    10501 gccttagtca gtagaggttg cgacagaagt gatcagcttc aagaaatttc ggcttatttt
    10561 tcgaagaagc tacttctgga aaaccgtgaa tcaaaggttg tgaaaacgct gtcctgcatc
    10621 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcgtttgg ttgggcttat
    10681 taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgaaat cagcggtttg
    10741 acctaagtat tagagaaaaa aacgggaaaa tagctcctca tattttgccg ttttttgagc
    10801 taataccgaa ggtcagctga ttgaacaccg tttaaggaag agattgtgaa aaagtcgctt
    10861 agtttcgagt attaagaaaa attttgtgaa aatggatttc tacatttttg cataaatttg
    10921 gcttaatacc gagaaactgg cttttgaata ccgtttaagg aagaggttgt aaaatcagcg
    10981 gtttgacctg agtattagag aaacaaacgg aaaaatagct gctcatattt tgccgttttt
    11041 tgagctaata ccgaaggtca gctgattgaa caccgtttac caagaggttg tgagcctacc
    11101 atttaactcc gaatcacaag aaataatttc aaaaacaact tctcctgttt ttggtttggt
    11161 tggcttatta tcgagaagta gctactggga gtctatttat caaaaaaagg aatgcgacat
    11221 aggtcacacc ccttttttat tttctttgaa acatctttcg gtactcgaca gcatacaaga
    11281 caaaagattg taaaagaccg taagtgatga cggcaaaaat gactctcacc cacagcgttg
    11341 gtaattctgg gaaaagcagc aaaaggatca tgccaacata gatgacagca gtcgctactt
    11401 tgccatacca cttcgctcca cttaatttga tatttttaga tagcccgatt aatccagcta
    11461 caaacataaa gccttctttt atcaagaata gaccaaacaa cagccaaagc cacggtcgat
    11521 accatgccat agacaaaatc agaaaaagtt gtgtcaattt atctgcaaac ggatcaagca
    11581 ccttccctaa gtctgttacc tgattatatc tccgagcaat gaccccgtct agataatcag
    11641 acaaacctga gacaacaatg accgctcctg ctatataaaa tgactgtctc agcacaaaat
    11701 aaagataaat cggcagcaaa aaaatacgga atatcgataa aagattcgga atcgtttgta
    11761 tttctttacg ccagtctgtg cttaatttca ttttatcgcc ctacctcctc agcactcttt
    11821 tctttttatt ttagcttaca cggggaataa aaccaatgat ttcactgttt ttatttttct
    11881 ttacagccac tgatattatg agctaatttg agataatcac ttgctgtttt catcgtttac
    11941 tcttctttct tcctgtcgtt tgcgcatttc ttttactagt actttatgag ctttttttga
    12001 tcctcgctct aggtcacgct gccgtttacg tgttttataa tcgataaaca gactgtctaa
    12061 atcgtaacct gtcgggtaca gatcttctgc tgaacgcacc agacgaacac gctgacgagg
    12121 cacgtggatt atttttttgt ctaaatatac atcaatcgta tcttcaccta tatcttcata
    12181 aactaacgct tctttttggt attcattgac aaatatccga tcaccttttg caaagagctg
    12241 gcgttctttt ttattctcct tcctatttgt aactgctgtc ttaggaagaa attctttttt
    12301 ctgtgtccga taggcttttt ccgccaagta acgctctgct tgatggatca atttcatcga
    12361 catatccatt ttatgagcaa tccacaaagc ttggctttct cctgtttctc caacccgcaa
    12421 ctgatattta ggacgaagtg cttcccgatc gaaagccata gcagccggca caaaatcttc
    12481 atgatctccg gcgaatttct tgatttctcc ataatgggtc gtggcaatga tcaatgcccc
    12541 ttgctcatac atagattcca tgattgcgat cgctagtgca gccccttcat ttggctctgt
    12601 tccactgcca atctcatcta aaagtaccaa cgtattcctt tttatctttt tcaaaatagc
    12661 agcgatgttt tgcatatgac cagaaaatgt actcaacgca ttttctaaat tttggtggtc
    12721 accgacatcg acgaaaatct catcaaagac agcaagctct gtcccttctt ttgccggaac
    12781 ttgcataccg aacatcgcca tcaaagtcaa taaaccgact gtcttcaaca cgactgtttt
    12841 gcctccggca tttgctccgg tgataatcaa gcctcgatag tctttaccga tttcaacatc
    12901 caatggtacc gcatgctcag gtaaaaatgg atgtctgcct tgcttgataa tgatatgttc
    12961 cgatttattg attctaggtg tgcttccatt gatttctcgg ctaaattttg cccgagcaaa
    13021 gatgatatct aacaccgtga tcgtttcgat caataaatcg atcacttctt cgttttctgc
    13081 gatagcacca gtcaatgccg ccaggacttg atattcttca ctgatttctt cagcttttaa
    13141 caactgatag tgttcattca atttagaaat cacagttggt tcaataaaaa ccgtcgttct
    13201 tttatttgat tcatcgataa tcgttccatc tattttattt ttatagctgg ctttgatagg
    13261 aatcgtatag tattcgcctt tttgaacaat cattgcttct tggatcattt ctttgttttt
    13321 cggatgacgc aagaatttca gtaattttga ctggatttct ttttcgatta gttgtaattg
    13381 tttgcgtact ttacgcaaat tacgtgacgc gtcgtcactg actttattat tttttatttt
    13441 ctggtcaatc aactcttcta catttatcaa atcaggaaga tgtttgctat aagcaaacaa
    13501 caagggtgtc tggtattgat tcttatcaaa aaactttgtg atcatccgac tgcttctaag
    13561 aaaatccgca tattcgatca aatcggttgg ctgcagaatc aacccttttt tgacttgttc
    13621 tgtcagtgca tcgatcctcg gcaaccccat aaatggaaca tgctgactgc tttctaagat
    13681 caagcgtgct tctcttgttt cttcttgacg atccaagaca gtttgtaggt ttgtcgagac
    13741 cgtcatatcg ctgattcgtt ttttgctata gtttccaatt gctcttgctt ggatttcctt
    13801 tttgatccga tcaaattgta attgactaat catttcgttg tccatattct ctccttttct
    13861 tgaagagata cctgtcccaa aataaataag ggcctagaga taaataaaag gatagaacac
    13921 agcttaaaaa agtcatgtcc tatccttatt agaatacaaa gatattgttt tcctgacagg
    13981 taccgtttca taagcacacc aaataaactt tgtttattgg ctattcgact tactgattag
    14041 ataccagcta cactcacaaa acatacccca ttcttctagg tcttatttat tttccactta
    14101 actatagcaa gacacatttt ggttgtcaaa ctttagtatg ttttatagat ttacaccatt
    14161 aatgaataga cacctaccgc gataattgct ccgatcattg gacctgcgat tggtacccat
    14221 gaatatgccc aatctgaagt tcctttatta gctattggca ataccgcatg tgctaatcga
    14281 ggaccaaagt cccgagcagg gttgattgca tatcctgttg gccctcctaa tgaaagaccg
    14341 atggctaaga tcaatccacc tacagcgaat acgttcgttc caggtgcaaa ttcgttttgt
    14401 ccaaaagcta gcaacccaag aactaataca aatgtaccaa ttaattctgt gatcacattt
    14461 gctggataat tccgtatagc aggacctgtt gcaaacgttc ctaaaatcgc gctttcatct
    14521 ttcgttgcat tccaatgtgg caaataagaa agccaaacaa gaatggcccc tgcaaaagct
    14581 cctaaaactt gcgcaatgat gaaaggaacg acaagattcc aactaattgc ccctgtcatc
    14641 gccatagcta gagagactgc aggattcaaa tgagctgggc ccatgaatcc agaaatatag
    14701 acagccattg ttacagcgag tccccaacca aatgcgatga caatccagcc agaagcctgt
    14761 gctttacttt tgtttaaatt cacagccgca cacacaccat ctccaagcaa aactaggatc
    14821 agtgtcccaa taaactctcc catgatttga gtcatatcac tgttcattta tttctccccc
    14881 tttagttccc gcagatctga ttctgcaatc aatgcttcta aacgtttagt ctgttgcgcc
    14941 ttctcctctt ctgtccattc aaaatatcca gcaattgcat cgatcacagg ttgcttgatc
    15001 tcatcaagct gatctcgttc aaacaacaga tgatttgtac gacgaatgag ataatcgcct
    15061 ggggccaaaa ccatttcttc ttctaaacca taacgtaacc gagctgactc agcgagactc
    15121 aaaccaggat aaggtgccat ttcttttgct aattcaaaga tacgtcgggc attagtcccg
    15181 taaaaatcag cgatataagt agcatcttct tcctctaaac cggctgctac tccttctttt
    15241 gctaattctg tcactgtttc ttctaattta gttggatcga agtttccacc tgaaatctga
    15301 tattttttag agtcgatttc tttcgtctct attccgtatt cttcttttaa taattggcgt
    15361 atcaatcgca aagctccttc tgccatctta cggtaatcgg tgattttccc accagataaa
    15421 gtcagcaaac catctggttc tctttctaaa gaactacctc tggaaatcgt agaaggtgct
    15481 ttttcatcac ggctgttttc caaatggttc aacacgtctt ctacttcagc acgagaaact
    15541 ttgttttcct tatattcact tactgtatcg acaactttat tgaagctctt gtctgaaatc
    15601 gatccattat ctccaccatt gtaatcagaa ccagaattac caatcaacag tggacgaagt
    15661 cctgcccagc ttgcttcgat atcattcaat gtgatatttg cttctggata gcgatgattg
    15721 atcacatcca atagataatc tacatcttct tgtgtgactt tcggatccgt aaaatctcct
    15781 tggtaatccg tatctgtcgt accaaagtaa gtcttgtttt ctcttggaat agcaaaaacc
    15841 atccgcccat cttgctttcc tgtatcgaag tatgtgggtt gcggtacagg cagttttttc
    15901 gcatctacaa ctaaatggat ccctttagtt ggacgcattt taggagagac tggacgcgta
    15961 aaattcaagt ttcgtacttt atctacccaa ggaccactcg tattgatcac taattttgct
    16021 ttgatttcga tcacttcatc tgtcagcagg tcacgagctt tgacgccaac gatttgatcg
    16081 ccatcatata aaaagccaac cgctttcatt ttacttacta gataagcccc atcttcggca
    16141 gcctttttga tattatcaat cactaaacgg gcatcattgt tgcggaaatc tagatagaca
    16201 cccgcgcctt ttagcccttc ttttttcaaa aacggttctc tttccaatac ttcttctggt
    16261 gtcagcgtat aattctcata tttggttcct gtcacatttg ccagtttgtc ataaaggtcc
    16321 attgctactt tgaccgagaa catattgaag gttgttgctc cttcgccctc atagattggc
    16381 aaaagcattg gatctggttt tgggatgtgt ggggcaatac tttgtacgac tgcacgttca
    16441 ccaactgtgt cagctactac ttccacatca aatgtcttca gataacgaat accgccatga
    16501 acaagtttgg tcgagcgaga ggaagtccct tctgcaaaat cctgcatttc gatcaatcct
    16561 gttttgattc ctgatgctgc tgactggact gccacaccgg caccagtgat tccgccaccg
    16621 atgatcaaca gatccaattc ttcttgctgc attttttgaa tgctatcttg tcttgtcttg
    16681 tttgaaaaca tatggatttc ctccttattc aaatgcttgg gtagctttta ctgctttttt
    16741 ccaaccgttg taaagttttt ctcggcgctc ttcctccatt tgaacttcaa atagcttgcc
    16801 agcttcataa aattcgcgga tctcgtctgt atctttccaa taaccgacag ctaatcctgc
    16861 taaaaatgct gcccctagcg ccgttgtttc caagttttct gctcgttgga tcggtacatt
    16921 caagatatct gtttggaatt gcatcagata ttcattgttt gctgcaccgc catctacttt
    16981 caatacagga atcttgattc cagtgtcatc ttgcatcgta tcgatgatat ctctcacttg
    17041 ataagcaatc gactgtaaag tagctttaat gatatcttct tttgtcgtac cacgtgtcaa
    17101 gccaaacata gcccctcgtg ctgcctgatc ccaataagga gcacctagcc caacgaatgc
    17161 aggaacaaca taaacctcat cttcgcttgt agagcgtttc gctgcgtctt ctgaatcgct
    17221 agctttttgc aacatttgaa gtccatctct cagccattga atcgaagaac cagccacaaa
    17281 aatacttcct tctaaagcat agtaaacctt tccatttatg ccgtaaccaa ttgtcgtcaa
    17341 taaattattt ttggataatt gcggtttttc accggtattc atgacaataa atgaacctgt
    17401 accataagta ttcttgacca tgcctggttc gaatgccatt tgtccgaata atgcggcttg
    17461 ctggtctcct gccatccctg aaattggtgc ttcgcttcca taaaaatgat aaccttgcgt
    17521 ataaccataa acttctgagt tagaagtgac ttttggtaac atcactcgag gaatatttag
    17581 taaatctaaa atctcctgat cccaatctag ctgatggata ttgaacaaca tcgtccgact
    17641 ggcattcgag taatcagtaa catgtgcttg tccatcggtc agtttccata ctaaccaaga
    17701 atcgatcgtt ccaaacatca attcgccttt ttcagctcgt tcttgcgcgc cttctacatg
    17761 atcaagaatc cagcgtatct tcgttgctga gaaataggca tcgatcacta accctgtttt
    17821 ttcgtggaaa aattcttgat agccttcatt ttttaagtta tctgcaattc cagctgactg
    17881 tctggattgc caaacaactg catgatagat tggacgtcct gtatcttttt cccatattac
    17941 tgttgtttca cgctggttcg ttatgccaat cccagcaatc tgatttggtt tgatccctga
    18001 ttcgatgaac gctccggcaa taactgactg tacagaattc cagatttcat ttgcatcatg
    18061 ttccacccaa ctttcttgcg ggaaatattg cgtgaactct ttttgagaac tgccgatgtg
    18121 tcttcctttc ttgtcaaaga taattgctcg tgagctagtt gtaccttggt caatcgccat
    18181 gatatacgtt gattctgtca ttgtgattcc ctccattata tgcaaacgct ttctttaact
    18241 actcttatca tactactgtt ttaaaaaaag aaactgtcaa aattttattc tttttttctt
    18301 tttttgacag ttttttatta tgaagctatt ttttaatcaa ttcttttctt ttttcagcgt
    18361 ctctattctt cttttcaatc gaataatgtc gtaaggtgac gcaaattctg acagataata
    18421 ttcacactgt tttttgtttc tctggcaaaa tgtgatcacc aaatcatatt ctttaccttc
    18481 ttcataactt ccaatctcaa taccgccgat tcccctcagt tctctgatca gatattgttg
    18541 aaaagcaata cgataaatcg gcagactttc taaatcgatt ccaatcacta ctggttttgc
    18601 aatataaaaa tcgatcatca aaagaacatt ggcatactgg atcatcaggt aactaagctc
    18661 actatcttca gctcttttcc tcggcaattg ctgcaccgct gtttcctgta atttcgccag
    18721 taactgtgtt aaggaatgcc cgagcatttt ctgctgctgt tccaaaagcc gatcacgctc
    18781 atagacttcg atctttccaa caaagaaaaa ccattcattg ttgatctggg aaatttggta
    18841 gcctactgcc ttttcttctt tgattttcaa acgacgagga cgataatgcc ataacatcgt
    18901 ttcccgaatg taagtatcta aaacagcggt tggtaacttt ttgctccgtg tcaaatcata
    18961 ctgataaaat atttcttcgt ctactatcga aaaagaaaca aaaaagctgt aaaacatcat
    19021 tgtttcgtat acattcagtt ctgctgctgt tcgactaaga tgcatcacga tgattggatc
    19081 gattgcttga taaagccggt ccgactgata gagtaatttc ttttctttca aagttgcgta
    19141 cgtacttttt tcattcaata gtcttttttt cgtaatcccg agccagcaag caatctgtgc
    19201 ttctgctgat gccgtaaacg ttgtttctaa tacacggtcc aatcctagaa tgaacggtct
    19261 tttttctggt gtattttgca aaaaaagcgg tcgctgatct tccgggatat attggaatag
    19321 ttcataataa aaatatcgta tctgtagctc ttctccgtat aattgaccgt ttttgatttg
    19381 taatccaaat tcctctaaga gctggttcaa ttctttgatc tttcgaaaaa cagaagactc
    19441 actgatatta aacgctgtcg ctaatcgaac gatggatact tgctgatgtt ctaataaata
    19501 gacgagcatc ttaaacttca agctttcctg caataaaaac aatagaattt tttccaaact
    19561 tacatcttcg tccaacacaa gcagcgcctg atactcattt cgctgtactt ctactttttt
    19621 ttccatcatt cggccaatca attcaatatc ttccaaatat tgatcaaacg aggcttttga
    19681 cagttggaca tgctctctca aaccattgat agaggctgtt cccccttgca gaacaaggtt
    19741 tcgcaaaatc aggatctgta actgctcttt tctttctaat aatgcttcaa tcttcatagg
    19801 cagtattcgc cacctttgac cacaccatat ctggcacacg gtattctggc tggaaaaata
    19861 gctcatgttc ttctaactgt tcaaacgtcc atccttcttc tactgcaatt gtcagttggt
    19921 caagaaggtg gaacatctct tttttgcttt ggagctgtgc tccaagaatc tttttttcag
    19981 aatcaaatag aaatttgagt ttaactttgc tttgatttat tgctgtaagc ttgtagtcta
    20041 cgtatcctgt ttcaattttt tggggataaa aaatgctctc ttcttctgtc aaacctacgc
    20101 ttcctaagaa atagccaaac cactgattgc cgactggacg ataggttccc tgatcctttg
    20161 tctgtgagcc actaattgtc ttgctgacat ttcgtgctgt acggacagcg ttatttacta
    20221 gagaaacata cattttttca tttgttggac gaaaagttac ttggatcgca tcgccaatcg
    20281 cataaacgtc tttttgagac gtttgcaagt agtcatctac ccagatcgtt ccatcatcat
    20341 tcaatgttaa ttgttcttcc cacatctgat tgtcggggcg agaatgatta gccagcaaaa
    20401 caagatcata gttctccttt cttttttccg tctctacaat agcggtttca ttttcggtca
    20461 aagacacaac ttgttcttct aaaaaaagtg aaagcccttg atttttcatt tcctttacta
    20521 acggttctgt catttcagga tctagataac gaaaaagaat tgactttcgg ctttcgtaaa
    20581 gatggatttg tttttttctt gatgccaacc cttcagctat ttcgattcct gcttgtcctg
    20641 caccaataat tgcaattttc tttgcattca tgatcttttg ctgaagagcc gttacgtttt
    20701 gtatcttttt aactgggtga ataagcgttg attctgcaga aatatttcga aacttttgat
    20761 ttgaaccagt cgctagaatc aaacgatcaa aatccaattt ttttcctcct gctaaaacga
    20821 cttttttttc tttcaatcct attactgtct tttctgtgta aacatggatt cgatagcggt
    20881 cagtcagttc ttctttcgtg atccaagaaa gttgtttttc actggtaacc tttcctttta
    20941 atatcaaatt gaacccgctg ggaatccatc ctaacttcga ttgcttttca atcaaatata
    21001 tttcacttgt tggcatcaat tggcgaagat aaatagccgc atgaatccct gcaaacgacc
    21061 cgccgacaat tacaatgttc atctgcattc ctctttctta taaggtgttc tactgctaac
    21121 ttctcaatca gaaaacacag tattcataac tcgctttcaa ccttatgata catagcgatg
    21181 cagcagaatc aaccctctcc atcataccgt gttctaggtc tgcttctcaa taatgtacta
    21241 gtggacctaa ttgaagagta tcatttctga agagatgttt caaaaaatgt actcttataa
    21301 aagaaaaaaa ctcgcctttt acgtacctct gtccaaatag gaaacagaaa agaacgaaaa
    21361 gacgagtttt agcgtgtgta atgtaatagt tagaatagac acatcattta tttttaattc
    21421 tttaatgctt caacaactgc ttcgcctaat tgatgagcag attgaggatt ttgccctgta
    21481 agaagccgcc catcaatttg aaccacttct gaaaaagctg ctccagactg atagtttgct
    21541 cctttttctt tcaaagcatc ttctgctaag aatggtactt cattcgttgt accatttgct
    21601 tcctcttctt cattactgaa cccagtcact gtacgtccag ctaaaataga ttttccagac
    21661 tcatctgtaa ctggcaacaa tcctacaaca ccgtgacaaa cagctgagac cagcccttca
    21721 tttcgataga tttgtgaagc gactttccca agatctattg cttctggaaa atcccacatt
    21781 gttccgtggc caccagcata ataaaccgct gcataatcat ttgcatccac atcatcagga
    21841 cgtaaggtat ttcccaaggc ccgatttctg aaatcttcat cttcataata ggcccaatcg
    21901 atttcatcca tattcaaaac actttgcggt tctaaaggta catatccgcc tttaggactg
    21961 acaaaatctg catcgatccc attttttact aatacatcat aaaaatgagt cagttcactt
    22021 aaccacaaac ctgtaggacg ctcgatcttt tcgtactttt ctacatttgt taatacgatc
    22081 aatactttca ttttcccatc ctctttctat aaaaattgcg ttattgataa cgtgctttca
    22141 agaaattata taatgggctt ggcaaataaa gctctttata tgcttcttca aaaggtttcg
    22201 ttacaaattc atcctcacgg ttttccaata atttctctgc ccagtcagga tcgacgatca
    22261 tttgctgtcc aattgctgca agttctgcat atttcaatac ctgctctaca tcttcgcgat
    22321 tgcggatccc accgacacca actaaaggga tctttccttg aatcgtgtta tggaccactt
    22381 caagaatcga tttatcgttg tatttatcag aacgagctat tcgatcatac atatttaatg
    22441 aaacatgcag ataatctagt tttgactcac gtaatcgttc aagaagccag atcgtgtctt
    22501 caaaacggat acctggtgtt tcatattctt ctggtgagaa acgataccct acgatgaatg
    22561 gttttttagc atattgttca accgctgaga aaaccgttgt taataattct tcaataaaac
    22621 gataacgatt ttccaagcta ccgccccatt gatcctctcg tctattcgaa tgcggagaga
    22681 aaaattgttg gatcaaatac gtatttgctc catgcaactc gattccatca aatcctgatt
    22741 caatcgctct acgtgtggct ttgccaaatg cttggattgt ttcaacgatt tcttcttcac
    22801 tcattgctcg tggtgtttcc gcatcaggac gttttgctgc aactgcactt gcagatacag
    22861 gctgttctcc agataaagtt gctcgacttg tcattcgtcc accgtggaaa atctgaataa
    22921 tcgcttttgc gccttctcct tggattgctt tcgctagctc acttaatcga ggaatcattt
    22981 catcatgagc gatgctgagt tcacctggcc agccttttcc gatatcttgg acatttgcag
    23041 caccagtaat aacagcacca agacctttag aacgttgttt ataataagtg atctcatctt
    23101 cggtgatcac tccattgaaa aagctgagtt gtgtcgtcat aggcgacagc atcaatcggt
    23161 tcttcaattg aactcctgaa ggtaaagtaa ttgattgatc aaattgtgac attgtcattc
    23221 ctcccataat tcgctctgat taacctatcg agaatcatct atcattaaac cactcgatta
    23281 gttgtacatg caactaattt gcttaaaaaa ttttattttt tttgtaactt atctgttgca
    23341 tcattcattg tctgggctaa ttctttactt gcttcatttc ccaaaactgc tttgtatgac
    23401 tgatgaagtt cttcccagct accatcgact tccttagcaa attggcgtcc ttgttctgta
    23461 agataaacta atgacaagcg accttcgcta actgttttga cgtatccttt atattctaat
    23521 ttttcaacaa aacgagcaat cgttgatggg gcggaataga gcatttgtgc aatatctttt
    23581 tgtggtattc catctttttc ttttaatatc aataaaataa aaccataagt tggtgccaga
    23641 cctgtccttc taaacgcttc atcagctaat ttattcaaca tgcgatctag ctttttcacg
    23701 ctaaagaaaa gacattcatc taacgctttt tcaattggct tttccatttc atcccctcca
    23761 ttggcgtaca tatcatagta tgatttttat tttttgtcaa aaaacacgga ataatgtccg
    23821 cctcctctgt ctagatttga aaaaaagaag tagccatctt caaagaattt cggcttgttt
    23881 tcgacaaaac tccttctgga acaccgtgaa tcagaggttg tgaaaaagtc gtttagtttt
    23941 gagtatgaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttggcttaa
    24001 taccgaaaaa ctggcttttg aacaccgttt atatcagagg ttgtgaaaaa gccgtcctgc
    24061 atcaagtaat aagaacagcc aaacaaaaat agttcctcat attttgtcgt tttttgagct
    24121 aatgccgatg gtcagctgat tgtacaccgt ttattgagag gtcgtgagcc tgccgttgaa
    24181 ctccgagtca caaggagcaa tttcaaaaaa cagcttctca tgttttatga ctatggtgac
    24241 ttgtgatcga ggagttggca ggagaatact gtttattcgt aaagaagggg ctgtgacaag
    24301 acttttgtca cagtccctta tttcctttta ttataggatt tcgccatttg attggatgac
    24361 ttgtctgtac caatcaaatg attttttctt taaccgtttc tttgtgccat tcccttcgtc
    24421 atcttgatca acatagatat atccataacg ttttgacatt tgtgaagtag aagcgctgac
    24481 gatatcaatg cagccccacg acgtataacc aaataaatct actccatctt tgatagcttc
    24541 cttcatctgc tcaatatgct gtttcaaata gtcaatgcgg taatcgtctt gaacggttcc
    24601 atcttcttct actttgtcta tagctccgag tccgttttca gctatgaaca gtggtttttg
    24661 atacctatca tataattgat tcaaagcaat tctcaaaccc ttaggatcaa tctgccagcc
    24721 ccattcactt gatggaagat acggattttt aattcctcca gttattaagt ttccttggac
    24781 cccttcccaa ttttctggat gagcactgac tgcactagac atgtagtagc taaaagaaac
    24841 aaaatcaaca gggtaggctg ctaagatttc ttcatcacct acttctattt ttaagttgac
    24901 acctttttct tcaaacattc gttgcgtata cgctggataa tatcctcttg cttgaacatc
    24961 actaaagaac aaagaagttc tttgctgctc aattgttttt tgaatatcat ccggatcaca
    25021 agaatacgga acaagaagct gatagctgat catacaaccg acttgtgaac ctttgataat
    25081 ttcatgacaa gcttttgtag ctaaagcact tgctataaac tgatggtgtg caccttggta
    25141 tttcgcttgt tccaaatggg gatggttttc ttcaattact cctacgctaa caaaagggtg
    25201 atgttttaca cagttgatct cattgaaagt catccaatat tttactttat ttttatagcg
    25261 tttgaatact gtttcagcga aacgaacgta aaactgaata agctttcgat taggccatcc
    25321 accatattct gtaaccaaat ataacggcat ttcataatgg gaaagagaaa cgactggctc
    25381 aatcccatat tttagacatt cgtcaaacac ttgatcataa aaaaataatc ccgcttcatt
    25441 gggtgtttct tcatcaccat gaggaaaaat tcttgtccat gcgatagaca tccgatagca
    25501 tttaaaaccc atttcagcaa ataaacggat gtcttcttta aagtgatgat agaaatcatt
    25561 tccgtgtctt ttaggataat aatacttgct gttgggatcc tgcgccgctt ttacttgctg
    25621 atgcgtcatc atatgccatt gatctgtcca tttttcttta ggtaaatcgc tgtcgaatgt
    25681 atacacatca gacacagata atcctttttc atctacatcc catgcacctt cacattgatt
    25741 ggccgctgtc gctccgcccc ataaaaatcc ttctggaaat tttttcatat cgatccccct
    25801 aaacttgcta gttttcttgt ttgattattt ttcttatgac tagtaatgat atttttgaat
    25861 ctactactat ttgtaacaat aaccattgtg gtcggatcat atccctcttt gattaattct
    25921 tgcaaatcta cttccatcaa taaatcgcct ttacgtactt tttttccatc agatactgca
    25981 ctttgaaagt atttgccatt taaattcact gtatcaatac cgatatggat cagtatctct
    26041 gctccttctt cactgactac cccaattgca tgtttcgttg gaaaaactgt tgaaacttcc
    26101 ccgtttacag gactgtagat ttttccgtcc gttggaatga tcgcaaaccc ttctcctaaa
    26161 gatccactag caaatgttgg atcgtttact tcagataaat caattatttc acctgctgca
    26221 ggagtactta acactatatc ctttgaggat tcttcttcga tttcatcctt gtagccgaaa
    26281 agaaaagtta gcatacagga actgatcatc gctaagaaat aactagcaac tagccaaaca
    26341 aatccttcac caatataaat cgggaaagac aaaatacttc ttgtagcaac tgccaaagca
    26401 ccggctcctg cggcaccagc tactccacca aatatagctc cacatataca agcaatgtaa
    26461 aatggtcgtt tatatttaag agtcacacca taaacagcag gttcagtgat tccaaataat
    26521 gctgtgataa atgcagataa agataaactt tttactttct tatttttcgt tttcaaaaat
    26581 accccaagta ctgctcctgc ttgcgcagct acggcagtca tacatgctgg acctagcgta
    26641 tcacgaccgt agaaagaaag attgttggtc attactggta caagtcccca atgtaaccca
    26701 aaaatcacta acacttgcca aaatccgccg ataacagcac cagataaaac agggctaaag
    26761 ttataaacag ctaaatatcc agctgcaatc agctgacttg cataatctgc aattggacct
    26821 acaactaata aagtaattgg gaccatcacg atgatacaaa tgaatggtgt caataaattt
    26881 cgaatacttt cgtgcaagct tttgcgaatc ttaggctcaa tataagaaag aacccataca
    26941 gctaaaattg ctggaataac cgtacttgta taccttgcta gaatcacggg catccctaga
    27001 aatcgcaagg ttatgctatc actatatgca ctgacaatcg taggataaac gagagctgct
    27061 gcaataacca tagcgatgaa acgatctgta ttgaactttt ttgcagcagt atatgccaaa
    27121 aatataggca agaaatagaa aaatgcatca gctgctgcat tcaaaatgat ataagttcct
    27181 gattcagtag ttaataaatt caaactcgta cataaggcga gtactccttt gatcaaacca
    27241 cttcctgcca agacgcctaa agtcggggca aaaacagcgg cgatcatgtc tataaaagag
    27301 tttaaaatgc cttttttctc agttcctctc tttccactag atgcatcatc ttctagtttc
    27361 atttgtttgg ataatacgtc atacacttca tttactgcat ttcctactac gacttgaaac
    27421 attcctccag cttcgactac agtgatcaca ccttctagtt gctctacctt ctccttatct
    27481 gccttcttat tatcttttag ggtaaagcgt aaccttgttg cacaatggta aacattgctg
    27541 acattttctt cgcctccgac aaatcggaca atatctttcg ctaattgtga atagtccatt
    27601 tttacttccc ccttgttaat taatgccgta gcaatccttc ttacaagata agtatagttc
    27661 ttagaaaacg gtttctccat tcaattagta ccccttataa tagcgaacga aactgtacaa
    27721 tagtgtgtat ggtttactta gccgagcaaa tcactcactg cttgaaagaa aacaagtata
    27781 tcgtttctct tatagtcaaa ggagacaaaa aaaacacgct tgatcttcct ttgctgatgt
    27841 ataagtggta tctcaagctg atactctgtg atttttgttt ctacttggct tttgataaga
    27901 cagcacaaca aagtctcggc atttaacgga agcataacta ggtcagaact aggagagatt
    27961 agttgctgaa tggaatctat ctttttatgg atttttattt gattttcttc tttgtagtaa
    28021 tgatcaagca gttttgtttt ctttttttct tcaactatgg tcagcttttc acgcaaaatc
    28081 tgataaatct cccaagattc aaactcatct agaacactag gtagataaat caaatcatcc
    28141 cactcaaaaa taagctcttg ctcagtagtc aaaattgcat catattccgt tattttgttt
    28201 gattgatttt tgaaaagata cactggttct atccggcatt ctttgatcca catgttcata
    28261 ctttgttgaa tctgtttttt tagtgtattc aaaatactaa ttggctggtt actaacaatc
    28321 agtatctccc cttgttttcg aaaaggattc aacgcttcgg caagatacaa aacaaaataa
    28381 gcgctttcag ctaaattggg acgtaattga aaatgtattg ggaagaaacg ccgaattaaa
    28441 taaatttcta aaggataacg ggagatgatc ttttgtacaa aatgattcat ggtattatgt
    28501 ccggcatcca tcctcgtttt catttgtttg atatgggtta gcaaaaattc tgtttcatgg
    28561 aacaataacg aaacaggaag atctaaaaaa cgaataatcg tttgctcaaa tgctcttaga
    28621 ttcttctcta tttctttgtt tttttcatca ttaatcatca aataatttaa ctctaatagt
    28681 cgattttcaa ttgcttgttt ttcttgattg gaaaaagaat agggtagttc tgatgataac
    28741 ttttttgtga gggtctctac catcggaata aataatgtat acctgtttgt tttttctaaa
    28801 agttcttctc ttcgaagaat ggtcaaaacg agataccgag aaaatcgtat gaagtcctct
    28861 cctgaaacaa tataattata tgatataaga acctctttta atgtcttttg gacagtcggc
    28921 atgatattgg aaaaaattgt actgatttgt tgaggcaacc ttgcgtgttg gatcaccgtt
    28981 tctgttccaa caagagataa gaaaattctt tttgaatttt cttcagcaat gatttttagt
    29041 ccttttgtcg acgaaacttc cagttctaat ttaggattgc gctcgatcca acgcaaaatc
    29101 gtttttattt gtaatgatac ggctgttttt gaaagaaaga aaatgtctgc tatcttttgc
    29161 atactgacat accctttttg aaacaataag tacgtcacaa tagttgaagc tcggaaatcc
    29221 atgctggaat agattttatg tttcgtcacc tcttgtatga tttgctgttt agtttcagat
    29281 tcaattgatt ctaaaaaata acctgaacgt ttatttgact ggataatgat tttcttctcg
    29341 tagatagcaa gcatattttt gatttcagtt tggatcgttt tcttgttggt acctaaagct
    29401 tcagctaatt cttcgcttgt tatccaatga ttcttatttc ttaatactac aagaatttgg
    29461 agttgtcgtt tatttaagtc catttgctta cacctcctct ttgttataaa catacaggaa
    29521 aaacctctca aatgacaata gtcacttgca gaggtttaaa aatcatttga agaaagatct
    29581 atattcaagg tttttaccat tagactggat aatttgttta taatattcaa aactatcttt
    29641 tttcaatctc ctaaagcttc cgctcccatc attttgtcta tccacataaa taaagccata
    29701 tcgcttcctc atttcccctg ttcctgccga tacgatatcg attggtcccc accataagta
    29761 accaatgatt tggcaaccat cttttattgc ctcagcaatt tcctgaagat gtatttttag
    29821 atactcacaa cgttttggat cgtggattcg attcaattca tctggtgctt catctagtcc
    29881 aatgccattt tcaactatga ataatggtaa ttcataacgg tctgtcaatt cattcatgac
    29941 ataacgaaac cctagaggat caattggcca actccaaggt tcgggagatt tttccttcaa
    30001 aaagggatta tcgaaaccat ttgctccgcc agtatctacg cgcatttctg cttctttttt
    30061 atagacagcg ctacgataat aactaaatgc aatataatct gccgtgttag cttgcaagca
    30121 ctgttcgtct ccatctaaca ttactggtgc acagtcttct tccttccatg tcctgtaaat
    30181 ataagctgga taatgccctc ggcacatgac atcggagaaa aaccatgaat tgcgtttaaa
    30241 ttgcagtgaa ccaaatacat tctctggatc acagtcatat gcataagtcg ctaatgcaga
    30301 aaagctacac ataataccca tcttagcatt tggatcgatt ttctttaata attgagtagc
    30361 acgggcgttc gctagaaaca tatgatgaca ggcttgataa atcacttttt ttgttagtgt
    30421 agcaatgggt tcaagtttat ctctagcagg gaatgtaacc aatccagcag ctgcataagg
    30481 catacgaaaa gcattattga tctcattaaa ggtcatccaa tatttcactt tcccttggta
    30541 gcgttcaaag acggtctgcg cataaaattc aaaaaagtcg attaattttc gattttgcca
    30601 ccccccatac tctcctacca atgctaaggg ggtttcataa tgactaagag taattactgg
    30661 ctcgatatca tatcgattta atgtattgat tagtctgtca taaaatacga gaccagcctc
    30721 atttggcttc tgttcgtctc ctcttggaaa tattcgtgcc catgagatgc ttgttcgaaa
    30781 agcttttaaa cctaactcct tcagtaaacg aatatcctct tcaaatcgat gataaaaatc
    30841 aatcgcttca tgagaaagat aatggagtga ttcgtctaaa gcaatctccc aaatcttttt
    30901 ttcttcattc cactgaatac taggatgctg gttcatgatc ccgataagcg tatctgtcac
    30961 attaggtaat ttgccgtctt ccaaccatgc accttcacat tgattagcag ctactgctcc
    31021 gccccataaa aaattttctg gaaatatttc ctgatccata actatctctc ctcgctatca
    31081 acaatttctt acttgaagta tagctcgatt catcaaaaca agatattcta actatttcgg
    31141 cagaagagaa gaaaaaaatt gaatgctact cttcattttt aatatttata tataacaaat
    31201 acataagacg atccccgaaa gaaaacactc gttaaccatc taagcaaacg atttatttta
    31261 cttccaacac aaatgcttca taaggtcgta aatcaacttt gccgtctggt aactgtttca
    31321 gaggataatt tgacacaata acccttaaag cttcacgtgg aacagtgaac gtctgctttt
    31381 cactggacat attacaaata acgagccatt gctggtcttc atatatgcga gaataggcaa
    31441 agacatgctc aggcgtcttt tccactaatt catagtctcc ccagacgatg atgtcatgtt
    31501 ttctacgcaa atcgatcaat ttcttgtaca tataaaaaac agaatcttga tctgctaacg
    31561 cttcttctgc attgatttct ttataatttg gattgactcg aagccatgga attccagtag
    31621 taaacccagc atgttcacgg gcgttccatt gcataggcgt cctagcattg tctcttcctt
    31681 tcgcattgat tgattccaag atttcctctt ttgtaaatcc agaagaaatc ctttcgttat
    31741 acatattgat tgtctcaata tcttttgctt ccgcgatatc atcgattggg caattcgtca
    31801 tgcctaactc ttctccttga tagatataag gggtgccttt catcatatgg agcaagatag
    31861 caaacatctt ggcactttct tttcgataca catcgtcatt tccccaacga gaaacaattc
    31921 gcggaagatc gtgattattc caaaataaac tattccatcc ttcatcacct aaagatgttt
    31981 gccatttgga taatactttt ttcagttcag agatactcaa gggtttcaaa tcccatttgt
    32041 ctttgccttc ttgctgatct aatcctatgt gttcgaattg gaagaccatg gataattcat
    32101 ttcttttagg attggaataa agtttagcga tttcggtagt tgctccccat gtttcgccaa
    32161 cagtcatcag atttttgtca ccaaaagttg cttgattcat ttcctgaaga tactcatgca
    32221 gtttaggtcc attacttgtg atttctttat ccggttcttt gccaatcata tcgatcacgt
    32281 ccatacgaaa gcccccaata cctttatcga tccagaaatt catcatttga taaatctctt
    32341 ggcgaacctg tttatttttc caattcaaat caggttgacg cttgctatac aagtgcagaa
    32401 aatattgccc cgttttttca tccaactgcc aagcagaacc actgaatatt gagcgcaatc
    32461 tattaggctc ttttccattg accggatcac gccaaatata atagtcccga taaggatttt
    32521 ctttattttt acgggcctca atgaaccacg gatgttcatc agaagtatga ttcaccacca
    32581 gatccatgat gatacggata tttcttttct ttgcttcttc aatcagttct tccatttctt
    32641 ccatcgtacc gaattctgcc atgattgctt gataattact gatatcatac ccattgtcat
    32701 cattcggaga ctcgtatact ggagaaagcc aaattgcatc aatacctaaa tacgccagat
    32761 aatccaacct ggaaataatc ccccgcaaat ctccaatacc atcttgattt gaatcttgaa
    32821 aacttcttgg atagatttga taaacaactg aatggtgcca ccatttttgt tccatgattt
    32881 tcctcctata aatggatgtt tctatttttt gcttaatcat ttattttagt ctctctcact
    32941 aaaaattgta acgctttcaa atagctattg caccttgtta tcggtaacag aataaaacaa
    33001 aaaaacagct gaggaaattc cctcaactgt tttttgaaag gattgattta tgcagggaag
    33061 aatgctgatt tcacaacttc tttataaacg gtgttcactc agctgtccct tggtattagc
    33121 tcaaaaaacg gcaaaatatg aggaactatt ttttcgtttt tttctctaat actcaggcca
    33181 gaacactgat ttcacaacct ctttataaac ggtgttcact cagctgtccc tcggtattag
    33241 ctcaaaaaac ggcaaaatat aagaaactat tttttcgttt ttttctctaa tactcaggcc
    33301 agaacgctga tttcacaacc tctttatttc acattcaata tccacatgaa tcccacaaat
    33361 acaaagaaca gaccatacat gattggagat acttcttttc ctcttttagc tgctaacatg
    33421 gtaattggat agaagataaa acctaatgca atcccatctg aaatactata agtcaaaggc
    33481 atgccgatca agatcaagaa tgaagggatc gcaatctcta agtttcccca ttcgatttgt
    33541 cgcaaagatt gtgccatcaa tactcctacc ataatcaatg ctggtgcagt tacttgtgaa
    33601 gtaacaactg atagtaatgg agaaaagaac aagcctaaca agaagaaaat ccctgtagta
    33661 atagctgtga tacctgaacg tcctccaacg gcgataccag ctgatgattc tacaaatgca
    33721 ccaacaggag aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc agaagctaaa
    33781 gctttcccta cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca
    33841 actaaagttc ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac tacgacccac
    33901 atttgaagag aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa
    33961 cttggtgctg cggcaacaat ttcagaaggc attggaatca aacctgtgac cagacctaaa
    34021 atcgttgtag ctgctatccc gatgaagatg ccacctggta cacgccgaac aagcaagata
    34081 gcagttacga ctaatccaaa aatcgtcaac caagtagaac caacgttgag tgatcctaat
    34141 cctactaatg tagattcatt cgaaacgatg atcccacctt cgctcagacc aaggaaagcg
    34201 ataaatagac cgataccgcc agaaatcgca tatttcaaat ctgctgggat cgcatcaatg
    34261 atcatttccc gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg
    34321 ccggctaatg ctgtttccca tggaatcccc attcctaaac aaacagaata agcaaaaaaa
    34381 gcattgatcc caagagcagg tgccgttcca attggataac gggctaatac ccccattaaa
    34441 acacagccga tcgcactggc taatgcagtc gcagtaaaca ctgctccttc atccattcca
    34501 gaagcaccta atacagttgg atttacaaat aagatataag ccattgagat aaatgttgta
    34561 aatcctgcca gtacctcacg ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa
    34621 agtatttttt ccttcatctt ccatccccct aaatccgaac attataaata aagtccgtta
    34681 aaatttcgat ttattaataa aacaacgtgt tttatcatat actaaaacaa aacataagta
    34741 aacaaaaaat tcggatttta agcaaaatcc gaacgttcca tctgattaaa tccctttatc
    34801 tcaaagttca tgaacattca ctttggatta caagaatttc tttattgtgt taataaagtt
    34861 tcttcattct agtctcaaaa atcaatagtg tgatttgatt ttaccatttc gtttctgttc
    34921 ttctttgatg aattcttttg tatcgacagg attcagatta tttagtccgc caaaacctgt
    34981 atcgaccatt tttccccagc cttttgtctt cttttttcct gatttgagat tctttgctcg
    35041 aagcttctca ctatgttttg ctcgccgata cttcaaataa ccttctaatc ttcccattag
    35101 atccactgtt tttcctcctt tttattcaat cagacgactc agcttttcct ataacaaaat
    35161 aattgccttc agggtcagaa aaattgaaag tcaagatgcc attgttatct gtgatttctc
    35221 ctggttcttc catgttctcg tagatagttt caaattcatc cgtaaaaaat actaaagaag
    35281 gcggtggtcc taatacctct ggtgaaaact tctgcacaaa tacttttggg aatacgccta
    35341 attctaattc ttttgagatg ctgagtacaa tactcttaaa ttcttctggc aattcgattg
    35401 tttcagaaat ttctgcacca attttttcta caaagaatcg gcaaatcatc tctacgtcat
    35461 tgacataaag catcacacgt gttttttgta tcatcatttg acccttcttt ctatttgatt
    35521 ttctttcctg tctataaact gattccatta tatcacgaaa gcgcaaacac cttgtgtttg
    35581 atactattca agaaaaataa agcaatagtt tctaggattg ctttcctcca caaacttttt
    35641 ttacaaaaaa aaaaaacggc aatgaatgcc gctcttctta tttcgattct tttataattc
    35701 tgcttctgat tcatctttgg ttagtgtgtg tctaacacta gttacgccaa atgtttgatc
    35761 gattttttcc ttcaacgctt cgattgcctg tgcgccacgt gccgcttgtt cagcattgat
    35821 ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc agcattgttc tttgggcttc
    35881 acgccagttg agacagcgac agatcgctcc ttgttcatca tagtagatga tttcttccgg
    35941 aagtgccgga gcatcggaat ctgcacctaa aggcaggaat gattcgccac cttgggcttt
    36001 tcctaaatgc agatttcctg cgatcttttc gatatcttct ccgccgcaag gtacgccaaa
    36061 agacaaggaa atactattat agatatctac aagcggattg attgggaaaa attcccgttc
    36121 ttgatttgca cgtttcagta aagcttcaat agaagaacgt gcaccttttt tagtcttgaa
    36181 tgtggtaaat gcctgacgcc actgagcaat gacttcgttt tggctgaatg tttcttctgt
    36241 aaggtaattt cttgcttctt ttgctgcctc attcaataat tgatgatacg tttcttcact
    36301 ttcttttgtt acgtggttat cgataccatt tacggataag atttcgatgg ttgcttcagg
    36361 aaatacttcc cagaaattgg tatccactat tacttttttc atctgttcac acctcttgat
    36421 tagaataatt gcttgatgaa tagatagtct gtttggggat catctcccat ataaaaaaca
    36481 tgttcgctgt atttgataaa tcctttttta cgataaaaag cgagcgcaac atcgttgtgt
    36541 tcccaaacac cgagccaaat ggctgttttt tgcttttctt ttgctttttc aatagctgta
    36601 tctagtaaaa atgtaccgat accttgatgt ttaaattttt ttcgtacata gattcgctcg
    36661 atttctaaaa aatgatcagc catttcctca gattgactcg ttcctgtatt tagtttcagg
    36721 taacccgcag gctcttgatc gatactcact aggaaaaaat cggtttcttt atcttttagc
    36781 tcttctgtaa gtttttctag tgcataagct tgtgtcaagt aatcagctaa atgctccggt
    36841 gaattatctt ttccaaatgt atctgtaaat gtttcgatac taatttgttg tagtaagtct
    36901 acatctgaag gcgtcatttt tcgaatttcg tatttattca cggcttcttc ctttcaatag
    36961 atccttttat tgcctttttt gacaaaattc caatcttcgc taacattctt gcatacttta
    37021 tttaaaagat attccaattg ttttgcttct tcgtcagaaa gaccatttaa tgcgactgca
    37081 ttggagtagt tgttttctcg aatgatgaaa ggatagattt ctgctccttt tttagtcgga
    37141 tagagtttct tgattttttt attttcttta tcttccaaac gttcgatcat cccattggat
    37201 tctaatttct tgattgctcg ggcagtcgtt gtacgatcta ctttgatcat ttctgctagt
    37261 ttttcttgga tgattccagg attttcgcaa atacgtacta aataaagata ctgtcctctt
    37321 gttaaatcga cttctttgaa ttcgatattg ctaatagaat ctagcgctcg ggcaatcatt
    37381 ccgatgtctc gaaggatttc agccatttca tcccaccttt attttgtttg taagactagt
    37441 ttacctcttt ttgttgcaaa tgcaactatt ttcaataaaa aaatcaagtt cttttttaat
    37501 aactagtaaa gtatattctt ttcttctttt acgaaaaagc tcaatcacaa tagatctggt
    37561 ttttgttttc taaaataagt aactgtttct tcgttgttcg cttttctgat agctttggca
    37621 actcttctat actaaaaaat cccatgttgg aagtttcatt attctcaata aacttggcct
    37681 cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata ctgaaaaagc tgtggaatat
    37741 ctttttgttt attcgtatca aaaactgctc gcaactgagt agtttctact gttaatcctg
    37801 tttcttcata gacttcttta cgcacatttt cttcaggaga caatcctatt tcagcaaatc
    37861 caccaggaag tgaccattct ttcgtccgtt tatcttcgat tagtaggacc ttgccatctt
    37921 ttttgatata ggcacgaaca tctacttttg gtgttggatg tccttctcca atcgttgtga
    37981 gcgcttttat ttcttcgaaa gtttcttttc ctatttcact gatcagttcc aatgaaatcg
    38041 tccggatctc ctcaaaacgc tctttatcaa atacatcttt tccataatat agaccagctt
    38101 gagaaagtgc gagtagtctt ttgtattgag ccaacttctt gttcattctc ttcccttctt
    38161 ttttagcaaa tgtatgacta tgtaatcaag tatagaagac tggtttaatt acggcaaata
    38221 aatataatga aatctgcaat aaataaaaga atgttaaaaa actcgagaac aaaagacatt
    38281 cgacttctct ggggctgtga ataaatggcg ttcaaaagct tgcatctgcg gtaataagcc
    38341 ccaccaaacg aaaaatatga cgagctattc ttgtctggct gttcttatta attgaggcag
    38401 gacagctttt tcacaacctc cccactaaac ggtgttctcc taccaactcc tcggtcacaa
    38461 gtcacaatat tcatcaaaca tgagaagctg tttttttgaa attgctcctt gtgactcgga
    38521 gctgaacgac aggctcacga cctctcggta aacagcgttc aaaagccaat ttttcgatat
    38581 taagccattt ctttgttact tattgtcatt gatttctttt tctaatttat ccaatagtgg
    38641 agtgtaatcc acttgagtat agtctgttac atcaaattca tatagtgttc ctaactggaa
    38701 tttgtcatat tttcggtcgg cgatgatctt tttgaatgct tctttcgtaa tatgattggt
    38761 ccctttcgtt cgatcttcca atacgtcacc atagtgataa tgatccatga tatagctcaa
    38821 atgacgatct gattcacgat cacgtttgta ccgacgttga cataacactt caaagtctgc
    38881 tgttaggcga atcgtgatca cctgatatcc gtatctatct gctaattctt ggagtcgtgc
    38941 tttttgctta tcactaaaag gatactcgga cacaatcact ttctttcctg cttccatata
    39001 caaatcaagt gctttgtaat agaaaggata aactttcgtc ctttcttgtt ccgcccgttc
    39061 ttccaaattg ttaaacccga tggattctgc atagtattcc ttgatttcat cgagcgtgat
    39121 ggcatacata tctggaaatc gttcccgtag tttattcata agatatgttt ttcctgtacc
    39181 aggactacca gccagaagaa tgaaatactt tttcatctca tttatttcct aagttaattg
    39241 gactttccaa gttcataaac agtccaatct tatgcttgac aatttctttt actgcttcat
    39301 ttgctggaat cacattatgg cgaagagact tgttgatttc tgtttcacta aaggtttctt
    39361 tagctacttt cgtccagttg actaaaagct ccgttccaac attgaatttg cggatcccgt
    39421 tattgatcaa ttcaggataa tcttcttctt tcacacctgt tcctccatgg ataactaggg
    39481 ggacatcaac tactgcatcg atttctttca gcaagggaat gtttacttct gtttttgatt
    39541 tgaattgtcc atgattcgta ccgatagcaa tagctaatgc atccacgcct gtagcttcta
    39601 caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc attttcagcg acatggattc
    39661 cttcttctgt accgccgatt gttccaagtt ctccttctac ggaaacaccg tgcgcatggg
    39721 catattctac tactgctttt gtcttcagga tattttcttt gaaaggcaga tgggacccat
    39781 catacatgac agaagtatat ccgctgtcga ttgcttcttt gatatcatca aagtctcggg
    39841 cgtggtctaa gtgcaagact gcatcaacca tttcttcttc tgcaatagct gtcattacat
    39901 tgactaacac atgcatgccg atatatttag cggtatcgac acttgtttga ataatgatcg
    39961 gcgaacccat ttcttttgct gcgcctagca tagcaggaag catttctagg ttatgcgtat
    40021 taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt tgttacttct ttcaatgttg
    40081 tatacatctt ttattgccac cttatctttt tatttgtttc ctttgatcgt tacttgtttt
    40141 gcaatttgtc gcatctcatc cattttgccc atgtagtaat cgatcccttg atcatcacca
    40201 gcacgagcag catctgcacg cttctggtta tagaggctca tcagtttttt atacccatct
    40261 ccgatcgtct gtttattttc caaactcttt tctaaatatt ggatggcttg atcgttcaca
    40321 tacaattcag aaaatttaag tccaacttgt tcataaagtt tcgctaattt atgcttatct
    40381 tgggtagtgg aaatctcttg tttcaattgt gtaatctctg attgaatctt ttcggtcgct
    40441 tctggcgata gggctttttc ttcttttgcc agaacttttt tcggttcttt ttcttttttc
    40501 ttaaaaaatc caaacatcat tttccctact ttcaattaaa atgctttcaa aactgggctt
    40561 aagatccagg catataagag tgctgcggct actgtatcta catcggttgc tgtagcattg
    40621 atgaatccca tcgaattaaa gatcgttact agcaaagcgg gcaataatgt aataaagaaa
    40681 ccatgagcaa taccgccaat gatcgctcct cggcgtccgc caactgcgtt gccaaatatc
    40741 cctgctgtcc ctccggcaaa gaaattggtt agcataccag gtaaaatcat cgctaagccg
    40801 aacatgggca aagtaaacat ggcaataatc gtaccaatcg ttgtcgtaat gaaacctaaa
    40861 atgacagcat ttgggctata aggaaagaaa actggacaat ctaaggcagg gatcgcatca
    40921 ggaactaatt tcatagctat ccctcgaaat gccggcacga tttcacctaa cagtagacgt
    40981 acaccagcta gcaatacata cacacccact acgaattgga tggcttgtaa aaaggcaaac
    41041 atgatatagt tttgatctcc tgataattca gaagcgaatg gttcgcctgc aaataaaaca
    41101 gtgatgatat atagcgggac catcacgacc atgacagaca aataagtatc ttgcagaaat
    41161 tcgaaatgtg tcggcaactt gatatcttcg atcgactttt tattttcgcc tttctcacca
    41221 aaaagcttag caacaccggc ttcaaataag tacccgatcg tacaaaaatg acctaaagca
    41281 atatcatttg atccagtgat tttacgaatg atcggttgcg ctactgccgg catagcaatg
    41341 gcgaaacaag ctccgataaa accaccgacc aagatcaata cgatccctcg cagaccggca
    41401 aaatagccaa agactgtcgt catcgttgcc atccataaaa ttgcttgacc agttaaaaaa
    41461 atgtacttcc attttgtgaa acgagccaaa ataatattga atacaaaaat ggctaagaac
    41521 gtcagtgcaa tatctctacc taaacccaaa tcattcattg cttgaccatt gatagattca
    41581 atagaaggga tgatcccttg catatgaaat ccttcagtaa agattttccc gaaataagtt
    41641 aaacttccaa cgatgatgct cgaaccggca ctcaacactt gaaatcctag gagtgtcttt
    41701 aacgtgccag agatgacttg tcctgctgat ttcttctgca gtagtagacc caacatagca
    41761 atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt cgatgataaa attgataacg
    41821 cccatgtttt taccctcttt tctttttgta tttagataac tcccttatct tgcagaactg
    41881 gggtgatttt ttcttcaatt tcattttttg aaacaatatt tttcaaatag atcatcgccg
    41941 ttttaggatc aactgcgaac ttttcgaact gcgaacgaaa attttctgct gtgatgatca
    42001 catctggttt catggaaact gcggaagaaa tatcacaatg gtccagtttg gcttctactc
    42061 ctaatttgtt caaaacatcc tcagcagaca tctgtgctgc aaaactactt cctaaacctg
    42121 caccgcatac gaataaaatg ttcaatttgt tcatctttgt tctcccccgt atcatttatt
    42181 atttctcaac tactgaaaaa agcagttgtt tgaaatcttc gatatttgtg cattcaatca
    42241 atcgatccaa cttctcttcg ttgttgatca attcaattag ttctttcatg atgttcagat
    42301 gcgagtagga gtctactgct gccaaacaaa aaataatctt tactggatcc atttcaggat
    42361 taccgaaatc gattggttgc tcaatcgtag ctacgctgac tcctaattta ttgaccccat
    42421 cttctggtcg ggcatgggct agtgccatgt gtttgccaat cacgatatac ggtccgtaat
    42481 cattcactgc acgaatcatc gcttcgacat agcgctcttc gacaatgttt tctttaacca
    42541 atggtctagc agctagttca atcgattcct cccaagtagt ggctttttct ttgatcatga
    42601 tattgctgtc cgtcaaaata tcttttaaca taggctggat ctcccttttg ttgatttcta
    42661 aattgtttgt ttcgaataat ttttctaact tgccatagat gattcgatct actcggccac
    42721 cactttcttt catgacatct attatttgag tgaataactc cgtcgattca tgactgttag
    42781 gctctaaccg ctgtagttct tcatttcttt tcaaaaagtc ttctataacc aagatattcg
    42841 tctcagttaa aatcggatcg acaactaaaa gcggcttatt tcgataattc aattgaaagg
    42901 tgctgaaaac cagattgaca tccaatttat ctactaattc gatttctttt gaacttaaaa
    42961 ctgcagtaat ctcgatctga ggaaactttt ctttcagatt ctctgctaac agatttccag
    43021 tagccatccc atggttacag acaacgactg atttataaac ataattcaag tctcgcttga
    43081 ttacactggc gatcgtcgaa aagtggatga caagaaaagc tatctcgtcc tccagtatct
    43141 tgccaccaat tacttcttcg atcgtaggaa caaaatgagt gactgcgcgg tagattggga
    43201 aataatttgt tcggatgttt tctttcaatg gattcatgac ttggatatga ttcttgattc
    43261 ttacaatcag tgcagccatg tgcttataga gattttcgaa taaagcttct tcctttagat
    43321 ggaaaggtac gtgtgtctcg ttctctacaa attggatcaa ttggatcgtt agtaattgag
    43381 cttgtaccca ctcgattgaa ttggagatat ctctagcatt gaacgtctca atagtgaaac
    43441 gaagataatt tctttctact tgcgggacat tgtccaattg aaattcctcg atcatgtggt
    43501 caataaatgc agaaaagcta ctgtctttta tttctgtatt tttccaatta atggcagaga
    43561 taaattcatg tttcctgatt cgctggatcc agatcaatgt aaaaagcagg atttgttttt
    43621 tataaatatc atcttcttgt ttcaacaact tattggaata gatctcctgt atttttttga
    43681 tttccaccaa aggaaaataa tggagaaaga ttttttgagc cggtgttaat tgtgcatctt
    43741 gattcataaa gaagtcggtc attcccagat tctgattgat aaaatcatag atcatcgtac
    43801 ggatagatcg ttctgaacct ttataaacca gtccttgctt tccataactg acgatctcta
    43861 tcccataact gaccagtgtt gctcgtaacc gtctcatatc ttcatctaat gtacttttag
    43921 agatttgaaa ttcttcttct tttttgttta gatggatcgg gattcggctg taagcaacgg
    43981 ctaaaactag atcaaaaata cgttcgcgcc gattcaaaaa gccgtctttc acatctttta
    44041 tttcagtttc taatcgagct tgcttttcct tttccagctt caacaaaaaa actttgttgc
    44101 gaattgtacg gatctccgga agctttttac tgctcaacca gatattcgct tcaagaactt
    44161 cctgacggat cgtccgctga ctgacattga actcacttgc tagatgtttt gcttttactg
    44221 gatattgggc tgtcatgatt ctgtgaagta tttcaattgt tcttgttttc atagaaatga
    44281 tctccttctg atttcaagta tatggttccg cttacataat aaaaagtaga tatttctgcc
    44341 acttgaagtg gaaagaaagt aagcaaagca aacaaaaaat aagccatatg ctttttcatc
    44401 tcctcaacga aaaagacata cgcctattat tacaaaaatt ttgcccatct atacaaaaaa
    44461 gaggttgtga aaaagctgtc ctgcatcaag taataagaac aactaaacta aaatagtgct
    44521 tcctattttt cgtttggttg ttcttattat cgcagatgca agcttttaaa caccatttag
    44581 tggagaggtt gtgacagaga tgttcagcct tgagcgataa gaagcgattt tgaaaaatcg
    44641 cttcccatat tttacaaaat cgtgccttat tgccgatagg ctatctctgg cacactgttt
    44701 attcagaaaa aggagctgcg acaaggcttt tgtcacaact ccgttcataa atatttcctg
    44761 tcaatccagc ataatcggct gaataaaatc ggcttgccat cttttttctt tctcagaaaa
    44821 atcaaaatac catatctgag aaaaatactg tcgttgtgtc ttacttcctg agatcaatcg
    44881 cccatctttt cgatcgactg aataatcaca gcatctgaaa tcaattcgga tggcaaaact
    44941 attttcgtgg atctgtcgat aatttcctac ttcctgaata gttatttttt tagtatgatt
    45001 tcttacaccg tctgctttgt ttttttgcag aactttctga tgttcggcaa acagcttgtc
    45061 tgtataacag cttctggctt tttcaggagt ttcttcttcc caagcggatt ggatcttcat
    45121 gaatatcttt tggatttcca gaaactgtgc tttcttttct ttagccgttc ctgacatgcg
    45181 ggacaatatc tccgctttca gccttttctt ttgtctttta ttcttgacga tagaaaaagc
    45241 agaaacccct cctactgaca aaacagctaa tgcaagaaat cccgtttgct cataactaga
    45301 aggacctgta cctggaccat aatacccata agaatctctg ccgtaagaac gactgccaaa
    45361 ataactgccc ccatatgaac gaccaccaaa tgaacctctg cccgaacttc gaccgctaga
    45421 tgtacctcca ccatgaccac ccgcgacagc gaatccttgg gtcggcagag caaacaaaca
    45481 taggcccact aacaaaatga ttgctaaata attgattttt ttcattgtct tcctcgttat
    45541 cttttattac aaaaaactat ttttatgata gccttgctaa aaggaagagt caataaccag
    45601 atgcataaac tgttttttta gcaattttgt cttatttagt cattgagtaa catcggacaa
    45661 atgtatgtgt cggcagtcca taattcctct atcttcagca tgaacaagct tgcagacaag
    45721 agatctgcgc ttcctccagg agagatttgg ttttcctgac aaaagtgcaa cagtgctgta
    45781 aatggacgat ttggtaaagc actttgacta gcagcatgag caagtttttg catttccact
    45841 aacgttttct gatcactgcg atgcaatacg catgtgtcat ctagcgaact catcaaaact
    45901 aacaacatat gcatttgttt ctgtacatat gaatcattat ctagctcctt agctgcttgg
    45961 attgcaagcc tgatatgcgg ataccctaat atcgcttctt catatgcacc atttacgcca
    46021 tatttttgct tcaccctatg accatgagtg gtttttttat gacgatattt tgtatcagga
    46081 aaagcagcaa gcttgctgac atcatcaagt attttcgata aagaaaaagg ctgttccata
    46141 ctgatttttt gtgcaatgac actgatcaat agccccattg cccaaatcgc tcctttatgg
    46201 gtattgatgc catttgtagc aatcagcatt gccttttccg cttttcgccc aatctcagca
    46261 attctttctc ttaactcctg atcaatagca tgatgccatg ctatttgagc catttcttca
    46321 aaaaaaggag ttagcgtctt agcagactta agaaataaag catagtccat atcggaatgt
    46381 gcacccgcat ccagtgcatc cactaatcca ggttttggac ttaatgttac ttctgcgatc
    46441 aatgcttcct ctaccaacgc agcgagttgc tgagtcactg ctttttttcg ttcgtcattt
    46501 atcatgacac tcactccttc ttcctactat tacttgctga aacctttttt gatagctacc
    46561 caaatgatat gaagtaacca gcttaaagca aatgcacaac ttgccgtcat tagataagct
    46621 acaagaatca tgactcctga aggtaactgc agccaactat ctttaaacaa taataacagt
    46681 acttggaaaa caaatacatt ggctaaatac gtacgataag cataaacaga caaccatttg
    46741 aacaatggca attttggcga atcgtttaca atcattttgg aagcgcctat gaagacagca
    46801 aagatgatga ctaaactgta taaactttgg atcgtattca agtaaggtgc attcgcaaag
    46861 ctcaagtctc ctggatgtgc gagcaatgaa cggacagaaa agaacgcgat aactaaggca
    46921 acaggtaaga aaagataacg tactctgtcc aaatattgat agactttttc gtgataaaca
    46981 aatgcggcta ccccataaat catgaaacta gcaacaaacc gatctaataa gtaccagctg
    47041 tcatgataag gcccagtcaa tacttgtgtt tgatagaaca gataccatgc gacatataaa
    47101 atcgttgaac caatcaatac tggccataca tattttttat tagcaaatac tttgtaaccg
    47161 agccaaacaa aaaacggcat gaacaattgg atctgcagca tcataacggt ataccataaa
    47221 tgaggcgctc catcacctgt gacatatttc agtaaaaagc ttgcagtatc cgtatacgga
    47281 gtttgttgtt ggactgctgg aaatagccaa agataagccg tcgtccatag aagatacgga
    47341 acgactagtt caaaaaactt ttctttcaaa aattcccaat aagacaaatg ctgacttgtt
    47401 tttaccatat tataaacaat tgcaaaaata aacattggtg ctgtgtattt aacgatgacg
    47461 taaaaggtac caagaaaagc tgtatttgtt gcgctgtggc tgtttgccaa agcaaagctc
    47521 aatatcgttt gcatcataac agccgttaca gctgatactt tctggaagtc acttacgttg
    47581 attttgattt ttttgttttc aaacatctct ttctctctct ttttctttat taccaaagtt
    47641 ctttggttaa tttaggtcct tcgactgttt tcaatagaat ttttttctct ggctctctca
    47701 aatactcttt tagtgaaaaa ccgccttgcc gagtcactac ttgggtatcg aattgctcaa
    47761 aaaaagcagg atgcgattga attacctgaa ttggcaattc aatcggtgaa tttgcgtaca
    47821 gcagcaaatc aaaatcactg gtttctttta ctgccggaat cccagtcgat aattcaaatc
    47881 ctaagctgcc gccgattccc catttacatc ctgataatat gtttctagca gcatgaaatt
    47941 gctgataaac aggataatcc atgattttct cttgtctata cgattcttgt tcgatgatgt
    48001 cccacggatg aatgacttgt ttgacggcat gttttgagat ttctgttgac catctctgat
    48061 tgcgggcagg tccacgtaaa ccaatcggta gtatttctgc gtgatccgtc gacgcacgac
    48121 gaactgttcc gaacatcttt tcagttagaa cgacccattc aggcaaggtg aacccttcta
    48181 tttcatgttt atcaaaaagg acaatatcat gtgcttgaat cattttttat gcatcccatt
    48241 cttcgtccat tttggcacgg actttatttg tttcgacacg tccgaatgtt ttggctgttt
    48301 cgtttgtgta tcgatttgtc agcatcgtgt catttgtatt gcggacatct tgaatggctt
    48361 cattgattgc ttcgatgact ttgctgacat tttgttctga agcagcttgg tcggtgatat
    48421 cttctaggaa atgatagatc gctcctagtt ttgtataatt attgatatcg taagcgatag
    48481 ctggaacttt ttcagcagct gcttcgattt cagcaaccga acgttttgtc acacgtgcag
    48541 cactttcttt agacatcgct tggacggtaa tttctttatt gtttaaagca atcaagcggc
    48601 ttgattgtaa tccatgtgct aaaaatgcac ctgaaatagc atttcctgca atcaatccaa
    48661 taactggatg tccagcttga cggagttttg cataagcagc agcactatta gccaaagcca
    48721 catggatacc gatcaattct tcgttatagc cgtatgcttg gcttggtaca tcgatcacaa
    48781 cgatgattgg ccgtttcact tttttgtctt tatcttcttc gtaaacgtga tccaaaattt
    48841 gtcccatgcg gaaaccttca actaaaccaa cttcaccgtt tcgtacacgg tacatcgggt
    48901 ttttaggatc aggtacgatt gatgtcacga tggcttcttc tatgaattta cttgatttac
    48961 cataatacac agtactaata gaagaagttg gatttttcat gccagttagt gccgcaaacc
    49021 aatcgtaacc aacacttgct gcagctggtt catttccttc tgctacttct ggtaagtcaa
    49081 ttgattttgc ttgatggtta gcaattgctt cattgtaatc gtcaatggcc attggttttg
    49141 aaagatctaa agaatcaagt aatgataaat aaaatgctga gctttcactg cgatgcgtgt
    49201 cttttcgttc gtccattgct gcgatgacag cagctttgat tgcttctaca ctatcagtca
    49261 ctaattcatc aatgattttt gattctaccc gttgacgaga tcctaatgta ttccaaatca
    49321 agtctttgtc actagaatca aattctcgaa cgcctgcttc ttgttcaata acttctggtc
    49381 cattcaaacc gactctcgct ttttttgttg cgattaaata actcattaat gctgaagtaa
    49441 tcgacatacc gccaaatgat cctacacgtc ctggaatcaa accgatgact ggtacatatt
    49501 gttttagagc aatgattaca tttccgattt ctgaaataga taacagtccg tagtttgctt
    49561 cttgcaaacg aacaccacct gtatctagta caatgactgg aaagatctca tttccttttt
    49621 tattttcttc taacacatgt tcaagtgcag caacgatttt tgcaccggaa acttcaccga
    49681 taccgccacc ttggaatttt ccttccatag cgattactac tgcttgtttg ccattgatcg
    49741 tgccacggct gacaacgatc ccgtcatcac tttcagggac gattccttgt gctggtaaac
    49801 ttggcgcgat gatttgatcg aatggatcaa tcaattcacg tccattgtca agtagagcgt
    49861 tcactcgttc tctggcgttt aattctacaa aactattttt catctgaaag ttcctccatt
    49921 gcttgcgtca atcgtaggta cacaactcct ggtgtagcac cgaagtcatt gatcacgata
    49981 tctgctgaaa ttggataacg atcaaaaaat ctcgttaata cattgttcca tacttcgccg
    50041 aagccatcgc ttcctgtaca aatttttaca gtagattctg ttttttctgt tggaaacatc
    50101 aagatttcca aatctcctga accgaccact cctacatgga ttttcttatt gatcggctgt
    50161 ttcgttggat atttataagc taattgttcc atggatctct cccctttttg taaaacagag
    50221 catacacgtt ctgcttattc gtctttcttc ttccatctgg gtaaatggcg acaaaaggtt
    50281 tttctcgcca attttatgac caagttctga atttcattgg tggttcatac aggccatctg
    50341 accattcgat caaatcatcg atggtttgtg cagctaataa tgaacgtttc gcttcactac
    50401 gtttgatgcc tagatcttct ggtaatttca cgatgccggc tgcacgcaag ctatctagtg
    50461 attctttcgt gctagtcatt ccaagaggtg taacaccgcc aattgctgca attgctgctt
    50521 gtcgttcttc ttttgttttt gctttgtaca aataagcaat tccttcttct gtcacgatat
    50581 gtgtagtatc ttcgctataa atcatcactg gtgcattggc taagcctgct tgtttttgaa
    50641 cagctagtgc atctaattct tcgacaaaga ctggtttttt atttgaacca tatgtttcaa
    50701 ccatttgaac aactaatttt tggcctttcc ctaatggatc atcattgtcg cgaagtgatt
    50761 gccatgctgg tgtagaatga cgacgaccgc caggattatg tcccatgtta ggcgcaccgc
    50821 caaaaccaga taaacgtcct tttgttacgg tagaagagtt tccttcgtaa tctaattgca
    50881 aggttgaacc gataaacata tcgatggcat attgtcctgc agcttgtgat aatgttcggt
    50941 tactgcgcat cgtgccgtct ttaccgacaa aaaagacatc tgggcgagca gcaatatatt
    51001 tttccatacc aacttcgcca ccaaaactat ggatgctttc aacccaacct gattcgatgg
    51061 ctggaatcat cgttggatgt gggttcaata cccagttctt cgcgattttc ccttttaacc
    51121 ctagtgattc accataagtt ggcagtaaca attcgatagc tgctgtattg aatccaatac
    51181 cgtgattcaa agattgtacg ccgtgttctg cgtagattcc tttgattgcc atcattgcca
    51241 ttagaatttg caattctgtg atgttttgag gatctcgtgt gaataaagct tctaattgat
    51301 aaggtttatc tgctttcacg acaacgtcta cccagtcacc agggatatct acacgtggta
    51361 aagaatcgac aacttcgttg acttgaacga tcacgatacc atctttgaaa gctgcagctt
    51421 cgatgattgt tggtgtttct tccgtattga aacctgtgta caagttgccg tctttatctg
    51481 ctttatctgc agctactaaa acaacatttg gaatcaaatc gatgaataag cgtccataaa
    51541 gttccaaata agtatggata tcacctaatt tcattgtgcc gtcttcaatc atttgggcga
    51601 cacgcaagct ttgtgcacca gcataagaga aatcgatctt gcttgcgatt cctttttcaa
    51661 aaatattcaa atgttctggt cgtgagatac tagacatgat caaatgcaga tcattcactt
    51721 tttctgcatc tactttttca agactttctg aaagaaaact tgcttgtttt tgattgtcgc
    51781 cttctaatac aactttgtcg ccgggagcaa tcagtttttc cattacttct acgatttttt
    51841 cagtttctac atacttgcca ttgagcaagt tgctgatttt ttccaatcgc tgcgcttttt
    51901 tttcgcgttt agaagcccag ttgcggtttg gtttttccat aattagtcat ctccattttt
    51961 gatatagaaa cgtggcgtca tcaataccat gttggtctag attgatcgta cggatccctt
    52021 tatctacacc aaattttgta tgaatcagct ttgtcaaagt attgccgggg ggaaattcaa
    52081 tcgtcagatc cattcctaat tcttttgcca agtccatcat ttggttccac tgcacaggga
    52141 aagctaggtt ttccaacaaa tctttacgaa tctcttctgc gtcctgagtg gctcttccat
    52201 ccgtattttt tatatacaaa catctagcat cagatagatg gacagactgc atatacggct
    52261 tgaatttctt gaccgtatca ttcatcaaga gacagtgaga tggcacaggg actttcagca
    52321 gcttcgctgt ttgcgcttgg tgccgtttgg cttctgccaa cgtaacggat aaaccttcga
    52381 tactcccaga taacgtatgt tgcatcggac aattttcatt tgacagataa actggatggt
    52441 caggatcaaa tgtttttgct acaatttcct cagcttcgct tctcgtcaca cctactacta
    52501 cgcccattgc ataaccgctt ggataagctt ctttcattaa gactgcgcgg tgatagacta
    52561 gttccgctgc attctcaaat ctcaaagatc gacaagcaac agctgcacaa aaagcaccaa
    52621 tcgaatgtcc tgctgcaata tcaggctgaa taccgttttt ttccatttca tctaaataaa
    52681 atactgcttt tgtcagtaat gctagctgga taaaaaccgt atcttgatag ttttcttctt
    52741 tatcaactaa ccgatacccc gtcacatttt ctaaacgttc catgtacatt tcagggatat
    52801 ccgctaacat ccctaacttc tgactgcctt gtccaggaaa taagaaacct gtactcatga
    52861 gcctcgcctc ctcagcactt actttttcca ctgctatctt agtcaatttc tgctataata
    52921 aaaaatgcca aaaaaagaaa aaaccataac aaaaaggtat ggatgggatg gataattatg
    52981 aaattgcttc atctgcagta ttttaaaaaa gtattcgaaa caaaaaatgt tacccaagca
    53041 gccaaagaat tatttatttc tcaacctgcc ttgagccgtg cgatcaaaca tttggaaaac
    53101 gaattaggtg tccctttgtt ttaccatagc ggcagaaaca ttgagacgac cgtttatgca
    53161 gaagaatttt atccatatgc agtcaagaca ttagacacat tagaagaagg aatcaaatta
    53221 ttgtcttcgg tcaatgagaa gatcgttact tccgtcatat tgtatttaga ggtagcctct
    53281 gtttctattc cgaacttggt ccgaattttt atggaaaagc atcctgatat ccaattaacg
    53341 atcatgcaac atcatttacc agatgatatt accgatcctg tactgtacat cacttcggag
    53401 atgaagcctg gcatgaccaa tattcctatc atgaaagaac ctgtttatgt cgccattcct
    53461 aagaaacatc ctcttgctaa aaaagaacat ctgcaattag aagatatcca acgcaccccc
    53521 atcttgatgc tgtcaaagag caacgccttc cgtcatacac tggatgcagc acttgaagcg
    53581 aaaagtatcg aattgacgat cagttcgacg acagatgatc ctgcgacatt acgttctatt
    53641 gtcaagcaag gattagctgt tagctttttc ccaaaagttt cttggtctta cgataaaaat
    53701 gatcctttcg tcttgcggga aatcaaagat ttgcccttga cacgaacaat ttatttatct
    53761 tctagcttca cagaagatca tcctttgatc aaaatgattg caaaaacgat gcgggaattt
    53821 tatttaggaa aataaaaaaa aaagagctgg gacaaaattc ttgtcgcagc tcttttttcg
    53881 aacaaacggt gttccagaag taatttctta gaaaataagc cgaaatttcc caaaaatttg
    53941 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct aaacatttct gtcacaacct
    54001 cttctctatt gtcctagaca ttcattgcgt tgatttaaaa cttccatcaa tcaaatctgc
    54061 tagttcatct agcttttcta ttttacgtag atcaatagag atccaatgtt ctttattcat
    54121 gtgataggct ggaaaatacc cctcttttcc tcgcaaaatt ccgccaagtt caggatcgac
    54181 cttcaaatca agaatattga tgatcctatg gtcacttgca tcatcataga ttttttcttc
    54241 tggaacagac atcaggaccc caaaccattt tttgcttttt tgatgtctga atacgcaata
    54301 atcaggaaat tttttaaaaa tatattctgg agacaaattg tatgtttcat tgatgtgatc
    54361 gattagttct tttttcgtta gcatctgctt ccatctcttt cttattgatt cgctttattc
    54421 aaagatacca cgtaagaata gaaaaatgaa tacaagtagt tgctaatcaa atacttcgat
    54481 atttttcttt tttatcaact cttaaatgtt caatccgcct gtgttggtct tgctaccatc
    54541 tactattaaa atgtttatta ttgtttgccc acctattttt tgattttagt ggtatcattt
    54601 ttcaagttca tgatgaaatt cttatcatca gtaaatgctc cagaaaaata aacaatgatt
    54661 gaaaaatcag ttgaaacttg accaccgttt attttctccg tattcttaga atagaaggga
    54721 gtctgattga tgtattcact aatgaaaaaa atcattaccg aaaaagatat tcgacgtcat
    54781 gtttctttag tagaacaatt gctgaatcat acaaagataa ccgtaaatga attagctgaa
    54841 atcataggga caaccgaacg aacgattttt tctgaccttc aatctattcg ctctcagctg
    54901 cccgaaggct gggacatcat cagcgatcaa gcaggaatca gtttgcaaaa ccagcaaaat
    54961 ttattaacaa atgacctttg ggagattttt ttcaaacaat cagtcagtgt cgaactgctg
    55021 aaaaatctgc tttttacaaa aaaagttgct gtccctgatt ttctagccga ccatgggctt
    55081 tcttatggga cattgaaaag acatgtgact aaaatcaatc agcgtttagc aagttacgac
    55141 ttgcagatcg atttgacgaa atatacagct tgtattcttg gaaaagagcg tgcgattcgt
    55201 actttttatc atcgattgct gattcctttc actcataaca actatttttt cgaggattat
    55261 tcgatccatg aatcccatta ttttcaattt ttaagaaacc tcagtcaaac tgaactagcg
    55321 gttgaaacag aagagatttt cggaacatgt tggttcttta tcaatacgat ccgtataaaa
    55381 gcgaattgcc gattagattc ttctatccat accaacagca cactctcctc cctgtatgac
    55441 tctgcattga aaaaattgta tttaacggaa ggtatttatc ttaaagacac tgagctttct
    55501 tttgcttctt tctgctttct ggaaagttgg aattacaaca ataactatgg acaagaaacc
    55561 gctcgctgcc tgcatcattc tccattttta gaggtcctcg aaacgtttgt agaggaacta
    55621 gcttcagagc tgtctcttga tcaactaaaa aaaacatctc tgactgataa cttggctctc
    55681 ctcatattaa aatatcatga atcgccgata ttgattgaag cattggaccg tcaataccat
    55741 cgttttttag aaacatatga tcagcaatat gcacacttgt atcaacagaa agagaattta
    55801 ctgaacgatc taaaaaaaga aatagaaatc aatgaaccaa attactttct ccaactcttg
    55861 tctctactga tccaacaaac gattttttct atcaaaccga atttgttcaa tgtctatttt
    55921 attttccaag gagaaccttc atggaaggcg ttcttgcagc aggaattaaa agattactta
    55981 ggaaaacgtg tctgcttctt acctatcgaa ctaaccgatc tttccactat ttcttttgaa
    56041 aaaacagata tcttgatttc caatttccct ctagattctc tagatatccc tattgtatac
    56101 atttcttcta ttccaacgaa aaatgagctc aatcgtttga ctgaactcac tttcaaaact
    56161 tttttatagt aacggctagc gactgtattc tctagctgtt ttttcttttt tgcgaaacag
    56221 tttgttcatg atcccttttt ctatttctac aactaagaat agaagaatca catttgctcc
    56281 aatcaataac tgttgtgtca tcgttaaact tactgtaccg atcagctgct ggccgactgg
    56341 tagaaaaata accatccctt gcaataaagc aaggatacct aatgaaagaa aaagcgcttt
    56401 gttttgcaaa agaccttggt tcaatgacgg cttggacaac tctctgcaat tgatcatgta
    56461 aacagcttga gctaagacga tattttgcag aagaatggtt tgctgtaagc tttgcccttc
    56521 aaactgcata gccaataaat aggcaggaac catgatcagt agggagacat agatgatccg
    56581 aaacaaatga taaccagata ggattccttg ctttggatct cttggtgggc gcttcatgat
    56641 ttctggatct ggcgactcga accctagtgc ataagataaa gtgattgtcg ttaccatatt
    56701 gacccataaa atctgtactg gggacaaagg tagcgggcga ttcagcataa gtgcccaaat
    56761 caagatcaat ccttgagcta atgcagttgg caagaagaaa ttgatggttt tttgcaaatt
    56821 atccaaaatc cgccggccct cttttacggc ttttgcaatc gtatggaaat tatcatctcc
    56881 taaaaccata tcagctgcct gtttgcttac ttcactacct ttgattccca tagcaattcc
    56941 tacatcggat cttttcaaag caggtgcatc atttacgccg tctccggtca ttccgacgat
    57001 ttctccattt ttctgcaaag ctttaacaat tcgcagttta tgttctggtg tcgttcgagc
    57061 aaagacatcg actattggca cttgctgaat caactcttca tcagacataa gatccaaatc
    57121 gatcccttct aaaactttgg cagtatgttt taagccaatt tgttccgcaa tcgcttttgc
    57181 tgtgtctttg tgatcccctg taatcatttt cactgagatc cctgcttgtt ggcattcctt
    57241 tacagctttg actgcacttt cttttggcgg atcgatcaat ccggcgatcc ctgcaaaagt
    57301 taattgggtt aaagtctcat gcgtgagttc ctgtttcgaa tctacttttt tataagcaaa
    57361 tcctaaaaca cgctgacctt ttctcgcaaa ttctgcagct gtctgactcc atgcttcttg
    57421 ttctgcagaa gaaagagttg atttagcaaa taatacttct ggtgcacctt tgacgtaaat
    57481 caccgctcct ttttcctccg catgcatcgt tgccatatat ttgtattcag aactgaacgg
    57541 aatctttgct tctatttttt tcaaaggtag attggctgtt tctgtgtgtc gaatcagcgc
    57601 aacttctgtc ggattgccac ttaaattttc tgttttctgt tcttcttttg tttttaattc
    57661 ctgacagttt ttcataatgg acaacacgca ggcttcttct tttgcagcta catccacaac
    57721 tgtcatttca ttttttgtca atgttcctgt cttatctgaa cagatcactg tcatagaacc
    57781 taatgtttct accgaaggaa gtcctttgat gatcgccttt tcttttgcca tttcatgtac
    57841 tccaacggat agaatcatgg tcaaaacagc tggcaatcct tcgggtacca ttgacaccac
    57901 caaagcaatc gtagccgaaa gaaggatatg ccaatccata ccatatcgaa atgtcgtaaa
    57961 aataactaag aataggctca aacaaagaat gccttgaaaa atttgtttgt ttaatcggtt
    58021 gattttttta atcagaggcg tcgtttgttg ttcgaccgat tgcaatgctt gattgatttt
    58081 accgatttct gtcgtgtttc ctgtttctac aacgacaccg actgcagaac cagattgtac
    58141 aagcgttcct gaaaaagcca tatttttctg gtcgccgatc gattcttctt ctgtcaaaac
    58201 ggcatgactt ttttcagatg cttctgattc tcccgtcaaa atcgattctt ccactaataa
    58261 atcatgtgct tcgattaatc ggatatctgc tggaacgaca tctccggcac gtaagacaat
    58321 gacatctcct tttaccagtt ctttcgcagg gattttttct tgcatcccgt ttgatagtac
    58381 tatcgcttct tgtcccatca gctgcttcaa accattcaat gattcttccg cttttctttc
    58441 ttgccagtaa ctaacgagtc cattgacgat cactacgaat aaaataatac ttccttcaat
    58501 atattcacta gaagcaaatt tcaaaaaaga ggcaatgatc aacacgatca tcagcagatc
    58561 agtaaaatgc tttgctattt ttttccatcg ttttgtctgc tgccttgctt ttatttcatt
    58621 atatccttga tcagtcaggc gtttctgtcg ttcttcttgc gacaagcctt ctgttgatgt
    58681 ttgatagttt ttcaatacat cttctaccgt caatctgtac cagttcatcc tatcccctcc
    58741 tgtttaattg atactgttat tatacgcagg gaagtttttt caggcgtatc gattatttca
    58801 ggggggtctg aaataattcg ttttgcaaaa tgtatctggg cctaactgat atttctattg
    58861 cttattcttt tatatacaaa atttaagaat acaaaccaga taaccaataa aaaaggaata
    58921 aagcgtgtcg tgctttcgaa taacaagctg aaagcaattg aagtaaaaaa caccaacaaa
    58981 gcaagatctt tatatctttg tgtagtgttc cgtttctttt ctttaataaa ttgaacatgt
    59041 gcacacaaga tcgaagacca cacaaataga aaacaactcg ttgtcatgcc tgccaaaagc
    59101 tgaaaaaaca atcgactatc agctacaaaa aaatgtagac aaagaccggc aaaaaataac
    59161 attcctgtaa ttaaaatacc gtgtaaaggt acgcctttct tagaaagcgc acaaaaccaa
    59221 tggggcaaac ttttttcttt tgctgtttga tataaaatcc tgctagtact gtagactgca
    59281 ctatttgctg aggacaaaga agcaaccaaa atcattagat tgacaattgc tgctccgcct
    59341 tggcaatgga tcacttgcag catagtcact aaaggacttt cttttaaatc taaacaatgc
    59401 catggaacaa caaataaaat cgacaaaatc gttcctatat agaaaaagag aatacgccaa
    59461 ggcagacagt tgattgcttt tttcaaattc ttttgcggat cacttgtctc gccaacggtt
    59521 aagccgatca tttcaattcc agcaaaactg aataaaacca taggaaatga gactaccatt
    59581 ccagcaaagc caaacggtag taattcatcg atctgtccaa aattttcttt gattccgata
    59641 gactgggtat gaaaatctga gacactcaat cctataccca ataagataaa aaggaaaatc
    59701 cccaagatct tgatgattga gaaaaaagat tccaattcac caaaccactt gactgcaaca
    59761 aggttgactg ccattaagat cattagtgcg ctacctccgc ttatcaaagc tggtacatgt
    59821 acaaaccaga aatggatata caatcctatt gctgttaatt ccgcaagtcc agctccaagc
    59881 cagcaaagcc aataagtcca attgacaaaa aatgcccact ttttgcctaa ataatagtca
    59941 acgaaatcac tgatagagtt aaatgtacta tttgctaaaa ataaatcacc catggcccgc
    60001 atcaaaccat aaatagccaa accgcatatc aaataaacga gaataacagc tggccctgct
    60061 gatgcaattg cctttgctga acctaaaaat aatcctgttc caattgcgcc acctaatgcg
    60121 attaactgga tatttcgatt agttagagtc cgtttcattg ccggtttttc catcaagttc
    60181 acctttttct ttttcaagca gcatggcgac ttcctgttcc caagaaggtt tctttatttt
    60241 ttcaatacag agtggtacta caaacaaaat gacactgatg gctgtgactg caattagatt
    60301 gatccatgtt ccagctttca tcgtcagtcc gacagaaaat ccgattgccg caaataaaac
    60361 aataacgaca gtccaaactc caagattgct ctttttctct ttttttccaa ttctaaatgg
    60421 tcgattcaac tttgcatgtg tataacgcaa tctcaaaact gcgatcccca tgatgacata
    60481 gacgatacag tagataacag tagttgaatt tacgagcatc aaaaatgctt gattgacgcc
    60541 aggaatcagc aaataaaaaa gtgcaaagat tgagataatg acagactgag tcaaaataac
    60601 ggatttggat acgtcaaatt gattcgtctt ccaaaaattg aattttggtg gatacgcacc
    60661 ttttctagca ctttctgtca ctgtctttgc tggtcctgag gcccaagcag ataattggac
    60721 tgctacacca atgaagacaa gtaaactaaa cagattcaca ataagatgtg gcaaaccaag
    60781 aatttggcaa taaatcgaaa tagattgcgc gatattattc aattccattt gtccttttgg
    60841 tacgacattt gctacaacta aagcattcaa aatattgacg aaaaatataa aaattaacgc
    60901 ggcaaatact ccgcgaatat acgttttaac tggattttcc aatcgagtga tatacacaga
    60961 ggacatttcg ataccagtaa aaataaacat gatcggcgca aaataaacaa atgaggatgc
    61021 agtcgtagta tctggtatta atttgtccca tgaaaaactg cctaatgttc cggttggtac
    61081 gatcccaact ttgatccacg cagcaaaccc taataagagc atcatcgtca aaggcacgta
    61141 caatcctaac caaacaccga cttttccgcc gatttttgcc atatcaaatt tcaaattcaa
    61201 aagagtgatg atccaataaa cgactaaaat gacaactaat gtaaatttgc tgttcaaacc
    61261 aagcggcaca ttatcgataa tattaccaaa caacggagcg agtgctgaag cgaccatcac
    61321 catcccagga aacatttgga cccataacag ccatgagaca acgaaccccc atttttttcc
    61381 tagagcattc gatacccaaa gttctggacc acctttttgg ggaaacattc cagcaaattc
    61441 accagagatc aatgagattg gaaaagcgta caataatact gcaaccagca tgtaaaaaat
    61501 catcgtccag ctcgttgctg ctacatctgg aatatttcgt acacttgcta caagggcgca
    61561 ggtcattccg ataaaggtca tggtcgtgat ttgctttgat gaaccagtat ctttcatctt
    61621 tattccttct ttcaatagcg acatatgttt ttgttttctt ttatttttgt tcaagaacag
    61681 aaatgacaag gtttcatcga ttcagtacaa aacccagtat caccgaaaca tccattgaac
    61741 gatcttagtg aactgggttt gatacatgaa acgaacgatc caacttatag aacgttttat
    61801 aaaaaagaat atcttggcga ggaatatatg aaaaacaggg ggaaatacaa ttgttttgat
    61861 gaagcaaata attgtctgtt ctccatatgt ctcttgttat tctttcacac aaaaatcagt
    61921 tctattggat gatttcgttt agtttctttt ccattgcttt ggcgattttt tttgtataaa
    61981 aattgaaaat tcgatcatca ttcaaataag gagtcatgat agccgcacgt agcagagtga
    62041 ttttctgttc ttttttccat tcttctttcg ttagacccat tctttcaaca aaacgaatcg
    62101 gagaatcacc gtaatcttct tgtgtaaatg tagtatgaga agtaataaac cgttcaccat
    62161 aaacatcgcc atccatataa gaagaccgat cgaacatttt ttcatttaat tcattgatag
    62221 cgttcaaatc tgtacagcct tgttctttga atacccaatt gaccatatta aaatcaggat
    62281 gattcaatgg atagacttcg attgtttttc ctttaacagt aaaagtcaga tgatcgagaa
    62341 attctctgaa tctttgagct gcctcgattg aagccccaat caattgacca tatccggtca
    62401 cattcaatgg caatacacga tgtgctgtcc agactgcggc ggcggttgct ccagcttttg
    62461 atccttccaa aatataagct cctaacatat ctggagcctt tactgatttt tcaaacacat
    62521 atggcgcgaa ataagaaatg atatttctca tatttttatg tttgattacg attcctccag
    62581 cagcataagg gacatagccc atcttatgag gatctatcgt gacagaatcc gcttctgaaa
    62641 tagctctgaa cccttcatat acctctttgt caatcgttac gcagtggtgg aaaacatgat
    62701 gttcttcaaa gaattcagct aaggaagcat acggcacaaa ttctcctgct tcgtttagaa
    62761 acagtgaacg cgcatagccg ccatatgcgg catcaacatg taaatagaaa tagatccctt
    62821 catcttttaa cctttctctt aattggacga ttttatctac actgtctact tgaccttctt
    62881 ctgtcgttcc tacgacagca accacaccta gaattgggat tttttgtcca gctaattcac
    62941 gaattgtttt ttccaaagca ttaatatcca ttcgataatc ctcttgaact gggatagcaa
    63001 ccatctgatc taagccgact ccgcaaatat ctaacgcctt catccaagaa tagtgttttg
    63061 tttgagggac cagccatttc cctaatcttt ggatattttt tccgctacgg gaagaagctg
    63121 ctttgacctc atccatttct tcgtccgtaa agctttcggt catttccaaa atttcttcaa
    63181 cagataaatt caaaagttcc cactcagaca tttttttcac tttttcaggc agcacttcct
    63241 tgacagctaa aggaatcgat ttgatgcaac gggcgtacca taatccttca agattagcaa
    63301 tagaaccatc tgctgcaatg tgtccccatc cgtctgtcat attgaataat gaagcaaaat
    63361 cctgtcctac ttctgcttcc atttgcgaag ttgccataga agattccaat gcaacattat
    63421 ttgggttcca cagcatcgca tagttgtaag ctaataaagc aggcatcaat gtttcagcat
    63481 tcatttgtcc ccagtaacgg cctgctgaat gccatgggat cgatcctgcg cgtaatcgct
    63541 gactgacttc atccaagacc tctatcatat ggtcgcgggt ggctagataa tctggagtga
    63601 gcttatctcc ttcgctaatt gcaggcatat ctgaaggaat ataattttct ctccatccaa
    63661 gatgttcatc taccatcttg ttcaacaaca ttttataaac tggtccattt tcagctttgt
    63721 cgcctataaa gacggccttg atatccatat ctttcatttt tcttccctcc atattttgtt
    63781 ggtcaatgtc ttgcagatac atgtgtttat ctgtaacacc tcttcactat agcgccaaaa
    63841 atgagaaatt tttttttata acatcaaatc cacgtagatt tttacaaaca aaaataaata
    63901 aagtcggatt tttctgataa ttcaacgcaa aaaagcgaac aatttttcaa taaatcagtc
    63961 ttctaaaata gataacctaa aaaaagcgaa caaacaaata aaatgactgt ccttcattca
    64021 ctaaacatat ttttattttt ttatattgtt aaaaattcac
//
LOCUS       DACDTH010000009        57454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDTH010000009 DACDTH010000000
VERSION     DACDTH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.009"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1626)
                     /gene="treC"
                     /locus_tag="IWK54_000640"
     CDS             complement(<1..1626)
                     /gene="treC"
                     /locus_tag="IWK54_000640"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9592787.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(2023..4734)
                     /locus_tag="IWK54_000641"
     CDS             complement(2023..4734)
                     /locus_tag="IWK54_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9592788.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(4744..5445)
                     /locus_tag="IWK54_000642"
     CDS             complement(4744..5445)
                     /locus_tag="IWK54_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9592789.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            5631..6230
                     /locus_tag="IWK54_000643"
     CDS             5631..6230
                     /locus_tag="IWK54_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9592790.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            6311..7324
                     /locus_tag="IWK54_000644"
     CDS             6311..7324
                     /locus_tag="IWK54_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9592791.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            7432..8349
                     /gene="rbsK"
                     /locus_tag="IWK54_000645"
     CDS             7432..8349
                     /gene="rbsK"
                     /locus_tag="IWK54_000645"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9592792.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            8377..8772
                     /gene="rbsD"
                     /locus_tag="IWK54_000646"
     CDS             8377..8772
                     /gene="rbsD"
                     /locus_tag="IWK54_000646"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9592793.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            8772..10145
                     /locus_tag="IWK54_000647"
     CDS             8772..10145
                     /locus_tag="IWK54_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9592794.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            10502..12646
                     /locus_tag="IWK54_000648"
     CDS             10502..12646
                     /locus_tag="IWK54_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9592795.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(12698..13738)
                     /locus_tag="IWK54_000649"
     CDS             complement(12698..13738)
                     /locus_tag="IWK54_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9592796.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(14015..15817)
                     /locus_tag="IWK54_000650"
     CDS             complement(14015..15817)
                     /locus_tag="IWK54_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9592797.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(15831..16583)
                     /locus_tag="IWK54_000651"
     CDS             complement(15831..16583)
                     /locus_tag="IWK54_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9592798.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(16592..16954)
                     /locus_tag="IWK54_000652"
     CDS             complement(16592..16954)
                     /locus_tag="IWK54_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9592799.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(17130..18002)
                     /locus_tag="IWK54_000653"
     CDS             complement(17130..18002)
                     /locus_tag="IWK54_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9592800.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(18028..18540)
                     /locus_tag="IWK54_000654"
     CDS             complement(18028..18540)
                     /locus_tag="IWK54_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9592801.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(18678..19508)
                     /locus_tag="IWK54_000655"
     CDS             complement(18678..19508)
                     /locus_tag="IWK54_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9592802.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(19524..20132)
                     /locus_tag="IWK54_000656"
     CDS             complement(19524..20132)
                     /locus_tag="IWK54_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9592803.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(20125..20442)
                     /locus_tag="IWK54_000657"
     CDS             complement(20125..20442)
                     /locus_tag="IWK54_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9592804.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(20598..21326)
                     /locus_tag="IWK54_000658"
     CDS             complement(20598..21326)
                     /locus_tag="IWK54_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9592805.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            21485..22366
                     /locus_tag="IWK54_000659"
     CDS             21485..22366
                     /locus_tag="IWK54_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9592806.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(22603..23646)
                     /locus_tag="IWK54_000660"
     CDS             complement(22603..23646)
                     /locus_tag="IWK54_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9592807.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(23649..24317)
                     /locus_tag="IWK54_000661"
     CDS             complement(23649..24317)
                     /locus_tag="IWK54_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9592808.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24459..26795
                     /locus_tag="IWK54_000662"
     CDS             24459..26795
                     /locus_tag="IWK54_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9592809.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(26910..27221)
                     /locus_tag="IWK54_000663"
     CDS             complement(26910..27221)
                     /locus_tag="IWK54_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592810.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            27402..28877
                     /locus_tag="IWK54_000664"
     CDS             27402..28877
                     /locus_tag="IWK54_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9592811.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            28924..29508
                     /locus_tag="IWK54_000665"
     CDS             28924..29508
                     /locus_tag="IWK54_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9592812.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            29510..29929
                     /locus_tag="IWK54_000666"
     CDS             29510..29929
                     /locus_tag="IWK54_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9592813.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(30210..30494)
                     /locus_tag="IWK54_000667"
     CDS             complement(30210..30494)
                     /locus_tag="IWK54_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9592814.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(30846..31556)
                     /locus_tag="IWK54_000668"
     CDS             complement(30846..31556)
                     /locus_tag="IWK54_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9592815.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(31553..33025)
                     /locus_tag="IWK54_000669"
     CDS             complement(31553..33025)
                     /locus_tag="IWK54_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9592816.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            33163..33636
                     /locus_tag="IWK54_000670"
     CDS             33163..33636
                     /locus_tag="IWK54_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9592817.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(33789..34340)
                     /locus_tag="IWK54_000671"
     CDS             complement(33789..34340)
                     /locus_tag="IWK54_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9592818.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            34445..34975
                     /locus_tag="IWK54_000672"
     CDS             34445..34975
                     /locus_tag="IWK54_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9592819.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(35458..35901)
                     /locus_tag="IWK54_000673"
     CDS             complement(35458..35901)
                     /locus_tag="IWK54_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9592820.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(35917..37077)
                     /locus_tag="IWK54_000674"
     CDS             complement(35917..37077)
                     /locus_tag="IWK54_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9592821.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            37584..39284
                     /locus_tag="IWK54_000675"
     CDS             37584..39284
                     /locus_tag="IWK54_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592822.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(39386..40219)
                     /locus_tag="IWK54_000676"
     CDS             complement(39386..40219)
                     /locus_tag="IWK54_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9592823.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(40523..42688)
                     /locus_tag="IWK54_000677"
     CDS             complement(40523..42688)
                     /locus_tag="IWK54_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9592824.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            42948..45242
                     /locus_tag="IWK54_000678"
     CDS             42948..45242
                     /locus_tag="IWK54_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9592825.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            45235..45900
                     /gene="pgmB"
                     /locus_tag="IWK54_000679"
     CDS             45235..45900
                     /gene="pgmB"
                     /locus_tag="IWK54_000679"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9592826.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            45893..46900
                     /locus_tag="IWK54_000680"
     CDS             45893..46900
                     /locus_tag="IWK54_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9592827.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            46922..47941
                     /locus_tag="IWK54_000681"
     CDS             46922..47941
                     /locus_tag="IWK54_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9592828.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(48222..49589)
                     /gene="nhaC"
                     /locus_tag="IWK54_000682"
     CDS             complement(48222..49589)
                     /gene="nhaC"
                     /locus_tag="IWK54_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9592829.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(49699..51120)
                     /locus_tag="IWK54_000683"
     CDS             complement(49699..51120)
                     /locus_tag="IWK54_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9592830.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(51363..53240)
                     /gene="tdc"
                     /locus_tag="IWK54_000684"
     CDS             complement(51363..53240)
                     /gene="tdc"
                     /locus_tag="IWK54_000684"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9592831.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(53531..54787)
                     /locus_tag="IWK54_000685"
     CDS             complement(53531..54787)
                     /locus_tag="IWK54_000685"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9592832.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            55367..56668
                     /locus_tag="IWK54_000686"
     CDS             55367..56668
                     /locus_tag="IWK54_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9592833.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(56737..57336)
                     /locus_tag="IWK54_000687"
     CDS             complement(56737..57336)
                     /locus_tag="IWK54_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9592834.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        17479 a        11771 c        10311 g        17893 t
ORIGIN      
        1 atttttcagc aaagcaattg cctggtatgg ttttagctcc agactttgat gaagaacagt
       61 atcggaataa ttatcgataa gaatattggc ttctgtgaat ccttctggga tggcgatcct
      121 tgtatcttta tcatagaaat ttgttaatac aagcagctgc tgtccttcaa attggcgaag
      181 aaagccataa acttccggat gatttgcttc ataagcatga taatctcctt gtgcaacgat
      241 tggatatgtt tttctcagcc gaatcaattt cttgtaatag gacaaaatag aatcttcaga
      301 agatagttct ttttctacat taatctcttc tagcttgcct ccaactttta accaaggttt
      361 ccctgtagta aatcctgctt gttctgtgtc tgaccattgc atgggtgttc gagagttatc
      421 gcgagatttt gctttgatcc gacgaaacgc ttctttttct gtgaatcctt gttcaaggag
      481 taacttataa gcattcaaac tttctacgtc aatatagtcg gaaatcgatg aatagtctgg
      541 gtcgatcatg ccgatttctt ctcccatata aatatagggc gtcccacggt tcaaatggat
      601 catggttgct agcatctctg ctccttgttt tcgatactga tcgataacta caaatcgatt
      661 gagcgcacgt ggctgatcat gattattaaa aaatagagca ttccagccgt taccctcact
      721 catattttct ccccatgtat gaaatagtcg ctttaattct gcaaaatcaa atggaggagt
      781 agaccattta ttcccatttt cataatcgac tttcaaatga tggaaattaa agaccattga
      841 taattcttct ctactaggtt ctgtatacaa gatacaattt tcgatcgtcg tagagctcat
      901 ttcaccgact gtgatgatag tggaatccgt cccaaacgtt gcttggttca attcttttag
      961 atacgtatgg gtgattggtt tgtccgtata ttcaggtttg ccatcatttt ctttattatt
     1021 tttcaattgt tcgtctttac cgataacatt gataacatcg aaacggaatc cttttacccc
     1081 tttttccatc caaaaacgaa caactttgaa tagttcctct cttactgctt ggttccgcca
     1141 gttcaagtca gcttgtgtcc gatcatataa gtgcaaataa tatttccccg tatctccaaa
     1201 tggcgaccaa gcacttccac caaattttga tacccaatcc gttggctctt ctcttaaaat
     1261 gaagaagtcc tgataatgct ggtcccctgc tagtgctttt ttgaaccatt catgttctgt
     1321 cgaaacgtga ttcagcacca tatcgagcat gatttctatc cggtatttct ttgcttcggc
     1381 tattagctgt tcgaattcct ccatcgtacc aaagatagga tcgatggctg tgtaatcaga
     1441 gatatcataa ccgttgtctt tttgagggct tggataaata gggtttagcc aaatcatgtc
     1501 gatgcctaga tcattcagat acgggagttt ttctttgatt ccttttaaat ctccaattcc
     1561 atcaccatta ctgtctttgt aagatttggg atagatttgg taaatgactt tgtctttaaa
     1621 tgtcatggat atttacctcc attcaactga tttattacct accacagctt atatatacaa
     1681 gttatcattt attattataa ctgaacagaa agcgtttgta aaatttatct ttatacattc
     1741 taagaggtct gttgcccaat aaaaaacaag agatcagaca tgtatttgtc tatcctcttg
     1801 tttaaaatag caaaatattc ttgtttcttc tataaatggt gttcaaaagc ttttatctac
     1861 ggcaatcagt cttctcaaat gtaaaacatg aggagctaat tttatttgag tattcttttt
     1921 gctcgatgca ggacggcttt tttacaacct ctgattcatg gtgttcaatt agcagctgac
     1981 cctcgttatt agctcaaaac gctgatttca caacctctgt ttttattcat tcgatttcaa
     2041 cgcatcaatc atatcgatat gctttaattt gaaatgggta acaatcatca caataaccgt
     2101 aaatacgata gttaatcctg ctgataagac ataagcccac catgtgatca ctaatggaaa
     2161 gatgacattc tccatagacg cttgttgcaa gatgaagtca gtcaatatat atcctacacc
     2221 aaatcctccg atgattccta aaagagtaaa gatgatattt tctcgaacaa tgtacatcgt
     2281 gacttcttta tcaaaaaatc caaggacttt tattgtagat aattcgcgta cacgctcgga
     2341 aatattgata ttagttagat tatacaagac gataaaagcc agcagtcctg ataatacgac
     2401 aaagattaag acaaccgagt ctaagttcgc cattgattct tcttgcgttt cgatttgttt
     2461 ggacatgaaa gacgtatttg tcacagcgtc tgtggcaagc agtttctcgg aaagcatttc
     2521 ttcttctttt gttgtcatct tatctgtacg cagcaaaaat gcattatcca tcatcgattg
     2581 atcagtaact gaagcataat attcagggga catataaata aagctaccta gatagttttt
     2641 cacgatttct tttacttcga ctttccattc tttttgatct tcatcataaa gcgaaatatg
     2701 atctcctgta gataagtcaa atagttctgc tactttttgc gtaatagcga ttcctgtttt
     2761 aggcaaggag cgtgggttct cttcaagatc agttaaatgg ataaaacgat ccaaatcagc
     2821 tgttttactt ggtacgatca ctgtcagact ttgcgaagca tgtccttttt ttctcaattc
     2881 tacggtttga gtatgaatag ctaggatctc atttactttt tcttgcttct gcagcatatc
     2941 cgctgctttt tgacgactat cttccgtgga tgaatccaat gtaacgattg cttgatagtc
     3001 aatgatcggt ccaaattgtt tggctgaaac agaacttaga gaatctttca atcccacgcc
     3061 tgcaaccatc agcccggcac atccagcaat tccgataatc gccataagca ttcttgactt
     3121 atatcggaag agatttcggt aactgatttt ttgattaaag ctaaggcgtg accacagcgg
     3181 agtgatgtat tcaagaaaaa tgcgtttccc tggcttcggt gctttcggtt gaagcaatgc
     3241 agcaggccgt tctcttaaat ctttgaataa aacgatcatt gcagcgccaa atgcggcaat
     3301 gaagaatgcg gcagctgctt gtaaaatcgg cggccagtca taaaaaataa gcgcactccc
     3361 aatatcatac cgctcgttag acagctcaaa aatgatcctt ggcaaaagat tcgttcctaa
     3421 tatcgttcct aaaacaatac ctatccctga ggctagactt gcatagattg catatttttt
     3481 tgcgatttca actttcgtat aacctaacgc ctttaatgtc ccgatttccc tgcggttttc
     3541 ttctaccata cgtgtcattg tcgtaaaggt gatcaatgcg gcaataaaga aaaagaaaac
     3601 aggaaaaaca ttggcaatcg cagcgatacg atcagacaga tcaccaaatt cttgaaaacc
     3661 gacattttct gaacgttcat tgtataggta tgatggttta gttaaatttt caattttttc
     3721 tgcatttcga ttcaattctt cctctgcaga ggccagttca cgttctttct caactaattg
     3781 cctttcttgt tcagcaagtt gagcttcttg ttgaggtaat gtttgaacag cgattgttcg
     3841 ttgcgcttca ggaagctgct ggatagttgc tgactgttgc gctaactgtt ttttagcatc
     3901 ttctaaccgt gttcttgctt gcgttgtttg tgctttgcca tccgctattt tttgctgtgc
     3961 cggttctaat tcttcgttgg ccgatttttt cagctcttct aagcgttctt cgggtcgatc
     4021 ttgtaaatac ttttctgcct tttcttgatt ttgctccatt ttatctttgt aagctttgct
     4081 gtacgtattc aaattcttta cgtttgtaaa atcaagatat aagacagaat aaatatctgt
     4141 tttaaaaacg gattcaggta aatagacaaa ataatcgacg ctaccatttc cgacgttcgc
     4201 gtatcctctc tccaaattgt tgatgaacaa tggagaacgg acaaagcccg tgatggtata
     4261 ctctttttct ttcaattcat ctgaatcaat ctgataaata tcaccgattt tatacccttc
     4321 atcttccgct ttttcatcca atacaagctg gtttggtttt tccgggagtt ttccatcagt
     4381 taattgtagc ttgttctgct tgttattttt gttataagaa aatatttgaa tcacttcgtt
     4441 tttgccagta ttggaataat agatctgttt tcctgtttct acttttgcat ctgggatctt
     4501 ttctgcttga gcaatatctt tttgtgtcaa gccaccagta gaaacgattt gcaaatcaga
     4561 aagtcctgct gtaccaacat agtgatccat cgttttttgc atgacaggtc cagcagtttt
     4621 gacacctaca aataaaagtg ttcctagcat gacaataagg atgattgcaa tgaatcgtcc
     4681 tttagattgc cgaatttcgc gaaaacctgc tttaagatat gttcttgttt tcataaaatc
     4741 cccctaccat acaatctcat caatggatga aggatgatca tttaattcaa cagaccttac
     4801 tttagcatca ttaatgtgaa tgactctatc agcgatgggc gctaaagcag aattatgtgt
     4861 aatgatcaaa acggtattgc cgtgttgtct gctagcgttt tgcaataatt tcaatacttg
     4921 cttgcccgtt tcaaaatcca gcgcgcctgt cggttcatca cataaaagaa gtttgggatt
     4981 tttagctagt gcacgagcaa ttgaaaccct ctgctgttcg cctcctgata attgggacgg
     5041 aaagttattc aatcgatgag ctaggcccac ttgcctcaat acctctacag gatcaagcgc
     5101 atcaggagaa acttccgttg ccaattccac attttctttt gctgttagat taggaacgag
     5161 attgtagaat tggaaaacga accctacatc tgttcgtcgg taagcagtaa gctgtttatc
     5221 tgaaaattgc gcaatatctg tgtcatcaat aatgatctga ccttcatcag gtgtatccat
     5281 cccacctaaa atattaagaa tcgttgactt tcctgctcca cttggaccaa gtataatcac
     5341 taattcgcct ttttcaattg aaaaagaaat atcgtcattt gctgtaatgg tcgtttctcc
     5401 cattttgtat cgtttgtatt cattttttac ttcgacatac ttcactctga ttcctccatt
     5461 ttcacgatat ttctcgctat atctatgcat cgaatgcgtt ttctcacact tatttatttt
     5521 accactgaat ttctctagac taaagaaagg aatcctttaa tttagttgac aaataaacga
     5581 tttatgattt acttacataa aaaacattgt tatttgaaag aaggaacatc atgaatgatt
     5641 taaaggaaaa tatgattttt gatttatcaa aaactgcttt tgtcgcgatc gacatgcaaa
     5701 aagagatcgt aaataacggt acactctctc cacacactgc agcatctatt ctgacaaaca
     5761 atgatttgtt agtaagaaca ttgaaacgta ccgctgcctt gaaagtttta gtgaacgtag
     5821 acatctccac tttcccttat ttgagtcaac aaactgatat gggtggaatg actgcttctg
     5881 tacctccaga attcactgat ttacttttga aggattcttt gaaagataca gataatatgt
     5941 tgactatcaa aaaatacaat ccaagtgctt tttttggtac ttctcttgat cttcaacttc
     6001 gccggcgtgg aatcgaaact atcattttaa gcggtgtagc gacaacaaac ggtgtatatg
     6061 ctactgcatt agatgccttt caacacgggt accatatcgt gcttgctgaa gatgcttgca
     6121 gtgatcgtga caaagaatcc catcaattgt tcataaaaaa gatatttccg aaaactgcac
     6181 gagtacgctc aacgaaacaa ataatcgaag ctatccaaca aagcaaataa gtactggcac
     6241 acgtgatttt catttacttc tttttgattt catgggaaaa taaccacagg atttagatag
     6301 gagtatgaac atggtgaaaa aaaagatcac aatcaaagaa gtcgctaaac tttccggcgt
     6361 ttcaatcaca actgtctcct tgatattaaa tggaaatacg gagaaattca gtccgaaaac
     6421 tgttcagaaa gttttagctg ccaaagaaga attaggctat cagcctgact attttgctag
     6481 acaaatgatt accaaggaaa cgaaaacaat tggtgtactc gttcctgata tcacgaatcc
     6541 attcttcagt acactgatgc gaggaattga ggatatcttg tataagcaga atttcgtcac
     6601 gatattatgt aatgctgatt ctgatcatca aaaagaaatc gaatatctag ccgagctcac
     6661 acgtcgaggt gtggatggat tcattatcgc gacttctgct gtctctacag atgcaatcaa
     6721 cgaaaatttg aaaaaacaag gccggccctt cattgttttg gatcagaaaa aatctgaagg
     6781 atttagtgat gccgtacgaa cagacgattt tcgcggtggt tacctagcgg gcatgcactt
     6841 gcttagtcta ggacatcaaa cgatcgccct tgtctatcct gaaaatccac cggaaaatgt
     6901 ccacgctcgg attgaggggt ttaaatctgc tcttgatgtt taccaaatcc ctcacgacca
     6961 actgatcctc ttacctacac agttctcaaa acaaggtggc taccagatca cagcagaact
     7021 tcttgattct gctgcaactg gtgtgtttgc tttaaatgat gaattagctt ttggattata
     7081 tcgcggatta gaggaagctg gaaaaagcat ccctgaagac tacagcatta ttggctatga
     7141 taatatagat atgtgcgagt atatcaagcc gaaactgaca acgatcgcac aaccaatctt
     7201 cgaattggga caaacatctg ccaagttatt gttagatcgg atccaattcc cagaaaaaga
     7261 atgggaagaa aaaagattac ctgtacgatt agaaaaaaga ttttcaactg cacccttgaa
     7321 atagctttca aggtgtgcta tatttttatc gaactattaa aacgttttac taaaacgttt
     7381 taaaaaataa ttttcccatc aaaataaatg agatagttgg aggtttgtaa aatgaacacg
     7441 gttacagtaa ttggaagtat taatatggac actacccttc gtttaaccaa catgcccaag
     7501 ccaggcgaaa cgatgcatgc gcatgagatt tttcacgctg gcggaggtaa aggtgccaac
     7561 caagcagtgg cagccaaacg ttcaggcgca cggacatctt ttattggagg cgttggggct
     7621 gacagcgaag gacaacagtt attagatcta ctgacaaaag aaaacatcga tacatctggg
     7681 atagctgaaa tccaaggcgc cacaaccgga caagcaatga ttatggtaga cgcagcagga
     7741 gaaaatagta tcttgatcca tgcaggggca aataatgctt tccatgaaca agaagtccta
     7801 aaaaacaagc aactgattac aaacagtgac tttattattg ctcagtttga aagtagctta
     7861 gatgcgacga tcctagcttt ttctattgca aaagacgcag gaaaaacaac gatcctgaat
     7921 cctgcaccag ctcgtgaaac gattcctact gaactacttg aaaagacaga tatcattata
     7981 ccaaatgaaa cagaaacaga aatcattaca ggaatcagag tgacagatca taacagtttg
     8041 gtcgcagcag ccgaaaaact ccatgaatta ggtattggaa cagtaatcat tacactaggc
     8101 agtgccggcg ctttctatca tacagaaaaa gaacacggta tcgtccctgc ttttaaagtg
     8161 gatgccgttg acacaacagc cgcaggtgat acatttattg gcgcattaag cagcacactt
     8221 cagccagacc ttagtaactt gaaagaagcg atcctttatg gaaatctagc ctcttctgtt
     8281 gctgtgcagt cttatggtgc gcaaccttct atcccttacc gcgaagcttt agaacaatca
     8341 gcagtctaaa attaacaatg agaggaagaa acaaacatga aaaaaggaaa agtaatcaat
     8401 tcagatattt cacgagtaat cgcacaaatg gggcactttg ataaattaag tatcggggat
     8461 gcaggcatgc ctgtcccctt tggtactgaa aaaatagatt tggctgtctc aaacggtctt
     8521 ccaagttttt tatctgtttt agaaaacgtt ttagaagaac ttgaggtaca atcgattgaa
     8581 ttggcggaag aaataaagac aatgaacccg gaaatattgg aaaaaatcaa attattgtta
     8641 ccagatacac cgattagctt tatcccacat gaagaaatga aaaaagagtt gaataactgt
     8701 catgcattta ttcgtacagg cgaaatgaca ccttacgcca atattatatt agtgagtgga
     8761 gtggtcttct aatggaaacg acaatgaatc ctgaacagat ggtactgtcc gagcagattc
     8821 atatgctgat tgaacagttg ggaggaaaag acaatattga ttctgtcctt ccatgtgcga
     8881 ctcggcttcg tatcaaattg aaaaaacagc cagtagccaa tgcagaaaaa ttaaaacagc
     8941 aaatcccttc cattaagtat ttcattttac tcgatagata tgcagaaata atcatggatc
     9001 agggattggc tcctgcttac aaggaattga cgcgtcaaat cgatcattca gctccgaaag
     9061 aaaagctaaa tccgatcgaa aaactatttg gtttgatatc ggaaatcttt acgccgatca
     9121 ttccgctgtt tgccggttct ggtatattaa aaggtctggt tgttttagcg actacgattg
     9181 gctggcttag tgaaacaagc ggaacttatc atattttgtc tgctgcgtcg aatgccgtct
     9241 tttattttct gccaatcttt cttgcttttt ctgctgccaa aacgtttaaa gtgaatggat
     9301 atattgcggc tgtcattgct gcagcactga ttgaaccgaa tctgacctct ttactttcag
     9361 acagtggcaa agcagctact gattttctgg ggattcctgt agtactgatg cagtatacgt
     9421 ctactgtcat gccggcgatt ttaggtattt tcttttattc ttttgttgaa aaattcctca
     9481 aaaaatatat tcgcgaaaat atgcaattag tatttgtacc tttactttca ttagttatca
     9541 cggttccttt gacgttaatg gtattcgggc ctgcgggtgt ttaccttggc gaaggtttag
     9601 ctgccgcaat tacttggatg atggatatta acggaccgat cagtggcgca atcatcgctg
     9661 gtggttggaa tattttggta atatttggta tccaatgggc tgtaaaccct gtcatgatca
     9721 gtaatatctc tgcttatggg ttcgaccgaa tcgttccgtt gacaggagca gccaattttg
     9781 gaatggctgg tgcagcttta ggtgtatttc tgcgaagtaa acgttcaaaa acccgttcga
     9841 tctccggttc tgcttttgct tcgattttac ttgcgggagt gaccgaacca actgtttacg
     9901 gaattgcgat cccattgaaa aaaccatttg ttgctgcttg cattggtgca gctgctggtg
     9961 gagctgtaat gggatttgcc caagtgaaag cgatcgcttt tgtatttggt agtctgacca
    10021 cattgcctgc attcatttcc ggtacttttt tctggtactt agcaggactg gctgttagtc
    10081 tagtcgtagc aatgatcact actctcgtta gcgggtttga tgaagaccta atgtcttatg
    10141 aatagttaat gaaaaagact gaagtatgag atgtttatca catatttcag tctttttctt
    10201 tttaggatca ttatttttat aaatttttgt gatactttcc aaactttggt ataatttaca
    10261 tatgcataac catttttatc cccacttgtt tatccccaaa caaaggtggt ctatgcaata
    10321 aaatgaacgt caaagaaaaa cgaatgaatg ctcgttgctt atttacaaat tgttttattt
    10381 agaatacaca gacagattta tgaaaagaaa aagcttttcg acacgattat ctgttccaaa
    10441 aaatgtctaa tgaatgctga ataagacaga aaatcaatga aaggaaaagg actagtttat
    10501 tatgaaaaat aaaaaaacga tacgtttgct agctgctact tggctgattg ctccgtctgt
    10561 tttgtcgacc aataccgtat ttgccgtaga taatcaaata gctgatcaaa cagtcacaac
    10621 agatacgact acatccagct cagctactag tgaaacaacg gattctttta cagaagaccc
    10681 aactggaaca actaactcaa caacaggatc gaccgagcca actgaaattg atccggctga
    10741 tccatcagaa aatgaaaagc cagccgagac atattccctt acaattgctc cttctttgat
    10801 tggtaagatc caactgactg cttcgacagc ggattcccct gaacagcaac ctttggatat
    10861 tacggaaagt ggaacggtag aaggtttatt agcggaaacg aaggtaactt acactattac
    10921 gccagctgaa ggtgaacaaa ttgcttcagt cgctgttaat ggtgccaaag aagtaaatta
    10981 tgatgcaaag acctttacag taggtacgaa caatttatct attactgcag aatttaaagc
    11041 tggaccggat acttcaacag agaaaccaac tgatcctagt gaagaagtcc ctagtacacc
    11101 agatccggaa acgccaaaca aaccagatgt gggtaataac aacaataata atggttcaaa
    11161 taattcaaac aataataaca ataattcgaa cagcgggaat tcttcgaatc aaacaaaccc
    11221 atctggatcg aataacaatg gaaatactca gagagaacca aaccatccaa acaatggttc
    11281 atcgaatggc ggaacgaaca atgcttcatc tcgtccaaat acagcaaatc aaagcggtat
    11341 agagaatcca tcttctgctt cttctgattt tgttgtaaaa tcaccaattg atgctgtatt
    11401 gcctacaaat acaacgaatg tccaacaagc aatcgttcga gaagcattca agcatcttgg
    11461 aaaaccatat gtatggggag caaaaggtcc aaatactttt gactgttctg gtttgactta
    11521 ttacgtatat atgaaagcaa ccggtcatta tatcggtggc tggacaggcg aacagcaata
    11581 tgcaggtacg cagattccag taagtcaggc ccaacctggc gatctcgtct tctggggacc
    11641 ttcttccggt gttactcacc atgtaggtat ctatattggg aacggacaat ttatccatgc
    11701 gcctcaacca ggagataaag ttcgagtaac ttctatttca gatttcacgc ctgattttgc
    11761 tgtccgagta aacttagcag gtttacctgc tgcttcaggt tcgctgacag gcggctcatc
    11821 tattttagat ggattagatg gctcgttcca tttcagtcaa aaccaaacaa ctgatcagtt
    11881 cttgaaaaag attgcagatg atgcacagga aatcgggcaa aaagaaggaa tctacgcatc
    11941 agtcatgatg gctcaagcta tccttgaaag tggatctgga aacagcttat tatcaagcga
    12001 accaaatcat aacctttttg gtatcaaagg atcatataaa ggaagcagcg tgaccttcaa
    12061 tacacttgaa caagatagtt ccggacaaag ctaccaaatc cgcgcacaat tccgtaaata
    12121 tccttcttac aaggaaagtt tggaagacta tgccgacttg atcaaaaatg gactgactgg
    12181 caatcctgat ttctacaaac caacttggaa gtcggaaaca aaagactaca aagaagcaac
    12241 caaatatttg gaaggacgtt atgctacaga tcgccaatat tcacaaaaat tgaacgcgat
    12301 catcgaagcg tacgacctga caaaatatga tgaaccgaaa aaagaagcag caacaactga
    12361 agaagcaaac gctgaaatcc tcaatatgtc taagcgattc gatgtgccaa tcaaatggac
    12421 aacagatggc gtgccaatca caggattaga attttcaacg ataaaacgcg tcaacaacgc
    12481 ttcttccatc ttccgtgtcg cttcaatctg ggatttgtgg aacaatttta cggcaagaca
    12541 gataccagaa acaactactc gtacagttaa agctgcaact gttcctgtac taaccttgtt
    12601 aagtttagac aaatcattct ggcttgatct gcctttaaag aaatagaata aattaaaaag
    12661 gggatgtgac actgtcacac tcccttttta atttattcta tttctttata tttattttgt
    12721 attccgttac aaactcgcgt acttggttag gttccagagt gacatctcca aaactttcaa
    12781 gtcgctccgc tcctggcagt tcttgagtct caaatgtgat accgccatga tttactaatt
    12841 tattcccgcg catttccggt ccatcttctc caaaatttgc tgtaaaaatc acgattcctg
    12901 aagcatctgt ttttacttgg atcgatactt tttcatctgg gcttgtcaat tccgcagcat
    12961 ttagtaaacc agattgtttt ttcaacaaaa atggatgatc gatgccgccc actatcattg
    13021 attggcgaaa atcactggca aagacctcac ctaatttttt aggacgttgg aagtcaaatg
    13081 ctgtgccact tactgaaact acttctcctg taggcgttgt atctttattg agcgtagcaa
    13141 ataatcggct attcaaccaa agtatatgtt cgtcgattga ctgtgctgga tcaccggtta
    13201 aattgaaata gacatgattt gtaggattga atactgtttt gcggtcactg atcccttgga
    13261 ccgtcactcg ccatgtatca tcgttcgtca gcgtataccg tacttctaca tctaaagttc
    13321 caggaaatcc gtttcccata tctgggctag tctgatagaa aatgaccgat gcttcgtttt
    13381 caccattgat gactgtataa tgccactgct tggcttcaaa gctaggtgca ccaccatgta
    13441 aacaatgacc agtgtccggg tctgttttta cttcgaacgt ttcatcatct aatttgaatg
    13501 tgccattcgt aatccgtcca gccatacgtc cgattgaagc accgatataa gggtcttttt
    13561 ctagatattc ttttgcttca tcaaaaccta agatcaactc tcgtttttca ttatcgatta
    13621 ttgtttgaaa atgaacaacc cgtgccccaa gatcactgac agacaaagat gtcccatttt
    13681 gattcgtgat cgtaatcaag tgatagtttt cgccaaaatt ttgttcattg atttccatgt
    13741 tccttgcacc ctcttttctt tcatttgcac tgattccagt atacatggaa accgttttct
    13801 taacagctta ttccgtgagt aacgagaaaa aatttactat attctaataa aagaccgtga
    13861 aaaaaaggtt gtgacaaaaa tattgtcaca acctcttttt ccgaacaaac ggtgttcaaa
    13921 agcttgcatc tgcggtaata agcccaacca aacgaaaaat atgaggagct atttttgctt
    13981 ggctgttctt attacttgat gcaggacagc tttttcacaa tctctttttc aatacgattc
    14041 cagtatataa ccatgtcgtc agtacatagt agatgaaata caatacgaag aagatcgtaa
    14101 acggaaccca aatatctttg tatggatttt gcatgaatag accaaacatt tgtaatccga
    14161 ataagacatg gactacacct aagattcctg gtgctaagaa taacacgcct acttctctac
    14221 ggatcgattg tttaagcaac ccgcgtcgtg ttcctatctt ttcaagcatt tcataacgag
    14281 caatgtcact cttgcttcct gacaagatct tgaacatcaa acagctagca agcatggtca
    14341 agaaagctaa acctaggaag aatcccataa attcaaaacc agagaataga ccattataca
    14401 cttcatacat ttcatatttc tggttgctgt actctacttt ttgcaaagat ggattgttca
    14461 ttttattttg agcaacaagt gtttcgattg gtttcaggtt tgcttggaag tcttttacct
    14521 gaactagctg taaagtggat tgtggcaagt tcagttgatc aaattctgat tgactgacta
    14581 ccttgatttc tttttcttgc tgttccggca attcatagct tcgaagttcc tcttgtacag
    14641 tcagttcatt cattttttta gcaggaactt tttctttcgt gatcttcggc ggtgttccat
    14701 cttcatgttt tatcatgatc aatggttgag ccgcaaattc acttgcatta tagtaaatcg
    14761 ttcccgcatc ttctttttga gtataagaag cagaaagtgt tggtgaaagt tctttgattt
    14821 tttgctgatc ttctgcccga ggattattca tcactagatc atatccagaa ttgttttctg
    14881 tcaagtctgc aatttcattt ttaaaaccta atcctaccgt caaagcgccc agtgccaagg
    14941 caaacagcat agcgaccatt gagagcattt gtgtatattc acgaatacga aagtttaact
    15001 gtgacaatgt gaaattgttc aattttttca atgaaatctg ttctgatttc tttagtaatg
    15061 ataaaaagaa aatgatcaca gaatggaaaa tgaaataagt tcctaatatg atcgtaacta
    15121 aggctacccc tataccaaag acttgatagg tccccacgtg tttcatcata tagtaaccgc
    15181 agcctagaaa agcaattccc agtactactt caaggaaaaa caagaattta tgctgcttga
    15241 ttcttgttgg tgtctgatcc gcacgaatca atgctaaaat cggttttttg acaatcgaaa
    15301 aagcattgac aacagcagct aataaaaaca aaacagcaaa aaacaaaaca gtgatcaaca
    15361 acccgtgaat actaaaaggt gtaaaatgcg aaatattgag atctaactga tccactaata
    15421 atcggtggac gatacttgtc aatcctacac cgacagctga acccactaaa gtagctgcga
    15481 tccctactaa gaatgtttca gaaaaaatca tctgtgcgat ttttctgcct tttgctccca
    15541 gcatcataaa cattgcatag tctttttgcc gcatcgtcat caaaaaggaa tttgcatata
    15601 aaatataaac gaatgtgatg atccctagta aaacagaacc gaatcggaaa atgataactg
    15661 tagccgcaac gatcgtatta cttttcaaaa aagcttcgtt gcttgccatt gattcaaaca
    15721 tgtaaaaaat agcagatgcc ataactaatc ctgagaataa caccaagtag tctcttaaac
    15781 ggctcttgat cccagtaagt gataatttcc aaagcatagt caatctcccc ctactgttcc
    15841 aagtttccaa gaatagatag tatttctcga tagaaggaat cacggctttg atctccacgt
    15901 ttcacttctt gatggatacc gccatccttg atgaataaaa tacgttggca ataacttgca
    15961 gaaaacggat catgcgtcac aagaagaatc gatacatgat cttttgtatt caattcgtcc
    16021 atcgtatcta aaagatcccg ggcacttttg gaatccagcg cacctgtagg ctcatcccct
    16081 aatagaatcg ttggttgtgt gatcaatgca cgtgcggcgg cgacccgttg tttttgtcca
    16141 ccggaaatct cagaaggata gctttccaaa atatgagaga tggataaacg ttctgcaatt
    16201 ttttggacac gttgcttgat ttcttttggt ctgacgcctt gtaaagaaag aggaacagcg
    16261 atattttctt gtgctgtcaa gctttctaat aaattgaaat cttggaaaat aaagccaatc
    16321 tcattcgcac gaaaatctgc tagttgattt ccttttaatg tggtcacatc tttttgattg
    16381 attcggatat gcccatctgt tggtttatct aatgtggata aaatattcaa aagtgtcgac
    16441 ttaccagaac cagaagcgcc catgatccca ataaattcgc ctttttctac atcgaagctg
    16501 atgccgctta atgcttgcgt ctttttctca ttgttttttc cgtatacttt tgttacattc
    16561 tgtacttcga ctattttgtt catttctttc cctacccttt aattagtttt tccagtgctg
    16621 ttttcggcac gtcttttttt tcgacttctt catacgaagt gacaccttta tttttgttcc
    16681 aggtgatttt taaatatgct cctttacgaa gcggtcgagt caattgtccc ttgaagtcca
    16741 gttctttttc cttaccctct ttcgtatagc caggcaggat atatctatag ttgactgttt
    16801 cttgtccaga gtctgatttt gaaactgttt ttgtaccatc tgtcgtaatt tgtacatagt
    16861 attcttctcc acccataact acgtaatctg cgattttcac ccctgcaagt accacaacga
    16921 tgaaaacaag tagaccgatc agaagttttt tcactttttc cccttctctc ttactttata
    16981 tactgattat agaaaaaagc gaacaattct gccttcgaat caactatcat tcttctttca
    17041 attttgtcat tttctttaaa acaactaaaa aaagagttca agacaataat ccgtcctaaa
    17101 ctccttcttc ctatcttaca aaaagtatgt cacttacggt ctttcatgtc gcctgtattg
    17161 ataacaggtt gcgttccttt gtctgtaatg attgagacaa gtaaattatt gatcaattgg
    17221 actttccgat catcggtgaa attgatttct tgtccatctt cgatttgctc taaggccatt
    17281 tgagtgattg tgacagctcc ttcaacaatg gtttgtcgag cagacaaaat ggcttttgct
    17341 tgttgacgtt ggagcatggc actagcgatt tctgttgcat aggctaaatg gttcaagcgt
    17401 gtttcgatta cttcgacacc tgctactgct aaacgctctt gcagctcttt ttgcaactcg
    17461 tcagacactg ccgtcgtgtt tcctcgaagc gtcaaatcat tctcattgaa tgtatcatac
    17521 gggtattggc tagcgatatg ccgaatggct gtctcacttt ggatttctac aaaatcttga
    17581 taatacgcta catcgaataa tgcctttgct gtatcaacga ctttaaagac tactactgct
    17641 gagatttcga tggggtttcc atccaaatca ttgactttta aaacagaact attgaaattc
    17701 cgaactttca acgaaacagt cattttttgt gccaaaggta tcgtaaggaa aaagccattt
    17761 tctcgaatcg tacctaaata ttgtccaaaa aacaaaatta cttttgcttg attaggactg
    17821 actacagttg ccgaacttaa caacaataac gtgatcaacc aaagaaaaat acttaaaaaa
    17881 agtgcccaaa ctttcgcttg ccacatacct aaatagaaga aaaacaatcc aactaaagct
    17941 aatacgacca atccgacaat ccctgcataa ccattgatat aaaatgctct tttttctctc
    18001 atgctacttc ctgcgctcct ttatccttca ataaatatct aatgttactt ttttaatttt
    18061 ttttcgaata cgcgccatta caccttctag ttctaactct ttgtcttcag tgatgtacca
    18121 accccttgcc tgtgtccctc cggctgcctg cggtaaataa tccaattttg ttcgaaccca
    18181 gtgcttttct tctgtaaaat agtcttctac caagacctct agatccatcc ctgctgaaag
    18241 aggcaaatct tcccgaacag atttaaagat aaatcgttcc ttcttcccat catacttcaa
    18301 tattcctgta tcgataatcg gtaaagaata ataatccagc tctttcttca ccgtcaataa
    18361 atgtttgaaa acttcatggt acacttgctc taattggcga ccttctgaat tgttcgggta
    18421 attaaccatc actttttctt tgtctggatc atagaaggtt tggcttagtt gagtgatata
    18481 agtatgtaat ctttccattt ctgtttttaa cttttctctt tgaccttctt ctaccttcat
    18541 atactctttc accctgtttt ctttttagat attttctttc ttattttact atagttatgt
    18601 gcgttaagcg atgaaaaaca gcaaagattg gaaggatctt tgctgttttt tcttaaaata
    18661 tccagttgag tacaggatca atctgtttca ttgtcctctt gatccgtcgt aatattgatc
    18721 gatgtgtctt tagcagtgat ctttagcttt ttctgtcctt gttctaacaa agtatttgtt
    18781 attggctggt agtctttttc aatataagat tcaccagacg tttggatagt atagccactg
    18841 ttttccatct catgcaaaga taaattgcca ccgtttagga tgacttttcc ttctttgctg
    18901 atcaaactgt cttctagatg gaagttgctg ttttcacctg tgacgatcaa gttatccaca
    18961 ttggcctcat ctaatcgaag tgagcctaat tcatctttcg cagttattgt tcctcctgta
    19021 aattcattga acgataccca gccatcatgt gtcttagtat tcactttttt gattgatact
    19081 tcgttgatat caattgcact attattccct tcgattgtca attcagataa ttcatgatct
    19141 gcagggacaa caatagtgat ttgtgcactt gtgtcttcta aactaatgat tgcacctgtt
    19201 gattttttga ctgcattcac agtcaacacg ccatttttct cctttatttc tggcttttct
    19261 gtcttatcat aagtcgtttt tatctcaaaa tccaatccgc gtatgaccga aactgtttga
    19321 ttttctgcat ggatgacgat tttgtttgca tccttgattt tttttgtctc agtaaccata
    19381 tcgatcaacc gaggaccatt atcccaagtc agactctttt gagcgcctaa tgtataagtt
    19441 gcacctgcta ggaggccacc agtaagcatc agaccaatac cggtaatcaa aaaatgtttc
    19501 aatctcaatt tatgtgtcct ccttcaaacg cccgctgaat gcagatctct ttttgtaata
    19561 aaacgtcgtc ccatccattt aacaaagttc atcatgacat aaaaaagccc ttttgttcct
    19621 ttcagcaaca gtggcgaaat aaatactgcg cttcctgtgg cgaacagacc gacaccgata
    19681 aagaataatc cactcagaat cgattgtcca aacacactga ttcctgagat gatcatgaat
    19741 aggccaacac ctaagatact gatacataaa acatagatac taaaaatgag cgctagtaaa
    19801 gcaaccaata gtcccccaat aaccaatata aaccccagtg ctagtggaat gaggacagga
    19861 gaagcaaata aaccgataat gattaaccaa atcaggcgaa tttgtctttt tggatttttc
    19921 actaactcgt tttctgcttg ttcttcttct gaaaagtaac tgatttttaa ttttaaggca
    19981 aactgctttg gtgtaccata ttcatcaatg atctgatcta atgtttttcc agaatctaaa
    20041 aaatattcct cataatactg gatcaaatca aaacgatcgt cttcaggaat ttcttgaaga
    20101 tgatccacaa cttcattcaa atattcattc atttgtgctc tcctccaata accggtctaa
    20161 agaatctcga aaaacaatcc attcatcaat aatttcttct aaatgacgac ttcccattgg
    20221 tgtcaacgag taatatcgac ggtttctgcc ttcaaaaggt tcatcatagg tctcaagata
    20281 tccttctttt ttcaatcttc tcaaaaccgg atatagtgta gattcactga cagagagact
    20341 ttcttgtacg atttttgtta aggcataccc atacatatct tccttgctta atgctgctag
    20401 cacagtacca tcaagaagaa cagttggaat ctggatgtgc attacgtcac cttctattct
    20461 caagtgtaat attttatttc aatctatatt atacaacata taatatagtt tcaaaatcag
    20521 tttgtcaaat acaaaaaaac tgactaaaag atttgatcct ttttagtcag caaaaaatgg
    20581 cttctctttg atttttatta taacagtggc ataatccaat ttgtccacat ccagctcagt
    20641 ggcatgataa cgatcattgc aggtatcatg tcagctactg ggaacatttt cacgttgatc
    20701 attctaaaac cggtagcgac catcaagaat ccgccgcaag ctttaaaatc taagatcatg
    20761 tctggtgtag tcaatggata aataaatttt gccaataaga acaaaatata aaaaataatg
    20821 aattggggaa ttgctatcac agaaacgaca taccctaaat tacacgcaaa aatagctgct
    20881 gtgaaaaaat caaggacaga tttagaaatc aaaatcgtac tgtctcctgt catcccagaa
    20941 tctaaacttc catagatccc cgtaccactt gcacaaaata gaacaatgac cgtcactaat
    21001 gtgctgataa attcatcgtg ggtcatacca agcttttcac tagggaagat tttagaaata
    21061 ggtacttgca tcagcgcagc ccccttattt atcccattac ctaaatgaac aatcagacca
    21121 atcgctgttc ctatcactaa agcaaaaata acagccggca tatttttcat cggcgcaatg
    21181 gaatagattc ccatccccat cgaacacaca ccaaaaacca tcgtcagctg tgctttaaaa
    21241 tgttcactta atttatttcc tactaatccg ccaattagtc cgccaagcaa aacggaacct
    21301 acattgatca aaactccagt tggcatcaaa aaactcctct ctttttctac gaagcgaaaa
    21361 aaatcagaaa aatttctgta attattgctt tcgctgcaag aaagattgta acagacagag
    21421 aagattacac caattcattt tttgtgttaa agtattcttt attggaataa gtaggaggaa
    21481 tatgatggat atccataaat tcgaagtttt tcttgattta gccgaaacaa tgaatttcac
    21541 caaaactgct gatcgacaat tcaccaccca aggaaatatt tctaaacaga ttctttcgtt
    21601 agaaacagaa ttagacgtca aattattcga acgcgcccat agaaaaatcg aattgaccga
    21661 agctggaagc ttattgctcc catatgtaaa aaatgtcgta gaacaatacc atgctatgca
    21721 agaagtgctt gaaacctata cgaaagacaa caatctttca ttaaatatct taacgatacc
    21781 gacaatgatc aattataaag gtttttccaa aatcaccgag tttttgaaac gccatcctga
    21841 atttacagtc cagttgaaag aagtggaaag tgtcgaatta acttacaatg acgccaatga
    21901 acatagtgac acgatccact ttgctagaaa tttccaggcc ccaccaagtt cgattgagtg
    21961 gttgcctact gaaacagacg attttgtagc agttctacct aaaaatcatc ctttagctag
    22021 taaaaagaaa ttggatctaa gcgaactgaa aaaggagaac tttttgctcc ttggccccac
    22081 tacgaacttg tatcaacctg tactagatct gtgtcgcgaa gctggatttg aaccaaaaat
    22141 ctcttataaa ggagcaagaa tcgacttgat catcaatatg attgcaaatg acatggggat
    22201 tgctattgtt atggaaaaaa cagtcaagaa tctattaaaa gaaaatactg tgattctccc
    22261 tatttctcca acaacagaga gttatcttgc atttactcgt aaagtcggtg agcattcttt
    22321 ggcttcagat acattttggg agtatttaaa tcaagaagaa aactaagccc tacattttct
    22381 gtccatctat ccacccaaaa aaagagagcg acgcatgact ttcgctctct ttcttgcatc
    22441 atgtccttgt ttcaatgaca cctctaacat ttctcgtagt tcaataagaa agcagcgatt
    22501 tttatgccaa aagaaccgta agaacatttc cctaatcaca aaaaaacgac taaaaacccc
    22561 taaaaacctt caatagaatt tttaggggtt ttattctttt tcctattgtt tacttaaccg
    22621 tatcattgct gtcgttccta ctccttcgat actgctataa tcaatacgtc catcatgcag
    22681 ttctagaatt tttttaacca gtgaaagtcc tagcccattc ccagaaattt gacgacttgt
    22741 atctccttga taaaattttt caaacattcg atctctggtc tcttcattca ttcctacacc
    22801 tgaatcggtc acttccagtc caatctctgt ggctgtctca aatagtttga tatgaatctg
    22861 tccattgatt tgattgtact ttatggcgtt atccatgata ttcagccaca cttggtacaa
    22921 aaactcttca ttgcctgtgt aatttactgc agcaagttcg atatccaatt ctagctgttc
    22981 tttctcccat ttaggctgca aaaaaacgat tacttctctg atttgttcat ctaatcgata
    23041 ctctttcttt tccagttgga tacgctggtt ttccagtttg ttcaatttta agatgttctc
    23101 tgttaattgt gaaagctgcg tgatactttc gataatccga tataaaaata tttggcgttc
    23161 ttcgtcagag agatttgggg cttgtaacaa ttgaacatat ccttggattg ttgctagtgg
    23221 tgttttgaat tcatgggata cactggacac aaagtcattc ctcagtgtct cgatactatt
    23281 gagttcttgg accataacgt tgaaatcttt atacagttgc tgtacttctg ctactttctg
    23341 ctgttcatcc attcgaatcg aaaaatctcc cgttgctacc agactcatat ttgtccctag
    23401 cgcactgatt ggatgcagga tacgccgccc aacaaaaatc gatattcctg tacccacgac
    23461 catactgaac cccgcaaaga cgaataacgg gtataacgtt ggttttccag ccgattcagc
    23521 aggcgagata gaatgtcgag acaataagaa aataaaaccg agaaaacaca gcagtgtcac
    23581 taagatcgaa atgaagacaa tactagcaaa atagatccat aattgtgaaa atagcttttt
    23641 tttcatcttt aaataaccgc cttatagcct agtccccgta ctgtgatgat ttgaaaatct
    23701 tccgtctttt caaaacgggt ccttaaacgt ttgatatgta catcgatcgt tcgttcttct
    23761 gtattcgtat ctaatcccca aatatcatcc atcaactgtt ggcgagtgaa gatcttgttg
    23821 ggataagaca gtaatttata cagaagcaga aattcttttt gaggcagaat caacgaatgc
    23881 tgatcttgta acactgtata agcatctagc tctaattttg tttgtccgat tatcaagcta
    23941 ctgctgtaat tgatctttga acgtcgaagc aatgcttcca cacgtaggat catctcattg
    24001 acatcgatgg gttttgtcat ataatcatct gctcctaatt tgaacccgcg tttcttatcc
    24061 tcaaaatcgg cctttgccgt aatcattaga acaggtgtgt caaatctggt gctacgtaat
    24121 gagtctagca agtcatagcc atccattttc ggcatcatga tatctgtaat gattaattct
    24181 atacgtgtcg cttctagttt tttcagcgca tcctctccat tcaatgcggt gatgactgaa
    24241 aagcccgctc gttccaacac agaacggtaa agcaactgta attgcggatc atcttctact
    24301 actaaaatac gattcattga tctccctctg ttttttgaaa aatttctaga ttttttcttg
    24361 tagttcatct tcagttaaca tttatttcgt atactacgat cattgttctg atatacttat
    24421 tagtataccg aatatttcaa tacgggagaa gggtgattat gcttcaatta aaagatataa
    24481 aaaaatatta taaagtcggc gaaaccacca ccaaagcatt ggatggtgtg tctgtcgctt
    24541 tcagacaaaa agaattcgta gcgattttag gtcctagtgg ttctggtaaa acgactatgc
    24601 tgaatgtgat tggtggacta gataattatg attccggtga tatggtaatc aacggaaagt
    24661 ctactaaaga ctttaaggac agtgactggg atgcctaccg aaacaactct atcggtttca
    24721 tttttcaaag ttacaacttg attggtcatc taggcattat tgaaaacgtt gaacttggga
    24781 tgacattaag tggtgtttct aaagacgaaa aacgaaaacg ggcagaagat gcattgcacc
    24841 gagtcggctt gacagatcat atgcataaaa agccaaatca gttatctggt ggacaaatgc
    24901 agcgagtggc catcgctcga gcactagcca atgacccaga tatccttttg tgcgatgaac
    24961 caaccggagc gttagataca gaaacaagta tccagatcat ggaattgatc caagagttat
    25021 cgaaagaaaa attagtgatc atggtcacac ataatcctga actggcccat caatacgcag
    25081 atcggatcat tgaattttct gatggtaaga tcttaacaga ttcccatcct cacatcgaac
    25141 gaccaaaaga tgatcaattt aacttacgca gaacaaagat gagtttttgg acagcgttaa
    25201 agctttcctt caacaatatc cgaacaaaaa aaggacgtac tttcttgacc tcctttgctt
    25261 ccagtattgg gatcatcgga atcgcgatcg ttctgtccct ttcttctgga ttccaaaaac
    25321 aaatcgataa cacgcaagct gaaacgatgg cgaaatttcc gattactatt tctaaggtaa
    25381 cgactaacca gactcgtgac gatgccggat tgggtgcaag caaagcggat tatccagaca
    25441 gcaaaacgat tactgcaaaa gtcagcgaag aagataaagc acagcacacg aataagattg
    25501 atcaaacata cgtcgattac gtaacagaca tcgatcctaa cttaagtaat aatatcggat
    25561 ttacccgtac tacaggaatc aatcttttac gtgatgtaaa tggcaaagta cagcctgtca
    25621 gcttctcgaa tcaaaatcct gatacggaat ctctttcttt atctagtacg atgtcagcaa
    25681 tgacaggtgt gggagtgtca agtttcccta cacagcttga cactagcaaa gaaaactttt
    25741 taaaagacaa ttattcttta cttgccggaa gttatccagc ctcagcaaca gatgtcgtgc
    25801 tgatcgtaga cggcaataat aatactaata tcaacgcatt gaagaatctt ggatttgatg
    25861 tgaaagaaga cgaaaaactt gattttgatg atatcgttgg tacaacattc aaattagtaa
    25921 acaacaatac ttactataca aaacttccaa ctgggaattt tatccctaat acagattatg
    25981 atgcgatgta tcagaatgct tctgatgaat tgaagatctc ggggatccta cgcgtgaaat
    26041 cttcttctac aatgaatctc ttatctccag gaatcgctta tagcgatcaa ttaacgactc
    26101 aaatcgtcaa cgaaaacaaa gaatcagaaa tcgtcaaagc tcaaagagac agtggtgtaa
    26161 atgtcttaac tactgaaaaa gttgacgaaa atgccaaaca aacattgctt agttatttag
    26221 gtggagacag cttgccttca agtatcatga tttatccgaa caattttgaa gacaaagaaa
    26281 agatcttgga ttatctagat gattacaata aaggcaaatc agatgaagat aaaatcattt
    26341 atactgattt agctggaacg atgactgaac tgactggcgg attgatggat gctatcactt
    26401 atgtccttat tgcttttgca gggatctctt tagtaacaag tatgatcatg atcagtatca
    26461 ttacttatac atctgtgatt gaacggacaa aagaaatcgg tgtactaaaa gctttaggtg
    26521 cacgtaaaaa agacatcact cgcgtatttg acgcagaaac gtgtatttta ggtatttctt
    26581 ctgggatatt aggtgtgttt attgcatggc tagctacgtt ccctatcaat agcatcttat
    26641 acaacatgac ggatctaaaa aatgttgctc aactaaatcc tgtccatgcg attattctag
    26701 tgatcgtctc tactatattg acgatgcttg gcggtcatct tcctgcacga atggcagcga
    26761 aaaaagacgc cgctatcgct ttgcgtgcag aataaggttg tgacaaccac atttagattt
    26821 gagtcataag agcgctgaaa tagtagaaaa cagcaagaaa cgaggaagta atatgttcct
    26881 tgtttcgcgc tgtttttttt aattattttt tataattctg gtatactttt ctttcctttg
    26941 taccacaact cttctggcgt atacctgtct tttccattta tgaatactgt tccatctgct
    27001 tcaatcgtta cttcagtccc aatccacata ttacgagaaa gtgcgagaag attttttctt
    27061 tttcctcttc ctttttttcc aaattgcaag gcatcggtta tctcttctgt ccaatgagat
    27121 ttcacgtatt ttacttctcc gttttcaaat gtaactttca aaatattcgc tatattttcc
    27181 acttttactg ctttaggaaa tgactccttc atttttttca ctttccttcc tccaacacta
    27241 agtatgcgct ttcttttagt gataagtata tcatagttta ttctattatc aaaaaaacgc
    27301 atccattcca ttgtagaata ctttaccgaa caagttgaat tgttttcctc tttttcacct
    27361 tttactatag ataggtaaag acaactaagg aggaatctct catgacagat caaccgtatg
    27421 atttaagaaa agtcatcgaa gaactaaaaa cacttccagg tcaatatcac gaaacagata
    27481 ttgaaatcga tcctgaagca gaattatctg gtgtttaccg ttatatcggt gctggcggta
    27541 cagtaaaacg tccgactcaa gaaggaccag ctatgacttt caacaatatc aaaggtttcc
    27601 ccggcacacg tgtgaacatc ggaacaatgg ctagtcgtaa acgtgtaggt gaaattttgc
    27661 accatgatta tcgaacactt ggtcgtttct tgaaagacgc tgtagaaaat ccaatcaaac
    27721 ctgtgaaagt agacaaagca gacgctccag cacaagaagt cgttcatcta gctaccgatg
    27781 cagacttcga tattcgcaac attttgcctg cacctacaaa tactcctgaa gatgctggtc
    27841 catacatcac aatgggtgta gtcttaggat cagatccaga aaaaacaatg agcgatgtaa
    27901 cgatccatcg tatggttcta gaagataaag atacgatcgg tatgtacatc atgcctggcg
    27961 gacgccacat tggtcacttc caaaaacaat tcgaagcctt gaataaacca atgcctatca
    28021 ctatcaatat tggtttagat ccagcaattg ccattggcac gactttcgaa ccacctacca
    28081 ctccacttgg ctacaatgaa ctatggtgtg caggagcttt gcgtaacgaa cctattcaaa
    28141 tcgttgatgg tatagcagtc gatgaagtgg gtattgctcg tgctgaattc atcattgaag
    28201 cagaaatcat gccaaatgaa acaatgcaag aagatatcaa cacgaactca ggaaaagcaa
    28261 tgccagaatt tcctggctat actggggatg ctaatccagc tgtacaagta gtgaaagtca
    28321 aagcagttac tcaccgaaaa gacaatccaa tcatgcaaac aacgattggt cccagtgaag
    28381 aacacgtatc tatggccggt attcctacag aagcaagtat tcttgccttg gtcgataaag
    28441 ctattccagg aaaagtagta aatgtctaca atcctcctgc aggtggtggt aaattgatga
    28501 ccatcatgca aatccgtaaa gaaaacgaag cggatgaagg tattcagcgt caagcagcta
    28561 ttcttgcttt atcagccttc aaagaattga agacagtcat tttagtcgat gaagatgttg
    28621 atattttcga catgaacgat gtagtttgga ctttcaacac tcgtttccaa ggagataaag
    28681 atatcatcgt gatcccagga atgaggaacc atccattgga cccatcagaa ttaccagaat
    28741 ataatcctgg ctctattcgt ttccgtggga tgagtgcaaa aactatcttt gacggtacag
    28801 ttcctttcga tatgaaagaa cattttgtac gtgcaaaatt ccaagaagta gaagattggg
    28861 aaaaatattt gaaataatga atgatctgca actgttttga catcataaaa aggagtgcgg
    28921 aaaatggaac aattggcaca acggaagaaa aaaatcgtga ttggtgtaag tggcgcttca
    28981 ggcaccattt atgccatcaa cctagtgaag aaactaaaag aatatccgat gatagaaaca
    29041 cacggaatca tcagtccatg ggcaaaacag aacttaaagc tggaatccga cttatcacta
    29101 actgaattcg aaagcctgtt tgattaccat tattcaaaca aagatttagg tgctgctgtc
    29161 gccagcggat catttttgac cgatggaatg atcatcgtac cggcaagtat gaaaacagtc
    29221 gcaggtatct ctgttggttt aggcgacaac ctgattgcac gtgctgctga tgtaacaatg
    29281 aaggagcaac gaaagttagt cattgttcct cgtgaaacgc cattaaatgc cattcacttg
    29341 gaaaacttga ctaaattagc acgactaggt gtgcaaatca taccaccaat tcctgctttt
    29401 tataatcagc ctcaaacgat ccaagatctt gttaatcacc atacaatgaa acttttggat
    29461 caatttggaa ttgtaaatga gcattcaaaa cgttggcagg gtgattaata tggaaaaaga
    29521 aacgatagtt gaagtaacca aactaggttt cacgatccaa gctgctgtta aagtaatcgg
    29581 taaagactta ttgatcactt taacaggcgg agatacacca catatcggaa cagtcactac
    29641 attttctcgt gatacagaga tgcaaactgt acgtttccct agccacgatg gtcggttcca
    29701 taaagacgat gtcttagccg aaaaaatagc tgtgatcatc aaaactgccg ttccgggaag
    29761 ttgtacgatc acttccggta tccatgttga ccatatctcc aatgcacaaa tcaacgcctc
    29821 ttttccgatg tcagaagagt taggttacaa gattttagat tggttggaaa aaatagattt
    29881 ccatatcaaa gaaccagttt attataaaga tggtgaaaaa acattatgaa agaagaggtt
    29941 gtgaaatcag cgttatgacc tgagtatgag agaaaaaaat ggaaaaatag cttctcatat
    30001 tttgccgttt ttgagctaat accgagggac agctgattga acgccgttta tcaagaggtt
    30061 gtgataaagc cgcttcgttt cgagtagtaa gaaaaatttt gtaaacatgg gtttccacat
    30121 ttttgcataa atttgactta ctaccgagaa actggctttt gaacaccgtt tatcaagagg
    30181 ttgtgacacg tcttttgtca caacctcttt tatttttgct tcactgtaac tgctacaata
    30241 tcaccgattt cttttccgat gtttttacga atatcttttc gtagtccaag gatgtagcag
    30301 actgaaccat ctggattttt cacgcccata ttcacgatac tgccttcata cggcatattg
    30361 tcaaaagttg cttggacttt aactcttcca gcaccgaatt cttttcttat atcatacgga
    30421 aaaataatat atgccccgcc cttgtcgact tcggaagcat agatttttga ttcaaaagaa
    30481 taaatttccc tcattttatt tctcctattt tttacatcta gtatagcata gcaaaaaaaa
    30541 gaaggaactg tgacaaaagt cttgtcgcag ttcctttttt cgaataaacg gtgttctcct
    30601 gccaactcct cggtcacaag tcacaatagt caacaaacat gagaagctgt tttttgaaat
    30661 tgctccttgt gactcagagt taaacggcag gctcacaacc tcttccttaa acggtgttca
    30721 aaagcttgca tctacggcac taagcccaat cagatgaaaa atatggagtg ctatttttat
    30781 ctgattgttc ttactgcttg atgcagagca gctttttcac aacctcctac atggtgttca
    30841 aaatattatt cgccttttgc ttctttcact aattttgcga atgcttgttg ttggaattca
    30901 gcagctaagg ctttcgtacg ttgttcttgc tctgctttgc tcatttttgg attttcagtc
    30961 attgctttta ccataccgtc ttgaacataa gcttttgctt cactttccat cgtactttgc
    31021 tgtttcttca ctaattcagc taacttggct gcttggtccg tacttaattc tacccatgtc
    31081 tctgggattt tgaatgtatt cactcggctg tcatattcgt gattgttatc agcaatattg
    31141 aaccagaatt tatacatgtc gtttttgtat tcccaataag tagttttcgt cactttttca
    31201 gcatcgccgt ctgtcttttc tactttattt gtttcgttat ccgtaccagt tgcttttggt
    31261 ttttcttgtt tttcatttgt tcggtaaaga tacacttttt cttttccatt ccctaaagct
    31321 ttatacaaaa gcatatctgc tccttgggaa tcagcactgg aaacaatttt ttctgttttt
    31381 gtttccgtta ctttttccat tccaaagtga ttggacaaat tagcgacgat caaacttagt
    31441 gaagcgacga agattagtcc gaaaatcaaa gataaaaagg tttgccatgt ttttttagcg
    31501 aacacgttcg ttaaagcaaa agcaagcaag ctgacaatca aaactccaat aatcatgcat
    31561 tatcccccgc tttcattaga acaggttctt ttttcttcaa taaaaaggca ataataaagc
    31621 caagaatccc aaagatgatc gctacgataa acgcggcatg gtaacctgac agtgtggcat
    31681 tgattgcctg atctttataa gctaatggcg tattatccaa tacagatttg cttgggatat
    31741 ttgcttttgt cacattcgtc aacacactga tcaaaatagc tgtaccaatc gagcttgcga
    31801 tttgacggaa tgtattattt accgctgtcc catgactgat caagttattt ggcaatgcat
    31861 tcataccaga agtcgttacc ggcatcatga ccatggagat cccaaacata cggatcgcat
    31921 aaaggatcac gatatataaa actggagtcg tttttgtcaa gaaagcaaat ggtgcagttg
    31981 ccgctgtcaa aatgaacatc cctgtgatgg ctaaacgttt cgcgccgtat ttatcgaaaa
    32041 tcgcacccgt cacaggcatc atagccccca tgatcaatgc acccggcaac aacatcaagc
    32101 cagaatggaa tgctgattct ccacgaatat tttgaatata caatggtacg accatttctg
    32161 cccctaccat cgccatattt gttacaccag ctaaaacagc tgcaatcgtg aatgtagatg
    32221 atttgaatac acgaagttcc aagaatggat gttccaaatg taattgacgc catgagaaca
    32281 aggcgatgaa aatcacacca acaactaaga aaccaattac tttcaagctt ccccagccgt
    32341 catctcctac actagaaaat ccgtaaagta aactaccgaa tccaatcgtt gataaaatag
    32401 cggataaaat atcaatgcta ggatttgaca atggaataac acttttcatt aggaagaatg
    32461 ctaaaaccaa gacaagaaca acgattggca gtaccatacc aaacaagtca cgccaatggt
    32521 agttatcaat gatataacca gataatgtag ggcctaaagc aggagcaagt ccaataacga
    32581 tccctgacat ccccattgct gatcctcttt tttctggcgg gaaaatagat agcatgatat
    32641 tttgcaataa tggcatcgat actcccacgc cgcaagcttg gatcaagcga ccgattagta
    32701 atgtactaaa attaggtgct gtaaagcaag taatcgttcc gatcaaaaag atcgtcatgg
    32761 cagaaagata taaatttctt gaactgaatt tattgatcag ccatgctgtt attgggatca
    32821 tgatcccgtt gactagcaag aaaccagtcg ttaaccattg cacatcagac gctgaaatat
    32881 cgaaagcttt catcaatgta ggaaaagctg tcgtcaataa ggtctgattc aaaacggtac
    32941 aaaatgtacc aattaataag acaacaacta aaagtgtccg attatatggt tttccgtaaa
    33001 tatctacggg ttggttgtta ctcatatttc tccttctttc tgaatttttt ctgaaactac
    33061 tagaatactt tagtcctaaa ttcgctattt gtcaacctac ttgacacata agttaatacg
    33121 gatataatta agggaagcaa gaggacaaaa ggagtgacaa aaatggttgg ggcttccctt
    33181 aaacgtttgt tggaaaatga tcaattggtt gttggcatca gtgaattaag tgatatcgta
    33241 ggtgtgtctc cacgtcaatt aaggtattgg gaacaaaagg gattcattcg ttcgatcgaa
    33301 gctgatgcga attgtcctag aaaatatcgt ttacctacag tcataaaagt agaactgatc
    33361 aaaaaatatt tagatgacgg atttactttg tcaaaagctg cagaaaaggc agaaatccgc
    33421 caaaagaaaa tgcaccatgt ccgtaaagtt ttttcaaaag caatcaaaga tatcgaattg
    33481 atcgacgaac gctatacgat tttacaaatc ggtaacttca aagaagagaa tgctaagatg
    33541 tacatcattc atgacaacga gacagacgaa ttaacttaca agctccttcc tgtcgatgaa
    33601 aaagctgact acgaacagtt gttaaaagaa ctctaaacgc cactaatctc ctaagtacca
    33661 agctttttaa taagtagatg ctacataaat acttgaaaaa aggcctgttt gaacataaga
    33721 atatccgaac ggtgatctgc gtttctttca tatagaaagg aattctccat cactgttcgg
    33781 atattttttt atttttttcg gttactttct tctaatacat ctgttagcca atttgcatat
    33841 tctgtttccc gattgatcgc atgcgctaaa atcaggtagt gtccgtaatt ttcatgtttt
    33901 tctttttcag taggaaagat cgtttttttt cgctctaata gatgaagtcg tttttcttca
    33961 tgaaggtcac gctgctgctg aataatttct actaatgccg gatcattcac gtctttgacg
    34021 aaatacaatt tcagaacaaa ttcgtctcga ttaaccggta gttcattggt tggtgtatga
    34081 atccactctg ccaattgttc cttcccttct tctgttaatt cataaacttt cttttccaat
    34141 ttttcacctg tgatctctac ttgatgctga atgattcctt gctcttccat ttttgcaagt
    34201 tcaggataaa tctgactatg ttttgcttgc caaaattctc ctatttcatt tttgaacgat
    34261 tgattcaatt catatcctgt catcttcttc ttacttaata accctaacaa aatatatcga
    34321 agtgtatttt ttcttgccat cagttttccc tccttgacaa atctttaaat ccattctatc
    34381 ataaatgagt aatcgaaaac atgtagttta ttacatgttt taatttataa ggagatgctt
    34441 aaacatgaaa gaatttaaag aattggaaga ttttattgga actcacttta tctatacgta
    34501 tgacaatggt tgggaatatg aatggtatgc caaaaatgac catactgttg attaccgtat
    34561 ccatggcgga atggtcaaag gccgttgggt aaaagaccaa gaagctgata tcgttaaatt
    34621 gacggacggc gtattcaaaa tcacttggac agagccaaca gggacagacg tggcattgga
    34681 ctttatgcca aatgaaaaga aacttcacgg aatgatcttt ttccctaaat gggttgaaga
    34741 acatccagaa atcactgtta ctttccaaaa cgaacatatc gcagaaatgg aagcagctcg
    34801 tgaaaaatat gaaacttatc caaaattact tgttcctgaa tttgctacga tcacttatat
    34861 gggtgatgca ggtatagaca atgaagatgt tattagcgaa gaaccatacg atggaatgcc
    34921 agatgatatc cgtagcggaa aatacttcga tgaaaattat aaacgagtaa aataaggttg
    34981 tgaaagagag gttgtgaaat cagcgttctg acctgagtat tagagaaaga aacggaaaaa
    35041 tagttcctca tgttttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg
    35101 tttatcaaga ggttgtgaaa tcagcgttat gctttgagta ataagaacgc tagttagaaa
    35161 atagcttttc atattttcta ggctagtggg cttattaccg agaagcagcc gtttgaacac
    35221 cgtttatcaa gaggttgtga aatcagcgtt ttgacctgag tattagagaa acaaatggaa
    35281 aaatagttcc tcatattttg ccgttttttg agctaatacc gagggtcagc tgattgaaca
    35341 ccgtttatca aagattgtaa caactgcata ttcacacagt taaattagtg acttcaaaat
    35401 gaaataatgc tcccagcaaa aaattaggct gggagcatta tttcgttatt ctgttcatca
    35461 aaaatcagcg ttttgattcc atttcatcac aagttcctcc tcatttactg cttcatcttt
    35521 ttgctgtgtt tgaattgtaa aaccttgtga aagatagaaa tgaatagctg attgattttt
    35581 ttgatagact gtcaaagtca gtgttttatt ggtttctttt atttttttca ttagttgcgt
    35641 accgattctt tgttgccggt attccctttt cacaaagatt cctgcgataa actgatcaat
    35701 caccccagca aaaccaataa tttcttgatc tatttctgct aagtaaatca ctgcatctgg
    35761 taaagctttc ctaacagatt ctacttgatt ctcccaataa gttgatggaa tgaattcatg
    35821 agaatccaag ttagcatgca gccaaatcga tagaacagat tccagttctt tggcagacaa
    35881 ttttgttgtt gtgcggatca ttttttctct ccttacttat tcattgattt taaaataaaa
    35941 accatttttt tgaagctctt ttacaagttt tcgcccctca aaaaagtata tcttctcttg
    36001 ttttactttt ctaataccaa aagaaacagc aactttagga tcatcagctg aaacgattcg
    36061 ataagttcca ccaggcaatt gatccagctt gactttccca tgatggtcgc tttgtactga
    36121 aaacggcaca ccatttctag taagtgtttt tttcttatgt ttcaaaatga atgaactacc
    36181 atttaccggt ttccctgacg aatcactaat aataaatgaa aaatcaagca cttttttact
    36241 taatctatac tgatagatta gtcgtccgat aaacactaac agtatgacta cagctattat
    36301 cgttcctgca agaataaacc attgccgcat ggttctttcc tgatttcctt ttccttcttc
    36361 tttttttacc gattcgttat aaggaatccg atgtcctgtc actagcaaac gatgcgtgtt
    36421 gatcatatac ggcgtacatg taagcaaggt aactaagtct tctccttctg aaatcgttag
    36481 aacagacgtg tcatctggca gtaccacttg tatattttta acttgataag ccaatttctc
    36541 ccccaaaaca gtcaaaataa acgtgtctcc gttttctaaa cgatctaatt cggtaaataa
    36601 ccgtcgatta ggtaaaccgc tgtgagcaga gatcactgaa tgcgtcgaat ttccaccaag
    36661 cgggaaagag gttccttgca agacgcctgc tccattttca aggatcctac cattgagccg
    36721 atcataaagt gaaatattta attggatctt aggtatattg atacttccga tcagccattc
    36781 ctctaattgg ctggatgttg tactttcatt tctgttcttt tcattgaaag gatcagtacc
    36841 aggattgaat ccttggtcag ttaatcgttg ttttttctcc tccattttct ttcgctgcac
    36901 ttcaatttca gctgcggtcc gtttttgtac ttgttcgata cgtttttgat caataaaact
    36961 atttaaagaa tccacataaa atggatataa agcgatcagt aaccctacga cgatacatat
    37021 taataaaaac aaacgaaaca aaagtcgacg agactgtttt tgcttactct gcttcacatg
    37081 tgtcctccct tgcaagtttt tttgttcttt cttttattat atgggttcag gaaaaaaata
    37141 gaaaatgaaa gccgatgagg ttgtgaaaaa gctgtcctgc atcaagtaat aagtacagcc
    37201 caaaaaaata gctactcata ttttttgttg ggttgggctt attaccgcag atgcaagctt
    37261 ttgaacaccg tttaaggaag aggttgtgaa aactgcgtta tgctttgagt aataagagcg
    37321 ctagtcagaa aacagcttct catattttct aggctagtga gcttattacc gagaagcagc
    37381 tgattgaaca ccgtttaccg agaggtcatg aaaaagctac tttgtttcga gtattaagaa
    37441 aaattctgtt tcaaaacaag gagatgcaac aaaacttatg tcgcatcctc tctccttaga
    37501 actttcactt atgtttttct tgtaaaattc ataaatattt tcatgaattt tctaatttct
    37561 tgcctacctc tcgcatagga tatatgatga atttatctaa agtttgcaaa ggagttcgac
    37621 aattgaaaaa atatggccca tttttagcaa tttttttcat tgctttggca tttctcatgc
    37681 ctcatttatt cacacataaa ctaattttgg gtgctgatgc cccattccat tacaatcggt
    37741 tctatgatgc cgcgcaacaa attaaatatg gggatttcca ttattttcct tccctttatg
    37801 gattccagca aagtggcagg atcgtgaatg ccgtttacgg cccatttttc gcttatttac
    37861 aaggattgat cgtgttgctt gcaggtagct ggtttcatta tcagattctt tcaaatttca
    37921 ttctttatct tatttcagga ttttctatgc tgattttact gagatatgtc aatgttcgaa
    37981 attgggtaag tgtaccagta gccgttttat tcatgagtac gtattctatt caatactggt
    38041 ctatcaacca aggattcact agctggggga cttgttttta tcctttatgt atgatcccta
    38101 ttgctgattt cgtccttcat aaaaagttcc ctgttatcaa agtcgctgtc tcagtgggac
    38161 ttatgacaca gatccattta ctgtcttcga tcatgttagc tttgatgtat atcccttttt
    38221 atctttatta cttttttaac cagactgaaa aaggaaaagc actgcttcaa ctgattaagt
    38281 cggtcttgct ttattttgtt ctgacagcaa atatttgggc aggattagct attgtttaca
    38341 gtgggaataa gattcttcct ccctttgtta acagagaaat gagtcaaaag gcgatagatc
    38401 tggatggcag ttattggctc cattacccta aggcctttcc tatattgata ttatcaggtt
    38461 ttttgctttt tattttcttt caacaaaaga acaccttatt ccaaaaaatc gtgttgtaca
    38521 caagtcttgt tttctttttt ctttctacta gcttgattcc atggacgaca ttgatcgaaa
    38581 aaaatgtgcc ttttattagt ttgatccagt ttccatttcg atttttcgtg ccatttacgc
    38641 tattgcttct actttacctt gccctttcac tgaaccagtg gtctgaacaa aagtggttcc
    38701 gtcttttaag tatatttggc attcttattt tttcagcaca gacgatccag aacttatacc
    38761 agcatctgga aaagtgggaa aatgaaacat ttgtcagtcg acatacttat ctattcgata
    38821 cgccggaaga agcaaggaaa acattcttca gcaaagatat gtctaccagc ttgttcgttt
    38881 tccaaaaaac cacacctgac tatctgccaa tctataagga gacaaaagac aataaatacg
    38941 accggtataa cgaagaaata ctagaaaacg aaacaacttt tataaaaaca catccaggcg
    39001 gtcttttgac aatcaagtgg aaaaacactg gggacaaaac tgttcatatc cctgttatcg
    39061 tctacgatcg gactgtgcta cagcaaaatg gaaaaacatt gactgattat gaagtaacag
    39121 atatcgggac cccaatcgtg aagcagaaaa aaggaatcaa cgagctcacg cttcattatc
    39181 aaacgccaat ctatttctat ttcattcttt ctcttacttt gattggctgg tttaccttgc
    39241 tatgtttatt tatttatcat agatacaagc ttttacgtgc ttaggcaaaa gggataaata
    39301 catccgatca aaaaactctc caaacggtat aatcgtttgg agagttttat tttaatcagt
    39361 ttttttatgt tatatgttat attttttatt cgttcacatc taattcgatt tccaccccgt
    39421 agtgatcact gatgattgga ctatcgatcc catcaaaaat ccgttgatat tttttaacct
    39481 gcattccttc agggacgaaa ataaaatcta tacgcaattt ttctgtattt tgttcccaac
    39541 catctatttt cttttccaca gtagctgctt ttcctttttc attcgcgaca acatacgaat
    39601 cttgaagaag aaggcaactt tctcttacag tataataacc ttgagagtct actggattat
    39661 tgaaatcacc taggaaaaaa agtggtacgc gagtttccaa aaagtagttt tccaagtttt
    39721 tccactcaaa ataaaagcca ctttttttat ctgtccacca agaaaagtgg cagctcgaga
    39781 caaaaatgtc ctgatcatct atctttgttt tcccaatcaa gatcttgcgt gtctgataat
    39841 cttctttctt ttgagattgg gagacggtaa ggacctgact ttccaatgtt tttttcgtaa
    39901 gcagtgcatt tccttcttca tatttatcat aaccgatgtg gctcatttcc caactccaaa
    39961 aatactcttt tccgtgttcc tttaaaagct gaactaaacg gtaggcaaaa ttatctttac
    40021 ggataggtac ttgattcttt acctgacaga acttcattag cttcgcaggt tctacttctt
    40081 cagattccag taattggttg atttcttgta aagcaatcac ttcataatct tctaacagga
    40141 tcttatctgc taattcttgc aattttttct ctggttctgg ctcaagccag ctatgcgtgt
    40201 tgagtgtcat gaatttcatt ggttttctcc ttgtcttcat ttcgaaacaa aagggttgtg
    40261 acaaaatttt tgtcacaacc tctccagcaa atagtgttct cctgctaact cctcagtaat
    40321 aagctcaatg gaccaggagc tattttttgt ttggctgttt ttatcacttg gtgcagggca
    40381 gctttttcac gacctctcca ctaaacagta ttcaaactgc tgcttctcgg taataagccc
    40441 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttatgac ttgatgcagg
    40501 acagcttttt cacaacctct ccttacattt taatggagcc gatcacttta ccagctgtca
    40561 ctgtacctaa ttgcttgatc actacttctt ctactttatc gtttgtaaat acgatcatga
    40621 tcgttggatc tttttcttcg ttttggatca catcaagatc catctcagct aatttcgttc
    40681 ctgttcctac ttcttggcct tctttgacag caatctcaaa agctggctga ttcatttgga
    40741 ctgtatcgat tcccatatgg atcaaaactt cgataccatc tttcgtttcc agcccaattg
    40801 catgtttgct agggaaaata cttgtcactt ttccagcgat cggcgcaaca acttctcttg
    40861 atgttggaat aacagcaaag ccgtcaccca tcattttttg tgagaatacc ggatctttta
    40921 cttcagataa aggaatcact tctccagaag ctgcggaaac tagttcttgt tctataccta
    40981 agtattgctt actacctgct tgtccatttc ctgtagaaac catttctggc tctggaatct
    41041 ctgctcctga ttgcagcaga tcttcgatat ctgatttcaa gacatctgct tttggtccat
    41101 aaactgcttg gactccattg tctttgacga tcaatcccat cgcccctgct cgtttccagg
    41161 cttcttcagt ccctactttt tctcgatcat ttacacttac gcgtaaccga gtcatacaag
    41221 catcgacatc tttgatattt tcttttcctc ccagcaaatg aatgatctgg accacttgct
    41281 gatctgctgt tcctgttccg ctgttagata caacggtttc ttcactgtca ttgtcgtaat
    41341 ttccgttacg tcccggtgta gcaaagttga attttttgat caagaaattt gctaagaaat
    41401 acgtaacaac accaaaagcg atcacacaca acacgaagtt gataagatct ccacctagac
    41461 ccgctttgat agctaacggt gtccgagtca gcaattcgat attcccaaat gaatggaccc
    41521 gcaatggcaa gatatctgcc atcgcaaatg cagccccttg gatcactgaa taaatgacat
    41581 ataatggcac agcagcaaac ataaacatga actccaatgg ttctgttaca cctgttagaa
    41641 agacagccaa cgctgctgaa aaatacattg atttatatct tgctttctta tcaggatcga
    41701 cattacgata catcgcaaaa gccattccca tcaagatacc tgaagaacca atcatttgtc
    41761 ctactttgaa acgagccggc gtccagttat gcaacacaaa ctcatattgc gacatatccc
    41821 ctgcaccttt aagattcact aaatcagtgg cccaagccaa ccataacgga tcttgcccaa
    41881 atacttgtgt ccccgcctgt gcgcctgata aaatctcata cgatccgcca agctgtgtat
    41941 agttgatagg aatcgtcagc atatgatgca atccaaatgg caatagtaaa cgttctaatg
    42001 ttccaaataa aaacggtgct agaatcggtg cgctttcttg tgattctgca atccataaac
    42061 caaaattatt gattccagcc tgtacgtttg gccacaccac agcaagaatc aatgccacga
    42121 ttgttgacca taagataaca acaaacggaa caaagcgctt tccattgaaa aatgacaaag
    42181 catccggcaa ttttctataa ttatagtact tattataagc cattgccccg acaaatccag
    42241 cgatgatccc tacgaatact cccatattca aagctggtgc ttccaataca cttgtaaaaa
    42301 atcctttaac catgatcttc gtaccgaaca gagtgtgggt aaatgcatct tcattcgcaa
    42361 gcatgtcact tgtgacacca aagatagccc cagtgatccg attgattaaa ataaatgaga
    42421 tcccagctgc aaatgctccg cctgctcttt cttttgccca acttcctcct attgctaaag
    42481 caaataaaat atgaagatta ccaataattg cccaaccaat attttcaatt actccgcccg
    42541 ttgtgacaag cggcgtccaa tcaggattga tcaagggaat tgatttccct atactgatca
    42601 tcaacccagc tgccggcata actgctacca caaccattaa agcctttcca aatttttgcc
    42661 aaaactcaaa actaaatatc tttttcattc cgtttagctc ctttacgcaa tcggtttcgt
    42721 taaacgtatt atagcgtgcg ttttcttttt tgtaaatgct ttatttttta tttttctgtc
    42781 tataccattc tataaaacct ataatacgtg tgttcccttc tataattttc tcctaaaaat
    42841 ttattttttt aagctttttt ctatgaaata ttttcttttg tctattgact tgtgcaatcg
    42901 gttgcgttta ttatgaacgt ataaaacagc tcattaggag gagaaaaatg aaacagatca
    42961 aacgactatt tgctattgat ccatggaaaa tcagcacgac acaattagat aaaaaaaatc
    43021 ttcgactgca agaatcttta acaagtattg gaaacggtta tatggggatg cgcgggaact
    43081 ttgaagaaac ttactcaggg gaccaccatc aaggtactta tttggcgggt gtctggtatc
    43141 ccgataagac acgtgtcggc tggtggaaaa atggttatcc agaatacttt ggaaaagtga
    43201 tcaacgcctt gaactttatc gcgatagata tttcgatcaa cgatcagctg atcgacttgg
    43261 cttctttgga accagaagac ttttactggg aattagatat gaaaaatggt gtattatctc
    43321 gtacattcac aatcactact tctactaata aagtacgcat ttccttcgaa cgttttctga
    43381 gcattgtcaa aaaagaatca gcttatatct atttaactat agaaatgctt gaaggtagcg
    43441 gaacagtcaa actcgtatca aaattggatg gaaatgttca aaacgaagac agcaactatg
    43501 atgaacattt ttggaatgag attgatcgtg gaactataga agaaatcggc tatttgacga
    43561 ctaaaactat tccaaacaac tttgggattg aacaatttac tgttactgca gcaatgcgcc
    43621 atttcgttaa tggaaaatca gtcatccctg actatcacga agatacttta gcattacatg
    43681 gttgcttgtc tgcagaatta tctcaaggtg agacatggtc agtggaaaaa gaaattatcg
    43741 ttcttactag tagagatatt ccagaagcgc agcaagcaga aaaagctgtc tcagaattaa
    43801 cgaaccagac aaacaattat atccaagcaa aagaagagca aacagcggct tgggcagaac
    43861 gatggaagct tgccgatgtg gtcatcgaag gagacgaaga agcacaacaa ggtattcgat
    43921 tcaatttgtt ccaactattc tctacttact acggcgaaga tgaacgattg aatatcggcc
    43981 ccaaaggatt cacaggagaa aaatacggcg gagccactta ctgggataca gaagcgtatg
    44041 ctgtccctct ttatcttgct ttagccaaac ctgaagtaac taagaatctg ctgaaatatc
    44101 ggcataatca actgccgcaa gcaatccaca acgctcagca acaaggatta aaaggcgcgc
    44161 tttatcctat ggtcaccttc actggtgtcg aatgccataa cgagtgggaa atcacattcg
    44221 aggagattca ccgaaatggc gcgattgcct atgcaatcta caattatacg aattacaccg
    44281 gcgatacgga atatttgaaa aatgaaggac tggaagtttt agcagaaatc gcacgcttct
    44341 gggcagaccg tgtccatttt tcaaaacgag caaataaata catgatccac ggtgtgaccg
    44401 gcccaaacga atatgagaac aatatcaata acaattggta tacaaatacg atcgcagctt
    44461 gggtcttgcg ttacacgcga gaaaattatt taacttatca aaaagaagct gctatcacta
    44521 tcagcgaaga agaactagcc aaatggaaag atatcgcaga gaacatgtac ttcccagttg
    44581 acgaagaatt aggaatcttt gtccagcatg acacattctt agataaagat ctcatgcctg
    44641 tatccgatat tcccacaagc gatcttcctc tgaatcagca ttggtcttgg gacaaaatcc
    44701 ttagatcttg cttcatcaag caggcagatg tactacaggg gatttatttc ttcaatgata
    44761 cttttacaaa agaagaaaag caaaaaaact ttgatttcta tgaaccaatg actgtccatg
    44821 agtcttccct ttcgccaagt atccattcga ttttagcagc agaattagga atggaaagca
    44881 aagctgtaga aatgtaccag cgaacagctc gcttggattt ggataattac aataacgaca
    44941 cagaagacgg cttgcacatc acttccatga ctggtagctg gttgtcgatt gtccaaggat
    45001 ttgctcaaat gaaaactgcc catgatcagt tgagattcgc accatttctg ccaaaaactt
    45061 ggaaagctta ttctttccat atcaattatc gcggacgttt attgttcatc tcagtcacag
    45121 atgaacaagt tactttcgag ttattagaag gttttcctct agaaatttac gtctatgaac
    45181 agtcttattc tttagcggat cgtctagaaa taacgctaag aaaggaggaa gcaaatgttt
    45241 aaaggggtat tgtttgattt agacggtgtc attaccgata ctgctgaatt ccattatcgt
    45301 gcttggcgaa agcttggtca ggaaatcggc attacgatcg accacacatt caacgaacag
    45361 ctaaaaggcg tcagccgaga agactcactc tctttgcttt tagcttatgg cggaaaagaa
    45421 cactcttttt caaaggaaga atttgcagaa ttagcgaaaa gaaaaaacga ttattattta
    45481 gagatgatcc agacgatcga accaaaagat gtcttccccg gggtcgtgcc tttactggat
    45541 tctttaaaag aagaaaaaat caagattgct cttgcttctg caagtaaaaa cgggccattc
    45601 ctacttgaaa aaatgggtct tacaccttac tttgatgcta ttgccgaccc ggcagaagca
    45661 acaaacggaa aacctgcgcc agatatcttc ctccttgcag caaaagctgt cggactaaaa
    45721 gcagaagaat gtatcgggat cgaagacgcg caagccggta tccaagccat cctctcaagt
    45781 ggcgctcaac caattggcgt gggacgtgct gaagaccttg gagaggagat cccgcttgtc
    45841 cctgatacga cttttcttac gatcgattac ttgaaagaaa agtgtcatga ccatggataa
    45901 tccagagatc tccgtcgtac cagttccaga gactgaaata aagaaaatca cattgaagaa
    45961 cgactactta gaagtcgttc ttctcaatta cggtgcccgc ctgcaccaaa tcttcgcacc
    46021 agacaaagaa ggaaaatcag aaaatgtttt attgtcttac gatcgtttcg aagatgttct
    46081 aacagacaaa agcttttttg gagcaactgt cggacctgta gccggaagga tccgggatgc
    46141 ttcttggggt gagcatagat tggagaaaaa ctgcggatca caccatatcc acggtggcac
    46201 gaaaggctgg tctttccaat tttgggaagt agaagttttc aaaactccat catcaatagg
    46261 tgtggtcttt tacttaaaag atgaattttc tacctatcca ggaccaatca ctgctacgat
    46321 cacttaccgt ttaacaaaaa acgaattaga gatgacaacc tctgctagta gccttgttga
    46381 aaccatctgc aatccgacca accatgccta cttcaacctt tcaggaaatg gaacaagaga
    46441 catctatgaa catcagttaa cggtcttttt agatggtgtg cttgaattag atcaagaaaa
    46501 actgcctact ggaaattgga caaaaaaaga aaaactgcca atcgacttta gaaaatcccc
    46561 aacactgcaa gagatccttg catgttatcc agatggacta gatgatgtat ttcttttgca
    46621 tcatcctagg ctttccaaaa cttccttaca attattcgag aagcattctg gtcgccaaat
    46681 gactatagca acttctaata aaagtatggt attattttct acgactggat tcgaagctga
    46741 tttctctgtc aatggaaaac agatgcattc aaactacgga ttagcaatcg aaccgcaaga
    46801 aatccctgat atcgttcatt tccccaaatt tggatcgatc aacctccatc ctggtcaaga
    46861 acggatcagt cagacgattt atcggttctc tgcacaataa caagagaaag gtgtttttat
    46921 catggctatt accgtaaaag atgtggcgaa aaaagccggt gttgccacct cgaccgtctc
    46981 tcgtgtcatc aacgatcatc ctagtatttc cgagacaaca aagaagaaag tccgaaaaat
    47041 tatggatgaa ctaggttatg ttcctaacat gactgcccgc aatctaggaa aacgcatttc
    47101 cagtgccatc ggcgtaatct taccccctct tgattcaaaa gagcggatcg gtaacccatt
    47161 ttatcttgag atcatggaag caatcaacga agaagcgcgg aattatgata tgactacagc
    47221 aatcgctact gctaaaaatt ttgaattgct gttagagaac gtccagcgta tgcatttaca
    47281 aaaacaagta gatggtttta ttctagtgta ttcagatagt caagatcctg tcattgatta
    47341 tttataccaa aaccacatcc ctttcacttt gatcggacag ccttatcatc atgagaacga
    47401 aatcgtttat gtagataatg ataaccagct tctaggtaaa caagcaacag aatttttgat
    47461 ccagaatgga cataagaata tcctgtttgt gacaaacacg actcatgaaa gtttgtattt
    47521 cgaacgatat ttcggttatc aaaaagcaat gatgatggct ggtcttcctg cgttatccag
    47581 tgtagtacta gagcatccgg aggattatct gtcctttgag aaaacgttga aagaaaacga
    47641 agccacagca ttggtggtca ttgacgatat cttcgccctt cgcacgatgc agttggcgca
    47701 aatgtacggc tatgtcattc cagagacact ctccgtcatt agcttcaaca actcgatctt
    47761 ctcaacatta acacaccctt accttacaag tatcgatatc gatatctccg aattaggacg
    47821 tgtcgctatg caaaagctgc gcgaattgat ccatgaagaa ttgagcagtg gcgtacgttt
    47881 ggtcatccct catcgcttga tcaaaagaga gacagtcgtt cctttagaaa acccatacta
    47941 aatagctgac tcactaaata cgctgaccat ttatcctatg gtcagctttt tatgtaaaag
    48001 aagatactac ttctaacaca ttgtttatct gtaaaaaaga gcgtgggaca aaagtaaaga
    48061 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg
    48121 aaataagctg aaattcacaa aaatttaaaa agcaattttc ttgagtttct tcttatttct
    48181 tggagttaaa cgcttctgtc ccagcctctt ttttccttcc ttcaattttt tacacgtcga
    48241 cgattcaata aaaaaccgcc taatattgta aagacaggtg caagaatcga gaaaaagacg
    48301 aatggcaagt aatgcaacgc gctaactttc atcgttccca taatgaatgt gccgctgact
    48361 ccccacggta cgagagaatt gacggtcgca cctgaatccg ctagtgttcg tgtcaaatag
    48421 gtttttgata agccaacgtc atcaaaagaa cgcttgaatg tttctcctgg aagaatgatg
    48481 gacagatact gttctccaac gatcagattg attccgatac agctcaaggc agtaaacccg
    48541 actaaacgag aaggtcggtc catcttttct tttaattctt gaacgatggt atcaacgatc
    48601 agatatttga tcagtaaccc acctaatgcc aatgcaagta aaatcaaact agcagaacct
    48661 aacatactca tgatcccgcc tcgagaaagc agttcatcta gctttggatc aggtgtattt
    48721 gcgacataac cattcatcaa gatgtctgct gcttttccga ccgtcaaact tttctcatgg
    48781 ataaaagaaa gaacgagagc agctgtcgat cccgctagta atgtagggat agccggaatt
    48841 tttttgattg cccccccaaa taaaataact agtggaatca atgtccaagg agagatccaa
    48901 aagctcgctt tcatgttttg gatcatttca ttgatactag ctagatcggc tgaatgactg
    48961 ttatgtccta atatcgtata aaaaatcaac gaaaggacga atgctgggaa atccgtataa
    49021 cgcataccag cgatatgttc gaatagatta actcctccaa tacccgctgc caagttggtc
    49081 gtatctgata aaggcgagca gttattccct aaaaaggcac cggaaacgac tgcgcctgta
    49141 gtcattgcat cattgaatcc aagtaaatga ccgatcccca aaaaagcaat tccaatcgtc
    49201 gaaatcgttg taaatgaact gccgacagtc actccgacaa ttgcacaaac aagaaatgct
    49261 gtcggtaaaa agaaacgcac agaaacaagt ttaaatccat aaaccataat cgtagggatg
    49321 gttccagata aaatccatga agcaactaag acaccaatca aaagaaaaat caggattgga
    49381 atgatccctg gagtgattcc tttgatgatc ccgtcatgga tctcatccca tgtgaacttt
    49441 ttaaatctgc cataaaacaa caaacacatg aaagctgcaa ggatcgggat atgtggcgag
    49501 agctggaatc cgatgatcag tactcctagg agtgccaaca acacgatcaa tacagcgata
    49561 ctttctttta aagaaatatt tttattcatc gtcgatctcc tatttcaaat gaaaaggccc
    49621 tgccatgaaa aaactattca cttggcagga cctcctacta aatcagcgac aaaacgtcgg
    49681 ctgaaagact tatttttttt atttttcttc taaacttttt tctacctcaa tcccccaaga
    49741 atctttcttg aagtgattga tgatcaatgg aataacgaac atgaccactg tgatagctgc
    49801 tactaataat gcatcactca atgtcgctgt acctaatgta gcaaatacga caacgatgat
    49861 agagaagatc agcatgcagc tgactaccca agctaatccg tttccgtttt taccaattcg
    49921 gtaaggacgt tcgaattctg gatgtttctt acgcatttta agcaatgaaa cagcaatcaa
    49981 aatgtacacg atacaataaa taacagttgt agcatttgta agtgtcaaga atacagcact
    50041 tacgtcatcc attacaccat aaagcaaagc aaataatgaa ataacgattg attgtgttag
    50101 aacgacattt ctagatacgc cgtatttgtt ttctttatgg aatccgaatt ttggtggaag
    50161 gaatccttca cgagctactt ggatgattgt ttttgatggt ccagtaaccc aagctgataa
    50221 ttgtaaaagc acaccaataa ataccatgaa gctgaagata tttccgatca cttctggtaa
    50281 gcctaggatt tgacagtcaa tcaagatagg ctgcgtgata tttgctaatt ccatttttcc
    50341 atctggtacg acatttgcta caagcatcgc attgatgaca ttcaataaaa ctaatccgat
    50401 caaagcgata aatacgcctt ttgtataatt ttttgttgcg tctttcaaac gaggcatgta
    50461 aacagaactc atttcgatcc caacaaaaat aaatgtgata ccagctaagt atttcaatga
    50521 atcaatatgt tccaagtcag ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt
    50581 tgagactagt ccgactttga aggctgccaa aacacctaaa acgaacatga tgactactgg
    50641 gatatatacc cctagccata caccgatgtt cccgccgact tttgccatat cgaatttcaa
    50701 gttcaagatc gtgatgatcc agtagataac taaaatacaa cctaaaacga accagtgatt
    50761 attgccaagt tctacattac caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac
    50821 cattaccata cctgggaaca tttgcaccca taatagccat gcaacgacaa atccccattt
    50881 gctgcccagt gctgttttta cccataactg aggaccgcct tcttgaggaa gcatcgtgga
    50941 aagctcaccg gaaaccattg atatcggacc ggcaaaaaac aaaactgcaa aaattgaata
    51001 aaaaatcaat gtccaaccga ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag
    51061 tgccattgtc agaccgataa aggtcccaag acccatttta cttacttcag atttactcac
    51121 cgttgacact ctccttgatt ttttggataa aataacggtg tctttgaagt agtttcagca
    51181 ataaacgaaa caattcagcg aatatttgag gggattcttt gagtctattg cttgaagggt
    51241 ttcttcaaag acaacctcaa aacaaaggga tcgaagagct taggtctccc cttgctcttc
    51301 agatccaaac atgcgtcaga aacagttagt attcaagctg ttttctaaca tgattacctt
    51361 gcttattttg cttcgcttgc caataattgg tctgcgtaga ttttttccaa tttttcttga
    51421 agagctgctt tgattttttc agcatattct tcaaagtgtt cttctttgtt catgtatggt
    51481 gtcattacgc tggcacgcaa tacagttact tttccggcac ggttccattc ttcatctgag
    51541 aatcctagtt ggttcacaaa ttgtaatggg ctgttgccat agtctgggat agcaaagtca
    51601 gtatgtgaag ttaagaactc attaccgtaa atgcttcctt tcacgtatga agaataatca
    51661 tatacgtcat ggttcaattt attcattgca actaagtcat cgttgccttt ttctttgaat
    51721 acatagtcta ccatattgaa atctggataa gttaatggat gaacttcgat ttctttgtct
    51781 cctactttaa atgacaagtc attcaagaag ttgtagaaac gatgtgcacc ttcgattgat
    51841 gcacccatta atttaccata tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa
    51901 acgctggctg ctgttgcccc tgcttttgaa ccttccaaga tataagcccc aagtaatgct
    51961 gggatatctg cgcctttttc aaatacatat gtcgcaaagt aagagataac gtctctcatg
    52021 cggatatctt tgatgacgat accaccggca gaatatggaa catagcccat tttatgtggg
    52081 tcaatcgtta cagattctgc ttcttcgatt gctttatatg cactgtgtac ttcttctaag
    52141 atataatctt tgttttctgt aaagacattg tatttgtaat gaacatcttt taaatcttca
    52201 aatggaatga agttgttgtc ttcgtctaag aaaatcgcac ggccgtatcc gccataagca
    52261 gcatctacgt gtaagtagaa gtaaataccg tctttttcta atacgcgacg caatgcaacg
    52321 attttatcga taccgtcgat tgctccttct tctgttgatc ctacaacacc gactacacct
    52381 aggattggtg ttttttcagc tgctagacca cgaacgattt tttcaagttc gttgatgtcc
    52441 atgcgatagt tgtggtcaac tggcactgga atcacttggt ctaaaccgat accgatgatg
    52501 tcggcagctt ttagccatga atagtgttta gtttgtggaa ccaaccattt tcctaatttt
    52561 tctaaatgtt ttccgctacg tgctgagtga gctttgattt cgtcgatttt ttctgggacg
    52621 ctgtctaaca gattcatgat ttcttctgtt gacaagttca ttaattccca atcgcttttt
    52681 ccagcaacta gttcaggtgt cacttctttc attgcaagcg gcaatgattt gatgttgcgg
    52741 gcataccaaa gtccttctaa gtttgctaaa gaaccatcag caacgatgtg gccccagcca
    52801 tctttatagc tcattaattt tgcaaattcc attcctactt cttcttccat ttggcttgtt
    52861 gcaggagaag attcataggc aacgttatta ccattccata gcatagcaaa attgtacgct
    52921 agtaaagatg gcatcaatgt ttcagagttc atatgacccc agtaacgtcc ggcattatgc
    52981 caaggaacgg aatgggtacg catccgcgct gaaatttctg atagaacatc ctttgtttta
    53041 ttcacagtgt gttcaaaact agcactgctt ttttcttctg gcgtaataat aggcatgtct
    53101 tgaggcatgt agttttgacg ccagcctaaa tgttcatcca ccaattcatt caacaaagct
    53161 ttatagattt ggccgttctc agctttgtcc ccaatgaata gggcatttag gtttagatct
    53221 ttcgacaatg attcactcat agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc
    53281 cctacattac acctgttctt ctattctgta aactaagttt tggcctgatt tttaatcata
    53341 ttttaatatg tatcaattat ccgaattgtt cacacttttg aaataattaa aaaaaacgag
    53401 ggaatttaca aatgtaaact ccctcgcaaa gattgtatta acgacaacca gcgttctatt
    53461 ttaggatggg tttcaaaatt ggaatgttgt aatagtattt ccctttttca aggtaaaaac
    53521 ttactaaatt ctaatcaatt actttggcca aaaagtaatt tttcttacct tttcgaataa
    53581 ccacaaactt accatcgaat tcatcggatg gatttatgac aaaatttaaa tcggtaacgc
    53641 ggtcaccgtt aatacttata gctccgtttg aaacatcttc acgtgcttga cgtttagatg
    53701 gttcaatttt tgtggaaaca agcagttcca cgatattttc aggtgtgctt gaaatttcaa
    53761 cgttcggcat tttaccgaat ccttgcgcga tttcttccgc atttagttct ttaatatttc
    53821 cagagaacaa tgcttgcgtt attttttgcg cttctttcaa gtcttcttca ctatgcacaa
    53881 atcttgtaac ttcttctgcc aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta
    53941 cttttttcgc taagtcttcg atttcttctt gagacaagaa agtaaagaat ttcaagtatt
    54001 tcactacatc gcgatcatct tgattcaacc agaattggta gaactcaaat ggtgaagttt
    54061 tcttcggatc aagccaaacg gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct
    54121 tcagcatcaa tgggatcgtt aaaccgaacg cttctacttc tggtccttct aattttcgga
    54181 ttaggtctaa tccagctgtg atatttcccc attggtctgc tccaccgatt tgcaattgca
    54241 catcgtatgt tttatgcaga tgcaaaaagt cgatagattg cagaatttgg taagtaaact
    54301 ctgtaaatga gattcctact tccaaacggc tggcaacgat atcttttgct aacatcgtgt
    54361 tgatgttgaa gtttttaccg tagtctctca aaaattcaag taaagagatt tttgacaacc
    54421 agtcgtagtt gttcacaaat gtgatatttg catctttacc aaacaatttt ctcatttgat
    54481 tggaaagtgc gtccacgtta tgttgcactt gttccatcgt ttgcaacaca cgttctgttt
    54541 tgcggccgct tgggtcgccg atcgttcctg ttccgccgcc aatcagaata tatggatgat
    54601 ggcctgccaa ttggaatctt ttcatcatca taaatggaat caaatgtccg atatgcatcg
    54661 aatcaccagt tggatcaacg ccgcaataaa gtgaaatgct cttttcttca acgagctcgc
    54721 gaagtctttc ttcgtttgtt tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa
    54781 tattcatttt actgacctcc gtgtagtttt tttatgaaag caggaacctg ctagcatgtg
    54841 taattaatta tttcttcagg ataggatagg aaataaaaaa agtcccttgg catataagcc
    54901 aagggacgaa atctcgcggt accacccaat ttgtatgtct agatatccaa catacctctc
    54961 gaacatttaa cgctgtcgcg tggcaatcac tgccaaagac tcctgaaatg taattcgctt
    55021 taaagcatgt actggtttgc agcgaccacc agctttctga aacattggct ttaacaacta
    55081 cttcgtttcg atcttcgtct tgttattatt aatatgtgtc tatcatagag cgcaactttt
    55141 tctttgtcaa cagaaaaagg atattttttt taaattcctc attttttctt ttttgaatcc
    55201 gctctaattc cgaacatttt attttcaatt attgttaaaa gaaataactt tttctgtatt
    55261 ttttctgcaa tttatcgaga agtcacttta ctttgcctct tattctttct tcgttacaat
    55321 aaggaatggg acaagtaaaa aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca
    55381 aaaaattatt ttaggagcca cttctgcttt aactatcgtt attttaattc taagtttgtt
    55441 ttttgtcatc cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa aaataacaga
    55501 aaaagcaaaa gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc
    55561 tcaaagctac gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc
    55621 tactgaaaca caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg
    55681 gtctgatcct actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa
    55741 agccttccac tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt
    55801 caaccgttcg atcgaacaac tttatcaaaa tgattttgtt ttagttaatt taaatgattt
    55861 gatccaaaaa gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa
    55921 aaaaccattg gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg
    55981 ttttcctagc cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa
    56041 agataaagaa gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa
    56101 acatcctgat ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg
    56161 agttttaggt tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga
    56221 gattaaagaa gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc
    56281 ccatacatgg ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa
    56341 tgaattgtgg cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc
    56401 atttggtgca gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta
    56461 cttgaagagt caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg
    56521 acaattaggt acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca
    56581 atctgattta gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta
    56641 tgacaaagaa gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa
    56701 ctgacatttt atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc
    56761 cttgatcagg acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg
    56821 cttagaaatt ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt
    56881 caaatccact tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact
    56941 ttcttgcgta tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta
    57001 tgtcatcaaa tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc
    57061 actcatggca atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc
    57121 ttgccctaaa tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc
    57181 aaaatattta aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt
    57241 cactccagcg gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt
    57301 ctttttctct tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa
    57361 acttttataa tatatatagt tcaaatttat tctgaggttt attataacta tctattgaga
    57421 aagtatcaag ctagcaattc cttgtttatg aatt
//
LOCUS       DACDTH010000010        51289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDTH010000010 DACDTH010000000
VERSION     DACDTH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.010"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            320..1165
                     /locus_tag="IWK54_000688"
     CDS             320..1165
                     /locus_tag="IWK54_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9592835.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1446..2666
                     /locus_tag="IWK54_000689"
     CDS             1446..2666
                     /locus_tag="IWK54_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9592836.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2663..5383
                     /locus_tag="IWK54_000690"
     CDS             2663..5383
                     /locus_tag="IWK54_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9592837.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5367..6311
                     /locus_tag="IWK54_000691"
     CDS             5367..6311
                     /locus_tag="IWK54_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9592838.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6380..7390)
                     /locus_tag="IWK54_000692"
     CDS             complement(6380..7390)
                     /locus_tag="IWK54_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9592839.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7570..7914)
                     /locus_tag="IWK54_000693"
     CDS             complement(7570..7914)
                     /locus_tag="IWK54_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9592840.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7907..8341)
                     /locus_tag="IWK54_000694"
     CDS             complement(7907..8341)
                     /locus_tag="IWK54_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9592841.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8479..9216
                     /locus_tag="IWK54_000695"
     CDS             8479..9216
                     /locus_tag="IWK54_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9592842.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9209..10420
                     /locus_tag="IWK54_000696"
     CDS             9209..10420
                     /locus_tag="IWK54_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9592843.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10627..11406
                     /locus_tag="IWK54_000697"
     CDS             10627..11406
                     /locus_tag="IWK54_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9592844.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11476..12138
                     /gene="trmB"
                     /locus_tag="IWK54_000698"
     CDS             11476..12138
                     /gene="trmB"
                     /locus_tag="IWK54_000698"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9592845.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12236..13990)
                     /gene="ade"
                     /locus_tag="IWK54_000699"
     CDS             complement(12236..13990)
                     /gene="ade"
                     /locus_tag="IWK54_000699"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9592846.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14122..15051
                     /gene="cpaF"
                     /locus_tag="IWK54_000700"
     CDS             14122..15051
                     /gene="cpaF"
                     /locus_tag="IWK54_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9592847.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15008..16057
                     /locus_tag="IWK54_000701"
     CDS             15008..16057
                     /locus_tag="IWK54_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9592848.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16054..16332
                     /locus_tag="IWK54_000702"
     CDS             16054..16332
                     /locus_tag="IWK54_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9592849.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16307..16777
                     /locus_tag="IWK54_000703"
     CDS             16307..16777
                     /locus_tag="IWK54_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9592850.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16794..17096
                     /locus_tag="IWK54_000704"
     CDS             16794..17096
                     /locus_tag="IWK54_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592851.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17113..17484
                     /locus_tag="IWK54_000705"
     CDS             17113..17484
                     /locus_tag="IWK54_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9592852.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17456..17815
                     /locus_tag="IWK54_000706"
     CDS             17456..17815
                     /locus_tag="IWK54_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592853.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17987..18994
                     /locus_tag="IWK54_000707"
     CDS             17987..18994
                     /locus_tag="IWK54_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9592854.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19019..20203
                     /locus_tag="IWK54_000708"
     CDS             19019..20203
                     /locus_tag="IWK54_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9592855.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20640..22223)
                     /locus_tag="IWK54_000709"
     CDS             complement(20640..22223)
                     /locus_tag="IWK54_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9592856.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22801..22944)
                     /locus_tag="IWK54_000710"
     CDS             complement(22801..22944)
                     /locus_tag="IWK54_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9592857.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23166..23645)
                     /locus_tag="IWK54_000711"
     CDS             complement(23166..23645)
                     /locus_tag="IWK54_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9592858.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23718..24002)
                     /locus_tag="IWK54_000712"
     CDS             complement(23718..24002)
                     /locus_tag="IWK54_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592859.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24080..24274)
                     /gene="ssrS"
                     /locus_tag="IWK54_000713"
     ncRNA           complement(24080..24274)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWK54_000713"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24346..25632)
                     /locus_tag="IWK54_000714"
     CDS             complement(24346..25632)
                     /locus_tag="IWK54_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9592860.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25770..26441
                     /locus_tag="IWK54_000715"
     CDS             25770..26441
                     /locus_tag="IWK54_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9592861.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26407..26895
                     /locus_tag="IWK54_000716"
     CDS             26407..26895
                     /locus_tag="IWK54_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9592862.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26908..27855
                     /gene="prmA"
                     /locus_tag="IWK54_000717"
     CDS             26908..27855
                     /gene="prmA"
                     /locus_tag="IWK54_000717"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9592863.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27856..28620
                     /locus_tag="IWK54_000718"
     CDS             27856..28620
                     /locus_tag="IWK54_000718"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9592864.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28853..31066
                     /locus_tag="IWK54_000719"
     CDS             28853..31066
                     /locus_tag="IWK54_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9592865.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31077..31523
                     /locus_tag="IWK54_000720"
     CDS             31077..31523
                     /locus_tag="IWK54_000720"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9592866.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31694..32953
                     /locus_tag="IWK54_000721"
     CDS             31694..32953
                     /locus_tag="IWK54_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9592867.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33042..33989)
                     /locus_tag="IWK54_000722"
     CDS             complement(33042..33989)
                     /locus_tag="IWK54_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9592868.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34383..35675
                     /locus_tag="IWK54_000723"
     CDS             34383..35675
                     /locus_tag="IWK54_000723"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9592869.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35690..37459
                     /gene="aspS"
                     /locus_tag="IWK54_000724"
     CDS             35690..37459
                     /gene="aspS"
                     /locus_tag="IWK54_000724"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9592870.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37568..38442
                     /locus_tag="IWK54_000725"
                     /pseudo
     CDS             37568..38442
                     /locus_tag="IWK54_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
     gene            38708..40591
                     /locus_tag="IWK54_000726"
     CDS             38708..40591
                     /locus_tag="IWK54_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9592871.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40666..40950)
                     /locus_tag="IWK54_000727"
     CDS             complement(40666..40950)
                     /locus_tag="IWK54_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9592872.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40940..41674)
                     /locus_tag="IWK54_000728"
     CDS             complement(40940..41674)
                     /locus_tag="IWK54_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9592873.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41803..42426
                     /locus_tag="IWK54_000729"
     CDS             41803..42426
                     /locus_tag="IWK54_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9592874.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42618..43739
                     /locus_tag="IWK54_000730"
     CDS             42618..43739
                     /locus_tag="IWK54_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9592875.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43746..46874
                     /locus_tag="IWK54_000731"
     CDS             43746..46874
                     /locus_tag="IWK54_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9592876.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46893..50087
                     /locus_tag="IWK54_000732"
     CDS             46893..50087
                     /locus_tag="IWK54_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9592877.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50233..50940)
                     /locus_tag="IWK54_000733"
     CDS             complement(50233..50940)
                     /locus_tag="IWK54_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9592878.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16788 a         8948 c        10640 g        14913 t
ORIGIN      
        1 gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt gaactaatag
       61 agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat agttggttat
      121 tataaaatag taatatagct gatttggatt caattcatat ttatttataa atcaaaaata
      181 cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta cttacttaaa
      241 ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc agtgaattta
      301 taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt tagacggtac
      361 tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga aacatttacc
      421 ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta ataatattct
      481 caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg gatttgatgg
      541 agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa ttgattctgt
      601 cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat ctcagaatta
      661 taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta tcaaagagct
      721 aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata atattgagga
      781 aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc ctaatcgtca
      841 attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg ttactgaatc
      901 ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg tgtttgatcg
      961 tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag atagtttaaa
     1021 tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata gtcatcaaga
     1081 aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag tatctcaagc
     1141 atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata gaattctaaa
     1201 tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt caaacgaagc
     1261 gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt tttttcgaat
     1321 aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac gaacaaattt
     1381 tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa aggaggaaat
     1441 ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca tttgaaggac
     1501 tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg accgttttat
     1561 caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt gcaggagata
     1621 attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa cagatgaaac
     1681 ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat ttctatcaaa
     1741 aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc gaaacggtag
     1801 agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga tttagttatc
     1861 gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa ctcacagatt
     1921 accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac tatgctcctt
     1981 tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg catatccatg
     2041 tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct caaggtcata
     2101 ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa ggaagttgcc
     2161 aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata tcattgagga
     2221 aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca aaagtagagg
     2281 atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat acagatgttt
     2341 tggaacggat acaatcagga gagctattag actatttgtt agaggaattc tcagatagga
     2401 taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat acgaagaccc
     2461 cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag acacctcaag
     2521 atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga atattgagcg
     2581 aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt aatcaagatt
     2641 ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg cttttggaaa
     2701 atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg ggggaaacga
     2761 agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt tcccttcaaa
     2821 aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg gcaaaatatg
     2881 gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc aaaatcgcgg
     2941 aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag aaaaactgat
     3001 cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag agcagttgat
     3061 ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt taggcatcac
     3121 gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac aacaattatt
     3181 caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga aagagctgca
     3241 agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga tccaacaaga
     3301 acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg cgaacaaaca
     3361 attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat ggcaatctct
     3421 caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc tggagtcttt
     3481 ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa cactggaaaa
     3541 gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc aggagcagga
     3601 actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag aatggcaaag
     3661 attacaaatt gagactagag aaacaaattc agaattgaag attttggaac aaaggtggca
     3721 ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga agcatcttct
     3781 ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc tccaaatagc
     3841 taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga cctatccagc
     3901 attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt atatgctgat
     3961 ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga aagtgatcct
     4021 tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac caaaaattaa
     4081 aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag gacaacttga
     4141 tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg gagaagaaaa
     4201 acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag gaaagatgga
     4261 tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga aattatatgg
     4321 cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac gacaaatcga
     4381 ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa tttcagaaaa
     4441 aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg ccagaagcta
     4501 tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac aaaaagtatg
     4561 gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt cggaagtacc
     4621 tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca atgaattatc
     4681 ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat ctgtaagact
     4741 gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag caagtgaaaa
     4801 tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat tggacgaact
     4861 ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg aatggggagc
     4921 acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg aaagacaatt
     4981 accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag gaaattatca
     5041 gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag aatttcccat
     5101 ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat ttgcttacct
     5161 ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct ggcttcatta
     5221 tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag aaaagtatca
     5281 aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga tgaaccaaat
     5341 cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta atcgatgagc
     5401 agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa aacggaaaaa
     5461 aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa tactgggatg
     5521 cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta aacggtaaaa
     5581 gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta acaagggtag
     5641 atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa gaagaaatgg
     5701 aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat cgtattgttc
     5761 gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca aaaagaaacc
     5821 atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac ttagggaaat
     5881 caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt atcattttgc
     5941 atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat acgcttgcag
     6001 gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc tgtcttgcgt
     6061 tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt ttagcacacc
     6121 atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca gaaatattac
     6181 atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt cgtcaaacag
     6241 aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc tatgtaccaa
     6301 aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag acataagtct
     6361 tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt gaagattctt
     6421 ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt tcacttgagt
     6481 ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag gcatcatctt
     6541 tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg aatgcattgt
     6601 cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta tccatgtcat
     6661 tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca taagaagtag
     6721 gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa gctgcttctt
     6781 ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg ccatcttctt
     6841 ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct ccatcatccg
     6901 ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt tgtgcttcta
     6961 cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata tctacgtgag
     7021 ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa gtatctttcg
     7081 tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc gcttgtttgt
     7141 cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta ttgaatactt
     7201 tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt gcttcatcat
     7261 agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta ttagtatcgc
     7321 ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa ataattgatt
     7381 tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata ttaactatac
     7441 catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt caaaatttca
     7501 tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct gttgagaaaa
     7561 taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt tttctacttg
     7621 ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga tatctttttt
     7681 gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt cgatcaatgc
     7741 gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg cttcttccaa
     7801 ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata acagacctcc
     7861 gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa tcatcgcagg
     7921 acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca taactatctt
     7981 gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg actaaatgga
     8041 tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag atattaagac
     8101 ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca cgcgaaaaaa
     8161 cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt ttaggcacaa
     8221 ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc ttgtcgtctt
     8281 catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata cagttttcca
     8341 tataaaacat cctttctata aagaataaat acttcagctt aactttacca taagttaaag
     8401 ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata aataaaaata
     8461 aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg gtacggacat
     8521 ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt agggttgatt
     8581 ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct gctggaagct
     8641 tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga agaatatcgc
     8701 aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac attgagagaa
     8761 catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat gaaaagagca
     8821 gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc agctaatttt
     8881 tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga gccttcttta
     8941 tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc attgcttgaa
     9001 ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca tattttagct
     9061 actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat acgagcagac
     9121 ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt ggacgaaatc
     9181 tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac gtttagcaag
     9241 acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt ttgttttagt
     9301 ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga aaaccctgca
     9361 gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct gtttattcgt
     9421 gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc ctaaagaaaa
     9481 agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc cgttgatctt
     9541 tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg agcaatcctt
     9601 ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc atctgcaaat
     9661 caagagagca gaattgtttc gactagatca acaagttatt cgttattgga aattaggctg
     9721 gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat gggcaggaat
     9781 agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc aacaagaaaa
     9841 aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc agatctatca
     9901 atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa gaagacgcta
     9961 tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc tttacttgtt
    10021 tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat tagtaattat
    10081 aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta tcggtttctt
    10141 atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc gctatatggt
    10201 tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc agtatctact
    10261 cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag tcgtattaga
    10321 tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc tctttgttaa
    10381 actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct gtgtagggta
    10441 aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct attttttttg
    10501 agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt gcttgacgtg
    10561 tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag gacgggagaa
    10621 tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa aggcgacaca
    10681 ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg aaacacgact
    10741 ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg gacaaaacga
    10801 acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt gctgactcct
    10861 aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca tcattcaaat
    10921 tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga gaagatgctg
    10981 caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct gcaaattgtc
    11041 catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc agtagtacac
    11101 ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct agtcgattgg
    11161 gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg aaattatgtg
    11221 ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac tgacgaaatg
    11281 cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac cagatattgc
    11341 aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg aattttcagt
    11401 ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat tagaggaaag
    11461 gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat gttagcggct
    11521 cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa tgaattgttt
    11581 gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt catcactgga
    11641 atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt cagtgttgtt
    11701 tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact tctacatgtg
    11761 gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat ctatctgaat
    11821 ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata taagactttt
    11881 cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa aacagataat
    11941 caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat cctcaaacag
    12001 gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga gtatgaagaa
    12061 aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca agataagacc
    12121 aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat atccataaat
    12181 aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat ttgatttaaa
    12241 atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga gaaaatgaaa
    12301 ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag aaagaaagtg
    12361 tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt attaattcat
    12421 tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct ataggaagag
    12481 aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct ttttgaatca
    12541 aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact agattatgcg
    12601 agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct ttcactatgc
    12661 ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt aattggtctt
    12721 tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc aaatctttag
    12781 tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa agaagcagct
    12841 ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac gtatatgaat
    12901 ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact aatgttccct
    12961 tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag aaatcagctt
    13021 gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct gcattcaatg
    13081 ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga cggacgatat
    13141 gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg acaaagaagc
    13201 atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct tttgcaacgg
    13261 tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct tcttttgcag
    13321 ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac tcttccatgc
    13381 cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat tggatctttt
    13441 ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca gctaagccta
    13501 aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt gcaccattgg
    13561 tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc atgagtgttt
    13621 gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt cctatttcat
    13681 ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa aattcgcttg
    13741 gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc actagatatt
    13801 gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg atcattcctt
    13861 cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg atcttagcat
    13921 gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat ttccttaatg
    13981 ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata aattcggctt
    14041 ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa aagacgtatg
    14101 ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat gataagtgaa
    14161 agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt attttacatc
    14221 tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc agatggacag
    14281 caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa aagaaaagct
    14341 cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg gttgtcttct
    14401 gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg tacttttgaa
    14461 acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa aaccgtcaga
    14521 cgtggattat accttttcag cgggacagtc ggatcaggca agacttctct aatgtatcgg
    14581 ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga gatagaagaa
    14641 ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga tacacttttg
    14701 aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag agatgaatca
    14761 actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc aaccgtacat
    14821 gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa aaaagaagag
    14881 ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc caatcaccat
    14941 ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa atcatgggag
    15001 gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta aaggattaac
    15061 aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat ctggttttag
    15121 tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg ttattttggc
    15181 aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt cgcaaatagg
    15241 atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga atctgccgac
    15301 aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca atgatttgaa
    15361 aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct tgtttagtat
    15421 gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag agcattgggg
    15481 tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag cggttactct
    15541 attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc agagtgaatg
    15601 gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg cttatttgtc
    15661 actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca tagtctgctt
    15721 aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga ttcaaggatt
    15781 ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat cagaagaatt
    15841 cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac tgatttttta
    15901 cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc attttattca
    15961 gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg ctgttttatt
    16021 acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat atcttattat
    16081 agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt attagtgttg
    16141 ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag tcgtgaagcg
    16201 atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa tggaagagtt
    16261 cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc agaaatctat
    16321 cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat cactcttact
    16381 gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg catggaagaa
    16441 tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt ctacgcaaaa
    16501 aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag agaatcaaat
    16561 catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata ttcctgatga
    16621 gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga atgaaagttc
    16681 actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt atcaattcca
    16741 gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt ttgttggagt
    16801 cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt ggccagctgt
    16861 ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga atgatttatg
    16921 aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa gaaatgatcc
    16981 catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt gtcaatatgg
    17041 agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct gattgaatgt
    17101 ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt gattcagcaa
    17161 accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct aatattttta
    17221 gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa tcagcaactg
    17281 cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat gatacgtaaa
    17341 agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct tcaattattg
    17401 gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga tggatatgcc
    17461 atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat tttctgtctt
    17521 gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct accgtttgtc
    17581 aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga agcagatggc
    17641 tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact acacaactgg
    17701 atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag tctcaccgtt
    17761 tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag aatgatggaa
    17821 tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa aatgtttgcc
    17881 gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc gatagaatta
    17941 aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt taccagaaaa
    18001 aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga atgcattggg
    18061 aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg attatcaagt
    18121 acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag ctctttatga
    18181 agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc aattactttt
    18241 attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc ctgatagtat
    18301 cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag caccggttaa
    18361 gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt taaacctatc
    18421 gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat tgttggctgt
    18481 cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc aagacgggtt
    18541 gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa tcggttatta
    18601 tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc atagttatgc
    18661 acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt ttggtttgtt
    18721 tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa aatggtttaa
    18781 agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc gtaataaaca
    18841 atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac aagtaaaaga
    18901 agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg aatttttcaa
    18961 cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg agagtcatat
    19021 gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat tatatcaaat
    19081 gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac aagattctat
    19141 ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata tcgaaaacca
    19201 cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt taagctctta
    19261 tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt atggggattc
    19321 agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat ttgctccttt
    19381 gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac ctgacatttt
    19441 gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa attatttata
    19501 tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct tccatggcac
    19561 aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga tcgaagaact
    19621 aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg atggaggtaa
    19681 atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg gtacacgttc
    19741 aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga ttgatattga
    19801 tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag gattgtctag
    19861 tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag cttatgacat
    19921 tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga atggtgttga
    19981 tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta gtgaaatcat
    20041 caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat tgcgtggtga
    20101 agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc caacagatga
    20161 ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag cattcgttag
    20221 gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt gcggagaaga
    20281 ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt cttagggaat
    20341 tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg attttcagga
    20401 aaatcccaat attttatttt cattcatctt tataagggac attacatatg taaaaagaaa
    20461 aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta tgtttagcta
    20521 gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc cacctgtact
    20581 ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga aaaaacgtcc
    20641 tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat aacgagtgct
    20701 tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag cgttccttga
    20761 tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg attggttggt
    20821 tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc caaacggatt
    20881 ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata aaagccactt
    20941 ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc gtaagccagc
    21001 aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat ttgcaagcta
    21061 ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa tttgagtaag
    21121 gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg atagactgca
    21181 aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt ttgaatgatg
    21241 aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt tttcggggga
    21301 ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt ggcttttttg
    21361 aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat catgactttc
    21421 aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc caagcgagct
    21481 tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc accttcttcc
    21541 agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga aacgaccaga
    21601 accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt aagatctaaa
    21661 tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat tttggctaaa
    21721 gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg acagtgtgga
    21781 atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc gcctagttct
    21841 tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt tgtatctggt
    21901 tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat ctcagcggca
    21961 gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg ttcgaccgtc
    22021 ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc tctgccggtc
    22081 aagatttgta aaaaggttga tttgccagtt ccgttgatat cgactaaggc aattttttca
    22141 ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat tgttttttta
    22201 atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa aggccatggt
    22261 tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct tgagatggcc
    22321 attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca gcaactgtaa
    22381 aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta cttgttattt
    22441 tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc agtgtacctg
    22501 aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga tgtgtaattc
    22561 tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca tcattgtatt
    22621 ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac tttgtctaca
    22681 tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct ctttggaaat
    22741 ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg tagcctttca
    22801 tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc cgaacccgaa
    22861 ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat attttttcgt
    22921 atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat gcttaggtcg
    22981 ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat tttacttgaa
    23041 ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg cttgccgcgt
    23101 cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg caggtcgtct
    23161 acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc agttcggaca
    23221 acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc aataaataaa
    23281 cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg attatcttct
    23341 gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga ggttacatta
    23401 ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt tgccatttca
    23461 gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga acgtgtgtca
    23521 atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac cgccttttta
    23581 aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt gtattgttgt
    23641 aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta gaacacttta
    23701 gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa tcggatactg
    23761 aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa gcgttgattg
    23821 aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc gtaaaattgt
    23881 aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc ctgtttatta
    23941 aataaataat agctaacgaa taataatact aaagtggcga taatcattaa aatcctaatc
    24001 atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa gagcaaattg
    24061 ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg tagtattgat
    24121 cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa atgataaggc
    24181 atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt tcggtcaaca
    24241 cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca tatgaattta
    24301 aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt tttttggagg
    24361 atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt catatttccc
    24421 tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat attgctgatt
    24481 gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt tgagtgattt
    24541 cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt tgccctctcg
    24601 aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg gcgacaagca
    24661 aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca atttttcagc
    24721 agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac ctatatcttc
    24781 ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct caactaaacg
    24841 ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt gaaaagccga
    24901 aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc tttggacaca
    24961 ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag gagtcgatag
    25021 tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg cacgcgaaag
    25081 gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc gttctttatc
    25141 ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt tgacttcaaa
    25201 gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct cagtagtcgc
    25261 tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct gttttgtttt
    25321 atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt ttgcttcttc
    25381 cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc gaaatgcata
    25441 attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag gaggtccata
    25501 tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt ttccttcccc
    25561 gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc gataagccaa
    25621 tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta tatcatattt
    25681 agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt atccaactaa
    25741 tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata gatgaatttt
    25801 tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat ttggagcatg
    25861 acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac ctaggtccag
    25921 aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg gcaatgtatg
    25981 aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg aacatcatta
    26041 ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc gccgcaatga
    26101 ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc aacggtccgg
    26161 gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct atagcagata
    26221 gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat gctttgccta
    26281 gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt gttgtgtcgg
    26341 gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg ggttggagga
    26401 aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga aaaacaagtg
    26461 acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga gatcgttgtt
    26521 cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg gatcgtttcg
    26581 caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca gaaatctgtt
    26641 tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg gttggcaaaa
    26701 tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg gcaaagcgat
    26761 ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca ctgggataat
    26821 caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac ttatttaccg
    26881 tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt agaaactgaa
    26941 agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag tggtgtcgcg
    27001 attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga gattttagat
    27061 aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt tccggaaacg
    27121 actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt acctgagttt
    27181 ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag caactgggca
    27241 acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac ggttgcgcca
    27301 agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact agatcctggt
    27361 atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc actagaaaca
    27421 gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt gctaagtatt
    27481 gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga agtagccgtt
    27541 cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca tgtatcagct
    27601 aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa cattttagca
    27661 gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac aggcacgctg
    27721 atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat gttagaacaa
    27781 gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat tttgaaaaaa
    27841 ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc aaaagactat
    27901 tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat ggaacccgag
    27961 gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat cacggagatt
    28021 actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa ggaactgcca
    28081 agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg gatcgttcag
    28141 aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc ctctgtcgtt
    28201 aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat tgctcaagag
    28261 gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga aacgaaacaa
    28321 gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga ggaatcagca
    28381 aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc agatagtcga
    28441 atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga ttattttgta
    28501 gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga aacagcacct
    28561 ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc tttcagctaa
    28621 cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc aacaatttgt
    28681 ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa gcttttaaaa
    28741 agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat tatcaaacag
    28801 atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc ctgtgacaaa
    28861 agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca tgggtcccga
    28921 acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg acggacagtt
    28981 tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta tattagcaga
    29041 tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg ttgaagatac
    29101 agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta tgttagtgga
    29161 cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc tagcagaaaa
    29221 ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg tcaaattagc
    29281 tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc aaagaaggat
    29341 cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga tcagccggat
    29401 caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt attaccgtat
    29461 cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg gtactgttga
    29521 agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg gacggccaaa
    29581 acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca acgaaattta
    29641 tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg ctgtattagg
    29701 agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata ttgccatgcc
    29761 aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag gaaatccggt
    29821 agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg ttgctgcaca
    29881 ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga cgaaacaatt
    29941 aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt ctgaatttat
    30001 ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac caaaaggaga
    30061 cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta tccatacaga
    30121 tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc ttgattacaa
    30181 attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg gcccaagtcg
    30241 cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac gtttcttcaa
    30301 agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca aatgtattac
    30361 agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag aagcactcga
    30421 tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg gagaagttag
    30481 ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa aaatcgagca
    30541 gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg acaaaatgaa
    30601 agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa ttcgtatcag
    30661 tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta aaggcagagg
    30721 gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa atgtagcaga
    30781 acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt atgatgcaga
    30841 tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc aaacagtcaa
    30901 tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata aaatggcgac
    30961 gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg tggataaaat
    31021 caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg acagatatga
    31081 gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa gaaattggaa
    31141 aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg caaaaagatg
    31201 tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa caaggaaaaa
    31261 tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa ttcacactgt
    31321 ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga ccagaaacag
    31381 ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc acagtcgcta
    31441 ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct gtaacgatca
    31501 ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac tatgaaacac
    31561 gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg caaaaagcgc
    31621 ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta ttttttatag
    31681 gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag atttaaaaaa
    31741 attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg acgctcccgt
    31801 cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat cagcagcaaa
    31861 agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat tagtattgaa
    31921 gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc accatcggaa
    31981 aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc gtatcttaga
    32041 taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat ttgatttaag
    32101 taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat cagcgatttc
    32161 taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg gaacgtttac
    32221 ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa aatgggcgca
    32281 aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg tggcattata
    32341 tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag tggcagctgt
    32401 tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg cattgatggg
    32461 acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact tttcgacgtt
    32521 acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa tgctacctgc
    32581 gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca gttgggttat
    32641 tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag attcctttat
    32701 gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat atgagatgat
    32761 tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat ggatcactct
    32821 tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct ctgtacctga
    32881 acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc gagcgctact
    32941 ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac agtgctgatc
    33001 ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc acgaaatgct
    33061 tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg gacattgtca
    33121 gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg attcttcatg
    33181 taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat ggggcgaagt
    33241 acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg atttcttttt
    33301 cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc tgtcagcaaa
    33361 ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag ataagggaat
    33421 aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac atcgacatat
    33481 ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc gactgctcgg
    33541 atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt tggtgtgctt
    33601 tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg ttttagtgca
    33661 tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc tttatttggt
    33721 acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt ttccttcagt
    33781 acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt atcgatcaaa
    33841 ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc ttccaaaccg
    33901 tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc tgcgtagact
    33961 tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc tagtctaaaa
    34021 caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca gatatctctt
    34081 gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg aaagagcgag
    34141 taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct aaagtttcat
    34201 tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac gagcgttatt
    34261 cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat ttcattcacc
    34321 cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag ggagagaaaa
    34381 ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa tcagaaaaat
    34441 ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat aatgaaatgc
    34501 gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat acaacggata
    34561 tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg acgcttcgac
    34621 cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc ggcccagagt
    34681 atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa cgccctcaaa
    34741 aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca gaaaaccctg
    34801 caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg gggatcacgc
    34861 aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc tatcgtcaag
    34921 cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct aaacgccgcc
    34981 ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa aaatttgtgg
    35041 cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat tttgataccg
    35101 ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat atggttcgcg
    35161 gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca aaaatggggg
    35221 cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa ctaggtggtc
    35281 cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg actatggagg
    35341 cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc gcactcggag
    35401 acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt ggtttttcag
    35461 ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca gataaagcga
    35521 atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg attaatgtca
    35581 aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat gaacattttg
    35641 atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa tggcacaaag
    35701 aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta cattaaaagg
    35761 atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac gtgatcgtga
    35821 aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg aaatcgcgga
    35881 taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac gcggagaaaa
    35941 tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg aaatcgtgat
    36001 tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa tcagtgacga
    36061 aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta aaaacatcaa
    36121 attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg actttctcga
    36181 tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact acctagttcc
    36241 ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat tgtttaaaca
    36301 attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt tccgcgatga
    36361 agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa caacgttctt
    36421 aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa tgaaagaagt
    36481 tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag caatggctcg
    36541 ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc taagcgatac
    36601 agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg gtgtcgtgaa
    36661 agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg atcagttagg
    36721 tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag aagaagacgg
    36781 attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca ttaaagcaac
    36841 agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa tcgtcgctgc
    36901 tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg atgaatcgaa
    36961 attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag aagaaggtcg
    37021 atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc cgttattggc
    37081 tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg gttatgaatt
    37141 aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac tagaaactct
    37201 aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag ctttagatta
    37261 cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa tgttattagc
    37321 cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag cagcagatgt
    37381 gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga atattgatgt
    37441 aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat acgcttcttt
    37501 tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata taaagcgggg
    37561 cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga ccaagctttc
    37621 ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt atcagccagg
    37681 acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt tgtcaaaatc
    37741 actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata tacctttgtt
    37801 tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta tcacagtcac
    37861 tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg atccaatcat
    37921 ctgtgcgttg tttggggggc tgtgatggga ctaggtattg ggctagcgtt gagaaacggc
    37981 ctatcatcgg gcggtctaga tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg
    38041 attggttcta tttcgatcta cttcaatgca ttgatcgttt ttgttgccgg aattctattt
    38101 ggctggcaat atatgtttta cagtacgttg tctattttcg tcagtggaaa agtaactgat
    38161 gtcgtttata ctaaacaaaa gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa
    38221 gtagtaaatg aaatccaaaa ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa
    38281 ggtgcttata agcatgataa gcagacagta ttgatgacga tcgttacacg atacgaactg
    38341 cctctactta gagaagcaat gaaagcttct gatccacaag cttttgtaag tattgcagaa
    38401 aacgtccaga tacttggtcg tttctatgag gatggcattt agatcgagtt tgacaaaaat
    38461 tcatgaaata ctgctgtaga cagacgttcg tttgtctgca gtttttttct accatagtag
    38521 aatcagcaaa gaaaaactgc ttttttattt gaaattttct taaagttgaa aaaaagcatg
    38581 tttatttttt taaatttatg ggatgataga aaaaaatagt agagaaagga cacgattcta
    38641 cttgctattt tcacttaata cccgtatatt acgaatgaaa aaagtttatg aaaagttgga
    38701 atattgaatg gaaaaacaaa aaaggttaaa aaacagccgc tacatcactg ggtttgatgg
    38761 gctccgttca ttagctgtgg tgggtgttat cttctatcac cttctgccaa ctagtatgaa
    38821 aggcggctat ttgggcgttc cgattttttt cgtcgtatct ggttatttga tcacggatct
    38881 gctgcggcag gaatgggaac aaaatggaaa aatcaatatt tggcaatttt atgtcagaag
    38941 aatgaagaga ctctatccgg cacttgtttt tatgcttgtg acagcttcgg cttatatcac
    39001 tttgtttcag cgagggctgt taaacaatct tcgaggagtt gtggtcagca gcttactgta
    39061 tgtcaacaac tggtggcaaa tcaataacgg tttatcttat ttcgatcgat ttgctaatga
    39121 gtcaccgttt acgcatatct ggtctttagc agtagaaggt caaaactatt tggtttggcc
    39181 gatcttgttt gtattgttga tggtatttgt tcgtaaaaga aaatggatct tttatactgt
    39241 gttaggtaca tccttcctct ccgccatctt gatgatgatc ttgtatacac caggaggcga
    39301 tccaacccgt gtttattacg gtaccgacac tcgtctgttt tctatatggt tgggaagtgc
    39361 gcttgcgttt gtttggccaa gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa
    39421 tattctgaat atcgctggag gaatctcgct tttggcactg attctttgct tcttcttttt
    39481 agatgatcat tacaatttca tctattacgg aggaatgctc ctagtgagtc tgttatgcgt
    39541 agtgctagtt gcagtgacag cacaccccgg agcaagtttg aatcgatggc tgacaaatcc
    39601 agtatttact tggattggca aacgaagcta tggcatttat ctgtatcagt ttccagttat
    39661 gattttttat gaagcaaagg tgactgatat tgccgatcat gttttgttgc atactttagt
    39721 ggaaatcaca ttgattctaa tcatcagtga actttcttat cgatatgtgg agcgtccgct
    39781 agcacgtttt gattatggtc gtacattcca agtgatgaaa ggatggttca gcaaaccgat
    39841 catcagtaag agcaaaccat gggttgttcc aggcacatta cttgtagcaa ttgcactcgt
    39901 tggtttcgtt acggcaccga aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa
    39961 aatcgctgaa agtaaaaaat tagcagaaga aaccagaaac aattcagaaa acaccactag
    40021 ttcggttgac caagcgatcc ttgataagta cgagttgtcc aaggcacaag ggaataaggc
    40081 acaaaaatta gaactaacgg catttggtga ttcagttatg ttggatgctg caacggatct
    40141 acaagaactt tatccaaaag tagtggtaga tggagatgtc ggacgacaat tatatgccag
    40201 tgtaccgtac attgaaaaat taaaggaaca agatttgttg aaagacactg ttctgattgg
    40261 actaggaacg aatggctcat tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg
    40321 tgatcgaaaa gtatactggg tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt
    40381 gaatgcgatg cttgaacaga tgacgaaaaa atacgacaat atgacgctga ttgattggta
    40441 cagctacagt aatgaccata gtgactggtt ttatgatgat caagtccatc caaatcctga
    40501 agggatgaca cattacattc acttagtcag cgaagccttg ctaggaggaa gttcaagtac
    40561 gaacagcagt gagacaaacg tgacagaatg atagctacaa aaaacttcag gcaaaaacat
    40621 ttgtttttgc ctgaagtttt tttatattta ccgtgttttt aacattcata tagattcagg
    40681 aagcaaaacg gttgagtgtg tctgtaaata acgttctttt tgttttcggg tgagtttttt
    40741 gaatgcagct tctgcttttg ttgcttcgct tcttgaagaa aatacttctg catggatcat
    40801 cttaacaggt cttctttttt gaagacgagt atatttagca ccagtaccat tgttatgttc
    40861 ctctaatcgt ctagaaagat cggttgtata tccaccataa aatgtctgat ctttacagaa
    40921 taatacgtaa aaataatgtt catttgccat agagcatctc acgaatctct gcagtgtatt
    40981 ctccatccat attgtaagtg atcaatggtg gagcgacttt gaatccgtct aatttgcctt
    41041 ctttgattcc ttcaactaac aaaatatttg cctcttttcc aggttttggg taaacaaatt
    41101 ggatcctttt aggtgcaatt cgatagtctt ccatgacctg aatgatttct aagaaccgat
    41161 caggacgatg gaccatagta aagcgtccat ttgtttttag tagcttactt gaaatatcga
    41221 tcacttcttc taacgtcgta tgaatctcat gacgggcgat tgccaggtgt ttattagggt
    41281 tcgttttatt tgttggcagc cctttgaaat aaggtggatt acagacgact aagtcacaag
    41341 aattatgtcg aacgacagat aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt
    41401 catttaaatg gttaagcgcg atactacgtc ttgccatgtc tgctagacga ggttgaagtt
    41461 cgatagcgtc gatagccgcc tgggttcgtt tacttaaaaa aagtccaact gcgccatttc
    41521 ctgcacaaag gtcaacaatc ttcccttttt taggtacacg ggaaaagtga gcaagcagca
    41581 cagcatctaa agaaaaagaa aacactttgg aactttggat aatcttgata tcttctgcgt
    41641 ataattggtc gattcgttcg tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata
    41701 gtcgtctgta ttatactatt tttattaaaa agaaccaagg tggcttgtca aacctctatt
    41761 ttttatgcat actatgacca taagaaggga aaggaagaga ctatgtttta tcgatttatg
    41821 cgcggggtag ttcgagtact attagcctta gttaatggaa atgcccgcta tgagaataaa
    41881 gaagttttac cgcaagatga gaattacata ctagttgctc cccatcgtac ttggtgggac
    41941 ccattatatc tggcggtagc tgcagcaccc aaggaattca gctttatggc aaaagaagaa
    42001 ctgttcaaaa atccagttct gcgatttata ttaactcgat ccaatgcttt tccagttaaa
    42061 agagaaaatc caggaccaag cgtcattaaa acacctgtaa agattttgaa atcgacaaat
    42121 ttgagtttga tcatgtttcc cagtgggaca agacatgcta cagaacttaa aggcggaatg
    42181 gcattgattt ccagaatgtc taaagcgaaa atcgtgccag ctgtatatca aggtccgttg
    42241 acacttgggg atctgttcaa gcgtcagaga gtgacagtgc gtttcggcga accaatcgac
    42301 ttatcagata tcaagaaaac ggataaagaa ggtatggcgg agatcgaacg acgaacacaa
    42361 gcagcttttg acaaattgga caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa
    42421 aaataaaatg ataagtttat gcgtgggcgg tcttttgaat ctactgtcct gttcagttca
    42481 gactttttca atgaaaacga gggagagacg tcaagtctgc cctctttttg cgttccaata
    42541 tgtaaaatag aagacaggtg tcatgtaatg gatcaaagtg ttataataat tagaaggaca
    42601 ttttttgagg tgattgaatg cgttttttac atacagccga ttggcatatc ggaaaaaaac
    42661 tacaaggtta tgacttgatt gaagaccagc gtcatgtact atcttctata ctagatatcg
    42721 cgaaagaaga aaaagtagag gcaatcgtga ttgccggaga cttatatgat aggagtgtcc
    42781 cagctgttga tgccgtagaa ttatacaatg aattgatggt cgattggaat ttaaaggaaa
    42841 agatcccgat ttttgctata tcaggaaatc acgatagcag tacgagactt tcagcgggta
    42901 cgccatggtt cagttattcg aactattttt tgcgaacaca gctagcagaa gcatttgaac
    42961 cagtcgagtt taaaaacgta caatttttct ttcttcctta ctttgaacca attgcagcga
    43021 gattatattt tgaagatgac aatatccgaa cgatcaagca agcggtagcc aaggtgatag
    43081 ataaaatgca ggagctattt gattcagaaa aaaaacaagt attgattagc catttttttg
    43141 taatgggaag tgtaaaatct gattcggaaa cgaaaatcga agtaggcggg ttggattcga
    43201 tccctggtga actacttaca ccgtttgatt atgtagcact aggacatttg cataatcaag
    43261 ctgctttgag acaggataat gctcgctata gcggttcccc attgaaattc tctttatctg
    43321 agatcaacca aaaaaaaggc gtatggatcg ttgacctgac aacaggcctg gagttgaatt
    43381 ttagggagat cgaacccctg catgatattg aagaaataac agcgagtttc aaagaattgc
    43441 tggatcctgc cttttatcag tccgtcaatc gggagaatta catctatatc tatctcactg
    43501 atagaacgat catcccgaat atgatgaacc agcttcggca aatatatcct cgtattttgg
    43561 ggattgaacg ggcaaacggt cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa
    43621 tgaaagatcc tcaaaaatta gcagaagcat tttttagtga aatgacggaa gagcagctaa
    43681 ctgcaaacca agaaaaatgg ctcggagaaa cgttgcaaga aatcatggaa atggaatgat
    43741 caagaatgaa accgaaaaaa ttacaactta aaaattttgg tccttttatc aatgagaccg
    43801 tagatttttc acgtttgaca gaagcaccgc tttttttgat cagcgggaag acaggagctg
    43861 gcaaaacgac gatttttgat gggatcacct tcgcactttt tggtgaaact tccggaagac
    43921 ttcgctctgg aaaagagatg cgttctttgt ttgctacacc agacgaagaa acaagcgtga
    43981 ctttttcttt tgaacatcag cagatgagct atgaaatcga acgaaaacca gaacaagttt
    44041 tagcaaaacg aaaaggaaac ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt
    44101 ttgatgggaa aggaaaagaa ataaaacaaa tcacaaaacg tacagaagca gatcagttga
    44161 tcagagaact gatgaatctc gatgcgaaac agttttctca aatcgtttta ctaccccaag
    44221 gagaattccg taattttttg gtttcatcaa gcagtgataa agagactgtt ttacgtcatt
    44281 tattcggtac tcaatttttc caacagttca atgagcgatt aaaagaaaaa gcaaaacaac
    44341 agcaacaaaa attggatcat ttagagcaag agctgcatct tctgcaaaaa aggtttgttt
    44401 tagtcaaaga agaggaacca gcagcagggt ttgaagccgt tttagcccaa tggacaaacc
    44461 atcaggcaat actcgaaaaa caaatcacgg aagaaaaaga agctctaacg gaacttcagg
    44521 aaaaacaaaa gcaactagaa aattcctact atcgttttga aagcttgaaa aaaagttatg
    44581 aagaaaaaac gatattaata aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag
    44641 aggaaaagaa acgatggatt gggtattatg aatttacgca acgattagtc gaaccaatcc
    44701 agcgcctaca agaaataaaa gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa
    44761 caaagaaact agcgatgact acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc
    44821 gcgtacgccg acaaacaaaa atcaaagaag ctgctcaaaa actgcaaaac aaacagatga
    44881 tggtgccaat tgtcgaatcc ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt
    44941 cattggataa agaagaattg aatgccgaga agctgtatca aaatctcgct atctatcagg
    45001 aaagaactcg aattcttcaa gaaaatttac tggatcagga aaaactgcaa gaagatcgtc
    45061 ttgcttttgg acagttaaaa tatctagatg aaaagcagca agaaatagaa aaacaaaaag
    45121 caaaagacac tgggtcgctc gcacagataa gagaagaact caaaaaagca actgaagaaa
    45181 taacaaaaaa tagtcagcta ttgaaagaac agacagaaca agcagaacag ctgaaaagcc
    45241 aatgggcaaa gcagcaaatt gctcgtcttc agcttttttt gcgtccaggg gagccatgtc
    45301 ttgtttgtgg atcaacggaa caccccatac aacaattaaa acacgaacag ccaaataagg
    45361 aagatatctt aaaagtagaa caacaactgg cagaagctga gtctgctgta gaaagaactc
    45421 agcaaatcat tgccaaaagt gaagcacatg aaaataatct tcaacaacaa ttatcagatt
    45481 tagaacaaaa aatcatggca acgaatatac aattggctga acaagaagaa cacttgcaac
    45541 aggctttgaa aaaggcattt cctgagataa cagaacaaaa aacaaaaaca actcttgtag
    45601 taatcgaaca atctttagat agacgaaaaa gcaagatggc caatgaaaga atagagctgg
    45661 aagaaataag acaacaagca gaaaagctcg aacaacaatt agctgtctac aaacagcagc
    45721 agacagaaaa aaataatcag ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac
    45781 aattgcaaga tacggatagt aactgtttag aagaggagat acgacgactt gaaaaacagt
    45841 tagaagaaga ctcgaatcaa ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag
    45901 atcttcatac gcttcggaca cagcttgatc ttcaacatga acaaatagaa aaaatagatg
    45961 aaaaaataga aaaggaacaa gaaagaatag ctaaaaagtt agaaagccag tcgtattttt
    46021 caacaattga agaaatctgt gcatttgcac aacagcaagc acaatataaa gaaaaccaac
    46081 gtacagttac ttcttatcat gaagagcaat tagtgacagc tgaccggctg cgtcaattag
    46141 aaaatatcca gctaactgag acattcccag atacagaaga gatcagccaa cagttagaag
    46201 aaataagacg tatgatcgaa ataaagcaag aaaagatata cggtttacaa gaacaaaaaa
    46261 ttagtaatca aaaaatctac gatgaatgtc tttcgattta tcacacaagc cagacgaaat
    46321 tagatgagct tacgcaatta cagcagcttt cgcaaacatt caacggagaa aatcctaaga
    46381 agactagttt ggaacggtat gtgcttcagg tttatcttca agaagtgtta caagtagcaa
    46441 atgatcatct ccaacgttta acgaaaaatc gttatcaatt tgagctagcg gataccattg
    46501 gcagttatcg agggaaaacc ggattagaaa tcaatatcta tgatgatcag gcaggtatgt
    46561 ctcgaagtgc gcataccctg tctggcggtg agagttttat tgcagcactt tctttagcat
    46621 tagctcttgc cgatgtgatc cagacacaag ctggtggtgt agcaatcgat gcattgttta
    46681 ttgatgaagg atttgggtca ttagatgaag aagctttgga aatggcaatg gaagctttag
    46741 aaacgatcga aagtgaagga agaatgatcg gcatcattag tcatgtaaga gaattgaaag
    46801 aacgagtatt acaacaaata agaattgata cagaaggtag cggacaaagc aaaatacgct
    46861 atcaacttgg ataaaaaggg agattggtaa ctatgaaatg gagtattcca gaaaaagtca
    46921 ttgcacgtgg acgtcaatac ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag
    46981 aagaaaacgt gtggcatgca gaagtcttgg gcagtgaatt gtatatggtt gatttggatg
    47041 gtacagcaaa agagatagat tactgccaat gtccctattg ggaggaacat aaatattgta
    47101 agcatacagt tgctgtggaa ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa
    47161 aagaccagcc aatgctagaa ccggtaaaag caacaagtga gggagaaatc attgcaaaag
    47221 gtttttctcg tctaaaaaca caaaacaatc ctcaagcggt gcaacctttg attattgaat
    47281 accaagtaga aacgattgag actaatcagt atcacccaga gctttctatt ttggcacttt
    47341 acttgcgtat cggttcattg gcatcaccga aaaaaacata tatcgtaaaa aacatctatg
    47401 attttttaca agcttacaca gaaaaagaaa cttacactgt taataaacag taccagtttc
    47461 gtctagataa acgggcgttt caaaaagaag atcaaaaaat actcacgcat cttgcaggaa
    47521 gtgctcagac gcatcaatta ttaggtacga acggcttaca agtcaaagga aaactagata
    47581 aaagatatct gcttctccca gtagaacaag cccgcttcct gttagagcag atgaatcaga
    47641 ctacacgttt gcatctgcag ataaatgaac aaaacgatca aggaatacaa ttttctgatc
    47701 aaaacaaacc tttatcattc aaagtggaaa aatttggaga agattatttg ctgcaagcat
    47761 tgaatgattt tgattttttc tttatgcatt atcagtgggg agttatccaa gggacatttt
    47821 attctctgac gaaatatcaa caaacggttt atcaaacttg gaaacaattg attcgccgat
    47881 tggagaaacc agaagtagtg ttcaagaaaa aagaattacc gtctttgttt aaagagatta
    47941 tccctctgct gtcagagatc ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag
    48001 cagatatccc cgtcgaattt attttctttt tccgaaaagc aaaaggaaaa atccaagctc
    48061 gagtcgattt cgtctatggt gaagtcatct attcgactga cccgaagcat gaggtctcga
    48121 cagaacaggc ttttcaggtg ttacgtgaca cagtagaaga gaaacgagta cttgatctat
    48181 tcagaatgta tcgctaccag aaaaatgaaa ctggttatga acgtccattg ccaagcgggg
    48241 aagaactata tgcctttttc cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca
    48301 gacttggaag aaaattgcga gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg
    48361 aagtaaccga gaccggttca tggctcgata ttcgattcga tgtgacaggg atagaggagc
    48421 aagaaatcga tcatgtcctg caaagcttgc tgagaaatga tgctttttat acattagaaa
    48481 atggtcaggt tctttctttt gattcagaag aatttcaaca aacaagccaa gtgttgcaac
    48541 aattacggga atcgatacgt acagaagaag gaacgatcca tgtacctaaa aatcaaggat
    48601 tgatcattca aaatcaattg gaaaaaagca atgctacttt tagtgaatcg ttccaaacga
    48661 tggtacaaga tctcattcat ccagaaagat accaggcaca gcttcccaaa ggattgaatg
    48721 caacgatgag agattatcaa aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc
    48781 agtttggcgg tattttagcc gatgaaatgg gacttgggaa aacactacag acaattgcct
    48841 ttttactttc ggaaaaagaa gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga
    48901 gcttaattta caactggcag gcagaggtga gaaaattcgc cccttcttta agtatccaag
    48961 taatcaatgg gaataaaaaa gaacgtgaag aattactggc taaagacaca gatatccgag
    49021 tgacttctta tgccagctta agacaggatc tagctaatta tcagtcgcaa aaaattgatt
    49081 atttgattct tgatgaggca caaatggtca aaaatagcag tacgaaaact gcacaagcat
    49141 tgagagaatt agctgtacct cagcggtttg cactgagtgg gacaccaatc gagaataatt
    49201 tagaagaatt gtggtcattg tttgcaacga ttatgccagg atttttcccg actaaaacaa
    49261 aattcaggga acttgccgca gaagaaattg cgcaaatgat tcgaccgttt attttacggc
    49321 gagataaaca aactgtgttg aaagacttgc cggaaaaaac agagatgaat ctatattctg
    49381 cacttacaga agaacaaaaa actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa
    49441 tcacatcaat ggattctgaa gcattcaaga aaaaccggat cgggatactt gctggtctga
    49501 caagactgcg acaaatttgc tgcgatcctc gtctgtttat tgaagattac caaggcggtt
    49561 ccggcaagct cgaacaagta aaagatctac tggtagctgc caaagagaac aaacgaagag
    49621 ttttgctgtt ttctcaattt acagggatgc tgactatctt gcaagaagag ttagccgagc
    49681 tagggatctc gacattttat ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg
    49741 tagacgcatt taatgctggc gaaaaagatg tttttctgat ttccttaaag gctgggggaa
    49801 ctggattgaa tctcacggga gcagatacgg tcatcttgta tgacctttgg tggaatcctg
    49861 ccattgaaga acaagctgct ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag
    49921 tgtggcgtat gatctctgaa ggaacgattg aggaacgcat ggattcactc cagcaagaaa
    49981 aacgcgaact gttccaaaaa gtgatccaag gtaatgaaga acagttgacg aaactgactg
    50041 aagaagacat ccgcatgatt ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa
    50101 gcgttcagta atgggtaaga aagcctttct aaagaagcga ttgtttattt gatttaaaaa
    50161 atagcaagga ggctgggatt ccattttatc ccagcttcct tgctatcatt tttttatttc
    50221 tatttttaaa ggttatacaa agattgtctt cattgtctct aacagctgat caaaagaaga
    50281 aatctcgata tcgcaaattg gagtctgttc aattttgcgt tctcgatggt tgaaccaaag
    50341 cgcttgccag ttagcacttt ttgcacctag aacatcattg tcgtaattat cgccaacgta
    50401 taaggtctct tctggaagca tatgaaattc tttctcagcc agctgaaaaa tctctttttc
    50461 tggtttttgg aaacctgttg cttgagaaat gagcatattg cgggaaggaa tccaattgtt
    50521 caattgaagt tgcttcagtt tttttgtctg atggtcagtc ggcccatttg tgataattcc
    50581 taatgggaca ttcttttcct tcaagaaatc gagggttttt ttgacctcct catgcaagca
    50641 gatattatca agttcttcct cgtaaatttt ttgaaataag aggccatctt cttccgtaat
    50701 atgagggtag tctaaatctt ttagtgattg gctgatccga tgtgctcgca tatattctag
    50761 tgtccaatct cctgccatta cttttgggaa gttttcatca ctatgatggc ggaaccggat
    50821 atatagtgga tgcatatctg catctgaaac aagcggaacg actcttttga ccgcgtttcg
    50881 aaatggctgc tgctgatcat acatcgtatc atcaacatca aacacaactg atttaatcaa
    50941 aattttacac gcctttcggt tttcattgat tttttcacaa atccttgact attgtaacag
    51001 aaaaaattgt ctaaaaaagc tgataaataa aggtttttct tactatattt ctatttcctt
    51061 gacagaataa ggtttttata tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata
    51121 agtttgatga acaaattaat cattatggta gaagtcgatt gatttatttt aatgcaatct
    51181 ctgtggaatt ttcgaaagat ttattttttt aatacagtct caatataact attcaaacat
    51241 aaaataacgt tatttgattt atttgtttat agtaaatttt atttagtaa
//
LOCUS       DACDTH010000011        51179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDTH010000011 DACDTH010000000
VERSION     DACDTH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.011"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            567..1404
                     /locus_tag="IWK54_000734"
                     /pseudo
     CDS             567..1404
                     /locus_tag="IWK54_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            1469..1903
                     /locus_tag="IWK54_000735"
     CDS             1469..1903
                     /locus_tag="IWK54_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9592879.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(1971..3293)
                     /locus_tag="IWK54_000736"
     CDS             complement(1971..3293)
                     /locus_tag="IWK54_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9592880.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3447..5087)
                     /locus_tag="IWK54_000737"
     CDS             complement(3447..5087)
                     /locus_tag="IWK54_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9592881.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            5417..5887
                     /locus_tag="IWK54_000738"
     CDS             5417..5887
                     /locus_tag="IWK54_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9592882.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            6516..7619
                     /locus_tag="IWK54_000739"
     CDS             6516..7619
                     /locus_tag="IWK54_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9592883.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            7903..8583
                     /locus_tag="IWK54_000740"
     CDS             7903..8583
                     /locus_tag="IWK54_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9592884.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            8673..9707
                     /locus_tag="IWK54_000741"
     CDS             8673..9707
                     /locus_tag="IWK54_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9592885.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            9721..10056
                     /locus_tag="IWK54_000742"
     CDS             9721..10056
                     /locus_tag="IWK54_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592886.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            10040..12520
                     /locus_tag="IWK54_000743"
     CDS             10040..12520
                     /locus_tag="IWK54_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592887.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            12576..14135
                     /locus_tag="IWK54_000744"
     CDS             12576..14135
                     /locus_tag="IWK54_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9592888.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            14132..15496
                     /locus_tag="IWK54_000745"
     CDS             14132..15496
                     /locus_tag="IWK54_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9592889.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            15667..16737
                     /locus_tag="IWK54_000746"
     CDS             15667..16737
                     /locus_tag="IWK54_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9592890.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            16752..17420
                     /locus_tag="IWK54_000747"
     CDS             16752..17420
                     /locus_tag="IWK54_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9592891.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            17440..18018
                     /locus_tag="IWK54_000748"
     CDS             17440..18018
                     /locus_tag="IWK54_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9592892.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            18421..19332
                     /locus_tag="IWK54_000749"
     CDS             18421..19332
                     /locus_tag="IWK54_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9592893.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(19588..20505)
                     /locus_tag="IWK54_000750"
     CDS             complement(19588..20505)
                     /locus_tag="IWK54_000750"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9592894.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            20707..21144
                     /gene="zapA"
                     /locus_tag="IWK54_000751"
     CDS             20707..21144
                     /gene="zapA"
                     /locus_tag="IWK54_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9592895.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            21202..21749
                     /locus_tag="IWK54_000752"
                     /pseudo
     CDS             21202..21749
                     /locus_tag="IWK54_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            21864..24224
                     /locus_tag="IWK54_000753"
     CDS             21864..24224
                     /locus_tag="IWK54_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9592896.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            24311..24625
                     /gene="trxA"
                     /locus_tag="IWK54_000754"
     CDS             24311..24625
                     /gene="trxA"
                     /locus_tag="IWK54_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9592897.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            24807..26585
                     /gene="uvrC"
                     /locus_tag="IWK54_000755"
     CDS             24807..26585
                     /gene="uvrC"
                     /locus_tag="IWK54_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9592898.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            26730..27629
                     /locus_tag="IWK54_000756"
     CDS             26730..27629
                     /locus_tag="IWK54_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9592899.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            27622..28884
                     /locus_tag="IWK54_000757"
     CDS             27622..28884
                     /locus_tag="IWK54_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9592900.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            29047..30945
                     /locus_tag="IWK54_000758"
     CDS             29047..30945
                     /locus_tag="IWK54_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9592901.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(31244..31963)
                     /locus_tag="IWK54_000759"
     CDS             complement(31244..31963)
                     /locus_tag="IWK54_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9592902.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            32300..32632
                     /locus_tag="IWK54_000760"
     CDS             32300..32632
                     /locus_tag="IWK54_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592903.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            32619..34622
                     /locus_tag="IWK54_000761"
     CDS             32619..34622
                     /locus_tag="IWK54_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9592904.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLMP
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            34641..35111
                     /locus_tag="IWK54_000762"
     CDS             34641..35111
                     /locus_tag="IWK54_000762"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9592905.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            35132..35716
                     /locus_tag="IWK54_000763"
     CDS             35132..35716
                     /locus_tag="IWK54_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9592906.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            35731..36717
                     /locus_tag="IWK54_000764"
     CDS             35731..36717
                     /locus_tag="IWK54_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9592907.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            36707..37018
                     /locus_tag="IWK54_000765"
     CDS             36707..37018
                     /locus_tag="IWK54_000765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9592908.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            37040..38821
                     /locus_tag="IWK54_000766"
     CDS             37040..38821
                     /locus_tag="IWK54_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9592909.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            38814..40190
                     /locus_tag="IWK54_000767"
     CDS             38814..40190
                     /locus_tag="IWK54_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9592910.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            40197..40832
                     /locus_tag="IWK54_000768"
     CDS             40197..40832
                     /locus_tag="IWK54_000768"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9592911.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            40862..42217
                     /locus_tag="IWK54_000769"
     CDS             40862..42217
                     /locus_tag="IWK54_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9592912.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(42409..43803)
                     /locus_tag="IWK54_000770"
     CDS             complement(42409..43803)
                     /locus_tag="IWK54_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9592913.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            43895..44866
                     /locus_tag="IWK54_000771"
     CDS             43895..44866
                     /locus_tag="IWK54_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9592914.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            44863..45645
                     /locus_tag="IWK54_000772"
     CDS             44863..45645
                     /locus_tag="IWK54_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9592915.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(45814..46185)
                     /locus_tag="IWK54_000773"
     CDS             complement(45814..46185)
                     /locus_tag="IWK54_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9592916.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            46319..46762
                     /locus_tag="IWK54_000774"
     CDS             46319..46762
                     /locus_tag="IWK54_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9592917.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(46805..47614)
                     /gene="yidA"
                     /locus_tag="IWK54_000775"
     CDS             complement(46805..47614)
                     /gene="yidA"
                     /locus_tag="IWK54_000775"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9592918.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(47633..49000)
                     /locus_tag="IWK54_000776"
     CDS             complement(47633..49000)
                     /locus_tag="IWK54_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9592919.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(48984..49814)
                     /locus_tag="IWK54_000777"
     CDS             complement(48984..49814)
                     /locus_tag="IWK54_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9592920.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            49977..50414
                     /locus_tag="IWK54_000778"
     CDS             49977..50414
                     /locus_tag="IWK54_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9592921.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            50490..51110
                     /locus_tag="IWK54_000779"
     CDS             50490..51110
                     /locus_tag="IWK54_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9592922.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        16705 a         8868 c        10794 g        14812 t
ORIGIN      
        1 caaaagaatg aattaataag atataaaaaa agggattgtg acaaacgtct tgtcacaatc
       61 cctttttcca aataaacgat gttcaatcag ctgcccctcg gcattcgctc gaaaaacggc
      121 gaaatatgaa gaactatttt gccatttgtt tctctaataa tcaggtcaaa acgttgactt
      181 cacagtctct tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt
      241 caacaaacat gagaagctgt tttttgaaat tgttccttgt gactcggagc tgaacggcag
      301 gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagttcaac
      361 caaacgaaaa ttatgaggag ctatttttgt ttggctgttc ttattacttg atgcaggaca
      421 gctttttcac aacctctttt cgtttagcac taaatttcct taaaaagtag aaaagctttc
      481 gaagcctcgt ttgtttcttt tactgtataa taaaagaaaa gagtaacaat cgctaaaaga
      541 tataaacaag agaaaggaaa tacaagatga ttggtctgat ttattttgtg gtgatcgttc
      601 tggcgaacac agttggcgct atttccggga tgggtggagg cgtgttgatc aaaccgattc
      661 ttgatcttat cggcgcccat tctgttgcag ggatttcttt ttactcaaca gttgcggtat
      721 tcacgatgtc cattgtttcg actgtgcgac aagtatcaag cgggaaatca ctgaattggc
      781 agattgtcgg ttgggtatct agtggagctg ttgttggagg agtagtcggc aacattgtat
      841 tcgaagtttt tttacagtta tttgaaaacg agaagcatgt gcagctgatc caaatttttt
      901 tgacggtatt gaccttggtt tttgcgttct tctacaccaa gcatcatcag ccaaaatttc
      961 atttgacgtc atggacttgg taccttatat gtgggggggg ttctgggctt tttagctagt
     1021 tttttaggta ttggtggtgg accagtcaat gtttctttgt tgatgctgat gtttgcattg
     1081 ccaataaaag aagcaacact atattctcta tctacgattt tcttttctca actggcaaaa
     1141 ttggtaacga ttgctttaac aagtagcttt atgcgctttg atttatctat gttgttttat
     1201 gttatccccg ctgctgttat tggaggtttg tggggagcaa gatttagcag ggttttgtct
     1261 ccaaaaaaag tgacctttat ttttcaagta attgtcctcg tggttctttt ggtcaattgt
     1321 tacaatgcct ttgtcatctt acaaacatta ggagaatcta gactaacgca agtcaaaatc
     1381 actgaaacat attgttctaa atgaaaaaaa gagtatacta tgataaaaaa attcatttta
     1441 aaaaatcaga aattggctgg tgatttggat gttacgggaa atgcgtttag aagatgcaga
     1501 agcactacgt gaaatcaatg cggatcaatt agggtatgat ataccattaa ccgttacggt
     1561 gagacaaatg aagaaactgc tgaaggagcc agagaaaaat tttttcttgg tttatgaaga
     1621 tcctacttct aaaaaagcag ctggctatgt acaagcggag tcctatgaat cgatttttag
     1681 cgatccaatg ttcaatatta tggcacttgc tgtgtctaaa tatgctgaaa aaagaggcat
     1741 cggaaaggct ttgatgactg gcgtagaaga agaagcgaaa aaacgaggaa taacgacaat
     1801 aaggcttaat tctgcagaat acagagtaga agcacaccaa ttttatgaac acatcggtta
     1861 tcattccgat aaaatgcaaa aaagattttt gaaaatcctg taaatcttta tcaaaaaaat
     1921 acccgatcac caagccgata cttgatgatc aggtattttt gtttttttga ctaagaaagt
     1981 aattgcatca agaaaccaga gatgatcaaa acgatggcac caccaagtcg attgcccatt
     2041 tgagcaaaag caataagttc catccgatta gctgcagata agacggctac atttcctgtt
     2101 ccgcccatcg agttgttgca taagccagca gtaatagttg attcaattgg atataaacca
     2161 aacaaacgac cgacaaatcc tgaaacaact gaaatcgcga taacacttgt cagacataag
     2221 acaacaaatt gccatgtcaa agcagaagct aatacattca gattcagcaa cgcaataccg
     2281 ataccggcaa gaacagctgc tgtcaagttt ttgacaatca agttattgaa catgatagct
     2341 gcatcttcat aatactctgg taataggttg gcgattttac agatgacaac gataataatc
     2401 ataaatgcgt aagcatggac tttaggaaca aaatagttac caatttgtcc taagatgaag
     2461 aaagtcaatg ataccatcaa accaacacct aactgtggaa tggaaggttt cacagttcga
     2521 ggttttccat cgccaagatc gcctttttcc attaatttac cattgccgtt gaattttggc
     2581 attgattgtc cagcacgagc aactaatgct gcaccgatga tagctaagac atttgtcatt
     2641 gctgtcgcag gaatcaggcg ggaaatgata gaagaagcat ccgtacctaa aacatttgca
     2701 taaatcgttg ataaaggaac ggctccggct ccaacaccac cagccatcgc aggtaatgag
     2761 atgtataaaa cggaatttgc aaaaccattt ccgataagca tgcccacgag tcctacggca
     2821 aaaaaggaga cgaccattga aataagtgca acgggaataa aacgaacaga agctttcatc
     2881 aatagattac ggttcatacc aaggatgcta ccagtaatca aggcagcaat ataaaagtcc
     2941 caaaagccca tattgctgac aaagttttct gtggatttca ctacatattc aggtaagatc
     3001 ccaaatgtag caatcgcagc agaagcgaag atcgcaaaaa cggaaccacc acccaaataa
     3061 cttttgacga tagggatttt tgtcccgata aaatgaaata aattaccgaa aataaccaaa
     3121 actgcgattg gtccaatcat attcgtcggt aatttcccta aagccattgc aatgatcagg
     3181 acaaggacca tgatcaaata aagtggcaaa cttacgccgg tgatttttgt ggaccaaaaa
     3241 ccttcttttt tcttttcctc aactgcagtt gcagttgttg ttttttgtgc catttttaca
     3301 gatcctttct tattctgtat catatgaaaa aagccaaaga aaagcgacaa aagagcagga
     3361 gttggctggc cgctgttttg ccgcagtcgc ttttttcttt ttagacgtca tacaaagttt
     3421 ctgtatgttc ttttactata acgatcttaa tcttctaaag atttatattc aggaacccat
     3481 ttagcagatt caactgcttc tttgatatcc gtgatttctt cacgattcaa gttttgtgcg
     3541 acaactgatt ttgcgacagt ttcagcaatc gtttgagaaa attcagtgat ttttgctact
     3601 ggaggcaaga tagctgctcc tggttttgtt acatcaacga ttccgcctaa tgctcgacta
     3661 gcttgagaaa tgatttcagc atttactcgt gtagctgttg aagcaatcaa accaagtcct
     3721 aaacctgggt acatcaaagc attatttgct tgaccgattt ggtaagttac accattatat
     3781 tcaacatcag cagcagggat acctgttcca actaatgctt ttccgtccgt ccattctatt
     3841 aaatcttttg cttttgcttc tgccaatttt gttggattag acaaaggcat gatgatcgga
     3901 cgtggtgtat gagcagccat ttctttcacg atttcttcag tgaatgctcc aggttgggta
     3961 gaagtaccaa tcatgattgt cggatggatc gcttttacaa ctgcttctaa gtttgtcaat
     4021 tcatcagcat tagtgaaatc tgagcgtttg cgagcgaatg ggatttgacc aggagtcaat
     4081 ccttctgtat cttcaaacaa taaaccttgt ttatctactt gatagaaatg ttggcgtgct
     4141 tcttcttcag gtacaccttg acggatcatc tcatctaaaa ggatgtttgc gatacccaca
     4201 ccagcagtac cagcaccaaa tgtcaaaatt atttgatctt tcaattgttc accggatata
     4261 ttcaatccgc ctaatacacc tgctaggaca acaattcctg taccttggat atcgtcgttg
     4321 aaagtagtga tttgatcttc gtatttttct aagatcgctg cagcatttcc tcgtccaaaa
     4381 tcttcccaat gcaataacaa ttctgggaat aattcggtag ctgattgaac gaattgatcg
     4441 atgaattcat agtaagtttc gccttctaca cgagcatgac gattgcctaa gtataaggga
     4501 ttattcaata attcttcatt attagtacca gcgtcgatcg atacaggcaa aacttgtgct
     4561 ggattaatac cagcagcagc agtatagacc atcaatttgc cgatagcgat gtcaacaccg
     4621 ttcacacccc agtcgcccat acctaagatt ccttctgcat cagtcacaac aatcaaacga
     4681 atgtctcgtc catctgccgc gttcttcaaa cttgctttga tgttttctgg ttcgtcgatt
     4741 gaaagaaatg cagcatcttg cggtttcaag aaaatttcgt tgtattgttc gattgaatcc
     4801 gcaacgacag gatcataaac gacaggcatg aattcaacta aatgttcgcc catcaattta
     4861 taaaacaatg tccgattcgt attgaataga ttcattaaga agatacgttt ttccaaatct
     4921 gtttgtttgc ttaagtattg accataagct tgaacagatt gttgttccag tgtttggaca
     4981 gtacttggaa gcataccagt gattccatat tttgcccgtt cttcttttgt aaaagctgtt
     5041 cctttattta gaaaaggatt atttaataag tttaaccctg taagcatgaa tgattcctcc
     5101 taagttttaa tacaaacgaa gtatagaagt cagatgatga tatagtcaaa aagagcaggt
     5161 atgataaatt ttatttatat gtaatatagc caaaagaaat caaaatagca gtatttgttc
     5221 tttttatatc gacaatcctt gcttgaatac tatgcgtatc tcttatatgc tagagaaaga
     5281 aaagtagaag aataagggat agatggaatg gataaatagg atttctgagg aaaaaagcac
     5341 atgatagcgt tgataggagt cacctatgtc aggtgataaa aatacataag tagaaaatta
     5401 agaggaggaa gagaaaatga aagaagttca attgattaga aaaagcgaac tatcagaagg
     5461 cggttgcaat gcatgtggcg tagtagaagc gacaagctat acattaaaat tagattccaa
     5521 tcaagcaata atctctgaac tgacagtagg cggtcttgtg gacagtctgg cgcttgctga
     5581 aggttttact ggagaagata tttatgagat gttttcagaa gtccgtcagt taaaaaaagg
     5641 agaaaaatgc atagaggttc atcatgagag cccgaatgtg aggttcaaac gtggtgataa
     5701 tgagatgatc ttcaagaatc atgtgtctga tcacactgaa ctatatgaaa tcgtgaatca
     5761 aatactgtct gggatttttg aattaggacc ttatgagttc aaggaagaaa acggtaatcc
     5821 aaaattaaat gaagaatggc aagaaacgat tgaaacacaa agaaacaatc cacacttatt
     5881 tcaatagtct tccacagtat ttcgtaaaaa tgttgatgaa aaagaaacac cgtgaatcag
     5941 aggtcgtgaa aaacctgccc tgcatcaagt aataagaaca gccaaacaaa aatagctcct
     6001 cctatttttc gtttggttgg gcttattacc gcagatgcaa gtttttgaac accgtttaag
     6061 gaagagactg tgaagtcaac gttctgacct gagtattaga gagacaaatg gcaaaatagt
     6121 tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga acaccgtttt
     6181 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
     6241 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
     6301 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
     6361 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
     6421 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
     6481 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
     6541 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
     6601 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
     6661 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
     6721 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
     6781 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
     6841 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
     6901 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
     6961 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
     7021 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
     7081 tcccgctgcc aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
     7141 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     7201 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     7261 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     7321 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     7381 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     7441 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     7501 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     7561 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     7621 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     7681 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     7741 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     7801 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     7861 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     7921 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     7981 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     8041 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     8101 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     8161 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     8221 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     8281 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     8341 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     8401 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     8461 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     8521 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     8581 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     8641 ccgcagttgt tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt
     8701 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     8761 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     8821 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     8881 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     8941 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     9001 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     9061 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     9121 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     9181 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     9241 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     9301 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     9361 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     9421 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     9481 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     9541 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     9601 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     9661 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     9721 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     9781 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     9841 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     9901 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     9961 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
    10021 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
    10081 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
    10141 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
    10201 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
    10261 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
    10321 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
    10381 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
    10441 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
    10501 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
    10561 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt
    10621 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
    10681 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
    10741 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
    10801 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
    10861 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
    10921 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
    10981 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
    11041 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
    11101 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
    11161 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
    11221 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
    11281 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
    11341 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
    11401 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
    11461 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
    11521 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
    11581 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
    11641 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
    11701 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
    11761 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
    11821 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
    11881 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
    11941 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
    12001 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
    12061 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
    12121 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
    12181 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
    12241 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
    12301 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
    12361 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
    12421 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
    12481 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
    12541 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
    12601 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
    12661 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
    12721 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
    12781 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
    12841 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
    12901 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
    12961 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
    13021 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
    13081 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
    13141 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
    13201 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
    13261 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
    13321 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
    13381 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
    13441 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
    13501 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
    13561 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
    13621 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
    13681 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
    13741 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
    13801 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
    13861 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
    13921 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
    13981 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
    14041 ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt
    14101 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
    14161 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
    14221 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
    14281 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
    14341 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
    14401 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
    14461 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
    14521 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
    14581 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
    14641 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
    14701 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
    14761 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
    14821 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
    14881 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
    14941 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
    15001 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
    15061 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
    15121 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
    15181 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
    15241 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
    15301 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
    15361 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
    15421 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
    15481 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
    15541 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
    15601 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
    15661 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
    15721 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
    15781 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
    15841 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
    15901 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
    15961 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
    16021 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
    16081 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
    16141 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    16201 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    16261 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    16321 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    16381 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    16441 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    16501 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    16561 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    16621 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    16681 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    16741 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    16801 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    16861 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    16921 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    16981 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    17041 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    17101 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    17161 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    17221 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    17281 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    17341 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    17401 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    17461 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    17521 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    17581 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    17641 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    17701 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    17761 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    17821 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    17881 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    17941 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    18001 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    18061 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    18121 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    18181 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    18241 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    18301 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    18361 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    18421 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    18481 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    18541 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    18601 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    18661 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    18721 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    18781 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    18841 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    18901 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
    18961 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
    19021 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
    19081 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
    19141 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
    19201 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
    19261 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
    19321 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
    19381 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc
    19441 tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga
    19501 ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac
    19561 tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt
    19621 attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    19681 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    19741 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    19801 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    19861 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    19921 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    19981 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    20041 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    20101 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    20161 acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc
    20221 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    20281 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    20341 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    20401 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    20461 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    20521 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    20581 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    20641 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    20701 attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact
    20761 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    20821 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    20881 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    20941 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    21001 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    21061 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    21121 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt
    21181 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    21241 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    21301 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    21361 attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct
    21421 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    21481 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    21541 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    21601 tttttgattt tgcagttact tgcctttgtt ccattagtgt ggtccaaaat caattcggcg
    21661 agcatagttt cgctcgtttt atcgttgaga gaacgccatt tcttaccaac aagatgtatg
    21721 agctttgggt aacgaatatt ttgaattaag gaaaatagtg ctggaaaata tttcgttttt
    21781 tgtacagagt agtttattta agaaggctgc gtagcgtatg tccggcacag ctcttttttc
    21841 atagtgaaag ataaggtgat aaaatgaata accgaatcct tgaaacattg gaatttgaaa
    21901 aagtcaaaca gatggtcaga caatttgtag tgactgcaca aggaaaagaa gaactggcag
    21961 aattggtacc agtgagtgag aaacaaacga tcacaaactg gcttcaagaa actgaggatg
    22021 gattgaaagt gcaacgtctg cgaggcggga tccctatacc taaacttgaa aatatccgtc
    22081 cccatatgaa acggatcgag atcggagcag acttgaatgg agtggagctt gcacaagttt
    22141 ccagagtatt gtctacgaca agcgaattga atcgtttcat tgatgatctt tctgacagtg
    22201 aaatcgaatt tgcacgtttg tatatgtggg cagatcagtt agtcacgatc cctgttttga
    22261 gccgccgttt aaaggaagca atcgatgaag acggacgcgt gactgatgac gcttctcctg
    22321 agttaaagag cataagacag aacattcggc gaagtgaaca agctgttcgt gaacagttag
    22381 atgggatcgt ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc accatgcgaa
    22441 atgaccgcta cgttatccca gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc
    22501 acgatcaaag tgcttcagga cagactttat ttattgaacc aaaacaagtg gtggatttga
    22561 ataatcgttt gcgtcaatac caaatcgctg aacgaaatga gattcaacgt attttaagtg
    22621 aactttctgc tgaacttgtt ccccatcgtc aagaaatcat ccacaatgct tatgtgattg
    22681 gtaaaatgga tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc
    22741 cgggaatcag tgaagacaat catgtcgtct tgaaacaagc aagacatcca ctgatcgatc
    22801 aggaaaaagt agtttctaat gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta
    22861 caggacctaa cacaggtggg aaaacgatca cgttgaagac tttaggtctg ttacaactga
    22921 tggggcaagc aggattaccg attcctgcag gtgaagaaag tcaaatcggt atttttgagg
    22981 aagtatttgc ggatatagga gacgaacagt cgattgagca aagtctatcg accttttctt
    23041 ctcatatgac gaacactgta gatattttat caaaagtcaa tgaaaaaagt ctggtattat
    23101 ttgacgagct cggtgcagga acagatccgc aagaaggtgc tgctctagca atcgcaatct
    23161 tagatgatct tggtaaaaag tctgcttatg tgatggcaac aacgcattat cctgaattga
    23221 aagtttatgg atacaatcgg gcaaatacga ttaatgccag catggagttc gatgtggata
    23281 cactaagtcc aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa
    23341 tctctagccg attaggtctt gatacagaag ttattgatga agcgaagcag ttgatgaatg
    23401 atgagagcca agatttaaac gaaatgatca cagatttaga gaatcgccgg aaaatggcag
    23461 agaccgaata tctggaaatg cgtcattttg tttctgaggc acaagaactt cacgatgatt
    23521 tgaaagaagc ttacagctat ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga
    23581 aaaaagcaaa cgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc attgctgata
    23641 ttcgtaaaat gcaacagcag attgggcaag gaaatgtgaa agagcatcag ttgatcgatg
    23701 caaagaccca attagcaaat ctacatcaag aagaaacttt gaagaaaaat aaagtattga
    23761 agaaggcgaa agaacaaaaa acgttgaaac cgggagacga agtgttggtt acgacttacg
    23821 gccaaagagg gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat
    23881 taaaaatgaa tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa gagccgacac
    23941 aacgagttat ccatgctgta cgatctgaat ctagtagtca cgtgccgaat caattggacc
    24001 ttcgaggaaa acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg gattctgcca
    24061 ttctagctgg ttatcctcaa gtgactattg tccacggaaa aggaacaggc gcacttcgca
    24121 agggaattac tgattatctt aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg
    24181 caaaccaagg cggaaatggt gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa
    24241 gcagatggtt cgctatccaa gtatagaaat gctatactaa tcatagattt taaaaatgga
    24301 ggtctttgat atgtcacaag taattacaga taagacattt aatgaagaaa cagataaagg
    24361 acttgtattg attgattttt gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat
    24421 tttggatcag ttggaacaag aatatgatga agaagaattc agaattgcta aaatggatgt
    24481 ggatgaaaat ccagaaactc ctcaacaatt cggtatcatg agcattccaa cattaatgtt
    24541 gaaaaaagat ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag aacaactacg
    24601 tcaaatgatc gatcaatatt tataaaaact tacagcaaaa ataagctgag ggtgggactg
    24661 aatccagttc cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa ttaatatttt
    24721 ttgagtataa ttacgttgtc aggctggatt gtttttttga cagacatgct ataataactt
    24781 taattgaata tgaaggggag taccagatga acgaacgaat caaaaacaaa ttagctttac
    24841 ttcctgatca gcccggctgt tacttgatga aagataaaaa tggaacgatc atttatgtcg
    24901 gcaaagccaa aatattaaaa aatcgtgtcc gctcatattt tactggcagc cataatacga
    24961 aaacggaacg gcttgttagt gaaatcgaag actttgaata catcgttaca gaatcgaata
    25021 tcgaagcatt gctcttagag atcaatctga taaagaaaaa tgatcctaaa tacaacatta
    25081 tgttaaagga tgataaaacc tatcctttcc ttaagataac gaatgaaaaa tatccccgtt
    25141 tggtgattac tcgtaaagta ctaaaagaca aagcactgta ttttggacca tatcccgatg
    25201 tcggtgcggc gaatgagaca aagaagatcc tcgatcgatt atttcctctg agaaaatgca
    25261 aacctagtca gacgaaggag ccttgtcttt actatcattt gggacaatgt ctgtgtcctt
    25321 attatttcga cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg aaaaagtttt
    25381 taaacggagg acacgaaaaa atcgagtcag agatccatca gaaaatggaa aaagctgctg
    25441 aaaacatgga gtttgaaaaa gctgcagaat accgagacca gatccgtgcc attgagacaa
    25501 tcatgactcg ccaaaaaatg acaaatactg atttactgga tcgagatgta tttggttatg
    25561 cgattgacaa aggatggatg tgtgtccaag tgtttttcgt aagacaagga aagctaattg
    25621 aacgagatgt ttcaatgttt cctttctata acgaagcaga agaagatttc ttgacttacc
    25681 tcggtcagtt ttatcaggaa aatgagcatt tcattcctaa agaagtcttg attcctgata
    25741 cgatcgacaa gcccagtgta gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag
    25801 gagaaaagaa aaaattagtt gaattggcga ataagaatgc ctcagttgct ttgaatgaac
    25861 ggtttgatct gattgccaga aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa
    25921 acgcgatgaa tatcccgacc ccaatacgta tcgagtcttt tgataactct aatatcatgg
    25981 ggacgaatcc tgtggctgcg atggtcgtat tcattgatgg aaaaccggca agaaaagaat
    26041 atcggaaata taaaataaaa acagtcaacg gaccggatga ttatgcatca atgagagaag
    26101 tcatctatcg cagatattca cgagtgattc gcgaagggtt gcctttacct gatttgatct
    26161 tgatcgatgg aggaaaagga caagtagatg tggcaaaaga tgtcttagaa aatcaattag
    26221 gaatcgatat tcctattgct ggcatggcaa aaaacgataa acataaaaca aatgagttgc
    26281 tattcggtat agatttggct gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac
    26341 tccagcgaat ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg cagctacgca
    26401 gtaaaaacag ttttgcttcc cgattagatg aaattgaagg gttaggacca aaacgaaaaa
    26461 aaatgctgct taaagagttc aaatcgttga aaaatatcac agcagccacg acagaagaat
    26521 tacaagaaat cggactgccg aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg
    26581 aatgaggaaa gggcacttta caaagaaagt aaagtgtcct atcattttat gtacaacaaa
    26641 aagagcgttt acgttttgtt ggtaaataat aaatcgctta cgtctggtgt ctaaggatat
    26701 atgtgatttc ttgaagaagg agtgaagaaa tgttagaagt caaggatctt gttaaaactt
    26761 ttgggaacta cacagctgtc gatcatgtct ctttccaaat ccccgatgga aaaatcatgg
    26821 ggctaattgg ccaaaacggg gctgggaaga caacgacttt tcgtttgatc ttggattttt
    26881 tgacacaaga ccaaggagaa gtgttatgga atggccatca tcttggggag aaagattatg
    26941 atgtcatcgg ttatcttccg gaggaacggg gactttatcc gaaagtaacg attcaagacc
    27001 aactgcttta ttttgcagaa cttagaggga aatcaagaaa agagattgaa ccaaaaatcg
    27061 atttttggat ggaaaaattt caggtaaaag ggaaaaagac ggataaagta aaatctttgt
    27121 ctaaaggaaa tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa cctaaattga
    27181 tcattttgga tgagccattc agcgggttag atccggtaaa tgctgaattg ctaaaagatg
    27241 gaatattgga attgaaaaaa catggttcat gtgtcatttt ttctagccac aatatggata
    27301 atgtagaaaa aatctgcgat catttgatca tgttgcgaaa cggaaaaatg gttttgaatg
    27361 gaaaagtaca tgagattcgt gaatcatttg gccgtacgaa gttattcttg gaatctggtt
    27421 taagccaaca ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca
    27481 atacgttaga gattacctta gaagatcctg aagctgggaa agaaatcttt actcgtgcga
    27541 ctcagttcgg ctacattcct atgtttaacc agcaaccgcc aactttggaa gagattttca
    27601 aattgaaagc aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga tgtctattta
    27661 aaaaatgtta agtcgatttc tttcttgatc atgatattag tcccttttat catgctgggt
    27721 atcatttatg tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa aatcggcgta
    27781 ttggctgata atgagcaaat cagtcagcaa atgagccaag tcaaaacaga tgattttgag
    27841 ttcaaagcag ttgattctga agaaaaagca caaaaagagc tttcggaaga aaaaatcgat
    27901 gcgtatttag tagtctcaac tgagaatgat aagataaatg ggatgcttta tagcgaaaat
    27961 tcattaggac aaagtacaca actactgata caacaacaac tgactggctt gcaatcgatg
    28021 cttcgagcaa gtcaattagg catttcacca gaagaagtag ctagcttgag ccaacaagca
    28081 agtttgactc gacaaaaagt caattttgat tcaaaaggaa aaatgacagt tggagaagat
    28141 aactcgacag tccaatatgt cgtaagttat gtagcaacga ttgtcttgtt cattattatt
    28201 ttgacgtatg cgcagatcat tgcacaagag atcgcttctg aaaaaggcac gcggatcatg
    28261 gaagtgatcc tgtccagtac gactgcacaa aagcattttt acggaaaact aacaggtgtt
    28321 ttacttgttg cattaacgca aatggcatta tacggtatta tttttggtat aggatacagt
    28381 caattcaaag atatggaaat ggtcaaaaat atattagacg gtatctcatt ggataacttg
    28441 attggaccat tcttgtggta ttctcttcta ttcatgatct ttggcatttt gattttttct
    28501 gttttagcag ctttatgtgg ctctttggtc aacaaagcag aagatacagc aaaagcaatt
    28561 ttgccagtga cttatctatc tctaggcgga tatatgcttg gattgatact aggagcttca
    28621 gatccaaaca atatcgttat tcggatcact tcgtatattc cattcttatc ttcatatatt
    28681 atgccggttc gcttagcaaa tgagacagtc ggtatgggtg gagcattgat ttccttgttg
    28741 gtacttgtag ccgtaacatt cttattgatg atcttgtctg caaacatgta caaatcgaat
    28801 gtactcgtgt atagcgaagg cggattatgg agttcattga aacaatcgat ttcaattatg
    28861 agaaatgaac gaaaaaaagc ataaaatata gaaaaatata gaaggaagct ggacatttat
    28921 tgttcccagc ttcttttttg tatagaccta tttctatttt tttattatat tggatatata
    28981 tattcattga aaatagagca taacaacttt ataatgttga gtataccaat ttttggaggc
    29041 gttttaatgg accgctattt agatttactt tcagagaagt tccctacaac ggaagatgtc
    29101 atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt
    29161 ttgagcgata tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga
    29221 aatcttaaag aaaagatcaa agaccttttc tctgaacaaa tgacggaaac ggagatcagc
    29281 caattcacta tttttacggc ttatccagaa tatgtgttga cgactgattg gtacaaaaaa
    29341 caagataaat cccagctgat ccatcgtttg atcgatcttt tacggtttac gacagtcaaa
    29401 tacactcgat caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa
    29461 ctcttgtata tcgatgatcg tatcaacggg aaaaaagact atgtaaaaaa aatcattgaa
    29521 caacttgtct tgatgaagga agaagaaaaa tttttaacaa aacttgcgca gacgatccaa
    29581 aaatctgtca tcgatcattt gcatatcgtg ggtgatattt ttgatcgagg aactcaagca
    29641 gccaaagtaa tggatcgaat catggatttt tcctctgtag atatccagtg ggggaatcat
    29701 gatattttgt ggattggtgc gtattgcggc tctgaggcat gtttattgac gcttttacgg
    29761 attgccgcac gctacaacta tttatttgaa ttagagaaag aatatggatt aaatttacgt
    29821 ccattatttt cttttgccca tcaaacctat cagaagaatc cagtatttac ccctaaaaac
    29881 aggaaagatc tatctgaaag agagcaagaa gaattagaaa agatccatca agcactttct
    29941 atcctccaat tcaaatcaga acatcagttg ttgaatcgac gacctgaatt taaaatggac
    30001 gatcgtaaat tgttggaaaa aatcaactat gaggaatcaa cgatcgtttt agaaggtcag
    30061 ctctatgagt tgaaagggac ttgttttcaa acgatccagc cagatgatcc taaaaaattc
    30121 ttggaagagg aacaaaaagt gatcgatagt ttgatgtatt cattccagca ctcgttaaga
    30181 atgcaaaaac acatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag
    30241 catgtactgt tccatggctg tatacctgta gatgaaaagg ggcagctctt agcatttgaa
    30301 ttagaccaat cttatcgcgg aaaagaactg ctgggatttt ttgaaaaaca gataactgaa
    30361 agcgcaaaag atttgactgc aaatgaagat ttggcgaccg atttgatttg gtatgcgtgg
    30421 agcgggccat tttcgccttt attcggcaag agtaaaatgg caacttttga gcgctatttt
    30481 ctcacagagc ctcatacgca cgtcgaaaaa agcaatccct attatcactt gcgagatgaa
    30541 gagtggttca gtgaaaaaat tttggcagaa ttcgatgcaa aagaagaagg ttcagcaatc
    30601 atcaatggac acacgccagt aaaagtaaaa aaaggagagt caccgatcaa agcaaacgga
    30661 aaagcatttg ttattgacgg aggtctatcc aaagcttatc aaaaaacaac cgggattgct
    30721 ggctactcat tactatgcaa ttcttacggt ttccaaatcg tgacacatta tccgttcttg
    30781 cctattgaac aacaatttgc aaaaaaatct gatcaaacga atgtaaaaaa agtgatcgag
    30841 cagcagctcc cacgcaaact taatcggaat acgacaaaag gaatggaatt acagcaacag
    30901 attactcaac tccaaaggct gttggattat cgaaaggacg attgagaaaa atgctagcca
    30961 acattgctca atagactaaa aagcgactgc aacaaaaggc gtgtcgcagt ctccttcgga
    31021 attagctcaa aaaacggtaa aatattagaa gctatttttc tgtttatttc tctaatactc
    31081 aggtcagggc gctgatttca cgacctcttc cttaaacggt gttctcctgc caactcctcg
    31141 gtcacaagtc acaatatcca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    31201 ctcagagttg aacggcaggc tcacgaccta atcagaaaaa cgattaaaat tcattcacaa
    31261 agaaatcgaa taaacgctgt tcagatgaat ctacacgatg agaaagttcc ggatgccatt
    31321 ggacaccaag gattttgtgc atattatctt tactttggac tgcttcaatg agtccatcgt
    31381 tggaaaaggc gatggctttt aacaaaggag aaagatcttt gactgcctga tgatgatagg
    31441 aattcacaaa gtatgaatca gggagaaggt cacttaataa actgtccgaa acgattttga
    31501 cttcgtgagt tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg gaccattttg
    31561 gatataatga aagatcttga taaagtgttc cctccaacac gacattcaat agttgaagtc
    31621 ctcggcagac tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt
    31681 cgaattggtc tcgttgaata tttgtttccg tcagttttgg atgagggtct tccatgtaaa
    31741 gttgcggact aacatcctga ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt
    31801 gaggcgctga atctggagac atgactggca gaataagagg taatccttga gcatctaaaa
    31861 ctgcatccac aaatccctgc ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg
    31921 ctcgaatcag ttgattcccg gcaatgccga tgattggttt catttttatt ccccctaaag
    31981 tagactaatt gtaacaagga tttaaaaaaa tttaaactat gtaagacttg cccctatact
    32041 tatctgttct tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat
    32101 agttgaaaat attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc
    32161 gtaagcttca aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg
    32221 cctatactaa gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc
    32281 ttttttaaat cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc
    32341 agaagaagaa aatgagaagc gtgctcgaac cctacggcag cagttgaaga cctatgcaga
    32401 agaaaaagag caagaattgg agataatcca aacgaacagt cggcaacagc tgaaacagtt
    32461 ggtagaagaa aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa
    32521 agcagaagca gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc atcagagtca
    32581 agcaatcggt gcaatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa
    32641 aagtgaccct catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa
    32701 tccaaaatgt tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtagaaa
    32761 aatattttcc agataccacg attattgata aagaagcaaa attaagcgct ttaggcaatc
    32821 ggctgaaaga catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa
    32881 agaaactcca tttgaaacgt caagaacttt ccttacaaga cttggaagca aattattccg
    32941 aagaaaattt catgaataaa ttagaagaag tccttgattt aaaagaaaag tgggaacagc
    33001 taactgaaaa acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc
    33061 tggatattgc tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga
    33121 gtgcagcaaa ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac gtttatatgg
    33181 aagaaataga ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag
    33241 ttgctgctat agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg
    33301 aacaaaatcc taaagaaaaa ttgcaagaaa ccaaacagca attatccatt gttcacgagc
    33361 agcaaaaaaa gataacgaca gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat
    33421 gggtagaaga agtcacactt gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc
    33481 atgccactta tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga
    33541 tgcatgtgtt aaacgaaact gtatcagaag aagatatata cgttagtttc gaaacaccaa
    33601 actcagaaga gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttatcgaac
    33661 cttttgaatt gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacgc
    33721 cttggatgac accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttacg
    33781 gattgctgat gcccattggg acaatactag ctcaaaaact atttgtttta ccccgcggga
    33841 tgaaacgttt cgtaaaattc tttgagattc tctcggtacc atcgatcatc tggggattga
    33901 tctacagttc atttttcgga caggcattac caaaagaact atttggtatc cgcttgcctt
    33961 tcccaatcct atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg
    34021 gtctgataca gatcttggtt ggattattcg tggcagcaaa ggaaaatatc aggaggaaag
    34081 attacttgag tgctgtcagt gatggttttg cctggcaagg gatcctgatt gggatcgtta
    34141 ttgcattgat tggtgtcatg ttgataaaaa atacagcatt tgtttatctt ggcgcggggc
    34201 tatcgatcat tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag
    34261 tcaagggtgc tgccaaagga gcgtataatc tttacggatt gactggttat atcggtgatt
    34321 tagtcagtta tacacgtttg atggcactag gtatttcagg cggaagtatt ggcgcggcat
    34381 tcaatatgct agttgctttc atgccgccag ccgctcgatt cagtgtaggg atccttttga
    34441 tcatcgcttt acacgcactg aatatgtttt tgacactgtt aagtgcctat gtccatggtg
    34501 cacgattgca gtatgtcgaa ttttttggaa aattttatac aggaggcgga cgtgcctttg
    34561 acccgctgaa aacggcggaa aaatacgtca acatcaatca taagaaaaaa aacaataaat
    34621 aattggagga tttttataca atgatggatt acttaattag tcaaaatggt ggaatggtat
    34681 ttgcagtatt agcgatggca acagcgacga ttttttcagg gattggttca gctaaaggtg
    34741 ttggaatgac aggtgaggca gcagcagcgt taacaacaag tcaacccgaa aaattcggac
    34801 aggctttgat tctgcaattg cttccaggta cgcaagggtt gtatggattc gttatcgcct
    34861 ttttgatttt tatcaactta ggaaatgata tgtcagttgt acaaggattg aactttctag
    34921 gcgcttctct gccgattgct tttacaggtt tgttttcagg tatcgcccaa gggaaagttg
    34981 cagcagcagg tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt
    35041 ttgctgcgat ggttgaaaca tatgcgattt taggtttcgt tatttccttc ctattagtat
    35101 tgaatgccta agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca
    35161 gatgaatgaa gcagcagagc aagaaagagc ggcgttagag caagaagagc gaatgaagat
    35221 cgatcaaaac tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga
    35281 aaaacaaatc gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga
    35341 agtacgacaa gaaaatttga atgcgaagca ggaattttta cgacgattgt ttgcagatgc
    35401 aatagctgaa atggagaatt gggatgaatc tgaacaaatc cagtttatca aaaattcatt
    35461 gtattcatta cctttgacag gcaaagtaac attcattgct ggagaaaaat cagcagctta
    35521 tctttctcag acattgctgg atgaatggaa caatgaactt ccctttatga tggttttaag
    35581 tgatgaaact gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa
    35641 ttttcttttc agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa
    35701 tcaattattc gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt
    35761 cgcggaagag agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat
    35821 tctgtcgaag cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac
    35881 ggatatgaat atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg
    35941 ttaaaggaat cagcaccgga gcctgaaatc gtttggatct atacgatgcg ctatactttc
    36001 cataatctga aggttttgac aaaagccgag atgacaaatc aaaatctgga ccatttgtat
    36061 atcgacgatg gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca
    36121 aatgaactcc ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag
    36181 tctaccattc ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga
    36241 cggcttgccg ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac
    36301 ttgaccaata tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg
    36361 acgacggtac tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat
    36421 aaagatatgg aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc
    36481 cctgtcatcc gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa agatgattat
    36541 ctaagttcat tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc
    36601 tttttgaacg caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga
    36661 agccatttcg aaccagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat
    36721 cggtgtagtt ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg
    36781 ttatggtaca accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta
    36841 tggcgtgatt tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta
    36901 caaggaacaa atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat
    36961 agggctggaa gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta
    37021 tgaaaaggag atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt
    37081 aatggctgag aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg
    37141 agttatcgga gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga
    37201 gacttctgga attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga
    37261 attaggacca gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt
    37321 tatggaaatc acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca
    37381 tgagaaaaaa tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga
    37441 tgttatcggg atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg
    37501 gataaaagga acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg
    37561 tgtcattgaa accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag
    37621 acgtagtcgg ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg
    37681 agtgattgat acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt
    37741 tggtgcaggg aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt
    37801 tgtctatgtg ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc
    37861 tgaattgatt gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa
    37921 tacatcaaac atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc
    37981 ggaatatttc cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg
    38041 ggcagaagca cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta
    38101 tccagcttat cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc
    38161 attaggatca gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg
    38221 aggggatatt tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg
    38281 tcttgattcc agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta
    38341 ttctctttat tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc
    38401 atccatggtc accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt
    38461 gcgtttggta gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa
    38521 atccattcgt gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc
    38581 aagagagaaa caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg
    38641 tgcgttgtcg ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga
    38701 aaggatcgga cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat
    38761 tggtgaaatc aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta
    38821 aagaatatcg tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg
    38881 gtgtcaaata tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac
    38941 aagtattgga agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta
    39001 tcaatttacg tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg
    39061 aagacatgat cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga
    39121 tcctgcctga aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt
    39181 atccagatga gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc
    39241 gtgggcaaaa attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc
    39301 agatcgcgcg acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg
    39361 ctattggaat cacttttgaa gaagctgaat ttttcatgga agattttcgt caaacgggcg
    39421 caatcgaccg ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg
    39481 ctactcctcg tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg
    39541 ttttggttat catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag
    39601 cacggcgaga agttccagga agaagaggat atcctgggta tctttacacg aacttagcta
    39661 ctttattcga acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga
    39721 ttctaacaat gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta
    39781 cagaaggaca gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg
    39841 atgtactccc gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag
    39901 aagaccatgc agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa
    39961 aagaattagc cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga
    40021 agtttgctga gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga
    40081 ttactgaaac acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa
    40141 aacgaatcaa agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg
    40201 cacgattgaa tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa
    40261 ctgccacaag agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca
    40321 tcctgttgat tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag
    40381 caatgtctga ttttgtattg gcaaatgctt cgatcacgga acctttcatc gaagaattat
    40441 ttgttttgcc ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa
    40501 aagtacctgt gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg
    40561 ggtatttgaa ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc
    40621 caaaactttt ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg
    40681 aaaagacacg tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa
    40741 cgatctatta tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga
    40801 tcaaagtaaa aaatatgggt accactgact aagtggtatc tttttttaaa agaggaaaac
    40861 gatgattaaa caaagcaaac aaataaagaa acgtttgccg gctgttcagc ttctcgcatt
    40921 aggatttttt ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa
    40981 tagtgggcat gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt
    41041 aacgggattg acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat
    41101 catgcttttg attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc
    41161 attgataaag aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct
    41221 agaagaagta tcaggtgtga tgaagctcat gctttacatc ctaaaattgg cctttgttat
    41281 ccaattgatt ggtacggttg ctttatcttt tgtatttgtg cctgaatttg gctggttgaa
    41341 aggtatctgg tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt
    41401 gttaggagac agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc
    41461 tttgatcatt tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca
    41521 gaaaaagaag atgacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt
    41581 aggcggattc gttgtgttct ttttcacaga gcgcaatggg gcaacgctag tggaagggaa
    41641 tatgtttcaa cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata
    41701 ttattcagtt gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat
    41761 gtacatcggc ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt
    41821 gttgctgatc cagatgcgtt cgatgttgaa aggacgtacg agagcggaag catttggccg
    41881 taccatccga caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt
    41941 gtgtgtagtc gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat
    42001 cgaatatatt gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt
    42061 gacgccagac ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg
    42121 agttggtgta ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa
    42181 atacaaatat ccagaagaaa gtgtcatgat tggataattt catcttctga cagaaaccta
    42241 gtgatttagt aaaataagaa aataaaataa tttttcttgc ttcttggata ctttaagtgc
    42301 aaaatgttcg tttatttttt gaggcactac ccgttgttga acctcgggta gtgcctcttt
    42361 tttattgaaa ggaatcaata agatttgatt cctttattta tcaataggtt ataattcata
    42421 tttttttatt ttttctttta cgatacttag tccagaagct aaaatttcaa ggttattaga
    42481 ggatagggaa attcgaacga atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg
    42541 ataaagtcgg ataccgttag caagaaagtc attctcaatt atcttagggt caatctcagg
    42601 atcaactgga agaattcgat agtaagggac acgattcgtt ggtttttcta ctttgaaaag
    42661 agaatcaaaa agctgattgg ctctttttgt aagacttaat ttagtttcta agatcctatt
    42721 tgcttcattt gaatggagga tttgagtgat gacttccaca tctaatccag aagttttcac
    42781 atttatatta aaaattgctt gttctatctt gctttggaca tgtactggaa ctacaagata
    42841 agcaactcgc aatccagaac aaataaattt tgtcattcca gctaagtaga ttgtttggtt
    42901 aggtaataaa ttttggaaag gaggaatgat cgtatgttga tagtaagttg ttaaaaatga
    42961 atggatatca tcttcaataa cccatagacc ttctttttca atcacttcgg ctaattgctc
    43021 tcgtcttttg tgagagattt gaaaccctat aggattatta caagaaggca taagaaagac
    43081 accatgaatc ttcttttttc tacattcctg aatcaatata tcaggtcgca ttccttgctc
    43141 gtcaaaatca attggaacga tctctaaatg atataactga gccaattcaa tgaaattagc
    43201 gtatgtatat cggtctgttg ctattctatc acctggagag aaaagtgcag aaagtgtgat
    43261 agccaatcca ttttgaacac cggaaacaat agatagagat tctaaggacg gaatgactcc
    43321 ttgagttttt aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg
    43381 gtaattaagc aaaccaacaa gatttgggtt attactagtt gattgaataa acgggacaac
    43441 gaatttatta cattcttcaa aagaactaac cagaccaaaa tcaatgatat gttcggttac
    43501 ttcattggta gagatggtga tggactcaaa gacattggga gaaataaatg tgccacttcc
    43561 aatattcgta tacagcaaac ctttctcaat tccatattta taagccttgc caacggtagt
    43621 aaaatttaaa tctaaataat cagctagctc tctttgggag ggtaactgag tgtttttctg
    43681 taatttattc tttaaaatat cttgttctaa ttgttgaatc aacgatcgat aataaggacg
    43741 cttcaatttt tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag aattaatagg
    43801 cattttctcc actccttaaa attgtatacc atacaatttt accattgatt gtattgattt
    43861 tagaagttaa tcttttgtga ggagggaatt gtctatgaaa attattaatt ttgaggaagt
    43921 agtagcttct tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta
    43981 tgaaagtggt aaaatcagcc aaaatcctcg attggtggaa attttacctg acggaaaagg
    44041 caaaaatatg tttgcgatga tgcctgctta tttgggggag aacagatatt ttggcacaaa
    44101 agtcattacc gcttttccgg ataatcatca gtatcattta ccttctcatt taggagagat
    44161 tttcttgttt gatgctcaaa acggactacc caaagcattg ataaacgcaa atgcagtcac
    44221 ctggattcga acagctgctg tatctgcgct ggcaacagat tacttagcaa atccgcatgc
    44281 agaaacactt gctttaattg gagctggtca acaagcattg tcgcatttga ctgcgatttc
    44341 taccattcgt cccatcacta cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac
    44401 ttttatcgct aaagctaaaa aacagtatcc taaaataacg tttataaatg caatatcagt
    44461 agaagatgca gtacgtccag cggaaatcat ttgtacgttg acctctagta aagaggcatt
    44521 tcttcaaaaa gagtgggtaa gggaagacgc acatatcaat gctgtgggca catttactcc
    44581 tgatacaaga gagataagta catcgttgat ggaaacttct caaatctatg tagatgatta
    44641 ctcagctgcc ttaacagaaa gcggggacct gcttatagcc atggcagaag gagatttaga
    44701 ggaatcttgt attgttggta cattaggcga attagtttct gagaaaaaaa cattttataa
    44761 aggagaatcg aggccgacga ttttcgacgc agttggtcta gctattgaag atctttgttg
    44821 tgcagaatat atttataaca aattagagaa aggggcttta aaatgaactt agattattac
    44881 gtagttgatg catttgcaga tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta
    44941 gaagaatggc tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa
    45001 acagcattta cagtgaagaa aaaccaagaa tttgagctta gatggtttac gccagatcga
    45061 gaaatcgatc tttgtggtca cgcgacatta gcaacagcat ttgttttatt taactattac
    45121 aaaatacctg atgaaacgat taaattttct actcaaagcg gtaatctatt tgtgacaaga
    45181 attaaagatt attattacat ggattttcct agtattatgc cgaaaaaagt gccgatattg
    45241 tctgaatatg aagaagcgat tggggctaag attaaagaag cctacttagc acgtgattta
    45301 tttttcgtat tagaagatga aaaaacggtg gctaaattgc agccagattt tacagctatc
    45361 aaaaattttg aattaggaat aggagtcatc gtgacagcag aaagtgtgca aaaagacttt
    45421 gtttctagaa ccttctttcc aaaattaaca atcaatgaag atccagtatg tggatcagca
    45481 cattctaatc ttattcctta ttgggcagaa agaatgaata aaacaaaatt gtcagcttat
    45541 caaatgtcat caaggggcgg ggatctagaa tgtgaattaa aagaagatcg tgtgataata
    45601 ggaggaaaag cagttttgtt ttctaaaggt acctcgttta tttgatttct ttcaaatgaa
    45661 ttaaaaataa attggtggag agtggcaacc ttatagctac aatggttgtt aaaaaaagac
    45721 gcaaactttt tcaatttaat gaaaagtttg cgtctcattt taattattgg tgtttgtttt
    45781 ttattcaatc aagagtaaaa tgccccatac taactatcct cggatgactt cgattttata
    45841 accatcaggg tcaatgacaa agtagtaaga aggcgcagtg ccaggtaatc cttttaagtc
    45901 agtaatctca agaccggcag ctttatgttg ctcgtgtaat ttttctagat catcagctgc
    45961 tatagcgata tgaccatagc cgtctcctag atcatacgca ccatgatcat agttgtaagt
    46021 caactctaat tcatagtcat ccccaggaag tgtcaaataa acaagtgtaa atttgttttc
    46081 tggaaaatca cggcggcggc tttcttcaaa accaaatgcc ttttgataaa attcgattga
    46141 tgcttcaaga tctttcactc gaacacatgt gtgagccatt ttcatagatt catctctcct
    46201 tttcctttta ctcaatcata acaaaaagaa gtaaggaaat agttgatttg tgcttgttca
    46261 tgagaaattt ctcatgtaaa atggatagaa aataaaactg ttaaggtgga aactattcat
    46321 ggataaacca atttttggtg aaaaaaagca atttgctgat tatcaaaccc gttatgcagc
    46381 atatattgtc atagaaagag atcagcagat tgcagtaatc gaggctccaa atggtgcttt
    46441 ttttcttcct ggcggggaaa ttgaaggaga agagacgaaa gaagaagcga ttgtccgtga
    46501 gctgatagaa gaaatgggaa ttgtggcaga aatttcttct tatctgggag aagctgatga
    46561 gtatttttac tcgaattatc gtgcaactta ttattataat ccaggatatt tctttgttgc
    46621 tgattcttgg aagcgtatag gagaaccaac tgaaaagacc aataaaattt ggtgggtgag
    46681 ccctcaagaa gcgcttgtga aattgaaacg tggtagccac cgatgggcag tcgagagatg
    46741 gatcgaaaaa aataaaaact gaaagaaagc gagtagatca gctttctctc agttttttta
    46801 tttattacag tacatatttt ttgattgctt cagctacacc gtgctcgtcg tttgatgaag
    46861 tgatgacatt tgctgcttct ttgacaagag aaacggcatt tcccattgct actcctagac
    46921 cagcatattc aatcatggat aagtcgttct cgttatctcc gatagccatg atttcatctt
    46981 gatcaatacc aaggtgctgt gcgagattcg caactgccgc acctttactt gcttctttat
    47041 ttagtacttc gaagtaatag ggcgcacttt tgaccgttgt gtatttatca cggaaagttt
    47101 gcggtaaacg cgcaattgct gcatctaaaa tttctggttc gtcgatcatc atcattttga
    47161 cgatgttcat gtctggtgtc atttcttcag gtgttcgata ttttagcggc atcttgacta
    47221 aacttgcttc gtggatcgta taagcgctga tatctcgatt aggcgtgtag atcgctttgt
    47281 catcgatcga atggagatga ctgccgacac ggcgtgccat cacttcgatc tctaggaaat
    47341 cttcatgtgt cagtccgtga cgggcgatga ttttacctga tttcgtttgt tgtacgagtg
    47401 ccccgttata agtaataacg taatcattat cttgaaaaag gttcaactcg tttaactgtt
    47461 cctggacacc tggtaaggga cggccagtgc ataaaacgat cgaaacacct tttgcaacag
    47521 cagcggcaat cgcttctttt acttcttttg ttacttctct tttctcattt aataaagtac
    47581 catcgatgtc gatagcgact agtttgatag acataatttc ctccaatata ttttaattta
    47641 tttcaatcaa tgcgccatta cggatatggc tggcaaattc ttgataagtt tcgtcaaata
    47701 aatcataatg atcacgcaag gacggatcaa ccatttcttt tggaaagaaa aatcgctcat
    47761 ctccttgttc ttgtcccgct aatgcagcaa caagctgact aacctgtgat aattctatca
    47821 aagttccgtc atttcgcaaa atctcgattt gtgtgcgatg tctgccttgt tctgggcgat
    47881 aaaaatcata aggtagatcg tagctagaat tcacagcagt gtaataaaca ggattatatc
    47941 cgaccttttc tactaacaaa gtgagttctt ggactaattt tgaatcccgg ttgccagaaa
    48001 aagtagcaga tttcagtggt ttacgattca aaaaacgttt agctaaatca tttaaaatag
    48061 gatcagcaga ttcagaccat tgagtaaagt aagtgcccaa gaccccatca tcaagtttga
    48121 ggtattcttc taaagtaaag cttccgtcaa gaaatggcag aagtaattga gaatggagtt
    48181 caaaactgct ggcatcttct tgatacaact cgtgtgcgcg atgaagcaga tgctccaaaa
    48241 ttacttccat accacgggaa acaggatgga agtacacttg cacatacatt tgatatcggc
    48301 taacgatata atcttccact gcatgcatgc cactcatgga aaaagcgatc ccgccttcat
    48361 aaggacgaat cacacgtaat attcgggtaa gatcaaatgt tccatattct gttccagtga
    48421 aataagcatc ccgaagcaaa tagtccattc gatctgcatc gatctgacta gaaatcattt
    48481 ggacaacttg tgggttgggg taagtttttt ggatcacact agctaccttt tcaggaaaac
    48541 cttcttctac tcggttaaga atctgataca cttctgtttc tggagaagtg atgatctcta
    48601 cagtgatggc ttcatgattc gtatggaaga tatgttcaaa tgtatgagag taaggaccat
    48661 gaccgacatc atgcaacagt gccgcacata aagtcaccag ccgttcctga tcatcccagc
    48721 ctccgttgcc tattttttct tttgaaaaat ttcgagaaaa gatatcgcag attcgtcttg
    48781 aaatctcata aacgcctaat gaatgagtaa aacggctatg ttctgctccg tgaaaagtga
    48841 aagaagaagt gcctaattgt ttaatgcgtc gtagacgttg gacttctttc gaattgatca
    48901 aatctaagat tacttgatgc tggacatgga tatagttatg gactggatca cgaaaaactt
    48961 tttccatagg cagcatttga tatttataag gggttgtcat aatgtagctc ctttatgtta
    49021 ttacgttgtt ccattctctg gatttggttg ttccattgct ccagctggat ttcttttttt
    49081 agccagtcgg tagaatcctg taaatcactt gcagaataca gcttttcaaa agcttttgca
    49141 aatgcttctt tgacttcttt tacaaaaagg ggttggtcaa gcagttcttc aagtgttgcc
    49201 attgtagcag gatccactgc aggatagcca tttgtaccaa agtcttcttt taaagcagat
    49261 tgataaaatc gcttcatgac ttgcccgcgt ttttcctgat taccgttgac acttaagtac
    49321 atcataacag aaatcccatt tttgatccga cgctgagcga taccggcaaa ttttttgcca
    49381 tggatgctta aatcataagt tccaggacaa taagaatggg tgatttcgaa tgcttgtatc
    49441 ggtgcctttg gaaaagcgta tttagtcaat aggagcattt gctcgtaagc gtcatctatg
    49501 gttatttttt ttgtgtcact ttgggggaaa atcaaagaaa tattcaaaat tccttcatct
    49561 gatacgatcc ctaatccacc tgaatttctg atcactggag cgtaacctgc atctttcagt
    49621 gtttggatcc cgtcacttag aaaaggagtt cgaacatcct tcatacctaa gatgactgtg
    49681 cagtcatatt gccagaaatg taaaaagatt tgctggtgtt ctttagaata ttccgttaat
    49741 atatctgtta atgcaaaagg ggaaaagtcg tttttttgat aaatgtgctg atctagtcga
    49801 atcgcagggt tcattcaacc ggctcctttg tataaattca tattttctac aatattatac
    49861 tctaaaatca aagagttgtt ttggaaaaaa ggttttctgt aaaaatggac aagttggttg
    49921 acaaaacaaa agaaactagg aacaatgtag ctatgagaag taaaggagaa tgaataatgg
    49981 aattatcaca aggaacaccg atttcaatca aattgcgtac aaaagtaaaa cagcaagggg
    50041 aagtgcaaga tttttatttt gatttgaaag ggcaaatggt gacaattggc gatacgctgt
    50101 acatacgtta taaagagatt caggaggaga cgggcgatga gattcctgta acgatcaaat
    50161 tagttccaga tggtcatgta cagctgatcc gtgcaggaga aatgcggatg cgtttgaaat
    50221 ttggctataa agaacggtta gaaacatcct atcgaacacc atacggtatg atccagattg
    50281 ctacttttac gaaagaactg catgttagtt tgaaagaccg tcccacagga gggaaggttc
    50341 gaatcgatta tgatctgttc atgggaccgg aaaaaatcgg cgaatattat ttgaccttgg
    50401 attttacagc gtaaatataa aagaaaagtt tgattttttg tgtctgattt tgtatgataa
    50461 ggagtagact gtgaaaggac gtgtcctgat tggaaattag tgtatttgaa ggtgcaaaca
    50521 aaagcgaatt atcaatgatc gaagtagcac acgcgatctt agaacaacgt ggcgatgtca
    50581 tggatttttc tgatttagct aaccagatcc aaaactatct tgggaaatca gacagtgaaa
    50641 ttcgtgattc actcgcacaa ttttatactg acttgaatat tgacggcagc tttatctcat
    50701 taggggataa tcgttggggc ttgcgttcat ggtatgctat cgattcgatc gatgaagaag
    50761 taaaccatgg aatggacgaa gaggatgaag atactccacg tcgccgtaaa cgcaagaaag
    50821 tcaacgcctt tatcaatgat gatgaagacg cgatcgatta caatgacgat gacccagaag
    50881 atacagatct gacagaagaa gatgacgatg atctgtttga tgacgatgat gatgaagatg
    50941 aagaaatcgc tgcttataat tctgacttgc aagaaatcgg agcagatgac ccgacagatg
    51001 atgaagaaga cttgccagga aatatcgaag aagatttaag tatcatagac gaagacgatg
    51061 atgatgaaga ttatgaagag gaagaatttt ctgactctga aaaagaataa acattatttt
    51121 cttacgagag gttgtgacag agacgatcag cctatcagaa taaggaggtg tgacacgat
//
LOCUS       DACDTH010000012        48724 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDTH010000012 DACDTH010000000
VERSION     DACDTH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.012"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1315)
                     /locus_tag="IWK54_000780"
     CDS             complement(<1..1315)
                     /locus_tag="IWK54_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9592923.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYK"
     gene            complement(1317..1985)
                     /locus_tag="IWK54_000781"
     CDS             complement(1317..1985)
                     /locus_tag="IWK54_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9592924.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2115..2699)
                     /locus_tag="IWK54_000782"
     CDS             complement(2115..2699)
                     /locus_tag="IWK54_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9592925.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3030..3263)
                     /locus_tag="IWK54_000783"
     CDS             complement(3030..3263)
                     /locus_tag="IWK54_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP9592926.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3278..4228)
                     /locus_tag="IWK54_000784"
     CDS             complement(3278..4228)
                     /locus_tag="IWK54_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9592927.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4225..5067)
                     /locus_tag="IWK54_000785"
     CDS             complement(4225..5067)
                     /locus_tag="IWK54_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9592928.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5069..5788)
                     /locus_tag="IWK54_000786"
     CDS             complement(5069..5788)
                     /locus_tag="IWK54_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9592929.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6480..6638
                     /locus_tag="IWK54_000787"
     CDS             6480..6638
                     /locus_tag="IWK54_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9592930.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            6710..7936
                     /locus_tag="IWK54_000788"
     CDS             6710..7936
                     /locus_tag="IWK54_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9592931.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(8024..8416)
                     /gene="rpsI"
                     /locus_tag="IWK54_000789"
     CDS             complement(8024..8416)
                     /gene="rpsI"
                     /locus_tag="IWK54_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9592932.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8432..8875)
                     /gene="rplM"
                     /locus_tag="IWK54_000790"
     CDS             complement(8432..8875)
                     /gene="rplM"
                     /locus_tag="IWK54_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9592933.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(9394..9861)
                     /locus_tag="IWK54_000791"
     CDS             complement(9394..9861)
                     /locus_tag="IWK54_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9592934.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(9981..11519)
                     /locus_tag="IWK54_000792"
     CDS             complement(9981..11519)
                     /locus_tag="IWK54_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9592935.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11599..12285
                     /locus_tag="IWK54_000793"
     CDS             11599..12285
                     /locus_tag="IWK54_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9592936.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(12486..13013)
                     /gene="msrA"
                     /locus_tag="IWK54_000794"
     CDS             complement(12486..13013)
                     /gene="msrA"
                     /locus_tag="IWK54_000794"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9592937.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(13097..13774)
                     /gene="pgmB"
                     /locus_tag="IWK54_000795"
     CDS             complement(13097..13774)
                     /gene="pgmB"
                     /locus_tag="IWK54_000795"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9592938.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13771..14901)
                     /locus_tag="IWK54_000796"
     CDS             complement(13771..14901)
                     /locus_tag="IWK54_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9592939.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(14901..17084)
                     /locus_tag="IWK54_000797"
     CDS             complement(14901..17084)
                     /locus_tag="IWK54_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9592940.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17081..18106)
                     /locus_tag="IWK54_000798"
     CDS             complement(17081..18106)
                     /locus_tag="IWK54_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9592941.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(18151..18978)
                     /locus_tag="IWK54_000799"
     CDS             complement(18151..18978)
                     /locus_tag="IWK54_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9592942.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(18975..20042)
                     /locus_tag="IWK54_000800"
     CDS             complement(18975..20042)
                     /locus_tag="IWK54_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9592943.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20068..20904)
                     /locus_tag="IWK54_000801"
     CDS             complement(20068..20904)
                     /locus_tag="IWK54_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9592944.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20901..21770)
                     /locus_tag="IWK54_000802"
     CDS             complement(20901..21770)
                     /locus_tag="IWK54_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9592945.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(21840..23123)
                     /locus_tag="IWK54_000803"
     CDS             complement(21840..23123)
                     /locus_tag="IWK54_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9592946.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(23137..24822)
                     /locus_tag="IWK54_000804"
     CDS             complement(23137..24822)
                     /locus_tag="IWK54_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9592947.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            25097..26122
                     /locus_tag="IWK54_000805"
     CDS             25097..26122
                     /locus_tag="IWK54_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9592948.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(26717..30370)
                     /gene="rpoC"
                     /locus_tag="IWK54_000806"
     CDS             complement(26717..30370)
                     /gene="rpoC"
                     /locus_tag="IWK54_000806"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9592949.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(30449..34066)
                     /gene="rpoB"
                     /locus_tag="IWK54_000807"
     CDS             complement(30449..34066)
                     /gene="rpoB"
                     /locus_tag="IWK54_000807"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9592950.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLPQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            34794..35762
                     /locus_tag="IWK54_000808"
     CDS             34794..35762
                     /locus_tag="IWK54_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9592951.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            35780..36685
                     /locus_tag="IWK54_000809"
     CDS             35780..36685
                     /locus_tag="IWK54_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9592952.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            36682..37488
                     /locus_tag="IWK54_000810"
     CDS             36682..37488
                     /locus_tag="IWK54_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9592953.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            37645..38613
                     /locus_tag="IWK54_000811"
     CDS             37645..38613
                     /locus_tag="IWK54_000811"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9592954.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(38930..39454)
                     /locus_tag="IWK54_000812"
     CDS             complement(38930..39454)
                     /locus_tag="IWK54_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9592955.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            39741..41552
                     /gene="scm"
                     /locus_tag="IWK54_000813"
     CDS             39741..41552
                     /gene="scm"
                     /locus_tag="IWK54_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9592956.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(41707..42693)
                     /locus_tag="IWK54_000814"
     CDS             complement(41707..42693)
                     /locus_tag="IWK54_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9592957.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(42716..43282)
                     /locus_tag="IWK54_000815"
     CDS             complement(42716..43282)
                     /locus_tag="IWK54_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9592958.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(43296..43712)
                     /locus_tag="IWK54_000816"
     CDS             complement(43296..43712)
                     /locus_tag="IWK54_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9592959.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(44169..46103)
                     /locus_tag="IWK54_000817"
     CDS             complement(44169..46103)
                     /locus_tag="IWK54_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9592960.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            46405..47340
                     /locus_tag="IWK54_000818"
     CDS             46405..47340
                     /locus_tag="IWK54_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9592961.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            47430..48533
                     /locus_tag="IWK54_000819"
     CDS             47430..48533
                     /locus_tag="IWK54_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9592962.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            48546..>48724
                     /locus_tag="IWK54_000820"
     CDS             48546..>48724
                     /locus_tag="IWK54_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9592963.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
BASE COUNT        14513 a        10085 c         8758 g        15368 t
ORIGIN      
        1 ttttataaag aaattccgga tcatgagtag ctataactac tgcacccttt tcttttaagt
       61 ctttaataag gtcagcaatc ctttccatat taatataatc caacccactt gtaggttcat
      121 caaatagaaa taaattagtt tttgacaatg aagctataga aaccaataat ctttgttttt
      181 ctccaccgga taaatcaaac ggtaattgat ctttcatttt ataaagttcc atttttttta
      241 aaattttttc ttgctgatct ttactaacta tttcattccc cagggctaac tcgctcctca
      301 ctgattctgt gaaaaattgg taatccattt cttgcattac taaaaacggc gatgacaaat
      361 tttttttatt ttcgattatc tttcctttgg tagggcgaat aatcttcatg agtattctta
      421 gtaaggttgt tttccccaca ccgtttttcc ctaccaaacc aataacatct ccaactttga
      481 catcaaaatt tatgtctttt agaatatttt cataagtaat ttcttcaact tttaatacat
      541 ctgcacttac cttttcgata tagggaactt caatcgaact attaaaaatg tcaaatttcc
      601 ttaatgcgtt attgttttca gtttttaaca actgaatagt tgtagttttt acagttctat
      661 ttttaacaaa tagaacttga tcaattaatt ctttcaaata gtaaaaacga tgttcagcta
      721 taacaatagt aaatccttta ttttttaaat cacaaagtaa gtcagcaagt ttcttagtcg
      781 ctttgaagtc caaatttgaa gacggttcat ccaatataat taatttagag tttagcgata
      841 aactacatcc aatagaaatt ttctgccgtt caccacttga tagcgaaaaa atgtttttat
      901 ctaatatatt tgaaagatct aataattcag ctaattcatt gattctttta tccatatgct
      961 cttttgtaaa tccaaagttt tccatcgaaa atactaactc actcgttgta tttgttgtaa
     1021 agaattgtcc tctaggatct tgacttacgt agccaatatt ttttgatata ctttcttgtg
     1081 ttaaggattg tagcgagtta ttttcaaata aaatttcccc aaccaatttt ccgtcataaa
     1141 gtcttggaat aagtccattc attaacttta atagtgtgga tttcccactc ccagaagaac
     1201 cagtaatcaa tgtaagactg ccttttttta ttgaaaaatt aaggtcaaca agcgcttgtt
     1261 ccttaacatc cttatatgaa aaagaaacat tgttaaattc aaacaattct ttcacctcaa
     1321 atcaaaatac ttaagaaaaa aatagtaacg gaaaacagga tgatatagtc taataagctc
     1381 atgtttgttt tatagtaaga acttcttttc acaggagatt ctatcccctt tgttattgca
     1441 ttagcggtca tttcttcagc aatcatagtt gcacgtataa gaacgggaac taacagaaat
     1501 tccaaatatt gcagaggctg aaaaaaagac aagcgaatgt ttctcatttt taaagattct
     1561 ttaatcattc ttatttcaga acgataagtc ggaaagaatc tgatcactac tgtaatactt
     1621 aacattattt ttttcggtaa atgtattttt tctaaagagg ataataattc attgctagta
     1681 tagacactaa ccaacaacca tgagatcatt acgaccggca acattcgaaa tacaatatat
     1741 aaggatgtcg caaaaaatat agtaaaaaac tctatcttga tagcatcaat aattttcatt
     1801 actaaaaaca gaactgaata aatcaagatg aaataaatac atgtctttat tttacgaaac
     1861 atcaatacta aaactgtagc aataatgaaa caccccactt gaaatatatt tccctttcct
     1921 aaaaaaataa tgaccggaaa aaaaatattt aaagtaatca aggttaacgg attgattttt
     1981 gacactcttt cacctccaaa aaataagatc gcactgttat cttaagctga taaaaatgca
     2041 ccgttattca gataacagtg cgatcttatc aaaaagcata actgagaaca attctcaatg
     2101 caagcttttt ttaattaact cagtaattca atccacccag gaacagaaaa ttgatacaat
     2161 cttctggtta atagcatagt cttttctttt gaatagctat gcttcaatat atcatagaat
     2221 gtagcgtaga atgagtaaat gtagaagtgg atttcttctt cattaaagac taaattttga
     2281 ttgatcattc cgatagtctt taacttatct agacaacaga ctgtcttttt cgtcacaaat
     2341 gatgtaattt tttcaacaat atcttgatat ttggttccag ctgattggaa taaaatcaaa
     2401 tcgaatatac gtttattctc aaaaatataa ttaataaatg gatctaattc attgaagttc
     2461 tctctccaac ttttctctat atctatattt tctaagttta agaggtattt ggtaaactct
     2521 tcattataca tattaataag cgcattaata tgcggctgaa taatttcttc aaataacgct
     2581 tccttagaag taaaatgttt ataaaaagca cccgtagtta cgcctgcggt caggcacagt
     2641 tgccttatct tagtttgctg aaatccttct ttttcaaaat ggcttagtcc actttctatt
     2701 aacttttggt gagttataat ataatctctg gtttctatgg gtacttcctc ctaataagat
     2761 gaattaactt tattatctta cacaggactt gaagtttcaa tcgttaacgt ttattactga
     2821 actcattcct tagagctaaa ctaatgagaa agagcagaat aactgataca aaaagcaatc
     2881 ctacaagaat taatagactc atctaaatca ttcacctttt ctaaaataat ccgagaaaga
     2941 tttaaaaaaa ttatatccag ctattaggat aagtcctatc ataatcactc caatagccat
     3001 tttcttatcc tccctaaaaa taaagtcttt tacttaaaac cttttttgta atcgtcaata
     3061 aattttttcc ctttatcagt taattgaaat cctatatcta aatagtcctc atatctaagt
     3121 ttagccagca tctcacttac agatgctgga ctaacactta gaacttttgc taaatcatta
     3181 ttttttaatt tcttttttct ttttgataat tcctgaattg ctagtaaata aattagtata
     3241 gtagtcgttt taatttgtac catacctacc ctccaaatta tttcgaaagg ccttcagcaa
     3301 ttttatccag gttccatttc atcatcgcat agtaactatc accatcttgt cctttttctg
     3361 caattgaatc agtaaaaatc gttgaataga taggaatatt ggtatctttt gatactgttt
     3421 tcattggtct atcatccaca ctgctttcta cgaataagga gggaactttt gttgtgcgta
     3481 atttttcaac taagtgtttt atttgatctg gtgttccttc ttcttcagtg ttgatttccc
     3541 aaatgtaagc agaaggcaca ttatacgctt tcgagaaata tttaaagcat ccttcacttg
     3601 taacaatcat ttttttatca ttcggaattg aagcaaattt agatttagct tctttgtcta
     3661 gagaatccaa tttttcaata tacttatcta gattttcttt atagaatttt ttattatcag
     3721 gatctttttc cgctaactgt ttttcaatat ttttagcgta aataataccg ttttctaaat
     3781 ttaaccaagc atgaggatct tccttccctt tctcactctg accctctaag taaataacat
     3841 ctatgccatc acttgctgca aaatagtctt tgttttcctc tttgttcgca tttttaacta
     3901 attttgtaaa ccaagcattt cctccagttt ccaagttaac accgttataa aaaatcaaat
     3961 ctgcttttga agttttttga acatcttcag gcaaaggttc atattcgtgg ggatctttcc
     4021 caataggtac gatactgtgt aaatcaattt tatcttttgc tatattttcg gtaatatctg
     4081 ctaaaatcga attagtaact actactttta atttttcgtt cgaagccact ttatctttgt
     4141 ctttactatt tattgaacat gccccgaata taaaaagagc aatcaaaatc cccagacttc
     4201 ccagtactgc tttccatttt ctcatcataa cgatctctcc ttctttctta aaaatttttg
     4261 ttttggtgag attagaaaac taattacaaa taagaatgcc gctgttaaaa caatacttga
     4321 tcctgctgct atgttaaaac tataaccaat aaacaatcca agcaatgatg aacttgctcc
     4381 gactgttgca gaaataaata tcatagtttt caagctattt gcatataaat atgctgtagc
     4441 tgctggtgta attaacattg caacaattaa tatagttccc acactttgca tagctgttac
     4501 tgctactaaa gttaatagaa ccatcaatag ataatgataa aaactgacat tcatccctat
     4561 tgcttttgcc attaatggat caaaagaagt gattaataat tctttaaaaa atagaactac
     4621 tataaccaac actaaaaccg aaacgccaat tgtaatccac ttatcaatat cttgtacagc
     4681 taaaatattt ccaaataaaa tatgaaataa atccgttgag ctatttgcaa ctcctataag
     4741 gataactcct agtgctaaaa aagaactgaa agtaatacca attgctgtat ctccttttat
     4801 tacactattg ctttttataa aagtaataat gatagaagct aatagaccaa atacaatcgc
     4861 accaataaaa aaattaatac ctaaaatata agaaattgct actcctggca aaacagcatg
     4921 agaaatagca tcacccataa gagacattcc tcttaaaata ataaaacaac ctactgttcc
     4981 agaaacaatc cctataacta cagatgtaat tagagcattc tgtaaaaagt gaaagtcata
     5041 cagaccatta ataaaatttt gaagcatatt attccccctc tccaataaat atagtatctc
     5101 cataggcttt ttttaaattt tcttcattaa atacagattc tacacttccg tgagcaatta
     5161 gatttcgatt caaaagaata atattatcaa aatacttctt taccttgctt aaatcatgat
     5221 gcacaattag cactgttttt ccctgatgtt tcaatttttt taacgtatcc ataattattc
     5281 gttcactaac taagtctatc cctacaaatg gttcatctaa aaatatgaaa tccgcatttt
     5341 gagcaagaca tcttgctaaa agtacacgtt gaaattgtcc tccagataat tctcctattt
     5401 gatgattaga atattttccc atatcgacct tttccaaagc ctttgaaact ctttgccatt
     5461 ctgcattttt aattctttga aaaactttca ttccggcata tgttcccata gatacacatt
     5521 cttttacttt tattggaaaa gtaaaatcaa tatcagcttt ttgctctaca tatgcaatct
     5581 tcaccagttt tttattgata tcttcattat taatggagac ctctccctga tgagggataa
     5641 tatttaacat tgctttaagc aatgtggatt ttccagcacc atttggacca ataatacctg
     5701 ttaaagcacc ttcttgtatt tttaaagaaa tctcgttcaa tgctaaaaaa tcgttataac
     5761 aaacattaag ctttttcact tctaacataa ctttctcctt ctaaatgata atgattatca
     5821 cgatcatatg gtatacaaaa attatatttt agtcaaccct aaattttata gtttagattt
     5881 ttttctgttg ctgaagatta gaatgacaaa actatttgaa actacgcgta tagtaagtag
     5941 ctttcaaaat atataaaaaa ggagcgttct caaatgagaa catgctccta aaactacttt
     6001 attggcgtat ggaaaaatta tacttgaagt ggatagcggg ttgaataagt tgataagcat
     6061 caaaacattt ataaaaatta ttcaagacta tgtctgttcc atatacaaat atgaaaatca
     6121 gtaaactcca tttaactttt ttcgtagtgt gtctttttat acatgtttat cgttattaat
     6181 agtgaatagg aaaaaaacta tttttgatca ttgacaatta aatacaattt ttgaataacg
     6241 atccctgcta ttcgagctag aaactcaagt acgccatgac ctgattagcg acaaatactt
     6301 agagatttct ttttgttctg ggaaaacaaa atgccatgac aatagcagca aataatgata
     6361 cttccgtaag gctcgccgta aactggggag aggtgaaatt cctatgtgct tacccaaagc
     6421 aattcaaaaa tttcataact actattttag gagatgattt tatagaatcc agagtctacc
     6481 tgaataaaaa acaaacctgt gcatttctag gaatcgataa taatactcta gataagtgga
     6541 ttcgtatata taatttccca gtaataagag ttggtggtgt agttcgcttt gacgtagaag
     6601 atattaaaga ctttatgaat acatttaaaa taacttgaaa ttttcttctc gccgcatata
     6661 ctaaaattag tgaaatatac tgccttactt tttttaagga gtgaggattt tgcctacaaa
     6721 gctttcaaat gggaaatata aaactaatct gagatatcca aaaaggttta aagaaataac
     6781 aggaattgca tcagaaaagt atcaaaaaac atttccaaat aggcaattgg cgattaaagc
     6841 cgaaaatgat atgaagaaga aaatagaaaa agttttgaga gaggagaatg caaactcatt
     6901 agaattaaaa ggaaaaataa cgtttaaaaa attttacgaa tctaaatggc ttcctagata
     6961 tgaattaggt caaacaatta gatcaaacag gcctcctagt gatataacca ttagtaatac
     7021 aaaagatata tttcggttgc atattttacc tatgtttggt gaatacgcaa tgaattatct
     7081 aaatatcaat actgagataa tatcagatga attaaccaaa aaatcgaaag aatatgcaaa
     7141 tataaaaatc ataaaaggat atgttcgttc catgttcgat attgcagaaa tactaaatta
     7201 tatcgaattt aatcgaacta ctaaaattat tcaaagtatc actgcaccta aaaaaaatgc
     7261 tttagaagaa aaaagaatac aagaaggtaa gcaagcacta tcatcaaaag aacttactaa
     7321 ttggatagaa gcagtaaatg atgattttaa taatcattta cttacttttc atgattacac
     7381 actttttatg attaccctgt atcttggtga tagaaaaagt gagacttacg ctttgcaatg
     7441 gaaatatatt gattttgaaa aacaaacggt ccgtcttaaa catactttag ataaatatca
     7501 aagaaaaaag tttacaaaag gtcgaaaaga taccgtcatt caagtaccag aagttgtaat
     7561 gacattgcta agtgaatgga aatcggtaca agctgatcaa ttgttgaaat taaaaattaa
     7621 gcaaacgtta gatcagtatc tctttactta tactaaacca tctggagaag taaattgtcc
     7681 tgttcatgcc gattacctaa actatcgaat caattcaatt aaaagaaggc atcctgactt
     7741 agttcattta tctccacaca aactccgtca tacttatgca actatagcta gacaaggtgg
     7801 agcagatatg aatcaaattt caaatgcttt aacacactca gatatttcta caacaaagat
     7861 atatgtgaac acaccagata tagttgataa ggctgttttt gaagcttttc agagaggatt
     7921 gaataagtgt gattgatatg tgttcttaaa tgtgttctta aaaacaaaag aagcactttc
     7981 caaataattg aaaagtgcca gaaacgttga tatataaagg atattaacgt tttgagaatt
     8041 gtgaagcttt acgtgctttc ttaagacctg gttttttacg ttcgaccata cgtgcgtcac
     8101 gagtaagtaa gccagcacgt ttaagagcta aacggaagtc aggatcaact tctagcaatg
     8161 cacgagcgat accatgacgg atagctcctg attgtccagc gtatcctccg ccgtttacgt
     8221 taacaaatac gtcatatgcg ccttttgttt cagtaacagc gaaaggttga ttgattactt
     8281 cacgcaagtc agcatgtgga atgtattctt caacatcttt tttatttaca gtaattttgc
     8341 cagttcctgg tactagacgt acgcgagcaa ctgcgttttt acgacggcct gtgccgatat
     8401 attgaacttg tgccaatgaa attccctcct attagattaa gtttgtgatg tctaaaactt
     8461 ctggttgttg tgctgcatgt ggatgttctg ctccaccgta tacgttcaat tttgtaaatt
     8521 gtttgcggcc taaagtgttt ttaggaagca taccttttac agaagtttcg atcaaacgac
     8581 gagaattttt agcacgcaat tcgccagcag tgattgattt caatcctcct gggtagttgc
     8641 tgtgacggta ataaattttg tcagtcgctt ttttacctgt taatttcact ttgtctgcat
     8701 tgatgacgat gacgaaatcg ccagtatcca aatgaggtgt gaaagttggt ttatttttac
     8761 cacgtagtac agatgcaaca actgttgaaa gacgtcccaa tggaacatca gttgcgtcta
     8821 ctacatacca tttacgttct acttcgcctg gtttggccat atatgttgta cgcacgttat
     8881 tttcctccaa tttaagttct gatgtttgac atacacttcg agtttccggg gctctttgtg
     8941 gggcaaacaa taccatttaa catattaccc gctaatctag tcaatgtcaa cgtttttctg
     9001 cttgattgaa aaatttatat gaaactttac ttcttttctt tcttttatat aaaagggtcg
     9061 tttcctgtca aaatgaaaaa caaaaagagg ctgtgacaaa agtcttgtca caacccctta
     9121 ttttgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat atccataaaa
     9181 cataagaagc tgttttttaa aattgtttct tgtgactcgg agctgaacgg caggcttaca
     9241 acctcttggt aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa
     9301 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatttcac
     9361 aacctctttt tcggtcaagt tcaacaacag tttttacgcg tcgatctctg gcgctttgcc
     9421 caaatgagct tggatcatcc agatacgttt ttctgtggct gttttgaatt cgataaagat
     9481 atcttgtgtt ggatcgtcgc cttcttctcc agaaacttcg attccttttt ggtaaagatc
     9541 tgctagataa cgatagcctg caacaagtgt ttccaaacgt tctggaatag tacgatccca
     9601 gcggccaatt tcatcaggga tttttgtgtt atctgcaaat tcacgcaatg tagaataagg
     9661 tgaaccgtct aatgtgatca agcgttcgga tacttcatct aactgagcgt tcaaatcatc
     9721 catgaactca tccatcattg ggtgcaatgt aaggaaacct ggtcctctca tgtaccaatg
     9781 tgtttggtga acaacaacag acatttggct tagatctgca actaattgat ttaatacttc
     9841 tttggtttgt tggaatttca tctttcttgc ctcctaacat tttcttacac ttaaaataat
     9901 agtagacttt caatgaaaag tacaacaaaa tcatttacct gagaacgatt ttcagttcgc
     9961 ttttttatcc gtaaagccaa tcatttttca gaatcagcta attcttctcg cagttgagca
    10021 aattgctgat aagtctgtgc taaatgcttt tgtgtcgaaa caaataatgg atagattttc
    10081 tgatattttt tatgatcagt ttcttgtact ccaacttgct ctttcaattc tatcaattcc
    10141 tgtgcttcac tgatactttg gatctttccg atgcttctcc agcctaaaat cacagctcct
    10201 aatgcggatg cttctgtgac agtagggaaa acaagcgggt aacccagtac atcagccatc
    10261 atctgacgaa aaatcggaga ttgagcaaac ccgcctgtag cacggatctc tgtgagctga
    10321 ttttcctggc aaactccttt taaaatccga taaaggttga gacagatccc ttctagaatc
    10381 gccttcatca tttctttttc tgtatgttgc cgctgtaaac cgataaaact gccaaacgca
    10441 tctgcgtccc aaagcggtgc tcgctcacca agtaaatagg gatgaaaaat cagaccattg
    10501 gcacccgctg gcacaaaagc gatagccgaa agcagtgcat catatggatt ttctcctttt
    10561 tctttagcag attgaacgac ttcctgtaaa aatgtatgcc ctgcccaatc aaagatgcca
    10621 gcaccattgc tagtcgctcc tcccactacc caatgtttct gatccagaac ataacaaaac
    10681 gtctccattt ctggatggaa ctgaggctga gtagtaataa aacgaagtgc tccgctcgtt
    10741 cctacggtca aagcggcgat cgtatcacca aaagcaccta accctagatt agaaagcggc
    10801 ccgtctgccc ctcccaaaac aaatgatata tcatttggca aacctagttc tgctgctgtt
    10861 tttgatggta gacctgttac ttgataggtg gaaggaaaga cttgcggcaa ttgctctatt
    10921 cgaatgttca agtagtcaag cacttcttct gcccattgga attcatggat atcaaaatat
    10981 ccagttcctg aagctgttcc gtagtcgctg atcagttcgc caaacaatct ataaaagata
    11041 tagtctttga taccgacaaa ataagcagtt tttttgaata attcaggttg gctgttttgc
    11101 agccacttga ttttgttcaa tggtgtcatt ggatgaatcg gcgtcccagt ctgccgataa
    11161 aattctttgc caagcacggt atatttcagt ttttctgctt cgtccgctgc tcggttatct
    11221 gcccaagtga tcattcgaga taacggtgtt ttctcttcat ccaacaatat caggccatgc
    11281 atcgcagagg agaaggagac aaattgaatg attgtgtctg aaggcaactg tttaattact
    11341 tcttttataa cggtgcagac accttgaaag atcaattcct gatcttgttc tgccatccct
    11401 tgtgtttctt ggatcaaagg gtagcgaaaa taagcggatg ctgcttcttg ggcttcatct
    11461 gtgaaagcaa ctgctttggt ttgtgtggtt ccgatatcta atccgattga aacgttcatt
    11521 ttttcacctc aaattttatg tatgtacaaa aatttagttt aagcttgcgt atcttggttt
    11581 tgaaaagggg gagatggcgt gttactttat tttatcattg gttgctgtat cggatcattt
    11641 ttatgtttaa ccgcccagcg gattccgtta ggtcactcta ttatttatcc tagatctcat
    11701 tgtgtcaaat gctgtcgttc gctttcttgg tatgaattga ttccgattct ctcgatcatc
    11761 gtccaacgat ttcgctgccg ctattgcagg tgtcggttgc ccttctacta tttgttggca
    11821 gaagccttat gtggtggtct ttttgcctgg ttctttactt tttcttccgc aagaaatttt
    11881 tcgacgttca tatggatgct ttcggctttg ctattttcgt tgatggattt attttacttc
    11941 atggtccatt ctcacacgct attctgttct tgggcaattt tatggatttt ttggttgcag
    12001 gccggcgtct tccaatggca aagtgtgctg ttaacgttta tcgtaggcgg tgtgtgcctt
    12061 cgctatggtc aatcctatct cggagctggc gatactctgt tgatgctttc ttggagtggt
    12121 ggcttgtctc tagaagagtt gcttcagatc ttgtttcttg ctagtctgtt tggaatatcc
    12181 gttttttctg tttactttat ctgtcataaa aaaaagttgg aaaagctgcc tttcattcct
    12241 tttttgagca gtgcgttgct gattgtcctt catcttaggt attgaaaaaa ggttgtgaca
    12301 aacgacttgt cacaacctct gatatatagt gttctcttac caaatccttg tgacttggag
    12361 ctaggcgaca ggttcacaac ctctccacta aatggtgttc aatcagctga ccctcagtat
    12421 tagctcaaaa tgctggtttt acaacctctg atatacggtg ttccataagt agctttttcg
    12481 gcttcttatt tcttgacgcc ccaatgtcga cgaaggaaac ctgcacgtcc agaaccttct
    12541 cgataaagat tgtaatggag cttgtttttc ttgtaataat cctgatggta atcttctgca
    12601 ggataaaatt cgcttcttgg ttcgattttt gtcacgattg gcttatcgaa tcgcccgctg
    12661 tcttgcaatg cttgttttga acgctctgca atatttcgtt cttcttcatc attatagtag
    12721 atcactggac gataagaatc accgcgatca gcgaactgtc ctaatgcgtc agtcggatcg
    12781 gtttgctgcc aatagatttc caccaactga tcataagaaa taacttctgg atcatattcg
    12841 atctctactg cttctgtatg ccctgttgtg cctgttgtta cttgttcata agttggattt
    12901 ggtacatgcc cgccagtata accggatgtc acagaaatga tccctggctg ctcttcaaat
    12961 ggtttgacca tacaccaaaa gcatccgcct gcgaaaactg ctttttttgt catataaaat
    13021 cccccttttt tctcatcata ccttgatttc caaaaggagg ataacgtttt gcttgtgaca
    13081 gaaaaaacgg aaactattat atacgtgata cggcttctga taaatgccaa aacgaacgta
    13141 cttgatcaaa aacggttaaa tcaggaatca catattcagc agaaggaaga tttatgtttt
    13201 cacctattcc caatacatgc atgttaccag ccaaagctgc ttgacaacca gcttctgaat
    13261 cttcaatgac caagcatgca tttggcggta cattcaattg ctgtgctcct ttgagaaaaa
    13321 cttctggatc aggttttgct tttgatactt gcgtgccgtc aataaggaca tcaaaataac
    13381 ttgtcaaatt cgttttttct aaaatgaggc gtgcattttt gcttgctgat cccaatccga
    13441 tcttgatgtt tgtttttttc aagtactcaa gaacagctat tgcaccaggc aaaacggatg
    13501 tttcatccat tttttgaatc gcttgaagat atagattgtt tttttgctca gcaaaggctg
    13561 atttctcttt ttctgagaat ttctgttcca tttttccaaa tgacagcaat cgctctagtg
    13621 actcggttct ggatattcct ttcagttgct cgttttcttt ctcagtgaat ggaatggaca
    13681 gctggttagc taaaaccagc cacgcttcgt aatgataacg agctgtgtct acaagaacgc
    13741 catctaaatc gaatagtgct gctttgattt tcatcgatta cctccttgaa aatgaacgac
    13801 ttgaagtaaa gatgcgatgt ttttagctgt gcgatgaaga ttatcagtgg cttgctgtaa
    13861 agcttcttgt aaaggcacag ctgcaggaag gatcggaaaa acggccgaaa tgccaacatc
    13921 tgctgctttt tcgcttcctt taccaacact tccgcaaata gctatcacag gaacgccctc
    13981 tggtactctt ctagcaatcc caactggcgc ttttcctttt agactttgtc catctagacg
    14041 tccttcgcct acgattacga ggtctgcttg ctgtacccgt ttatcgaact ccagtaaatc
    14101 caatacgtag tcgatgccag agactatttt ccctgaagca aatgctaaca gtcctgcacc
    14161 cattccacca ccagcactac tgcccgatgc ctcgattact tgaggggcaa atctcatata
    14221 aaaatcatgc atggcttggt cagccactgc taattcattt tctttcaagc ctttttgtgg
    14281 tccgaaaaca aaagaagcgc cttcttttcc gcataaaaga ttagtaacat cagtaatgac
    14341 agtgattttg gctttaatca gctcaatagg cacgttcgtt ttatcaactg tagcaatttt
    14401 acctagattt tctcccagtg cttctacttt ttgattatct tgatcgtaaa aagcataacc
    14461 tagaccttct gccatgccga tgccgccatc gttggtacta cttccgccaa cgccgatgat
    14521 gatgtgtcga acaccttgtt gcaacaattg tcgtatcagt tctcctactc ctcgcgtgga
    14581 taacctaagt ggctgttgtt ggtttagtgg gacatctttc aatccgacga tttctgccat
    14641 ttcaaaaatc gcagtttgac cgtctgaagc gtactggatt tctttgtttt tgccaaatgc
    14701 attagtaacg actgctgttt gccgcttcaa ccttaaactg tctgctaatg atgaaaccgt
    14761 tccttctcct ccatcgccta taggtagcag atcatattca gcctgaacaa aaacttcttg
    14821 aaaacctgat tgaatggctt gtgctgcctc taaggctgtc agactttctt tgaaagaatc
    14881 tggcgcaatc agaatacgca ttattttccc ctccgttcca agatcagttc tttttccagc
    14941 tggtattggt ctccattgac aaaaataggt aatgtttctt tcgtctcttt ttttattact
    15001 atttgttttt ttgtcaaaac aaatagcaac ctgtgatttt tccaaataaa aggaaaagaa
    15061 attttttccc atgctgaagg aagtcttgga gaaatagaaa gagttccatt cgttactgta
    15121 atacctgaaa aaccaaatat aactgctagc catagtgctc ctaatgaagc agcatgaatt
    15181 ccgtcatctg atgaatgcgg atctggtcct aaatcaatca ggcacgcttc ttggaataaa
    15241 tgatacgccc attccatttc ttggatacgg caagcctcta tggcgtgaat cgctttactc
    15301 aacgatgagt cgtggattgt tttgttttca tagtaatgca aattcttaga aacgattggt
    15361 tgagaaaaat actcaggtaa taagtataac agcatgacaa gatctgcttg cttgctgact
    15421 tggagttcat ttacttcgct acgggcataa tcaagtaaaa tagactggct gccaggacgt
    15481 tctttgtaag tggtcaaatc aataactggc ttactgagat acgtgtcatc ttgcggaatc
    15541 agctgttctt tgttaggcac tggcaaatat aacttatcta aaaaattttg acattctttc
    15601 aaaaaaagcg gatcgccagc ttgatattta acagctaagg ccacattata atgcgccaga
    15661 tagttcgtat atgcattatt gtcgatatat tctgtgtatt catctggacc aatgactctg
    15721 tgtatctcta aatgatcatc gacttttaca gctctgctca gccaaaaagc tgctgtttca
    15781 tgaatgagcg tttttccctt tgtttctaaa aacgattgat cctctgttgc ttggacatac
    15841 tcttgaacgg cataagcaag gtctgcactg atatgatgtt ctgcggcagc tgaagcaact
    15901 tcttgtcttg ttccggtttt gatattcaat gcagcaaatt tgggtgtctc ttcctgacca
    15961 gtgtgtgcgc tttcccatgg gaaaagtgca ccttgatagt gataatctct tgctttttgc
    16021 cgtgcgcctt ctaatcgatc ataacggtac agcagcaaat tctttgctac ttcaggaaac
    16081 tgatataaaa agaaaggcag gataaaaatt tccgtatccc agaaaacatg ccctttataa
    16141 ccttcgccag ttagtccctt cgcccctaca ctaacacttg gatcattgcg gggagtcata
    16201 atctgtaaat ggtaagcagc gaagtccatc gctaactgat cgaaagaaga atcgccttgg
    16261 atcgttatcc gatgctgatg ccaaaaatct gtccaataag acatcgactg ctctaaaatt
    16321 tctgtataac taacgctttt ctgggcttgg gcaatacttt tcttaaacgg gtctgtttgc
    16381 tgaagagagg tagagagcac gatgatcttc tccagcgtaa aggacgtatt ttttctgatc
    16441 ttttctgaaa aggaagcggt gatccggcga ttttttgctt ggaatacacc tgctttattg
    16501 aatttagctg caatcccaat atcaatttta ctttctgttg tctggtagga tgcactgatc
    16561 aaatcgtcac caaatactcg cagttctttt tcaaccaact gctgtgtacc aaaattggtt
    16621 tgctgtccat cgatacccgt tttcacatct acttggcagt cttcggtcaa cggcttgatg
    16681 accactttca gggctgcctg atggagatta ttttgatcag caacacgaca agtaatgact
    16741 tccacttttt ttccttgagc agtttcaatg agcgcatagc gtttaagctc tcccatttct
    16801 aaatctagtg cccgctcgta tgcaagaact ttcatcgtct caagagaaaa atgttcaccg
    16861 tcgattttta tttcccatct gatgacatct gggagattca ctaactcggc tgattctgct
    16921 cctaaaggtc ggttatatac accagctgca aacattcccc ttacttgatt ccgatagtct
    16981 tcctcctggc ttcctctgat accaaaaaat ccatttccaa tggtcatctg actcgcatac
    17041 ttattgatat actctaaatc aaactgtttt tcttctactt tcataaggta ataacttctc
    17101 ctgtttctgc cgaacgatat aaattttcca ccagattctg gatgacatat ccttgccagc
    17161 catctgcaat catctgagtc tggtcaccga gcgcccgatt cacaaaatga gccatactta
    17221 gttcatgtgg gtcattaact ggcgtggtct tttgtaagga aaccagcttt ccatctacat
    17281 cttcgaatag ttcgcctgga tacagattag cacctgcctg atcgccacaa aaagccacat
    17341 tcagtttgtc ttctctgata ttcaaagcaa acgaagtatt cagttggatg atcttgccgt
    17401 tttctaattc cagtgtgcca aaaattgcat cttctactgt gaatttgctt ggatcccatt
    17461 tcccgaattt ccctgaattt tttttcgtac caattttttg gaagctgtac gcccgaacgc
    17521 tttttacttt aggaaatcca gtaatataca tcgctgtatc aagcatgtga atgccataat
    17581 cgatcaaagg cccgcctcct tgaagttctt tgtcgataaa cgttccccag cctggaacac
    17641 cagaccgacg aagcgcattc gcttctacgt aataaatatc cccgagaaca tcaagatgat
    17701 tcttgatcat tgttgcttcg tcagaaaaac gatgctggaa tccataagcc aaaatctgcc
    17761 cgtttttatc ggctgtctct gccatctttc ttgcttgatc agctgtcata gctggtggtt
    17821 tctcgcacat cactgaagca ttagcttcga gggcagccaa tacatgttcg aagtgaaagc
    17881 gattgggtgt acagacactc acgatatcgg gagaaatctc attcagcatt tgtgtaactg
    17941 atgtatacgc atgagaaaat cctgtacgtc ggcaaaagtc ttctgctcgc tggctgactg
    18001 gatcaaccac agcggccaat tctaagacat ctgatctttt ttgataataa gcagcatgta
    18061 ctttttctgc tacttgtcca gcgcctatga ttgctacttt cttcatttat ttctctctcc
    18121 tttgcttatt tacttattgt tattctttct ctattctttc tctaaatcat ttaaatgacg
    18181 cgctgtttcc agacagtttt tcaagtattg caatgattcc tgataaattt tcacttcatt
    18241 ttctcctcgc aaacgacatt catataccat ataaccttca tactcatttt gaagtagcgt
    18301 ttgaaaatgg cgagtaaaat caattgcgcc agatcctggt tggtagcgat gattatctgc
    18361 taaatggata tgacccacat actctttttg cgtagtcaat gcttttgaaa tatcatcttc
    18421 ttcaatattc atatgataaa aatcagcgat gatctgcaca tgtttcaatt gattagagtc
    18481 aatgtaatga cgcgcatccg ctaacaaatt gatcatatgg tcttggtatc ggttcaaagg
    18541 ctccaaataa ataaccgtac ctgtttcttc ggctacttta tctaaataac gaagtgattc
    18601 gctgactgct ttatagtctc cttctttgct tcggggtgaa accatcggcg gtaatcgata
    18661 agtgaacatt ccccaagctg ctgggataac gattcctttg cctccaacct cgcttagtgc
    18721 acgcaaaatc tcagcaattt gacgtacacc attaagacgc cgttcttcga taaagtcacc
    18781 gatccaccca tcgtagccgt tacaagctgt cactactggg atccctgttt ctttgattgc
    18841 ctgtttgact gtgtcgatat tttctattaa caacttaccg tcgatttcgt attcttcaaa
    18901 tccacagtct ttgataaatc taaatttttc taaaatgtct cttgggaaaa atgcttgatc
    18961 ttgcgtaccg aatttcattg ttctcctcct cggacttgaa cgcccatttt gatactttct
    19021 tctgggtgct ggtctacata acgcatgtaa gcttctgcgc tttctgcaaa tggcacaact
    19081 ggatcaatga tttcttcaca atccaagtaa ccgttcatca ataattccca acatgtgtcc
    19141 tcgattcgtt tacggttcca tcttgggtaa tctgggtttg gttcactaga tgctcgagca
    19201 aagacgattt ttgcattatt gaaatgtgct tcgcgaccaa gattaaaccc agctggaaaa
    19261 ggtttagcaa aggcaacata agaaatgatc ccgccatagc cgatccctat taaagaagct
    19321 tgtaatgcgg cagcactgcc actcgtttca ataactgaat ctactccacg tttatctgtc
    19381 aattccttta gacgaaaacc gacatctgct ttcataggat ctagcgtgta atcggcaccg
    19441 tgtttttctg ctaattctct acgctgcgct atgggatcga tcccaacgat ggtagtagca
    19501 cctgcttttt tagctaattg aatcgcaatt tgcccgatag cgcctaaacc gaagattgct
    19561 acagcatcac ctgctcgaac atgtgcttcc cggataccac ctaaagcgaa ttgagcagga
    19621 tcgtaacata ctgcattttt ccattgatcc aaccgatcaa gtttacgtag tttataatta
    19681 tcaacaccgt ttgtaatcag cgtttcacga atgggtccat aagtgcagac taactctcct
    19741 ttttgatact ctgtcaccaa actgcccgtt tcttcgatcg taccgacaat catatttcct
    19801 aattgaaatt cgccaaatac cacaccgcgt tggcttcctt cttctcttgg caagaatagg
    19861 cgccattctt ctgaaaattc ttcatcgata aatggactta atcctctaaa gtcgacaatt
    19921 tctgttcctg gttttggagt agcgaaaatt actcggatac gtacttcgtt cttttcgatc
    19981 tcacggtctt cgtaatcttt gaatccggct attcttggtt ctaatgcaac taattttttc
    20041 atcttttctt tcctctttct tttcttttta tccttttacg ccgccttcga ctaaatcacc
    20101 tttaataaat cgttcagata acgcatacat aatgatgact ggtaaagcgg tcaccaatga
    20161 agcggccatc attcgtcccc aaatgtagtc tggtgtactg aataatgtgt tcaaaccgat
    20221 tggtaaagta aatttttcgg aggtcgacag gaaaatcgaa gcaaataaat aatcattcca
    20281 cgaaaccata aagcaataaa cgaagacaga aatgattcca gaaatagcca atggtataat
    20341 gatccgaata atgatctgga tccggctaag accatcgatc attgctgctt cttcaatatc
    20401 agtcggaatc gtgtcaaaat aactttttag catgaatatc gcagtaggaa gtgtttgaac
    20461 gaccatcgtg ataatcagtg ctgtattggt atcatacaaa cctagaccgg aaatgatttt
    20521 aaataatgga acaacgatca aaataccgga aaacatataa acggtgtaaa agctagcatt
    20581 gattttcgag cgagctttga agcgtagttt tgacaaagcg tatgccccgc tgatcccaat
    20641 cactactgca gttacggagg ctatcaagga gatcactaaa ctatttttga aatacatgat
    20701 gaatggaaaa atctcagcat tgaaaatatc ctgataatgt tcaaaagtcc attctttcgg
    20761 aaaaatagtt tgtgtcgttg caattgcttc ttgtgaatcc ttgaatgatg tcatcagcat
    20821 gatcaaaaat ggaaacaaat tgacagctaa tagcaacaaa atcgtcgcgt aaaataagat
    20881 tttcctgctc tttttatttt tcatttcaag tccaccttct ttcgtgagag caagacgacc
    20941 gctgcaataa tgatgaacaa tagaatcgat atagctgctg cttttccaag atcattgaac
    21001 gcaaaggctg tctgatacaa ataaacgcct aagatattga ctttattcgt cagtagatag
    21061 acatctgtaa acatatagaa catccagata gatcgtaaaa caatcacagt tgctagtact
    21121 ggtttgattg ccggcagtgt cacgatcttg aacttctgcc aagcatttgc accgtccatt
    21181 tcagctgctt cataaagcgt tttgtcgact gtttgcaaaa tagccaaaaa tgaaataaac
    21241 gcatacggat agtatctcca gatagcaaac aaaacgacca taaagaagct agcaactggc
    21301 tggtcgaacc acagcggtgc ttcgttgaaa agctgcaacc tatctactaa cagatcattg
    21361 acgattccat aactattatt gaacatatat ttccaagcaa aaatcaaaga aatagatggc
    21421 gtaacataac ttaataaaat cagtgaacgc accgttttcc taaatctgaa cttgcggtta
    21481 agcaaaatag ctacggctaa tcctaaagct gtactgcctg ctacaactaa gaatgtgtaa
    21541 gcaactgtta aacctaaaga acgataaaat tcactatcac ttaaaatttc aagataattg
    21601 cttaatccga taaaaacggc gtctgagtta ggattcagcg gcatatcgaa aaaactgatc
    21661 tgaatattgg aaagcattgg aatcagcact aaaacagcta ataaaagcat gcttggtgct
    21721 aaaagtccca tggctaattt taagtcagaa tgtttccttg gacgattcat tgaaacctcc
    21781 ccctgataat ctgccaatag ctatctactt ttttcgaaca ccattggcag aacttcttgt
    21841 tatttcgtat ttttttgtgc gtcagctaaa gcttgatcga catcttcgtt tccgacagtt
    21901 acttggttca ctgcttttcc gatcacacct gagcttgtga tatctcccat tttcgtgaaa
    21961 ttcttttccc cgactagtcc aaagacttgt acttcatcaa aagcactagc aatctcagat
    22021 ggcagctcgc caaaagactt gatgacttca ttttctttgt aaccatccag ttcgactact
    22081 tgtttattca ctggttgagc accaccagga gacatcaaga cccacttttc catgtttttc
    22141 ggttgagata agaaagctgt gaatgcttca gcagcctctt tttgtttctc gtctagacca
    22201 gaagaaatcg tcaatccaga tactgtgcca tagacagctt gatctttttg tgttgggata
    22261 gcaaaaccga tattttctga gttccccgct tcataaacag aaggcaaaat gtaagttgaa
    22321 tagattgcca ttggtgctgt tccattcatg aaagcatctt tgatttctgt cacatcatta
    22381 gagcctggca tcgtgtattg ggataagttt tgataaaacg acaatgcttc tctcatcttt
    22441 tccgtatcga tcgttacctc gccttcatca tccaaaacat ttgctccatt tgacaaagca
    22501 aattgagaga acgcttgctc actcattgtt ccttcagcag taggcatagc aattccatat
    22561 tgtttattct ctttatctgt gaaatgttga gcaattttta aaatatcatc ccaattttct
    22621 ggttctgaaa aaccagcttc tgataatttt tctttgtcat accaaatacc ttgaacccag
    22681 ccgctaattg gtgcagcaat atagcttttt ccatcttctg aacgaacaag gtttttggct
    22741 ccgtcataat aattatcttc tccagcttcc tcgataacgt tttggactgc cgtctgatca
    22801 atcagttcat ctttatccat cactttagca aaatcctgac tgacttccat tacttctggt
    22861 aatttaccgg aacgagctaa cgtaacgatt tttgtattga acgctgattc ttctaccggc
    22921 acttgcttga ttttgatgtc tggattttct ttttcaaaat cagcaaccag ttcattgatc
    22981 acacttaatc gttcctgttc gacagaagag tgcatgaatt gaatcgttgt ctgttgtgat
    23041 tcgttagagc tttgactttc accacaagca gtaagaagaa atccagtagc tagcagacag
    23101 gtagtaacaa taattttttt catttttttc cacctcttat tttattattt tcagccacat
    23161 atattcatac ggctggaata tcaactttcg ttcttgactg caatggataa tttgtccaga
    23221 aaacaggtct aggtaatctc cttttggtaa aagataacct gctgattgat tggaaaaatt
    23281 atggataact aagactttct cttccttcgc ttcgcgaata aaactgataa aagaatctgc
    23341 tgagtccagt aagtaaatca aactattcgg atggaaggct ttttcttttt ttcgaataga
    23401 tagcatctgg gtcaattcac catatactgc tgttcttaat ggatctgtct gcaactcttt
    23461 ctcaatatct gctaacaaaa atttttgccg attgattttt cgattctctc ctgttttttc
    23521 tacgcctttg taatcatttc gacttcccag cacactttga atataaattg ccggcacgcc
    23581 tggtaacgtt accaaaaatg aatgagcagc taaaaagcgt ttgcttctcc attccgtcgt
    23641 atctgttgga gaacttaacg catctagata cgtagcgttt atctcatatg gactggtcgt
    23701 tccatctggg tttttcttgt agtttactcg ggcaccctct ttcttcagct gttccactaa
    23761 ttcaaggatc gttttttccg gcagaatacc tcgtaccgga ttcaatccga taccgtcatg
    23821 ggaggctaaa aaattaaaaa aagttgtttt tttcgatgcg aaatcgatgg tttttaacca
    23881 ttctttcaac ttacttgtat tacccgaacg aatcgcttga agggtcaaag gtggtagcga
    23941 aaactgatag accatctgtg cttcattctc gccattccca aaataactga tattatcttt
    24001 gtgtggcaca tttgtctcag tgatcaatac cgttcctgga gctgcttcat cagcgattgc
    24061 acggaataat tggatgattt tatgcgtttc tggcaggtgt atacaagaag ttcctagttc
    24121 tttccataaa aatcccacag cgtccaagcg aataaatgaa gcgccttttt ctaagtaaaa
    24181 caaaagaata tcgatcattt tcaataaaac ttctggattt ttaaaattca agtctacttg
    24241 atcttcgcta aaagtcgtcc atacgtgttt ttctttcccg tttttgtcaa tgaacgttgt
    24301 aagtaacggg ctggtccttg gtctagtcac gctgcttgta tctgtatcca gtggcaaatc
    24361 aataaaaaaa tctttgtacg tatcattcaa gtttaaatat tcctgaaacc aagtactttc
    24421 ggcagacata tgattgcaga caaagtcgaa cattaaccgt gtttcgcttc tcatttcttc
    24481 tatatcttcc catgtgccca aacggtcgtt gattgcctga taatttacaa cggagaaacc
    24541 atcatcagat gtatatggga aaaatggcaa gaaatgtaca actctaaatg tatctgccaa
    24601 atacttttga taaaatttct taaatgtacg caaagttggt tgctgctctt caaaaaattg
    24661 atccccataa gtaatcaaga aaatatcttc ttcgtcccaa tgcagtttgc ctgcctggcc
    24721 tttcttctgc tgataaacac tgactttttg ttcaatcata gaggcggttt tctcagacaa
    24781 atctgagtcg cgataaatcg cttgtaaaag ttttgtatac atcggattcc cctttctttt
    24841 ttgggagcgt tcccaacgat ttgatcatat tatggaaaac gcttactgtc aatagcattt
    24901 ttttaaataa ttttgatata cacaaaaata caaataagaa agcttatgta tcaataatta
    24961 atagcttctt aaaaaaatat ggtctctcat gatcatgcaa gctattccta aattttcaaa
    25021 atcatagaca aaaaagaatg tctattgtac tatggaatta ttctaaaacg ctcccaaaaa
    25081 ggaggtgcat ataaaattga aacctactat ttatgacatt gcgaaaaaag ctaatgtctc
    25141 aaaatcaact gtctccagag tattaaataa ccagccaaat atctctgata aagcaaaagc
    25201 aagtgtactt caagcaatca aagaattgaa ttatgaaccg agcaaaattg caagaggact
    25261 gtcaggaaat ggttttgacg cgattctagc attatctcat cgtacttctc atacgaccta
    25321 tggcaatcct tttttttcag atgttattca atccatttct gccgtttctg aacaaaataa
    25381 cttcgacttg attctacaaa cggcaaaaac accagaagaa gagctagaaa aatgcgcgaa
    25441 aaagatccaa gaaaaaatga tccgaggaat tattatttta agctctacga tggatgaaac
    25501 acttcttttt gaattggatc aatataatat tcctattgtg gtaatcggaa aagtcggaaa
    25561 cgactacacg aacatctact cagtggacac ggataatttc caggatagtt acgatttggt
    25621 ggaaacactg atcaagaatg gacaccgtca tatcggctgt ctccatgctc cgacagaagt
    25681 ttatgtatcc aatgatcgtc tcaatggtta ccgtcaatgc ttatttgatc accacctaga
    25741 tatccgtaat gaatggatta tagacggcgg ctttcgctta gaagatagca tggcagcaat
    25801 gaatgattta atgaagctac gagaaagacc aactgcgatt ttttctaccg atgcattaaa
    25861 atcattgagt atttacaaag tggcagaaca atctggaaag cagatacctg atgatctttc
    25921 gatcgcagga ttcagtgatc ccttattgtc ctccctattt tcgccacagt tgactcggat
    25981 agacattccg acaaatcagt taggaatcaa agccacagaa cttttattcc aactaatcca
    26041 ccaccaatac cctgaagaaa aaagaatcat tctttcaacc gagattactc tcacagattc
    26101 gatccaacaa caagaaatgt agaacagaat tataaaagaa cagaaacact gaggataagt
    26161 cagttactct gttctttttt caatcactca tccctcgtta ccccctcccg gtattttagc
    26221 gaatttttct tattttccat ctgttttaga tagctgtgga taactataat gtttgtgtta
    26281 aactattttt tccacaggga agaaaaaact atttttaacc acgttgtgta taagttttta
    26341 tagttcgttt ttttaaacac atttaccctt atttcaatcc atgttatttg gggatactta
    26401 tctctaaaac ataaatagaa taacagacat gtctactggt tttgttttat tcgaggaatt
    26461 attcaataat agctagcaaa atattaaaaa taaccgctct atcttcccat caatttattt
    26521 tgcattcaaa aaatgatcta tctttacttt tgttgattga cttctcttgg aagatgatcg
    26581 ttccgatacc ttaattggat acgaaccgat gttgttaaga tcctttccca cttttcttct
    26641 agttagacaa attattttag acaaaccaaa aaagaggttg tgacaataat ttgtcacaac
    26701 ctctttttta aaatatctat tttgtttgat tgttcattgc gtcttctgca gccatttgcg
    26761 cttcaatatc tgaaatactg tagacatttt cgcttgctac accgacttct tttggttcca
    26821 tgtcacgata acgttgcata ccagtaccag ctgggatgat cttacctata ataacatttt
    26881 ctttcaaacc aagaagagga tctttcttac cgcggatcgc agcatccgtc aagacacgag
    26941 ttgtttcttg gaatgatgca gcagataaga aactatttgt ttctaatgat gcttttgtga
    27001 tacctagtaa gacaggtcga gaagttgcag gaacgcctcc tgagactagc gtattgtagt
    27061 tttgatcttt aaagtctgcg atgtctaata atgtacctgg taagatatct gtatcacctg
    27121 gatccattac acgaacttta cgcagcattt gacgaaccat tacttcgata tgcttgtcgc
    27181 cgatttctac cccttgcata cggtaaacac gttgtacttc acgaagcaag tagttttcaa
    27241 cagataatac atcgcgaact tgcaacaatt gttttggatc gattgatcct tctgtcaatg
    27301 gtgcaccacg gtggatcata tcgccttctg ctactttcat acgtgctgta taaggaacag
    27361 tatacgtacg tgtatctgtt tttcctttga ttgttacttc tttggtacgt gttgcaggat
    27421 cttctgagat atcgatgaca tcccctgtta cttctgtgat cactgcttgc cctttcggat
    27481 tacgtgcttc aaagatttct tggacacgag gaagaccttg agtgatatcg tctccggcaa
    27541 ccccacctgt atggaatgta cgcatcgtca actgagtacc aggttcaccg attgattgag
    27601 cggcgatcgt accaactgct tctccgactt caacgtcaga gccagttgca aggttgcgtc
    27661 cgtaacaatg tttgcagaca ccatgttttg tattacatgt gaagacagaa cggattgtca
    27721 ctgtttcgac acctgcatca acgatttgtt ttgcgatgtc ttcggaaatc aattggtttc
    27781 ctgcaatgat caattcattt gtttcaggat gacgtacttc tttacgggtg taacgtccca
    27841 ctaaacgatc ttctaatgat tcgatgattt catttccctc gcggatggct tggatttcaa
    27901 gacctcggtc agtaccgcaa tcttcttcac ggatgattac atcttgggcc acgtcaacca
    27961 gacgacgagt caagtaacct gaatcggcag tcttcaaggc tgtatcggtc atccctttac
    28021 gagccccgtg agttgagata aacatttcca agacggatag tccttcacgg aagttcgaaa
    28081 tgattggcaa ttccatgatt cgaccattag gagcggccat caaaccacgc attccggcaa
    28141 gctgtgtaaa gttggaaatg ttaccacgag ctccagaatc agacatcatg aagattggat
    28201 ttttcgcttc caaaccttcc atcagttttt gttggatgct gtcttttgca gcattccaaa
    28261 cagcaatgac acgttcataa cgttcgtcat ctgtgatcag accacgacgg aattgttttg
    28321 taatagtttc aacttgttta tgggcatttt caatgatttc ttgtttttca ttcagtacac
    28381 tgatgtcagc gatccctact gtgataccgg catgtgtcga atgtttgtac cctaagtctt
    28441 tcatacggtc aagcatctta gaagtttctg ttactttgaa tcgtttgaag acttctgcga
    28501 tgatgtttcc taagtttttc ttcttgaacg gtaatactaa ttcttgctct ttgatatgtg
    28561 caggaatatc agtaccagct tcaacaaagt atttgtccgg tgtttggacg gtcaagttga
    28621 agtctgttgg ttcattcaga taagggaatt caggaggcat gatctcgttg aagatgatct
    28681 taccaactgt tgtgatcatc gtacgttctt tttgccattc agtaaatggt ttttcaccta
    28741 atgagttagg gttcacaccg atacgtgaat gtaaatgaac atatccgttg cgccatgcga
    28801 taacggcttc gttttgatct ctgaagacca ttccttcgcc ttcgcgtcct tcttcttcca
    28861 ttgtcaagta gtagttacct agaaccatat cttgagaagg agtaacaact ggtttaccat
    28921 ctttagggtt caagatgttt tgagcagcta acatcaacat acgtgcttct gcttgtgctt
    28981 cttcattcaa tggaacgtgg acagccattt ggtctccatc gaaatcggca ttataagctt
    29041 cacatacaag tggatgcaaa cggattgcgc gtccttgtac taaaactggt tcgaatgctt
    29101 ggataccaag tcggtgaagc gtaggtgccc ggttcagcaa tactgggtgt tccttgatta
    29161 cgtcttctaa aacatcccat acttcgtctt cttgacgttc gattttacgt ttcgcatttt
    29221 tgatgtttga agctagttcg cgttgaacaa gctcacgcat cacgaatggt ttgaataatt
    29281 caatggccat ttcttttgga agtccacatt ggtacatctt caagaaaggt ccaacaacga
    29341 taacggaacg acctgagtag tcgacacgtt tacctagcaa gttttgacgg aaacgacctt
    29401 gtttcccttt caacatatga gaaagagatt tcaatgggcg gttacctggt ccagtaactg
    29461 gacgtccgcg acgaccgtta tcgatcaatg catctactgc ttcttgcagc atacgttttt
    29521 cattttgaac gatgattcca ggtgcattca agtctaacaa acgtttcaaa cggttattac
    29581 ggttgattac acgacggtaa agatcattca agtcacttgt tgcaaaacgt ccaccttcta
    29641 attgaaccat tgggcgtaaa tctggtggaa taacagggat aacgtccata accatccagc
    29701 ttggttgatt tcctgaagcg cggaatgctt ctaaaatatc caaacggcgg attgcacgtg
    29761 tacgtttttg tccttgtgca gatttcaatt cttctttcaa ttcggctgct tctgcatcta
    29821 gatcaacatt atcaagtaat tgtttaatcg cttctgcacc catagcagct tggaattctt
    29881 gtccatattg ttcacgtttt tcgcgatatt cgcgttctgt caacaattgt tttttctcta
    29941 aattcgtatt tcctggttcg atgactacat atgaagcaaa ataaatgatt tcttccaaag
    30001 cgcgtgggct catgtctaat acaagaccca tacgtgaagg aatcccttta aagtaccaga
    30061 tatgtgaaac aggagctgct aattcaatat gtcccatgcg ttcacggcga actttcgaac
    30121 gagtaacttc cactccgcat cgatcacaaa cgattccttt ataacggata cgtttgtatt
    30181 ttccgcaggc acattcccag tcttttgtag gaccaaaaat ccgctcacag aacaaccctt
    30241 cacgttcagg ttttaaggta cgatagttaa tcgtctcagg tttttttact tcaccgtaag
    30301 accagcttct gattttttcg ggagaagcca aacctatttg catactttcg aatttattta
    30361 catcgatcaa aaggggttcc ctccatttca tttaatggca aaccgcaaga cggctgatct
    30421 tcgaccgcct tgcagctcat agaccggatt agtctaaatt atcttctgcg gtttcaaagt
    30481 tttggatgac atcgtctttt tcatcttcca cttgttcagc agctttcttt tctaattctt
    30541 tggcagtttg ctgttcggca aattttgtca aagcatcaac agtgatcaaa tcgtcgtctt
    30601 catcgtccat gtcgcgaagt tcgatctctg tttcttcgat atccaagaca cgcatgtcca
    30661 gacctagtga ttgcaattct ttcaccaata cgcggaatga ttccggtaca cctggttttg
    30721 gaattggttc acccttgacg attgcttcgt atgttttcac acgtccgact acgtcatctg
    30781 atttgtatgt caagatttct tgtaaagtat aagccgcacc gtaagcttcc agtgcccata
    30841 cttccatttc accaaaacgt tgtccaccaa attgagcttt acctcccaat ggttgttgtg
    30901 taaccaatga gtaaggtccg attgaacgtg catgcaattt gtcatcgacc atgtgagcaa
    30961 gtttgatcat gtacatcaca ccgacagaca cacgtccatc aaatggttca cctgtacgtc
    31021 cgtcgtacag aacagttttc gcatcgctgg ccataccggc ttcacgaact gtttcccata
    31081 cgtcttcatc acttgcacca tcaaatactg gcgttgctac gtggatacct aactgacgtg
    31141 cagccatacc caagtgtaat tccaatactt gcccgatgtt catacgtgaa ggcaccccta
    31201 aaggattcag catgatatcg attggtgttc catcaggtag gaatggcata tcttcttccg
    31261 gcataatacg ggatacaacc cctttatttc cgtgacgacc ggccatttta tccccttcat
    31321 ggattttacg tttttgaacg atataaacac gaactaacat gtttacgcct ggtgacaatt
    31381 catctccagc ttcacgagta aagattttca catcgtgaac gataccgccg ccgccatgtg
    31441 gtacacgtaa tgatgtatcg cggacttcac gagctttttc gccaaagatc gcatgcaata
    31501 gacgttcttc tgcagataat tctgttaccc cttttggcgt gactttccct acaagcaaat
    31561 cgccatcttg gacttctgca ccaatacgga tgatacccat ttcgtctaaa tcttttaatg
    31621 cgtcttcccc gacattaggg atttcgcgtg tgatttcttc aggtcctaat tttgtatcgc
    31681 gggcttctga ttcatattct tcaatatgga tagaagtgta tacgtcatct tttaccaaac
    31741 gacggctcat gatgatcgca tcttcatagt tgtacccttc ccaagtcatg aaaccaacta
    31801 aaacgttttg gcctagagcc atttcgcctt gttccataga tggtccgtct gccagagtat
    31861 cccctttttc gactttctca cccaaatgaa cgattggacg ttggttgtag cttgttccgg
    31921 agtttgaacg acggaatttt gttacatcgt atttatctaa tgcaccgttg tcgcggcgta
    31981 cgcggatttc agaagcatca acgtattcaa cgacaccgtc atgtttacat aacaaagcag
    32041 ctcctgagtc atgcgctgat ttgtattcca taccagtacc aacccatggt gcttgaggat
    32101 tgatcaacgg cactgcttga cgctgcatgt tggcacccat caaggcacgg ttggagtcat
    32161 cgttttccaa gaaaggaata catgctgtcg caacggcaac tacttgttta ggtgatacgt
    32221 ccatgtagtc tactttatca atagatactt ctaagttttc actttgagca cgagccataa
    32281 caacgtcttg agcaaatgtt ccatcttcat tcaatgggct gtttgcttgg gctacgatgt
    32341 aatggtcttc gatatcagct gtcaagtagt cgatttggtc agtaacacga cctgtttcac
    32401 ggtctacacg acgataaggt gtttcgataa atccgaattt atttactttc gcataactgg
    32461 acaaactatt gatcaacccg atgtttgggc cttcaggagt ttcgatcgga cacatacggc
    32521 catagtggga gtagtgaacg tcacgaactt cataaccggc gcggtcacga gtcaatccgc
    32581 caggtcctaa ggctgaaaga cgacgtttgt gtgtcaattc gccaagaggg tttgtttgat
    32641 ccatgaactg tggcaactga gaagaaccaa agaattcttt gatacttgcc acaactggac
    32701 ggatattgat caattgttga ggtgtcaatg tttcagtatc ttggatcgac attctttcac
    32761 gaaccacacg ttccatacgt gctagcccga tacggaattg gttttgtaaa agttcaccga
    32821 ctgaacggat acgacgattt cctaagtggt cgatatcatc gacattgccg ataccttcca
    32881 tcaagttcaa gaaatagctc attgaagcaa tgatgtcggc tggacgaacc gtttttacag
    32941 ctgcttctgg atagccgttt ccgatcacgt ttacttcacg ttcagggtct cttggtgaaa
    33001 atactttgat gacttggaca gtcattggat ctgtgaccac accgtcttct gatggatagt
    33061 aagtcactga atttaggcca ttatcaatga aaggagctag tgtttccatg acttggtgag
    33121 tcagaactgt tcctttttca acgatgatct cgcctgtttc tggatcaact aatgtttcag
    33181 ctaatgtcag attcaataga cgtgttttca aatctaattt tttgtttact ttgtaacgac
    33241 caacgtttgc taagtcgtaa cgttttggat cgaagaaacg agcattcagc aagttacgtg
    33301 agctgtctgc tgtttttggt tcgcctggac gcaaacgttc gtaaacgtct ttcaaacctt
    33361 cttctgttct tgagtcgctt gcgtttttat gcaagtcttt ttcgactgta ttgcgtaatg
    33421 tttcgctgtc gccgaagatt tcaaagatcg tatcatcgga accaaatcct aacgcacgaa
    33481 ctaatacggt taaaggaatc ttacgtgtac ggtcgatacg tacataagag atgtcttttg
    33541 catcagtttc catttctagc caagctccac ggttagggat aacagtagaa ccgaatcctt
    33601 cttttccgtt tttgtcgact tttccatgga aatagacacc tggtgaacgg actaactgag
    33661 aaacgattac tcgctctgcc ccgttgatga tgaatgttcc ttgttctgtc attaatggga
    33721 aatcaccgaa gaatacttct tgtgctttga tttcgccagt ctcgcggttt gtcaaacgca
    33781 atgttacatg taatggtgca gagtagtttg catcatgcgc acgagcttct gctactgtat
    33841 actttggttc tttcaattca taatctacaa attccaaaga tagattgcca ttgaagtcat
    33901 cgattggcaa aatgtcttca aacatttctc taagtccctc atctaagaac cattggtaag
    33961 agtctgtttg aatttcgatc aaatttggta attccaacac ttcactgatt cttgcgaaac
    34021 ttctacgttc gcgatgtttt ccgtatttta ctacgtgtcc agccaagctc ttcacccctc
    34081 taaaatgtca ttcaaaaaga ctacccgatc aattgttaca ataatatttc aaatattatt
    34141 tttaggtaac aacagacatt ttttgcggct tttaggcaaa aaaaaaccaa aaatagaaca
    34201 ttctcttgaa accttacttc gtgagcttct acttttgttt ccttatatta aagccgaaac
    34261 ggacaatatt tcgtttctgc cttttcttca tcagatagtt tttgcaactt catatactac
    34321 acttattttc tataaaagtc aacacttcct ggcgtttctt ctgctttttc gcaacatttt
    34381 cttaatgttt tcaattttat gttttcaatt atctgacaat tcttaattga cagacctttt
    34441 tatctcggtt atactttaga aaattcattt tgtggaggaa cttctatgcg aaccaacatc
    34501 aaccaaatcg caccaacaca actgaataac ttttacttta gctactttag ataggtttgt
    34561 attcatcatg atggatacaa acgttaaacg acagtttgta tctatgatgg ctaaagaact
    34621 ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt ggctgagcag tgaaaataca
    34681 aagtaaaagt atcaagcatt ctactttttc aatttatttt ccgacttttc aatccatttg
    34741 gacattttgt ctgaatggat tttttgtttc ccaaaattaa ggaggaaaaa gaaatgaaaa
    34801 aaaatataat catcggatta ggcgctgccc tgctactagc tttaggaggt tgcgcacaaa
    34861 aagacaattc cgcagaggca tcagatggac aaaaaacaat tggcgtattg caacttgtac
    34921 aacataactc gttagatgct gcctataaag gattcaaaga aggattagca gaagaagggt
    34981 ataaagaagg cgataatcta acaatcgatt atcaaaatgc ccaaaacaat caagacaacc
    35041 tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga tttgctactt ggaatcgcca
    35101 ctccagcagc acaatcttta ttaaatgaaa caaccactat cccgatgact gtcacagcgg
    35161 tcacagactt ggaacaagcc catttagtca catccaatga aaaacctggc ggcaatgtga
    35221 ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg cttactgtta aatatcgtgc
    35281 cagacgccaa aacaatcggc atcatgtata acaacggtga agcaaactct aagatccaag
    35341 ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa agtcaaagta ctgacagcaa
    35401 attccacaaa tgatgtccaa caagtgacga aaagtctagc taaagatgta gatggaatct
    35461 atattcctac tgataacacg ttcgcaagtg cggcaagtgt tgtcggtgaa gtcgcaaaag
    35521 aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt caaagacggc ggtctagcaa
    35581 cagtaggaat cgactacgaa agcttgggca aacaaacagg gatcatggca gcaaaaatct
    35641 tagacggcaa gaatccaagt gaacttcctg tcgaaacagc gaatgatttg gaactgtatg
    35701 tcaataaaga catggccaaa gcattaggca tcaatccaga cagcatcaaa gaagctgaat
    35761 aaaacacagg aggattctca tgacaaattt gctctctctt ttcttatcat ctgcatcgca
    35821 aggggtctta tgggccctac ttgcgattgg tgtatacgtc actttccgta tcttggacat
    35881 tgccgacttg actgctgaag gaagctttcc tttaggtgca gggatcgcgg cagtaagtat
    35941 tacaaatggc tatcatccat tgacagcttg tcttctaggc tttttagggg gtgcggcagc
    36001 agggcttgtt tccggactgc ttcacacaaa attgaaaatt cccgcccttc ttgcagggat
    36061 catcaccatg actggtttat attctgtcac atcccgtgtg atgggtgcgc cgaacatctc
    36121 tttattgggt cagccaacga tctttcattg ggtcgagtca ttcggcataa caaaagacaa
    36181 cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt gtatgtttac ttgttttgtt
    36241 cttacgtaca gaaacaggac ttgctattcg tgcaactggc gataatctgg caatgagcga
    36301 agcaaacggg atcaacacag ataatatgaa gatcatcggt tatatgattt ctaatggatg
    36361 cattgctctt tctggctcct tacttgcgca aaacaacggc tttgctgatt taaattctgg
    36421 tattggaacg atcgtaattg gacttgcttc tattatcatc gcagaagttt tgttacgtaa
    36481 ccagccacta tttctccgtc tcttgactgt cgttttaggt gcggtcattt atcgttttat
    36541 tttagcgtta gtcttcgaac tgaatgttga accaagtgat tcgaaacttg cttctgcttt
    36601 cgtgctagtg atctgtcttt ctttcccgca gatccagcaa aaattccatt tacctcaaaa
    36661 aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat tggaaaatct ccatcatacc
    36721 tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag gcattaatct ttccttagat
    36781 cccggcgact tcgtgacgat catcggtgga aatggggctg gtaaatctac tttgctgaac
    36841 agcatcgccg gtacattttc tgtcgatcag ggtcaaattt tattgaatgg aaaaaatatc
    36901 acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc gcgtattcca agatcctaaa
    36961 ctagggacag ccgttcgttt aacggtagaa gaaaatcttg ctcttgctat gaaacgagga
    37021 aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc gaacattttt caaagaccag
    37081 ctggttcggc taaatttggg gttggaaaat cgtttatcct cagaaatcgg tttgctgtct
    37141 ggaggacaac gtcaagctat tacgttattg atggccacat tacagcgtcc agaactgatc
    37201 ttattagatg aacacactgc agcgcttgac cctgctactt ccaaaacggt gatggaattg
    37261 acagaaaagc tgatcactga gcagaatctc actgctttta tggtcactca tgacatggaa
    37321 gacgcgatcc gctatgggag tcgactgatc atgctgcatc aaggaaggat cgtcgtagat
    37381 atccagcaag aggaaaaaca gcagctcaca gtgccccaat tattagcgtt gttcaaacaa
    37441 aacagcggca cggcattaaa agacgatcaa gtacttttat cttcataatc agaaagagga
    37501 tgggacaaaa aatgaacgcc caccctcttc ttttttctct ttccttcttt cgtttttgct
    37561 ttatgtatca accgttgttt cttcgaaaac tattttgttc tctccttctt cctgatagaa
    37621 tggatgggaa aggagcttga tgccatgaca acgaaagaca aggtacttca attattgaaa
    37681 aagacaccag attttctttc cggagaaaag ttggcacagc aactggaagt ttcccggaca
    37741 agtatttgga aagccatcaa agaattggaa aaggcaggct atcgttttga gcacgggcct
    37801 acagggtatc gttacctgcc ctctgacgta ctagatgcaa aagaaatcca gcaagagcta
    37861 cggtcgttta ttcctgattt gaaagtacaa acgatgttga cttctgaatc tacgatgaaa
    37921 gatgccaaac tggcagcagc tcaaggagat tctgtttcga agttatttgt tgctgataca
    37981 caagaacaag ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg acgaggaatc
    38041 tacatgagtc tcttgctcca tccagacaaa cggtttgatg aattgcctca atatacgatc
    38101 atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc tgcttcgatc
    38161 aaatgggtca atgatatcta catggatgga aaaaaagtct gtgggatttt atctgaagca
    38221 gtcagtgata tggaaagcgg caggatcagt catatcatca ttgggatcgg gcttaatttt
    38281 tctgttcctc aaaatcattt tccagctgat ctccaagaaa aagccatctc tgtctttccc
    38341 gacggacaag ctagtgtcac aaggaatcag ctaatcagca aaatctggca agaattgttc
    38401 acttatcttt ctcacccaga aaaagcgttg gacttctata aagaaaaatc attcgtattg
    38461 ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc aaaagaaatc
    38521 ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga agatgagttt gtcttctggc
    38581 gaaatcagtc tcacatctat ccgtacgcaa taattatcag atttcacaaa aggacacctg
    38641 atctcataca gtgtcctttt ttctgaattc tcttcaaaag actctcctta ttctatcagc
    38701 tgacagtttc tctatcctct agaaaaaggt attctaaaaa tatctataaa gcatggtaca
    38761 ctttctttaa atgaatcaat gcaaaaaaca agagagtcat cagtttacac gatcaaaaga
    38821 gatagcaggc cgcttccttt ttcaatctta tagatagacc atgggtaacg ataaaaacaa
    38881 aaaagaactt gcgacttatt ttgccgtaag ttctttttta gctagtgcct taatctctta
    38941 atcccaacaa acgtctcgca tagatcacct gaccagagtg catagcagca tcatcaataa
    39001 tagaaacgag acgcattcct cgtgtgactg gtggtgtcca gttctcgtca attacttcat
    39061 ctagctcgga aggttccagc cttcttaagt aagaaatagc aaaatcagca gctcctcgca
    39121 agtatcctag cagatcttct atttgattag cttggatctg ctttgcttgt tccagactat
    39181 gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc cagtcttgac
    39241 tatgccaaat tggagaagtt cctgctaaat cagcaatctg gaaatctaat tctcgagcgg
    39301 tatgccaagc cagccaagtc attgatttga tctgcggggc tctatcttcc actggaaaac
    39361 gattggcctc ctcaatagag acgccttcaa acaaacgttc aaaccgatcg tatgcccgat
    39421 ccacactctc gcatagcata tctaaataat ccatttattt tcctcctttt tatttatcct
    39481 acttactttt tcctaattct aggataccta cttatatgtt actacacacc cttatttttt
    39541 acactttaac agtcaccatc atcaatagtt tggagattga aagaaaaaga tagtgtcgaa
    39601 ctatcaattg aacaagaagc aacaatatga acgtttcatt aatattcttt aaataaacaa
    39661 tgatttttca aacgaacagt ttaagactag gagaaaaaga gtataatagg aaaatgtttg
    39721 aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt actagtccta gttgctttgt
    39781 tgttcaatat cttttcgtat ggtgtggccg tttcagcgga aacatacatt cctaaagacc
    39841 ctgggaatat ttataatgat ctaactggta actatggctt gttaggaatt gctagtcaat
    39901 tccacgtatt tgctaaaaat aagacaactg tgaatgcaca tacagacgga aatatagcaa
    39961 cacatgaact tgatgcaaaa aataattttg gtacagagct tcatgaaggt ttattgaacc
    40021 aagaaataaa ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc atacctacag
    40081 gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg
    40141 gtcatcctac agtcaatgga tccaggcttg atcacttaac tccagatgat ttttataaag
    40201 atcgtgctgg gcatgaatat attgatttcg agcaggaatt tgctaaattg aatgtagcag
    40261 caaatgatct tgcaacgatc actccagcta aaacatatac tgctgcagat tttccagaca
    40321 tgaacaatcg gacaatcgat ttaactgatt tatcagatac cgggttttta ttggtaaata
    40381 tcgatgcaga agtgttaacg atgaatacgc cacttcaaat cattaatcca aatgatcaag
    40441 tagttgtatt taatgtcatc aacagttctt ctgcattgaa cgtacagtca ccaatcaaat
    40501 acaatgatcg ttcaaatcat gaaacagaag atttttcgga tgcgaatatc tcttggaatt
    40561 ttggaaatga gatgactgat ttgacaatca gtgctccttt tcaaggaaca atccttgcgc
    40621 ctaatgctac tatccgcatt actcaaaacc aagacggaac gattatagga acaaatgtga
    40681 ttttagatgc agctaccaat cgttgggatc ctaacgaaat atttatcagc gacaatggta
    40741 cagatacgac agacacaagc gacagtacag atacatctac cacaagtgat agctcagatt
    40801 catcaacttc aagcgacagt acagatacta cctctaccac aagtgatagc acggatacat
    40861 cagcttcaag cgacagtaca gatactacct ctaccacaag tgatagcacg gacacatcag
    40921 cttcaagtga cagtacagat actacctcta ccacaagtga cagcacggac acgtcagctt
    40981 caagcgacag tacagatact acctctacca caagtgatag cacggacaca tcagcttcaa
    41041 gtgacagtac agatactacc tctaccacaa gtgatagcac ggacacgtca gcttcaagcg
    41101 acagtacgga tacatctacc acaacaagtg atagcacgga cacgtcagct tcaagtgaca
    41161 gtacagatac tacctctacc acaagtgata gctcagattc atctaccact acaagtgata
    41221 gcacggacac gtcagcttca agtgacagta cagatacatc ctctaccaca agtgatagct
    41281 cagattcatc aacttcaagc gatagtacag acacgtcgtc taccacaagc gatacacgtc
    41341 atgcaagtgt ctccttctca aaaacaagtt cggataacag gacgaacttt aacttaacta
    41401 ataaaacaag caatgacaat ggaaacaaat caaaaagagc acttcctaaa actggaagtc
    41461 aatcgaataa ttggataaca ctcgcaggcg ttatattact tgtaatcggt ttaagaatca
    41521 cttttatgag ttataagaaa tcgaaaaggt aaaaaaatat tcaagataaa aaagagcaac
    41581 gtcgctaacg gacgttgctc tttatttttg ctttttatca taaatacaag aactacaatt
    41641 caatttgtca aaaaacacgt agatttcctt ctgagaaatt tatttacgta tcgaaccttt
    41701 ccctattcag tctttgcgat ccaaaatcag ttgggtaagg gaaaagagat tctcttttgt
    41761 tcgaagagag gtggcaggta atttctttat ttccttcaaa gccttttctg tgtaagaggc
    41821 cgctaacttc ctagcttctt ccacgcctcc tgtatgttca acgattttgc gtacggcgtc
    41881 gacatcttct tctgtcattg ccgctttctt ctctaaataa ggtagtagct ttggtttgtt
    41941 tttttgcaaa gagtagatca acggtaaaga atagacccct tgacgcatgt cttctagtac
    42001 aggtttgccg atttcctggc tctcttttgt ataatctaaa atatcatcga taatctgaaa
    42061 ggccatcccg atagaaagtc cgatatctcc tgcttttttt gcgaagcgct cgcttgttcc
    42121 actttcaaaa gcgccaacag aacaacttaa ttggaacagt tcagctgtct tacctgaaat
    42181 attttccaaa tattctttta cagaaagttc ataattatag cgatcatcca tctgccctaa
    42241 ttcgccattc aatacttttt ccatactgcg agaattcaat tggagacttt tcaaggaagt
    42301 ggagtaatca gacaatagtt taaaacagca gacaaataaa tagtctcctg catagacaga
    42361 aacgacatta ccaaactgac tacggatatt aggctgacca cgtcgcagat ccgcttcatc
    42421 aacgatatca tcatggatca gtgtggccgt atggagcatt tcgatagaag ctgccaaagc
    42481 gatggctttg tctcgatctt gctctggtcc aaattgtgag aacaatagct gataggcagg
    42541 acgcaacagc tttccgccgg catggatcgt ttctttgatg gcttgttcga caggtttgtt
    42601 tttcaggcgg acagtttctt ccatccgttt tagtgtttct gtcaattctt tacttaaagc
    42661 aggatagttt tcccacattg gatgtagctt cataaaaaaa ctccttgtgt gacgattatt
    42721 ttttggtaaa cattgggctc catttttgtt tggttccctg ctcataatct ttttggaatc
    42781 gttgaagtgt ctgcacatgt cgtaaaagat aattcgctgc gatcccgatt gcaatccctg
    42841 ataaaatccc taaaactgaa aggactggca agtacaacat gactgtccat gattgtgcaa
    42901 tgaaagaagc catcaaaagc tgtccgacat tatgcatgaa accgccggcc gcactaattc
    42961 cgatgatact gacacgtttt ggtcctaatt gcttgaccaa taacattcca aaataactaa
    43021 gcaaagcacc actcatacta taaaggaatg tggaaatcgt tccgcctagc aaagtagtca
    43081 ggaatagtcg tagccaaagt agtaagaagc tgtcttgttt tttcatggta aaaatggcaa
    43141 cgatcgtgat tagattggct aagcctagtt ttgcaccagg cgcaaaagaa aaaggataag
    43201 ggatcatatt ttccaataaa ccaatgatca ctccttgtgc aacaagtagc gaaatataaa
    43261 tcattttttg taatttactc atggtgtgtg aaggtctaat aaatcagact gtcttcgtca
    43321 cctccatctg ttgaacggac ttcgatcact aatttatgtg gcaaacagac gatggtttcg
    43381 ccatttttcg ttgcccagcc tcgacgtacg cagatctggt catcgcaatc tgcttctttt
    43441 atccgtactc gatcgccatc tatctcgatc aagttataat ctccgtgttc atctttgtaa
    43501 gtatatgtgt tgctctcttc tccagccatc aaagggaatg ttttgatttc tgtgccatcc
    43561 acacgcagta ccgcttcttt ttctgctgtt gcggtgtttt gctgaagacc aaaaatcatg
    43621 actggtaaaa aagaaagaac agtcaaaagc aaaataataa aaacatccca aggtttgagc
    43681 agactttttt cagcaaattc tttaaggctc atttttgttc tcctttttcc agattaactc
    43741 acccaatttt agcataaaaa caagaggttg tgacacctgt gtcttgcttt gagtaataag
    43801 aacgaagaat agaaaacagc tttccatgtt ttctaattct ttgggcttat taccgagaag
    43861 cagcaggtgg aacaccgttt ataacaagag gttgtgacag aagtgggcaa cttcaagaaa
    43921 taagaaggaa tttacgaaaa ttacttttca agtttttgtg aaatttcggc ttatttttcg
    43981 aagaagctac ttctggaaca ccgtttataa caagaggttg taacagaggt tgtgacacct
    44041 gtgtcttgct catggaggct tatctcgggc atcttattcc tttaatttta cataaaaagg
    44101 aggctgtgac ggtttgtctc agcccctttc tattgaaatc ttgcattcaa ataacgttct
    44161 ttatctattt atttcttgcc gtactgggaa cttggtctac cgtaccacca attacctagt
    44221 gtacgttgta gccatggaat tacccagcga tccaacccaa tcgcacgtcc ggaaccgttc
    44281 atcaatgcga aagcaacaaa aatgaaccaa atgtttaccc agtaaaacat gcctgataag
    44341 cagaacgtga tcaccaatac aatcgttgtc gcactgcata gccatgtgaa taatccagca
    44401 atcaatgcta atgccaaggc aatttcaaag aataccatga atttttggaa gaacattgct
    44461 acatcattgt ttggcatgat cacttgcatg atctttgtga accagtctgg tgacttctca
    44521 atcacagcca ttggttcttc accgtatgca taactcaaac caaaatgtgc agcttgagtc
    44581 gctgcttctc cggcacttgc ccctgctccg ctggcagctg tggttgcatc accagcagca
    44641 gaagcaccac tggcaacatc tgttacattg tattcccatg tccaagggaa cacactgttt
    44701 ccagtgaacc atgatccttc accaaaccaa gtacttggtt tgaacacttc accattccca
    44761 atgatttttt tggaagcttc aacgaaccaa actaaaccat agaaaatacg aagtggcaca
    44821 ctccataaga cattgccgta acgagaagaa tgtcctcgag tcacgttgcg atcatctttg
    44881 atatggaaaa actcatgcat taaatattgg aacatataat aaccagaacg aatatcaaag
    44941 aagtatttca aattcacgat atgtttcatt acgatagcta aaaatccgct taaatggatc
    45001 ttatcaaata ggttagctac cccccatttg gcaccaatag aaaccataaa tccttgatag
    45061 tttcctttaa atttatgctt ttctccgcct ttgatcgaag ccacgatatt cgctgcggct
    45121 gtgtgtcctg tttgttcagc agcttgaacg atctgcggtg tcggtgttcc tgggaattct
    45181 tcataaaaga ctaaatcacc aatgatatag atgtttttat cttcgtagcc ttttgcctgc
    45241 atatattcat tggcgatcaa ccgctgtccg cgtgctgatt ctaatccaaa gtcagcagcg
    45301 tcagaagttc ctttgacacc agctgtccaa atcagtgtgt gagtcggaat gttgcgtccg
    45361 tctttcaatt tgatatgatc agcagccact tctacgattg gtgcattcaa taataattcc
    45421 acgttgtgct tacgcaaata gcgttcagct tttgcagcat catttcgatc caacatattc
    45481 aagatcgtcg gcattgcctc tacgaccatc agtgtgattt cgtttggatc taatttataa
    45541 tctttggcta ggcgattttt ccagtcgatc agttccccaa tcatttcaat accggtaaat
    45601 ccagaaccac agacaacgaa agtcagcatg gctttacgca gttcatcatc tggttccaat
    45661 gcagcttttg ctactgtctt ctcaatatgt tcccgaatcc ttacagaatc ttcaaatgac
    45721 caaagggtga atccgtgtgc tttgacacca ggtgtaccaa agtcattcgg ctctccgccc
    45781 attccaagga tcagctgatc aaaaggatag gaacctaatt tggtcttgac gatttttcgt
    45841 tctttatcga taccgattac agtgtctgtg acaagttgta cattcttttg acgagcaaac
    45901 aaatgctgca gatcgtattg gattgcttct ggttctacac gtccgccagc tacttcatgc
    45961 aactcggtca tcatagtatg gtacgaatga cgatcgatca gagtaattgc tacatcttct
    46021 tttttcagtt tctttgccaa gtattttgta gctgaaacac cggcatatcc tgctcctaca
    46081 acgacaatat tttgctttgc cattgatcat ccgctcctta tagactatct atataatgaa
    46141 tatatgataa ttcttttagt ttgatactta ttacacaatc tccatatatt atatagatgt
    46201 tctgttcttt tgtctaactc cttttggatt tttttcaaat tttaaaataa agcgtttttt
    46261 cacgaaattt gctgaaagaa aacgtttgaa atattacatg ggttatttat caaaaatagt
    46321 tgacttatag gctaaaaaga ttagaatgta taatgaatca caaagaaaaa ttgtggacta
    46381 gaaatggaat ggtggggtat taaaatgaag aaaaaacgtt ttgtaactgg atttgccgta
    46441 ttagcatttt cagctctagt gttaggcgct tgcggcgcag atgacaacgg aagctcaaac
    46501 tcttcttctg aatcatctac agcacaatct tcgactgcaa aaagcacaac agaatcatct
    46561 gcaaaagttg tagctggcgg agatttgcaa gacggcacat ataaattaga agaaaaaaat
    46621 tattcaaatg gttaccgtgc agtctttgaa atggttgtaa aagacggcaa aatcactgaa
    46681 tcaaaatatg ataacgtcaa tgaaaacggc gaatcaaaaa caaaagatgc agaatacaac
    46741 aaaaacatgg aagcaaaatc tggcacaaac ccagaaaaat acattcctga gttgaatgac
    46801 caattcttaa aagcacaaag cgcaagcggt gtggaagtcg taactggagc tactcactca
    46861 tctgaaagct tccaaaacta cgcacaacaa ttgatccaag ctgctcaagc tggtaacaca
    46921 gatacaatcg aaatcgacaa tggtgctgat ttgaaagacg gcacttacaa gcttgaagaa
    46981 aagaactact ctaacggcta ccgtgtgcaa ttcgaaatga cagttgctgg cggcaaagta
    47041 actgaatcaa actttgatta tatcgacaaa gacggtaaat caaaacaaga tgatacagaa
    47101 tataatgaaa atatgaaagc aaaaagcggt acagaaccaa aaacttacat ccctactctg
    47161 aacgacgaat tcgtcaaagc aatgggcgaa gaagatggct cacctgctaa tgtagaagtt
    47221 gttacaggtg ctacacacag ctcacattca ttcatcatgt acgcacaaca attagtgaat
    47281 gcagctgaaa aaggcgacac tcaaacaatc gaagtagata atatcgtaac agaaaaataa
    47341 tcacataatc atactaataa aagttggagg gtgggacgat agccagatta ggcagtcgtc
    47401 tcacctttca aaatgaagag aggaaaaaga tgcggaaaaa actactattt tccttaatca
    47461 cactgggcat ctcttttatg gtgctaactg gctgcagcaa ctcagcaaat aaaacaacca
    47521 ctgaaagttc agcagctacc aagatattga aagatccata ttctgatgaa cagttcttgt
    47581 tagggactta cgttcgtatc cgtgtgtatg atgaaggcaa agaatcagca ttgaaacctg
    47641 ccttcgatcg agtaaaagaa cttggtgata aaatcacgat caatcaaaaa ggttctgaga
    47701 tcgatgaagt gaatgagcaa gctgggatca aaccagtaaa agtttctgat gacgtctaca
    47761 cgttagtcaa gcgggcctac gaatatagcc aagattctca aggcggattc gatatggcga
    47821 ttggtgcaat cacacaatta tggcgaatcg gttttgatga cgccagaaaa ccttcccaag
    47881 aagaaatcga tcaggcattg aaattagtgg attatcataa gatcgaacta aatgacaaag
    47941 aaaaaactgt ttatctaaaa gaaaaaggaa tgatcatcga tttaggtgca atcgccaaag
    48001 gttacattac ggacgaagta gtgaaagtct tgaaaaaaca aggagtgacc actgccatcg
    48061 ttgacttggg cggtaatgtc tatgtcttag gccacagccc aagaggcgaa aaccaagact
    48121 ggacagtcgg catccaagat ccgaacatgg ctcgcggatc tgttttaggt tctattaaag
    48181 aacggaacaa aacattagtt acctctggaa tttacgagcg ttatttagaa gtagatggca
    48241 aaaaatacca tcatttgttt gatccaaaaa cgggttatcc gttcgacaat gatatcgcaa
    48301 gtgtcacgat cataacagat aaatcaattg atggtgacgg gttgtctaca gcagtctttt
    48361 caatgggtgt gaaaaaagga ctggaatacg tcgaaagcga attgaataac gggacggaag
    48421 cgatcttcgt gacaaaagac gataaagtat atgtgaccga tccaatcaaa gacaccttca
    48481 aattatcgga ggactcccat tacacgatgg gtgaccgttc tgaactaaaa taaggagtac
    48541 gaaccatgac actaaaaatc tttttagaag tagttgaaat acgaacaaag gtggctagtg
    48601 tatttccttt tgcggtaggg gtattattct ctatcgcttt ttttcatgag atacattggc
    48661 taaacacttt tttatttttt attggaatgc tagtttttga tttagcgact accgctatca
    48721 ataa
//
LOCUS       DACDTH010000013        48395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDTH010000013 DACDTH010000000
VERSION     DACDTH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.013"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            277..1602
                     /locus_tag="IWK54_000821"
     CDS             277..1602
                     /locus_tag="IWK54_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9592964.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            1595..1945
                     /locus_tag="IWK54_000822"
     CDS             1595..1945
                     /locus_tag="IWK54_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592965.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            1942..2121
                     /locus_tag="IWK54_000823"
     CDS             1942..2121
                     /locus_tag="IWK54_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592966.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            2257..2547
                     /locus_tag="IWK54_000824"
     CDS             2257..2547
                     /locus_tag="IWK54_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9592967.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            2915..3703
                     /locus_tag="IWK54_000825"
     CDS             2915..3703
                     /locus_tag="IWK54_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9592968.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            3690..4016
                     /locus_tag="IWK54_000826"
     CDS             3690..4016
                     /locus_tag="IWK54_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592969.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            4365..5405
                     /locus_tag="IWK54_000827"
     CDS             4365..5405
                     /locus_tag="IWK54_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9592970.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            6241..6573
                     /locus_tag="IWK54_000828"
     CDS             6241..6573
                     /locus_tag="IWK54_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9592971.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            7164..7493
                     /locus_tag="IWK54_000829"
     CDS             7164..7493
                     /locus_tag="IWK54_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592972.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            7480..7821
                     /locus_tag="IWK54_000830"
     CDS             7480..7821
                     /locus_tag="IWK54_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9592973.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            7935..8174
                     /locus_tag="IWK54_000831"
     CDS             7935..8174
                     /locus_tag="IWK54_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592974.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            8241..8480
                     /locus_tag="IWK54_000832"
     CDS             8241..8480
                     /locus_tag="IWK54_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592975.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            8566..9237
                     /locus_tag="IWK54_000833"
     CDS             8566..9237
                     /locus_tag="IWK54_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9592976.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            9290..11266
                     /locus_tag="IWK54_000834"
     CDS             9290..11266
                     /locus_tag="IWK54_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9592977.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            11279..11935
                     /locus_tag="IWK54_000835"
     CDS             11279..11935
                     /locus_tag="IWK54_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9592978.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            12031..12858
                     /locus_tag="IWK54_000836"
     CDS             12031..12858
                     /locus_tag="IWK54_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592979.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            12868..13116
                     /locus_tag="IWK54_000837"
     CDS             12868..13116
                     /locus_tag="IWK54_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592980.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            13147..15258
                     /locus_tag="IWK54_000838"
     CDS             13147..15258
                     /locus_tag="IWK54_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9592981.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(15296..17353)
                     /locus_tag="IWK54_000839"
     CDS             complement(15296..17353)
                     /locus_tag="IWK54_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP9592982.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(17467..17679)
                     /locus_tag="IWK54_000840"
     CDS             complement(17467..17679)
                     /locus_tag="IWK54_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592983.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            17866..18333
                     /locus_tag="IWK54_000841"
     CDS             17866..18333
                     /locus_tag="IWK54_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592984.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            18330..18572
                     /locus_tag="IWK54_000842"
     CDS             18330..18572
                     /locus_tag="IWK54_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592985.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            18590..18892
                     /locus_tag="IWK54_000843"
     CDS             18590..18892
                     /locus_tag="IWK54_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592986.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            18963..21017
                     /locus_tag="IWK54_000844"
     CDS             18963..21017
                     /locus_tag="IWK54_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592987.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            21017..22849
                     /locus_tag="IWK54_000845"
     CDS             21017..22849
                     /locus_tag="IWK54_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9592988.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            23544..25460
                     /gene="ltrA"
                     /locus_tag="IWK54_000846"
     CDS             23544..25460
                     /gene="ltrA"
                     /locus_tag="IWK54_000846"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9592989.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            25577..26377
                     /locus_tag="IWK54_000847"
                     /pseudo
     CDS             25577..26377
                     /locus_tag="IWK54_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            26374..28905
                     /locus_tag="IWK54_000848"
     CDS             26374..28905
                     /locus_tag="IWK54_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592990.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            28955..29287
                     /locus_tag="IWK54_000849"
     CDS             28955..29287
                     /locus_tag="IWK54_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592991.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            29288..29881
                     /locus_tag="IWK54_000850"
     CDS             29288..29881
                     /locus_tag="IWK54_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592992.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            30044..31738
                     /locus_tag="IWK54_000851"
     CDS             30044..31738
                     /locus_tag="IWK54_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9592993.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            32426..34303
                     /gene="ltrA"
                     /locus_tag="IWK54_000852"
     CDS             32426..34303
                     /gene="ltrA"
                     /locus_tag="IWK54_000852"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9592994.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            34434..34811
                     /locus_tag="IWK54_000853"
                     /pseudo
     CDS             34434..34811
                     /locus_tag="IWK54_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            34811..36016
                     /locus_tag="IWK54_000854"
     CDS             34811..36016
                     /locus_tag="IWK54_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9592995.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            36032..36676
                     /locus_tag="IWK54_000855"
     CDS             36032..36676
                     /locus_tag="IWK54_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592996.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            36687..37493
                     /locus_tag="IWK54_000856"
     CDS             36687..37493
                     /locus_tag="IWK54_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592997.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            37515..37745
                     /locus_tag="IWK54_000857"
     CDS             37515..37745
                     /locus_tag="IWK54_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592998.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            38291..38566
                     /locus_tag="IWK54_000858"
     CDS             38291..38566
                     /locus_tag="IWK54_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9592999.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            38608..39030
                     /locus_tag="IWK54_000859"
     CDS             38608..39030
                     /locus_tag="IWK54_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593000.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            39049..40641
                     /locus_tag="IWK54_000860"
     CDS             39049..40641
                     /locus_tag="IWK54_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593001.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            40653..40988
                     /locus_tag="IWK54_000861"
     CDS             40653..40988
                     /locus_tag="IWK54_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593002.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            41135..41329
                     /locus_tag="IWK54_000862"
     CDS             41135..41329
                     /locus_tag="IWK54_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593003.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            41361..43733
                     /locus_tag="IWK54_000863"
     CDS             41361..43733
                     /locus_tag="IWK54_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593004.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            43794..45254
                     /locus_tag="IWK54_000864"
     CDS             43794..45254
                     /locus_tag="IWK54_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9593005.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            45265..47469
                     /gene="topB"
                     /locus_tag="IWK54_000865"
     CDS             45265..47469
                     /gene="topB"
                     /locus_tag="IWK54_000865"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9593006.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            47715..48308
                     /locus_tag="IWK54_000866"
     CDS             47715..48308
                     /locus_tag="IWK54_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9593007.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
BASE COUNT        17929 a         7544 c         9786 g        13136 t
ORIGIN      
        1 tcaaatgttg taaggatttt attcatttgt taatattaaa gaacggaact ccaaaaaagt
       61 ccgtactcta atttgcagtc tctcactctt ttagagtgag agtttttatt agattttcaa
      121 attaattagt aaagtgatag aataaattga tcatacaaat cataagaaga agcagggtga
      181 tagcatacct atgcttcttt ttttatgtat ttagctattt cttctttaca acatcgaaca
      241 tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc aaaagagcca
      301 gtaaatgatt actttttaat cgatatgaaa agcttttacg caagtgtcga atgtatagaa
      361 agaaatttag atccattaac aacagaactt gttgttatga gtcgatctga taatactggt
      421 tcaggattga tattagcttc ttctcctgaa gcaaaaaagc ggtatggtat tacaaatgtg
      481 agtagaccac gtgacttacc acaaccattt cctaaaacac tacacgttgt tccaccacgt
      541 atgaacctat atatcaagcg aaatatgcag gtaaataata ttttcagaag atatgtggct
      601 gatgaagatc tactgattta ctcgatcgat gaatcgattt taaaagtgac aaaatcactg
      661 aatcttttta cgactgaaga aacacgaagt cagcgaagga agaagctcgc tcaaatgatc
      721 caagaacgta ttaaagaaga gttaggtttg attgctacgg taggcgtcgg cgataatccc
      781 cttttggcta aattagcctt agacaatgaa gcaaagcata atgaaggatt tattgcggaa
      841 tggacgtacg aaaacgttcc tgagaaagta tggaatattc ccgaaatgac ggacttttgg
      901 ggaattggtt ctcgaatgaa aaaaaggctc aaccaaatgg gaattttgag cattagagac
      961 cttgctaact ggaatcctta tacgattaag aatagattag gtgtgattgg attacagctg
     1021 tacttccatg ctaacggaat cgatcgaaca gatatagcaa tcccgccaga accgacaaag
     1081 gaaaaatctt atggtaattc acaagtgtta ccacgagatt atactagaag aaatgaaatc
     1141 gagctggtgg taaaagaaat ggcggaacaa gtagccattc gtatcaggca gcataattgt
     1201 aaaacaggat gtgttcattt aaacattggt acgtcgatct tagaaacgag accaggattt
     1261 tcacaccaga tgaaaatacc tatcacagat aatacaaagg aattgcagaa ttattgtctt
     1321 ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat aacctatagc
     1381 aagcttgtgt acaccgattc gcttcagctg gatttattta gtgatccaca aaagcaaatt
     1441 aacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg ttttacgtcg
     1501 attgtacatg cttcttcaat gttagaaagc gctagaagca ttactagaag cacacttgtt
     1561 ggtggtcatg cgggcggaaa tgggggaatt aaaaatgatt gattacaatg atcgtggacg
     1621 tttaaagtgg gcagggtttt atttatctga tcatactgaa gaaattgatg ccgacaatga
     1681 acaacgaacc agtcaaaatt tagcaaaaga acaaatgacg actgaagaga ttgcggaagt
     1741 attgaacgct gcagtcataa aaaatagatc agtatctatt caaaaagaag aacgaaacgc
     1801 tgaaggatac tatccagaag atattgttgg caagatctta ggtagtgatg agctggggat
     1861 ctacgttgga aaagacaaaa ttcactacga cgaaatacgt catatcgaat tttaccagga
     1921 taataaatgg agtcgattag aatgaacatt atccagcaat atgagctaaa gtatataacc
     1981 tttgatcagc tatcagaaga gatttggggt tatggccaac gtttgataaa tgaggttggt
     2041 gttgagcgct tctctttcta tgtagaagct gcagcaggtt atcataattt taggttttat
     2101 attttcccgt tatatatata aggtatttta cggattctat agagttatct ctatgaatcc
     2161 ttttttgttt ttacaaatag gagtagatac tagaataatg cgtgtgttaa actattattg
     2221 caaacgcaat aataaaagaa agtaggctta cgcaccatgt ctatcaatac atttgtttac
     2281 tcgcacccga tcaatgttta cattatcaag aatttaggaa tcactgtaga acaattttgt
     2341 gagttatatg cctacccaca aggaacggtt gcaagctgga taacccgaca acgtagaata
     2401 aaatcattac ctgcttcatt cgtttatgat ttaagcctgg cctctagttt aaacatgtct
     2461 gatgtttatg aaaagctcct gtctttagaa aaagaatatg attccttcaa gattaaacat
     2521 gataaaaaaa taaaaaaaca tatataatat caatcacaat tgtataatat tacgatatct
     2581 taatatcatg atatcgtaat attgtttttt tctaagaaaa tgcactataa gaagggaatt
     2641 attagtaaca atatagacag tttaataatg tttaaattat tctagttgct gctcgcaaaa
     2701 catatttctt atgattaata aaaaatttta aagtaataaa aagtgattat tttactttcg
     2761 aataacatat gaataatctt tatatccgaa ccgatatata tctttaaaac aaaataatca
     2821 cacaatatta caatataatg catatacgat attgcaatat cacaatatta tgatataatt
     2881 atattataat ataagatgaa tgggggatat tttaatggca agaatattgt cagtagccaa
     2941 tcaaaaaggt ggagtttcta aaactacgac tagcgttaat ttagcagcat gtttgaaatt
     3001 tgtttacggt aaaaatgtac ttttagtgga cattgatcct cagggaaatg ctacagataa
     3061 tgtaggtttc gatatagatg gaacaaacca acctacaatt tatgaagtat taaaagggga
     3121 ggctgacatt acagatgcta ttctggacta taaaggaatt gatgttttgc cagccgacat
     3181 agccctttct tctgcagaga gggaatttac tcaagtagga agtgaacaca ggcttaaaag
     3241 agtacttcaa ccaatcaagg acaactatga ttatattata attgattgtc caccaagttt
     3301 aggaatgctt acagtcaacg catttactgt ttctgatgaa attatcattc cagttgaagc
     3361 tgcgtatttc tcattaaaag gactaataaa gttaagtgaa actattgaaa cggtaaaaga
     3421 atatacgaac gacaagcttg aagtacgagg agtcctattt actaagtata ataagcgtta
     3481 taacatttct aaagaaatga cgactgcagc aaccgaaatt tctagagtca ttggtgcaaa
     3541 aattttcaaa acttatattc gtagaacaat aaccgttgac gaagcgcaag ctgctggatc
     3601 agatctaatc aattttaata aaccctcaac agctgaagat gactataaag catttacaga
     3661 agaatattta aaagaagttg gtgattttga tggcagaaga taatcgcagt caagctgcag
     3721 taagtagact actaggaaat aaaaataaaa agaactacaa accaaaagaa gtagaagaaa
     3781 tcgaattaac caagaaaaat ataagtaaag tcataaaaga tcaaggaagt agtgtaagaa
     3841 cagcagatag cattcgtata aatccaattc taatgtcagc actcaaatat tggacgacta
     3901 ttgctgaatc agacaaaagt aaaccagacg ttatagaaga tgcgctatta aaatccatac
     3961 ctgaagaata cttgattgaa ggatataaac tagcaaagaa acaaaataaa atttaattta
     4021 cattcaatat cacgatatca taatatcgtg atattttttt tataaaaatc agtagtatca
     4081 aagctgataa gtcgcttatc ttcatattgt aatattacga tatcgtgata taatgttatt
     4141 gtaatatcgt gatatagtta ccatgagctt aaaccctgaa atagagcggt ttgctttaat
     4201 ttatttttta aggtaaacta aaaaaagata aaaaaagact actgctttaa caaagtttgg
     4261 cgacttatca gcagtagaaa aatatatagt ggatagatat atttgtttaa ttcattgtaa
     4321 catagttttg aacaaaacgc tatcctacag aaagaggatt ggctatgaat gattttaatt
     4381 attacaaatc aaaagaaatt tatcgtgaaa aatattatca aatgcctaag gtatttttta
     4441 ctaacgaaaa atacatggat ctttcaaatg atgcaaaaat tgcttatatg ctacttaaag
     4501 atcgatttga ttattcagtc aaaaataact gggtagattc tgatgataat atattcttca
     4561 tattcacagt tgaagaatta atgaaattgt tacaatgccg cgaaggtaaa gtatctaaga
     4621 tcaaaaaaga attagaaaaa gcaggtcttc tgaaacagaa gagaggaaga gtaaataaac
     4681 gtgatggtaa gattgaatct atgcctaatt tattgtactt aggcaagcca gaagttacga
     4741 atcaagacgt ctttaaaatc atggaagagg aagacaatac tgattccacc gtaattgcga
     4801 aaatcgcaaa tacggcaaaa ccgcgaaaaa tcaatgattc taccgtaatt gcgaaaatcg
     4861 caaatacggc aaaatccagt catatcaagg actcaaccgt aattgcgaaa atcgcagaca
     4921 atctattcta ttctaattct atagacacta atagacacat aatagacact gaaactgacc
     4981 agttacaaaa tcaagttttg ctagataact tcgtcgacat catgcaagag gacagtataa
     5041 atacatttgt accagaaaat gtattaaact tgataaaaac attttcttca agttacagtg
     5101 aagctcaaca aaccgtccaa actattcata atgcaaagaa aaaagctgaa gaattagagc
     5161 aaactattgt cgtttatgaa gaactggaat cttatggaat tgatgctgac aaaggattat
     5221 atatgacttt actgaaagct tatcaaaaac aaaaaactga aaaagtaaac aatttgcaga
     5281 atctaatttt cgtttatgta aaaaattggt ttgtcgaaaa agctattgcg gcaaagcttg
     5341 caaacgaaca aagagaaaca tttaacgaat taccaacagt tagtacggaa aattggttag
     5401 agtagttgat aaaaagcaat tactataaaa atgatcaatt ttaaattaga acttttttat
     5461 gattttaatt ttattacaaa tattacaaat aaatagttag tattccggag tttttagatt
     5521 aaaagtttat gaaaacaatc acaatcgtag gttttaaatt cataatcgtt agatatttta
     5581 gttatttttc actcaaaaaa atagggaact agtgtttgca tatgtgcttt tttgagttat
     5641 aataattcat gaagagaggt aattcactta tttccccaag ctgaaaagcc tgatattact
     5701 tcttttcagg cagtgctgct aatattcaat tacacttttt tgtatatttt tagttgtttt
     5761 aaatttaatt gtctgctatg cttgtgatga attgattttc gttctgcact tttggacgat
     5821 aaatttggac aattgaaaat acaaaaaaaa gaaaggccct gtgagactcg gaaggctggt
     5881 aaccttcgtc gacgtccaca ggtggcatta agatcactcc cttaatgcgt ttacctttca
     5941 gtgttacggt attacccgta acggatttag tataacagat attcaaaagt gataccactt
     6001 tttttggctg tgttaaggtt aaaaatacgt gatattacga tgaatctatg cctaagcata
     6061 atgttataaa aatgtccgtg tggctctttg ccgtatgacg aacgctgaaa ttagagggaa
     6121 cgcatgtttg gatgcgttcc ctcttttttg tttgtcatga acgaaaatca attctctatt
     6181 tttgaaaaat aacaagaaaa acttgttaaa aatatttaaa gtaagtgggt ggattatttt
     6241 atgtcatata caggaatttt gagtctcgaa gatatttgtc actacggtaa acggtgtacc
     6301 gctacagaaa agatcacgaa gaaactatca actggtcaaa gtaaaaccgt tgtgcagtgt
     6361 aagaaatata taatccagaa agacaaagtc tcagaagaaa tgatttatta cattgggaaa
     6421 caaaaacaaa taattttaaa agatcctatc cctttaaaag agctatatcc aacaatcaaa
     6481 catgtttatg accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt cttgcgttgt
     6541 accgcaaaag ggatggggcg tttaatcagc tgaattgatc attctcataa cttgtatttt
     6601 atcgtttttc gcttgcttga aaatattgtc gagacttcta aaggaactta gaatcactag
     6661 aaaaataagg agtagacttg atctatcaac aattcctttc ggggggtgac ggggtaggtt
     6721 tttacgattg gtaacgtaaa aaattggtca atcgagtaaa tgcccttgtt tggatagtgg
     6781 tgaaatgaat ttgtgtaaat aatatgcaca gagtaaacaa gagggaattt agaagttaga
     6841 caagttagaa tagcgcctag attaatcatg agcagctact aagggatcta ggctttgacg
     6901 caaactcatg gaataaggag taggtcttct cgtaatgggg aggacgcaca agaaattgtg
     6961 tcgtgtgctg cagtacatgt agatacttgc gaagacgttg atctttggat caatcgagca
     7021 tttaccgttt tctactaggg tttctcgttt cggacgccat aaacgagtcg atttatttta
     7081 tatatttttg agtgaacgaa aaacgataaa aatattcgtt atgaagtaaa actaatttag
     7141 tagatagcaa aggtggtaga ggaatgattc aaaaatttgc gaaaatgagt aaaactgcat
     7201 ggattgaaaa gcttgaaaat gacttgagtg tcatgcgatt agctccaaag aaattcaaac
     7261 gagagattgc agaaaaagaa gcaattttgt cggtattgaa tgcggtagac gataaatgtc
     7321 cagtaaaaat tagtttttca gatatagcaa caattatcga aggtatttat tatggtgaag
     7381 ttgatgaaga gatatcttct acaaaagatt tattagattt tttggataaa aacaaagata
     7441 cctttatagt actaactgat tatgaggagg ttacaacaaa tgaaaaatta tgatccgaat
     7501 agccgatggg gaacacatac gattaaggtt tctttccaac gttgggatta caaaggattt
     7561 gtgacgttta gaagaggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat
     7621 gatttatacg atcaaaaatt gactgataat ccaattggat tcggcttgct acctgaagat
     7681 gatcaaggta atgaatggtt taaaatgacg cttatgaatg ataatggaga cgaattatct
     7741 gttgaagata cttggtcata tttgagtgat tatattgtca gcgttgaaat cattgaattt
     7801 gtagcggata aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag
     7861 gagtcacgac gattaataaa aaggtagtat taccttaggc attgagtaag gaatttattt
     7921 aggaggaaaa ttagatggcg cgaccagttt tagaggcgat ggatatggat actttgacaa
     7981 aaggaaggta tgtacagctg ttagctcgtg gttattcacc taaagaagta ttcaaatccc
     8041 tgtctaaagg aacagatatg gaaaaagaaa gattacgaaa agaattcgat tattggagaa
     8101 cccataatgg aatcaaggat ttgaaaccag cacggccagt aattaaacga aagaaaaaga
     8161 taacccaaga ctgaagtgta gagcgtatgt gatcataggc ttttttattt ttgcaaaaaa
     8221 caaggaaggt aggaaaactc atgaattatg aagaaatcga aaacagaaaa aaagtgtcga
     8281 aagaaatgga agaaaagttg ctaaaaacga tgaaacaaaa acaccttaaa agattatctg
     8341 tcatgcaata tatcaatgat atgcagataa caggaaaaga gaaagcttgt ttgttaggtt
     8401 caatgaaaaa ctttgaacag ttgagacgaa cttatgtgaa aaaaagttct aattgtcaac
     8461 tattgttaga agtctcatga cattcattaa tagaattcat ttttaaaact ttttctatgg
     8521 aaatgagaaa aagatttttt gttttgtctg aaagggggta gctggatgag aaaatggtta
     8581 tttggttttt taggagtcgc tttaatcgtc ttttgcagtg tgtttggcta tgttagctac
     8641 caaaaacatg aaggaaaggt gtttaagcaa aatatagaaa aaaagatgcc cgtagatcaa
     8701 atcaatgaac atgcaaagag ttataaggag gatgcaacga acgtcaataa tgatatgtct
     8761 ttggggcaaa tgttgtccat acaaaaagaa gcaatcgaaa tgggtgtcaa taaacaagcc
     8821 tttgcacaaa ttcagatacc tgcacttggt ttagcattgc caatttttaa aggggcaaat
     8881 caatacactt tgagtcttgg agcggctact tatttttatg aagatgccga aatgggcaaa
     8941 ggaaactatg tcctagcagg tcataacatg gaaatgccag gtgttttatt ttctgatatt
     9001 caaaagttgt cattagggga ggtgatggac ttagttagta acgatggcgt ctatcgttac
     9061 aaggtaacgc gtaaatttat cgtacctgag tactttaaat taatcgatgg cgttcctgaa
     9121 gagaactctt tcttgtcttt accaaagaaa ggagagaaac ccttattaac gttgtttact
     9181 tgtgtttata cgtcacaagg aaaagaaaga tacgtggtac agggagagct acaataacaa
     9241 ggttttaatc aacgcgagtc gttgaaatat gaaatgagga gagtaaaaaa tggcaataaa
     9301 tcggtatgta aaaaaagctg gaatgcttgt tggaatgtta ggaatcattg caagctgctt
     9361 tcaggggtat caagtacacg cagaagatgt cacgcaaaaa acaccaccag agaaggtcaa
     9421 tatcacggtt cataaattaa tgtacgatca aggaacgcaa ttgaatgttg atattgacgg
     9481 gatcaaaaac gatggttata cgcacgatca atatcctaca ggtgtaacga aatacaacaa
     9541 ggcagattat ggtgatgttg agtttacgtt aggaaatatt acggatcaag tattaccgag
     9601 agaagattct gatctaacca acgcgaaagt ggacgagatt gtacaagatg tagaagacaa
     9661 gggatctaac agtgagtacg tcaagaatgc aaccaacaaa attacttcag ctgtagatga
     9721 aaatggtgag atcactttta ctgatcaacc agcctatgtc aattccaaag ggaatgtata
     9781 tgtggtttat gaaagtaaat ccgctgctgg attagtcaca caaaaagcaa aaccaatggt
     9841 tgtgattgcg ccaatgacag ataaaacagg atcaggattt ttaaaaaata ttcatttgta
     9901 tcctaagaac attgtttcta agttgagttt tgaattaacg aaatttggtg acgatggcgc
     9961 agcccaaagc aaacaaaccc ttttaaaagg ggcaaaattt gagttataca agggcgaacc
    10021 tggaaaagga acaaaattgg gtgatcttgt atcggatgat caaggaaagc tgactgcgac
    10081 tgatttgact ttaggaaaat actattttgt ggaagttcct tcggaagttg tagtgggatc
    10141 agatcaagaa ccaacagcag atcaatattt attaggcgca gacgctcgca acgatgcgca
    10201 taataaatta acctttgaaa ttaccaatga cggcgtgaca agtgacttaa aagcttccta
    10261 cgtgaactat aaagcaccag tgatcgataa aacagtgaca aatggaacag gacaagaaca
    10321 ttctttccaa attggtgacg cagtgaatta tcaaggaacg attcatatcc ctacagatat
    10381 tgcaggtgga acagatggga ttacagtaaa tggtgtgaag agtgaaactt ctccttacag
    10441 cgtatttaag tggggagata cagcaggaca aggactttct tatgtcgctg ctaaagcaaa
    10501 tatcaaagta acaaataaag atggatctgt ggtattaaaa gaaaatacag attataaaat
    10561 ccaaaattca gaaaatggct ttgtgatcga ttttatcgtg aataatggcc aagtaagtga
    10621 tactgtagca agtttacacg gacaagatct tcaaatgact tataacatgt atgtaaatga
    10681 cagcgcagct gttgccaatc cattaacaaa tagcgtggat ttcgtttata acaacaatcc
    10741 atttaatcag gaagaacacc acgaaaaaac gaaagctgac gttgtgactt atggtgcaaa
    10801 attcttaaaa gtcgatagcg gactatttgg aacaggaatc aaagcaacac cgcttgaagg
    10861 cgcagaattt gctgctaaaa acgcagaagg aaaatattat ggtggattag ttgatacgga
    10921 taaagacggt gtcaaagaag cagtatgggt agacgatgta gcaaatgcag cgatcctaaa
    10981 atcagataaa gaaggtcact ttgaaatcac tggtttaact gaaggtgaat attcactgga
    11041 agaaacaaaa gcaccaactg gttaccaaaa attaacagaa gatattgcat ttaaagtaaa
    11101 caaaaactca tttaaagagg aaaatcgaat cactgttaaa aataatcaaa aagcagctat
    11161 tccattaaca ggtagtaatg gtttccaaac gtatgtattg atctcttgct tgttgctagg
    11221 agcaggggca ctttctgcag ttgtttactt taagaagaaa gcctagaagg atcagctaat
    11281 gattattcgt aagcccccaa aaagaagagt catgcacaaa ctgatgatat ttttttggtt
    11341 gctgtcggca gtagcagtct atttaattcc agctgtggaa acaaatgtag cgaaatttca
    11401 acaacagcaa agaattttag ccattgaaaa tcttgaaaaa acagattctt ctttggatgt
    11461 tgcgaaaata gagttaggcg atcctgttgg tgttcttaca attccaagta ttcctttaaa
    11521 actaccaatt tatgacggaa cttccgacaa aattttagag aatggtgtag ggattacgga
    11581 aggaactggc gatattacag gaggaaacgg taaaaatcct ttaatcgcgg ggcacagtgg
    11641 actttataaa gataatcttt tcgatgattt gccttcagta aaaaaagggc aaaaattctt
    11701 tattaaggta aatgaagaac aacatgccta tcaaatttat cgaattgaag aagttaaagc
    11761 agaagaatta caaaaaaatt atttagatta tctcatgcct gaaaaagacc aagataggat
    11821 cacgttaatg acatgcacgc caaaaggtgt taactcacac cgctttttag tgtatggtga
    11881 acgagtgtca ttcagtaaag aagatttaaa actacagaaa aaagcaaaaa aataggaaat
    11941 caatttcatt ggattggcga ttagcactgc tattcgcttt ttttgcgttc ctattatttt
    12001 tatttataaa aaaataagga ggagatcgga atgaataaga gaaaatggct gtttgtttgt
    12061 gtagcagctc tctttgcaac tgcagcttac tcacagatat cttttgctga tagcagtact
    12121 actccaacta ccagtcagac aacagatagt tcaacaactg caacaagtga cacggacagt
    12181 tcgaagaaca ctgcagaaga aacggcagat tcaagtacga gttctactga atcagcggaa
    12241 tcaacgtcag aaagttcttc agagacttca gaaaaactgc aagaagtaga agatacgcaa
    12301 gtaatcaagc aaaacattca tattcaaaaa attatttctt cgaatcgaat caacaatcaa
    12361 ggaaggcaag tgactcagca agaaggtgtc aatggtgcaa agtttattgt ctacgatatc
    12421 acgaatatct tagaagaaat gacacaagat gataatgaga aaaatcaatc ggtagaaata
    12481 attgagtcaa aattgaaaga tcgagcaaaa aaactttcat acgatcagtt gaaaaaagtg
    12541 actgaaggag aaacccaaac aattgatgat caggaaggcg tgattgattt ttcaattgaa
    12601 gtacctgcaa acaaaaaaca agcatactat atcgtcaatg aatcaagccc tgaaaacatt
    12661 tcaaatagcg aagatattgt tttattgaca cctgtttcag atgaaaaggg tgaatttttg
    12721 acggatgttt ggttatatcc taaatcaaag aaatcagagc cgaaagaaga aattaaaaaa
    12781 gttgtatcaa ctggtgtaaa gaaaaacttt tttgataatt gttgggattt tttcactcat
    12841 ttatttttta acgattagga tgaaatgatg aaaataataa aaatgttagt ttttaatcta
    12901 tcttttttag ccgcaacaat attgtgttgc ggtttagttt tttctcaatt atcaatttac
    12961 ccaagatatc aacgattgtt cgttcatttt ctaaatatcc agcatgcttc catgccacta
    13021 gaagtgatta acaagctctt aatcaatcca atttttatgg ctagtttgtt tcttcttgca
    13081 ggattggtaa cgtgggtatt aggaaaaaaa gaatgagaag cttacaatcg tttaacaaaa
    13141 taatagatgg agggcattat ggaaaataaa attcattgca ctacgggaag agtatttact
    13201 ttagtgttgt ttttgttcct tcttcttttt gcaaggggaa ctgcagtcca ggccttagaa
    13261 aaagttgatc cgagtcaaat caaagaaaat aataatctgg tactttatca tgatagtgat
    13321 tcattgagaa atatcgaagt aactgggatc aatggattta agatactaga tgagtattca
    13381 actaaaaaac aaaaagtact tgcttggcca aaagtcaaag atttgaaaag cacgtcaaaa
    13441 attgttgccc attatcaaaa ggtgggcact tatctaggaa aagaaattga ttgcgacgta
    13501 actttttcta attttgtagc gcacgctggg gttaaccaag acggattagc taaaacaatt
    13561 aatgggaaaa agtattataa ttatttgctc tttgataaaa acatttatcg aggctttttc
    13621 atgcagtcaa tcaacgttat gaatgttaaa ctagatttta agtatgcagg atcaaaaaca
    13681 aaagtagctt tgagtgaacc acagccagaa attaattcaa aaatgagatc gatgagagca
    13741 gctggtattg agttgaatga tacatttcta tctatttaca gtttaaatgg acagtatcag
    13801 caacgtgtgt cttggtataa tgacaataat gaatctgtcg gtgaacgtaa tattgatatg
    13861 agagaatttg ttgggtacga aaaaatggat cgcttacctt attttgtcac aacagataca
    13921 gcatgtgcag aatacaaagc tcctgcgtta tcaacaaaca atttaacttc aaaagtagtg
    13981 ggaggacgtg cagaaaaggc ttatagctcg aatgatcatt tcaccgatgt tgtaggagct
    14041 gatacttatc acagaagtgg tgtaacgtat acgcttcaag gcgcttcccc aacattcatg
    14101 attggcgcaa atacgaatag tatgatgttt agctttgata ctgcattgct ttggacacca
    14161 caaccatcga agcctacaaa agaagtgttt aacaaagcta atactgaaga ggcagcacac
    14221 aatattgaca aaaaagtgat tccacaagga tcagatgttt actatcatat tcaccaaaag
    14281 tttgatgcat taacagtcaa cacaatgaac aaatacaaat catttaaaat cactgatacc
    14341 tttgacagca aaaattttga tatggtatcg gatgggaaaa actatgatgg cgcattgtgg
    14401 tatgttgaag cgaatgggac ttggaaaaaa gctgatacag gaggaaaatt aactgtatca
    14461 ggtaatacag tcacttatac tgcttctgac agctttttag ctaatgctgc aaattacggt
    14521 agggactaca ttttgcagct ccatatgaga gcaaaaaatg atgcaaaagg aattgcacca
    14581 aatgtggcta ctgtaacgtt taacaatgat ccgaacaaag gaacacaaaa aacaaataaa
    14641 gtagaaaact atttctctct taaaccaaat aaaaaagtgg aacaagatgg gaaagatgtt
    14701 gatggtcgaa atgatggtaa aaaaggaacg cctacagctc ctttaaatgc aggatctgaa
    14761 gtacaatatc tagtcacaca aaaatggcat acaaaaggtg tagacattgc gctcgatcat
    14821 tacaagcaat ttagtattca agatccagtg gaagctcgtt tgacgtataa agaaggttct
    14881 gcgcgagtga tagatatgag tacaggaaaa gatattactt cagaaggtat actaacctat
    14941 gatagccatt caagaacgct gaaatgggaa gcttccgctg atttcttaaa taacaatctt
    15001 ttagatggac gagaaattca actgatattt acagctaaaa ctcctttgaa tgaagaattg
    15061 gatatcgaca accaagcaaa agtttcagta gaaagtattg agaaagcaac taatgtagta
    15121 acgattgggg taaaacctaa cttacctcag gtcattgtgc caaaaactgg atcgaatcat
    15181 ttagtgctaa ctgttattgg aagtatatta tttcttactt tagcatcatt gggctatgta
    15241 gttttaaaac tcaaataaat ataaaaacaa ggctgatttt cagccttgtt ttcttttatt
    15301 tgaagattgg attcgcacta ataaatacat tatctacgcc aattctacca gcagagtcaa
    15361 ctacaaaatt atgacctaat ccgagaatac tttggttatt ataaagtaaa tttgcattat
    15421 gggcagggct atttttccat cctaccatgg catcgatagc accatcgtct gataaaaggt
    15481 catcaatata cagcccagtt tgacttatgt tttctccctt agcaccatag cagcctaagc
    15541 tttccaataa atcttggaaa gtaccaccac cagggcgata gtgactgaag ctgacttgta
    15601 attctttagc tcgaatatct gcagcctgtt gaacaatatc attgaattgc attggggcaa
    15661 caccattttg ttggcgataa tcatttaacc aagcaagttc tttttgacgc ataaagttac
    15721 gtaattcgtc taatgaaatc tttttatatt tgaatgtgat tgttttattt tgatcgacgt
    15781 tattaatgga tcgttggccg tttttatcca cggtaacggt aggatcgtca tttctgcaca
    15841 agaaataatt ttgatcagtc atatttttcc aaggagtaga gaagctatcc ccttcgtata
    15901 ctgtttttga ttcactagag agaacagcgt tggtatcagc atttacatga ttaacagtca
    15961 cattaaattt acgagtgtag ttgaaaatat aattttgact ttcagtcaca ttgtttaagg
    16021 ttactgaatt tgttggttct acccgatcgt ctaaaacata tccttgaata gaaggagcag
    16081 ttaaagtgac actttcaccg tgagtgacgt tgattatctg ttctttcaaa atatcaactc
    16141 gcccctcatt tttgtagttg ttattgcaca acaaattgat ttgatgcact tgattataat
    16201 tgaatgtgat tgttgtatta cctgtgattg ttttgctttg agtatcatta ccaatcactt
    16261 tgtagctaaa attatcttta gcaccttcgc tatatttaaa tgcttttgcc ttagcagtgt
    16321 aagtagaacc agaagtcact tttgcttgat ctgtagcaat cacttgacct gtttgattgt
    16381 caatatggtt aatggtaacc atatattctt ttggcgtttc aggtttgtgt tgcaaccctt
    16441 taaatgcagc atcaagacta tttttagcat tgtttacttg tgtttgagaa accttagaat
    16501 cgtctagtac ttttttagca ttggataaag cagtctgaaa attattccaa gattcttctg
    16561 taaaatctcc tttagcagtt gattttactc ggttgtataa agcttgtaat tcagctttat
    16621 tcacagtagg aactggtttt ttctttaatc ctttgtaagc agaatctaat ttatttttgg
    16681 cactatccac ttgcttttga gtcgcttttg agtcatttaa cactttctta gcattgttta
    16741 aagcggtttg gaatgcgttc caggtattgt cggtatagtc accttttttc gttcccttaa
    16801 cgctcgtata aagagcttct agagccgatt tattcacttt gggtttagag gtgtcagcgc
    16861 cataccaacc gataccttcg tatttccagc catagtcttt ggttaaccat tgtacttctt
    16921 tccaacttgc agtgtagtga tgtgagccag attttacacc aggaaggaaa aggcggtaga
    16981 tcggtttatt atttttttcg gcagaacgcc aggcaggccc ttcataagtc cagccgtaat
    17041 ttttagtcaa ccatttcact tcatcccaac tagctgtgta atggtgatca cgaagacctt
    17101 tattgtacaa acgatagaca agatggccac cgctagtcgg tgcttcccag gcggggcctt
    17161 cataatttcc ccattttatc tttactagat ggtcacgttc ttttttgcta gaggtgtaaa
    17221 aatgttcatt attatttgga ttgtaaaggc gatacatgac gttagttgaa gcagcgtcaa
    17281 cagatccagc aagactaagt gaggcagcaa atgttccaat agctaaaaca gtttttggat
    17341 taaataattt cattttttca actcctaaat ttaattaaaa atgtttacat gattattata
    17401 tatcggttaa ccgatatagt caataaaaaa taagaaaagc cttatataat tttggctttt
    17461 cttattttat ttgttagttg aagtattaat atcaacgatt tcttcagcca atttatttaa
    17521 tttcatagca tttatgaatc tcatgttgtc tatactagag cgtttgttgg caaggtcact
    17581 tactgtagtt aagggtacgc cagattctat ggatatagaa cgaactgatt taccactatc
    17641 aaacagcaac cattcgattt tctttttatc agcgtacatt tcaatgctcc ttcttgtttt
    17701 ttctttatta tagctctttt ttgtaatgaa tcacaatatc aagagaaaaa ttttctatag
    17761 aaaggtggat ttttatgagt attcgttctc cgtgtatcca aatgaggttt gttgctactt
    17821 tcgttatttg atatactaaa aataaacaac gaaagaagga ataaaatgaa gaaaaagcaa
    17881 ctatggtttg tctctatgtt tgcaatattt tttattattt taagtggatg ttcaagtaat
    17941 aagaaatcgg aagctaaaga agataatagt ttattgcaac caggaactgt ttggaaagaa
    18001 gaagtaggtg gtcttattca taatttaaaa attatagatg atactacctg ggaatattct
    18061 gaaagtgtat ggcatccaga tcctgtacaa ataacagtta agaggcaaaa agactatgaa
    18121 ggtttagaaa gatacaaaat agtagattct gcaggcatta gggaatttat aaacaaacct
    18181 gattcattat ttattgttgt tccctatgag aaggatggcg taaaaaaaat aatttttctt
    18241 ggatctagca aggatgagaa gcgaacgaaa gagaaattaa ttcatgatgg atcacaatca
    18301 aataaataca aattacaaaa gactagcgag tgattgctag tctttttatt tgtcctgaag
    18361 gaggaatcac agtggcaaaa gctaaaacgc catttgaaaa agccgcgtct aaaatcttag
    18421 aacgccgcgg tgtatctgaa acacagtgga aacaagaagt ccttgaaaaa gcacaatttg
    18481 acctattacg gggaaaagac aaggacttag aaggatatat ctatcaacgt gaacgtgaaa
    18541 agcttgtttt cgatgaaatt attaaacact aaaaggagcg aacaatttta tgtttgaatc
    18601 aattacaatg aattttttaa cacaaccgaa agtagtagct gcggttgata tttttagagc
    18661 attacaaact gccgcaacta gtttgaaaaa taatctgatg atttttgctg ctgttgcggc
    18721 agtggcttcc attgctcttg gaggtatttt cttcttcttt gggcgaagag gagctgaaaa
    18781 cgggaagaca cacgtaggtt atgtattgtt gggggtagga gttattttag ctgccgcttc
    18841 tattgtagtt tcaatgttct caatgtttgg acaaacccca caaggactat aaaaaaacaa
    18901 gtagaaggag taaaaagtac gtggctactt tttactcctt tttttatgga aggagaatat
    18961 tcatgttttt tacgatttca ggcaatgcaa aattggataa agagttaata agaaaagcaa
    19021 cgaagaaagt gggaaagcgt acttcctttt taccgaaaac aattaaatat tctgtcattg
    19081 aaacggaaga acagctacat gtgttaggac aagtgttaga acaagagaat caatcttctg
    19141 ttaaattgac tctcacgtta catgataaag aaggaaaaat agaaaaggct ggcacagaat
    19201 acgtttttgg ctcactggat tatcaaattt cagaaggttt ttatcctaca ttgattgatt
    19261 taatccgcct agatatattt aaagatacgg atgaaaccat gagtgtggaa gagtcttatc
    19321 gaaacgaaaa aattttacaa gttttagaag aagagttaga aagcgaccaa agaaaagtga
    19381 atcaagtgag tgaagtcgaa gcaaacgaag aagtaccatg ggaacgccca atctttccaa
    19441 aggatgaatc aaaagaaaaa gagaaaggaa ttattgacga acaagctgac attttgactc
    19501 ataaatcaat atttgaggaa gagaacgagg taatgggtca attggaggta gatccctttg
    19561 aagaaacaat ggaggatgaa gagacggccg aagtagaagt tgatccagtt gaagctaatg
    19621 aagacccaac atggactgaa ggggattctc aggaatatat tgaaccactt atcagtgaag
    19681 aagtagagaa aaatgaaaca gagatacaaa aggcaacggt atttgattat attttggatc
    19741 aatatccgcc agagcatgaa tggatgaaag aaaaaaataa attattttta aatgaacttt
    19801 attcgaaata tcaattacct caaacctatc aagcattttt agagagtaaa gaagaattaa
    19861 tctcacaggg taaaggcaca cttttagaac agttagagtc tgtccaatca acaaattggc
    19921 atgagcttgc acaagaagat ttattagcta cttttgaaga acgtcaacaa gaaacagatc
    19981 aagaaattaa tgagtatgcg acggaacagt cgtcgaattg ggagaaagca aaagaggaac
    20041 taaataaaga agaacagcga gtgattgaag aagaagtaca aaaaattaaa gagcggtacg
    20101 aaaagaaacg tgacaatgcg tattccttgt gtgtagaaaa gatcaacaat tttactcaga
    20161 cgaatcaaga agcattatcg aaagaaaaag aaaacttatt aaaagaacgt ataaaagaac
    20221 gaaaagaaga attttaccga gggttgcgta ctgataagct aaggattagc aaagaattaa
    20281 atgaacaact gaataaactc tatcaaacta cgactgaagt tctgatggaa aaacacgagc
    20341 agatccaaca agaattacaa gcacaaattc caaaatggaa aaaagagtat gaaattcacc
    20401 aacaagaatt gaaagaacaa gaagaaaaac aacggaaaaa agaaaaagag cgcgaacaat
    20461 tagaactaaa aaaacgtgaa caggatcttg cggaacaaaa aatggcgata gaaaaacaac
    20521 ggctagaaca agaaaaggaa aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg
    20581 atacattgcg acaagcacag ttaaattatc tgacacatcc tcaaccagta gctgctgttc
    20641 aaacgccagt gaacaaccaa gcaaatttaa ctccttcacc ccagcgttcc tttaacggat
    20701 ggatggtggg atgtattgca agtttaagct tgcttctagg tggaggaaca gtatttgctt
    20761 ttaatcatat caatcaatct tcacaggctg attctgtagc cacaacggcg tcaatcaagg
    20821 aagaagccca ggaacaagca agaaaagagt atgaacaaca attagaagaa ttattgaatc
    20881 gtaccaatca gtcaacaaca gagtcaagca tggaaaatag caaagagagt gattctagta
    20941 aggctaaaga aggatcaagc gattcaactg tacaatcaaa cacttccgaa agttcagaat
    21001 caaatgaggt gaaataatgc gtgttagaga aacaacgaag cgaaaagaca aacgattcaa
    21061 tttagaaaag aataggtatc tttctcattt ctatcgctat actccctcag gagaagagct
    21121 accagaaaaa catgacaaac gcatttcgaa aaagattttt tatagtggac ttgtatacct
    21181 ccctttagag tatttagtaa caagagtacc agaagttttc aaccgattgg aaattgttaa
    21241 gggaagctta caaggaataa atctgtttca attacaggaa atgccacagg tctttcattt
    21301 tgattcagct ggtttttgga atccatttac tcctacaact agtttgggta cacgtttagc
    21361 aatttcgaca gtttgtgtgg ttccagctgt acttatggga ttgaaaatgg acatgtggtt
    21421 tcgtcctcta gcagatggac aaaaagggga taatcgatta atgaccttaa aagaaatcca
    21481 acagatttat ccgcaaatac ctgaaacaaa aactactttt ccaggttatg gaggtattcc
    21541 aatcacacat tacaaaaaat attggtacat tcaaacagat actgttaata cgtgtattgt
    21601 tggtacctct cgatcaggga aaggtcagat tgaagtgtta gccaccattg acaatttatc
    21661 tcgtgcagaa aaacaaagtt caatggtcgt taatgatcca aaaacagaat tatatgttgg
    21721 tagtaaagat gaactagaag ctagagggta tgatgtatat gcctttaata tcaccgatcc
    21781 actgcaatca atgagtgata atccattggc actcatcgtg aaatactgga aacgaggaga
    21841 catagatact gcaacacagt taaccaatac tttcacaaat acgatttatt atgatgccaa
    21901 cgcttcagat aacaaatact ttaacgaaaa cgcgcagaaa gctgtcaatg cgcttatttt
    21961 tgcgttgtta gaacaagctg atcaaacagg agattactcc aaattgacac cgaataatct
    22021 cgttgagctt ctttctgaga ttggcggttt taattattat ccagatccag acaatgaatt
    22081 caaacagttg aatgccttag atgaatttat gaatcagttg ccacctggaa acattgcaaa
    22141 aaaacaatat ggggcaacga aaattggatc tgataaagcg aagggaaata ttttatcaac
    22201 ggcaatcacg ggattgaatc catttactct aacgaaaatt gcaaagatga cttctcaatc
    22261 aacgattgat tataaatcgg ttgggtttcc aaaatactta gatttgaaat tacatgaaag
    22321 tcttttaaat caacgagtcg tagtagaatt ttatcataag ggaaagaaat ttcatgaaga
    22381 acgtgtcaaa gtaggctata aaggatttag tgaaatcaat tttgattgtc atttagttgc
    22441 aggtgatcaa gtttgtttaa aatacgatca tcaaggaaaa gaatatgtgg cttggtatga
    22501 attttctcaa agagtattga aagacgaaga gggtaatatc gtttatcgga aaaaacgagg
    22561 agaaacccat ttgccagaat tagaaaaaga agccgttcta acattagatg aggaaaaaag
    22621 taacttatta gtagagtcta ttcggatgca ctataccgat aaaccaatgg ctatttttat
    22681 gcttacacct gattatgatc catctaacca tatcattgtt agtatttttc ttagtcagtt
    22741 atataaagaa ttatctacgc aatgtgttaa gactaaagga gataaatgtc accgcaggat
    22801 tcattttatt ttagatgaat ttgggaatag tgtgacctgt ttagcctaag gataaaagag
    22861 gctagttcca acgaacattg gaataacttt actatcgaga aatcaaatga cggggtaacg
    22921 ccctgaaggg tttccactga tactgcgaca tgcgttagta ggatgtgcat ccaaacgcgt
    22981 atgaagctcg ctgaagtcgg cagaagggta ccgtaaggaa agacacgata cgtatctacc
    23041 acgcaagtgc tctagaggta gggtatgtac ctgatatgtc gggtgtccta aaaagttcaa
    23101 taaggtgaga atgatttgat tatctgacaa acttgcgaat ggaatgtctg aaacgaataa
    23161 ctcatagaaa tgtgaggggg aagggggata cgtcctcttt ccattgactc gaggttgagt
    23221 aaaaatgtgt ttctatgaaa aaccttgcat ggttaaaggc tatggagtag tcaacaggca
    23281 aaagcaagca cctaagttga aatctatgga tagatagtaa ggaacagggg aagcatctag
    23341 cacggaggga gcataccgaa gatgtgtttc taaggaaaaa ccgaaatcgg ttataactta
    23401 ggtgaataga tgtgaaagta gagccacgac cagtgaaaat ttcgtaatga aattggagga
    23461 acaggctcaa gtcgttattt ataatataag aaacctattt aaaatcatgg attctcgtaa
    23521 gactaaaaag gtcacgaacc agaatggagc gtgataaaac catgactaac acaaaaggaa
    23581 ttagcttaaa gaacaaacaa ctcagacacg ccgaatacta cgacatgact gagactttcg
    23641 atgaattgca cgcaatgagt gttgcaaaat caaattttaa aaatctcatg aaattcatta
    23701 ttgatgacaa taatatcttt ctagcgtata gaaatatcaa aaagaataaa ggaagtgcaa
    23761 cggccggata cgatggtgtc actataaaag acattgaaaa attggcatgt ggtgactttt
    23821 taactattat taaaaggcga tttaaggatt acagaccaag aaaagttaaa agagtagaga
    23881 tcccgaaacc aaacggaaaa cttcgacctt taggaattcc ttcaatatgg gacaggatag
    23941 tccagcaatg tattctacaa attttagaac caatatgcga agcaaaattt aataagcata
    24001 gctatggatt cagaccaaat tgttctacag aaaacgccat agcagattgt cttgctagaa
    24061 tcaatcaaag taagatgaat tatgttgtag atattgatat aaaaggattc tttgacgaag
    24121 taaaccatac caaacttatg cgtcaaatat ggacaatggg gattcgagat aaacaactcc
    24181 ttgtaattat tcgtaagatg ttaaaggctc cgattgttat gcgaaatggc aatgttgttt
    24241 ttccaaccaa gggtactcca caaggaggga ttttaagtcc actgcttgcg aatatatgcc
    24301 tgaatgaatt tgattggtgg atagctaatc aatgggagga acgaactgtc aaagagctga
    24361 aacaccacta tcgaaaaaac ggagcatggg ataaaggaaa tatctattcg aaattaagaa
    24421 gccacacaag tttaaaagag atgtatctgg tacgctatgc agatgatttt aaaattttct
    24481 gccgaacagg agaagaagcc caaagggctt tttatgccac acaaaaatgg ctcagagaaa
    24541 gattaaagct cccaatttca atggagaaat ctaaagttac gaacctaaat aaagctgaaa
    24601 gtgaattctt gggtttttca ataagactcc acaaaaaggg aaaaaagaaa gtggctttca
    24661 cgtacgtttg tcgcaaagca gaagaaaaca ttaaaaataa actaaaacaa cagattaaaa
    24721 aaattcaata cgctggagca aaaaaaaata ggcaccgagc aataggtgac tacaacatga
    24781 tggtcatcgg tatccacaat tactatcgga tagcttcaca ttgtagtgtc aatttcactg
    24841 agatttatag aagtgttagg acagtaatag aagcaagact gaaagctaga ggaatgacga
    24901 aaaatggaat ctatggacaa gatcatgaag gataccgtcc gtatctaaag tctaagcaaa
    24961 tgagatatat tgaaggtttt ccgatacttc caattggcta tattaaaaaa agaactccac
    25021 tcaacaagcg gagaaatcta aataaatata ccaaggaagg gagaaagctc attcatcaag
    25081 agcaacaagc tatagaaagc tggaaaatca agtatcttgt aaaaggtttt agtaacaata
    25141 agaaatacac ggtggaattt tctgataaca aaatatctaa gtttatttcc caaaaaggga
    25201 aatgcgctat cacaggaaac gaacttttga tggaagaaat tgaatgccat cacattaacc
    25261 catatcattt gtccaaagac gatagttaca agaaccttgt cattatctct agagaaatcc
    25321 atagattaat ccatgcaact agtgaagaaa ctattctgaa actgcttcgg ttactgaaac
    25381 taaatcacta tcaaattaga aaactaaaca cgtatcgaaa aaaagcagct aattttccaa
    25441 tatcaatcaa aatgaattag ataggaaaca aattatattg taattaacga tggaacgccg
    25501 tgtgcggtga aagtcgcacg cacggtgtgg accgggggaa aagacggaga taatatcaaa
    25561 ctcttaccta tcggaattgc caccaatgga taatatggaa ggaatcatga cggtcacagc
    25621 tggccgaaac atgttgtggg atctgtttat tcaatcttat cagcaacttt atgcgaagta
    25681 tggagaacaa aacgcagcga ttatcaaaga aaattgtcag acacatattt atattatgtc
    25741 gacagatgat aataccatcg aagaattttc taaaaaagtg gggaataaga cggttgaaca
    25801 agagaacagt accatcaatt cattaggtat gaatactcat atcaatcgca atgttgattc
    25861 agatcgtatc ttaactcctg aaagaatttc tactttttta gaaggtgaaa cgattgtact
    25921 cgctccttta aaacgacaag atttgaaagg tcataaaata cggccgtatc ctatttttaa
    25981 tcatggggaa acgaatcttc cttatgctta tcaatatatt gaagatttca atccatcgtg
    26041 ggatatcaat gatatagaca tacattgtac acatgcggac ttggatctta aggctttaca
    26101 aattgattat gattatttcc taccagatga atatgctaga aatctctatc atggactgca
    26161 tgatcaatta gaagaaaaaa acgaagaaaa tatgttttat aaaatcgttc aatctcttga
    26221 gaatgagacg gctaaaaaat tgttaattca attttacgaa gaacaagcag cagatcgact
    26281 tcaagaatat gttgaaaagt tgatcaaaaa tgaagaaatt tctgaaatga ttgggaaacg
    26341 gttgttattg gaatctgaaa aggtggtgaa agaatgatca ttggaggaat tttttctggc
    26401 atagccgact ggtggtcagg cttattcgga gaaggggatg caaaccccaa aaccgtaacg
    26461 aaagtcttac aagattatgg taattatctg cagtatcaag atgtgttcag ttggatttgt
    26521 aatacttttt tatgggggtt gatcaaaggg ttatataatc tcaatacttt actagaaaaa
    26581 accatttatc aaagctttga tttaaaagac ttattgaacg ccgctggggt aaatgaacta
    26641 tttcaaagtt tgattagtaa ggtattggct cttttttgtg tcgtcacatt aatttattta
    26701 ggcttgaaat tttttatgag taaacaccca ccaaaagtca aaaatatctt ggttaattta
    26761 tttatggcca tgatcttgat ttttggtggt agttctttaa tcgatgaagg gttaaatatc
    26821 agtaaaagct tttacggaga cgtcactgca gcaaataaag cagataaaac gggttcacct
    26881 gcttttcaaa ttattcaaaa taatgtgatc gatgtcagta cattactagc aactgacccg
    26941 aataaagtag aatccctgcc aacagacaaa agaaatgctt taacagcgaa aaattttaaa
    27001 acagcgaata tcaatgggat tattactcct aaacaagcta aagaaatggc ggataaagat
    27061 aagagtggtt tatcagattt acaaaaggat cggttaagag cgttacaata ccgactggaa
    27121 ctggatgata atggaaaaga aattcctgtg caaatcagtg atgaaggctt agccaaatat
    27181 gtttatactt cagggtatcg tcgatacatt agtagtccag ggattatttt agctggtgaa
    27241 tcttctttgg cggtggccta tttatttatc ctatttacga tcgttacttg cattattgag
    27301 ctggtattta aaaaattcta tttagtgatt gccgcgtcaa cggactttga gacaggacaa
    27361 cggatgaaaa gtgctatcga agatatttct caatccttct tgttattggc atttacaatt
    27421 ttagaattac gaatctatac cttaatgctt tcaggtattg gtgatttgca tgcggcagga
    27481 aagatcaatg gattcttgta tgttgtcgct ttgattgtac taaccattgc gctattcaaa
    27541 ggctctcaat cagttacgaa aattttcggc gttgatacga gtctgaaaaa cggcagtaat
    27601 tctcttctta gcatgtttgc cttaggaaat attgcgaaaa atgtgggtag cggagctaaa
    27661 aaccttgcaa gtgctggtaa gagtggttta agccatttaa atgatattcg taaaaatgga
    27721 tttggccatc gaaaagatgt tacccctgat aacggggaag aacaatcaga cgataaagct
    27781 acaactgtac ctaatagtaa gaaaacagtt tccgaaatgt tcgggaaagc gaaaaatggt
    27841 gtttctaaag ccgcagaagg tcttggttac gtcaatgaac gtgggttaaa aggaacagct
    27901 gaagatctta cagaaaaagc caaagacacc ttagaagata aattagacgg cacgagagct
    27961 aaagaattgg ctgaagctgt gaagaatcca tccgaagcac tagaaaaaca aaaagaaaaa
    28021 gcttcagaag tcaaagaagg actaaaagat gcgtatggtg aaggacaagt caatgcagca
    28081 gtgaaagcta atacaacaac cgaaaaagaa aatcaagaag gtatagtgat tccttcagtc
    28141 aaagatcacc aagaagcgtt gcactctaat gaaaattcga ttgaagagaa aaattccata
    28201 gaagaaagaa attcagaaga aggaaaaaat ggacagttac caaaaactga tccagaacaa
    28261 tatcgctata aacctaacag tgaccttgaa aatgagaagc gaatttcaga tttatcaaaa
    28321 gtaaatcaag aagacaaagc agaaaatgag ggacagttac caaaaactga tccagaacaa
    28381 tatcggtata gatctgttaa ctccgaaaat gaaaaacgca tttcagattt accaaaacga
    28441 aatcaagaag tacaaacgga tgaggattcc atcgttctgc caaatgataa tggaatcgaa
    28501 gttggaactg aaaaatcaac gaaaattggt ttacctaagg cacaaaattt accaatgaaa
    28561 gatagtcgtt tgaatcaatt caaggataat gatcaaagtt ttgttcatca atcacctgca
    28621 ggtactgttc aaatgaaacc aagtcgtttg cctgaaaatg gaaatctgac aactgtttct
    28681 tacaatttat ctgaaagtgg tgagacaggg aatgcgtccg ttcaaaatgt tgtgacaaga
    28741 gaagctgcac caaaacctat tcaaaacgtt catacaggtt acaagccagc aaatgtggca
    28801 tttactgcac caacaaaaat aaatgtgaca agagaaacta aaaacaggaa caatgcattg
    28861 aaagaacaac tattgaggga tatcaaacag gatatcaaac cataaaaggc ctacaagtta
    28921 ggccttttct ttatatgaaa aggagaaatc aattatggat aaatatataa acatgaaaaa
    28981 ccctccaaga tttagtggag aatggaagtt tttcgctttt ttctttttag atctattaac
    29041 gcttggtggc tgcttttttt gggcgaaaat ggtcaataat cttgtccact taccactctt
    29101 ttttgcactc ttgaattatg gcttatggat tgcgatagcg ttagtgattg tttggcgacc
    29161 ctataataac ccaggtcaac gacaaatttt tgcgattatc gagtctattt tttttgcgga
    29221 caagaaccat tatcatgcgt tagatcctaa ccgaaattat catggtacgt ataaggagga
    29281 tgaataaatg cgagtcaaag gacaaaattc agttgaaagg aaatccattt tgcagtttgg
    29341 aaaaccagaa gagcgctcta tcttggactt atttccgtat aaatcctgta cagaagaggg
    29401 agtcttaatt actagagaag atcattttca acgttattat cgtgtagttt ctacagacgt
    29461 ggaaggatta aatgaacaag agaaactaga acgaatgaat cagttgacca ccgtcatgcg
    29521 tacgtatgtg ccaacaatta agttagttag tttgaccaca gagacggatc taacggaaca
    29581 aatcgtgcaa aagcggcagt tattagataa aaatcgtatg gaacagtcct taggaagaaa
    29641 tctacagcga ttaagaaaat atgaaaagaa attagtagag gaaatccagg aattgaaacg
    29701 agcagaacag gaacgccctg atttaagctt ctttttcgtc attgaaggaa agacaatcaa
    29761 ggatttgcaa accagaaacc gtcagttaat gcgatctagt ggcattttag ggttaaaacc
    29821 actctacaaa aaagaattga tcaagattct ttatcgaatg aataatatga atgatgagta
    29881 ggaatgggga agagagaatg aaatatccgt tgatatggtt agaaaaagat gttccgaaag
    29941 aagagaccac tagaaaattt cctcacactt taagcctggg taagttggga aaaggaaaat
    30001 cttttaacca agtaaagaat caaacaagaa gaggtgactg tacatgttag aagcaattaa
    30061 aacagctttt tcaccacaaa atgatcaaaa ggtacttgat ccattaaagc aactaggaaa
    30121 tcgggaaata gaaattttaa aatccaaagg gtatgattta gactttttaa aacaagtgat
    30181 gccacaaggt ggattaaatt tcgatgaaga gtatgtgacc tatggaaatg gttattcacg
    30241 atgtgtgaca gtttatcact acacaaaaaa tcctacgctt ttttggttgg ccaatctcat
    30301 gaacaatcct catacagtgg caacactaga tgttagcaca gatgaaaagc aaaaagtgat
    30361 tgatcaaatc aatcgtgcct tagatgaatt aaatgatcgg tcagataatg aacgaaaagc
    30421 gactgaccga aataaaagcg cggaagaata cctagaacta gcggaatatg cccgaaaatt
    30481 gacttctaat ggggaaattt ctaaacaagt caaagtgaga gtacatgttt acggaaacac
    30541 gattcaagaa gtagatgaac gagcacagga actgatcgaa aatctcaaag ggatggatta
    30601 tcgtgcagtg acctatctgc acaaaacgaa aagagactgg caatcgatgt tcaccatgtt
    30661 cgatgaacaa gctgaatggt ttggaaatat caaagggcaa tcactaccat cgctaaacat
    30721 tggcggaggt ttcccgttcc accaccaaac gctaaaagat ccaggtggaa cacaatatgg
    30781 ggaaaccatg acaggcggcc cgtttgtttg gaatgccacg tataaaaata gtgtgcggtt
    30841 gtctaccaat accttgcttc taggaatgat gggagctggt aaatcgaccg ttcttaaaat
    30901 gatcgcggaa gcccatttag ctgcaggaga ctttatttgg ggctttgaaa agggaaaaga
    30961 ctttattccc tttttaaagg aatataacgg cattatggtg cgtttagacg gatctgacgg
    31021 gatgatcaat cctttagaaa tatttgccac acgtacgtat gatgaagctt ccagtttata
    31081 tgacgatgga tcagtcaaag atttgaagat caatgaagca gcgtcctatc aaacgcactt
    31141 agacaaagtg gtctatcagg tacagttagt gagtcctcag ctaaaaggaa cgatgaaagc
    31201 agaatttaag acctatttaa atcgttttta tgaagagtat ggaacagtac cacgcggctt
    31261 tacttcctca aattcgcgtt caaatactga tacgcaagtc acaggtaaag atccagaagc
    31321 ttatccgacg ttcaaagaat ttttagactt tttaggacaa ctggaattac caggtgcttc
    31381 ccaagagaaa aagaatcgaa aagaagaaat ggaatccatc gtagaatcct tatgtgagac
    31441 gtatggcatg atttttgatg gtcatagtac gattcgacac cttgaccaac aacagttggt
    31501 cttttttgac attgactcta ttacaggctt agatcgaggg gtacaacaat gtcaaatgta
    31561 catggctatg acgttgattt ggaatcaggc attgattcaa ggaagaagac aacgacgttt
    31621 aattaaagaa aaacaaatgg aagaacaaga cttcaaatgc ttcttggcga tgattgatga
    31681 gtgccataat ggtgcgacct gttctgcagt gaaaagctgt gggttgcaag tagtctaaaa
    31741 actattgtaa taactcttat aacgagaagt caaatgagtg ggtaacgcca caaagggctt
    31801 ccactgatac tccgacatgc aacaagtagt ttatgcagac caacgttgtg tgaagctcgg
    31861 tgaagtcggc tgaaggatac cgtaaggtaa aagaaatagt gaataccacg aaagttctct
    31921 agaggtaaag ggtgtatccg ataagtcggg tgtccaaaaa aacttaatat ggtgagtatg
    31981 tacaaacgta ctgacgaact agcgaatgta ctgtccgaaa ggcatagctc ctagaaatgg
    32041 gagagacagt gagcaaaaaa tcactcattg tgctaacttg tggttgagta aaagtcgttg
    32101 ttatgaaaga ccatgctgcg ttacaggcgt aggagaagtt agggggcaaa agctagcatc
    32161 taagttaagg catgtggata gatataaggg aacaggggaa gccatcaaca tggagagtgc
    32221 atactcgatg aagtggtagt aagaaatatg tgaatgacaa ataacttact ttagtgatgg
    32281 tgaaagtaga gtcacgactg atgaaggttc tgtaatggaa ctggaggaac aggctcgatt
    32341 cgtctgtaaa agtcatgaaa cccgttcaac catgggattc tcgtatgact aaaaaggtca
    32401 cgagccaatt ggagcgtgat aaaccatgac aaaagaaaca agtccaaaag ataggaagtt
    32461 gcggcatgca gaatattatg gcatgacgga aatcttcgat aagatatact ccaaaagttt
    32521 gaagggagat aatttcaaga atctaatgca gattatcatt tctgataaca acattgtcct
    32581 agcatatcgt aatatcaaga gaaatcacgg tagtgtgaca agaggagaag atggagtaac
    32641 gattaaagat attgaaaatc tcaaccgtaa tgaattcgta gaaattgtta ggaagcgatt
    32701 cgcacattat aaccctcgga aagtaaggcg tgtagaaatt cctaaatata atggtaaatt
    32761 aagaccgttg ggtattccat caatatggga tagaatcgcg cagcaatgca tcttacaagt
    32821 attagaacca atatgtgaag caaaattcaa taatcacagc tatggtttcc gcccaaatag
    32881 atcagcagaa cacgcaatag cagattgtat ggcccgaatg aatcaaagtc acatggaata
    32941 tgtggtagat gtagatatca aaggtttctt cgacgaagtc aatcatacta aactcatgag
    33001 acaaatctgg tctatgggta tacgagacaa acagcttctc ttaatcatta ggaaaatgtt
    33061 aagagcaccc gtagtacttc caaatgggaa gttgatatac ccaacaaaag gaacaccaca
    33121 aggcggaata cttagcccat tacttgcgaa catcaatctc aacgaatttg actggtggat
    33181 agcaaatcag tgggaggaaa aagatgccaa agagctaaaa catcatttta ggaaaaatgg
    33241 agcttgggat aaaggtaata tctatcacaa gctcagaaag agtacaactc taaaagaaat
    33301 atacctagtg agatacgcag atgattttaa aattttctgt agaaattata atgaagcgaa
    33361 aaaagttttc tatgcaacta aaatgtggtt acaggagcgt ctaaagttac caatatctga
    33421 agaaaagtca aaaatcacaa atctttcaaa agaagaaagt gaatttttag gctttacgat
    33481 gaaattagtg accaaaggta acaaaaaggt tgttaatacg catataggta caaaagcaca
    33541 aagcatgatt aaagtgaaac taaaaaaaca aatcaaacga attcaaaaaa gtcctaatag
    33601 caaagatacc tttatctata ttggagtcta taacagtatg gttattggga ttcataatta
    33661 cttcaaaatt gcgactcatt gtagtgtagc aatgcgaaaa attgcaagag aactcagacc
    33721 aagttttgaa atacgactac gagaacaagg aatctcaaaa gaaggacaat acggaaagta
    33781 cgaaggatac aagccttacc tacggtcagg acaaatacga tatataagag ggtttccaat
    33841 attaccagta gggtatattc aacacagaca tccgatgtgt aaaaaaagaa atattaataa
    33901 atacaccgaa gaaggtaggg aagccattca taagaagcaa caaaccgtag aagaatggaa
    33961 gatatcgtgg ttgtgtagca atccagtgct tagtaagaga gcaacgattg aatttgcgga
    34021 caatcgaatt tcaaaattca tcgctcaaaa gggaagatgt gcagtaacag gtgaggagct
    34081 tatcttatct gaaatgcatt gtcatcatat tatcccttat catgaaagca aaagtgatag
    34141 ctatgaaaac ctagtgattg ttactgagga ggttcatcgg gtaatacacg ctacacaaag
    34201 tgaaacgatt gaggaacttc taaaatatct aaaattaaat ccaaaacaaa aagaaaaact
    34261 caatgagttg agattaaaag ttggtaacga agaaatatct taataaatat ttgtaggacg
    34321 ggaaaacaga atgtctttta tagacgaggg aacgccgtat gcggtgaaag tcgcacgtac
    34381 ggtgtggact gggggaaaag acggagataa tatcaaactc ttacctatca gtatcgatta
    34441 atccagattt tattgaaacg gtaaattatg tcacaaactt tcaacgagaa atgcgtaaag
    34501 tagaagcaat gactgtttta gctacacaat ccccacaaga aatggtgcca gaaaatatgt
    34561 cttctgataa cttctcgaaa ctgaagaaag tctttgaatt ttcttctacg aagatcttta
    34621 tgcgtatgga tgaatctatt ttacgtcata tagaaggatt agttggtaaa tcaatgacaa
    34681 actcagaatt agaatccatt cctcaacaaa atcaaggaga cgctgtgatc aattttggct
    34741 caaaagaaac cattcgtgtt cattttagtc cgaacaaacg tcaattaaaa ttgtttgatg
    34801 gaggaaaata atggaaaacc aaaatgaaag tttaatcaag cagtatgtta agcgtcgtgc
    34861 aaagcgacgc ttatttttgt ggttgtttgg tacttctgca ggtttgatta ctattttgat
    34921 tactgtgttc gtgacccttt ttctaatact tgcggcgggt tcgattgata attctgattc
    34981 agattcaagt agtggtggcg aagcttttac aggagaatat tcggaaggct taccgattta
    35041 taaagaaatc aaaggaagag gccctttttc tgatgagatt gcgcaatatg ctgtaggtgc
    35101 agcagtgaaa tataaattat taccaagtgt gatcctttcg caatacggtt atgaatcagc
    35161 gtttggtacg tcggcgtccg caagaaatga tctcaactat tttgggatta cttggtttga
    35221 tggttgtctg tttcctaaag gaactgcgcg aggaatcggt ggtattgaag gtggctggta
    35281 catgaagttt cccaactcaa aagcagcctt ctcctattac ggctttatgg tagccacaca
    35341 atccaatttt aatgcatgtg taggaaataa aagccctggc gcaagcttat tgatcttagg
    35401 acgaggtggt tatgccgctg caggaattac tgaagatagt ccgtattata caggttgtat
    35461 gtccattatc acttctaata aattaaccga atatgatgaa ttcgccatca aacattgggg
    35521 tgaaggtggc aataacaacg gcacgatcac tggtgaatgg acgaatccat tccctggcag
    35581 cagtttagat aaaagttcct tcagtggtgg tcaattattt ggaacaaatc caggcggtga
    35641 attccgtccg aatggcttcc atgatggatt agactttggt tcagtcgatc atccaggaag
    35701 tgaaattcac gcggtgcatg gaggaaaagt cgtgtacgtt ggcaatcctg gtatttcagg
    35761 tttaggtgca tgtgtgatcg tgattaatta cgacggtttg aatatggtat atcaagaatt
    35821 tgcgaatagt acaggaaatt ctcgtgtgaa ggttggcgat caagtgaaag ttggtcaagt
    35881 aatcgggata cgagatacag cacacttgca tttaggtttc actcgaatgg attggcgtca
    35941 agcacaagga cacgcattca cagacgatgg tacatggatt gatccattac catctttgaa
    36001 tagcagcaaa aaatagatca ggagaaaaaa tatggaaaat aaaactttaa aaatcgtctt
    36061 aacagctgca attagcgtta gtttaatcgg caatatcgcg ttgttttggc agctgaaaga
    36121 agaacaagag acaaatacct ttaaaattga agaagtggct aaaaacgcgt ctgagaagct
    36181 caaaaaaaat tcctctgagg cacaggcgaa gatcgacaag ttaaatcaaa ccatcaatga
    36241 gaaaaaccaa gaaatcgatc agctgaagca acaagatggc acaaaagcaa aagaggaagt
    36301 aactgaagca caaagaaaga cggcagaaga gttcggtcaa ttggccattg ataaatcatt
    36361 aagtagaaat gaattaacgg aaaaactaaa agatgtcgca acccaagaag tgatcgacaa
    36421 attaagtcct gcagacgatg atcaccaaca tgatgattac ggaagttact cctttacatt
    36481 aggtgacgtg caaacttatg ctcaaacaag cagtgtaaaa gaggaacaaa attttgtcgt
    36541 atttgttgat tatacaatcg gcaatccgca attcaaagat gtcaaaccac aaaaaataaa
    36601 aggtggatta actgtcactg aaaaacaagt agatggtcaa tggaaagtca tagactttag
    36661 ttatttcact cgttaggagg aagaaaatga gtaagaaaaa aggagaaaga caacgtttca
    36721 aaaaacgaaa aagttatctt caatggctgg gaatctttat gattctcggc tttttgattc
    36781 ttgctagtcc gatttatacg aatcagaagg tagatatacc agatgaggta agcacgaacg
    36841 cctttttaaa cacgaaagag ctgacaatgg tttcaaaaga aaaaggagcg aaccagcaat
    36901 tagtttttga atttgcgatc gatgatgaag atcaaagctt gttaaaagaa ctggctaatc
    36961 ttaattatcg cgtagaagta aagacagcta aaggggatta tcaagcaata cgagccaaag
    37021 tgatcaaggt gtctgatgat tattttgcag tgaagctaac gcatgtacca gaagaatata
    37081 tcgccatgcg tttaaccatt attcctgaaa aaattgatcc aaaagtggat atgcaagaac
    37141 cacaagatct gatttattac gtgcacgaag ataaggttaa agatcaagtg aaagatgaag
    37201 attatgaaca acacgcgatc aattacaaag tgaaaggata caagaaagaa caaaaagcag
    37261 ctcaaaaaca aatggaatct ttccaagcaa ccattgattt gaatgaagaa ttagtgaaga
    37321 agctaaaaga tcaattgcct tatcaagtcg cagatgatca ggaaaatact gaaaacaaga
    37381 ttaatagcta ccagcaagaa attgaaacat cgaaaacgca actgaaagat caagaagaga
    37441 tcattcaaaa acttcaagaa aaaattgatc gtatgacaaa agaaaagatt tagaaggaca
    37501 ggtggagtga aaaaatgaag cgaacaaact tggctgacaa agatactttc gctgagcaga
    37561 aagtattgat taaagcctat ctcaaagttg taaaagctaa agaaatatgt cgagaagtga
    37621 atttatctaa cacaacaata tctgagttga agaatggtcg aaagaattta gatactttaa
    37681 aattcagatc atttgaagct ctatatgtag ctgctaaaaa atattttaca gctgataaca
    37741 aataaaaaac aggagtattt tatgtgaaaa aagtatataa aaaatgctta tgaatcagct
    37801 atatcacctt aaccatttgt taatgagatg ggagaaaaca ttaaccacga ttctaacgca
    37861 aacatcaagc acataaaaca tttaacactc ctcctttctg gctgaagatt tcaaccaaac
    37921 aggaagacag cgtatcaatt atagcataca ctttttctta aattcatttt tcatatgaca
    37981 aaatatttcc ttctaatcga caaagttaga aggatttttt attgtctaaa tacacaaata
    38041 accttaattt ttaaagagca taaggaaacg tgatatcact ttctcacgaa aggtgttagg
    38101 taggctgtgc ctaccacgtt tctaagaaag ataaatgata tcaaaatttt ggatttgtgt
    38161 gtagatttgt gggtaggatg aaagcgaatg tgggcagtaa aataagcgtt tgtgtgtagc
    38221 aaaatcaaaa aatgtgtagg gatatgtgta gctacacata tccctacaca taataaattt
    38281 ggaggaaaat atggacttat taattagaaa tattgaagaa aagtatatta ataaaattga
    38341 caaacgttgt gaagaactta cggtcaagac tggcaaaaaa tggagtcgaa atcaatacct
    38401 aaaagttttg atcgaaaatg attttgatca cgctcttctg aattataaaa aagaccaatt
    38461 tgatcggttg ttagaaaaat ttgtggatat tcaaacttat aatacgaaaa ctttagaaga
    38521 atatattgca acaaataatc aattaattgg tttgttaatt gaataataaa aatagtattg
    38581 tgaatttata aaaggaggaa atcaattttg gcaaaagcaa caagaaaaca tgtatatctt
    38641 tacgatatgc atatcgaagt acttgatgaa ataatttcta ctcatgaaga tatatctaat
    38701 tatagtgaag cagtcagaca tttgtgtatg atgtatgatc caaaaatcga agaaaaaata
    38761 aatccaattc aaattaaatt gaatgcaatg ggaaaagaaa tttctatttt aaccgaaatg
    38821 gtttcagagt ttgcggatct tcatttgaaa gatacaggaa tactaattgg tacaaattca
    38881 actgtttatc aagaagcaga agaacgtgtg gaaggaaaaa ttaaatccaa tcaaaccaaa
    38941 aaatattctt ctaagttaaa gaaaccagtg aatgaatctg ttcataccag agtagatcca
    39001 tttgatccaa cgaagattct aaaaagataa aagtagaagg agaacgtgat gaataaatcg
    39061 cctgcagtta ttcttacttg tcaatttacc ttgcccaata aaaaatcttt ttcaacatac
    39121 attgattata tgacaagaga aaaagcactg gaagaaatgg aacgacgtac gcccgaagaa
    39181 gaacaggaac tgcaacgaat caagaatgct ttagaggatt tttatattcc tgaaggagaa
    39241 actttttcgg aaagtaagac ggataaagaa ttcagtgaaa cgtcgaatga agctcaatcg
    39301 ttgatggaac atggaatcaa ttttgacaag ttagaggaac aagattttac caaatatctt
    39361 tcttatatga cccgtcacta tgcgttagaa caaaagaaag aactgacgca aagggaacaa
    39421 aaagaatacc aacggttgaa ccaagcaatg aaaaaatatc aaacagagcc acataaaaaa
    39481 gcttcaggaa atcttcctgg agttttttct atggcttcag acgaagtgaa aggaagtgat
    39541 ctgaaggaga tcaaacaaat cttccaaaga ggacaacaaa aaggctcaat catttatcaa
    39601 gatgttatct ctcacgacaa ttcgtatctt gaaaaaatgg gcttgtacga tcccaaaaca
    39661 gataccttag atgaagaagg tttgaaagca gctggaagaa aaatgatgga gacattattc
    39721 gagaaagaac aattgaatga aacaggttac tggatggcaa cgattcaccg taacactaag
    39781 catatccata ttcattttgc agttgttgaa aaagaaaata cgcgtaaacc gtatactgag
    39841 attgaaaatg gtgtgagtta tatcgtaccg aaggggaaga gaagacaagc aacattagac
    39901 gcaatgaaaa ccaattatgt acatgagtta gaacgtttct cttatgaaaa ggatcgaatg
    39961 atcggtatgg agaaacgaaa tttattgaca cgaaaaagtg atttgcgtaa caccttaaca
    40021 aaagtagtaa aagaaccagc acgttacgat cgacacgcca tgaacttatt aaatgaagta
    40081 tataaaaccc tacctgaaaa acgttcggaa tggaattatg gagatgaaaa tcgaaccaag
    40141 ctttcaccat ttacaagggg aaaacttgat gagttaacca aatatgtttt aacaaaagaa
    40201 cctggatatc aagaatatat acagatttca aaagcattga aagaagaagc caaaagcctt
    40261 tatggggaaa gcaaaagaga aacaaagaat gcagaaaaaa acgccctctt tgatttaaaa
    40321 aagcgcacag gaaatgcgat tttaacagaa ctgaagagac aggatagtca aataaaacca
    40381 ttaatagaaa gtgtcaatca gatgcaatct atttacaatg aacgatcctt tcgaacactc
    40441 aaacagttgc caacaaatga atacaagtat agttatcaag aattcgtaaa gcgtaggaaa
    40501 caattccaac aaagagttgt cacgcagcga agcttgcgtc aattaaatcg aacaattaat
    40561 gcacatgaag aaaaatacca cgcaagtaaa gcatatgaag agcaacagcg aagaatccaa
    40621 atggaacaag aaagacaata ggaggaagag aaatgaaaca atatttagat caatggaaag
    40681 tcatagaagg atcactaaaa gaagaacgaa tcgaacaact tcctgattgt ttagaaaaag
    40741 agcatttgtt tcagataagc gaaatgttga gaaacgaaca gtttgatcca aatcaatttt
    40801 tagttgttga gtacccagct actggtgtgt attgttgtaa tcatgtgaag ggtgaaaaat
    40861 attttattat tcaagaatac gaaggtaagt tagcgcctta ttatactact tgggaaataa
    40921 atgaagaggg aatcaataat ttcccatgtg aaagcattga agagtctatt tctctcacgg
    40981 aatgttaaat aaatagagac gtaaaagagt cctgaacgtg attgtccagg actctttttc
    41041 aatgaattgt tttccgccaa ttcactgagt ggtgcgattc aattttatta tatcaaaaga
    41101 aagtgagatt ccaaagtgaa gggaggaaga agaaatgact cagacaacga ttggtgttgt
    41161 ggttgaaaaa agcagggata atctcgtttt tgtgacagaa attcaaacag gtagagcttt
    41221 tgtagtcaca gacaaagctg caaaagcgta tcaaaatggc aatattttaa cgttaaatct
    41281 gactacgaag atgttcgttg atgcagtaga aaattatcca tttgtttaga caaacgatat
    41341 tttacaagga ggaaacggac atggcagaag aaaaagaacc actagataat agtcgtttgg
    41401 gaaagtccaa aagaaaatta gtacggttgc aaaacgaatt aaataatcaa tttcaaaagc
    41461 tatttgatca ccaaaaattg acaaatgggc aaccaatgaa tgacaaacga aatggtcgtt
    41521 catggtttaa ccagcaagaa aaaatagaaa ataaaataca aagtttgaga gaagagatca
    41581 agcaacaaga aaaacaggta gaaaaacttg aaagacaaga agagctaaag gaaatgggtt
    41641 ataacaagta cggtggcctg gacatgacga tcgaaaatat tccaagaatc aaagaagaaa
    41701 tcgaacgctt tgaaaaaggt gaatcgactt tttcagctgc cacgatccga aaataccaac
    41761 gaaaattgga aacattagaa cagctgaaag aaaaatcaga gaaaggaaaa gaaaatcttc
    41821 ttcctgaagt acaagcgatc attgacagcg gacgagtgac gcaatggaaa aagaatccaa
    41881 ctatctattt tctgaaaggc tatcgaaaag tcgctttaga actcaatgaa aacgggggat
    41941 ttgaggaatc taaaaaatac caagccacgt cggaagaaga aaaggcagct gttcgtgaat
    42001 tacttgaaga agctacacct actgattctg tgaaggagaa agaaaccgat catttctatc
    42061 gagtagaatt caacgaaaat gatccagcaa aagggttgaa aagttatggt ggggagatcg
    42121 taacactaga attgatcgat caactcaaaa cactggataa ccaatacgcc gcagaagcag
    42181 gctattataa attctatttt gaagaagtga agaatggaga agtagtcgat cagatacgaa
    42241 tggatctagg ggatggcgtc aatagtaatc accccatcta tttagagtta gctagattgt
    42301 gcgaacaatc acctgaaaag gaacaagaac aaacagtcaa aaatgaacct atttcaaaag
    42361 tacaaaacga gattaaagag acaaaaacaa aacaaaaggg ttatcaaaaa cgaacggcag
    42421 aagagattaa gcaagaagtc aaatcacttt cccaacaggc gcttgatgcg gtgaaaaagc
    42481 atactcattc acctgaagac gtcaaagaac ttttgaattt tatgtctcgt tttccagaac
    42541 gatcctttag aaatcaatta ttgatcgagg aacaatttcc aggcgcaacg gcttgtttag
    42601 gtcgtgccgc gttgaaaaaa gaaggaatct atatcaaaaa aggagaacaa gggaacaaga
    42661 tctttgtcag aaaaacggtc aaaggatttt atgagaaagg acgtggcttt gttcgagaaa
    42721 cagaggctac accagaagat aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa
    42781 aaccttacta cacgattgaa aaagtgtttg atatcacaca aacacaattg aaacctgaag
    42841 actatccgaa aatcttcccg aaccgcgtct ttgattttca attggatcaa acagggcaga
    42901 cagaacttca agttgggatt caagcggtag ccaaaaatat tggaattcaa attcgagata
    42961 tgacagaaag tgaagtctat caaagagaat taggacaagc acgtggggcg tacgttcgta
    43021 acgaatttac tggcgaagaa gaaatcgtca tgaatactcg aaatactcct acgcagcatt
    43081 tagccacaag tattcacgaa ttagcccatg ctagaatgca caaattttca gaattagata
    43141 ctgcaacaaa agaactacag gcagaaatga cctcctatat tgtgtgcaag cattttggga
    43201 tggacacctc ggagaaagct attccttata ttgcgacatg gacgaagaat agtcagacgt
    43261 tggatgataa ggaagctgcg gaacgtggga aaatcatgaa tgatgtcagt cgtgtcgcaa
    43321 atgaattcat tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga
    43381 tccagccagc agaagtgaag caggagcata aaaatactgt agaaacggca gaacaaaaga
    43441 atattgtgcc cgttggctca ctttggatcg acaagaaaga cgggaaagtc ctggaagaag
    43501 acacgggccg aacgttacat ttgaaggatg cggcgtttat tgaagatccg cagcataatc
    43561 aagtgatttt aaaaggaaaa agttatggaa aagagatcca aacggcgtta ccttcagaag
    43621 aatttgcgct aggaaatcca ttccgatttt cacttgagac agtgtcagaa gtggcaaaac
    43681 gaaaaaatga aatctattat gaacaacaat tgtcacaaaa agaacgcgta taaaacaagc
    43741 aagaaagact atctgaaaaa tggatagtct tttttctttt gggaggtttg aaaatgacag
    43801 agaaaaaaat gagtctcatt gatcgatgca agcaaatgga tattgtggat tttgcacgaa
    43861 acaatggttt agcggtggcc aataaaggac gagactatcg cttagaagac catgattcat
    43921 ttgtttttga tcgaagaaaa caacgttttt attggaacag tcaaaatatt agtggcgaca
    43981 ttattgaatt agctaaactt tttttcatag acaaagagat ccaagaccct aagcaacaat
    44041 ttaaagcagc cactgatttt attctaaaga atgaagacaa aacagaacga gtggaaaatc
    44101 ttcattttga gacagaaaag tacaaagatc accccgttga ttatcaacca ctcacagaaa
    44161 aaggaaggaa ttatctgaag gaagaacgaa aactgcccga ttggttgatt gattatgcag
    44221 aaaaagaagg attgattgcc gaactaaagc caaaacatga acggcaaaat ttcttagttc
    44281 gagacgatcg tttggatcat gcggtcgcct ttttatggaa agatccacag acaaaagaga
    44341 cagtaggggc gagttatcaa ggaacgttca ttgattatga acgttttggt gagagaggca
    44401 catataagca tatagataaa aattcgacag cgaatcatgg ctttaaccta aagattggtg
    44461 atcctaaaca gttaaaattt tttgagagta gtattgattt acttagctac gctgcactga
    44521 atcgtgatca gttaaacgat acgtggttag tctctatgga agggttaaaa caccatgtga
    44581 tcagtcatta ttttggtgag gcagtatctg aattgagaaa gaaacaagcc tttcctcaat
    44641 ccatcgagat ttgtgtggat aatgaccgtg caggacatat tttctacgaa aaagaacaat
    44701 taatgggcgc agttgatcca tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta
    44761 atgattggca agtgccaaga gaatacaaag tcatttatga agaagtcgca aaagaagaaa
    44821 aggtcacacc agaagctatt atggccatac acaagacaga aaacaatctg caattaacga
    44881 accaactggt ttccgctcac aaagtcaatg cgtcttttgg tcagcagctt tctgttaacg
    44941 attcaataga agtcattagt ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag
    45001 attgtgaacg ctcgaatggt acgtatgatt ttgatcgttt ttatcaagaa aaaggggata
    45061 ttaatgctca gattcttttc tcttataaag cagaacaata ctacaaaggc tataaaaatc
    45121 atgaacatga atttgtacct gaagtaaaaa aggattggaa tgatcaattg aagcatgaga
    45181 tccatcaaca ggaaattaga aagcaaaaac gtgcgatgtt gtttcaacaa ggcagacaac
    45241 aagaaaggga gtgagatcgt cacgatgaaa actgtcattt tagcagaaaa accgtcacaa
    45301 gcgaaggcct atgccgatag cttttctaaa gccacccgaa aagacgggta ttttgaaatc
    45361 caagatcgat tgttttcggg tgaaactgtg atcacgtatg gttttggtca tttagtcgag
    45421 ttagattcgc ccgatatgta cgatgaaaat tggaaacaat ggtcattaga gcatttgcct
    45481 attttcccta cacattatca ctatcatgtt cctaaagata agaaaaagca attcaacgtg
    45541 gtgaaacagc aacttcaatc ggccgacacc attattattg cgacagatag cgatcgtgaa
    45601 ggggaactga tcgcttggac gatcattcaa caagcaggcg ctgatcaagg aaaaaccttt
    45661 aaaagacttt ggatcaactc cttagaaaaa gaagccattt atcagggatt ccagcaatta
    45721 cgagacgcag aagagaccta tcccaaattt gaagaagcac aagcacgcca gatcgctgat
    45781 tggttgatcg gcatgaacgg cagcccactg tatagtttac tcttgcagca aaagggcata
    45841 ccaggaagtt tttccttagg cagagtccaa accccaacgc tttacatgat ttatcagctg
    45901 caggaaaaga ttagaaactt ccaaaaagag ccttattttg aagggaaagc ccaagtaacc
    45961 gctcaaaatg gcatatttga tgcgaagctt gatcccaatg aaacccaagc gacgcaagaa
    46021 gcatttgaag cctatttaaa agaaaaaggt gttcaagtag gaaagcagcc aggcacgatt
    46081 cttgaagtcg aaaccgagaa aaaaagcgcg gcaagtccac gtcttttttc tctatccagc
    46141 cttcagtcta aaatgaatca gctcatgaaa gctagtgcca aagatacttt ggaagccatg
    46201 caagggttgt atgaagggaa atatctaagc tatccacgaa cagacacccc ttatattaca
    46261 gaaggagagc atgcgtatct gttggatcat ttagatgaat acaagcattt tttaaaggct
    46321 gaatcattcc ctactcctat tcatacaccg aatagtcggt atgtgaataa taagaaagtc
    46381 caggaacatt acgcgatcat tcctacaaaa acagttatga cagaagcaac gtatgaaaag
    46441 ctgacacctt tacaacaagc gatttatgat caagttttaa agacgacggt ggccatgttt
    46501 gcggaaaaat atacctatga agaaactacg attttgacac aagtccagca gcttcagcta
    46561 aaggcagtcg gaaaagtacc agtagatcta ggttggaaaa agctatttgg caaagagagc
    46621 gacagaaaag agaaggaaga agaaccgcta cttcctaaag taacaaaagg agaaacggtg
    46681 acggtagatc tacaaatctt tgaaaaagaa accaagccac cacaacctta cacggaaggt
    46741 acactaatca ccgccatgaa gacagctggt aaaaccgtgg atagtgaaga agcacaatcc
    46801 atattgaaag aagtggaggg aattggtaca gaagcgacac gagcaaatat tattgaaacc
    46861 ttgaagcaaa aggaatatat caaggtagag aaaaataaac ttgtggtgac gaataaagga
    46921 attttgcttt gtcaggctgt tgaaaaagaa ccgctgttaa caagtgccga aatgaccgcc
    46981 aaatgggaaa gctatcttac aaagattggt gagcaaaaag ggacacaagc tacttttcta
    47041 gcaaatatcc aaaaatttgt ttcccacttg ttggaagtcg tcccaggaca aatccaagcc
    47101 actgattttg gagcaacgtt acaagaggtg aaagtagcca gtgaaaagca agaagcggcg
    47161 cgtcatttag gatcttgtcc aaagtgccag gaaaaagaag tcatacttta tcagaaggtg
    47221 gcgacttgca cgtctgaagc ctgtgatttt aaattgtggg taacgattgc caaaaagaaa
    47281 ctcacagcga cacaactgaa agagatcatt cagaacggcc gaacaagtca accagtcaga
    47341 ggactaaaag gtcagaaagg ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca
    47401 acaggatttg agttttctga aaagaagaag accacctaca aaagaaaagt gagacgtaaa
    47461 acgagttaga attgacggag ggaaaaacga tgagaaatac aacgcataaa gaacaagaaa
    47521 tcgaaaatca aaaagccaaa atacgttcag agcaggagaa aatgcgtgcc agcaaacgaa
    47581 gactacagca attgaagaat caacaacgga ttcaaaaaga acttgaatta gatcgataaa
    47641 tcctcctgtt gttaatatta aactaacatg ttatagttat gttagtttaa tattaacatt
    47701 cgagagggaa agcgatgaaa gataaagtca agtgcatttt tgagaaatac aatggtaatc
    47761 tatccttgaa ggatgctaga aatgaagggt tatcacctat gacgttagat cgtttagccg
    47821 aaaaggacaa aatagaaaaa atagcatcag gtttttatac cttaaaagat agttttgtgg
    47881 atgaattata tctcgctcaa agtatctata ctagaggaat cttctctcac gagacagcgc
    47941 ttgatctttt tcaagtagga acatatatcc ctaaaaaaat taatatgatg tttcctaaag
    48001 gatacaatgt ttcgaaagag aatttagatc agtatgccat tcaaccgcat tacacgaata
    48061 aagaaaactt tgaactggga atcacagagg cagaaagctt ttacggcaat aaaattttcg
    48121 tctatgattt agagcgaacg ttatgtgata tgtggaatcc cagatataaa gcaagcgtag
    48181 aagtaaaaca agaagcatta aaagaatata tgacaagtac cagcagaaat acaaataagc
    48241 taagaagata catgaatatt ttaaaatcac caaaagaaat gactttgtat atgttgccac
    48301 tttattgaga ggagtaaagg tgaatgacac cgacacaatt taaagcgaaa acaagaaatg
    48361 ttgcagctga gaaacattta aacccacaaa ttgtt
//
LOCUS       DACDTH010000014        47948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDTH010000014 DACDTH010000000
VERSION     DACDTH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.014"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(38..1189)
                     /locus_tag="IWK54_000867"
     CDS             complement(38..1189)
                     /locus_tag="IWK54_000867"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9593008.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IWK54_000868"
     CDS             complement(1202..1639)
                     /locus_tag="IWK54_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9593009.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IWK54_000869"
     CDS             complement(1654..3420)
                     /locus_tag="IWK54_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9593010.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IWK54_000870"
     CDS             3684..4361
                     /locus_tag="IWK54_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9593011.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4528..5490
                     /locus_tag="IWK54_000871"
     CDS             4528..5490
                     /locus_tag="IWK54_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9593012.1"
                     /translation="MIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEAT
                     YHYEKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYE
                     NIFSGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAA
                     MLSLVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLF
                     LSTISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVI
                     IGGDIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="IWK54_000872"
     CDS             complement(5690..6130)
                     /locus_tag="IWK54_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9593013.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="IWK54_000873"
     CDS             complement(6141..8174)
                     /locus_tag="IWK54_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9593014.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="IWK54_000874"
     CDS             complement(8176..9879)
                     /locus_tag="IWK54_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9593015.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IWK54_000875"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IWK54_000875"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9593016.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="IWK54_000876"
     CDS             complement(10962..11987)
                     /locus_tag="IWK54_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9593017.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="IWK54_000877"
     CDS             complement(12196..13065)
                     /locus_tag="IWK54_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9593018.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="IWK54_000878"
     CDS             complement(13524..15416)
                     /locus_tag="IWK54_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9593019.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="IWK54_000879"
     CDS             complement(15413..17140)
                     /locus_tag="IWK54_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9593020.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="IWK54_000880"
     CDS             complement(17156..17806)
                     /locus_tag="IWK54_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9593021.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="IWK54_000881"
     CDS             18010..18306
                     /locus_tag="IWK54_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9593022.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18478..19098
                     /locus_tag="IWK54_000882"
     CDS             18478..19098
                     /locus_tag="IWK54_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9593023.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="IWK54_000883"
     CDS             complement(19190..20356)
                     /locus_tag="IWK54_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9593024.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="IWK54_000884"
     CDS             complement(20368..20721)
                     /locus_tag="IWK54_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9593025.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="IWK54_000885"
     CDS             20849..21871
                     /locus_tag="IWK54_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9593026.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22310..22744)
                     /locus_tag="IWK54_000886"
     CDS             complement(22310..22744)
                     /locus_tag="IWK54_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593027.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22854..24713)
                     /locus_tag="IWK54_000887"
     CDS             complement(22854..24713)
                     /locus_tag="IWK54_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9593028.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24831..25991)
                     /locus_tag="IWK54_000888"
     CDS             complement(24831..25991)
                     /locus_tag="IWK54_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP9593029.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26298..27722)
                     /gene="araA"
                     /locus_tag="IWK54_000889"
     CDS             complement(26298..27722)
                     /gene="araA"
                     /locus_tag="IWK54_000889"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9593030.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27738..28436)
                     /locus_tag="IWK54_000890"
     CDS             complement(27738..28436)
                     /locus_tag="IWK54_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9593031.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28393..30054)
                     /locus_tag="IWK54_000891"
     CDS             complement(28393..30054)
                     /locus_tag="IWK54_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9593032.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30272..31672
                     /locus_tag="IWK54_000892"
     CDS             30272..31672
                     /locus_tag="IWK54_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9593033.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31924..33003)
                     /locus_tag="IWK54_000893"
     CDS             complement(31924..33003)
                     /locus_tag="IWK54_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9593034.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33089..34048)
                     /locus_tag="IWK54_000894"
     CDS             complement(33089..34048)
                     /locus_tag="IWK54_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9593035.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34060..35583)
                     /locus_tag="IWK54_000895"
     CDS             complement(34060..35583)
                     /locus_tag="IWK54_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9593036.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35593..36156)
                     /locus_tag="IWK54_000896"
     CDS             complement(35593..36156)
                     /locus_tag="IWK54_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9593037.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36162..37025)
                     /locus_tag="IWK54_000897"
     CDS             complement(36162..37025)
                     /locus_tag="IWK54_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9593038.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37039..37935)
                     /locus_tag="IWK54_000898"
     CDS             complement(37039..37935)
                     /locus_tag="IWK54_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9593039.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38110..39381)
                     /locus_tag="IWK54_000899"
     CDS             complement(38110..39381)
                     /locus_tag="IWK54_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9593040.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39371..40888)
                     /locus_tag="IWK54_000900"
     CDS             complement(39371..40888)
                     /locus_tag="IWK54_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9593041.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41086..41976
                     /locus_tag="IWK54_000901"
     CDS             41086..41976
                     /locus_tag="IWK54_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9593042.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="IWK54_000902"
     CDS             complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="IWK54_000902"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9593043.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42885..44357)
                     /locus_tag="IWK54_000903"
     CDS             complement(42885..44357)
                     /locus_tag="IWK54_000903"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9593044.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44994..45356)
                     /locus_tag="IWK54_000904"
     CDS             complement(44994..45356)
                     /locus_tag="IWK54_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9593045.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45579..46331
                     /locus_tag="IWK54_000905"
     CDS             45579..46331
                     /locus_tag="IWK54_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9593046.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46471..47394)
                     /locus_tag="IWK54_000906"
     CDS             complement(46471..47394)
                     /locus_tag="IWK54_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9593047.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47409..47681)
                     /locus_tag="IWK54_000907"
     CDS             complement(47409..47681)
                     /locus_tag="IWK54_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593048.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13977 a         9953 c         8268 g        15750 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttattata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
    22261 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
    22321 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
    22381 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
    22441 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
    22501 ccattgcttt tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt
    22561 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
    22621 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
    22681 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
    22741 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
    22801 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
    22861 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
    22921 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
    22981 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    23041 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    23101 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    23161 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    23221 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    23281 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    23341 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    23401 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    23461 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    23521 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    23581 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    23641 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    23701 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    23761 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    23821 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    23881 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    23941 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    24001 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    24061 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    24121 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    24181 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    24241 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    24301 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    24361 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    24421 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    24481 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    24541 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    24601 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    24661 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    24721 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    24781 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    24841 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    24901 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    24961 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    25021 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    25081 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    25141 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    25201 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    25261 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    25321 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    25381 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    25441 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    25501 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    25561 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    25621 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    25681 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    25741 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    25801 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    25861 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    25921 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    25981 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    26041 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    26101 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    26161 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    26221 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    26281 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    26341 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    26401 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    26461 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    26521 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    26581 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    26641 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    26701 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    26761 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    26821 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    26881 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    26941 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    27001 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    27061 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    27121 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    27181 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    27241 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    27301 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    27361 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    27421 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    27481 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    27541 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    27601 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    27661 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    27721 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    27781 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    27841 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    27901 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    27961 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    28021 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    28081 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    28141 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    28201 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    28261 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    28321 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    28381 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    28441 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    28501 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    28561 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    28621 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    28681 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    28741 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat
    28801 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    28861 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    28921 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    28981 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    29041 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    29101 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    29161 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    29221 ccctgtccgt ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    29281 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    29341 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    29401 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    29461 gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac
    29521 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    29581 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    29641 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    29701 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    29761 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    29821 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    29881 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    29941 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    30001 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    30061 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    30121 ttattgtata cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac
    30181 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    30241 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    30301 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    30361 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    30421 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    30481 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    30541 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    30601 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    30661 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    30721 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc
    30781 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    30841 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    30901 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    30961 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    31021 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    31081 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    31141 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    31201 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    31261 actttcattc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    31321 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    31381 gacatgggta atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    31441 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    31501 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    31561 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    31621 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    31681 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    31741 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    31801 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    31861 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    31921 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    31981 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    32041 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    32101 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    32161 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    32221 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    32281 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    32341 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    32401 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    32461 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    32521 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    32581 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    32641 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    32701 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    32761 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    32821 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    32881 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    32941 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    33001 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    33061 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    33121 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    33181 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    33241 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag
    33301 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    33361 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    33421 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    33481 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    33541 gattatccat ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg
    33601 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    33661 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    33721 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    33781 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    33841 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    33901 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    33961 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    34021 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    34081 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    34141 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    34201 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    34261 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    34321 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    34381 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    34441 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    34501 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    34561 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    34621 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    34681 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    34741 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    34801 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    34861 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    34921 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    34981 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    35041 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    35101 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    35161 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    35221 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    35281 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    35341 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    35401 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    35461 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    35521 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    35581 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    35641 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    35701 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    35761 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    35821 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    35881 ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa
    35941 gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc
    36001 ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat
    36061 aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat
    36121 caactgtcca taaaatttag tctcaactat tttcatcttc ctcactcttt cattccagac
    36181 atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc
    36241 attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat
    36301 aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa
    36361 agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg
    36421 ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct
    36481 gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat
    36541 gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg
    36601 acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg
    36661 aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga
    36721 gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg
    36781 taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct
    36841 gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc
    36901 agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg
    36961 atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt
    37021 gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt
    37081 ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc
    37141 caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt
    37201 aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat
    37261 cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa
    37321 aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat
    37381 tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat
    37441 gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc
    37501 gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat
    37561 aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg
    37621 attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt
    37681 tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc
    37741 actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt
    37801 caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa
    37861 aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt
    37921 ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt
    37981 ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc
    38041 tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa
    38101 agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt
    38161 ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat
    38221 cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa
    38281 ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc
    38341 aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa
    38401 aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac
    38461 gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt
    38521 ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc
    38581 tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt
    38641 cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc
    38701 aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt
    38761 atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc
    38821 agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc
    38881 taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat
    38941 ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc
    39001 agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc
    39061 tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga
    39121 aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt
    39181 gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc
    39241 agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc
    39301 tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat
    39361 taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca
    39421 cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg
    39481 ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc
    39541 cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta
    39601 gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta
    39661 cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac
    39721 gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat
    39781 agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca ataacgttta
    39841 ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata
    39901 gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa
    39961 tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat
    40021 caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg
    40081 ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat
    40141 catttttttg gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt
    40201 gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc
    40261 ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta
    40321 accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc
    40381 ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac
    40441 tccaataagt tcctttagga aaattacaat attcgactaa attacgagct gcatctactc
    40501 cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga caccagttat
    40561 aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc
    40621 taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc
    40681 caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc
    40741 gaaatccact tttattagct gatggatgat ctggctgata aatcccttca tatactgctc
    40801 gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga
    40861 cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat
    40921 acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt ttttatcgat
    40981 tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa cttttattaa
    41041 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
    41101 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
    41161 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
    41221 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
    41281 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca
    41341 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
    41401 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
    41461 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
    41521 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
    41581 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
    41641 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
    41701 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
    41761 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
    41821 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
    41881 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
    41941 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
    42001 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
    42061 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
    42121 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
    42181 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
    42241 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
    42301 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
    42361 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
    42421 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
    42481 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
    42541 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
    42601 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
    42661 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
    42721 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
    42781 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
    42841 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
    42901 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
    42961 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
    43021 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
    43081 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
    43141 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
    43201 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
    43261 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
    43321 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
    43381 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
    43441 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
    43501 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
    43561 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
    43621 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
    43681 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
    43741 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
    43801 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
    43861 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
    43921 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
    43981 aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt
    44041 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
    44101 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
    44161 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
    44221 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
    44281 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
    44341 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
    44401 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
    44461 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
    44521 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
    44581 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
    44641 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
    44701 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
    44761 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
    44821 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
    44881 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
    44941 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
    45001 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
    45061 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
    45121 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
    45181 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
    45241 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
    45301 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
    45361 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
    45421 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
    45481 taatagtata acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac
    45541 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
    45601 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
    45661 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
    45721 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
    45781 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
    45841 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
    45901 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt
    45961 tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg
    46021 ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa
    46081 ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa
    46141 gcttagcaga ccgtatcttc caagctcata agaagtggtt atcttacagc tggcctgaat
    46201 atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg
    46261 catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa
    46321 aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca
    46381 aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc
    46441 acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt
    46501 cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag
    46561 atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat
    46621 caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga
    46681 atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt
    46741 ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg
    46801 acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat
    46861 gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc
    46921 tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg
    46981 tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa
    47041 tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt ctttctgata
    47101 gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact
    47161 gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt
    47221 ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac
    47281 cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga
    47341 aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc
    47401 ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca
    47461 aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata
    47521 tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac
    47581 ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa
    47641 ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat
    47701 actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa
    47761 gttttattcc tctttcatta ttaccaaagt ctcttatttt aaattgaata cattataatg
    47821 atactgtaaa tatatcttta ttattagtga atggatattt acttttaata gataaaatat
    47881 tataactatt aaaaataact attttcatta taattacaca caaaaaagag cgtgagacaa
    47941 aagtaaag
//
LOCUS       DACDTH010000015        44850 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDTH010000015 DACDTH010000000
VERSION     DACDTH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.015"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IWK54_000908"
     CDS             212..604
                     /locus_tag="IWK54_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9593049.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IWK54_000909"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IWK54_000909"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9593050.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IWK54_000910"
     CDS             3165..4775
                     /locus_tag="IWK54_000910"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9593051.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IWK54_000911"
     CDS             5067..5936
                     /locus_tag="IWK54_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9593052.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IWK54_000912"
     CDS             6101..7372
                     /locus_tag="IWK54_000912"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9593053.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IWK54_000913"
     CDS             7369..8664
                     /locus_tag="IWK54_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9593054.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IWK54_000914"
     CDS             8763..9026
                     /locus_tag="IWK54_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9593055.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IWK54_000915"
     CDS             9471..9920
                     /locus_tag="IWK54_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9593056.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IWK54_000916"
     CDS             9977..11395
                     /locus_tag="IWK54_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9593057.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IWK54_000917"
     CDS             11395..12255
                     /locus_tag="IWK54_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9593058.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IWK54_000918"
     CDS             12429..13202
                     /locus_tag="IWK54_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9593059.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IWK54_000919"
     CDS             13349..13714
                     /locus_tag="IWK54_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9593060.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IWK54_000920"
     CDS             complement(13862..15070)
                     /locus_tag="IWK54_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9593061.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IWK54_000921"
     CDS             complement(15089..15367)
                     /locus_tag="IWK54_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593062.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IWK54_000922"
     CDS             15560..16216
                     /locus_tag="IWK54_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9593063.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IWK54_000923"
     CDS             complement(16658..17455)
                     /locus_tag="IWK54_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9593064.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IWK54_000924"
     CDS             complement(17452..18636)
                     /locus_tag="IWK54_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9593065.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IWK54_000925"
     CDS             complement(18661..19908)
                     /locus_tag="IWK54_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9593066.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IWK54_000926"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IWK54_000926"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9593067.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IWK54_000927"
     CDS             21888..22202
                     /locus_tag="IWK54_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9593068.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IWK54_000928"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IWK54_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9593069.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IWK54_000929"
     CDS             22887..23531
                     /locus_tag="IWK54_000929"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9593070.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IWK54_000930"
     CDS             23546..23875
                     /locus_tag="IWK54_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593071.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IWK54_000931"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IWK54_000931"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9593072.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IWK54_000932"
     CDS             24863..25687
                     /locus_tag="IWK54_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9593073.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IWK54_000933"
     CDS             25680..26027
                     /locus_tag="IWK54_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9593074.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IWK54_000934"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IWK54_000934"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9593075.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IWK54_000935"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IWK54_000935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9593076.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWK54_000936"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWK54_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9593077.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWK54_000937"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWK54_000937"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9593078.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IWK54_000938"
     CDS             31684..32451
                     /locus_tag="IWK54_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9593079.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IWK54_000939"
     CDS             32441..34483
                     /locus_tag="IWK54_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9593080.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="IWK54_000940"
     CDS             34764..34913
                     /locus_tag="IWK54_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9593081.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="IWK54_000941"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="IWK54_000941"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9593082.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="IWK54_000942"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="IWK54_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9593083.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="IWK54_000943"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="IWK54_000943"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9593084.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="IWK54_000944"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="IWK54_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9593085.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="IWK54_000945"
     CDS             39330..40412
                     /locus_tag="IWK54_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9593086.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="IWK54_000946"
     CDS             40412..41215
                     /locus_tag="IWK54_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9593087.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="IWK54_000947"
     CDS             41285..42025
                     /locus_tag="IWK54_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9593088.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="IWK54_000948"
     CDS             42036..43040
                     /locus_tag="IWK54_000948"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9593089.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44848)
                     /locus_tag="IWK54_000949"
     CDS             complement(43085..44848)
                     /locus_tag="IWK54_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593090.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14416 a         7827 c         9341 g        13266 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg
//
LOCUS       DACDTH010000016        43038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDTH010000016 DACDTH010000000
VERSION     DACDTH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.016"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWK54_000950"
     CDS             591..1280
                     /locus_tag="IWK54_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9593091.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWK54_000951"
     CDS             1267..2475
                     /locus_tag="IWK54_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9593092.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWK54_000952"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWK54_000952"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9593093.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWK54_000953"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWK54_000953"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9593094.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IWK54_000954"
     CDS             complement(6578..7273)
                     /locus_tag="IWK54_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9593095.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWK54_000955"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWK54_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9593096.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IWK54_000956"
     CDS             complement(8472..8663)
                     /locus_tag="IWK54_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9593097.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IWK54_000957"
     CDS             complement(8684..9574)
                     /locus_tag="IWK54_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9593098.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IWK54_000958"
     CDS             complement(9561..10328)
                     /locus_tag="IWK54_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9593099.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWK54_000959"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWK54_000959"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9593100.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWK54_000960"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWK54_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9593101.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWK54_000961"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWK54_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9593102.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IWK54_000962"
     CDS             14944..15282
                     /locus_tag="IWK54_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593103.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IWK54_000963"
     CDS             complement(15450..16208)
                     /locus_tag="IWK54_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9593104.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IWK54_000964"
     CDS             complement(16226..17044)
                     /locus_tag="IWK54_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9593105.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWK54_000965"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWK54_000965"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9593106.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWK54_000966"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWK54_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9593107.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IWK54_000967"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IWK54_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9593108.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IWK54_000968"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IWK54_000968"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9593109.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IWK54_000969"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IWK54_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9593110.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IWK54_000970"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IWK54_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9593111.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IWK54_000971"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IWK54_000971"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9593112.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IWK54_000972"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IWK54_000972"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9593113.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IWK54_000973"
     CDS             27086..27385
                     /locus_tag="IWK54_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9593114.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IWK54_000974"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IWK54_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9593115.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IWK54_000975"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IWK54_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9593116.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IWK54_000976"
     CDS             28352..30325
                     /locus_tag="IWK54_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP9593117.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IWK54_000977"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IWK54_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9593118.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IWK54_000978"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IWK54_000978"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9593119.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IWK54_000979"
     CDS             33204..34496
                     /locus_tag="IWK54_000979"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9593120.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35232..36095
                     /locus_tag="IWK54_000980"
     CDS             35232..36095
                     /locus_tag="IWK54_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9593121.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36224..37846)
                     /locus_tag="IWK54_000981"
     CDS             complement(36224..37846)
                     /locus_tag="IWK54_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9593122.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38095..38616
                     /locus_tag="IWK54_000982"
     CDS             38095..38616
                     /locus_tag="IWK54_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593123.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IWK54_000983"
     CDS             complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IWK54_000983"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9593124.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40697..41515
                     /locus_tag="IWK54_000984"
     CDS             40697..41515
                     /locus_tag="IWK54_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9593125.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41618..41815)
                     /locus_tag="IWK54_000985"
     CDS             complement(41618..41815)
                     /locus_tag="IWK54_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593126.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41984..42562
                     /locus_tag="IWK54_000986"
     CDS             41984..42562
                     /locus_tag="IWK54_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9593127.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT        13082 a         8245 c         8498 g        13213 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    34681 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    34741 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
    34801 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
    34861 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
    34921 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
    34981 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
    35041 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    35101 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
    35161 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
    35221 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
    35281 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
    35341 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
    35401 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
    35461 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
    35521 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
    35581 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
    35641 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
    35701 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
    35761 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
    35821 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
    35881 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
    35941 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
    36001 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
    36061 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
    36121 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
    36181 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
    36241 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
    36301 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
    36361 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
    36421 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
    36481 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
    36541 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
    36601 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
    36661 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
    36721 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
    36781 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
    36841 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
    36901 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
    36961 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
    37021 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
    37081 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
    37141 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
    37201 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
    37261 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
    37321 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
    37381 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
    37441 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
    37501 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
    37561 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
    37621 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
    37681 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
    37741 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
    37801 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
    37861 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
    37921 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
    37981 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
    38041 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
    38101 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
    38161 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
    38221 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
    38281 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
    38341 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
    38401 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
    38461 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
    38521 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
    38581 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
    38641 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
    38701 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
    38761 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
    38821 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
    38881 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
    38941 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
    39001 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
    39061 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
    39121 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
    39181 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
    39241 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
    39301 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
    39361 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
    39421 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
    39481 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
    39541 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
    39601 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
    39661 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
    39721 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
    39781 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
    39841 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
    39901 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
    39961 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
    40021 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
    40081 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
    40141 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
    40201 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
    40261 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
    40321 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
    40381 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
    40441 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
    40501 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
    40561 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
    40621 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
    40681 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
    40741 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
    40801 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
    40861 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
    40921 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
    40981 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
    41041 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
    41101 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
    41161 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
    41221 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
    41281 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
    41341 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
    41401 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
    41461 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
    41521 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
    41581 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
    41641 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
    41701 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
    41761 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
    41821 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
    41881 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
    41941 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
    42001 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
    42061 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
    42121 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
    42181 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
    42241 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
    42301 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
    42361 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
    42421 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
    42481 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
    42541 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
    42601 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
    42661 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
    42721 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
    42781 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
    42841 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
    42901 aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg taagagataa
    42961 aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga tatacaacag
    43021 aaatgaataa atattaac
//
LOCUS       DACDTH010000017        42538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDTH010000017 DACDTH010000000
VERSION     DACDTH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.017"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            138..1406
                     /gene="rseP"
                     /locus_tag="IWK54_000987"
     CDS             138..1406
                     /gene="rseP"
                     /locus_tag="IWK54_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9593128.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            1474..3183
                     /locus_tag="IWK54_000988"
     CDS             1474..3183
                     /locus_tag="IWK54_000988"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9593129.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            3395..7753
                     /locus_tag="IWK54_000989"
     CDS             3395..7753
                     /locus_tag="IWK54_000989"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9593130.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            7898..8371
                     /gene="rimP"
                     /locus_tag="IWK54_000990"
     CDS             7898..8371
                     /gene="rimP"
                     /locus_tag="IWK54_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9593131.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            8394..9569
                     /gene="nusA"
                     /locus_tag="IWK54_000991"
     CDS             8394..9569
                     /gene="nusA"
                     /locus_tag="IWK54_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9593132.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            9591..9884
                     /locus_tag="IWK54_000992"
     CDS             9591..9884
                     /locus_tag="IWK54_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9593133.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            9881..10192
                     /locus_tag="IWK54_000993"
     CDS             9881..10192
                     /locus_tag="IWK54_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9593134.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            10205..12511
                     /gene="infB"
                     /locus_tag="IWK54_000994"
     CDS             10205..12511
                     /gene="infB"
                     /locus_tag="IWK54_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9593135.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            12538..12885
                     /gene="rbfA"
                     /locus_tag="IWK54_000995"
     CDS             12538..12885
                     /gene="rbfA"
                     /locus_tag="IWK54_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9593136.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(13042..13215)
                     /locus_tag="IWK54_000996"
     CDS             complement(13042..13215)
                     /locus_tag="IWK54_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9593137.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            13663..16025
                     /locus_tag="IWK54_000997"
                     /pseudo
     CDS             13663..16025
                     /locus_tag="IWK54_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            16393..17316
                     /gene="truB"
                     /locus_tag="IWK54_000998"
     CDS             16393..17316
                     /gene="truB"
                     /locus_tag="IWK54_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9593138.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            17320..18261
                     /gene="ribF"
                     /locus_tag="IWK54_000999"
     CDS             17320..18261
                     /gene="ribF"
                     /locus_tag="IWK54_000999"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9593139.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            18613..19398
                     /locus_tag="IWK54_001000"
     CDS             18613..19398
                     /locus_tag="IWK54_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9593140.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            19411..20262
                     /locus_tag="IWK54_001001"
     CDS             19411..20262
                     /locus_tag="IWK54_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9593141.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(20386..20838)
                     /locus_tag="IWK54_001002"
     CDS             complement(20386..20838)
                     /locus_tag="IWK54_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9593142.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(21303..21671)
                     /locus_tag="IWK54_001003"
     CDS             complement(21303..21671)
                     /locus_tag="IWK54_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9593143.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            21821..22303
                     /locus_tag="IWK54_001004"
     CDS             21821..22303
                     /locus_tag="IWK54_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9593144.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            22340..23350
                     /locus_tag="IWK54_001005"
     CDS             22340..23350
                     /locus_tag="IWK54_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9593145.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(23465..24178)
                     /locus_tag="IWK54_001006"
     CDS             complement(23465..24178)
                     /locus_tag="IWK54_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593146.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24368..24958)
                     /locus_tag="IWK54_001007"
     CDS             complement(24368..24958)
                     /locus_tag="IWK54_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9593147.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            25442..25669
                     /locus_tag="IWK54_001008"
     CDS             25442..25669
                     /locus_tag="IWK54_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9593148.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            25685..26851
                     /locus_tag="IWK54_001009"
     CDS             25685..26851
                     /locus_tag="IWK54_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9593149.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            27085..28128
                     /gene="hrcA"
                     /locus_tag="IWK54_001010"
     CDS             27085..28128
                     /gene="hrcA"
                     /locus_tag="IWK54_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9593150.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            28222..28785
                     /gene="grpE"
                     /locus_tag="IWK54_001011"
     CDS             28222..28785
                     /gene="grpE"
                     /locus_tag="IWK54_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9593151.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            28836..30665
                     /gene="dnaK"
                     /locus_tag="IWK54_001012"
     CDS             28836..30665
                     /gene="dnaK"
                     /locus_tag="IWK54_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9593152.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            30816..31982
                     /gene="dnaJ"
                     /locus_tag="IWK54_001013"
     CDS             30816..31982
                     /gene="dnaJ"
                     /locus_tag="IWK54_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9593153.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(32064..32729)
                     /locus_tag="IWK54_001014"
     CDS             complement(32064..32729)
                     /locus_tag="IWK54_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP9593154.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            32705..32878
                     /locus_tag="IWK54_001015"
     CDS             32705..32878
                     /locus_tag="IWK54_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9593155.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(32923..33660)
                     /locus_tag="IWK54_001016"
     CDS             complement(32923..33660)
                     /locus_tag="IWK54_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9593156.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(33662..35164)
                     /locus_tag="IWK54_001017"
     CDS             complement(33662..35164)
                     /locus_tag="IWK54_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9593157.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            35423..37417
                     /gene="uvrB"
                     /locus_tag="IWK54_001018"
     CDS             35423..37417
                     /gene="uvrB"
                     /locus_tag="IWK54_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9593158.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            37427..40243
                     /gene="uvrA"
                     /locus_tag="IWK54_001019"
     CDS             37427..40243
                     /gene="uvrA"
                     /locus_tag="IWK54_001019"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9593159.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            40506..41771
                     /locus_tag="IWK54_001020"
     CDS             40506..41771
                     /locus_tag="IWK54_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9593160.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            41775..42506
                     /locus_tag="IWK54_001021"
     CDS             41775..42506
                     /locus_tag="IWK54_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9593161.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
BASE COUNT        14321 a         7391 c         9132 g        11694 t
ORIGIN      
        1 gaatttcata aaagacgcag gtttttattt ttcattatat tgatactatt ggtgtctttg
       61 gtatactatg gtaaaattat agtgttttat cgttgtttta taaaaaacac aaaaaacaag
      121 taaaggagaa aaaagctatg aaaacgattc tgacatttat catcgttttt ggtatattag
      181 tgattgttca tgagtttggt catttcttct ttgcgaaacg atcaggaatc ctcgttcgcg
      241 aatttgctat cggtatgggt ccgaaaattt atgggcatca agcaaaagac gggacaacat
      301 atacgcttcg cctattacct attggcggtt atgttcgaat ggcaggcaat ggggatgatg
      361 aaacagaaat ggcaccagga atgcctcttt ctcttttact aaattctgac ggcatcgtag
      421 agaagataaa tttaagtaag aaaatccaat tgacaaatgc tatcccgatg gaattgagcc
      481 gttatgattt agaagatgag ctgactatca ctggttatgt gaatggagac gaaacagaag
      541 tagtaaccta ccctgttgat cacgatgcga cgattattga aaacgacgga acagaaatca
      601 gaattgcgcc aaaagacgta cagttccaat cggctaaatt gtggcagcgt atgctgacca
      661 actttgctgg acccatgaat aactttattt tggcaatcct gttattcatc attctggcat
      721 ttatgcaagg tggtgttcaa gtgacgaaca ctaatcgcgt aggagagatc atgcctaatg
      781 gagctgcggc agaagcaggc ttaaaagaaa atgatgaggt agtcagtgtg gacggaaaag
      841 aaatccattc atggaatgat ctgacaacag tcattacgaa aaatccggat aaaacattag
      901 actttaagat cgaacgagaa ggtcaggtgc aatctgtcga cgtgacaccg aaatctgtag
      961 agtcaaatgg cgaaaaagtt ggccaacttg gtataaaagc cccaatgaat acaggtttta
     1021 tggataagat cattggaggg acgcgtcaag ctttcagcgg ttcattggaa attttcaaag
     1081 ccttaggatc actatttaca ggtttcagtt tagataaatt aggcggacct gtcatgatgt
     1141 accaattgtc ttcagaagca gcaaatcaag gtgtaacaac tgttattagt ttgatggcgc
     1201 ttctttcaat gaatctcgga atcgtcaatc tgcttccgat tcctgcctta gatggcggga
     1261 aattagtctt gaatattttt gaagggattc gcgggaaacc attaagtcaa gaaaaagaag
     1321 gaatattgac attagctggc tttggatttt taatgttgtt gatggtgcta gttacatgga
     1381 acgatattca acgattcttt ttctagaaaa agttgacaaa tagggtataa taacaaacga
     1441 taactatttt gaggaaataa aggagtatat agaatgagac aatcaaaaat gttgattcca
     1501 acattacgcg aagttccaaa tgatgcagaa gtattaagcc atcaaatctt gctaagagct
     1561 ggatatatcc gccaagtatc agcgggtatt tattcatatc tgccattagc aaatcgtgta
     1621 ttggaaaaac tgaaacgcat tatgcgggaa gaatttgaaa aaatcggagc ggtagaaatg
     1681 ctgatgcctg ccatcttgcc agcagaactt tgggaagaat ccgggcgtta cgaaacatat
     1741 ggaccaaact tgtatcgttt caaagaccgc aatgatcgaa aaatgatcct tggaccaact
     1801 cacgaagaaa cttttactga tttgatgcgt aatgaaatca attcatataa gaaattacct
     1861 ttaaatcttt atcaaatcca acctaaatat cgtgacgaaa aacgacctcg cttcggtttg
     1921 cttcgcggaa gagagtttat catgaaagat gcgtattctt tccatgcaac ggaagaaagt
     1981 ttggacgaga catataaaga ttacgaaaaa gcctacaccg aagtattcaa gcgttgtggc
     2041 ttagaatttc gctcgattat cggagacggt ggcgcaatgg gcgggaaaga ctcaaaagaa
     2101 ttcatggcga tctcagaaat cggggaggat acgatctgtt attcgactga aagtgattat
     2161 gctgcaaact tggagatggc tacaagctac tatgtatcaa aaaaatcgca tgaaacacag
     2221 cttgaattgg agaaagtagc tacacctaac gtaaaatcaa ttgacgaagt agcagatttc
     2281 ttttctgttg aacctcaaaa aatcatcaag tctgtattgt tcattgcgga tgaacaacct
     2341 gtccttgttt tggtacgcgg agaccatgaa gtcaatgacg taaaactgaa aaacttttta
     2401 tctgctgact ttttagaaga agcaacagaa gaagaagcac aaaaatatct aggtgctgag
     2461 tttggttctg tcggaccagt cggtgtttca gatgacgtaa aagtatatgc agaccgttat
     2521 gtccaagatt tagcgaatgc cattactgga gcgaacgaaa caggattcca ctataaaaat
     2581 gtgaatccag aacgcgattt cactgtactt tcttatgaag atcttcgttt tgtacaagaa
     2641 ggagatcctt caccagacaa caatggtgtt cttgccttta ctcgaggaat cgaaatcgga
     2701 catatcttca aaatcggcac tcgttacagt gaatctatgg gagcaactgt actagacgaa
     2761 aatggacgag aaaaatttgt gattatgggc tgttatggta tcggtgtaag tcgtttatta
     2821 tcagcaatcg tagaacaaca ttcagatgaa caaggaatca actggccaaa aggaattgcg
     2881 ccatttgatt tgcatatcgt ccaaatgaat ctaaaagacg aataccaaac aaacctgacg
     2941 aatgaactag aagaagcgat gacaaaagct ggctatgaag tactggttga tgacagaaat
     3001 gaacgcgcag gagtgaaatt cgcggattct gatttgattg gctgtccaat cagaatcact
     3061 gtcggtaaaa aagcagtgga tggaatcgta gaagtgaaaa tcaagaaaac tggcgaaatg
     3121 gttgaagtac gtaaagaaga attggtgaat actttaccaa tcctcttgaa ccaaggcgaa
     3181 taataagcgt taaaacacag aaaataaaaa tagctgaatc agctgttttt gacaatctga
     3241 tgattcgctt ttaaaattgg aactgcgatt tgaatcgcag ttccaatttt ttaatgtaaa
     3301 actcagaaaa aacgagtata ctaaaaggta ctgaaaaaga agaggataag atgcgatcaa
     3361 ttgcattgtt tctcatcaga aagggcgtat atctttgtca gaaaaacgag atttatttga
     3421 aaaactgttg gaacaaatcc aactagagga agcagaaaaa aatcatccac tgcttactgc
     3481 tggtgaaatg gatagtgtag tggtccatcg taaatcgcgt ttatgggaat ttacgctgca
     3541 tttttccaaa attttgccta ttatgctata tcggtcattg actcagcatc tgactatcgc
     3601 ttttaaagat attgcacaag tcaaactgaa tatcttagca gatgaccaga cattcgatga
     3661 acagctcttg caagattact gggcccaagc tttggaaaat cagcaatgcg atacaccact
     3721 agcacagcaa gtattgaaaa cgcaggtacc tgtgataaaa gatcgtaaag tcatcttgcc
     3781 aatcgacagt acaggagcta tttcttattt gaagcaacaa tacttgccat tagtagaaga
     3841 gttgttcgtc agttacggat tcccaaaatt tcgtatcgaa ccagaagtgg atgagcagca
     3901 agcggaacga gtattgaaac tttttgaaga acgcaaacaa gaacaggcag aagcctttat
     3961 gaagcaagca gctgaaagtc tagttgtcca cgaacaaaag aaaaaggaaa gaaaagagca
     4021 atcgccagct cttgaaggac ccatccacat ccagctagga cgaaatattc cagccgatga
     4081 accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac ttgaggggta
     4141 cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga ctttgaaaat
     4201 cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa aagatgaaca
     4261 agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta ttcaagaaga
     4321 tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca aacatacacc
     4381 tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc atagcaatat
     4441 gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag ggaaatgggg
     4501 acaccgagct atcgccatta cagaccacgg tggtgcgcaa gcctttccgg aagcacatag
     4561 tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg tcgtagatga
     4621 tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca catatgtcgt
     4681 atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg agctagccgc
     4741 agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg atccaggaca
     4801 tcctttgtca cgaacaacgg tagatctgac tggtatcacg gatggaatgg ttcgaggatc
     4861 aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata caattttggt
     4921 agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa ggtatggtat
     4981 tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc tgtatcctca
     5041 attcaaacgt ttcggattag gtgtgttatc taaaaaattt ggtgtcagct tggaacagca
     5101 tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct ttgtgaaaga
     5161 ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata tcggagaagg
     5221 agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa atcaggcagg
     5281 actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg aacgcgtccc
     5341 acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg gctcagcttg
     5401 tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg caagaaaccg
     5461 agcaaaattc tacgattata ttgaagttat gccaaaagcg gtctatgcgc ctttgatcga
     5521 gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt tggtagaaat
     5581 cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc tgaacgaaga
     5641 agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc cgttgaatcg
     5701 tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg catttcactt
     5761 tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa tcgcagatat
     5821 ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc caggttcaga
     5881 agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag atccgttacc
     5941 tgagatcatt caaaaacgat taaaaaaaga gttgaattct attaatggta atggcttctc
     6001 ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg gctatctagt
     6061 aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa tcactgaagt
     6121 taatccgtta gcccctcatt attattgtcc agagtgccaa tattccgagt tttttgaaga
     6181 tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat gtggtgcacg
     6241 cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc atggagacaa
     6301 agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc ataactatac
     6361 gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta cagtcgccga
     6421 caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc agtttcgaag
     6481 tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa ccggccagca
     6541 tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta cgccgatcca
     6601 atatccagca gacgatcaaa attcggaatg gaaaacgact cattttgatt ttcactcgat
     6661 tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg ttattcggat
     6721 gcttcaagat ttatcaggga tcgatccaca aacgatccct acggatgacc cggaagtgat
     6781 gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt attccaaaac
     6841 agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgc tagaagaaac
     6901 gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg gtacagacgt
     6961 atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg cggaagtaat
     7021 cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata gcgggatggc
     7081 atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg atgaattgcg
     7141 agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata tcgattcttg
     7201 ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat tgatggctct
     7261 tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat acttttctgt
     7321 acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg tgaaacaagc
     7381 aatgaaagaa atcacggaca aaggattaga tgcttccgta aaagaaaaga atcaactgac
     7441 agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa tgattgattt
     7501 gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg caccatttag
     7561 agctgtccca agtttgggaa ccaacgtggc aaaacaaatc gttgaagcaa gaaaagacgg
     7621 tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa cattgatcga
     7681 atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat tatcgttatt
     7741 cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga gtatggtata
     7801 gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac tctttttaat
     7861 ggaattacgc acttaatttt ttaaggaggc gaaacttttg agtagcgtcg ttgaaacagt
     7921 cacagaaatg gtgacaccga ttttagatga acaaaatttt gaacttgtcg aagtcgaatt
     7981 tgtaaaagaa ggaaaaagct ggttcttacg agtatttatt gacaaagaag gcggtatcga
     8041 tatcgaagaa tgcgcctttg tcagtgagaa actgagtgaa aaacttgatg cagcagatcc
     8101 agatccgatt cctcaagctt atttcctgga ggtatcctcc ccgggagcag agcggccatt
     8161 gaaaaaagaa agcgactatg aaaaggcact tggtgattat gtccatgtct ccttatacca
     8221 accagtagat ggcgaaaaac aatatgaagg atttcttcaa tcatttgatg ctgaacagct
     8281 tacattgaaa atacgcataa aaacacgaga aaaagaattg acttttgacc gaaaaaatat
     8341 tgcaaaagct cgcttagcta tccaatttta atcacggaga ggaatcagaa aaaatgagta
     8401 aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca aaagaaatcg
     8461 tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat ggccaagcac
     8521 aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat gcggtaaaag
     8581 aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat gcacttttaa
     8641 tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca aaagattttg
     8701 ggcggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt gaagcagaaa
     8761 gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa ggaatcgtcg
     8821 aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca gttctttcta
     8881 aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa gtttatgttt
     8941 cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc catccagacc
     9001 tattgcgccg cttatttgag caagaagtac cagaagtcta tgatggaatc gttgaaattg
     9061 tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca aatgatccaa
     9121 atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa gcaatcgtca
     9181 atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca gctgtattca
     9241 ttgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag acaaatccaa
     9301 aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt aaacgcggac
     9361 aaaacgcacg tttagctgct aaattgacta accataaaat cgatatcaaa tcagaatctg
     9421 acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa ctgcatgatg
     9481 aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca ttcaacaatg
     9541 aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag atgaaacaaa
     9601 ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa aaagagatgg
     9661 tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga aaaatgcctg
     9721 gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg gataaaaaaa
     9781 ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa cttcttgact
     9841 atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag ttaatcggca
     9901 aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca cgggtgaaga
     9961 actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg caaccgatgc
    10021 aagcgagaat acaagaaaaa agataaaaga taagagttca tattatgaag ttccttgctt
    10081 tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca aggtattcgg
    10141 aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt aggaaggtga
    10201 ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat ctagtaaaga
    10261 cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg gtgcgattac
    10321 tagcaatgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga aacaacaagc
    10381 tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac gtaaaaaaga
    10441 aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc gcggtcaagg
    10501 gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata cgggaaatac
    10561 cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta ctaacaataa
    10621 caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta atcgttataa
    10681 taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac caccacgcaa
    10741 attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag cagatattgc
    10801 gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc ttggtgtcat
    10861 ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga cggactatgg
    10921 catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat tcttcgaggc
    10981 agaagaagtc gatccagata aattagtctc acgtccgcca gtcgtaacga ttatgggaca
    11041 cgtagaccat gggaaaacaa ctttattgga tacattacgc cattcacgtg taacaagcgg
    11101 cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg atggtaaacc
    11161 aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg cacgtggagc
    11221 aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca tgccacaaac
    11281 agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg ctgtaaataa
    11341 aattgataaa ccaggtgcaa accctcaaca tgttatgcaa gaattaagtg agtatgagtt
    11401 aattcctgaa gcatggggtg gagaaacgat ctttgttgag atctcagcga aattcggcca
    11461 aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag atttaaaagc
    11521 agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata aaggtaaagg
    11581 tcctgtgtca accttacttg tccaagaagg aacacttcat gttggtgacc caatcgttgt
    11641 cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc gcgataaaga
    11701 agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc aagcaggtga
    11761 ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag aacgcgcgaa
    11821 acgtgcatta ttagaaaacc gtgctgccac aagccgtgtg actttggata acttatttga
    11881 aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag atgtacaagg
    11941 ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg tccgtgtaaa
    12001 aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag cagcagccag
    12061 caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag tgcaagctga
    12121 tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg aagaaatcga
    12181 aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg gtcaaatgat
    12241 cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct atgtaacaga
    12301 aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg taatctatga
    12361 aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca aaatgggtta
    12421 tgaatgtggt gcaatgattg aaaaattcaa tgacatcaaa gtagatgacg taattgaagg
    12481 atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc aatcattatg
    12541 gctaactacc gtgaccgccg agtcggtcaa gaaatcttaa aagaagtcaa tgatattttg
    12601 cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt gaaagttacg
    12661 ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc atcagataag
    12721 aaaacagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga gttagggcac
    12781 aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc agttgtctat
    12841 ggcaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa aactcccgca
    12901 aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt attacctaag
    12961 agttccatga ctttagtctt gcggcgtgtc agctaaccaa aaaagagaaa aagaaaagta
    13021 aaaaattcga ctttcttttt tctagtagta tataaatttt tgcatataga taaagatcaa
    13081 gataccgata acggaagcta gattgtaatc tattttagtg acagtcaggt atagccaagt
    13141 aactagaaga tcagtggaac ctgcaccata aaagtttgag tttgtcggtc cgttgacagt
    13201 taaaagaaaa atcacgttga agttattgat atttccgatt acgccgcttc gatttggtca
    13261 cgatccaaat tttggataat ccctgtagaa attagcatag tagctgggat accaattcac
    13321 atattgacaa agataacaga gacttttaca taaaacggat caatcaggaa aggaattgct
    13381 cgatccagcc ccagttttcc agcaatgtgt taactggccc agcgtcattc agcaaattat
    13441 gcatgatgag gagcgaaatg aactgcggaa cagccattgt gataacgaca agttttttgg
    13501 atcaatgtaa aaaacgtcag cgaaaggaaa aatttcgtgg ttttttatac ctgttcattt
    13561 ggctagatat aaaatccaaa caaatgaaca gaagtgctta ttaggtttca attgggagta
    13621 tctgcgctag aataagagta ataatgaaaa ggagtgttat gtatggatta ttctagtaaa
    13681 gaatactttg ataaaatgac ggcttggtgg cgtgctgcta attatctttc tgttgggcaa
    13741 ctttatctta aagataaccc cttactgaga cgtacactga aacccgaaga tgtcaaaaaa
    13801 catccaatcg gtcattgggg aacgattcct ggccaaaatt ttatttatgt ccatttgaac
    13861 cgagtcatca ataaatatga tttgaacatg ttttatattg aagggccagg acacggcgga
    13921 caagtcatgg tatcgaatgc ttatttagat ggaagttaca cagagatcta tccagaagta
    13981 acggaagatg agacaggaat gcaaaaatta ttcaaacgat tttcatttcc tggaggcatt
    14041 gcttctcatg cagcaccaga aacaccaggt tctatccatg aaggcggaga gcttggatac
    14101 tctctttcac atgcagtcgg agctgtatta gacaatcctg aagtaatctc tgctgtcgtg
    14161 attggtgatg gtgaagcaga aaccggtcct ttagctggaa gctggttctc aaatgtcttt
    14221 atcaatccag tgatagatgg agctgtttta ccgatccttc atttaaatgg tgcaaaaata
    14281 gccaacccaa cgatcttggc acgtaaatca gatggagaat tagcaaatta ctttaacgga
    14341 cttggctggg aacctttctt tattgaagga aatgattcag aaaaattaaa tcctgttatg
    14401 gctgaaaaaa tggatcaagc aatcgagaaa attaaatcca tccaaaaaga agctcgcctg
    14461 aagacagcaa cagatgtcgt gatgccaaaa tggccagtat tgatcgtacg cacaccaaaa
    14521 ggatggaccg gtccaaaaga atgggacggg gaaccaattg aaggaacctt ccgagcacac
    14581 caagtaccga ttcctgtaga tcaagaacat atggatcatg cagatgcttt actccgttgg
    14641 ctgaaatcct atgaaccaga aaaattattt gatgcacaag gacgtatttt agaagaaatc
    14701 agagaaatcg cgcctactgg tgaccaaaga atggcaaaaa atcctatcac caatggtgga
    14761 atcgatccaa gaccgcttat tatgccagat tggaaaaaat atactttgca atttgaaaaa
    14821 ccaggctccg ttaaagcaga agatatgacc gaacttggta aattcgttcg cgagatcatt
    14881 gagaaaaatc cagaaaattt ccgtatattt ggaccagatg aaacaaaatc aaaccgattg
    14941 aatcaagttt tcaaaacgac aaatcgccaa tggatggaaa aaatcgaacc agaaaatgat
    15001 gaatggcttt cgccatctgg tcgcgtcatc gacagtcaat tatcggaaca ccaagatgaa
    15061 gggttcttag aagggtacgt tctgactgga cggcatggtt ttttgcaagt tacgaatcat
    15121 tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatgcgg aaatcacatg
    15181 acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca acagtcttcc
    15241 agcaagatca caatggatat agccaccaag atccaggaat attgacacat ttggcagaaa
    15301 agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg ctcgcagtca
    15361 tggataaagc attccgctcg tcagaaaaaa tcaatttgat catctcttcc aaacatccgc
    15421 gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc ttgaaaatca
    15481 ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct gctgcaggaa
    15541 cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa ttcccaaaac
    15601 taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca agtcaagatc
    15661 cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa ccgatcttat
    15721 ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt cataaccatc
    15781 atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt gacatgcgtg
    15841 tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa atcaaaggaa
    15901 gtcaagcaga agattttgcg aaaaaaatgg atcagaaatt acaagaacat caaaactata
    15961 ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa aatcttgacc
    16021 aataaaatga taaaatcgat cccgcaaaat agaatttttt acattgatta agaaactgaa
    16081 aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg ccagtctttt ttttcgtttt
    16141 tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat caaatggtgc
    16201 aaagaaaaaa tcttccattc tattccctcc tttaaaaaag cataacatga tttctattta
    16261 cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt cattatgatg
    16321 tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc aaaaatgaaa
    16381 tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat gacaagtcat
    16441 gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca tgggggtacg
    16501 cttgacccag acgtagacgg tgtgttgccc atttgtattg gaaaagccac aaaagttatc
    16561 gaatatttga cagattctgg gaaaatctat gtaggcgaga taactctagg tttcagtaca
    16621 acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat gtctttatct
    16681 gaaaaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca gataccacca
    16741 atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg caataatgaa
    16801 acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac aagtgaattg
    16861 acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg cgggaaagga
    16921 acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc tgcgcacatg
    16981 tctgatctga cccgaacagc aagtgccggt atgtcccaga aggatgctct gacattagaa
    17041 gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc aatagaaaaa
    17101 ggagtggaac ggttcaaacg agtagatatt actgatgaag tatggcagaa agtaaaaaac
    17161 ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga agaaattgct
    17221 ttgttttata aaggaaaagt tgtcagtatt tatcagccaa acccgaaaga aaaaaacaaa
    17281 ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat caagatcaga
    17341 catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtgct gggatttttc
    17401 gacggcgtgc atatcggaca ccagaaagtc ataaagacag gtatggaaat tgcacaaaaa
    17461 gaaggattga aattagcatt gatgactttc aatcagcatc catctattgt ctttaaaaag
    17521 atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga aaaaatggcc
    17581 gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc tcatttagca
    17641 ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc tgtttccggt
    17701 tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc tgaatatgcc
    17761 aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga aaaaatcagc
    17821 tctacccgta ttcgtggatt actagacagc ggacaagtaa aagaagcagc aaaactatta
    17881 ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg cagactacta
    17941 ggattcccta cagcaaatgt caaagtaaag agcacggttc atttgcctaa agaaggtgtt
    18001 tatgtgtcgg aaataaaaat tggggaaaca tggtatccag ccatgggttc aattggacat
    18061 aatgatacat tcggtgaagg acgggagctg actgtagagt tgtatatttt ggactttaat
    18121 caagatatct atggtgaaca tgtcgcggta cgctggaatc aatttttaag agatcaagtg
    18181 aaattttcta gtgtagagga attgattgaa caactcaagg aggatgaaca agcaacagct
    18241 gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg tataactagc
    18301 aattacaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa aagtcttgtc
    18361 acagcctttt tttccaaata aatggttttt cagaagtagc ttcttcggaa ataagccgaa
    18421 atttcttgaa gctgaccact tctgtcacaa cctctttctt tatagaaata aaaggacact
    18481 agcaattcat gaaaacgatt gctagagcgt atcatttgct ttagactata tatcttggtg
    18541 tacactcact ttaaggcgga aataaaagtt atccgtaata acaggtttag gtatgaaaaa
    18601 ggagtgggta ctatgtacaa ggaattagaa aacaaagtcg cagtagtaac tggtggctca
    18661 aaggggattg gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa tgtagttgtc
    18721 aactataact cggataaaga aggagcagaa aaagcagtca aagcaatcga agaggcaggc
    18781 ggaaaagcag tctctgtaca agcagatgtc agttctgaag aaggaataca gcgtttactt
    18841 gatacggctt tagaaaattt tgggacactg gacgtttggg ttaataatgc tgggatggaa
    18901 aatcagcatc caacccatga acttagcctt gaagattggg aaagagtgtt aaaagtcaat
    18961 ttgactggcg tttttatcgg aacgaaagcg gctttaaatt actttacaga gaataataaa
    19021 aaaggaaata tcatcaatat gtcttctgtt catgaacaga tcccatggcc aacgtttgct
    19081 cattacgctg cttcaaaagg cggggtaaaa ttatttaccg aaacggtagc aatggagtat
    19141 gcaccgaagg gaatcagaat caatagtata ggccctggtg ctatccgtac acctattaat
    19201 gcggcaaaat ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat cccactagga
    19261 cgcgtaggtg aaccaaaaga aatagcggcg acagctgcat ggttggcttc agacgaatcc
    19321 agttatgtta caggtattac actatttgca gatggtggga tgactttata tccttctttc
    19381 caaggtggaa aaggataagg gtgaataaaa atgggtttat tgattgcatt gatcccggca
    19441 gttggctggg ggatccagcc actgattttg aaaaagattg gtggcagacc aacaaatgag
    19501 atattaggaa ctggtattgg agcggtgctt gtcggcttac tcgttcaatt attcgcttct
    19561 ccagctggta tttcagtgac tacatttttg attagtttac tttctggtgc attttgggtt
    19621 attggacaaa caggacagta tacatctttc aacttgattg gtgtttctaa aacgatgcca
    19681 atttcgactg cattacagtt agtcggaacc tcgttgattg gtgtcttcgc ttttggagaa
    19741 tggtctggaa gtatggggaa aatcattgga gcagcagcaa tcattcttct tgtatttggt
    19801 tctgctttaa cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac aaaaggaatt
    19861 agtctattag catcaacaag tattggttat tgggtataca gtgcattgcc taaactagtt
    19921 gatgcaagcg gactttccat tttctttccg caaatgatcg gtgtcttttt aggggcagtg
    19981 atttatgtga ttttccgtaa accaaccgca ttttctgaag acaagagttg gaaagcaacg
    20041 atcgttggag ccatctttag tgtgtcagca ttggcttata ttttttcagc aaatgaaaat
    20101 ggggttgcta ctgcatatat cattacacag ctaaacgtag ttatctctac attaggcggt
    20161 atgtacatcc tgcatgaaaa taagacacca aaagaaaaaa aattaacgat tatcggatta
    20221 ttgctgatcg tagtaggaag catggtcacg gtctttattt gaggataaag atggtaaaat
    20281 ttcatttatt aaaaaaatat ttgtttgaac caaacagtaa gtacttataa aaaaatagcg
    20341 ctagaatttc caacatctct ggaatttcta gcgcttttta attgtttaag atacaaggga
    20401 aatatctgtc tggatcaata atttttcatt gatccattga ctaaagttta cgacataatt
    20461 gaagttacca ccgattagta tctccgcatg attgtcttta taaagatcgt attgtaaaag
    20521 acggtctttt ttatttgcgt aaacatcgtt atggtactgg actaattgtt ctcggaattg
    20581 attgattgct gtaaaatcac gatattgaag gttgaaacgc atcgtgattg gtgcatttag
    20641 catgaatcgc tgcatttctt ttgctgcttc ctctgcttga tctccgtata ggaactcaaa
    20701 aagattacgt tcagaaaatt tccattgccc gccaaccttt gttcctttta gttttccttc
    20761 atgaagataa tttctgatag aacgtgtagt aacacctaat tgttcggcta attcatttac
    20821 tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg gttctactga cttagttgat
    20881 atttgcttaa tcacgaaatg tagaaagaac gattttaatt aaggtcattg taaaaatcaa
    20941 gtcagaaaat tctaactttt atttctctaa agaaattcgt tctctcctaa agtgatatta
    21001 tatttaaata tacctgaaaa taatgggcag tattatgaaa gatcactgat attttctcgt
    21061 attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactatgta
    21121 gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa
    21181 atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa
    21241 caaaacctgt ataaggtgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag
    21301 ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt
    21361 tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat
    21421 acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg
    21481 atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac
    21541 ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca
    21601 aaaaggtctt tgatcttttg ttgatgttct ttttctttga tttgattgat gatcggctga
    21661 aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa
    21721 tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat atttatatga
    21781 taagatgtct caaataataa aatagaaaag agacataaaa atgattgact tggcaaagaa
    21841 attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt atacaaatga
    21901 agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca aaagcttatt
    21961 cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga aacaaacgga
    22021 tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat cagaacgcat
    22081 ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg aaaaacagat
    22141 tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg caccaggtag
    22201 accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc agctacttga
    22261 gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta aaatagtgca
    22321 tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg caacgattgg
    22381 ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg aatcagcttt
    22441 aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt ggacgcaaga
    22501 taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga
    22561 tgaaaatatc caaatgatct ttccgacttg gggaggagaa ttattgattg agattctcga
    22621 ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag atattagtac
    22681 gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa ctaaccttat
    22741 tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg tactgaaaac
    22801 aagagaagga gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag tgtggcccca
    22861 tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc aatcagtcag
    22921 cggagatgca gtaaggatga agggacgatt attaggtggt tgtattgata cgatcaggca
    22981 tttaatcggt actccttatg gagatgtcca atcttttaaa agaaagtata atgatggaaa
    23041 gccgatcttg tggtttctag aaaactgtga gctatcgtca gttgatttaa gaagatcact
    23101 tgttcagatg aaactggcag gctggtttga tggcagctcg gggatcatgt tcggtagaag
    23161 tgcggcagag gatttgttag attataaaat gaaggatgta tacgaagatc tgtcaaagga
    23221 attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac aactgacatt
    23281 gattaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg ttttgcaaac
    23341 gtttatttaa aaaatttttt cctgccttgg agtctatcaa agaccaataa atctaaatag
    23401 aaaagaaagt gaaagaagct gacaaaatgc tgtcagcttc tttttgtata acatattaga
    23461 aagtctaatt atctataact gtcacatcga ttaactgttt cacgtcattg ggatttcctg
    23521 taggaatagc tactacagat gcattatcaa tgatttcttg agttgtttct ctaatgaacg
    23581 ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga taactagtga
    23641 ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat ttcccagtga
    23701 gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg ataggtgttc
    23761 ctgcaggata atgaataaca tgtaattcga aatcacccga tgtatctcta aaagtattac
    23821 ctctaatagc tagccaaaca cgtgcgtact caatttgtag atcagaatat cctgctaacg
    23881 gatctattga ctgagaagac tgtgaggagg ttgtgtctat tgttgaagaa gaaatatttg
    23941 tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta ctgcttgttg
    24001 aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca gctgaactgg
    24061 tttgtctgat tgaacttttg tctgtggtcg cagatggatc actattttct ttactaggag
    24121 acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac tttttcatat
    24181 aaaatacctc attatttttt taataccagt atagcataat cctgaatttt tgaatccgta
    24241 aaaccttgtc tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt
    24301 gacaaataat catcagtaaa attacttgtc acactattga taaggagtat ttaattgaag
    24361 agaatgatta ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt
    24421 atagacgaat cgttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca
    24481 agtctgtgaa ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag
    24541 cttgttgttc tataaaatct tctttattct gataattttc cgattctaac caaggaaatt
    24601 gcgcatttcc ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg
    24661 ccccacaaat agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata
    24721 aatgatgatt ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt
    24781 caggtatttg ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct
    24841 ctgattggat agaagcagtt ttgttttgcc atttctcatt ttggtttagt gtgctagtaa
    24901 tataagcacc ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt
    24961 tcaagctctc ctttactatc tataacaagg acaacagttt taacctacag acattcatta
    25021 aaattgggta gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagatag
    25081 tcaacttaat taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca
    25141 ttataacatt ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat
    25201 attaaaagaa actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc
    25261 actcagacat gaaaacaacc ctagagatat ataactaggt aaccagttta atgaaagaaa
    25321 aaatcatcca agaagtcgat tcttggattt tttagtggat ttgttcgcca aatacgttta
    25381 gtatgcgtat atgagaggtt gtaagcaaag cgttgtggtc tgagcactta aggaggaatc
    25441 tgtgaaaata gcttttcata tttttgcaga ttttgactta atgccgaagg tcagctttgc
    25501 gattaccgtt tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca ataagcaagc
    25561 atttttagag ctaggtttcc ttgattctaa atatgcggct tatttcaatg agttggtgct
    25621 tgaacgcgat tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag gagaaagaat
    25681 ttcaatgata gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca ttcatatccc
    25741 attttgcgag catatctgct attactgtga ttttaataaa gtttttttag aagggcagcc
    25801 agtagatgag tatgtggaaa tgctgctgaa tgaaatgaaa ataatgctag aaagtcatcc
    25861 gacagcgaat ttagaaacac tttacgtagg gggcgggaca ccaacttctt tatctgctaa
    25921 acaacttgat cgactgttat caggtgctag agagatcctg ccttttcaaa atggtaatga
    25981 gtttactgta gaagcgaatc caggtgattt gacccgtgaa aaacttcaag taatgaaaaa
    26041 ctatggcgtc aatcgtttat ccatgggagt gcagacattt gataatcgct tattgaaaaa
    26101 aattggacgt aaacacaccg cagaagatgt gtatcaaact atgcaatttt tagaagcaga
    26161 aaattttacc aatgtaagca tcgatttgat ttatgcatta ccgggacaaa cactagaagg
    26221 atatctggat actttggaaa aagctttggc tttggatctg cctcactatt ctttgtactc
    26281 attgatcctc gaaaacaaaa caatgttcat gaactgggta cgacaaggaa gactcgaact
    26341 gcctgatcag gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa tggaaaagca
    26401 tggacgtcac caatatgaaa taagtaactt tgggctctcc ggacatgaat ctcaacataa
    26461 cctcatgtac tggaacaatg accactattt tgggtttgga gctggtgcta gcggttatct
    26521 tggagatatt cgttaccgca acaaaggacc aatccaacac tatttacgcc cattacgagc
    26581 aaatgaacgc ccggtattgg aagaagaaaa attgacacga aaaaatcaaa tagaagaaga
    26641 aatgttctta ggactaagaa aaaaaacagg gattactaaa aaacatttct atgatcgcta
    26701 tcaaaaaaca attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt
    26761 acttgttaat gaagaagacc gtgtttattt gacaccaaga gggacctttt tggggaacaa
    26821 tgtgtttgag cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt ttatctgaat
    26881 ttaaataaaa aaagttcaaa aatacaataa aggactattg aaacgttgat ataactggat
    26941 ttacatcaat tatttagaat tagcactctt gtgtttagag tgctaaaaaa tggagtcttt
    27001 ctcttgacag gagaagggct tcttgctata tttataagtg gattagcact tagaatatat
    27061 gagtgctaat gagaggtgag gaagatgtta acaaagcgac aaaacgacat tttacgtctg
    27121 atcatccaac actatacgag tagtggcgtg cccgttggtt ccaaaacatt gatgtctgag
    27181 ggagtcaagg ccagctcagc aacgattcgc aatgatatga aagcattaga agatgaagga
    27241 ttgctgttga agactcactc ttcttctggg cggattccat ctgctttagg ctatcgttat
    27301 tacgtggatc atttgttaaa acctgctcgc gtagcagaag atgatttgct gctgattcgt
    27361 cagtctctag gcagagaatt tcatgaaatc aatgagatta ttaagcaatc tgctgagata
    27421 ttatctgagc tgacaagcta tacgactttt tcattgggac ctgaaataaa agaccgccga
    27481 ctgactggct ttcgtatcgt acccttgaat aatcgacaga ttattgctat cgtcgtaaca
    27541 gatatgggga atgtagaaag ccaagtgttc actcttcccg aatatatggg aagtcaggaa
    27601 ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg gagatcctct gatgacggtg
    27661 taccataaac ttcgtacgga gattccgatg attcttcata aatatttcca aacgacagaa
    27721 gggatgtctc agttatttga caccgttctt ggtcatgttt ttgaagataa ggtttatgtc
    27781 agtggacaaa tgaatctctt ggattatgag ccccgtcaag atcttgatca gttcaaatcg
    27841 atgttttctt ttatgaaaga ctcagacgga cttacacaaa tgatcactcc gatggatagc
    27901 aatattcata tcaagattgg tgctgaatta ggcaatgatt tattgcagaa tatgagtatg
    27961 attcaagcaa gttacgagat agatggtcat ggaagaggta caatagcctt gttagggcct
    28021 actagtatgc cttattctcg gatgttcggt ctagtcgatg tttatcgaag agaactagca
    28081 gctaaacttg ctgattacta ccgttcgctt gacttgtcaa actcgtaatg tgaataacga
    28141 agctatttgc agctgatcgt tctctctttc ttagtaagag caatcaaaca gctgactgaa
    28201 taatgaaagg aagactgtat cgtgaaagaa aatcaagaag aactagataa agaaatgaca
    28261 gatgctcagc cagaaccgga aatcgatgtc gaagctgcag aagattcggg tatttcagaa
    28321 gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg agttagaaga aatggaagat
    28381 aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt
    28441 gagcaattac aaaaataccg ttcacaagat ttagccaaaa aattacttcc atcaattgac
    28501 aacctggaac gtgcattagc cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa
    28561 ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa
    28621 atccctgcta aaggagaagc ctttgatcct aatcttcatc aagcagtaca aactgttcca
    28681 gcgactgaag atacaccagc cgatacgatt gtagaagtac tacaagaagg ctacaaatta
    28741 catgatcgcg tgttaagacc aaccatggtc attgttgcac aataattgtg cggatataaa
    28801 acttacactt tcaattaaag aaggagacgt taattatgag taaaattatc ggtattgact
    28861 taggtacgac taactctgca gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta
    28921 acccagaagg aaaccgtaca acaccttctg ttgtatcatt caaaaatgga gaaatccaag
    28981 taggggaagt tgcaaaacgt caagctgtga caaacccaaa tacgatttca tcaatcaaac
    29041 gccatatcgg tgaagcaggt tacaaagtag atgtagaagg taaatcatat acaccacaag
    29101 aaatctcagc aatgatcctt caatatatca aaggattcgc agaagactat ttaggtgaaa
    29161 aagtcgaaaa agcagttatc actgtgccag cttactttaa tgatgcacaa cgtcaagcaa
    29221 caaaagatgc tggtaagatc gctggtctag aagtagaacg tatcgtcaat gaaccaactg
    29281 cagctgctct tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg
    29341 accttggtgg cggtactttt gacgtatcta tccttgaact aggagacggt gtctttgatg
    29401 tattgtcaac agctggcgac aaccatctag gtggggacga ctttgataac aaaatcatcg
    29461 actacatggt agcagaattc aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg
    29521 ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa
    29581 gcacacaaat cagcttgcca tttatcacag caggagaatc tggaccattg catttggaaa
    29641 tgacattgac acgtgcaaaa tttgatgaat taacagctga cttagtagaa cgtacaaaag
    29701 taccagtacg tcaagcgcta aaagatgcag gtttatcaca atcagaaatc gacgaagtta
    29761 ttttagtcgg tggatctact cgtatccctg cagttgtaga agcagtacgt aaagaaacag
    29821 gaaaagaacc aaacaaatca gttaaccctg atgaagtagt tgctatgggt gctgctatcc
    29881 aaggtggcgt gatcactggt gatgtgaaag atgttgtttt acttgacgtt acaccattgt
    29941 cattaggtat cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc aatacaacga
    30001 ttccaacaag taaatcacaa gtattctcaa ctgcggcaga taatcaacca gcagtagaca
    30061 tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta ggaagattcc
    30121 aattgacaga tattccagca gctcctcgtg gtattccaca aatcgaagta acattcgata
    30181 ttgataaaaa cgggattgta aatgtatctg cgaaagatct aggtacacaa aaagaacaaa
    30241 aaatcacgat caaatcttct tcaggtctaa cagacgaaga aatcgaacgt atggtaaaag
    30301 atgcagaagc aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg
    30361 atgtagatgc attgctattc tctgtggaca aaacattgaa agaattagaa ggcaaagtgg
    30421 acgaagaaga agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc gctgttgaag
    30481 caaacaacat cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact
    30541 tgactgttaa actatacgaa caagcggcac aacaacaagc tcaagaaaac ccagaagcag
    30601 ctcaaggcgg agcagatgat gtcgttgatg ctgattttga agaagtcgat ggcgacgata
    30661 aataatcagt tcactgaatt tcgagagaag gccaaagttg ctactttggc tttttctttt
    30721 gaaaaatagt ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga taacaagcga
    30781 cgtgaaaatg atgagaacca ataggaggaa agcctatggc aacaaaacgt gattattatg
    30841 aagtcttagg gctctcaaaa ggagcatcag aagatgaaat aaagaaagct tatcgtaagc
    30901 tttcgaaaaa ataccatcct gacatcaata aagaacctga tgcggaagaa aaattcaaag
    30961 aagtatcaga agcatatgag attttaagtg acccgcaaaa acgcgctgca tatgaccaat
    31021 acgggcatgc aggcacagat ccaaattacg gtgctggtgg agctggtggc ggatttggtg
    31081 gattcggcgg cttctctggt ggcggcttcg gtggttttga agatatcttc gactctttct
    31141 ttggaggagg cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct gatttgcaat
    31201 atacggtcga cttgtctttt gaagaagcga tttttggtgt agaaaaagaa atcaagtata
    31261 atcgggaaga aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc acacaaccaa
    31321 ctacttgtca taaatgtcat ggtgcaggta cgattaatgt agaacgccaa acaccacttg
    31381 gacgagtgat gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa gaaatcaaag
    31441 aaccttgtcc aacctgtcat ggctcaggac atgagaagaa agcacattca gttaaagtga
    31501 atgtaccagc tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa ggagaagctg
    31561 gagaaaacgg cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa gaaagtgata
    31621 tctttgatcg tgaaggttct gaaatttact atgaattgcc tttaagtttt gtacaagcag
    31681 ctttggggga tgaagtccaa gtaccaactg tccatggaga tgtgaaattg aagatccctg
    31741 ctggaacaca gacaggtacg aacttccgtt tacgtggaaa aggcgctccg aagttacgtg
    31801 gtggtgcaac aggcgaccaa catgtcaaag taaaactgat cacaccgaaa aatctaaatg
    31861 aacaacaaag agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac
    31921 agcaagaaga cggtttcttc gataagttta aagatgcttt tggcgggaaa aagcggaagt
    31981 agtttttcta gtaaaaacgc taagtcatta ataattaaaa accagacgaa caatcatatg
    32041 ttcgcctggt ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata
    32101 tgataatcgt catagtttct aggggtataa ccagtgagag aatggaactt gttcaatcga
    32161 tattgcaagg tgtttttatg aataaataat tcttctgagc aatttttgat gcttccgtta
    32221 tttttactga agcatgccaa agtcttcttg aacaaatgta cttcttcttc tgtcaggctt
    32281 ttcaaaatac gttttacata taatgttcgt tgatctgacg ggattgctgg taaaacgatc
    32341 ccgaggtcta aatcttcaaa aaagcggatc tcgttcacag attgatggaa agctatccat
    32401 tcgcaaactt gtttggcatg aaggaaagat tgcggtgtct cttgctctgt ataacaaagc
    32461 gacccaattc caaagtttag ctgtaactga tacattttat taaactttgt ctggatttta
    32521 tcaagcaatg atagcaatga ggctctggaa atccgattga ctaggataat gatttgttgt
    32581 ctatgtacag tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag caactccaat
    32641 aaaggattga tccgttcttg acacaattgc tgattgtcag aaagttgtcc cacaactaca
    32701 taatatggag aagaaaaatc aagctcaatt aagtgagggt ggtatcgact atgtacttga
    32761 tatcatcaat gaaccgatgg tactttctta tgcgatggaa caggtagagc gattgcttgt
    32821 ttgtgcagga aaacaagcga tgcggctgat acgattaggt gagaaaataa gaagataaaa
    32881 atacacggtc ccttcatggc gaccgtgcat ttttggtttt tattaaatat ttaatacttt
    32941 attcaagaaa tcttttgtcc gttcattttg cggattttca aagatttgtt gaggcgttcc
    33001 atcttctaaa aagtttccgt cgtcaatgaa catgacgcga ttggccactt ctttagcgaa
    33061 ccccatttcg tgcgtaacga taaccatcgt cataccttgt tctgctaact ttttcatgac
    33121 gttcaagaca tcaccgacca tttcaggatc gagtgcagaa gtaggctcat caaaaagcat
    33181 gatgtcaggg ttcatcgcta gagcacgagc aatagccaca cgctgttttt gtccaccaga
    33241 aagcatttca ggatacatat cttttttatc ttctagacca actgtctgca gtaatttgat
    33301 tgcttttttc tctgcttctt ctctagattc tttcttcagg tccatcggtg ctaaaatgat
    33361 gttttccaaa atagaaagat gagggaatag gttaaagtgt tggaatacca ttccgatatg
    33421 ttgtctcacc aagttgatat ctgtgctttt atccatcaag tgggcgccgt caataataat
    33481 ttcaccgtca gaaggttctt ctaaacgatt caagcaacgc aagaatgtac tttttcctga
    33541 acctgaaggg ccaatgatac agacgacatc tccttcgttg atggatacat tgatgtcgtt
    33601 taatactgtg ttatcgccgt attttttgac taaatgctca actaagattt tttctgccat
    33661 cttacttcac cttcttttct aaaacttttg ctaattttgt caatgctgtg atcaaaatca
    33721 agtacatgat cgcaatgatc aagtaaacat acgtactttg tgttgtacgt gcaacaatga
    33781 tcttaccagt ttgcagcaat tcgactacac cgattgcgga aatgatcgtt gtgtctttta
    33841 atgagataac gaactgattg acgaatgatg ggatcatgat gcgtatcgct tgcggcaaaa
    33901 tgattttttg cattgtccga ttataagaaa gacctaaact tctggaagct tccatttgtc
    33961 cgacaggaac ggcgttgatc ccgcctcgaa caatctctgc gatataagca cttgcgttaa
    34021 gagtcaaggt gataacacca gcaaggaagt caggaatcgt aaatccaacg ataccaggca
    34081 acccaaagaa aataaagaac gcaagaacca tcattggaat tcctcggatg atatcgacat
    34141 aaatactagc aattgtgcga agagcgcgta ttggtgctac actgaataat ccaaaaatca
    34201 caccgattac tatagccaaa gcaaaggaga tcaaagttag gagaattgtt ctccaaagtc
    34261 ctttcaacaa tgcttgatag ttgttttgga taagtccaag aaatgttgat tcatcagcag
    34321 tggaagactc actggaagtt tctgaaccat ccgctacata ttctttaata atcttatcgt
    34381 attcgcctgt tctttttaat tctttcaatc cttcattgaa catattaaga agctcaggat
    34441 tttgtccttt tttaacggca aatgcatatt ttccgccaga ttcttttttg atcggtgttt
    34501 ccaattcttg tccttgtttg atcgcataac cgattaccgg ataatcatcc atcattgcat
    34561 cgattgcgcc aacttttaac ccgtcgtaca attgatcagc agcatcaaaa tatttgatag
    34621 aatagccata ttcagattca tggtcgctta aaaattcagc actttcggta ccaacttttg
    34681 ctccgactgt ttttcctttc aaatcttcat aagacttgat cgaagtatct ccttttttga
    34741 cagctaattg gatcccgcta tcaaagtaag aatcagaaaa atcatagctt ttctttcgtt
    34801 catctgttac agtcattccg gcaatcattc cgtctgcttg tcctgattcc aaagcttgca
    34861 aagcagcaga gaaaccacgg tagtccattc ggatgttaaa tccttgcagt ttggcgatgt
    34921 tttccatcaa ttttacatcg atgccttcgt actcgccctt tgcgttttga aattcaaatg
    34981 gagcaaaagc agagtcactg gcaattacgt atacgtcttt tttgggctct attttttgca
    35041 ttgtctcgct tgtgcttgac tctgccgttg tagtgttgtc agcttgtgca acatgaccaa
    35101 acgcaaatgt cacaagtcct gtcattaaga aaacaaatga gtgaaataaa tgctttcttt
    35161 tcataatcaa cctcctaaat ttccaatatt ttattattat aggcgaaaag tgctgaatca
    35221 gacaagactg taagctcgaa aaaatgaaaa aaatgcgagt ttctaacatt ttatgttata
    35281 aaacataaca tgtataataa gataaaaaag tggcgaaagt atgttcggtg tggtataatt
    35341 ttttagtaag gaatgccttt gctttccttt tctttttatt ggtcgtaaaa tgaaaaggaa
    35401 tgaatgaaaa ggaggacgaa gaatgattga gagagatacc tcccgacact ttgagcttgt
    35461 ctcgaaatac caaccggctg gtgatcagcc tgaagcgatt aaccaattag tggatggcgt
    35521 agtcggcggg aaaaaagcac aaatcttatt aggtgcgact ggaacaggga aaacatatac
    35581 gatttccaat cttattgaga aagtgaataa accaacactg attattgccc ataacaaaac
    35641 attggctggt cagctgtacg gggaattcaa ggaatttttc cctaacaatg ctgttgaata
    35701 ttttgtttct tattatgatt attatcagcc tgaagcttat gtgccttcaa gcgatacgta
    35761 tatcgaaaaa gattcaagtg taaatgatga gatcgacaaa ttacgacatt cggctactag
    35821 ttcattatta gaaagaaatg atgtgatcgt cattgcatcc gtttcatgta tttttggatt
    35881 aggctcgcct tttgagtacc aaaaacaagt agtttcgatt cgacagggag cagaactaga
    35941 tcgcaatcag ttgattcgtg acttagtcag tatccaattc gaaagaaatg atatagattt
    36001 tcagcgcggt cgttttcgag tgcgcggtga tgtcgtggag atttttccgg cttcaagaga
    36061 tgaacgtgcc ttacgtgtgg agttttttgg agatgaggtc gaacgtatcc gagaagtcaa
    36121 tgcactgaca ggtgaagttt taggagaaac ggaacacgta gcaatcttcc cggcgactca
    36181 ctttgtgacg aatgatgaac atatggagca tgccgtagcg aatatcaaag cggaattaga
    36241 acagcgttta actgtcttgc gaaatgaaaa caaacttctg gaagctcaac gtttagaaca
    36301 gcgaaccaat tacgatattg aaatgatgtt agaaatgggc tacacgtcgg gaattgaaaa
    36361 ttattctcgg catatggacg gacgtaaaga aggtgaaccg ccttatacgc tattggattt
    36421 ctttcctgaa gattttttga tcgtggcgga cgaatcgcac gtaaccatgc ctcaaatcag
    36481 aggaatgtat aatggtgacc gagcgagaaa acagatgcta gtagattacg gtttccgttt
    36541 gccatctgct ttagacaatc ggccacttcg gttagaagag tttgaaaaac atgtcaatca
    36601 gatcatttat gtctctgcga cgccaggacc atatgaacat gaacaaacag ataccgttat
    36661 ccaacaaatc atccgtccga cagggctgct tgacccagtg atcgaagttc gtccaatcat
    36721 gggacaaatc gatgatctag taggcgaaat aaatgaacga gtcgaaaaag accagcgggt
    36781 gtttgtaacg acattgacga aaaaaatggc agaagacctc acagattatt tcaaagaatt
    36841 agggatcaaa gtcaaatatt tgcatagtga tatcaaaacg ctggaacgaa cagaaatcat
    36901 tcgagatctt cgattaggtg aatttgatgt gttagttgga atcaatttgc ttcgtgaagg
    36961 attagacgta cctgaagttt cccttgttgc tatcttggat gcggataaag aaggcttttt
    37021 acgtagtgaa cgttccttag tccaaacgat tggacgagct gctcggaatg aagaaggtaa
    37081 agtaatcatg tacgcagaca aagtaacaga ttctatgcgt ctagcgatgg atgaaacgtc
    37141 aagacgtcgt acgattcagc agaagtacaa tgaagaacat gggatcgttc caaaaacgat
    37201 cattaaagaa atccgagatt tgatttctat tacgaaagaa tcggaagatg atacaaaaga
    37261 agcggtacaa gtctcgtacg aagaaatgac gaaggaagaa aaagacacgt tattgatgaa
    37321 attagaaaaa gaaatgaaag atgcggcaaa agctttggat tttgaaacag ccgcgaatgt
    37381 gcgagatatg attttagaat taaaagcttc aaagtaggag gacaacatgg ctaacgataa
    37441 aattacgatt catggtgcac gtgcccataa cttgaaaaat atagatgtaa cgattccacg
    37501 agataaattc gtggtcgtta caggtttgtc cggttctgga aaaagttccc tagcatttga
    37561 tacgctgtac gctgaagggc agcggagata cgtagagagc ctttctgctt atgctcgtca
    37621 gtttttagga caaatggata agccggacgt agatagcatc gacggattga gtccggcgat
    37681 ttctattgat cagaaaacaa caagtaaaaa tcctcggtcg acagtaggta ctgtgacgga
    37741 aatcaatgac tatttacgct tgttgtttgc acgtgtagga catccgattt gtcccaacga
    37801 tcatatcgag attaccagtc aatcagtaga tcaaatggca gatcaagtaa tggctttgcc
    37861 tgagcgcaca aagatccaag tgttggcacc gatcgtggta aagaaaaaag gacaacataa
    37921 aaaaatattc gaacgaatcc aaaaagaggg atacgtccgc gttcgagtcg atggtgagat
    37981 ctatgattta agtgaagtgc ctgagctgga gaaaaacaaa aaacatgata tcgcgatcgt
    38041 catcgaccgg atcattgtga aagaagggat tcgttctcgc ctatttgatt ctttagaggc
    38101 ggccctgcga ttagcagacg gctatgctct ggttgacgtg atcggtgaag aagaaatgtt
    38161 gtttagcgaa cactatgcct gtccttattg cggatttact gttggcgaac tagaaccaag
    38221 actgttctca ttcaatgcgc cttttggagc ttgtccagac tgtgatggtt taggtgtcaa
    38281 attagaagtc gatcaagatt tagtgattcc agatagaacg aagacactta gtcaaggtgc
    38341 tatcgttcca tggaatccaa tcagctctca gtattatcct caaatgttag cgcaggcttg
    38401 tgaaagtttc ggtatcgatt tagatacacc attcgaagaa ttaccggaag agcatcagga
    38461 gatcatcttg aatgggacag atgatctttt ccatttccat tatgaaaatg attttggtgg
    38521 cgttcgcgat gtagaaacga ctttcgaagg ggtattgaag aatatcgagc gtcgttatca
    38581 tgatacgaac agtgatttca cgcgtgagca aatgcgtctt tatatgacag aattaacttg
    38641 tcagacatgt aaaggttatc gattgaatcc ccaagctcta tctgttcaaa tcaatggaac
    38701 gaatatcggg caaacaaatg agttgtctat tcaaaaagca gtagatttct tctcaaactt
    38761 aacgttatct gaacaagaga aagtaattgc caaaccgatt ttaaaagaag taaatgacag
    38821 actttcattc ttggaaaatg tgggactaga ctatctaact ttgagtcgcg cttccggaac
    38881 attatctggg ggagaagcgc agcgaatcag attggctaca caaattggat cgaatctttc
    38941 tggagttttg tatattttag atgaaccatc gatcggattg catcaacgcg acaacgatcg
    39001 actgattggc tcattaaaga aaatgcgtga tcttggcaac accctcgttg tagtcgaaca
    39061 tgatgaagat acgatgtggg cagcagatta tttgattgac gtcggacctg gggccggaga
    39121 acaaggcgga caaatcgtgg ctgcaggcac tcctgaagaa gtagctaatg atgaaaattc
    39181 tttgacaggg aaatatcttt cagggaaaaa atcaatccct gtaccaaaag aacgccgtaa
    39241 aggtaatggg aaagcaatca agatcactgg tgcttcagaa aacaatctga aaaatattag
    39301 tgttgaattt ccattaggag aatttgtagc agtcacaggt gtgtctggtt ctggaaagag
    39361 tacattggtc aatagtatct taaagaaatc gttagcgcaa aaattaaata agaattctgc
    39421 taagccaggt aaattcaaga caatttccgg ctacgaaagt atcgaaaaga tcatcgatat
    39481 cgatcaaagc ccaatcggcc ggacgccgag aagtaatcca gcgacttata caagtgtatt
    39541 tgatgatatc cgtgggttat ttgctcaaac gaacgaggca aaaatgcggg gttataagaa
    39601 agggcgtttt agtttcaacg taaaaggcgg tcgttgtgaa gcttgtcgcg gggatggaat
    39661 tattaagatc gaaatgcact ttttgcctga tgtctatgtt ccttgtgaag tatgtcatgg
    39721 taaacgatat aattctgaaa cattagaagt gcattacaaa ggaaaaagca ttgctgatat
    39781 tttggaaatg acagtagagg atgctgtaga attcttcaag cacattccaa agattcatcg
    39841 caaactgcaa acgattgttg atgttggctt aggttatgtg acgatggggc aaccagcaac
    39901 gacattgtcc ggtggtgagg cacaacggat gaaacttgcc agtgaattgc acaaaatctc
    39961 taatggaaag aatttctata tactagatga accaacgaca ggacttcata gcgatgacat
    40021 cgcccgcttg ttgcatgtat tacaaagatt agtagatgct ggtaacacag ttttagtgat
    40081 tgaacacaat ctagatgtaa tcaaaacagc agattatatc attgatttag gaccagaagg
    40141 tggagaaggt ggaggaacga tccttgcgac tggaacacca gaagaaatca ttaacgtaaa
    40201 agaaagttat acaggtcact atttgaaaaa aataatggta taaagaggat agatagctga
    40261 gatagttaaa aaaacataaa aaaatgaatt tttgcttgtt ttattaatat cagtgttcta
    40321 gaatcattac atggtagttc tttctacact gattggtata gataaaaact tcctataagt
    40381 tcaatccgtt caaaaaagaa ttgagcaatc ggaggacttt cctttgattg cttttttctt
    40441 tatggtatga tattccttgt gtagttggaa aagaaaaaca agagattatg agtaagtgag
    40501 agataatgat gaacagtatt gaaaatgaag aatttatccc tatattatta ggaagcgaca
    40561 tgaacgttta tggtatggca cgttctttta atgaagcata cggcaaaatc tgccaagcat
    40621 atgcttcgga tcagttagcc cctacccgct acagtaaaat tgtcaatgta gaagttattc
    40681 caggatttga taaagaccct gtatttatcg agacgatgct tcgtttggca aaagaaagat
    40741 attctgataa gagtaaaaaa tatcttttga ttgcttgtgg tgatggatat gcagaattga
    40801 tctcccaaca taaacaagag ctgtcagaat attttatctg tccatatatt gattattctt
    40861 tatttgaaag gctgatcaat aaagtaagct tttatgaagt ttgtgaagaa tatgatcttc
    40921 cgtatcctaa aacattgatc gtcagagaag aaatgttagt aaatggacat ttggaacagg
    40981 aattgccttt tgaattccca gttgctttga aacctgcaaa cagtgtggag tatctttctg
    41041 tccaatttga aggaagaaaa aaagctttta ttttagagac tagagaagag ttcgatttaa
    41101 ttttaggccg gatatacgaa gccggttata aaagtgaaat gattgtacaa gattttatcc
    41161 cgggtgatga tagtaatatg cgtgtgctaa atgcgtatgt agatgaagat catcaagttc
    41221 ggatgatgtg ccttggtcat ccactattag aagatcctac accagcatcg attggtaatt
    41281 acgttgtgat catgccagat tataacgaaa aaatctatca aaccattaag gcatttttgg
    41341 aaaaaatcga atatacaggc tttgccaatt tcgatatgaa gtatgatcct agagatggag
    41401 aatataaatt atttgaaatc aatttgagac aaggtcgtag cagtttcttt gtgacattga
    41461 acggcttgaa tctggctcgt tttgtcacag aagatcgagt gttcaacaaa ccctttgttg
    41521 aaacgaccta tggaacgaat caatcagata aagcaagatt atggatgggt gtgcccaaaa
    41581 aaatcttttt agaatatgct cgggaaaatg aagacaaaaa actagctgag caaatgatca
    41641 aagaaaatcg ttacggtact actgtatttt atgaaaaaga ccgatcaatc aagcgctggt
    41701 tgttgatgaa atatatgttc cataactata ttccaagatt caaaaagtat tttcatgtga
    41761 aagaaggcta gttcatggag aattttttca gtattttagg cggaatgggc acgatggcga
    41821 cagaaagttt tgttcgcttg atcaaccacc gaacgaaagc tacaaaagat caagaatatt
    41881 tgaattatgt cttattcaat catgcaacag ttcctgatcg tacagcttat attttagatc
    41941 gatcagaaga aaatccaatg ccgtttctgc tggatgatat tgagaaacaa aatttattgc
    42001 gaccaaactt cattgtgttg acttgtaaca ccgcacacta cttttttgaa gagttacaag
    42061 cggcaacgga tattcctatc ttgcatatgc caagagaagc agcaaatgaa cttgtccgtc
    42121 agcatacgac agggagagta gcgattctag gaacagaagg aagtatgaaa gctggaatct
    42181 atgaacgcga agtcaaaaat cttggtttcg aaacagtgat tcctgatact gcgcttcaag
    42241 aaaaaatcaa ttacctgatc tatcatgaga tcaaggaatc ggattatttg aatcaagagc
    42301 tctattacga aatcttagag gaagcggtag agcgcctaaa ctgtgaaaaa gtcatcctag
    42361 gttgcacaga attatctttg atgcatgaat ttgctgaaga taatcattac ccagtgatcg
    42421 atgctcaatc tattttagcg gatcgaacga tcgaacgtgc attagctgaa agaagcgagg
    42481 cattggatac agcatcggaa aagtaaaaaa attggatttg gggcaaatat cctaaaag
//
LOCUS       DACDTH010000018        41155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDTH010000018 DACDTH010000000
VERSION     DACDTH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.018"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(441..1223)
                     /locus_tag="IWK54_001022"
     CDS             complement(441..1223)
                     /locus_tag="IWK54_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593162.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1288..2124)
                     /locus_tag="IWK54_001023"
     CDS             complement(1288..2124)
                     /locus_tag="IWK54_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9593163.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2142..3551)
                     /locus_tag="IWK54_001024"
     CDS             complement(2142..3551)
                     /locus_tag="IWK54_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9593164.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3565..5388)
                     /locus_tag="IWK54_001025"
     CDS             complement(3565..5388)
                     /locus_tag="IWK54_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9593165.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6231..6557)
                     /locus_tag="IWK54_001026"
     CDS             complement(6231..6557)
                     /locus_tag="IWK54_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593166.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6603..6965)
                     /locus_tag="IWK54_001027"
     CDS             complement(6603..6965)
                     /locus_tag="IWK54_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9593167.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7002..7217)
                     /locus_tag="IWK54_001028"
     CDS             complement(7002..7217)
                     /locus_tag="IWK54_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9593168.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7344..7679
                     /locus_tag="IWK54_001029"
     CDS             7344..7679
                     /locus_tag="IWK54_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9593169.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7755..9752)
                     /locus_tag="IWK54_001030"
     CDS             complement(7755..9752)
                     /locus_tag="IWK54_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593170.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9850..10125)
                     /locus_tag="IWK54_001031"
     CDS             complement(9850..10125)
                     /locus_tag="IWK54_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9593171.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10357..11091
                     /locus_tag="IWK54_001032"
     CDS             10357..11091
                     /locus_tag="IWK54_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9593172.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11130..12272
                     /locus_tag="IWK54_001033"
     CDS             11130..12272
                     /locus_tag="IWK54_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9593173.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12367..13758)
                     /gene="sufB"
                     /locus_tag="IWK54_001034"
     CDS             complement(12367..13758)
                     /gene="sufB"
                     /locus_tag="IWK54_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9593174.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13763..14233)
                     /locus_tag="IWK54_001035"
     CDS             complement(13763..14233)
                     /locus_tag="IWK54_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9593175.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14220..15455)
                     /locus_tag="IWK54_001036"
     CDS             complement(14220..15455)
                     /locus_tag="IWK54_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9593176.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15452..16738)
                     /gene="sufD"
                     /locus_tag="IWK54_001037"
     CDS             complement(15452..16738)
                     /gene="sufD"
                     /locus_tag="IWK54_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9593177.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16751..17524)
                     /gene="sufC"
                     /locus_tag="IWK54_001038"
     CDS             complement(16751..17524)
                     /gene="sufC"
                     /locus_tag="IWK54_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9593178.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17721..18569)
                     /locus_tag="IWK54_001039"
     CDS             complement(17721..18569)
                     /locus_tag="IWK54_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9593179.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18593..19279)
                     /locus_tag="IWK54_001040"
     CDS             complement(18593..19279)
                     /locus_tag="IWK54_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9593180.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19272..20306)
                     /locus_tag="IWK54_001041"
     CDS             complement(19272..20306)
                     /locus_tag="IWK54_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9593181.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(20701..21039)
                     /locus_tag="IWK54_001042"
     CDS             complement(20701..21039)
                     /locus_tag="IWK54_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9593182.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21041..21397)
                     /locus_tag="IWK54_001043"
     CDS             complement(21041..21397)
                     /locus_tag="IWK54_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9593183.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21403..22590)
                     /locus_tag="IWK54_001044"
     CDS             complement(21403..22590)
                     /locus_tag="IWK54_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9593184.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22781..23626)
                     /locus_tag="IWK54_001045"
     CDS             complement(22781..23626)
                     /locus_tag="IWK54_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9593185.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23860..24825
                     /locus_tag="IWK54_001046"
     CDS             23860..24825
                     /locus_tag="IWK54_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9593186.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25202..26245
                     /locus_tag="IWK54_001047"
     CDS             25202..26245
                     /locus_tag="IWK54_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9593187.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26307..26771)
                     /locus_tag="IWK54_001048"
     CDS             complement(26307..26771)
                     /locus_tag="IWK54_001048"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9593188.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            27017..27964
                     /locus_tag="IWK54_001049"
     CDS             27017..27964
                     /locus_tag="IWK54_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9593189.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28175..30247)
                     /locus_tag="IWK54_001050"
     CDS             complement(28175..30247)
                     /locus_tag="IWK54_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9593190.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30180..30557)
                     /locus_tag="IWK54_001051"
     CDS             complement(30180..30557)
                     /locus_tag="IWK54_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9593191.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30686..31246)
                     /locus_tag="IWK54_001052"
     CDS             complement(30686..31246)
                     /locus_tag="IWK54_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9593192.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31396..32688
                     /locus_tag="IWK54_001053"
     CDS             31396..32688
                     /locus_tag="IWK54_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9593193.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32748..33866)
                     /locus_tag="IWK54_001054"
     CDS             complement(32748..33866)
                     /locus_tag="IWK54_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9593194.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33880..35301)
                     /gene="arcD"
                     /locus_tag="IWK54_001055"
     CDS             complement(33880..35301)
                     /gene="arcD"
                     /locus_tag="IWK54_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9593195.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35547..36668)
                     /gene="mnmA"
                     /locus_tag="IWK54_001056"
     CDS             complement(35547..36668)
                     /gene="mnmA"
                     /locus_tag="IWK54_001056"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9593196.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37072..37671
                     /locus_tag="IWK54_001057"
     CDS             37072..37671
                     /locus_tag="IWK54_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9593197.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37809..39095)
                     /gene="efmA"
                     /locus_tag="IWK54_001058"
     CDS             complement(37809..39095)
                     /gene="efmA"
                     /locus_tag="IWK54_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP9593198.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39366..39710)
                     /locus_tag="IWK54_001059"
     CDS             complement(39366..39710)
                     /locus_tag="IWK54_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9593199.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39847..40983)
                     /locus_tag="IWK54_001060"
     CDS             complement(39847..40983)
                     /locus_tag="IWK54_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9593200.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11846 a         8649 c         7193 g        13467 t
ORIGIN      
        1 aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg aaaggagtct
       61 aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt ctacatagtt
      121 atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt cataaaaaaa
      181 cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca ctaaaatcac
      241 tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc tccgtttcat
      301 ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc ccagtagctt
      361 atcctaagct actggagatg tataagtacg gtcatataac aacggggtag ggtaaataaa
      421 tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt gtaatcagtt
      481 acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag taaccgaagt
      541 ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag attagctaat
      601 aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat cgtgattcct
      661 ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc gatgaatgaa
      721 tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact cttagatgaa
      781 tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt ctcaccggaa
      841 actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt taaaattccc
      901 acgcttttac taataaattc actataacca gttctttcaa tagagacttc aatgtatttc
      961 acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt tacatctaca
     1021 taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt actgtctgat
     1081 atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc ttctggcaag
     1141 ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac attcacctcc
     1201 gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac aagtctttat
     1261 atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca gtgttagata
     1321 aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact ccttgatttt
     1381 caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca aaaaatcatc
     1441 cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta aatgcgtcac
     1501 aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca gatatcctag
     1561 catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca tatttgcttg
     1621 ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt ctggcaactg
     1681 aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa attcttttgg
     1741 gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt tttgactgcg
     1801 ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat tcagcttaaa
     1861 gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta gatcatccgg
     1921 agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg aatctaattt
     1981 aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt gaaatccaat
     2041 ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa ttaaactgtt
     2101 gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa gccatttctt
     2161 gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa ttctttaaga
     2221 ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc ttgatgggta
     2281 ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc atctgttaaa
     2341 gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg gtcattaatt
     2401 cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat taaaataggt
     2461 aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg gtcaactacc
     2521 cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac accttcttct
     2581 cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc atttttgctg
     2641 gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt accggaggat
     2701 aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt caaatagttt
     2761 tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca gcctctcaat
     2821 gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg atacatagcc
     2881 gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata caagtgaatt
     2941 actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt ttttgaaggc
     3001 agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt gttttgctca
     3061 ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac ctgtacatag
     3121 tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc tagaaaataa
     3181 ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag gcattcatca
     3241 attacttgat gataaaaaga aattcccttt tgattggttt tattattttc aatatccata
     3301 attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt aaacagctgt
     3361 atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc taccccttct
     3421 gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata ggcaccctct
     3481 gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc tgaaagctgt
     3541 ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt gttcctttaa
     3601 gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta ccacaatcac
     3661 tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta ataattgtcc
     3721 tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt ttaaattcac
     3781 ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta gaccaatcgc
     3841 atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa tgctgttttc
     3901 ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag catcgttcac
     3961 ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg cttcggctat
     4021 tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag caatgataaa
     4081 ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca ttggattttc
     4141 tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc tgactaaacc
     4201 gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa ctaatggttt
     4261 tttcagcttc aaggttactc catagagtga aggctcagta attcccatta aagcagtgat
     4321 cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg ctacagcaaa
     4381 ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga tcatttcaga
     4441 acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg tacccgtaac
     4501 caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg ctttgccgtt
     4561 aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg gtccaatgac
     4621 aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa agaatttaat
     4681 aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc aaacagtaaa
     4741 gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc ctagaaaaga
     4801 aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa tgccagcaac
     4861 agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg ctaatagtac
     4921 tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag tttgactact
     4981 gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca tcccggcacc
     5041 tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca atcttgaaat
     5101 aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca cttccttttg
     5161 aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt gtccaccgct
     5221 atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg ctttcctttt
     5281 atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca cattttcttt
     5341 tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta ttctctctcc
     5401 ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa gtgttagttt
     5461 gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc tgtaatccaa
     5521 taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct tttcgtttga
     5581 ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc gtatacttac
     5641 tgctaaaata agtatatcta atattaacta taccagtaaa accattccta cataaccagc
     5701 gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac acaacttcaa
     5761 caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa taaacagcat
     5821 tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca gtaagcaata
     5881 gccaatttca atcattccga gatataaagg tatcgataga atgatttggg ctagtattct
     5941 taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt agtaagtatt
     6001 tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt tttctttttc
     6061 atttctttta tattgttata acagtgaata acataaaaat tcccgacaat cattgtaata
     6121 tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact ccctagtgaa
     6181 tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa tcaatttaac
     6241 attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg atagtactca
     6301 atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta tgtgttatct
     6361 ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta ctgttcttgt
     6421 aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc tgtcattttt
     6481 gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa cttcttgttc
     6541 atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc ttatccctct
     6601 tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac ccctgctatc
     6661 atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat aataacagca
     6721 acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat ccaaccaata
     6781 atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat ttcattaatt
     6841 gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt ttcttgtagt
     6901 gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca ttttctacaa
     6961 tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt tactttatta
     7021 aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag catcttcatt
     7081 tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg ctgtgtaaca
     7141 ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta tcggctgaga
     7201 ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa tatgatgtat
     7261 ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa cttgttgtac
     7321 tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc attagagaaa
     7381 agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc tctacacaac
     7441 aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc ttaacagctc
     7501 tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc gtagattatg
     7561 aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt cgaacaattg
     7621 actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg aaagattgaa
     7681 aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa gattaagagc
     7741 tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac tctccgtcca
     7801 tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc tgataatatc
     7861 aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa aaaactacta
     7921 cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag agtgaatcct
     7981 tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt atccccaaca
     8041 ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag atatgtccct
     8101 tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg tttttccttg
     8161 ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc gttaaaacat
     8221 atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc cagaagtgga
     8281 aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc taatcgatag
     8341 acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa cctatttagg
     8401 tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa tcctataaaa
     8461 gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc atagtaagaa
     8521 ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc cttactatca
     8581 tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc ctctagataa
     8641 ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa aagtactttt
     8701 gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc taatagccaa
     8761 ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc aataaaattt
     8821 tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt tttactggta
     8881 aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt tttacttgac
     8941 ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt aaaagacatg
     9001 tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt gtcgtcattt
     9061 tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc tgaatcactt
     9121 tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat atctaacatg
     9181 ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa cattctattc
     9241 gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc gtaaacagca
     9301 accattttac catagtcagt aattgcttct tttaatagaa agacaatctt gtatttttct
     9361 agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg aactaaattg
     9421 atagataaaa ccttttctac gcttaagtat ctactatcat ctttactcaa atctactacc
     9481 ttccccttct catctttaac agtgttatcg acatccagaa taattactct ggtttccaca
     9541 aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga ttgtgatgaa
     9601 tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa acacctatct
     9661 cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc ttgatctaca
     9721 taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata aaattctgtt
     9781 aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt tttagtctta
     9841 tttgtttact caacctttat accgttccat gaaactatca atatcagatt ttttaaatct
     9901 aatgaccgta tcaattttta taacaggtaa cccgtattga gaaacatatt ttctaatcgt
     9961 tcctactgat acacctagat attcacttgc ctgctgatag tccatatact ctagagtgac
    10021 tccagaaata atgtcttcta gcataggctt tagcactgta attatctctt tcacttgttg
    10081 ttcaacttgt atagaaacaa tgctatcaat gatttgtaga gacatatttc cccctccttt
    10141 cgtgattgct tgaacaaatt ttctaagaat tatcttcgta ttttttgctc aaaataaaag
    10201 tattttgtta ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc gacatacaca
    10261 taatacctcg ataaaaaagg ggtgtcaaca gctttgtaaa atttatttgc aacacccctt
    10321 ttttgaggta tactaatttt aggaggtgtg gtcaatatga taaaacgtcc accaataaat
    10381 tatttagaac gaaaaaagat actcggaaca aaaataaaag ctataagaaa gagtaaaaaa
    10441 ttaacacaac cagcatttgg tcttatgatt aacaacggtc aattaattga caaaaaaact
    10501 atctacgaat gggagaaggg aacctattta ccaataccag agcgattatc ccgaattgct
    10561 gatttgggaa atatgagtat agaggagtta gtttgtggca atgtagaaga atatattttg
    10621 ggaattattc tttatagaga tagcattgta ctagatggta taacatttcc agataaaaac
    10681 ctgttccagc atcttcgtca acagttccca ccagtacact ctaatttaga tacatggctg
    10741 gatagatatt ccaaattaga accagagatg caagaattta ttgctaacaa gacttgtaat
    10801 aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa aaatagaaga attattcatc
    10861 aatgctattg tagaagaatt tgacaataac attcttttcc tcactagcag tatagaagaa
    10921 ctactagaaa gaatggtaga tgaatggcta cctattcaat tgaaagacat gtcttatccc
    10981 gaagaagctg tgagagaaat tactgataat ataaacaagc tagaacaaac gatttcatca
    11041 attgggaaaa aatacactaa gaaaaaaatg aagggaggtg ataccatcta gtggtaagta
    11101 ctccttgtaa actaagagga gatgatttaa tggcaaaaat atcaaagtac aaaaagaaaa
    11161 acggtcaagt ggcgtggatg tataaaggac acattgccac cgatccacga actggtgaaa
    11221 aaataaatac tacgagaaga ggttttagta gcaaacaaga agctcaaaaa gattatgacg
    11281 agtacagaca tcaaatatta tacggtgtta aaaagaaagc acctgatatg acatttgaag
    11341 acctgtataa tgaatggata actcatcagc gtaccagtgt taaagcttca acaatcgcaa
    11401 tctcagtaag atacgcaaat aatcaaatac ttccagcatt tggtaagctt aaactatcga
    11461 atatctctgt cccatattgt caaaaagtgg tagatgaatg gcacagtaaa tatgaaagtt
    11521 acgattatat gagaaaacag actgcacaaa ttttacgcta cggggttgca atgcaatata
    11581 ttgacaataa tcctatggaa aaaacacttc ttccacgaaa aaaagagtat gagaaaaatc
    11641 gtaaatttta ctcaaaagag gagcttaata atttactgga tgcttttaaa gattttggta
    11701 acatgaaaca atacgccttc ttcagattat tggcatacac aggtatgaga aagagtgaag
    11761 tactcgcttt acagtggaaa gacatagaca cgtttaataa agaactacat gtcaataaaa
    11821 ctcttgctgt agatgaattt ggtaaagtta taattcaatc tccgaaaaca agagcttcta
    11881 gacgagttat ctctttagat actgagactc tttctatcct caacaactgg aaattacagc
    11941 aaaaagaaga gtatttaaaa cttggataca atactagctc taaagagcaa catgttttta
    12001 caacagttaa aaacactcta tacattccaa acactgttaa tgattggtta agatatattt
    12061 tgaaaaaata taacttgcct cgtattacac ctcatgggtt tagacataca catgctagtt
    12121 tattattaga agcaggagaa tctgtcaagg tagttcaaca acgattaggg catgaaaata
    12181 gtaaagtgac attagacatc tatgcacata ttacaaataa tgcacctaag aaaacaggtc
    12241 aagactttgc tgatatgatg gctcatcagt aaaagagctg gtcaaaatct ggtcatttct
    12301 agtatacagg caaaacaaaa aagctagaac ccttatatat caagggttct agctcctata
    12361 acaggtttac ccaacactgc cttccatctc ataactgatc aatcgattga gttcgactgc
    12421 atattccatt ggaagttctt tggtgaatgg ttcaacaaag cccatgacga tcatttcagt
    12481 tgcttcagat tcggtcaggc ctcggctcat cagatagtag agttgttctt ctgagatttt
    12541 tgagactttg gcttcgtgtt ctaaagagac ttggctgttg tggatctcat tgaatgggat
    12601 cgtatcggat ttcgaccatt cgtccatgat gatcgtgtca cattcgatat gagagatcga
    12661 acccgcactg tttttggcaa aggttacttg tcctcgatag tttacttctc cgccgtcttt
    12721 tgaaattgat ttagagacga tagagctaga tgtatttggt gcattgtgga tcatttttgc
    12781 acccgtatct tggatttggt ttgctcctgc aaaggcgatg gagagcatgg tgcctcgtgc
    12841 accttttcca tctaagtaaa cacttgggta tttcatcgtt gtttttgcac ctaggtttcc
    12901 gtcgatccat tcaacggttg cgccttcgta tgcttttgct cgtttggtta ctaagttgta
    12961 cacattgtct gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt ttcttgtaaa
    13021 gatttctacg atggcggcat gcaggctgtt acttgaataa gtaggtgccg tacaaccttc
    13081 gacatagtgg atgcttgctc cttcgtcaac aatgatcagc gtacgttcga attgccccgt
    13141 gttttccgca ttgatccgga agtacgtttg taatggaaca tcgactttga cgccttttgg
    13201 cacgtaaata aaggtcccac ctgaccatac ggctgaattc agcgctgcta atttgttgtc
    13261 ggttggcggt acaagttttg aaaagtactc tttgaataat tcagggtatt ctttcaaagc
    13321 agagtcagtg tcagtaaaga cgattcctaa cttttcgaat tcatctttca tattatggta
    13381 cacgacttct gattcatatt gtgcggaagc accggctaga taagctcgtt cagcttctgg
    13441 tataccaatc ttttcaaatg tatctttgat tttttctggt acgtcttccc agtcacgagc
    13501 gggtttgtca cttggttttt ggtaatattt gatggcatcg aaatcgatgt ctgaaaggtc
    13561 ggggccccat gtttgcattg gcattttgtt aaatgtttct agagatttca gacggaattc
    13621 aagcatccat tctggttcgc ctttgattct tgacatctca cggatcactt cttctgtcaa
    13681 gccttctcct gtactgaaaa ccggttcgac atcatcgtga aacccgaatt tatattcttc
    13741 taattctggt acgcccatgt tctcactcct cttccttgtg gtaatgatgg aaagctgcgg
    13801 taccatcgtt ttccattgct tttcctagtg ctttccaggc aagtgtcgca catttgatcc
    13861 gagcgggaaa tttagctaca ccaccaagca tagcagcatc gcctaattgg tcgggatctt
    13921 tgacttcttt tccttggaca agctgtagaa agtctacagt taaatgttct gcttcttcta
    13981 ttgattttcc gatgacggcg tctgtcatca tcgaggcact tgccgtactg atggagcagc
    14041 cactgccgtc aaaggcgata tttttgatga ttccgtcttt tacatcgact tgcaattcga
    14101 tcacatctcc acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca gtcaagcttc
    14161 cacgatgatg cggatgggaa gaatgatcca agatcacttg acggtataaa ttgtctaatt
    14221 tagatagtcc catgttggaa aaactccttt gttgccatga ttgcttcaac taaacggtcg
    14281 gcgtcttgcg ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga tacttcaagg
    14341 tacttcaata gcggttgcgc acaatgatga cctgctctga cggctacccc ttccatgtct
    14401 aaagctgtcg cgacatcatg gggatgtaac ccatccaaat tgaatgcaac gactcctgtc
    14461 cgaaaattcg ggtcttgtgg accataaaca gtcaatccat caattgcttg taattttggc
    14521 aacacataag cggctagttc ttcttcatat cgatggatat tgtccatacc gagtttttgc
    14581 aaataatcga ttgcatggcc taatgcgata gcgccggcga tatttggtgt ccctgcttcg
    14641 aatttccaag gtagttcttt ccatgtactg tcgtataatt cgacaaaatc gatcatttcg
    14701 ccaccaaatt caacaggttc catttcttct aaccattttc gtttaccata taaaacacca
    14761 atacctgttg gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca gtctagtgct
    14821 tggacatcaa tggccatgtg cggggcagat tgagcaccat ctactacgat gaccccgccg
    14881 ttttcatgga caagttcagc taattcttga acaggattga agacacctaa gacattggat
    14941 acatgagcaa tggagacgat tttggttttg tccgtaatct ttttcgctgc atcctccata
    15001 tccaggaagc cttcttctgt tagttcgata tatttcaaga cagcacctgt tcgttttgcc
    15061 aactgctgcc acgggataac attagaatga tgttccatgt aagagagcac tatctcgtct
    15121 cctgctttga tatagcggtc gccgaaggtt ctggctaccc aattcaagct tgtcgtcgta
    15181 ccacgagtaa acagcacttc tgctgtttct tttgcatgga tgaacgcccg tactttttct
    15241 cttgcttgtt cataatcatg ggtcgcgcgt tctgctaacg tatggacacc gcgatgaaca
    15301 ttggcgttat cgtgttcgta atagtacgct aatgcctgga gtacttgttt tggcttttgg
    15361 gtcgtcgctg cattatccag ataaactaaa ggttcatcat tgacgtcttg aaataagata
    15421 ggaaaatcat tgcgtagctt tttagcatcg atcatccatt caacttccct tcgattactt
    15481 cgactaattc tttccgaact tgttctacag ggattgctgt taagacagaa ccgaggaatc
    15541 ctcgaatcac taagcgctct gcttcttctt tatgcaatcc tctactcatc agatagtaca
    15601 tttcttctgg atcaactcgt cctacactgg cagcatgtcc agcagttact tcgttttcat
    15661 cgatcaaaag aataggattc gcatcaccac gtgctttatc agaaagcatc aagacacgac
    15721 tttcttgttg ggcatctgca ccttttgccc ctttcaagat gtggccgata ccattaaagg
    15781 tcaatgttcc tttttctcgg atcactccgt gttgcaaaat atgcccgatc gtatgagggg
    15841 ctttattggt tactcgtgta tcgattcctt ggatttgtct gcccgagctg attgccacga
    15901 ttttgacttc tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca gcgatcacat
    15961 gaccgtcatt catgacacca attgcccagt cgacagaagc atctcgtaaa atgtgcccac
    16021 ggcgattcat ataagaagta atattttctc ctaattgatc tacggctgaa tatttgattt
    16081 ttgaaccagc tttagcgatg acttccacga tgatattacc tgatgatttt gctacttgtt
    16141 ctttggttgt ctggaaacgt tccagataag aaaattcact atgttcatct gccacgatca
    16201 acacatgctt gaagaagtgc tcatcggatg cgccatcttg aatgaataag gactcgagtg
    16261 gttcatcgat cacgacattt ttaggcacat acaggaaaat accgctgttc ataaaagcta
    16321 catggagtgc agtcaattgg tcttcgttca tgtttactgc ttttttcata taatattctt
    16381 ggactagttc tggatattcg atcattgcag tgaacaagtc ggtaaaaatg actccttttt
    16441 ctgctaattc cacaggcagt tgttcaaaaa tcgtccatga accttgttgg acaagcacag
    16501 gattgtcttt cattgcatca aatgccggaa tcgtgcctgt ttcactgatg gtttgtgttt
    16561 ctttcacatc aaataacgac cagcgatgga atttcacccg atcgatatgc ggcaatggca
    16621 gttcatctgc tttcgctagt gcgtcttgac ggaaggttct catccaatca ggttcgcctt
    16681 tacttaaaga aaaatcattt accgcgtcaa gataatcgag ccagtttttt tctttcattc
    16741 ttgctccccc ttacgcttct tcttctttat agtcgattcc taattcttta ctgataccag
    16801 cgtagccttc tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg actactcggc
    16861 cttccatcat gatgtgaacg acatcaggtg tgatatagtt caataatcgt tgatagtggg
    16921 tgatgatcaa tgcaccgaaa ttgtctccgc gcatttcatt tactccttta gcaacgactt
    16981 tcaaagcatc gatatccaga cctgaatcga tttcgtctaa aattgcaaat gtaggttcta
    17041 acatcaacaa ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa ccttcgttca
    17101 gataacgttc agccatttct tctggcatat ttagtaaagc cattttttca tctaactttt
    17161 tcaaaaatgc cataacagaa attttgtcgt tttcatcacg tttcgcattg atcgctgcac
    17221 gcatgaattc cgcattcgtg atacctggta tctcacttgg atattgcata gctaaaaata
    17281 agccaagtcg cgcacgttca tctacttcca agtctaaaac atttttcccg tcaaacaaaa
    17341 tttcgccttc tgttacttca tagtttggat tgcccatgat agcggcagat aaggtagatt
    17401 ttcctgtgcc gttaggtccc atgatggcat ggatttctcc ggttttcatt gttaggttta
    17461 cgcctttaag gatttctttt tcctcgatcg acacatgtag attttttatt tctaagacag
    17521 acatgcatta tacccccaaa ttctctttcg ttctcactca ttttagacta attgagaatg
    17581 aagcgcaatg accttcctta ctaaaatcaa atatctttta tttattatta aacaattttg
    17641 atttctaaat tagaataggt ataaaaaaga tgagaaataa gccattacag acttatttct
    17701 catctttttt gttccatacc ttattttgca tcagccttta ctggtttaac agagccgccc
    17761 catttgtctg tgatccattc ttggacttct ttgcggtgaa gcacttcaat cagttttttg
    17821 attttgtcac tgtcttcatc acctttacga gtcgcaataa tattcacgta aggagagttg
    17881 tccttttcaa gcaatacggc atcttctaat ggattcaacc ctgcgccgta agcgaagttt
    17941 gcgttgattg caactaggtc accttcatca ttttggtacg tagaagctaa aagtgatggg
    18001 tcgatcgtat ggttgaactt caagttctta gggttttctt caatatcatc gaatgttgct
    18061 gtttccagat ctactccatc tttgactttg actaagccag catcttgtag aattgtgatg
    18121 atgcgtcccc agtctgattc agaatttgaa gtaatgattg ttgcaccatc ttttaattcg
    18181 gaaacatctt tgactttttt agagtacaag cccattggtt cgatgtggac agcaccggca
    18241 ttgacaaagt catatccttt ttctttgatt tctttgttca aataaggaat atgctggaag
    18301 taattggcat caatgtcgcc ttcttccaaa gattggttcg gtaatacata gtcatcgaat
    18361 ttgacgattt caagatcgat tccttcatct ttcaataatg gtttcacatg ttccaaaatc
    18421 tctgcgtgcg gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc tttatttgct
    18481 gaatctttgc tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag caaaagcgct
    18541 gctgcaaatc caaaaatttt ctttttcata tcctacatcc ccctatatcc tattatcttt
    18601 tgtctgtttt cttagttaag aaatcaccga ttccttgaac gataaatacg attagtaaaa
    18661 taattaaagt cgcaacgaat gtcactgtta gattaccacg ctggtagcct tcctgccaag
    18721 caagacctcc aagtccacct gcgccgatcg cgccggccat cgcagtaaat ccgatcatcg
    18781 agatcgttgt aactgttaat ccagaaatca atgcgggaat actttcaggt atcaagacct
    18841 tccagatgat ttcccagtaa cttgctccca ttgcatctgc tgcttccagt acaccagggc
    18901 tgacttcacg aaaagcaatc tctactagac gggcataaaa tggtgctgcg gataaaacaa
    18961 gagctggaat ggctgcttgc gcacctagca tagtgcctac gattgcttta gtaaatggaa
    19021 tgatcaaaac catcaggatc ataaatggcg ttgaccggaa aatattactg atgatcgata
    19081 cgattccata aatgattcga gcatacggtt tgtttttgcg tccaatagag taaaggatca
    19141 atcccaaaac taatccgatg atcgtcacta agaccattga gattaatgtc atatatagtg
    19201 tatcaaggat agcctgattc atcgtttccg ggttgatttg tttaaaatct agatatgttt
    19261 ctgaaaagct tttatccacg atggatcacc tcaattccta ctccttgttc tttgatgaac
    19321 acttctgctt gatctaattc tttggtttca ccagtcatca agacagtcaa tgaaccaaag
    19381 gaaccttctt tcacttgttt gatctttcca tacacaacgt tgacatcgat gttatagcgt
    19441 cgaatcgctt gggaaatgac tggctcgttg gcatttcttt cgctaaaggt caaagttgcc
    19501 aaacggccgc ttggattttc tttgatgagt tcagctaata gttctgtcga atcagcttct
    19561 ggttcgatgt cttgttgtac aaagcgtttg gttacttctt ttttcggttg tctgaagaca
    19621 gtcaatacgt ctccttcttc tacgactttc cccaactcca tcacagcgac tttgttgcag
    19681 attttgcgga tcacattcat ttcatgtgtg atcaagacga tggtcaaatt caatttctta
    19741 ttgatcgcta acaataagtc taatacttct tctgttgttt gcgggtccaa tgcgctagta
    19801 gcctcatcac aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat accgacgcgt
    19861 tgtttctgtc cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc taacccgact
    19921 aaacgcaaca gttcttctgc ccgttcttta cgtttgctct ttggtacgcc agctaattcc
    19981 agcggcagct ggatattttc aagtacagtt cttgaccata acaggttgaa atgctggaag
    20041 atcataccga ttttctttcg gaatgtacgt aattctttgt tttttaattt tgtaatatct
    20101 tgatcgttta cgatcacttc gccgctcgtg ggaagctcaa gtccattcag gagacggact
    20161 aatgtacttt ttcctgcgcc agaatagccg acgattccat aaatatcccc tttttcaacg
    20221 tttagtgaaa cgtcattgac ggcttggatg tttccgtttt tcccattgaa tgtctttttg
    20281 acattcgtta gttggattaa tgacatgcca ttcacatctt tctcttatga tttttcaaac
    20341 aaaaaagcct tcgccctctt gccttaagca aaaggacgaa agcttgaact tccgtgttac
    20401 caccttgatt taccgttact tcgcaataac agcctcttcc agtacttgga cttagcttct
    20461 ttccgtatac tgtcgcgctg taacgggcac tcccgtagta agtttgctta cgctcgctta
    20521 ctctgctcta agaccatctt ccatctcatt ttcgttaccc attttcagca aacagggctc
    20581 tcttcaaacg tccgtgacat gtactcttct tttcaaagct ttctcgtttc atataggatt
    20641 gcttatgatt atagcaactg gaagaatcag tgtcaacttt tttcaagtga ttttctgatt
    20701 ttataattcg tcaaattctt ttggatcaac atctttgaaa cttaagatcc acgcattcat
    20761 ttcatctgaa tcattcaaga tattgatatc ttcaccgatt ttatcattaa cagaagaaac
    20821 tgtacccgtt agcgggctgg caaattcatt gaccgctttt tccgcttcaa cttcgatcag
    20881 aggttcatct ttttttagcg attgtcctgc ttttggcaga ttggcaaacg tgatgtctcc
    20941 aagttcttct tgcgcttcat ttgtcaaacc aacacagtat tcttttccat taaataagat
    21001 ccaaaggttg tcctttttct tcaaatgctt gcctgccatt ttatatcagt tctccttcat
    21061 aaattttttc attgaatcca attgcaacaa gctggtcctc tcgtaagatc agtggtcgct
    21121 tgatcagcat accatctgtc gataagactt ggctcgcttt tgaagccgta aactcgccta
    21181 cttgatcttt cagtccaagt tcccgatatt tcatcccgct tgtgttgaac aggcgacgga
    21241 ttggtacttg actcgtttct agccaagtct caatcgttga ggccttcggt acttctttga
    21301 tcatatcaac ggtttgataa ctaacgccat gctggtctaa ccacgctttg gcttttttgc
    21361 atgttgaaca tttcgggtac cagtaaaagg tgtacaattt tcttacctcc ttgaagatct
    21421 tgcaggtgct tcattttggt atctcatcga taatatcaac ccgataccga tcatgttccc
    21481 taaaatcgag gatcctccct gactaatgaa aggcaatggg atccctgtta aaggaagtaa
    21541 tccgatattt gctccaatat tctcaaagac atggaacagc agcatcatga tcaaaccgct
    21601 tgcgatatag gcgtaaaatt cattattcgt atcgaaacac acccgaatca tacgatagat
    21661 taaaataaaa tacaagaaaa tgacgaatgc accaccgata aagccgaagt tttcaccgat
    21721 aacggtaaaa atcatatcgg actcacgaac aggcacatag atattgctta cgttgaatcc
    21781 tttaccaaac aagccaccgg taccaattgc caaaagtcct tgggccggct gataactcat
    21841 tcctgacgta tcatggaaag ggtccagcca tgaatcaata cgtgcaaact gataagattt
    21901 gaatccaact ttataaagca agtctcttcc gccttcagtc gtaaccaaga agatcgttcc
    21961 tgctccaacg actactgcca agattactac aggtgcgatg atgcgccaag agattccaga
    22021 catcaggaaa atcccgccaa agatcgcaag aaagacaagc atcgttccaa agtcatcttg
    22081 ggccatgatc aatgctaaaa ccggtattgt cacgatcaag agcttcccaa tcagccaaaa
    22141 gtccgatttc atcgtccgtt cttttacttg cgtattatgt ttagttacga tcagtgccat
    22201 catcaagatg tacgcaatct tcatcaactc tgctggctgg aaagtaaacg caccgaatcg
    22261 aaaccagttt ttagaccctg ttgaagcagc taaattctga tcatagaatt tgagcaagag
    22321 tcccattacc actaacccca aaccataaag gtaaggagtc agtttccaga tccatttgga
    22381 attcaaatgc atgatgacaa cgattgctgc cgctccaact aaataccaga caacttgcat
    22441 ccctacgcct ttcaagactg ctggattgtt cggatcatgg ctaagtgcta catataatga
    22501 gagcagacca atcaaacata acagaaagac tggtaaaatc acgccataat caatacgatt
    22561 atcgttcgaa gcttttaatt ttttttccat gtttcatccc ttttcttaaa aaccatactg
    22621 ctgattatta tagcggtcca gttagataat ggaaagtctt tccatagaca tttgggaaaa
    22681 attaactttc cgtttgtgca gttgcttgag caaatgcagt acaaaagcct actgcttatc
    22741 tttagtgtat catagaactt tttttgatac atgcaattat ctaccagctg agattctcct
    22801 cgctgtgaga agtttctgtg ctttcattgt atcgaaggat ctcttcggca atctctagca
    22861 gctgatcaga aaccagattg gttcgttgga tgaaataaaa atatcgatgg aaattcaacg
    22921 gagtcattgg cacattttct ggaacagagc gcttagacaa aatcgagcga ccgattcctt
    22981 ccttcagcaa cgcaacaatt acatcgttac ttttcacgat catcttctta tcttggatat
    23041 tcatctcttc aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct tctcttacaa
    23101 gccacaattt gctttttggg tccccagcga tgactagctg gtcttccatc aaagagtatc
    23161 tttgtacgcc ggaagtctct aatggctttt cgataaaacc aaaatcggat tcatgtttcg
    23221 ctacttgttc taatgattca tgagaattcg tgatttcgat ctcaaaatgc actttaggaa
    23281 accttttgat cagatgtttc atcaactgag gcaaaagata gacagcaaag gtgtgggaag
    23341 caacgatccg acatacttct tcttcatttt gttcattgag caatttttgc tgagtttctt
    23401 cccattcttc taacaaaacc agtgtccgtt catataataa gtcggcttgc ttagttggaa
    23461 gcatctcttt tcttccgtta cgttgaaaaa gatcgatatt caattcccgc tccaactgtt
    23521 tgatttgatt cgagactgct ggttgagata aaaacagtac ttctgctgct ctggaaaaat
    23581 ttcttgtttc atatactgct cgaaaagtcg ttaataactt aaacatggca ccacttccat
    23641 aactttttat gataattact ataaaaacta tttgatttaa atatacaaaa aaccatctat
    23701 aatagaaatg tacaacaaaa cagttgctga tccaactagt aacttagatg aagacaatca
    23761 gctgtctgct tgctcgtaat catacattcc tattcatttt acaatgtatt cgttcatctt
    23821 ggtactaaaa tctattaaat aaaaaggaga atattatata tgaagaactt tttgaaaatt
    23881 attccgatcc cgatctgcgg gttgatcctt gggttagctt cccttggaaa tctgtttaaa
    23941 gactatcacc atgttgcttt aggcaatcta actggcggta tctctatgat tcttatgata
    24001 ttcatttttg ttaaattgtt tatcttattc gaacacacga aacaaacgtt gcatgatcca
    24061 atcatcgcct ccgtttcacc aacttttacg atgtcactca tggttatttg tacttatttt
    24121 gtttcatatg aaagtattgc accaattgtc aaattcattt ggctgtgcgc tgttatcttc
    24181 caagtgatcc ttgtctttta tttcaattat caccatgtta tcaaagctga tctttcaatt
    24241 gaagcaattt atccaagctg gtttatcatt tacgttggat ttggggtcat cactgtaact
    24301 gccgggaact tcttcccact tattggaaaa attttctttt ggatttcatt gatctgctat
    24361 gcgttcttat tgccgatcat catcaaacgt gtctttttcg taagaaatat ggctcatccg
    24421 acacgtccgc tgattacgat cgttgcagca cctggttctc tttgcctaac aggttatctt
    24481 aaaaactttg ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt atctcaagta
    24541 ttgtatttcg ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt ttatccaagc
    24601 tatgcagcct ttacattccc gcttgttatc tctgcaacag ctatatttac tactgttcat
    24661 tacttcaaca gtttaggtat ctatagtagc gtattggatt tcttaaaagt tgcggaatgt
    24721 ctgtttgcaa gtgtagttgt cttctacgtt cttgctcatt acgtacgttt cttaattaaa
    24781 gaacatagca aacatggaaa atttgtttct aaaaaaatcg cttaataatc gattcaatag
    24841 gttgtgaaaa agctgttctg catcaagtaa taagaacggc caaacaaaaa cagctgttca
    24901 tgtttttgta ctggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaccaa
    24961 gaggtcgtaa aagaagtggt cagcttcaag aaataagaag gaatttacga aaattgcttc
    25021 tcaaattttt gtgaaatttc ggcttatttc cgtagaagct acttctggaa caccgtttat
    25081 tcgaaacaag aggtcgtaca agacttttgt cacgacctct tgttcttttg tcaaaatact
    25141 ggtttagtgc tactatgttc gagtatgctt ttagtattta ttgacgaaag gaaccgatca
    25201 aatgaaatca ttgtcacatc caataaaatc tgtaaaggat tcagcggtcc gcgaaatctt
    25261 acccggttta ctgctttctt ttgccatcgc tgccttcagc aggcttcttg ctatttatct
    25321 gccacagtta ggtggagcga ctattgctat tttattgggt attttattag gaaatacttt
    25381 ttttaaacag cctgttctag caatcggcac aaaatttgct gaaagcaaac tattagaatt
    25441 ctctgttttt ttactaggtg caactgtcac agctcaaacg attgctcaaa ttggtgtaaa
    25501 aggaattgtt tttgtgttac tgcaaatgtc agtcactatc atcggtgctt acttgattgg
    25561 taaaaaactg caattcagcg atacttcttc tttgatgatg gctggaggaa acgctgtttg
    25621 tggttcttct gctattgctt caatcgcccc ttccatccat gcgaaagagg aagaaaaagg
    25681 acagatcatc acactagtca atctactggg tactgtgatg atgttgactc tcccttttct
    25741 tggattacag ttgtttggtg gaaatctcat tgaaaaaagt gcacttttgg gtggtacact
    25801 ccaatctgtt ggtcaagtag tcgctggggc aagcttactt gacaattctg ttgtcgaatt
    25861 cgccatgcta tttaagattc tacgtattat gatgctcgtt gttgttgtct atctgtttga
    25921 aaaattcatg cacaagaaac aaccagctct gtttgaaacg ccaactgata cgcagaaaaa
    25981 acgaaaatta ccgcttcctt ggtatgtatt agggtttgtg atcttttgtt tggtcaacag
    26041 cttgttttct ttacctgtag ttattgacca atcggcacat tttatcagca gctggtttga
    26101 gatcactgct ttagcggcaa ttggtctgcg tttagacttt gcaaaattca tgaaagaagg
    26161 tacacgcttc ttatcttatg gattagctgt cgggcttctc caaacagtga tggctttact
    26221 atttatttgg ctgatcaaag tttagaatga acatgaaaaa acagttgtga ttctcgcatg
    26281 gaacaatgtc cgaatcacaa ctgtttttaa ttttgcagat atccttctaa caacaacaat
    26341 tggcgtttga tttcaacccc atctattgcg tttcccatat atcctgtcag ttttccattt
    26401 tgcccgatca ctcgatgaca aggaataacg attggcaatg gattggttcg atttgcctgc
    26461 ccaatcgctc gcacagcttt cggactatca acagcaacgg ctacttcttt atacgttcgg
    26521 atttctccat aaggaatggt cgacaaagct ttccagacat tttgctgaaa aggcgtacct
    26581 ctctttatag ataaaggaac atcaaattct tttctttttc ccgcaaaata ttcttcaaac
    26641 tgttcttgtg tgcgcgttaa aattcgctga ttcgctgatt cgctttctga aattggctga
    26701 aaagatacag cagtcaatcc ggattcatct gcatctaacc ataaaaagcc gataggtgtg
    26761 tgtattttca ttttgtttgc tcctagtcaa tagactggaa cttcctcctt tttctctatc
    26821 caaaactact tgccaactaa taaaaaatgt tcttaaaagt ttgataaacc gaggaaacat
    26881 ggtaaactaa atacgttagt ttacaaaaag aatacctgtt cttttacgta aaatcaaatt
    26941 ttaaaggtgt tatttaaggt ttattaaatt ttgaatgtaa accatatata aacatacgat
    27001 tgaaaggata gaaacgatga aagtatcaat tgttgtccca tgttttaatg aagaaaaaac
    27061 tgttcctctt ttttttgatg aggttgaaaa attcaaaggt gcccatgagt tcgaatattt
    27121 gtttattgac gacggatcaa aagacggtac gttagcagca atcaagaagc tagcctcagc
    27181 ttatccttat gtccgctttg tctcattttc cagaaatttt gggaaagaag cggctctgta
    27241 cgctggattg caagcttcta caggagattt agtgaccgtg atggatgttg atctgcaaga
    27301 tccgcccgaa ctgcttcctg agatgatcaa ccggatcact acttctgata tcgattgtgt
    27361 cggcacccgg cgttcgactc gtgaaggcga gcctgcgata cgcagtttct ttgcgaaaaa
    27421 attctaccag ttgatcaata aaatcagcga tacagaaatg gtcgatggcg ctcgtgattt
    27481 ccgcttgatg actcgtcaaa tggtggatgc cgtcctagaa ttaacagaat acaaccgatt
    27541 ttcaaaagga ttatttagct gggtcggatt taaaacagaa tatatttctt atgaaaaccg
    27601 cgaacgtgtg gctggcgaaa catcttggtc cttctggaaa ttattcaact actcgattga
    27661 tggtatcgtg aacttctcgg atgcaccgtt aacgattgct tcatttatcg gcgctttgtc
    27721 ttgtgtcggt tctggtattg caattttgtt cattattttg cgggcattgt tgtttggtga
    27781 tcctacatca ggatggcctt ccatggtctc gatcttcctg ttcattggtg gaatccaatt
    27841 gttatgtctt ggaatcatcg ggaaatatat tgggaaaatc ttcttagaaa cgaaacagcg
    27901 tcctgtttat atcataagag aaacagaaaa agtgaagcca aatgaattga agcctcacca
    27961 ataatatacg tcaaatgaag taaacatttt tttaaatgaa aaaggactgt gacaaaagtc
    28021 ttgtcacagt ccttttttcc gaataaatgg tgttccagaa gtagcttctt cggaaataag
    28081 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    28141 tgaccacttc tatcacaacc tctttcatat cttattactt cttcattgct gcttttagtg
    28201 ctgctgctaa tgggctttct tctggttctt ctgcttgtgc atattttttc agcaagcgtc
    28261 gttcttcatg tttggtcatt ttcttcttct gctctttttt atcaagcaat ttttctgtcg
    28321 ttccatcgta tttacaacgg aaataagcac cgtttttacc ttcgatgatt tccattttcc
    28381 ggtgacattg cgggcatcga tgattagaga cttttggatc ttttcttctg cggtagttgc
    28441 aatctgaatt ggtgcagaca tagattttgc cgtcacgtgt gtttttctcg cgcagattgg
    28501 ccccacattc tgggcatttc ttttgggtaa tcgagaaatc ttgatattct tgtttgcttg
    28561 atttgatctc attcaccaag cgttttgttt ctttttcgat tccattcaag aaggttcctg
    28621 cttgtttctt tcctgcagca atagcttcaa ggtctttttc ccagctttct gtcaaatctg
    28681 gtgttactaa agagggattt accaatacta ataattgctt acctttagga gatacttgca
    28741 gtccttgagg ggtacgttcc atcagttcag atttgattag tttctcgata atctctgcac
    28801 gcgtagctgg ggttcccaga ctgtattttt ccatttgacc aagcaagctt gcttctgaca
    28861 atggtttagg cggtgcagat agttcttttt tgatggcgaa atcgggattg attctcattc
    28921 ctttttccca tttgactgtt tccgtctcag tttcagtttc gcctttccat cctgcttgga
    28981 cgacacgatt ttgccgaaaa atgaatcgtt ctttaccaaa ttcaaccgta acttttgttt
    29041 ggctgacttt atggggttga gcaaacagtc ctaaaaaacg agagacaatc atttgataga
    29101 tttttcgctc ttcattactt aatttttcat aacgtgctct gttttcagtt ggcaaaagtg
    29161 catggtgatc ggtcacttta ctgtcttgga atacttttgt ctgttggacg atggctccgt
    29221 ttttcaaata ctgcttgacc tcaggagcaa agtcgctgac tgcttgcaat cgttctttca
    29281 tcgtactttt catgtcattt gtcagatatt tgctgtctgt acgcggataa gtaacgatct
    29341 tatgggtctc atacaaactt tgtacaagac ttaatgtttt ctttgcagaa tagccatagc
    29401 gttgattggc ttctctttgg atctctgtta aatcataagg caatggcgca gattccgttt
    29461 ttaatttttc ttggacttct gtgacagttc ctttgcttgt actgatcaat tgaacaagtt
    29521 tttgtgcttc ttcttgtgtt tttaaagcaa attgattttt ttgaagcatt ttggcttgtt
    29581 cttttccagc ttgcaacgtg atcgtgaaat acgtttgtgg acggaactgc tcgattttct
    29641 tttcttggtc tctcactaat gctagtgtcg gtgtctgcac acggcctgct gataaattat
    29701 ccgcgtactt cacagttaag gctctggtca cattcaatcc taccagccag tccgcttttg
    29761 cccgggcaag agcagattca tacaatgcat catattcttt cgcaggtctt agttgcttga
    29821 atccagtttt gattgcttta tctgtttgag aagagatcca taaccgcttc actggtttat
    29881 tgaaatgcac atactctaag atccagcgag caacaagttc cccttctctt ccggcatctg
    29941 tcgcaatgac tgcttctgag acatcttttc gctgagccag ctgcttgatt gcttttaatt
    30001 gatgtcctgt tttaggaagt ggtttgattc ctagtttttt agggatcatt ggcaacgtgt
    30061 ccatttgcca gctctgccat tccttattca gatcttccgg cattttcaat cctaataaat
    30121 gaccaagggc ccaagtaacg atcaccttgg gtccttcata ataatttttg tgcttttgat
    30181 tagcaccaag caccttgctt aggtctttcg caacgcttgg tttttccgct agaattagtt
    30241 gtttcataaa attcctttct gctttctact gtgatgggct gatgattttt caacaacgca
    30301 agtcccttgt attgctgtaa atcatcgtca cactcttcac accagcgctc atctccgtca
    30361 ttccaaaagg cgctgagaaa gttacttttt gcattgatat attcataatt ttgatggatt
    30421 tcgttccatt gtttttcgta cgcttgtcgt gcttcttcga aagactcgaa tgtttcttct
    30481 tcttcaatat cttcttccca gtcttcaaaa aaccaccaag gctcattgtc cccgaacatg
    30541 gtaattactt gatacatact cgtgctcctt ctttcagctt ttctagactc atcttagtca
    30601 cttttcaaga aagttgcaag aattttctct tataaaaggg tcatttaatg aacatcttaa
    30661 aattgttaca aatgaatctg attgtttaag gcaaagtagc gataaagtta ttgatcatcc
    30721 aaaaatagct gtcttctgga tcgattgttg gttcttttgt aaagtatcct aaaaaacgta
    30781 aggaaagata accgtggatc atactacgaa gttccctgct tcgatgaact ttttcttctt
    30841 tcgttaattg aaaagcctca aatatctgca aaatgatctg attcgtttca tggattccat
    30901 ctaataaaac agcttcattg gtatgcggta cactgatcag taactcatac actgcttgat
    30961 ttgaaaaagc aaaggatcga taagtttctg catagatctg cagtgcttcc tgaccacttt
    31021 ttcccacaag agcccgtcgc agctggtcat taagttcctg cagaagtttc gctgttagcg
    31081 ctgttttgac atcacgaata tttttgaaat ggttatacag cgagggagcc ttgatatcca
    31141 agcgtttggc taaatgctga aaggttattt gctgaacatc catctcttct gctagttctc
    31201 gaaagcagtc gatgattttc tcttgagaca gatttctttt tttcatttcc atgtttcctc
    31261 tcataaaata aatccttcac tctttcagtt taccacgaaa aagaaagaga aaaaagagac
    31321 tttcattgat aaactacata ttgtagttta taataaagag ctgtagatga aaattttcag
    31381 aaagaaggag tctaattggc tgcaagaata aaggaaaaat tatttaggaa aagagaaact
    31441 tgggaacaaa acttgtttgt cttatggttt ggaacattta tggctggtat cggatttagt
    31501 ttagtcatgc cttttatgtc actttacata gatacgttag gtaattatag tacgtcacaa
    31561 ttgaactttt ggagcggatt gactttttca tctactttct tggtgactac gcttatctct
    31621 ccttggtggg gaaaattagc cgaccaaaaa ggaagaaaat tgatgctgtt gcgtgcttca
    31681 ttaggaatgg caatcgtcat ttctttgatg ggttgcgtga ctagtgtgta tcaattagtc
    31741 gcattgcgtc tgcttcaagg gattttttct ggttatatca gtaatgccac tgcccttgtc
    31801 gcaactggga cgccaaaaga aaaaagtggt caagttcttg gaaccttagc aactggttct
    31861 gttacaggaa cattacttgg accgcttctt ggcgggatct ctgcttcagc gtttggctat
    31921 cgccctacct ttttcattac agggatcatc ttatttttcg tcttcttgtt gagtctgttt
    31981 ttcgtccatg aaaaatttgt cccagtgaaa aaagaaaaca tggtttctgc caaacagatt
    32041 ttcaaagact tgaaatatcc acacgtcgtc atcggtatgt tcgtcacaac gatgatcatc
    32101 caagcatcca acaattccat tagtccaatc atcagtctgt acattcgcca attgctgcat
    32161 ggacatggga atgttacgtt gatcagcgga atcattgctt cgatacctgg gattgccact
    32221 ttgatagccg ctccaagatt tggtcgctta ggtgacaaga ttgggagtga aagaatactt
    32281 gcgattggtt tgggttttgc gattctggtt tatatcccaa tggcattcgt cacgaatgtt
    32341 tggcaattag ctgccttacg ttttttgatt ggtatctctg atgcttgtct gctccctgct
    32401 gtccaagcat tgatcacaaa atattcgcct catcaagctg ctggacggat cttcagttat
    32461 aaccaatctt tccaagcaac tggtaacgta gtagggccta tgatcggctc cagtgtttcc
    32521 agtgcatttg gttatcgtgg cgtctttctt tcgacttctc ttcttgtttt ggtcaacttc
    32581 ttgcttgtac gacacaatac acaagagatc catcagcaag aaactgcaga agaaaaagct
    32641 tcaggagtct ccatagcttc agggcactct ccttctcata gacaataagc gtaaatgaaa
    32701 aacactgctt gaacagttgt cacagactgt ccttgcagtg tttttgttta ggcctgtttt
    32761 ttcaagaact cagataagag gaataatccc ttttccaagg tttctttttt acaacagtaa
    32821 cctagtcggg catgcttcgg taaatcaaag gcacttcctg gtactaatag tactcctgtt
    32881 tcttctaata aattgataca aaatgtctgg tcatcttctt cgatggctaa tttaatgaaa
    32941 gaagtagaga cgtaattggg agcgacaagt tcaactttcg gttcattggc tacccattgt
    33001 tttaaaatca caaggttctc tgtaatgatc tttttgtttc gcgccaatat tttttcttta
    33061 ttcttcaatg catgtacagc aagatcatct gacaggactc cgccgcaaat catcgtgtag
    33121 tctcgatact ttcgaaaaac ttctgccaac tcaggtccag tagctgtcca gccaatacga
    33181 atacctggaa tggagtaggt tttggatagg gaattggttg ctataccttt ttcatagaca
    33241 tctacgatcg acaagaattc acctttatcc gttaaaggcg catatacttc atctatcaat
    33301 acatatgcat ctactgtacg agcaatttct gcgatttttt ctaaagtttt ccgatctagc
    33361 aatgtgcctg taggattgtt tgcactattc aaacagatga tttttgtttc aggtttgact
    33421 aaggcttcta gctgttctaa atcaaactgc caatcttctt cttccttcaa atgaacaaag
    33481 tcaacagcgg cacctaaaga tttggggatg tcatataatt gctgataagt aggaagcatc
    33541 gaaacaacat ggtctcctgc ttccactaac gcgtatagag ctaaaagatt agcaccagtc
    33601 gctccattcg tttgtaagat gttttccgga tcgacggttt gataaagaga agcgacttct
    33661 tgtttgaaaa catctgatcc ttcgatccag ccatagtcca atggttcgtt tttgcgtttt
    33721 tcgaaaaatt tacttacgct agtcccatct aagccaacga gttcgtctaa tgtcaatgct
    33781 tcgatcgagc tttgtgcaat atcatatacc gctttctttt cccatttatt tagccattct
    33841 tctacaccga aatttgcgat tttcatgttt ctttccccct tatatcttga tccagcctac
    33901 agcaagcaac ccgattgaaa gcagtgcggc cacacaaatc agcccaatca ttactttttc
    33961 ttttcccgta atttgatgtt tggtttcacg acaagcgatc cagtagaagc aaaaaccagg
    34021 aagataggca atactacata aaaggacata agaaaatcct gctaaaatga tgcccatcaa
    34081 ttcaaaaact acagcaatcc caccgatcac tacttgtttt ttgattcctt cttttaaaga
    34141 aaactggact tgatagatcc cgacaagtag ataacaaatc aaaattgccg ctgtacataa
    34201 agaataggca aattgatagg cataatcagt aactaagaaa gtcaataaga atagattagt
    34261 caagattgca gtaacaacta acgaataagt tggggcattt tttttattcg tttttcccca
    34321 acctttcggc aacagaccgt ctcgagccat caacatcgtt gtttctgctg ggagcatcgt
    34381 ccaagacaac caagaaccaa tgatcgaaat aatcaagccg atattgatca gcgctgctcc
    34441 ccaatttccc acgattcctt ttaaaatatt tgccatagca ggttgtgaaa tagttgctaa
    34501 ctcttcttga gataagacgc cgtatggcaa gatcgaagca aggatataga taaacaataa
    34561 acaaagcaga ccaatgatcg atgcttgctg ggcttctgaa cgtttcttcg cgcggttggc
    34621 taaaacactt gcgccttcga tccctacaaa gacccacatc ataaccatca aacaaccttt
    34681 aatctgcaac cagatcgttc cttgctgccc tgttccattc actagattat cagatacgtt
    34741 accccaaaaa tcagcagtga atatattcac tttaaatgcg ataaatacaa atattaaaaa
    34801 taggaataat ggcactaatt tacagatcgt cacgattgta ttgatgacgc tcgctccttc
    34861 aactccattg tttactaata aagtcagacc ccaaataaaa atacttgcaa ataaaataga
    34921 aggaacattt tgtccacctt tgaatatcgg gaaaaattct cccatggcgc tcatcagcat
    34981 cgttgcaaaa gcaacatttc ccagccacgc agacaaccag taaccccaac cactgatgaa
    35041 gccacctaat ttaccaaatg aagcttccgc gtaaccgaaa ataccaccat ctagatctgg
    35101 acgcttttct cctaaatgat tcagcgataa taccaaagct aaaataccaa tcccaacgat
    35161 tatccatgaa aggatcgctg gccctggagc tgcagaacta gccagatcac ttgtgatccc
    35221 aaacacaccg cttccgattg acgaactgac aaccagagca accagtcccc atttgctaat
    35281 acccgtcgat ttcttttcca tttccatcac cctcacttat aaaattgaac aaatcacttg
    35341 caaaagattc ccttctattt tgcaactgct cttgcttaac aaaaagcaac tttattcata
    35401 ttattggata ttatgaactt tttacacaag tatttcaagt ataaaatgaa taaaaattaa
    35461 ttttattgca aaaaaacagc tatgctatcc aaaagaaaag gtgtttcttt tgttagccat
    35521 agctgttttg attcatttaa tatcgcttaa acgtattgta aaacttttgt ttcttgatat
    35581 gcgtggtcga tcaaaccacc acccagacat tccattccat cataaaatac aactgcttgt
    35641 ccaggagtga ttgcacgtac aggttcgtcg aatacgactt ctgcacgatt atcatccaat
    35701 aagcgaactg tcacaggaac gtcttgctga cgataacgga atttcgctgt acacttgaat
    35761 tcttttggca ttggttcatt cgtcgtaaag tggatttcac ttgcatccaa gcttgtggca
    35821 tacaatgcag gatgatggaa cccttgcccc acatataatg tgtttgtcgc tagatctttt
    35881 cctacaacaa accagggttc ttgcgaatct cctccgccac cgatccctag tccttgacgt
    35941 tgaccaatcg tataatacat caagccagca tgttgtcctt tgacttcacc atcaagagtg
    36001 accatatttc ctttttttgc tggcagatag ttgctcaaaa attgtttgaa gttcttttca
    36061 ccaataaagc agattcccgt tgaatctttt ttcttagcag tcgcaaggcc tgcgcgttca
    36121 gcaattgccc gtacttctga tttttccatg ccgcctagag ggaacatcgt ttttgaaagc
    36181 tgttcttgtg ataattggct aaggaagtaa gtttgatctt tattattgtc tactccacga
    36241 agcatgtgga ccacgccgtt ttcatcgcga gtgacttgcg cataatgtcc agtagctaca
    36301 taatcggcgc caagatccat tgcataatcc aagaatgctt tgaatttgat ttctttgttg
    36361 cacatcacgt ctgggttagg tgtacgtcct gcgcggtatt cggctaggaa atattcaaat
    36421 acacgatccc aatattcttt ttcaaaattc acagagtaat aaggaatccc gatttgagca
    36481 gctactttcg ctacatcctt ataatcttcc gtggccgtgc atacaccgtt ttcgtccgtg
    36541 tcatcccaat ttttcatgaa tattccgatc acgtcgtatc cttgttcctt taacaaaagt
    36601 gctgttacag atgagtcgac accgccgctc atccctacga caacgcgtgt tttgctgttg
    36661 tctgtcatga tatcaccatc atttcaatta gattctgaaa catctacgag ttgaaggtcg
    36721 caacttttca gtattttaca ttatacgtca tatttctgaa aatttcatcc cttaattatc
    36781 aagtcgtaaa aatactgttc tactggacct attcagtaca caaacatgat agtcaaccaa
    36841 aaaatcactc atcttttgag cattcgatat aaaaaattgg catactcata aaaagggcca
    36901 gcctttcggc aataaggaat aattttataa attgtgaaaa actatttttc aaaattgctt
    36961 cttatcgctt aaagctgcac gtctcattta caaccttttc tttttagatg ttatgagttg
    37021 ctcagataag tgattttgtt tactctaaga aaaaagggag gcaaaagata catgaattac
    37081 tttatcagtg atatgcactt tttccatgaa cgcttattag gaaaaaacga ctttgctccg
    37141 cggctctttg caaatgtgga tgaaatgaac caacagttga tcactagctg gaatgcagta
    37201 ataaaagaaa ccgaccatgt ttatcatcta ggcgaccttg ctatgcatcc gcaatatgaa
    37261 aaagcaaatg cagaaatttt ggaactggtc actcaactgc aaggaacaat ccacttgatc
    37321 aaagggaacc atgacagtcg cgcttttttc cgttatttag aaaaaaatga tcctaggctt
    37381 actgacggaa gtcccaaatt ttatttttat gatgtaggtg ccatcgttaa attcgaccat
    37441 caacaatatt acttgactca ttatccgatg ttgctcgggc ctaacgacac tatccgcaat
    37501 cttcatggac atatccatca ttacagcgta cctatcgaga acgatgtcaa tgtaggcgta
    37561 gacgcaccgg aaagagaact cttaaaggaa aaactaccat ttggcactcc gttatctgag
    37621 caacaaatca aagaaatcta tgaagcaaag aggaagtacg ataggtcgtg aaaaaactgc
    37681 cctatatcaa gtattaaaca aataaagata attactcata tttttcattt gagtaggctt
    37741 attgccacag gaacattagg atcagaaatg gagttgtaac aagacttctg tcacaactcc
    37801 attccatact attcgtcttt ttgctcatgg tgttctttta ccagtttttt ctgtaattct
    37861 ttatcataat ttcgggcagc tgacgttaaa aataaaacga ttccgcataa taaaatcccg
    37921 attccggaaa acaagaacat tttttctacc cctaaacgat cagctaatgg tcctgcaaat
    37981 aataatccaa ttggacctgt caggttcatc aaagagttga agttccctaa gacacgacct
    38041 aaaacattcg attcataact ctgctggatc attgccatca gcagtgtatt gaaatatggc
    38101 gttgcacaac ctgctcctgc attcaagata agaaagtaca agaacccttg actatcgttt
    38161 ggaaccaatc cgcttgcacc gatcgtcagg cccataatga tgtaggctat aataataggt
    38221 ttcattcggt ctttccatgt tcctataaaa ctgatcagtg caccgcctaa gagcattccg
    38281 actgcgtaga ctacttccac tgctcccgcc tgtccaacgg aaccaccaaa gtaatctagc
    38341 gtcatcaatg gatacagact gattgctggc atgaacaaca gcatcacaaa tgcaccattg
    38401 atcgttatgt accataaccc tttattatcc atcaattgct gcagcccaaa ttttgcatct
    38461 ttaaaaatag taagcggaac ttccaatatt tcaggagaaa cttttgggat cttcacaaaa
    38521 atcaataagc cgacaccaaa aactgccccc aatacatcta ataaaataag gtaattgaca
    38581 ggaacgactg aaaacaaagc cgcacctaga gctggcgcaa tgatgaaatt cgctgattgg
    38641 accattccta actgtccatt cgtccttgtt atgtgagaag atggaacgat tgtcggcata
    38701 atcgactgga tcgttggcat ttggaatgtt tgtgcgaccg aacgcataaa caaagacaca
    38761 aaaactagcc aaatcgggaa ggattctgaa atcgttccga ctactgctaa aataagcgcg
    38821 aaaatcgcta caataatatc cgtgacaatc aaaagagctt ttttatccca gcgatccact
    38881 agcggtccta caaatgggct tagtaaaacc atgggaatca ttcctagtaa agtagcaaaa
    38941 ctcaaaattg tcgccgaacc ggtttctctt gtcaaatacc agatgatcgc atattggacg
    39001 accatactag tgattcctga caaaaactgt cccgataaaa agaacaaata atttctcttc
    39061 caattcgtta aaacgaccga ctgttcattt tccataaaca tccctctttt ctttttcatt
    39121 ttgtatagcg tttgcacaac aatcattata cgggattctt ttttcaaaaa ataccttttt
    39181 ccctaaataa caatcgtttc aatttaaatt tctttactta gaaacaataa atcagcaaaa
    39241 tattaaaaat aacaacatgt gttaaattta aaaataaaaa agacaactaa tttgcgtcgt
    39301 ccttacaaag ttagtcatct aacaataggc cgtttcaaaa ctagttgtct ttttatatcc
    39361 acagatcatt cgactttttc gaataaccca cggctaatca tcccatccat cagaaaataa
    39421 aatttatctt ggctcatttt ctgttttgga tctttgaaaa tattgactgc tttcaatcca
    39481 tttgctgtag tgatcgacat tttcaatgtt tgaatatctt ttgatacagt gatttttaat
    39541 ttgaatcctt ctttgctttg cggcgtggca tctaatggtc ggatcaattg ataattgcct
    39601 ccgtttgttt ctgtaaatcc attatcgcgc aatgtatatc tttttgcatc aggatgcaaa
    39661 cgatagctaa ttggtgcgcc cagtaccgtt gctgttgttt caaatgccat gattttcacc
    39721 tcgataaaat tagtttctct ttattataaa agtttatcct gatttttact aggtttttca
    39781 gttaagttcc ctaaaaaatg aaggcgttct ctcagacatc tgttcatata agaatgactt
    39841 cactagttat tttttgacag cacgaactaa ttgatcaagg aaatactgga tgtcgtcttc
    39901 ggtatttccc aaaccaaagc tgatacggat ggattctttt agtatcggtg tatcttctcc
    39961 atacattgct tccaatacgt gtgaaggctc gatatttcct gctgtacaag ctgaaccagt
    40021 agaaatagcg accccattaa gatcaagctt catcagtagc aaatcatttg cttcgccaat
    40081 taagcgaaga ttcaacacat gagggagttt attcgttaaa tcaccattta cttgataagg
    40141 gatttcgttt gcttctaatg tatcaagaat caattgctgg aatcctttat atttgttgtt
    40201 acgtgcttca cgttcttcag gtgacagcag ttcaactgct tttgccatac cccagatccc
    40261 tgctaaattt tctgtacctg cacgccgctt ttcttcctgc tccccgccta aaagcaatgc
    40321 agggatattt actccgtctc gtttatataa gaaaccgacg ccttttggcc cattgatttt
    40381 atgagcagag atacttaata aatcgattcc taattcttct ggatggatcg tttggtttcc
    40441 gtacgcttgt accgcatctg tatggaaata agctggatga tccgctaaaa gttcaccgat
    40501 ttctttgatt ggcatcaaat ttccgatttc attgttcccg tacatcacag agaccaagat
    40561 cgtatcttct cgtaacgctt ttttgacctc ttcgatcgac agattccctt tttcatctac
    40621 aggcagatat gtaacgtcaa acccctttgt ctctaaatat ttcatggtat taagaacagc
    40681 aggatgttcg atagctgtag tgattagatg tctgccttct ttttgacgcg agaacgctgt
    40741 ttctaagata gctgtattat ccccttctgt tcccccactg ttaaagatga tctcgtgagg
    40801 ttttgcttgc agactgtctg caatgatttg tcttgctgtt tccaattttt catgcgcgtg
    40861 gcgaccaaat ccatgtatgc ttgaaggatt tccaaacgtg tcttgcatga ttgccgtcat
    40921 cgcttcaatt acttgtggat gcatgggggt agttgccgca tgatccagat aaatagtttt
    40981 cacttcattc atcccttcca aattattgct aggcacattc tagcataaaa tagcgaacaa
    41041 accaaagaag agctcatttt tgtctagagg acgtttcatg ggcatagaaa aaaggagctg
    41101 tgacaaaagt cttgtcgcag ctccttgaag ctgctcactt ctgtcacaac ttctt
//
LOCUS       DACDTH010000019        40074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDTH010000019 DACDTH010000000
VERSION     DACDTH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.019"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..290)
                     /locus_tag="IWK54_001061"
     CDS             complement(<1..290)
                     /locus_tag="IWK54_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9593201.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
     gene            complement(283..624)
                     /locus_tag="IWK54_001062"
     CDS             complement(283..624)
                     /locus_tag="IWK54_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593202.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(798..1523)
                     /locus_tag="IWK54_001063"
     CDS             complement(798..1523)
                     /locus_tag="IWK54_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9593203.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1576..2052
                     /locus_tag="IWK54_001064"
     CDS             1576..2052
                     /locus_tag="IWK54_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593204.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(2058..2240)
                     /locus_tag="IWK54_001065"
     CDS             complement(2058..2240)
                     /locus_tag="IWK54_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593205.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(2240..2539)
                     /locus_tag="IWK54_001066"
     CDS             complement(2240..2539)
                     /locus_tag="IWK54_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593206.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2644..2847)
                     /locus_tag="IWK54_001067"
     CDS             complement(2644..2847)
                     /locus_tag="IWK54_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9593207.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(2862..3608)
                     /locus_tag="IWK54_001068"
     CDS             complement(2862..3608)
                     /locus_tag="IWK54_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9593208.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            3686..3994
                     /locus_tag="IWK54_001069"
     CDS             3686..3994
                     /locus_tag="IWK54_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593209.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(3965..4222)
                     /locus_tag="IWK54_001070"
     CDS             complement(3965..4222)
                     /locus_tag="IWK54_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAP9593210.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            4960..5280
                     /locus_tag="IWK54_001071"
     CDS             4960..5280
                     /locus_tag="IWK54_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9593211.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            5298..5729
                     /locus_tag="IWK54_001072"
     CDS             5298..5729
                     /locus_tag="IWK54_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9593212.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            5856..6440
                     /locus_tag="IWK54_001073"
     CDS             5856..6440
                     /locus_tag="IWK54_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP9593213.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            6542..7690
                     /locus_tag="IWK54_001074"
     CDS             6542..7690
                     /locus_tag="IWK54_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9593214.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(7779..9404)
                     /gene="groL"
                     /locus_tag="IWK54_001075"
     CDS             complement(7779..9404)
                     /gene="groL"
                     /locus_tag="IWK54_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9593215.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(9455..9739)
                     /locus_tag="IWK54_001076"
     CDS             complement(9455..9739)
                     /locus_tag="IWK54_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9593216.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            9964..10626
                     /locus_tag="IWK54_001077"
     CDS             9964..10626
                     /locus_tag="IWK54_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9593217.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(10702..11994)
                     /locus_tag="IWK54_001078"
     CDS             complement(10702..11994)
                     /locus_tag="IWK54_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9593218.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            12164..12793
                     /locus_tag="IWK54_001079"
     CDS             12164..12793
                     /locus_tag="IWK54_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP9593219.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(12896..13705)
                     /locus_tag="IWK54_001080"
     CDS             complement(12896..13705)
                     /locus_tag="IWK54_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9593220.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13760..14629)
                     /locus_tag="IWK54_001081"
     CDS             complement(13760..14629)
                     /locus_tag="IWK54_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593221.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(14630..15946)
                     /locus_tag="IWK54_001082"
     CDS             complement(14630..15946)
                     /locus_tag="IWK54_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593222.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(15943..17778)
                     /gene="walK"
                     /locus_tag="IWK54_001083"
     CDS             complement(15943..17778)
                     /gene="walK"
                     /locus_tag="IWK54_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9593223.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(17783..18487)
                     /locus_tag="IWK54_001084"
     CDS             complement(17783..18487)
                     /locus_tag="IWK54_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9593224.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(18677..19537)
                     /locus_tag="IWK54_001085"
     CDS             complement(18677..19537)
                     /locus_tag="IWK54_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9593225.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(19524..20093)
                     /locus_tag="IWK54_001086"
     CDS             complement(19524..20093)
                     /locus_tag="IWK54_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9593226.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            complement(20667..21836)
                     /locus_tag="IWK54_001087"
     CDS             complement(20667..21836)
                     /locus_tag="IWK54_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9593227.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            complement(21880..22110)
                     /locus_tag="IWK54_001088"
     CDS             complement(21880..22110)
                     /locus_tag="IWK54_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9593228.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            22186..22258
                     /locus_tag="IWK54_001089"
     tRNA            22186..22258
                     /locus_tag="IWK54_001089"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22219..22221,aa:Lys,seq:ctt)
     gene            22420..22641
                     /locus_tag="IWK54_001090"
     CDS             22420..22641
                     /locus_tag="IWK54_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593229.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(22965..23210)
                     /locus_tag="IWK54_001091"
     CDS             complement(22965..23210)
                     /locus_tag="IWK54_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9593230.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(23204..23605)
                     /locus_tag="IWK54_001092"
     CDS             complement(23204..23605)
                     /locus_tag="IWK54_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9593231.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(23777..25585)
                     /gene="uidA"
                     /locus_tag="IWK54_001093"
     CDS             complement(23777..25585)
                     /gene="uidA"
                     /locus_tag="IWK54_001093"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP9593232.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(25613..27379)
                     /locus_tag="IWK54_001094"
     CDS             complement(25613..27379)
                     /locus_tag="IWK54_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9593233.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(27392..28186)
                     /locus_tag="IWK54_001095"
     CDS             complement(27392..28186)
                     /locus_tag="IWK54_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9593234.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(28197..29573)
                     /locus_tag="IWK54_001096"
     CDS             complement(28197..29573)
                     /locus_tag="IWK54_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9593235.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(29702..30349)
                     /locus_tag="IWK54_001097"
     CDS             complement(29702..30349)
                     /locus_tag="IWK54_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9593236.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(30433..31860)
                     /gene="uxaC"
                     /locus_tag="IWK54_001098"
     CDS             complement(30433..31860)
                     /gene="uxaC"
                     /locus_tag="IWK54_001098"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9593237.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(31934..33013)
                     /gene="uxuA"
                     /locus_tag="IWK54_001099"
     CDS             complement(31934..33013)
                     /gene="uxuA"
                     /locus_tag="IWK54_001099"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9593238.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(33016..34647)
                     /locus_tag="IWK54_001100"
     CDS             complement(33016..34647)
                     /locus_tag="IWK54_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9593239.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            34964..35635
                     /locus_tag="IWK54_001101"
     CDS             34964..35635
                     /locus_tag="IWK54_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9593240.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(36471..37427)
                     /locus_tag="IWK54_001102"
     CDS             complement(36471..37427)
                     /locus_tag="IWK54_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593241.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(37536..37781)
                     /locus_tag="IWK54_001103"
                     /pseudo
     CDS             complement(37536..37781)
                     /locus_tag="IWK54_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(37926..38348)
                     /locus_tag="IWK54_001104"
     CDS             complement(37926..38348)
                     /locus_tag="IWK54_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593242.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(38487..38917)
                     /locus_tag="IWK54_001105"
                     /pseudo
     CDS             complement(38487..38917)
                     /locus_tag="IWK54_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(39233..39496)
                     /locus_tag="IWK54_001106"
     CDS             complement(39233..39496)
                     /locus_tag="IWK54_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593243.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT        12183 a         7960 c         6117 g        13814 t
ORIGIN      
        1 tctgtttccg tatgtccttt aaccgttact gagacagata cataagtccc tgtttcatcc
       61 ataagaaaag gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg
      121 tagtgtttat actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc
      181 cacgcgtgag cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac
      241 ttacgactaa aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc
      301 ctccatttca gcaatgtatt tcttacctga tccgtaataa gagatatcaa tcaagttatc
      361 tctgttgtac tcttctagcg catcaatcaa gccatcttcg atgacataga tgtattcagg
      421 tttatttgaa tgttttgata gatggataag gtaaacatga tcccaaatac tcacaaaatt
      481 tcccaagtca tcttgatcac atgatagttc ttcgttcgtc aaaagatttc gtctgatttt
      541 tcgattattt gtttccttga tattcgactt gccccattca gaatcagtca aatattgatc
      601 taaagtggaa agttctttat tcatatgcta aaattctcct tagatatgtt ttgtgactct
      661 atgcttgccg gctgagtcac ttttttattt gttgccatgc tttttgcttt tcgatatgtt
      721 gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt accgtcccta
      781 ttcttagcgc ttcagctcta ttcattttca tcaccgaaaa gtctttgttg tctgttcagt
      841 tgatcgattt ccatgcggat cgcagtttct ggtaaccaca tttcaataaa tgaaacagca
      901 tcatcgaatc tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat
      961 tcagaccaaa attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt
     1021 ccccctagaa cttccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt
     1081 ccgctaattc gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct
     1141 tctaattgat agatcatcat gtcttcaatt gtttgaggaa cagtattctt ccgaataaca
     1201 tcttccattt cgttgaaagc ttcaataaat tttagtttga aatttattgc tttacttcca
     1261 gtgaatccca tagctagcaa ggaaaatccg tctctattca tgaaataaac tctccgactt
     1321 cttccgtatg agtctggctc gttcccttcc acaaacatct gtccaaaatt ggacccatct
     1381 tcaacattgg ccgaattttc gaccgatctt tttattgatt caattgcttc tagtacatgc
     1441 ttatgtttct ttttgaaact ttctgccact tgtaagctcg tagttacagc ttctttattt
     1501 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat tttcgttaga
     1561 aagtgaggtg attctatgag gaaaaagctc aattacattc ttactgcgac tttaaccata
     1621 tatataattc tgtttattac cttatctttc ctgtcttcaa ttaaaaaaat tccttttatt
     1681 acattgaata aaaatagtat tttgaaggag ttcaataaca ctgactacat cgcatttcta
     1741 aaactttttt cagtctgtgt gctaatatca tgtgtaatta tttttattgt tgctaaaaca
     1801 tgttggacaa tgttaaataa aaaccaacgc gatgttgaaa atattgctat atttttaatt
     1861 aagattactt cctcaatatc aattatcatt ccgatgactg taactttttc aaaagaacag
     1921 tttaatgtag cggctactta tctttcattt atagcgctat tttcttttgt tcttcctaaa
     1981 agaaataaga gcaactttga caacaaggag ctggatgaag tacgcaatag cagcccaaag
     2041 gaacaaaaat aaaaaaatca tattttagtc agtccctccc gactggcttt tttcgctctg
     2101 tgctcagctt catcaagccc cataaaaatc caaaccatgt aaacgattgt ccctatcaac
     2161 gcttgtctgc ttccccagag gcctaaagcg tagatgatta gcggtgcgct gaatactagt
     2221 gctctgttga atttgcccat taacttgcct ccttcaaaac atctgaaggc gataaacttt
     2281 ttacacgata tttgtttgcg tctttccact ttaaaaacca aataaaagta tggtaatgaa
     2341 taaatgtggt tgagtgacct ggacgaagta ttccttctgc aaattcagga attgtttcca
     2401 tttccttgca gtattcaact aaagtagttt ttgacatgcc gtggaacttc tccaagatta
     2461 aactttgtcg gtaccagtcg tctggtttta ctgccttctc agcagcttct atcagctctg
     2521 aaagtgttgg ttttttcact tattatccct cctaaaaatt atcgttccat actcgatgtt
     2581 taacatttct tgaagctctg attttgtaag aatgatttta tcttccaacg actttacctc
     2641 cctctacaat tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt
     2701 ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag
     2761 catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg
     2821 ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa
     2881 tccatttgag ggtaatagcc ctctgcgatt aataagttgt aaataaacac tcgccctttc
     2941 tgtgtccatt tggtattcat tacaacttta gtgccgccat cggctttcgg tatctcactt
     3001 gtgtgcgatt ttgtgtatcc ttggttcata tgttttcggt aaagtatcca ttgtccgcta
     3061 actttatgct gaacacctaa ctcgtttagc aatttgttta gagcaattgc cgacattccg
     3121 taatcagctg caatttgaga agtagctacc gtatctgtcg atgaaagaat cgtatctaag
     3181 tagctaatct ttggttcgta ctcggcaatt tgttgttcga gtaattggtt cttttcttct
     3241 aaatccgctg ctaaacgcaa tgcttgtgca aaactttgag gtacgtttga atagctacct
     3301 gtttttctaa ttgttggaag gacttcactc gttacccaac gtttaaattt tttggcagag
     3361 ggaagttttg attttaagat taaactgtaa aggcctgact cgttaatcaa aacaactgct
     3421 gttccattga cggtgaacga atcgttctgc gtcttatcct ccaagtccac atgatctcga
     3481 atagcttttt gcggattcga atatcctaga acgtctgcta catcttttcc tacaaaatat
     3541 ggttcatcat ttactaaaat agttcgaact tcgttttgct cgaaattaaa aatttgtggt
     3601 gtattcatat ttctcattcc tttctttggt ataatttcct tatcagtgtg acaggctgaa
     3661 ataattgtta aggaggtgaa aatttatgaa accagaattt gtaattacta tgcttgatgg
     3721 agaaaaaatc aaaatatttg ccgatacttt ggtcgttgga tacgatgatg cgtcaaatat
     3781 cgaagaaaaa gatggaatgt tctattcttc tcaaatttac gtgaatacct tgcaaggtga
     3841 tattggggta tctactcttg caacatcaag accgcttgta ggtcttatcg gctttttgtc
     3901 aaaagcaaca ttcttttctc tcggcgatga tatagattcg gatgaaactg tcatctataa
     3961 aacatcagct gttaaaagta tcggtttcaa atagtaattc ttgctcgttg atagcggaag
     4021 ccaacttttc ttccaacaca tcggcgagct ttctttttgt tggattcatt tcaaagttga
     4081 tagtcttgag aagcattttt gcatccttaa ctttttgtcc ttgcaattcc ttgatgattt
     4141 tatcggcttg gctatcaatt ccatgtgtta gatcatctat cattttttgt accttgattg
     4201 tttggctcat ttccttgttc attgtttagc ctcctttaat aaatgatttt ttgtattttt
     4261 taatttccat aaattcctca aattgctctt tggtattatt accacgacca tacatattgt
     4321 gaaattcttt atggcaactg acacaaagtg taacgccgtt tgaaatcgct gcggatttag
     4381 ttgttgcatg gctaaatcac gaatcacaag tatctactaa aggcgaatca atttcgccaa
     4441 aagaaattgt tcaagcttat ttagatattc attatgctgt tatctatggt cagcttccag
     4501 aagaccgtaa aaacgatgac taaacgtgag ctaaaatctc tgctatggct gcaaccatgg
     4561 cagagtcttc tttatctacc gcgtgttcca tcgccacttt tgcttgtttc agtatgtcta
     4621 atttcaaatc ttcgattttt gccgagactg ttttttccat attcttctct cctttctttt
     4681 atattcgtaa acaaatttaa caacttttta aaaaactgcg ttgactttat tagattaata
     4741 atctatacta aatgcatagt taaataagac atataaactt tgatttaaaa agctttcttg
     4801 gcggttggca tttattaatc aatagtattt tttgttgtct ttttagttgt taaacttgtt
     4861 tacaaaacat agtatagatt aataatgtat ataagtcaac tatttttaca ttatttatct
     4921 aaactttttt tgtaagcatt cagaaaggtt gatttatcaa tgaatactta cgaaataata
     4981 aaggagttag caaaagaaaa aggattatcc attagacagc tcgaaatgaa tttcgggtat
     5041 tcgaatggat atttaggaag ttggaaaaga caaactccta attctattga gttagctcgg
     5101 ttagctgatt actttggagt ctctgtagac taccttctag gtagagaaga aagagaaacc
     5161 cctaaacatg tggatttatc agaaaacgat actgtttttt cttttgatgg aaaagaaata
     5221 tctaaggaga caatgcgtaa agcgattgca attgctaaag ctttagagga aaatgaatag
     5281 ttggagtgat gggttgtatg tatttgaaat tgaaagaaat gttgagcgaa tacaatttga
     5341 aagtagttta catggaaatg aaagagcctg gcttctatta tccgaagcct agaatcattt
     5401 ttttgaatga aaatctatat ggcgaaactg cagaagcctt tcatttatct catgaacttg
     5461 cgcatttcac cgcttctcat tttgaatatt cagttttgta cgatacttct acaacttttc
     5521 actcaaaatt tgaaacagaa gcagataaaa tagctattct aattttatta aatatctata
     5581 ttgagaacga actgactgat gaatctcagt ttaatctcga aaaatttatg gagtattatt
     5641 ctattcagaa taagctcaga tacacttgtt atgctgtctg ccaatgctat tttaagaaaa
     5701 aatattctta cgcaaggcaa tatgtatgaa tacatcaaga attattaaaa aaaccgattt
     5761 cagcaaaaca ttaaaaaagc ccgtgctggc acacggactc atacctcatt tctgagatcg
     5821 caaatatatt ataacaagaa gtgaggaata tttaaatggc aaaaaaagtt atgggtcaag
     5881 acggaaaaat gtataaagtt agtaaacctt tttacaaaaa agtatggttt tgggtattag
     5941 cagtaatttt aattataatt attggttctg ctttaaatgg tggatcagat agtaataaag
     6001 caagtgataa tggtggcgaa aaagtaacta aatcttcaac ctctgcttca tcttctaaag
     6061 aagaaaaaag cgataccttc tataagattg gtgacactgt aaaagttggt gatgctgaat
     6121 atacactaaa tagtgtagaa ctaactgatg aaagaaatca atttgaagaa aaccaacctg
     6181 cacaagtagt aaaaattact tataccgtaa aaaatgatgg cgattcagat atccctgtag
     6241 gtacagatgt ggaagtatat ggaccagatg acaaaaaatc agagacatat gccaacgaaa
     6301 atacaatggg atctgttgct ccaggaaaac aaatggatgt aactgctcat ttcacgttaa
     6361 accaaaaggg agaaatagaa atccacttct ctcctttagt atcatttgaa aaagcagcta
     6421 tttttaaagc aactgtataa taaataaaaa acacgcccca ccgtccagag taagagcgtg
     6481 cttttagaaa acaaacctat ataggtctat ttgttatgcc tattatagca aatgatagga
     6541 gatgttaaaa gtgtgggttg aacagaccaa agatggccaa ttcaagttta ttgaaagata
     6601 tgtagatcct tatacagaaa aaacacgaaa aaaatctaca acacttacaa gtaattcacc
     6661 acaagcgtgg aaaaaagctc agaaaattct agataaaaaa attaaagaag cactcgaaga
     6721 ttacaataaa tcagatatca cttttggtga gctgtataaa gaatggtatg aatattataa
     6781 gcagcatgtt aaacgtacta gctatttgaa ggttccaatg atgatgaaac atgtttctaa
     6841 gcacataagc gatgatacga tcgttagaaa cattgatgag acactcatta ataagataat
     6901 tgaagatatg tatacgtttg gtgacctctc actgaactac acaaaacaaa caaaaacaac
     6961 tctatctgtt atgctaaact atgcaatcga tagaaaatac attcaaagaa accctgcgct
     7021 agcagttaaa atccatccta aaaaagtgga agaagaaaaa agaaggcttt ctatgaataa
     7081 aaaatatctg gagaaagaag aaattgatca aatactgaaa cagctatact ccaatcctcg
     7141 cagaaaacta cacggcataa tagctgagtt tctatattta actggtttga gatatgggga
     7201 attgctagct ttgcaaatga aggactatga ggatgggaaa atttccatta atgggacctt
     7261 agattacaca tctgtgaaaa tggataatgc tataaaaaca actccaaaaa atacttattc
     7321 gcaacgtgaa gtgcaattac ccaatcgtgc aaaagaattg attgaaagtg tgatagctga
     7381 caatattctt gcaggtagac ccacagatcc cgatcaatat atatttatat ctacaagtgg
     7441 cactccgctt gcgctgcact catttaacgc tatactccat aaagtagagg aagagttgga
     7501 attagaaaaa agtctatcct cacatatatt cagacatagc cacgtttcac tattatctga
     7561 attaggcgta cctcttaaag ccattatgga gcgtgtaggg cattctgatg caaacacaac
     7621 tctgtctatt tataaccatg taacaaaaag agccaaacaa caagtaattg ataaactaaa
     7681 tagcctttga tatttttgcc ccttctttgc cccttttgct taaaataaaa caaaaaaaga
     7741 agaaagaacc ctgttaaaat agggttcttt cttaattact acatcatacc gcccatcatt
     7801 gatggatcca ttgcaggagc tgctggcgct gctggttctg gtttatctgc aacgactgct
     7861 tctgtcgtta ataacaaggc agaaacggaa gcggcatttt gcaaggcaga acgtgttact
     7921 ttagttgggt ctacgatacc tgcttcaacc atgtttaccc attctccagt agcagcattg
     7981 aaaccagtgc ctagttccac gttcttcaat ttatcaacaa tcactgatcc ttcataacca
     8041 gcgttttcag cgatttgacg aattggttct tctaaagcac gaacaacgat cttgatacct
     8101 gttgccacgt ctccttctgc ttctacagca gaaactttgc tgataacgtt cactagagct
     8161 gtaccaccac ctgagaccat tccttcttct actgcggcac gtgtagcatt caatgcatct
     8221 tcaatgcgta attttaattc tttcaattct gtttcagtag ctgcaccaac tttaacaaca
     8281 gctacaccac ctgctaattt agctaagcgt tcttgtaatt tttcgcggtc aaaatcagaa
     8341 gttgtttcag caatttggtt tttgatcaat tgcacacgag cttcgattgc ttctttttct
     8401 ccagaacctt ctacgatcgt tgtattgtct ttatctacaa caactttaga agcatttcct
     8461 aagttttcga ttgttgcatc tttcaattct aaacctagat cgtcagtaat tactgtacca
     8521 cctgtcaaaa tagcgatatc ttcaagcatt gctttacgac gatcgccgaa accaggagct
     8581 tttacagcta ctacgttgaa tgttccacgg attttgttca atacaagagt tggcaatgct
     8641 tcaccatcta catcgtcagc aataatcaac aatggacgtg attgttgcaa gatttgttct
     8701 aacaatggca gaatatcttg gatgttagag atttttttgt ctgtgatcaa gatatatggg
     8761 ttttctagaa cagcttccat tttgtcgtta tctgttacca tatattgtga taagtaacca
     8821 cgatcaaact gcataccttc tacaacatct agttctgttt cgatcccttt tgattcttcg
     8881 attgtgatga caccgtcgtt tccaactttt tccattgcat cagcaatcaa atgtccaact
     8941 ttatcagaac ccgaagaaac agctgctact tgtgcaatgg cttcttttga atcaacaaca
     9001 gttgagatat tatgcaattc ttcgactgct gcttttgttg ccaattcgat cccgcgacga
     9061 atgcctagag gattagcacc tgcagttacg ttttttaaac cttcgcgaac gattgcttgt
     9121 gtcagaacag ttgctgtcgt tgtaccgtct cctgcaatat catttgtttt tgatgctact
     9181 tctgatacaa gttttgcacc catgttttca aaatgatctt ctaattcgat ttcttttgcg
     9241 atcgtcacac catcatttgt gatcaatgga gagccgtaag atttttctag tacgacattt
     9301 cggcctttag gtcctaatgt tactttcact gtatctgcta atttatctac cccgcgtaac
     9361 atagctgcgc gtgcgtcttc tgcaaatttc aattcttttg ccatgattga ttcacctcat
     9421 tgattatttt tgattatttt tttatcttcc aaatttactc aacaatcgcc atgatatctt
     9481 ttcctgcgac gatcagatat tcttttcctt cgtattttac ttctgtaccg gcgtattttt
     9541 cgaacataac ttggtcgcct gcttttacag cggcaggaac tttttcaccg ttttctaaca
     9601 aacggccttc accgactgca acaactgtcc cagtttgggg tttctcttta gcagcagaag
     9661 ctaaaacgat tcctccgaca gttttttctt cttcttttgc gacttcgata atgacgcgat
     9721 cacctaatgg ttttaacacg ataaatccct ccgataactc tttttttaat ctgttagcac
     9781 tcttaacctt cgagtgctaa cctacatttt taataatact cattctccct cttttttgca
     9841 agcttttaaa tcaaagaaaa gaaattttat tctgacagct gcttattgaa attatgatat
     9901 aattgaaatc aatcgatata agcttgcctt gcatgcttgt tatcccttaa aaggaggaat
     9961 tacatgtctt taaaaaaata tagcttcctc agcatttttt gctatggtct aatttttatc
    10021 tcacccttga tcctagcttc gataggactt gtaaaatcta cgtctgaatt gatcacagca
    10081 actgcagtca tgtatatact aggcgccgtt gtcctagcta ttttttattt taagcaacgt
    10141 gaacccttag cgatcgaatc agcggtaaaa aaagcttctg ctccaaagat tgttatttat
    10201 ggattggttg gaatctttgt cgcattgatt ctgcaaagca tcgctgtcat gttagaatct
    10261 gtattgtttg gcaaagcaac cccttccgag aatacacaaa atatcatcca gatgatcatg
    10321 gaagcacccg cttttatttt ggcgacaact gttgctggac ccatcatgga agagttcgta
    10381 ttccgccgca gtatactggg gatcatcagt cgatattcaa atttttgggt cggagcagtg
    10441 atcagctctt tgctgtttgc gtttgctcat aatgatggtc atctgctaat ctactttttc
    10501 ctaggtttct tcttcagcct agagtacaaa gcaactggtc ggatctggac atcgatgatc
    10561 acacacgtag gaatgaacac attagttgtt cttgttcaat tagctgtcca aaaagggcta
    10621 gtataaacat gactataaat actcctgtca tcctagattg gtgattttcc caatctaaag
    10681 cgacaggagt atttttgttt tttagtcgtt catcttttct gctgctccat cattttttgt
    10741 ctgtctttcc tttttgcagt ttgcagaatc tatttttttc aaaaatggga aataaatgaa
    10801 aaaactaatt gacaacacga taccttgtaa aatcgctgca ggcactccgc ctactaagaa
    10861 acccgagagg atcggcggtg ttgtccaagg gacttggact gctgtaaata aaggaacgaa
    10921 tccaaaatat aaagcggaat aggtaatcaa tcctgaaacg accggtgcga aaataaaagg
    10981 aacagccatc atcgggttca tcacgatcgg tgtggaaaaa ataattgatt cattgatgtt
    11041 gaaaaaggct ggtaaaagag caagatttcc taattctctg tatttcttcg atttcgcaaa
    11101 aaccgtcata aaaaatacag cccctaacgt caatccagca cccgtaaccg tcatgaactg
    11161 atcgagaaat tgctgagtaa cgatatgtcc gccatttgcc accgtcaact cttgtcctga
    11221 ggctaagatt tctgcatttg ctaatgaatt cgcttgcaag acgggactaa ccaacccatt
    11281 gaccatcgca gatccatgaa gaccaaataa ccataaaaac ggaacaaaaa aacctaataa
    11341 aagcacacca aacaacgtgt ctatgaacga ttgaatggga agctggacat atgtccatac
    11401 tccttgaaca acagtcattc tgaacaaatg ttgaaaaacc gctgaaccga taagcgtaaa
    11461 agtgactata acaatgattg gaatcaatga agtaaaagac tgccaaacat tttcaggtat
    11521 cttctctctt tgcttccacc ctttcgcaag tcttactaaa cgtgcatagc cccatccgat
    11581 gatgagccca gttgtaactg ctcctagtat cccttcagca cctgtccaga cagtatctat
    11641 ctgagtattt agtggctgaa taagcagtag ataagcagac aaggctgtta tgccgactgg
    11701 taagcgatcg atgtcttctt cttttgcata agcgtatgca atggcaaaaa cgatccaaaa
    11761 ggctatcaca tcaaatgtag cctggtatac ttgtgtaaaa tacatagaaa atcctgtttt
    11821 gtctatccac tgagcgatgt aatccacagg aaaatgagca aataagaaaa acagcgagcc
    11881 aacaataatt attgggatcg tgtaagtcat tccattacgc aaagcagtta agggtctaag
    11941 attcacaaca gatgttatca ttggtaaaaa ggcacgatct aaccactctt tcatttctta
    12001 tctccttcaa cttctatttt tatcaatcag ttttcttaat ttaactatac ccaatgatca
    12061 cgaaccatga aaggaatgcg cactgagctt ttcaaaaatc ggattctttg acgaatattc
    12121 agcaactgct tacaatggac aggaataaaa aaggaggttt tttatgaaaa ttgcactcat
    12181 tggagctact ggccatgcag gttcatttat tttagacgaa gcattgtcca gagatttaga
    12241 cgtgacggca atcgtgcgtc atccagaaaa attgccatca gatgttccgt ttatacaaaa
    12301 ggatttattt gagttgacaa ctactgatct tgcatctttt gatgttgtcg ttgatgcttt
    12361 caacgctccc aaaggaaaag aagaaatgca tcaaacttct ttgtcccatt tgattgagat
    12421 gcttgaagga accgatacac gcttaattgt cgttgggggt gcctcttcgt tattcttaga
    12481 tacagaaaag acgactcgta tgattgacca agtacctaaa aatgcaccct tctatccaac
    12541 agctttcaat atgtacgaag gattgttaaa attgaaacaa tctaaaaatc tacattggac
    12601 ttatatcagt ccagcttctt tatttgatcc acatggcaaa cgtacaggta cttatttgtt
    12661 aagtgatgat tatttacaaa gaaacgctgc tagcgacagt acgatcagca tggcagatta
    12721 cgcgattgca ttagtagatg agatactgga tgagaaacat gaaaagcagc acatcagtgt
    12781 agtcagtcga taatacggtt atttttttaa tatgctaaaa aaataaaaat agaagataaa
    12841 accaaccctg attgatttta tcttctgctt tttatatttt tattgattga actgtttaaa
    12901 tcgaaaatag ctcagttgct tcatctggat ctgtatcata gatttcaaaa tcatgtccaa
    12961 cgccaaaatc tttttctttc aaaacatttt ccattgttaa atgagcaagt tctttcatat
    13021 tgttttcctt gctaagatgg ccaagataga ttcgtttggt ccgattaccg atcacatcag
    13081 tcatgacaag tgcaccgtct tcgttggaaa gatgtcctct atctcctaaa atacgctgtt
    13141 ttaaattcca cggataaggt cccatgcgta acatctcaag atcatgatta ctttccatca
    13201 aataaccatc ggcattttca atgacacctc gcatataatc actgcaatat ccggtgtccg
    13261 tcaacatgac gaatgaccga ttttctttat ggaaacgata aaattgcgga gccgctgcgt
    13321 catgggagac gccgaaactc tcaatatcga tatcgccgaa tgttctgact ttccccatct
    13381 caaatagatg tttttgttcc actggtacat ttccaatcag tggttccatc gcttcccaag
    13441 tctgctgatt ggcaaaaaca tccaaatgat acttacgagc caagacaccc acgccatgta
    13501 tatgatctcg atgttcatgc gtcacaagta ttccatctaa ttggtccggt gtccgatcaa
    13561 tttgagccat caaggaagtg atttttttac cgctaagtcc tgcatcgacc aaaagccgtt
    13621 ttttttcact ttcgatataa agtgaattac ccgaacttcc acttgctagg atactgattt
    13681 taaaagctgg tttttcttca gccatttgac tcctacttcc cttcttactt tgtaaacttt
    13741 tttcattata ctcgcttttt tattgatctt ccacttttgg tacagtattg tttgtcacga
    13801 tcgtattgct gaaagcgttg acgctctcaa tttggaccac tttatcgctt gtttcgatcg
    13861 ctacatgcca aacaggaaca tacacatttt tttcccggac tttcagaata caggaataag
    13921 ctagctgacg ccataaaatt tttgatcctc ttggtatttt gttattgatg tacaatgtat
    13981 tgattgcttc ctcttccgta tacagttccg tcttttcgcg cagctgctcg atatcagata
    14041 aatgcgtctg catgtatttc gtgatgctat aatcattatc gtttttttct aaagaaagaa
    14101 cgatttttgc tgtatcatca ttgaatggga tgccttcata ctcttggctt gcttggattt
    14161 ctagtggttc ttcttctaaa tgagaaaagt ttttcgtgta cacataatca ttaccgaata
    14221 aaatattgtc tttgttagat aaaaaatctt ccaaggaact actaatttta tctggatcga
    14281 tcacgttctt ttcagcatct gaactggata aagatttcgt caaaacatca ccgtctaaaa
    14341 acacaccact tgccagcaag ccgcttttac cagccttgcg ataattcgct aatgaagtgc
    14401 taagattggt ctgctccgcg ctaagataat agccttgctt gttctcagac gaaatcttcc
    14461 ccgtgtaagt gatatcgtcg ctagccagac gttgttggat tgattcagtc tgattagagc
    14521 gaatgacatt gttttcttgg tataccgcgt catgataact gctgaataaa aacacattca
    14581 accctaaaaa agcgacgaaa aatatccatt cgattttttt gaaatccaat taattcgcct
    14641 ccaatccagg taatttttcc agaaggtcgt tagctgaata ccagttatct ttatatttca
    14701 cataccattc aggtaagaga tcaacgacac gattcgtttc tttgacattt tgccatgtat
    14761 agccaacgat cactgaacca atcttgttgc tatctgctcc cttagctttt aactgctgga
    14821 tgatttcatt cgtacttgca agttctactt cttcgtctga aggtatcggc acttgaatcg
    14881 tattcaaact ggtttggata ttcacttgca cagaaccatt cgtttcaccg ccaacttcca
    14941 caccaatctg tcctctgctg tctgtcccaa aaaccgggaa gccttccacg aatatccgat
    15001 aatcgatcgt attgtcactg cggtcaaaat atctgaggtt ccctaaagaa ctgcctaatt
    15061 tgctaatata ggggaaactt tcagaaaata cactagcatt ttcttttttc tctggcagct
    15121 ctccacgaaa atcaactact tgcgtttccg aatggataga cattgtttca ttcccaccgt
    15181 aaaaaatcaa ttcattgctt tcatcattat ttttcacgct atctggtttc tggaaaaaag
    15241 catttctaaa aagtgtatag ggttgctgtg acaggatata actatacttc ttcagtttga
    15301 tcgaatgttt cgtagcatat tgcgtgtcga tcgtcgatgg ttctgttgtc atttctgtcc
    15361 attttgtgtc ttttgctgtt aattctttat tgatatctgt ccaattcaac gaaatatttg
    15421 cttcatatac gagatggttc gaataattca aaaatcggat ttttttattt ccttgatcga
    15481 attgtattct agtaaaatac atattttcgc tttcttttgc caagttcgac acatctaaat
    15541 ctaaatcaaa tgcttccttg tattctctta gcaaaaaagg cgcattatag gccaactcaa
    15601 aaccagagtt cagatttttc acctgttcaa actgtgattt gtctcctgaa actacttcag
    15661 aaagctttcc aaatcttgct ttatcgatga tcaattgcaa tcggtacacg agattttcac
    15721 tgttggtttc ttggatcatt cccgaatggt gccatgtaac atgaagcgga agaaagactt
    15781 cggaagtttt tcgattgctt tgagactcga tcatttgagt gtttgtctca tctacgttga
    15841 ttgaactttt tcctgctggg ctcagccaga ttgcataaga gaagtatatg cttaacgcaa
    15901 ccatcaaaat gagtcctata cgaacgactt tttcagagat tctcattccc accattcctc
    15961 ctcataaggt tcatatggca gcgagatata aaaagtcgac ccttttcctt ccttgctctc
    16021 tgcccaaata gcgcctcgat gcgccttaat cacttctcta gaaatagcta atccaagtcc
    16081 tgtcccacct tgtgctcttg cacgtgcttt atctactcga tagaaccgct cgaacactcg
    16141 gttcagatct tttttaggaa tccccaatcc ttgatcggtg atacttaaaa tcacgttatt
    16201 atgcgtttct aataagcgac aagtaatcgt tcccccatct ggtgaatact tgatcgcgtt
    16261 attcatgata ttatcgacta cttggatgat tttatctgta tctacttcga cccaaagatc
    16321 tctttgcgta aattcacgac ggatcttata atttttttcc tgatttccca ccatcatatc
    16381 aaaccggtca agaacaaaat tcactaactc gttgaaattg acatattcta attgaagctg
    16441 ggtattacct gtatccatgc gagatagatt taagaggtca ttgatcattc gaatcattcg
    16501 gtctgtttca tctagcgtta ccttcaaaaa atttggtgct acttcctcgt ctttccacgc
    16561 accttcgctc aatgcttcga tataactacg catacttgtc aacggtgtac gaagttcatg
    16621 ggaaacatta gagacgaatt cacgacgttc ccgttctgtt ttttcttgtt ctgtcacatc
    16681 atgcaagaca gctactaatc cgctgataaa acctgattcg cgacggatca tagaaaagtc
    16741 tactcgcaag atcatctgat cactttccaa tgtactggtt gaacggtcaa gtaccatttc
    16801 gtttggatct tctaataatt tccgcaacgt atattcttct tcgatatcta aaagtgtcaa
    16861 aatcgattgt ccgattgctt cctcgttttt gatatctagc aaggaaacag ccatatcatt
    16921 gatcgtaatg actttacccc tgcgatctgt cgcaatgacg ccgtctgtca tatgggatag
    16981 cacgctatcc aaacgattgc gttcagcttc cattgtatct tgagcttctt caatacgttc
    17041 agctaactga ttgaaggtat cagccagctg tccaagttca tcttgtccat gcactttgac
    17101 tttccgactg tagtctcctc tagcgattcg aatggcttgt tctctcattt caccgatcgg
    17161 ctgtgtaatc gaacgagcaa ctaaaacaga tacgatcatt gaaataaatg ccgcaattag
    17221 tgaggcagta aagaaaatga ctgctgtgtt attgatttcc gaatattttt gttcgatatt
    17281 acttttgacg taaagagccc cgataacggc atcgccggtc ggggacagga tcggttgaac
    17341 attgatattc acccgtttat cgtcgttatc taaagcaaca taacgtttag tcgtgaaatc
    17401 attgatatca acataatcat ttttctttcc cacagcgctt tgttcattga catcggttgt
    17461 tcctcggatg atcccttttt cgtcaacgac ccgtatttcg atgatatcgc tagtcgcact
    17521 gttttcaata aatcttttga tattcgcatt tacctcttca tcatctgcat tatcttttcg
    17581 attcaattcc gggctgattg ttgttgctaa tgattccacc gtttgattca tatctttaat
    17641 aaatgtattg atcgtggaac gttctaaacc acgtataaag taggcaccaa taatttcgat
    17701 cgagatcaat aaaatcagaa taaaggtaat agcgatctta aaattgactg atcgaaaaaa
    17761 gcggactttt cttttcatcc aattactcct gttctgggtt acgcaaatag taaccgacac
    17821 cacgacgtgt cactaaataa tttggatggc ttggattatc ttcgatcttt tcacgcaaac
    17881 gacgtacagt tacatccact gttcgtacat ctccaaaata atcataaccc caaaccgttt
    17941 gtaataaatg ttctcttgtc atcacttgtc caaggtgctt cgccaagtaa aagagtagtt
    18001 cgaattcacg atgtgttaac tcgattgttt ctcctcgttt cgtcaccatg taagcttctg
    18061 gatggatcgt caaatcacca atcgtcaatt ccgcaggtgc tgcctcttct ggttccttcg
    18121 cagccgtggc accgcgacga agatttgctt tgacacgtgc aactaattct cggttggaaa
    18181 atggcttggt cacgtaatca tcggcgccta actctaagcc taggacttta tcaatttcag
    18241 aatctttggc tgttaccata atgatcggca tatcataagt tttgcgtacc tcacgagcaa
    18301 cttctaagcc gtccatttta ggtagcatca aatcaagtaa aatcaaatca ggttctacct
    18361 cagcaacttt ttccaaggct tcttccccat catatgcagt ataaacgtca tagccttctt
    18421 tggctaaatt gaatttgacg atatccgaaa tcggcttctc atcatcgaca actaaaactt
    18481 ttttcataaa gagccacctc ttatatatta ttgtcatacc ttctcattat accgtatttc
    18541 tgcttttgtt tcatctttgc ctataaaaca aaagagaaaa ttaaaatgaa agtgcaaaat
    18601 actttattat tttttgttct aacttcaata aaaaaggaaa acactgattt tcacctcagt
    18661 gtttcctttt tttgatttat ccttctaaaa cttgtacacc gacacccaat aatcccggac
    18721 cagtgtggac tactaatgcc ggagaaatct ggccaaagta gatttggttt gcttgtggga
    18781 actctctttt aagtctcgct tccatttcaa ttgcttcttc tttcgcatcc ccatgggcaa
    18841 cagctaagtt gaagaccttt gcattaccga ttttttcttt gacgtaagca accgttttat
    18901 ccaaactttt tttacgtccc ctagcttttc ctactgtgta ataaatccct tcttcattac
    18961 atgagatgat tgggttaagc tttaaagcgg ttcctacaat agaagcaact aaaccgatcc
    19021 gtccaccttt ttgaagatat tccaatgtcg caacattaaa gaaaactttt gttctttcga
    19081 ctaactcatt cagattactg atcagttgat cccattctac tccttcttcc agccattttg
    19141 ctgcttggat cgcagaaaaa cctgcaccaa taccaatatt tttcgtatct agtacatatg
    19201 cttccaattc ttcttgctgt tgtgcaatta aacgaaccac attatatgtc ccgctcaatc
    19261 cactggaaat tgttaccgca agtacctttt cataaccgtc ttttttgatt tgctcaaaga
    19321 tttctgtgat cgactcacca tccggcaaag atgtaccagg gatttcctct ggcaaacgtt
    19381 gatagatctc ttcaggggta atgtcaattt tatctgtata tacctgatct ttataaataa
    19441 ttttcaatgg gatcatatac atctcgtatt tttttatcaa ttcttctgga acatccgtac
    19501 ctgaatctac taaaattgcg attttattgt ttttcatttg ttttctcctc ttttaaatat
    19561 tcggtttcta gaacaatcgt tttttccgca agaagtttca ttgcaaacga taaagttgcc
    19621 gctcttacag caatatattc tacaggggtt gcctggtcga atttttgcgg atgagattta
    19681 ccttgtgcta attggctgac catccacaca gctgcttctt gttcatcgca aaatagatta
    19741 taggcttcac gaattcctac tgtttttcct tggaacaata tcccttcttt gatttcagga
    19801 atagtcaata cttgtttcaa cgtagtgatt gcaagtaaaa atgctacgtg ttctttgtta
    19861 tatctttttt taactggtgc tggtatcaaa cctagtttaa cgtaattgtt taccatagaa
    19921 gaggtaagca atggatgctt ttctccttgt atcacaggtg aaagataacg atcaactaac
    19981 gttctgactt ggtccatata aagttctaag tcagggagtt cttcccagtg aggcagacgg
    20041 aatgtttcta gttccttccc ccactccatt aattgctgac gcattttttc catcataacc
    20101 gctccttttt tcatttcata aacatgatat cagacttatg ataaacattc aatctagttg
    20161 ctttaactag gtgatttgat tagcgggatt ccatgaaaac aaaaaacaag aaggctaagc
    20221 cttcttgtct tttatcgtta agaacaggag acccgccggg gagacggcgg gaaaggagta
    20281 aaaaatgaaa aaagtgttag ggtttgttct cttgcaagaa ctttgtttgt tggtatgctt
    20341 atataatagc gagcaaatgt gaataatcta tgaatgattt cctataagga agtaaagatt
    20401 tttctgattt tctttaaatg tagtcttgca ttttaaaatt cttagtgcta ctattattaa
    20461 gtactttttg tacgggccgt tagctcagtt ggtagagcag ctgactctta accaaatgct
    20521 ctatttgtga aaaaagtttt ataaagcttt ataaataggt ttttagtttg tgaatttttt
    20581 cattgccaac atactgccaa caaattgcca acaaaaaaat tctaaaaaca agaagcgata
    20641 ccccaaagat attgtttttt gtttttttat tctttaaacc caaatgcgtt ttctaatgct
    20701 tctgctgcac gatcttttac tcctgcggtt aaatgcgtgt ataaattagc agttgtcgaa
    20761 tacgatgcat gtcccagcca ttcttgaatg gatttcatat tctggccact atctaacatt
    20821 attgaagcat tagaatgacg caagtcatga aagcgaattt ttttcaatcc atttttctta
    20881 atcaacttac tcaaacgatg cgttacatat tgaggcttca tcaaccgtcc atcttccata
    20941 gtaaatacgt actcattatc ttctaaaata taatcatctc ctaaaagaat acgattatct
    21001 tgttgccatt tagcttgatt tactaatgct cgttcaatcg tttcaaccaa aggtaatgta
    21061 cgatagctgg aagagttctt tgtttgatct ttctttatta actgtgacat attctctgga
    21121 ttttctatga ttacatggtt tatcatgatt tttttgttta aaaaatcaat agcagaccat
    21181 tttaatccta aaacttcact tcttcgcaat ccataaaatg ctgttaaaag aactaccaaa
    21241 tagagttttt cttctgacaa aatgtcaagt aatttcaaga tctcttgttg attataaact
    21301 tgagaaacaa attgtttctt ttttggcttt cttactttat ctgctacatt tgtacttatc
    21361 aattctgtaa taacagcctc ttgaagagct ttgcgtataa atgcgtgata atgaataacc
    21421 gtatttgctg tacagttatc atttaacata tattgataaa actcttgtaa atgataaggc
    21481 tttaacctat ttagtttaat ttttttagga taaaaaaact tgtaaattcg attatggacg
    21541 taatgcttat acccaacata tgtgttaaat gctacttgtt tttttgatac ttccaaataa
    21601 ttaagcaaat agtcataaaa caataaattt ggattagcac attcacattc catagctaat
    21661 tgactttcca attctagtaa aaattcatct ctaatttttt ctgctcgttt tttatttcct
    21721 ttctttttta attttgtatc tttccacttt tgtctaattt ttccgttaga gtctttataa
    21781 gatacaacta cttgtaatgt tcctcttttt tcaattatgt ttgctcttaa tttcaattca
    21841 atctctcctc ttaaactgaa atatcggcaa aaattttgac tattttatta tagcatgatt
    21901 tttaccttca tcttgtattt tcaatatata ctcaatgaca gcttttttag gtattttgta
    21961 ccttgctcca atttttatat tttctattga tctatctttc agcaaattat aagctttatt
    22021 ttttccaata tttaacattt tagttaaatc agtaattttc aatatcgatg gataactttc
    22081 aaatattttt tcataatctt gaaaatccat aaaattcact ctcaattctg ataataagta
    22141 ttgcactttt ttattttagg tgttagaata acaaagtatt aatttgggct gttagctcag
    22201 ttggtagagc agatgactct taatcatcgg gtcgcgggtt cgagcccctc acagcccatt
    22261 gggtgccaaa cccttaaata cagtgtaaac gttggtgctt agcatttaga aaacgtatat
    22321 actgtatttt tttgttttca aaagagactg tagtacccct ttttttcact aaaagcccac
    22381 tgtagtcatt gatatttttg ataagaaagg aacataatta tgcgtaatga aattgctaat
    22441 ataacaataa tccgagaaac tttacaaaac catactgcaa atgagatctc taaacataca
    22501 ggattaaatt tgagtactat aaaaaaacta aaaagtggag aacgttcaat agaaaaacta
    22561 aatttacatg atgctatttg cctcactgaa tttggtctta aaaataatag aaaaaatgtt
    22621 gaaatcaaca tatggaaata attcttaatc agctgctttt gtcaaaagca cataggaatt
    22681 tcacctctct ccaagtttac ggcgagcctt acggaagtat cattatccca acttcaaatc
    22741 atcgcttttt attgactata aaaattattt tagaatgttt atcgctcctt ctttacagta
    22801 agaaatcatg gcgcaactaa aatatgctcc ttgaagtgtt aacgtactga tagaatgtat
    22861 ttaaatttca atgatcaata ggttttctgt ttaaaagaat ctactaatcc tttaactatt
    22921 agaagattct tttaaacaga ataagtggaa aggggacttt ttagttagtt aattttaaat
    22981 tttaagatcg cttctatcaa tttaatttct agctgtctta gcatatactc atctacatag
    23041 gtaataaatt gccccgcctc attaaatcca tttgtcaaac ataacttagc tatataacta
    23101 tgataatgtt tgagtatcat cttgattgaa attggatctt cttctagagc agattggata
    23161 atggatagag gaactaatgg atactgccgt tttttggggt tgtctaccat aagtcatacc
    23221 ccatttttaa ttgttcaact gccttctttc gttgatacca gataccactt gtactcatat
    23281 tgaggatttt accgatttca gtatcattaa aacctgcaaa ataataaagt aggataacct
    23341 ttcttcttat ttccgataaa tgatcaatgg ctgccgataa cttttgatct gaaattaata
    23401 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gagtacttac
    23461 cttcatttga attcgtcaat ggttcctcat tatctgttat gttaatgttt cgttttcgaa
    23521 tagacttagt ttcgtttcgt aatacttttt tacagtaggc atcaaattga tgttgtatag
    23581 atttctcatt gtaattttgt tgcatgataa atatgctcct tttttcccct ttccactaat
    23641 acaacaatca gaaagcgcct ttttttcgaa aaaactagaa aaaaatatcc ttctttaaac
    23701 agaaaatctt tttataaaaa aaaggagttg gcaattaagc agccactcct tttttatatt
    23761 ccttataaaa ggtaaattat tttttataac tgaaatttgg tattgatgac catcgtttct
    23821 ttagatagtg agcaatcatt ttaggctttc tatctctagt aaagattcct ttcttatttc
    23881 cttgcactct taaaatccct tgacttgttt caaaatctgc aaaattccac acttgttcac
    23941 caacaaaatt gtcacattta tctattactt cgtgatttgc ttcgtaataa cccacttgat
    24001 actcttctgt aaacattgtt ggtactgtat catgaagtcc agctactgta tctgctccat
    24061 attctgtaaa cattattggc gtatcgggac atctttccat ccatccatct agctcttttt
    24121 tcaacagata ttttgcatgt tttaggtctc cacctgaaac ataccaacca taatagcgat
    24181 ttaaacataa aacatcgaca atatctccta ctttacaaat atctggtgtt gactttaagt
    24241 aagtaacgat tgttacaggt ctcttctgag ggtcaagttc tttagtcaat tcaactaaag
    24301 gttcaaaata ttctttagcg ccttcagaat ccgaatctgg ttcatttgca atagaccaca
    24361 ttacgacact cggatggttc ttgtcgcgtt ctatcaattc cttaatcgcc attttgtgag
    24421 cattttctgt ccgtatttct ttccaagtat catgtttttc tacgttgtca tctaataaag
    24481 tagccatgaa attcaaatgt aatcctactg caggtacttc atcaataact acaatacctt
    24541 ctcgatctgc taagcgcata atttcttctg aataaggata atgtgaagtc ctaaatgaat
    24601 tagcaccgat ccatttcatt aaattaaaat ctttgatatt tactgcttca ttcaaaccac
    24661 gaccattaat atacgtgtcc tcatgcttcc caaatccctt aaagtaaaaa ggtttattat
    24721 taatcaaaaa cttcccattc gaaacctgaa tactacgaat gccaaattct tctgtgtacg
    24781 tatcaatcac tttattttcg ttaattaaac taactttcat actatataag taagcattta
    24841 gaggctccca aagtgttggg ttcttaagtt gaatcactcc ttctttcccc ttagcttctg
    24901 ctataagttg agcttcttcg tcatagattg ctattagaat ttctccggtc cattctgtat
    24961 cgatattaaa ttctacattt gcatctgaat caattatttt tgtattaata gtgacatcct
    25021 ttatatagtc tttcggagtt gtatacaatt taacgggtct attaatgcct gcataattga
    25081 aaaagtcaaa attaaccaaa ttttcttttt ttatgtctcc atcttcctgt atcgtttctt
    25141 tcaaaatccc tacaggtaaa gtgctatcgt caataacatt gtttacagcc actgtaattc
    25201 tgttacttcc atcttttata aactgattaa tttctgattc aaatggcgta aaccctcctt
    25261 tgtgttgatt aatcaatttt ccattcaaaa atacttttgc ttcatgtgtt gcagatccaa
    25321 aacgaagaac taatctttct tctaataatt ttttaggaag aatcacttct ttttcatacc
    25381 acacccatcc aacatgattt ctaatgttct ctgtttcaaa taaatcatta taagaagaag
    25441 gaactgaaat tggataactc tctttttcaa ccattggttc taaagcaagt tcttcttgta
    25501 agtcatcatt tgtatctaac ttaaatctcc aaagaccgct taaatcaatt acttgcctcg
    25561 tctctgtcat aatgggatat aacatgtaaa tcactcctat ataatttaaa tattaaataa
    25621 atgtatcctt taacccacaa taagcatcta ttggacttat tcccttaaat tcagaaaggc
    25681 ccattagttt gtccactaat atttctaaag tttcttgttt tccatcataa gtattgatat
    25741 aagtttttac ttgaggcaca tcagctaaat gaaacggaca ctgtacagat acaaaaattg
    25801 ttggaatttc atgaatataa aatggaatat ccggagtacc tttacttgct ggccaaacaa
    25861 ttctttgaac tgtacctggg ttaacattag ctatattaat aatcaagtca tatttttcag
    25921 ttaaatcttt gattggtctc ttagcagcat atattccagc tatattttta attttttctt
    25981 cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact tcaaatcctt
    26041 ttgctgacaa aatctgtttc aataaatcaa tggcctgagg tctatttcca gcaactactt
    26101 caccaaaccc accttttacc ccatgtacat ctaccagtaa aatcctttta tatttttctg
    26161 gtacaatagg ccaaatatct tgtttatctt taactagtgt aattgcttga tcagctacct
    26221 ctttaaaaat tgatttattt tcaattaaac caattttttc catagcttct tgtttcggta
    26281 acaaaatttt attcttttct tttttatgca aacctagttt agcctttaag cctaaaattc
    26341 ttcttaatgc atcacgtaaa cgctcttctg aaataattcc tttgtaataa ccttccatca
    26401 tataattaaa gtcttcatct ggatcattaa aaaataggaa tagatcacat ccagccgcaa
    26461 tagaagtggg caataattct gaccgtttca ttgccccagt cattcctaac atatgactag
    26521 catcagtaac tactaatcca ttaaacccga gttttcctct aagtaagtca gtgattaatt
    26581 cttttgataa agttgcagga agtaaatctt catctttcag atcaagatta aaatgttttg
    26641 aataagctgg catatggata tgacctgcca taattgaagg taatccagct tctattaagc
    26701 ctgagtatac ttttccaaaa gaattatccc attcttctgt acttaaacta ttaatactaa
    26761 aagatagatg ttgatctcgt tcgtcaatac cgtcaccagg gaaatgttta gctgcaggca
    26821 aaattccact ctcttgaata cctttcatat aagctaaact aagttctaaa actttatcag
    26881 ggctttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta ttaatatcta
    26941 caataggagc aaaactccag ttacatccaa tagccgatgc ttcaattcca gaaactctcc
    27001 ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct acaccaactt
    27061 cggtaccatc tgtacaagct ccgttaccac ctgcttctgt attagcagca attaataaag
    27121 gaattttgct ttctgtttgt aggatatagt tttgatcata tacctcatca gcagttcctg
    27181 gattatatcg tacagctcca atatggtact gattgaccat actacttaag taatttttat
    27241 cacgactaga tcccatatta acaaataatt gacctatttt ttcttctaaa gtcatagcat
    27301 caatcgtctc ttcaatccaa gaaatagcat cttcatctaa atgatatggt ttttttcgta
    27361 agtctacttt tatagtcatt ttctttctcc tctacttatt agtaagattt ttccaaaaag
    27421 tatctatata tttttgttca atatctatat actctcccga taaacattta aataaaatat
    27481 cagttcttaa gttttcccat gattcttttt ctttattaac caaaatagga taaaagaaaa
    27541 ctttattctt atttgcagct tctagatcac caggagaatc accaatcatt aatatcttat
    27601 ccaaatcaca accattttca attacacttc gaataatgtc ttcttttttt cctttatctt
    27661 gacaacacag ttcatcaact aaggataata attcatgtct ttcccattct tcttgaatag
    27721 cttccttatt agctgagctt acaataatta tcttcccata ttttttcaga gcttttatag
    27781 tatctctagc acttaaaaag ggtttatcta atcctttagc ttgactaatc tgtcgattga
    27841 cttcttccga ccattttaaa gcttttaata aatcctctga ctgagagtat tctatttccc
    27901 tcttcaaaga tggattagac aattcttttg ttacagatac ccagtgcttt aatcgattaa
    27961 tcgatggtac atcatttttt tctaatgttt taactaaacc taaaaatctg tttattccac
    28021 gagtctctga atataaatta atattattcc aatcctgtag gaatttttcc ctatgatgaa
    28081 catcaaaaat ctctgcagca attggcccga aaaagagatt gtgtttataa gtcattgtat
    28141 ccatgacaca accatctgaa tcaatacaaa atatccaact gttcatgttt tttctcctag
    28201 ttatgttttt gatttagcaa attataattt tctaatactt tttctttaga taatggatat
    28261 acgaaaaata aaatcaatcc tacaacaaag aaacaaactg ctggaatcaa cgttgaagag
    28321 ttatacagct tttgtaaaac ttctccactt tgtacttttg ctaacgaatc ataccctatt
    28381 atagaaagag tccaacctcc gacaccgcca gctaaagctt gtccaatttt tcgggcaaaa
    28441 gaatatactg cataaatagt tccatcttct ctttggccag atttcacttc ttgatcatcg
    28501 attacatcag taatattcgc ccaaatgact gtattaaaaa tacctaaccc catatatcca
    28561 acagaagtaa gaactaagaa tacatacata ttactaggct ttatcaaaaa taaaagtaaa
    28621 tatacaatag cagagaagaa catccctact gacgccaatt cttttttacc gtatttttta
    28681 ctgaaaatta aacttacagg tgaaacaagt accaacatca aaataggatt gataaagttc
    28741 attaaaacta ttccctgtgc attattataa taatctggaa atacataatt gttcatactc
    28801 attgttaaaa gctgagcaag caacaaaaaa atagctgcag ctattataga aagcaacgct
    28861 ctatttttag ctaatccttt taaagatttc aacaatgaaa cttttaactg cgattcatca
    28921 cttttaaccc gttctacagt taatttataa catattacgt aacaaataac tgcacacact
    28981 gaaaatactc ctgccacaat tgtaaaagtt gatccaccac gtacaatttg agctccagaa
    29041 ctatctcttg tataaataaa aattggtgtt aacacgccaa tgaacatacc tgctaaggta
    29101 gctcctatac tacgaaatgt tgataacgct gtcctttctt ctgcaacaga agtgatagct
    29161 gatgccattg aaccgtaagg aatattaata gatgtataaa aaatacttcc ccatagtata
    29221 tatgttacat acatatatat aactttaata ctcatagaag ctgatgccat tgatgtttga
    29281 tacattaaaa aacttgctaa agcgactgga ccactcatcc gtaaaatcca cggtttaaat
    29341 ttgccagttt tagatggttt tgatccatcc acaatacgcc ccattgttat gtctgtaact
    29401 gcatcaataa atcttgctac taaaaataat gttcctacgg tagctccgct tatccccaat
    29461 acttttgtat aaaaaatcat taaaaaagaa ctagcaaaaa tgaaagtgaa atcattccca
    29521 aaatctccaa acatatatcc tattttgtct cttagaccaa attttctttc catataattt
    29581 gcgctccttc ctattgttaa gcgttttcat tctgttgaac tgccaactta cgtactatac
    29641 tctgaagtat aaagagttag tatttatttt ttacatttta tagtattatt tattttttct
    29701 cctaataatt ctttatctca taaaacttat ctatatattt actagctatt cgagttactt
    29761 cttcaaaatc acctttttca acaggagcca acaaattccc acctacacca actgcaacaa
    29821 cgcctgcttc aaaccactct tctaagttat ctagacttac tccgccagta ggcattatat
    29881 ttacatatgg taatggagct tgatacgttt ttacaacaga tggaccaaaa gcagaacctg
    29941 gaaatagttt aatgatttct actccagcgg taagtgcctc ctttatctca gtaattgtca
    30001 tacaacctgg taaataagga atttgatata aattacaaat ctctgctgtt tgtcgatcaa
    30061 aagatggact tacgatatat tctgctccag ccataattgc aatacgagca gtaaacgcat
    30121 ctaaaactgt accggctcca attattacat ctttttgatc agcatatttt ttagcaattt
    30181 ctttgattat ttcttcagca ttcggaactg taaaagttag ttcaatccct ttaattccac
    30241 ccgcagtaat tgcatttatt gctttaatgg cctcatcttt attttttcca cgtacaaccg
    30301 caatgactcc agccgcttct aacttactta aaatttctac ttttttcata tctatctcct
    30361 tcttttatgg atacatactc tagctagttg tactaactta caataaataa atttaagctt
    30421 ttattttttg tattattttc tactttcgat ttcaaaaaaa ttaaaataat tatacgcatt
    30481 acgataacaa atattctcca ctatctgact aagatacatt tcatcatctg gaattcgacc
    30541 tgctttaacc cactcgctta gtaattgaca taatactcga cggaaatact catggcgagg
    30601 atagcttaga aaacttcgag aatcagtcaa catacctgta aaattcgaca acaaactttg
    30661 ttgcgccatc gtagtcaact gttgacgcat tccttcagct gtatcattga accaccaagc
    30721 acatcccaat tggatttttt gaatcatacc cccttggaag ctttgcattg ctgttgctag
    30781 tggaagccaa tcggttggat ttaatgaata taaaattgtt tttggtaagc acccactctt
    30841 ttcagcagtg tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga
    30901 atcaccaccg acatctactc caatttgttc ctttaatata cttgaattat ttcttaaagc
    30961 atttaaatgc atttgcatcg tccaaccata tcttgtatac tcagacatca aatgtaattg
    31021 aatacctgta acaaattgtg caatttctga taaatggaaa tccgtttttc ctgctaaagc
    31081 ttttattaac agattgtcga gtattttttc atctgctatt tcataaaaat aagtattcaa
    31141 accgtgatct gctaatcgac agccaatttg atgaaaataa tccactcgtt ggctcaatgc
    31201 ctgcttcaac ccattaaagt ttttgattgg tatacccgct gcttgactaa gttttaaaat
    31261 atagttcgaa aatcctgatg atttgatact ccagacctta tctggacgga aagtaggtaa
    31321 taccttgaaa gaattatcct gtttagaaag ctcagtatga tatcgcaagt catctgtagg
    31381 atcatccgtt gtacaaatgg ctttcacatc catcatcttc agcaattctt ttgggcgata
    31441 ggaatccgtt tggatttttt cgttggcacg tcgccaaatt tctggtgcat tcttctcaga
    31501 aattgtcaaa tcaatatcaa agtaacgttg taattccaaa tgagaccatt caaaaatagg
    31561 attacctggc gccttctcca ctgtttttac aaaagctaaa aatttttcat aatcattacc
    31621 tgcaccactg atcaactctt cttctatccc attagctcgc atcaatcgcc atttataatg
    31681 atctccagct cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc
    31741 tagcaaacat gctgggtcta aatgacaatg ataatcgatg attggttggt gttctgcaaa
    31801 tttatgaaaa agtcttttag ctatttcatt ctctaataaa aagttcttat ctaaaaacat
    31861 atttaagcac tcctttcctt cccttagttt ttagaagaga aaatttttct tctaaataga
    31921 ctccattata taattattta ctcgccccct tttcaagcgc ttcccaaata ccatttaaat
    31981 aaactgcacc tagagctcgg tcatataacc cataaccagg tctaccggtt tctccccaaa
    32041 tcatccgacc atgatctgga cgaatatagc cattaaatcc attatcgtac attgttttta
    32101 atatcttata catatctaga gaaccatatt ctgatttatg cggcgcttca taaaaatcga
    32161 gtccattatc acttaaatat ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt
    32221 gacaaaattc agatagaatt tcatagacat cattatctgg tcgttctgca atacttccag
    32281 tacataacgt aataccatta gaaggagaat ccactacatt acacaaccat tctaaatcgt
    32341 cacgattttt aacaatacga ggtaatccga aaatagaata tggtggatca tctggatgaa
    32401 ctgccatttt aatcccaact tcttcacatg tcgggataat tgctttgaga aaatatgcaa
    32461 agttctctcg caatttcgct tcatcaactt ctttataggc ttctaataat tgcttgattt
    32521 gtgataaccg atgaggttcc caacccggca attcaaaatt attagtagaa ttttctactt
    32581 ttctaataat ttcctctgga tcagtaggaa tattttttga aatataggct aacgtagaag
    32641 acccatcatc taaatgataa tccaaatctg attttaccca atcaaagact ggcataaaat
    32701 tataacagat aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt ttataattct
    32761 cgatgtattg atctcgtgtt ggcaaaccaa ttttgatatc atcatgaaca ttaacactct
    32821 caataacttt caaggtaagg ccattggctt cgacttcatc ttttaaagct ttaattcgtt
    32881 ctctcggcca gacctctccg gctggtatat ccattaatgt tccaacaatt cctttcattg
    32941 ctgggatctg acgaatttct tgtagtgaga ttttttcttc atttgcccca taccatctaa
    33001 aagtcatttc catagttact ttcctccaac ttctgttata tatttattga ttgttttacg
    33061 aactgcttga ggacctacta gcatttcgca aaagattttt tcaattttct ttcctatctc
    33121 aacttgatat aaatctaaac caaatatttc cttattttct aagatttcct gtaccgcaca
    33181 atgaatcttt gcaggatctt gttcacctaa tttcaatact gagaattttt cctgcaactc
    33241 taccaataaa ggatcaggac ttggcacaaa attttttcct tgatcatcga ctgccaataa
    33301 ataacgtaac caagcagcaa tagcgatagg aataaatgtt agattttctg catttccgtc
    33361 tttttcgaca tactttttaa ttgtttcacc atagcgaatc gctacttttt gagaagtatc
    33421 tgttgcaatt cgttgcggtg tatctggaat atagggatta ggcaaacgtt tattgattac
    33481 ttcatcaata aattgttttg gatcaataac gcctggactt tcaactactg gtaagccttc
    33541 tacataacct atccctttaa ttaagccaac caaatcatca tcttgcattt ctgcctgaat
    33601 tgatttatac cccaacaaac aacctgttat tgctaatgcc gtatggagcg gatttaaaca
    33661 tgtcgtaacc ttcatgatat ctgctttatc aactgtttct ctatctgtca taatcacacc
    33721 agccgcttcc agtttaggtc gaccatttgg aaaactatct tcaatgacta gataatggac
    33781 tttttcagta ttggtaaaag cagcgatatt ggtatttttc gcagtatgaa ttatatctaa
    33841 gtcttcgaaa cccgccttca ataaatccat tttgacactt tccgaaggat ttggcgtaat
    33901 tctatcaatc atactccaag gaaaagtcac tttttcttta tcctttaaat aagcaataaa
    33961 tttttcagaa acgtatcctt ttttttgcca agcttctgca atcataaaaa tgctatcttg
    34021 aaaacgttga ccattctgtg agaaattatc agtagaaacc atcgcaatcg gtaactctcc
    34081 agcattaaac ctgctataca acaaggatgt cacaatactc atcgtatgtt gaggattggt
    34141 aggtccagat tttatatctg cttccactac cgaaaaaaat tgtcctttag aatcttttaa
    34201 actgtatcct ttttcagtaa tagtaaacgt tgtaaactgt aatgtaggat cttcaaaata
    34261 tttaaccact ctttgatagt ccgttatatt ctttggattg caatataaac tatcagctgt
    34321 tactgctaaa agtctttttt tgagttcacc atcttcaccc ataacaatct ccaaaatatc
    34381 attgttgtag tcatgataag ctttttctac tacttcttca tcaaatgtct cacacacaac
    34441 tactccggat tctaattcat tttgatctgc taagcattga gcaatttctg catgaaatcc
    34501 tcgataaaga tttcctccac caaaatgaag ccacactgga tgtttctgac cttcttgttt
    34561 gaccgcttcg acattaaaat acggtagttg aacttgcatt ttgttcaatc cttcgataga
    34621 atcttgccag tctttaatca aaaccatatt tacccctcca tactgatatg ttagtttccg
    34681 ttacaattgt agtataaaat cgaatgtaat cgctgtcaat aagaaaaaac taaaaatatt
    34741 cttttttcta ccatgtcaga attattgtgt ctttttttag taaaagcaca ccaatatata
    34801 ttttttagaa ctaacttaaa ttaaagctta atttctcttt gatcttattt gagtattttt
    34861 tgttttttta atagctagta tgttactttg aaatttgata ttatataacg agcatagaac
    34921 taaaatatca aattcaaaaa tgaaattttg agagggatta aatatgacaa ttaaaactag
    34981 tctccaaaat caagcttatc aatctattcg tcagcaaatt atctacgctg acttagaacc
    35041 tggaaaaaaa gtatcagaaa aaaaattgga agaaacgctt tctatcggtc gtactccaat
    35101 acgtgaagca ttgattcaac tccgtcatca aggacttgtt gatacaattc ctcaaagtgg
    35161 aaattatatc tcattaatcg acttagattt agcaaaaaat gctcgttttg tcagagaaca
    35221 tttagaacga caaattatgc tagagtgttg tgccaagatg aataatcaaa agaaacaagt
    35281 tttagaaact ataattgcag aacaagaaaa agctgttagt caaaagaatg ctcgtagttt
    35341 ttttcataat gataatttat ttcataaagc ttgttttgaa attgcagaac gggaagaagt
    35401 atggcaatgg ttggaagata ataatacgca cttggaacga tttcgttggt tacgagtaaa
    35461 aacggaagga ttaaaatggg aaaaaattat ggaacaacat catcaactat ttaaagcctt
    35521 gaccatgcaa gaccctgaag aagctaactt cttaacatcc ttgcatcttc atttaatgct
    35581 tgatgaacaa aatgttgtat tgactactca cccaaaatat tttaaaaata tataactgct
    35641 aaatttcttt tttttaattt atttacattt ctgattacta cagaaaacta actacttttt
    35701 ttatgtaaag ttctgtcaaa tatagtcact ttatattact ttctcttgag taaaaaaaca
    35761 tgtgaggtcg taaacaaaga ttgaactatc tacattcata ctctataatc tgtttatttg
    35821 cagactgata atagcaaaag aaaacttata atcaaataat atcaacattt gatcacataa
    35881 tttgagattg aatgctgatt tacctatgtt ttacgtaatg gtctttagtt atttttgctc
    35941 ataaaatgca taaaacacgc gtttttatgt gtgcttgaaa tttcttggct ctataatttc
    36001 taagactgat aaaaatattc tatcggttct ttttgcaaat aaaaaaacat atagaagctc
    36061 cattagattg aatcaccaca acacaatcaa aggagcattc cgtatgcttg attttcattc
    36121 tataaaacta cttttaggaa tcaaagaaaa tcacaacggt tcaaactaaa agcaagaata
    36181 tccgaagtac cattgtttca tcgatcatga gaaaagcatt cacctctgtc tgcagatgtt
    36241 cttatatcgc tctgtcagtc ctattaataa agtactgttt tgttttcaaa aaaactatta
    36301 cgagtacttt ttaagagaat gccttgcaaa tattcatcac acaccgttat ataagtcaaa
    36361 tagtttcttt cagtcgtcaa gaaaagattc tcctgtcctt ggcattatca gttaagtagc
    36421 aacttaattc tcggacttgt tctactctag caaaatttat tttcatcaac ttacgtccaa
    36481 atgttttttt gaataattac ttcatcgcgg gtctggaaga cttgcctcat tcgataagtc
    36541 actagctttt ggcgtccttc aggatttcta gtatacacca actgacattt gggcagcgac
    36601 gattgattta attcgtttag aaaaaggtta gcgatttgct tatttcggcc aaagcgttct
    36661 ggtaccgtca gaactatctt tttcccttcg attaaatacc gcggattctc cagcggtgct
    36721 aaaagctccc tcatagcttc agtaaaaagc tgtccttctc gatagccagc attctcaaga
    36781 tagcatctaa attcctgctg atcagccgct acgattactt ttctatcact aacttcacca
    36841 aggcgctgca acgtcgtttg aacgcatatg gccaagcttt ttttgcgggc acattccttt
    36901 tcccatagct tttgtcgcct acgaatttgg aaatattcca gcaagttacc tttaaatata
    36961 gctgaccaga agcttagagg ctctatattt tttactacta tcggcaatga agtttcttgt
    37021 ttttgcttta cctgatcgac tttcctaacg acgattggca tagataggtg ccagccatca
    37081 gctaaagcag tactccattg ttctttcaac cgacttcgtt cacaagaaaa atagaacatc
    37141 gactgagtat aagtttccat cgtggtggtc gtatattcca gtggtaaagc aaagcgatcg
    37201 ccaccactct caatttttgg tgaagattca aaggtcagac ctgaaatatt gcccaaacgc
    37261 cgtagcattc ttttcaaatc aggctgttca gacagaggaa ctcccggtaa tccaatcgct
    37321 aaatgccaaa tgttcgttac tttattttgt gcacctaggc gtaagccccg ccctcgtagt
    37381 tgttgtgttt gcacaaaagt ggaagcttga ttagccaaga tcaacagatt aattgcagga
    37441 caatcccagc cttctcctag caaactaacg gttcctacca aaatattcaa cttcttttcg
    37501 gttaataggc gagtaactgc ttgcatcaat gcttggcggt ttttgtcatc caccgtcaaa
    37561 taaaaataac ccgacatata aggatgttca ttcatacccg tcgctagttc tgccagctca
    37621 acagttacta gcattttcgg taacagtaag aactctccac agatggcacc gatttgctga
    37681 gtgttaccta atagacgaac aacttcatca aacaaaggaa ccacgccata agctaaggta
    37741 ccatcactgc ctgctaatac ctccttgcaa atacgatcca tcaaaatgac cgctgtcagt
    37801 tcttgcttca gctgaacttc ttcttttaac aaaatttgac aaactgcttg ctttttctca
    37861 ggaatcgcct gactaccaac agtgtctgga tactctggaa acaaatgcag cgactcatca
    37921 aacaatcatc cagctttttt caattgataa aaaataggca attccggttg atcttcaaac
    37981 atctgctgat aaatagtgat cagctctttt ttacctaaag atggaactga agctttttta
    38041 ccttgctgct tagaactgcc taatagctgc caatgttcgg gtatcaattc atagttatga
    38101 tgattcaagt aaattaatgc actagtgaac agctcaaaat tttcataaat ttccttcgaa
    38161 tgctcaagcg gatgctgaac aaattctaaa gaaagcaaat aatccaccgt ctcttggctt
    38221 tccattaacg cttccaccag ctcattttgt ttctctaaaa aggtaaaata atgctgagta
    38281 gtttcctgat tagccggaat caaatataga aaatcctgat gaggacatag aacctcctcc
    38341 tttaccaact cgggaatgct aatttcttcg tcaatcaggc cattttaact caatatattg
    38401 cttccactcc gtctgagagc tatcaatcgg aggcgtggct gtcaaggaaa tcgtcaacag
    38461 attcggttgt tttttcttca agtcaatcaa caattcaccc cagctttttt ttaaatgatg
    38521 agcttcatca aaaaactaac agctccagct gatcaccata agttggctcc tccttcagtc
    38581 ggttgtaaat ggtctgatac gtttccactg taatatgact aggatgttca atcgccccag
    38641 aaagatagca actatctgct ggatttacta agaaatattc ctcgattgtt gttaaccact
    38701 gtttcttcaa tattagggaa ggaaccaaaa ttaaggtttt acaatccaaa cgtcggatca
    38761 tctcaattcc taaaaaggtc ttgccagccc ctggtggagc cactagatgg actgaattgt
    38821 tatttaaata agtagcaaag ttagtcaata tacgcttctg atagctacgc cactgaattt
    38881 tttcatcaga tagccacatc ccaggtcccc ctctcacgta ttttctctat cataatctaa
    38941 atgaatacag caaaaaaaag atttcttcaa gaatatgtgt ttttatagta atattttact
    39001 tactctaaac tcaaatgaaa aggtaaaaaa actggtagta caatctttta aattctcatt
    39061 gttttgtacg ttctttacta tcaaaaaaac aaaaaaaacg cgtttctacg cgtgaccaaa
    39121 atttttagct ctataatttc taagactgat agaaaatcat tctgtttttg aagtcttttt
    39181 tcataaatac tacttgattt attgtgtata ttgtataaaa gattgttcat aattattttt
    39241 tataattaaa atttggtatt gatgaccagc gattccgtat ataatgcgca atcaaattaa
    39301 gctttctgtc tctggtaaat attcctgttc tgttttcttg tactcgtaag atttcttcac
    39361 ttatcgcaaa attcataaaa ttccacactt tttcataaac aaaatgtaca catttatcta
    39421 ttacttcata atagtctact tgatattctt ctgtaaacat ggttgctact gtatctgcac
    39481 catatttgat aaacatttgg gctactccct taaattgaga aaagcccatt agtttatcta
    39541 ctaatacttc taaaatttct tgctttcttc tttaacatcg tcccgtaagg aatcatgtag
    39601 tatgctggag gaagttcatt ttctaaataa ttgtatcgct tcaattgctc aaaggctatt
    39661 ctctacaatt aattacacca attactttat cttcacttat ataagatttc taaacatttt
    39721 tcgtcaaatg aagagaaaat atttttttaa atgatcttgc tcaagatttg tcaacgaatc
    39781 tttgaattcg ttttgaagtt ctgaggaagg tatcatttaa aaactcaatc tgactttcat
    39841 catttcgttt actcccattt tgatcacctt ctaccaacaa tttattaaag tgattatact
    39901 aggtttttat ttttttaatt cacaattgtt tattcacttc atgaacaatt aaacacaaaa
    39961 tcagtattag gattaaactt tcacccatta atacactaac gaaaaaattt tcatttttta
    40021 aaatagctaa actataaatc tctatactga aaaaaacaat acccatcaaa aata
//
LOCUS       DACDTH010000020        39224 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDTH010000020 DACDTH010000000
VERSION     DACDTH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.020"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IWK54_001107"
     CDS             131..505
                     /locus_tag="IWK54_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9593244.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IWK54_001108"
     CDS             complement(619..1248)
                     /locus_tag="IWK54_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9593245.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IWK54_001109"
     tRNA            complement(1382..1454)
                     /locus_tag="IWK54_001109"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IWK54_001110"
     tRNA            1668..1741
                     /locus_tag="IWK54_001110"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IWK54_001111"
     CDS             1696..1845
                     /locus_tag="IWK54_001111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593246.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IWK54_001112"
     CDS             2338..3666
                     /locus_tag="IWK54_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9593247.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IWK54_001113"
     CDS             3868..4701
                     /locus_tag="IWK54_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9593248.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IWK54_001114"
     CDS             4717..5454
                     /locus_tag="IWK54_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9593249.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6535)
                     /locus_tag="IWK54_001115"
     CDS             complement(5609..6535)
                     /locus_tag="IWK54_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9593250.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6810..7643
                     /locus_tag="IWK54_001116"
     CDS             6810..7643
                     /locus_tag="IWK54_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9593251.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7677..8516
                     /locus_tag="IWK54_001117"
     CDS             7677..8516
                     /locus_tag="IWK54_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9593252.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8542..9102
                     /locus_tag="IWK54_001118"
     CDS             8542..9102
                     /locus_tag="IWK54_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9593253.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9301..11022
                     /locus_tag="IWK54_001119"
     CDS             9301..11022
                     /locus_tag="IWK54_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9593254.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11187..12329
                     /locus_tag="IWK54_001120"
     CDS             11187..12329
                     /locus_tag="IWK54_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9593255.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12345..13556
                     /gene="thiI"
                     /locus_tag="IWK54_001121"
     CDS             12345..13556
                     /gene="thiI"
                     /locus_tag="IWK54_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9593256.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14129..14776
                     /locus_tag="IWK54_001122"
     CDS             14129..14776
                     /locus_tag="IWK54_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9593257.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15169..17814
                     /locus_tag="IWK54_001123"
     CDS             15169..17814
                     /locus_tag="IWK54_001123"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9593258.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18124..19437
                     /locus_tag="IWK54_001124"
     CDS             18124..19437
                     /locus_tag="IWK54_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9593259.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19427..20080
                     /locus_tag="IWK54_001125"
     CDS             19427..20080
                     /locus_tag="IWK54_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9593260.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20135..20686
                     /gene="radC"
                     /locus_tag="IWK54_001126"
                     /pseudo
     CDS             20135..20686
                     /gene="radC"
                     /locus_tag="IWK54_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            20817..21902
                     /locus_tag="IWK54_001127"
     CDS             20817..21902
                     /locus_tag="IWK54_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9593261.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            21899..23791
                     /locus_tag="IWK54_001128"
     CDS             21899..23791
                     /locus_tag="IWK54_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9593262.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            23798..24175
                     /locus_tag="IWK54_001129"
     CDS             23798..24175
                     /locus_tag="IWK54_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9593263.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            24329..25111
                     /locus_tag="IWK54_001130"
     CDS             24329..25111
                     /locus_tag="IWK54_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP9593264.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(25237..25968)
                     /gene="erm(A)"
                     /locus_tag="IWK54_001131"
     CDS             complement(25237..25968)
                     /gene="erm(A)"
                     /locus_tag="IWK54_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP9593265.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(26026..26113)
                     /locus_tag="IWK54_001132"
                     /pseudo
     CDS             complement(26026..26113)
                     /locus_tag="IWK54_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            26477..27139
                     /locus_tag="IWK54_001133"
     CDS             26477..27139
                     /locus_tag="IWK54_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9593266.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            27374..27517
                     /locus_tag="IWK54_001134"
                     /pseudo
     CDS             27374..27517
                     /locus_tag="IWK54_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            27667..27867
                     /locus_tag="IWK54_001135"
     CDS             27667..27867
                     /locus_tag="IWK54_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9593267.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(28121..29800)
                     /locus_tag="IWK54_001136"
     CDS             complement(28121..29800)
                     /locus_tag="IWK54_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9593268.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(29802..30014)
                     /locus_tag="IWK54_001137"
     CDS             complement(29802..30014)
                     /locus_tag="IWK54_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9593269.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            30407..32137
                     /gene="efrA"
                     /locus_tag="IWK54_001138"
     CDS             30407..32137
                     /gene="efrA"
                     /locus_tag="IWK54_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9593270.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            32134..33894
                     /gene="efrB"
                     /locus_tag="IWK54_001139"
     CDS             32134..33894
                     /gene="efrB"
                     /locus_tag="IWK54_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9593271.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            33989..34186
                     /locus_tag="IWK54_001140"
     CDS             33989..34186
                     /locus_tag="IWK54_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593272.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            34339..34824
                     /locus_tag="IWK54_001141"
     CDS             34339..34824
                     /locus_tag="IWK54_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9593273.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(34928..35521)
                     /gene="clpP"
                     /locus_tag="IWK54_001142"
     CDS             complement(34928..35521)
                     /gene="clpP"
                     /locus_tag="IWK54_001142"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9593274.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            35701..36228
                     /locus_tag="IWK54_001143"
     CDS             35701..36228
                     /locus_tag="IWK54_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9593275.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            36319..37056
                     /locus_tag="IWK54_001144"
     CDS             36319..37056
                     /locus_tag="IWK54_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9593276.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            37060..37977
                     /gene="miaA"
                     /locus_tag="IWK54_001145"
     CDS             37060..37977
                     /gene="miaA"
                     /locus_tag="IWK54_001145"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9593277.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            37979..39208
                     /gene="hflX"
                     /locus_tag="IWK54_001146"
     CDS             37979..39208
                     /gene="hflX"
                     /locus_tag="IWK54_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9593278.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        13178 a         6425 c         8149 g        11472 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt tttttcattt tacagacttc cttacgaata
     6601 attttgcata gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa
     6661 gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc
     6721 aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc
     6781 aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag
     6841 aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac
     6901 ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt
     6961 ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg
     7021 gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag
     7081 atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct
     7141 catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg
     7201 agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg
     7261 aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac
     7321 agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt
     7381 ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa
     7441 aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag
     7501 tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg
     7561 cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc
     7621 caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga
     7681 tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg
     7741 aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa
     7801 cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg
     7861 tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg
     7921 aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt
     7981 ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta
     8041 tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc
     8101 tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag
     8161 aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc
     8221 gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag
     8281 gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag
     8341 aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg
     8401 gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat
     8461 caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa
     8521 tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc
     8581 cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct
     8641 catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct
     8701 aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa
     8761 gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca
     8821 ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat
     8881 cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct
     8941 cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct
     9001 ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag
     9061 aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt
     9121 tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa
     9181 atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta
     9241 tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa
     9301 atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc
     9361 tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa
     9421 cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg
     9481 catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata
     9541 tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa
     9601 agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa
     9661 atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa
     9721 cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc
     9781 agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg
     9841 atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc
     9901 cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa
     9961 attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt
    10021 gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa
    10081 gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa
    10141 gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat
    10201 atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca
    10261 gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca
    10321 tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa
    10381 gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt
    10441 ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa
    10501 aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg
    10561 caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa
    10621 caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt
    10681 attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt
    10741 ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta
    10801 gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac
    10861 gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat
    10921 caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa
    10981 cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa agttagtaaa
    11041 ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata tttgaaaatt
    11101 gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt ataatagaaa
    11161 agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca acaaccccaa
    11221 tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt gggaatccat
    11281 caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca agaaagcaga
    11341 tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc ggaacagaag
    11401 gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg aatcatatga
    11461 tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta aaagaattag
    11521 gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc gacgaattga
    11581 aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat aatgaagtag
    11641 gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct aaaattcatt
    11701 tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg ttgacagaaa
    11761 gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga actggtttca
    11821 tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg caagaaagaa
    11881 atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa gctttacgtt
    11941 tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa gtagctctaa
    12001 tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact gactatgcac
    12061 cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc catgccttag
    12121 aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa catatggcaa
    12181 gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct gttcgaatca
    12241 gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt ttcaagcagc
    12301 tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa tacagtgaaa
    12361 ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc tttatcatgc
    12421 aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa atccatgctg
    12481 atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt attccaaaac
    12541 tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa aaagatgtcg
    12601 aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg aaagaaacat
    12661 ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat gaattgaatc
    12721 aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa atgcgtcaac
    12781 cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg tatgaaacga
    12841 tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt atgctttctg
    12901 gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg gaaattgaag
    12961 ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa gcaaaagatc
    13021 taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg ccatttaccg
    13081 agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca attactcgtc
    13141 gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta gtcattttga
    13201 atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg gctatcaatg
    13261 aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt gaaatcattg
    13321 aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa gattgctgta
    13381 cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc catcaatatg
    13441 aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg aagatggaag
    13501 agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg ctataagagg
    13561 ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa tagctccatg
    13621 tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat ttatcaagaa
    13681 gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt tagagatcaa
    13741 gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga ggttgtgaca
    13801 gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc aaatttttgt
    13861 gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc agaggttgcg
    13921 aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt
    13981 acgtttggtt gagcttatta ccgcagatgc aagcttttga acaccgtttt atttgtctca
    14041 cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt acactaaata
    14101 tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac tgccaaaagc
    14161 aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg actcaggtat
    14221 aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg caacgatccg
    14281 cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg atgtcccttt
    14341 tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa tcgcattagt
    14401 aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa atgaaaatct
    14461 aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa ttgatggaga
    14521 aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg tgacagtagc
    14581 aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg tcaaaggaaa
    14641 tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt atgtcaatgt
    14701 tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg agaattactc
    14761 agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa ctcgtcgtaa
    14821 ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac ttgttgacta
    14881 aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa ctggcacatt
    14941 cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt gaaggtagct
    15001 tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta actcacttga
    15061 gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga
    15121 atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat gacggaagaa
    15181 aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta tcagcattgg
    15241 ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta ttcaatcgta
    15301 atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga tacgaccttg
    15361 caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg gctgcctggt
    15421 atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag agagcaaggg
    15481 atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa
    15541 gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt agattacagt
    15601 agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt ttttgttacc
    15661 ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga tcctcaagca
    15721 cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc cttttatcat
    15781 atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa
    15841 acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta tcaagcgctc
    15901 atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat tgcagatgaa
    15961 tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca tgatccaaac
    16021 gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga tgatgatggt
    16081 acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc tcgtaaagca
    16141 atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat gatccacagt
    16201 gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt
    16261 gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga tcaagcagtg
    16321 gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa tgtccatgat
    16381 tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta tcacaaagaa
    16441 acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg ggtacaagat
    16501 cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac aatgggctgg
    16561 ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt agtaaccggt
    16621 tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga atttactggc
    16681 gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca agggcggaag
    16741 atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa gtatggagca
    16801 gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt ccgcttcagc
    16861 tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc
    16921 gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg agaaaaatca
    16981 gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt aactgagcta
    17041 tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat ctgggatgat
    17101 ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga tgctgtttca
    17161 aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg tttattgcat
    17221 ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca gggaaattct
    17281 ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac agcttctaaa
    17341 gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc agaagtgaat
    17401 acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa aatcgatcaa
    17461 tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga gttgacgata
    17521 tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg tgcagaaatc
    17581 ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa
    17641 ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt
    17701 gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa agattattta
    17761 gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca ataagaatag
    17821 aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga acatcgttac
    17881 aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga cataaatttt
    17941 gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg tggatcatgt
    18001 atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt ttctctttgt
    18061 catactttta tttttcttat gtgttctatt tgttataata aaagagaaat gggggaattg
    18121 ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt tggttcacgt
    18181 cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga gagaaaagta
    18241 aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta tttgagaagc
    18301 ctattagaag aggctgggct gacagtaggt acatttactt caccttatat cgaaacgttc
    18361 aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat ccagcttgta
    18421 aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc tggtattact
    18481 gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa agtggacgta
    18541 gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc tgtaccaatg
    18601 ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga tacaattgaa
    18661 gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt aacaggaaat
    18721 attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt
    18781 tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat gtggggagaa
    18841 gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc gcttttaggg
    18901 caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta ctgtcaattg
    18961 gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca
    19021 ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc ccataatacg
    19081 catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac
    19141 attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg attgcagcaa
    19201 gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat cgaattaagt
    19261 gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca attcggatta
    19321 gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg ttcactttat
    19381 ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga agtataatgc
    19441 agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt acgaagccac
    19501 acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt taaaattctt
    19561 aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg aatttggcaa
    19621 aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac gtttttcgca
    19681 aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag aagaacatca
    19741 gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt tattgaagaa
    19801 gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa
    19861 accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga agaaagaaac
    19921 attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg agatcccggt
    19981 agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa cagctggggt
    20041 tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct cttttcgtat
    20101 cttattttag ataagataga aaggagatgt ttttttggga aaacgattga tcaaagaagt
    20161 acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag ctttatcgga
    20221 tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg tgatgtcgat
    20281 tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg ctactttgca
    20341 tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca aagccttgat
    20401 cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg ttcgtaccag
    20461 ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag aacatttggt
    20521 ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc
    20581 gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac gttattgtgc
    20641 agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca tagtataata
    20701 aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta ctttaaataa
    20761 aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg gaacatgtga
    20821 aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta ctagataaag
    20881 aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat actggtaagt
    20941 ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag ttcatggaac
    21001 agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc gtaggttggt
    21061 tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc ataagagaag
    21121 aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat ttaagtagat
    21181 taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat ttcaaaggat
    21241 ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta agggttaaaa
    21301 agaaacagat aagaacattg agatcagagg aagttaagca aattattgat gcttgtcata
    21361 cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga atcggtgaag
    21421 tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat ttaacaccta
    21481 gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca atacatgtga
    21541 gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt gaggaattgg
    21601 agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca ctaaagtacc
    21661 aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa tttacatcac
    21721 atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat gttgcgttcg
    21781 ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat gttcatcttt
    21841 cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag cataagaaat
    21901 gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa aaatagctgt
    21961 aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa gaaaatgtcc
    22021 acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt gggttcgttt
    22081 tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt tttaccattt
    22141 aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag taattaataa
    22201 aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg aagttcctat
    22261 tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta gaattaccac
    22321 tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta attcctacta
    22381 tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg atggagagga
    22441 gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata aggttaaccc
    22501 aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt ttaaacaact
    22561 tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg taagtgatat
    22621 tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac aagttcagag
    22681 agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa acatgatggg
    22741 aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat ttagtaccct
    22801 tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg aggactatcc
    22861 gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc aattgaacag
    22921 tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc aagaatgtgg
    22981 aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg acaaagatgg
    23041 agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag ttccaatatc
    23101 taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg aatttccaga
    23161 tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa catttagagg
    23221 tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg agatttatag
    23281 attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca acgggatgcc
    23341 ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa gcagatacgc
    23401 tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa aactggttac
    23461 caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg tagagcttca
    23521 atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta gattgccagt
    23581 agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact tctgtaccag
    23641 taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt tattagccat
    23701 agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta aagagcgttt
    23761 agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag ttagaaatac
    23821 aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa aagtagacaa
    23881 agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa tagcaaagga
    23941 agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa gaatcgaatc
    24001 ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa gttctcgttc
    24061 ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa aagaaaataa
    24121 aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag aataattatt
    24181 aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct ttattgaaca
    24241 gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat aaactttata
    24301 tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa aaataccaaa
    24361 tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa tagttggagt
    24421 atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg tagatgttct
    24481 agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag aaagactaat
    24541 gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag ttacggttat
    24601 aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta tatacggtga
    24661 gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg atcctgattt
    24721 ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc ctgattcttc
    24781 aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg attctttgcc
    24841 agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc tagctcgaat
    24901 gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag aatgggctat
    24961 acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag gctatcgggg
    25021 agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg ttaagtatat
    25081 gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa caggtgctta
    25141 cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga ataaagcgtc
    25201 ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt tgtaactatt
    25261 gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat ttgcatgctt
    25321 caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt ttacccactt
    25381 ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt gatgtcgttc
    25441 aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg gtggtacttt
    25501 tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc gttgcagatt
    25561 ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag cctgactttc
    25621 aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac catatatctt
    25681 atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca cttttatatt
    25741 ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa tttctatagc
    25801 agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc ctgatccgat
    25861 ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt ttacatgctt
    25921 tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta taaccttctc
    25981 cttactggtt ataatgaact gacattaagc gttcattata accaattaat tttgatttgg
    26041 ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt tatctccttt
    26101 ccattttttt cattatgaat gagataaagt aatttctact actgcgatac tggtgcacat
    26161 aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt tagaataaac
    26221 ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa ataatatgtt
    26281 ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc gataaaggac
    26341 agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc taaagggaag
    26401 ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt cgataaagta
    26461 ggaatggggg atctttttga atacaataga atcacagttt gataaggttg cagaagatta
    26521 cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata tgtctccaaa
    26581 gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga aattagcaag
    26641 ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc ttgccaaatc
    26701 taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac ttaattttaa
    26761 tgagaagttt gattttattg taagccgaac aacctttcat catttggatg atatagccag
    26821 cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta ttcttgataa
    26881 tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa tccaagagtt
    26941 tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata atcacaacac
    27001 atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac aaaattatta
    27061 cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt gggcaatggg
    27121 ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt gtgattgttt
    27181 tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc ctgtatctta
    27241 tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag taccttccac
    27301 ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg taatttactc
    27361 ttgataatat gaaattccat cgcagcagga tatgaatttt accaaaagga tacaaaagtg
    27421 tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca aacgatattt
    27481 tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg
    27541 aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa catctgttgc
    27601 acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca cgaggaggat
    27661 attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc
    27721 tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg tgacggattc
    27781 aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga ccgcggccca
    27841 caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat ggtagacatc
    27901 aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga caatttctct
    27961 tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat gaaagagcca
    28021 ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca tttgttttct
    28081 cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca tctggtgtca
    28141 ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg
    28201 cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc aaacattctc
    28261 ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat tctcgcatgt
    28321 agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc ttgatatcca
    28381 ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca cgtaaaacga
    28441 cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc acaaaatgcc
    28501 cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc atgatgaagc
    28561 agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc aacatgcgga
    28621 attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt aatttttgtt
    28681 cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga atcacttctg
    28741 caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta ccaggaatag
    28801 gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg tgagtaccat
    28861 tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat aggatcatcg
    28921 tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg tctggaactt
    28981 tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt ccgaaaacag
    29041 cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg aagatgttag
    29101 aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc aaaatagaat
    29161 gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta ctatcagata
    29221 aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt gctggttcgc
    29281 caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca gaaggtgttt
    29341 tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag ctgacagaag
    29401 tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca tctcgaagca
    29461 agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca gcatagatag
    29521 gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg tgcccgtgag
    29581 taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga
    29641 catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca atcaaaatga
    29701 tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat ccaccaatag
    29761 caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc cactaatgag
    29821 aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg aacgaattca
    29881 atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct ttttgcctca
    29941 atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc ttgataataa
    30001 actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt agctagttga
    30061 tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat gctttgacta
    30121 gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt cggatatttt
    30181 ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct agcataaaac
    30241 agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta tcatcccgct
    30301 ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt tgtgctatca
    30361 tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga aactaatgtg
    30421 gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg tatttggttt
    30481 tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg taggaatccg
    30541 caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta ttactgtaat
    30601 cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga ctaatatcgt
    30661 agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc aggaatacga
    30721 aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc agatcatgtt
    30781 gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca ttgtcagttt
    30841 gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg cattaccttt
    30901 actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca
    30961 aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat
    31021 tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca atgagaatta
    31081 tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg gtttttattt
    31141 cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg
    31201 cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg ccttgttctc
    31261 attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt cagctggacg
    31321 tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg gtatatctga
    31381 tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc caggccattc
    31441 gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga ccgttgcttt
    31501 tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc gcttttatga
    31561 tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc ggctaagcaa
    31621 gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg gaacgattgc
    31681 agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac gtgcagctga
    31741 gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg aacatttatc
    31801 agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag ccagagccat
    31861 catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag attatcaaac
    31921 ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg tattgatcgt
    31981 agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa atgaaggaga
    32041 agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt attatgatat
    32101 tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa
    32161 cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat cctatttaca
    32221 gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc tactacagaa
    32281 atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt caaatgtctc
    32341 atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc tggtgttttg
    32401 atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga agaaaaaatc
    32461 aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg
    32521 acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg tatcgtaaat
    32581 gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc gatgatggcg
    32641 ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt gatcaatgct
    32701 tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg ctacgtccaa
    32761 gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga aacattggaa
    32821 ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc attcatttct
    32881 ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg tatggctgtt
    32941 ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca agcattcatc
    33001 caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc ttcagttttg
    33061 caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa
    33121 gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac attcgatcat
    33181 gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt tgaagtgaaa
    33241 gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac attgatcaat
    33301 ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg aatcgacacc
    33361 aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca agatgcatgg
    33421 ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc gacagattat
    33481 gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac gatgccagat
    33541 ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca aaaacagttg
    33601 ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga tgaagcaaca
    33661 agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg cgtgatggag
    33721 ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt
    33781 ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt attggcacaa
    33841 ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga ataagtcata
    33901 taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat ggtaaagtaa
    33961 agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa aaattactga
    34021 agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt ttgattcagc
    34081 tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag aagtgtacga
    34141 attaatttta aaaaaagtaa tagaagaaaa tatagatgta caataaaaac aggtaaaacg
    34201 tcagttacca gatactgtaa atagacatct ggcctgagca ggttttacct gttttttttc
    34261 ttatgaaata aatatatgct atgatacctt tattcaatag gattagtttc tacaaaaaat
    34321 gaaaggagaa aaataaatat ggaacggttt atagcatcaa atgccacagt gattggtgat
    34381 gtgacattgt cagaagatgt gacgatctgg tatcaagctg ttttacgcgg ggacagtaat
    34441 tggataaaaa taggacaaag aacgaatatc caagatggta caatcatcca tgtcgatcat
    34501 gatgcacctg tagatattgc ggagaatgtg acggtaggac atcagtgcat gctacatggc
    34561 tgtacaatcg aaaaaggggc gctgatcggg atggggacca cgatattgaa tcatgcggtt
    34621 attggtgaga atagtttgat tggtgctgga tcattagtaa cagaaggaaa agttattcct
    34681 cctaatgtct tggcttttgg tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa
    34741 atacaaaaaa acaaagaaaa catccagcat tatgtcgaga taggtcggat gcatgcgcaa
    34801 ggaaaattca aggagcaaag ataggcagta gcatccataa aataaaaaag gatggtcagt
    34861 ttgagttgac catccttttt tatttggttt agataagaga gcttaaaaaa gcttcctttg
    34921 aaacagatta atttaatgaa gaactgtttt ccatgacttc atcgatcaat ccatatgctt
    34981 ttgcttgttc agctgtcata tagttgtcac gatctgtatc tttttcaatc acttcaagcg
    35041 gttgtccagt gcgttcagct aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc
    35101 gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc gccaagaggt tgatggatca
    35161 tgatctcagc atttggtaaa gcaaatcgtt taccttttgt accagctgtc agcaagaagc
    35221 ttcccattga agcagccata cccataacaa ttgtttggac atctgctttg acaaagttca
    35281 ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc aggagaattg atataaagat
    35341 agatatcttt ttcagaatct tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag
    35401 caagatcatc tgttacttgg ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt
    35461 agatatcgta agctctttca cctcgtgaag attgttcaat gactgtagga attaaattca
    35521 taatagagtt cctccttgat ttcgtttaga aatagtgtac cataatttag tttctaatac
    35581 ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag
    35641 tcgcataaac ccgctttatg gtatgataag cagagaaaca aaacaaggag gaacaacaga
    35701 ttgatagttg gtattactgg ccttatcgga gtgctgacag gtgctgtagt tgtctatatc
    35761 ggcatgatac tgcaaatgcg tcacttggaa aataaagaac agcgtagacg tgaattggaa
    35821 ttgaaagtca aagaaatcga gacgctgaat caattaaata aaaagatcaa cgagatcttg
    35881 caaaaacgga caacgctgat ggaagaatat gtcagctttg atgcatttga tgattgttat
    35941 atcacgattg acgattttgc gtatctccaa tcattttcag cgcaaaatag tttttatctg
    36001 ccaaattata ttttagacga gctatttaag aatatctccc atcgaaaagt gattttatct
    36061 ccagatgaaa caatgaagat tggtggatat gcttataaag gcggacgcgt gattttagag
    36121 aattttacgg atagtctgac tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg
    36181 acgcgccagc cgattggttt cttctcgaaa gatgatgttt ggagctgata ttttgaagca
    36241 ttgccgttgc acatgagagc gtggtaatgc tttctttttt tcttaatgat acaatagaac
    36301 tgaacggagt gaagaaagat gttacaaatc tatgcccatc gcggaagcag cggcacccac
    36361 ccagaaaata cgcttcctgc atttgcagaa gctgtacgag taggtgcaga tggtatcgaa
    36421 ctagatgtcc atctaagcaa agatggttat ttgatcgtta tgcacgacga agaagtggac
    36481 cggacgacta acggtaaagg gctgattcga gaaaaaacat tggaagaact caaaaaattg
    36541 aatgcagggg gcaaattcag caaagaattt ccagcagcta aagttcctga attgagagaa
    36601 gtgttaggac tgctcctcca aaaaaattac cgagggatgc taatgattga aattaaaact
    36661 gatcagtatg aatatcccgg tatcgaagaa aaattagccg aaacattaaa aagtagagat
    36721 tggccttttc aacatgcata ttgcagcttt catttggatt ctttggaaaa aatcgcatta
    36781 ctggaaccgg agatacagct ggacttattg atgagtactt ctgataaaaa gatccaacta
    36841 gcagcagaaa ccccttatat agaaggagta catccaaaaa atacttggtt gttcgaacat
    36901 ccagtccagg cagctgattt tccaaaagca attcgtgcat ggacgattaa cgatgaagaa
    36961 gaaatggcca aatgtcaaga actcaaaata gatggaataa taactgattt tccggagaaa
    37021 gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata tggaaaaagt attagtgatc
    37081 gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg aactcgcaca actatttgga
    37141 ggagagatca tcagtggtga ttcattgcaa atctataaaa atctggatat cggtacagca
    37201 aaaatcaaag aagaagagat gaataatgtt ccgcatcatt tgattgatgt gatcgaacct
    37261 actcaacgtt actcagccgc tgattttcag cattcaggtc gacaattgat taccgagatt
    37321 gcgaaccgag ggcacctacc tatcatagct ggtggtacgg gtctctatat ccaatccctt
    37381 ctatatgatt atcagttagg agcgacagaa gaagcgaatc ctgagattag aaaaaagtat
    37441 gaaacgttag ctgaaacaat tggttcccaa gcattatgga atcagttgaa agaaaaagac
    37501 ccggcagctg ctgagaaaat ccactggaac aatcaacgca aagtgataag agctctggaa
    37561 gttttagaga cgacaggaca tagtatcacg gcacctaaag aagagccaaa gcaattgtat
    37621 gattacctca tgattggatt agatacgaag cgtagtattc tttatgaacg aatcaatcaa
    37681 agagtcgatt tgatgctagc agaaggttta gtagcagaag cgcgtaaggt gtatgagctt
    37741 ggagaagtcc aggccagtca aggtattgga tataaagaat ttttccctta ttttgaaggg
    37801 aaagaaacat tggaagaagc aacagaacaa gtcaaactac attcgagaag atacgcgaaa
    37861 cgtcagttga cgtggtttcg taaccgtttg gatgcacagt ggtttgatct tataagtgag
    37921 ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat
    37981 ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca aaccagcgtt acttttcaga
    38041 gtcaatggaa gagctagttc agctgacgaa taccgcatca ggagaagtcg tattcacaat
    38101 tacacaaaag agaccacaag tggatcggca aacgattatc ggtaaaggga aattacaaga
    38161 attagttcaa caagcggatg cgcatgaagc ggatttgatc atctttaatc atgagctaac
    38221 tccgagacag agccaactga ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca
    38281 gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga
    38341 acttgctcag ttagaatacc tgctgcctcg tttagtaggg caagggaaaa cattatctcg
    38401 tcttggcggt gggatcggaa caagagggcc aggtgaaaca aagttagaaa cagaccgccg
    38461 acatatacgg aataaaattt ttgctgttaa gaaagaattg aaagaagtag aagctcatcg
    38521 agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt cagattggac ttattggcta
    38581 cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca caagcggata cttattcgaa
    38641 agatcaattg tttgcaacgt tagatccatt gactaagaga tggcgttttg ctgaaggatt
    38701 tgagattact gtaacggata ccgtcgggtt catccaagac ctgcctacgc aactgatcga
    38761 tgctttccat tctacattag aagaaagtca aaatatggat cttttgctac atgtagtcga
    38821 tgctagttct ccagatcgta ttttacagga gcagactgtc ttaaaattga tggatgaact
    38881 gaatatgaaa gaaatgccga tactgactgt ttacaataag gcagaccaga tcgacccagc
    38941 gatgttcacg cctacgctat tcccaaatgt attgatttct actcagacga aagaaggtaa
    39001 gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa ctaatgaccc cttatacact
    39061 ttctgtgcca agcaacgaag gacagaaatt aagcgagttg agaagacaaa caatggtttt
    39121 ggacgaagcg tacatggaag aaacaaacga atatgaagtt aaagggtttg caaaaaccaa
    39181 ttcaaaatgg ttaagaaaaa accaataaaa ttttattttt ttaa
//
LOCUS       DACDTH010000021        38840 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDTH010000021 DACDTH010000000
VERSION     DACDTH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.021"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            24..647
                     /locus_tag="IWK54_001147"
     CDS             24..647
                     /locus_tag="IWK54_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593279.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1097..2146
                     /locus_tag="IWK54_001148"
     CDS             1097..2146
                     /locus_tag="IWK54_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9593280.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2143..3024
                     /locus_tag="IWK54_001149"
     CDS             2143..3024
                     /locus_tag="IWK54_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9593281.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3027..3797
                     /locus_tag="IWK54_001150"
     CDS             3027..3797
                     /locus_tag="IWK54_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9593282.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4076..4930
                     /gene="aroE"
                     /locus_tag="IWK54_001151"
     CDS             4076..4930
                     /gene="aroE"
                     /locus_tag="IWK54_001151"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9593283.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5108..6271
                     /locus_tag="IWK54_001152"
     CDS             5108..6271
                     /locus_tag="IWK54_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9593284.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6388..6798
                     /locus_tag="IWK54_001153"
     CDS             6388..6798
                     /locus_tag="IWK54_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9593285.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6972..9200
                     /gene="topB"
                     /locus_tag="IWK54_001154"
     CDS             6972..9200
                     /gene="topB"
                     /locus_tag="IWK54_001154"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9593286.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9338..9655
                     /locus_tag="IWK54_001155"
     CDS             9338..9655
                     /locus_tag="IWK54_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593287.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9815..10051
                     /locus_tag="IWK54_001156"
     CDS             9815..10051
                     /locus_tag="IWK54_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9593288.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10048..10446
                     /locus_tag="IWK54_001157"
     CDS             10048..10446
                     /locus_tag="IWK54_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9593289.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11113..12057)
                     /locus_tag="IWK54_001158"
     CDS             complement(11113..12057)
                     /locus_tag="IWK54_001158"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9593290.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12353..13297)
                     /locus_tag="IWK54_001159"
     CDS             complement(12353..13297)
                     /locus_tag="IWK54_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9593291.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13518..14732
                     /locus_tag="IWK54_001160"
     CDS             13518..14732
                     /locus_tag="IWK54_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9593292.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14754..16769
                     /locus_tag="IWK54_001161"
     CDS             14754..16769
                     /locus_tag="IWK54_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP9593293.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16852..18225)
                     /locus_tag="IWK54_001162"
     CDS             complement(16852..18225)
                     /locus_tag="IWK54_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9593294.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18251..18535)
                     /locus_tag="IWK54_001163"
     CDS             complement(18251..18535)
                     /locus_tag="IWK54_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9593295.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18537..20552)
                     /locus_tag="IWK54_001164"
     CDS             complement(18537..20552)
                     /locus_tag="IWK54_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9593296.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20731..21036)
                     /locus_tag="IWK54_001165"
     CDS             complement(20731..21036)
                     /locus_tag="IWK54_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9593297.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21125..22156)
                     /locus_tag="IWK54_001166"
     CDS             complement(21125..22156)
                     /locus_tag="IWK54_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9593298.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22363..23237
                     /locus_tag="IWK54_001167"
                     /pseudo
     CDS             22363..23237
                     /locus_tag="IWK54_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            complement(23389..24231)
                     /locus_tag="IWK54_001168"
     CDS             complement(23389..24231)
                     /locus_tag="IWK54_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9593299.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24323..26200)
                     /locus_tag="IWK54_001169"
     CDS             complement(24323..26200)
                     /locus_tag="IWK54_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9593300.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26197..27618)
                     /locus_tag="IWK54_001170"
     CDS             complement(26197..27618)
                     /locus_tag="IWK54_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9593301.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27621..31010)
                     /locus_tag="IWK54_001171"
     CDS             complement(27621..31010)
                     /locus_tag="IWK54_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9593302.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31267..32658
                     /locus_tag="IWK54_001172"
     CDS             31267..32658
                     /locus_tag="IWK54_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9593303.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32731..33378)
                     /locus_tag="IWK54_001173"
     CDS             complement(32731..33378)
                     /locus_tag="IWK54_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9593304.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33386..34072)
                     /locus_tag="IWK54_001174"
     CDS             complement(33386..34072)
                     /locus_tag="IWK54_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9593305.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34078..35151)
                     /locus_tag="IWK54_001175"
     CDS             complement(34078..35151)
                     /locus_tag="IWK54_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9593306.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35373..36047
                     /locus_tag="IWK54_001176"
     CDS             35373..36047
                     /locus_tag="IWK54_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9593307.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36052..37392
                     /locus_tag="IWK54_001177"
     CDS             36052..37392
                     /locus_tag="IWK54_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9593308.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37541..38527)
                     /locus_tag="IWK54_001178"
     CDS             complement(37541..38527)
                     /locus_tag="IWK54_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9593309.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        11970 a         7073 c         7336 g        12461 t
ORIGIN      
        1 taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa tcgagagtac
       61 aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag tattttcgac
      121 gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt ttattgccgg
      181 aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca accagcaaat
      241 tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg gcgatatcgg
      301 tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt tttaatgatc
      361 ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt tatgatgtac
      421 atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt ttctttgatg
      481 gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat tttgataagg
      541 agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta gaagaagaat
      601 tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac attggcgcga
      661 tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat gtttgatctt
      721 caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat tcgattagac
      781 ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg tatttcagca
      841 gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt gagactttcc
      901 catagataca actttttaga gtgcatttct aaagcttgta tctatgctag acatgaacat
      961 tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg accgctttgg
     1021 ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa aagattgact
     1081 ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa ttattttagt
     1141 ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa gtacttcgac
     1201 gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc atccggcatt
     1261 agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa aagaaggaga
     1321 aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag ccacaatgag
     1381 tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac ctgccgctca
     1441 atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa cggatcctgt
     1501 gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag gtgtttctga
     1561 tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa atgcaaaaaa
     1621 agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag cacaagcaga
     1681 agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt ccactaatga
     1741 gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg ttccattaga
     1801 taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag caaatatccc
     1861 cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa tcgggatcaa
     1921 tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga gtggaaaaga
     1981 aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga tcaatcaatc
     2041 tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag ccaaaatcat
     2101 tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt atcagctatt
     2161 tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta tcggatctta
     2221 gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt ctgtgtgaca
     2281 gctattactc atggggtagc gccgattgta gctacattat taggcgtagg tgctggcatg
     2341 atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt tattttagca
     2401 gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg aacaccaaat
     2461 ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg gttacctgat
     2521 tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc tgtactgcta
     2581 ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa cgaaacgatg
     2641 gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac tttgtcgaac
     2701 ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc agatgtaaat
     2761 aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga agtgatcttc
     2821 caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat catttatcag
     2881 ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa gttgttctcc
     2941 gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact gaacttaaaa
     3001 acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa aatggcagaa
     3061 aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt aaccttacaa
     3121 tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa agcacactgt
     3181 tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt aaagatcagc
     3241 tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt ttccaagacc
     3301 ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt gcacaaaaaa
     3361 gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa gattttttca
     3421 gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca gcgggtgaat
     3481 tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca gctcctgagt
     3541 tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa gcattaatgc
     3601 gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc acgcatcgaa
     3661 tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg aaaatcgttc
     3721 aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg cagttctttg
     3781 aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac ttgaaaactc
     3841 attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa acatatttgg
     3901 gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat tctttgctct
     3961 gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt cgtacttttt
     4021 cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga ataagatgat
     4081 ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca gtttatcccc
     4141 attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt tagctttcca
     4201 agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg atatgttagg
     4261 tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg aggtaagcca
     4321 ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc gtttgattgg
     4381 ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga tttcgatcaa
     4441 aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca ctgttcaagc
     4501 tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa cattcgctca
     4561 agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa aagttcgtga
     4621 ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc tagtcaatgc
     4681 aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata taagcatgat
     4741 gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac gtttattaaa
     4801 agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga tctaccaagg
     4861 agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga ttgaaaagct
     4921 gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac ggtctcaaga
     4981 aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa ttatcaaaaa
     5041 atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga aacgaggagg
     5101 aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga aacctctgaa
     5161 gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta tggaaaatga
     5221 ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt tgaaccaata
     5281 cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa ataaaaaatt
     5341 tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat cgtggaaaaa
     5401 aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat tttttattgg
     5461 taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa cctttcatgg
     5521 gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata cacgcggaga
     5581 agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt cccataagcc
     5641 gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag gacaaaataa
     5701 gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt tagtagaaaa
     5761 ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg aaaaagtgat
     5821 cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga atctggatgg
     5881 tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc agtacgcacg
     5941 tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc aagtaatgaa
     6001 cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt atgcagctgg
     6061 taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga aaaacctctt
     6121 ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag acggttcgtg
     6181 gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc aaatgaatca
     6241 atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa gaagctgcgc
     6301 aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt cttatggtag
     6361 atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa aaatgagtcg
     6421 cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg tatcaccaca
     6481 tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa aaatctaatc
     6541 acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac gccagaagga
     6601 attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac aagaaatgaa
     6661 gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga gatgccactg
     6721 gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg catcaactgg
     6781 cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca agcagcaaac
     6841 ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg tatctttttt
     6901 ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa ttaagtaacg
     6961 gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca aaagcctatg
     7021 ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc gatccattgt
     7081 ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg gttgctccag
     7141 gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt ttcccagaaa
     7201 ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta aaacgagaat
     7261 tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt gaagccatcg
     7321 cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat ttacgtttat
     7381 ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc caacctggaa
     7441 agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat tggctgattg
     7501 gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt gatggcagtt
     7561 tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg caagaagaaa
     7621 tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg acaaacaaag
     7681 ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa gagttaacga
     7741 aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt cttgaagtta
     7801 cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt ttgcagacaa
     7861 agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct caaggattgt
     7921 acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca gaaaatgagc
     7981 atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata gaagaagtac
     8041 agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt caagaacatc
     8101 atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag ctttccaaat
     8161 tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt gctgcagatt
     8221 atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta aaaagcatcg
     8281 gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca aaaaagaaag
     8341 aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa ctgaaaagta
     8401 tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc acagcaatga
     8461 aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa gaagtagaag
     8521 gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag aagcattaca
     8581 tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt tgtaaggcag
     8641 tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa gggtacttga
     8701 gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc aaaaaattca
     8761 tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt tcagatgaga
     8821 ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta ggaagatgtc
     8881 caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc ttgaatccgg
     8941 aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa acgaatctca
     9001 gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact ggtaaaaaag
     9061 ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt tcttttccat
     9121 ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca agaaaaaata
     9181 atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat tccgaaaagt
     9241 gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa acgttggggt
     9301 aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc attcattttt
     9361 aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc taaagtatcc
     9421 tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt caccaacgtt
     9481 gattttatcg actttgactg caggagctaa aagcgctact tatttttttc ataaaacgac
     9541 aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag cggctatgat
     9601 cattatttat tttctaccag ttattacaca agtatttcat caatattttg gttaagaata
     9661 cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt ttcgtaagaa
     9721 aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata atgtacttac
     9781 tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa agtataaaat
     9841 tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag aattcatggg
     9901 tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg tacgaatgat
     9961 caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga atcaatatga
    10021 agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga aattatcaaa
    10081 atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt aaaagatgca
    10141 aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca agaactctat
    10201 cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa acatcctttt
    10261 cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca attgaataat
    10321 tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat taccactagt
    10381 tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca aaccgcaata
    10441 aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta aggaatatgc
    10501 tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg aaaatatgga
    10561 ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag gaaatagtgg
    10621 tttagaacta gtgggctgat tttttatata ttttagttta cataatatat attatacaaa
    10681 gtagatttaa aaaatgataa acctctctcg aacggttttt tatcaaaaat cgtcattttt
    10741 ccatcaaaat ctgcttatgc aaaattataa aaaaattttg cagtttttct tacttttttt
    10801 gtcgtttaat tgaatgataa aaaccagttt ctttattcag ttaatcaaat atgaacgaat
    10861 tagatatgaa tcattgtatt tgccgttgcg gaaggatttt ttaattaacc acagataaac
    10921 attgataacg attcgcttat tgtaatcaac agattaaatt tcatatgttc atatgtacgg
    10981 atacattttc ggaccgatta attttaacca ttctgtttat ttgcttacct attagatatc
    11041 aataaaaaac tgcttaacag ctatttcagc tgttaagcag ttttaggatt tttagttcat
    11101 attcattgtt tcttataata cagtgttcaa tacttctctt aaagcacttg ttgatttgct
    11161 gaacattgct ttttcttctt cgttgattga taattcaaca acttcacgaa caccattttc
    11221 gtcaacgatt gatggcacac ctgtaaagat atctttttcg tcgtattcac cagttaaata
    11281 agcagataca ggcaatacag cttgttcgtt attcaagata gcttttacga tacgtgcagt
    11341 actcatacca ataccatagt aagttgcttt tttacgatca atgatctcat aagcagcatt
    11401 acggacttta tcagcaatca catctaattc atcttgggca atacgatggt ctttttctac
    11461 aatttcaaac actggtttac caccgacagt tgtatgtgac catgcagcaa cttctgaatc
    11521 accatgttca cctaagatat agccgtggac gctgcgtggg tcaactttta atttcaatgc
    11581 gatttcttta cggaaacgag ttgtatccaa tgttgttcca gtaccgataa cacgtgatgt
    11641 tggcagacca gattcgttcc aagcgatata tgtcaaaata tctactgggt ttgaagcaac
    11701 aacgataata ccgtcgaatc ctgatcccat gatttctttt acgatttggc gcatgattga
    11761 cgcatttgtt ttaactaaat ctaaacgaga ttgaccaggt ttttggttga taccagctgt
    11821 gataacaacg atatcagcat ctttacattc ttcatagcca cctgaccata cagccacatt
    11881 ttcatcgccc caagccatac catctaaaag gtccaatgct tccccttctg ctttttcttg
    11941 attcacatcg atcaatacta attcattaga gattccttgg ttgatcattg cataagcgat
    12001 acttgttcca acaaaacctg taccgacgat tactacttta cgacttgttt ttttcatgaa
    12061 taaacagctc cttttcataa ctatttgtta ctttcatgaa aagtatagtc ttgtttattc
    12121 attttatcaa catgttaatt ataatagttg ttattataat ttttgatgga aacgaattcc
    12181 tgaatacgca taacagataa atgacaaatg tttacaccta tatcccctga taatacaacc
    12241 aaaagtttag aactgaaaat aaatccaaaa aaattcgctg caacgatata ataagacagc
    12301 aatcgcacct aaaacaattt tccttcatat gctttagtga aagattttct tcttaatgtt
    12361 tatttctcca ttcattggga gtacatgttg aatacttctt gaataatttg aataaagaag
    12421 tcgtttgttt atatccgata cgattagcga tttcttccaa cgaaaaactc gtttcttgaa
    12481 tcagttccaa cgcaaagtcg aattttttct ttagaaccag ttcttgaaag gaagcatcaa
    12541 aatacatttt caatttatta ctaagataga ccggattgta gccaaaatga gctgctgttt
    12601 cttttaacgt acaagaagta aaatgcttat cgatataatc taatactcta agcatctcat
    12661 tttctgcgca tccatctgct gatgaaaagc tttgttgttc taataaatga gtcaattcga
    12721 tcagtaatag tgatgtatag agatttaacg aacggatctt atgactgtat gtatcaaaat
    12781 aaatcaacag catattttca atcaaatgcg tcaaacgact tgtttttccg ccgcgaaaat
    12841 gaatgaattg tgtatgttcc cctttttctt ttgatgcatt tatcaaaaaa gaagaaagta
    12901 gattatttgt gcttgccaaa tgattcaata tctctgtaga taaggtttct tcttttaata
    12961 agatgttgat taaaatatcc tcttgccctg tgtattcgat tctttgagta gcttctttat
    13021 caatgatcac cacgtcactt tcttccatct ctatcctttc tccattaata atttgtaaac
    13081 atgtaccgct atacatataa ttgatttcaa caaaatcatg tgtatgctcg ggaacttccg
    13141 catatcgacg gtgtttactg atatgaatat ttccctgttg aaatagatcc cctcggtgga
    13201 tttttggcgg tgtctgaaga tggtaagcta agtttaaatc attgatattc attccttgtt
    13261 cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa aaatgtctaa ttcagctagt
    13321 tccaattgac tccctccttt ttgtaagcgg tttctttcgc ttattagttc acgctgtact
    13381 gctattatat gcaaagctat ttttggtttc ataaagactt tctatggttg ggacatgaac
    13441 ttcctgtgat tccgctattg agagaataac cacttcatac tatatttttg ataagcgctt
    13501 acaaaaatga aaggaacatg cttatgaata aaaagatgat gaaattttct ctgctgtcag
    13561 tctctcttat tttgacttct gctggtgcag tcacgtctgc aattcccagt atgtcagcta
    13621 cttatgccac gcgttcttta tctgctgttg aattattaac gactgctccc tcttttatgg
    13681 tcactatttt tgttttgatt agtagtttta ttgctaggaa aatcggacaa aaacaaactg
    13741 tgatcatcgg attagtgatt gcatcgattt gtggtatcct ccccctcttc tcacaaagtt
    13801 acagtattgt cttagcttca agagctggac taggtattgg ctttggattg atcaattctt
    13861 tggcagtcag catgattgct gccttttatc acggagatga acgagcctct atgattggtt
    13921 ttcaaagtgc atttcaagga tttggtgcag ctttaatgac atttgttgca ggacagctag
    13981 tacagttcgg ttggcaatat acctttttag tctacgccat cagcttacct attcttgtct
    14041 tgtttgtttt gtttgttcct acacctgaaa caactgaact tcaatcgcat ggcacagccc
    14101 caacaagaca agtaatcaaa acgaatccta aaatgattag ttatggtatt tttctcttgc
    14161 tggtcatcgt ggtatataat gctgtatcga ttaaattagc cacagtcctg acgacttatt
    14221 ctctaggaaa cgaaactaat gcagcgaata tcttaacaat catgcaattg gcaagtatgg
    14281 ctgccggagt ttgttttggc tggcttcaga caaaagcaca tcgctatatg cttacacttt
    14341 ctttactact gatgggaatc ggctttgtat tgattgccac tgtgataaac ctgtatgtga
    14401 ttgggtttgg tgcgatccta acaggaattt ctttttcgtt attcattcct tacttattca
    14461 accaagtaag tatccaagcg cccaagtacg cggagagttt caatacttca atcttattgg
    14521 ttggcgctaa tttaggtact ttcattgcac catatggttt gctctttctc tcttttgctt
    14581 ctcttgggag taaaacactt gctgttttcg tcaatggcag tatcttgctg ttgattagcg
    14641 cgcttattag ccttctagta gtacacagga cgaatagaaa gacagtgata agagtagatg
    14701 aagaaataaa agaacaattg acaacaaaat aaatttgatg gaggtcgtgt aaaatgacaa
    14761 atgaaaaaca taaagataga tggctttggt atcctggaga ctttgagatc cgccatggac
    14821 tgctacaaaa tttccaacgg gaagaacgag gttttgactg gccagcgtac tggtacatgg
    14881 atgattgcca tcggaacgtc aagtttaaga gatattactt tcttgatcaa ccttccatgt
    14941 ttaaagtgac gatccaagga gttgggtatg tagaaattaa tggacaaaaa catccatgtg
    15001 gaaaatggct gacttgtcca gctggtaaag caaaaattcg tatctttgta ggacatacat
    15061 cagggctacc agcgatgttt atcgagggag acgaagtaaa atcagatatt ggctggacag
    15121 caagtaattt tattgaagaa tatccagctg gctggagtcc gctttatgtc gatatcgcaa
    15181 aagatccaaa tcaaatctat tatcaaaaag aatgcgtcca tccagtcact gaaacggaga
    15241 ttggcggagg aatcttattc gattttgggc gagcagtgaa tggctgtgta gaactaatat
    15301 ctctggatga ccatatcgaa tctatcactc tatgttatgg agagtccgat gtagaagcta
    15361 tggatatcga ttattgctac tataagcaaa taggaatgac aagaaaaatg aaagctcgta
    15421 aacgcgcttt tcgctatttg tttataccag aggtttctac tggaatggtt caacttcaag
    15481 tttttcatga atttttatct ctttcgaaac aagctgcttt ttcaagcgat tcttctttac
    15541 tgaatcagat ctggtcagta tcagaagaag ccttcaagtt atgtagtgat ttgtttttta
    15601 tcgacggtat caaacgagat cgctggattt ggtctggaga tgcgtatcaa tcattcatga
    15661 tcaatcaata tctttttttt gacgaggaaa tcaataaacg aacgatatta gcattaagag
    15721 gacaaaatga aatcaagcag catatgaata ctatcgttga ctattcgatc ttgtggttga
    15781 ttggtatcga aaaccactat atgatgacga aagactatga atttttacgc atcgtctacc
    15841 ctaagatgca atcattaatg agatacttga tggagcaaac gaatgaatta gggtttattt
    15901 atgggagagc aaaagattgg atatttattg attggtctga aatggataaa gaaggaacgc
    15961 ttgctgcaga acaagtctta ttactgaaag cctatcattc catgatcact tgcggagata
    16021 tcttagctga aacatatgga gagaacgaaa attatcatac tcatctctat caagaaaaat
    16081 atcaactatt gaaacaaaac ctatttgact acttttggga tgaagaactt ggcgctttta
    16141 tcgattgcta tgaatctggg agaagaaatg tgacgcgtca tgcgaatatt ttcgctgttt
    16201 tattcgattt tgctaacaaa gaccaacagc aatcgatctt aaagaatgtc ttattgaatg
    16261 acactatcac acagattact actccatatt tcaaattcta tgaacaagat gctttatgca
    16321 aactcggaca gacgaatatc gtctaccaaa cgattcttga ttattgggga gggatgttat
    16381 ctcatgatgc ggtaactttc tgggaagaat acgaccctac tcaaacagga aatgaaaagt
    16441 acagtatgta tggagatcct tatggaaaaa gcttatgcca tgcatgggga gcaagcccaa
    16501 tctatctttt aggacgctat tttatcggtt tgtatccaac aaagccaggt tatgaagaat
    16561 ttatgataca acctaatcta gattcatttg aaacactgaa ttgtacgcta cctatcaaag
    16621 atggaaagat ttcgcttgaa aaaacaaaag ataagatcgt gattacttct gatagaacag
    16681 gcggaacagt gaaaatgaat ggtaaaagtt atcctcttct agcaaaacag ccacttgtct
    16741 taacaagcta ttcaaaaata aatgcataaa acaaaaaact gcttgcgaca tatcagaaga
    16801 ctttaaatct tcttctgata ttgtccaagc aatttttatt ttgaagtttg cttaattctg
    16861 ttctttagaa acaatgtttg ttctttttcc ggcaaaaaga aagctagata atacgacaag
    16921 taaaaagaaa gcgatgattc caactgtcaa ggctatttga ccgtattctc ctagtttccc
    16981 aagtacaagt cctggtacta gataatcagt atcggcaaat gtagaagaag cgattttaag
    17041 atctcctaat actggaagga taaataatgg cagccaacta atcaataagc tattgaaagc
    17101 agaacctaat accgcccctc ttctaccgcc agtcgagttg ccgaagacac cagaagcagc
    17161 tccgcagaag aaatgtccta ctaccccagg aatgataact gtagtttgca agcctatcat
    17221 agcaaacatg gatagtgttc ctacaaagaa gctaacaaaa aagccaatta atacagcatt
    17281 aggtgcatat ggaaaaacga taggaacatc taatgctggt ttagaatcgg gtacgagttt
    17341 tttagcaatc ccttggaatg ctggaacgat ttctgccaac accattcgaa ccccttgaag
    17401 gacgataaca aacccagctg tgaatgttcc agcttggatc agtgcaaaga ttacagcgtt
    17461 gtctccattg cttagttctc tttcaacaaa tccaggacca gctaacaaag caagtgcgac
    17521 atatacgatc gacatcaaga gcatgatcga tacagtagaa tctcgcaaaa agctgaaact
    17581 tttaggaatc tttatgtcct ctgtagaacg gtctttgttt ccaaaaaact ttccgatcca
    17641 tccactagaa gcataaccga tatttccggt atgccctaac gcaacttgat tgtttccagt
    17701 gatgttcttc ataaacggtt gacaaagagc aggcgtaata gttaatagtg ttccttcgaa
    17761 gaccccgcct aataagatca tccacaaatt tgcagtaaac cccgcgctta ctaaaatgac
    17821 tgctgttaga caagaaacat aaagcattgc ttgacctgta agaaaaatga atttatatct
    17881 tgtgaatcta gcaagtaaaa tattcacgat catccctact agcatgatca aagcagtagg
    17941 tgtaccgtat tgttgcaaag caatcgccac tactgcttca ttgctaggaa ctactccttg
    18001 agtatgcaga gctgtttgga acatcgaagc aaaaggggtt agtgaattac tgattaaacc
    18061 agcaccgccg atcagtacta ggaatccgac aaatgttttg atagttcctt ggatcacttc
    18121 tgaaaatttc ttcttctgaa gaacgagacc aagcagtgca atcaaagaaa cgaggatgga
    18181 gggctgactg acgattgaca caaacaaatt caacaattta tccattgata gttcctccct
    18241 tgaatcattt ttattctttt gacaagatct tcgttacttt ttcttttact tcttcgctgt
    18301 caatgatcga ttgcaaaggt acgagctttt catcaggtat agaagttagc gtatctttca
    18361 aagaatgctc aatgaagaaa taatcagcca tatcgctaga tacacttcca acatccgtat
    18421 gacttacttc aaactcgtcc acatctgctc caagatctgc aagtgcttgt ttgatgttca
    18481 tctctaccat aaaacttgtt cctaatccac tttgacatac tgcaaaaatt ttcatatcat
    18541 tcgtctcctt ttcttatgat cgctaatatt tcttcttttg ttgatgcgtg taataaatct
    18601 tgaagatttt gtttattaga aagtagcttt gttaaatttg ccaaagcccg aagatgtgcc
    18661 tcattatcga tagcagctaa acagatgaac aaactaatgg gatgggctgg attatcaagc
    18721 agatccacag gttgttgaag tttcagcaaa gacatcccta cttcgtttac gccatcttct
    18781 ggacgagcat gaggtaatgc gatgtgatcg ccaatatgga taaacgcacc atactgctcc
    18841 acgcgctcga tcatagcttt gatataactt tcttctattt tattttctga taatagcggc
    18901 tgtgcggcaa atgtgattgc ttcttgccag ttttttggtt gatccgataa ttggatcatt
    18961 tcttctgtta ataattcaga tagcatagga cgttgatcct ccttcttttc taaaagtttg
    19021 ttcattttac gatttaatac ttgatagagc ttttcttcgt ctacgccttc ttttaattcg
    19081 atataaggca gtagcgccga caacaattct tgggcagaag gcattgaaaa tcctggtatc
    19141 agccaatctc tttgtacttg attgatcagc tgattttttt ccaagtcagt catgattgga
    19201 tgcagaacat agagagactt tttcttatca gtaagtgtga gaggtacagt agaaaaaata
    19261 atatcgtaag aagtttcgtc aacctcgtgt aagcggtcag ctgaattagt ctctgtaaaa
    19321 aagatcatcg ggaataattg ctgaagttca ctttttaaga tcaatgaaga gctaatgcca
    19381 cttggacaga cgatcacggc tcttattttt ctatttcttt cttctgcgtc cgcttttctg
    19441 atctctccgc caaatagtat agtgaaaaaa ccaatctctt cttcagggat gctcttccct
    19501 attcgtcttt ctaacggaag taatgctttt cttgtcattt cgtagatgga agcatattga
    19561 tgcttaattt gatcgatcat cacattaggt aaataaaaag aataggaaat acgaaaatag
    19621 gcagggacca agtgataaaa gacattcatc aataattcac gaaacgaagc aaattctacg
    19681 gccatcagga attccatgcg atggatgact tctgaagaac attccagcaa aaaatcaagt
    19741 gcaggctcct ggacatctcc ttcaacgatc gatgctaagc aaagagagaa aaatataatc
    19801 tccgaatttt gatgttcgga atagccaagg ccagtcaata cttgtcgact aatgtcagtg
    19861 actgcaagag aagccaacag ttcagcatct tttaccagtt ccatggatgt ttttccaatt
    19921 cgaatggttg tcaaactcaa ataataagct aattcatcca ttcgatttgg tgcgaatcga
    19981 agatctgata gtgaaatcgt tttatctagc aaatctctaa ctttggcgta ataatcaatg
    20041 gaaagggttg cggttaaata cgtgagccca aatcttccag caagtgactg catcaaacga
    20101 gcagtgcgat tttttgcttc tcttcttaat attgcttctg accctagcag cctaaaacca
    20161 gttttcctag tatacgacag ctgacaatct tccacttcta attcatttct tatctttttg
    20221 atatctgaca ggactgtccc tttagaaacc tgaagaaaat cttgaaaatg aaaaacagaa
    20281 acagacgttg cagtcgaata aaccatcaaa taaagaagat ctcttcgctc ttgttcggta
    20341 tacacagctt caaatcgagt aatatccaaa aatttttctg cccagtcgat tgctcggaat
    20401 ttctttatac ggatacgggt atcatccaca tattcggcaa aaggagttaa ttgtttgatc
    20461 gcctcgcttg tatttacttc gatgatctct gctgcttttt ctagactgat ttctcttggt
    20521 gttttcagga gataactaat tttgtagttc acagcgctgt tcacctccta cttatattct
    20581 agtatggtag cgcttttaaa tcgaggggtg tttggtacaa cttacagttg gatttttttt
    20641 acgactgtac cgaacgattt ctatcgaatg gatacagaaa aaaagcaggt gaccaaatgt
    20701 ctcctgcttt tatgaattga tttcgtccga tcaatgatga tcatgtttat tttttgaggt
    20761 cagttcttca aatttttctt tgacatcttc aaccgctttt tcgatatctt tttcaaagtg
    20821 attgggctca ttaggctttg tttcctcttc tacagaattt gaaccagata aagctgtttc
    20881 tttattttct gtttcaccag agtttgtgcc agctaatgat tcttctttgt cttctgtttc
    20941 accagaatta gttcctgcta aagccacttc ttcttctgtt ttaggggcca ttgaacctct
    21001 taatcgtttg tgtttttcac cgtcataatt tgtcatgttg gttcctccta gtagttctat
    21061 actttttcat cgtacaataa gacagtttat gcgtcaaaaa acaaacccga ttcttacaaa
    21121 tatgtcatat ttttccttct gattcttgaa tgcttttgat tccttcttcc aaaattttca
    21181 tttgtaagat tgtttcttca tctttgatga ctttttctgc gccattaact aatgtctcgt
    21241 aaatcccttc gtagacacgg ctgtaatcac caacctcgga aattactttt tcttcatgat
    21301 aactcccttg ttcatctata taggtcaata caccataatg ttcgggcaaa tcgataccaa
    21361 agtcttcatg atcaggcatg taaaacattt tcagatgttc ttcttgtcga tcttttgttt
    21421 gtttcacgaa catccctttt tttccgtaaa cgacgaaact tggacgttct ttgatgcgga
    21481 aataactgga ttttatcgaa attttcatat ttccatagaa aaaatccaga tcgaagtaat
    21541 cattcattcg tcctttccct aaaagttgac gtacatcata gctgatatca tctggttcgc
    21601 cgaaataaga aagaacttga tctactgtat gacaagcatg tccatataaa tagctgttcg
    21661 cagtagaaaa ttcatgcgta ttttctggta cttccggtct gaaataatca taatgcattt
    21721 ctacttctaa aatatcccct aattttccgc tttcgattac tttttggact gtcaagaagt
    21781 cagaatcgaa ccgtctgttt tggtaacatt ggataaatag acctttttct tttgctaatg
    21841 caaataactc ttctgcttcc tttgacgttt ctgcaaaagg tttctctact agtacattct
    21901 taccatgttc gagtgccatt tttccatatt cataatgaaa ttgactaggt gtagtgatga
    21961 caactaattg gatctcagga tcatcccaaa gatcagacag ctgatcagta tattcgatgt
    22021 cttcgaatgt tttccacact ggttcagatg gggaaaaaat ttttttgata ttgattttgt
    22081 tcgtaagttt tcttgaaaaa ggcaaatgat agcgatttgt actctttcca ttgccaagat
    22141 aagcgattgt aagcatgagt ggtgcctcct tttattgatg ttttcagtat atcctagaaa
    22201 agcgcagaaa gtatttataa tagctattct ggtaaaaaag ttcagaaaaa cacttgttat
    22261 cattcggttt ggtgaaatta atgttcaaag taaggataaa aatcaaaaag atagtagcgg
    22321 aaccaagtgt ttatcagtaa gatagaattt ggaggtcaga tcatgcttat tgaagaaaaa
    22381 ctaacaaaac aagagttgtt taccacgaca gagaaacgga ttgtcgatta catccgtagg
    22441 aatatcgaag ctgcagtcta catgacgatt gaagaattag caaaagctac gtatacttcc
    22501 cattcagcta tcattcgttt atgcaaaaaa atgggattca gcggatttaa agaattccgt
    22561 ttcgagctgg ggcgggaagt tcatcaattg attgctcagt tcaatcaaac gattgatcct
    22621 aattttcctt tttcaagtga agatagcact cagcaaattg caaagaaaat ggcggattta
    22681 tcgatccagt caataaaaaa agcacaattg cagattgaac atgaagattt agatcaaatc
    22741 ggtaaagtat tgaacaaggc acagcgaatc tttctttttg caaaaggtga ttctcaaatc
    22801 acggcaagga aattccagaa taaaatggtc aagttgaata agtttttgat catggctgaa
    22861 gaatacagtg attcttcttg gaatgcagcc aatttgacta gcgaggattg cgccattttt
    22921 attagctaca gcggtcgcat ccatcattat gaacgaataa tgacctattt gaagcatgta
    22981 gaggccccta ccttgctgat taccggaaat cagcattcag aaatggccaa acaggctgat
    23041 atgtgtcttg ttatatcgca ggaggaatat gattttgcta aagtagcgac ttctcctctc
    23101 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    23161 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    23221 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    23281 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    23341 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    23401 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    23461 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    23521 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    23581 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    23641 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    23701 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    23761 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    23821 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    23881 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    23941 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    24001 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    24061 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    24121 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    24181 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct
    24241 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    24301 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    24361 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    24421 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    24481 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    24541 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    24601 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    24661 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    24721 cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa
    24781 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    24841 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    24901 tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa
    24961 ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt
    25021 tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag
    25081 cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat
    25141 cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga
    25201 catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg
    25261 tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt
    25321 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta
    25381 aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga
    25441 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt
    25501 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag
    25561 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    25621 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    25681 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    25741 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    25801 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    25861 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    25921 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    25981 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    26041 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    26101 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    26161 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    26221 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    26281 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    26341 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    26401 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    26461 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    26521 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    26581 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    26641 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa
    26701 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    26761 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    26821 aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt
    26881 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    26941 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    27001 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    27061 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    27121 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    27181 tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    27241 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    27301 cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc
    27361 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    27421 taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg
    27481 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    27541 ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    27601 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    27661 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    27721 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    27781 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    27841 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    27901 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    27961 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    28021 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    28081 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    28141 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    28201 gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt
    28261 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    28321 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    28381 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    28441 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    28501 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    28561 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    28621 agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca
    28681 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    28741 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    28801 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    28861 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta
    28921 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    28981 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    29041 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    29101 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    29161 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    29221 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    29281 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    29341 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    29401 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    29461 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    29521 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    29581 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    29641 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    29701 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    29761 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc
    29821 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    29881 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    29941 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    30001 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    30061 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    30121 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    30181 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    30241 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    30301 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    30361 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    30421 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    30481 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    30541 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    30601 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    30661 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    30721 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    30781 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    30841 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    30901 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    30961 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    31021 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    31081 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    31141 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    31201 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga atgaaactat
    31261 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    31321 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    31381 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    31441 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    31501 aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    31561 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    31621 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    31681 agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc
    31741 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    31801 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    31861 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    31921 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    31981 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    32041 gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac
    32101 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
    32161 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
    32221 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
    32281 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
    32341 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
    32401 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
    32461 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
    32521 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
    32581 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
    32641 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
    32701 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
    32761 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
    32821 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
    32881 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
    32941 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
    33001 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
    33061 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
    33121 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
    33181 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
    33241 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
    33301 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
    33361 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
    33421 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
    33481 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
    33541 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
    33601 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
    33661 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
    33721 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
    33781 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
    33841 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
    33901 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
    33961 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
    34021 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta
    34081 acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc
    34141 tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc
    34201 aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag
    34261 aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt
    34321 tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta
    34381 catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa
    34441 ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa
    34501 ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc
    34561 attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca
    34621 cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc
    34681 gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc
    34741 tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt
    34801 cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt
    34861 gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat
    34921 gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga
    34981 caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc
    35041 attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat
    35101 caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc
    35161 ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt
    35221 gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga
    35281 taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata
    35341 aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg
    35401 ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag
    35461 tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct
    35521 tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa
    35581 aaatacagac tccagtattg attttgactg caaaagatag tttggaagat aagattgaag
    35641 gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca
    35701 tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt
    35761 ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc
    35821 ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt
    35881 tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag
    35941 ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag
    36001 aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga
    36061 gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt
    36121 atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa
    36181 acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa
    36241 agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac
    36301 atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg
    36361 tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa
    36421 gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac
    36481 agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat
    36541 ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact
    36601 tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt
    36661 tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa
    36721 tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg
    36781 gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa
    36841 gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg
    36901 aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa
    36961 caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt tattctccat
    37021 aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt
    37081 ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct
    37141 gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa
    37201 gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact
    37261 ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag
    37321 atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag
    37381 cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt
    37441 ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct
    37501 ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt
    37561 cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg
    37621 tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg
    37681 tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca
    37741 atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt
    37801 cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt
    37861 cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa
    37921 ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg
    37981 tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg
    38041 ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt
    38101 ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt
    38161 ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat
    38221 ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat
    38281 caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc
    38341 gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg
    38401 cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt
    38461 caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct
    38521 cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt acttataaat
    38581 ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg attaaaacaa
    38641 tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaata aaatgtcatt
    38701 tgtttctata tgaattttta tgaaaaattt catataattt ccactcgttg tgatggttta
    38761 atttatccaa tccatcaata agcaaacaac ttatataaaa aaggattaat tctcaatata
    38821 attgctccta ccatagaata
//
LOCUS       DACDTH010000022        37235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDTH010000022 DACDTH010000000
VERSION     DACDTH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.022"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            80..829
                     /locus_tag="IWK54_001179"
     CDS             80..829
                     /locus_tag="IWK54_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9593310.1"
                     /translation="MVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQL
                     VETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPK
                     TGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1253..1423)
                     /locus_tag="IWK54_001180"
     CDS             complement(1253..1423)
                     /locus_tag="IWK54_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9593311.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1617..1796)
                     /locus_tag="IWK54_001181"
     CDS             complement(1617..1796)
                     /locus_tag="IWK54_001181"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593312.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2183..3295
                     /locus_tag="IWK54_001182"
     CDS             2183..3295
                     /locus_tag="IWK54_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9593313.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3373..3666)
                     /locus_tag="IWK54_001183"
     CDS             complement(3373..3666)
                     /locus_tag="IWK54_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9593314.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3834..4148
                     /locus_tag="IWK54_001184"
     CDS             3834..4148
                     /locus_tag="IWK54_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9593315.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4169..6148
                     /locus_tag="IWK54_001185"
     CDS             4169..6148
                     /locus_tag="IWK54_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9593316.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6161..6487
                     /locus_tag="IWK54_001186"
     CDS             6161..6487
                     /locus_tag="IWK54_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9593317.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6503..7021
                     /locus_tag="IWK54_001187"
     CDS             6503..7021
                     /locus_tag="IWK54_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593318.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7021..8373
                     /gene="celB"
                     /locus_tag="IWK54_001188"
     CDS             7021..8373
                     /gene="celB"
                     /locus_tag="IWK54_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9593319.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8457..9962)
                     /locus_tag="IWK54_001189"
     CDS             complement(8457..9962)
                     /locus_tag="IWK54_001189"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9593320.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10054..10644)
                     /locus_tag="IWK54_001190"
     CDS             complement(10054..10644)
                     /locus_tag="IWK54_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9593321.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10823..12469
                     /locus_tag="IWK54_001191"
     CDS             10823..12469
                     /locus_tag="IWK54_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9593322.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12518..13237
                     /locus_tag="IWK54_001192"
     CDS             12518..13237
                     /locus_tag="IWK54_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9593323.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13716..14663
                     /locus_tag="IWK54_001193"
     CDS             13716..14663
                     /locus_tag="IWK54_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9593324.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14675..15595
                     /locus_tag="IWK54_001194"
     CDS             14675..15595
                     /locus_tag="IWK54_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9593325.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15636..17093
                     /locus_tag="IWK54_001195"
     CDS             15636..17093
                     /locus_tag="IWK54_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9593326.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17438..18052
                     /locus_tag="IWK54_001196"
     CDS             17438..18052
                     /locus_tag="IWK54_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9593327.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18045..19769
                     /locus_tag="IWK54_001197"
     CDS             18045..19769
                     /locus_tag="IWK54_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9593328.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19766..21217
                     /locus_tag="IWK54_001198"
     CDS             19766..21217
                     /locus_tag="IWK54_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9593329.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21690..23843)
                     /locus_tag="IWK54_001199"
     CDS             complement(21690..23843)
                     /locus_tag="IWK54_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9593330.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23845..24891)
                     /locus_tag="IWK54_001200"
     CDS             complement(23845..24891)
                     /locus_tag="IWK54_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9593331.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25041..26330
                     /locus_tag="IWK54_001201"
     CDS             25041..26330
                     /locus_tag="IWK54_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9593332.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26343..29045
                     /locus_tag="IWK54_001202"
     CDS             26343..29045
                     /locus_tag="IWK54_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9593333.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29030..29887
                     /locus_tag="IWK54_001203"
     CDS             29030..29887
                     /locus_tag="IWK54_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9593334.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29958..30815)
                     /locus_tag="IWK54_001204"
     CDS             complement(29958..30815)
                     /locus_tag="IWK54_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9593335.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31026..33197
                     /locus_tag="IWK54_001205"
     CDS             31026..33197
                     /locus_tag="IWK54_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9593336.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33265..33606
                     /locus_tag="IWK54_001206"
     CDS             33265..33606
                     /locus_tag="IWK54_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9593337.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33882..35636)
                     /locus_tag="IWK54_001207"
     CDS             complement(33882..35636)
                     /locus_tag="IWK54_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9593338.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35647..36483)
                     /locus_tag="IWK54_001208"
     CDS             complement(35647..36483)
                     /locus_tag="IWK54_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9593339.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36485..37168)
                     /locus_tag="IWK54_001209"
     CDS             complement(36485..37168)
                     /locus_tag="IWK54_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9593340.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12013 a         6548 c         7638 g        11036 t
ORIGIN      
        1 ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg agtttgagat
       61 cgaaagaagt cagacagcgg tggtagaatt gacgaaagaa aaccgactta caccaggcgg
      121 tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag ctgtttttga
      181 gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag cagatggaaa
      241 actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc aagcgccaac
      301 ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc agacagcggt
      361 ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga caaagatcga
      421 tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      481 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag atggcttagc
      541 accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat tggatgcgac
      601 accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa caaaagagaa
      661 tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca gtggaaataa
      721 gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa tattgggtgt
      781 gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag ctaaaataaa
      841 aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat ggaaactacc
      901 gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt tgaacccaac
      961 cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa acctttgtag
     1021 taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg agtttcgcag
     1081 tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt tctacaaata
     1141 atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa taaggagaaa
     1201 aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa ggttacgcct
     1261 ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca tttggtagaa
     1321 catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat tctatttctt
     1381 taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt gtaggtaaat
     1441 tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt tatcctcaac
     1501 ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct tttggaagac
     1561 tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa agatacctaa
     1621 caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt gattttatgt
     1681 ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc gttccaatgg
     1741 cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat tggcatatga
     1801 ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac cattaatttc
     1861 ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc agggaagaga
     1921 actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac ctctgtaatc
     1981 atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat aaagcatatc
     2041 gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat atgttttttt
     2101 ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata tgatgttcca
     2161 tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga aaggacgaca
     2221 acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac aaaaaatacg
     2281 agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt tacgatcatt
     2341 attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat ttgaatcgaa
     2401 gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt ttgttgactt
     2461 tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct cactattagt
     2521 agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc attttatttt
     2581 actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg cttctttgta
     2641 cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga acaagaattt
     2701 ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag ttctccttta
     2761 cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt ttttgaaagg
     2821 catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta gtcttttgat
     2881 cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg cattttcttc
     2941 tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag tagaccgaat
     3001 cattggctca ttgattggat gtgttttatt tattggtatc agccaattta ttccttttaa
     3061 ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata gatataaaac
     3121 agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg ttccaggagc
     3181 agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca tgtatattgg
     3241 ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc attagatgag
     3301 atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg ctatcattcc
     3361 atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta atttatactt
     3421 tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt cgattgcttc
     3481 ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg gaaagtaata
     3541 gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc tttcgaccat
     3601 ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat tcactgtgat
     3661 actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg ccagaaatta
     3721 ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata ttttgaatag
     3781 aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga tttatgaaaa
     3841 aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca aagacaacgg
     3901 atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct acagaaggcg
     3961 ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa acgacgatgt
     4021 ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt agtatccctt
     4081 tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg atcgaagaga
     4141 acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc tgttgagata
     4201 tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg aattgttatt
     4261 atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag aaaaaaacgg
     4321 aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac aaagactgac
     4381 atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag aaccgcaagg
     4441 attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt tggaagatgc
     4501 cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca gtttgacaaa
     4561 ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg tttctaagca
     4621 tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta tcatggatac
     4681 tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa gccgtttttt
     4741 cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa gagaagctaa
     4801 gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt tggggatcaa
     4861 acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag tttctaagcg
     4921 agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg caggtgtatc
     4981 gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt cgaatcacaa
     5041 agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga aaagattgtc
     5101 tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac tactcgatca
     5161 tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc cattgactga
     5221 ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt tcagcaacat
     5281 gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg ctttgcatct
     5341 catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga ttatctgtgc
     5401 aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat tcggtaaaaa
     5461 tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt taaaaggaat
     5521 cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc cagttttaca
     5581 tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag ttggagaatc
     5641 tataccctat cattcctcag atagaccgat accagcattt tctctacagc aaaaaaagca
     5701 gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg aaccgaaaaa
     5761 ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag aaggatatat
     5821 aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca tattcagtga
     5881 tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg tagccgttgc
     5941 attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg tttttttagt
     6001 ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa tcgttaggtt
     6061 agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg agaatttcag
     6121 cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa aaataaacag
     6181 tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg cacggacact
     6241 gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag aagagaagtt
     6301 gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac ttctgaaaga
     6361 atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac aagatcatct
     6421 gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt atcaaaaaaa
     6481 taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg aattagaaaa
     6541 aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac gttacctgac
     6601 tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag ctaaaagttt
     6661 aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag gagaacaagt
     6721 aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt ttttgttcca
     6781 gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta cattcttgta
     6841 tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg ttgtaagcgg
     6901 tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca accaagacca
     6961 acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg agaataacta
     7021 atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc gcaacttaaa
     7081 atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat tgtaggctcg
     7141 atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg attttttaat
     7201 gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac gatggggatt
     7261 ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga acgagatgaa
     7321 caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc attctttatg
     7381 tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc tatcaatgag
     7441 acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt aggaacttca
     7501 ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg tatgtgtgtc
     7561 aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc tcgttctttc
     7621 actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa tggattatta
     7681 gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt tgttacgcag
     7741 ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc attgtggatc
     7801 gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt attagctaat
     7861 atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa caattcttat
     7921 gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc attcctcgcg
     7981 aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt attcaatatc
     8041 aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc ggttccgttt
     8101 ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct ggaaatcgtc
     8161 aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc atttgttggt
     8221 agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat cgcattcatt
     8281 atctggttcc catttatcaa attctatgat aataaattat taaatgaaga aagagaaaag
     8341 gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga actgtgacaa
     8401 aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa ataccattat
     8461 tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc tacaccattg
     8521 attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg cggagcgctt
     8581 tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac gatcaaatct
     8641 ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc aaatgctggg
     8701 tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc aaaatcatgt
     8761 cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg gccatctgga
     8821 tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg tgcatagtcc
     8881 acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg tacacgagct
     8941 tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc ggcaatgatc
     9001 gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata agcagtatcg
     9061 cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc tttatctgaa
     9121 cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg aatctggaat
     9181 agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa aacaactgtt
     9241 tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt cggatgttcg
     9301 atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa agtcaagccg
     9361 tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg agtcatacca
     9421 ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt tgtcaaatgg
     9481 gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa tgctgttttt
     9541 ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt tttacctttt
     9601 gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa agccatagca
     9661 agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga attttcgtat
     9721 ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa atattcttct
     9781 ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct tgaatcataa
     9841 cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct tgttgaaaca
     9901 aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa agagaagctc
     9961 attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact ctttttaata
    10021 tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg acacagggga
    10081 caagcggatg agtattttca taaacaatga actgactgta atagatagga gaacgaatag
    10141 tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat tggtaagtaa
    10201 aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg gtatcgttat
    10261 accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga acaaaagact
    10321 tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa caatctctaa
    10381 aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt ttcctaatag
    10441 gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg agcgttggat
    10501 agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag ctatatcgtg
    10561 agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa atataacggg
    10621 tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat aaaaggctgt
    10681 atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt gttcttataa
    10741 aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata gtaatgtttt
    10801 gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag atcaattaac
    10861 accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata tcatttcaag
    10921 attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa atgctaaggc
    10981 tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac tgatctctac
    11041 agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat tgaatgaata
    11101 tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt taggcctgtt
    11161 gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg gtggcggaga
    11221 agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc cttgtatgag
    11281 tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc tgtcgcagat
    11341 tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca tcatgaaagt
    11401 catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat ttatcggtat
    11461 gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt atacatcggc
    11521 gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt tgttagatac
    11581 cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct tcaaattagt
    11641 cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca atgcacagtt
    11701 actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg tagttattgc
    11761 attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg ttacgttgaa
    11821 agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt cattttttat
    11881 gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat tattttatac
    11941 accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac tcttcctagg
    12001 actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg cggcaattaa
    12061 ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca ttcgtttatt
    12121 tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt gtaccttgat
    12181 cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc gcaggacatt
    12241 gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa agatgatttt
    12301 tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc ttgtagtagc
    12361 aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg cagaaagact
    12421 actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag tgaatcagaa
    12481 gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata agtttttagc
    12541 agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag gaacgatcga
    12601 agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg aagatgcggt
    12661 tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt tgcataaacc
    12721 tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg atttattgga
    12781 agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag acacagaggg
    12841 cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac ctaaaaaaca
    12901 tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag atatccgttt
    12961 atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa aactccaaat
    13021 tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc atgaaggaaa
    13081 gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat atttaaaaag
    13141 aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat atcgacccat
    13201 gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc taaaagaata
    13261 agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt tcggatggct
    13321 tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg tttgttcgga
    13381 ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga ctcagagtta
    13441 gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca caacctctga
    13501 taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa aatatgagga
    13561 actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca caacctctcc
    13621 tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg aaagcgctat
    13681 aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag gggttcaatc
    13741 atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta ccgggtatgc
    13801 tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca ttcaaaaatt
    13861 ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg gtgggttggg
    13921 aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa aatacaattt
    13981 tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt gcaatcatta
    14041 tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc ttactacctt
    14101 attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt cctgacaaag
    14161 gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg tataatgaac
    14221 cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt ggttataaca
    14281 gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa gctgctatgg
    14341 ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa cttattccac
    14401 taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat tttggcctgt
    14461 tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt ttggatacgt
    14521 atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct gcaggacttt
    14581 accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc cgacgttttg
    14641 atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag tggagagagt
    14701 ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga ttgcactctt
    14761 tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta ccgatgagtc
    14821 catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat ttggttatac
    14881 gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca ctgtacttgt
    14941 tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt atgcgatttc
    15001 acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta cgatgttgtt
    15061 ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt taaaagatac
    15121 gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg tgatgcgcac
    15181 attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg atggagctag
    15241 cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta ttgcgacaat
    15301 cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac tatacatcca
    15361 agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta atattcaata
    15421 tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct cagttccagg
    15481 agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga tcagctatcc
    15541 atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag aataaagaaa
    15601 caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag aaactagcct
    15661 ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt tctggttcta
    15721 aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc ggagataaac
    15781 cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa gagatcggtg
    15841 taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg aacgtcatca
    15901 tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc aatgcccaaa
    15961 aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa gcttataatg
    16021 atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat gctttcccag
    16081 tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta ctagataaat
    16141 acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc ttgaaggaat
    16201 tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc aaagtccaag
    16261 ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg gatggagatg
    16321 attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta cgtacaatgc
    16381 atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat aaggattatc
    16441 cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat gattatggcg
    16501 atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt actgttccac
    16561 taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact tcaaaaaata
    16621 aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta ttgaatgggc
    16681 ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat gataaaatca
    16741 aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca ggaaacaaca
    16801 agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt gatcaatcga
    16861 ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac agtgtcaaaa
    16921 cagaattaag taatatttct aatgtcatga atcaatattt agatggattg aatacaggga
    16981 ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag gctggctatg
    17041 ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa taataatgaa
    17101 taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga ataaacggtg
    17161 ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga aagccatatt
    17221 cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct tggtaaacgg
    17281 tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga ggaactattt
    17341 ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt tttttgtata
    17401 atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg ttttggaaga
    17461 gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga tatatacatt
    17521 tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg aactatttta
    17581 tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata tatataaaag
    17641 aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg ctttattgag
    17701 ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta ttgattttat
    17761 tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga ttttagatag
    17821 tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg ttttttccag
    17881 cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg tgacttggca
    17941 attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga atggtacgat
    18001 gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat aagtttcgga
    18061 ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg gttatcatga
    18121 tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa caaaatcaaa
    18181 aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg ctgaatgata
    18241 aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa aagatcgaaa
    18301 atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta tacgaacagc
    18361 aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc tacgcaaatt
    18421 atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat tatgaatctt
    18481 ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta gacgcctttt
    18541 atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt attggttttc
    18601 aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga ctctctttat
    18661 acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa atagctaaag
    18721 aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat tcacaattgc
    18781 cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct gatgattatc
    18841 gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa aatcttgccc
    18901 cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt tataaaacat
    18961 tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa acagcagcag
    19021 ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgagg gaactttcaa
    19081 tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc tacaaattag
    19141 aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat ccacactttt
    19201 tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt gaagaattag
    19261 cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa gagaaaacga
    19321 tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat caaatgcgtt
    19381 atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag atcgaagtac
    19441 ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt gattttactc
    19501 gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta aggatcatca
    19561 tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa gaaaaactgt
    19621 ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat gaacgtttac
    19681 gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca ggtggactga
    19741 ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg tagatgatga
    19801 atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag gctttcaaat
    19861 agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc gacagattga
    19921 tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta ttgaagcggc
    19981 tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag aattcgatta
    20041 tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg tgaataagtt
    20101 cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa atgaacaaaa
    20161 aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg aagaattgga
    20221 tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata ggatagtatt
    20281 gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag aacggcaaca
    20341 aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt tgttggaggc
    20401 agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc aggaatggct
    20461 gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat tccaacaagc
    20521 gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg ttttttacgc
    20581 agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac gcgtgctgca
    20641 aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac agtttcagtt
    20701 agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta tggagattca
    20761 acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa aaatccatca
    20821 ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa agcatcagag
    20881 acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc agcattatca
    20941 agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca tgtatctagg
    21001 acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc atttacgtat
    21061 ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga tagcaagtga
    21121 gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg tcggcagatc
    21181 ccctaaagag taccgagaaa acagtggaga atcataaaag aaaaagccaa tgtaatgaaa
    21241 taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac ttgaggcaag
    21301 gcagcttttt catgacctct cgataaacgg agttcaaaag ccagtttctc ggtattaagc
    21361 caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttaat actcgaaact
    21421 aaacagcttt ttcacgacct ctccactaaa cggtgttcaa tcagctgacc ctcggtatta
    21481 gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt tgtctctcta atactcaggt
    21541 cagaacgctg atttcacaac ctctcaataa acggtgttca aaagcttgca tctgcggtaa
    21601 taagaccaac caaacgaaaa atatgaggat ctatttttgc ttggctgttc ttattacttg
    21661 atgcaggaca gctttttcac aacctcttgt taattatagt ttgataagga aaaaggcaaa
    21721 tcttttgctt gatatttctt agggtgaggg atgatcccta attggtaaga gatcgtgccg
    21781 cctttcagca aatcttgatg ggtaaaatat aatttcgtat attctctgtc catccgtttg
    21841 attttatgga taaattgttg ctgcggccga ttaggcgttg cttcaatcgt caattccttt
    21901 ccatttgaca aaagcaaaac agccttgtca attaatggca taccgataac atattcaccg
    21961 gttccaggcg tgacaggata aaatcccaga ctattgaaga tataccaagc ggccatactt
    22021 ccattgtcct catctccagg gtaacccatt ggagaatcgt cgaaggtctg agtcatcaac
    22081 tgtttaagca gtggctgtgc catttcaggt tgtcctatat aactgaacaa gtaaggatag
    22141 tgaaaacttg gctggtttga aatagctaat tgtccaaatt ctatcgcggc catctcactc
    22201 atttcgtgga tctcaaaacc atatcctcct gggtcaaacg ttgggggctg attgcacagc
    22261 tcaatgagtt tttctttata tctctcactg ccaccatatt ttccgatcag tcctgaaaaa
    22321 tcgtgataaa cagcaaagct gctttgccaa gcacttcctt ctgcataatc ttgtccccaa
    22381 cgattagctg aaaatggttc tctgaacttg ccatctttat cttttgaacg cataaatcct
    22441 gactcatagt caaagatatt ttgatagttc aatgattgct gctgatattt cttcgtcatt
    22501 tcctcttctt ctagtacctt ggcaacttgg ctaatacaaa aatcgctata agcatagtct
    22561 aatgtgtgat tgactgattc gtggtagtga ttaggaacat agccatatgt caagtaatct
    22621 tctgttcctt ggcgtccata atttggatgt ttgctttgaa ccgtagcacc tttttccatt
    22681 gccgttagaa attcaggcat caagtctcgt cggatatctt ttacagcagc atctgcaatg
    22741 accgcatcga tcaatgtccc aggcataagt ccacgttcat caggagacag ccattttggc
    22801 aaatagccac tttctctata gctgttcaaa aaaccttcca gcatttcttc gtatttttct
    22861 acagcaatca aagaataaag aggataaacc gttttatacg tatcccaaaa cccattattt
    22921 gtatagagaa caccaggttt tatagatcga ctagtcgtat catagtggat tttttgccca
    22981 ctaggatcaa tttcataaaa tgtttgtgga aataaaaaca cccgatataa attatgataa
    23041 aacgtccgca attgttgttg atcatggtgt gtgagctgta tacgatcaaa ataggactgc
    23101 cattgttctt gactgtgagc aaggtaagtc tctttgcttt ggttttgttc tagttccagg
    23161 ttataagctg cttgttccga gctgataaaa gatgtcccaa atcggatagt ttgttttttt
    23221 gtctgttcaa aatacaacgg gatcgagtcg ctatttttgt tggaaacagc taactcatca
    23281 attgttaaag gatattcaaa tctcaatata aaataaaaag aaaaatcagg atcttctgaa
    23341 ccagcaaaat gtgtgatttt tccttccact gtgtaaggat ctataacaaa gagctgatgg
    23401 cgtcccggaa aggataaaag taaaccgctg tcatttttgc tgtaatcaat agataagatt
    23461 ccgccataca tgctaggtat caatgtagaa cagatgccat aacgtaattg acagatcgac
    23521 agatgagtcg ggcaaaatat actttcttct gggcggtaag aactttgagc atggaaaagg
    23581 gtgttctcct gtagttttcc attgatcggc gtaaacagca tatgactgaa atcacccatc
    23641 caaggacttg gttgatgggt aagacggtag ccttgaaaaa cgcggtcatc aggatgaaac
    23701 caccagctgc cgttttgatc cgtagtttgc ggtgcaaaat aattcatccc aaaaggaacg
    23761 ccagtataag gctggcagtt cccattagag aaatttggct gattcgctgt tgcatgtcta
    23821 gtatctattt gaccgatatt cattttattt ctcctttgtt gattgtcttt tgatgaatga
    23881 aactggaaat ttactatcgg atggtcgatc ttttgtctct aaccaagtga ccaaagcatc
    23941 tcccgcaagc ttacctaact gttcaaaatc ttgtgcgatg gttgtcagtg gcgggtcaac
    24001 aagagctgct gcttggatat catcaaatcc gataattgaa aaatcttcag ggatatgata
    24061 accatgctgt ctcaagagat tcatggtctg gatagcgaca acatcgtttt cacaaacaaa
    24121 agcagttact tcatacttag tgatataaga aagcaattca tctaatgtcg cgcttttgtc
    24181 atccagtgaa gtgtggaaag tcaaaccagc tttgtccaat gcttcaaggt aaccaaggta
    24241 acgctgtcga acggattgtg gatgagtctg cgtaccaaac aaataagcga tttttgaatg
    24301 tccctgttcg atcaaataag aggcagcaag cgcaccgcct tggaaattat cggaaagaac
    24361 ggtaggaaaa ggtaactcat agattttttt atctaatatg acgacaggga attctttcaa
    24421 tgaaagctca aaaagtaagt caagatgttg ttctgtatca agagcataat aaatcaatcc
    24481 gtcaaattca ttgattagct cttttgctgt tttattttgt agaaagtcaa gcgtcgtcat
    24541 catgacatca atctgtttat cttgcatcac agggttcaat cccttgctaa aatcaccaac
    24601 agacaaatca gcgacatatg gaagaagaaa cagaatccgt cgtgtttttg taggagaagc
    24661 aagaatctgt tccggagaag cggatttaac gaaactgcct ttcccgcgaa cgcgataaat
    24721 aagtcctaat tgctctaatt cagtcaatgc acgtttggaa gtgattctgc ttactttata
    24781 ggtacgtgaa agttcttttt cagttggtag ttggctgtct acaggtaatt caccagttaa
    24841 aatagccgtt tgcaaatcat taaatattgt ctggtacaat ggttgagtca tataaacacc
    24901 tcttaatgat atatcatatt caaataatac cataatggat gaaaaagtaa ataaaaagat
    24961 attttttgaa agcgtagaca taattggctg tatgaagtat aatctttaaa gatatatcaa
    25021 atagaaagaa ggttttatta gtggtttata aagatatacc aaaatcggtg cagaaattta
    25081 tggaagatat cacacaaaaa tgtggggata tccatgcgga ctgggcaaag aattttacag
    25141 cagcatttgc caatactctg ttgacaacag tcaaaaagca aaatgacgga accacctttt
    25201 tattgacggg agacattcca gcgatgtggt taagagactc tactgcacaa gttcgtcctt
    25261 atttagtgat tgcaaaagaa gatgaggaat tagctgacat gatcagcggg ttagtcaaaa
    25321 agcaattttt ttatatcaat atcgatccat atgcgaatgc cttcaacgaa gaagctaacg
    25381 gtgctggcca ccaaacagat catacaaaaa tgaacgattg gatctgggaa agaaaatatg
    25441 aaattgattc tttatgctac ccaatacagt tggcctatct tctttacaaa aatacaggaa
    25501 aaactgatca attcaatcaa tcttttattg aaggtatcaa aaaaatcttg gacgtgttca
    25561 aaacagagca agatcatgaa cactctcctt atacatttgt acgagacacg acaagaaaag
    25621 aagatacgct gatcaatgac gggcgaggtg caaaaacagc ttttacagga atgacatggt
    25681 ctggtttccg tcctagcgat gatgcttgcc gttatggata tctggtacca tccaatatgt
    25741 ttgcggtggt cgtgttaggt tatgtggaag aaatttttca aacgatactc gatgataaag
    25801 aaattgtaca gcaaacacag aaattgaaga aagagatcca agaggggatt cagaatcacg
    25861 gacgaacggt taatcaaaaa gggcaaacga tttatgcata tgaagtagat ggaatgggaa
    25921 acgccacagt catggatgac agtaatgtgc ccaatctgat cgccgcacct tacttgggat
    25981 attgttctga acaagaggaa gattatctgc aaacaagaag aacattgttg agtaaagaaa
    26041 atccatattt ctatgaagga aagtacgcaa aaggaattgg atcctcccat acgccagaaa
    26101 aatacgtttg gccgattgca ttagctatgg aaggaatgac aacgtcagac aaatctgaaa
    26161 aagagcggat attgaattta cttgtagcaa ctgacgcagg aacacacctt atgcacgaag
    26221 gattcgatgt gaacaatcct gaaaattata cgagagaatg gttttcttgg gcaaatatga
    26281 tgttttgcga attagtcatg gattactttg atattagagt agaaaagtag gaggtatcgt
    26341 aaatgaaaaa gaaagtatat atcatttctc atagccactg ggatcgtgaa tggtacatgg
    26401 cttacgaaca gcatcacatg cgactgattg aattgataga cgatctacta gagctatttg
    26461 aagttgatcc gtccttcaac agttttcatt tggatgggca aacgattatt ttagatgact
    26521 atcttcaagt aaggccagaa aaaagacagg caatccaaca agcaatcaat gaaggaaaat
    26581 tgagaattgg accattttat atcttgcaag atgattttct tatcagctcg gaatcgaacg
    26641 tgagaaatat gctgattggc atggaagaaa gccgtaaatg gggaacccct gtcatgttag
    26701 gctattttcc cgatacattt ggaaatatgg gacagacgcc tcaattaatg aaacaagctg
    26761 gtatttcagc agcagcgttt ggccgtggtg taaaaccaat tggctttgat aatcaagttt
    26821 tggaagcaga aaactattct tcccaatact cagagatgtg gtggaaaggc cctgatcaaa
    26881 cagcaatctt tggtctgcta tttgccaatt ggtatagcaa cgggaatgag attccagtag
    26941 aaaaagaagc agctcttgcc ttttggaagc aaaaattagc ggatgcagaa caatatgcct
    27001 ctacgaacca cttattgatg atgaatggtg ttgatcatca accggtacaa aaagatatat
    27061 caaaagcaat ccatctcgca aatgaactgt ttccagatta cgagtttatt cattctaatt
    27121 ttactgacta tctggaagca gttcaaaaag atgtgccaga agacttaggc tcagtagaag
    27181 gagaattgac aagtcaagaa acggatggct ggtatacttt agccaataca gcatctgctc
    27241 gggtttactt aaaacaatgg aataccaagg tccagcgaca gctagaaaat attacagaac
    27301 cacttgcgac gatggcttat gaagttagtg gaaactatcc ccatgatcaa ttagattatg
    27361 cttggaagac attaatgcaa aatcatcctc acgacagtat ttgcggttgt tcagtcgatt
    27421 ctgtccatcg ggaaatgata cctcgttttg aaaaagcaga tgaagttgga aagtatcttg
    27481 cacaagattc cttagaacag ctgaccgcag caatcgatac gaccggattc ccaaaagaca
    27541 gctttccatt tgtcattgta aatactgcag gaatggataa aactggtgaa gcagaaataa
    27601 ccatcgaatt agaaagaaaa agattcgctg aaggaatacc tgaacaacta tatcaagagt
    27661 tagaaaattt gcctaaacgt aagtatcatg tagaaacaaa aagtggggcc actataccag
    27721 ctattttatc tgaagaaact gtccaatttg gttatgattt gccgaaagac cgtttccgtg
    27781 tgccatatat ggcaagaatg atcaaagtga ctttaccatt agaaaacatg ccagcatttt
    27841 cttgggaaac attcgcgctt gtagaagggg aagcgaaagc agaagaaaag gaaacaatga
    27901 ttcatcagag tggacgaatc atcgaaaacg gccctctaca tttgacgatt gaaaaaaatg
    27961 gtaccattac aatggaagat cgcaaaaaca aacgaaagtt gaataatctg catatttttg
    28021 aagatatcgg cgatatagga aatgagtata tctttaaaca acctttctgt gatcagccaa
    28081 tcctatccag taacaaagaa aactctgaag taaaagtatt agtagataca ccggaaatag
    28141 caaaaataag tattctacag gaaatggaaa tccctgtttc tgcagacgag cgtttagaaa
    28201 aagaacagca gatggttgtg gaatttcgtt atcgaaaagc agaacgatcc aaagaaaaac
    28261 gaatccttca aataaaaaca atcatgacaa tcagaaaaga tagtaaaaaa atcgactttg
    28321 aaacgactct cgacaatcag atgaaagatc atcgcttacg tgtcttgttt cctacaaagt
    28381 tacatgtgga acatcacgaa gcagatagta tttttgaagt ggtgaagcgc ccaaaccatg
    28441 tgtctaaaag ttgggaaaac cccacgaatc cgcaacatca gcaagcattt gtcaatattc
    28501 atgatgaaga gtacggtgta acagtcggaa actttggttt gaatgaatat gaagtaacag
    28561 aagacggaca aatcgcagtg acgctacttc gcagtgtggg agaactaggc gattggggat
    28621 attttccaac accagaagca cagtgtctag gtgagcatcg gttcaattac agcattgaac
    28681 tacatggacc agaagaaaaa ttctctactt atttgcatgc atacgcagca cagattcctt
    28741 tttccactca acaaatcaaa catcatgaag gaacattgat ttcaaaacaa caatatttaa
    28801 cgataaaaag tgaaacgttt gcaattacag ctttgaaacg cagcaagttt agtgataaag
    28861 tagtagtaag aggtttcaat atgtctagtc atttagaaaa attggaaata actaaagaca
    28921 atggaaaaac tgtcatattg aatctgcttg aagaaccaac gaagcaagcg gttgtcccaa
    28981 taatccaacc ttatgagatt cggacaatcg gattcgagga ggaaaacgaa tgtatggcgg
    29041 aatagaagca ggcggaacaa aatttgtgtg tgcggtttca gatggtggag aaattttaga
    29101 aaaattaagt atccctacca caacaccaga agaaacattg gatcaggtat ttgaattttt
    29161 tgatcgttat gaattgaagg gaatcgggat tggctcattc ggcccgattg gtatcgacaa
    29221 aacaaaagat aattacggat acgttttggc aactcccaaa aaagggtggg cacaattcga
    29281 ttttcttggc acaatcaagg aacgctacaa tattcctgct gcttggacaa cggatgtcaa
    29341 tgctgctgca tatggggaat tgaagcaagg agcagcacaa ggaaaaaata gttgtattta
    29401 ccttaccgtg ggaacaggaa tcggtgcagg tgtcgtgatc aatgaagaaa ttttttcagg
    29461 gatcgcacat cctgaaatgg gacatatttg ggtgaaacgc catccagatg accattatga
    29521 aggtacttgt ccttatcata aagattgttt agaaggatta gcagcaggac cgtctattga
    29581 ggcacgcaca gggatcaaag ggcaagatct gccgcaagat catcctgttt gggctattca
    29641 ggcttattat attgctcaag cactgatcaa ctatacgttg acgttagcac ctgaaaagat
    29701 tattttaggc ggtggggtaa tgaatcagga tcatctcctt caaaaaatcc gtcagcaatt
    29761 tgttgaattg atgggtggtt atatggaaac acctcaagta agtgaatata tcgttcgttg
    29821 gggactgccg aatgaaagtg ggattatcgg tagtttgctg ttagcagaac acgaacatca
    29881 aaagtaagca gacaaattac acaaaaagaa cagagtctta tcattatctg ctgataagac
    29941 tctgttcttt tttatttcta ttcccataat ccaggtaaag ctttaccgac tattttttct
    30001 tgagtaaatg gatggatcat gtgcagttct aatgcatgga gcatcagtcg ttcagctgtt
    30061 ctattttggt agagagggtc tccaacgatc ggatgtccct tatgagctaa atgaactctg
    30121 atctggtgtg tacgtccggt ttccaaaaca caatagactt gtgtttgttc agaaagtact
    30181 ttgttgacag tgacatgtgt caaggcgctc tgaccatttt tatcatctac gacccttttt
    30241 ctccgatcat ggcgatctcg tccaatttta ttggtgatgg tcatatcttt ggcaatttct
    30301 ccccatacga tggcttgata tctccgatag atcatttttt gttccaacat tcttccaagt
    30361 atcggcaaga caaatggatt ttttgcaaat aaaacggcac cgcttgtttc tttgtccaag
    30421 cggtggacaa cgtaaggtcg ttgctgttta gtgtcgagat aagcagctaa atgattgagt
    30481 aaggtactgt tttctgtcgg ttcatttgga tgagtcttca ttcctgcagg cttatccact
    30541 ataatcaaat gctcatcttc atataaaaca cggatgtgct tcgcatctcc taattgaact
    30601 tcttgataat tataatctgt ttcttcgaat aacaatgtga tctggtcatt gccctttact
    30661 tcatgatgga acaaagcagg ttgttgattg atccaaacgt ttttacgtat acgcaaaaag
    30721 tgtcgaacct ttctaggaac taaccattcc ttttcaagca agtcgcgaat tgttgtttca
    30781 ggatggttca taggtaaagt gatcgtgatt ttcacaagtg tactcctttc agtgcaattg
    30841 cagactaatt gtaccatttt taaaacatat ttaagaaaaa acttacattt ttctgttcat
    30901 tcttatgtta ctctacttgc actaacgtaa aaaaacatat tgaactacaa cttaagcagg
    30961 aagaaaacac tctttcacta tggtaaaata agacgattga atcaaataga caggagaaga
    31021 agcaaatgga ttttcaaagc attttagaaa agataaaagc tgctttgatc agtttttggc
    31081 gttggatcag accatatctc ggacaattcc atcgatggcg aaagcgcatc tggaaaaaat
    31141 atcatgtgaa caaattgatt ttgctgattg gtttgatctg tgtcttagtg acaagtatct
    31201 acctctttat tttagcaaaa caagcaaatg tcgaaacgtt aaaatcagga ttaagccaat
    31261 cgacagtcat ttatgataag aaaaacgaag aagctggcac actgtacgcc caaaaaggaa
    31321 catatgtaga attaaacgcg atttctccct atatccaaga tgcagtaatc tcgacagaag
    31381 ataggaattt ttacgagcat cacgggtttg atatcaaagg gattgcccgt gcagcagtcc
    31441 gaatggtctt gtctggcgga acatcagggg gcggaggaag tacgatcact cagcagcttg
    31501 ccaaaaatgc gtatctgaca ttagaccaaa cgtttgatcg aaaagcgaag gaattatttt
    31561 tggcaatcga gatcgaaaag aagtacagca aagaagaaat cttgaccatg tatcttaaca
    31621 atgcctattt tggcaacggg gtatggggtg tccaagacgc ttccagaaag tattttggag
    31681 tagatgcaac gaatgttacg ctgtctgagg ctgctacttt ggcaggaatg ctgaaaggtc
    31741 ctggtattta taatccgatc gatcacccaa aaaatgcgaa tgatcggaaa aacacagtac
    31801 taagtgtaat ggaagaaaac ggaaaaatca caaaagaaca agaacaagca gaaagccaga
    31861 cagatatcag cgcgtatctt aatgacacat acgataattc agattctggt tatcgttatc
    31921 cttattattt tgatgcagtg atcgatgaag cagttaacga gtatggtctt gatgaagaag
    31981 atgtcatgaa taaaggctat aaaatctata cagctttaga tcaggattat caaaagcaat
    32041 tagaagctac ttatcaaaac agtgcattat tcccagcaaa tgcttcagat ggtgccatgg
    32101 tccaatctgc ttcagtagct ttagatccga atacaggtgg tgtacaagca ctagtaggac
    32161 gaagaggaga tcatgtcttt agaggattca gctttgctac acagatgcgt cgttcaccag
    32221 gatcaacgat caaaccatta agcgtatata caccagcact ggaagctgga tacaagcctt
    32281 ccagtatctt gaaagacgaa ccgcaaagtt attacgatgc gcataatttt gacgggacat
    32341 accaaggaga agtaccgatg tatcaagctg ttgcacaaag tttgaatttg cctgctgtat
    32401 ggcttttgca tgagatcggt ttacaaaaag gatatgataa agcagaagaa ttcggcctgc
    32461 ctttagcaaa atctgataag tattatggct tagcccttgg tggactggaa aaaggagtat
    32521 ctccattgat catggcgggt gcttacagtg catttgccaa tgatggtcag atgtatacgc
    32581 cgcatcttat cacgaaaatc gttgattcga ctggagcggt gatcgtcgat aacacaaaca
    32641 aaaaaccaaa acaggtcata tcaaaggaaa cagccgatga aatgaccagc atgcttcttg
    32701 gtacattttc aaatggaaca gcggctgctg cgaatcctgc aggttatacg atagcaggga
    32761 aaacaggaac cacagaaact aatttcgatg ccacaaaagc aaatgaccaa tggatgatcg
    32821 gttacacgcc agacgtagtg atttcgacat ggatgggtta cgaagaatcc agcaagctgc
    32881 actatttgga agggacaagt ggaaatgttg tcgggaaagt cttcaaatca gcggcagaag
    32941 gcatccttcc gtatacttca aaaacaggat ttagtgtagc agatgcctat gccacaggag
    33001 gacaagtggt agctgcagat caagttggaa acacaggaaa ttctaatgga gagtctggaa
    33061 attggcaaga taacttgaat caatatggtg aacaagcaaa agaagggctg aaaaacttcg
    33121 gcgatatggt gaaagaaggc gttcagggaa ttggtgaagc ggctaaagac ctctggcgga
    33181 aatatcaagg agagtagccc ctttgaaaac gaataacccc atgttacaat agagagtaac
    33241 tactaaaaga aaagagggca taacatgtca aacatctacg atagcgcgaa tcaaatcgaa
    33301 cgtgagatcc gtgaattacc agaattcaaa gcgttgaaag aagcgtacga gaaagtacaa
    33361 gcaaacgaag aagcacacaa actgttcaaa gacttccaag cgatgcaaat cgaattacaa
    33421 gaaaaacaaa tgagtggaca agagttcagc gacgaagatg caatgaaagc acaagaaatg
    33481 gctgtgaaaa tccaatcaga agaagtaatc aatgacttga tgcaaaaaga gcaaggcttc
    33541 agtacaatca tcaatgactt gaaccgtatc atcatgacac cagttcgcga cctttacagc
    33601 gaataaaact attttatgaa acaacagagg gtgtgaaaga gacgttcggc cttgagcgat
    33661 gacaaaattg cttcttattg ccgataggct gtctcttgaa caccatttac caagaggtcg
    33721 tgagcctacc gtttaactct gagttacaag gaacaatttc aaaaaacagc ttctcatgtt
    33781 tgatgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt attcagaaat
    33841 aggagctacg acaaaagtct tgtcatagct cctatttttt gtcattcatt tttcaagcgg
    33901 tagaaatagc ctttttgagc taacagtttt tcgtaagtac ctatttcaac gatttttcct
    33961 tcatcgatca caacgatttt atctgcctct ttgatcgttg aaagccgatg agccacaata
    34021 aaagtagtgg gtgtttctgt caggtaattc agggcttgct ggattttttt ctctgactgg
    34081 ttatctaaag ctgaggttgc ttcatctagc aaaatgattt ttggctgacg tatcaacgct
    34141 cgtgcaatgg agatacattg cttttggcca ccagacagct tgttgccatg ttcgcctact
    34201 aaggtatcca gaccatcagg aagctggtca atcagtgaag aaagttgcgc agcttcgatg
    34261 acttcatcta aacgttcttt tgatacgttt ttcaagccat aagtgatgtt ctctttgatc
    34321 gaagcggaga acaacaaagt ggtttgagga acaacggcta aataatttcg cacacttcgc
    34381 atatccagtt catccaaagg ctgatcatct agaaaaattt ttcctgaagt cggctggata
    34441 aatccaatca ataagttaag gagtgtagat tttccagagc cagatggccc aacaaaagca
    34501 attgtttctc cagctttcac ctctagagaa aaatgatcga taatcggttt gagtgagtcc
    34561 ggataaagaa aagaaacatc ctcaaaacga aaatttccat ccaaatgttg gatttttttc
    34621 ttaccgtgat aagtttctac atcgccagca atcaacagtt cgccaataga agtgatagat
    34681 tccatgcctt ttgaaatcgt aggaatcagc gtgatcattc cagaaacgga attgacgatc
    34741 gtcgtgaaat acgtctgata catcacgaca tcgccgggtt ggatgcgccc ttgtaaagct
    34801 aatatcccag tgaaaatcaa acaaatcagc tgaaatatct gaaaactagc ccagctgact
    34861 gatccgaaaa cagattgcag gatatctaag tggaatcctt tttcatagat ttgagtaaaa
    34921 tgcttattta agcgagttgt ttcgtgtttt tctaacccat gagctcgagc aatcggtgct
    34981 aattcgatca cttcgatcac ttttgcagaa gtctcttcca tttcttgcct gaagtctgag
    35041 ttggactgtc tgatcctttt acggaaaaag agagtaacga atgcggcgag aggaacagtc
    35101 aataagaaaa acatgaaaac aacaggactt ttataaagcg tgatacctaa agctacgaat
    35161 aaattgacag cgatatttaa tccgctgaca aatacttgag aggacaacgt ttggatagct
    35221 tctacatcac ggatgatctt tgactggatt cgtccagatt gcaattcttt ttgataagga
    35281 atagatagtt cttggatttt cttgataagg gtcgctctta gtccagcttc tacttgacgg
    35341 atcgaagtgc tatggtattt tacatgaaaa taattcgtca atatattttg accaatcaga
    35401 caagccatca aaaaagcgtt gaaataaatg gcctgcaact gagaagaatc tttcgatgaa
    35461 gctgcattga ttacattggc aatagcgatc ggcattaacc aagatggaag atgtttgata
    35521 ataaaaaaga gtcctgacat ggcaaaacgc caataatttc cctgataaag atgcagcaat
    35581 gtctgaaaag catttgcatg atatttttga taacttttct gtattcgttc ctccataatc
    35641 ttctccttaa caatcttggt aaataaactg caagtgttct attgcttcag gaacataaat
    35701 ctcaggagta gcttcaagag tggcgtacat tagaggacgc tcatatttga tataatgcag
    35761 taccgggcgg aaatctaaac agcccttgcc aacaggtacc atgcggatct ttccattttc
    35821 catgatatag tctttgagat gaagcgctgt aatataacca tgtaaattgt ccaaggcacc
    35881 tcggatcact ttttcctgat cagcatgatt ttctttatgc atcaaattcg cacaatctaa
    35941 aatgattttt aaatttggtg aagctacttc atcgattaaa cgtttagcta actgatagtt
    36001 atagattgga tgattgatcc ctgcttcaac agcaacagtt acgccaaaat tttctgcatg
    36061 agcgactaat tcaatgatag aatttctggc gatctgataa gcttcttcag taaaattttc
    36121 ttttgtgtaa ccgacagaga cactaccggt ttccgtagca atcatcttgg cttgataggc
    36181 agagcacaga gcaatttgct gtttgaaagt tgccaatgct tgtgcacgaa cttcttgatt
    36241 tttgctggat aggttgatat agcatcctaa aatggaaagt tcgatattgt ttttttggaa
    36301 gtattcacca aaataggcag cactcccaag tgtcatgtca gttaatttaa gttgttcagg
    36361 aaatgctttt tgtggtgcaa attggatatg ggacaagtga agctgtgata ctttttgaac
    36421 gatttcttca cgatttcgaa catctaaatc gtgggcacgg atgcctaaat ttaatggaaa
    36481 catctcaaac tcctttttgt aaataattag caagaggcag acgggattct tttatcaatt
    36541 caacaattat tcgggcaact ttatcagcgc cttctggact aaagtgggta ttgtctttta
    36601 gtccttctgg gtaattttta caagtatcag gcataagatg caggtagaat ttttttgtct
    36661 cttcttttga gaaggtctgg aacaattctt gtgatcttga gaacacatct aaaagaggaa
    36721 tatggtgttt ctcagcaaat gcacgcatcg cttttggata gtctccaagt atatctggat
    36781 tcaatgttcc gttttctaag taatctcgtc gcgtcacaga tgtcagtaag accatttgga
    36841 cgttcgcttt ttttgcgcat tcaaaaaata tctgcagatt ttttaaatag ctaccgaagg
    36901 gaagcgtacc tctttcttct tggattttct ggtcgttatg tccaaattgt acaaataaga
    36961 aatcattcgg ctgaaactgt gaagctagac gggctaacct tccttcctgg ataaatgatt
    37021 tggtgctgcg tccattcttt gcttggttat tgatctggat ctcgggtgaa aaataagcag
    37081 agattttttc tccccaacct gtctcgggac gcttgttttc atctttttct gccgcagtag
    37141 aatcaccggc aatatggata gtaggcatgt tatctcttct ttcataaagg tttataggca
    37201 atgaagcaga acagcaagag aagaagacgg agcaa
//
LOCUS       DACDTH010000023        36104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDTH010000023 DACDTH010000000
VERSION     DACDTH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.023"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(147..773)
                     /locus_tag="IWK54_001210"
     CDS             complement(147..773)
                     /locus_tag="IWK54_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593341.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1068..1403)
                     /locus_tag="IWK54_001211"
     CDS             complement(1068..1403)
                     /locus_tag="IWK54_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9593342.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1390..1674)
                     /locus_tag="IWK54_001212"
     CDS             complement(1390..1674)
                     /locus_tag="IWK54_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9593343.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1730..3253)
                     /locus_tag="IWK54_001213"
     CDS             complement(1730..3253)
                     /locus_tag="IWK54_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9593344.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3246..4421)
                     /locus_tag="IWK54_001214"
     CDS             complement(3246..4421)
                     /locus_tag="IWK54_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9593345.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4425..4610)
                     /locus_tag="IWK54_001215"
     CDS             complement(4425..4610)
                     /locus_tag="IWK54_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593346.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4576..6270)
                     /locus_tag="IWK54_001216"
     CDS             complement(4576..6270)
                     /locus_tag="IWK54_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9593347.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6267..6740)
                     /locus_tag="IWK54_001217"
     CDS             complement(6267..6740)
                     /locus_tag="IWK54_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP9593348.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6809..6964)
                     /locus_tag="IWK54_001218"
     CDS             complement(6809..6964)
                     /locus_tag="IWK54_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP9593349.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7098..7478)
                     /locus_tag="IWK54_001219"
     CDS             complement(7098..7478)
                     /locus_tag="IWK54_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9593350.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7482..7697)
                     /locus_tag="IWK54_001220"
     CDS             complement(7482..7697)
                     /locus_tag="IWK54_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593351.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7700..8107)
                     /locus_tag="IWK54_001221"
     CDS             complement(7700..8107)
                     /locus_tag="IWK54_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP9593352.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8373..9848)
                     /locus_tag="IWK54_001222"
     CDS             complement(8373..9848)
                     /locus_tag="IWK54_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP9593353.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9838..10686)
                     /locus_tag="IWK54_001223"
     CDS             complement(9838..10686)
                     /locus_tag="IWK54_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP9593354.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10723..11085)
                     /locus_tag="IWK54_001224"
     CDS             complement(10723..11085)
                     /locus_tag="IWK54_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593355.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11385..12009)
                     /locus_tag="IWK54_001225"
                     /pseudo
     CDS             complement(11385..12009)
                     /locus_tag="IWK54_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12006..12317)
                     /locus_tag="IWK54_001226"
     CDS             complement(12006..12317)
                     /locus_tag="IWK54_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593356.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12361..12654)
                     /locus_tag="IWK54_001227"
     CDS             complement(12361..12654)
                     /locus_tag="IWK54_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593357.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12848..13495
                     /locus_tag="IWK54_001228"
     CDS             12848..13495
                     /locus_tag="IWK54_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9593358.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13556..14701
                     /locus_tag="IWK54_001229"
     CDS             13556..14701
                     /locus_tag="IWK54_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9593359.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14782..15087)
                     /locus_tag="IWK54_001230"
     CDS             complement(14782..15087)
                     /locus_tag="IWK54_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9593360.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15112..15543)
                     /gene="ruvX"
                     /locus_tag="IWK54_001231"
     CDS             complement(15112..15543)
                     /gene="ruvX"
                     /locus_tag="IWK54_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9593361.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15540..15806)
                     /locus_tag="IWK54_001232"
     CDS             complement(15540..15806)
                     /locus_tag="IWK54_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9593362.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16061..16786)
                     /locus_tag="IWK54_001233"
     CDS             complement(16061..16786)
                     /locus_tag="IWK54_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9593363.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17147..17217)
                     /locus_tag="IWK54_001234"
     tRNA            complement(17147..17217)
                     /locus_tag="IWK54_001234"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17183..17185),aa:Gly,seq:ccc)
     gene            17334..18086
                     /locus_tag="IWK54_001235"
     CDS             17334..18086
                     /locus_tag="IWK54_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9593364.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18102..18581
                     /locus_tag="IWK54_001236"
     CDS             18102..18581
                     /locus_tag="IWK54_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9593365.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(18832..20412)
                     /locus_tag="IWK54_001237"
     CDS             complement(18832..20412)
                     /locus_tag="IWK54_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9593366.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20405..21148)
                     /locus_tag="IWK54_001238"
     CDS             complement(20405..21148)
                     /locus_tag="IWK54_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9593367.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21324..22100)
                     /locus_tag="IWK54_001239"
     CDS             complement(21324..22100)
                     /locus_tag="IWK54_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9593368.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22348..23184)
                     /locus_tag="IWK54_001240"
     CDS             complement(22348..23184)
                     /locus_tag="IWK54_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9593369.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(23195..24124)
                     /locus_tag="IWK54_001241"
     CDS             complement(23195..24124)
                     /locus_tag="IWK54_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9593370.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            24316..24669
                     /locus_tag="IWK54_001242"
     CDS             24316..24669
                     /locus_tag="IWK54_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9593371.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24743..25399)
                     /locus_tag="IWK54_001243"
     CDS             complement(24743..25399)
                     /locus_tag="IWK54_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9593372.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            25625..27559
                     /locus_tag="IWK54_001244"
     CDS             25625..27559
                     /locus_tag="IWK54_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9593373.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(27645..28868)
                     /locus_tag="IWK54_001245"
     CDS             complement(27645..28868)
                     /locus_tag="IWK54_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9593374.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(28862..30760)
                     /locus_tag="IWK54_001246"
     CDS             complement(28862..30760)
                     /locus_tag="IWK54_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9593375.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(30754..31872)
                     /locus_tag="IWK54_001247"
     CDS             complement(30754..31872)
                     /locus_tag="IWK54_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9593376.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(31955..32788)
                     /locus_tag="IWK54_001248"
     CDS             complement(31955..32788)
                     /locus_tag="IWK54_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9593377.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(32781..33698)
                     /locus_tag="IWK54_001249"
     CDS             complement(32781..33698)
                     /locus_tag="IWK54_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9593378.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(33925..35049)
                     /locus_tag="IWK54_001250"
     CDS             complement(33925..35049)
                     /locus_tag="IWK54_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9593379.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            35232..>36104
                     /locus_tag="IWK54_001251"
     CDS             35232..>36104
                     /locus_tag="IWK54_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9593380.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTG"
BASE COUNT        10906 a         7255 c         5851 g        12092 t
ORIGIN      
        1 gaaacccata tcaaagaact ataaaggtta tcaaaacagc gtttatgact aaatgaatta
       61 gagttatcta tcactatttt tcaagcgatt taaaccaaat ggcatacaat atggcataca
      121 aaaacttcaa taagccgtgg gggtttttaa atttcttctt gttctttaaa atcaatgagt
      181 ggagctacat aatttgaatc cactttttca aacgtcactt tttgcatcga cgaatgtttt
      241 ctttcccaag ataacgtaaa tagatttgac tctggactta taaagtctac ttcaccttct
      301 agtattactt tcttatgcaa atattcctct aataaatata gacttgctaa agcatacaac
      361 acattttcca aatttgcttc cttataattt gaagaacgac catgttttac tctgttatat
      421 attttccacc aatctggtgt atcttcaggc cacccatctc caaaaggttt tagtgaagtc
      481 aatgttaaag aattcataac cctaacacca ttatccaaac acattaattc gctagattta
      541 ataacagctt taaaatcagt tatgctttta ttgctactgc tatcaaatcc gcaaagttct
      601 ttgaaaataa tctcacactc cgaaccaatc gctagtagta gtttttgata ttcaatagag
      661 aatgttttga aattattctc atcgagcatc acaaaatctg tggtattaat aaactcattt
      721 tctaaatgta gataatactt ccaataaaaa tttgtaaact ctgctttgtc cattaaatca
      781 cctcttttga attataacaa aaaaaacacg tccacaacag acgtgttctt tgatttctac
      841 acaatcagct tgccaaaacc atccaatcag tcctagcatg cctgtattaa aattgaacgt
      901 tcatcatcta tcatctttct tagtccagca tgtttgcaac cacacctaca attttcagtc
      961 caacaataga tttcgactag ctaagtcttg caaaattcag gcagctaacc cttcatttac
     1021 taccttaatt atactaaata cccacaacgt ttacaagtgt aatcattcta agaaatctgt
     1081 ttagctatga tcgttgtata atctttaaac gccgttcctt cattgattga aataatttta
     1141 taactcttgt ttttccaagc tactttcata tcgtttgtta gttcataatc ttgatcgtat
     1201 cgaataataa acgtcaacgt tccttcaaga atggtaccta cactagcaag cacgtctttt
     1261 agcatttgtg aacgcacatt agcccaacat tcaaatacag tttcttcact atccaccatc
     1321 tgcccatctt catcttttac ccgttttgtc tttttaaact tgactctctc agtcaattca
     1381 tttacattct taatgattgg cataataagc acctctcatt tgttgtacta atgcttgtac
     1441 cgttactggt atccgttcgc tgctggcttc ttgacggttc aaataccaat gttgagtaag
     1501 taatgacacc gccaaatcaa attgctgata attatccata tctttatccg ttgcgctact
     1561 atcaatagca ctttttatat attcgcctgc tgtatcaatc aattgttgaa taaagtcatc
     1621 atcaatagtg tgatcgatcc gcatactttt ttttatgcta tccaaaatta ccattttatc
     1681 tctcctaatg aaaaaaaggc agtgggataa accgccgccc aatatttttc tatacttttg
     1741 gtgttacaac tggcgtaaac tcaatataca ctgaagcatt ttgatcaatt ttcttataat
     1801 cgttgcgaac gatcactgca agtccttgtg agtagtaatc gaatttttcc cattgagtcg
     1861 ttacttgatt tctacgagca acaaagatag attgtgcaat atcccccata atcataggga
     1921 atgttccagc tttaggattg gcaaacaatg tatctgcaat caatactact ggcataccga
     1981 ataatgattt accactaggt gctgtcacat cgggttgtaa aatgtaacgt ccatctgaat
     2041 ctttcaatgt atctaggtgg taatatccgc tttggtttag aattaccatt ttatttaatg
     2101 ctgggtctaa tgccacattg tatagttgtt tcaaatcgtc caacgtagca gccgttttct
     2161 tagtgaatgt ttttaataaa tccatgatat gcttattgtc agtgttttct accaatttcg
     2221 ctaactgatc tttgacctct tgtacaatat tcactgctga atcttccaca acctcatttg
     2281 ataaggcaat cttaccagca cgagtttcta ctttataatc aactgaagta aacatttcag
     2341 cgtcaatatc accaatttca gcaagttcag cttttgttgc taacactgct tgttggttag
     2401 ttgctactgg atatttacct tgtccatttg atactgtttt tactgttgca tattgagcta
     2461 agttataatt tgaacgtttc aaatcaaaga cttcaccgat tacttcttcg ggaacaactg
     2521 ccgctgcatt tacagtagtg actccatcac gcacttcgcc ttgtgaacgg atatattctt
     2581 catacccacg caattctgtt ttttcgttat cgattaaagt tttttccatg ttattactct
     2641 ccttttgtgc tttagaagtg gattctaagc tattgtttgt ttgttttgct tggttgttac
     2701 tcataaacga ttcgtaagaa cgcttattta cttggacatt tgatgaatca taagcgggaa
     2761 cggtcacgac actaatttca ttaagtgctt tgattttatc tattgatcgt tcaattgtgc
     2821 catcttcttt tttgtcgaat gaatctaatc caagcacgaa cccaaaggac atagaatcaa
     2881 caaccccttt tgagatattc tcatacacat catttgaata ggtcgtgtcg gttagctctg
     2941 cttcaaaatg taatcctgta tcatcgacat tcaatttcaa cgtacctgct ttgacactag
     3001 ctagtggctt tgaataatcg tgtccatata ataagaacac atttgataaa tccacctctt
     3061 ttaatgcttc gggtgtaatc acttctacaa agccgcctaa atcttttgac ggttgcccga
     3121 atttcaacgc atagcctgaa atagttttgc cgtctgttgg ttgtttttca ttttctttct
     3181 ctactttggg agaatcggct gttagctcag cttcttctgt cagccgtttt tccttttctt
     3241 cttcattatt caactggtgt aactccttcc tgttctttta attgatattt ctccaacgta
     3301 tctaaatacg taaagttcaa acttgctagt aaacgatcgc caccatccat ggggggtagc
     3361 cccattttcg accgcccctc attaatcgtg agaagtgacc ctttgacctg atccaataca
     3421 ttttttactt ttgtttcagg atcagtctct agtaactgat cggaattaaa gcggatattg
     3481 gtttcaagtt tcgtatctaa ttcactcaca aacacattaa agtaatgaat cagcgtactt
     3541 tggatatact ggagattact ttgaacggtg ctagaatgtt cattttcaac gcctaaacgc
     3601 tcaatcggta caccaaacgc cttggcaatt tgttttgtgt tccaatcatt ggaattgata
     3661 agcttcaaaa catcggtgtt tatttctaat gttttgtagt ccattgtttc atcaagaatg
     3721 atcgttctaa gagcattatc tccactactt gaaccgttgg cttcttcaaa cttttcacga
     3781 attgcgttct ttgctgatcc gtctaaatct gacttatgaa ccttcaaaat acctgaccca
     3841 ctgacaccac gagtaaagaa attgtgcaat gtgcggttgc cagcttgttg aattttcagt
     3901 tcatcacgta acgcataaag tggtggtaat cctgttaaac catcttgggt gaaatatttg
     3961 aagtgcaaaa tatcgctaga cttcacacgt ctttttttat caccaatctg ataagacaac
     4021 gtaccattat ctaattgagt aaccgttaca cttgagttag gtaataagtg aatttcttct
     4081 accttttcac cgttgcgttt aatctctgca aagctattac cgtttagcaa catattgacc
     4141 gccaaagcaa actttaaatg ccaaccatcc atttctgaat taggtttctc gtttagtaac
     4201 ttcaccagtt cattatcagc ttgtggcata ttgtttttga ccaattgaat cggacttgaa
     4261 gcaatatcac tagcaataat tcgaacggct gcaaacacat cactatttct taatgcacga
     4321 acgcttgtaa agctggtggt gtaatcatcc gattggattg ataccacatg ttgtaggaaa
     4381 ggatctcccg ttgtattttg tctcgcttta aaaaatgcca tcttttaacc tcccttctgt
     4441 tctgtattta gcatgaccgc taccccaata agcactatgc ccagcgctaa caagccatag
     4501 tagacatttg ttagtaaggt gatagcaacc aacacacaca ccaatccaag aagtaaaaga
     4561 acggtatgaa tgtaattaga aagaaaattc ttcacttgta taaaattcat tgtctgcttt
     4621 cgctccttcc gttgtggtga agtaatccat tgcaaacaca taggcattga ttaatgcagc
     4681 tattggatca attttgttac tgttctttga tttattaatc tgaatgccgt tgttatccgt
     4741 ttttaaaata gcgttgttca ctgcatgggt gagaatcgta tttttattgt gaataacgtt
     4801 gccttcataa acttgttctc taaaagttcg ggtcggaacg tttagagtaa tcgttccttg
     4861 tcttacttct aacattgggt agttggcttt ttcagcttta ctgataagcg aattagcgtt
     4921 atacgggtca taacaaatcc ccatacattc caaatcattt tcttgaatca aatcttgaat
     4981 aaactgaaat acttggtcat agtccacgat tccactttct aattgagtaa tggaacactc
     5041 accagctttt tctagttctc gataaggcaa accgtcgcgc ttttctttgt cttgaagccc
     5101 atatttggtg gctacaaagc tatgtgaatc acaataaagt tcgccgttat ctagcggtac
     5161 gatccatgaa acgctagtta aatcatctgt tttagataaa tccactccga tataaacggg
     5221 cttgcctgta atatcttgtg gttctacttc ggttgcctgc cagtcttcag caatcatata
     5281 gctgtcctca ctcgcttgtc tccataaatt gaagtttttc accaatacag aattaaggtt
     5341 attttgtttc aaagcaaggt tcacatcatc ttgaatggtt ggaatcatta cttttttgat
     5401 ttcttcactt tcaaaaatcg gattggcttt gatccatttc tcttgatcgt gaatttcttc
     5461 gggatcgtcc aattcccata tcgctataaa gtacctgtcg gcttgttctt cgccttttag
     5521 cacacgatct aacaataagt attcctcgta cataggaacg tttaaatcaa gcccagcggt
     5581 actaataaca cacaataagc cgttcttttg ttgggtcatt cctgacttaa tgacgttgta
     5641 cgttttacga gtttttgctt cgtgccactc gtcaagaatt gcggttgttg gtgcaaaccc
     5701 atctagcgtg ctggtttcac tagccagtgc catagcgaac gaattagagg gcaagtgctg
     5761 aatcttagag ttcataattt tcagttgtgg tcttaaaaac ttactagact tgaccacgtt
     5821 tctaagtgaa ttagacagca tatcatagcc caatttagct tgtttcaaag cgttacttac
     5881 aaacaatact tgccttgctt ctgctggttc ttgttccata atcaacgcat tggcagccat
     5941 gccacttgcg atatacgttt tcccattctt acggctcata ctggtaaacg ctcgattgaa
     6001 tcgcctgtaa ttcccttctt tagtacgcca gccataaaga ctgcctagaa tgaatttttg
     6061 aaatcctaac atcttgattg ctttaccgtc cgtagatggt agcaactcaa tgaatttaat
     6121 cgctttggtg gcttgttctt catcgaacac ataagggaag ctatctgact tagaacgctc
     6181 caaatcgctt atatgacgct cacacgcttg gataattttc ttgggtgcaa tcagcttacc
     6241 tgataggact ttctcaatgt aatcattcat gaattcatca actctttgaa cgggtcttcg
     6301 ggttcatcat ttttaacctt gttcaaagct agttttgatc gtgattctaa ggtcatacct
     6361 agtgaggtgg cttgtttttt caaatcactc atagcttgtg attgaacccg aaccgctgga
     6421 ttggctttct ttactccgtt ttcgcatgtg ataaacgtac ctgattttct tatctcggct
     6481 tcggctgttt tcattcgagc aaaggctaaa caataggcta ccaatgaacc ataatctgct
     6541 tcactcaatg gataatcttt ttttaatgtt ggtacgatac gattccattc acttctagcg
     6601 ctcgctgcca tccagtcagg gggcgtgata tttattaatg gttgctgctt gaacaattct
     6661 tctctagcgt ccacacggat tgctttttct tcacttgata tattgccttt tgtatcgtgt
     6721 aatattttag ggtgtcccat gttgtcctcc tttcagacaa aagaaaaagg aacgtaagca
     6781 attaagctca cattccttcg tttttcgatc agaatttttc tttatctaat ttatctatat
     6841 aaatgatttt tccgttctgc atttgaatgg tcacttgacc aaaatgaggt aactcaatgg
     6901 tgcgctgttt ccctgtttta tcagtaataa caattgtatt tttcaataaa tctcctactt
     6961 tcattatata ctttttcact tgtgattaca agtgtaatcg ttatttgtaa ttacccttat
     7021 gccttttcac aacggttatt tgtaccaaaa tgagtaggca tcgattgtca gtaagtttca
     7081 cactatgccc ccttaactca aagggtactc ttttctcgtc tttgtctttc attggctgtc
     7141 ttggaattat ggtgtgcatg gcataaagaa cgaaaattag tttcatctaa ccgtctattc
     7201 caatcatctt ttagttcaat tttgtgatct acaacatcag ctttccttat caagccttct
     7261 tctaggcaat cctcacatac tggatgattt aaccgataca agtatgaagc ctttcgccat
     7321 gttctacttt gatagaattg aaaatattta ccttcgcttt gctttctttc ttcacgctta
     7381 attgccgttt gctttgcttt atgcttccca caatacttct gcctataatc aatcaatgtt
     7441 ttacatccag catgattaca ttgtttttta actgccatag attaccaacc aaagcgatct
     7501 cgttcgtcag aatcatcttg ttcccatttt gatttgtatt caatataagg cgaaactatc
     7561 ttcaagcacg ttccgtcttt aaaattaact ttagtgattt tatcttcctc aaaactaata
     7621 aagtctacat cttcttgagt gtttgaatac tcaatgtgtt tctcaccatc gaagtatcta
     7681 atactttcca tatgcataat tattctacct ccaataaatt agttatcaat tcatcatctg
     7741 ttagtagcaa agccaagaac ttatcatgga tgtttaaaaa caaattatcc tcattcgatg
     7801 taagctctcg ttgtctcgct cgatcatata ggttaagcag acaattaaat tcatccatta
     7861 gctcacctgt atcatcatag ttgccactat ctaccaatga tcccactact agcgattgac
     7921 accacgctac tgactcctgc agccaatcag gttcttcttc cattatcttt aatacttctg
     7981 gatcaaatgt ggaataatct tctgttacca atcccacact aaatgccagc gtgttcatta
     8041 gaaaccgatc catttcatct gtatattgaa agttcggtgc tttgtttgtt gaaataatat
     8101 tgagcattta ctttcctcct tgattcgtaa cacgtggtaa cacctacgta acaggtaccc
     8161 gttaccactc aatcccaacg gggacaaggg ttctagcgtt ttggtaacag gtaacgggta
     8221 aaatccagta acattattcc ccagcatatt agctaactat ttcaaaagtt ttaaggaaat
     8281 aaggtgttac cccgttacca aattgctgta tcccttgggg cagtaagggt aaactagtaa
     8341 cgggctaggt gttactgacc cgttactacc cgttactttt tgtactttcc agctcgattt
     8401 ttattttcat aggcatttga cttattgtaa aatcctcgtc gtttatttct ttttggcagt
     8461 tgcttccgtt cccaaccagg aatactgcta ataatcaagg caatcttttt agtttctgca
     8521 tctgatcgac cattcaataa atcctttgct tgtttatcaa aaacagcttc caaaatatca
     8581 gcggtgagta cgctatcaac taaataaaac tcttgttgca tagtagctcc attatagaaa
     8641 tgctgtaaat ttttctcatt acatttactg tcaggatagt tctgataata agaacgtctc
     8701 gcccacgctg gagcttcttc ccatttatac gggatttcca tttccaaata tttagtgatt
     8761 gcttctttaa ttggattctc catcttggca tcctcttgat agtcttttgc aatttctaag
     8821 acttctttat cttcatcggg actaaaatag attcgttgtc ctttatcaaa aagcaccttt
     8881 gcttcagcta acacttgaag aaaatattca tcctttgttt taaacggatt catctttggc
     8941 ttattcttac aaggcaaagg ataaaaacgg cggtttcctg tatcatcttt caaatactgc
     9001 ccatcattcg acgttccaat aaatacacat tgccttgccc aagctatgac attccgacca
     9061 taaggaggtc taattttatc gatcttggca ctgatgaagt tttttatctg ttcaatttta
     9121 gttttgttca ttgaagataa ttctccaagt tcaataatta cattgccttg aagctgcata
     9181 taatcatctt tcttttcacc tagcgaatct aatgaatcgg tgaaaaaatc atccgtgtaa
     9241 agtaatgctg gaacggtact cttaccaatc ccttgcggtc catccaaaac tggaacgatc
     9301 tcaaacttta ttcctggttt atagatacgt gcgactgatc cgactatcca acgttttgtt
     9361 acttctcgtg tgtaaaggtt gtcgtctacc cctaaaaggt caataaacag tgtttccagt
     9421 ctaggctgtt gatcccattt aactttttcg atacgttctt ttactgggtt gaaaacattc
     9481 tttctagcca catttactac tgctttgtgt attttgttgt catcaaaaac tacattgtag
     9541 ttttcttcaa tatatgccgt taaggcatcc tcaaaactgt cttcccattc tccagcggta
     9601 cgattgatcc aaggagaatc ttttaatagg tgaatgtatc cgctaaattc gttataagca
     9661 attgttccag ctaaattgct atcattggtt aaaatcaatt ctgcattcac aacactgtta
     9721 cttttaatct gatcgtcttt attgcatctt aatcgtgaca tccaggtttt caattcaatg
     9781 atattgtctt cacgctcgct ggcttcggga aagtctaaaa tactatctaa tttatgctta
     9841 tgagccatga gctttccccc tcaaatcttt tttaatcatc gatcgaataa tttgattcac
     9901 ttctttatct tttagcggtg gttcattgta cgcatttgct gtataaatca attctgctaa
     9961 agtttgatta tctacaccgt gccatagtaa ccaaccgcat aacttagtta aatgatcgtt
    10021 tctactacct tcgccagcgc cttgaacaat catatctaat ttttttgctg tccctgtttt
    10081 tgctacgggt cggtattgag tctttatttc ttgcggtttt tttagcagta ggttcactag
    10141 ccaatcggga gctggtgcaa tattatttag agatacgttt tcaataggtt catatgctct
    10201 cccaacactt cttgaaataa tcatagttgg cgctattgga actcctttag tgataatatc
    10261 aatcccgctt aaacttttac ctttatcatc acgcccgaag atatcagcaa atgcacctgt
    10321 ttcttgttgg ataattacac catcaggaat tttaaagaag aaatggattc ctttacctgg
    10381 tgtgatttcc gtataagtag aaggaaatgg atcatacatt gaagcaagtt ttttataatt
    10441 ctcaacgccg tttcgtgttt cagaatgttg atctaggtca actaccacca tttgatgatc
    10501 ttcaagcatt aacccaatat ttctagttgc atcttccgtc caccattcat taatttgatt
    10561 agaatcgtta gttgcatcat tccaaccact agagcctttt attggaattt tagttccagg
    10621 agataaggga taaattgacc atcccgactt ttgtaattcc gttgctgctt gataattatt
    10681 tgccaataaa cctctcctcc tttttcaaaa tactggtata aatcattcag acacctccat
    10741 gagcttcaac tgctcaagtt cgtatttcaa cccatcccga acaaattcag caccttttaa
    10801 ttgattctga tactttaccc atgtattaaa tgttttcacc cttgcttctt gcatatcagt
    10861 aatgtttaac catgcggtta aaatctgatt ttttagctcg ttgatttctc gacaaattat
    10921 tttgtgttct gcatgttttt tgagtacata caaaactgtt agtttttcat tgaatgaatt
    10981 tccaaagtag gttttcccga tattattttc tagttcagcg atcccttgat taatttcctc
    11041 taattcaagg ctatcgagtt ggtttaatag ttttgttttg tacatctact tatgccctcc
    11101 agttcttaat tgatagtcgt actcgtcata agcagtgaag agatataacg ctaatcctgc
    11161 aaagatagct atttttgcaa ttaatggtaa taagccgaaa attccgccga ttaaaatgct
    11221 tcctataatt aagtttcgtt tcataatggt tccctccgat tttttaatat ggtataatcg
    11281 gaacaaagaa tatcgggttt tatcccaatt aagttgcctt tatcctgtcc gccaagactg
    11341 ttatggataa ggctttttct ttgttctcga tcatgttaat gctcctaagt tgtctgcaat
    11401 tcagcagaac gattataaat tttctcactc actgaaaact gcaacccata tttttcttta
    11461 acagtcatta attctactgt ctcatctaaa ataggttcac gatcaaccaa catttcagga
    11521 gtcatatctt tcttttttac cattttggaa tgaccataaa tagttgatac agctttattg
    11581 gctatcacat tcgctttaat gtagtctact ggttttggtt cagtaatagc gtggcttaat
    11641 ttagtcattg cttctttttg gtgttctttg tctagcatgc ggaatacttg aaagccttca
    11701 agtcctgtgg actggcgtag ttctttgatg atgtcgaaaa tccagtcttg aaatgcctcg
    11761 gcttcttttt tacggctttt gaatgccatg cggtaaatat tttttttcgt tgatgatgtt
    11821 tacatcttgt gttctgccca atgaatcgat gaccttacta gtagtaaccc catctttagg
    11881 caaagagtga atggctctag taacctgttt aagacctaac gcttccgcaa catctttcgc
    11941 cactgcccac cattcatcat taatatcgac aaatcttata atgtgtccgt tccaaatttc
    12001 agttttcatt caacgtcctc cagttcaagc cctagcaagc tcgctaacaa ctcaacttgc
    12061 tgatacaaaa tttcttgata ctcttcaata gaaagtcgtt ctttaaactc tgcttgtact
    12121 aaatcgttca agccacgcaa tactccgctt accgttggtt cttgtggtag caatttttga
    12181 tagtccattt gttcgtacaa acattcaaac gtttcttcta gcgctttgcc ttccaagttt
    12241 gctaattctg ccgctgtgtc ttctgtaaga attgttgata atactttttc gattgcttta
    12301 ttttgtgtcg attccattat tatttcctcc tattaatccc tttgccccca cggcttgcgt
    12361 ttatttttgg tattcttcca taaagcgatc acattcatct tgtgaaattc ttacgactcc
    12421 atcaactata atcaacttta aaccgttagg aacatatttc tgcatcatag tattgtatga
    12481 aacgttcata tacttagcag cttgtgcctt gttcatatat tttggaaact ggtacttttt
    12541 ttctaataat gaaagtaacg ttccttgagt ttgctctact tgttgaacaa ctcgatcgtc
    12601 aataatttta aaagctggat catcaagcgt gagtttgtca atttttaatt ccatatttat
    12661 cccccctcgt tagcatacat ttctatacaa agattttaaa aaaatgcaag aggctcttga
    12721 atacatttct atacctctta taaactcaat ataccatcat accttttcat ttgcaagaat
    12781 tttaacatac ttttttgaac ttttatgttt cacacatatt taaatcgtga tatagtagag
    12841 aaaatatatg aatggtggtg aaaatgtggc tagaaaaaaa taccccgaag cagtggagac
    12901 aggacaaaga attagactgg ctagaattga aaagggactc tcaatggaac aacttggagg
    12961 taaattatcc ccaccggcca gtaaaggtgc cgttagtaat tgggaaaatg gctataatct
    13021 tcccaataac accaggctta aacaactttc aaaggtatta gatgtatcca ctacctatct
    13081 attaaacgga tcttatacat taagagatat gcatatgatg cccgaaatta agcgaaatgt
    13141 gcttgaaaag caattgaaaa aaactattga aaaaaataac tctataattg attctaatct
    13201 ttctaatgct atcaataaca tcgaagaaga taacctgact gtctcacaga agatttattt
    13261 aacaaacgca ataaaatatc ttaaaacagg atctcgaatt gaatttcctg acgggagtac
    13321 cccaattgct tatctcgcag ctcttctaaa tacgctttta cgatatgact cattaattaa
    13381 tgattcagaa aaaaatacag atgagaaaaa agagtatcaa gaattttatg aaaatgatct
    13441 tctaaaagag ttcacagctc ttgttactga gctaaataaa cactattcaa aataataata
    13501 aactgcctcc tccaaccgcc cccacggctt gctacactat ggagggaaaa ctattatggc
    13561 aacattcaag caatatgaaa ctaaaaaagg taaattttgg ctctatgaag cctatcttgg
    13621 aatgaacaaa atgactggca aacctgataa ggttcgtaga cgaggttaca aaacaaaaaa
    13681 agaagcccag cttgccttgt cacgcttgca agtggactac gataaaaacg gtattaaaaa
    13741 agcgaacaac gaaacgttta aagaagttta tgacttgtgg ctagaaacat ataaaaccac
    13801 tgtccgagaa gccaccttca tgaaaactga aatcaagttt agaaagtgga ttcttccaaa
    13861 atatggaaat cttcgtgtga atgaagtcac ggtaaaaaaa gcccaacaaa ttgtaaatag
    13921 ctgggctgaa actactgacc aatacaaggt acttcattca acttctaagc gtatttttag
    13981 ctacgctatc aatcttggga ttattgagac taatccacta gacaacgtat taatgcctaa
    14041 aagaaccacc actaagcata aagaaaaggt caaagtttac agtaaagaac aactgatcac
    14101 cttgtttgat tacctaaaca gcaaacctga aacttatcga aacgactttg ataaaacgtt
    14161 actacgcttt ctcttttata gcggggttag gatcagtgaa gcccttgcat tgaattggtc
    14221 agacattgat tttcaagaaa atacggtcac catcaataaa acactaagcc aatccaaaaa
    14281 tggctataaa atcagtgatc ctaaaactga ttgtagtgct ggggttcttc cactagatga
    14341 aagaacaatt tctatattaa aaaaatggca gattaatcag cggaaatata tgctgacact
    14401 tggtgtaact gatccgacta tgattttttg tggtatctat aaagaactaa tcacacacca
    14461 cgctatttat gcaagattga ttactatttg tgaaaaagct atggttccat ttctcggtgt
    14521 gcatgtagca agacacactc atgcttcaat gctcttggac gctggagcta ctatggtaga
    14581 agttcaaact agattacggc atacaaaaat tagtcagacg atcgattgtt acggtcactt
    14641 ggcaaaagaa acgaaagaaa agactgttga aaaattagta caacacttaa atattgacta
    14701 gatggcatac aatatggcat acagtaaaaa ataaaaaaag gagaaaccct atcatatcaa
    14761 ggtttctcct agctttatag tctattcttc ttcttccgcc atgaatgtat tgaatacttc
    14821 ttcaatcatg tcccattctg cgtctgtttc gatttgttct aactcgcctt ctgttccatc
    14881 ttcgttttcg atgtatgcat aagcttgtaa ttctacatct tcatcttctg aaacaccagc
    14941 aggatataaa agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg ttagtaggat
    15001 ttcatataat gtttcatttc cttggtcatc caccaatgtg atgtgttcgt gtccttcatg
    15061 gtcgtgggaa tggttatgtt ctgtcataag attacctctt ttcttttatt attaaaataa
    15121 ttgcttacct gagccgtcta aataattttg taagatcatc acagctgcta atttatcgat
    15181 gacttttttt cgtttggctc ttgaagtatt tgcctgctct actagcatac gttctgcctg
    15241 aacagtggtt aatcgttcat cttgataatc aactggcaag gagaatagtt ctttgatctt
    15301 ttctccgtat gacatggaag cttctgccct tggtccgatg gaattgttca tatttttagg
    15361 taatccaact acaaaccggt ctacttcata ttgtgctact aattccttca accgatcaaa
    15421 gccaaactct tcttctgctt cattgatccg aatgatctct actccttgag ctgtccagcc
    15481 aaaggggtca ctgatagcaa cacctactgt cttagaaccg acatccaagc ccattactct
    15541 cataaattca ctccatgatt tgagaggtag tccttgacaa ttacttccat gatctcatcg
    15601 cgttcgtgac ggcgaatcaa atttcgagca tcacgataac gaggaatgta ggccgggtct
    15661 ccagaaagca agtagccgac aatctgattg attggattgt agcctttttc ttccaaagcc
    15721 cgataaacaa tcgctaatgt ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa
    15781 cgtacggttt catctgtaaa acccatgatt ctacacctct ttcccgcaag gtaattccag
    15841 cttaccttct acaaccttat tttactcgaa gaaagggaaa actacaaaca aatattccaa
    15901 acttccgaat aaattaagaa acactatttg tatgtttttt tccttttttc tgtagcttta
    15961 tcaaaagtac tcttttcata aagtatacgc tatatttctt tgaatgtatg tttttacaca
    16021 attttttcat aatattcata aaaaattcga atagctgcta ctaatttttt gtttttgttg
    16081 cttgtcctgt ctcataattg atcgtgtagc catcttgaac attgtagata aagacattga
    16141 attcaatttt tttgtcttca atgctttctg cttccaattg cactcccctg gctaccagtt
    16201 cattcccctc aaaatgagga gtgacacggt agcgtacatg atgatttgtt tctttgatat
    16261 atgccgcaat atcattttcg tgagcaagca tgtatggagc attcaacgaa cgtgtccctg
    16321 tcatcaagtt tttaatgttg ttattttgac cagtcagctg gaaaccaatt aaatggctgc
    16381 gattatataa ccattgcccg tcacttaatt tcttttgttt ccaacctgtc gggtcaatat
    16441 acaaaggctc tctcttttca gtagggaaca aatcttttcc aagcatagca ttggcttgtc
    16501 ctactctgtt caatttatca atatttgaat agctttccca tgttcctttg gaaaggctaa
    16561 gatcctcttt tgtaaattcc ggctgattcc cattgatttc tatttcttga tgttttccat
    16621 catattctgg cacatccgct acttgagaag cttgtggtgt ggaatcaaaa atagttgtaa
    16681 tctgctgaaa gatatctgaa tctttttgtg tggtggaaac accgaatgcc cctgcaatca
    16741 cagcagcgac gatccccacg atcgccttgg ttgttttttt gctcatttct ttactccttt
    16801 actaacagct ccctaaatct taacatatgt tcccctataa ataaatacta gaaagaaata
    16861 gaaacagcaa aaaataaaac ctgttttaag atggcttaaa aatacaaaaa agagacggat
    16921 tcctcgtctc taatcagtca ccactctatc tgtaaggtat ttattattat tcaaaaattt
    16981 tcttcatact caagacaata cactgatttc acaaccgctt ggtaaacggt gcgcaaaagc
    17041 ttgcatctgc ggtaataagt ccatccaagt taaaaacata agaagttgtt tttgcttggc
    17101 tgttcttatt acttgttgca gagcagcttt ttcacaacct attttaagcg ggtgacggga
    17161 atcgaacccg cgacatcagc ttgggaagct gggattttac cactaaacta cacccgcaat
    17221 acaaaaacag tatacgcctc ttttattcct tcctcaagtt tttttattct ttacgcttct
    17281 ggtatgtttt gtagtaaaat aaaactaatc aatcaattat tggagaagat gctatgacgg
    17341 gaaaagaatt agatctgttg ctggacactt gtttactcgc tgggaaaatc atgatggaaa
    17401 gcaatgcgga gatgtatcgg gtagaagata cgatgagtcg aattgcttta gcttctggaa
    17461 atttccgttt ggtcagctat gtcactcaaa ccggtctctt tattggtctt gatcgtacct
    17521 cgaccatccg aatggaacaa attaccaacc gttcgattaa tttagaaaaa gtagtaaaag
    17581 taaatgatct ttcacgtaaa tatgttgctt cagagctaac attagaagaa ctttactgcg
    17641 aactgcaagc aatcgaccga gaccgttctt tctttcctgt ctggttgcaa atcgttagcg
    17701 cggcagtgat cagtggaaca attatggtgt tatttggcgg aaagatgacc gatttacctg
    17761 tgactttagt cattggagga ttgggatacg cagcttatct atatagtttg aaatttttta
    17821 gaatcaaatt tttatcggaa tttttatctt ctttattgat tggcatagcc gctatctaca
    17881 gcgtacgttt tggcttagga agcaatcagg atctaatcat tatcggatgt gtgatgcctt
    17941 tagttcctgg agttcaaatt acgaacgcta tccgtgattt gctagctgga cattatgtct
    18001 caggagtttc tcgcggaaca gaagcgatga tgactgctac gatgatcggt tttgcgattg
    18061 cttttatttt ccagcttttt tattaggaaa ggaacacaag tatggggacg atatttattc
    18121 agttttcttt tagtttttta gcgactgcgg catttgccgt tattacaaat gtacctaggc
    18181 gttcattgat ttgttgtggt ctttctggtg cttttggctg gatgatctac tggatctgtg
    18241 ttcaaatagg cggatctgct gcttttggtt cattgcttgg ttctttagga gtagcattca
    18301 tcagcgatct tttctctaaa cgtttgaaaa tgcccgtaac gattttcaat attcccggga
    18361 tggttcctct agtccctggt ggtttagctt atcaagcggt tcgaaatctc gttactgaat
    18421 cctatcgaga agcggctcat tatgcggtcc aagcaattat gattgctgga gcaatcgctt
    18481 taggtcttgt attatcagaa gtctttaacc ataacatccg taatttcaaa gtaaagcgtg
    18541 acgcaatcat gatgaaacga aaaaaacggc tttttaaata aacgaagtgt aacaaagaaa
    18601 aggttgtgaa agagacgttc agccttgagc gatgacaaaa tatctcacaa acattgcgac
    18661 aagtaaccgc aggagcaata agaagtaatt atgagtatta aaaaaagttt tgtaaaaata
    18721 gcttctcaca tttttacata aaattgactt aatactgaga aactgacttt tgaacaccct
    18781 ctatctgtaa aaaaggggct gcgacatgat ttttgtcacg gcccctcttc tttattcaaa
    18841 ttttttccaa aacgttcgtt gataataaac gatccataga gcggaaagta ttagaactaa
    18901 accgatcaca ggaatattca ctaaccaaaa tggataaaac aaagccaaca ttccgtataa
    18961 gacgataaga ataatactga tgatcaatga acccatcata gttgcgacta aacccaaact
    19021 tcctgaacct ctgttaaata attggttaat attggtccag ttcagcagca gcaaccgata
    19081 atctctagcg aaataacgta gtgacaataa gtagcagccg aataaagaac caaatagcaa
    19141 gctagggata aaaaacaaag gcagctgaaa agacagtccg cttaataatg cgatcccacc
    19201 agttaaaaaa ctctgcaata tccatccgaa tctgaacttt tcttttaaat attggttcat
    19261 agagattggc aaagaacgga taaacagaaa attttcttga tccaaagaaa taagattgct
    19321 gataaaagaa gtctgattaa ctgttaagaa agccaatgcg atccctacca ggaaaacaac
    19381 accgattaac ttattatcta aatcagttaa ttttatttcg cctcctaggg caaaagcaag
    19441 aataaaaatg attggagtta acaaggaaga agaaagaact tgcataatca aattaggttc
    19501 ttttactaat tgagcattgt aactaaataa aagctgatgc acattttggt tctttttatg
    19561 ggttcttttt attttgaact cactgtttga atcagtcagt tgctctgtaa gctttggtac
    19621 tatcagctgt tttacagcaa ggaaaaatcc cagagccgtg atagctagta ttccccaacc
    19681 caataaacct tgttgagaaa gtggctgact ggcagcatca tagaatggca ataatatcga
    19741 aatcggatgc cgatctgtta actcattttc aatagtcccc gtttgatgat tcatccacat
    19801 gattccagca aatgcgatca gcatcgagct gccaagcaag atactagaaa ctgtttttct
    19861 atgtttttta aacactgctg ttcgagccaa accaaaaaca atcaaactag ccagacaaaa
    19921 aagaataatc aaaaatagag aaaacaaaag gattgagata atgatagtaa gcaaaatccc
    19981 tagatcagct cgccatccgg ttaaaataaa catgactaga agcggaaaaa caaaaggaat
    20041 gatggtcaaa gcaactacca aaatctttgc tgaaaaaacc tgttcttgtc caaaaggcag
    20101 aggcaaaaat acttgcaggt catttccttc aaaaaagata ttataaatga ctgatatact
    20161 ttgagttaaa cctaacatac caaaaagtgc cacgtaataa gtaaagaaac ctggcaattt
    20221 aacaaagttc atagcaaaca tagcaaatcc gtaaatcagt aaaaaaatca gccctgaaaa
    20281 aagattttga gacaaaagat agatagttaa tcgctttccc ttttttcctt tctctcttgc
    20341 tttattcgtg agctgtggat taacatataa aagattaact cgaattaatt ctaataattg
    20401 tcgcttatac atacggtcca tctcctgtcg cttgccttcc agccattttc aaataaatct
    20461 tttctagcgt ttcatcaggt gagccattca ataattcact aactgccccc tgataaatca
    20521 attcaccttt cttcaaaata gccagtttat cgcataactg ttgtgcggta tccaatacat
    20581 gtgtagaaaa aatgactgtt tttccttttg cggcatgagc cttcatcatt tctttcaaat
    20641 caaatgctgc ttgaggatca agtccttgca aaggttcatc caatacccaa atatctggat
    20701 ctggcagcaa cgtgccgatc aaaatcgttt tttgacgcat tccatgtgaa aaacttgcga
    20761 ttgtttcatt ttgatgacta tacatatcaa aaagcgctgt caattctgct aaacgctttt
    20821 cttttttttg tgtgtctaat tcataggcag ctgaaatcaa gtcccaatat tctccagccg
    20881 tcaattgtaa aaaaatatct ggggaatcag gcacataacc gattttttgt ttgatactca
    20941 atcgattttc cgtcaatttc attccatcta ctaaaatcgt tcccgatgat ggttcaataa
    21001 tactaactaa acttttaatc gtagttgatt ttccggcacc attatgtcct aaaaatccaa
    21061 aaatttcccc ttgtcttatc gagagagaca agtcttttaa tgcttgtttt ttcccgtaac
    21121 ttttacttac attgatgaat tcaatcattt ctgtactcct tttgtttgtt attacttttt
    21181 attgtaccct tcgttattgg tggagtaaat gtcaaaaaga gtgcataaca aaaaaggata
    21241 gaaacgacta ggctgctact tgggttgttc tcttctacag gaaaacactc ttctaacagg
    21301 taagatatga gtctttgttt tctttaaaat gattcatttt ctatcttttc ttgcataaaa
    21361 ttctttagtt tatcaatact tctctttccc atctctagtt gttctccatt tgcaaatgtt
    21421 actatttcgt ttactctgct aatagatgta atgttttcag tattaatgat ataagctttt
    21481 aaatctttac ataaatactc atattcgagt gattttttat attttttaat tgtgccgttt
    21541 acgagaatct cttcatcttt caatactagg cctattttat ttttataacc tggcatgctc
    21601 attagataaa ggatttcatc gtaatggata tagtgatcgc tattaccata acggacagtt
    21661 gtccaccttt ttttcgtagc atgttgaaca ggatagtttt ctttttcttt caataagtaa
    21721 ctttggatgc tttttttatt tttttcataa gtgaattctt ttatcaaata accactaggc
    21781 gtaatctttc gattaataac agttattgct ttattcggat gagacgttag ataaacaatt
    21841 ttacactttt cattcttttc tctaagcatt tcgcctagtt cgataccgct ataataggcg
    21901 ttcaaatcga tgtctaggaa aaaaatatct gaggaataga tttcattatt tttaatgaat
    21961 tcaacaaatt gtataacgtt caatccctga acgatctcaa atttccaagg acttaataaa
    22021 attctggatt cttccaaaat ttctactagc attttttcaa aaaccaaact atcatccaaa
    22081 ataaatatac gcgttaccat aaaaagtacc tccactcgat attttactat caaaatcatt
    22141 tcatacaact tttcaacata catgttagga ataaattgta attctcttca caatcttttc
    22201 caacaccttt gaaaccgata tcaaatactt tcaggatgct gcttctaaaa taaatatcaa
    22261 acaaatgaat acatcatgca aaaaaagcca gacaaccgac gtccttgcgt gtcagctgtc
    22321 tgagctatgt ttatttattt tgttctttta aaaagaacga tacacatact gatttttcgg
    22381 agcagagact ttctcagcgt ttgtaacaac tacagatggg atttcacgct tgaatggttg
    22441 ataatagtct gattggatgg ttccttctat tttataccat tcatcatttt taaccgttac
    22501 tccttccggc atatgcgtca acaaaccaaa gactcctgaa tctgccacac aatggataat
    22561 gccgaatcgg aacaagaaga tatctgtatt gctatcgttc ggtgtttggt aaacaaatcc
    22621 ttcatagctg atttttttgc caatgaattc gctagggtaa ttataaatta gctccataac
    22681 ttctaaatag ttttcatctg tgatcttgat agtttgctta tccccatact tttctttcaa
    22741 ctcattcatt tggtctaagt agtctgattt gttgaaataa atactggtat ccggctttaa
    22801 gtattgtgtc tgcatctccg gatcccctac tgattcttta cttactggga aattaaatcc
    22861 tttggcttct acgatcgtag tgtcgagact aactgttgga aacaacgtcc ccacgattac
    22921 tggcaaagct agcagtacat aagcgattcc acgttgataa ggttttgaca acccatggtc
    22981 atgatcgtgc tcgtgtgcct tttcttctgc ttttccgttt ttgacccaca aatacagctg
    23041 aactaaagcc aaaagagcag ataaaatcat tgaaagaata gctaagtagc gataatggac
    23101 attgatatat tgatcgagtt tgccagaaac ttgcaagtac atcatcaatt cgaaataccc
    23161 agataaaatc aaaaaccgca gcatattttc cctcctaaat aaataatgtg aatccaataa
    23221 cgctaagggc aatcagtccg atcagcgata aaataaagcg tcctgtaaaa tatcgtttca
    23281 tcatcagtag atttttgata tcgaccattg ggccaaagac caaaaaggca gctacaggtg
    23341 ctgtgccgaa cagacttaac aaagaagatc ctataaacgc atctgcttct gagcaaagag
    23401 acatcgtaaa ggccaataac agcatgatga taatggctaa aatctttgtt gagccgagcg
    23461 ttagcataat tctagttggt aaataagtct gcatcgcagc tgcgattaat ccgccaatga
    23521 tcaaataacg acctgtatca aaaaattcgt cgattgaatg tgtcaacaca tgcccagttt
    23581 gatggatcag ctgatttttt ggttcattct tctcactttg ttcttcttca tggacatgat
    23641 gatggtgatt ttctgctact ttagacgtga ttttttcttt caatacagcg tctttttgaa
    23701 aataggcaag ccagacacca atcacaaggg caacaaaaaa gctgcctatc acccgccagc
    23761 ttgccatctg ccaagaattg ccaaatgcga taaaagtgga aaaaatcacg attggattga
    23821 tgatcggagc agtcagcata aaagcaaacg ctgtgtgaac tggtacgcct tttttaacaa
    23881 attgatgaac gatcggtacg atgccgcatt cacatgaagg aaagaaaaat cctaagatcg
    23941 aaccagtaaa aatagagaag atacgatttt caggaagcca tctcttcaca cgttctgggg
    24001 tcaaaaatac ttgtaaggcg cctgagataa gacagcctaa taatacgaaa ggtaatgctt
    24061 caataacgat cgataagaaa atcgtactca tctgcaaaac cgaatttgga ataaaagaaa
    24121 acatgagttt cctccttttt atttctagtt cattcgcttt gcaaatatac gcttttattc
    24181 aaaaaaaaca agttgctctg taaattttta cacaactctt attttttacc aaagcacttc
    24241 aattgacttt ttctgttatt cccattaaat taaggtctca tacatatcac ttctatcata
    24301 aaagggagaa taaaaatgaa acaaaccact aacttgactg aagttcaaga catactgcaa
    24361 cagtcatccg ttgccgtcat ttatctttcg atgcctaatt gcagtgtttg tcacgctgtt
    24421 cgtccacgac tagaggaact tctcacacat tatgatattc cggcacttca tttggatgca
    24481 tttgaaacac cagaagtagc tagtaaattt gaagtgctga ctgcgccagt tgtcctgatt
    24541 tttcatcaag gaaaagaagt ctttcggcaa gcacggttca tcgactttaa aaaaatccgt
    24601 tatctactag atgaactgac agcagaagat gattcccttt cttatgaaga aatcttccga
    24661 acaaaataat cgttgtccaa acagaaaaaa gcgattgaac aagtgaaagt tttgccttca
    24721 tttgttcaat cgctttttat ttttattctg ctgtattttc ggaatgcgca tttgcgtcag
    24781 aatgcaagaa atagatcaag gtttgcaatt cattcgtcaa atcaacattt tggatcaata
    24841 catctgacgg agcattcaat cgagcagcgg taaagttcaa gattcctttg attcctgctt
    24901 ccgttaactg attgactact tcttgtgctc tttcagcggg tatcgttaaa attgctactt
    24961 cgatttgttg gatcttgatt tgttctgaca tatcttccat tggataaacg gggataccgt
    25021 ctacgatgcg cccaacgatt tcctcattca catcaaaagc acaactgata cgtatgctat
    25081 tactttgatg gaatttgtat tttagtaaag cacttcccaa gttccctaca ccgatcagcg
    25141 caacatttgt caattgatcg tcgtttaatg tctttgcaaa aaagtgcata agatcttcta
    25201 catcataacc atacccacgt tttcctaatt cgccaaaata agaaaaatct cgacggatcg
    25261 ttgcactatc tacttgaacg gcttcactta attcagttga agacactttt ttctttccag
    25321 cattatttaa aatccttaaa tagcgataat acaaaggcag tcttttagct gtcgcctttg
    25381 gaataatatg atctttcacg aaatgacctc cacgtattgt ctaattacat ctttgatttc
    25441 tccatcatag cagtatttat aggaaaaaat caattccatt gctcagatat tgtgaaattt
    25501 tattctttat tcatttattt atcatttctg caaatctcgc agtataatgg aatttatggt
    25561 agactactaa ttgaaaaata gttttttatt tgaggagaat tcacaaagta gaaaagagga
    25621 ttccatgata ttattacaag ccaatcagat tgctcgccat tttggtgcag aagtcttatt
    25681 tgaaaacata catttagaaa tcagtacagg cgctcgtgtc gctttagttg gcagaaatgg
    25741 cgctggaaaa tcgactttat taaagatcat tgctggaatt gaagcacctg atgaaggaaa
    25801 gatcgctaaa aataaaacag cgactctcgg atacctcgct caggacacag gacttgattc
    25861 caatgaaaca gtttggaacg agatgctgaa agcttttgaa caagttagac aaatggaaga
    25921 gcggatgcgc gagctagaga ttttcattag cgaaggtacg ccagacacac ctgcttatag
    25981 ttcttattta aaagaatacg accggctgca acacgatttt tcagatcgaa acggctatgg
    26041 ttatgaaaat gaaattcgtt ccgtattgca tgggtttaaa ttcgattctt cattttatga
    26101 tcagaaaatc gatactttat caggcggaca gcgcacacgt ttagctttag ctcgcatgct
    26161 tttgcaacac cctgacattt tgattctaga tgaaccaacg aaccatttag atatcgagac
    26221 actttcctgg cttgagaatt atctgcaaag ctatactggt gcattattga ttgtttccca
    26281 tgaccgttat tttttagata aagtagtcaa tgaagtctat gaattaagca gaagaaaaat
    26341 gcgccattac aaaggtaact attcgaaata tctagatttg aaagcagaac agttagcaag
    26401 tgaatggaag gcttatgaaa agcagcaaac agaaatccat aaattagaag acttcgttgc
    26461 tcggaacctt gttcgcgcct ctaccacaaa acgagcgcaa agccgcagaa agacattaga
    26521 aaaaatggac cggctggatc gtcctcaagg ggatgaaaaa caagcaaact ttctattcag
    26581 tatagaaaag ccttctggaa atgtagtctt gcaaatcgaa gatgccgcta tcggatatga
    26641 taatgtcacc ttatctgaac caatcaatct agatatccgt cgtcaagaag cgattgctct
    26701 agtaggtccg aatggtatcg ggaaatcgac gttgctgaaa tcaatcattc accagatccc
    26761 ttttatccgt ggaaaagaaa cgctagggac aaatgtcacc atcggctatt acgatcaagg
    26821 tcaagctgat ctgcatacaa acaagacgat cctagcagaa ttgtgggatg agcatcctac
    26881 cactccagaa aaagagatcc gaagtgcttt aggtgctttc ttattttctg gcgaagatgt
    26941 agaaaaacca atccctctgc taagtggcgg tgaaaaagca cgggttgctt tagccaagtt
    27001 agcgatgaac aaagaaaatt ttttgatttt ggatgaacca acaaaccatt tagatattga
    27061 caacaaagaa gtattagaaa atgcattgat cgattatgac ggcacactgc tttttgtttc
    27121 ccatgaccga tatttcatca atcggatcgc gacaaaagtc gtcgaactct ctgaaaatgg
    27181 cagcaaagtc tatctaggag actatgatta ttatttagag aaaaagcagg aagaagaaga
    27241 aatcgccgaa ttgttggcac aagatgagcc agaatctgct cctgctccca cgaacaaatc
    27301 tgattattac caaagcaaag aacagcaaaa gcttatccgt agccttcaac gaaaaattac
    27361 tcaggttgaa gaagaaatgg cgcggatcga tgaatggatc gatcagttgg aacaagaaat
    27421 ggtcgcgcca gaaaacctgg aagatcacgt aaaactcaat gagctgaacc aagatttaga
    27481 agctgctaga caagatcaag aaacaaaatt gacggaatgg gaagaactaa gcttggaatt
    27541 agaagaatat gaaaattaac gaaaaaacca aaacagaaac cattcatgct gttttggttt
    27601 ttcttttttt attttatcaa acaaaaattc catatttacc tttttcacca ataagaagac
    27661 caattactag tcattctcac taaagcttct aaataatagt aatcacccca aatacaaggt
    27721 gtatttaccc cattattttt gggcttatta taaacagact gtgttaaaaa gccatcttct
    27781 tcttccgttg tagcatatct ttctttcaat tgtcgcataa ttttccaaac agcagactgg
    27841 tataagttct tttctgtttt gtctaataga cctgattcta ttaattctaa taatccacaa
    27901 accatgatca ccatactaga tgtatctttt tcttctctgc tagaatcatc aaagatcaaa
    27961 tcccaataac aaatggtatc ttctggcaac cgattcataa aataattggc taatattttg
    28021 cttaaatcaa aatatttttg ttctttcaaa taacgataac ttaatgcaaa accgtaaatc
    28081 ccccatgctt ggcctctagc ccaacaggaa ttatcactat aaccttgatg cgtacttcca
    28141 tatcttggtt ttccatcttt gacatccata taaaatgtat gataagttga accatcttct
    28201 cttactaaat aatctgctgc attttgaata tgagcctgag cagctttttt atatttttga
    28261 tttccagttt cttcactcgc ccaatacaat aatggaagat tcatgcaaca atccatgatc
    28321 attctacctt gttgtgaagg atcactagga tctccccaag cttgtataac tcctgccttc
    28381 tcccaaaaac gctgaagtaa tgtttctgca gcatcaattg aagcttcctt catttttcga
    28441 tttcccgtta ttttataatc tgctacggtt gataaagaat acaaaaaacc aagatcgtga
    28501 tgattagtga taatttgaca gtcaatcctg tggataaaat ctatatctgc tttttcagct
    28561 gtttgttgaa ataaaggatc acccgtcttc tcaaataaca accaattaat tcctgtccaa
    28621 aatccttctg tccaaccaat attgtctctt aatggataac gtccttttat aggacactcg
    28681 cttgggaatt gatactgact gttactgata cgtttgatta acctctccgt gtgtctgcga
    28741 caacgctcga tctcattatt ccattctttt tccattaatt ctttataaca ttcatttatc
    28801 catttttctg gatgtattat ctcttcaaac atcactttat caagcatttc cccaccagcc
    28861 tttagcataa ttttagtctt tttttattgt ttttattttg gatagttagc ccggttgtcc
    28921 catcaaaatc aattttggga cgattgttcc atacttctaa actataatcc cgatcttgat
    28981 gagcaaccac tcctgttccg agccaatggg ttccttttcc aaatgtaccc tctaatccag
    29041 gaataaaagt gttttcccat gtcatcaagt ttgtattggg caaacccata atagctgtgg
    29101 attttcgttt aatagtatcg tcacttaaac agataatacc tgaaaagcca gcttcactca
    29161 ccctatagtt ttccttgtct tcttctatga ttattccttc tttctcagga gaacattcaa
    29221 tagcaaaccc tgtctcatat agaacatatt ctgtatctaa ttctactttg tgtacgcgaa
    29281 tctgccagcc ttcaaaagga ataagccaag ttctgatttt tgcctgtaaa gtaggttccc
    29341 atgtagaggc aagtacctct ttcttacata caatgtctcg tacattattt cgaacgatga
    29401 actgtccata ggttttgatg ctctcattct taaaactggt cgtgatatat gtgtccgctc
    29461 gctgaatccc tagcattgaa tccattgcca tttctgcttt caattgattc gctcgcggta
    29521 tcgaaaaacc aaattgacta gaataagcaa attttaaata tttttcattg gtcaatgtat
    29581 ggaaatttgg ccctaattgt cccgcattca aaaaaaatgt atgtccatta tcatgttgta
    29641 tgagcaaatt ggcttctgaa agaagtttag ttccttcacg aataggcata ggcatttctt
    29701 tagccgtcca atactcatgg tcttcaggta gcgccaacaa taaaaaaatc ttattcagcc
    29761 ataatggaga taacgtacta ttatagggtt cagtcaatgt caattgaggg taggtataac
    29821 caatattcag gatacctttt tcatcaaata tttctttttg catccaccat cttatattcc
    29881 gattgactaa ccccttgatt tcacctaatt taaaaggcaa aagatcggcc cacaacatag
    29941 ctgaccaaaa gcttgaaact gcaaaacgat aaatcatgct tcgaccaaaa ggcacatttg
    30001 caccatcttc cgcaaaaaaa tgaatatagt ctctagaaaa atcaatagct cggttcttat
    30061 atatctggct aatgggcttt tctggttcta gtacgctata aatcaaacca taataatgaa
    30121 atgcaaaagg attataataa tcttggcgac ctcttgaact gtctcgatac cagccatttt
    30181 gaagataaca attttctata ttatcaaatg cttctttcag tgctgattct ttaactggat
    30241 aaccaaattt ttggataatt gttccgacaa ttatcttaaa aaattgccaa ttaccatctg
    30301 cacattcata ttcaaaaatt tgcgagaacc aatgaataat ctggcttttt tctcctgtat
    30361 ctaatttttt ccataataat tgttcatgat gatataacgc taataacatt gcgggcattt
    30421 ctaccatacg ctggtctctt tcatgtattt ctccccaata atcgggatgg gaaggatctg
    30481 ttccagcttt tattcctgat agaattaatt tcaaccatcg ttcatctaat tggtcaattg
    30541 cgggccccgc tccccataaa acccgagaga atccttccat tcctgcaatt ttatttccat
    30601 atcctacccc ttgatgtata taatcaattt gtgcgccact tgctcgataa aacggctgga
    30661 gtggttgatt tatcttttta aataagtcaa agtaatcctg tctggtcctt aatagtggta
    30721 ctggatgttt cattgtattt tctcctcttt ttactaccaa atagctaatt ccgactgttc
    30781 taatcgaagt aagccttcaa taaaaaaata atcagaatag atcagtggca cattataagt
    30841 cagttctcta tggtatgctt ctgctccgcc tccaatgatc ccatcttcat ccatattcca
    30901 atttgcatag tgcttttctg tcttttggag tatattgata gcagcttcat gatacatttt
    30961 tgatcgtaca gaggaaacat atcctgctaa ttccagaaga ccgcatgcag cgcatactcc
    31021 agcactggta tcaatattcg aatcttttac agcaaaatca atgagaggaa tataaccagt
    31081 tttttcgatt ttctgaataa aatttactgc tatcttttct gctatagcta gatagtacgg
    31141 attttttgtg taacgataac ttaacgcgaa tccatataaa gcccatgcat ttcctctgct
    31201 ccaacaagaa ttctcattgt atccttgccc gccaagtaac cgaatatatt tccctgattc
    31261 tgcatcaaat tctgcgatat gcccggttga tccatctttc ctcactagat acttagctgt
    31321 tgtatctgca tgctgccgag caattttaga gaacctcgga tcaccagttt ccctcgaagc
    31381 ccaatacagt aatggtaaat tcatcatact atcaataatc atccaaccag gtttatggtc
    31441 attccatgct actaaatagt tccctgaaat attatagcgt ccagcaagta ccgtagctgc
    31501 atgtaaccct agttgtcgac tatgtttgtt ttgagactgt tgataatgtg caatagccga
    31561 tggtaagaat aaaaaaccta catcatgatg aacgtctgaa aaatggtaca gggcagattc
    31621 taataattgt tcttgttgta ttgctgcctc tttgaacttt tctcgttttg tcttgtgaaa
    31681 gagctgccat aaaataccac tccaaaaacc attggtccac cagtcgattc ctctgtcctt
    31741 tattacgtct gtatattttc cgttgtaaga aatatatgga attttattcc ccattctgtc
    31801 taattccgca tctatctttt cagaagctac attcaaaatt tttcttgacc attccttcgt
    31861 ctgttcattc atggatatcc cgccattctt ttaattttta tgaatgtgga gttgctccta
    31921 gaacgaacta gatggttaac attcgttatt ttttttactt atttgcagct ccaatgtcat
    31981 tctccttaaa tttagactgc gcccacacat aaatcaagac cattgggatc actgaagttg
    32041 ctaaaattag cataattgcg ccataattaa ttggctcacc tgtactagaa accgtcatat
    32101 ttttgataat taagggaacc gtatatttat ctttatctga aagcatgact aatggaaata
    32161 gatattgatt ccaacttccc ataaagataa gtaaaccact agtaataatc gtaggtgata
    32221 aaatcggtac agcgattttc gtaaaaatca cccactcatt ggcaccatcg attctggcag
    32281 cctccatcag tgctgtagga aattgactgc tatattgtcg catcataaac actgaagttg
    32341 tactggcaat cgaagggatg atcacacctg cataagtatt cgataatcct aaacgactca
    32401 taatgtaaaa taaaggaatc aatgtggtaa aaaaaggcac catcctagat agcataactg
    32461 tattgaacaa cagtttattc cccataaaac gatactttgc taatgcataa cccgccattg
    32521 tagaactggc tgtagctaaa actgtactga ttacagcaat aacaatcgaa ttccaaataa
    32581 tcaagggata attatatctc gccatgatgg cttgccagtt ttttaatagc atatccccaa
    32641 acttgaaatt gattccgcca cttaaaattt cttgattact ttgcgttgaa gctacaatca
    32701 tatataaata tggcatcaaa aatattaagg caccaaacac aataatgacg tacgtaaaaa
    32761 ttctgctttg tatcttttgt ttagtcattt ttatcacctc cgaatttaaa ttgaatagcc
    32821 gataatataa cagatacaaa caccatcgtc caggcaatag cagaggcttt tccaaaatca
    32881 accatttcaa aacttgtttt atataaattg actcctacga ccaaagctga attttctggt
    32941 ccattggaag tcccaaagaa tatatttggg atattgaaat tacccaagcc attgatagtg
    33001 gctaaaactg tggccattaa aatgattggc tttagcatgg ggatcgttaa taaaaagaat
    33061 tgccgaaatt tcgatgctcc atctatttca gcactttcgt atatctcttt agaaatccct
    33121 tgaagaccag ctaaaaataa aatagtgtta tatcctacac taagccaaat catcgtgatg
    33181 atcattgata ttcgtatcca aaaaggattt gttaaccact gtacaccgtt aattcctagc
    33241 gcattcaaaa atccattgaa taacccagtc ggattgaaaa aagtcatgaa tacggaagct
    33301 acagccaccg ttgatgtaat ggttggaata taatagacag ttctaaaaaa tccttttcct
    33361 tttaactgtg ctgaattcaa taaaacagca aagtaaatag ctaatagcat cgttattgga
    33421 attgatcctg ccatgataat caaaacattc gtgtaagatt taataaacaa tggatctcgg
    33481 aagatagcta gataattgtc aaatccaaca aattcaaatg agtagccaat tttcataaaa
    33541 ctaaaagcaa acgagataat gatgggaatc aaaatcgtca caataaagaa aataacaaaa
    33601 ggtgtgacca atccataagc acttttttta tgagacgtat ctgttgcttt tttcttgctg
    33661 ttctttattg tcttgcttgg taacgaagat tgcatcatcg ttaagtcctc ctaatatttt
    33721 ttaatcacca gttaatttta cgccgtactt atgagcaaaa ttttctgctt cttttagata
    33781 tgaattttca aaatcacctg tttgccaata gttatatgca gcggtagtca aataagacgt
    33841 agcatctgaa taagtggctg gtaaaacagt atttgatgta ttttcattga ttttattcaa
    33901 tagttcatag tatttttgtc ctccttagta gtcaaacttt ttctcagctg cttctgtttg
    33961 ataggccaat gagttagatg tagccacgcc cagctccagt gcgttggcta agcattcttc
    34021 atctgtcagc ataaatgtaa gaaattgttg tgccacttgt ggatttttgg catctttagg
    34081 gacataaaaa cttgatcctc ctgatactgc accttttcct ggattttctt ttgaaaaagg
    34141 aatcagtgga gccatgcgcc atttgtcaga tgcatcagga aaattcaaag acatattcgt
    34201 agcataccag ccgccagcaa caaacattgc agattcttga aatgctttct cagcatcttg
    34261 tgcaccgtag aaatgtacaa tatctgagtc gatcatttct ttgattaact ttgcagcatg
    34321 tttcgcttct ttagtattca gacggatatt tccttcttca tcaagcaaag attgattctg
    34381 ctgggctagc atgttgataa acaattcttg gtccccagat tctggcaagg caattccaaa
    34441 actaccagtt ttttctttta atgcttttgc cacctggata tattcttccc aagaactgat
    34501 ttcttcaaaa tctacaccta tttcagcaaa tttttttggt tgataataac aaagtgctgg
    34561 ggtaaaatca ctagcccaag gaataatcgt ttcattactc gtttgttgac gcaaaatatt
    34621 caatttttga tcataaaact gatcgccata tttgtcataa aatccatatt catcagcact
    34681 ataaaaagca tcttgaaatt tttccagcac acctgctgct gaagtgtctg ccatggagat
    34741 aatatccggc atatcttcgt gtgttaccaa ttttggggtg atcatttgta ctgttgctgt
    34801 tcccgaagct acattcacaa tttttaagtc aatatttgct ccagttttgc ggttatagat
    34861 ttttgcagca ttttcaacat agtttgtcag tttgccgttt gcgacatata cttctaatgt
    34921 agtggctttt tttatcattg gatctgcgat gtttgctgtt acttcttcgt tagtttttcc
    34981 acatgcactt aagctgaata atagggtaat gtttagcaaa gctatcacca atttgtatct
    35041 ttttttcatg tctatccctt ctttcttttc aagttcttat ataaaacgct ttcacttatg
    35101 tatattaacg gaattagtaa cacaaatatt tatataaaaa aatattttac ttgtcgtttt
    35161 ggcttttcat ttttgttata atagtcatta attataaata gaaagctttt tatataaaaa
    35221 gagaaaggaa agtggttctt ttgttaacaa acaaccaatc ctccaatctt gtcaaaaaaa
    35281 cactaaccta ttccatagat acccatcttc atttatctgc tgctccctat tttcgactac
    35341 taataaatga aagcgctaat ttatatgctg aatttgcaca taaatctata gaactagata
    35401 atccttccct tttattcatt gataaacagc aagaagtcca aatcttttct actcaaaagg
    35461 aaacacaaac ttttatcatt atccagtttt attttgatga gcaattatta gcacatatta
    35521 cgggacaatc tttttctata gatcaagaaa taacatctat cattactttt ctgaaaaatg
    35581 aacagttcaa aattgattat ctaaaacata gtaagccagc tatttcctat gatcaggaaa
    35641 tcaattcttt tttcatacta tgccaacaga tgagcattca tttgctttcc ttattagtta
    35701 cttattgtct gcaaaaaatt caattgtctt ccttaccaag gcattatcaa caggaacttg
    35761 ctttattgac aataccttca aatgagaaag tcattcctaa aaaaaagcat attctttatt
    35821 cagatgaatt agttgatcca atcatacgat atttagaaga acatctttca gaaaattttt
    35881 tgcttgaaca attagcccaa atttttttta tcagtccagc cagcttaaaa aaacatttta
    35941 gaaaagtgaa tggaaaaagt gttattagct attttaaaga actaaaaatg gaaaaagcaa
    36001 aacgtctcgt gctagaaaat gaattaacct atacagaaat tgcagatata ttaggatttc
    36061 attcgatcca tcatttttct tcagctttta aaaaatatac aggg
//
LOCUS       DACDTH010000024        35576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDTH010000024 DACDTH010000000
VERSION     DACDTH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.024"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(30..830)
                     /locus_tag="IWK54_001252"
     CDS             complement(30..830)
                     /locus_tag="IWK54_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9593381.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(827..1639)
                     /locus_tag="IWK54_001253"
     CDS             complement(827..1639)
                     /locus_tag="IWK54_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9593382.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(1834..2391)
                     /gene="frr"
                     /locus_tag="IWK54_001254"
     CDS             complement(1834..2391)
                     /gene="frr"
                     /locus_tag="IWK54_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9593383.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(2394..3116)
                     /locus_tag="IWK54_001255"
     CDS             complement(2394..3116)
                     /locus_tag="IWK54_001255"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9593384.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(3252..4133)
                     /locus_tag="IWK54_001256"
     CDS             complement(3252..4133)
                     /locus_tag="IWK54_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9593385.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(4232..5014)
                     /gene="rpsB"
                     /locus_tag="IWK54_001257"
     CDS             complement(4232..5014)
                     /gene="rpsB"
                     /locus_tag="IWK54_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9593386.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            5373..5852
                     /locus_tag="IWK54_001258"
     CDS             5373..5852
                     /locus_tag="IWK54_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9593387.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            6069..7160
                     /gene="phnW"
                     /locus_tag="IWK54_001259"
     CDS             6069..7160
                     /gene="phnW"
                     /locus_tag="IWK54_001259"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9593388.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            7153..7281
                     /locus_tag="IWK54_001260"
     CDS             7153..7281
                     /locus_tag="IWK54_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9593389.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            7285..7830
                     /locus_tag="IWK54_001261"
     CDS             7285..7830
                     /locus_tag="IWK54_001261"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9593390.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(8284..9975)
                     /locus_tag="IWK54_001262"
     CDS             complement(8284..9975)
                     /locus_tag="IWK54_001262"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9593391.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(10395..11342)
                     /gene="arcC"
                     /locus_tag="IWK54_001263"
     CDS             complement(10395..11342)
                     /gene="arcC"
                     /locus_tag="IWK54_001263"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9593392.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(11457..12476)
                     /gene="argF"
                     /locus_tag="IWK54_001264"
     CDS             complement(11457..12476)
                     /gene="argF"
                     /locus_tag="IWK54_001264"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9593393.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(12567..13796)
                     /gene="arcA"
                     /locus_tag="IWK54_001265"
     CDS             complement(12567..13796)
                     /gene="arcA"
                     /locus_tag="IWK54_001265"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9593394.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            14257..14958
                     /locus_tag="IWK54_001266"
     CDS             14257..14958
                     /locus_tag="IWK54_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9593395.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(15567..16583)
                     /gene="tsaD"
                     /locus_tag="IWK54_001267"
     CDS             complement(15567..16583)
                     /gene="tsaD"
                     /locus_tag="IWK54_001267"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9593396.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(16580..17044)
                     /gene="rimI"
                     /locus_tag="IWK54_001268"
     CDS             complement(16580..17044)
                     /gene="rimI"
                     /locus_tag="IWK54_001268"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9593397.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(17051..17593)
                     /gene="rimI"
                     /locus_tag="IWK54_001269"
     CDS             complement(17051..17593)
                     /gene="rimI"
                     /locus_tag="IWK54_001269"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9593398.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(17577..18401)
                     /gene="tsaB"
                     /locus_tag="IWK54_001270"
     CDS             complement(17577..18401)
                     /gene="tsaB"
                     /locus_tag="IWK54_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9593399.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(18490..19470)
                     /locus_tag="IWK54_001271"
     CDS             complement(18490..19470)
                     /locus_tag="IWK54_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9593400.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(19494..20978)
                     /gene="galT"
                     /locus_tag="IWK54_001272"
     CDS             complement(19494..20978)
                     /gene="galT"
                     /locus_tag="IWK54_001272"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9593401.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(20990..21979)
                     /gene="galE"
                     /locus_tag="IWK54_001273"
     CDS             complement(20990..21979)
                     /gene="galE"
                     /locus_tag="IWK54_001273"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9593402.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            22227..22394
                     /locus_tag="IWK54_001274"
     CDS             22227..22394
                     /locus_tag="IWK54_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9593403.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(22456..24267)
                     /locus_tag="IWK54_001275"
     CDS             complement(22456..24267)
                     /locus_tag="IWK54_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9593404.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(24264..24629)
                     /locus_tag="IWK54_001276"
     CDS             complement(24264..24629)
                     /locus_tag="IWK54_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9593405.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(24792..25187)
                     /locus_tag="IWK54_001277"
     CDS             complement(24792..25187)
                     /locus_tag="IWK54_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9593406.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(25205..26167)
                     /locus_tag="IWK54_001278"
     CDS             complement(25205..26167)
                     /locus_tag="IWK54_001278"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9593407.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(26167..26379)
                     /locus_tag="IWK54_001279"
     CDS             complement(26167..26379)
                     /locus_tag="IWK54_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9593408.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(26400..27098)
                     /locus_tag="IWK54_001280"
     CDS             complement(26400..27098)
                     /locus_tag="IWK54_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9593409.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(27118..27660)
                     /locus_tag="IWK54_001281"
     CDS             complement(27118..27660)
                     /locus_tag="IWK54_001281"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9593410.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(27792..28796)
                     /locus_tag="IWK54_001282"
     CDS             complement(27792..28796)
                     /locus_tag="IWK54_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9593411.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(28793..29782)
                     /locus_tag="IWK54_001283"
     CDS             complement(28793..29782)
                     /locus_tag="IWK54_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9593412.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(29779..30585)
                     /locus_tag="IWK54_001284"
     CDS             complement(29779..30585)
                     /locus_tag="IWK54_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9593413.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            30751..31707
                     /locus_tag="IWK54_001285"
     CDS             30751..31707
                     /locus_tag="IWK54_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9593414.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(31784..32302)
                     /locus_tag="IWK54_001286"
     CDS             complement(31784..32302)
                     /locus_tag="IWK54_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9593415.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(32390..32539)
                     /gene="rpmG"
                     /locus_tag="IWK54_001287"
     CDS             complement(32390..32539)
                     /gene="rpmG"
                     /locus_tag="IWK54_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9593416.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            32767..33213
                     /locus_tag="IWK54_001288"
     CDS             32767..33213
                     /locus_tag="IWK54_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9593417.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(33408..35303)
                     /gene="asnB"
                     /locus_tag="IWK54_001289"
     CDS             complement(33408..35303)
                     /gene="asnB"
                     /locus_tag="IWK54_001289"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9593418.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT        10404 a         7491 c         6187 g        11494 t
ORIGIN      
        1 aaattcacaa agaaacgcag gtatacttac taaataatgc caaataaatg catgatagga
       61 aatacaaaca gcaaactgtc gaaacggtct aagattccac catggcctgg taagatattt
      121 cccgagtctt tcacatcata atgacgtttg attgcagatt cgaccaaatc accaaattgt
      181 ccgactgctg aaaaaataat cgtcatcaga agcatgacaa acataccatg attaaatagt
      241 tcttttgctg ggtatagcaa taaataaaga aatgcgacta caacagcact accaatacca
      301 ccgagcgcac cttcaatcgt tttgttgggc gaaatatcag gacttaactt atgctgacca
      361 taacgtcgac caaccatata tgctccgata tcagttgccc aaacaataaa tagaccaaat
      421 atcaaaacag ccaagctttc tgttcgtgca ttgacaaaat tctggaaacc tattcccaca
      481 tacaagctga caagcacagg aaaacctgct tcatcaatcg tatacgtatt tttagaaata
      541 actgatgttc ctaaaagaat cataactata agataaaata acataaatgt acttacattc
      601 tctggaagaa agaaaaacca tctttctttt ggcaaaacaa gtaacactgc accaacggcg
      661 gataaaactc cttcaaaact taagagtgtc aatcctttca tccgaaacag ctcgtaaact
      721 cctattactg ctaagattgc tgctgtaagc tcaaccgcta tgcctccgat ccatataatc
      781 ggaataaaca agactagcgc cacggctgct gtgaccacac gttgtttcat gactgatccc
      841 ctttctcatc ttctgttttt tttacaccgc cgaatcgacg gtctcgattt tgataagagg
      901 cgattgcttc ttcaagatgc gcaccatcaa aatcaggcca aagcgctttt gtaaaataca
      961 gctcactata tgcgatctgc cataacagaa aattactaat tcgctcttct ccacttgtac
     1021 ggatcatcag ctcaggatct tgtaactctt taggcaagaa acctgtcatt aaatgcttag
     1081 caatcaattc atcatcgatt tcttctgcgt gtatttctcc ttttgctact tcttcggcaa
     1141 tttcttggac agcagtcacg atctctgctc tgcttccata attcaaagca aaattcaata
     1201 ccattccagt attgtttttt gtttgctcaa ttgcacgtct tacagcatct tgcgtatgtt
     1261 ctggcaatac gttttcataa cccatgacat gaactttgac attttcttta atcaattctg
     1321 gtacgaacgt atcaaagaag tcgacaggta gttgcatgag aaaacttact tcatcttttg
     1381 gtcgcttcca gttttcagtc gaaaaggcat aaagcgtcaa cactttcact ccaagccttg
     1441 atgctttttt tgtgactttc tttactgttt ccatgccttc tttatggcca gcaactcgtg
     1501 gcaaacgacg gttttgcgcc caacgaccat tgccgtccat gatgatggca atgtgctgag
     1561 gaattggtcc atcggtatta aattgaaatt cgcttttttc ttgaatatat ttgttttttt
     1621 gcggaaaaaa acgtaacatt ctttttcctc ctatacttaa aagattccca ctatcttaag
     1681 tattatagca atatctaact gtttttccta tgaaatcatt gtttttttat ttaaaaactg
     1741 aataattttt tcttttttta atttttaaat aaaagaaata taaataaaaa cagaggaagc
     1801 ggaatgatcc gcccctctgt ttttatgatt ttattaaact tccaataatt ctttttcttt
     1861 atcagcagcg atcttatcta cttctttgat gctgttatct gtcaatgttt gtacgtcttt
     1921 ttctaatccg cgcaaatcat cttcggtaat atcaccattt ttttgttgtt ttttatattc
     1981 atcgatcgca tcacgacgga tgttacgaat cgcgattttt gcattttctg cttctttttt
     2041 aacatcttta gctaattctt ttctgcgttc ttctgtcagt tgcgggataa ctaagcgaat
     2101 gacatttcca tcattggttg gactgattcc gatatcactt gccataatag ctttttcaac
     2161 atcttgaaga atgctcttgt caaaaggtgt gatcattaaa acacgtgctt ctggaatttg
     2221 gattgatgcc atttggttca atggtgttgg cgcaccgtag tagttcacag taatacggtc
     2281 caaaaggcta gcgttcgcac ggcctgcgcg gatttgccca agttcacgtt gaagattttg
     2341 tgctgctttt ttcatttttt cttttgcttc tgtcataatt gcatctgcca tttttatttc
     2401 ccccttacag ttgttccgat attttcgcct aagattgcac ggcgaatatt tccgtgttca
     2461 tttaaattga agacaagcag tggaatatca ttatccatgc ttagtgaact tgcagttgaa
     2521 tccatgactt gtaatccttt ggcaataact tccagatgag tcagttcttc aaattttact
     2581 gcatttgcat ctactttcgg atcagcagaa taaacaccgt ctacattgtt tttagccatc
     2641 aggataacat ctgcgccgat ttctgccgcg cgtaatgctg ctgttgtgtc tgtcgagaag
     2701 tacgggttgc cggtaccgcc tgcaaagata acgatccgtc ctttttctaa atgacgttct
     2761 gctttacggc gaatatatgg ctctgcgatt tgacgcattt caatcgaagt ttgcacgcgt
     2821 gtcggtacac ccacgttttc caacgtatcc tgtaaagcta aggcattcat aactgtagct
     2881 aacataccca tgtaatcagc ttgagcacgt tccatgccca tttgtgctcc gatttgtccg
     2941 cgccagatat ttccgccgcc cacaacgatt gccatttcaa cgcccagctc gtgaacttct
     3001 ttgatttctt ggatgatttc tttgattact ggtggtttga ttccaaatcc tgcgtcccca
     3061 gctaaagctt ctccactcaa ttttaagact acacgctgat atttcggtgt gaccatgttc
     3121 attcctccaa tattttctat tgccatttta ccatacttag atgatttcga cactctaaga
     3181 ctggtttttc ttgttttgct taaaaaaagg gagcgcacaa accgtgcgtt cccgtctgtt
     3241 attcaacaga attatttttt cacttggctc ataacttctt ctacaaagtt atcttcgcgt
     3301 ttttcgatac cttcgccaac ttcgaaacgt acgaatgttt taacagttgc gccttttgat
     3361 gcaacgtatt tttcaacagt catatcagga tctttaacga atggttggtc caccaaagcg
     3421 atttctgctt tgaatttctt caagcgtcct tctaccattt tttcaacgat attcgctggt
     3481 ttgccttcgt tcaatgcttg ttctgtaaga acagtttttt catgttctaa ttcagcttct
     3541 ggaatttgtg attcgtttac ataacgagga ttgatagctg ctacgtgcat agctacgtct
     3601 cttgcaactg tttcgtcagt tgttccatct agaacagtca atacggcaat acgtccgccc
     3661 atatgcaagt atccaccaaa tgcagcattg tcgtcttttt ctacaacttc aaagcgacgg
     3721 aagctgattt tttctccgat aacttgagtt gcttcgatca agtcagattc aattgtacct
     3781 ttatctgttt tgattttcat tgcagcttcc atgtctgctg gtttgttttc agcaacaagt
     3841 tctgcaattt cttttactaa atcttggaac atttcgtttt tagaaacaaa gtctgtttct
     3901 gagtttactt caacgattgc tgcagtgtta cctttcacag caactgaagc caatccttca
     3961 gctgcaatgc ggtcattttt cttcgcagcc tttgccattc ctttttcacg taaaagatca
     4021 actgcttttt ccatgtcgcc ttctacttca actaatgctt ttttcgcatc catcattcct
     4081 acgccagtca tttcgcgtag ttcttttacc attttagctg taacgtctgc catgtttaac
     4141 tcctcctgtg tgtttttctt tttaaaaaaa gctgcttcaa aggagagtga ggcttgtcgc
     4201 ccagcctttg aaacagctcc atcattcaaa attattctgc tgaagaattg tcgccttcaa
     4261 caacatctac gatttcttcg attgatgttg ctgtgttttc ttctgtgaaa tcttcttcaa
     4321 ctacttgatc ttcaccttgg tttccttcga tgaaagcatc agccattttt gaagtaatca
     4381 atttaacggc acgaattgca tcgtcgtttg aagggattac tacgtcgatt tcatctggat
     4441 cgcagtttgt atcaaccata gctacgattg ggatatttaa tttgtgtgct tcttgaacag
     4501 caatacgttc tttacgtggg tcaacgatgt acattacatc tgggattctt ggcatatcag
     4561 cgataccgcc taagaatttt tcaagacgtt cacgttcttt gttcaaacca gcaacttctt
     4621 ttttaggtag aacttcaaaa gttccatctt cttccattgc attgatttgt ttcaaacggc
     4681 tgatacgttt ttggatagta tcccagtttg tcaatgttcc gcctaaccaa cgatggttaa
     4741 cgaagtattg tcctgcacga gttgcttctt ctttgattgc ttcttgtgct tgtttttttg
     4801 tacctacgaa taatgctacg ccgccttctt cagcaacgct cttcatgtaa tcataagctg
     4861 catctactaa tttaactgtt ttttgcaagt caatgatgta gattccgttt ctttctgtga
     4921 agatatattt cttcattttt ggattccagc gacgagtttg gtgaccaaag tgtacgccgg
     4981 cttctagtaa ttgtttcata gaaattactg ccatgtttgt ttcctccgat ttggttttat
     5041 ttttcctctt cttatcttct accttgccgg caaacttttt caagcaccta ccgacaatcc
     5101 aataagaatg tggatttgct gatgcataaa gcaccctcag ctattataca gacaaaagat
     5161 ccgtatcgca agagcaaata tacttttctg ctcaacattc aatagtttac ctgaaagata
     5221 aaaagaattc aagaaaaaca gtagatttct ctttgtttct ttattatcct ttttccataa
     5281 aataatttta ggtataaaag acgaatatta ttgggcaatc cttctttttt ttgttatgat
     5341 acatgtgaaa tctttcgtaa aggagaaaga aaatgcgcaa agctcaaaga catttgctga
     5401 ttaaacaagt cattgaagaa ttcccgatcc gtacgcaaga agaactatta aacaaacttg
     5461 ggcattatgg cgttaccgcc acgcaggcaa cgatttctcg agatatccgt gatctaaaaa
     5521 tcgtgaaggc acctgatgaa aatggcgttt cccgcttcgt attgtttcgc gaaaaaggaa
     5581 ctcctgagac taggactgag gaagaggatc ggctggttca aatgattgaa gacattgtat
     5641 tgaaagtcga acgagtccac tttttgacga tcgtcaatac cttgccagac aatgctcatt
     5701 tatttgcatc tgttttagat gagatcaaac ctccctctat tgtcagtaca attgctggat
     5761 tcgatacgat catggtcatc tcggaaaatg aggaggacgc aaaacgagta gaagaatttc
     5821 ttcaccatcc aactgaagta tcactttttt aatcagagca cgtattccta atatggaatg
     5881 cgtgtttttt tattttcaat attttcctta ttgaataaac ctttacactc tttacaaaaa
     5941 tacatcattt tttattcaaa tttttcaaca ccttgattga atcacaataa tcatcattta
     6001 tattggatgt taacaagagc agcttcttgc tctttgtgta tttcaaaaaa gaaatgaaag
     6061 gaaatcatat gtataaatta ttaacttctg gaccattgac gacttctgaa acagtgaaaa
     6121 atgctatgct gatcgatcat tgtacttggg atcaagaata caaagaaatt acacaatgga
     6181 taagaaaaaa attattggat cttgctcaag tatcggaagg aatatacaca acggtcttaa
     6241 tgcaaggtag cagctccttc ggggtagaat cggtattaag ttctgtgatc aggccagaag
     6301 atactgtact gatttgttca aacggcgcat atagacaacg aatgatcaac atgtgcgaat
     6361 gtctgaaact gaactatacc gtttatgagg tatcggaaca tcaaatcctc caagctcatg
     6421 tgattacaca gctgattgaa gagaatccgt ctatctccgc actagcaatg gtccatagcg
     6481 agactacttc cggtattttg aataatttgg aagagattgc tgctgtcaca caagaaagag
     6541 atcttttgtt tatagtggac gcgatgtcaa gctttggtgg gataccgatt tgtgtagccg
     6601 aactagggat tgattttttg atttccagct caaacaaatg tattcaaggt gttccaggct
     6661 tttcttttgt acttgctaat aaagcggaac tggaaaaacg aaaaaatgtt agccgaagtt
     6721 tgtcacttga tctatatgac cagtaccgcg tgatggaaaa cgagaatggc aaatggcgtt
     6781 tcacttcccc tacccatacg gttttggctt ttcaacaagc gctgaaggaa ctagaggcag
     6841 aaggaggaat cgaaactcga tattcacgtt atctcgccaa taatcatgtg ctacgacaaa
     6901 aaatgacaga gatggggttc cagcctttca ttcaagaata tcaggggcca ttcatcacct
     6961 cttttcttta ccctaaggac tggcgatttg attttaatca tttttatcag tatttaaagc
     7021 aaaatggatt tgtgatctat cccgggaaaa tcagcgatca tgaatgtttt cgtatcggaa
     7081 atattggcga gatctatccg gaagatattt tggaactgac agcaattatc aaagactata
     7141 aggagaacgt taatgattaa aactgtaatt tttgattggg caggaacaac tgtggacttt
     7201 ggctgtatgg ctcctgtcca cgcttttaga aatgcttttc tagaaaaagg aatccaactg
     7261 actgacaaag aaatccgcta accgatggga aaattaaaat gggatcatat ccaacagctt
     7321 ctttccctcc cttctgtcaa aaaacaatgg atagctattt atggacaact acctcaggaa
     7381 aaagaggtgg aggaattgta tcaacaattt gaacattatc tgtttaaaga tttggtcaag
     7441 cattctacct tgaaacccga cacattggaa acgatcgaac ggttgagaaa cgctgggatc
     7501 aatattggtt ccacgaatgg atttaatgct cctatgatga caatcgtagc agaaaccgca
     7561 aaaaaagaaa ggtattcacc agacttcatg gcaacttttg aagacgtcga agggtatggt
     7621 cgaccgtttc cttatatgat ccataaaaac atgcagtact tcaaaaacaa gtcggttgat
     7681 gaagtaatca aagtcggaga tacggtctca gatatccagg aagggaaaaa tgccggagtg
     7741 ttgacagtag gtgtaattga aggtagttcg ctaatgggga tttcttatga tgagtaccaa
     7801 aaattagaga tggggaaaaa gatcgtttaa aacagtctgt tgctcttaga ttccaacaag
     7861 ccggcgctga tatttgtgta aacaatttaa aagagttaac agaatacatt ttgtacgctg
     7921 acatgaatcc agaatgactt ttatacaaaa taagaggttg tgaaaaagct gtcctgtatc
     7981 aagtaataaa aacagccaag caaaaatagc tcctcatatt tttcgtttgg ttaggcttat
     8041 taccgcagat gcaagctttt gaacaccgtt tattcgaaaa aaaaaaggga gctgtgacac
     8101 gacttttgtc acagtcccct tatttgcaaa caaacagtgt ttaagagaca acctctccac
     8161 taaacggtgt tcattcagct gactctcggt atttgctcaa aaaacgataa aatttgaaaa
     8221 gctattttct cttttgtttc tctaatactc ggatcagaac gctgatttca cattctcttt
     8281 ttcttacatc ttatctggtg catgaagtcc tagcaaacga aggtcttctt tcaataatac
     8341 agtcactgca taaactagag ctaagcgtgc ttctttttgt tcatcatcag ctaaaatctt
     8401 cgtatgagca tagtatttgt tgaatgcttg tgctaatttg attgcatgtt tcgcaatcac
     8461 tgacggttcg tatttttccc cagcagacaa gaccgtttca gggtattttt gaactaattt
     8521 aaccacttcc cagctggtat cgtcattcaa tgcatagtct gctgtttctg aagggacaaa
     8581 tcctgctttt tccaacaggc tgactgcacg agcatgtgta tattgcacat aaggtccagt
     8641 ttctccttca aatcgaacaa cttcttccag gttaaagtca aatgtattca gtcgatcgtt
     8701 tttcaaatcg tggaaaatga ctgccccaac cccaacttgt ttagctacag catctttgtt
     8761 ttccaaatca gggtttttct cgctgatttg ttctttagca gattggattg cttcatttaa
     8821 tacttcttct aataagacga tctttccttt acgagttgat aattttttac caccttgcgt
     8881 gatcaaacca aatggaatat ggtgcatgtc gtcagaccag tcaaagccca tttccttcaa
     8941 gacagctttc aactgtttaa aatgatagct ttgttcattc cctactacat atagagattg
     9001 tttgaagtcg taagtacgtt tacggtacaa tgcagcagct aagtcacgtg taatgtatag
     9061 tgtcgcaccg tctgacttct taatcaacgc tggattcagg tcataagcag ataggtcaac
     9121 gatctctgca cctttatcct cgttcagcaa atgtttttct tctaaaagtt tgacgatttc
     9181 atccatcttg tcattgtaga aagcttcacc gttcaacgaa tcgaaacgta cttccagcag
     9241 atcatagatt ttattgaatt ctttcatcga ttcatcacgg aaccattgcc ataattgaat
     9301 agcctcttta tcgccttctt ccagacgttt gaaccaagca cgagcttctt catttaattc
     9361 tggttctgtt tcagctactt catggaattg gacgtataaa cgaagcaatt cattgattgg
     9421 ttctgcttta acagcttctt ctgtacccca ttttttgtaa gcgacgatca gttttccaaa
     9481 ctgtgttccc cagtcaccta aatggttgat gcggattggc tgataaccga ttttttccat
     9541 aatgaaaccg atcgaattac cgataactgt cgaacgcaaa tgtcccattg agattggttt
     9601 ggcaatatta ggagaagaca tgtcgattgg tactgttcct tgattcccga tgttgctatc
     9661 cccataatgt tctttttctt tcactactgt ttgcaaaact tttttgctga taagttcttt
     9721 attcatgaaa aagttcaagt aaggtccgac aacttcgatt ttttcaaagt tggcagaatc
     9781 gattttttct gctaaatctg cagcgatttg ctgtggcgct tttcggtaaa cttttgctaa
     9841 tgaaaaggct ggaaatgcaa catctccgtg ttcagctgat tttggatttt ctagtaattg
     9901 ctcgacttgt tccagtgtta aatcatcttt gacagcgtca tagacagctt ttgcgacaag
     9961 atctttatta ttcatcttta ttcctcctaa agtgttccct tgattgttaa acaaaaaaaa
    10021 tctctagcga cggcatactt agtatgccat cactagagac gagatttccc gcggtaccac
    10081 tccaattgtc ctaaaaaagg accagctcca tctgatatcg gtagacgacc gtctgtttaa
    10141 aacagaattc tccaagtgcg cttcgttgta tcttcttcat tcggcttcca ccacttccga
    10201 attcgctagc caagaatgat acaagtactc tcttgttcaa caaatcttag tcttattaaa
    10261 ctttcttgaa ttatatcaga aaaaaaaacg aaaggctagt cgtttcataa aaaaacaatc
    10321 cagatggcct aaaggagaag aaataccacc tggatagcta taaatcattt cgtaaatacg
    10381 aaagacttaa caaattaatc agctacgata atcgttcctt cttcagaagc taaaagattc
    10441 tcgatatttt ctaaagaagt gatgattgcc ttagcgtttg gttttgcttc aacaaatgca
    10501 atagctgctt ctactttcgg aagcatgctt cctggagcga attgcttttc atcaatgtat
    10561 tgtttcattt ctgaaacagt aacagtttct aatttctttt gatctggttt ttggtaattg
    10621 acatacacat tgtctactcc tgtcaatacg atcagcaaat ccgcatcgat gatttcggct
    10681 aatttttctg aagcaaaatc tttatcgatt acggcttctc gtccttctaa tccagtagct
    10741 gtttctacaa caggaatccc accgccacca acagagactg tgataactcc ttgatcgacc
    10801 agcgtttcga tgacacgtgc ttctttaatc gagatgggtt taggggaagc gactactttt
    10861 ctccatccac gtccagcatc ttctttgaat gtcacagtgg agtcagcatt catttgttct
    10921 ttggcttctt cttccgtgta gaatggaccg actggtttac ttggattttt gaatgaagga
    10981 tcattttcat ccacaataac ttgagtgact aaagaaacca catctttatc gatccctttt
    11041 tccttcagta cttcgcccat ggcgttttgt aaccagtaac cgatagatcc ttctgtcatt
    11101 gcaacacatg tatctaaagg cattgccggt gttttctcag aatcagcagc ttgctgctga
    11161 atcaataggt taccgacttg cggaccattt ccgtgggaga tgatcaattc atctccttgt
    11221 tcgataaatt tcactaaata ctttgctgtt tccattaacg cttcttgttg ggcttttgca
    11281 cttgcatccg tagacaagat ggcattgccc cctaaagcga caactacttt tcgtttggcc
    11341 atttcattcg tcctttcttc agttctaccg aattttctag ataacaaaac gagccagcgt
    11401 tttgtgattt tccctcaatg ctgactcgtt ttttcgtgag atgaatgtgg ttatgcttaa
    11461 acgcgaggga taaacaagtt acctaatgtt gctgccatga tcgctttgat tgagtgcata
    11521 cgattttctg cttggtcaaa ttgacgagca tatttgctgc ggaagacttc atctgttact
    11581 tccatttcag tgatgccaaa gcgttctttc atttgttcac cgtaaacagt gtttgtatca
    11641 tggaaagctg gcaagcagtg caagaagatc aaacgatctg tgttgtgtgt tttttctacc
    11701 atttccatat tgatttggta aggtttcaac aatttgatac gttcttcgaa tttatcttct
    11761 tcacccattg atacccatac atcagaataa aggacgtctg ctccgtctac acctttatca
    11821 acatcgtcgg taatcatcaa ttgtgcaccg gatttttcag caaagccttc tgccattttg
    11881 acgatttctt cttcaggttg taattctttt ggtgcaacga tacgcatatt cgctcctaaa
    11941 attgcacctg tgactaataa agagttagcc atattgtttc gaccatctcc gcagtaagca
    12001 acagtgatac cttctactgt tccaaagttt tcttggatag ttaaaaagtc agcgatcatt
    12061 tgtgttggat gccattcatc agtcaagcca ttccaaactg gaacaccaga gaattctgct
    12121 agttcctcta ccattttttg actgaatcca cggaattcga ttccgtcaaa catacgtcct
    12181 agtactttag ctgtatcttc tgtggattct tttttaccta attggatatc atttgcacct
    12241 agatattcag gatgtgcacc tagatcgatc gctgcagtag taaatgcaga acgagtacgt
    12301 gtagatgttt tttcaaataa aagcgcaata ttttttcctt ctagatagtg atgtgggata
    12361 ccacgttttt tcaagtcttt caagtgttct gaaaaatcga tcaaatattg taattcttct
    12421 tttgtaaagt ctttttctgc taataagctt cttccttgga atactgattc tttcatatta
    12481 aacattctcc tttagattgt ttttatttaa gattaaagag gaaatcccga cggcagctga
    12541 cagtaagact gccgtcagga aacaaattac ttcaagtctt cacggacaag tggttggctc
    12601 atgcagcgtg gaccaccacg acctcgtgac aattcacttg aattgatttc taacactttg
    12661 atgccatagc tacgaagcaa ttcgttcgat acataatttc ggttataagt gactacaaca
    12721 cctggagcga ttgccaatgt gtttgatcca tcattccatt gttctcgagg tgcaacgatc
    12781 tcatctccat taccagttgg gatcagtaca agatcatcta atccaagagc agctttcatc
    12841 gtttcgtgta agttatctga atgagtaatc ttgatatcat caccatctgg ttcgattgta
    12901 aagacatcca tcttaccgtc tttactttga attgctggat ggatagtgaa tttatcgtga
    12961 tctaccattg taaatacggt atccaaatgc atcattgcac ggttgttcgg aattttgatt
    13021 gccaacactt tttcaaatcc tgaatttttt gcgaataggt ggcgtgctaa tttttctaaa
    13081 gctttcgcat tagttcgttg agaaatccca acagctacta ctttatcgct taagatcaat
    13141 tcgtctccgc cttcgatatg atctggattt tcacgatcca accatacttc gattccttta
    13201 tttgcaaatc ttggatgatg tttcaaaata tattctgtaa acatcgattc acgacgacgt
    13261 gcttcaaaag tcatcttatt gactgtcatc ccatttccca ttgatgcgga agggtcgcgt
    13321 gtaaagtaca agtttggcat tgggtccatg taaaatggat agtcatcgtc tgctgacaga
    13381 tcatatagac tgctagaacg tacttcgata tcttttgtac gaaccccagc catgatttta
    13441 tctaccattt cttgcgtttc cattgataga aggaactcat gaagaccctc tcttacagca
    13501 ttcgatgcaa tatgagattc atccaacatt ttattcaaga attgttcttt gacattgcct
    13561 gcgtcaatcg cttctgccgc taatttttcc aagtaaagag tctcgacgcc ttcattttgc
    13621 aatgtcttcg caaagttgtc atgttcttct tgagcaatcg gaagatatgg aatatcatcg
    13681 aacaacagac gatccataat atctggtgtc aaattttcca cttcttgtcc aggccgtttc
    13741 agtaaaactg tctttaattt ccctatttca gagaaaacgt gaataggttt atccatgttg
    13801 tgttgcctcc ttctttttga ttacatgtat aggatatcct ccttttgtaa tcactttcat
    13861 aattttgtct cattcaaaat gagacattcc tcacattaat attcgataat taaataatta
    13921 aacgtttttc tatttttaag cgtaatttat tcatttttta ttctgtatac actttcaaaa
    13981 aaatatttat tcattgaaaa aatttttttg aataaattaa aaaaataaat tatattatcg
    14041 ttgttagcgt ttactatatg tattattgtt gttttttata cacaaatcat tcgcttcaaa
    14101 acaaattatt caataccatt gtatattttt tacataataa caaattatat ttgtaaagta
    14161 ttaaaacttg tttctaatgt tggtatgtta aaactttaca aattctttaa aaaaagtgac
    14221 aataaatata gtgaacaata aggagaggac tttttaatgc gtgttaataa aatcgatttt
    14281 cacttttcaa aactcagata tcaacctgat tttgaacatt tctcggatga agagttccaa
    14341 cagatcaagg aacatactgt tcttcgttct tataaaaaag gacagatcct tttcgacgat
    14401 ggtgatccca gacaacggtt gtatatcttg accaaaggac tcgttcgttt agaacgttat
    14461 gatgaaagtg caacttatta ctactatgac tatgtgaata caaataacct ttttcctatg
    14521 tcaggaatgt ttggagaaga gacctacact tatacagctc aagcgatgac tgatattgaa
    14581 acattctacc ttcctgtttc ctactatgaa aaattagcac gaaaaaatcc cgaacagttg
    14641 atctatatga tacaaaaaat ttctaaagta gttgaaatac atgaattgcg tatccaaatc
    14701 ggattgacct ctagcgcctt tgaccgggta aaacaaaatt tattcatttt gaaagaagaa
    14761 ttgggcgtac caacaaaaga tggtcatatc accattcctt atccgatcac cttaaaagaa
    14821 ttagcagtga atagcggcac gacacgtgaa actgctggac aagtaatcaa acaactacgt
    14881 gattccgggc tgttagatta tgacaaaaag cgttttactt tctgtaagac agctactact
    14941 ccagctatga gagaataacg cgaacaaaaa taagcagtga ccctgataaa tatcagggtc
    15001 actgcttatt ttcctcccta aaacttctga aagatccggc agttttagat cactggacaa
    15061 gactcaaaga ttttctatgt gtgtttcagt tatgtaatat tcactgtttg caaaaaatac
    15121 tgttagatta actagatgaa gcgtcttgtc ctcatccgat ctagattatt cgatattcct
    15181 cgacaataaa cgattactca gtgagggcaa tcagaggaac ggataatgat aaataagctg
    15241 taaaaaatag caaaataaca ataatgatta aaagtatgta aaaaataggg ctttgacaaa
    15301 aacgtgtcac agccctattt cgattaaacg gtgtgtacct gtcaactctt cggtcacaag
    15361 tcataatgtt cataaacatg aggagctgtt ttttgaaatt gttccttgtg aatcagagtt
    15421 aaacgacagg cttacgaact cttgattaaa cggcgttcaa aagcttgcat acgcggtaat
    15481 aagaacagcc aggtcaaaaa catgagaagc cgtttttcct gggctgttct tattacttga
    15541 tgcagggcag ctttttcaca acctctttat tcagataaaa ccaagcttgg atcagcattc
    15601 aactgatagc tagcaaaatt tcttttttgc atttccacat gtgctgctgc tccaatcatt
    15661 gcagcattat ctccacaaag tcgaagaggg ggaatcacta gttctacctc agggagttta
    15721 gctgccaaag catcctgcaa tccttcacgc aacccttgat tggccgctac acctccagct
    15781 ataatcagtt gcttgactgg ataattttga caagctcgaa gtgttttagt aatcaataca
    15841 tcgatcacac ttgcttgaaa gctagctgct aaatcatttt tgtctagatc ttctccacgc
    15901 tgttgtgcat tatgcaccaa attgataaat gcacttttta atccgctaaa actgaaatca
    15961 taattatctt catggatcat cgctcgcgga aaatggtaat tatcttttcc ctgatgagct
    16021 agctgatcga tttcttttcc gcttgggtaa gaaagaccta atactcttcc aactttgtca
    16081 taagcttctc ctgctgcgtc atctcttgtc tccccaatga tttcatatga tccatcttct
    16141 tgcatataga ctaattctgt atgtcctccg ctaacaagaa gtgccatcaa tggaaactgg
    16201 aatggtttaa ccagacgggc tgcatagata tgccctgcca tatggtttac aggaataagc
    16261 ggcaattggt gtgcccaagc aaaagcctta gctgcagaaa taccaatcag caacgagccg
    16321 acaagtcctg gtccataagt gacagcgact gccgataaat cttctgccga cacaccagct
    16381 tctaccagtg catcttccaa gcataaggtg atttgttcta cgtgatggcg gctagctact
    16441 tcaggtacta cgccaccaaa acgtttatga ctgtttattt gagaagcaac gatgttagat
    16501 aagatctctg ttccatttct aacgaccgct acactcgttt catcacaact tgattcaatc
    16561 gccagaatca attcttcatt catttcatcg tctccgtttt taactttctg ctcataatga
    16621 tcgcatcttc cgtcggattg cgataatatg atttccgttt acctaatata cgaaactttt
    16681 ctgattcata aagttttctt gctgcttcat tagatagacg tacttctaaa aaaacatgca
    16741 gcgaatcttt tatttgttcc gtaatcagta attgtaacaa atgccgtgca tgcccttttc
    16801 cttgttctga aactgcaaca gcaatatttg tgatctcgac ttcatctatt actttcgagt
    16861 atccaataaa gccacagatc tggttatctt cagctaaaag cagataatca gagtgaggct
    16921 gttggatgtc tgccaaaaac tgcattttcg tccaaggtga accatgtgga taagcatttt
    16981 cgctaatcat ccatacgcat tctgccaagt acgcttcatc aaaatctttt tttctgcaaa
    17041 tcaactaatc tcactcattt tctaaaagtt ttatcatatc caacgcatct tcttttgttt
    17101 cagtgtaata tctttttttg atcgttgttg cttgaaaccc caatttccga taaagtcttt
    17161 gtgcatcttg attactcata cgcacttcta atgacatcgt ctcacagcga ttcattatag
    17221 caaagttctc gatttcgtca ataagcagcg aaccgattcg tttacgctga tatgccggtc
    17281 gaacggcaat attcgtgata tggcaatcta aaccgaacgc tcttgaaccg ataaaaccaa
    17341 tcattcttcc attatcaaag atacctagat atagatgagg aagcggtgac ttcaattctg
    17401 atataaaggc actttttgtc catggcaatt cgccattata gacttctcgt tcgacatcca
    17461 gcagctcttt gatatcttct gtagtcaatt ctcttagtac gtaacgacga tcgatttgtt
    17521 cgactgtttt ttctgtataa atcattttat cttgaaaaaa gcttctggca tacgctctaa
    17581 attttttcaa cataactttc gtctccaggt gtgtgcgtct ccaaccattt ttcttctgct
    17641 tctactcgtt ttaaatagcg aggaagaaaa gcttgtttct ctgcaactgg ttcaactaaa
    17701 gcacctaatt gagccacagt tacaccactt ggaatatccc atgctggaac tgtattaatc
    17761 atcgcactag gcaatatttc ctttatttca gagaagaatt tgtgacaatc actcccgacg
    17821 aaataaatag cttcattcag cttttgtagt tgtgtgaaca aatcagaagc agaaatatgt
    17881 acatcttcaa gtacagtttc taatctattt tctttccagc ggtatgcccc agcgtataca
    17941 ttttttcttc gagcatcaaa catgggcaca ataatattat ccacaccgat gcaattagcc
    18001 gcgatggttt tcagactaga tactcctact agctctttat ctaatgtata agcaatcgtt
    18061 tttgctgtcg ttacacctat acgaagccct gtataagaac cgggaccatc agaaaccgcg
    18121 attcggtcaa tgtcttttgg acttatttga agatctgcaa acaattggtc gattgcaggc
    18181 atcagtgtta cactgtggtt gcgtttgata tttgtttgta tttgtccaag aatttgttcg
    18241 tcttctacca caccgatcgc caaagtttgg tttgcagtat ctattcctaa tgtaatcata
    18301 tatttgatcc tttctgctat gacattctcg ccttccattg tagcatactt ttttattctg
    18361 aggatgaaaa taccaaaggc aaaactagta caataataca tgattatctg ctggcgggca
    18421 aattttgccc cttcctgaaa cacaaaaaag ctgcggtatc actccatttt cccacagctt
    18481 ttccatcttt tatttcgttg attctcgaat aatcaactct gtttccacaa taatctttct
    18541 tgaaaccggt gctttggaat tacttaattg aagaatcgtc tccactgcta gctctcccat
    18601 ccattcggta taaactttta cagtagaaag cgagggcgtt acatatttcg ctacactgac
    18661 atcattgaag cccatcacag aaatatcttc tggtaccact aaaccttttt cttgaatcgc
    18721 tttcagtgcg ccaatcgcta atgcatcatt agctgcaaaa agcgcatcag gaacatgcgg
    18781 attcgttttt aaatattgct tcactgcttg atatcctgac tcgacagaaa aatctgcttc
    18841 gataattgga ccttcgtcgt agcctttttc tgccaaaact tgttgaaatg ccataattct
    18901 gggatctttt agccgctgat ggtttttctt cgtatattct gttccagcaa gcatagcgat
    18961 atgttgcgct ttatgggaga caaagtgttc aatgactttt tgaacacttg acttaaagtc
    19021 gacaactaga gagtcgattc cttgagaagt gccgtcagaa tctaccaaaa gcaaggcatt
    19081 ttgggatcct ttcatttcta ataactgagc ttcgtcgaat tttcctagtg cgatcgttcc
    19141 atctgccgga agatcactaa tggaatccca cgtttctttc aataaagtaa cgtttgcttc
    19201 ttctgccttt ttttcgattc ctaggcgaat agataaataa taaagatctt caagctcttc
    19261 cgtatcgtcg taccactgga tcaggcgaag cgtttgctta gtatttgctg catttttacg
    19321 atgtttcgta taatttaagc gttctgctgc ttcaaaaatt ttttgctttg tggctaagcc
    19381 aacggaaaga tcaggatcat agttgagaac tcttgaaaca gtagcaggag aaactcctgc
    19441 caactgcgca atgtctttaa ttgttgccat ttgtctctcc tcctttttta cgtttataaa
    19501 atcgcagtaa atcgttcaaa cgctgcctgt cctttttcat ctcgcttgaa tactccagcg
    19561 tcttccagta cacgtgcaaa aacctgacct acagattttt gtacgacatc ggtgaccgta
    19621 tctttcgtca aatcaggatg ttcttttttt aattgttctg cccacggacg atgatattca
    19681 gccatctggt tttcttctcc taataagaaa tgctctactt ctactagttc tggtttcaaa
    19741 cgaggtggta aaacagcaag ccccattact tcgatcaacc caatgttctc ctttttgata
    19801 tgttggacat ctttatgtgg atgaaaaatc ccatcaggat gttcttctga aacattgttg
    19861 tctcgaagga caaggtccaa ctcaaagtaa ttgtcccttc ttcgtgcaat tggcgtaatc
    19921 gtatggtgtg gtgtaccatc tgctgaaaaa gctctgatag atactgtttc atccgaatat
    19981 gctttccatt tttccaatac tgagttagct gcttcaataa gcaagttttt ctctgttgat
    20041 tgcaaacgga tcacagacat cggccatttc acgactcctg cgatgacttg cggataatct
    20101 tctagtttga taagtttttc tatcggtgct tttgccatag gaaattcatg acgtcctgcc
    20161 tgataatgat catgagaaag aatcgatcct ccaacaattg gtaagtcagc attcgaacca
    20221 acaaagtaat gaggcaagac ttcagtaatc cgaagcagtc tagcaaatgt ttcctttgtg
    20281 attttcatcg ggcggtgttg ttctgataaa atgatcgaat gttcattgta ataagcataa
    20341 ggggaatatt ggaatcccca gctttcaccg tctagattca ttcgaataat ccggtgattg
    20401 gtgcgggctg gatagttcaa gcgacctcta tatccttcgt tttccatgca aagcatgcag
    20461 gtaggatagt ctacttttac cgcctcgcgt tctgctgcga tttgttttgg atctttttct
    20521 ggcttagata aattgattgt gatttccagt tcaccatact ccgactctgc cggaaatttg
    20581 atgttttttg cgatggctct tgttttgatg tagtcatttt tcttacttaa attaaagaaa
    20641 tactcagttg cttcctctgg gcttttcgaa tagtgctgtg caaagaatgc attgacgact
    20701 gaaggtggcg gtgtgagaaa atccattaat tgcgcttcaa aaatctcttg ttctgctggc
    20761 aattctgaaa gaacaccatt ttctcttgcc ttagcgacaa gctgatccaa taagtctaca
    20821 gattcttcct taactggtct tacttcaatg ctttctagag catcttctcc taccatccct
    20881 aaaagacgat tctgtaaata taagcggtcc atttccatcc atccgccagc ttcgatcgca
    20941 agtgtggcaa aatcacaaat cgtttgactg attgacatat ctcattcccc tattcttcat
    21001 aaccatttgg atggctgaca tgccaatccc atgctgtttt gataatcgct ttgatatctg
    21061 tgtattcagg tttccagccc agaatctctc tcgctttttc acttgaagcg actaatcggc
    21121 taggatctcc agctcttctt ggtgctattt ctgctggaat ttcttttcct gtcacttctc
    21181 tagctgcttc tagcatttcc ttgactgagt atcctttgct gctgcctaaa ttgaagaagt
    21241 tgctttcatt tccttctttc aagtattcca aagctaaaat atgtgcagca atcaaatctt
    21301 ctacttgaac ataatctctg atacatgttc catctggtgt atcataatcg tctccataaa
    21361 cagccaaggc ttttctttga ccaagagcta cttgtaaaat gattgggaca agatgcgttt
    21421 ctggtgtatg atcttctccg atagatgcat ctgcttttgc tccggctaca ttgaagtagc
    21481 gcaaagcgac atagcgcatg ccatatgctt ggtcacacca cttcatcatt ttttccatca
    21541 tcaatttact ttcaccatac ggatttttag gattagctgg tgtatcttct gtgattggag
    21601 actctttcgg ctctccataa gttgctgcag tagaagagaa tacgatttta ttcacgttga
    21661 actcgtgcat gacttcaagt aagatttgca taccgtatac gttgttgttg aaatacatca
    21721 atggtttttc gacagattct cctactaaag agcttgcagc aaaatggatt actccttcaa
    21781 tcggttcctt ttcaaatacg gaacgtagaa attctttatc ccggatgtct ccttcataaa
    21841 aatgagcatc tggatgtact gcttgtttgt gacctgtgag caaattatct accacagcta
    21901 cttgataccc cttttgaact agctggtcta cagcgtgcga accgatataa ccggctccgc
    21961 ctagtactaa aattgtcata aaaaacactc ctttgattag taaaattgtg tttgttgatt
    22021 ctatataatt taaaaaacga agtttacttt tatggtgaaa acaaacaaag taaaatttcg
    22081 taaattcata tggtaaattt ataataaaac atttagtaaa cattttcaag ggttttttgc
    22141 aaaggtttac aaacaaacgt aaaactagcc tttttttctc ttttgttcca taatagatat
    22201 aacaaataat aaatgaggtg gattcaatga aaaaatttgt atctggaata ttagtaggaa
    22261 gtctggcaac agccgctgcc gttgctggtt tggttgcttc tgtgaaaaaa acagtgatcg
    22321 atccaattga cgaaaaagaa gcaatgatcg aagaaaaccg taaaaaagca atgcgtaaac
    22381 gcgtttctcg ttaataatta tttaaaaata cgcatgaaga ctttctaaaa aacagataag
    22441 tcatcatgcg tatttttatt tgtcagtcaa tataagttgt gctattttat ctgcctgtct
    22501 cagattccat ggatctgtat aaggtctagc tactgctgca gttagccaat ccacaccttc
    22561 aacaggtatt ccatagcaat aaaagtcaga ctggatttcc cctgaagcat caatcacgcg
    22621 attcgtacct cgctcaacat ctatagcttc tgaatgataa cttcctgtat tatctgaaaa
    22681 tgaaaaaggg cgaacaagtt tgttttcctt caaatttttt atgacaggat tacttgaacg
    22741 agaaaagctt gtttgtggca atcgagcttc aatcaaatag cgactgctga tttctttatc
    22801 ttgtagagaa ataacatagc ggttgtgctt ttgctgaata tcaaatggcg gttcgatcaa
    22861 atgaaaaata cccgcatcta tcagtgctgc taattctttc gtccgaatga ttggcggacc
    22921 gatcgttaaa aagctattta gatgagtaaa ccatccccat agtatttctt tgtattcctt
    22981 agcagaaaaa acgtgccatt caatagcctg ccggattgga tcacgccagt cttttaatgc
    23041 atcaaatgtt gaagcaaatg ctcctgtgca attgcctttt tccgcttctt ttatctgccc
    23101 ttcaataaat attcggctag cttctgaaaa gggctcattc tgataggcaa gaggagtttc
    23161 taaatacgac caatcccatc tataagggat tagtgctgga tgctttccga gacactcctc
    23221 tgaagagagg gagagaaaat catgctcaaa atttttcttt ggaatcggca agtgatattc
    23281 ttcgatgatt ttcttgtaat aaaacaactc aacgtctttt ttcagatagt caaaaaatgt
    23341 ttcgccagtc aaaaatcccg actgatgcca ttgctccagg ttttcttttg ttagtaaccg
    23401 tggccatgcc attgctccgc cttgcttttg atttcttcct cgagggtaat aagggagccc
    23461 ccttcttgat ccactgtata caatcggttc cttgcctgat ggatgataga ccagttttcc
    23521 cttttcgttc tcgaagcgcc ctcctcttga tactgtaaac aatccgacat aatcaaaaaa
    23581 acttaagcct agacctctta aaaagacatt ttctttaggc ggtatcttcg aaagatctac
    23641 atctgcagga ttagaaggaa gctgatacag taaaacattt ttctttgcgt agtctaccca
    23701 cactctttct tcttctgtct catcattttc ccagtgaccg cttgctaaaa caagctgatc
    23761 gatagaatag ctttgctgat ctgtctgtaa aagataacca tcttttcttt tgcttacttt
    23821 ttttactaaa gaataaacaa actgaataga agctggaaat tcttcttgca actttgagaa
    23881 aaaccagcgt tgatagagtc cgtataaaca tcgagtagat tgctgatctt tttccagacg
    23941 attgatttct tctataatta gttgcttatt tttggttacc gtctgttcga tataatctgt
    24001 agcaggtcct ttactccatt gaaacaagtt gggtccctgt gttaccaatc ctccactgga
    24061 caatgtttca tctgtaaaaa gcgtcacttg ttgagaaaca gaattcatta ataactccgg
    24121 tggctgattc aaccgccaaa ctcgacctcc aggaccattg ggatcgaaaa gaaaaagttc
    24181 aagagaatgt ctgtcctgat tattatggag aatcctttct gctacactta atcctctagg
    24241 tccggcacca atgatcccta ttttcatttg catgcctcca tttcgataaa ttttttcaat
    24301 aaaacagcat ggttctgatg ttcatttttt gcaccataaa caaaagtaat gactggatgt
    24361 tctttcataa gttgactgag tttaaaaata gcttgcttct tttcttcttc gaaaatcaat
    24421 tcttgctggt atttctttgc aaatgaagca aatcgttctg gtacatggtc aaaagttttg
    24481 cgtgcttcat tactaggtgc aatttctttc aaccacagat ctaactgcgc tttttctttt
    24541 gaaattcctc gtggccagaa tctatccacg agtattctat agccatcctc tttttcagca
    24601 tcaaggtaaa cacgttttaa tttgatcata gttgtttcct tctttctacc ttttcgctta
    24661 gcttatatat gtagataata gcacaaaata aaaaaaatga acggcacaac tatccattgc
    24721 gccgttcatg tgatcttggt caaaatctaa tgattgattc ggtttttaaa gctgtcatac
    24781 aatcatctgt tttatacttt tttacttttg atttttccag accatccgtc atatccaccc
    24841 ttaagtaaat aaatctcttg ataacctgct ttacgtaatt tatttgctgt acggatgctc
    24901 aaacttttct tttgatcgta aatatagaca ggctggtctt ttcgaataga tcctaatgaa
    24961 tctttcaaaa cagtgtaagg gatgtttctt gcacctaaaa tatgcccagc atcaaacaca
    25021 tctttttcgc gaacatcgat tacttgtgct tttctcattc cttctcgaaa ttcttcttct
    25081 gtcaaagtcg ttgctgaacg tttggccatt actcgtagat agacttccca aaggatgatt
    25141 gcaagcacaa tcaatagcaa aatgatatta attacccata aaaccatgta atgcctccgt
    25201 ttttttaagc aaatttcaag gctaatgagg cagcaccaat gacaccagct tcattaccaa
    25261 gctctgctaa tttgattttt gttgattctc ttacttgcgg gaaagtaaat tctttaaaat
    25321 atttctctac acggctacgt aaaaattctc ctgctgccga tactcctcca cctaacacga
    25381 tacttgacgg atttaatgta ttccccaagt ttccgcaagc taaacctaag tagaagcata
    25441 cgcgatcaac aaccatcaat gcaaacggat cgtcttcttg agccatttca aagatatctt
    25501 tacttgttac ttcttctccg ttgtctagca ttgctttcag ttttgaatcc cctgcgtatt
    25561 cttctgccaa gaaacgacct aggcgtacca cacctgtagc gctagataca gtttctaaac
    25621 atccgcgttt accgcaagta cattcgaacc cttcaggatc cacagtgata tgtccgattt
    25681 ctcctccagc tccgctcacg ccgtgaatca aatttccgcc agcaacgata cctccgccaa
    25741 cacctgttcc tagagtaacg aatacaacgt ctggatcgtt ttctccagca cctttccatc
    25801 gttcacctaa agcagcaaca ttcgcgtcat tatcgatagc gaatttgatt cctgttcctg
    25861 cctcgatcag ttttttcaca aattgaactt ctgtccagtt taagttatac gcaccaataa
    25921 ccgttccagc ttcgctgtcc acgcttcctg gggtacccat gccgattccg ataaagtctt
    25981 cggctttcat attatataaa ttcaaacgat gattgattga ttcaatgatt tcaggaacga
    26041 tatgcttacc atcatccaaa atattggtat cgatgctcca tttttgctga atctctcctt
    26101 ctggtgttaa aattgcaaat tttgctgttg ttccacctaa atcaatccca ataatcttct
    26161 tgtccattac aaatttcctt tctttcggct ttcttcaatt cggtgctctc tttttaatat
    26221 gcgccacgct gtcatgtatg tatcatgatc aatcaaacgg ctatcataca gattacgcaa
    26281 ttcgatagtc attaattcaa tgtcatagat gcgtgcaccc acatagataa agatgccaaa
    26341 ttttttgaac agctgctgta catcatataa gctctccatc tttaagcact tccttcatat
    26401 tatacaagta aaccatattt ttttaaaccc agtaaaatac atattacaaa aagaaataca
    26461 aaaactagtg tagaaagaat cctttggtgt atcgaatagc ttgtctgttg tttcggtaga
    26521 cccaaaacat tccctaacac taatccgcca attagcccac cgacatgtcc ccaaacatct
    26581 acagaggtat ccagaagacc aaaaacaaat gtcaacacaa taaataaaag aaaatgtctg
    26641 actagctgct ggatcgctgg gttatgttta aaatgaaacc ctaagataaa caacgcaccg
    26701 aacaaaccga atatcgaagt acttgcacca ccagacagaa cattcgcttc gttaaaagca
    26761 aagcttgctg tatttcccat tatcccgctg aacatataaa tcaagaaaaa acgccaatgg
    26821 ccatagattg cctcgatttg ctgccccata aaatacagta tcagtgagtt cattacgaaa
    26881 tgcatcagtc caaaatggat gaagattggt gtcactaaac gccaccattc attgaagtga
    26941 acaagataag gaccatacat tccggcataa taagggatct caagtcgcgg aaataattcc
    27001 atcatgagga agaccgctgt ctgtatccct aaaaaagcat aggtccataa cggtcgtctt
    27061 aaccatgcac gcaattttat ttgctgttga taattcatga aaacctccca tttcatttta
    27121 gtcaataaac aattgttgga tcttctgatc aaagctctcc ggttgccatt gttcattcaa
    27181 ttgttcacga aaaacaaggc tacatgtttt tttatgaaaa ttatttaaat accggtcgta
    27241 ataaccaccg ccaaaaccga ttcgataacc ggctctattg tagataatac ctggtacaat
    27301 gaggagatcg atcatctccg gcaaatagag cttctctgat tgtggctcaa gaacaccaaa
    27361 actcgaacgt acaagaacgg actgttcatc catttcataa aacgccatct gacgtttcgg
    27421 caaagtttta ggtacaacta ttttcttttg ttctttttct gctcgttgga taaccggctg
    27481 tgtatccagt tcaaattcat tcgataaagt cgtccctatc acttttgctg acttccagta
    27541 ctcgctggcg aatagctgtt tatagattcg ctgttctttt tcctttttct tgccactttt
    27601 ggccagattt ttcagctttt cgattccttt ccggcgtaat cttactttca attggtccaa
    27661 aaactcaccc ttccttctct cataaaatgg tataggaaaa aaagaaaaat agcaatcgaa
    27721 ctttcaaata aatgcataaa tagaataaaa agaagaccta gaaatgattg gccttccttt
    27781 tttaatgaat ttcaactgtt cagcgcgtgt ttttgtctga taaaaagtgc atacagaaaa
    27841 tatggcgcac caaaaagggc aatcaaaatt cctgtaggta cttccccact tggtagaatc
    27901 actctagcaa ccatatctgc actaagcaga aaaatggaac cgaacaaacc gctcagaaca
    27961 aaggtccagt tattttcttt acgtacgact agcttcgcag catgaggagc tagtagtcct
    28021 acaaaagata aactacctgt caccgctaca ctaccgcttg ccaaagcaac agctaacaac
    28081 aaaaggattt tttgactttt agccacgtta aggcctaatc cttgcgcgcg ttcttgacca
    28141 aatgatagca atacaatcct ctgtgcataa agtcctccta gcggaattaa gataaccgcc
    28201 catggaagca atgcccaaac ataaggccag ctcgcacccc agattgatcc cgctaaccat
    28261 gcattcacaa atccataatt ttccttagat aattggatcg tgcatagcaa tgttaatgca
    28321 gaaaggccgg cattaacggc aataccggac aataagattc gattcatacc caaaaagcca
    28381 tttcgatcat aggccgcaag atagacacag caagcagcta aaacaccacc tagaaaggct
    28441 acaactagca atagaaacgg cagcgaatag tgcgaaaaga aacctaaata aagcatcact
    28501 aaaaatcctg ctccggcatt aatgcccaat atactagaat ctgccaattc atttcttgtt
    28561 actccttgca agatataacc agaaacgcca agacaagctc ctgctaataa actgatcagt
    28621 aatcgaggaa ctcgaaattc tctaacaatc agcgccaaag aagatgattc tttccctatg
    28681 aacgtggcaa aaagctgatc caggtaaata aaaggcgtac caatcattaa actacctata
    28741 gacaggatga caagtacaaa aagcaagaaa tagaacgtcc aatatatttt tttcaccgtt
    28801 gttccctcct tacttgtatc agtaaaaacg gaacgccaat caatgcaatg actaatccaa
    28861 aaggtgtttc aaaaggggga ttgatcatcc tcgctgtaaa atccgccaac ataaccaatc
    28921 caccgccaac taaaaaagag acaggtagaa cataacgata gtccgttcca accattccgc
    28981 gaacaactgg cggaaccaac agcccaataa atgaaacagg tccgactaac gaaacggcaa
    29041 tggctgctaa cactgcaact agacagaaaa caatcaaccg cgttcgttta ggatgacttc
    29101 caagagctaa tgcatgtgtt tcactcaaat gaatcaaaga tattgctttt cctaatgaaa
    29161 aagaaaccaa aacagcaagt atatataaag gaaacaatac tgctaattgt ttccatgtga
    29221 tattggcagc tccgcctaca aaccaaaaag caagatcctg ctgcagatta aagaattggg
    29281 taagtgcttg gctgatagca gtaaaaaaag aactaatagc tacgccagct aaaatcagct
    29341 gtaaaggatt catgcccatt ttcgaaaact gcaaaaaacc aaatacgagc aaagttacag
    29401 caaaagaacc tgccaatgac attagaaaca cagaaaaatc agaagcttta ggccaaaaag
    29461 caaaactaag ggccattgcc aacgaagcac ccgcgttgat tcctagtaag cctgaatctg
    29521 ctaaaggatt tcttgtcacc ccttgcatca atgcaccgct caatgcaaaa caactcccta
    29581 caagaaatgc tcccgccaca cgaggcaaac gaatatttcg tatgatttgg tgactttgtt
    29641 ttgatctatc aaaatcaaac aacgcctgcc aaaaatctgc tttttccaca gtcgctgcgc
    29701 catttgaaag agcaacaaaa aaaataatca aaagaacaaa acagaccaaa agcaggagtc
    29761 tatttttctt catagtcctc ataattgatc ctcctgctct tttggcaaca aatcataggt
    29821 tagaaaaact ggtttatttg ttcttggaag cgtgacaatc gttgcatcaa tctcaaatat
    29881 ttttttcata cactcttttg tgatcacttc ttcaggtgta ccttctgtga tcaacttacc
    29941 gtttttcatt gctaataaat aatctgaaaa acgtgaagca tgattaatgt catgaatcga
    30001 catcaagatc gttgtttgtt tcatttgatt gatttcagtc aataactgta ggatctctaa
    30061 ttggtgtgcc ggatctaaaa atgttgtcgg ttcatccaag atcaagatat ctgtttcctg
    30121 agcaagcgcc atagcgatcc agacacgctg ggtttgtcca ccagacagcg ttgcgatatc
    30181 ccgcatacga aatccagcta gacctgtagc atccaacgcc cactcaattg cttcatgatc
    30241 ttgcgtgtcc atgccactga acagtttttg ataagggtat cgtccgtaag atactacttc
    30301 ttctactgtc atacctgtag ggtgttcact agtttgtgct aataatgcaa ttttttttgc
    30361 aagttctttt gaactgtatg tagagatatc agagttattg acaatgatct ttcctgattt
    30421 cacaggtagt atacgggtaa aacttttcaa taaagtagat tttccacttc catttggccc
    30481 aatcaaacta gtgattttcc cttcaggaat cgtaacagac atgttttcaa tgattttttt
    30541 gttttcgtag ccggctgcaa tgtcagctgc tcttaatctc gtcatttcac ccatcctttc
    30601 gctgaaaatc attctcaatt atgacgctgt ttcaacattt cgtcaagcgc tactttcata
    30661 tctatagcca aatgaagcgg ttgacagcgt tcgaaatatc aggaataata accaatgaga
    30721 aagattctca attatgttag gaggaaagaa atgaaattaa aaacaaaagt attcacatta
    30781 tctagcatat tgctgctggc tggagtactg agcgcttgct ccccctcttc agataaagaa
    30841 acagctacaa ctgccaattc gacgacttca tccaatagta ctcatacagt cacagataca
    30901 cttggacata aagtagagat tccaaacgcg cccaaacgga tcatcggcag ttacttagaa
    30961 gactatctga ttgcactagg ggaaaaacct atcgcccagt ggactgttgg cagtggttcc
    31021 attcaagatt atctgcaaga tgatttaaag gatgtcccaa cgatttctta tgaccttcct
    31081 tatgagaaag tgttaagctt tgagcctgat ttgctgttga tttcttcttc cgctactgtc
    31141 gaaggcggga aatacgaaca atatagcaaa atcgcgccaa cttatgttgt aaaaaacgga
    31201 acagatgtca catgggaaga acaactaaaa gatgtcggca aagctttggc aaaagaaaaa
    31261 gaggcagaaa aaatcatttc cgattatcaa aaatccatca agaaaactcg agaagaacta
    31321 gcagataaaa tcgagaacaa gacagctgca gtgttgtggg tgaccaacaa ctcagccttt
    31381 atggtcagca aaaatcggtc aagtggaaga atcgtatacg acgatctgga atttggtgtt
    31441 cctgatctag tggaagaggt ctccaaaaaa gccacttctg actggtctgc tgtttcctct
    31501 gaaaagctag ctgaattaga tgctgactac atcatccttg tgaacagtga caaagatgct
    31561 gccatgttca atgaaccaag ctggaagaat ctaaaagcag tgacagacaa tcatgtactg
    31621 caattcggac ctgaatctag ttggctatat aacggaccta ttgccagtca ggaaatggtc
    31681 aatgatatca aagaaaaact aaattgattt taactaaaaa aagcatcttc acgattagtc
    31741 gtgagatgct ttttttctta ttaacaaact agacgcatag attttattct tcttttgaag
    31801 caaccctatt ttttactctt tcccattttt gaaaacgatg ggcatataga ttttcttgat
    31861 ccgttatccc ctcatactcc tctataaggg cgaagtctga aaaatcaata ttcccaatat
    31921 acgtatcgcc ttcaaacgtg ctatcgatca atgttcgcca gatccgacca gtgtaaggaa
    31981 gaagttcttg aaaaatccga ctaccacccg aaacatagat atcttctgag atcgtttgac
    32041 tatacgacaa aagttcatcg atcgaatgca ttacttccgc attttctttc gtgcgaaaat
    32101 ttggttgcgt tgtcaacacg atgatatggc gataaggaag tgaaagatcc cccattcctt
    32161 cataggtctt tcttcccatc actaaaattc gatccattgt atgttcgcga aagaaacgca
    32221 tatcatttgg caatcgccat ggtaacaaac catttttgcc gatcagacca tttttatctt
    32281 gtgcccacat ggaaataaac atattatccg ctccttatga ttttagatgt tttgagataa
    32341 aaaaagtctc aattctttga ccagcaagga attgagaact ttaaaaacat tattttgttt
    32401 cacggtgcaa agtaactttg cgttcgcgtg ggcaatattt ttgtttttcc aaacgatcag
    32461 gattgttacg tttgtttttg cttgttagat agttacgttc tttacaagaa gtacactcta
    32521 aagtgatgtt tacgcgcatg tttttccctc ccatttttac aagaatcctc taagatgact
    32581 ctcagacttg attatcttac catgattttt tctagattgc tagtgttttt cacgattttg
    32641 ttaagtaaaa ctacttgacg ataaatacaa cggttttact tctcaaacaa aatctttttc
    32701 tttttgtata aataccctta cacttgttta tatagattat gttttttaat aaagggggat
    32761 actaatatgc catggaacat gaaggacttt cctgcatcaa tgaaaaactt agataaattg
    32821 acaagaaaaa aagcaatcga catcgcaaat gcattattag atgaagggta ccctgataac
    32881 cgtgcgatcc caattgccat tgatcaagca aaaaaatggt atgaagatgc tagtgaatca
    32941 gaaaaacgaa ctttcgaaaa agaaaaaaat ccaagcaaga cggatgaaca cgatacgaat
    33001 cctcgtgctg gaaaactgct tgattcagat gtgatcgtag aatatgagga agaacaatgg
    33061 atcgtgaaat ctaaaggggc aaaaaaggca agcaaccatt ttgatacaaa aaaagaagca
    33121 atcgaaaaag gaaagcaagt agcacaaaac aaagaatcaa ctttgttcat ttataaaaaa
    33181 gatgggacaa aagaaaaaga aatcgattat tgaacatatg taaaggggtt gtgacgaaaa
    33241 tcgtgtcaca acccccttat gttcgaaaca atggtgttcc tctgccaact ccgaaccata
    33301 agtcataata tctataaaac gggaagagct gttttttgaa attgtttctt atcactcagt
    33361 gttaaacggt aggagaaaac ctctttttta ttgatatttg attttcatca aacagtcaca
    33421 tagtcgattg cttctgcttg cggaatcgtt tcatctacaa agtagacttt ataccatgtc
    33481 aagaaactga agattgtcca gatttttctt tgctcttctt tacgtccttc gtgatggtca
    33541 tctagcaact tcatgatttt ttcttgatca aaaaattctt ttgcaaaatc tgcttcgaat
    33601 aatgctttga cttgttcgta gccgtgttct tctttcaacc aagctttgat tggcacaggg
    33661 aatcctaatt tcacacgatt cgaccattct tccggaagat gtttgttcgc agcttgtctg
    33721 aaaatatctt ttgtgttgtt ttgattcaat aaaaacttac ttggtatttg ttgagccaat
    33781 ttcatgactt cgatatccaa taaaggtact cggacttcca aagaacttgc catagaaagt
    33841 ttatctgcct ttaacaagat atcttttggc atccattgat gaagatcgac atattgcatc
    33901 ttattgactt catctttgat tccttctgtt ttctcgtaat gaacagtcat gatttcatcc
    33961 acagatggtg ctgtttgata ttttggtgtt aacacttctg atgcttctga ttcttcgaac
    34021 actcgagcat gaccgataaa atattctttt gcaggagcaa gtgattcata taaatggatc
    34081 cgtccatgga agtttttcat ttttccgatt ttacgtccta aattatagcg tgttcctttt
    34141 ggtaattttt tcaatccttg agcaatcaca cggatgaatt ttgaattcgt atggaatcca
    34201 tacgtttggt atcccgcaaa cagttcatcc gccccttctc cagattggac tacccgcaca
    34261 ctttgtgaag caagatttgc taaaaagtac aaaggtacgc aagaaggatt tgaatcaggt
    34321 tcatctaagt gatactggat caaagggaat gctttgaacg acatgtctcc gtcgatcact
    34381 gctgctgtat tatctaagtc caatagttcg gttagttttc tggcttcaat cgcttcatta
    34441 taggttttat catcaaaccc gattgagaaa gaatgatctg gtttcaatac agaggttaca
    34501 taactggaat caaccccgct ggataagaaa gaaccaactt ctacatcact gaccgtatgt
    34561 gcttcaatag aagcttgaac ggtttcgtcg attttttcga tccattcttg tcgggaatgc
    34621 gtttcttttg tttcaaaatc tgcatcccaa tattgctgaa tgtctatttt tccatcttta
    34681 tacgtataat aatggccttc aggcaaacga tagacatctt tgaagaatgt ttcgccgttt
    34741 aatggagaat attggaaagt catataaggt ttcaacgctt ctttgttcaa ttctttgtta
    34801 aaatcaggat gaggcagaaa acttttgatt tctgaaccaa acataaatgt tccgttcatt
    34861 tctgcataat aatatggttt gatcccaaag tggtctcttg caccgaataa ctcattcttt
    34921 tcattatccc aaatagcaaa agcaaacatt ccgcggatct tttgcaataa ttctgttccc
    34981 cattcttcgt aaccatgcaa aaggacttct gtatctgcat gtgtttgaaa aacgtggcca
    35041 gctgcaatca attcttctct caaaggctgg taattataga tttcgccatt aaaaataatg
    35101 atctttgtcc gatcttcatt gtaaataggc tgagtaccgc cctctaagtc aataatactt
    35161 aatcttctga atcctaaagc aacatgttta tcgatatatt ctcctgaact attaggtcca
    35221 cggtgaacga ttcgatccat cattgtatta ataattgttt ttttgttgtc cttgtcgtta
    35281 acaaaaccta caataccgca catttaactt tcacatccta atataaaatt actgtttatt
    35341 tcattcatat gcatcttatc atagaataaa tagcggacga atattttctc tcttacaatt
    35401 tcgtcacgtg aaaacaaaag atgaaagttt ctgtttttac agaaaataaa gtcccaatat
    35461 ctaatttacc atgttttctt ttttgaggct accttattct cttcgtgtta aaaaaatatt
    35521 agtggatgct atttccaaaa aaaccagcaa aaaaagacac gacctaaaga tcgtgt
//
LOCUS       DACDTH010000025        35237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDTH010000025 DACDTH010000000
VERSION     DACDTH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.025"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(219..704)
                     /locus_tag="IWK54_001290"
     CDS             complement(219..704)
                     /locus_tag="IWK54_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9593419.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1184..1825
                     /locus_tag="IWK54_001291"
     CDS             1184..1825
                     /locus_tag="IWK54_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9593420.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1844..2479
                     /locus_tag="IWK54_001292"
     CDS             1844..2479
                     /locus_tag="IWK54_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9593421.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2497..3312
                     /locus_tag="IWK54_001293"
     CDS             2497..3312
                     /locus_tag="IWK54_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9593422.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3631..5067
                     /locus_tag="IWK54_001294"
     CDS             3631..5067
                     /locus_tag="IWK54_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9593423.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5256..7703
                     /locus_tag="IWK54_001295"
     CDS             5256..7703
                     /locus_tag="IWK54_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9593424.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7933..9000
                     /gene="rlmN"
                     /locus_tag="IWK54_001296"
     CDS             7933..9000
                     /gene="rlmN"
                     /locus_tag="IWK54_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9593425.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9193..10296)
                     /locus_tag="IWK54_001297"
     CDS             complement(9193..10296)
                     /locus_tag="IWK54_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9593426.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10849..11397
                     /gene="aac(6')"
                     /locus_tag="IWK54_001298"
     CDS             10849..11397
                     /gene="aac(6')"
                     /locus_tag="IWK54_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9593427.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            11943..13604
                     /locus_tag="IWK54_001299"
     CDS             11943..13604
                     /locus_tag="IWK54_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9593428.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(13674..14021)
                     /locus_tag="IWK54_001300"
     CDS             complement(13674..14021)
                     /locus_tag="IWK54_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9593429.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            14105..15046
                     /locus_tag="IWK54_001301"
     CDS             14105..15046
                     /locus_tag="IWK54_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9593430.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            15047..16090
                     /locus_tag="IWK54_001302"
     CDS             15047..16090
                     /locus_tag="IWK54_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9593431.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            16096..17166
                     /locus_tag="IWK54_001303"
     CDS             16096..17166
                     /locus_tag="IWK54_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9593432.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            17156..18109
                     /locus_tag="IWK54_001304"
     CDS             17156..18109
                     /locus_tag="IWK54_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9593433.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            18121..19131
                     /locus_tag="IWK54_001305"
     CDS             18121..19131
                     /locus_tag="IWK54_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9593434.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(19416..20237)
                     /locus_tag="IWK54_001306"
     CDS             complement(19416..20237)
                     /locus_tag="IWK54_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9593435.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(20334..20612)
                     /locus_tag="IWK54_001307"
     CDS             complement(20334..20612)
                     /locus_tag="IWK54_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593436.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            20880..22889
                     /gene="metG"
                     /locus_tag="IWK54_001308"
     CDS             20880..22889
                     /gene="metG"
                     /locus_tag="IWK54_001308"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9593437.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(23368..23955)
                     /locus_tag="IWK54_001309"
     CDS             complement(23368..23955)
                     /locus_tag="IWK54_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593438.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            24130..25452
                     /locus_tag="IWK54_001310"
     CDS             24130..25452
                     /locus_tag="IWK54_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9593439.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            25607..26383
                     /locus_tag="IWK54_001311"
     CDS             25607..26383
                     /locus_tag="IWK54_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9593440.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            26376..26945
                     /gene="rnmV"
                     /locus_tag="IWK54_001312"
     CDS             26376..26945
                     /gene="rnmV"
                     /locus_tag="IWK54_001312"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9593441.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            26938..27822
                     /gene="rsmA"
                     /locus_tag="IWK54_001313"
     CDS             26938..27822
                     /gene="rsmA"
                     /locus_tag="IWK54_001313"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9593442.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            28273..28902
                     /locus_tag="IWK54_001314"
     CDS             28273..28902
                     /locus_tag="IWK54_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593443.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(29026..30135)
                     /gene="ugpC"
                     /locus_tag="IWK54_001315"
     CDS             complement(29026..30135)
                     /gene="ugpC"
                     /locus_tag="IWK54_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9593444.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            30344..30766
                     /gene="mgsA"
                     /locus_tag="IWK54_001316"
     CDS             30344..30766
                     /gene="mgsA"
                     /locus_tag="IWK54_001316"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9593445.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            31089..31625
                     /locus_tag="IWK54_001317"
     CDS             31089..31625
                     /locus_tag="IWK54_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9593446.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(31665..33428)
                     /locus_tag="IWK54_001318"
     CDS             complement(31665..33428)
                     /locus_tag="IWK54_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9593447.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(33431..35146)
                     /locus_tag="IWK54_001319"
     CDS             complement(33431..35146)
                     /locus_tag="IWK54_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9593448.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT        11465 a         6280 c         7302 g        10190 t
ORIGIN      
        1 cgatttataa tcgaaaaaag aagcattttt atatatgctt cttttttcgt agaatcaata
       61 aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt tcatactagt
      121 tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa ataaagaaaa
      181 agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc ataaactggc
      241 agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat caacgtagtg
      301 gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc atcgatcacg
      361 acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt acctgctgga
      421 acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt ttcttttaat
      481 tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac aagatagatt
      541 tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg tagggaaaaa
      601 gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga gatacccagt
      661 ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct
      721 ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat gtaaattaca
      781 cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt tttagagagt
      841 ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag tcgattttct
      901 gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg ctagatgacc
      961 agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg
     1021 tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac gactgtgtga
     1081 ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac gtcctcttgg
     1141 catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt atttgattga
     1201 agttatgccg caattattag taggagcact cacaacattg aaattatttt tatttacctt
     1261 gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc attttaagcc
     1321 gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc tgttgttgca
     1381 actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc gtttcgaagc
     1441 agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct ttcgtggagg
     1501 gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat taagtccctt
     1561 tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac cttctgtagg
     1621 gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag gtttaggtga
     1681 cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc ctttgctagg
     1741 cgtggcaatc atctatctta cattgactgg tattctaaca atcataatga aacaagttga
     1801 aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg aattgaagaa
     1861 agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga
     1921 tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc ttttacgtat
     1981 cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg taccgttcaa
     2041 cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag acttccagct
     2101 atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg tattgaaaca
     2161 gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag ggattagtga
     2221 tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg tagcgattgc
     2281 cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa gtgctttaga
     2341 tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag atggggtaac
     2401 ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc aattactaaa
     2461 agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag ttttctattc
     2521 gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac ttcagcagat
     2581 aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga tacatttgtt
     2641 cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga tttagccaaa
     2701 gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg gtcaatgaag
     2761 gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc caaaacgccg
     2821 gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca agtgctgatc
     2881 acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag attaggtgcg
     2941 caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt aaaagagata
     3001 gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat cgatctgaaa
     3061 agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta tttgaaacaa
     3121 agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga ggattttgca
     3181 gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa
     3241 ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga agatgttacg
     3301 ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc tttttccgaa
     3361 taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg ctcacaacct
     3421 cttggtaaac ggtgttcaaa agccagtttt tcggtattaa gccaaattta tgcaaaaatg
     3481 tagaaagcca ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac
     3541 ctcctttaca taattttatt ttgttgtgga aggggtttcg tgttattcta aaagaggaac
     3601 gattgaaggt tataggaaag gaagagatga atggaacaca aagaacttac ctcatttcca
     3661 aaagattttc tgtggggctc tgcttctgca gcgtatcaag tagaaggagc ttggcaagaa
     3721 gatgggaaag gagaatcagt ctgggatcga tttgtacgga tacctggaaa gacatttaaa
     3781 ggaacgaacg gtgatttggc agtagaccac tatcatcgtt ataaagaaga tattgcttta
     3841 atgaaagaac aaggtctgaa agcttaccgc ttctctgtag cttggacacg gatttttcct
     3901 aatggacgag gagaagtcaa tcaagctggg ctcgcttttt atgagagatt gatcgacgaa
     3961 ttgattgaaa acgatattga acctatgttg actttatatc attgggatct tccacaagca
     4021 ttgcaagatg aatataatgg ttgggaatca cgtcagatca ttgaagattt cactcattat
     4081 gcagaaacat tatttgaggc attccgcggg aaagtccatt actggattag tttgaatgaa
     4141 caaaatatct tcacttcatt aggttatttg ttagcagcac atccgccagg tgtgacagac
     4201 ccgaaacgaa tgtatgaagt taaccatatt gctaatttag caaatgcatc tgtcatcaat
     4261 aaatttcacg agttagaaat gcctggaaaa atcggaccaa gctttgcgta tacgccaaat
     4321 tatccaattg acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga agacctgatg
     4381 gccaattatt ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc catgaggttc
     4441 ttagaagaaa aaggatgggc accaacgatt gaagctggcg atgcagaatt gctagaatct
     4501 gctaaaccag attttcttgg gatcaattac tatcaaacgg cgacgaatgc attcaatcca
     4561 ttaaacggag ttggtgctgg aaaaatgaat acgacaggaa aaaaaggttc atcagaagaa
     4621 actggagttc ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg aacaaattgg
     4681 gactgggaga ttgacccaga aggattaagg atcggactgc gtcgtatcac aagccgttac
     4741 cgcatgccgg tactgattac ggaaaatggc ttaggagagt atgacaagtt gacagaagat
     4801 aaacaaatcc atgacgacta tcgaatcaat tacttacaaa gccacgtcaa agccatcaaa
     4861 gaagcaattt cggatggagc agaagtccta ggctattgca cctggtctta tacagatttg
     4921 cttagctggc tgaacggtta tcaaaagcga tatggatttg tatatgttga tcaagacgaa
     4981 acacaagaag gatctttgga acgatataag aaagacagtt attactggta ccaaaaactg
     5041 attgaagaaa atggagagaa cctttagttc acatacttaa aacaagaagg tacggctgga
     5101 tatggccgtg ccttttcttg ataaaatcat ttagaaatta agaaaaactg taaaataagc
     5161 tgaaaaatga aagaaagcgt cagttctttc aaacaaaact ttagttccct tacttttttt
     5221 tgtataatga aacttgatga aatgagggat aataaatggc acaaaagaaa cggcctacta
     5281 aaaaaaggaa aaagacgaaa aaaacgaaaa agcaaaaaca gcagcaggaa caaatgctga
     5341 cgattggtgt tgggtttgcc ttgattttat ttgctgtctt cggcttttta aaattaggtt
     5401 ttttaggaat attgattgca aacggctttc ggatcattgc aggaaatact taccaaatcc
     5461 tctgtctttt attagccgta ttaggatttt ggattgtcat taagaataca gagttttcaa
     5521 ttggtaaaaa ccgacgctgg tttggcggca tactctttta tttcggtatc ttattattgt
     5581 tacatgccca tttatttggc aaactccata ctggggaacc aaatatcatg gggacgacat
     5641 gggatttgtt agccagtgat ataaaacaaa gtcaagtaga caataacgtt ggtggtggga
     5701 tgattggagc aatcctttat cattttactt acttcttgat tgcacagcct ggaagttatt
     5761 tagcagcagt gctattactg gcaggaggag cattcctgtt cagtaatctg gaaggctatc
     5821 agctgttaaa tggtatccaa tcaattggag aacgagtaca ggaattattg gaaggtgatc
     5881 ctgccaagca agctcgaaag caagcggcaa aggaagagag aatgaaacag cgcgcagaag
     5941 cgaaagaagc aaggcgctta gctgcaaaag aagctgcaga aaaagaagct gcagaatatg
     6001 agaagaaaaa agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg caggaaccag
     6061 aaaatacaga gccggagcag ctaagttttg ttccaatcga cagttttcaa gaaaatatcc
     6121 atcctgcgaa tctagagaaa ccagtaccag atacaccaaa acagaccaat acagcagaag
     6181 gtttttcaga tgagttgcca gaggatgacg gtacctcatt agaatttgag attgaagcag
     6241 aacaggaaaa tcaagactat gaattaccga cagttgattt gcttgattct ataccaactg
     6301 tggatcaaag tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt
     6361 tccaaagttt cggtgtcgat gccaaggtcg tcaaagccag cttaggacca tccgtcacaa
     6421 aattcgaagt ccagccggct gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg
     6481 atatcgcgct tgctttagcg gcgaaagatg tccggatgga agcaccgatt ccaggtaaat
     6541 cactgattgg tattgaagta ccaaatggaa agatcagtat ggtttccttt agagaaatca
     6601 tcgaagcaca accaaaccac ccagataaat tactagaagt acctttagga cgtgatgttt
     6661 ctggacgggt acaaacggct gatctttcga agatgcctca cttactcgtg gctggttcga
     6721 ctggtagtgg gaagtcagta gcaattaatg ggattatcac aagtatatta atgcgtgcta
     6781 agccccatga agtaaaactg atgatgatcg atccgaaaat ggttgaattg aatatgtaca
     6841 acggcatacc tcatctgttg acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct
     6901 tacaaaaagt agtacaagaa atggaagaac gatatgaaaa attcgcggca accggagtaa
     6961 gaaatatttc tggttacaat gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac
     7021 atccaacctt accattcatc gtagtgatcg tagatgaatt agctgatttg atgatggtag
     7081 ccagcaatga agttgaagat gcgattattc gtttagcgca aatggctcgg gcagcgggga
     7141 ttcatatgat cctagctact caacgtccaa gtgtggatgt tatcacagga atcatcaaag
     7201 caaatgtacc ttccagaatg gcatttgcgg tatcaagcgg tacagattct cggacgatca
     7261 ttgattctaa tggggcagaa aagctcttag ggcgaggaga tatgctattc ttgccaatgg
     7321 gtgagaataa accgatccgt gtgcaaggag cattcatttc tgaccacgaa gtagaacggg
     7381 tcgttcaatt tgttacagat cagcaagaag ctcattatga agagaaaatg atgccgacag
     7441 atgaagttga aacgacaggt gctccagaac agccacaaga tgagctgttt gaagaagcaa
     7501 aagcacttgt cgttgaaatg caaacggcaa gtatttccct tctccaaaga agatttcgga
     7561 tcggttacaa ccgagcagca cgtttagtag atgaactgga agctcatgga gttgttggcc
     7621 cgtcagaagg aagcaagcct cggaaagtat tcattgagca ggaagaaaca tcagaaacgt
     7681 tgccggacga taatgacatg taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa
     7741 taaaaagaca aggcatgatg ataccaaaag aaaaaatggt ttttttccat cttttctaat
     7801 acggtatgat gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg
     7861 tgaagtattg cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag
     7921 agggaaaata ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat
     7981 tggtttatag aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat
     8041 caaaaacgtg taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa
     8101 ttatcggagc attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat
     8161 ggtacagtaa aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg
     8221 agacaagaat atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt
     8281 acgttttgtg caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt
     8341 gcacagatca tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca
     8401 catgtcgttg taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt
     8461 ttgcatatca tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc
     8521 actagtggat tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg
     8581 gctatttcct tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga
     8641 agtttcccaa ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt
     8701 cgaatcacgt ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa
     8761 caattagcag atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat
     8821 aatccagtca gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt
     8881 tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat
     8941 attgatgcag cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa
     9001 tcgtatccta ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact
     9061 gttcaactta aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag
     9121 aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt
     9181 tttagcaatt atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata
     9241 agtttctacg tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg
     9301 tttaatatca tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt
     9361 gagattcatg tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt
     9421 atcaaacaaa tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga
     9481 tgctccgtga aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg
     9541 tgccatagca gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat
     9601 gatatccgaa acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa
     9661 tgcttgcgta ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa
     9721 tctatccgtg tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga
     9781 taaagcatcg ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt
     9841 atcaatgaag gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc
     9901 ttcgttatag atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa
     9961 ggtgaagact ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc
    10021 accaccgact gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc
    10081 aatatttcca taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg
    10141 tgtcaaagct acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag
    10201 cactactttt gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc
    10261 aatggtataa tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg
    10321 aaacgggtta aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt
    10381 gaacctaaga caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa
    10441 gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc
    10501 tcttagtatt tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt
    10561 aagattatta tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca
    10621 aaagataatc tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct
    10681 gatctttcct attttgaata aagtgtttct gtataatttt tttattttat aagtaagata
    10741 aataatgaaa gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg
    10801 ttttttcaaa aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt
    10861 gaatttgacc gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact
    10921 tggccggaag aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa
    10981 cgaatcgcgg tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct
    11041 caatacggta tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag
    11101 aaccaaatag gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga
    11161 atcacgattt atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac
    11221 ctgtatgagc atacatttga taaagtggct tccatccaga accttcgtga acatccgtat
    11281 gaattctatg aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg
    11341 gacaaaccgg atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa
    11401 aaagaggttg tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac
    11461 ttcttgaatt tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg
    11521 taccagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
    11581 gctttctaca tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt
    11641 ttattcagat aagggactgt gacaagactt ttgtcacagt ttctttttgt ttattctttt
    11701 ttatctttgt atggcaataa tagacaatat aacgaaaatt atttaatggc ttttggaaga
    11761 ccatggaaaa ccttgtacaa agctattttt tctgaaaaat tccttgaaac ctcacgaaaa
    11821 aatcattatt ttatgaaaaa gacaaaaaaa agtgttgatt ttttaataat tattcattaa
    11881 actcttaaac aacttcagaa aatattaatt attctgaaaa ttccaataaa ggggtacgta
    11941 aaatgaaaaa gaaatttaca tttggtttgg ttgcggtatg cggccttgta cttgctggat
    12001 gttatggcgg cagttcagcc acaagcagta gttcagcaaa aagcggttca gcagatggag
    12061 gaggcgtatt taacttagtc gtgcctcaag aaatgcctac agctgactta tcagttgcaa
    12121 cagatacaat cagttttaca gcattgaata acgtttatga aggaatttac cgtcttgatg
    12181 aagacagcaa accacagccg gcaggagcta gcgagttggc agaagtcagt gaagacggat
    12241 taacttacaa aatcaaatta cgagaagacg caaaatggtc aaatggtgat cctgttacag
    12301 cagcggatta cgtttatggc tggcaacgaa cagtagaccc agctacagct tctgaatatg
    12361 cttacctgta tgcgccagtt gaaaatgcag aagacattac agctggaaag aaagataaat
    12421 cagaactagg gatcaaagca gttggtgact atgaattaga aatcaaattg acgaaacaaa
    12481 caccatattt ccaatatctg cttgctttcc catcattttt cccacaaaaa caatccgtag
    12541 tggaagaaaa tggggatgcc tatgcatctg caagtgataa agctgtttat aatggaccat
    12601 tcacattaga agattttgat ggaccaggaa cagatacaga atggacttat aagaaaaatg
    12661 atcattactg ggacaaagat acagtaaaac tttctgaaat caaagtcagt gtagtaaaag
    12721 aatcttctac tgcattgaac ttattcaaag atggccaagc agatgacgtg atcttgtctg
    12781 gtgagttagc acagcaaaac gcaaacgatc cagcttatac ttctgttaaa gaagcgcgga
    12841 caagctatat cgaattcaat caacgtgaaa agaattcacc attcaacaat gtgaacctaa
    12901 gaaaagctat ttcttattct atcgatcgtg aagctttagt aaaacaagta ctaggtgacg
    12961 gatcagttgt ctctacagga ttgatcccat cagatatggt aaaaagtcca acaacgaacg
    13021 aagactttgc aaaagaagct ggaaaattag tttcttatga caaagataaa gcaaaagaat
    13081 attgggaaaa agcgaaaaaa gaattgggcg ttgattcttt agaatttgat ttgatggctt
    13141 ctgatgatga ttcatcgaaa aaagtaatcg aatatgttca aaactcaatc caagaaaact
    13201 tggatggcgt aacagtcaaa ccaacacctg ttccattctc tgtacgtctt gaccgctcaa
    13261 cttcgggtga ttttgacgca gtactaggcg gatgggctgc tgactatgca gacccaagca
    13321 gctttactga tttgtttgtc acaggtaatt catacaaccg tggtcgctgg agcaatgcag
    13381 aatatgacga agcagttaaa gcagcttcat caacagacgc tggcgatgaa caagcccgtt
    13441 gggatgattt ccaaaaagca atcaagatca tctctgatga tatgggtgtc gttccggtgt
    13501 accaaaaagc agaaggtcac ctagtgaatg aaaaaatcaa aggtatcgtt caccatgcgg
    13561 caggcgcttc atgggattac aaatggacat acgttgaaga ataatcgtta ataccgtgag
    13621 catgagaaaa aacgaggctg cggcaaatgt cgcaacctcg ttctcatatt cacttatcct
    13681 aacaaagaaa aaagcatgaa aatagcagaa agagcaatta gtattccggc aatgatcagc
    13741 caaaagctgt acataccgta gctggcagac gagagcgagg aaaattctgg ttttttcttt
    13801 cggttccttg ttcgtgcaag atggaccgat cgctgaaaat gatcgaagaa acccgaagaa
    13861 aagacccata aaaaaccgcc gataatcaga aacggcaatg accataagaa tagatcatta
    13921 gataattgaa tgagtgtaaa ggaatggttg atgagatttt ttattaaaat aaccagaatc
    13981 agtagaaaag ctactgcgta ggggatagat ttttttttca tttgattgct cctttatttt
    14041 tggttcatta tctaataatg tagcgaaaca tctgtcagaa gtcaaaagct agggggaaac
    14101 attagtgaat agttatatca aatatgtatt aaaacgtgtc tttttcatga tcatcacgct
    14161 ttggttaatt gcaacaatca cctttttctt gatgcaattg ctgccgggca cgccatatac
    14221 gaatcaggaa cgtttgagtc ctgaaacaat cgagatgcta aataaacaag tcggtttgga
    14281 taaaccagtt atcgtacaat atgggattta tctttctaat ttgcttcaag gagattttgg
    14341 aatttccttc caatttaaaa accagccagt cgcacatttg ttagctggaa gaatcggacc
    14401 atccttgcag ttagggttac aggcaattat ctttggaaca gtcctaggga cgattttagg
    14461 aacgatctct gccatgaagc aaaatacatg ggcagataca tcttctacac tcgtagcgat
    14521 attaggtcgt tccattccaa actttgtatt tgctgtttta ttacaatata tctttgcaat
    14581 caaattacaa gttttgccaa tcgcaaaatg ggatggcttt gtgtacacaa tcttaccaac
    14641 aattgcgttg gccatgtcgc cgcttgctga ctctgctcgg tttatccgta cggaaatggt
    14701 ggaagtactg catagtgatt atgtggaatt agcaaaagca aaaggattga gccgttggga
    14761 aatcgccttt aaacatggtc ttcgaaatag tttgattcca ttgatgactt tattaggacc
    14821 gttagctgta gcattgatga ccggatcact ggtggtagaa aatatctttg ctattcctgg
    14881 tatcggggaa caattcgtta agtcaatcac aacaaatgat tatcctacga tcatggctgt
    14941 aacgatcctt tactcattta tgctgatttt tgttattttg atcgtggatc tattgtacgg
    15001 tctagttgat ccaagaattc gtgtttcaga agggagccga ggataaatgg aaatgatacc
    15061 aaagcgttat gaaacagttg cagatatccc tgcggatgaa tttcttccat tacaaaaaaa
    15121 taccgaagaa gaacgtgaac agattactgc accttctttg aattttatcc aagattcatg
    15181 gcgccgttta aagaaaaaca aagctgccgt tatttcaatg gttttactat tggtcatcat
    15241 tttgatctct gtgatcacaa tctttgtttc tcctcatgat ccaactgccc aaaacgtggc
    15301 ttatatcaat ttgcctcctc gtattccggg aatcaatatc gatggattga atggaaaagc
    15361 aatggttggt ggtgaacttg ttgataaata tgctcaagca aacgtccctt caaacgtaaa
    15421 tttcttttta ggaacagacg gtttgggacg tgatgtatta agccgcttat ttatgggaat
    15481 gagaatctcg ttgctgatcg catttattgc tgcattacta gatgtaacga ttggtgtagc
    15541 ttacgggttg gtttctggtt tgttaggcgg acgtgtcgat aacgtgatgc aacgtttttt
    15601 ggaaatctta tcaggaattc caaacctcgt tgtcatgatt ttgatgttgg tcgtttttga
    15661 accaggaatt ttttcaatcg tggctgctat ggccatcaca aactggatac caatggcacg
    15721 tatcgttcgt gcacaaacaa tgaaattaaa agatcaagaa tatgttttag ctggattgac
    15781 attaggtgaa tcaagaatga aaattgcatt caaacatatt ttgccgaata tctctagtgt
    15841 cattattatt caaatgatgt ttagtattcc atctgctatt ttcttcgaag cctttttaag
    15901 ctttatcggt ttaggtctta cgcctccgtc agcttcatta ggaacgatgt tgagcgaagg
    15961 gtacaagaca ttcctgtatc tgccttatct attatggatt ccggcagtaa ctttatcggt
    16021 tatcatgatt ggctttaact tacttgctga tggattgcgt gatgcctttg atcctaaaat
    16081 gaaagagtga agacgatgac gaaaatttta gaagtgaaag acttagaaat ctcttttgat
    16141 acgtatgctg gaaaggtacg agcgattcgc ggtgtcaatt ttcatttgaa taaaggagaa
    16201 acattggcga tcgttgggga atctggaagt ggaaaatcag tgacgaccag aagcatcatg
    16261 cgcttgctgt caagcaacgc caatatagat gctggtcaaa tcctctttaa aggtaacgat
    16321 atcgttggaa aatcagaaag agagatgcaa aaaatccgtg gtaaagaaat agcgatgatt
    16381 ttccaagatc caatgacctc tttagatccg acgatgccca ttgggaagca agtcgctgag
    16441 tcccttagaa agcataataa agtttcaaaa aaagagggac aaaaagctgc attagaattg
    16501 cttaaattag ttggaatacc cgaagctgag aaacgtatca acagttatcc gcatcagttc
    16561 tcaggaggtc agcgtcaacg gatcgtgatc gcgattgcac tggtatgtta tccggaaatc
    16621 ctgattgctg atgaacctac aacagcgctt gatgtgacta ttcaagcaca gattttggag
    16681 ctgctgaaag aaattcagac aaaaatcgat acatcaatta tctttatcac tcatgacctt
    16741 ggtgtagtag cgaatgtcgc tgatcgtgtc gctgtcatgt atggaggaag aatcgttgaa
    16801 gttggaacat cggaagaaat tttctacaac ccgcaacatc cctatacttg gggactgctt
    16861 ggttcaatgc cgacattaga tagtgcgaac gatcgattgt atgctattcc tggttctcca
    16921 ccggatttat tgaacccgcc aaaaggagac gcattctatc caagaaatga attcgcgatg
    16981 aaaatcgatg cagaacaaga accaccattt tttgaactat caaaaacaca caaggcagca
    17041 acttggctac ttgctccgca ggcacccaaa gtgacaccgc cagaagaaat ccaacgtcgc
    17101 tgggctatct ttaaagagaa acaaaaacat acttacggtc aaggaggaat tgctgatgcc
    17161 aaataaacta cttgaagtta aaggattgaa acagtatttc aatgtaggaa aaaagaatga
    17221 agtacatgct gtaaatgaca tcagctttca catttatgaa ggagaaacat tcggtttagt
    17281 tggagaatct ggaagcggaa aatccactac tggccgtacg atcattcgct tgaatgaacc
    17341 tactgatggt gagatattat tcgacggtca agatataacg aagatcaaag ataaaaaagg
    17401 tttgacgaaa ttccggcatg atgttcagat gatctttcaa gatccatacg cttcgttgaa
    17461 tcctcgtatg aaagtcagag atatcattgc cgaaggaatt gatgttaatg gactagctaa
    17521 atcatctgaa gagcgtgcaa aaaaagtcga tgatctattg agaactgttg gattgaatcc
    17581 aagccacggt actcgttatc ctcatgaatt ttcaggtggg caacgccagc gtattggtat
    17641 tgcacgagca ttggcagtaa aaccgcgttt tatcatttgt gatgaaccaa tttctgcact
    17701 agatgtgtcg atccaagccc aagtggtcaa tttgttgcaa gatctccaaa aagaacatca
    17761 attgacctat ctatttattg cacatgattt gtcgatggtt aaacatatta gtgaccgaat
    17821 cggtgtaatg cataacggct tgttattaga gatgggaaca agtgatgaaa tctataatca
    17881 cggtgttcat ccttatacag aaagtttgtt atctgctatt cctctaccag atccagacca
    17941 tgagagaaaa cgcagaagaa tcaaatatca acctgaacca gatgacggac aagtacgtca
    18001 acttcgtgaa atcgcaccag aacattttgt ctatgcaact gagcaggaag tcccatatta
    18061 cgcaaaaaaa ctaaaacgac aaaaggaatc tcttctggtt gctaactagg agggacatag
    18121 atgctaaaag cttttaaatt ccgtatgtat ccgacagaag aacaaaaaca gcaattgatt
    18181 cgtacttttg gatgtgctcg atttacctat aaccatttgc tgaaaaagcg gcagaaaagt
    18241 tggcaacaaa caggagtagc caatttttcg ctcacacccg ctacattaaa aaaagaatac
    18301 ccattcttaa aagaagtaga cagtcttgct ttagcaaatg cacagcttaa tttggatcgg
    18361 gcttttcgca attatttcaa gggacgtgcc agcttcccaa agcttaaaac gaaaaaaagt
    18421 atgtggcaat cttatacgac caacaaccaa acccgtacga tctatctgga aaatggctac
    18481 ttaaagttac caaaacaaaa agaactaatc aagataaatc agcatcggcc agttgaagga
    18541 tcgattcgct ccgctacgat ttctgctcga tataatgaag aattttatgt cgcactactt
    18601 tgtgatgttt cgccagtaaa aaaagaatct ttagctaaat ggatcgggat cgcctatcat
    18661 ccgaagacat tgatccaaac atctcgacct ctagaagtaa ctttaccaaa atttcaccag
    18721 acagaagaaa agctgcaaca tgcacagcgg aaattgaatg tgaaagtccg ctccgctcac
    18781 catagaaaga tcaggttaga tcaggcaagc aattatcaga aacaaaagag aaaagtaatg
    18841 gatctttatc tgaaacaaaa gaaccagcga gaagattatc ttgaacaatt atctgggaaa
    18901 ttggtcaaac aatatgacta cttatttgta gaatcttttc caaaagaaga ggcacatgct
    18961 gatttttcta ttcacgactg gcataaatta ataacaaaat tgcgttataa gtcccaatgg
    19021 tataataaaa aatttctttt gatcaatacc gacggagcag aagaaagcaa ttccgtaaga
    19081 aagagccaag tagtagaaaa aatgggcaga cattcggtta tcaagggata aaaaatgaat
    19141 gaaactgata ggagcgttag ggttagcctt gttaataaaa agtcttaatg acttttgttt
    19201 ctaggaattt ttcttttgaa gaaaagctca ctaattatga caaaccaata aaacaatagc
    19261 agaaaaagtt cgtacgagct ttttctgcta ttgttaattt tttattcaga aagtttacga
    19321 atacatcaaa attctgaaac aaaaaacgta actcacaaag agttgttttt gcgttttttc
    19381 ttgaatgatt aggtctgaat cttttgctca caaccttatt catggtgccc cggcttgagc
    19441 tgatagattt ttgaaggacg gccgattcct acaggacgtt caccgatctc atcgaaatat
    19501 tggagcatag ctttcttaaa attggaatga tcgatcgttt tataatcgat ccctaagaat
    19561 ttagcaaata cttttcgagc ttccgtaatc gtgaaatctt tacctaaaac ttgtagaact
    19621 tgcggctcgt gttccatttt attgactaca cggttgaatg ctttcaagat gatttgacta
    19681 tggtcaaaag ccaaagtatc tttgccagaa gactctccag tgactaaatc aagcgagata
    19741 gccacttctc cgctagataa atggatcttg ttactatgac gttctagtgt gaaccagcgt
    19801 acttccttgg catcatctcc ggcgattagc ggttcttctc caataaaggc taagtagctg
    19861 actgtgacga cccagcctcg cggatctcga ttaggcgtac tgaatgtatg cagttgctca
    19921 atattttctt ttgaaataac cacaccagtt tcttcctttg tttctcttaa gacgctttct
    19981 ccagttgact catccttttg gacaaagcca ccaggcaatg cccatgaatt tcgataagga
    20041 tgcccttttc tttggatcaa caaaactttc agctgatctt cttctttgtt atagcacatc
    20101 agtacgatat cgacagtcaa agagggcttg tcatattctg gacgttcctg tttttgatac
    20161 cagttaagaa attcttcttc acttgcttta tgttcataat ataatttttc ttcttcttta
    20221 gaagcaaaac ggttcatctg accacctcca ttgacagtaa aagtactttt taccttattg
    20281 tagcagactc tgaaaaaaaa ccaaggattt acttgaaaaa tctaggttat catttaaata
    20341 tgaaaaataa atggaagtat gagattgaat aaaaagaaaa gcagataaaa cggcaatact
    20401 ttataccgct cttcacgaat ggcaaagaag ccacaaataa cgatatatac gtcaatgata
    20461 aaacgcaaaa tccaagctgc tgtcataaaa ctgttcaaat gcccatggag gttaaaataa
    20521 ccatacatac gaatataaaa atagctgaat attaaatgtg ctaatgaaag aaaagcaata
    20581 ctaagtgtaa aaaaaagatg tttcttctgc atgattgttc cagtccctta ctaagataga
    20641 tagtctttat tataccacgt gtgaggggag aaataaaaag ccgtttttta gtagatatga
    20701 aacaaaacaa aaacccgcat ttattcaacc tattcatcct tttaaaggat agattgaacg
    20761 tagatagaag tcgtttatcc agtctttttc attttgaaaa aaagctaaca gcatgctttt
    20821 tttatggtat actagcaagg aaacgaattc gcatcatgcg ataaaggagg aagagccccg
    20881 tggcagaaaa ggaaactttt tatatcacta cgccaatcta ttatccaagc ggcaaacttc
    20941 atatcggcaa ctcgtatact acgattgcat gtgacgcaat tgctcgttat aaacggttga
    21001 tgggctttga tgtgttttat ctcactggag tggatgaaca tggtcaaaaa atcgaaacaa
    21061 aagcagaaga actaggtgta aaaccgcaag aatatgtaga taaaatggcg gctgatgtca
    21121 agaaattatg gaaaacgctt gatatcagtt atgataaatt cattcgcacg acagatgatt
    21181 accataaagc agctgttcaa aaaatatttg atcgtttatt agaacaggga gatatttact
    21241 taggtgaata tgaaggctgg tactctgttt ctgacgaaga attctttaca gaaacacaac
    21301 ttgcagaagt gtacaaagat gacgaaggta acgtgatcgg tggtaaagcg ccaagtggac
    21361 acgaagtaga attggtaaaa gaagaatcat acttcttccg catgagcaaa tacgcagacc
    21421 gtctattgga atactatgaa gaacatcccg aattcatcca gccagaatct cgtaaaaacg
    21481 aaatgatcaa caactttatc aaaccaggat tagaggatct tgcagtttca cgaacaactt
    21541 ttacttgggg agtaccagtc aagagtgatc cgaaacatgt ggtgtatgta tggatcgatg
    21601 cattatccaa ttatattact gcactaggat atggctccga agatgaaagt ctattcgaaa
    21661 aatactggcc tgcagatgtt cagatggtcg gtaaagaaat cgttcgcttt catacgatct
    21721 attggccaat catgctgatg gcccttgatt tgcctctacc taaaaaagta tttgggcatg
    21781 gctggctatt gatgaaagac ggaaaaatgt ctaaatcaaa aggaaatgtc gtttatcctg
    21841 aaatgcttgt ggaacgttat ggtttagatg cactccgtta ttatttgctt cgtgctgttc
    21901 cgtttgggtc agatggtgtc tttacgccag aagattttgt ttcccgtgtc aattatgatc
    21961 tggcaaatga tctagggaac ttattgaatc gaacgattgc tatgatcaac aaatattgtg
    22021 atggactcgt tcctgactat gcctcacttg tcactccatt tgatagtgaa ctgtctacga
    22081 cggctgctaa cgttgtcgga cgttaccatg atgcaatgga aaaaatggaa ttcaatacag
    22141 cattggctga aatctgggtg ctgatttctc gtgcaaacaa atacattgat gaaacagaac
    22201 catgggtatt agcaaaagac gaagaaaaga aagctgaatt gaatagtgtc atgatccatt
    22261 tagctgaatc tttgcgtatc gcagcgatat tacttcagcc aatcatgaca gaaacgccag
    22321 agaagatctt caatcaacta ggattggact ctgaaacaat gaatcttgaa ggattacact
    22381 ttggcgaatt tccttcagga acaaaagtag ttgcaaaagg tacaccaatc ttcccacgtt
    22441 tagatatgga agaagaagtt gcttttattc aagaaaaaat gtctgaaggt actcaaacaa
    22501 acgaagatac agtcaaatgg gatccagaag aaacagaact tgtttcaacg aaagaaaaac
    22561 aaatcaaatt tgatgtattt gaaaaagtag aactaaaagt agcagaagtc atcaactgtc
    22621 aaaaagtaga aggggcagat aagttgctgc aattccgact agatgctgga gacagccaag
    22681 accgtcaaat cttgtcagga atcgctgaat tttatcctga tccaagcgaa ttgatcggga
    22741 aaaaagtcgt cattgtagca aatctgaaac cacgaaagat gcgtggacaa atcagtcaag
    22801 gaatgatcct ttctgcagaa gcaccagatg gctctttaca agtaatcgaa gcaccaaaat
    22861 ccatgccaaa tggctcagaa atcgcataga aattcaaagg gggtatgaca ctagttgtgt
    22921 cataccccct ttttccgaaa tttgagaagc aattttcgta aattccttct tattacttga
    22981 agccaatcat ataatacaga aacagagaac tatccaaaat aaatcctaga aaaagaagta
    23041 cgagcttcgc tgatttttgt taatttagaa taatactaat aggaatcgta aaaacagaaa
    23101 aaaagcataa tgataaaggg tcgggcagat tttaattgat tcatctggta ctctttttta
    23161 ttgtccttac attcataaag tttgaatgca atgactttat tacatctata tttatatcta
    23221 acagtagcgt tgatgtgtta attataagtt ctatcacttt atgttcaatt agtttgtgct
    23281 cttatatgtg tgattgaaaa tatagataaa aaaacaatca tcggctttga cttcgatgat
    23341 tgttttcttt attttgtaaa aatcctatca cttgatttta tggtaacctt ttatttttgt
    23401 cttcaaacgg cttggtaaga gagcagtaag cagtgcaaat gggatcgaaa agacatacca
    23461 gaaaatcgta cgtccgatac cataatgttg atagagcttt gtcaacatca cgtgtaacaa
    23521 gatcaaccct aaaagcaagg agccagctat aaaccaatga ttatcccaat gcatgacttc
    23581 actaagtgcc atcactccaa aaggatagaa ataaaaatag atcccgacta aggtcatccg
    23641 ccagttcttt gtaaatgttc cgatattcaa taaaaagaaa atcgctgaaa gcaaaatacc
    23701 gataggcgga aaaagcccaa ccaaaaagga atacaacatc ccccacaaga tcatcacagg
    23761 acctttgatc aatgcaacga ttgcgatgaa caaagtcagc aatattagtt tttgtgataa
    23821 cgttgcaaca gtcattaatc caaataaaat taatgtgaac caatgccaat gggactgctg
    23881 ttgttcttta cgggaaaaat cgacccggtt ggataaataa tctgctaatt tcggataaga
    23941 acgcttaaaa tccataaact gttcctcctt cgtggaaaag gtaggtttag tatacgaaaa
    24001 ataaaaaaga aatacatccg tcttaagagg gagtttttca ctgaaagtgg agaaaaaaga
    24061 atggtaaatt aaaatattct cagttatact gaaaattata ccaaatttca aagaagaaag
    24121 aggatgggaa tgaaaggcaa actaaaacga gaaatcaatc tgtttggcgc attagctacc
    24181 gttatgggaa ccgtgatcgg tgcaggcgtc ttcttcaaaa ctgctgctgt tactgctagt
    24241 actcagtcta caagtttgac attgctagct tggcttttgg gtggtttctt aacgatctgt
    24301 gctggattga cagtggctga attagctaca gcaatcccgg aaactggtgg agctgtaaaa
    24361 tatattgaag ctgcgtatgg aaaattgccc agctttttat taggatgggc gcaaagcttg
    24421 atttactttc ctgctaatat tgctgcatta tcgattatct ttgcgacaca attgacgaac
    24481 ttattacaat tatctaccga ttatcttttg ttgattgcag tgatcaccgc tgtttctgtg
    24541 acagggttga atttgttagg gacaaaagtc ggtgcttctg tacaatcagt gacgttgatt
    24601 gttaaattaa taccaattgc tgtgattgtc atatggggat tattgacccc aggacaagga
    24661 actgtccagt tatttccaat cgaagctgga aaagatgtta cttttgtcga aggattgagc
    24721 agtgcgttgt tagcaacgtt gtttgcttat gacggctggt taggtgtcgg tgcaatggca
    24781 ggggaaatga aacggccgga aaaagatttg cctaaagcaa ttattcttgg tttgagtttt
    24841 gtaacagtcg tttatctatt gatcaatttt gtttttttga aaactttgcc gattgatcat
    24901 cttgccggta atttgaatgc agcttctgaa gcatcagacg tcatttttgg aggaatcggt
    24961 ggaaagctgg tcactattgg gatcttgatc tcggtatacg gtgcattgaa cggttataca
    25021 ctaactggga tccgcgtgcc ttatgcgatg gctttagaag acgagctacc tttcagtaaa
    25081 cagctgacaa agctttcaaa gaagtttacg gttccttatg ttcctgcggt tttccagtta
    25141 gcagtagctt gtattatgat gagccttggt tctttcgact ttttaacaga catgttgatt
    25201 tttgtgatgt ggctattcag tttactgatt tttatcggtg tctttatttt aagaaaaaaa
    25261 gcgccggaat tgccacgtcc atataaagta ccactttatc caatcgttcc gattattgcg
    25321 attctcggtg ctattttcat tttaggtatg acgatgatga cgcaaacgaa attagcattg
    25381 atcggtatag gtgttacctt gataggtatc ccagtctatt atcaaaaaaa gaaaaaacgc
    25441 tcagaagagt aaaatatttc aacaacagac agaaaaagta cgaactaggt cctctagatc
    25501 ttcgtgcttt ttctatttca ataaaacaag ttcttctatt ttgatttttg ctacttctgc
    25561 aagtagtgct tatgtgctaa agtaagaatg aaaaggagag ataaacatga tatttgattc
    25621 tcatacccat ttaaatgctg aacagttcaa tgaagatatt ccagaaacga ttcagcgagc
    25681 acaagaaatg ggggtaacga aaatggcggt cgtgggcttc gatacgccaa cgattgaaaa
    25741 gtctttgcaa ttaagccatg actatccaaa tatatacagt attatcggct ggcatccgac
    25801 agaggctggc agttatacga aagaaatcga aaataaatta caagaacaat tgactttacc
    25861 aaaggttgta gcattagggg aaattggctt ggattatcat tggatggaag acccgaaaga
    25921 agtccaagat aaagtatttc gcagacagat tgctatcgca aaagaaatga acctaccaat
    25981 cagtattcat actagagaag cacttgaaga cacgtatcaa atcctaaaag aagaagatgt
    26041 tcgagatatc ggtggaatca tgcatagttt cagtggtgat tatgaatggg caaaacgatt
    26101 tcttgactta gggatgcata tttcatttag cggtgtcgtc actttcaaaa aagcattaga
    26161 cgtccaagag gctgcagcaa atgtaccaat cgaacgatta cttgtggaaa cagatgcccc
    26221 ttatctggca ccagttcctt ttagaggaaa gcgaaatgaa ccaggctata ctcgttatac
    26281 agtagaaaag attgctgaac ttcggaatct tccgacagaa gaagtggctg cccaaacatg
    26341 gacaaatgcg caccgattat ttggtctaag cgaacatgga taagatgaag atccaagaga
    26401 ttatagtcgt tgaaggaaaa gacgatacaa gaaggctgcg ggaagtagtt gatgcagata
    26461 cgatagaaac aatagggtct gcaatcaatg aagagatcct gatgcaaatt gaacatgccc
    26521 aagagactcg tggagtgatc gtttttaccg atcctgattt ttcaggagaa aaaataagaa
    26581 aaatcattat ggaagctgtt cctcaggcaa aacatgcttt tttgccgcgc acacaagcta
    26641 aaccaaagaa aaagggaagc cttggtgtcg agcatgcaag tgatgctgct attttagaag
    26701 cattgaaaaa ggtcgtgacg ccagacattt cccaaacggc tgtttctgaa atcactagac
    26761 aagacctttt agactatggt ttaatagctg gggcacaagc aaaagaaaaa cgagaaaaac
    26821 taggtgacga gttgcgcatc ggttacacga acggcaaaca attggaaaaa cgtttgaaga
    26881 tgtttcgtat tacgccgaaa gaactcgccc aagcaatgaa aatagtggag gaacaacatg
    26941 agtgaatatc gtgaaattgc gacaccctct agaacaaaag agattttatt gaaacatggt
    27001 ttttcattta aaaaaagttt aggacaaaat tttctgactg aaccaaatat tttaaggaaa
    27061 atcgtagaaa ctgcgcaaat agatgaacat acgaacgtga tcgaagttgg accaggaatc
    27121 ggtgcattga cggaacaatt agccaagcat gcaaaacaag tcgtagcatt tgagatcgat
    27181 gatcgtttga tccctgtatt ggctgatacg atgcaaccct atgacaatgt gactattgta
    27241 catcaagata ttttaaagac cgatttgtca acagctgtta gagaaacatt tcatgaggag
    27301 cttccgttaa aagtcgtagc gaatttacct tactatatta cgacaccgat catgatgcat
    27361 tttcttgaat cagatttggt tgtggatgaa ctagtcgtca tgatgcaaaa agaagtagct
    27421 gatcgtatct ctgctgaacc aggaacgaaa gcttatggtt cattatcgat tgctgttcaa
    27481 tattatatgg aagccagttt agcattcatc gtaccaaaaa cagtatttgt ccctcagcca
    27541 aatgtagatt cagcgatttt gaaactgaca agaagagata taccggcagt cgaagtgaca
    27601 gacgaaaaag catttttccg attgacaaaa gcggcatttc agcaaagaag aaaaacgctt
    27661 tggaataact tgcagcattc gtatggcaaa gatgaccaga caaaagcatg gttagcaaaa
    27721 agcttggaaa cagctggaat cgatccaaaa cgtcgtgggg agactttgtc attacaagaa
    27781 ttcgccgcat taagcaatgc gatgtcagaa aatatgcagt aaaaaaagag gttgtgacag
    27841 aagttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    27901 catgtttttc gtttgtttgg gcttgttacc gcagatgcaa gcttttgaac accgtttaag
    27961 aaagaggttg tgggcctgcc gtttaactct aagtcacaag gaacaatttc aaaaaacagc
    28021 ttctcatgtt tttgagttag ttggtcttat tacttgatgc aagcttttga ccactattat
    28081 gccgaacaag gagatgtgac acaaattttg tcacatctcc tatttttgtt tcttgatcaa
    28141 aaaattctta tacttgatac tcacactcat tttgtatgga tttagaaaag aatatgtgtc
    28201 ttgacagtta ttagtaagtg agttatacta agtcgacatt tatgtgtcaa gacatattag
    28261 gagagagaaa aaatgagaaa aaattcagtt cgaaaactaa cgatttctgc tttattgatt
    28321 gggatggggg tcattatccc aatggtcatg cctaaaatca tgattccacc agcttccttt
    28381 actttggcgt ctcatgtgcc attatttatt gcgatgtttt ttacaccagg agttgcagtg
    28441 gctgttgcgc taggtacgac atttggtttc tttttaacaa cgccggttat catcgcattg
    28501 cgagcattgt cacatcttgt gtttgcgttg attggggctt ggtacttgca aaagcatccg
    28561 agtatcgttt taaaaaatgg acagtttact tttgctaatt ggcgtttcca agggttcaat
    28621 tttgtgatcg gtgtgattca ttctttagca gaaatgctag tagtcagcat cttttacttt
    28681 attggtaata tgcctgaaac atattattct caaggatatt tttatacagt attcatcttg
    28741 atggggattg gtgggcttat ccacagttta gttgattata atattgctta ttttatagca
    28801 ggaacactca gcaaatattt tgatatcccc gtatttacgg cagcaaaaaa agaaaatcgg
    28861 aaagagtcga ttcaagggta cgcgaagaaa acgacatatt agacatgatc tctcaaaaaa
    28921 aaaactctca tctagtaatg gataataaat cctctaagat gagagttttt tgtcctaaaa
    28981 agagctgcac caacatcttc tggcacagct ctaacagggg gcaatttatg gaagtttgat
    29041 gacttgttct gtttctttat caaagaagtg gcctttgctg atgttgaagg caaggtcgac
    29101 tatttcgcct ggtttatgga aatctcttgc atctactttt gagatgaact cagtagaacc
    29161 agtttttgtg taaagcatcg tttcagctcc aagtaattcg gaaacgacaa cttctgattt
    29221 cactacggct tcaggtgaag cttcaagggc tacttgttca ctgtggatgt cttctggacg
    29281 aataccgaag ataacttctt ttccttcata acctttttca actaatactt tgttttttcc
    29341 ttcaggaata cgaagcttaa gcccatgtcc atctgtgata acaccatttt tcaatgtcac
    29401 attaaagaag ttcatggcag gagatccaat aaagccggca acgaacatat tgacgggtgt
    29461 atcataaact tctttcggtg taccgatttg ttggataaat ccgtctttca tgataacgat
    29521 tcggtcagcc atagtcattg cttctgtctg atcgtgagta acatagatgg tcgttgtttc
    29581 aagacgctgg tgaagtttgg caatctcagc acgcatggct acacgtaatt tagcatctaa
    29641 atttgataat ggctcatcca ttaagaagac ttttgcatcc cgaacgattg cccgtcccaa
    29701 tgccacacgt tggcgttgtc caccagataa agcagctggt tttcgtttca agtattctgt
    29761 cagacctaag atctctgctg cattgtccac acgttttttg atgtctgctt tatcatattt
    29821 acgtaatttc aagccaaaag ccatattatc aaaaacagtc atatgaggat acaatgcgta
    29881 gttttggaaa accatggcaa tatcacggtc tttaggtgcc acgtcattca tgacttttcc
    29941 gccgatattt agttctcctt cagagatatc ttccagtccc gcgatcattc gcaaagtagt
    30001 tgactttcca caaccggaag gaccaacaaa aacgataaat tcacggtctt tgattgatag
    30061 attaaaatca gttacagaat aattttctgc attgtcatat tttttatata cgtttttcag
    30121 agccatttct accataatac gttccacact ttctttttta gttttattaa ctatttaaag
    30181 tataaatgaa agcgttaact aacttcaatg tcatgttgaa caagaatttt ttcttttttt
    30241 tgtcaatgtg ctagttttaa gctttaataa atatccataa gaaataaaaa ctgatacact
    30301 ggagtaaata agtatgttaa caatatcaaa aggagtgtct cttatgaaaa tcgctttgat
    30361 cgctcatgac cgaaaaaaac ctatgatggt gaaattagca acagcgtata aaacaatatt
    30421 ggaaaaacat gaactttttg ctaccgggac gactgggcag aggatcacgg aagcaaccgg
    30481 cttgccgatc catcggttta aatccggacc gttaggcggg gatcagcaaa ttggagccat
    30541 gatttcggag gatcagttag atttggtgat ttttcttcga gacccgctgg ctgcacagcc
    30601 ccatgaacca gatgtaacag cacttatccg tttatctgat gtctatgaga ttccgttagc
    30661 tacgaatatc ggaacagctg aggtgctact aagaggactg gaagccggct tcctagattt
    30721 tcgaagaatc gtccatgaaa tagacaagca tccactttca ttttgatgga taagcgtatg
    30781 gaaaaataac gtttaatgtt cgcctatatg tagctataaa agctaaacaa actgataata
    30841 tgcgatataa tacgaacgta ttggataata taaacaataa tattagtttt tcgtatttgc
    30901 agtaaatgtg aaaatatgct atagtttctg tgggaaaaga tcccaaaaat aatacagagt
    30961 agaaaataaa gcgttaagtg ctaagaggat gggatgttgc ctcttggacg aaggacgtaa
    31021 tgttcgcggt tttattttcg cattcgctgc atgatacgat gtagcagctc ttaaaggaga
    31081 tgctaaaaat gaagaaacac cggttagacg cacgtatgat tgctattatg ggcttattga
    31141 ttgcattgat ggtcactttg tcccgactgg tagcaatcga gaccccattt attaaaatca
    31201 gtgtcacatt tattccacaa gtgatcatgg gcattctttt tggtcctttt tggtcaggga
    31261 taggcgcagt tttagctgat ttagtaggaa tggccctctt ctcgaaatct gcattcttta
    31321 ttggatttac attgaatgca tttattgaag gagcaatcta cggattcttt ttctatcgaa
    31381 aagagatcac ttggaaaaat gccattctgg ctacgcttag tgtcacgctg atcatcaact
    31441 tatttttgac accattatgg cttgcgttga tgtaccatgt gccattgttc agctgggtcg
    31501 tttgggcgcc ccgcctgtta aaaacagtaa tatggttacc gattcaatca attgccattt
    31561 attacgtagg acgtagtatt ccttacaaaa aaattttaag aagtttagcg atccacgcta
    31621 aataaaaaac aagcagtctt cccatcccag aagactgctt gtttttattg tgctgtgaat
    31681 tgtgcttgat agagccgtcg gtaataacca tctttttgaa gcagctcatc atgggtgccc
    31741 atttcgacaa tagctccctg atccatcact aatatctggt ccgcattttt gattgtggac
    31801 aggcgatggg caatcacaaa actggttttt ccttccatca ttgctaagaa ggcttgttgg
    31861 atttttttct ctgtcaatgt atcgactgag ctggtagctt catctaagat caacataggg
    31921 ggattactga tcatcgttcg agcaatcgtc aacagttggc gttgtccatc tgatatcttt
    31981 aacccttgac cgccaatctt agtatctaat ttttccggca aacgttctac aaattcatac
    32041 atataagagg cttttagggc atcattgatt tctgcgtcac ttgcttccgg attcccaaaa
    32101 cgaagatttt ctcgcaatgt actgtcaaat agccaagtat cttgtaacac cattccaaaa
    32161 cttttacgta atgtatcacg ttggatagag cggatgtcat ggtcatcgac tgtaattgtt
    32221 ccttttgttg tgtcataaaa acgcatcagc aaattaacaa gtgtggattt tccggcccct
    32281 gtttttccta ctatagcaat ggtttgacct ggataagcag taaaattgaa atcttcgatc
    32341 aaacgctgct tggggtcata agaaaaagaa acatgatcaa aagagacttt tcctttgata
    32401 tttttgggtt ccaaggcata ggcatctggc aaatcaggct tttcttctgg ctgatcgatc
    32461 aaatcaaatg ctcgttctag accagaaaaa gcggtttgca gttgtgtcgt gattccagaa
    32521 agttcgataa atggtttaga aaactgactt gagtagatcg taaagctgga aatcacacca
    32581 acagtgattg acgaaccact cttcaatgcc aataggccgc caactaaacc aatcgataaa
    32641 taagcaaggt gatctacaaa acgagaagac gggtttgtca aggaagaaga aaattgtgcc
    32701 cgttgtcctt tttcatatag atcagcattt agcagttcaa attgctgttg actagcttct
    32761 tcttgctgaa aagctttcac gattttctgg ttaccgattc gttcggtcac aaagcccgag
    32821 atatccccaa taatcctttg ctgtctatcg aaatttgttt gtgaagcacg agcaacgatc
    32881 caactcacaa ggaaaataat tggtgtagaa caaagaacga ccaatgtcag aagtggactt
    32941 agacgcatca taaaaataaa cgaaagaatg atgattgcca ctccagaaaa taattgattg
    33001 aaggccgcag aaattgcaat ggaaatattg tccatatcat ttgtaaatcg actgacgata
    33061 tttccgtggg agttttgatc ataataacga agtggcaatc gattcagatg agcaaatgca
    33121 tcttttctta attttgcaac ggataagtaa gctaaccgat tgcttaacac ttggatgagc
    33181 cattgactga ctacgttgat taaaacgata ccaccgaata gaataagtaa atggaacagt
    33241 cgattgaagc ggacctgtcc ttctccaatc atttgatcga tactttgacc aatttgtaac
    33301 gtcatcacaa ctgttgcaat cccattgatg attcctagta tcacagctag ccaaatgtct
    33361 tttgcatagg cagtaaggta aggaatgaat cggcggagcg tctgcaagga taattttttt
    33421 actttcataa ctattcttcc tcctgtgatg cgacgatttc ctgatattct gctgattgag
    33481 caagtaattc ttcatgggta ccctgagcag caatcttacc ttgatccatc actaagatat
    33541 gatgtgcttc ttgaatagag cggacacgtt gggagataat aatcactgta ctgccaagat
    33601 aagtctgtag cgccttacgc aaatccaagt ctgtttgata atccaacgca cttaaagagt
    33661 catctaaaat cagtaatgct ggcttggcaa tcaaagctct tgcaatcgtc agtcgctgtt
    33721 tttgtccgcc agagaaattt ttcccatttt ccatgacttt tgtatctaaa ccatttggaa
    33781 gctgacggac aaattcttct gcctgagcga ttttcagagc ttcccaacat tctttttctg
    33841 tggcgtcttt cttaccccat tgaaggtttt cacgaatagt cccggtaaat agtacagaag
    33901 tttggggaac aagagcaatt tgctgacgga gatgatccag ctgccaagaa cgaacatcca
    33961 ttccgtccat tataactgta ccggaactga catcatagaa acgcggaatc aattgtgtca
    34021 aagtcgtttt cccgcttccg gttggaccaa tgattccaag gactgtattt cgcttcagct
    34081 gaaatgtaat attttggaga gaaagaccag aatcaggagt ataacggaag tcaacatggt
    34141 caaaagcgat aattgcttca gaagaaaaat cgggagtagt actgacctca gcttcaagaa
    34201 tcgaattttc tgtgtctagc acctctttca cccgatttcc tgaagcttcg gcacgtgtga
    34261 agatcacgac gaggtttgaa acaacgatca gtgccaaaag catttgattc atataattga
    34321 tcaaagcaag aacttgtcct tgttgcaagc taccaatatt tactttgatc ccgccaactg
    34381 tcagcaaaca gatgatcccg acattcatga tcaaggttgt agcaggtgat aatagggcag
    34441 agatattgga aacacgtaaa taattgttcg ctagatcatt tgtgctttta tcgaacttct
    34501 ctgtttcttt ttttgtctgc gcaaatgcgc ggatgacacg aacaccgcta agattctgac
    34561 taacgtgttc attgagctga tctagttttt cttgcacttt ttggtagaga ggaacggttt
    34621 ttttgatgat aaaaaataaa atgattgaga aaataggcaa taatgctaag aaaaacagac
    34681 ctacttccca gtcgatcaca aaagccatga ccactgaccc gatacttaaa aacggtgctc
    34741 tgatcaataa acgaatgacc attgctaaag ctagctgaag ctgattgatg tcgtttgtca
    34801 ttcgggtgat caaggtatct gttccaaaat gattcaactc gttgtgagag aaagtattga
    34861 tttttttgat tagctgatta cgtaactctg taccaaatcc ttgtgaagct actgaagcat
    34921 agtattggca aatgatagca caaatcagac caatcacaga catagcgacc atccataagc
    34981 ccatttcaat gatttttcct cggtcattca tttttagtcc attatcgatc aaggaagcca
    35041 tcattaaagg caggacaagt tcgaagacag cttcaagaaa tttgaagaac gggcccaaaa
    35101 tgatttgctt acgatagtct tttgcataac gtaataactg aatcatatga ttcctcctat
    35161 tatttatttg tccacaattg aaaaaacaag tactaccaca ttgtagctta ggaaatttag
    35221 aatgaaaaga gaacaag
//
LOCUS       DACDTH010000026        34330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDTH010000026 DACDTH010000000
VERSION     DACDTH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.026"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            202..1077
                     /locus_tag="IWK54_001320"
     CDS             202..1077
                     /locus_tag="IWK54_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593449.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="IWK54_001321"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="IWK54_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="IWK54_001322"
     CDS             complement(1642..2973)
                     /locus_tag="IWK54_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9593450.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="IWK54_001323"
     CDS             complement(3122..3835)
                     /locus_tag="IWK54_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9593451.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="IWK54_001324"
     CDS             4010..5434
                     /locus_tag="IWK54_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9593452.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="IWK54_001325"
     CDS             5733..6440
                     /locus_tag="IWK54_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9593453.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="IWK54_001326"
     CDS             6427..6750
                     /locus_tag="IWK54_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9593454.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="IWK54_001327"
     CDS             complement(6839..7876)
                     /locus_tag="IWK54_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9593455.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="IWK54_001328"
     CDS             complement(8197..9180)
                     /locus_tag="IWK54_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9593456.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="IWK54_001329"
     CDS             complement(9595..10365)
                     /locus_tag="IWK54_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9593457.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="IWK54_001330"
     CDS             complement(10358..11293)
                     /locus_tag="IWK54_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9593458.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="IWK54_001331"
     CDS             complement(11609..13258)
                     /locus_tag="IWK54_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9593459.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="IWK54_001332"
     CDS             complement(13375..15978)
                     /locus_tag="IWK54_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9593460.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="IWK54_001333"
     CDS             complement(15990..17039)
                     /locus_tag="IWK54_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP9593461.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="IWK54_001334"
     CDS             complement(17065..17379)
                     /locus_tag="IWK54_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9593462.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="IWK54_001335"
     CDS             complement(17395..17841)
                     /locus_tag="IWK54_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9593463.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19818)
                     /locus_tag="IWK54_001336"
     CDS             complement(17842..19818)
                     /locus_tag="IWK54_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP9593464.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="IWK54_001337"
     CDS             complement(19976..20872)
                     /locus_tag="IWK54_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9593465.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="IWK54_001338"
     CDS             complement(20865..21545)
                     /locus_tag="IWK54_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9593466.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="IWK54_001339"
     CDS             complement(21546..22328)
                     /locus_tag="IWK54_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9593467.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="IWK54_001340"
     CDS             complement(22321..23238)
                     /locus_tag="IWK54_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9593468.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="IWK54_001341"
     CDS             complement(23402..23725)
                     /locus_tag="IWK54_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9593469.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="IWK54_001342"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="IWK54_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9593470.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="IWK54_001343"
     CDS             27853..28167
                     /locus_tag="IWK54_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9593471.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="IWK54_001344"
     CDS             28180..28554
                     /locus_tag="IWK54_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9593472.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="IWK54_001345"
     CDS             28564..28899
                     /locus_tag="IWK54_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9593473.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="IWK54_001346"
     CDS             28982..30322
                     /locus_tag="IWK54_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9593474.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="IWK54_001347"
     CDS             30400..31074
                     /locus_tag="IWK54_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9593475.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31307..31741
                     /gene="dcm"
                     /locus_tag="IWK54_001348"
     CDS             31307..31741
                     /gene="dcm"
                     /locus_tag="IWK54_001348"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9593476.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            31742..32449
                     /locus_tag="IWK54_001349"
     CDS             31742..32449
                     /locus_tag="IWK54_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9593477.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            32439..32729
                     /locus_tag="IWK54_001350"
     CDS             32439..32729
                     /locus_tag="IWK54_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593478.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32988..34172
                     /locus_tag="IWK54_001351"
     CDS             32988..34172
                     /locus_tag="IWK54_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9593479.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            34187..34306
                     /locus_tag="IWK54_001352"
     CDS             34187..34306
                     /locus_tag="IWK54_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9593480.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT        10701 a         6609 c         6254 g        10766 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    31261 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    31321 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    31381 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    31441 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    31501 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    31561 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    31621 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    31681 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    31741 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    31801 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    31861 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    31921 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    31981 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    32041 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    32101 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    32161 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    32221 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    32281 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    32341 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    32401 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    32461 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    32521 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    32581 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    32641 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    32701 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    32761 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    32821 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    32881 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    32941 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    33001 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    33061 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    33121 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    33181 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    33241 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    33301 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    33361 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    33421 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    33481 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    33541 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    33601 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    33661 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    33721 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    33781 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    33841 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    33901 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    33961 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    34021 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    34081 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    34141 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    34201 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    34261 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    34321 gacaaaattt
//
LOCUS       DACDTH010000027        32656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDTH010000027 DACDTH010000000
VERSION     DACDTH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.027"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            28..1626
                     /locus_tag="IWK54_001353"
     CDS             28..1626
                     /locus_tag="IWK54_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9593481.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1771..2511)
                     /gene="truA"
                     /locus_tag="IWK54_001354"
     CDS             complement(1771..2511)
                     /gene="truA"
                     /locus_tag="IWK54_001354"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9593482.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2702..3337)
                     /locus_tag="IWK54_001355"
     CDS             complement(2702..3337)
                     /locus_tag="IWK54_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9593483.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(3484..4725)
                     /gene="sstT"
                     /locus_tag="IWK54_001356"
     CDS             complement(3484..4725)
                     /gene="sstT"
                     /locus_tag="IWK54_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9593484.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4923..5921
                     /locus_tag="IWK54_001357"
     CDS             4923..5921
                     /locus_tag="IWK54_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593485.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5934..7952
                     /locus_tag="IWK54_001358"
     CDS             5934..7952
                     /locus_tag="IWK54_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP9593486.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8012..8728)
                     /locus_tag="IWK54_001359"
     CDS             complement(8012..8728)
                     /locus_tag="IWK54_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9593487.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8955..9497)
                     /locus_tag="IWK54_001360"
     CDS             complement(8955..9497)
                     /locus_tag="IWK54_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9593488.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9502..9729)
                     /locus_tag="IWK54_001361"
     CDS             complement(9502..9729)
                     /locus_tag="IWK54_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP9593489.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9808..11688)
                     /locus_tag="IWK54_001362"
     CDS             complement(9808..11688)
                     /locus_tag="IWK54_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9593490.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11877..12491)
                     /locus_tag="IWK54_001363"
     CDS             complement(11877..12491)
                     /locus_tag="IWK54_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9593491.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12633..13193
                     /locus_tag="IWK54_001364"
     CDS             12633..13193
                     /locus_tag="IWK54_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9593492.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13498..13812)
                     /locus_tag="IWK54_001365"
                     /pseudo
     CDS             complement(13498..13812)
                     /locus_tag="IWK54_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14066..15701)
                     /locus_tag="IWK54_001366"
                     /pseudo
     CDS             complement(14066..15701)
                     /locus_tag="IWK54_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16256..16819)
                     /locus_tag="IWK54_001367"
     CDS             complement(16256..16819)
                     /locus_tag="IWK54_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9593493.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(16834..17877)
                     /locus_tag="IWK54_001368"
     CDS             complement(16834..17877)
                     /locus_tag="IWK54_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593494.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(18010..18414)
                     /locus_tag="IWK54_001369"
     CDS             complement(18010..18414)
                     /locus_tag="IWK54_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9593495.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(18647..19282)
                     /locus_tag="IWK54_001370"
     CDS             complement(18647..19282)
                     /locus_tag="IWK54_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9593496.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(19548..20600)
                     /locus_tag="IWK54_001371"
     CDS             complement(19548..20600)
                     /locus_tag="IWK54_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9593497.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(20613..21551)
                     /locus_tag="IWK54_001372"
     CDS             complement(20613..21551)
                     /locus_tag="IWK54_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9593498.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(22002..22652)
                     /locus_tag="IWK54_001373"
     CDS             complement(22002..22652)
                     /locus_tag="IWK54_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593499.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(22752..23261)
                     /locus_tag="IWK54_001374"
     CDS             complement(22752..23261)
                     /locus_tag="IWK54_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP9593500.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(23462..24331)
                     /locus_tag="IWK54_001375"
     CDS             complement(23462..24331)
                     /locus_tag="IWK54_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9593501.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(24351..25472)
                     /locus_tag="IWK54_001376"
     CDS             complement(24351..25472)
                     /locus_tag="IWK54_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9593502.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(25474..25869)
                     /locus_tag="IWK54_001377"
     CDS             complement(25474..25869)
                     /locus_tag="IWK54_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9593503.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(25888..26685)
                     /locus_tag="IWK54_001378"
     CDS             complement(25888..26685)
                     /locus_tag="IWK54_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9593504.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(26687..27475)
                     /locus_tag="IWK54_001379"
     CDS             complement(26687..27475)
                     /locus_tag="IWK54_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9593505.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(27489..27965)
                     /locus_tag="IWK54_001380"
     CDS             complement(27489..27965)
                     /locus_tag="IWK54_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9593506.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(28037..30694)
                     /locus_tag="IWK54_001381"
     CDS             complement(28037..30694)
                     /locus_tag="IWK54_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9593507.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(30713..31117)
                     /locus_tag="IWK54_001382"
     CDS             complement(30713..31117)
                     /locus_tag="IWK54_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP9593508.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(31349..31606)
                     /locus_tag="IWK54_001383"
     CDS             complement(31349..31606)
                     /locus_tag="IWK54_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP9593509.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(31731..32063)
                     /locus_tag="IWK54_001384"
     CDS             complement(31731..32063)
                     /locus_tag="IWK54_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593510.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT        10265 a         6485 c         4952 g        10954 t
ORIGIN      
        1 aaaaaccaaa taatagatat catttttatg ttatttctaa gttttgctta cgtcatccct
       61 ctattcatgt ctaagggaat ctatcatagt gtcaatcagg atacttactt tcatttatct
      121 cgaatcatag gactagataa tgtatggagt tctccagtaa acttcaataa cttcgatcat
      181 catggaacaa tgatgaatat tttttatcca tggcttactc tttatccagc ttttttattt
      241 tataaaatga tgggaaatct tgttttaagt tataatatct actatttttt tatcactttt
      301 ttaacaatgg tcgtttctta tttttctatg aaacaaataa aaaacaatcg ttatatttcg
      361 ctgttatttt cgattattta tacattttcc gcctatcgtg ccattgacat attccatcgt
      421 gcttcattgg gagaagctgt cgctttaaca tttttgcctt tgatactaat ggggtgttac
      481 gaaatttata ttagagatta ccaaaaatgg tactggttgt ctattggcat gacgctagta
      541 gtgtatactc atttactttc tgtcgctatg gtttctgtat ttattggagg aacgttgttt
      601 ctttcttttt atttttggga ccaaaaaatc gctagactct taagtctttt aaaagctacc
      661 gtcctcacct tttttttaag tgcgggattc ttaattcctt ttattcaaca atctagagca
      721 caagaattga aagtcccact gggaaaagaa ttaagtggta tggctcctag tgatatgctg
      781 acacatattt taaataataa ctataacaac tatactattg gattattctt atttctcgga
      841 ttgattggtg cttttatttt tataaaaaaa ttaactgctg atgatttatt tattttcttt
      901 cttggtgtat ttgttctttt ttgttcaacc aatttatttc cttggcaact ttttaaccat
      961 acaccagtta agagtctaca atttgtctgg cggttaaatg gtttttcttc tttatttata
     1021 gcctatacga tgagtatagt gatctattat attttttcta ctaaaaataa ttcatggaaa
     1081 ataatggtct ttacgtttct tgcactaata ttgcatctct caggagtaag caattctatc
     1141 catgctaaca aagattcatt aacacttata gcacctgaag acgctgttgc tatagctgaa
     1201 aattacaatc atactgatta tgcaaacaaa gagtcaatct atcatcctga tatgattaat
     1261 aatgatcaat atttactagg taaccaggaa ataaatccaa caactcattt tatgagtaac
     1321 aaacttacaa ttgaattaga taattcgaat aataaaaata ctgtcttgac gacacctatt
     1381 tatcgttata aagggcaagt ggcttctatt aatggcaaac tagtacaaac aaaattatct
     1441 aagtttggca caactgaatt aactattcct ccaggtataa ataaagtcgt tattacttat
     1501 cagtacacaa aactagcaat tgcttcaaga tatttatcta tcgttacttt gattttattt
     1561 ttactttatc gatttacttt ttctaaacaa aaacaaccga gaagagaagt acaacattct
     1621 cactagattt ttatttaaga aagacttttt agctgtacac ataaacaggc taaaaagtct
     1681 ttttttatat ttctttaatt tgcttcatac attatttaac atcgatattt atactgtgaa
     1741 acatgcgttt catccattat caaagtattt ttatcgataa taaacttcat ccaaaaacag
     1801 accttgtgca gggacggttt ctcctgcatg ttgccgcact ttgctttcaa atatttcatc
     1861 gattgtttct atcggcatgg cacctgtgcc gatttctagt aatgtcccca tgataatccg
     1921 caccatttta tataagaatc cgtctccgac aaaagtaaaa tgaagcattt ctccctttcg
     1981 ttcgatagat aattcatcaa tcgtacgcac tgtggatttt tttgattttt tcaatgcaga
     2041 aaaaccaatg aaatcatgtg tacccactaa tttttcgcaa gctgcattca gcttgtccat
     2101 atccaactct tgagggaaat aaaagctgtg gtttcgctca aatgctgaag gaatcacgtt
     2161 gttccaaaca taatagctgt attttttgcc caccgcatta tatcttgaat ggaagcgctc
     2221 aggcatttct tcgattcttt tgatgacaat atcccgcgga aggtaacgat tcataaagtc
     2281 gatcatttct gccagtccaa tttctgtgtt tgttttaaaa tttgctactt gtcctcgcgc
     2341 atgggtgcca gcatctgttc ttcctgatcc aataatttct atctcttcat ttgtcatctg
     2401 atgcaatact ttttcgattt ttccttgaat ggttttatcg tcatctccaa gtctttgcca
     2461 tcctgaatat ctttttccgt catattcgat tgtcaacttg atgtttctca tattttagtg
     2521 gctcctctgt cttccatttg gtcttctctc taccttttta agattgtagc acaaaatata
     2581 gctccttgct tctctaagac agaagctata caagaaaact gtggaaatat atatttcaga
     2641 aaaacaaaaa acagccaccg gatgaagtat ccgctgactg ttcaatttga cggctcaagt
     2701 tttactgttt gagatgcaag atactcaacc tggcaatggg cacataatta aagaaaaaac
     2761 tgattctttg gcaaaaacct attttattca gtaatggcca agaatttata gaaggaattc
     2821 ttgccagtcc ataaatcgcc gcaaaaagca aactgatcac agacagtcca aggtataagt
     2881 ggcttacgtc catttgccca ttttttcgat agatcaagaa aagaagaact ggaaacaata
     2941 aaaaaccact gtaacctaaa cctgatccga tattatggac ccatgtcgag acattccacg
     3001 aagattcatg ggtattgata ctgaacaaac ctgtaaagat acaatcacct ataccataga
     3061 acgcgatggc tgctgtaact agggtagcta acacacgaga tgtactaaca aatgcagcat
     3121 acaatgcagg caatgacagg atgaaaaaga tccctgaaac cacagaccaa ataagaaacg
     3181 cttttcttac aggactggaa acctctccaa atacactgat cacgctcttc atttgatcta
     3241 attcaggata atacaatcct aatacgtacg gagtcaaaaa atcactcagc acgcctaata
     3301 ataaaaagaa aaaaccaaat ttacgtaaaa aatccatgat acgcctccaa taacaattac
     3361 ctatgctttt aattgttatt ataaacgact ttatcttgat ttaacagaaa cctctattta
     3421 cacgaacatc tatataactc attaaaaact cgaatcattt taaaaatact aaatatctaa
     3481 cgttcaacgt tcacttaatc catctaaaca atctttcaat ttgatttttt ccccacttct
     3541 tttacgcgcg cctagctctc ctattgcagt aaacaacaga tcacttgacg aatttaaggc
     3601 agtttccatt gaatcctgga taacaccgat aataaatcca ataccgacga tttgcatcgc
     3661 gacatcatta gagataccaa acaaactaca tgctaatgga atcaataaca gtgaaccgcc
     3721 cgcaacacct gaagctccac aagcagctaa agcagacaaa atacataaaa ggactttcaa
     3781 aacaaaaggt gcttcaatcc ctaaagaatg tacagcagtc aacgtcatca aggaaatggt
     3841 aatcgcggca ccgcccatat tgatcgtagc acctaacggg atcgatatgg aataagactc
     3901 ttttgtcaat ttcaatcttt cagccaacat catgtttatc gggatatttg cggcagaact
     3961 tcgggtaaag aaagcgggaa gtgcgctttc tttcaaacaa aagaaaatca atggataggg
     4021 attttcttta ataaacaaaa acgtgaggaa tgggtacaca actaatgcga caaacagcat
     4081 cgtaccgatc aaaacaagta aaagctgcag atactctgcc aatccagcga ttccagtcgt
     4141 agcaattgat tgataaacca atcctaaaat ccctatcgga gcaaatgcaa taatcacttg
     4201 tacgacttgt gacaacgcta tagatatctg atccaccaac tcttttgtcg attctgaacg
     4261 tgctcgaaaa gctagtccca acaatacagc ccagaataag acgccaaggt aattcgcatc
     4321 aataagagat tgaacaggat tttgtgtgac attcgtcaac atagtagata aaacactttc
     4381 caaactttgc ggtgcttttt cagatactgt ttcttgtaaa gtaataggaa cagtaaaaat
     4441 tgaacttaca ataactgcta caagtgcaga aagcaatgtt cccactaaat ataagatcaa
     4501 aataggcttc acgaatgtct tattcccaat tttatgcttg gcaattgacg ccatcgtcaa
     4561 gaaaaaaacc agaataggtg cgattgcctt caagcaactg acaaacaacg ttcccaatat
     4621 tgaaataaat gtccatgtgg ggaaaaagat accaaacaat attccgacta aaataccaac
     4681 aacaatacgt tgaactaacg acatatccaa aaacttctct aacaaatgac caccctttca
     4741 caattttaac aatcattgta tcattttatg caaagaaact aaaggtataa ttttctaaaa
     4801 attctaacaa tacaaacaac catttcttgc taattttgta ctttttctgt ctacacgttg
     4861 ttttttgaga tattctaagt taggatgtta ttaatgggca acttatcagg aaacgttttc
     4921 ttatgttttt gtccaatttt tttgtgaggt gtttattctt gaaaaaaata aagaaggaga
     4981 gttatggctt cctgacaatc tttgtcgctt gtataatgat ggttttacag cgtatattga
     5041 tttcagcagt cactattcct acatatgcaa caaaccctgg tcgattctac atctatacat
     5101 ttttacaaac ttccgttgtt attggtgcaa atttgatccc actttacatg ggttaccaat
     5161 atcaaaaaat cgcccgttta aaagttttac attatttaag tcgttttgcc tttatattcc
     5221 taatagccac actagtttgc aatatctttt tttacgtaca agctggcagc ttgaatattc
     5281 gtgattattg gcttatccta acacctatca gtcagaatta ctttacttat gctgtttctt
     5341 gtactttgct tttttgtagt attccttata cagttggatt tttaaataaa ttatcgaaaa
     5401 caacaattaa atatctacta ttatttttaa caattggatt agttggctgc tctactctat
     5461 ttaataaaga cccgtttgca ttacagaatg gacagagtat tttatggatt ttttatttat
     5521 ttttaatggg ttatggtttg aaagaatgga actggaaaga aaaggtccgc tataagcttc
     5581 ctcaagtatt tatcagcttg attgttttat tcggtttgat tattttgatg acacaaattt
     5641 cactcatcat cagaggaaat acagatacag cgctgaggtt ttctaaacca tattctctct
     5701 tcgcaatgta ttatactttc tcttcgtttt tatttttaga aatgctatct gataaattag
     5761 gtataaaagg aagaggaaat tttcttgctg tatttttgat tgtcactcaa gttatatgta
     5821 atttcccttt agtttcttat cgcgtatcta catttatgaa acgtgaattt ccaaactcag
     5881 ggaaggcatg gctgataaat atcgggcagt tttttatttg atatttatta gcagtgtccc
     5941 tcctagttat ctttttattt tatatacaaa aaatccgttt cattcatcgt ttcatcgaat
     6001 acttcgcttt tgattcgatt gaacaattaa ctcagcgttg gaaaaaaaga attcattggc
     6061 tttttgttca taaaagttat tttttagttg cagcattttt ttactgcttt accttcgtgc
     6121 agattttcgt tttagaacca aaagaaggtt ggaaagaaac tatccaggtt gccttaaaaa
     6181 tatttactca gcgacaagca cccatgatcc tgacaatcat cattattatg ggatttttct
     6241 tcttactatt gttgatttcc aatcgttttt ggtacgcctt gactgctacc ttgatcatta
     6301 acttactgct cactatttcg acagtaatca aaatggaaat gcgggaagag cctgtctttc
     6361 cttcagattt aaaaatgctg accggattgt cagaactatt atcgatggtt agcccagtct
     6421 tattaatcgt gggcggattg atccttctcc tattattgat tactagtatt attgtacaac
     6481 gtagactaca aaaacagtat tccttgaaga ttcattggaa acgtcgattt atcagtatcg
     6541 ctgtattatt aggatgtttt tcgggtgtct ttttcattaa tcacaaaaat tcgccttctt
     6601 tcttgctgtt taatttattt aaagtaaaca agactttctt taaccagcaa ggcgccgtaa
     6661 aagataacgg accagtcatt caattcctaa acaatatcga cattaaaatc atggaagaac
     6721 ctgctggcta ttcgaaagaa aaaattaatg gaataatgaa aaagtacaac acaaaggcag
     6781 atgaaatcaa ccaaacaaga agtgactggt taaacaatca gacgatcgtt ctcaatttaa
     6841 gtgaaagctt cagtgatcct gcacgtgtcc ctaatctgac agtacctaca gaccctattc
     6901 cttacatcaa aagcattata acagaaggaa caggaggatt gatgttatcc gtcggttatg
     6961 gtggcggaac agctaacatg gaatgggaag gactgacagg cttgagtatc agtaacctgt
     7021 ctgcttcgtt agttacacct tatacccagc tagtggagcg tcagaaaata agtccaaata
     7081 ttaccgatct attcgatgaa aaaatagcca tccatccttt tacagctgct ctttatcgca
     7141 gaaaacaagt gtttgataaa tttggttttg acaggttcta ttatttagac agtccggata
     7201 aattaactta tacggataaa atagaacaaa gcccacgcat atcggatgat tcagcataca
     7261 aagaaacctt gaaagcttta caaagcaata cggaaacgtc acaattcatc caattatcca
     7321 ctatgcaaaa ccatatgcct tatgatgatt actatagtga attgaactac actgctgaag
     7381 gaactgctgt cattcctaat agaagaaaag aattacaaac ttatatgcaa ggccttcact
     7441 ataccgatac agcagtaaaa aaattcatcc aagaaatcga caaaatcgat aaaccgatta
     7501 cctttgtttt ttatggtgac catttacccg caatctatag tgggaatgat tcgaaaaaat
     7561 atggattaga acagcaccag acagattatt tcatttataa caacaagtat agtcgtgaac
     7621 aagcagctac gttaaataaa gaagttgttg ctcctaataa ctttggtgct atggctcttg
     7681 agcatgcgaa tatcaaagta actccttact atgcgttgtt gacagaagta tctaatcatc
     7741 tacctgcatt aacaattgat cctacagaaa gcttgtctaa tcgttttaat ggaaaacagg
     7801 tctttgttac agaggatagt caaattatcc aagaaaaaaa tttaactgag aaacaaaaag
     7861 aaattctaga agattatcgt ctgatacaat atgatcttgt tgcaggtaac cagtacagtg
     7921 caacatgggc gacacaaaaa ataaaagact aacaaataaa ctgagcaagg ggttgtgata
     7981 aaagacatgt cacaacccct tgtttatctc attaatttgc ttgtccataa acgatttgat
     8041 taaagccatt tttcaattca ttattcgctg tctcgttgaa gtaaagataa ttatcgggat
     8101 tcccctgatt ttgttgtgtc accaatactt ttggctgatt ttgctgaaac ccgaatagat
     8161 agacgtgctt tttcagatat ggttctgtct ctgcatcgga atagacggct gtgatttgat
     8221 agtctgcttg acctgtccca ctttctgacc attctgcaga aacaggcact ccaccaatag
     8281 ccatcttcca ctccccgttc aaaatagctt gagggacttt aagaccatat aagtccacac
     8341 tcatttgatt cgtataactt ttgtattgtt gtcccattgt ttggccccat ttggtcataa
     8401 atgcctctaa ttgtgcggat ttgtctgcat tccacagtgt ttgttcaact tgttcgctgc
     8461 tttctgaaga ggaagatgaa gcctctacag aagaacttga agtagatgtt tgagttgttt
     8521 cagtcgtttt ggaagactct gacgaagtag aggtatctgc tgtttctgta cttgctgcag
     8581 ttgacttgct ctctgtgcta gttgttgtag tagttgcaac tgttgctttc gctgtttctt
     8641 ttgttgattc ttttttcgat tcattcccgt tggtacaacc agctaacaaa aaagctagac
     8701 caattgttac taaaatcctt tttttcaatt aaaaaactcc tttcgattac tcccacttct
     8761 ttattctatc aaataagcaa tgtgatttta ttgatttttc gtattttctc atcaatcttt
     8821 caaatcttct aaaaactgag aagcacccgc tgatgttgtc tgtaatacat cagcgggtgc
     8881 ttcttttatt ttaatcttcg tgattttcca atttacctca cgctttatat ttgtatttga
     8941 agaaacatag acttttagtc atcatcgtct tcctcatcca aatcttgtcg gacagttttt
     9001 ccttggagtc tttgtacttg gtagatgatt tctttattga aacttaaaag ttcaacaagc
     9061 aattgttgca agccatattt ttcttctttt tcagctaatt tgattgcccg tgtgataaag
     9121 acattttgga aatcaaaatc ttttgcaaag ttttccaaca ttgttgaggc tggtaaatat
     9181 ttgttttgac catgttcgta aatttttcca taacgcatga cttcttctaa agtagacgga
     9241 acgatttcat tttcaatcac taactcatga gagattttat tgaaccatct cgcttcttga
     9301 tcagcatatt ctgaaaaaag ggtacttaat tcttgcgaag atgtacccaa aacatacaat
     9361 tgtgcttgga tcaatttcat ttcttctaat ttaaaatttg ctaatacatg ccccaccatg
     9421 gcgccagcag ttggtttatg atggtcgata tctgcttgtt ttgtttcttg aacaaatttt
     9481 tcttccacta tattcattga tttacgcctc ttttactttt gctgattgga cttcataacc
     9541 taatttttcc acaactgcag ctaattgttc agggtggact tcatctggat caaagtttgc
     9601 tttgattttg ctcgcattaa acaaaacttt cgtattgctt accccaggct gttgagaaac
     9661 cgcatgttcg attttttgca tacagcttgg acaagctagt gtatctaatt gcataataat
     9721 ttttttcatc gtagttcttc cttccttttt cttgatagtc ttattctaga ttacagcttc
     9781 cataaaaaaa ttgattctta tcaagtttta cgatttttct tctttgcttt cgtgtccaaa
     9841 atacaataaa cgcatcgcgt taaagataac gatcaagata ctagcttcgt gaacgaacat
     9901 cccactagcc atgtaaataa acccggcaaa taagccgttg agtaaaaata agacgacacc
     9961 aatggcaatc actacatttt cacgggtatt taaaacagtt ttcttagcca agcggtaggc
    10021 atgaaccaac gattcaaaat tagattgcat caagacaacg tcactcgttt cgatagcaac
    10081 atccgttcct gatcccatag caatcccgat atcagccgca gccaaagagg gagaatcatt
    10141 gattccatct ccaataaaag caacgcgcaa tccttgttct tggtattttt tcacaaattg
    10201 aaccttttcg tctggtaata attcagcatg gacttcatca atgcctaaca tatcagctac
    10261 ataatcagca gtcatctgat tatcacctgt cagcatcact aactgttttg ctcctgcttg
    10321 tttcaactta gctaattgtt cagcgacttc tggtcgaatc acatctgaca cacctatgat
    10381 ttgagtgatt tttttgtcga ttgctacaat aatgaccgag ctgcctgttt gttgtaattg
    10441 atgtaaatct tgtacttgct ttggtgtgag agaaagtttg tgagcggcaa tcaatttggt
    10501 gttgcccgca caaactgtat gaccatcaat ctcagctgtg attccttgtc ccttgattgt
    10561 ctgattatct ttgactgcta attgttgaaa atcaatgtct ttctcctgag cataatggat
    10621 cacggcgcgc cccagaggat gatcggataa cgtttctaat cgagccacta aagctaagct
    10681 gtccccttca tcctctgtat agttcttgat agtggataca gcagtagttc ctttggtcaa
    10741 tgtaccagtt ttatcaaaga caaaagtatc gattttggaa aaagtatcca ttgcttcacc
    10801 gcctttgact aagatgcccc gtttcgctcc attaccgatt cctgctacat tcgaaacagg
    10861 cgcaccaatg actaaagcgc caggacaacc aagaaccaaa acagtgattg ctaaacgaaa
    10921 atcacgacta atcagaccaa caaccaacgc aataagtaaa acggttggag tataatactg
    10981 agaaaatcga tcaatgaatt tttccgcatg ggatttggta tcttgtgctt cttctactaa
    11041 ttcgataatt ttggcaaagg tcgtatcatc tccgacctta gttgcttcaa tcgtcacgta
    11101 gccatctgct aaaatcgttc cagagaagac ttcatctcca ctgattttga cgacttgtcg
    11161 tgattcacca gtcactgcgg cttcatccgc ataccctttc ccgtcaatga ctcgtccgtc
    11221 cacaggaata gctccaccag tttttactaa gacgatatcg ccttcttcca cgtcatcgat
    11281 ttccacttct tcagtcgttc catcttcttg gacaacacta gcagtagatg gggccatctc
    11341 ggtcaaagcc ttgattgact cccgcgtttt ggtcaatgtt ttttgttcca gatagctacc
    11401 aaacaaaaac aagaatgtaa cgatagctga ctccttgtat tctccgatca taaaggcacc
    11461 aagcacagca atagtaacga gtaaatcaat tgagataact ttgactttca ctgcctgata
    11521 cgcatggatc ataataggta tgaccgcaat aatagaagct atggctagag caatgttata
    11581 gaacaagaca aaaccagtaa attttccgat aaaaccaaaa ataatcaaca cagctgataa
    11641 ggcggtgata tgatttcgat gctcttggat ccacttttgt aatttcatct ttctccctct
    11701 tttcctttca ttcttacgag actaagtata gatacttatc catcataaaa aattgaccac
    11761 tatcaagttt agtcaaaaag gcggttttct tttggtagaa tacactacaa aaaatagtta
    11821 gcaagaagac tcactaaaag tgatcttgcc aactattgtc gtttctatcg aaattttcat
    11881 tgaatgattt tcctgtacag catacatcgg agatctattt ctttatctaa gatttttatc
    11941 ttgctaattt tttcctttag tctttcaaat ccacgtcgtt catagaattg atacgtacag
    12001 ccactatctg taaaaaggaa tatttctttt cctttctctc ttttttcaaa ttctgttagc
    12061 aacttggtac cgatgccttt tgttttcaaa tcaggatttg cagctaaaaa aatgatctct
    12121 ccatctggcg aatgcttctt taaatatgct gcaagcattt gttcatttgc tgtctcgtaa
    12181 gctcctttac tttctttagc aaatatattt tgtaaaaaat cgaatatctt aacatatatt
    12241 tgctgtccaa aagaacgatg tttctttttt tctcctttta tctccgccag caaaacacct
    12301 gccaactcgt cctctgcata caaagcaaga atttgagttg ctcgattcaa ctccatatac
    12361 aaaaaatatc ttccgtaaag attcagcaga agttcatttt ccatatatct ctcaaagttc
    12421 attccttcaa cagcaaaata gatagctttt ttgtaatctt tcttttctaa gggtttaaaa
    12481 gttagtttca tcatttcgcc tcctcaactt caatgattgt gttatactta cattataata
    12541 gacacagtgt ctactatcta taatcaatca tctataagtt tgtcgattaa gcgacagctt
    12601 tgctttgata tgtcggataa ggagataggt aaatgaaaaa gacagataga cgcgtcaaaa
    12661 aaacagaaaa agctttggct gaaacactat ccacattatt agtaaataaa aagattcaag
    12721 caatcactat acgcgaacta acagaaacgg cagatgtaca tcgttcgact ttttatacac
    12781 attataagga tatctatgat ctatatgatc aattagaaag caactttttt cgggatctaa
    12841 atgaaattct ctcttatgat ccagcccata gctatgagga actatacact cgcttgattg
    12901 actatgttta cgcaaatact tttttatatc gcttatttat cctcaaccat ggtgatattt
    12961 cgtttcagaa gaaagttaaa caagttattg aagaaaacta tctgaacata tggctatatg
    13021 aagacaagaa aacgattatc acagaagaaa tgagattctt tactacgtac cacgtccaag
    13081 gttgtctatc cgttctcaat cggtgggtag aaagcaatta caagtactcc aaaaaagaat
    13141 tattagaact cttacagaaa ctgaatgatc acatagagaa aatcatgcct tgaataaaaa
    13201 catactaagc ttagttaaga agaaccgtaa ccttctcgta taacaactga atcttattta
    13261 gccaaattgt ttactgaaga ttttttgagt aaaatttcta taattcctac ttattgaaaa
    13321 gtagtttgta gtgctgcttc tccctctcta agtgatatat cttgttgtac caatcaaatt
    13381 cacctcatca ttaataaatt acttttaggc taatttatta caacttatag gttgaagctg
    13441 taaccaattt atagtcatat tatattttta tcatccagtt tattgaattc ttctcgttta
    13501 cctcaattca ttttcaaggg gaatcagctc atcatctaac attttacgcc atgctcgctt
    13561 catttcttgt gggtatttaa atggtttcgc ttctggatgt ttggctttat atccttcgta
    13621 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
    13681 cagattagaa gcatgtaatt ttttctcaga aacaaaccac tcattggaaa agacatgaat
    13741 caaatcatca cttacttgtg taaaatagcg acgtttaatg acaaagatat cttcattgct
    13801 atctagtgta ccttcatttc tttgtgctta aattcaaaga taattttctc aataaagatt
    13861 tttgattcca gtaagattcc tttactcctt ttacaacata gcgttgtcct ttgttttgaa
    13921 acatcttctt cccctctttg ccattaaaaa tagcccaatt ctctataaag aagattaagc
    13981 ttcattatta taatataaag tttaaatttg gtttattact tactttttct tattctctag
    14041 taagctaata ttgaaaaaaa tgtaatcaga tcattatttt tatatgttgt aacttcctga
    14101 taagaaaact aattaaaaga aagaggtaac tacataaact aattaaaaaa gctaacttca
    14161 tagttggagg ataaatggaa gaaacttcaa tgattgtttc tccttgtgtt ccttcaatcg
    14221 acactagtcc tattatcaat ctctcttttt tgtccattta cttttgcgac tgttccccaa
    14281 taatcaaata caggaagcgt tattttttgt cctttagatt catgtactgt atagactatt
    14341 ttcgaatcca tcacttgata aggcatagta tgccattcct tgccaattct tacttcatga
    14401 ttctgcaaat gttctttgta ttgtaatgct tccgtgggtg catagtcata aagtcctccg
    14461 ggaaggagtg tcgtgtaacg aataagattt tcttgattca ttaccaagtg atctctctga
    14521 gtgacaactt tatgttcata gtttgctttt gttgcagcag atacagccaa cagtaataaa
    14581 acaattgccg ttatattaac tacttttctt tgcttagctg atgtgctctt catgaaaacg
    14641 aacgataaaa tcaacgagcc aaacagtact tgaaagccta agattcgcca aggaaattga
    14701 atcatttgaa tgggtgtatt ttgtaataaa gaccatggaa aaaatttggt ggataagagc
    14761 caagagacta ccgcactaag gtaaatcact ttttctttgc gattaaatcc tttaaaaaag
    14821 aagagtggac agatcaaaga aataatacca atgattccta ccgtaaaggt tgtcaggtca
    14881 ttattcatcg caccaatcaa actatccgag acatttaatg cttggtgttg taaggtcgtt
    14941 ttatctggct cattaatcga ttgaaccatg atttgtcgga acatcggata ccaaaagtat
    15001 gctgttaaaa gaatcgtcat tcctactgcc tttgacaact ccacaatcaa ccggatgctt
    15061 tctttttttc ttgtttcact tactacaaag ccgcctaaga ctagtaagcc aacggctatt
    15121 acactcataa aagtggaaag aacatgtgta taaatgagta agctcatact gactgctaat
    15181 ggaattgcac atggcttttt ctcataaatc agtgcgtaag cataatacat aactatcggt
    15241 ataaccgtaa gtgcaatcgc ttcagcaata gcagaacgat gataaatatc aatcgtacgg
    15301 tacccataaa atgtatataa acagctaaat ataaaagccg catcgattct ttttaaaaat
    15361 tttagtccat aatgaaacat cagcatgata gtacaaacat tcaatagcca aacatataag
    15421 atataactaa cgattaaatt attacttatc caataaaaaa tgactgccgg aaaaaaggtc
    15481 aagtagggat aaaaataatt cactccgctc ccataatgat taaacgtcgt aaaattgatt
    15541 ggaccagaaa aaattgaaga taaccctttg actctagcaa aatggaaggc aatatccccc
    15601 gatgtagaaa tagtatgaac atttctaaaa taattgattg aataaagtcc agcaataatt
    15661 gctaggagaa tggacaatat tatttttttc tttacttgca ttacttaatt atatactcct
    15721 ttcatggatt gctttatcta gtataactta aattttaaat aaatcgttat ttatttttac
    15781 tctcacttaa aaaagcacta aaattataag agagggaggt aatggtctga acaccaagaa
    15841 ttgttccaac atcgaattgc ttaactaatg agttcaaagc ttaattatgt ggctcttata
    15901 tttaccgagc gtggattgcg atctctttag cgaaacaaac atctttcata aaaaaaacag
    15961 ccatatccgc tttcttggat atgactattt actactactt tactatcttt cattcttctt
    16021 gtctcactta tgataaggcg ctgcgtatca tgagtgaatg atattaggaa aacttcataa
    16081 aataaatctt ttatacattt ccacttctag atggtcattc atgttagaag aatcaaacta
    16141 tttttttact gtcgtaataa tattaaaaca ctagaacaaa tcaattaaat tccataaaat
    16201 ataaataata gacctagcat catctggtat aaaatggatg ctaggtccgt tatatttact
    16261 caaaattgat tctacgaata gatcttctta tcatttctat tttagtagtc atgcgttgtt
    16321 gttcttctaa aggtggtaat ggtataagta gtttagataa atttgtacca tttacatttg
    16381 gttgcccagt tccgtaagaa attttttcta ataacttcca atataatggc gagtctaaga
    16441 agcagtctac gtattctgat aataatttct caactaacct aattcgaatc aagtaagaag
    16501 caaaaacaga ttcctcactt atttccttaa ccaagaaact ttttcccata gttcctcctg
    16561 tccttgctat caaaatgtca ttttcttcca atcttaaatc aactagttta gaattactaa
    16621 tatcacagta aggcacactg gaccaattta cacgtccctc ttgtatatct gtgattctca
    16681 aaaacttaac attgccttgt tttttcgccg aatctgtata gccatattga atcttagtag
    16741 aaatacttcc taaataaacc catagtcgaa catcgctaga tgatttttct tgtcgttctt
    16801 tatgaacaac agtgatcatc ttgagttgtc cgactatttt ttagttgcag caatcttttt
    16861 gggacgctta tttgcatgaa gcaacgttgc taagtctagt aataaaacat tggctaacac
    16921 aggattggct ttctcgcgat acgctgcggc gccactttca aacaatactg ctagacgatc
    16981 ttctacaaca acgatttgtt ccaaatacgg tggaaatttg attcgtttta ggatccggct
    17041 aggagagtcc cgctcggaca ctaagagttc cgaggtataa ggaccataag aacgactggc
    17101 taagagataa tggccataaa aagccacacc ttgaatctta tcagatgttt ttctgctcat
    17161 tgtcacggtt ggttggattt tgtctttgcg gcgaattgct gttttcattt cagctatcgt
    17221 cggcaataga tagaaagtca actgtccttc accttttagt gtgaagttgc cagataccag
    17281 ttgatcattg ttttgagtca aatacgatgc ttgtggaatc tctgagagat cagttgtcag
    17341 ccaataagcg attggctgtc ccgtctcttc agatgtatct gctaagatat cgatcaaacg
    17401 taaagcggct actcgtccag tagcagatcc tgtgattgtc aaccataaaa gttcccgttt
    17461 tgtatcatat actaagcccc ctacatgtgg ccgtttaggt aaaatcagca gtttgatcgg
    17521 ctgtcctgtt tctctatcca gtacatgaat aatagaatga tgctgatggt catagcagta
    17581 ggcagaaata aacacaaaat cttgagaaaa agccaaccct tgcggcgtca tccattgaca
    17641 aacctcattt tcgccggttt ccggcaccac cattcttgtt tctgttaatc caggaatgtt
    17701 atattgtttt gcttcagcct tttcccaagc tgcttgaaca ataggatacc gttttaactt
    17761 tttttcaata tcctgatgta tccgttgctg cttccaatgc ttaaacttta ggacatagta
    17821 aacaagaagg ccaatgacca gcaacactaa aatcattatt gctacccgct gtgtcatttt
    17881 tttgcctcct tgttctattc gatcaatcgt ccatataccg ctattaatta ccgtaactac
    17941 atgtttcttt tactagcgtt tctttatagg atctttcata atctcattta tagtaacatt
    18001 aagaatccct tattctacta tagtcacttc cacatcaata ttgcctcttg ttgctttaga
    18061 gtatgggcaa aatgcgtggg cttcttctgc gattttctct gcttggtcct tctcaagtcc
    18121 tggcaaagaa acttctaaaa cgaccccgat ttggtagccc ccatcttcta attgaaataa
    18181 agatacccgt gctttgactt cagaatcaga ctcaagatta ttttcttcca gcatgtgttg
    18241 aaccgcgccg ttgaagcaag atgcgtatcc agccgcaaaa agttgttccg gatttgtccc
    18301 ttcactatgg attcctgggc ggtcgatctt catgtgcata cttccgtttg gtgcctcaac
    18361 ttcgccatca cggcctccac gattgatcat tgctgtttcg tacatttttt tcattgagat
    18421 catcctttct ttcgtcgttc tatattaagt gtaacgagta ttttttaatc tgacaaaggt
    18481 aatgcttgca ttcggcgaca tcagttagac aacatcgatg atcagtgata gaagcagttt
    18541 atctatacaa aaaaagaagg gttctatgat atttggtgtc cttactcgcc ttgagtaaca
    18601 acaccaaata tcatagaacc acttaatcag cttcacactg ctttttctac tttcgtagtt
    18661 gaaaaaaact taaacaaatc atcggcaaat agttaaagaa aaaacttaat cgttgaaaga
    18721 agcccaattg cttaaagaag gcaaactgac taatcgacgg aatccgtgct aatccgtaaa
    18781 gacctgcaat tagtaaacta ataactgtta aaacaagata aaaatggcta agcgtacctt
    18841 gaccactttg acgataaagc agaacaagta acaatgggaa aagtagaaag cctgcatagc
    18901 ctagcccaga acctatatta tggatccaag tcgatagatt acaagagctt tcattcgtat
    18961 tgatactgaa taagccagta aaaatacaat cacctatccc atataaacca actgttgcaa
    19021 caacaagtat tgcaagtgtt tttgatgttc caacaaatag gtgatacaac gcaggcaacg
    19081 aaagaacaaa aaaaagtcca gatacaactg accagaccaa gaaagcacgt ctgactggac
    19141 tatctacgtc accaaacaca ctaattacgg tagtcatttg attcagtttg ggataaaaaa
    19201 ggccaaggat atacggtgtc gataaatcac tcatcacacc taataataaa aaataaaaac
    19261 catatttttg caaaaaaatc attttcttcc cctacttttt agtgattatt cgatacatcc
    19321 aacaatcaaa ttttttctca ttttaacaca tatttttatt ctagttgtct tttgaacaat
    19381 caataacata aaggtgagtt tcttctacat tttaaaaaat cgaatggcac tgaagagttc
    19441 agttgtcagc atcaatctcc tactcaaaaa ggcaaaaaaa cgtaagtcag atgttgttga
    19501 catctcactt acgcatccat tgattgaaaa tttcttagta aaatctacta gcctaaaaat
    19561 ttgaagactt cttctcgatc atacagttta aatactttct cgcataaaaa ttgagtaatt
    19621 agtgccgcaa aaacagggat gactaaccaa caaagaacaa caagaaagcc atttagccct
    19681 gcatctaaag aagccaacgg accaacaagt ccgaccacgc cgaaaccagc agaagcaggc
    19741 gtacctgaaa tagaaaatag agcgacagga atagctgtaa tcactgcact aactaaacaa
    19801 ggaataagaa tcacaggttt tctaaataga ttaggcatca tcatcttcat agcgcctaaa
    19861 gcaatcgcaa ttgtcacacc tgacttatta accttccaag agttcactac taaaacaaaa
    19921 gtggttgcag ctacgcccat cgcagccgca ccagcagaaa caccatctag ttggattgcc
    19981 attccgattg ctactgttga aataggagag ataatcaaaa aggcaaagga acaaccaatc
    20041 aagatagaca ttaacagcgg ttgtaaggta gtgaagttat taatcaatga tccaattgca
    20101 gaagtaattg ctgttacaaa agggaataaa taaaaaccaa gtaaacctgc acctacacct
    20161 acaacaatcg gtgttaacac aatcgacact gacccaaaac tatttccaat cagtaaaatc
    20221 agcccgacag caatcgatgc tgtcagcatc gtattaataa tatcgcctgt accagcacca
    20281 acgtaaacac ccgtttcacc caaatcaggt gtgaatttga ctacaccaga accaacaaaa
    20341 gaagctccgg ctacaaccat cattctcatc ggatcaaatc caaattgtaa ggcaatcaaa
    20401 ccaccaatga tcaaaggtgt tgctagttga aaaataactg ctaactgtgc gatcgttacc
    20461 gcaaagggat acgcactaaa atattttaaa atggcaccta atactgcatt aggaatcaat
    20521 ccaataatga ttcctgttgc tgtaccagcg aggactttat tcagaaaaat tttcggtgat
    20581 attttttgtg aagtttccat tcaatctctc ctttatttta cgttcaaact atcttctttt
    20641 gcatggacaa gctcagtcaa gaaatcacaa taaggcgtcg caacaccata tttcttccct
    20701 tttctagaaa ttgcgccatt gatataatcg atttctgtta atcgatggtt cttaatcaag
    20761 tcttgataca ttgaaggata atgtaaccca attgtttccg ggtcaaaaca agattcacaa
    20821 tgtgcaatga cttctgggac atctagttca atcttctcta ctgctgctac tttggcaaac
    20881 tcattgacaa tcgtcgccac cattttatga gcagtcgatg tttttcctag ctcagccata
    20941 ttaacgtcta agattgtgca tagtccgttc attgttccgt tcacacatgc tttgcgataa
    21001 atagaatagt gaatattgtc agaaaaatga gcatttaatc ctgattcaga caatttgtct
    21061 gctaattttt ttgcagctgc ttcttttcca tcacctaaat tttgtaattc tactgaacca
    21121 cttccaaata atttgacctg accaggacct tcaagacctg ccgtccacat ggtatttcca
    21181 atatagatat tttccattgg tacaaatttc tcaataatat cttcatgccc gataccattt
    21241 aatagacata agacctctgt atcttttttg attaatgatt ggatatcctg cagcattttt
    21301 tccagctgca tcgctttggt aaatagaata atcagatcaa cttgatcttc tttttctact
    21361 tcggattgaa gaacgattgg tagttttgct tctacctctt taccattaaa atttgcttgt
    21421 aatccatgtt ctttgatttg ttggacatgt tctgcccagc catcgattaa taatacttcg
    21481 ttcccacttt ggtgaagcat caacccaaag cgacttccca ttgcgccagc gcctgcaatt
    21541 gctattttca ttgattcttc ctcctttttc tttctattat actcatttga gaatcttttc
    21601 acaacctcgt tttgttattc ttctcttatt tatcatgttt atatccacca ttcaagttat
    21661 tttatcagca aatcaataaa aactggaata gaatcccaat ttcatcgtaa gagaaactag
    21721 actagcaata agaaaataga aacattcata gaaaaaatct cactcctctg attataaaag
    21781 agggggcatt aacctcgtgt atactaaaac cttccattca ttcgatggat gattttcttt
    21841 ttgtgaaaaa atattcattg tagtacgatg ttgcgcaaca cagacggatg gtattgctta
    21901 ttatttatta aaatcgaaca atacacttat ttaaaaactt ctttcccaga ttttcttcca
    21961 tttttacagt tataactagt attatcatga aaaatagcct tttattgtct atgggaataa
    22021 taatttaaaa ttggtacttt tttattaatt agttcatcgc ccaataatct ctccagtttc
    22081 ttagcatctc ctgactcaac cggaattatc caagcccagt ctaaataatt ttttaaaatc
    22141 tcatcatatt ttgcaaatat ttcattgcca tattccgtcc ttaaaattat attttcagtg
    22201 ccagacaagt gatttatatt gagttgataa aaaagatctc catctaaaaa aatatcatta
    22261 gaagaagatt tccccacagt aatgaccatt ccatcagctc tatgtgttac atacaaataa
    22321 ttgtatgacc tcatttcacc taatgaatca attatttctt gccctttagc tctaatatat
    22381 tgatctctat tacctatctc actcttattt aacaaaagtt ttttcatgtc aaatttaatc
    22441 ggctgtttta tttttgtaaa ttcgcttcta ctactaatcc aatccgagat taatttttca
    22501 acatgtccca gagtaaactc tgatttattc gctaaatact ttttttgttg tattaaagac
    22561 atctttcccc gaagctcaaa attgagccta catcgattct ctatctcttc aaaaagcatc
    22621 ttatcttcat cattcttata ttcatcattc ataaaaccaa tccagctcct cgattaagat
    22681 aatattttaa tctcatacaa aaactagctc tcatcaaaac aaccattgtt ttgatttctc
    22741 atgaaatttc tttatacttt tctccgatta taaattccta accccttgag aaaattacgc
    22801 atatatttat ctccgcaagg cttaaaattt cgttggcctt cttttctcaa aaatgccccg
    22861 atttcgcctt ttgatggata gatttctgcc tcagcaaaaa cgtctaaaat atcatcagtg
    22921 gtcatagaca aggcgatttt taattttttt agcaacacat tgttgatata tttgatttcc
    22981 ttgctttttt gcggcgtgtc tactatcggc ttatcttttt gcggaccacg cgccaagaga
    23041 atcaggccat ttaggaatgc gtcaaaaact tggttgttga tggttttttc atacacatta
    23101 ttttctgcat tatctttgtt cttatcctgt actttggtca gtaccgcctg agcttcttct
    23161 ttagtcagtg gtacaccgcc tagctcaaaa gctttaatca aatccgtatc tttcaaatca
    23221 atcgcataac gcaatcgcaa taacctatca ttgttattca tttgttcctc cgaagtattt
    23281 agtctttact ttcttacgta ctttttgtct tgctaagatt tttaggatca atgataaaaa
    23341 ccgcatcact tatgataagg cgctgggtat catgagtgaa tgatattgct gaatacaaat
    23401 agtccgacca cattaagttg atcggacttc ataagcattt tattgactaa tacttttttg
    23461 cttaagaatt gattctaaca tcttttgcta aagttaagca tcctatagtt cctgcattat
    23521 ctgctaactt aggtgtgaca atgtattctt cgatatttgg cgtatcgaca tagccgttaa
    23581 ttagttctaa aaaacgtctt tgtactttct ttttcagatg ttcttgttgc atgacaccac
    23641 caccaaaaat tataatatct ggtgaaagca tcagggtcgt attacaggca cattgtgcaa
    23701 tataatccgc ctcgatttcc cagaacggat cttctataga aagatcttgt cccttggttc
    23761 ccgtacgcat ctcgatagcg ggtccagatg ccaacccttc gagacaatca tgatgaaaag
    23821 gacaagtacc agagaaagta tctcctgaat gtctcttaac taacgtatgc cccatttctg
    23881 gatggctaaa cccttcaaca aaaatcccat cttgtatagc tcctccacca attccagttc
    23941 caacagtgta ataaacaaca ctggataaac cttttccatg acctgaaaca tactccccgt
    24001 aacaagcagc attcacatcg gttgtccagg caattggaat ttcgaaatgc ttattcaacg
    24061 ttccaacaaa atcaaaattt ttccaggcag tttttggagt agaggtgata tgtccatagg
    24121 tctttgattc acgatggata tcaatcggtc caaatgaccc tactccaatg ctttctaatt
    24181 gttctttata gtgattaaag aaatcaatta ccaaactcat tgtttcattc ggtgtagttg
    24241 taggaaaact cactctttct aaaaccttta aatcatcatt gccaatggca caaacaaatt
    24301 tagtcccacc tgcttctatt gagccataca tttcttactc caccgtcctt ttatctagga
    24361 attcgttctt ccctgtctaa tagtttttcg aaaaactcat gtggttcctt ttggtaccaa
    24421 taggcaacag aagaaatatc gtcttgacgc tcataaagct tgatgtcatc atttcctagg
    24481 gtttggaaag taacacgtaa attttctttg aaactaatgg ggtcgggtaa atgccagcgg
    24541 tacaacccat gcatcggtaa agcatcatcg ccaaaaccat gaactggatt tggattaggt
    24601 tttccagcac taaaataatc ccgggtgtga tctcttttag attgaaaagg atagcccata
    24661 aatagcgtat taaaattttg agcatatgcc ctttctccaa agttcttttt atggaaagcc
    24721 caagcgccgc caaaataatc ttctgctcct gtagatgtta ttgtagggaa atcttcatct
    24781 ccatctatat aaaacttaaa ttctccttcc ccccaccagt atctttccaa tgcgcacaaa
    24841 gcaaagtagg ttcctacata atacccagtg cctttgattc catcaataag ggtgtaatct
    24901 tttccttttg tcgtgatggg ttcacggttc catttagcat gaaaatacat cgcatcatcc
    24961 ggtacttcag tcaatgcata attaaatgta taaaaaatgt gcttaataaa ctcaggatgt
    25021 tcattcgtta aagtaatctt agcttttttt cgaaatggca tctcaaaata gcaattcatc
    25081 cctgatgttg gattcactac gattggtaag gaattcactt cgcatcttgt cccaaaccca
    25141 ttacaaaaaa aatctcctaa tggactttct acagaaggag tggattcatc atcccaataa
    25201 atacgcaaaa ttaaatcacg taagacgaaa ctcccagcga ttgttttgtc tgtcactgtc
    25261 atccaaaaat gacgtaaaat tccagggcct tccatttctg ctaaagtaat tgtctctcca
    25321 gcatttatcg gaacagaagg tgaccctttt ctcccaattc ctagatgact cgctgtcatt
    25381 gccgctttcc ctttttcacc atttttattt tctggtgaaa tagttctact ttcctctccc
    25441 ttgtggagcc atatattatt taaacaattc attttattcg tcctctatcc tttcttcact
    25501 tttttgatta ttcatataga tgatttgttg ttgtgcctcc gcaataattg ctccaatatc
    25561 taaattatca tttcctaact gcaaagcgat tgacattgcc aagggaagat tcattcctgc
    25621 aattacgtgc actctgtcag aaatataggt aaaaaacttt tgatttacac ttcctccagc
    25681 catgtcagtt aaaacaatta tttcgtctgt agtatccact aactctttaa tttgttcatc
    25741 tatatcatca atttcttcga catatgcaca taagctacta atttgattat gcatacctgt
    25801 taaaaactct agtgtctctt taaaaccctc agctagtatt gaatggctag caatgatcaa
    25861 tcttctcata attcaaatcc ttttccttta cgctaatatt ccaaatgcag ccataaccat
    25921 cgcaaaaaca ataacgagta gaatcaaaat ggtcatactg atttttttct tactgagtag
    25981 ataatatgct gcagcagtaa taattactgg aattaatgtt ggtaaaattt tgtccaacat
    26041 tggttggatt ttcatactaa cttcaccaaa agtgaattta agtggcgttg aaacactgat
    26101 aactgaagca attaatgatc caactactgt tagccccaat actgaagcag cttcagttaa
    26161 ataattaatc ttttcaccgt attcagtaac aatcttcatt ccctgcttat aaccaaattc
    26221 aaagcctctc attcggaaaa agaagattac catgattaaa atgaaccata aaaagattcc
    26281 tacaggatta ttttctaaag ccatataagc cgcaattgat cccatgatgg ttgggatcat
    26341 cgtcatcaaa agagagtccc caataccagc aattggaccc atcgtaccaa ctttaaagtc
    26401 attaatcgca tcttttgctt ctaatccttg cttttcctcc attgcaacac aagcacctaa
    26461 aataatggct gccagccaag gctgtgtatt gtaatactga aagtgattat ttaaagcttc
    26521 gatgtattca tcatcatctt tgtataattt tcttaaaata ggtgacaatg cgtaagtcac
    26581 agatgcacct tgctgtgttt gataattaaa ggtacaaact gacatcaacc aacgatagtt
    26641 cgctttccta agatcctttt tcgttacttg atatttttcg ctcattttat tcatcctccc
    26701 caataaaccc aacatcagaa gctcccgcag aagcagtgat ttgtgcatct ttttcagctc
    26761 tatgatacac attaaatgct aaagcgaatc ctatgatcgc cgctccaata ataggtacct
    26821 tcaaaaaagc ggctaagaaa aatccaacaa gtaaataact aatgtatcga cctgtaggca
    26881 taaagcgtaa caacatcgca ataccaacag caggcaacat ttttccggct aatgttagcc
    26941 catccataaa ccaattaggt aaaacatcca aaacgtaatt aacagcagac tgtcctaaag
    27001 taacggcaat aaatactgga attgctgcag ataaaccaaa gaaaattggc cccaaccaca
    27061 aaacactatt cattttctta aattctcgtt tattcgcata ttcctgtgat ttgcgaacaa
    27121 caaaaccatt taagattttg acaattacat ctagttgcac ccctaacata cctacaggta
    27181 gtcctattgc taatccggct tctactccaa gctggctacc aaatgccgtt gcaataattg
    27241 cgcacgttcc atagtccggt acagatgagc caccaaaacc ggcaactcct aatgacatca
    27301 attgaactgt accgccaact atcaaacctg tttttatatc acccatcaaa attcctgtga
    27361 ataatcccca aaaaactgcg taatttacaa agatcatcgg aatcccataa taatcaacat
    27421 gtttaataaa tgctaatata gtcagcataa taatttgaaa aatagtaaaa tgcattatcc
    27481 ttactccttt atcctaattt tgattgaatt tcctgtgcct tatccgccgg aacaaattgt
    27541 gtaacaattt ctacaccaga atccaacaat tttttatacg tgctgatatt ttcatctgat
    27601 gcataggctc tagaagatag ctgtaatgca ttatctttaa caacagtacc gccaacaatt
    27661 agtcggtcaa ttctaatacc gttatctact aaatgaagga ttgtttccgg ctctttcacg
    27721 atgacaaata ctttttcctg atcgtatttc ccctttttaa aattcgcaat cgctgtttct
    27781 tcagtgataa ttgatacagc cattccagca ggtctagcta aacgcatact tgatttttgg
    27841 atgtcgttat ttgcgacaga atcatcgatc accatcactc tgtttgcttt agacattggt
    27901 gcccattgcg tgacaataat cccatgcaat aaacggttat caactctcga cataacaata
    27961 ctcataaata cttcctcctt tatctttcat tttcttacaa gcaattacca tgccaaaaca
    28021 aaaagaactt gataaatcaa gctcttttta cataatcata aacgtattta acctcatgaa
    28081 tcgggatgtc gacactataa taatgttcaa cgacactgaa agattgctta aaatgtgtct
    28141 gaaatttttg aaattcctca ctcgtttcat tgaaataaat ttcatcttcc aaagtatttt
    28201 gttttactaa tcgttcaatt aagcaactaa tgtgaacata taatccgaaa cacgtattat
    28261 tatcaaatga aattcctaga tcattttgta agatgtatat agcatctgaa acccgttcta
    28321 ataatttagt cggatttaat atggtcaatt gctccattaa attcgttaga ctaaaattct
    28381 tcagaatatt attgttcaaa actttcagtt cattatcatt aaagtgtcta ttcaattgat
    28441 tggagaattt acttatattc attcctaaaa tcatatcttc aattgaaaag aattctacat
    28501 cttctagatg tggatttagt gttccaacaa taaaaagaat attgttctct ttacgaagct
    28561 ccgataaata gttttcattc actaaagagt aatagtcaca cgtttctatg acaagatcag
    28621 tatcccttgg aaagctatct tcaagtatct tttttaattt aaacgaagtt cctaatccgc
    28681 ttgcacaaga acagacaatc atatccttct taatatttga tgattcataa atataatcca
    28741 cattttgatt ttcttttact tgttcaagaa tctgtttaag cggtaaatct aataatatct
    28801 gatttgcaac atcaatagcc attcttgttg aaacattgtt tagtagtccg aatttttttt
    28861 gagcgtctag ttccaaattt agataaattt tctccagtga acccatatca acaagtaaaa
    28921 taatttcttc atatccggta cactcttgta agtatctatt aattttgtct gcaatctcac
    28981 tacttgaagt cattagtggc atatcaatgg cgtcgtatat atatctctca acgatcctat
    29041 tagcagtact tgctatacag gttgccgtta gatctccatg agccaaaatc agtgctaaac
    29101 gtttttgctg aaattcttta ctacacatta acttaaataa aataaccaaa acattttttt
    29161 gaatttttgt taaataaata tcccctattt gaataaaacg attgactaat tgaacttctc
    29221 gactacacca actattaata tcattgatga aggaatataa tttatcgtcc gatgttaatt
    29281 ggatttgata aatccaagaa ttcaaaaaga tagacgttat ttgaagataa taatagtcaa
    29341 attctatttg aaaatctctc tctatttcct taacatactg tctaaatcga atctcttgat
    29401 aggtgggata tgttaaagct gaattatttt ggatatcaac attcttttgt ctaaaatatc
    29461 tatcatattt ctcaaaaagc tgctgcttat cagtgtcttt cttccacgtt tgataaagat
    29521 actctataag ttcgttgata ttaaaaactt cgtctacttt ttccaaatca tccatttgaa
    29581 tatattttaa ttcattctgt cttttcataa ccttcatgcc gttgaaattc acccattgtg
    29641 gtaaatgata catctgaata taaagttctt ccaaatcatc taagaaataa aaggcattgg
    29701 cacaggtcaa ttgaatgata tttttcaaat tgccaacatt ttcactaaag cgatagttta
    29761 ataaaaaatc taataattgt tgagatatca gtattttctt tgctagtctt tcttcttctg
    29821 atttaaaaaa tatcttgatt aactgttttc tttcataaag agaacgactg tctaatgttg
    29881 gaagtgttag ctttacaggt attcttctca acaaagttga taatagttta tcctcaggtg
    29941 gttctgatgt tgcaaatata atccagcaac ttgatgtgta ccagttttca ttatcaccca
    30001 tccgatgaaa tttcctcgta tccataaact gaaaaagttt ctcctgacat tcattgctca
    30061 atgcatgaat ttcatctaaa aataagactc cattattagc taattctgct aaaccctttc
    30121 ttccatcttc ggctcctgta tatgcacctt ttacatgccc aaaaaaattg gacaagaaaa
    30181 gttctggatt attggcatat tcactacaat taactgaaat gaaagatgcc ttctcttcta
    30241 tataattatt ggccaacaaa tccttataca cataatttgc taaatagctt tttccagttc
    30301 cagtctcgcc atgaagtaaa atgggtaagc catttggcgg atagtagatt gctgctttaa
    30361 tttgagcaat tacatttcgc agacttcctt gacttccgat aaccctttct attccaaccc
    30421 taaaagagct atcataccat ttttccgctt cctctgaaga gctaaaaatt tgttgatttg
    30481 gaactttctc cggtaaagaa aagaagatta ctggacgagt attgattttt ccaaattctc
    30541 catcttttac gaattcattc aaatattgag agacaatatt acgactaaca tttaattttt
    30601 cagatatttt ctgtgctgta ttgttccccc atttattttt aatctcagag ttctctatga
    30661 cataattgac aatgcgttct tttaattctt ccatcacagc acctcctcga cactattcga
    30721 attttgccga cagtattttt ccataaatat gacttgctaa aaaggctttg tttagatcat
    30781 tcaattggaa cgttgcttgt tttgcttgat ccgctaagac gttgaaaaga tactctggcg
    30841 aaatcgcata ccccaaaata tcaacgattg ttgcaatcag atcttctttt gattcttcat
    30901 ctgaaagtaa ttcacgatac aacatcgttt gtttgcttac tgtgtcatat tcctctaagg
    30961 attcatctgc taaaagaacc acttgttcca acaaacggtc ggataaatat ttatagaaaa
    31021 tcagccccaa taaataattt ttatattctg acgcatccat tttactacgg agattatctg
    31081 ccgcactaaa taacttctga tttaattctg cacccaaaaa gatacactcc tatctcttat
    31141 ttaaagttgc tatataccct cttattttag catactcagt tttagaaaaa gcaaaaaaat
    31201 aaagaaactt attcttgata acaagctacg tgacgtatat ccctaacaca aaaatagcca
    31261 tatcctttgg tttggatacg gctatttgat attactcatt caattatcaa atcacagtag
    31321 gtgtgaatct catcgggatc ggcaatatct acttctgatt aggatcaaag gtgattttct
    31381 caataaaggt gtgatacaaa gctttaactt gcttcttctc actctggata agaaagcaat
    31441 ccaaactagt caatatctgt tggacatctt tgacttgaat cggttcccct tgttgttgaa
    31501 agatccctaa gatttcattc tctcgaattt gtaactgtct tcgtttttca gtcaattcat
    31561 ttaaacgatc ttttaacatc ggaaacaatt cgggactatc ttccaaagca gcttcctgtt
    31621 tttgctctaa tagttagatt ttgcgtacca ttttcttctt ttattttcct tcgattgctg
    31681 attttaatat agtctaaagg aaatttatat ttattgtatt ctttataact tcaagagaac
    31741 ttcgtttgag tattactgta tttttttaat tccataagaa aattagttat tacctgccac
    31801 ctaatcagca aatctgaacc tgagtcaata taattaatat tattttccat ctccaacatt
    31861 gtaagtactg ttggtgttaa tatagggact ttgtacaatt gtattttttt gattttatca
    31921 tctgcacaaa ggagattttt taaatctaat ccttctttta tttcaaaagt tgaatttttt
    31981 aaatctataa gagtaaaacc aattccttca tactgaattt ctgaaaatcg aatttttctc
    32041 ccagttgata gatcaagggg cagaaaacct tttattacat tgtgagtgtc accatcattt
    32101 acaatgtcat taggaatttt gattttagaa ttctgtggag cacctattgg aagataataa
    32161 ttcattataa ttttgtagtc ttcatgagga aataaagtac ttttaggatt gctaagatat
    32221 tctcgtaccg aatctaagaa tggcttatcc attgaatctt catccccatt taaactaaaa
    32281 aacattccta caattgcttt tgcaactcta agtgaattca ttttctttat tgaatgtgtt
    32341 aaatcagact taccattctt taaacttgta ttgttataaa ggaaattttt attgtcttct
    32401 gcaaatctaa tatattcctc aacatactca ctaccaaaaa aactattaca tttactacat
    32461 aacgtattgg aatggcaaca gtttttttga caaattttat aaggtgcaga acttctttcc
    32521 gtatgctatt ccgattgtcc ttgacaatga gcctcctcgg atgtgacgat cttcggcagg
    32581 agctaacagt taaagttaga ctgcctcgct agcgttagca catccgagct cattatcaag
    32641 gattcgctgc gccaat
//
LOCUS       DACDTH010000028        32158 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDTH010000028 DACDTH010000000
VERSION     DACDTH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.028"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(96..476)
                     /gene="yajC"
                     /locus_tag="IWK54_001385"
     CDS             complement(96..476)
                     /gene="yajC"
                     /locus_tag="IWK54_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9593511.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(573..1718)
                     /gene="tgt"
                     /locus_tag="IWK54_001386"
     CDS             complement(573..1718)
                     /gene="tgt"
                     /locus_tag="IWK54_001386"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9593512.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1936..2634
                     /locus_tag="IWK54_001387"
     CDS             1936..2634
                     /locus_tag="IWK54_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9593513.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2802..5441)
                     /locus_tag="IWK54_001388"
     CDS             complement(2802..5441)
                     /locus_tag="IWK54_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9593514.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6060..7091)
                     /gene="queA"
                     /locus_tag="IWK54_001389"
     CDS             complement(6060..7091)
                     /gene="queA"
                     /locus_tag="IWK54_001389"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9593515.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7284..8459
                     /locus_tag="IWK54_001390"
     CDS             7284..8459
                     /locus_tag="IWK54_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9593516.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8463..9104
                     /locus_tag="IWK54_001391"
     CDS             8463..9104
                     /locus_tag="IWK54_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9593517.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9101..10018
                     /locus_tag="IWK54_001392"
     CDS             9101..10018
                     /locus_tag="IWK54_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9593518.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10015..10686
                     /locus_tag="IWK54_001393"
     CDS             10015..10686
                     /locus_tag="IWK54_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9593519.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11227..11715)
                     /locus_tag="IWK54_001394"
     CDS             complement(11227..11715)
                     /locus_tag="IWK54_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9593520.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11916..12182)
                     /locus_tag="IWK54_001395"
     CDS             complement(11916..12182)
                     /locus_tag="IWK54_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9593521.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12182..12445)
                     /locus_tag="IWK54_001396"
     CDS             complement(12182..12445)
                     /locus_tag="IWK54_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9593522.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(12438..12710)
                     /locus_tag="IWK54_001397"
     CDS             complement(12438..12710)
                     /locus_tag="IWK54_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593523.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            12921..13106
                     /locus_tag="IWK54_001398"
     CDS             12921..13106
                     /locus_tag="IWK54_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9593524.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13248..15071)
                     /locus_tag="IWK54_001399"
     CDS             complement(13248..15071)
                     /locus_tag="IWK54_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9593525.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15669..18374)
                     /locus_tag="IWK54_001400"
     CDS             complement(15669..18374)
                     /locus_tag="IWK54_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9593526.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18680..19627)
                     /locus_tag="IWK54_001401"
     CDS             complement(18680..19627)
                     /locus_tag="IWK54_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9593527.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19817..20086
                     /gene="rpsN"
                     /locus_tag="IWK54_001402"
     CDS             19817..20086
                     /gene="rpsN"
                     /locus_tag="IWK54_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9593528.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20262..20624)
                     /locus_tag="IWK54_001403"
     CDS             complement(20262..20624)
                     /locus_tag="IWK54_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9593529.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20635..21756)
                     /locus_tag="IWK54_001404"
     CDS             complement(20635..21756)
                     /locus_tag="IWK54_001404"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9593530.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21772..22122)
                     /locus_tag="IWK54_001405"
     CDS             complement(21772..22122)
                     /locus_tag="IWK54_001405"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9593531.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22252..23763)
                     /locus_tag="IWK54_001406"
     CDS             complement(22252..23763)
                     /locus_tag="IWK54_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9593532.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24085..25437)
                     /locus_tag="IWK54_001407"
     CDS             complement(24085..25437)
                     /locus_tag="IWK54_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9593533.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25590..26666)
                     /locus_tag="IWK54_001408"
     CDS             complement(25590..26666)
                     /locus_tag="IWK54_001408"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9593534.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26862..28607
                     /locus_tag="IWK54_001409"
     CDS             26862..28607
                     /locus_tag="IWK54_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9593535.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28887..29324)
                     /locus_tag="IWK54_001410"
     CDS             complement(28887..29324)
                     /locus_tag="IWK54_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9593536.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29308..30681)
                     /locus_tag="IWK54_001411"
     CDS             complement(29308..30681)
                     /locus_tag="IWK54_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593537.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31003..31491)
                     /locus_tag="IWK54_001412"
     CDS             complement(31003..31491)
                     /locus_tag="IWK54_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9593538.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31513..32115)
                     /locus_tag="IWK54_001413"
     CDS             complement(31513..32115)
                     /locus_tag="IWK54_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9593539.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9191 a         6877 c         5938 g        10152 t
ORIGIN      
        1 caatatatcc catgctctta tctctttctt ttttgaataa aaaatgaggt aggacatctt
       61 tctgccctac ctcgtcttta aataagcttt gatacttatt tttcttcctt ctcttctgtc
      121 tcttccagcg tttctgttgt ttcagttttt acttcaactg tctcagctgg ttcttctgtt
      181 gtttcaacag tagtcgttgc atcatttgtt acgacagtcc ctggtttgac cgttttgatt
      241 gctgcacgat cgtactcaag gaagatccct tcgcagtcta aaacaactgt ccgtttgtca
      301 ttgtcgattt ctgaaacaac tccatgtaat ccgccgatcg ttacgactga atcacctggt
      361 ttcatgctat ctaacaagtt ttgacgttct tgctgttgtt tcttttgcga acgtgacata
      421 aaaaaccaca ttgctaatag cgcgataaac atcacaagca ttggtaatgt tcccattttt
      481 ttcacctcac taattctgta tctacaaaaa acactttagc agaaaaatgc tgataacact
      541 agttttttac ttaattttta aaagattttc ctttagaagc tttttgcgtt ttctttattg
      601 aacccgtatt cttcaaaaaa ggcttctcta aattcaagca aattatcatc catgattgct
      661 tgacgtactt gtttcattag attcaacaag aagtataaat tgtggtaaga agtcaaacga
      721 atcccaaatg tttcatcaca tttgatcaaa tggcggatat aggcacgtgt atagttttta
      781 catgtataac aatcacattt ctcatcaatt ggacggaaat catgggcata tttcgcattt
      841 ttcactacta gacggccttg agaagtcata catgtgccat ttcgagcgat tcgagtaggc
      901 aagacacagt caaacatgtc tactccacgg ataacaccat cgatcaatga atcagcagca
      961 ccgacaccca tcaagtaacg tggtttgttt tcggggatca acggtgtagt gaattctaat
     1021 acacgattca tttctgattt aggttccccg acagataacc cgccgattga gtaaccaggg
     1081 aaatccatcc cgactagatc tttcgcgctt tgacgacgaa gatcttcaaa ccctgctcct
     1141 tggatgattc caaataatcc ttgacggtct ggattagcat gagctttcaa tcctcgttct
     1201 gcccatcgag acgtacgttc tactgatttt ttcacgtagt cgtaactttc atcgaatggt
     1261 ggacattcgt caaaactcat catgatatca gagcccaatt tattttggat gttgattgct
     1321 ttttctggtg ataaaaacat acgcgaaccg ttcaagtgat ttttgaaatg cacgccttct
     1381 tcttcgatat tccgcatatc acttaatgaa aaaacttgaa agccgcctga atctgttaaa
     1441 atcggttgat cccagttcat aaatttatgc agtccgccag cttcttctac taagtcttca
     1501 ccagggcgta gccataaatg atatgtatta cttaaaataa cgccagcacc catttctttc
     1561 aactcttctg gggacattgt ttttactgta gctaatgttc ctactggcat gaacattggt
     1621 gtggggaaag ttccgtgtgg cgtgatcaat tcccctaaac gcgcgccagt atgtttttct
     1681 ttcttgatta aacgatagcg aatggcaggt tgcgtcattc atttccctac tttctatata
     1741 aaatatcttg acaactccca ccatcataat cgtttcaagt gctttttgca agttccctcg
     1801 gcttccttct actttattta atattctttt ttttatcctt tttttatgaa taacaaaaaa
     1861 aagaaaaaca ttatgacaga aatatttttt gatacatggt atattggtgt tattaaagaa
     1921 aaggagggaa aaatcatgca atcttctgca gaattaaaac gccaagcaaa agagagtcta
     1981 aaaggacgtt ggggacaagc tgttttacta aatctaatcc ctacattgat tggtattgct
     2041 actattcttc ttattatgat tcctattatc ttgtttgcca ccaccatata tgatggatca
     2101 gcgagcgtct cagaagcgac aacttcagga ggcggtagtt caggaggcgg tggcggaatt
     2161 atttccagca tcattagcgc tttgttcatg agcggtattt cttggactta tcttgatatt
     2221 ttacgtggac aaaaagatca gatcgaacca ttcaaagatg catttcgtgg attcaaaggt
     2281 ttgtttatcg gcggagtgat cagcttagct gtgctgatct cgatctttac aacattatgg
     2341 ggtttgctat tagttattcc tggaatcatt aaaagctatt cttattctca aagctatttt
     2401 atttactacg atatcgtcca agaaacagga caaacaccta aagtactgga tacgatcact
     2461 gcaagtcgaa aattgatgga tggttataaa ggacaacttt tctggttaga tctttcattt
     2521 atcggctggc atatcttagc attagcaact gctggtattg gttatctatg gctcagccca
     2581 tacatttctg caacaaaagc agcattttat gaacaattgc caaaagatat ttaaaagaga
     2641 gtgtgacttg tgaccgaggg ggtggcagga gaacaccgtt tatcgagagg cagtaaaaga
     2701 aacattcagc ccattagcaa aaaggaggtg tgacaacatt catgtcacac ctcctttttg
     2761 tatagttcca gtaaaaagaa aatcaactta gaaacagtta atcattcctc ttcttctgct
     2821 tccggtaatc gatggaactt gatcagttca ttcaccgcga ttggtacgat agataaaatc
     2881 cctaccaaga accagtgatt caaactgatg tgtactaggc cgaatagttc ttgcgtaaat
     2941 ggcagcaaag caacagccgt tgtaatagct aatgccagta acgccatttc taccaaagct
     3001 ttattggtcg ctaaattgac tttgaagatc gattggctgc tgcgtacatt gaacacatgc
     3061 agaatcgatg aataagctaa gatcaagaat gcaacagttt gaccgacatg ctgacttgcg
     3121 tctacccatg ggctgatagt atcgacataa ctacctaaat aataaccaaa caaggttacg
     3181 atggtgaaga cccctgcatt tatggcaatt ttttgcaata aacctcgagc aaagatccct
     3241 tcatttttcg gaatgggtgg ctgatccatg atgccgtgtt ccgctggctc tcttcctaaa
     3301 gcaaatcctg gaagtccatc agcaactaca ttgacaaaca gtagttgcac agctgtaaat
     3361 ggtgctcccc atcctaacag catcgcaatc aaaacaataa agatctccga aatattacag
     3421 cttagcaaaa agttgatagc tttacgaata ttctgataaa ccgaacgtcc ttgactgaca
     3481 gcgtctacga tcgtcgcaaa gttgtcatcc gtcaaaatca tatctgcggc actttgcgcc
     3541 acatctgttc ctgtgatccc catggcacac ccaacgttac ttgctttcaa cgctggtgca
     3601 tcattcacac catccccagt catcgctacg actgcacccg tacgttgcca agatttgacg
     3661 atccggatct tgtcttctgg cgttacccga gcatagacag agagagcttt gacacgcgca
     3721 tctaattctt cgtctgataa ttgatgcagt tctgatcctg aaagtgcttc tgacggatca
     3781 ttcaagatac ctaattcttt cgctatggca ctggcagtca ccacatgatc tccagtgatc
     3841 atgactgttt tgatgccagc ctttttcgct cgcttgattg ctcctttact ttctggacgt
     3901 ggtggatcga tcattccgat cagaccaatc aaacgtaagt ctttttccag tgcttcagaa
     3961 gaaatatctt gcggttcttc gtcataagta gcataaccaa cagcaatcac tctcagtgcc
     4021 cgtttaccaa agcgatcatt aacgatcgcc gcttgttcta catcgcctga actaaagcgc
     4081 ggagcaagaa catcgaatgc tcctttggtg attgagatgt atttttcgcc catttggtga
     4141 actgttgtca tcatttttcg ttcagaatca aaaggaagct cgtttactcg tggatatttt
     4201 tcttctaatt ctgcctttgt atgataattc tcttcaacag cccttacgat ggctacttct
     4261 gttggattac ctttgataac aaggtcgtca tcttcttgct cgaccgtcac atctgtacat
     4321 agtgcagcca ttttcaatat ttccatcgcg ctgttcgtca ttgaatcttc gatattcgtt
     4381 acttcatctt ctttgctcca tacccggcgg acgcgcattt cattttgtgt cagcgttcct
     4441 gttttgtctg agcagataac attagcggtt cctagcgttt caactgctgg caactgtcgg
     4501 atgatcgcat gtttcctcgc catcttctgc acaccgtatg caagtgtcaa cgtaacaata
     4561 acagtcaagg tttccggtac agctgcaaca gccaaggaaa tcgaagtcat gaacatctca
     4621 aggagtggtt ctccttgcag ttcaccaatc acaaatacta aagccgcagc cgctaaagcg
     4681 atcatactga ttcgtttgcc tagctgattc aatcggacct gtaaaggtgt tttttgcata
     4741 gtattttcgt tcaaaagatc cgcgatctta cccatttctg tagccatgcc aatggcagtg
     4801 acgatcgctt tcccacgacc tgcagcaacc gtacatcctt taaataccat attaagttgg
     4861 tcacctaatg actcatcttc ttcagcaata aatgctgcat ctttttcaac tgcttcactt
     4921 tctccagtca acgccgattc ttctacacgt aattgtgatg cttcgatcaa acgggcatcg
     4981 gcagaaatag catcaccgga ctctaaaaca agaatatcac ccactaccag ttgctctgac
     5041 tcaacagtgg tcactttgcc gtctcgtatg acagttgccg tttgtttatt catatcttgc
     5101 aaggattcca gtgctttttc tgcgtttcct tcttggacaa tcgccaatac cgagttgatc
     5161 acaacgatag cgatgatcag cagtccttca aataaatccc catgttctgt agcaaatgcg
     5221 gtataaaagg aaatcaccgc tgctacaaga aggatgatcg ttgtaaaatc ggacaagctt
     5281 cgaaagaatt ttttgatcat cgattctttc ggcgcctcat cgaatttatt caatccctgc
     5341 ttttttcgcc gttcatccac ttcttgtgaa gacaacccag tatctggatc tgcttctgtt
     5401 accttcttta cttcttctat tgtctgcttg tattcttcca tcctattcct cctttaaaat
     5461 cttcgtacaa aaaaaggtac ttattttact ataacatcct ttttttattt acttcaaata
     5521 aagggcttat cgattatttg ttaagaaagc gtaaaattta aagtgcttat atttgcaaac
     5581 gctaccaaag gatgctatta tatagatgta aggaaggagg ataccatcaa atagacagaa
     5641 gaatttctcg atagaacagt gtaagcaaac aaagttgtta gaagtacagg tttcacccag
     5701 acatgcgtgt agagtacaaa cctataaata gtgaatgcgt atggatgttt ggatcacgaa
     5761 gtatccgaaa caagaagtaa tgcgatacgt aacaattgaa taacgtaaac gtctgaaaat
     5821 tgaataggaa caagagatgt ttcaaatgtt gaagatgtaa cgaaaacaaa atttttgttt
     5881 aacatgaaga aattcaacac cgtgctttgc atgtatgatg gaaagttaca atcatttctg
     5941 tttatttgaa tccttccgag gtacaagaaa gccgagctta tcgagagctc ggctttttct
     6001 ttgtcataaa atctttgtaa agcttttttg ttattcaaaa ctagcggctt ttcataacct
     6061 tatttcacga acatcgcatc tccaaaacta aagaaacgat agcgctcttc aatcgcatgc
     6121 tggtaagcag ataaagtcaa ttctcttcct gcaaaggcac taactaacat gactaaagtc
     6181 gattttggca aatggaaatt agttgagaaa gcttctacca ctttgaattc atagccaggc
     6241 gtaatgaaaa tatcagtcca tccgctgtct gctttgattt ctccattaaa tttcgttcca
     6301 atcgtttcta atgttcgaat tgaagttgtt ccgacagcca cgattttccc gccattttta
     6361 cgtacttggt tcaatcgctc agccgcttct tccgtcaaac ggtagaattc actatgcata
     6421 tggtgttctt caattttatc tacgctgaca ggtcggaatg tacctaaccc tacatgtagt
     6481 gtcagataga ctaattcaac accctttgct tcgatctcat caagtaattc tttcgtaaaa
     6541 tgcaatccag ctgttggcgc tgcagcagaa ccattttctt tcgcatagac agtttgatag
     6601 cgatctgggt cttccagacg ttctttgata tatggaggca atggcatttc tcccaatgat
     6661 tcaagaatct ctagaaaaat tccttcatac tcaaagtcaa tgatccgtcc gccgtgttcc
     6721 aattcttctt tcactactgc ttgcaatctg ccatcaccaa aagagattct tgtcccaact
     6781 ttggcacgtt tcgcaggttt gatcaatgtc tcccacgtat ctccttgtgt attcgttaga
     6841 agaaggactt ctaaatgcgc accagtatct ggtttttctc catataaacg agcaggcaga
     6901 actcgtgtat tattcataac tagcgcatca ccaggctgta actcttcaat gatttcatgg
     6961 aaatgtttat cttccatctc tccagttttc cgatccaaaa caagtaaacg agaatgatcg
     7021 cggtctttta atggtgtttg tgcgattaat tcttccggca agtcaaaatc aaaatcttct
     7081 gttgttaaca tatcgtcact cactttctat catgtgcttc atcattctat catgtttttc
     7141 gttctgttgg caaaatcagc actttttcac ttctattttt tgttttccta aataaaaaaa
     7201 ctaccctatt tgtatttttt cattgtttta ggcatactag taatagatag ttcttcattg
     7261 aaaatagaaa aagaggtggc accatgattg aatttcaaaa tgtgtcaaaa atttataaag
     7321 gtggaaaagt cgctgtagag gacgtcaatc tttcatttga aaagggtgag ttcatttgtt
     7381 ttatcggtac gagcggaagc ggaaaaacga cgacgatgcg gatgatcaac cgaatgactg
     7441 atccaacaca agggaaaatt ttgattgatg gaaaatccat ccaagaaatt gatccagtgg
     7501 aattgcgacg tcagatcggc tatgttatcc aaagtattgg attgatgcct cacatgacga
     7561 tccgtgaaaa tatcactctt gtccaaaaat tgttgaaagt cagtcaagaa gaacggaata
     7621 aaacagctga aaaaatgatt gatcttgtgg aattgccccg tgagatgtta gatcgctacc
     7681 cacatgaatt atctggtggt cagcagcagc gtattggagt cgttcgtgcc cttgcagcga
     7741 atcaagatat catcttgatg gacgagccat tcggtgcgct tgatccaatc actcgtgatt
     7801 ccttgcaaga ccttgtcaaa gatttacaag agcgtttggg aaaaacgatc gtatttgtaa
     7861 cccatgacat ggatgaagcg attaaactag caagccgtat cgccatcatg agtgaaggcc
     7921 gtgtgatcca atttgataca ccagaaaata ttttacgtca tcctgcaaac gatttcgtag
     7981 aagagttgat tggagaagat cgcctactac aagccaagcc aaatgcgaca agagtcggcg
     8041 aagttatgct aaatacagca atcacgatta caccggaaaa atcattacaa gaagcaatca
     8101 aactgatgag ggaaaaacgt gtagatacgt tgttagttac cgataattcg aatgtattaa
     8161 aaggatttat cgatgtagaa acgttgaata aaaaacgagg aaaagtatcc agtgtcggcg
     8221 acattttgaa taaacatgtt ttttacgtaa aagaatccgc ctatcttcgt gataccttgc
     8281 aacgcatttt aaaacgcggg ctgaaatacg tgccggtcgt tgatgaacag aacaaagtcg
     8341 taggtatcct gactcgtgca tcattagtgg atatcgtcta cgatgtgatt tggggagaag
     8401 atcccgcaac tactgatgtt gcagagtcag ctactcctga agaatctaag gaggtctgac
     8461 ctatgcagca gttttttcat cagcacggtt cagaaattct ctcaaaaggt tttgaacatc
     8521 tttatatttc aggacttgct ttgatcattg gtatcatcat tgctgtaccg cttggtgtct
     8581 tactgacacg ttttccaaaa gctgccacga ttgtgattgg cttaaccagt gccttgcaga
     8641 caatcccttc tcttgcttta ttggcattga tgatcccgct atttggcgta ggtaaattac
     8701 ctgcgattat cgcactcttt atttattcat tattgccgat tttacgtaat acgtacattg
     8761 ggatgaaaaa tgtcgattgg aattatcggg atgtggcaaa aggcatgggg atgactgagt
     8821 tccaatccat tttttccgtt gaattgccca ttgctatgcc aacgatcatg gctggtattc
     8881 gtttagctgc cgtttacgtg attgcatggg ctacacttgc ctcttatatc ggcgctggtg
     8941 gtttaggcga tttgattttt agtggactga ataattacca accagatctc attttggcag
     9001 gtacgatccc tgtaacaatt ttagcactat tggctgattg gcttctaggt ctcttggaac
     9061 accggctgac accgatcgca cttagagagg agaatgaaga atgaaacgta tactaaaaat
     9121 gaccatggtt cttttctctc ttcttatact tgcgagttgt tccttccctg gtcttgccag
     9181 caatacagat gaagacacca tttcgatcac aggtggaatc acttcagaag cacagatctt
     9241 agcaagtcta gttgccggaa tggttgaaca ttatacagat aaaaataccg caatcatcaa
     9301 caatctggct actacaacta tcaaccatca agcgatgatg aacggagatg cttcaatttc
     9361 agcggctcgc tacacaggaa cagatttaac gactacattg aaccttcctc ctgaaaaaga
     9421 tccgaaaaaa gcgtttgcta ccgtgaaaga tgaatttgag aaaagatacg gtcaaacgtg
     9481 gttcccgtcc tatagttttg aaaatacgta tgtctttctt gttcgaaaag acaccgcaca
     9541 aaaataccat ctatctaaag tcagcgatct gaaaaatgta gcggatgaat tagtagctgg
     9601 tgtagatact tcttggatca atcgtaaagg agacggttat gacggcttcc aagaaactta
     9661 tggcttctcc ttcaattcga tattacctat gcagattgga cttgtttatg acgcagtcga
     9721 agcaggaaaa atggatattg ttcttggcta ctctacggat ggacggatcg ccagttatga
     9781 tctagtcatg ttggaagatg atcgtcaatt cttcccacca tatgatgctg ctccagttgt
     9841 agacaacaag atattagctg atacgcctca tttaaaagaa gccttaaata agttaggtca
     9901 taccatcagt acggaaaaaa tgcaggaatt gaactacgaa gcagataata atctagtgga
     9961 accatcagta gtagcacaac gtttcttaca agaaaaccac tattttgaag ggaagtgaag
    10021 caattggata aaatgaatgt ctttcaacaa tttttctatt atttccagga aaatggctcc
    10081 tatatctttg cacaatttat ccgtcacttc ctgatttcta tctatggtgt cctctttgca
    10141 gcagttgtcg ggattccggt tgggatcatg atttctagga gacgtaaatt agcaaactgg
    10201 gtgattcgtt tggcgaatat catccagacg atcccttctc tagccatgat ctccatttta
    10261 atcattggat taggcctggg agtcaatgtc gtgattgtta ctgtattttt gtattccctc
    10321 ttaccgatca tcaagaatac gtataccggg atgatccaag tagacaaaaa catccttgat
    10381 gtcggcaaag gcatggggat gactgctaga caacgactat tcatggtaga gttaccactc
    10441 tctgtttctg ttatcatggc aggcataaga aacgcgttag ttgttgccat cgggataacc
    10501 gcgatcggtg cattcgtcgg tgctggtggt cttggagata tcattatccg aggaaccaat
    10561 gcaacagagg gcacatcgat tattttagca ggtgcattgc caactgcatt aatggcgatc
    10621 atcacggact ggcttttagg tattttggaa agacggctag atccagccag ccgtacatca
    10681 cgctgatgtt attgtaaaaa acaaaagctt ctgcgaccat ttgttggtcg caaaggcttt
    10741 tttagttctg tcatttttaa ggcaataatc cactctgatt cttccctcct tatgggtaaa
    10801 gagttgcttt tcttagctat ttatccattc cgtatactcc aacttcatac tgccaacggg
    10861 ttcatatctg catccaccga ctaagttttt ttcttatccg ctaaggcatt ttgccaatcc
    10921 attttcccat cactcgtcat ggaattcata ccacatgatc tctaaatact cgtctgcttg
    10981 ttctttgaat tgggcatcaa ttcttacatc ctttggcgtg gctttcattt aagccttttc
    11041 catcagtcct cgattgttcg atgccttcag ctgcatcatc aaaactggtg actgccgtat
    11101 cggtaacttc gatcatcttt tatacaatcg ggaacttgcc atcctcatcc ttggctaaaa
    11161 acatatctag ccattccagc ttctcgccat gggaggtttg cggttcctaa aaccggtaca
    11221 cgattcttat ggactgcatc cgtcgcaact agacttggcg atacgatgat ccttcaccag
    11281 aagagcaccc ccgtaaacca attgatcgat atattgccaa taagaaaaga cattggcgta
    11341 aaagatatcg atccctcgtg atgcattgcc gttgatactg ctattcatgg ccgaaagcgc
    11401 cacgacttta acttcttccg tttgagtgac attcatgttc ggcaatgtcg ctttggatac
    11461 ccgctctgct aaaggaacga cactggtatt gtatttcgta tctgtatctt ttccaaaaga
    11521 ccatgccaac gaatcctctg ggaaccaata agacgactcc ggttgattgt ccattgcttt
    11581 tttcaacata cttttggctg tttctcgata aacgacattt ccaattgtct tcgcctcact
    11641 ttggcgataa tcgatggtcg ttgccactgc caacactaac aacgatgctc caattttact
    11701 gattttcctt gtcacacttc ttcctccttg ttttacataa acccgtcaac attccattcc
    11761 taaactcaaa caatgaccct tcaaaggtgg agatatagga acaaattaaa gaaatcatgc
    11821 aataaatcca agaaggttgg ttttagaaca aaaaaagagt ctcctttttg gggagactct
    11881 ttgttaacta tctaaaaagg ttggctcttc tcacttcact cataatgtgt ccaggcagaa
    11941 taaatttcta caactttatt ttcctcatca actttgtaaa ccactcgatg ctgtctattc
    12001 agcctgcgtg agtatctgcc ttcatgtgta ggtctcagct tttcaaaaga ctgcgtgggc
    12061 atatatggat cttccttcaa tgtctgaatc acttcttcaa attgtttctt taaattcgtt
    12121 tgcttgattt ttcttaaatc cactttcgca gagtttttga ttgcgactgt atagttactc
    12181 atcaaagagc ttcccaatca atatcatcga tattcgtaaa gctactgtcg tctttttcgc
    12241 ggtctcgtac tttatctaaa gtacctgttt gctcaagaaa aagggtttct tgaatcgctc
    12301 tccagtcttc cagcccaatc aatacagcat tgtttccact acgatcacta ataatctcga
    12361 tttcagtatg attttcattt acctctttca ataactgata aaaattttta cgagcagtgc
    12421 taggattcat cgttgtatta gccattgtct ctcctccttt ttctgatata attatagcgt
    12481 acttgattaa gtacgtcaaa aaaatacaat aaagcactta aattcagcaa acttttcctc
    12541 ttttttacat agagaaaaag accccttttt agggaggatc tttgtccacg ctcacttctt
    12601 actcctttac gactagcggt agccaaacgt aaagtaattg gtaacaacgt atcatcttta
    12661 gcaaaggtcg aatccaaagc ctgtgagtct atgcaacaac accaatacac gaaggttcct
    12721 cgttgcagtg tgacttgatt gatatcttga ataacctgag gatacgcacg aaagatttgt
    12781 aacggttatt caaaactgat tttggccgtc aagtcttcta ctgcctcaaa aatgattgcc
    12841 tctttcgtct cctgtcattc atgttcacta tcgatttgat acttgcttag accgttgggt
    12901 ttgtgaatca ccaattaaaa atggcactga gttatggtaa caaaaaatgg tcacttgacg
    12961 aggctgagat tcctacttat gaagacacat ggcacttaag agtcgaggta aaggaagaag
    13021 ctgaaaatat aggaggctgt acattatttg gcaaaggatt cggaaattat gatcaagaca
    13081 tcaaaatcaa aatgttttac agctaaatag gaagaataat aacatttcat acgctgttcc
    13141 catctttaat ggcttataga gggcaccttt agctgagaat aagacaaaac agaccctcta
    13201 gcttcaatta agagctagag ggtctgtttt tgattttaaa acttggttta tttcttcaaa
    13261 tggtagctat aggtgctgac aacgatattt tcacgccaag acaatcgcaa tcttaaccgc
    13321 aaacttgttt ggttatggag aatattctgc cagcgtctgt ttggtacaaa ttgaggaatc
    13381 aacacagtag ttgtatagtt ttgtttcgct gcatttttac ttacgatatc tacataacgg
    13441 ataattggat tttcaatcga gcgataagaa gagtggacga ttgaaaaacg gacatctggg
    13501 aaatgctttt taaattctgc ttcgatttct ttttcttttt cgacatcttc gtctagcgaa
    13561 acatgcatcg caacgacata atcaccgatc gaacgtgcgt aattcaacgc accgatattt
    13621 acccgtgtca cattcccgac taatacaaga acggtattcc cgtcataatc atgtagtttt
    13681 gcatcatttt ctaaacgaag ctgttctgct acttttttat aatgatcatg gatcttataa
    13741 aaaatgaaca taacgattgg cataatgatg aagaaaggcc aaatgtcccc taaccgatag
    13801 acaaacaaaa tcgcaatgat tgcatatgag atgaacgcac ccgtaatatt ggcaatcgat
    13861 ttgcttaacc atctttttcc ttcttttttc catttgacga ccatccctgt ttgagataaa
    13921 gcaaatggaa tgaacacgcc gatagagtat aacggaatca agcgttctgt tgagccatgg
    13981 aaaataaaca gcaatacgat cgaacctagc gctagtgtga taatcccatt ggaatagcct
    14041 agtcgatccc cacgatcttg atacatgtga ggcatgaatt tatcttttgc cagattgtat
    14101 gcaagaactg gaaaagcaga aaaaccggtg ttagcagcaa ctgctaaaat cagcgctgta
    14161 gcaaattgaa ggacgtaata caaaatacca tgtccaaaga cagctttacc aatttgagac
    14221 aaaacagtga cttctttttc aggaacgatc ccataccaat agttgataaa tgtgatcccc
    14281 acgaagaaga aacctaagat caatgccatc atcgtcaatg tagcagcggc attttttgct
    14341 cttggcttct taaagaatgg tacagcatta ctaatcgcct ccaccccagt taaagaggaa
    14401 gaaccagaag aaaatgctcg taaaatcaaa gcgatagaaa cacccggcac agccgctcct
    14461 ggaagtgcag tagcttgcaa tggcacagca cctgtcacga tattatataa accaacaacg
    14521 atcagtaacg agatcacaag gataaatgtg tacaccggaa ataataggaa cgatgcagat
    14581 tccctcagcc cacgaagatt cagcatcatc agtagcaaaa cgattgtcac agaaatagcg
    14641 acttgatgac cgtagagtgc tggcaccgct gatgtgatcg cttcagcacc tgctgagaca
    14701 gatactgcta ctgtcaacat gtagtcgatc aataaagagc caccggaaat caagccagca
    14761 ttctttccaa gattctcact gctgacgaca tatgctcccc cgccatgagg ataagcatgg
    14821 atgatttgac gatatgatag cgttaatgaa atcaataaaa taataacaaa agcggcaatg
    14881 ggtagcgaat accagatggc tgcagcagac aaagcgacta aaacgacaac gatttgttcc
    14941 gtaccatatg caatagatga taacgcatca gacgacaata atgccaaagc agcaaatcgg
    15001 gttaatttgt gctcatcatt ttcagcagat tttaaaggct tacctactaa caaccttttt
    15061 aagtaatcca atttctaaac tcctttacac acaattttac ttcaagtttt atagtcgaaa
    15121 atttatagta aacaagatgt tatagtacgc tcactgacct taaaagtcaa taaactttaa
    15181 gaaaataatt ggaaaaaaac agaaaaatat caagcgcttt ttcaaacgct ttaatacagc
    15241 aaaaatctaa cattttttta tgggcttttt ctttgaattc tataataaaa ggatagaact
    15301 tgacttagaa aatcaagttc tatccttttt gatatcttaa cttttggaag agagatgtaa
    15361 ggctgctttc tacccatcat tcttactact cgaagcatag cgcagttgtc acaacctctt
    15421 gataaacggt gtttaaaagc ttgcatctgc ggtaataagc ccaaccaaat taaaaacata
    15481 agaagctgtt tttatttggt cgttcttatt acttgatgca gagcagcttt tttacaacct
    15541 cttggtacac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaaa cggcaaaata
    15601 tgaggaacta tttttccatt tttttctcta atactcaggt caaaacactg atttcacaac
    15661 ctcttttttt aatgaaaaat atggctctca tgcgccgctt ctttgaattt cgtcattttt
    15721 tcttcgatga acggatagat tgccgttcct acgattccaa agacaacaaa caaaccgatc
    15781 atgatccaga ttgctttcgt agcatttggc caataaattc ctcctgcaga ttcccgtaac
    15841 aagtttaccg catgagtaaa tggcaagaac ggattgatca tttggaagaa tttccctgag
    15901 acttggatcg gatagttccc gcctccgcca gagatcgaca gtactaagat gataatagcg
    15961 atccctttac cgacattatc gaataatgca gccaagacat agacgatcat catgaacgct
    16021 aaggcgatca agacagcaaa tgacacgctg tagaccggat tttggacatc tactcctagt
    16081 aagaaaatat tccctaatgt aacgatcaag ctttgcgcaa tcgccattgt caaaaatgtc
    16141 agcatacgag caacgaattg ttctcgtttc gtaaaattgt ttcggtcttt ctttcctaaa
    16201 tagtattctg tcgtagcaac acttgaaaga agaacagcgc cgacccacag acatagtgca
    16261 gtataaaacg gggtgctagc tgaaccgtta tttgcgattg gataaagcgc attggtttgt
    16321 agctcaacag gttgtgtaaa gaagtcactt tcttttgttg cgtctagttt caataatttg
    16381 atgacttcgc ctagatcgac ttcttgttcg ccttttcgga tcgcgtctgc tgctttttgg
    16441 ataccagctt tgatatttgg ccagtcattg ttgatcaagt ccgcagcagc gttcacagct
    16501 gtttcgactt ctggcatttt ttcatttact gttgtcaatg taccggtgat ttcatttttt
    16561 agtgctggcc aatcattttg gataaaatca gcggccaatc ctaatttttg ctgaatgatc
    16621 ggtaattcat tgttgtatag gtctgcccct tcattgatgc cattgacgat ggtatccata
    16681 tttccattca gcattgtatt ggcatcatgg atttcttgtc caattgcagg catttctttt
    16741 tgatattttt ctaaaattgc cactgcattc gttacagttg ctttcgttga gctgagaagc
    16801 gaatcaaagt cgatctggtc cgctttgctc agtgcgcctt gtgcagtccc aatcgtatcg
    16861 atgacttggt ctagtagaga agtgacagtg gaactgattt gacctgtatt gataccagca
    16921 atgatgccat tgatattatt aacaacggca tcaatttgat ctagcaattc atcaatagcc
    16981 ttcgtgtctt cattttgaac agctgtgttc aaagcagata ggcggttttg cagattgctt
    17041 agcaagggtt ttaattgatt tagctgatca ataattggct gtaatttcgt atttccagca
    17101 cttgtttgca atttagtcag gaactcgacc agtttatcga ttgcattttg ttgttgagat
    17161 attccgtcaa taaagccgtt caatacattg ctgatgtttt tcttttcatc atcagtcagc
    17221 ttaccatctt caatagctgt tcttacagta tctattaacg aacctgtcgt tgttgccact
    17281 tgtccaattg acttcaatgt aacatctaca ctgtttgtga tgcttggtat cgcatctttc
    17341 agctgagagg cacctgattt tgtttggttt gctagatcac ttgcctgatt ttctaaattt
    17401 ttcacatctg gcaagatatc ttgtacttgt tggatgatcg tcaagccttg ttttgcttca
    17461 tcgattcctt ggttcattgt ctcttggatc gaagcaaaat cgccgtcgat ctctgctagt
    17521 tgcttaccgg cattttggat ctctgggatt ttttcttgca aatcaaggat gatttttgct
    17581 tgatctttca actcaggcat tttgtcattc aatgcaacga ctttttcacc catctcatct
    17641 actttaggga tgtaatccac gaattcattt gctttattca gtttttcctt gatttcaggt
    17701 agttgacttt gtaattcgag gacttgtttt gtgtagccgt cgatcgtgtc caaattttca
    17761 tctgttgata aaatcatatc tttaacttta ttgatactta ctaggttcgt atcgatgtcg
    17821 taacctatct cattgaatac tttcaataaa gtcgaactgg ctgttttgat aaattcattt
    17881 gtgatttgcg attggatcga acttgctcct ttatcggtga ttttcggtgc aatcgcattg
    17941 attttttcat ttaccgtata ttcgattttt ggtttcttga tatcaccact agtaaaactc
    18001 agcagatctt ctgagaaatc ctttggcaag taaatacctg cgtaatactt tccggaacgg
    18061 actccgtctt ctaattgttc ttttgaatca acgaattgcc atcctaattg tttattttta
    18121 tgaagcgatt cgatgacttg ctccccaatg gccacttctt taccttgaaa ctctgctggc
    18181 ttgtctgcac tgtaaacagc aattggcagc tctccagtat ttccatatgg atcccataat
    18241 gctttgatgt taaaccacgc atatagcgaa gggatgatca tgatcgcgat gatcagaagt
    18301 gttgcaatcg ggtttttgaa aatccgtttc cagtcaagca aatataatcg aagcgtattt
    18361 ttgattgact gcatagttct cccccttctt cttttttcag ctgaattact ttaccatgta
    18421 tttcatactt ttttcaatcg gcttttttca aaaaagtcga atttttggac atatcacggc
    18481 aattgtctca ttgtaatctc ttacatattg agaaataatt aaggattaca gcctttactt
    18541 tatcatgaaa taaaagggaa agataatttt atgccaaagt ttttatctat tatacgtata
    18601 cgcaaaaaag ctaaaacatt ttttgtcttt gcccctacaa aaatgtttca gcttttttcg
    18661 ttttttattc ctgtatttct tatttttcga atgacaggat gattttccca gcaggaggat
    18721 gtccttcaac tagctgatgt gcttggatga catgcttaag tgaagcagac agattcattg
    18781 aaataaaaac atgaaaggct cctttttggt aggctttgat tatgccagca aaggcttctg
    18841 catctagata ttcctttctt ggaacaaacg attcataaaa gccttccttt ttttgccgca
    18901 aatctagatc tgggaggtca tttaatgcaa catacctgcc gcctggtttc atgatctgga
    18961 tgccggtttc tcctttgata cttcctttcg tcgcatcgat tacaaggtct gcctgatcag
    19021 caaattgcag acctggattt gttttatcat aagcagcaaa tgctgaagct cctaattttt
    19081 ttaccttttc ctcattttta ctggatgcac tggttaagat tcggattccc ttttcatgga
    19141 gaagctggat cagacttgac ccgaccgcac ctgatgctcc tagtatcatt acagtatctg
    19201 tcggctggat ttccgtcaaa tggttgatta gattataagc cgtaattcct ggcgtgacca
    19261 ttccggctgc ttcttcccaa ctcattttat ctggaatttt tgctactttt gctgatggca
    19321 acacgacttc ttctccgtaa gtcccgccga ctgcatggac agctactcga tcacctacat
    19381 gaacagttgt gacatctgaa ccaatctctg tcacaatacc agcgccatca ttacctggta
    19441 cataagggaa tttcaatgtt cgtatctctt tcatttctcc taaacgcaag gcaacatcat
    19501 aaggattcac actgaatgct tttatttcta cacgaacatg tccagaaccg acttctctgg
    19561 gatgagcatc gatttcttca aaaacatctt gtgcttctcc gtaaccgtta attgcaaatc
    19621 ttcgcatggt caccatctcc ctttttcttt aagtttacgt ttatgccttt attttgacaa
    19681 aaaaaatgtc ttttcgtttt ggcgcatgat tcaaaacaaa atagttgtct tttttcgcgt
    19741 aacgtgttaa attatttatc taaaagcgta aagattacga tttacgctaa taaaaaataa
    19801 ttggaggaat ttgttgatgg ctaaaaaatc aaagattgcc aaagcaaaaa aacagcaaga
    19861 aacgattgaa cgctatgctg ctttacgcca ggaattaaaa gcgcaaaaag attacgaagg
    19921 cttgcgtaaa cttcctttag atgctcgtcc agaacgattg aaaaatcgtg atttaatcga
    19981 tggacgccct cgcggataca tgcgtaaatt cgggatgtcc cgtataaaat tccgtgaatt
    20041 agcacataaa ggtttgattc caggagtaaa aaaagccagc tggtaatcca agtctggctg
    20101 taatgtaaag gaggagggcg catggaccac tccttggatc atcaaaaaag atacgttcgc
    20161 ttcttctagc agaaatgctg gtactagaag cagacgtatc tttttttacg cacagctggt
    20221 ccttttttat atgtaaaggc tgcatggtta cttcgctata tttatttagt aacaggagtc
    20281 agtgcttcga tacccacact aattttcaat gctcggtcaa cttctgccat aatgggtcga
    20341 tccaaatggc agaccttttc tttcaatctt gatttatcga tcgttctgat ctgctccaac
    20401 aggataacgg agtctctttc tattcctgtt ccagtagctt tgatcccaat gtgagtcgga
    20461 agcttaggtt tagccatttt tgccgttatg gccgcaacaa tgatcgtcgg gctaaaatgg
    20521 ttacctagat tattttgaat aaccaaaact gggcgtgttc cgccttgctc cgatcccacg
    20581 actggagaaa gatcagcaaa ataaatatct cctcttttta ccataggatc ctccttattc
    20641 ttgatattct cttggtatcc gctgtcctaa tccgcaagcc acttcatagt ggatcgtacc
    20701 taattgatct gctatatcct gcatagtgat ttctttgtct gcatttttgc cgataagcgt
    20761 cacttttgtt ccgacaggaa attcttgagg caatcggatc atcagttgat ccatacatac
    20821 acgcccaatc gtttcacagt agttcccttc taccaataga gaaaaaccct gcatctttcg
    20881 tggccagcca tctgcataac cgatcggtat cgttccgatc cactctgctt caggagtgat
    20941 atatgtttct ccatagccga ttccttcacc tgctgggagt tttttgactt gaatcaattc
    21001 agaaaccagc tcaagtgctg gctgcaatgg ataaacttcc ggtagtgcgt gcccagaggg
    21061 attaagtccg tacattgcta ctccataacg aatcatattg ccgattgttt catcatgcca
    21121 taaagccgtt gcactattgc tgacatgtac atatcgagga agttcgggaa gttttttcag
    21181 cacttctata aagcgttctt tttgcagatt ccaatatgta tcatctgcct gatctgctgt
    21241 agaaaaatgg gtaaataaac cttcccattc taatacacga ctttctttga tgaatgcagc
    21301 tgcctctttt acttcttcag gtgtacaaaa tccgatacga cccatcccag tgtctgcttt
    21361 tagatgaatc gaaagaggcg tctctagctt acttttctct aaaacagcag ctgctgcttg
    21421 caaccattcc cgtgtacctg cagttactga taaatcatga gcaacagcta cagtcagtgc
    21481 gtcgtaagga accatattca agataaggat gggctgagta aatccagctt ctctcaactc
    21541 gactccttca tctaaatttg aaacacaaaa gcccgaagct ccacctgcaa ctgctgcctc
    21601 agctgtttca atagctccat gaccgtatcc gttcgctttt actactgcaa acaattcttt
    21661 tccttgcgga agtcttgcaa cttcatttgc tacattttca gttatagctt ttttatgtat
    21721 cactgctttc gttgggcggt gccaagcgac aaccatcgtt tttccttctt tctagtcttc
    21781 caaaataatt tgtgcaaaag ctgtttcatt tgtatgagta atactgaccc atacattgcc
    21841 ttcatgaggt gaacgcgtca caacaggctg accattttca ttttttaaaa tctcgacatc
    21901 ttgaaaggta acttttccga tcccagttcc ccaagcttta gagaaagcct ctttgcaagc
    21961 gtaacgcccg cctaaaaatt ctacttggcg atgaaaaggc aaggactgga atagtttcat
    22021 ttcatcagag gtcaagattc gtgcaatgaa ttttggtttt tcttcgatca gccgagtgat
    22081 cctaggaagt tcgactgcat cgatcccaat tccttttatc atatgctttt ctcttttcaa
    22141 taaatttaga acactctcat tctaccaaat tataaccatt ttgccacaaa taagtccaca
    22201 aataacaaaa atgaaagttt ctcttaaaaa agagaaactt tcattttatg tttaatcgtt
    22261 atttgaacgg attacaaatc cgcgtttttt atcattgtga cgtttaccgc catctttttg
    22321 gctgttatta tttttgctat aaccgccttt tgatttgttt ccacgataat taccgccgtc
    22381 acgacgatta cggttgtttc cgccaccacg tttgccattt ttattgtatc cttttttctg
    22441 cattggcaat gggcgttctg gtgtgatctt cactggcact gcatcagctg gatcttttgc
    22501 tgtcgttttt agcaaaagtg caactaaatc ttgtgcagaa tattcttcta ataatttatc
    22561 tgctgtttgc aggtatttat ctagaccatt ttctgcaagt tttgtttcga tttgttcaac
    22621 tgcagcacct aattgacctt tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat
    22681 ccgttttttc gtcaagtttt caatcacatg caagtagtcc atttcatttg gtgtaacgaa
    22741 agtcacagac ataccgcctt ttccggcacg gcctgtacgt ccgatacggt gaacgtagct
    22801 ttctggatct tgagggatat catagttgta cacatgagtg acacctgaaa tatctaatcc
    22861 acgtgcagca acgtctgtcg ctacaaggat atctaaatga ccgctcttaa atgaacgcaa
    22921 tacgctcatt cgtttttgtt gagacaaatc gccatggatt ccttctgcac ggtaaccgcg
    22981 tgcttctaat ccgcgtgcca attcgtctac acggcgtttt gtacgtccga agacgattgt
    23041 taattcaggt gtttgaacat caaacaagcg agtcatgata tcgaattttt cgtattcttt
    23101 tgcgcggaca tagtattgat cgatcaagtc agctgtcatt tctttcgctt tgatcttcac
    23161 atgatgtgga tttttcatga atttaacgcc aatattcttg atagctggcg gcatcgttgc
    23221 tgagaaaagt aatgtttgtc tttgatcagg aacttgtgaa atgatttttt cgatatcttc
    23281 caagaagccc atattcaaca tttcatctgc ttcatccaaa actaatgttt gaacggttcc
    23341 aagtttcaat gtgtggcggt taatgtggtc aagcatacgt cctggtgtac ccacaacgat
    23401 atgtggacga tctttcaagc cacggatctg acggccaata tctgctccgc cgtaaacggc
    23461 ttgtacacgt acttttttgt cgcgccctaa acgatataat tcttcttgtg tttggatagc
    23521 taattcacgt gttggtgcga taactaaacc ttgtaattcg tgacgatctg gatcgatttt
    23581 ttctaacatt ggtaatccaa atgcagctgt tttccctgta cctgtctgtg cttgtccaat
    23641 aacgtctttt ccagctaatg ctagtggaat cgtttctgct tggataggcg ttgcttcttc
    23701 aaaaccagcg cgttcgactg atttcaacaa ttctggtgat aattctagtt ctttaaattt
    23761 caaatgaatc ctcctatatg gttgttaact gaggatgtag cttttttgct accccgtcga
    23821 tttgttcggt caaagtgtat tgtttctttt tgacagtggc cacgcacgtt tttatccatt
    23881 cttagcccct gtggcaccac cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca
    23941 aacgggtcgc gttcagcttt atcattatta tgacatctgg cttcaacaag ttgtttctat
    24001 ggcaaaacca gtccaatttc tcttcatctt tcggcaatag gttattctaa catagttttt
    24061 cgttttctgc aagtttacag atttttattt catttgctga agttgttcta ccacttcaaa
    24121 taagcccatg ctgttgctgc cttttagcat aatactatct gttgcttcaa gattttgttt
    24181 gatagcatca atcaatttat tcttttcttt ttcaaaatag aacacaggta gttgggggta
    24241 tttttggttc aattcttctt ttagtgcatg catttgttcc ccataaagga aaatcatggc
    24301 gaatttctca ggatcgatat gttcactcat ttgcgcatgc atatttaaag actctttccc
    24361 taattccagc atatctgcta acacagcgat tctgcgtcct tttgttggca gatttttaaa
    24421 agtatctaat accagtccca tggctgttgg atttgcattg tagacatcgc tcaaaatagc
    24481 tgcaccattt cctgccgtta gccattcggt tctgttttga gtgatagaaa catgggcgag
    24541 cccttggcgt atctcaggta tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa
    24601 agcattcgtt acattataac ttccaggcag cgggatcgta aatgtttctc cttctactac
    24661 aaaatcggtt tgtgcttttc cttttgctgt cacttccgca tgaacatcgc catttccaat
    24721 accgaatgtt gtgactgttt gtactaatgt tgccgttaaa ggtgtcagca acggttcatc
    24781 tgcgggcaca acgagtaatc cgtctgatac aagtccgtca gtgatttcca ttttggcttt
    24841 agcgattcct tctcgcgacc caagattttc tatgtgtgct tcaccgatca ttgtgattgc
    24901 tgcagctttt ggttgagcca taagtgaaag ttccgtgatt tcattggcgt gatccatgcc
    24961 catttccaaa acgatctttt ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc
    25021 aatattattg ttgtaatttc cttgcgtctt atatgttttg tatttttcag acaagacaga
    25081 cgcggttaga tctttcgtgg tggttttacc attgcttcct gtaattgcaa cgacttctgc
    25141 gcctactttt tccaagtaat aggttgccaa ttgctgcatg gctttcaaag gatctgctac
    25201 aggaataact ggaaaatttt ttggagcttg ttcaacaggc aagctccaaa atgaagcaga
    25261 tgctccgttt gtgatcgctt gctggataaa ttcatgtcca tctcgcgtac cagcaagagg
    25321 aacgaataaa cttccttcgg ttatcttccg gctgtcaaat tcgacattgg taatctttgt
    25381 cagtgcagag cattcgctgc ccacggcttg tgcaatttct tgtattgtta gattcattga
    25441 gatctctcct aaaaaaggtt gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac
    25501 tctcacttcc aagtaaattt ctctattcgt atttagaagc tagaatgaac gcggttacgc
    25561 aatcactcca gcctcttctt tatttctctt tattcatttt tttcaaaaaa agattgacgc
    25621 tgatggtatc gattcattcc taactggatc agttcttcaa tcaaatcacc gtatttcaag
    25681 cccatatttt cccataagag tgggtacata ctgaactccg taaatcctgg catagagttt
    25741 aattcattca ggaataattc atttttattt gtcaaaaaga aatcgcaccg gctcaatccg
    25801 cttccaccta acatcgtgta agctaacttc gcgtactctt gcgctttttg ataaacttct
    25861 tctggcactt cggctggaat ctgcatttcg attttattat tgatatattt tgcttcataa
    25921 tcatagaatg ctacgtcttt tacgacttca ccaggcaaag tcgtccgaac gtcttcattt
    25981 cctaatacag caacttcgat ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa
    26041 tcatactgat aggctgttgc taaagcattt tgcagctctt ctcggttttc tgcttttgta
    26101 atgccgacac tagaacccat attcgccggt ttgacaaaca tcggataaag caaagaacct
    26161 tcacattgat caaatacttt tttaggattt tctttccatt gattcttaag tactggtaca
    26221 taaggaactt gcggcacacc agcagcttgt aaaatatact tggtcatgat tttatccatg
    26281 gcacatgcac tggtcaatac gcctgcgccg acataaggca tattcaatgt ctctaagaag
    26341 ccttggatcg ttccatcttc cccgtttggc ccatgtaaaa ctggaaaaac gatggctcct
    26401 tcttctttga tttcgcccgg attgatcact tttcctgtaa agccttcctc tgtctgtcca
    26461 cttgggtccc atgaaagatg caagacatct ttgctggcag gtttttctgt tagtaatgga
    26521 cctttgaccc attgtccttc ttttgtaata aatacgagtt gaacttggta ataattataa
    26581 taaatggcat ttaaaactga aaatgcggaa agaatggaca cttcatgctc tgcgctgcgt
    26641 ccgccatata gtaaagtaat cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg
    26701 atttactctc atattttagc acaaaacaaa aataaacgaa taacttattt ataaaaaaac
    26761 gagccagctt ctaaaagaac gagcgtcatt tattttttgt cagctagatt atttgtcccg
    26821 ttttttataa aaagttatga taaagaaaag gagggatgat gatggcgcaa acaaatccac
    26881 gcagccgctt ggcagaaatc attcgagtgt tcatcaatta caatgtagtg ccaaactttg
    26941 tacagcaaaa aaatcccgaa caggtaaaaa aagcctttga agaactaggc cccaccttca
    27001 tcaagatagg acagatgctt tctgttcgag aagatttgct ttcttctgcc ttcactcaaa
    27061 cattcaaaac attacaagat agtgtaccta gcgacacatt ttcaacggtc aaaaagacga
    27121 tcgaaacaga actttctctt tcattatcag atatattcga tgatttttca aaatcgcctt
    27181 ttgcctctgc ttctatgggg caggctcatc gtgccaagct gaagaacggt gacgcggtag
    27241 tcgttaagat ccaacaccct aacatcgccg aagagatccg acttgatctt caactatttg
    27301 aacgagcgat ccctttgatc aaatacattc ctgaaactag cgtagttgat ctaaaagggg
    27361 tactccaaga agtaaaacgt tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa
    27421 atggcgaaca attctatcaa aagaacaatg gctggaaaga gatccgcagc cccaagattt
    27481 atgacgcttt ttgttcgaaa aaagtgatcg tgatggaaga aatgtctggt aaaaacttga
    27541 atcatttaat gaacgccgaa aacaaaactg aaacttttat cactggaatt caaaacaagc
    27601 agctgaaaca agaagttgca aagcttttag ttgagaactt tatgaaacag gtgtttgatg
    27661 atggtttttt ccatgctgat cctcatccag ggaatctgct attccatgta ctaacaaaag
    27721 aagaacagac acaagctagc cgaaaaactg aaacagtcca tgaaaaagag ttcggttcct
    27781 ttgcttttcg agcctccact tctgcagaag accctgttgc gccatatacc atcaactaca
    27841 tcgattttgg tatgatgggg catctttctg caggacttag gcaaaaactc acacaagccg
    27901 ttttagcgct gtatacgaaa gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc
    27961 agcaagaagg ctcgtttgat gaaagtcggt tccaccaaga actgacaagt tttcttgaac
    28021 agtattatga ttctcctatt gatgaaatca acttgcaaga agtattcaca catgtcgtga
    28081 cgatttgtca tcaaaataat ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat
    28141 ttgggacatt agaaggggtg atccgcgtcc tcgatcctga agtttccctc atggaagtcg
    28201 cttcgccttt tgctcaacat tattttctca ctcatctgga tgtagaagat acattgaaac
    28261 aaagcggatt agacttattg gaagggatga aggctgcgcc taagatccct caacagctcc
    28321 accatctatt agaaatgtgg acttcgggac aaggaaaagt caatttggaa ttgaaaaagc
    28381 aagacaaatt attgagcagg atcgaaagta tgatcaatcg gcttgttttc ggtatgattt
    28441 tagctgcatt gatcgtaggg tcctccctgc ttgtccaagc agcacctgta gaaaacgcag
    28501 aggtcgtttc cttattaggg atatttacgt atgctattgc cgcattcgtc attatttttt
    28561 tggcaatcga tgcattgatc caaatgtata agaagaggaa aaagtagaaa aaggttgtga
    28621 cagaaagaat cagcttcaag aaataagaag gggttttaca taaattgttt ctcaaatttt
    28681 tgtgaaaatt cggcttattt ttcgaagagg ctacttctga aacaccgaga ataaaaaatg
    28741 tgaaaaagct gttctgcacc cagtaataag aacaatcaaa aagggaacat ggaacacatt
    28801 tatcctgttt ccctgttccc tttttgatgt attcgagcat aaataaaata ccttcgttac
    28861 ctactatctt ctgtacttgg taaaccttag tcaattgcct tttttaacgc ttgacgaaaa
    28921 gacatatgat ctttctccgt aaagggtttt ggaccctttg tccttatgcc agcttttctt
    28981 aactttccac tatgataacg ttgttctaga agcgtgccga tattcgcttc gtcaaattct
    29041 tttccagagt gatggaacac tcgcacaccc ttcggcaata caagtgaagc taaaccataa
    29101 tcttgcgtga tcaaaaagtc attcttctta accataccga cgattttata atccgcacta
    29161 tcagctccgc ggtctacgta gacgatttgg acatgattag cgtattcttt ggtcgtataa
    29221 tggtccacac tggtcacgat aaccacgtca agaccatagg attctcctaa ttttatgaca
    29281 tcttctttta ctggagaacc atctccatca atgaatagcc tcattctttt tcgccttctt
    29341 ccataaatga taaacagtta ctaatccgct cagtaaaaga ccaacattga tccattttgc
    29401 caagataagc agttgtacac tttctatcgg cactgattca tggttcagcg tgatcaataa
    29461 tagatggatt ggctctccca tttcttttgc taaaacaaac aaaagcgtca tcaataaaaa
    29521 ctggtggatc acactccctt tattttgagt agtatacttg acaacgatcc ggcgatccat
    29581 tggaatagct gtatcagttt gataatattc cttatttagc cagctgcctg ttactacgtg
    29641 ccagaaacta agcagaaaga cacctatcgc aaaaccattt ggaatcaaga ggaccaataa
    29701 agaagaactc agtccaagca attgcatagc caatggagac catgttaaac gtttcaaaat
    29761 ccatggtccg actatttttg ctgcaatcaa gcctgctgca taaggcaaat acataaaaat
    29821 cgaaagtttt tctaatccgt acagtatgcc aacataaaaa ctgctaaata aaaaaaggaa
    29881 attccctagc attccattta gaaaagtaaa cagatttagc cagcttggca acttccattc
    29941 cttcttctgg tgtcctaaaa caaacacgaa caagatgaac agcagcaaga aaccataaat
    30001 agcaagatca aaaagccttt catcgaataa caatcgggca tttctcaaaa gaaacaataa
    30061 gccaaagaat atcaaaaata aaacaagctc tcgcgtagct accagatttc ttttgatgtc
    30121 tttgaaatcc agttcgtaac gcggataatg actaaccgta tggtaggcca tgacgtaaaa
    30181 aagtgtataa atagtaaagc ttgctgcaat ctgagtcggt gcaggcaact caatcgcttg
    30241 atacaggaga aatagccaga tagctgcaaa taagtacttt ttcccatcca tattgataaa
    30301 accttgcgtc ttttcgtgaa aattgaccgt ggtattagca ggtacaagcc atgctgcact
    30361 cagccctagc aaaacacttg aaaaataaaa taaaggaaaa taaaatacgc ctaatattcc
    30421 aagcaatgaa ccagcaccac caatcagcag agcgatcatc agcaacgtgt aactatctag
    30481 accaaatcgt agactgttga ctaaaaatag tccagtcatc cgaaaggcat aaaacagcgt
    30541 aaatggcaaa atcgtcagcc aattatgcga gtctgttagt cccaaaaata aaagataagg
    30601 aataaaaata accgtgttac ttaagataaa gggcgttgtt tgtgtaaaat tacgcccaaa
    30661 tcgtttgagt ttttcgaaca tgacaatggc tccttaaaca gtcttataac taatctgctc
    30721 ttcgatttta gcattaaagg ggaaataaac caattttaca gaatatattc ttgtaaaaaa
    30781 aaatacagaa agaagcataa gcttctttct gcatttttac ggtacttgat tactcaagca
    30841 gacaagccct tctccacaaa agtcttgccc gacacccctg catccggcag gtgataaaga
    30901 tagaaatcaa ttatcctgtg cctccaaaat caattaggag ctctgtaaac cagagtcctt
    30961 ctcgacccgt cgcataaatt tactttaaca cagttcctat gtttatgcga gagctttttt
    31021 cattttataa tcgaccattt gaaatccttg accgatatct gaaataagtt cttctgtaat
    31081 ctcaaatcct tcatgcttat aaacctcgat tgcttgatga ttcccctgat tcactcgcaa
    31141 aaagatctct ctttcctttg tttgcgcagc aagttcttca taccagcgaa aaactgaaga
    31201 agtcaatcct tgaccacgta ctttatttag caaatataat ttacttaaat aaatttttcc
    31261 atttatctct tcataagcag tgtaaccgac agtcttttct tcgcttttta ataaaaagta
    31321 ggacacacct tctgtcagtt ctttttgaat ctgttttttt gactgataag ttttcagcat
    31381 gtaatccact tgtttttgac caatgatcgg cacaaaaact tccggccata cttcttgtat
    31441 gactgccatc agctcatcca gttcattttc tgttactttt attttttcca tacacccacg
    31501 tcctttttta cattataaat gaccttgcgc ttgatggaat aaaaagagca tgatcatgct
    31561 atacacaccc accgaccacg gtttcaagaa aatcacaatc tgcataaatt ccctaaaaga
    31621 taatttcgtc aaaccagcta ctagacagat caagtcgtct ggcgcaaagg gaagcagcaa
    31681 ggtaacaact agtacttttt taatatcttt cgtttttctt gtcagcaatt catcaaattt
    31741 ctgatacttt tttggcgata aaataagctg gacaaaggct acgccatagt aacggacaag
    31801 caaaaatccg attgcttccc caatcactag gccaatacaa ctatataaaa gcccaaatcc
    31861 attgccaaac atcagcatgc ccgctactga ggagatccct cccggcaaaa taggaatgat
    31921 cacttggatc acttgcaaca gaacaaatat catcactgca tacttgccaa attgctggat
    31981 aaacgcttgc aaagacgtgc tcgatcgaaa aatcccatga tgataagcat aaagaatcaa
    32041 cacaaagaaa aaaaacagac caataatggg catgatctga agacattttt gtaaaactga
    32101 tcctttttta gacatctata ttccccacat cttttttcat ttattaaaac tgcctcct
//
LOCUS       DACDTH010000029        31688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDTH010000029 DACDTH010000000
VERSION     DACDTH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.029"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(99..341)
                     /locus_tag="IWK54_001414"
     CDS             complement(99..341)
                     /locus_tag="IWK54_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9593540.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(373..930)
                     /locus_tag="IWK54_001415"
     CDS             complement(373..930)
                     /locus_tag="IWK54_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9593541.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(943..1131)
                     /locus_tag="IWK54_001416"
     CDS             complement(943..1131)
                     /locus_tag="IWK54_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9593542.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(1144..1710)
                     /gene="amaP"
                     /locus_tag="IWK54_001417"
     CDS             complement(1144..1710)
                     /gene="amaP"
                     /locus_tag="IWK54_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9593543.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            2940..3413
                     /locus_tag="IWK54_001418"
     CDS             2940..3413
                     /locus_tag="IWK54_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9593544.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            3422..3649
                     /locus_tag="IWK54_001419"
     CDS             3422..3649
                     /locus_tag="IWK54_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9593545.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            4285..4656
                     /locus_tag="IWK54_001420"
     CDS             4285..4656
                     /locus_tag="IWK54_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593546.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(4912..5154)
                     /locus_tag="IWK54_001421"
     CDS             complement(4912..5154)
                     /locus_tag="IWK54_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9593547.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(5186..6088)
                     /locus_tag="IWK54_001422"
     CDS             complement(5186..6088)
                     /locus_tag="IWK54_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9593548.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(6101..6289)
                     /locus_tag="IWK54_001423"
     CDS             complement(6101..6289)
                     /locus_tag="IWK54_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9593549.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(6303..6866)
                     /gene="amaP"
                     /locus_tag="IWK54_001424"
     CDS             complement(6303..6866)
                     /gene="amaP"
                     /locus_tag="IWK54_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9593550.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(6904..7797)
                     /locus_tag="IWK54_001425"
     CDS             complement(6904..7797)
                     /locus_tag="IWK54_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9593551.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(7875..8813)
                     /locus_tag="IWK54_001426"
     CDS             complement(7875..8813)
                     /locus_tag="IWK54_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9593552.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(8847..9197)
                     /locus_tag="IWK54_001427"
     CDS             complement(8847..9197)
                     /locus_tag="IWK54_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9593553.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(9230..10132)
                     /locus_tag="IWK54_001428"
     CDS             complement(9230..10132)
                     /locus_tag="IWK54_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9593554.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(10125..10982)
                     /locus_tag="IWK54_001429"
     CDS             complement(10125..10982)
                     /locus_tag="IWK54_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9593555.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            11316..12125
                     /locus_tag="IWK54_001430"
     CDS             11316..12125
                     /locus_tag="IWK54_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9593556.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            12165..12662
                     /locus_tag="IWK54_001431"
     CDS             12165..12662
                     /locus_tag="IWK54_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9593557.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(13047..13120)
                     /locus_tag="IWK54_001432"
     tRNA            complement(13047..13120)
                     /locus_tag="IWK54_001432"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13084..13086),aa:Ile,seq:cat)
     gene            13307..13630
                     /locus_tag="IWK54_001433"
     CDS             13307..13630
                     /locus_tag="IWK54_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593558.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            13794..14048
                     /locus_tag="IWK54_001434"
     CDS             13794..14048
                     /locus_tag="IWK54_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593559.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            14118..14363
                     /locus_tag="IWK54_001435"
     CDS             14118..14363
                     /locus_tag="IWK54_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9593560.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            14439..14804
                     /locus_tag="IWK54_001436"
     CDS             14439..14804
                     /locus_tag="IWK54_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593561.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(14865..15428)
                     /locus_tag="IWK54_001437"
     CDS             complement(14865..15428)
                     /locus_tag="IWK54_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593562.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(15996..16196)
                     /locus_tag="IWK54_001438"
     CDS             complement(15996..16196)
                     /locus_tag="IWK54_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9593563.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            complement(16948..17661)
                     /locus_tag="IWK54_001439"
     CDS             complement(16948..17661)
                     /locus_tag="IWK54_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9593564.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(17654..18739)
                     /gene="uxuA"
                     /locus_tag="IWK54_001440"
     CDS             complement(17654..18739)
                     /gene="uxuA"
                     /locus_tag="IWK54_001440"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9593565.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            complement(18756..19199)
                     /locus_tag="IWK54_001441"
     CDS             complement(18756..19199)
                     /locus_tag="IWK54_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9593566.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            complement(19233..19586)
                     /locus_tag="IWK54_001442"
     CDS             complement(19233..19586)
                     /locus_tag="IWK54_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593567.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            complement(19698..20099)
                     /locus_tag="IWK54_001443"
     CDS             complement(19698..20099)
                     /locus_tag="IWK54_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593568.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            complement(20136..20645)
                     /locus_tag="IWK54_001444"
     CDS             complement(20136..20645)
                     /locus_tag="IWK54_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9593569.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            complement(20667..21524)
                     /locus_tag="IWK54_001445"
     CDS             complement(20667..21524)
                     /locus_tag="IWK54_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9593570.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            complement(21542..22375)
                     /locus_tag="IWK54_001446"
     CDS             complement(21542..22375)
                     /locus_tag="IWK54_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9593571.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(22389..23186)
                     /locus_tag="IWK54_001447"
     CDS             complement(22389..23186)
                     /locus_tag="IWK54_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9593572.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(23219..23503)
                     /locus_tag="IWK54_001448"
     CDS             complement(23219..23503)
                     /locus_tag="IWK54_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9593573.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(23500..24501)
                     /locus_tag="IWK54_001449"
     CDS             complement(23500..24501)
                     /locus_tag="IWK54_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9593574.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(24503..25405)
                     /gene="gnd"
                     /locus_tag="IWK54_001450"
     CDS             complement(24503..25405)
                     /gene="gnd"
                     /locus_tag="IWK54_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9593575.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            25568..26416
                     /locus_tag="IWK54_001451"
     CDS             25568..26416
                     /locus_tag="IWK54_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9593576.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            27062..27268
                     /locus_tag="IWK54_001452"
     CDS             27062..27268
                     /locus_tag="IWK54_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9593577.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(27470..28471)
                     /locus_tag="IWK54_001453"
     CDS             complement(27470..28471)
                     /locus_tag="IWK54_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9593578.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(28476..30389)
                     /locus_tag="IWK54_001454"
     CDS             complement(28476..30389)
                     /locus_tag="IWK54_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9593579.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(30557..31063)
                     /locus_tag="IWK54_001455"
     CDS             complement(30557..31063)
                     /locus_tag="IWK54_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9593580.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            31223..31663
                     /locus_tag="IWK54_001456"
     CDS             31223..31663
                     /locus_tag="IWK54_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9593581.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
BASE COUNT         9152 a         6310 c         5071 g        11155 t
ORIGIN      
        1 aatacttcat ataaaatata taaaaaagaa ccgcagcgag tgtcgctacg gttctcaaat
       61 ttgttcttca tttcaacata agtttttctt caatttgctt ataatttacg tgcaaagaat
      121 gaaacgacta atactaaaat gattgcaccg atcaatgatg gaatgatagc cattcctgca
      181 attgttggtc cccaagttcc tagtaaagct tgtccaattg ctgaacctac taaaccagca
      241 atgatgtttg cgatgatccc cattgatgat cctttattcg tgattgctcc tgcaattgct
      301 ccaatgatac cgccgacgat taatgcccaa ataaatccca ttttaaattc ctcctaactg
      361 acttcttact tcttattgta cacgtggttc gttattttct ttgacttttt gtgcaccttt
      421 gcctacagca gattttgctt tatctgtttg gcgtgaagca aattgtcctg tggattttgc
      481 tgcttctgtt actttgtctt gtactgtttc ttgatctttt tggtattctt cttgtgtttt
      541 gatgtctgca acattcacat tcacttcgat aacatctaaa tgtgtcatat tgtttacttc
      601 agtactgatc aattctttga tttggttgta aatatcttcg atgtctttac cgtattctac
      661 gatcacatct agatccacag ctacttgttt cttacctact tctgtattga tacctgctgt
      721 tacgttatct gtgttgacta atttttcagc catatttgag aaaaatccgc catctaatgt
      781 caatagacca tctacatctt tcaatgcata accgatgatt ttttggatga ctttgtcttc
      841 ataagtcaat tcaccattca tcatgcggtt ttctcctttt tcatttgcca aaactgatcc
      901 atttgttgta ttcatgtttt tgttttccat ctttgtttcc tcctatgatt taaaaaattg
      961 atcaactatt cctgactgtt tcaaataaaa accgattcct gcgccaattg ctgtcatgat
     1021 tatgatcaac aatgtcttga aaaatccaat cgttaaccat aaaacagcta gcagtagtcc
     1081 aagaccccca aagataatag ggagcttgta tgctgaaaat agttctttca ttatttggcc
     1141 ctcctattcc actcgagagt gcttgtagtc tcttgtatct tcttgttctt cataaccgct
     1201 gtatgtgact gccactttga ccgtttcttt tgttccgagt actttagtta cttcttcttc
     1261 gatatcttgc atcaatacac ctgttttccc tactaatgaa gaagttcgtt tcaagtctcc
     1321 gttgacatgg atatggattc gatttttcgt tgaacgtatc cgtactttag gtgaatgaat
     1381 gaactcttct tcgtgtaaat gggaacgtac caacccttcg atcgcttttt tgtcgattgt
     1441 tagtttgcca tcttcccgtt tcataacgaa agttcctctt gactttggat agaaaagaac
     1501 gactagcaag atgataatag caagaacggc aaatactaca gctgcccaga aaatatattg
     1561 ttgcatgtag taattcgtta aagtaaagaa gcgaaatcgt tccaattgtt caggcataat
     1621 atacggcgca ttgatcaacg caatcgctag aaatactgaa agtagcagta aactcatgat
     1681 aacaccgatt atttttaacc ccttattcat tttccacctc ctataaactt atttttaccc
     1741 tgtaaataac tctaccgaca atcacagtat agatagcgaa cagttagcat agcatcagta
     1801 aataaagcgc taccaaacat tgctggatca taacggcgtt ttattgccag tctgatactg
     1861 aaataacaat tgcttactta ttctctattt acttttgatt gctggctata cttaaaggat
     1921 attattcaag attaaaaatt gcaaagatta tgctcagtta ttattacaag attctattta
     1981 tttattaaat tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc
     2041 gttcttactt tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc
     2101 ttataaataa aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa
     2161 cgattggcta tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat
     2221 tcagttggac tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac
     2281 aaaattatcg aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta
     2341 tggtactttt tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac
     2401 ttttgattta taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga
     2461 tagtaccacc ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata
     2521 aaggacaaat aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa
     2581 taaacatgaa cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct
     2641 catacagttc tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta
     2701 ttgtccaata taatcccagg catctaaact agcattatag aagagacaca catagaatct
     2761 aaaataactg gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac
     2821 cttcatactc tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag
     2881 ttgttttaca ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa
     2941 tgaaatttaa aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt
     3001 tgttcggcgt acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt
     3061 ggcagaccac attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg
     3121 cttatttctt attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa
     3181 gcttaaacat actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg
     3241 gcttacttcc taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg
     3301 taggaatcgc tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa
     3361 aactgcttga acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga
     3421 catggcaatt atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga
     3481 attgagtgag aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc
     3541 aaaagccatc cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg
     3601 tgatttgttg acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga
     3661 acaaacagta aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt
     3721 acgtctatta ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat
     3781 gtactgactc atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca
     3841 actggtttcc tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca
     3901 ccagactgat agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt
     3961 tcataccttg aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat
     4021 tcttttttag taagctccca tatatactgt aaagccttct atatattatg tcatgttgct
     4081 ttttaacatt tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct
     4141 tttttatttt taacattatt ttgataataa tgattattta tttaaaaata cattagataa
     4201 catttgttac gacacgaaaa agcttcacaa acaaatatcg ttattttaaa atgagggcga
     4261 actcacacaa ttaggaggaa tagtatgcca tttattgttt acttttttct ttcattactc
     4321 gtgatctata ttccattacc aacatttttc ctgctcgtca atcgtctagc agagcaacat
     4381 gatacgattg ctgctcttac gaaactttgt ttacttctat ttgtttgtct agattacttt
     4441 acgagctatt attggctaaa acagacaaga gtaaaaaaga gatattattt cttccttgtc
     4501 tttttcaatc tctttgagtt tctgatccat tgctttttac ttcatcagta tcatacgatc
     4561 catttgtgga tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt cacctttcct
     4621 atctcaagaa aatttaggga ggcattgttt tattaaagaa gcttctctgt atcaataaag
     4681 acacatttaa ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat aaataagagg
     4741 ctgtgacaaa agtcttgtca cagcctctta ttccgtataa acagtgttca aaagcttgca
     4801 tcttcgataa tatgcccaac caaatgaaaa atatgagaag ctatttttgt ttggctgttc
     4861 ttattacttg atgaagaaca gctttttcac gacctctttt taaaagtctt tttacagttt
     4921 tcgagcaaag aatgatacga ttagtactaa aatgatggca ccaatcaatg atggcacgat
     4981 tgccattcct gcaatacttg gtccccaaga acctaataat gcttgaccaa gtgctgatcc
     5041 aactaaacct gctacgacat ttgcaataat ccccatcgaa gaacctttat tcgtgattgc
     5101 tcctgcaatt gctccgataa ttcctccgac gataagtgac caaataaatc ccatgtttcg
     5161 ttcctcctaa tgttttactt ccttattact gaacccgtgg ttctgtattt tcttttactt
     5221 tttgtgcgcc gcttccagct gctgatttta ctttatctgt ttgattcgaa gcaaattcac
     5281 cggttttttt cgctgcgtca gttactttgt cttgcaccgt ttcttgatct ttttcaaatt
     5341 cttcttttgt tttgatgtcc actacgttag cgttgacttc gatcacttct aaatgtgtca
     5401 tattttttac ttctttgccg attacttctt gcatttcttc aaagattttt tcgatatctt
     5461 tgccatattc cacaatgaca tccatatcaa ctgctacttg ttttttaccg acttctgttt
     5521 cgatcccagc cgttacatta tctgtattca caagttttcc tgccacatta gagaagaaac
     5581 caccgtttgc agataataaa ccgtctactt gttcaattgc gattccgatg attttttgca
     5641 caactttatc ttcataattc agctcaccgc gcattgaatc ttcctgttca gaatcatgct
     5701 ttccttggtt atttgtatga tcagaaccaa gttttccttg tccgtttgtg tggtcagaac
     5761 taagtttttc ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc acaggtttag
     5821 aaggatcgat tgcatcatgt tgttctggaa ctttagattc tggagttcct ggatgatgag
     5881 gtgcatgctt ttctgcatca tgtttcttct cttctactcc ttgttgaacg ttagggacta
     5941 ctggatctgg atgtgcagat actggtgctg ttttgttctt ttcatcgttc acaccatttg
     6001 attggtttgg tgtcgttggg tctggatgtg cagacactgg tttttggttg ttcattttat
     6061 tgttattatt tttgttattg ttattcatgg atgtttcctc ctaagattta aaatagtgat
     6121 ctaagatttt cgtttgtttc agataaaaac cgatagcgac gccgatcgct gccatgatca
     6181 agatcaataa ggtcttgaaa aagcccacgg tgatccataa aacggctaat atcaatccaa
     6241 gtaccccaaa gatgataggg agcttgtatg tcgaaaatag ttctttcatt tttggtcccc
     6301 ccctattcta ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa gcctgtgtat
     6361 ttcaccgata ctttcactgg ctcttttgtt cccaatatgt gttgtacttc attttcgatt
     6421 tctttcatca atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa ctcccctttt
     6481 acattgacgg cgatcttgtt tttcgttgct ttgacattta cttttggtga atggatgaat
     6541 tcatcttcat gcaagtgaga acgaaccatt ccttcaattg cttttttatc caacgtcaac
     6601 tccccatcac catgtttcag tagaaagttt ccgattgttt ttggataaaa caggaaaaca
     6661 agtaacgtga taattgtcaa aatagcaaat gcaacggcaa gccaaaatac atattgttgc
     6721 acgtatgaat tagtgatgga aaagaatcga aacttctcta gctgaaaagg caatggatag
     6781 gttgcactta atagtatcgc aaacaaaagc acagatagca aaagtaatgt tccgatcact
     6841 ccgatcgttt ttacaccttt attcattttc gcacctcctc ataggggtga aacgatcaac
     6901 ttcttataag ttgatcgctt ctccaccagt tactccgtaa atttgggctg taacataact
     6961 tgctttattc gatgctaaga aaacataaac tggtgctaat tctcctggct gacctgcacg
     7021 ttctagtaaa ctttgctgac caaattcagg gatcgaatca gaaggttgtc cttcgtctag
     7081 ttgtaatggt gtccagattg gaccaggtgc tacaccattt acacggattc ctttaggtgc
     7141 aagctgtttt gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg ctgcgtaatc
     7201 taataaatgt tcgcttggat tgaatgcttg aacagaagtt gttgttacga ttgctcctcc
     7261 tgctgggata tgcggaacag cttctttcac taaagcaaac atagcgatga tgtttatcat
     7321 aaatgtgtct ttgacttgct caagtggtaa ctcagcaata gactgacaag aaatttgctg
     7381 tcctgcgttt agtaccatcg tatctagccc gccaagttca ctcacggctt tatctatgat
     7441 ttctttaggt gcttgttcgt cacgaagatc aaaaggtagt aagacaatct tacgtcctgc
     7501 aactttcacg tattttgcta cttcctctgc atctttttct tctcctggga agaattgaag
     7561 agcgatatct gccccttctc tagcaaatgc aatcgctgct gcacgaccga tccctgaatc
     7621 tccaccagtg attagtacac ggcggttttc tagttgatta ttgccttcat aggattcttc
     7681 tccacaatca ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct gatcttgttt
     7741 aggaaatttc tccgtatggt acaagtttcg cgggtcgatt aattttggtt ctgtcattgc
     7801 tgtattcctc ctagttttta aattgtattg ccaaaacaaa tttctttgtt tgatacctgt
     7861 tttaaataga ttctttaatt tgtataatct ttccggtata aatggtaacc taaaactact
     7921 gctgctatca aagagccaaa gactacgagt ataaagccta gagcagaact tttacccggt
     7981 aatcctcctg tattcattcc ccaaatccct gcgaacaacg ccggaacgga aatgattaat
     8041 gcagctgaat ctagatattt catgatatga tttagattat tgtccatcat cccgttgaat
     8101 agatcaccaa tactctctaa taaatctcga tagatctcga tcattttttc tgtttgtcgc
     8161 tgtctcaaat ggacatcata gagtagctgt tcatcgtcta aacgtttgat gaaagtttca
     8221 gaattccata attgatccat tgtttcccgt tgatctctta aggtatgatc gatatatacg
     8281 atgtctcttt caagatctgc ctgtttatac agttcttcat tttccgttgt tttacgagct
     8341 gctttatcca aagcgtcaat tcgctttttc atctctctta gttctttcac gtaatgggtg
     8401 taaatatcta ataatgtata cgtgatgatc ttttcaaacg agtttaaatg atttccatgc
     8461 tttgtcagca gccgatccac aaattcggtt tttctatcga tacaagtaat caagagatta
     8521 tcggaaagta caaaagatat tggcgttaat tttttttcga tacgattatc tgactgatct
     8581 agattaatca ctaccaaaga aaatggatgc tctagtttcg tctcataaag gcgctccatc
     8641 cgagaaactt cttccggata ttcggttcct acgaaaatat catcaggaag ctcgaattct
     8701 tcgattgctt gctcaatctc ttcgggcttt gcactatcaa gtacgagcca attatatttt
     8761 ttttcatctg tttctattac attcttttct tcatccacaa agtagtatct caatgtatac
     8821 ctcctttttg gactttctcg aaactattag ctgaagaagc agttctttaa tttgatatta
     8881 taatcgagta ctgcgatttc attttctaat gaaaagctgt caagctgttt gttgaagtcc
     8941 atgttcaatc gtttaccgaa ataattttgc tctaatttag atgtatagat gtccaataat
     9001 ggatgctcga ttttagtcgc atattctcca aattccgaaa caaaaggaat gatttgaaaa
     9061 aagctgcctt ttgcaaaagc acctttgtcc cttagaaatg gatcggaatc attgataagc
     9121 gcgagtgcga tttttgtatt ttccccaacc ttttcaatta acgtctctac tgcattttta
     9181 gacattgtaa tttccattcc tactccccct cttcagttgt tgtcttctat tatttaacaa
     9241 ctacatctgt ttttggttga acttttgcat attttggata tttagggaat ttagctacac
     9301 aacggaaagc cgtcatccct gcatattttt cgtaatattt cttcgttggc ccttcaaaat
     9361 ccttgctcca cggtttcaca tgtcctgaaa aatggataat gtaaggattg ttgcgagttt
     9421 cttcgtattc acgttcacct gctgctgttg gatgtttttt tgcttttgcc atgatatagc
     9481 cttgtgcatt ccaacgcgga tgaagtggta accaacggtc atgtaagata gcatttaatg
     9541 catcttggtc atggaatcgt aatttttctg gattatgttc gatgaaatct aatacttttt
     9601 gcgtaatatt ttcatctaac cattttttca cattgatcag catcaaacct gagttgaaat
     9661 aacgcattga tttagctgaa attttcattt tttccagtcg ttgatggaac ccagcgtctt
     9721 caacagctgc tacgattgaa tcaccgaagt caagacgcca taatttagag atatcttgta
     9781 aagcgatcat gtcgcaatcc atatataaga tacgttcaac ttctgtgccg cggaacaatt
     9841 ccggaatcgc aatacgataa taagctgttt ctggaatacg atcgctgatt accacatttg
     9901 taaagaattc tttattgatt ttcaagaatt ccacatctga gttcattcgt gcgtttttca
     9961 ctgagaagcg taaaagctgt ttactttcaa aatcgatatc atcgtcaatc acatagaacc
    10021 gaacaaattt agacgggttg caattatcta atactgagac aaataatgca gctaaatgtg
    10081 gtacgaattt tgtgttacaa cttgcgacta ctgctatttc ttttttattc attgatctcg
    10141 cctactttcg ttagagtact gtgtgctaat ttttttaagt ataaattggt atacggatga
    10201 tctttcaacg tattccaagg tttatcatgt cctgtaaagt gaacgatcga aggtttttca
    10261 tttccttctt tatactgacg ttcatatttt tcatttggtg ctggatgacg ttcaaaaatc
    10321 agcgaagtct gcatgttcca ttttggatga agttgttccc aatcttcata aagtacagca
    10381 ttcaaagcat cttggtcgtg atagatgatt cgatcgccat tttcactgag gtaatggata
    10441 gttttttctg taatttcttt tttattccat tgatcaatgt caatcaccat cactcctgaa
    10501 ttgaagtaca agtcatcgga atcgattcct aatctttcca aagctttcac ttggcctgga
    10561 tcgataatag cgccgattgt ttttccattt aacggctcat catatagttt tacgatatcg
    10621 tctagtacta acacatctga atctaagtaa aggactttct tataatcctc tttagctaat
    10681 aagtttggta aagaaattct taagtaagcg gtagttgtga tatggtcact gactaggaag
    10741 tcttcgtaga tatctttttc cactgtcagg aattggatac ttgcattact tgaatattta
    10801 tttaccgttt cttccaatcc ttctttacta tattctgata gaccatcgtc aatcacataa
    10861 aattttatgc gtcttgtttt attgcaattt tctaatgcag ttgcgatcat cacgctcaag
    10921 taaggagcat aattttcgtc tgaagcagtt accacaggaa ctacgccgta tctcttctcc
    10981 atcaaaatca ctcctttttc taatcttttt ctaacctgtt ctacatctag aatattcctc
    11041 atttggtttt aaagcaaaat atatgctttg ttttcattga atattttaaa atttttttat
    11101 cacaaaacgc atcggtaagc gctaactatt cgtttttcat ggctttttat tcaaaggtat
    11161 tgaccaaaaa ctactatata tagccacctt tttcttaaga atgttttttg aaaaaattca
    11221 taatgacaaa gcaagttagt ttcatttttt taaattctca tttaatctta aggtaacagg
    11281 aaaatagata tgtaaaaaga aagtaggaag ataaaatgaa actagcagcg attgatttag
    11341 acggtacctt gcttgatcga aatggaaaga taccagaaga aaatatccaa gcacttcgga
    11401 aatttgatga aaatggcgga atcgtaaccg tcgctaccgg aaggaacagc atttctgcta
    11461 aagatatttt ttccgaatta ggaatcggtg gctatttgat ttcttccaat ggttcactga
    11521 ttgctgaaat gaaggatgga aaaatggcga aagttctcag acgttcaaaa atagatagcg
    11581 atacactgag aaaagccttc ttctatgctc aagaagcgaa aatatccatc attgctagcc
    11641 gagaaaccca agatgatcag ctgacattcg atgaatcaaa tctagtcaaa gatgaccctt
    11701 attatgaaca tttcaattta cagaaccatt cttttgatca aattttaaat atgttggaag
    11761 atccttctct tagctatctt aaattagcat taacagataa gagtgaagaa aaactacaga
    11821 agctacaaag agatctgcaa gcagatggta tccaatcagt attctctgat ccgcatttct
    11881 tagaaatcac gcctacaggt atcactaaag ctcattcact tgtttttctg gcggattacc
    11941 ttggaatcga atcgagtgac atcatggctt ttggcgatca agaaaacgac attgctatgt
    12001 tgaattacag cgggttgagt attgcgatgg ggaatgcaca gcgacaagta aaagaaattg
    12061 caaaccaagt aacactgacg aatgaagaag ctggtgttgc ttatatttta aaaaacttct
    12121 attaaaaaga ataaaatgac gatatgaatg taggaggaaa gaaaatgtta gttattattg
    12181 atatgcaaaa tcatattctt gatccaaaca gcgaatttta tattccagag tcagatcaat
    12241 tggcagaacg tattgctcaa agattagcta aagcacgcca aaataatgaa tatattctct
    12301 tcacacggga tataccagtt gaactgaagg acaaagaaga agaacgagaa gatttgcaat
    12361 tgatccctct tctttctcca caagaaaatg aacgagtgat taaaaagtat tactttacga
    12421 ttccacctga aactttagct gaaatccagg aaactttatt tgaaagcaaa gaagaacaaa
    12481 aagagatcga agtagtcggg attgaaacaa acttgtgtgt actctctaat ctgataggcc
    12541 tgcagagtgc ttttcctgaa gcagactttt ttgttgatcc aatgttagtc tccagtagaa
    12601 aacacggaga atccgcttta gagctgttga aagatttcaa cgtctctatc gtcgaatcat
    12661 aaaaatatct atcttcattt acgatattta acacacaata aaaaaagcac gcagaactgt
    12721 tttagtccta cgtgcttttt ctatcaaagt agattggctt ttttatgaca cagagtgaaa
    12781 cggcaggctc acgacctctt tcttaaatgg tgttcaatca gctgaccctc ggtattagct
    12841 taacaaacgg caaaatatga ggaactattt ttccgtttgt taagctaata ctcaggtcaa
    12901 aacgctgatt tcacaacctc ttccttaaac ggtattcaaa agcttgcatc tgcagtaata
    12961 agaacagcca agtaaaaaca taagaagctg tttttacttg gctgttctta ttacttgatg
    13021 cagggcagct ttttcacaac ctcttatgta ccttgtagga ctcgaaccta cgaccggacg
    13081 gttatgagcc gtctgctcta accaactgag ctaaaggtac atcataaata aaatttattt
    13141 ttgttacttt tcaactatag cacgaatgac aaactctcac aagtttcaaa atataagtaa
    13201 aataatattg ttattcttat ttcttttact tgtgtcataa ctattttgtt actttaattt
    13261 ttatttgctt attttagaaa aaatgttatt cttttattta ttctcaatgt taggagtgat
    13321 tattttgcca tttattgcga ttttattcga tttagttgcg gcagcagctt attttctcca
    13381 attaaattat caaacctcag gaattctgtt tattggaatg atttttcaag gtgtgattac
    13441 ccttttgctt cttgtcatga tgctcagcta taaaggaaaa agatatgccc gtattcaaac
    13501 aaaagtattt gttaaatatg tttccattcg ctatgggatc atcattcttt cctttttagt
    13561 gaatgcgatc gtcctctttt tatatgtatt gaattactta gatatcaatc cattgatttt
    13621 ttctcgatag ataagacaca ttcagcggaa cagttcctgc ttatcagcag aaaactgttc
    13681 tgctttttca tttttcattg atctattctt cttttatttt cctagcattt catttgtgat
    13741 gtatgcaata acatgttatc ttttattata tgaatcgttg aggagtccgt tacatgatta
    13801 cgttggtgag agttttgttt tgggtgcctg cggtcgcact ggtcgcaagt attgtttatc
    13861 ttatgaattg gaacaaagaa cggttttatt tagctatttt gactcttccg gctatctact
    13921 ttatgtggaa agtttttaat tacaactact ttgaacccga tagtgttttt atagaagaat
    13981 tatcaggact ggttttgtct ttattgatcg tcattcttta tttgatacgt ttaaataaaa
    14041 aacattgagt actattgatt gcatgtgtag ctttctatag tcattataac tattctacta
    14101 taatgaaagg tgattaaatg agtatcgaag aagctatttt actggatact ttacgttgtc
    14161 gtctgaattt cttaagaact ggccgatctt tatctcaagt agctgtttct agcgcgttaa
    14221 gtattccttt attcaaatat cgtaagtacg aagaaggaga agaaattcca agcatcattg
    14281 aattaatcta tatcgctgac ttttacgata tttccgttga ttacctaatt ggtcgctcag
    14341 aaacagcaag caaactgaag tgattttttc cagccttggt gctggttttt tcatttaacg
    14401 tctaaggtta acaatctata tataaaggag acatttctat gcgattatgg catgaaaaat
    14461 tgatttcaaa gcttcctcgt caacagcttt taggacaaca tcgggaatgc tgtgcattaa
    14521 gaggaaatgg ctggggaaag aaacatgcta ctgtggatta tgtttttcac tattctcctt
    14581 atagattatt ccaatatcat cagcttgtca tggatgaaat ggaaaaacgt ggttacagcc
    14641 cagcaccaga gtggaaaaat cctatgtatc gtggcaaatc atgcgaagca ttttttgatt
    14701 tacctactgt agagcgcaac gtgccgattt atccggaaca cgatgaagtt tatctagcag
    14761 aatgtttagc aaatttggag caaaaaggaa tttatcttac ctaatataac tagaaaagcc
    14821 ttctatctcc tcttacttca ttaaaaagct ttaaccatct tagatcaagc taaaatgtcg
    14881 taagttttag ggttatcttc atcctcacgt gcaaaactaa aagcgtactc tagttttggt
    14941 tcatcgccaa cttccctaga cataaatgaa catctcctga cgtggtttaa aagtaccgca
    15001 tcttcgacgt ctgtatacga tgcacagcac agattgcttc ttttgatctt tcttttgatt
    15061 tttctgttgc ttcttctaca ccttcttcga ttactacatc gatgcttaca attgcttctg
    15121 cctaatattc ccatgcatcg tcgatttaag cctctgtagt cactgtgatc gtttccacat
    15181 tttaactcct ctgtcgtggc ttcagttgat tttgtgatca gttcagttgc taagccagag
    15241 tcagaacaac ctgccattgc tagcgttaat attgatagta aaaatagttt ttttatcata
    15301 aggttccccc cctgaaaata gactaaaata ataacgcttt ctttttttgt tttcagacga
    15361 aaattaataa tttctaaata ttctttaaaa aagcttataa ctattatgcc ttttttaata
    15421 tataacacag ttatttttaa aaataagaat gctaaatcac tctacaataa cggcgaaagc
    15481 acaaaaaata aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta
    15541 gatctcattc tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt
    15601 ttatatattc gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac
    15661 tgtccgttac ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact
    15721 ttttcattaa ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg
    15781 tcaaactcat tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca
    15841 acatcacttt attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt
    15901 atacactact attcagccca ttcaacgacc aatgaatttt ttcgtacaaa aaaaccgtct
    15961 tttctttcga aaagacggtt aaaagtcaat ttttattaag ctttaacaat gtttgttgct
    16021 tgagggccac gttgaccatc ttcaacatcg taagacactg cttggccttc ttctaaagtt
    16081 ttgaagccgt caccttggat agctgaaaaa tgtgcaaata catcatttcc atcttcacca
    16141 gtgataaagc caaaaccttt gtctgcgttg aaccatttta ctgtaccgtt attcataaat
    16201 aaattcctcc tgatacgtat caatgtacgt tttttcgttg caataaatat ttgataggca
    16261 aaaggagttt tatctatgta gatatatcgc tcaaattacg tttcaaaata gattactcat
    16321 ttaatatatc atgctttttc taataacaca aggataatgc aatgaaatat aaaattcatt
    16381 gtcttctatt gtcttcgaat cgaagaaaaa gttgaataac cgcgattgat tcactttttg
    16441 ttttcttgtt tatttgcttt tatctgcaac ttttgacttt aaaatttctt aactatgaaa
    16501 acctttcaat tggtggtata cttataaatg gaatttcgtt atcaacgcgt tcgttgcctt
    16561 tcagcaagta tttaaagaaa ggagaatggc tatgacattc tataacaaag atatacaata
    16621 tcgagtaacc ttggatacag atttgaatct atttgtcgtt tttgacaaag aaaataccaa
    16681 tcatgtagcg acaggtgtta cgatcgaaca agcagtgcaa gaattaaaaa aatctgcata
    16741 aggagtggaa accctcatat ggacgattct cgttcttctc tttaaacgca tctactggta
    16801 gataacagta gatgcgtttt gtgtttgttc taaattgttc actacaaata aggtagatac
    16861 ttcaaaataa aaaattactt ctttttcatc tgttccctca gtcgtgcagg acaataaata
    16921 aagaggacta gaaaatagtc ctctttatta gagatacaac cctttacttt ctaaaaatcg
    16981 ttttgcttga tgcaggtcat aaaattcata gtctgcttta ttggattcat ataaacttac
    17041 atctactttc aacttatgct tcccattatg atcgggcaac cctatcaaat gatctttgat
    17101 gagaaaagac aaaatgcccg ctcgccttgc tgcttcgatc cctgcaatcg agtcttcaaa
    17161 tacaagtgct tcacttggag gaatattcat ttttgaacaa gctttcaaaa agatctctgg
    17221 ttccggcttt ccttttttta catcatcccc tgaaatgatt tgatcaaaca aggcatatag
    17281 attttccatt tttaaataca tttctacttt tacccgtgta ttagacgaag ccacgattgt
    17341 atggattcct ctttcttttg caaaagataa aatttcttct gctccttttt tcgtatatac
    17401 tctcttatct tctttcacga ttttttcttt atattgattg attgcttctc gccatttgat
    17461 atatggaaca tcttctccgt acaaaatgcc catttgttct cttatcgcca tttctgtttg
    17521 gccagttagt aaataataga cttccttacg catttcataa tcatactttt gtgctgattt
    17581 tacataagca cgataagcta aacgtccagt ttctaataaa aggccatcca tatcaaagat
    17641 gaagagcttg attttattca taatgctctc ccagtggttt ttccttactt atcgcctcat
    17701 atagaccttg catgtaagtg agtcccattg cacgatcata caggccatat ccaggcatag
    17761 ccacttcacc ccacacggtt ctaccatgat cagggcgtat aggaccgtcg aacccttctt
    17821 gcaccaacgc tttcataata tgatacatat caagggagcc ttctgtagag ggatgtgccg
    17881 tttctttgaa ctttctttct cctaaaaact ccacgtttct gaagtggaca aaatggatgc
    17941 gcgaacctac ctcatggatg atttcaacta aatcgttttc cggatttgcc cctaaagatc
    18001 ctgtgcacag cgttagtcca ttatatggag aatctactgc atttagaatt ttcttcaagt
    18061 cgccaagatt tttcgtcact ctaggaaaac caaaaagttc ccatggcgga tcatctggat
    18121 ggatagccat ttttacatct gctttttcgc acactggaat cactttttct aaaaaataag
    18181 tcagattttc aaataattct tcttcggtca ttccttcata cagtttttcc agctgagcga
    18241 attttttcaa ccgttcttct tcccaacccg gcaatttgaa tccctttgac tggctgtgta
    18301 tcaattgata catttctcct ggctccatct gatcgacgat tttctgatcg tataaaagag
    18361 aatagcttcc atcgttgttt tcattaaaca aatcagtttt cgcccatcca aaaattggtt
    18421 tgaagctata acaaatcaat cgaaccccac atcgagacaa gtttttgatt gttcgtatat
    18481 agttatcaat atagtaatct cgttcatctg tccctgcctt gattgcatcg tggatcgcta
    18541 cactttcaat tccaagcagt tccagtcctt ggctctcaac agatgctttc aactcctcaa
    18601 tttctgaaat ctcccaaaca tctccaggca ttttagtcag caaagttcca attatcccat
    18661 ttattcccgg tatttggcga acatatttta atgggatcga atcccctttt tcaccatacc
    18721 atctaaatcc ccacttcatc ttttcagcct tccttctaaa aatcgtcctc atctaatttt
    18781 ttttcttccg ctgaagcatg ccaaacagtt aaattatctt ttccttgttt gacaatttgt
    18841 tccaccgctt cttctatcgg tgtgtgaagc atctgatgat tgattgcttc taacaacatt
    18901 ggcagattca cgccccctat gacataagtg gctgctttct tttcttcagt cagttcattg
    18961 actgccaaga gcgcttgatt gtacggactt gcgctaatta gatcaacaaa tactagcatg
    19021 ggttctgctg tgttgatcat agttaatttt tctttgacct tctctccgaa tgtttggatg
    19081 tcctccccag actctaagca cattgtttca atatggctcg atgtcccgac aattacttcc
    19141 gccgcatctt tcagtccttc gctcaactta ccatgtgtaa cgatcatgat ttccaacatt
    19201 agtcttctca ctccttgttt tgcacttttt acttatcgaa aaagagcaat aatctgattg
    19261 gcattttgcc aattaaatgt tttccctcgt aaatctgttt tggtcaaata gaagaactgc
    19321 gcttgctttt ttcttgcact aaaaatataa ttgacgtttt tttctttcaa cttatcaact
    19381 tgtttcagcc actgttcttt atttgaaaaa taatttcccg aaaactgtag cggtgtatct
    19441 gccccaacag agtctagata aaaaagaata gcctcttttt tcatacccga tctcacgctg
    19501 tctaagccct tttctccata acagccttca tcaacaaacg catacgctac actcggatca
    19561 tcaatcgtac ggatcatttc tagcatcgtt aatatggatg aagtatttcg aataaaagtt
    19621 tgtctgctag gccatccgcg agctgctttt cccaataaga aaaagtagct cccaaatgct
    19681 agtacagtga tacctgttta tacggatagc agatcgaata cgtgatgagg atttttgtta
    19741 tatatcatca ttccccacca acctaaactg aacaacaaaa tgaaggacaa taatatagag
    19801 tagattgttt ttttcctttg gtctttccaa tcaaacatga aataacttcc taatgcatga
    19861 acgggtgtat cgtaataagt acagataact ttctctgctt tttcgatatc tccaacataa
    19921 atattttttg atcggtaggc tagcgtatcg taagcaatga ctgtcacatc tgttctcata
    19981 ctgtaaatat ctgatactaa tgctgaaaga aaacgctgtt tactcttttt tgtacggcgc
    20041 ttacggtaaa tatagcgata acgaaattcc caatcacgaa acagctcgtt cttcttcatt
    20101 ttttcgccct ctttatcttt tttctaagca tttgtttatg acaaatcaaa cttgttcaat
    20161 acatctttta atcctgtttt aggtgcagat ggagtggctt gaaaataaat attctttacg
    20221 ccatattgtt cattcatttc tttcaatttt tcagcatctg atttatttaa ataaacagat
    20281 ttcgtcacta aaagatctgt atcaggattt ttttgagcaa tccctccaat attcaattct
    20341 tccatgggga ttccgtgttt tactaagtcg taaatcgttt ggactgtttt tgcgatcaaa
    20401 atacaattgt attctttaaa ttgatattca tcccaataga atttagcttt ctctggtgta
    20461 atgacaattg ttttttgtcc tgtacggctt cctgcatttt tgtaaagatc tctcataaat
    20521 ttatcttttg ctacaacttc gtctacaaca aaaatggaat tcactccact tttttgagat
    20581 aacgttacca ttacttgccc atggatcaaa cgctcatcca cacgcgctaa attgactaca
    20641 cccatcttat tcacgttccc ttctttttat aaaataccga ttcctgctag cacaattaat
    20701 attccagtca gccataataa tgcttgtgtg actttcaatc cttttttcgt atagaacaag
    20761 tacacaccca ttacgacagc taaaggtaat atccctggca cgatttgatc aagaatttcc
    20821 tggataacaa actcacgtcc ggatatcgca aatttcaatg aagattctac ttttacatag
    20881 tttcccgcaa gcacccccat cataaacaac cctaaaactg atagcatttc aatcgctgtc
    20941 tgaacaccag tgttttgctt taggcctgta gcattacggc ccatagtgaa agcttgtctt
    21001 gcgaaaaata cccctagtaa aacctgatac aacccaaagc agataaaagg aaaaagtgcg
    21061 cctaaaggac ttccctgttg tgcccaaggt accgcgattg cgataaaaat atattggacg
    21121 gttcctgaat cgattgcatc tccgattcct gctagtgccc ccatcaatcc cgctttcgtg
    21181 ttatacatca agtcatcctg catcaagatc tctttttctt catactcttc ttctgctctt
    21241 tgctgttcca tagaagacat taggccagta atcacaccgc caccccagct taattgagtg
    21301 ttgaagaaca gtaactgccg tttatatgct gcttttaaag agtcttcatc tttataaagt
    21361 tttttcaaga ttggcatcat tccgtatagt agtgatggcg ccaagtaacg ttcaaaagag
    21421 tgaggaattt cgtttgcaaa atgccatctc agataggctt ttgttacatc ttttttcgtg
    21481 atttcttcag gagcacctgt ttgattatcc attatttcag ccatttccca ttcctcctaa
    21541 attaaaatcc gtcatcatag tcatcttcgt catcaaaaga tgctgaattg ttattgcttg
    21601 tggcaggctg tacttgtttc cctttttgcg actggacaaa gatcaaagcg ataataatcc
    21661 caaaaatggc ataagtaacc atcgtaatat ctaaagattt caaaacaaca ctcataaaat
    21721 aagcaaggaa gaaaaagacc agataatctt ttcgaccgat aacgtataca gttgtcgcga
    21781 taccgattgc cgctaaccct ccacctacta cttcaagaat gtggataacg actgttccct
    21841 ctaaagcttt gattacatca gcgatcaatg gcgcccctaa atataaagca atgaaaacaa
    21901 gcggaacgaa taataaaaat gaggcaaccg ttggataaag aataattgat cgggccatag
    21961 cttttgtatt caaatcttct acagctttgt ctgtatattt acctaaaaaa gtattgatga
    22021 aaaaacgaaa ctgatacaag taacttccga gcaatccgac tggcaccgca acggcaatcg
    22081 ctgcttctgg cgacagatcc cctaataatg cgactggtac tgcgatagct gctgcaatag
    22141 atggttcagc tggcattgat cctcctggag aaaaaacccc catataaatc atttgcaaag
    22201 cggctgtaac aatcatcgct tgtgcaactt cccccatgat caacccgacg actaatccag
    22261 tcatcaaagg agaaaaacgt aaggtcaacg tggctcctcc acctaaccag cgggacataa
    22321 ccgcggctac ccataaagca atcaacaaac tttgtactac acttaatgtt tccatgtgtc
    22381 ctcctccttc agatctttct gatataacct tccagttcca tcattgactg tatcagtgat
    22441 ttctgtgtta cctctatagg aatcaaatgc actttctctt tcgaatcaat aaattcagcc
    22501 attttatcta ttatctgttt atctttcgga aataatccca tttgttctag agaaaccggc
    22561 agttctaatt cccgatacaa aggatataac tcgtctattt tttcccagcg accttctagt
    22621 gccagttgat agaaaatccc atacgctact ttttctccat gtagaaaacg atggctttcc
    22681 ggaatatatt tactgatagc gtcatgcata gcatgtgctg ccacatttct tgcgtattta
    22741 tctcctaatc ctccaactaa accagcaaca gcaaagataa tctctgaaag ctgtttgaac
    22801 tccgatgtca gttttctttg tgccatatct tggattgctt tagcagaatg attcaaaatc
    22861 tcttctttgc agatcttcgc agtatcttgt gctagcttca aaaatgattc atcttgcaaa
    22921 aaatcttgtt ctaagatgag ctcagattca taccattttg ctagtgtatc tgctaacccg
    22981 gcaatgaaat atggaacagg ggaatcaatc actaattggg gatctgttat aaaaaaagca
    23041 gcttgtctgt aaaagtgttc tgttttccct tctgcttgtc cattctcctt atacatgaca
    23101 gcaagaggtg tccatggagc acaattacta gctaaagtag ggatgactcc gaaatcgatt
    23161 cttgcaagat tggcactata acccactaga tcggtcagtt ttcctccgcc aacgccaact
    23221 acgaaatcga tttcattttt ttgatgattg tagctattcg tctggcttca gcataactac
    23281 attctccaga ataacgatga taaaaaaatt gaatcgtttc gtcattcaaa aatactaatt
    23341 taggtagcgc cttttcccat gataccgttc cgtgggtgac cagtactctt ttcgcgccgt
    23401 atgatttcaa gagatcagaa acttttgaaa taccaccttc ttcatataaa taaaattgag
    23461 ggcctgtttt cacttttaaa ccgttcacca ttttcttcat catcctttta tatagttgct
    23521 ctttgtctgg acagaacgtt caggaagaag gtgctgactg atctgttcga catctctgac
    23581 acatttttca cccattgctt ctaaacattc ccttgtgtag gccgatatat gtggcgtaat
    23641 cactacttgt tcaaacgcta aatacggatg atcttgacgt cccggttctt cttctaaaac
    23701 atctgtggca tatcctgcga tctttccgct tttcagacca ttaatcatcg cttcttcatc
    23761 aactagagcg cctcttgcac tattagatag ataaacattt tctttcatct gatcgacttc
    23821 tttatcagaa atcatgtgat agttctcttc tgtcaaattc gcacataaac aaatgatatc
    23881 tgattgttcc agtaattctt gaagttctac tttttttgca ccaaagcttt cgatgtataa
    23941 atctgatttg taaggatcat aagctagaac atcacagcga aaaccgttac gcaaaatttc
    24001 gattactccg cttccggtat ttcctgcacc aatcacacca actgttttgt taaacagcgt
    24061 cctaccgacg aactccgccc gatcttccca tcgatcgtcc ttgactcttg cttgtgcttg
    24121 tgtcgtgcgt cgaagcatgg acaacaaatt agttatatta ttttctgcta cagcatttcg
    24181 ttcaactaaa gcaggtatga tagatacgat tgtctggtgt tcttttgctg cttttaaatc
    24241 aatattgttg taaccaatac catgtcggga aatcaatagc agttcatctt tgttttcaaa
    24301 aaaatctcgt cgaaaaaatg gcgtaacact tgcaataatg atattgaatc ctttaagtaa
    24361 ctccgctagt tcttttcctg gaatctcttg atcaacagta aagaaatgaa cgtctccaac
    24421 tgactttaat ctttccaagt ggtgtggaaa gactcttccg aaactgctgg aattaacgat
    24481 tgctatatga taatctggca ttttactcac ccttttcaac tatttcatgt ccgccaaacc
    24541 catttcttag tgctgaaact acttttgctg aaaaactatc ttcttcctgt gaaagattcc
    24601 tcatgaacaa actagttgca attactggaa ctggaacagc catttctagc gctgtttcaa
    24661 ccgtccattt tgcttcacct gatgcagaaa cgattccccg ataattactt aaattgggat
    24721 gttcgtttaa ctgatcttcc atgatttcca tcagccagcc gcgaatcacc gaaccatgat
    24781 tccaaacacg tgcgacttgt tccaagtcaa aagcatactc acttttttgt acaagttgga
    24841 atccttcacc gattgcttgc atcattccat attcgattcc attatggatc attttcaaga
    24901 agtgtccgct tccggatgca ccagcataca gatatcctct ttcaattgct acatctttga
    24961 ataattgttc cacggataga aaagtttctt tatctcctcc aatcattaag caggcttgat
    25021 gtcgtgctcc ttcgattcct ccagaagtcc cacaatccaa gtaaccgatt tcatttttct
    25081 gaaactcttc tgctcgacgg attgaatctt tataataaga gtttccgcct tcaataataa
    25141 tgtctcctgg tccgagtttt ggaaaaagtt ttcttaacat gttttcggtt atctctccag
    25201 caggaagcat taaccagatg atttttttct gctcaaaagt agctagaaat tcagagatct
    25261 cttgataagc aggaatgtca ttttctcttg cctgactaac agctgtactt gaaatgtcga
    25321 gtccttgtac tttgtgatgg tgctctttta agtttaaagc taaattcaat cccatttttc
    25381 ccaaaccaat tacaccaatt tccatgatag cgcctccatt tcttataccc tcattataca
    25441 aaaaaatttt ttgtaaatag attttacaaa aatatttttt gtttgatttt ttcctactct
    25501 ccttgtatct ttaacattaa tgagaacgga aaaaatgata atcttattat gaaaatgagg
    25561 aattctcatg agcgtctcca taagactaca aatcaaaaca atgtacaacg acttcagccc
    25621 aaaagaacag gctatcgccg attatatttt agaaaatcca agcaaagtaa gtcatagtcc
    25681 tattagcaat ttagcaagtg aactgggcat tgctgactct actttttttc aatttacaaa
    25741 aaaacttggt tataatggat ttaaagattt taaaatggcc atgttgatcc aagaaaatga
    25801 tctatctgct atttctatcc acgaaaatat tcagaaaaca gatactgagc tcactatggc
    25861 gcaaaaggtg ttcgagtcta gtatttcaac tttaaatgac acaaaaaagt tgttacaaca
    25921 aaaggatcta aaaaaagcgg ctactattat caatgaatct aaacgtgtct attttttcgg
    25981 tgttggcggt tcagagattg tagcaactga cgcttatcat aaatttttgc gttcccctat
    26041 cgcaacttct cacagcacgg attaccatat tcaattgatg gaagcttctt tattgacgga
    26101 aaaagactgt gctgttttga tttctcacac agggcaatca aaagaaacaa tccatattgc
    26161 tgaaaccgta agaaaaactg gagcaaaaac aatcgttatt actagccaag ctaattcttc
    26221 ccttgctaaa cttggcgatg tcgtttttat ttctatttct gaagaaacag aattccgatc
    26281 tgaagcttta gcttcccgta tttctcaact aagcattttg gactctctat atgtgatttt
    26341 gatgttttac aacgaaaaag aatcacgtcg ttctatcgct aaagttcgac atactatttc
    26401 tgaaatcaaa gaatagcacc ataacagaaa aagcgaaaaa tccataaaac aaaaaaagcc
    26461 actaccaaat atttggtagt ggcttttttg gttctgcaag ggtggtacag aacatcaata
    26521 gtcggtgggt tagaacgtat gtcatcgcat ctttggagaa ggttgtattg gtattattcg
    26581 agttacttgt tagatgcgat aacaaagtta gtatacagta gtaaaaaatt tatcttttct
    26641 tttttttatt atttttgttt cttattttct aaaaaattaa aaaaaatttt ggtgaattaa
    26701 gtatttcctt gataacactg gcttttggcc tcattttagg ttatcatttg taattttatt
    26761 tcacagttga tatgattgtt atgtaaaata aaaaagttta ggatacatcg aatttttggg
    26821 gaaattcttt tgggggtatc ctaaatcttt tctattatat tcccatattt tctattattg
    26881 gtctagacgt atgatggtaa actttgtacc tatctgattg ctgaatcatt aattgcttat
    26941 cattagccat ctttgtttca agttcgctag atgatcattt tattcacatc ctaacaaact
    27001 tgtcacattt atagattctt aatctattta tcatacaatt ataaaaaagg aggtgctgct
    27061 tatgaaagct atccatattt ctttgctttt tttcgctagt gcattgattt tatttgctat
    27121 tcatctttgt tcccagcctt atatcaataa caacggaata ttagtggaac cagcattctt
    27181 ttgcttacca ctcggctttt tgtcccttct tgcctccgca ggcttcggat ttgttgcttt
    27241 ttttaaacga tcaaaacaac aaatctgact tataaaattt acgtaaaaga gatcgtgaca
    27301 ataatcgtgt gatattccca aatttcgaat aaaaggtgcc ccttgccatc tctaactcct
    27361 cagtcacaag ttacaatatt cataaaccat gaaaagcttg tttattgaaa ttgctccttg
    27421 tgacttggag ctaagtgaca gattacaacc tcttttgcat cataagactt tactccttga
    27481 aaaacattac taacttttga tatgtttctt gcgcttgttt attgctgaaa tcaaactgat
    27541 attcatggtt caaaacagca tttttctccg cataatctac ttttgtgact ttcacccccg
    27601 ctttctcaaa tacagaagtt acttcttcca tttgtttcgt aaacgtattt gtatttccat
    27661 ccgttaagaa tgtgttttgc gtccaacgat ccgcatgtct gaccaaggct aaacttttga
    27721 ccttctctga ttgttcaaag tccttattat caaagtaagc ccatccacat ttttcataaa
    27781 gaaagctaga gattttatca cttacttgag taagtgcaga aggatccaac agaccagaca
    27841 aagaaataaa cttcgtaatc tgcttgttct ccaaagtcgg atcaagatga tcagcttttt
    27901 taatttcttc attttgaagt gctaacaaat attctccact aatttgtgca cctgccgaat
    27961 ttccaccaat cgaaatattc gaccaatctg cttgatcttt tatctctttt ttagctgctt
    28021 gaatggcttc gttcaattgg ataactggtg taggatgttt tgcttccggt gatcgttcat
    28081 aatctatatt tacgatgatt tgctgcgtgt ctgaacatag atattccaaa taatcacgac
    28141 aatcattttt atcccctcct acatatgcgc ctccatgcat ccagaaaaga atcggtaatt
    28201 ttttcttttc cactttcggt gtgtagatat ccatattatt atttttgtac tttgaaggat
    28261 aggatatttc tttttttata tcgatttgtt cgattttttg ctggaaatca tctggtcggg
    28321 catatacttg ttctttttca cttgtgtcaa attgttttct aacaagcaag ctaatcggct
    28381 ttggtgtgat ttgattaatc aaaaatacag tgactaaaat aactacgatg ataactggta
    28441 ctgcaatcac tattcgcttg aaccatttca ttcatttacc ctctctttct tcgccaaatc
    28501 catgtgataa tcgtgctaac agataaaaat ccacctgaat aaatgagaag cgaatgtttc
    28561 ttttctccag ttttcggcaa cattcttttt tttgcatctg tctttttttg tactttcact
    28621 gtcatctgaa tcggttgcgt atcatcactt gattgatcct ctcgaggttc attctttccc
    28681 ttattgtctg cattctctgt cggatcttgc ttttttggtg gagctactgg gatttttttg
    28741 taatagaaaa taatagttgg tatcgtctca ttaaaaatcc cttcagtcgg tccttcaaca
    28801 tttgctaatt cgtatccttc gaatgtcttt tgctctgtca tataattttc tccataagct
    28861 ccgtaaagct ggatttcgtc ctggtctttg agtagacttt tattgttttc atccacatac
    28921 tttacgttta tctgatcatg gaacactact ttcaaaaagt ccgttacatg tttcgtgtag
    28981 gcagcatggt caacttcctg tgcctttcca tgccctgctc cttctactaa taaaagatct
    29041 ttataccctc ctgataactc atataatttt tgcgcgttag acacaggtac tacatcatct
    29101 gctgttccat ggataatcaa tttaggcaat gaatctgaca ttccaatcgt aggtagagca
    29161 gcttttgtat ccaaatgcat ctcctgatca aaatagtatt gtttcaaaag gcccataaat
    29221 aaatcttcat gctcttgatc cccaatgatg tctaactgat tttttatggc atccggtagt
    29281 gcgggggcta ctgtctggct gtacgtaaaa cgtagttgtt catcaattga tgcaaatccg
    29341 caattttcaa taattccttt gacattgaca ggaatatctt tcgctaatac agacatcatc
    29401 gtagctgctc ccatcgaacc accataaagt aaaatctttt gagatggccg ctctcgaact
    29461 tcttgattta tccagtaaag gacgtcatct gactcatatt gtccataggt cacataatcg
    29521 cctccgctat taccagtcgc tctggaatca acaaataata aattataccc ggccttataa
    29581 aaatcattgt attcttctgt aacgatcccg ttattccaat tccctcgaaa tccgccatgg
    29641 atcatgactg ttttatctga accttgatcg acataatatg catatacatc catcattttg
    29701 ccatcttgtt tgtttagtac agtttgatga agttgttttg tatttggata gaagcctgtg
    29761 ttttctgtag gcgttgtccc atcatctgta cctccaagca tcgacatcaa cacactgttt
    29821 tttgcaaaag cgatatcgta caatagataa gtagcaatca gatgtttgat aatctcttta
    29881 tctacaccat aatcattaac agcaagctca gtgactaata actctattaa ttttgacttg
    29941 atgtcttctg ttggtccatt aagtatttct gtcatctgtt taccgaagta attcaaatat
    30001 tctttaccat tgaaattttc acctttaccg atatcatcat tttctgtccc attctctttg
    30061 tcacttactt tagcagaaga ggattcgtcg tttgacattt ttgtagctgt tgcttcttct
    30121 gtttgttggt tatctgtggt agcggtcgtt tcttgatctg tgctagtaac agatgtctca
    30181 accgttgttt cagtatttgt ttctgtttgt gaagtactcg atttcgtttc atttccagca
    30241 tcagtagttt ctgtggtttc tacctcccga gattccgatt cggaagaaat gctctctcca
    30301 tatactgtcc attgcgggac gcttgtactt acagcaaata agatgaccaa cgctcccttt
    30361 aatacttgtt ttgttctcaa gctattcact cctttttatg tttctaggag aattatatgt
    30421 aaacgcaaac attttttata caaaaaataa atggtttatc ccataaaaat agaacaggat
    30481 tattttctca ttttacgtaa taaagcagaa tggataaacc agtatccatt ctgctttttt
    30541 taaacgaaga aacaacttat tgctccaaaa atgtcagaaa tcgtttatcc caaattttct
    30601 cgtaaaaagc gattgtttcc tttgctttca taaaaggatt atcataagtt gtcgttgctc
    30661 ctctacacat cataagctgg tatcctaatc catgagcaaa cttcacagtc gtatctatac
    30721 aatactctgc ttgtgcccca cataattcga tcgaatgaca gtccaattca gttaataaaa
    30781 cactcaaatt agtcttataa aatgaattcg catgggtttt tctcacaaaa taactgtgct
    30841 caggaataga taattccgac aatagctccc atggttttgt ttgtggcact aattgagcat
    30901 cttcgtgttg aacgaaaata actggtttcc caatcgtttc gtatgttttg attcttttat
    30961 tcactaatgc tgtcactgtt tctaattcgt gtatcggcga agaatcaaaa catacccctt
    31021 tttgtaaatc gatcactact attgcgtcgg ctgtgtttga catacttgtg ccttctttct
    31081 tttttcttga tttaatagta tttcttatct agctagttcc attatacgtg acaacatttt
    31141 cttattttac acctctaaat ggcacttttc gactatttct tcatgttatt atttcataaa
    31201 tacttagcta ttggaggaat gtatgatgat taaattgtat acaggttctg gctgctcgtc
    31261 ttcaagacgc gcaaaacgtt ggcttatcgt ccatgatata ccgtttatcg aacagaactt
    31321 aattcgtcaa ccactaacca aaaatgaatt tatccactta ctaaaattga caaacaacgg
    31381 cgtgaccgaa atgctctcta ctcattctat tagttataaa aatcttgttc acagaataga
    31441 tgaactcagt ttgagtgaac tatatattga gattcgtaca tccccttctt tattgaaaaa
    31501 accgattatc tacgaccaat ctgcaaaact tttgactgga tttagcgaag ataaaatcag
    31561 gatgttcctt tctaaaacta aaagaaagca agagctcaac gaactcttgc tttctggtca
    31621 tcttgtcgat tttaaagaaa ataatctttt tgcaatgtct taattttggc ataaacaatc
    31681 tgcttttc
//
LOCUS       DACDTH010000030        29982 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDTH010000030 DACDTH010000000
VERSION     DACDTH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.030"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(213..1940)
                     /locus_tag="IWK54_001457"
     CDS             complement(213..1940)
                     /locus_tag="IWK54_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9593582.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1933..3126)
                     /locus_tag="IWK54_001458"
     CDS             complement(1933..3126)
                     /locus_tag="IWK54_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9593583.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3313..3957)
                     /locus_tag="IWK54_001459"
     CDS             complement(3313..3957)
                     /locus_tag="IWK54_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9593584.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4051..4185)
                     /locus_tag="IWK54_001460"
     CDS             complement(4051..4185)
                     /locus_tag="IWK54_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9593585.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4187..6319)
                     /gene="feoB"
                     /locus_tag="IWK54_001461"
     CDS             complement(4187..6319)
                     /gene="feoB"
                     /locus_tag="IWK54_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9593586.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6316..6789)
                     /locus_tag="IWK54_001462"
     CDS             complement(6316..6789)
                     /locus_tag="IWK54_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9593587.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7190..7414
                     /gene="nrdH"
                     /locus_tag="IWK54_001463"
     CDS             7190..7414
                     /gene="nrdH"
                     /locus_tag="IWK54_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9593588.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7573..9732
                     /gene="nrdE"
                     /locus_tag="IWK54_001464"
     CDS             7573..9732
                     /gene="nrdE"
                     /locus_tag="IWK54_001464"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9593589.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9782..10747
                     /gene="nrdF"
                     /locus_tag="IWK54_001465"
     CDS             9782..10747
                     /gene="nrdF"
                     /locus_tag="IWK54_001465"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9593590.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10836..11975)
                     /locus_tag="IWK54_001466"
     CDS             complement(10836..11975)
                     /locus_tag="IWK54_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9593591.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12284..12997)
                     /locus_tag="IWK54_001467"
     CDS             complement(12284..12997)
                     /locus_tag="IWK54_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9593592.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13251..13952)
                     /gene="nagB"
                     /locus_tag="IWK54_001468"
     CDS             complement(13251..13952)
                     /gene="nagB"
                     /locus_tag="IWK54_001468"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9593593.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14186..15718
                     /locus_tag="IWK54_001469"
     CDS             14186..15718
                     /locus_tag="IWK54_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9593594.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15909..17111)
                     /locus_tag="IWK54_001470"
     CDS             complement(15909..17111)
                     /locus_tag="IWK54_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9593595.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17193..17882)
                     /locus_tag="IWK54_001471"
     CDS             complement(17193..17882)
                     /locus_tag="IWK54_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9593596.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(17988..18356)
                     /gene="rplL"
                     /locus_tag="IWK54_001472"
     CDS             complement(17988..18356)
                     /gene="rplL"
                     /locus_tag="IWK54_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9593597.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18409..18912)
                     /locus_tag="IWK54_001473"
     CDS             complement(18409..18912)
                     /locus_tag="IWK54_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9593598.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(19322..20011)
                     /gene="rplA"
                     /locus_tag="IWK54_001474"
     CDS             complement(19322..20011)
                     /gene="rplA"
                     /locus_tag="IWK54_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9593599.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20115..20537)
                     /gene="rplK"
                     /locus_tag="IWK54_001475"
     CDS             complement(20115..20537)
                     /gene="rplK"
                     /locus_tag="IWK54_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9593600.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20880..21548
                     /gene="sdaAB"
                     /locus_tag="IWK54_001476"
     CDS             20880..21548
                     /gene="sdaAB"
                     /locus_tag="IWK54_001476"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9593601.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21561..22433
                     /gene="sdaAA"
                     /locus_tag="IWK54_001477"
     CDS             21561..22433
                     /gene="sdaAA"
                     /locus_tag="IWK54_001477"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9593602.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22524..23543)
                     /locus_tag="IWK54_001478"
     CDS             complement(22524..23543)
                     /locus_tag="IWK54_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9593603.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(23716..24603)
                     /locus_tag="IWK54_001479"
     CDS             complement(23716..24603)
                     /locus_tag="IWK54_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9593604.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(24680..25225)
                     /gene="nusG"
                     /locus_tag="IWK54_001480"
     CDS             complement(24680..25225)
                     /gene="nusG"
                     /locus_tag="IWK54_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9593605.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25326..25496)
                     /gene="secE"
                     /locus_tag="IWK54_001481"
     CDS             complement(25326..25496)
                     /gene="secE"
                     /locus_tag="IWK54_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9593606.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25520..25672)
                     /gene="rpmG"
                     /locus_tag="IWK54_001482"
     CDS             complement(25520..25672)
                     /gene="rpmG"
                     /locus_tag="IWK54_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9593607.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25834..26478
                     /locus_tag="IWK54_001483"
     CDS             25834..26478
                     /locus_tag="IWK54_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9593608.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26534..27037)
                     /locus_tag="IWK54_001484"
     CDS             complement(26534..27037)
                     /locus_tag="IWK54_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9593609.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27050..27096)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27634..28599
                     /locus_tag="IWK54_001485"
     CDS             27634..28599
                     /locus_tag="IWK54_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9593610.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28698..29870)
                     /locus_tag="IWK54_001486"
     CDS             complement(28698..29870)
                     /locus_tag="IWK54_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9593611.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29857..>29982)
                     /locus_tag="IWK54_001487"
     CDS             complement(29857..>29982)
                     /locus_tag="IWK54_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9593612.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9128 a         6174 c         5353 g         9327 t
ORIGIN      
        1 actttaatca cattagtctt tcatacctac gatcgatctc tttgactcgc acccataaat
       61 tcataatagt ttgtcggcgt ctttggacgt ttcgaagatg agctacgcat tctctttttt
      121 tatagacaaa aaagatagag acgaacatgg agcttccaaa tttggatatt tgatccaatt
      181 ttggaagctc catttatttc gtgtctgtct ttttattttt tccagctttc gactaattct
      241 tggttctctt tgatccagtc ttttgctgct tgctgtggat cttttccatt gttgatttcc
      301 aacataactt tttccatatc tttttctgac caatggaaat tgtctaatac tttgtaagct
      361 tctggctggt cttctttcaa tccttttcga accatcgtat ggatctgctc ttcgcttccc
      421 attccgtttt caggatcttc taagtatttc agatcatatt tggcaaacat ccaatgaggt
      481 gtccatcctg taactacaat tggttcgtgt tttttgatcg cttgacctag tgcaacggtc
      541 attgctcctg aagaagatgt ctctactttc cagtctttca agttgtcata tttttggatc
      601 gtgttttcag cagccgtaac tacaccagct cctggctcga ttcctatgat tttcttacct
      661 gcctgatcgg ttaactcatc gatcgaattt acatccatgt aggctggcac tgctaaacca
      721 acttttgctc ctgtcaaatt ggcacctagg tcttcgactt gatctttata ttgtgcatat
      781 tgtgaaccat gagttttagg tagccaagct gaaaccatgg catctgactc gccatttgaa
      841 acagatttcc acatgattga attatctaat ggcgtcatcg ttacatcata gcccatttgt
      901 ttcaatactt caccgactac atttgttgaa gcaacttctg tatcccattc aacataagat
      961 aagttgatac gttttgtttc tttggcagat gaaaaactcc ctataccaat cgcacctaag
     1021 atcactacta ctacagcacc aatgatcagt ccttgacgtt ttttactttt ctgtgttgtt
     1081 gctcctgcag cttttttatt gtttggctgg ttcaaatgct gtgtaaaacg gtcaatgatg
     1141 attgctaaga taactaatgc aaccccgttg acaaatccat tcccgacttg tgctctttgc
     1201 aatgctgata atacgccgcg tcctaagccg ggtgcaccga tcatagaggc aataacgacc
     1261 attgaaagtg acagcattgt tgtttgattg atccctgcca tgatcgtact tttagctaaa
     1321 ggcaattcta gtttgaatag tttttgacga cttgtgctcc cgaatgaatc agaagcttct
     1381 actaattctt taggaacttg tcgaatccct aaattcgtaa aacgaacggt tggtggcaga
     1441 gcaaagatga ctgaggcaaa tacccctgga accatcccga taccgaaaaa tgcaactgct
     1501 ggaatcaaat aaacgaatcc tggcattgtc tgcataaagt caaggatcgg tttgataata
     1561 ctttgggctt tactgctttt tgccatcaag atccctaatg gtaccccaat gatgatcgag
     1621 atcacactag ataaaagaac aagtgtgaca gtattcatca aatcattcca tagaccttgg
     1681 ttataaataa accatagacc gattaatgtg aacaacgtaa gtcctggacg tttcttagaa
     1741 ataaagaaag ctgctaccgt caataacaag ataaacaaag gagctggaat cactaacagt
     1801 aaatttgtaa tccccgacat cactgcctgt ccaatcgttt gtaaaaatga aaataggcca
     1861 gaaaaagttg tcgtaaacca ttctgtgata ttttctaccc agtttgccac tggtaattga
     1921 tagttcaata gattattcat gttcactcac ctcgcttgtt tctgctaatg cttcaatcac
     1981 actacctcgg atgacgacac cttttaattt tccatcctct acaactgcaa gtggagtcgg
     2041 tgagtcataa ataatattga aaatatcact taccaccatg tctttcccaa tcaccgtcac
     2101 attttcatct aggaagtcga tcaatggacg tttttgcttc ctagcttcta acgcttgatc
     2161 tgcagtgatg atccctttca ggtgtctctt tctgtcaaca gccatcagca tgctgacttc
     2221 ttcattacgc atccttgtca atgcaacgtt tggtccatca gattcgatat tcgtcgtcaa
     2281 tgcaggtacc atgatgtttt gagccgtaag cactttggaa cgatcgactt cttccacaaa
     2341 ttcacgtaca taatcatttg ctggattggt caatatttct tcacctgttc cgatctgcat
     2401 gatttcgccg tctttcatca aggcgattct gtccccaatc cgtaaagctt cattcaaatc
     2461 atgggtaatg aaaataatcg ttttttgaac attggcttgc aggtccagta attcgtcttg
     2521 catttctcgc cggatcagcg gatcaagagc cgagaacgct tcgtccatca gcaagatctc
     2581 aggatcattt gctaaagctc tggctaggcc aacccgttgt tgcatcccac cagataactg
     2641 actaggatat tggtctttaa atgaaagcaa actagaattt tctaatgctt tttctgcttt
     2701 ttcttgtcgt tcttctttag gtacaccacg gacttctaac ccgtattctg tattttccaa
     2761 gatcgttcga tgtggaaaca gaccgaagtt ttggaaaacc atattgattt tatgacgacg
     2821 gacttctcgc aattcttctt tgcttaactt cgatacatct tctccatcaa tatagatact
     2881 gcccgaagtt ggctcaatca gacgattcaa tagacggatc aatgtggatt tcccactccc
     2941 ggaaagcccc atgatcacga agatttctcc ttcttttacg tcaaaattca cgtcatacac
     3001 accaaccgtt gcaccggttt tctttaaaat ctccgttttc ggctggtgat cattcatcat
     3061 atctaaagcg gcttgtgtct ttttaccaaa tatctttgtt aaatgtttta cttctacttt
     3121 taccaaacaa attctccttt tttatgtaaa ttcatttttc tttctgaaaa aagtgaccac
     3181 actatgttaa catcatgtag tcactttgtc aaaaatgaaa gaaatttgct tgtccaaact
     3241 gaaaaaaact tggctaaatc tttgtaaata aaaggtttag ccaagttaat atttttttaa
     3301 tatttcacac aatcattctg caagctgatg ctccgggtaa aaaaagtttg tcatacgctg
     3361 tagtgtatat tcatttccaa taaaatgaac gatgtcccct gccgtaaatt tagcataggg
     3421 gccaggtgaa acaagcagtt cttcattatg agaaattgcg ataactgtgg cgccagtcgc
     3481 ctgccagata ttcaactcgc taattgaacg gtccagatgc attgcttttt ctgataaaga
     3541 taattcaaac ggaagtaaag gattcatctt atgtattcgc ttcgtttgat tgaccagctg
     3601 atccaataat tgagaaaaat gattcagctc atcttgttgc ttctccacac ttgccatgat
     3661 ttctgctttt gtatcttgga tggattgtac atcttgatat tgcaatagga aatcttttgc
     3721 tttttccttc gaagcaacaa atgccccact tccatgccgt acttccatga ttccaagatc
     3781 taccagcaca ttgatagcct tgcgggtagt ctcaggagaa gcattgtacg tactggcaag
     3841 tgttgagcgg gcatggatct tctcacccac tttatatcgg ttttcaacga tacgttcggc
     3901 taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt tttttctttc cagacatttt
     3961 ctttcctctt tcttttttta ctttccctca tcttactatg ctctagcaaa ataagcaaaa
     4021 aaggggagaa tagcctcttg caacgactga tcatcgcttg atctgttcct tttttactgg
     4081 gcaagctgtt tgacagtcat cacaattctt accggatttt aaacgagtat agacaattgc
     4141 gcctgcactt ccaaagatta acacacttaa taagaatgtc gccatcctat tcctctcctt
     4201 ctaatgacaa tatagtataa aactggttct tggattttgg ctgacggaac aaaacataaa
     4261 cgagtcctat catcagtaaa atagcaagat acgtccctaa cgctacctgt ccaccctcca
     4321 ataagtgtcc caactgataa atcacaaaac taactaaata agctaaaccg cattgatagc
     4381 ctatggcgat ccaagtccat ttcttggcat tcatttcccg atggatagcc ccaattgccg
     4441 caaaacaagg cgcacaaagt aaattgaagg ctaacaatga ataagctgct gcgggagtat
     4501 atgtgtgttg tagcacttgc cagatttctc tgccattttc ggatacttcc gcatgaccaa
     4561 acaaaatacc aaatgtgcca ataatatttt ctttagcgac caatcctgta atcgctgcga
     4621 ctgttccttg ccagtttccc caaccaagcg gctgaaaaac aactgccagg attcgcccga
     4681 aaaaggctaa gatactttgg tcttcagaaa caggttgagc ataaaaatta taagaagagc
     4741 tgaaccagat cacgatacta gaaacaaaga taatcgttcc tgctttttta acaaaagact
     4801 tgctgcggtc ataggtttgt cttaggaccg ctgaccattg aggaaaatga tatgcgggca
     4861 attccatgat aaaaggagct gtatctccag caaacaaacg tgtttttttc aagctgattc
     4921 cagagaaaat aattgcagtc ataccaagaa aataggcaga aggagctacc caagattgat
     4981 gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa ctttgcagaa caaggcatga
     5041 aggttgtgac catgatcgtc aaacgacgat cttgctcgtt ttcaatcgtg cgactggcca
     5101 tgacgcctgg cactccacat cctgtagcaa tcagcatagg aatgaaggat tttcctgaaa
     5161 gaccgaattt cctgaaaagt cgatccatga caaaagcgat cctcgccata tatccgcagt
     5221 cttccagaaa gctcaagcat aaaaacaata taatcaattg cggtagaaat cctaacactg
     5281 ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg catccatccc gctacttgcc
     5341 attcagctaa caatctgcca acatgttctg gtaaccatgt accaaacaat gtgtcattga
     5401 tccaatctgt ccccattgtt cctactgttt gaatagccag ataatagatc agccacatca
     5461 caaatgcgaa gatcggcaag gctaaccaac gattcgttgc aatctgatcg attttgtcac
     5521 tcatcgataa ttggaaagaa tcatttgaca caacacagag tgtacatagt tgggtaataa
     5581 attcataacg ttcattgacc agaatcgtct cactatcgtc gtccaatagt ttctctgtta
     5641 actggatcag ttgctcgatc tctttttgct gagaaactgc tatctcgtaa tcctctctta
     5701 ccttctgatc tcgttcaaaa tatttcattg cataccagcg tttcttgccg ctttgttctc
     5761 ctagtacatc ttcaatttca tgtaaagctg cttctaaacg tggatcatat gtacagggaa
     5821 caggaataga aggccgcttt tttgcagact cctgaatctt ttcgattagt ttttcgattc
     5881 cttgcttttt caatacgctg attggtaaaa ctggtacacc aagaccatag gaaagctttt
     5941 ctagattgat tttttttcct tcttttttga tgacatccat catattcaga ccaatgatca
     6001 taggaatgtc taaatccaat aactgcgtag ttaaataaag atttcgttct aaattggtag
     6061 catcaataat gttgatgatc agttcaggct gttcctgaac caaataatct cttgcaatca
     6121 cttcttctgg tgtataagga gacaaagaat agatgccggg taggtcctga atatgaatgt
     6181 tcggattttt tttcaacgat ccttcttttt tttctactgt tacgcccggc caattcccta
     6241 catattgatt cgaaccagtc aagagattga acaagctagt tttcccgctg tttggatttc
     6301 cgactaacgc gatattcatt ctttttcctc cctttgtatc aagattagtt cggcctctga
     6361 ctttcgtaac gtcagttcat accctcgcac tgtcagttcg attggatctc ccagaggggc
     6421 tagttttcgg ataactattt ttacatttcg cgtcaatccc atatccatta accgtcgctt
     6481 cactgcgcct tgtccgtgga ttgctacgac gatcccagtc tctcctgctt ttaattgatc
     6541 aagagaagtc cagctttcag aggaagcagt ctttttattg acaaagattt gacctagtat
     6601 ttcccgactt actgccactc gatgactttt taccagtaaa atggcattgt tgcctgataa
     6661 attgacgacg acgattttac ttccggcaac gatcccgata ttttgcaaat ggcgttttgt
     6721 tttttcttct gtttcgattc gttcgacttg ataaacgaaa ttctgctttg cctgcgctaa
     6781 ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt attaaaatca ttcattatat
     6841 gttctaattt tcttacgtgt tacgcaaata aatgagaatc attctcacat agaatactct
     6901 cggtaattga gaatgtcaat tatacttgtg atattatgag cgaatcttta gtattgatag
     6961 ataaaaatga gaaaaaaata tttcttcata gttttccaca attttttctc tattaaattt
     7021 gctattttcc cacacgatac ttgttttcag ctcttttttc aaaaacacaa aacttagtat
     7081 tccacagttt ccacaacaca atatgtagtg tttgttgttt gactttttta ctaagagttt
     7141 tatactatct attatagata ataagaatgt gttagaggag gcactcacca tgaacgtaaa
     7201 aatcttttct aaaaataatt gtatgcaatg taagatggtc aaacggtttt taacagaaaa
     7261 tcaaattgct tttgaagaag tcaatatcga tgaacagcct gaagcagtag actggttgaa
     7321 agcacaagga ttccaaagtg ttcctgtcat tacttctgat gcagaaacag ttatcggatt
     7381 ccgtcctgat cagttgcgta aacttgccag ctaacatgac taaaagaatc aaaacgtgat
     7441 acatggattt gaagggcttg agacagcacc agtttttgtc gcaaacttcc aaatcctttt
     7501 ttaaagctta aaaacaatgc aatgatttgc aatcaagcat catcaaatac gaaatgaagg
     7561 ttgggcgtac gtatgagtct taaaaattta caagatgtca gttatttcaa gctgaataat
     7621 gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata aggataaaga agctttagtt
     7681 gccttttttg aggaaaatgt caaaccaaat acgatgactt ttctatctat tatggaaaaa
     7741 atcaattact tgatcgatca agaatatata gaagcagaat tcattcaatt gtattcacca
     7801 tcatttattg aaaagctata tcaatttttg gacgagcaac atttttcttt caaatctttt
     7861 atggctgctt acaaatttta ttctcaatat gcgttgaaaa ccaacgacgg ttccgcttat
     7921 ctggaaactt atgaagatcg agtagccttc aatgcgcttt actttgcgaa cggggatgaa
     7981 gaattagcac ttgctcttgc agacgaaatg atccatcaac gatatcagcc agctacacct
     8041 tctttcttga atgctggacg taaacgtcgg ggcgaattgg tgtcttgttt cttgactcaa
     8101 gtcactgacg atatgaactc tatcgggcgc tcaatcaact ctgctttgca attgtcccgt
     8161 attggtggcg gcgtagggat tactttatca aatcttcgtg aagccggcgc tcctatcaaa
     8221 ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga aattattcga agatagtttc
     8281 agctattcta atcaattagg tcagcgtcaa ggagctggcg tggtttattt gaacgttttc
     8341 catccagaca ttattgcttt cctttcagca aaaaaagaaa atgcagatga aaagattcgc
     8401 gtaaaaactc tttctcttgg cgtgatcgta cctgataaat tttatgaatt gacacgtaaa
     8461 aacgaagata tgtatttatt tagtccatat agtgtcgaaa aagaatatgg tgtcccattc
     8521 tcttatgtaa atattacagc tgagtatgat catctagttg caaatccaaa cattcgcaaa
     8581 caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa aattacaaca agaatctggc
     8641 tatccttacg taatcaatat tgatacagcg aaccgcgaga atccaatcga tggcaagatc
     8701 atcatgagta atttatgttc agagatttta caagttcaaa cgccttctgt gattaacggc
     8761 aaacaggaat atgaagtatt aggaacggat atctcatgta acttaggctc gacaaatatc
     8821 gtgaatttga tgcaaagccc taactttggt caatctgtgc gtacgatgac tcgtgcattg
     8881 acatttatta cagatgcatc tgatatcgat gtcgtaccac cgatccaaaa cggaaataag
     8941 ctgaatcata cgattggatt aggtgccatg ggcttacaca cttatcttgc aaaagaacaa
     9001 atggaatatg gctcagagga ttcattagac tttacaaata tttacttcat gctgttgaat
     9061 tattggacac ttgttgaaag caacaatatc gctcgtgaaa gaaaacaagt tttccataat
     9121 tttgaaaaat cagcttatgc tgacggcact tatttcgaaa aatacgtaac tggagaattc
     9181 cagccaaaat cagaaaaagt caaagcattg tttgaaggca tttttatccc aactgctgaa
     9241 gattggaatg ctttgaaaca agcagtaatg aaagatggac tgtatcatca aaatcgcttg
     9301 gcagtagcac caaatggctc aatttcatat atcaacgata cgagtgcaag tattcatcct
     9361 atcactcgca tgatcgaaga acgccaagag aagaaaatcg gtaaaattta ttacccagcg
     9421 ccttatctat ccaatgagac gattccgtat tacacttctg cttatgatat ggacatgcgt
     9481 aaagtaatcg atgtttatgc aatggcacaa caacacgtcg atcaaggcat gagtttgact
     9541 ttattcatgc gttctgaaat cccagaaggc atttatgaat ggaagacaac aacgaagcaa
     9601 acgacacgtg atttgaatat tttaagacat tacgctttcc acaaaggaat caaatccatt
     9661 tattacgtac gtacctttac tgatgatgca gaagaaatcg gaagcaatca atgtgaaagc
     9721 tgcgtgatct aatatcatag atagattcgc ttattcatat tttaattaaa ggagcattct
     9781 catgtcagaa acatattatg aagcaataaa ctggaatgaa attgaagata tcattgataa
     9841 atccacttgg gagaagctga cagagcaatt ttggctagat acacgtatcc cactttcaaa
     9901 tgacctagat gattggcgta cattgtctca attggaaaaa gatacagtag gacatgtatt
     9961 tggcggtttg acactgctag atactgtcca atctgaaagt ggcatggatc agttgcgtaa
    10021 agatgtccgc actccccatg aagaagccgt tttgaacaat attcaattca tggaatccgt
    10081 tcatgccaaa agttattctt ctatttttag tacactgaat acgaaaaaag aaatcgaaga
    10141 aatttttgac tggacaaata cgaatcctta tctgcaaaaa aaagctgagc gtatcaatga
    10201 aatctacaaa cacggtacac cgctagaaaa gaaaattgcc agcgtctttt tagaaacatt
    10261 cttgttctat tcaggttttt atactcctct ttattattta ggaaacaaca aattggctaa
    10321 cgtagcagag atcatcaaac taatcatccg agatgaatct gtacatggaa cctacatcgg
    10381 ttacaaattc caattaggat tcaacgagtt acctgaagca gaacaagaaa cgttaaaaga
    10441 ctggatgtac aatcttcttt atgagcttta cgaaaacgaa gaacgttata cagaagaatt
    10501 atacgatcca atcggctgga cagaagaagt caagacattc ctacgctata atgccaacaa
    10561 agcattgatg aacctaggga tggatccact ctttccagat actgccaatg atgtcaaccc
    10621 aatcgtaatg aacgggattt caacaggcac aagcaaccat gacttcttct cacaagtcgg
    10681 aaacggctat ttgttaggtc atgtagaagc gatgaaagac gacgattatt tgattggatt
    10741 ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa taaaaagagt ttagcgtaca
    10801 gatattttaa atgtacactg aactcttttt tatgcttatt cacttgtttc ttccagcact
    10861 tttggactgt ctaacttttc cgaaacatgt tccattcttc tcaataaata gattttccgg
    10921 atatttaata ttatcgtctt aatcactgca tagacaggta tgcaaatcag catgcctaat
    10981 attcctgcga cacttcctgc tccaatcaat accaagatga tcgtcaaagg atggatattc
    11041 attgatttcc caaataacaa cggcttgatc aaatttccat ctaattgttg gatcactaaa
    11101 atcgacaacg ccatatacaa agcttgaaat ggagagataa acaaaccgac aatgattgct
    11161 ggtaccgctc caataaaagg gccgacatac gggataatat tcgtgattcc gcaaatgacc
    11221 gcaagcaaca agctataagg ctgatcgaat attctcattg ccaaaaagct cattacacca
    11281 ataatcaaag catctaagat cgtactgctg atataggcag ataatgtttc gtttaattct
    11341 tttaccgttt ggcgtaactc tttacgaatc gctttcggaa agaagtgaga caacgcatca
    11401 ataaatttgt gcccatcctt gaacataaat agcaaaataa atggaacggt aaataacaaa
    11461 ataaaaaatt gcatcataaa tgaaatgatt tttgagacac ttgcagataa actgactaat
    11521 atgatattga acaaattact aagagataaa tttgctgatg ctaattgttc ttccaaattg
    11581 aaatttttaa actcttcccg ctgcgccaat tcatttaacc atttgcttgt ttcatctgca
    11641 tacattggca agctctgggt caattgaatt gtctgttcca ttaactgagg cactacattc
    11701 atgacagtta aaacaactaa aatgattacg accaaaaacg tcaataaaaa accgaacact
    11761 ctcgggatcc gatgcttttc gagaaaaaca acgattggat cgaacatata atagataaaa
    11821 ccagcaacaa gcaatggtaa aaaaactgcc gaaagcattt tcaatattgg actaaaaata
    11881 ttcggcattt gtaaaataaa gtacacgccg atgatcgtga gtactagctc cactgtccag
    11941 aaaaataatt ttgattgttt aaaacgtgat tgcatacatc tcctccaagg tcctttgata
    12001 cttctattct atcatttaac aaagagttac gcgctgagat tttcatttgc ttaaaaaaaa
    12061 caaaaaaaat aaacatattt taatgagtag acgagtactt aaacaaaaac acggtttgga
    12121 gtagtgacaa aaattatact gcagtccctt tttccaaata aacggtgttc aaaagcttgc
    12181 atcttcgtaa taagcccagc caaacgaaaa atataaggaa ctatttttat ttggctgggc
    12241 ttattacttg atgtaggaca tctattttac aacctcagat catttattgc gcaatactga
    12301 ctaaatgcaa ctttgtgata tttttgtgta cggacaactc ctcgatcaca tcggcataag
    12361 tgagcccttt tggaagatca atcgtataga tatttttgta cactcggtag tcatcaacga
    12421 attttacgtc aaaattcaca tcttcgatta cgatattttt ctcttcaaaa taatcggtaa
    12481 gaaaatcttt ggtttctttt ctatggataa attggatttc taacttttta gtcgtaggta
    12541 catggatcac gcgtttcact actgtgagtg caatagcaat cccgataaaa ctgatgatcg
    12601 caatggaata gtaacccatg ccgatggcga tccctaatcc agcaattgcc cataaagaag
    12661 cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat gatcgttccc gcgcctaaga
    12721 aaccgacacc actcaccact tgagcaatca gccttgcttc atctgaacga atcgttccag
    12781 caagctgtgg atactctaaa gcatctctta atgcaccact agcgatctca acttggatca
    12841 atgcaatgat acatgctccc atacaaacta aaatatgcgt acgcatacct gctggacgat
    12901 ttttatattg tcgttcaaaa ccgattgctc cgccaaatag catagcgatc cctaaacgta
    12961 aaataatctc tggaattgtc aataccatat cattcatcta aacaccctcc agtccaatat
    13021 atcatgtctg aaaggagcag acaaaaaaag tgcaattttt acctcttacg aaaaaaggac
    13081 tgtgacaaaa tttttgtcat agtccttttt atgaataaac ggtgttcaat cagctgaccc
    13141 tcggtattac tcaacaaacg gcaaaatatg gggagctatt tttcctttgt cttctctaat
    13201 actcaggtca aaacgctgat ttcacaacct ctttagccta ctattgaaaa ttacaactta
    13261 cttgctgctt cttcatcgat gatcacaaca acattgtcgt gattttgcaa tacacttgct
    13321 ggcaaatgat tcgttacagg tccttcaatc atccctttga ttgcatcagc tttgctttcg
    13381 ccaaatgcca tcaagacgat ttctttactt ttcataattg aaccgattcc cattgaaaca
    13441 gcttttgttg gtacatcttc tgctttatcg aagaatcgtt tgtttgcatt gattgttgat
    13501 tctgttaagt caactactga agtcgttccg tcaaatgaag caccaggttc attgaaaccg
    13561 atatgtccat tttgcccgat tcctaagatt tggatatcga ttggatgtgc atcgatgatt
    13621 tgatcatact ttttgcatgc tgcttccaga tcttctgctt ttccatctgg tacaaacgtt
    13681 tctttgaatg gttttttatc aaataattgg acatccatga aatgacggta gctttgttca
    13741 tctgaaccgc ctagtccaac gtactcatct aagtttacag aagtcatttc actgaaatcc
    13801 aaatcgcttg aagtcatttc tttatataaa gtaactggtg tgcttcctgt tgccaatcct
    13861 aatacttttg cacctttgtt cataccttct ttgatgattt caaatgcttt ttttccgcct
    13921 tcttcagcgt tttttacacg aatgatttcc atagtgattg ctcctttata ttttggtata
    13981 gcccaattat agcattcttt ttttcttttt acaataatta gtatagacca aattgcattt
    14041 ttttcatttt cttgacaata aataggaatc cgtgttaggt ttcataagat ggttcaatca
    14101 aacaatccat gttgcatgaa aaccaagcaa gtgattcact gtggacgctt tatgtctgca
    14161 gtttttttat gaaaaaggag ttattatgag tatactgaca ttaaacaaca ttacccagca
    14221 atttggcgac aaaatcctct atgaaggtgt cgatctgcaa ttaaatgctg gcgaacatct
    14281 tgggctgatt ggacaaaacg gagccggtaa aagtacattg attaaaatca tcactggtga
    14341 gatcttacca gatgacggac agatccattg gcaaaaaaat attcacaaag gttacctcga
    14401 tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa tttctaaaaa ctgcgtacgc
    14461 aaaagaatac gaaaaggaag aaaaaattgc ttttctttac caaacatata gcgagacctt
    14521 cgatgattcc ttgctggaaa aagctgggaa actccaaacg gaattagacc aaggagtgtt
    14581 ttatcaaatc gacaccttag ttgctgagat gagcagcggg ttggggatcg atgtcttagg
    14641 tttagattca ctgttgaaaa atctaagtgg tggacaacgt tcaaaggtga ttttagcaaa
    14701 actactttta gaaaacccag atgttctgat cttagatgaa ccaaccaatc acttagatga
    14761 tcagcatatc caatggctga cgcagtttct tcaggatttt gacggtgcgt atatcgtgat
    14821 ttcccatgac caagaatttc ttgatcagat caccacccat atcgcagaca tcgagtttgg
    14881 aaagattact aagtatactg gccatctaaa acaagcattg aaacaaaagg aacaaaatcg
    14941 cgaaagctat ttgcgccaat atcatgctca acaaaaacaa atcgaaaaga ccgaagctta
    15001 tattcgaaaa tacaaagccg gttcccgatc aacgatggca aaaagccgac aaaaacaatt
    15061 agataaaatc gaacggctga cgcctccagc ttcagcaagt aaagctgtct ttgattttcc
    15121 attctcgcct attgtcacga cattagcagt ggaaacaact gatttagtca tcggatatga
    15181 tcgaccgcta cttgcaccca tcaatttgac catccgcttt ggtgaaaata ttgccattcg
    15241 aggcttcaac gggattggaa aatctacttt attaaaaaca ctgatcggtg agattgaaaa
    15301 aataagtggc gatttccact tcccagataa cacaaaaatc aattactttt cacaagatct
    15361 gtactgggaa aatccattag aaacaccact acaatattta agcagtcaat ttccaaaagc
    15421 gactatcaaa gaattacgtc gccaactagc taaagcaggg ttagttaatc aattagcaag
    15481 tgaacctctc actacattaa gtggtgggga acaaactaag gtgaaacttg cacagttgac
    15541 aatgaaccaa ggtaatttac tgattctcga tgaaccgacg aaccatatcg atcaagaaac
    15601 gaaagaaagc ttacaagacg ggatacagaa atttccagga acagtaatca tcgtctctca
    15661 tgaacaagaa ttttatcaag atcttgtgca acgagtcatt gagattgaac caaaataaac
    15721 gtttgattga atagttgaaa aaaaggagct gtgacaaaag ttttgtcaca actccttctt
    15781 ctgaataaat ggtggccaat tagctgacct tcggtattag ctcaaaaaac ggcaaaatat
    15841 gaggagctat ttttatttgg ctattcttat tacttgatgc agaacagctt ttcacaacct
    15901 cttttcgtct attttttatt ttcgagattt ctctttcctt taaaaacaac tgtttgaaag
    15961 aagaacgcac atccttgaaa aacaagcccg ctaaccgtta cacctaacca gccatactgc
    16021 tgccacattg tcgtccctag caatgaacca gccgcacctc caataaagta cataaacata
    16081 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
    16141 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
    16201 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
    16261 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
    16321 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
    16381 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
    16441 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
    16501 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
    16561 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
    16621 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
    16681 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
    16741 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
    16801 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
    16861 gccattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
    16921 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
    16981 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
    17041 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg ttttttttca
    17101 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
    17161 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
    17221 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
    17281 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
    17341 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
    17401 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
    17461 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
    17521 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
    17581 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
    17641 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
    17701 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
    17761 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
    17821 actaccagta tcagaatgat tcccaacgag atcaatatca atcggattat tttctttttc
    17881 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
    17941 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
    18001 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
    18061 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
    18121 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
    18181 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
    18241 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
    18301 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
    18361 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
    18421 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
    18481 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
    18541 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
    18601 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
    18661 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
    18721 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
    18781 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
    18841 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
    18901 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
    18961 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
    19021 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
    19081 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
    19141 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
    19201 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
    19261 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
    19321 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
    19381 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
    19441 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
    19501 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
    19561 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
    19621 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
    19681 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
    19741 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
    19801 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
    19861 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
    19921 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
    19981 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
    20041 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
    20101 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
    20161 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
    20221 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
    20281 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
    20341 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
    20401 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
    20461 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
    20521 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
    20581 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
    20641 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
    20701 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
    20761 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
    20821 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
    20881 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
    20941 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
    21001 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
    21061 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
    21121 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
    21181 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
    21241 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
    21301 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
    21361 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
    21421 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
    21481 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
    21541 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
    21601 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
    21661 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
    21721 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
    21781 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
    21841 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
    21901 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
    21961 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
    22021 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
    22081 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
    22141 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
    22201 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
    22261 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
    22321 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
    22381 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
    22441 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
    22501 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
    22561 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
    22621 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
    22681 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
    22741 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
    22801 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
    22861 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
    22921 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
    22981 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
    23041 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
    23101 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
    23161 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
    23221 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
    23281 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
    23341 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
    23401 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
    23461 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
    23521 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
    23581 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
    23641 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
    23701 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
    23761 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
    23821 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
    23881 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
    23941 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
    24001 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
    24061 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
    24121 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
    24181 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
    24241 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
    24301 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
    24361 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
    24421 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
    24481 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
    24541 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
    24601 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
    24661 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
    24721 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
    24781 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
    24841 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
    24901 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
    24961 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
    25021 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
    25081 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
    25141 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
    25201 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
    25261 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
    25321 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
    25381 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
    25441 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
    25501 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
    25561 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
    25621 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
    25681 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
    25741 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
    25801 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
    25861 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
    25921 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
    25981 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
    26041 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    26101 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    26161 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    26221 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    26281 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    26341 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    26401 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    26461 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    26521 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    26581 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    26641 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    26701 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    26761 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    26821 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    26881 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    26941 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    27001 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    27061 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    27121 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    27181 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    27241 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    27301 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    27361 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    27421 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    27481 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    27541 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    27601 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    27661 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    27721 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    27781 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    27841 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    27901 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    27961 gatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    28021 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    28081 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    28141 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    28201 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    28261 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    28321 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    28381 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    28441 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    28501 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    28561 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    28621 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    28681 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    28741 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    28801 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    28861 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    28921 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    28981 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    29041 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    29101 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    29161 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    29221 tggtttcgtt tactgatctc tttgcgatta aatgctagat tgatgacgct gaaaccgcta
    29281 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    29341 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    29401 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    29461 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    29521 tgtatcttga tcgtcggcat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    29581 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    29641 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    29701 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    29761 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    29821 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    29881 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    29941 attggtataa aaactagcca aatcagtgta gaaataaagt tt
//
LOCUS       DACDTH010000031        27942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDTH010000031 DACDTH010000000
VERSION     DACDTH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.031"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWK54_001488"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWK54_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9593613.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWK54_001489"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWK54_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9593614.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWK54_001490"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWK54_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9593615.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1642..2274
                     /locus_tag="IWK54_001491"
     CDS             1642..2274
                     /locus_tag="IWK54_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9593616.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="IWK54_001492"
     CDS             complement(2276..3181)
                     /locus_tag="IWK54_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9593617.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3919)
                     /locus_tag="IWK54_001493"
     CDS             complement(3266..3919)
                     /locus_tag="IWK54_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9593618.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="IWK54_001494"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="IWK54_001494"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9593619.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="IWK54_001495"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="IWK54_001495"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9593620.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="IWK54_001496"
     CDS             6168..7253
                     /locus_tag="IWK54_001496"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9593621.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="IWK54_001497"
     CDS             7250..8314
                     /locus_tag="IWK54_001497"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9593622.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="IWK54_001498"
     CDS             complement(8563..9366)
                     /locus_tag="IWK54_001498"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9593623.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWK54_001499"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWK54_001499"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP9593624.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="IWK54_001500"
     CDS             complement(10244..11251)
                     /locus_tag="IWK54_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9593625.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="IWK54_001501"
     CDS             complement(11258..11902)
                     /locus_tag="IWK54_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9593626.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="IWK54_001502"
     CDS             complement(12002..12760)
                     /locus_tag="IWK54_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP9593627.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWK54_001503"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWK54_001503"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP9593628.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWK54_001504"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWK54_001504"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP9593629.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWK54_001505"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWK54_001505"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP9593630.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWK54_001506"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWK54_001506"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP9593631.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWK54_001507"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWK54_001507"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP9593632.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="IWK54_001508"
     CDS             18158..19027
                     /locus_tag="IWK54_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9593633.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="IWK54_001509"
     CDS             complement(19153..20052)
                     /locus_tag="IWK54_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9593634.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="IWK54_001510"
     CDS             complement(20217..21041)
                     /locus_tag="IWK54_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9593635.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="IWK54_001511"
     CDS             complement(21052..22347)
                     /locus_tag="IWK54_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP9593636.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="IWK54_001512"
     CDS             complement(22363..22842)
                     /locus_tag="IWK54_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9593637.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="IWK54_001513"
     CDS             complement(22848..23876)
                     /locus_tag="IWK54_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP9593638.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="IWK54_001514"
     CDS             complement(23893..24999)
                     /locus_tag="IWK54_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9593639.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IWK54_001515"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IWK54_001515"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9593640.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         7879 a         6131 c         5040 g         8892 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa ta
//
LOCUS       DACDTH010000032        27517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDTH010000032 DACDTH010000000
VERSION     DACDTH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.032"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..648
                     /locus_tag="IWK54_001516"
     CDS             1..648
                     /locus_tag="IWK54_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9593641.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            732..1676
                     /locus_tag="IWK54_001517"
     CDS             732..1676
                     /locus_tag="IWK54_001517"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9593642.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1811..3262
                     /locus_tag="IWK54_001518"
     CDS             1811..3262
                     /locus_tag="IWK54_001518"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9593643.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3283..4119
                     /locus_tag="IWK54_001519"
     CDS             3283..4119
                     /locus_tag="IWK54_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9593644.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4119..4778
                     /locus_tag="IWK54_001520"
     CDS             4119..4778
                     /locus_tag="IWK54_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9593645.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4904..5593
                     /locus_tag="IWK54_001521"
     CDS             4904..5593
                     /locus_tag="IWK54_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593646.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5599..6555
                     /locus_tag="IWK54_001522"
     CDS             5599..6555
                     /locus_tag="IWK54_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9593647.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6599..7111
                     /locus_tag="IWK54_001523"
     CDS             6599..7111
                     /locus_tag="IWK54_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9593648.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7367..7915)
                     /locus_tag="IWK54_001524"
     CDS             complement(7367..7915)
                     /locus_tag="IWK54_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9593649.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8004..9185)
                     /locus_tag="IWK54_001525"
     CDS             complement(8004..9185)
                     /locus_tag="IWK54_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9593650.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9421..10929)
                     /locus_tag="IWK54_001526"
     CDS             complement(9421..10929)
                     /locus_tag="IWK54_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9593651.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11088..11279
                     /locus_tag="IWK54_001527"
     CDS             11088..11279
                     /locus_tag="IWK54_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593652.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11482..12399
                     /locus_tag="IWK54_001528"
     CDS             11482..12399
                     /locus_tag="IWK54_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9593653.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12392..13465
                     /locus_tag="IWK54_001529"
     CDS             12392..13465
                     /locus_tag="IWK54_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593654.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13613..14131
                     /locus_tag="IWK54_001530"
     CDS             13613..14131
                     /locus_tag="IWK54_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593655.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14112..14504
                     /locus_tag="IWK54_001531"
     CDS             14112..14504
                     /locus_tag="IWK54_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9593656.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14580..15515
                     /locus_tag="IWK54_001532"
     CDS             14580..15515
                     /locus_tag="IWK54_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9593657.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15648..16427
                     /locus_tag="IWK54_001533"
     CDS             15648..16427
                     /locus_tag="IWK54_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9593658.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16420..16737
                     /locus_tag="IWK54_001534"
     CDS             16420..16737
                     /locus_tag="IWK54_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9593659.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            16981..18591
                     /locus_tag="IWK54_001535"
     CDS             16981..18591
                     /locus_tag="IWK54_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9593660.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18748..19596
                     /locus_tag="IWK54_001536"
     CDS             18748..19596
                     /locus_tag="IWK54_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9593661.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19827..20306
                     /locus_tag="IWK54_001537"
     CDS             19827..20306
                     /locus_tag="IWK54_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593662.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20339..22567
                     /locus_tag="IWK54_001538"
     CDS             20339..22567
                     /locus_tag="IWK54_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9593663.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22696..24339
                     /locus_tag="IWK54_001539"
     CDS             22696..24339
                     /locus_tag="IWK54_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9593664.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24412..24957)
                     /locus_tag="IWK54_001540"
     CDS             complement(24412..24957)
                     /locus_tag="IWK54_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9593665.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(24980..25465)
                     /locus_tag="IWK54_001541"
     CDS             complement(24980..25465)
                     /locus_tag="IWK54_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9593666.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25455..26267)
                     /locus_tag="IWK54_001542"
     CDS             complement(25455..26267)
                     /locus_tag="IWK54_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9593667.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26406..>27517)
                     /locus_tag="IWK54_001543"
     CDS             complement(26406..>27517)
                     /locus_tag="IWK54_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9593668.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9010 a         5017 c         5512 g         7978 t
ORIGIN      
        1 atgaaaaaaa ctgttattta tactagttta tttgcagctt tgtttttagc tgcttgtcaa
       61 tcaaatacca atcaaggcac atctgggaat acgtcttcca gtagcgaaca gcgctcttca
      121 caggtatcgg actcctcaac aaaagcaagc acatcttcgt ctagccagac aaaaagttct
      181 agtgaagcaa gtactcagtc agatatcaaa gtgtctgcag ctcgtgcgat cgaattgttc
      241 caagataaat acccagatac tgcaattact agtttggaat tagatagtga ttggggatct
      301 tacttctaca agatagaagg tgtcgatgat caaaaagaat acgaagcaag aattgatgct
      361 caaaatgaaa aaatggagac agagaatcca gaaactctag atcgagatga acaaaatggt
      421 caaaagaaaa aagaagatgg tctggatgtt gataaattga tcacgattga ggaagcagca
      481 aaaatcgccg tcgacaaagt aggaagcggt acggcaacag attgggattt ggataaagat
      541 ttaggtacta cttattggga agtcaaagta aaagacggca atcaaagtac atcagtcaag
      601 atcaatagcc aaacaggcga agtactgtca actgaacaag atgattaaga atttcttcgg
      661 aaaacaagag ttgatttttc ttttagtcat gatacactag acaaaataac ttgtttgaag
      721 gagcgattta cttgagttta gaaacgacct ttgccaatca tatctttgcc aatcctttga
      781 tgaatgcatc tggtgtccac tgcatgacta cccaggaatt agatgaactg gctcattcag
      841 aagcaggagc atttatcact aagagctgta cgatcaatga acgaaaagga aatcccgaac
      901 cacgctattt tgatgttcct ttagggagta tcaattctat gggacttcca aacttaggtt
      961 tttcttatta tctagagtat gctcttgcgt atgaaaaagt gcaggaaaac caaaatcaac
     1021 cgttattttt ttccattgcc gggatgagtg tccaggaaaa cttggaaatg cttgagaaaa
     1081 tagaaaaaag tgggtttaat gggatcactg aattaaattt atcttgtccc aatgtgcctg
     1141 gaaaaccaca actagcttac gattttgagg ccacttatga aacactgaaa gaagtattca
     1201 gcatcttttc taagccgcta ggaatcaaat tgcctcctta ttttgatttt gctcattttg
     1261 atcagatggc tgatatcttg aatcagtttc cattgaccta tgtcaatgct atcaacagtg
     1321 tgggcaatgg tttgtatatc gatacagaac aagaagcagt agtcatcaaa ccaaaagaag
     1381 ggttcggcgg cattggcgga gagtatatca agccgactgc tttggcgaat gttcgcgctt
     1441 tttatacacg tttgaaacca gaaatccaaa tcattggtac aggtgggatt cggacaggac
     1501 aagatgcatt tgaacatctg ctatgtggcg caagcatgtt acagattggt accgagcttc
     1561 ataaagaagg gccggaaatt ttttctagaa tcataaaaga attaacgcag attatgtcag
     1621 aaaaaggata tacgtcaatt gacgaattta aaggcaaatt acgcaccatt tcatagcaaa
     1681 gagacgaagg tcatgattga tcttcgtctt tttttataaa aaacactcgt ctgttcattc
     1741 ctgtgcaagc gtactcattg tatgttagac gaacttgtgg gaaaataaca gaggcaaaaa
     1801 ggaggataat atgaatgatg caacagaatt agcaaaagag cttaaagaaa aagaatttag
     1861 ttttcaagaa ctagttacgg atatacagaa aaaaataaag aagcaaaatc atgaattgaa
     1921 tgcgtttgtc gcgcttgaac cattcgagag taattcccac gagctattga ttccccagtc
     1981 agaaaaaggg ccattggctg gtatcccatt tcctttaaaa atgctcggtc aagaaaaaaa
     2041 gggatggcta gctacttcag gatctcgttt atttgaaaca caccgagctt ctcatacatc
     2101 taattatgtg cggcaagcag aagcgatagg tctagttcct tttggacaaa ccaatgcgcc
     2161 agagtttggt ttcaaaaata ttacagaccc agtcatctat ggcccagcaa gaaacccttg
     2221 gaaccttgaa tgtacaccgg gcggatcaag tggaggagct gctgctgcag tttcatcagg
     2281 aatcgttcct ttagctggtg ctagtgatgg cggtggttct attagaatac ccgcttcgtt
     2341 ttgcggattg attgggctga aaccttcgcg agggacgatg ccagttggtc catatgcttg
     2401 gagaggatgg caaggagctg ctattgattt cggcttgaca gtttctatga gagacacgga
     2461 ggctttattt tatgggatga gaagtatcca ttccggtgcg ccatatcaag tatcgccagt
     2521 tgagtggcaa acacatccaa gaaaaaaacg gttgaagata gctttttgtg ttgattcacc
     2581 gatccactca cagattagtg aagaggcagt acttgctgta aaaaaagctg tagatttcct
     2641 tggaaaagaa gggcatgatt tgacagagat tccttaccca ctagatggtc gtaaactgat
     2701 cacgtcttat tatcagatga atggcgcaga aacagcagca atgatccgct cgattgaaac
     2761 aggcatccag cgagctgtca gaaaagaaga acttgaaccc atttcttgga ctttgttcca
     2821 atatggtcag aagctctctg ccgctactta catcgattct ctgcatgtat gggatgaagc
     2881 agcaattgtc atggaaaacc tacatcaaga atacgatcta ttcctttcac caacgactgc
     2941 atttaccgcc cctaaaatca gcgaagattt gcaaagtgat gcgataagag cacagatgga
     3001 acacatcagt gattgttctg aagtagaagg tttagacgtt gtttcttcca tgtttgagga
     3061 gagcttgaag atcacacctt atacacagtt ggctaatctg actggacaac cagcaatcag
     3121 cttgcctact catttgacaa aagaaaacat gcctctagga atccaattta tggctgccag
     3181 aggaagagaa gatttgctat ttcaaatcgg ctatctattt gaagcaaatc atcagttcca
     3241 tttaccaaat ccatggaaat agcagaaaca aggagaagaa gaatgaagaa aaaaagattg
     3301 tttgctctaa taactagtgc cttttttatt ttaagcgctt gcggtacagc tgccgctaat
     3361 caggaagata acagctggag taatgtagaa aaaaagaaag aattagtagt agcgacttct
     3421 ggcacactgt ttcccgcatc ttattataat gatcaaaatg aattagttgg ttatgatgta
     3481 gatttggcaa aagaggtggc taaacggtta aatgtcaaaa tctcctttaa agaatacaac
     3541 gtggatggac aaatcacttc tgtttcaaaa agagaatcag attttgctgc gaacgatttt
     3601 ggtatcacaa aagaaagaga aaaaaaattc tccctttcag agactttcaa atattcattt
     3661 gacagtatga tcgtacgaaa aaaagatgat tcaggaatcc actcacttga agatcttaaa
     3721 gggaaaaaag cagctggcga acccaatact agctatatgc gtttagcgga aaaacacgga
     3781 gctaaattag tcacatatga caatgccacg aatgatcagt atttaagcga tgtagcaaat
     3841 ggtcgaacag atgttattct aaatgattat tatctgcaga aaatggccgt atctgcttta
     3901 ccagatatcc ctgtaaaaat actggagaac gtttatttca atgaaaacga aagtggtttt
     3961 ttattgaaag aaggaaatga tgctctgaaa aagaaaatcg atggcgtatt aaaagagatg
     4021 aaagaagatg ggacgacaaa gaaaatatct gaaaaatatt ttcaaactga tgtttcagtc
     4081 aaaccgaaag aaacgatggc tgagtcggtg agtgagtgat gtactttcct ctaattgatt
     4141 tttccctcat gtggacttca cttcctttcg tactgcaagg cttggtatac acattaggga
     4201 ttggcttcgt gtcgtttctt ttaggaaatc tggtagggtt gcttttgaca gttttaggtc
     4261 ttttggattg gctgcctctg aatgtattta ttcgttttta tttatcgttc tttagaggca
     4321 ttcccgcact agtcctgctg tttctgttat attttgggtt accttatcaa ttaagtgcct
     4381 taacggcttc agtcatctgc tttacgatta ccagcagtgc atttattggt gaaatctatc
     4441 gaggatcact agcaggcgtt tcttcaggtc agtgggacgc tggtttagct tctggttttt
     4501 ctttctttgg ggtattgaaa tatatcgttt tgccgcaagc ttttcgaatc tcagtaccgg
     4561 cactcagtaa cgtagctatg gatctagtga aaggtacttc gttagcagca acaatcacag
     4621 tgccagaaat atttcaaaaa gcaaagatca taggcggtcg gacattcgat tatttttctc
     4681 tctatatttt agttgcactg atttattggg tcatttgtat cgggatcgaa aaagtgcaac
     4741 agtatttaga aaaacgttct atgcatcagt caatctgaaa aatagaaatg gtgctagcgt
     4801 ggctggcacc atttctattt tttgtttctt gtatattctg cctagtattt taaaaccatt
     4861 tcatgtaaaa tgtaaatcga aaatatgttc ggaggtagaa aacatgaaga ccccgctaac
     4921 catcgaaata gaatcctcgt tataccaata ttgcatcgag caaggcggct tggttgtaga
     4981 agaagtcacg atgcctgatg accaaggaat tgtagatacg cttagctgct tgacaaaatt
     5041 tgatggaagc cgagaatggc gatgctatga gctgaaagtg acaaaagcag attttcgctc
     5101 tacagccaaa ctgtcatttg ttggacatta taattatttt gttttaccaa gtaaactata
     5161 tgaagaagta aaaactgaaa tccctacagg aataggtgtt ttagtgtatc gtccgtacac
     5221 ggttgaagag gagatgcctg cctctggtac atttctgatt gcgaaaaaag cagcaaagaa
     5281 agagcttgct gtaccggaag aagctttgac aaaccgcttc atggcttctt tattcaggga
     5341 agtaagaaaa gcaaagcaaa tggagcgagg cgtaaaatat tttccaacag atcaattgta
     5401 caaagaattg aaaagacgtt cagaaaatca agatccattt tctaacgaac gatattatga
     5461 acggttttta gaggaaatag ctagtcaggc aatcaatgag ttgcaagaag aagttatcgc
     5521 gcttcagcat gattatgaat atttgaggca acaacagcag atccgtcggt tgcctacaga
     5581 gccattggag tgaaaaatat gtactatcct tatttaagag gaaaacaatt tgacctatta
     5641 gcattaaaag aagcactgtc gcgaggattg ctctccaata aaatccaacc agtgatcgaa
     5701 ccagtacgtg attccgctac gcttaaaaat gtgatcgaat tatttcaaaa aaagcaccat
     5761 ccaatcgctg tgatccaaaa tccccaagta ggacaattca aattattcga tcagcatgtg
     5821 cacagttgga cagtcaaaga aaatagctct gtcgtccctg cacagatctt aacgccagaa
     5881 aatctcagtg aagtactgga ttcacctcca gcgtttcttg ttttcgatgg acagcactac
     5941 ccaaaagata cagaggtgtg gaaacagttg gcaggagttg attcgaaatt tttgatacca
     6001 gatactagtc gctttcgcat ctggctgccc gaaaacaaaa ttgtgatccg tgactctttt
     6061 caaacgagaa agcatgttga aagttatgca gataaaacag atgacttttt tagtgatgac
     6121 tatctttttt tccacgcaga cggatatatt ggattttctg atttcacgat cgaaggtagc
     6181 cgttatttcg ataaagggtt ccctagccga gcgcttgcta tccatctaac gtatattgat
     6241 gcttacggca atatacgcgt gaaacatttt gtctctgaca gtaacgactc tgctaaagac
     6301 caagcattga agtttcttga agcaggagat aaaatgaaaa aatggatcat gcgtcaccat
     6361 catcaactct tgattacttc aggaatgatt gaattattgg ctctttacca gcaacaaaaa
     6421 ttccctggac tgggagtgtt gaaaaagtgg tcacttatgc atcatttgga acttattagt
     6481 cacctgctag atcatccaaa agattggctt aaatattatt caaaagttca ggaactatat
     6541 gaagtgatcc aatgattaga taatgtaaat gaaaaaggaa tagaaaagag gaaactggat
     6601 gtatattcgg caaacaagga aagaagaagt agaagaagtg gtagaaatca ttaatgccgc
     6661 agcggatttc attgcttcgc aaggctctcc tcaatggcaa aacgggcaga aaccaacaaa
     6721 agaaacgatt ttgaacgata ttgaaagaaa agaaagctat gtattaatag tggaagatca
     6781 aattgctgga acagctgcat tagtctcaag cgtggatccc gtttacacaa aaatagatgg
     6841 tagttggaaa gaatcaactg gtccttattt atcgattcat cgtgtcgctt tagcagaaag
     6901 tactcgagga aaaaaactag caaaaccgtt cttagaacat ttgttgacga ttgcagccag
     6961 caaaaatatc caagatatcc gtatcgatac ttatccagct aatgaaccga tggaaaagac
     7021 catctattct gctggattca cttatcaagg gatgattgaa tttccttttg aacacggcga
     7081 aagaaaagca tatcaaataa tcatcaattg actataaaat catctgagca aagataaaga
     7141 cattaacact actgaaaaaa acaatcatat gatagccaag aggttgtaac agaagtggcc
     7201 agcttcaaaa aacaagaaag aatttccgaa aatgcttctc aaatttttgt ggaattttag
     7261 cgtattttac gaaatagctg ctttctgaaa ctcccttcat cgagaggtcg tgacatagct
     7321 tttgtcacga cctctcggct tatcctacga gtattattcg atacatttat atcaataatc
     7381 ctttttttat tttatctgca gcttcttgga acgctccatc gtttgacacg actaaaagtg
     7441 aggggatagc tttcaaacgt ttcaactgtg ctaaatcttc tacataaacc gccagtctgg
     7501 ctgaaatctg ttcagatgaa tctccacgtt ttttcaggcg cgattgcaat gtttctttag
     7561 agatcgtaat aaagatagga cggactaccg tcccgaaata ctcatataaa tgccaaaaac
     7621 catttgctgt caaaacggcg taacaatttc ctactgtcag tttcttttta atttctgctt
     7681 tactggaacc atatttatgt tgagcatatt ggtcccactc tgcaaactca tttcccttga
     7741 tcatctgatt gaatgtattg tcagaaacaa aataataatc ctcttgatct ttttccccag
     7801 gtcttggtga tcgattcgtg taagtaatga tcttttgttc cggctgaaaa atagcttctg
     7861 cgagtgaagt ttttccagaa ccagaaggac cgataaatac atagcaataa ttcattgtca
     7921 tttctccttt gatgattgaa caagtgaaaa agctttggaa aatatttcca aagcttttaa
     7981 aagtaacttt gcttttttgt atattatacc agcaagccag tcacaatagc acttaaaaag
     8041 ctgactaaag tcgctgttag caaaattttc aatccgtgtt ttgcgacaag atttcctttt
     8101 tcttcattta atcccttcat cgcaccagaa atgatcccga tagaagagaa gttggcaaat
     8161 gaaacgagga agacagatat aattgctgtt gtacgctcag taaaatggaa attaccattt
     8221 gctaattctg tcatagcaac aaattcattt gtaaccagtt tagtcgccat gatactaccg
     8281 gcatccactg cttctttcca tgggattccg ctgatgaagg caagaggggc aaagatgtaa
     8341 cctaggatct cttggaaagt gatgccgaat atcccgtgga agatagcatt gatcatggca
     8401 atcaaagcaa caaaaccgat aagcatagca gcaaccgtga tcgccacatg gaaaccatca
     8461 agaatgtatt ctcccaacat ttggaaaaat gtctgttctt tgttttcttc aatgatcagc
     8521 tcatcttctt tttcttctaa tacataggga tttacaattg aagccagaat ataaccgcca
     8581 aataagttca aaacaagggc agtgatcaca tatttagaat cgatcagtgc catataagat
     8641 ccgacgatcg acatagaaac tgtagacata gccgatacac tcatggtgaa caaacgtttc
     8701 tcagatagga agggtaattc cttttttatg gaaataaaaa cttccgattg tcctaaaata
     8761 gcagaagcta ctccattata agattccaat ttgcccatcc cgttgatttt acttaatcct
     8821 agaccaacag ctttcataaa taatggaaga atcttgatat agcgtagaat accgataata
     8881 gcagaaataa atacaattgg catcaatacg gaaagaaaga aaggtgcact tcctttattt
     8941 gctacaccgg caaaaacaaa gtcaacacct tgcccagcaa aagcaagaag atgatcaaac
     9001 atttttgcca atacagaaac aaccgctgta ccaaaagttg tacgtaataa tacgaaacca
     9061 agaacaagct gtaaaacgaa catcacaatt agtggtttca atttgattgc ttttttgtta
     9121 ctgctgatca aaaaagcaag ccctaatgtg acaaataaac caataatacc taataaataa
     9181 ctcaaataat cactcctatt ccaaattccg ttttatttta cctgttttgc aaataaaata
     9241 ctaatcttta gtgaaatttt cattactttt ttagagtgaa acgcattcat tttccattag
     9301 aaaataaagc gaacaaaata aaaggtttaa aaagtgtaac gtcttagtaa agtctataaa
     9361 aaaacgaaac tatcgattaa gctaatagtt tcgttttcaa catttataag taaacttttt
     9421 ctattggtaa tatagatttt cggaagggta gacaggtgcg caggtaatat cgattcctag
     9481 ttttttcaat gtttgttcat catttttatt gaccatcaca gtagagtggg cttgtgctcc
     9541 ctcaagttca gctagtttat cataagcaac ttgagcgatt ggattcgtta ctgcactgat
     9601 tgataaagcg atcaagactt cattgatgct tagtgcatcc aaagaggaat gaagtgtctt
     9661 tgatttcaat ccttgtattg tttcaagaac taatggtgac aataaaggaa tgtcatctgc
     9721 gatatgggct aaatgtttta ctgcatttaa gataactgcc gctgacgcat ccatcaatga
     9781 acttgtacgc ccggtaatga tgacttcatc tggtaattcg attgcaatca cagcagaagg
     9841 atcttttgta ggactttgcg cttgaatctt atctgcatag tcatgggcag ggactaccac
     9901 gtttcggtct tcaggacgta aaccgacttc ttccataatc agtttgattc gattaactgc
     9961 ttcttcgtcg atcaatcctt ttttgaagtc gcattctgtt tggaaataac gacggatgat
    10021 ttcttgttta gaggcttctt ggacaacggt atcatccacg atcccaaatc ctacacgatt
    10081 cacacccatg tcagtaggag attgataaac agattcttta ccagtaattt tttcaataat
    10141 acgtttgatt acaggaaatg tctcaacatc ccgattatag ttcacagcta ctttattgta
    10201 ggcatcaaag tggaaggaat cgatcatatt gacatctttt agatcgactg tggctgcttc
    10261 ataagcaatg ttcagcggat gtttcaacgg aacattccat actgggaaag tttcgaactt
    10321 ggagtaacca gctgcatgtc ctttttggct ttcatgatac agttggttca aggaagtagc
    10381 gagctttcca cttcctgggc caggagctgt cactacaacg attggtttag ttgtttcaat
    10441 atactcattt tttccaaaac catcttctga gacgattttt tctacgttga ctggatagtc
    10501 ttcgatttct gagtgtttat agaccttgat gtttcgtctt tctagtttat tgataaagac
    10561 tttcgtggca ggctgtccac tatagcgagt gataacgaca ctgttgatta ataagccata
    10621 accacgtaat tcatcgatta accgcaagat atccatatca taagtaatcc cataatcacc
    10681 acggatctta ttacgttcaa tatctccagc atagacgcaa atcagtattt cagcttgatc
    10741 tttcaatttt tgcagcaatt tgattttcgc atcttcttca aatcctggaa gtacacgttt
    10801 cgcatgcttg tcgtcaacta gcttcccacc aaattctaaa tataatttgt catagtggtt
    10861 cactcgttcc aaaatatacg ctgactgctc ttcaatatat ttttctgaat caaaaccaat
    10921 ttttttcact aggttccctc cttaagtact aaagcatttt ttcatttttt tgaggattcg
    10981 tcaactggaa gagcgaaatt cctcaaaaaa acaaagaaaa tggtttaaac caacttgtct
    11041 agatagttat tcattggtat gatagttttt gataaggaag gtgctacatg aaaaaaagac
    11101 agaaaaagaa aaacgcatat aaacactata tccgttcgat ttttaccggt tatgaaagaa
    11161 tgcttgaaga tcccgaattg gaacaattga cgtttactta tctaaacgaa gaaacgcagc
    11221 taactcgtga tgagcatcag cgcattcatt tcacaacaag agatctgcct agtaaataaa
    11281 aaataagata aaggtcatcg gattcgattc tgtcctatca gatggaaaga ctagttgatc
    11341 gaaaaaaagg ctgtaccaaa ttttggtgca gcctgtttta tggagcattg cccgctcttt
    11401 tttataacaa ttttttacag aaaatggtac tattgaagta ttaataagtg aaaaacagat
    11461 gagtaagagg aggcaagtag tatgaaagag ccagatacaa atgcaaatta tgagccagac
    11521 gaactagagg agacgaatgc ggatcaggat acaggacaag aatcgtcttc tttttacgat
    11581 atcaaagaaa aagcaagcat gaatctttcc caattattag aagacttaca ggcttttgaa
    11641 aaagctgtcc gcgaagaaga tatccctgag atctaccgtt tatacaatgg ccgattgaat
    11701 gaagaattaa aaagaagctc aaatgaaaat cacgagatcg atcaattgct gatgcgtaag
    11761 atccatgatc gttttttaca ggaatttcct tttatggaac aagtagaaaa catctcgcca
    11821 acgatgagct attacaaaat cggcacttat taccatgatc ggcccacggt cggtattgat
    11881 gcaagtctgc cagaaatttt tgttttgcct aagatcgatg aagagtggga gaaatattcc
    11941 caaccggagt caacggacta tgacaaaaaa atcaatgagc tagacgcaaa ggtgatcact
    12001 gctcagacag aaattgaacg tttagatgaa caaataaaag aaatcaatcg acaaatgact
    12061 gatttgaacg acaataaagg attcttgaat cgtaaaaaaa tcgaggaaga aattcaagaa
    12121 ttagagaaga agaagcaagt actatctaat gaaaaattag gctggcttcc ttatattgag
    12181 acgcctgaaa caatccagca gcaaaaagaa gcattgaagc aagaagcaag agcagaccag
    12241 cttcgtgcag ccatcgtaga aaaagaacaa aggcaaatca gacgctattt cggtagcaaa
    12301 gagggattcg gtcaagcaat ccatgaattc ttgatgaatt atcttggcaa tgagaatcct
    12361 aataatcaat ctaatgaagg aggttccgag tatgaatgaa cacgatgaag cagaatggac
    12421 aaaaaatgaa gaacaaacag aagatcatga agaaacgaac acttcattgg aagaacaaac
    12481 aagtaatcct ctggatgaca cagaagaaat ccctatgcta gaggaaacaa aaatacacga
    12541 aaaagaaaaa gtaactccga cgaatacgaa tactgcagaa aacacccatg cttacgaaat
    12601 aactgaaaat cccgggtatt tagaaggaaa agagcaagat catcaagata tcgtcagttt
    12661 gattgaaaca gcggaagcag aattagcgga tttacgtatg agaaaagatt tgatcgaatc
    12721 agattttcca caattattag aatcatatga tcgttttata ttgaatgaag aaccaatctc
    12781 cacgatcgga aagacaagcc tgatcgaata cttcacgtat gaattactga aaccgttgaa
    12841 tgatattggt ctggaacggt caaattcagc ttatgacaca tggaaaacaa gacatttttc
    12901 gatcaattat acgttgaatg gacaaaacga attagtcttt tcctttgtat taccgaccat
    12961 cgatcaacga taccaagtgg aatcgatgag tctcctaaac gtatcaccag agacaatgga
    13021 aatcaatgtc caagatgacc gggtgctttc tttgatccga cactggtcag tggatcgagt
    13081 attctctgca gggcagatca cgatattcaa tcacaaattg aatcaattat tagcccatgc
    13141 aagaacactt ggttttaccg ttaatcagac attactagac aatacgaagc cacttcgttt
    13201 gaatcttcag agcgaatttg aattgacgaa tgaagtattg gatgatattt ttatcgtggc
    13261 aatgaaaaat ccatcctacg atatggaaaa gatcaacgaa actacttatc aagtacgatt
    13321 agataaagga cagtctttaa cgataacaac aaacgaaaaa gatcaaacag tcttgtcgat
    13381 ctcatctgga gactacccac gatctgtcat tgatttcttt atcaattacg aattcttggt
    13441 cccattaatg gtgagaaagt cctgattgga taagttggat taccagatgt cattcagata
    13501 attctgttcc aaaggacttg cggcagttat ccagaataat tgtcacaagt cttttctgta
    13561 tgtactaaat ggacgtgaat aacttgctga tgaaggaggg atttcattag gaatgtccac
    13621 aaataaaata gcacagctta gcttattgtc tgctgcttgt gtagcaggta ggatcgcttt
    13681 tcaatttatt ccgaatttcc agccaatgac tgctattttt ttatttatag tactttactt
    13741 aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt gcgatcagca gtttttattt
    13801 tggtgttgga ccatgggtca tcggccaatg gatcagctat actgttgttc tgagtttgtt
    13861 ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta tggttaaagg gagtctgttt
    13921 ttttctggcg ggcatggttt acggaatagg tatgaccgtt tttgatacgc tgttatatcg
    13981 tttgcctcaa ccttggatct attaccttca aggggtgtcg tttgatttga tgcacagtat
    14041 aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc aaacgttttt acaatcattc
    14101 aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc agtatactca
    14161 ttttgacaag ttgcagtaca acctatacac aatcggcttc caaccaaaag gaggaagtaa
    14221 aaacatcgca aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt tcggaaaaag
    14281 aagtgttctt caaaaaaggc gatgatttac tgagtatttt gaaacgaaat tttgaagtca
    14341 aagaagataa cggcttcatc acttcgcttg aagggcactc acaggatgag aaaaatcaac
    14401 tctattggat gtttactgtg gacgggaaaa gtgctactac tggtgccaaa gagatcaaat
    14461 tgaatgatgg acaatcggtc attttcaatt tgtctaaact ataagtttca taaaaaactg
    14521 ttaaagggcg aatattgcca taattcagtg agttcagttg atgaaagaag gaaaataaca
    14581 tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg gctaatattc
    14641 tagttgctat cagcaaattt gtcggctatg caatcagtgg cagtgccgcc atgctaaacg
    14701 aaagcatcca cagtatcgtt gattgcagca atcagatttt gctgttattc ggtgataaac
    14761 aagccaataa aggacagagc gagttgcatc aatttggtga aggacgagca aaatactttt
    14821 tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg gtgatggaag
    14881 cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa tccgatcatt gttatagcaa
    14941 tactgttatt tggtttggta atcgaaggaa gttcgttacg cgtggcaatg aaagaaatca
    15001 aagaattgaa tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt cgacacagtg
    15061 aaatactgat tatttttacg gaagacttgt gtgctgtaat cggtttggtc ttggctttag
    15121 caggaacaat attgacttat gcaaccggga ttgctgcatt tgatgcgatc agcggattgc
    15181 tgatcggttt cttgctgatg ggagccgcca ttttcctagc aaaagaattt tatagtctga
    15241 tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat caagttagct tttgaacgtc
    15301 ctgagatcag tcggctgatc gatataaaaa cagttcattt aagtccaacg gagattttag
    15361 ttgcagcaaa agcagatatc gttggtaaca aagagaatga agcttactcg gtcatcaatg
    15421 atattgaaaa aaatatgcgt cagtctcttc ctgacaagaa gatgtatata tacattgaaa
    15481 cagataaata tgacccaaat tattctcgta aataaaacgt ttattcatga attgaagcaa
    15541 aaatgagaaa aatataatct tgtttttaaa taaaacggct acaatttagc gaatttatgt
    15601 tacaatgagt ccgctgttta tttgttgtaa ttaaaggaga tattttaatg aacaaaaaga
    15661 aaagtgcgcc atggcttcaa gctttgcatg cagtgatgcc actttgtata agctatatac
    15721 cagtaggatt agcttgcgga gtattgttgc aaaaagttgg atttgatcct ctcttatcag
    15781 gactgttatc gattctggtc ttttccggtg gcgcgcagtt tttagttgca tctatgctta
    15841 cgacgcaagc atcatttgca acgacattat tgatggtttt ctttttagaa ttacgctata
    15901 cattattagg agcaagtctt gccggtttta tgaaaaaaga aaagcggacg tttttggctg
    15961 tcttttctca atcactgaat gatgaaaact atgcggtgaa ctacttaaag ttttcaactg
    16021 acccttcctg gaataaaaga aaggctttat atgtcaattg gttctcaatg actgcatggg
    16081 ccggtagcaa tatgatcggt aattttttcg gctcggtgat tcgagtagat gctgaccttg
    16141 tccattttgc tttgacggcc atgttcattt tcatgtttat catgcagatg aaaagcgcac
    16201 tactgatctt tacaggttta ttctcaggcg tattaggggt cgtattcatg gtattattcc
    16261 aaaatacttt cggattgatt gcagcaacag tcattgcttc atttgccgga tttatgctgg
    16321 agaaggcatt caaaaaagaa aaagctaaga aactaagaga ccgcggtaaa gatgtcaatg
    16381 agccgctctt caacttcccg gaacaggagg ccaatcacca tgactaaact atatttgcta
    16441 cttttagttg cttgtctgtt catcgtggca tatatcccgc gcttgttccc gatgctttat
    16501 tttactcatc gaaaagtgcc atcctggttc agtgactgga tgaaatatgt accggtggca
    16561 ttatttgcgg cgttagcttt taaagatgtt ttcattactc atgaacatct ggatattgct
    16621 tggaatatca aaatagctgc aatgattcta gtagcaggcg tggcttacaa aacacgttcg
    16681 atggcgttgt cagttttgac aggacttgcg tctgtctttt tgctgtctat gctttaaact
    16741 gttttatttt attaaaaaca gagattgcga cataagttgt tcaaactgat ctatccgaac
    16801 tgcatttgca acagtcaatt ctggatgcaa gtttattcgt agggataaaa aagattagtg
    16861 ttggcttatg atgcaagctc ttttttattt taggaattag agagggattt gtccttctat
    16921 gattttttgc tataataggg aagatgttta ttttatgaaa gaatagttga gggaatttaa
    16981 atgaataaaa aattgatgca aggaacattt tggctaacct ttgctaatct tctatgtaaa
    17041 gttttagggg tagtctattt gattccctgg ctaagtatga tggggaacaa tcaagatggt
    17101 atgcttgcta cgacattgta taatgttggc tatcttcctt atggattgtt cctgatgctt
    17161 ggtacagtcg gatttccaaa tgcgattgca aaaaaagtag ctgtagccac aaaagaagga
    17221 gataacgctg cttgtcggct gatttttaga agtacgatca atatcatgtt tgtcatcggt
    17281 atcgcctctg ctgcactgat gtatttattt gctcctttac tagccggaat cagtccgatt
    17341 gctaacgtta ataatgggat actagctatc agaagtttat gcccatcttt gattgctatc
    17401 ccaattctta gtgcaatgag aggatatttc caaggaaaaa atgatctgcg gccttatgga
    17461 acttctttga ttatcgaaca ggctgtgcga gtgatcgtta tcttggctgg tacttattac
    17521 ttgcgcgtat tgactgacgg aaccatatta gaagctgtat tgatcagtac agtcgcttct
    17581 ttcttcggtg ggttagctgc gattatccat atgtatatcg tcggacgcag aaaagacttc
    17641 ttcgaactta aagatttttt gatttccagc cgttatttcg aaagagaaaa ccgatctgcc
    17701 tctgtctcta tcattcggga aacactaccg tttatttttg tcggctcagt cattactatc
    17761 gttcaaatga tcgaccaagt aacgatgaaa ccactgcttc actttttccg tccagagatc
    17821 gcagatcagc aactggaatt cttattcagc cgtgcttctg tcaatccaaa taaattgaca
    17881 ttgatcctga tctccatggt aggaacagta gctataagca gcttgccaat attaagcacg
    17941 atgaagaaaa aagatcggat acaaatcgaa aaaacagtag gagatagttt ttcgattgcg
    18001 ttattgattc ttcttccttc attgactgga atgtctttat tagctggacc actatacaca
    18061 ttgttttttg gctatgatcc tgaaagtgtc ggatatttcc aaatggcttt gctagcttct
    18121 ttgtttttct ctttattcac gatcctatct acgatgcttc aatctttgaa tcaccatcgt
    18181 tttgcgatcc gattaactgt agaagcaatt atcctgaaag tcatcttcca agtaatcgga
    18241 ttaggattag ttggcggata cgggatgagc ttatcaaatg gcttagcatt cggtattgtc
    18301 tttgttcgcg gctatcactt tatgtgcaaa gaataccgaa tagcaccttt agcaaagatc
    18361 agcaattttt tcttaaaaac tttccgctcc accctgatta tgcttgcagt atgttttgcc
    18421 gttttctttt tgctgaatca gcgattgtca atggaatcaa aaagctatgc aatgatttat
    18481 tgtgtggctg taggaagtat tggcggctta gtatttttat tttctcaatt cggaaaaaat
    18541 gggtttcgaa tgttgaaaaa tgttaaacat catagcagaa gcaaagaata ggttaactaa
    18601 tcgttttttg ctgtcacttg aaaaacggca actcgtatga ttacgcttgt cgtttttcta
    18661 ttttccaatt ttagtttcat atctaatctt acttatgata tagttataag gtctggaaaa
    18721 attctttgaa acgaggaaat taaacacatg aaacttgcag tagttacaga tagttcggca
    18781 tatctgccgg aacgtatcaa gaaccatccc gacttattcg ttattccaat ccctttgatt
    18841 atggatggga aaatttataa cgaaggaatc gatatcgaag cggatgaata ttatggactt
    18901 ttaaaaaaca gtaaagattt tcctactact tcacaacctg tcgtagggga agttttgggt
    18961 ttatatgagg aattgaaaga gaaagggtat gacacgattt taagtatcca tctttcttca
    19021 gggatttctg gctttgtcaa cacattattt gcgatgaaag atgacatcag cggagtaacg
    19081 gtcattcctt atgactctaa gatcactagt atgccgatgg gacacatggt agaagctgcc
    19141 ttagacttaa atgatgaagg tcagtccctt gatgaaatac ttgcacatat cgatcgaatc
    19201 cgagataata cctacgctta tctgattgta gatgatttga acaatttagt acgaggagga
    19261 cgtttgacaa atggtgcagc gctgattggc agcttattaa aaatcaaacc gatcctgaca
    19321 ttttctgaag ggaaaattgt tttattcgaa aaaatccgtt caagtaaaaa agcatttgct
    19381 cgagctgaag aaattattgg tagaagaaat aacgaaatac aaccgccagt caaactttac
    19441 gtgatacatg caaataattt agaagtagcg attgcggaga aagagaaatt gcagaaaaaa
    19501 tatccaaatg cagaaatcga aattggtcat ttcggaccag tcattggtac acatttaggt
    19561 gaaaaagcca tcggcattgc catttcagcg caataattcc ttttattcaa aaaacatacc
    19621 aaaaatattc caacatcatc ttggactttc tacatgttac taatgagcgt gtaaaagaac
    19681 gagatggtgt ttttatttct atcaatgaag ctctttttct ttgcaatcct cacttcaatc
    19741 ctctataatg tcgcatatag tttagtaaat gagtaaacta ctaatttact tatttactaa
    19801 aaataaaagg atgaggagtt aaaataatgg aaataccaac aaatttaaaa cataaaccag
    19861 taatcgttgt agaagactat gataaagttg atggaagaaa tgcattaaat acggatgcaa
    19921 aagggctttc actgggcctt gctcagtgga atgatcgtgg aaaagtcgat atctcaggca
    19981 aggtatggcg acatactgga gaaaaatggt cacgacagtc ggaagaatta ccaatcacac
    20041 gtattttaga tttagcgatc ttagctgcac aaggaagttt atacttccaa gatgcttatc
    20101 gttatgaaaa attttatgat ccagaaaatc cagtcgtaga catcattgga ttacaaggga
    20161 accgaatgac ggtagaagta gataccaaaa atccaaagat cgatgaagac atcattttgt
    20221 attacgatac actacaaaaa gacggagaac tgattgggga acgattccgt attttaaaac
    20281 gtcttttaga tgagctagga tattaataga cagagatgat caaaagggga agagaagcat
    20341 ggagaatttc tcactaacag aaaaccgaac cttttcacgg ctagaaaagc aaatgatctg
    20401 gaagaaaccg aaaagccatc ggacaagtgc agaagaatgg cggatcgcct ccgaaatcaa
    20461 agggaattgg caagatcctg aaatgaaaat cagtaacgta cttttggaag gagatgccgg
    20521 gtcggggaag acacaattag ccaaagcact atctgctgat ctgcaacttc cctatacaaa
    20581 agtgacctgt tttgcagata tggacaaatc agatgttttc ggagccttat tacctgtagt
    20641 agattctaat aaggaacaag accaagaatt actagaagct atttaccaaa cagatacact
    20701 tgaagctgta ctgacactga tccagaacca ttacggcatt gatccttttt ctgctaaaga
    20761 aaaattagca gagctagttc aaaggatcga agctgattat ccagatgcaa tccattacag
    20821 ctattaccct tctgaaatcg ttcgagcgat tgaaaaagga tatttattag agattcaaga
    20881 gccgactgtc atccgagatg cctctgttct tgtggcactt aactcagcgc tcgaaccaaa
    20941 tgggatgttg aatttaccga caggaatcat tcgtcgccat ccagattgcg tcattgtgat
    21001 cactacaaat cgaaattacc aaggaaatcg cccattaaat gaatctttaa gagatcggat
    21061 gcaacatgcg gaaaaaatgg atcttcctgc cttagaagtc atggcagaac gtgcgatggc
    21121 gaaaactatg atccaaaaac aagaacttct cttaaagatg gcagagatta tccgtttgtt
    21181 ggatgaaact gcaaaagcta acgccatcaa aggtgtagca ggaatgcgtt cttattttta
    21241 ttgggcaaat acctataagc aaggacaaga tttactccag tcgatttatc caaaagtttt
    21301 gtataaatta tccactgatt cagatgaatt ggcaattctt gaacaagctt tgtctgacag
    21361 tggattgcta ctggaacttc aggacatatt gagaaaagaa aaatggggtc atcctcaatc
    21421 tgagcataca aaaggaagaa ccattagcgc cgaagaagca aatgaacgaa acatcactga
    21481 aaatgctgaa gaaattctgc gcttagatga aaagaaatcg ttgtctgctg aagaaccaaa
    21541 tgagggagaa gagaaacaag aagaaacaat agaaaaaaac gagattcctc ctcaaatgca
    21601 aaaagaacgc agtagcgata tcgaaggaaa aaatcaaaaa gaacaacaaa caggagatga
    21661 ttctcaagaa atgtctgctt ctgacatgga agcacatgat aaggaaatca agaagcaact
    21721 gaataaagaa gcgcgacaaa tcatgaagga aacgattcat gaaaaagaag gtctcatcgt
    21781 tcaccgacca gctttccaag gaaaaacgcc ggaagcacgt gagctacaca tgcaagtaat
    21841 gccaatagta gaatcactta gccggcaaat tttagagtta ctggataatg aacaaacaga
    21901 gacgtaccaa aaaggaaaat acgaaggtca gcggttcaat gcaagcaaag tagcatatgg
    21961 tgatttgaga aattttgaca aaaagaaccc gccacatgag caaccttctt tagcggtagc
    22021 tgtacggatc gatgaatctg ggtcaatgat acgggatgat cggatcgaag cggcaaaaaa
    22081 agcagcgatt gccattgccg aattttctaa gaaagtcgat atcccattgt tgatttacgg
    22141 agatacagca gaccgttcgt caagagaaaa gacttctttg ttttcttata aagaatttga
    22201 agacggattt caatggttaa atgaaaaatt agtcactatg aaaccaaggc aaaacaatcg
    22261 tgatggtgct gcacttcgtt tagcagcaga aaaattaaac agacaatccg caacaaccaa
    22321 actgttgctt aatattagtg acggtcagcc aaaagcgttg ccggattata caggcaaaaa
    22381 agcaaaagaa gatatccagt ctgttttgaa agaatatgaa cgtcaaggaa tcctatttgt
    22441 ctccgcagct attggccaag acaaagaaga aatcaagtcg atctacggtg aaagtcgttt
    22501 tgtagatatt actgacttga atgaatttcc caagcaaatg attcaactga ttgcccgcta
    22561 tctatgataa aataaagctc aacgaacgca cttagcagat tcgttgagct tttcgtttct
    22621 agttaccggt aacaaggata cttctaaaat agaatcggtt ataatcaaga gggaccgtta
    22681 aaggagtgaa acgatatgat acaagaacaa aaatggtgga aagaagcagt aggctatcaa
    22741 atctatcctg ctagtttcaa agatagcaat ggcgatggtc aaggagacat caatgggatc
    22801 cgtgaaaaac ttaattattt gaaagatctt ggtatcggct ttatctggat caatccgatc
    22861 tatcaatctc catttgtcga taatggttat gacgtaagtg actatcaaga tatagaagag
    22921 agattcggaa cgatggaaga ttttgattta ttgctaaaag aagcacatga attagggatc
    22981 aaagtgatta tggatcttgt catcaaccat agttcggatc agcatcaatg gtttgaagaa
    23041 tcgaaaaaat ctaaagaaag cccgtatcgc gattattaca tttgggtcga cggggtagat
    23101 ggcaaagaac cgaataattg gacatcgatt ttcggcggtt ctgcttggga gtattctcat
    23161 gaaactggac agtattatct gcatgttttt gcaaaagaac aaccagactt aaattgggaa
    23221 agcgagaaat tgaaagaaga actattcaat atgatccgat ggtggttgga taaagggata
    23281 gatggatttc gtttagatgc gatttctcat gtaaaaaaag atgaatattc agtaaaagcc
    23341 acagagaacc ctttctcacc ctttcaaaat gtctcaggaa tcgaagaaca tttgaccgat
    23401 ttgaaacatg tttttgaaga gtacgatatc atgactgtcg gagaagcaag tggtgtcact
    23461 gcagaagaag gaccacaatg ggtagggaaa gacggttatt tcgatatgat attcgaattt
    23521 gatcatatcc atatctggca gcaagaaaaa gaagggcaat tagatgtcct taaattgaaa
    23581 cttgcattaa gcgcttggca aacttcattg gatggtatcg gatggaacgc tttatatatg
    23641 gaaaaccatg atgttcctcg tgccgtttca gtgtttggag atactcgtcc cgatttttgg
    23701 gctatgtctg caaaagcaat cgctatgatg tatttctttt tgcaaggtac gccatttatt
    23761 tatcaaggac aggaaatcgg aatgacgaat atgccctttg aatcgatcga tcaagttgat
    23821 gcagtagatt cgaaaagact ttataaaagg ctgcttgcgg aaggtaagac aagagaagaa
    23881 gcattggata tcattcgtga aacaacgaga gacaacagca ggacacccat gcaatggact
    23941 tcggaacaat atgctggttt ttctacacat gaaccttggc tgatcactaa tccaaatacc
    24001 aaaacgatca atgtagaaca acaagaatat gaaccagagt cggtcctgca attctataag
    24061 aacatgatcc gcattagaca aaccaataaa ggtctgatat acggttctta taaagaatat
    24121 cttcatgaac atccacaact ttatgtctat gaaagatatt tagaagatga acaatatctt
    24181 attatggtaa acttaacaga atcacttgct gactatgaac ttccaaaaga agcagatcag
    24241 tcctggacat tactcttgtc aaattcctct agcggagaat ttgaagctaa aggcatacta
    24301 gcaccttatg aagctcggtt atataaaaca aacaagtaaa acttcctcgt taaagttcat
    24361 tagttggact ttgcgaggaa gtttttcaga ttgctctgtc aattactcat ttcaataatt
    24421 cgctctagga ccaccgatat atttaggacg agagcgaagg gctgtcacgt gagcttgtcc
    24481 aagggttttc aaacgaggtc tgacaggtac ttggacatgt ttgatatgca taccgattga
    24541 tgtgtcaccg atatcgatac cggcttgtcc aactacgtgt tccacttcga caggatcatt
    24601 gaactggtca aaagcagcaa cagaacaagc acctccagca tgtagtgcag gaacaacaga
    24661 gacaatttcg aaattttttg cttctgccac tgttcgttct actactaatg ctcggttcaa
    24721 atgttcacaa ccttggacag ctagcgcaat ttctttagga tccaacaact cttttaacgt
    24781 acggatgatc cattgaccaa cttccgcact ggaatttttt ccaatatgtc ctccaacgac
    24841 ttcgcttgtg ctgcatccta aaacaaaaag atcacctttt ttcaagtgtg cctcagccaa
    24901 tatgtcatta actatttcag tcaattggtc ttttaattct ttttctgaca acttcatttt
    24961 ctaaaacctt ctttttcaat taatattgct tccgtgcttg aatacgttga agacttgcag
    25021 ttataggaaa ggcaagtccc attccaatca gattttggaa gatgtttcct ggtatagaag
    25081 ccactccagc cggccaatca tataaaatcc aaccggcgat accatagccg atgatcataa
    25141 ctaagcttcc taataaaagc cctaatcccc acatacgagt agaagatttg ctgaaagaac
    25201 cagcaacata tccttgtaac ccgtggatca ctaatgaaaa aactgcccac tgcggatatc
    25261 ctgaaatcaa atcgatcaat agaccactgg aagcaccaac aactaatcca cccgttggac
    25321 cataaagaaa agcagcggta tagatcccgg cctcgcataa tgtcacaaat ccattcgtag
    25381 ccggtacagg gatcaaaatt gttaatgaaa gggcaactgt caacgcaatc agcatagcat
    25441 agatcgttaa ttgcttagtt ttcatttctc attcctcctt tttgaatacc gccagaccag
    25501 acacttccgg tatgatcgtt catcagcacg ccattagaaa tacaatgggc aacaaatttt
    25561 ttactttctg ctactgcttt ggtaaaatct gcccctaaca caagattcgc tgttattgca
    25621 gaagaaaagg cacaaccggc cccgtttact gttgtagtgt cgattttttt atgagaaaat
    25681 acctcataat gatcaattga atagagaaga tccacagctt catctccagc catccttgtg
    25741 ccgcctttga tcacaatgtt ttttgttcct gaagcagtga gttctttagc gagatggatc
    25801 atttctgtta acgaagaggg aagagcttga ccagaaagta aagcagcttc tgccaaatta
    25861 ggcgtaatca tcgtaacaag tggaaaaagt tcttcgatga gctgcgaaat atacaattga
    25921 ttgatcacag cttcagtttc tttgaatgcc aaaacagggt ctaaaatgat tgggatattt
    25981 tgttttgttt ttatgaagtc tcgaacaacc atgattgctt cacttgaatt caataaccca
    26041 attttgatac catctaatga agtcatttta tcaatcgaaa ctaactgtcg tttaagcaat
    26101 tccaaagata tagattccag cataaatcgc tcattaatat ctagtgcccc tatagaagtc
    26161 aaggcgctta gtccgaatag accaaaattc tcaaaagttt ttaaatctgt ttggattcct
    26221 ccaccagcaa atggatcaga accgccaatt gtcaagattc ttttcataac attttcctcc
    26281 atgaataaga gtataacgaa attttcaaaa aagaaaaaca gccaattggc atatttttat
    26341 ctcatccaat ttaaaaaaac aaaaagaaaa gaaagaccaa aatggatctt tctttcctta
    26401 aatagttagg acttttgtga ttttaattct ttctcgattt gaagccgttg cactttcatt
    26461 gtagctgttc gcgggattgc atcaaaatca cggatgatag ggacgttcaa gcgaggaagt
    26521 tcatggactt gctcccacca tcgattccaa tccatttctt tatcaggagc taaagcaata
    26581 atcggttgtg gagaatgatt tttatccctt acaatcacta cttctgccaa aaaatctaac
    26641 gaatctaata aaaagtcttc aatagctaat gtactattga tcgtttcgat caaatccact
    26701 tgccgatctt tcaaaaatag gtggccatgc ttatccataa aaccgtaatc tccgctatcc
    26761 caccaatcgc cataaacatt ttcctggaac cgtgcatctt ccttgtaata agtcaaagcc
    26821 cgtcctttag acagtaattg tatatgacca tctgtgtttt caggtaggac attcccaact
    26881 gaatctgtaa tccgagcgct tgtcatgtct tccagtccaa cgcccatatc tcttgcatca
    26941 cttgttttca aactctctag tgtatgagcc ttaagaatca ttggtccgca ttcactttgg
    27001 ccatatactt gaagaaatac cgcatcctgt gccatcgacg ttcgcaaaaa tgtcttcatt
    27061 gtctggttgt ttatagcatc aaaagtggag tgatagtatc ggatacccga aaaagcttct
    27121 ggatgctcct tagcagtaaa acgccactga acaaagttat tcggatgggt ctctagtgca
    27181 attggccggt gagcttccaa catatgaaca acttttgaac tttgtgcgtt tgctaaaggc
    27241 atcataggaa atcccatgga catcaaagaa gagataccaa tattgaatct ggaatgcact
    27301 ggagagatgt gaaaagcgat taatttcttt tccgaaattt ttgtgaaaac tgttttttgc
    27361 catttcgtcc gccatcccat agaatgagca gaatgacaaa taagtttagg gataccagtt
    27421 gtaccagaag tatgggtcat ataagaaatt tcatttttgt ctaaaaatga ttgagaaaca
    27481 gacaaagcag gttgttccat taaataaaga ataggaa
//
LOCUS       DACDTH010000033        26269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDTH010000033 DACDTH010000000
VERSION     DACDTH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.033"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            11..733
                     /locus_tag="IWK54_001544"
     CDS             11..733
                     /locus_tag="IWK54_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9593669.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            791..2830
                     /locus_tag="IWK54_001545"
     CDS             791..2830
                     /locus_tag="IWK54_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9593670.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2939..4090)
                     /locus_tag="IWK54_001546"
     CDS             complement(2939..4090)
                     /locus_tag="IWK54_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9593671.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4244..4774)
                     /locus_tag="IWK54_001547"
     CDS             complement(4244..4774)
                     /locus_tag="IWK54_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593672.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4873..5223)
                     /locus_tag="IWK54_001548"
     CDS             complement(4873..5223)
                     /locus_tag="IWK54_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9593673.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="IWK54_001549"
     CDS             complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="IWK54_001549"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9593674.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="IWK54_001550"
     CDS             complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="IWK54_001550"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9593675.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7626..8288)
                     /locus_tag="IWK54_001551"
     CDS             complement(7626..8288)
                     /locus_tag="IWK54_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9593676.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8472..10118
                     /locus_tag="IWK54_001552"
     CDS             8472..10118
                     /locus_tag="IWK54_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9593677.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10109..10237
                     /locus_tag="IWK54_001553"
     CDS             10109..10237
                     /locus_tag="IWK54_001553"
                     /inference="COORDINATES: protein motif:HMM:NF015727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593678.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDHRKKRLP"
     gene            10204..11268
                     /gene="uxuA"
                     /locus_tag="IWK54_001554"
     CDS             10204..11268
                     /gene="uxuA"
                     /locus_tag="IWK54_001554"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9593679.1"
                     /translation="MDGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPRERIKEL
                     KEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVICYNFMP
                     VFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEPERLAHI
                     KSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLPRIVKNR
                     EDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRNIKFLAN
                     RDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGLYDRALG
                     AVYLNGLWEAIEKGNKSKDN"
     gene            complement(11376..12929)
                     /locus_tag="IWK54_001555"
     CDS             complement(11376..12929)
                     /locus_tag="IWK54_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9593680.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            13077..14365
                     /locus_tag="IWK54_001556"
                     /pseudo
     CDS             13077..14365
                     /locus_tag="IWK54_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14491..15570)
                     /locus_tag="IWK54_001557"
     CDS             complement(14491..15570)
                     /locus_tag="IWK54_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9593681.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15673..16113)
                     /locus_tag="IWK54_001558"
     CDS             complement(15673..16113)
                     /locus_tag="IWK54_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9593682.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16170..16580)
                     /locus_tag="IWK54_001559"
     CDS             complement(16170..16580)
                     /locus_tag="IWK54_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9593683.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16760..17194)
                     /locus_tag="IWK54_001560"
     CDS             complement(16760..17194)
                     /locus_tag="IWK54_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9593684.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17294..18142)
                     /locus_tag="IWK54_001561"
     CDS             complement(17294..18142)
                     /locus_tag="IWK54_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9593685.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18531..20297
                     /locus_tag="IWK54_001562"
     CDS             18531..20297
                     /locus_tag="IWK54_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9593686.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20399..21658
                     /locus_tag="IWK54_001563"
     CDS             20399..21658
                     /locus_tag="IWK54_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9593687.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21805..23106
                     /locus_tag="IWK54_001564"
     CDS             21805..23106
                     /locus_tag="IWK54_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9593688.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            23109..23960
                     /locus_tag="IWK54_001565"
     CDS             23109..23960
                     /locus_tag="IWK54_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9593689.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23973..24788
                     /locus_tag="IWK54_001566"
     CDS             23973..24788
                     /locus_tag="IWK54_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9593690.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24939..26105
                     /locus_tag="IWK54_001567"
     CDS             24939..26105
                     /locus_tag="IWK54_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9593691.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         8901 a         4770 c         4880 g         7718 t
ORIGIN      
        1 attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg agtttatcca
       61 tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga aaagtatccc
      121 taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg aaaacggtcg
      181 ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc
      241 tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc
      301 ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga
      361 agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt
      421 tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa
      481 caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga
      541 caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca
      601 gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg
      661 tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa
      721 agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
      781 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
      841 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
      901 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
      961 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
     1021 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
     1081 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
     1141 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
     1201 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
     1261 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
     1321 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
     1381 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
     1441 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
     1501 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
     1561 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
     1621 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
     1681 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     1741 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     1801 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga
     1861 cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     1921 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     1981 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     2041 ttatgctcct ggatctacgt ttaaaataat tacagcagca atcggtctcg acaacggcac
     2101 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     2161 gggatctttt caaacaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc
     2221 tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa
     2281 atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     2341 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     2401 tggatatgga cagggcgaac ttctaattaa tcctatccag caagcagcaa tgtattctgt
     2461 ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     2521 taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt
     2581 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     2641 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt
     2701 cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     2761 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
     2821 ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt
     2881 ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc
     2941 acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta
     3001 ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc
     3061 caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca
     3121 atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg
     3181 tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa
     3241 aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag
     3301 cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa
     3361 aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac
     3421 aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa
     3481 gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt
     3541 caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc
     3601 tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg
     3661 atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag
     3721 aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt
     3781 tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac
     3841 caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta
     3901 ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca
     3961 atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta
     4021 atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa
     4081 taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca
     4141 ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg
     4201 gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt atagtcattt
     4261 ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa ataaagccga
     4321 atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg aatccgccaa
     4381 tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc atgctgaaga
     4441 acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc aggatagaaa
     4501 cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa agaccactga
     4561 acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca aaaagattca
     4621 acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga aaaacagcag
     4681 aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt aataaggcgt
     4741 atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt tgacttattt
     4801 taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag gagaagaata
     4861 tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg ctagctcatc
     4921 gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt cttctactcg
     4981 tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag gggcttccgc
     5041 aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg tttgggaata
     5101 acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt ttctctccgc
     5161 ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc
     5221 cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata taagaaaaag
     5281 agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat
     5341 ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca tcagataatc
     5401 gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt gtttgtatgg
     5461 actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc catttttctc
     5521 ggtcactatt tcatttcttg gattcatttc gatcgttcca tctagccaat ccgtattagg
     5581 gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct ccatcgtacg
     5641 gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt tcgtcgctgc
     5701 atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga tttgatctgc
     5761 ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag ccaaatcgat
     5821 tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc ctttaaataa
     5881 agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt tttcgcctgg
     5941 tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga
     6001 tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg aaacggcctg
     6061 ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt gctgcatcaa
     6121 ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg tttcaacgac
     6181 ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg ctgcatagat
     6241 agctgcacta ctagctgctg gtccaccgcc aattactaat acatcataag gttctttttc
     6301 aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt cgatcgtcat
     6361 gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca tgattttttt
     6421 atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa tatttggatt
     6481 taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac aagtcaagct
     6541 aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac tcaaagattc
     6601 actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag aggaaaattc
     6661 atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg gattgataga
     6721 aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag acatttctgc
     6781 tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca aactaacaga
     6841 aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt tagtttcttt
     6901 gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt tcaatgtttc
     6961 gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta catattgagc
     7021 agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg cattgatttc
     7081 catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag ctaatccttg
     7141 ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac cgatcatcac
     7201 gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag taaagtgggt
     7261 atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt attgttcttg
     7321 taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga agcaaagaat
     7381 actccagttc cccataagat ctttttcact gacttctaaa aattctcctt gatgataagc
     7441 atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac ctccaataat
     7501 aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg gatattttgt
     7561 tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga
     7621 aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg ttctttcagc
     7681 atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt atcaactaaa
     7741 gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc ctgaatggtc
     7801 actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca gttccaaact
     7861 tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc acggaaaaaa
     7921 gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt ttctagcact
     7981 attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc ttccaactgt
     8041 tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac atacgtacca
     8101 ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg
     8161 ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt ccttccaggt
     8221 tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga ttgcaagctg
     8281 cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag aagaggtctg
     8341 aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa aattttggtt
     8401 gacatagcga tttttattgt ataaaattca cttgaataca aaaactaata tgttagttta
     8461 ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa ttatctcaaa
     8521 aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga caaaaaaatc
     8581 ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca gaaatcgctc
     8641 aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg
     8701 aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa gtggtcatgc
     8761 caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt ctgtattgtc
     8821 atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat cctagtttac
     8881 aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca aataataaat
     8941 atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca catacaatga
     9001 gtattcttgc tgcgctactt tataaacgtt ttttatctgg agcttggccg attgcacttg
     9061 tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg ctaacgatcg
     9121 caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac ttatcgaatg
     9181 aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat ccttccttgt
     9241 cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc caaaccgcaa
     9301 aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta atcatggaag
     9361 atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg actgatcgtg
     9421 caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct ttgcatacgt
     9481 ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag atgaaagatc
     9541 cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca gttgtggagt
     9601 ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg cgcttttcta
     9661 atccaaatat tcctgatatg cctcagcgaa tcgccacgga tacctctcaa aaggtggcga
     9721 ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac ccgcagacac
     9781 ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc attgatgatc
     9841 aaggaaaacc attcatcccg agtccagacc cactattgac agactttcag cataagcttt
     9901 ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg gctcctattt
     9961 tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc agaaaaatag
    10021 aagaaatgtt catcaagatg gtagcaggac cgaatgccgt acgaaaaaca ttacaggatt
    10081 acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt acggatcaca
    10141 ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag ggattgtagg
    10201 cacattgatg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
    10261 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcccagggag
    10321 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
    10381 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
    10441 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
    10501 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
    10561 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
    10621 atcgaacatt ccagcaatga atttgaattg ccgggatggg aacctgagcg cttagctcat
    10681 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
    10741 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
    10801 catccagatg atccgccata ctcgattttt ggattacctc gaatcgtaaa gaatcgagaa
    10861 gatttagatt ggttgtgccg catagtagac agcccttata acggaatcac tctttgtaca
    10921 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgtcaa
    10981 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
    11041 tatgaggcac cgcataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
    11101 atgtatgaca acggatttaa cgaatacatc cgtcctgatc atggaagaat gatctggaat
    11161 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
    11221 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
    11281 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
    11341 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
    11401 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
    11461 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
    11521 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
    11581 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
    11641 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
    11701 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
    11761 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
    11821 gtttggactt ctttcaacac gctatgttct tgataaagca tgaaagtagg aaggggcgta
    11881 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
    11941 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaaa ggactccacc
    12001 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
    12061 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
    12121 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gggatcaata
    12181 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
    12241 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
    12301 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
    12361 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
    12421 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
    12481 atagacaaac cgtacaatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
    12541 gaggcactat agtttgtttg ctgacttaaa ctgataatcg aatttgtctg acagctgaac
    12601 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
    12661 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
    12721 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
    12781 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
    12841 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagaga
    12901 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
    12961 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
    13021 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
    13081 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
    13141 agacagtcca cacagaggag atagaaacag ccaaatggac agaaaccaag tgggccaacg
    13201 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
    13261 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcaggagac ttagcaccta
    13321 tactacaatc agctgttaca tttgagaatg ttgatcccgc tttgtcgatt ggctatgacg
    13381 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
    13441 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
    13501 atcatcattt aacgccgtat agtcagcggc agacaattga gcaagattca gctatcgact
    13561 caggaggaga tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat aagccagaag
    13621 taccgcctac agaaaatcca gacggagaac agaaaccgga aatagaacct ggagaagaac
    13681 ccgatacaga aactcaacca gagccagaca atgaatcaaa acctgaaata acccctggag
    13741 aaaaaccaga tgtagaccca gaagaaaaac cagatgtgac tccagaacca gatacggatt
    13801 ctggaaacca aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag
    13861 aaaaaccaga ggtagcccca gaagaaaaac cagatgtaac tccagaacca gatacggatt
    13921 ctggaaacca aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag
    13981 aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg
    14041 caagagacca aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa
    14101 aagagagtaa aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa
    14161 aatcacgaat atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa
    14221 cctttgctgt cctgaaacga aaaataaaca gctaccaaaa tccggagagt cacaaaacaa
    14281 agttatactg tggagcggaa tcattctatt aagtatagca acaatgcttt ctgcaaaaag
    14341 atttaaacaa aacagatctc tctaacattt gaataatgtc tgtgaacgca tttttcaaat
    14401 aaaaagaaat atccagaaaa tgaccccaaa ataccagatt gaaatctgat atttggggtc
    14461 gttcatactt tatctttctt catatttatt ttaagggata tctgtttctt ttggaagatg
    14521 gttcgggatc tttttcactt tcaatacatc taaaaagaag gtaaagatcg gaatgccgac
    14581 aatcaagccc caaacgccaa agaaccgttc gctaacaagt aagaccacaa atgtataaaa
    14641 aatcggcagt tctgttttgc tggacatcaa cttcggattc agcacataag actcaattag
    14701 atggacaatc aaaatcgtaa tcaggatata aacaacatct cggatgcctc cttgggaata
    14761 agctatgaaa gatagaggaa tacaagaaat gataacacct gctactggga tcagacttaa
    14821 aatgaaaatc atgatcgcta aactcagcag ctgatgaaag ccaaatacag ccaaggcgat
    14881 tgtagtgagc aacgtattta aaagagcgac aacaaattga gcttctaata ccaaaccaaa
    14941 tgtattgaca aacttatcgg caaaatagta aatatcttga aagaaccaag aaaattcact
    15001 ttttaaaaac aatcttgaga atacaattgt ttttttcttt tcaatcataa aaaagaaact
    15061 taagatgaat gacatagcaa acgccactgc catttttcct atatcctgta aatagccaag
    15121 cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg tttcgctcaa tataagtatg
    15181 gatatattgt aaaacaggat ctgcatcttt tggctgatgc tcgtaaaaat taataacgga
    15241 ctggatcatg tcaaatgttt gatgaataag catcgggata tactttgtga atgccagata
    15301 gataaggtaa acgatcagtg tataaatcat gatagctaaa acacctgtag ggattcggac
    15361 aaatcgctga atgatttgaa caagatgaat catcaaataa gtaaagataa atgtgagcaa
    15421 aatggttgta atcatacttc taactaagaa aagaacaaaa acgacaaata ataaaacagt
    15481 gagacggcgt attttttcat tcgccaaaaa acgctgataa acggtcaaat cgacaacctc
    15541 caaataacaa aaaataaaca cgttattcat tatatgatgg aatatgaaaa taagaaagag
    15601 caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt
    15661 catctagcag tttcagccct ttttctggtc ttgatgaaaa ttatctagca tttttcgggt
    15721 cagatgttgc agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg
    15781 tggaatacct tctgcttgtt tttgcaacgc agcaccttga gcagtcagcg taacatttac
    15841 gattctttca tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt
    15901 tagtaaggga gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga
    15961 agtctttttc ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag
    16021 cggcttgagg aggggagtgt agtgtctaat gatttcttta gaggcggcat ataaaggaaa
    16081 acatacttga ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag
    16141 tgtatcatag cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct
    16201 atatttccag ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct
    16261 tgcgtttctt ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag
    16321 ctcgtttcac tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata
    16381 tttttttgct gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca
    16441 aacaattgtt cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat
    16501 tcgaatgtac gatcgggatt atgcacttct ccagagcgtc cgcctgtgtt gatcattttc
    16561 gtagaataca tttttttcat aaaattacca cctttaatta tattttatac aattgaattt
    16621 tacacaatat aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc
    16681 acatgaaaca gacgcgcttt ctcgaatagc aaacatatac aaaaaatatc agagacaaaa
    16741 aataatctca tcataaacat tacatatatt tctcaaaaag ttgttttctt tcttcaaaaa
    16801 ttacttcacg atggacctga tgaagcatcc ataaataacc aaaaggatct ctgaacattg
    16861 agttagtcac accaaagtcc atcatttctg tcatttcttg aatctctaca cagccatttc
    16921 gtaatgcttt attatgtgtg tcctcgatat cagagacgat gatattgaac cagatcgtat
    16981 ttggatgttc agcagtagga gcagtcaaac caagagacgt gttttcatcc agcatatgga
    17041 atctcgtacc atacaaggta aaaatgactt cattattgcc tctagggtaa tctgttgcct
    17101 caatcagttg tatttcatca aagattgttt gatacagttc tagtgctttc aaactatcat
    17161 ttaatacaaa atcgatttcc gttcctttat gcataaagat ctcctctcct tttcagtcga
    17221 acagttacta gattctttta ttataccagc taaagaaaac gcattcaaaa aaatagcgga
    17281 tttacttacc aactcattcg attggatgat aagtaaatcc gcgtcccatt acttctgcta
    17341 cgctgctgac aatgacaaag gcacttggat cgatttcttc tatcaatggt ttcattcggg
    17401 aaaattcatg atcagtcaca acgatcaata aaacctcttt tgactttcga ttatacccgc
    17461 cttttgcctc tagcagggta aggccacgtc caatctcagc acttaagcga aggcggatgt
    17521 cttccaaata atgttcactc atgaccataa cagatttctt tcgattgata cctgtttcga
    17581 tataagtcat gacaattgaa gtgattgcaa tagataaaat agcataaaga aactcttcta
    17641 ttccaaaaat aaacagatta agagaaacaa cgaatgcgtc tgtaaaaaat aagccaatcg
    17701 aagtattcaa gtggaaatat tttttaaaaa tgaggggagg gatcgtcgta ccgccgctag
    17761 aagagttgtt tttatataaa atcgcaactc ctaaagcaaa aatcgcactt ccaaaaatga
    17821 cagacaacaa ccgatctgaa gtaatcattg tttctggaac tattgcaatg attaaaggaa
    17881 aaaccaagct tccgaaaagt actttaaaaa agggtttttt acctaaaaat agaagagcta
    17941 gtaccaacat aacgatattc aacactaaaa taactatagc tcggtcaaag cctaaagcag
    18001 cttccagtaa tattcctaga ccgctgacac ctccagcagc aatatgatga ggccctaaaa
    18061 acatattgat agcgactcct aatatgaaga gggcaaaaat aatcttaatg tatgtccata
    18121 taaattgtct cgtttctttc atttattcac ttccttaatt cattaataat actcttatct
    18181 tattataact tacttttcac cttaagttaa ctttaattaa taaaaaaatt aggctagtct
    18241 aaattattat gtttcattct tctagaatat acttctacgc caaaaactgt taaaaaactt
    18301 ttactttaga aaattggaga aattgatcgc ttaagataaa ttcaggacac taaatagtac
    18361 aggaagtaga ctaaaacttt taaaagtttt aagtctatct atttggcaag tttaaaaaat
    18421 aaaaattcgg tatcaaaaaa ctgttaccga taacattttt gaaagcgttg tcttttgctt
    18481 ctctatcact tatagtgatg acgtcatatt aaaacacaag gaggaaatca atggatacag
    18541 ctgcaattta tcatcgaccg gaaagtgaat atgcatttct atatacgaat acaagatttc
    18601 gtattcggct tcgtactagg aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc
    18661 catataccat aacaactgaa aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa
    18721 gtaccaatgt tcatgattat tgggaaattg aagtgacgag cgaaacaagt cgcttacagt
    18781 atgcgttcca tgttgttggg gaagataaca cggactgttt ttacggtgat caaggaattt
    18841 ttccttatcg agaaaatgtg attactgaac caaattttta ttttcgaata ccttattttc
    18901 atcaaatcga tcgattcact attccagaat gggtaaaaga aacagtatgg tatcaaattt
    18961 ttcccgaaag atttgcaaac ggggacaaag caaatgaccc cgaacacact ttaccttggg
    19021 gaagtaaaga tccagaccga gaagactttt tcgggggaga tttgcaaggt gtgatggatc
    19081 atttggatta cttgacggac cttggcatca atggcatcta tttttgtccc atatttaaag
    19141 ccacatccaa tcataaatac gatacgattg attattatga ggtggatccc gcttttggcg
    19201 ataaaaaact tttaaaaaac ttgattgatg aagcacataa aagagggata cgtatcatgt
    19261 tcgatgctgt attcaatcat atgggtgtcc attcaccaca gtggcaagac gttctcaaaa
    19321 acggagaaaa atcgatctat aaagactggt tccacatccg ttttttccca gtagattctt
    19381 atcagatgac tgacttacca gaaactgctg aaaatatccc atatgatact tttgctttta
    19441 ctccattcat gccgaaacta aatacagcaa atccagaagt gcaaaaatat ttgctggata
    19501 tcgcaactta ttggataaaa gaattcgata ttgatggatg gcgtcttgat gttgctaatg
    19561 aagtcgatca tcatttttgg aaaaaattca gagaagcagt tacagaaatc aaaccagaca
    19621 tctatatcct tggagagatt tggcattcct ctcaagcttg gctgcaaggg gatgaattcc
    19681 atgcagtgat gaattatgca tttacagatt cgattaaaga ttattttgca aaaaagaaaa
    19741 tcacagcttc acaaatggtc agtggaatga atcatcagca aatgctttac agagatcagg
    19801 tgaacgaagg aacattcaac ttattagatt ctcatgatac tgccaggatc ttgacccttt
    19861 gccaaggtaa caaagaattg atgaagtctg tgatggcctt tatgtttctt caaaaagggt
    19921 ctccttgtat ctattacggg acagaaatag ggatgactgg tgaagatgac ccggattgcc
    19981 gcaaatgtat gatatgggag aaggaggaac aagatctaga gctattcggt tttataaaag
    20041 aacttgtttc tctaaggaaa cagttgtcta agattatttc tgaaggttct actcagtggc
    20101 taatagtaaa tgatcgtgaa gataagctct atttcacacg gaaactagag ggacagatca
    20161 tctatgttta ctttaatcag tcaaaagaac cttgggtagt agaacaggaa aatgaggtga
    20221 ttcttagtca aaattgtcag ttgttagaag acgggaaagc agagatacga caatacggat
    20281 ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg caaatgaaga
    20341 tttgtgccca ttataataag ggtgtaacag cacgttgaat acaaaggagg aaatgaagat
    20401 gaaaaaaaga agtgtgaaac agttgggttt agggttaatc tctttaggaa tcttaggcgg
    20461 attagcggct tgtggcaatg gtaactcttc agcagaaggg gaagccgatg aaggaaaaac
    20521 gttaacagtt tccgttgatt ctggatataa agattatata aacgaaataa aaaacacttt
    20581 cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag gacatgttcg atcagttgga
    20641 atcattagcg ttagatgggc ctgctggaaa gggacctgac gttatgatgg cagcatatga
    20701 ccggatcggt gcgttaggac agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc
    20761 agcttacgat gaaacagaca aagcccaagt gacttatgat ggaaaaatct acggagaacc
    20821 tgcagtaatc gagacccttg ttttgtacta taacaaagat ttagtcgata cagcaccatc
    20881 aacgtttaaa gatttggaga atctatccaa agacagtcgt tttgcttttg agtcagaagc
    20941 aggaaaaaat acaggatttt tagctaaatg gactgacttt tattattcct atggtttaat
    21001 tgcaggttac ggtggctatg tatttggtga tgatggcaca aatccttcag atatcggttt
    21061 gaataatgca ggtgctgtag aaggaatctc ttatgcaaca gattggttca aaaatgtttg
    21121 gccaaaggga atgcaagaca taaaaagtgc aggagacttc gccagccagc aattcatgtc
    21181 caataaaact gcagcaatca ttgacggtcc atggcaagcg caaacataca aagaaaacaa
    21241 tatcaattat ggtgtagcca aaattcctac tctaaataat ggacagcctt atcaaccgtt
    21301 tggcggagga aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa
    21361 atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga
    21421 aatccctgca aaccaacaag ctagggaaac agcaaaggct aaaaacgatg aattaacaac
    21481 agctgtgatt gaacaatatg aaacagctca agcgatgcca aacatcccag aaatgggcga
    21541 agtttggaca ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa
    21601 agaatccgca gatgaagcag taaaaacaat cagtgaagcc atagaacaaa aatactgatt
    21661 aaaatgataa ccgtccattt ttataaaaaa tggaacaggt gcttcagata aaagtggcaa
    21721 gagcgagatg gaaaattatc ttgctcttgt tgccattatc ttaaaagcag tttgcggttt
    21781 agcaagttta gagggggcat tcagatgcag caagataaaa caagcaatat caacgaaaaa
    21841 aaaatcacgc tttgttcact tattccaggg atggggcagt ttgtaaatgg ccaagctttt
    21901 aaaggtatcg tttttttcgc catattcgtc ctattcattg gtcagatgct tctgggcgga
    21961 tttcatgcat tggtaggatt ggttactttg ggaagtatac caatggaaga tcattcatta
    22021 tttattttga ttgaaggcac tttgcaattc atcattacaa tcattttctt gattttttac
    22081 ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc
    22141 aatcggacga tgaaggaagt catacataat ataggtgatg aaggttttcc ttatctattg
    22201 acacttcccg cttatcttct catgattttt acgatcattt tcccagtcct agttacattg
    22261 ttcactgcat ttactaatta tgacttcaat catattccac cagccagttt aattgattgg
    22321 gtaggggcta aaaacttttt tagtatcttc tttttaagct catatcgtaa tacttttttt
    22381 gcagtctttt cgtggaccgt gatttggact ttatgtgcaa caactttgca aatcaccctt
    22441 ggtattctca cagcggttgt tgctcaccag aaatttatta aattcaaacg tattttcggt
    22501 atcattttct tattaccatg ggcggtccca gcttttatta ctatcatgag tttttcgaat
    22561 atattcaacg atagcgtagg tgccatcaat actcaagtga ttcctttcat aaaccagctt
    22621 ccttttgtag atatcagtgc aatctcttgg aaaacggacc cgaactggac gaaagtagca
    22681 attatcttag tgcagggctg gttaggattt ccctatatct atgtgatggt tacaggaatc
    22741 ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg
    22801 cagcgctttt caaacatcac gcttcctgct gtcttcctag tggcagcgcc aacatttatt
    22861 acacagtata cggggaattt caataatttc tccatgatct atctgtttaa caatggcggt
    22921 cccggaagcg taggcggagg agctggttct actgatattc ttatttcctg gatctataaa
    22981 cttacgactg gaacttctcc gcagtattct atggcttcag caatcacgtt gattatctcc
    23041 gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt caatatgggg
    23101 gattaacaat gaaaaaattt cattcgagtg ctcgattcca aaaacgactt tctcactttt
    23161 ttacttactt ttttctttta atactgtcag tcattatcat ctatccgctt ttgatcacag
    23221 caagtactgc cttcaagtca ggaaatattg cagcttttag tttagatctt tcaagtgact
    23281 ggactttagg aaactttact cggctattca gtgaaacact ttacgggact tggtataaaa
    23341 atacattgat catttctgtc ctaacaatga tcgtacaagt gactgtagta actttggcgg
    23401 gctatactta tagtcgctat cgttttatcg gccgcaaatc aagtttgatg ttctttttag
    23461 tgattcagat ggtaccgact atggcagcat tgactgcctt ttatgtcatg gctttcttat
    23521 tagaagcgct cgataagtac tggttcttga caatgatcta tataggcggt ggaattccaa
    23581 tgaatacttg gctgatgaaa ggatactttg acactgtacc aatagattta gatgaatctg
    23641 ctaaacttga tggggcaggg catttcagga tttttttcca gattgtatta ccgcttgtca
    23701 aaccgatgct cgccgttcaa gcattatggg cttttatggg accttttggg gatttcatgt
    23761 tagctaagtt cctgcttcga acacctgaaa atcagactgt tgctgtcggc ttacaaacat
    23821 ttatttctga tgtgacaaac caaagagtct ctctttttgc tgctggggca attttgattg
    23881 cactaccgat ttgtctatta ttcttctttt tacaaaagaa ctttgttacc ggcctgacat
    23941 ctggcggaac aaaaggatag gagcagagta tcatgcaaac atttccgtta aactattttt
    24001 ccgcattacg tacgccaact caagtgtttg cgggacgcaa actattatca tggcctaaat
    24061 ttttcttgat atttgtattt ttagtttcct tgatggtcat gcctgtgact cttttttatg
    24121 ccaatcagat ccaagcgatt ccattagaac aatttctatc cgttcattcg ctgatcgatg
    24181 aacaaggaac ccaaaaattt tctgaacttg aattatctga aactgggtta caagcatcgc
    24241 aacaaacgat agctgtgacc cctgaaattt tggtaggtgt gtctttatct gagaaacaac
    24301 aatccgatca tggaactttt attgattttg agaaggaaca atgggtgatc caacaaaagg
    24361 ataaatctgg tatacgccgc tatacgatga attatagccc atctttccaa ccagattctg
    24421 tacgtacgcc cgaagatttc caacgatttt tagaaagaga attttatgcc agtaatcgtc
    24481 caacaatcat tttatcttat agtttaagtc ttggtcttgt cctatttgtc atgacgagtc
    24541 tcattttatt cggggcttcc tttttcttgt ggatgacaag aaaaagccaa ctttcatcca
    24601 ttcatacatt caaagagagt gcaaatttga tgctgaatgt tatgggaata ggttccatgg
    24661 cagcagcagt tgtagggttt atccactttg actttatctt gatgctaagt gtccaaacaa
    24721 cgataactgt cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg
    24781 tcgaatgaca agtttaagcg atttatgcga caatgaatgt gaaaaaaatg aaaaataaag
    24841 tagtttttga aaaatttctg aaaaaattaa acttcttagt tgattctttt cttttttagt
    24901 attaacgtaa tagggtagtt tttttaagga ggaaaatcat gttagagaaa actttataca
    24961 atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa
    25021 cgaaacaata cggagatacg gacactcctc cccaaatcaa aatcaatatc catgaagaaa
    25081 tcccaatgaa agagattaca agtaacgctt ctttggcact aggagaagcc tatatggatc
    25141 atcgaatcga gatcgaggga agtatccaag cattgatcaa cgatgcttat cagcaagcaa
    25201 acagcttttt acgaggaaaa gattatctaa aatggctgcc aaagaaagaa aaacatacga
    25261 aacaaaaaaa caaagaagat atccatgcgc attatgactt aggtaacgac ttttataaac
    25321 tttggctaga tccaactttg acttattctt gtgcttattt caatacacct gaagatacgt
    25381 tggaacaagc acaaatcaat aaagtccatc atatcttaga caaactcttt atcaaagaag
    25441 gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt gatgttcact gctaccaaag
    25501 agtataatgt caaggcaact gggatcacac taagtgaaga acaatacgat ttgattacca
    25561 aaagaataaa agaagaacat ttagaagata aatgccgtgt attattaatg gattaccgcg
    25621 aactaaaagg cgaaacgttt gatcatatca caagtgtagg tatgttcgaa catgtaggtt
    25681 ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt attgaaacca aaaggaacag
    25741 ctttgatcca tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg atcaataaat
    25801 atattttccc tggcggttat gtccctggtg ttactgaatt agttgataat attacaaaaa
    25861 acgatttgca actgattgat ttggaaagct tacgtcgtga ctaccaattg acattagaac
    25921 attggacaaa gaattttcat caagtccaag atcaagtcac gaaagaaatg ggtgattcct
    25981 tctatcgaat gtgggatcta tatttacaag cttgtgcagc ttcatttgaa tcaagtaata
    26041 tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa tgacatccca atgcatcgtg
    26101 cataattgag gcaaaagtac tgtttaaaga ctatttaaaa agaagctaag ttttatgatg
    26161 ctccagaagt gaactttttt gtctaacttc taggattgtc tctaaagctt agtttctttt
    26221 tctttatatt ttcataataa aagtttggtt ctttaatttt taaacatga
//
LOCUS       DACDTH010000034        25501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDTH010000034 DACDTH010000000
VERSION     DACDTH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.034"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IWK54_001568"
     CDS             133..3660
                     /locus_tag="IWK54_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9593692.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IWK54_001569"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IWK54_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9593693.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IWK54_001570"
     CDS             7448..7789
                     /locus_tag="IWK54_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9593694.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IWK54_001571"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IWK54_001571"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9593695.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IWK54_001572"
     CDS             9146..11566
                     /locus_tag="IWK54_001572"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9593696.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWK54_001573"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWK54_001573"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9593697.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IWK54_001574"
     CDS             13000..13737
                     /locus_tag="IWK54_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9593698.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IWK54_001575"
     CDS             13751..14566
                     /locus_tag="IWK54_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9593699.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IWK54_001576"
     CDS             14579..15226
                     /locus_tag="IWK54_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9593700.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IWK54_001577"
     CDS             15242..15898
                     /locus_tag="IWK54_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9593701.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IWK54_001578"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IWK54_001578"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9593702.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IWK54_001579"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IWK54_001579"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9593703.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IWK54_001580"
     CDS             18240..18758
                     /locus_tag="IWK54_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9593704.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IWK54_001581"
     CDS             18865..19365
                     /locus_tag="IWK54_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9593705.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="IWK54_001582"
     CDS             complement(19645..20565)
                     /locus_tag="IWK54_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9593706.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="IWK54_001583"
     CDS             20730..21743
                     /locus_tag="IWK54_001583"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9593707.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IWK54_001584"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IWK54_001584"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9593708.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="IWK54_001585"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="IWK54_001585"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9593709.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="IWK54_001586"
     CDS             complement(23383..24144)
                     /locus_tag="IWK54_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9593710.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="IWK54_001587"
     CDS             24333..25427
                     /locus_tag="IWK54_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9593711.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5383 g         7246 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 g
//
LOCUS       DACDTH010000035        25166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDTH010000035 DACDTH010000000
VERSION     DACDTH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.035"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            510..1154
                     /locus_tag="IWK54_001588"
                     /pseudo
     CDS             510..1154
                     /locus_tag="IWK54_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            1382..2671
                     /locus_tag="IWK54_001589"
     CDS             1382..2671
                     /locus_tag="IWK54_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9593712.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            2747..3628
                     /locus_tag="IWK54_001590"
     CDS             2747..3628
                     /locus_tag="IWK54_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9593713.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            3618..4433
                     /locus_tag="IWK54_001591"
     CDS             3618..4433
                     /locus_tag="IWK54_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9593714.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            4449..5573
                     /locus_tag="IWK54_001592"
     CDS             4449..5573
                     /locus_tag="IWK54_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9593715.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            5551..6354
                     /locus_tag="IWK54_001593"
     CDS             5551..6354
                     /locus_tag="IWK54_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9593716.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            6805..7077
                     /locus_tag="IWK54_001594"
     CDS             6805..7077
                     /locus_tag="IWK54_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP9593717.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            7061..7396
                     /locus_tag="IWK54_001595"
     CDS             7061..7396
                     /locus_tag="IWK54_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9593718.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(7575..8789)
                     /locus_tag="IWK54_001596"
                     /pseudo
     CDS             complement(7575..8789)
                     /locus_tag="IWK54_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            8990..10264
                     /locus_tag="IWK54_001597"
     CDS             8990..10264
                     /locus_tag="IWK54_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9593719.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(10647..11375)
                     /locus_tag="IWK54_001598"
     CDS             complement(10647..11375)
                     /locus_tag="IWK54_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9593720.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(11353..12927)
                     /locus_tag="IWK54_001599"
     CDS             complement(11353..12927)
                     /locus_tag="IWK54_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9593721.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            13156..14094
                     /locus_tag="IWK54_001600"
     CDS             13156..14094
                     /locus_tag="IWK54_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9593722.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            14106..15017
                     /locus_tag="IWK54_001601"
     CDS             14106..15017
                     /locus_tag="IWK54_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9593723.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            15041..16513
                     /locus_tag="IWK54_001602"
     CDS             15041..16513
                     /locus_tag="IWK54_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9593724.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            16564..17175
                     /locus_tag="IWK54_001603"
     CDS             16564..17175
                     /locus_tag="IWK54_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9593725.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            17205..19118
                     /locus_tag="IWK54_001604"
     CDS             17205..19118
                     /locus_tag="IWK54_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP9593726.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            19246..22227
                     /locus_tag="IWK54_001605"
     CDS             19246..22227
                     /locus_tag="IWK54_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP9593727.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            22224..23885
                     /locus_tag="IWK54_001606"
     CDS             22224..23885
                     /locus_tag="IWK54_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP9593728.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            23925..24857
                     /locus_tag="IWK54_001607"
     CDS             23925..24857
                     /locus_tag="IWK54_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9593729.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         8107 a         4053 c         5142 g         7864 t
ORIGIN      
        1 ctgtctgtga ggacagcttt ttttgtgggc taggcatagc aaaaaggggt agtgacagtc
       61 cactgggagg gtgcacatcg accaatcgct agagaaacgc aaggtggtat ttgtgagaag
      121 atgacggaag aaatcctgta tcaaagactt ggttcaacga gcagaaatct gatagtaagt
      181 ctcggtcatt caatgggaat aagccattga acaccttgat ggtatcctgt aagaaagaac
      241 ctcaaggact ttcagcaaga agaaggaata gctgaaaaca aaaaaaggtg tagtaggtta
      301 aaagcgggga atgtagaact acttaattgg tgaaactagc tagaagggca aaatcgacga
      361 ttcatgagtt cgtaggaagg agcatgcaag atttacaact agtttactga aaaaacgcag
      421 ggaatttaca agaaattaac aagaaattaa cgcttcatcg gtatagtgga cgtgtgggag
      481 aaaatcacgg aattaaagga ggggtcaaaa tgaccatgat cactgcacac tctggtagtg
      541 acaatacgga ggagaatagc tgggcatttc ttcgcttttg tttggatcgc cagctttgtt
      601 ttgaagtaga tgtgcgtcag caaacagatg gtacgctaat actagcccat gacaggtaag
      661 aagttgtccc agattcggta acccttgagc aggtgtttca agcagtggcc aatgagaagg
      721 caaccatgac cattaactgt gatttaaaag aagagggatt ggaaatggcc gttttttcgc
      781 tggcacaaac ctatcaaatc tggccacaaa ttgttcttag tggctatgta caattatctt
      841 gggctgcgat ttggggaaag catatatttt ataatattga aaatcagttc gaagggaaaa
      901 aacttgggga tattaccgaa gcagatttga tcgatacctt acagaaatta gcctatcaaa
      961 gcgttcaaat cgtcaatatt catgaaggat ggttaactaa gttggcccgt gaagtggggc
     1021 agcagctagg attgacattt tctgtatgga cagtcaatga actagaaacg attgagtacc
     1081 ttgcgcgctg tggtgtttat aatataacct cacgttgtgc atgggcctat ttatggaaga
     1141 aaggagcaca gaataaacta ttttataatc aaaacagagg agttaagaga tttggagatg
     1201 ggatacatga ccatatgggg gctttattct tagcttgatg gatatgtcca gtgaccctgt
     1261 accccgaaat gcacaaattt cgaattattt acagaaaatt aactagaaat ttacattggt
     1321 agttcataat atttcttgat tagaaaaatg gccataaaga aattaggagg aagttaatgt
     1381 tatgttaaag aaaaatcttg ttataggggt tgtgtctgtt ttaagtgtca tctctttggc
     1441 agcttgtggt agtgggaata ccaagacaac aaaaaaatcg gagggtcgtg taacgataga
     1501 gtattggcat gttaatgcgg ataccctagg tggaaaaaca gtgaatgaat tagtagataa
     1561 atacaatcaa tctcaagaca aagtgaaggt tgtagcaaaa tataatcctg atatgtataa
     1621 aggcttgatg caaaatttac aagcttctgt tgcctccaaa gaaataccgg acgtagtaca
     1681 agtgggatgg gcttttaaag attattttgc agaaaatttc aaatatacta atcctcaatc
     1741 tttagtagaa gaagtggatc cggataatgc tgattatttt aaagatcatt ttttagacaa
     1801 tatcatggca ctgtctgaaa aagatggtaa acaagtgggt attccttact ccattagtaa
     1861 tccggtttta tatttaaatc aagatatatt aaaagcagcg ggcttggatg aaaatggtcc
     1921 aaaaacttgg gaagaagttc aaactttttc taagataata aaagaaaaaa ctggaaagta
     1981 tggcgtgtat attcaagaac ctgctgataa ttgggcacaa caagcaattt tagaaagtaa
     2041 tggggcaagt gtaataaaag atggcaaagc tacctttgct tctaaagatg gtatcgctgc
     2101 ttatgaatta tatcaaagga tggtaaaaga ccagacggca ttacacacta cgtgggaaca
     2161 aggtgtgcaa agctttattg atggtaacgt cgcgatggtc tataccacta ttgctcaaag
     2221 ggacaacatt caaaaaaata gtacttttac tgctaaagca gttgagtcac caagttggga
     2281 agggaaaaaa gttaaactac ctgctggtgg cgcaatgtta gctgttacat cacaaacgga
     2341 taagaaaaag gaagcaactt gggatttctt aaaatatctt tatagtgttg aatctttagc
     2401 taaatggaca gaaggtactg gttatttacc accacgtaaa gatgttgcag acgcccaaaa
     2461 tggattaaaa gctttcttag ctgaaaatca gatgatgata ccagcaattg atcagatgga
     2521 taagatggtc agttggagtt catttcctgg agatgctgga ttagaagctg aacaaaaatt
     2581 gttggatatg cgggataaaa ttttatcagg taattccgtc aatgaaacga tgaaagctac
     2641 tcaaaaggaa attaatgagt tactcaaata agcaaagcaa gcagacgaca gcgcctgtaa
     2701 gaaaggaaaa acaaggtcgt ctgcttttga cataaggatg aggaggctgt cggaaatgaa
     2761 gcgctatgta ggttatttgt atctggcgcc agcaattatt ttgttgggcg tgtttgtttt
     2821 ttatccattg atttatacga tctatttgag tttttttgac tggaatatgg tggcccccac
     2881 taaagaattt gttggctttg ccaactatgt aaaggtgctg actgatccag tctttcataa
     2941 agtgttgaag aatactttgt cctataccgt tctatttgtc ttgctgagct gtgtgttccc
     3001 atatattaca tcctttttat taggaatagt tatagaaaaa gggcagaatt tgtataaacc
     3061 attatttttt attccagctg tgatttcttt ggtagtggcg gggatggttt ttacatggat
     3121 attaaatccg gtatcgggac caattgctgt cttgttggga aattttggtt taagactgcc
     3181 tatgtggatg aatttacaag gttgggtaat tgctgtgatt gcaggtatta ctgcttggaa
     3241 agtttttggt tataatttca ttgtcatttt tgcagcaatt gtgggaattg atcaagagat
     3301 gatcgaagca gctaagttgg atcgtgtacc catctggcgc atttttttta caattgtgtt
     3361 gcctatgagt agttcgacag gactatattt attggtgatg accattgttc aaggtctgca
     3421 gtatgtgttt actccgatta agatcatcac tcgtggtgga ccgaataatg gtagtgcaaa
     3481 cgtgatctat tatgcttatc aaaaggcttt tgaaatgtat aaagtcggag aatcttcagc
     3541 aatttcagtt ttaactttat taatatttgc tgttttctta ggggtaatat ttaaatttgt
     3601 ggaaaagggg gtttactatg aaaattaaac gctggttgcc acatctctta tttatgttca
     3661 tcataatcat tattatctat ccattattgt ttattatggc caattctttt aagccattga
     3721 aagatgccta tcagacgatt actagtttga taccacagcc gtttaccttg gaaaattttt
     3781 gctatttgtt cgaaaatctt ccaattatca aaattacgag aaatactttt tttattgcta
     3841 caattataac ggttgttaag ttaggcgtag ctttttttgc ggcatatagt tttacttatt
     3901 gccgaatcaa agggaagaaa atctggtact ttatcatcat tggtacgatg ttcattcctt
     3961 ttactgttgc gatgattcct aattatttgt tgattgcaaa acttaattta attgatacta
     4021 cttggggtgt gataatacca caatttgctg atgcgatggg cattttttta ttgacgcaaa
     4081 caatgcggga aattccagtg agccttctag atgtggcggc attggataac atcaaacaaa
     4141 caaccgtcat gagtaaaatt gtttttcctc ttactagaca tgcgattact tctaccggta
     4201 tttggttttt tattacctct tggaatgagt atgtttggcc ggtattaatt ttacggacag
     4261 tagataatta tactttgccg ttggcgatgc agacctatat tagttctgaa ggtgggacaa
     4321 attttaccgt agcaatggcg attagtttaa ttacaatgtt tgtaccgtta gtcttgtatg
     4381 caatttttca aaaatatatt atcgggactt ttgtttctgc aggtattaaa taaattggag
     4441 gacaaatcat gggagaaacg cttgaattaa gaaatatcgg aaaaaaatat ggagaacata
     4501 ccattttaaa agaaattgat ttagctgtgg ccgctggaga acgaattgtg ttactgggac
     4561 catctggttc aggaaaatct actttgctga gaatgattgc tggtttggaa aaaattacga
     4621 gtggagagct ttttttaggt acacaactag caaataagct accttgtgga gaacgagaga
     4681 tcgcaatggt ttttcaaaat tatgctttat atcctcacat gactgtaaag gagaatatta
     4741 tttttgcatt aagggcgaat aaaattaaga cagctgacat tcagcaccgt ttggaagaag
     4801 ctttgaagat gttaggttta acgccatacc aagatcgact tcctaaagat ttgtcaggag
     4861 ggcaacggca gcgtgtagca ttggctcgtg cggtggttaa acggagtaat tattttttat
     4921 tagatgaacc gttatcaaat ttggatgtgc gtttacgatt ggatgcacga aaagaattgg
     4981 taaaaattca tcaacgttat aaacaaactt ttgtctacgt cactcatgat caaattgaag
     5041 cgatgacgtt agctgaccgg attgttttat tgaatgaagg tagaattcag atgattgata
     5101 cgccgaaaaa tgtttatacg aaacctaaaa atacttttac tgcaaatttt attggttcgc
     5161 caggcatgaa tattatgcca gttacttatg atcaaggaac attaaaggta gccagtcaat
     5221 ttttaacatt gaatccagct tggcaaaaac aacttcttca ggttagtggc tcattgtatt
     5281 taggcataag accagaacac gtaacctttt caaaagaaac aagcgattta cacggtcaag
     5341 tgaagtatcg tgagctatta ggtcaaagtt atgcgtacac cgttaaagtc gatggtatag
     5401 aaatgattgt ccttaatgaa aaacctactt ggcagattgg tgatgcagtc aatctccgca
     5461 tagaacctga aagaatgcat ttttttgatt atcagacgca agaaaattta ggatatcctg
     5521 attattttat tgaagaaaga gggaatagcc atgcgctttt tgcatctctc tgatatccat
     5581 tttagagaaa actatcaaac taacttgttt actgctgaac tatttccaac aattaaaaat
     5641 ccacagttgg cattggaaaa attgttacaa gaactctcgt taacagtgta cgattttatt
     5701 ctattaacag gtgatttagt acatgaaggg gggatttctg attatcaagt cttacggaaa
     5761 atgttggatc aatatttccc aaccacacct tactattttt gtcgtgggaa tcatgatcgt
     5821 cgcagtgttt tctttgaagg aatgagtgtt gaaagcaatg aaacgggaga gtatatccat
     5881 tgtacgaatc aaaatgggtt acgaattatt tccttggatt ctgctcaaga tacccagcac
     5941 gaaggaaaaa tatcgatagg acagatgatt caattacaag agtggttggg tacacctgct
     6001 gaaaagggaa cgattttatt ggttcatcat ccattgtcgt gggaagatcc atctattgta
     6061 actgaagttc cggaaggttt tgctgaattg attgccgaaa gtgatatttt aggtattttt
     6121 gtaggacata tccatcaagg tagcagtgct gaatttgctg gtaaaaaaca gtttatggct
     6181 gaatctttat catttggtgt agatgaatac ccagactatt ctctttttac caatagaaca
     6241 ggattcacta gtggcagttt aattgacgga aaaattttta cttatcagca ttatttaact
     6301 ccacatcaaa tagaaatagg gcgcatgaaa aaaccaatta ctggtaatca ttaaaaatta
     6361 ttgattaaaa ataaagactt cattcacttt ttagccgtgg tgctcaatgt ctggttccac
     6421 ttgaagaact tggaaaatcg ctatcaataa accactatcc ctccaattac aaaaagggtg
     6481 gagaaagttt tccaagaacc atatcggtct tcgtggttgt gccattcgtt ttttaccaga
     6541 gcataccaca tctttgtgga gttttcagac acgtcctagt taatttggtt cttttaggag
     6601 tcttttcctg ttctgtgtgt cgttgagtgt tcgcttcaat tatcgctgtg gcggattttg
     6661 aaacttttct atctatcaag gccatttagc actatatcct ttccttatat ttagataaat
     6721 acactgcatt tcaaggagtt caaaagtagt tgtattaatt ccatttgaac tctttccgtg
     6781 atataatgtg tttgtcagaa agaggtggag aaaatggcta agttacaaat ttctatggac
     6841 gaaaaattga aaaatgaagc agaaagcatt attgatgaac taggcatgag ccccaaaaca
     6901 gccgtcaccg ttttttataa gcaaattgtt gagcaaggaa aaatcccatt ttctattgaa
     6961 ttgtcggaaa aaagtaaacg aatccataat attcaaagat tagcggaaaa attgcctgtc
     7021 caaaaactag acactgacga aaaaattgaa gaatggttca atgaagatga atactaatga
     7081 tatagcaacc gtctacgttg ctttcacagc tgcagagggc ggtaaacgcc ggcctgtcta
     7141 tattctaaaa aatggactgg acacaatttc gtttctgtca attacttcaa aatatcatac
     7201 taaatccgat aaaataaagc tgaaatacgt tgaaataagg gattggaaac aggcaggact
     7261 aaaaaaagaa tcttggattg acgtaggaac tctaaacgaa ttacccaaaa acaacaaaaa
     7321 ggtcaaatgg aaaaaaattg gggaattaag tccaagcgat ttacagcgac tggaagaacg
     7381 catggaagaa ttataataat actaaaaaga agggctcttt gtcaataagg actgatgggt
     7441 tgtacaaaat caaatctggg tcagaatgaa cctcattctg gctcagattt ttggctcttt
     7501 gtcaataagg actgatgagt tgtgcaaaat caaatccggg tcagaatgaa ccacattctg
     7561 gttcagattt tttgttatct tacattgatt aattgattga tcaaaaagat tctaattctc
     7621 atgttctcga atgatttaaa tccgtaggat actcgtttca ttgtctttat gtgggtattc
     7681 ttcgcttcaa tttttccatt ggaataagga tagatcattg cgctggtgat accttcttca
     7741 taggtcagaa ggttttgaag cttctcccga aagccgtcat ctaacgtttc gggaagttct
     7801 gccaataagg agaaaaataa gtcagggtct ttgtctcgaa aggcttcgac taattcatga
     7861 aaaaagggat acgcctcctt tagaggcgca gaaaactcaa gcaatcgatc aatcatcatt
     7921 gcttcagtga gaaatgggta ttttggtgct cggaaacttt tccatgtttt gtactcataa
     7981 tggttgatgc ttgcacgatt ttttagcaga aagcgccagt tctttttcag tttttctgcc
     8041 tgacttttct gtcctgcttt acgaagctca ttcatttcac ggatacgcaa ctcattgagt
     8101 gcctgattca tgtgtttgac aatatgaaac cgatcaatca ctactttcgc atttggcaga
     8161 acacgtttgg taagctggaa gtaagcggcg ttcatgtctg tgactaagaa ttctacttct
     8221 tctggattgg tgcagcctaa gaaatagctt gttaatcgag gtaatttacg cgtaggcaag
     8281 acagcaatta agtttcctgt ttcaccatct gcgcaaataa agctcatttt atcttctatg
     8341 gaagcgtgcg aacgaaattc atcaaccatc aatactctgg ggagaatctt cttagaaggc
     8401 tttggtaaat agcttttaaa ctctttcaat gtacgagtaa cggtagtcaa agatacctga
     8461 caacttttcg caataaaaga caaagatact ttttcagtca gtaaagaagc aattttatat
     8521 cgaacatgat ctgcgattga atggctgagt cggacaaaat aactttgagc cgtccaatgg
     8581 gtacgacagt ttttacaggt atagcgttgc ttttttaggc gcgtaaccaa aggcatatga
     8641 ttgtattgtt caaaacggac aatcgtttcc ttcttcccat ttttcactat aattgctttc
     8701 ccgtttccat ctaccacagt agaaccacaa cttctacaag cacgaggagt aggcgagaga
     8761 acagcatcga cgaccaacgt ctttttcttg gctctttgtc aaataggact gatagagtga
     8821 tataagaaca tctaaggtag aattgatttt gcttctataa tattttgtgt tggtggaaaa
     8881 tcgaaggcga ttttctacta acactttttc ttcgtttcaa ctcaaaaaga gactcacttt
     8941 tggtaaaatt tagtcgacca aaacgtcatc ctaccaaagg agtctctcaa tggataatca
     9001 tactagaaaa ttactcggat taacagataa acatttattt tttgatgaag aatggctgat
     9061 tgaaaaagaa tataaaggcg ttcaagcgca gtttattaaa ggaaaattgg atgactctcc
     9121 ttcctattgt gaacactgtc gatcggttcg tattatccgc aacggttcct atacgacacg
     9181 cactcagata ttgaaagtca aagaaaagtt aactatttta gaattaaaac gtacccgttt
     9241 tctctgccat gattgtggaa ggaccttttc tgcgaaaacg gacttagttg atgaacatca
     9301 tcaactaaca aaggaactga agcaggcaat tctcatggaa ctgtacgaaa atcaatcacg
     9361 taaactaatc gctaagaagt acttcgtctc ggatgggacg gtgacacgga tcttgcgtga
     9421 agcaacgaag cattaccagt cacgtatgaa cttccttccg acggtgttgt gtatggatga
     9481 gttcaaatca atgaagtctg tttctggttc catgagtttt atttgtgttg atgggacaac
     9541 ccatcagcta tttacgatcc ttgaagatcg cagactttac aaactaactc aatacttcct
     9601 gcactttcct agaaaagccc gtttaaaggt caagtaccta gtgatggaca tgaatgccag
     9661 ctattgtcag ttgcttaaaa cagtctttcc aaatgcggag atcgtaactg accgctttca
     9721 tattgtccaa cacatcaatc gcagcttcaa tcaattacgt gtccagatca tgaatcgttt
     9781 tcgaacctca cactctgaaa atcagaagaa gtaccgtcgg ctcaagcgat attggaaact
     9841 cttactcaaa gattccacga catgggagcc actcaaacgg cattatcatc gactgtttaa
     9901 acgaccaatt agtcaaacag aaatcgttga tgagctgtta agctacaacg aagaattgcg
     9961 aactgcgtat catttttgtc aattattacg ctattatttc gttaaacgag gtacggaagg
    10021 ctttcaagaa acccttaaaa caatttcatc tacgttacct cagtcgttta aaaagaaatt
    10081 cacgatcttc cattgctacc aatcgggtat ttcaaatgcc tttaaggtgt cccactcaaa
    10141 cggcgtcact gaggggctga ataacaagat caagctgatt aaacgaattg cgtttggtta
    10201 tcgcaacttt tataactttc gggcacggat ctatttacaa caaggtctga tctttgaaaa
    10261 ctaaaaaaat atgcggcgca cagaacgcgc cgcatatcgg actaagtttt attcaaattt
    10321 gtcttcacca acacgatttg acgaagagcc tatttaaata actacatcca tttattctat
    10381 cttctaaaat agcctttttc atttcagtta aatagttcag cgccattttt gcactcgact
    10441 tgcatagtag ttttttccat attcctcaac ctcagcgggg gttgaagaca taaggaaaat
    10501 cctatgcgtt atgctcaact ggatctgatt aaatattcta atgtattttt ctctaccatc
    10561 attaaatggt taatagccaa aaatcaccac ttttcaatgg ctccttgttg tataagtata
    10621 actagaagat gaacacgtct gagaaattat agaagctttt tacggtaatt actggggctg
    10681 tgtccagtag tttttttgaa gagaacagaa aaatagctca cgtgatctat tcctactttt
    10741 tgactaattt catcaataga taagaatgtg tctttcagta gagcacttgc tcttttgatt
    10801 cgaaagtcta gcaagtaatc atgaaaggaa taacctgttt cttttttgaa ggcccgtcct
    10861 aaataagcgg gatggtaatg gaattgtgta gctacatctt tgagcgtaag cttttcacta
    10921 taattgtttt gaacgtaatc aatcacttgt tgaattgact cattaaaaga atcatcagtt
    10981 agagagtttt gattagctgc tgatttacgc attttttctt cttgtactaa cttaactaaa
    11041 taaggaaaat caatgggttt cgttatgtaa cttttaatag ataaatcaat cccctttttt
    11101 gcatactgaa aattttggtg ggcactaatg acaatactag taaagtagcg attggatgtt
    11161 tgttgcaaag attcgattaa ttcgagtcca gaaattttcc ccatcgtaat atctgtgata
    11221 agtaaatcaa agcgctggtt ttgcaggttt ttaaatgctt cgtctccgtc tgtgtagcag
    11281 tgatcgataa tgatgtcggg gactttcttc aaaagataat gccttatgcc ttctacaatt
    11341 gtacaatcat cgtcaacaat tgcaattctc aacatgcata ttcccccctt tttgtatttt
    11401 gatagtaatc gttgttccga cgtgtagttg gctttctata acaaaacgtg tatctccagg
    11461 atagagatgc tttatacgtc gaaaagaatt tgagatgccg atattctcac tataaggaat
    11521 atcatcatta agtttttgtt gaatatgctc cagcttgtct tggtccattc ctttcccatt
    11581 ttcaacgatt tttaacacat aatggttggt tgtttcagtt gcatagacag tcaaacgatt
    11641 ttcctttttt gatgaatcga agccgtgttt aatataattt tcaacaaatg gctgtaaaga
    11701 aagttttggt aacattaatt gatcaatatg atcaatcagc tgatagtcaa agttatgtcg
    11761 ataacgacgt tgatgtagtt gcaaggagag gtcgatatta attaattcct ctttcaaatc
    11821 aattatgtcg ggctgatatg tattattgcg atacaacttg ctcaataaat atatactgtc
    11881 gctgatttct tcgtcatcat tcaatatggc agtcattcga ataaattcca aattattaaa
    11941 tagaaaatga ggctcgattt gatattgaag tgctgcatac tttgcttctt gcttcaacca
    12001 attattttca taaagtgttt tttgatatgt agcattatct aaataaaact gattgatatg
    12061 catagcgatt tctttcaaat caccgacttt gttttcgata ttgattgact tgtagtctcc
    12121 tgtagatgaa tgatttaagg aagtaatgat ttcctgatac tgctggatat agttacgaaa
    12181 acttctattt aataattgat gaatagcaag aataaacaaa ctcattaata agaaaatcat
    12241 gattgccaga cctaataagg tttgtgacga ataatttgga ttttcatata cttgtaatgt
    12301 gtggttaccg atagctatct gttgcgtttt tttccattta gaaggcgaga tgtcttcaga
    12361 taaattgacg gaatcaatac cattagacaa agaataattg taatgtgttg gaaagagact
    12421 cttttttagc gcgttgatcg aaacgtctag aatcaatgta gcatcgggca tgtcctcact
    12481 aatgaaactt aatgaacgaa taaaagaaat tttttcgttt tttgtaggtg cgtaggcact
    12541 taagtatttt tcgtaatcta atgagataga tatattttta acttcatcat cttgcacaag
    12601 tttttttaag ttatctacta aataggtagg ggagcgatcc tttgacaaaa tatcttccat
    12661 gtattcagca ggtgatgaga ggtagtagtc attaaggttt tcttggaaat cataattttg
    12721 atagatctct gtcacgatac cggagagaat acgattatat gtttccatat agtgggtgat
    12781 ttgctgaatg ttttgcaggt tttgattttt ttctttttca atcacattga tataggaaaa
    12841 gaacaatgtg aaacaggcaa gtaaagtaat aaaaaaaatc aatatgtatg taaaacgcat
    12901 aataactttt gataaatcgt ttttcattca aagtcctcat tttgataatc tctaatttta
    12961 tgaatcatta tagcacatat tacaagtgat gtaaacgctt tgcaaacagg gctacttatg
    13021 attgtgaata cagtttccgt ttaaaaacta ttctattgta gaggtttaat aaactaaaaa
    13081 taatgtataa aaaattaatg taagcgatta accaagtggg tataatcaag gaaacgctga
    13141 gagaggagag acaaaatgaa gctaaagaaa gggttattag gaaatctaaa aaaaaaccga
    13201 gcattgttta ttatgctttt accaggtgcg ttatggttat ttatattttg ctatatacca
    13261 atgtttggag ttgtaatcgc ttttaaagat ttcagaattt catcaagggg attctttaca
    13321 agtttgttca acagtccttg ggttggctgg gaaaatttca aatatctctt tgcttcgcaa
    13381 gatgcttgga tcattactcg caatacggtt gtttataacg ttgtaggtat tttgctaagt
    13441 actttttgta cagtgtcatt agcaatcatt ctgagccaaa tggcgaacaa acggttattg
    13501 aaaaagatgc agacatttat gatttttcct aattttttat catgggtagt catcagttac
    13561 tttgtttacg catttttgag tacggataaa ggattaatta atattttact aagcaatttt
    13621 ggtgtggaac ccataaattg gtatagtgaa ccgaagtact ggccttttat tattgttttt
    13681 acgagtttat ggaaaggcgt aggctatgga tccatcattt attacgcgtc aattatgggg
    13741 atcgatacta gttattatga agctgcttta gttgatggtg ccagtaaatg gcaacagata
    13801 aggaatgtag tcttgccaca tttaagatcg attattgtca ttatgttgat tttgtctgtt
    13861 ggtagtattt ttcgagctga ttttggattg ttttatcaat tgccaagaaa ttcgggggct
    13921 ctttatgatg tgacgaatgt attagatact tatgtctacc gtggattaat gaatagcgga
    13981 gatatcggaa tgtctacggc agcgggatta tatcagtcat cagttggctt tgtcttgatt
    14041 ttgattacta ataagatagt caacaagtta gatcctagtt cagcactatt ttaggaggaa
    14101 tcaaaatgat atcaaaatta aaacggcaga tgtacttttc gctgccagtc gaacggtttt
    14161 tcacaatatt actaatctta tttactctta gctgtatttt tccctttatt ttcgtaatcg
    14221 tcatttcatt aacagatgaa aaagcattac aactcaacgg ttacgaatta ttgccggctc
    14281 aatggagcac tgatgcatat caatacttaa tacaagatgg cggacagtta cttcggtcat
    14341 taggcgttac aattatgatt acagtgattg gaacgttgat tactgttttt atgacgggaa
    14401 cctatgctta tgttttatca agagcctctt tcccttaccg gaagttcttt actttttatc
    14461 ttttttttac catgttattt gcgggaggga tggttccttc ttacttagtg atgacgaaga
    14521 tgcttggctt aaaaaataca atttgggctt tgattctacc tcttgctttt agcccataca
    14581 atgtcattat tttaaggaca ttctttaaga agtcgattcc agaatcgatt atcgaatccg
    14641 ctaaaatgga tggctgtagt gagtttcgag tttttttcca aatcgttttg cctttagcga
    14701 tcccaggggt tgcaacaata ggtttatttt caagcttagg ttattggaat gactggttca
    14761 acgcattgct gtatattgac accaacaaat tgattccatt gcaatatttg ttaatgaaga
    14821 ttcaaaattc aatggaattc ttagcaaata ataatgacat tacattagcg cagcaacaag
    14881 caattcaaaa tagccttcct caagaatcaa cacgaatggc aatggtcgta gtagctactt
    14941 taccgattgc aattgtttat ccgttcttcc aaaaatactt tgttcaagga ttgacgatcg
    15001 gtggagtgaa agaataaaaa agaataggag atgttaaaag atgaaaaaga acatgcagaa
    15061 atatttgatt ggtggtgctt ctgtactaac ccttggccta ttaggtgctt gtggtagcag
    15121 taatggcgaa gcaaataatg aaacaggaac cacaaaagtt acttggtatc aggttggaga
    15181 agaacccaaa gattttgatg aggtcatgac aaaagtcaac gaaaaattaa aggaagaaat
    15241 caatgtagag ttggatatgc ggctgattcc ggacggggat tatcagcaaa agcttggtgt
    15301 gatgatcaat tcaggagaag aatatgatat ttgtttcgtc aatggcacag attatgtgaa
    15361 ttatgggaac aaaggtgctt tcattagtct caacgatcat ttggataaag agttgaaaga
    15421 ttatgcagct gaattgaatg aaggatttat tgaaggtggg gcaattaatg gcgaaactta
    15481 tggattgcca gtcaataacg atattgccac aacggaatat tggaccttta ataaagattt
    15541 ggtagatcaa taccaattgg atgtagagaa tgttgaaggt gtagaaacat tggctccagt
    15601 attagaaaag tttgcagaaa aaaatactga tacgaatatt acgccaatcg gtatgacccg
    15661 taccagtgtc tgtattcctt atgacaatat tatcggtggt ggtgtaccgc tagcggttgc
    15721 atttgaaggc gatacacaaa cagttgttaa tacttatgag actccagaaa tgatgactta
    15781 tttgaaggct atgcatgagt tttataaaaa aggatatatt ttgaaagatg cagccactgt
    15841 tacgacagat ccatttcgaa ttgatagtaa aaactggttt ttaggtcgta actcaagtgg
    15901 ttatcaacca ttagcacaaa aaaaattaga agctctagcg ggacatggat taattttaaa
    15961 accagctgga aaagctttta aaacgactgg atttgcaaga gtggcaatgc atgcggtatc
    16021 tgcttcaagt aaacatccgg aagcagcctt gaaattgttg aattatgtga ataccaatca
    16081 agaagtagcg gatttaatgg tttatggaat tgaaggaaaa cactatgatc gcaatgaaga
    16141 tggttcgatt actttaacaa actctgatga ttattatcaa ggcgcttggg cattggtaaa
    16201 tacttatcta gtttcaaaaa cttcaccgat tacagaagaa agtaaaaaac tagaagaaga
    16261 aagtaaaaag tttcaaaatg aagcaccgat gtctcctatt ttaggtttct cttttgacgc
    16321 ttctgatttt cgaacagaaa ttgcaagtgt acaaaatatc gtgtcacaat atgaagtact
    16381 tttaagctcg ggaacagttg accctgaaca atatgtacca gagttgttag aaaaactaga
    16441 aaaagctgga ttggataaaa ttcaggcatc tgctcaagaa caatacgatg aatggcgaaa
    16501 atctaacaaa taatttttta ctaaatgaaa agaatagact aagcagttag tggggggatt
    16561 caaatgaacg aaaacaaatg gaccaaagga tttaattggg tttttcaatt gttgttacta
    16621 aactttactt attttatact aatttgttta ggatttggaa ttttaggttt tttccctgct
    16681 acgatagctt tgattgctgg cgttgtgaat cgaaaaaaat atggcttgca agagtctgtg
    16741 aagttcatga gtaaaatcta taagaaaaat tttgttagat ataatctata ttgctggtca
    16801 gcaactttgt tgttggccgg caatattact acgatctggc tttgttggca gcaaagcgca
    16861 ccattgtttt atttattaag tacattatta tcggtaatta cactgttcct agtctttgca
    16921 atgttgaatt gggtcgtgat caaaagtctt gggttgtgga tgatgggaga ccgactgatt
    16981 gactatttgc ttttcacagc aacgatgttg ccaacggtag tcatgcaagg cttggtgctt
    17041 ggaatttatt tccgaatatt gatgattttg cctgtgattt ttctgtttgg cggaacagct
    17101 gtaacggtaa tattgattag taaattaact gaaagagcgt tagcaaagtt gccttgtcag
    17161 atacaatcgc actaattttt tcacaaaaag gagtatttct ttctatgaag ttaactgata
    17221 aacaagtagc taatatagcc tttatccagc aacttgagat tccattgaca caaaaagatg
    17281 aagaacaaat agacttgatt tttactgaca caaaggctaa tgtgcgacaa atcaagatta
    17341 ctaaaaatga gcagcaacga atttgcttgt cttattcaca tgaagacttg ctgtatcgag
    17401 gattgtttat ctgccttcca cgttttttag ctcaaggagg agagatgcca tacattgaag
    17461 aaacctcgat cattgagacc agttattcat tagatgcttc tcgtaatggc gtgatgaata
    17521 aagagactct aaaaaaattc atttcttact tagcgttgat ggggtacaac acattgtata
    17581 tttacacaga agatacctat gaagtagaag accttccata ttttggttac cttcgcggag
    17641 cgtatacaaa agaagaagtg aatgaaattg ttgaatttgc ttctggtttt ggcatagaag
    17701 tagttccatc gatccagacc cttgctcact tgacccaatt tttgaagtgg tatgctcaac
    17761 aagaactgat ggatgatcaa aatactctac tgatcggtga agaaaaggtt tatgcggcaa
    17821 tcgaaaagat gattattgcc tgcaaagaaa tgtatcagac agagcgtatt catttgggaa
    17881 tggatgaagc ctatcaagca ggtctgggac gatatttaag cctgtatggg tattgtgatc
    17941 ggacaacttt aatgattcaa catatggacc gtgtgttatc aattgttgat aaatataaga
    18001 tgaaaccaat gatttggagt gatttcattt ataagctatt agatgaaacc ggtaaagata
    18061 ggtattacaa tcctgatttt aaactcaaag cattagcagc taaaggatta ccagcaggat
    18121 taacctatgt tcattgggac tatggttcag atgaagtttc ctcctatgag aaagtaattg
    18181 ataaccattt gaaattttgt gacattgatc attacgtttt agctagtggt gcccatatct
    18241 gggggagact agccccgaat catgggaaaa cagaagcaat tgtttctgca gctgtaaaag
    18301 cttctaaaaa taaaggtgta cgttctgtga tgttaactac ttggggagat gaagggcagg
    18361 aaacagagca ttggcattcc ttaattgggg cattaaaatt agcagattat gtctattctg
    18421 aagaagagtg gaatgatttt gaggaaatct acgatcgttt attaggaaca ggtagttatc
    18481 aattaatgtc cgaattacga tatttcgatg aagtggatac cattacaaat aacaacttac
    18541 aaatggcttc cgtgagtaaa ctgctacttt ggcaagatcc gttagttggt atatatgact
    18601 ttcatgtagc cgctcataac aaaattgcca ctagaacaat tggtacttat tatcatgagt
    18661 tagctcagaa attagatatg aataaaaatt ggaatccaaa acaaccaatt ttaacaacta
    18721 ttcgtcaacg atatatactt ttagctgaaa ttttagcaca aaaggcagaa ctaggcatct
    18781 atttacaaca ctgctgtctg gtagaagacc ttgcaggatt aatggaatta gcgaatcggt
    18841 gtgaagcact agccaatcga gtaaatgaat tggcatctat tcatgaaata atttggtatc
    18901 aaacatacaa aacctatggt tgggaagtat tagaacaacg atatgctgca ttagctcatc
    18961 gtctgaaaac cgttgcgcgt cgtattaaaa atcatcttca ggggcaagat gccctagagg
    19021 agcttttgga agaacgatta ccgttttgtc aatatcaagt gccgcttgaa atttctggct
    19081 ttaattatcg gcaaagctcg gtgtcaggct acaattaacg aaggaatctt cccgttgaac
    19141 ggaccacttg ctcatctaat gggaaagtgg ttcgttttct attcataagt agcttaccac
    19201 taactctttt attaagaaga gaaaaaaagt aggaagtgag aaaatatgtt atcggactat
    19261 tatcaaaatc tgtcagttat aagtgtcaat acagaaccat tacgtaatta ttatattcct
    19321 tatgcaagta aagaagaagc aaaacaagag ttagaaaggg aaaaaacgag ccagtttatc
    19381 gatctcaatg gtacatggga ttttcagtat tatccttctg tccaacagtt accggaaaat
    19441 ttttttcaag aggcctttac tgaaagctat gatagaattt ctgttcctgg agtttggcaa
    19501 acagagggat ttgacttacc tcagtatatc aatgtatctt atccaattcc ttttgatccg
    19561 ccttatgtac ctaatgatac cccttgtggt gtatatcggc gtcagtttca aattgagcaa
    19621 acagatgcac gcttttactt aaatctagaa ggagcagata gtaattgttt tgtttggata
    19681 aatagaaact ttgtaggcta tgaccaagtg agtcatagta catctgaatt tgatgtgacc
    19741 aagtatttac aggttggggt taatgaaatt caaattctag tgatgaagtg gtgtgatggt
    19801 acctactttg aagatcagga taagtggcga atgagcggtc tatttcgtcc agtgtatctt
    19861 cttcaacgac caactatgca tctgacaagt tatcaactac ggacgttgac tgatcctaaa
    19921 aaaggaagcg gaacattaat ggtttctttt caacgattta ctcctattga agtatcgtta
    19981 cctatttctt acgaattatt ccatcctgat ggacgtctca tagcgaaagg ggagtgttac
    20041 tcggaacaac aaataaattt ggatgttgaa gattgccttt tttggacagc agaaacgcct
    20101 aaattatata cattactttt aacatgtaat ggtgaatatt ttaaagaaaa agttggcttt
    20161 cgagcactga cgatcaagaa tcgtaagtta ttgctgaacg gaacacctat taaattatat
    20221 ggagtcaact atcatgacgt gaatcagcaa ggcacgccag tgatgacccg agagcagtta
    20281 cgtgcagatt tgttattaat gaaaaaatta aatgtgaata ctgtacgaac ggcacattac
    20341 cctaaatcac cagattttta tgaattggcg gatgagattg gtctgtatgt aatcaatgaa
    20401 gcagatttgg agtgtcatgg tgtggttaat ctatatgata ctgaagcgaa ttatcaacta
    20461 attgctaatg atccgacatt cgaaaagccg attatggacc gagtgatccg ttctattctt
    20521 ccttatcaaa atcgaagctc tatcattatg tggtctatgg ggaatgaatc tggttatgga
    20581 gtaaattttg aaaaagctca agctttagca cggaaattgg atgattcacg gttgattcat
    20641 aatgaacgct cgattgaacc agttattgac caagtgaatg attttagtaa cttggatgtt
    20701 attagtcaaa tgtatccccc actggaagat gttcatcgtc attgtcaaag aagtgatact
    20761 gaaaaacctt tttttatgtg tgaatatagc cacgctatgg gcaacggtcc tggagatctt
    20821 aaaacgtact tcgatttgat tcaacagtat gattcctttc ttggcggttg tgtctgggag
    20881 tggactgatc atgcaatcaa tagaggaact ttagaaacgc catcttattt atacggtggt
    20941 gattttggtg aaaaatatca cgacggcaat ttttgcgtag atggtttatt gaccccagat
    21001 cggcaattaa cagtaggtgc attggagtac cgtaatattc attcgcctat tcaactgatt
    21061 gctgtagatg aggagctgca agaagtaacc ttgagcaata taactgcttt tacagccggt
    21121 tcagcattta acctttctct tgaatggtat atagatggaa aaaaaacagt ttatgaagta
    21181 atgaatcctt tcatacttga gcctggcaaa agttgttcat tgcccatcac agtacctaca
    21241 acgattcatg gtgctcatac ggttgctgtt tcgctatctg tttataaaaa tgatgagctt
    21301 tatggacagc aacagctctt tcttaaaaaa gaaaaaatga gattgccaag aactgctcat
    21361 cataatgatg caccacaaat ttcagtaaaa ggcacagagc tgataatcaa aggagaatcc
    21421 tttatttata tatatcaatt agcaaaagct gcgttcactt ctgtaaagat caatcaaaag
    21481 gaacttttgg aacgtcctac tcaattttca atttggcgtg caccaacgga taatgatcgt
    21541 aacttagtaa acaaatggaa agcagctgga tatgctgaaa tgtctttaga aatcagaaaa
    21601 aaagactgga aattcgtaga tgatcatctc ctgatcgaaa cgtcttatcg aggagttatt
    21661 ccaggattac agccaatttt tacaatgcac atacagttga aaataaatgg tgcaggcgta
    21721 atcaaccaaa aagtatcagt ggagaaaaat ccgatttttc cagatttccc tcgctttggg
    21781 atggactggc cgttgaatga tactttttcg aaggtttctt attgtggcta tgggccgtat
    21841 gaaagttatc ctgataagca ctatgctgcg atgtttggta tttatgagat ggagattgag
    21901 gaatttgatc agccatatat caaaccacaa gaatatggta accgctggca atgtgattgg
    21961 gtatcaatca aaaatcatca aggatctacc atgacggttt atggaaactc taatttttct
    22021 gctctgcctt atagtcaagc tcaattaacc gaaaagtcac accattatga attaataaaa
    22081 gaaaatagaa cttggttcaa tttaagttat aaacaaaatg gtatcggctc acacagttgc
    22141 ggtccttatt tagaagatcg tttccgtttt atggaagata cttttgactg gaatatagtg
    22201 attactttca aaggagaaaa tagatgaaag aaacaaagat caatcaagtc aaaaaaaaat
    22261 tatctcgtta tcaagaagtt gtttatcgaa accattgctt aaaatggtca aaaaataagc
    22321 tgaatgttcg ttttaatgga ttattttatc gtttcgaaca gttagaagaa gtctggaaac
    22381 cggtaacgaa tttccaatca tgtcctgtac agctcataaa tgatccaaaa gaaacatacg
    22441 atttatgtat aacacacctt tataaccagc atttgaaaaa tgacggattt tatttaaagg
    22501 ttactaatga caatcaactt acgattgaat cagcaaacct tcgagggttt caatatgcta
    22561 tggaagcctt attagagagt tttttttcgg taggagatca attattatta ccggaattga
    22621 ttctgaaaca tgaaccatca gtgaaaattc gaggaatcat tgaagggttt tatggtatcc
    22681 cctggacaca tgaaatgcgc ctctctcttt tttcatatat gaaagataac caattgaata
    22741 cttttatgta tgcccctaaa gatgatgaat tattacgtaa aaaatggcgt gaactctacg
    22801 atgcgcaaga attagacaaa tttaaggagc ttttatctgc tgctgaggct agcaatatcg
    22861 atttttggta cttaattagt cctggaaatg atattgatat tacgtgtgaa gaagacattc
    22921 aagttcttct gaaaaagtta gaacaactga ttgagcttgg cgtatttcaa tttggtctat
    22981 taatggatga tattgactat caattaaaag gtgctgctaa acgccgtttt cgagagccag
    23041 cattcgctca tgcctattta gtgaatcgag tagaggaata tcttagcact cggttgtcca
    23101 atcatgaatt agtcatttgt cctacagaat atgataatcg tcatggttct cgctatttag
    23161 caacgttaag tcaagaaata ccagaacaat taccgttctt ttggactggc ccaagcacgt
    23221 tagcggcgtc aatttctaca gaagagctgc aggaaatggc ttctgtctat caacggccga
    23281 tgctgatttg ggataatatt ccggtaaatg attatttaga ggataaagaa ctattgttca
    23341 tgagtccata tgagaatcga acaccgaatc tttctaagga gagataccag gttacaggag
    23401 tagtttcgaa tccaatggct caattagaag cttcaaagtt tacgatcaat agtatggcta
    23461 attacttatg gaactgtgag cgttttgatc ctttggaaac ttggacgtca gtcgttgaaa
    23521 aagtagttgg aagtaaacta caaccagctt atcttacctt aaccaatgct tttccaaatc
    23581 attacacaag cagtctgctt tcagatgaag aattactgct agccaaagac ccagaatggg
    23641 tcacaaaaga aatggctgct ttagtccaag cagtaaaatg gatccagcaa gaacaacata
    23701 catctagatt gagaacagaa ttggagcctt ggttacaagc aatcacagaa agttggactt
    23761 tctggcaaga atggcaatta aaaaaagatc caaaagaagc agaagaatgg ttggctaaga
    23821 aaaaaacaca ccgcatagga cgtgatttag taactgccca tttagagcaa attatcaaaa
    23881 gttagatatc ttaaaaaata aaaagcaaga aaaaggagaa caaaatggtc aagagattaa
    23941 ttagtgcaaa ctacagtgat gtcaagaaaa tgacgtcaaa agagttgaag caatccatca
    24001 aagcgagtga aggacgggtg attgtttgtg aaacggttgc taatactctt ccacaatctg
    24061 gagaaattac gaatgcagaa gttgcgtcag catttggtgc agatttgatt cttttaaatg
    24121 gtttcgattg ttatcgtcct gcagtttttg gactaccaaa tgtgacgatt gagaaggttt
    24181 ttgatctaca gcaaacgatt gaaaacccga ttcctaaatt aaaagaacta gtcggtcgtc
    24241 ctgtgggaat taatttagaa cccattgccc ctaaaacaaa tatgatgagt gagaaagtag
    24301 aaatcagcca aggaagagca agctctgttg aaacgataca aaaagcagaa gaactaggag
    24361 tcgattttat ttgctttacc ggtaatccag gcactggagt aaccaattta gaaattacta
    24421 aatccgtaaa aatggcaaaa aagtattttt cgggattgat tattgctggg aaaatgcata
    24481 gcgccggctc agaagagcca gttgtgaata aagaggcgat tatttcttat gcggaagcag
    24541 gcgcagatat tttactagtt ccttcagtag ggtctatccc aggcttttta gagcaagacc
    24601 taattgaagc ggttcggttg gcaaaaaaat atgatttgtt aacgatgtct gcaatcggta
    24661 ctagtcagga aagtgcccga ccagaaacat tgcggcaatt agccattatg aataaaagtt
    24721 gtggtgttga tttacaacat attggagatg ctggatacgg gggattggca ccagcagaaa
    24781 atattttcga aacctcggtt gcaattagag gaatgcgaca taccatcaac tgcgttagcc
    24841 gctcgatcaa tcgctaacaa aattaaataa aaaatggtta aattgattat ttaagtggga
    24901 acatacttga gatatcagct aacttctgtc actttaaaaa agggactgtg acagaagtga
    24961 aaatagacaa aaaatatccg aacaagagac taggatagca gaaaaactat cgttttgcaa
    25021 cgaggaccaa ctggaccgta ggagcaattc tcttattgtt cggatatttc gcttgttcaa
    25081 ggacttatgt ctgtagtgcc cttttttggt aggaaaaatg gtagggaacc atatcatttt
    25141 taatgaaaat caagcatttc aaaagt
//
LOCUS       DACDTH010000036        24907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDTH010000036 DACDTH010000000
VERSION     DACDTH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.036"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(172..624)
                     /locus_tag="IWK54_001608"
     CDS             complement(172..624)
                     /locus_tag="IWK54_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9593730.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(637..1047)
                     /locus_tag="IWK54_001609"
     CDS             complement(637..1047)
                     /locus_tag="IWK54_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9593731.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1322..1534
                     /locus_tag="IWK54_001610"
     CDS             1322..1534
                     /locus_tag="IWK54_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9593732.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1537..1749
                     /locus_tag="IWK54_001611"
     CDS             1537..1749
                     /locus_tag="IWK54_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593733.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(2041..2265)
                     /locus_tag="IWK54_001612"
     CDS             complement(2041..2265)
                     /locus_tag="IWK54_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9593734.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2343..2531
                     /locus_tag="IWK54_001613"
     CDS             2343..2531
                     /locus_tag="IWK54_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593735.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2857..3192
                     /locus_tag="IWK54_001614"
     CDS             2857..3192
                     /locus_tag="IWK54_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593736.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3425..4366
                     /locus_tag="IWK54_001615"
     CDS             3425..4366
                     /locus_tag="IWK54_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP9593737.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4368..5258
                     /locus_tag="IWK54_001616"
     CDS             4368..5258
                     /locus_tag="IWK54_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP9593738.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5301..6101
                     /locus_tag="IWK54_001617"
     CDS             5301..6101
                     /locus_tag="IWK54_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9593739.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6116..6967
                     /locus_tag="IWK54_001618"
     CDS             6116..6967
                     /locus_tag="IWK54_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9593740.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7168..7629
                     /locus_tag="IWK54_001619"
     CDS             7168..7629
                     /locus_tag="IWK54_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9593741.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7655..8107
                     /locus_tag="IWK54_001620"
     CDS             7655..8107
                     /locus_tag="IWK54_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593742.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8213..8407
                     /locus_tag="IWK54_001621"
     CDS             8213..8407
                     /locus_tag="IWK54_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593743.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8438..8731
                     /locus_tag="IWK54_001622"
     CDS             8438..8731
                     /locus_tag="IWK54_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593744.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8761..9201
                     /locus_tag="IWK54_001623"
     CDS             8761..9201
                     /locus_tag="IWK54_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9593745.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            9276..9743
                     /locus_tag="IWK54_001624"
     CDS             9276..9743
                     /locus_tag="IWK54_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP9593746.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10490..10720
                     /locus_tag="IWK54_001625"
     CDS             10490..10720
                     /locus_tag="IWK54_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593747.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            10879..11712
                     /locus_tag="IWK54_001626"
     CDS             10879..11712
                     /locus_tag="IWK54_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAP9593748.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11705..13120
                     /locus_tag="IWK54_001627"
     CDS             11705..13120
                     /locus_tag="IWK54_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9593749.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13132..14661
                     /locus_tag="IWK54_001628"
     CDS             13132..14661
                     /locus_tag="IWK54_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9593750.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14753..15631
                     /locus_tag="IWK54_001629"
     CDS             14753..15631
                     /locus_tag="IWK54_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP9593751.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15633..15950
                     /locus_tag="IWK54_001630"
     CDS             15633..15950
                     /locus_tag="IWK54_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593752.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            15999..16700
                     /locus_tag="IWK54_001631"
     CDS             15999..16700
                     /locus_tag="IWK54_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP9593753.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            16715..17605
                     /locus_tag="IWK54_001632"
     CDS             16715..17605
                     /locus_tag="IWK54_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP9593754.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17628..17843
                     /locus_tag="IWK54_001633"
     CDS             17628..17843
                     /locus_tag="IWK54_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593755.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17855..18196
                     /locus_tag="IWK54_001634"
     CDS             17855..18196
                     /locus_tag="IWK54_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593756.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18196..18567
                     /locus_tag="IWK54_001635"
     CDS             18196..18567
                     /locus_tag="IWK54_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593757.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18560..18955
                     /locus_tag="IWK54_001636"
     CDS             18560..18955
                     /locus_tag="IWK54_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP9593758.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            18957..19334
                     /locus_tag="IWK54_001637"
     CDS             18957..19334
                     /locus_tag="IWK54_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593759.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19335..19943
                     /locus_tag="IWK54_001638"
     CDS             19335..19943
                     /locus_tag="IWK54_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9593760.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19943..20284
                     /locus_tag="IWK54_001639"
     CDS             19943..20284
                     /locus_tag="IWK54_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593761.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20526..23315
                     /locus_tag="IWK54_001640"
     CDS             20526..23315
                     /locus_tag="IWK54_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP9593762.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            23305..24045
                     /locus_tag="IWK54_001641"
     CDS             23305..24045
                     /locus_tag="IWK54_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9593763.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            24042..>24907
                     /locus_tag="IWK54_001642"
     CDS             24042..>24907
                     /locus_tag="IWK54_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593764.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFD"
BASE COUNT         8985 a         3595 c         5070 g         7257 t
ORIGIN      
        1 agcttctttt tttattgtgt tagctttcga cagtttagtg attcctataa ttaataaaat
       61 aagtccaatc aataataaca ttcaaaactc ctcatttctt tgatataatt attgtagagt
      121 ttactcagaa atgaggaaag agtccatgtg aaagcatggg cttttttatt tttaatgttg
      181 taatactaaa tgttcaagat tatgaggaag ccctaaatat tcaataattt ggaactgact
      241 catatcagat aaatcttgtt catctattgt tctagttaac atttcaattg caaacatatt
      301 agcttcagct tctataccat aaatcataca actaaggttg tttgctctaa aagctgctgt
      361 gcaaatacct ttatggagtc taacgtgggc tatttcatgc cataatacaa atcttttcat
      421 agaaggtgat aggttttgat taattaatat cacataacat cttttattgt gtatcgtttg
      481 tcctaatgtt ttattcccta aatcagtttc aattaaatct atatcagaat atttgattat
      541 tttatatgga ttaatcgcat cagatccaaa ctctacaaat aatttttcta tttcttctaa
      601 tatccagtat ttgtttaaat gcataccaaa gcccacctat tgacgatatt ttttaggagt
      661 gaattttttc tttgctttaa ctttggttaa ggcaagtgca agttgaatgg catcgtataa
      721 tttttcctta tcttcatcgt ctaaaggttc accataataa tttgtttcag catctgaatt
      781 caagccttca agtaattttt cagcttgtaa agcaatatct tgtttatctt tttctgttaa
      841 atcataataa tggcgtttat cagtacgtcc taaaagataa tcagttgata cattaaagta
      901 atctgcaact ttttgtattt tatctccaga tggagtactt ttattccatc tagacatgct
      961 tccatttccg aaatttaact ttctttcaag ttctgccaac gtcattgaac gttcttttaa
     1021 taagattttt atacggtcta atagcatagg atatgcattc ctttcaaaat accgacaaaa
     1081 aaagtatttt tctgcaaatt atctatttac aaatagagaa tttgcagtta tactagtttt
     1141 gtaagcaaga tatttacggc atacaaagac taaaagcaat aaactaatag tctccccaga
     1201 catcagtaag ctcgcttttt gtgcgctgta ttttgtatgc ttacataata gcatatttgc
     1261 agtttttgtc aaacttatta gacaaaaata ttagaaaata ttcgataagg gggtatgaag
     1321 catgacttta aaacagcaag tattaattgc tttagtaaag aaaggttgga gtcaaagaga
     1381 gttagctcga cgaatgggta tatcaattac ttatttacga gatatcttca tcgaaaaaag
     1441 aaaacctaag gaacgtttaa aacaaatcga agaacttttg gatatcaagt tagaagtaga
     1501 ttctaatgat cagccatcag aacaggaggc gtaaaaatgg tacaacgttt gagcaaagcg
     1561 gagtttaaag agaaatataa gtattctgaa agtacttatc aaagaagaat gaaggaattt
     1621 aaaaaatcac gttttagtga ggggtataac gcagttactt ccaaagaaat aacaattgat
     1681 gtcgatttat atgaacagtt tagaacatgg cgttctaaaa atcgatttag agttagagct
     1741 gtttgttaat agctaaaaaa tctaaattca ccacttctga aaagaggagt tgctgctctc
     1801 ctttcaagtt tgccaaagtc cacgtttttt cattttggca actcctcttc ttggaggtgg
     1861 tgacggcgag agacgaagga gggatgacat gaaggaagaa tcaaaaatag ggattttgaa
     1921 tgagaacaat tacacaagat taattattga aacagaagaa gggaaaaaag tagcagaagt
     1981 tacattgacc gatgcaactc ctgctgacgg ttatcgtatt cgattaactc ctaaatatga
     2041 ttaacctttt ggtgggtgtg gatcgtttcc gtgactatca cggctttgaa tttttccatc
     2101 tctgccatga ataaatagct cagatccttg atttttagaa atctctctag cgatttttac
     2161 tgcttctgat tttgtattag tatgtgcagt cgctttagaa tttccagcac ctttgacatt
     2221 ccaaccacca ttggaattag gtacaacatg ttgatttttt cccatagttt tcacctccat
     2281 ttctatagaa atattttatc aaatagaaat ggagaaattc aataaacgaa aggagaagtg
     2341 atatggcata taccttgcaa caagaacatc aaattctcgg tttgattaaa caacgcagga
     2401 aacaattaca agatgaccgt gcagcgctta gaaaagccga tgagctatca gataggcaag
     2461 ctgaactaat tgcttctgaa cttgaggatt tgagaatgct agaaataaaa aatagggaga
     2521 ttagattatg aagaagacag acacactttt tataggattc attttggggt tattagtgat
     2581 tgtagcgcac caaagtatta tcgggggaag cttgttcgca gcattgatgg ttttaatcaa
     2641 tctgcttgat tcaaaagaaa ggagcaacta tggcacgaga agaagcgcta aaaatcggta
     2701 aagtgattgc tgataatcgg tggggaaata gccgccctat tattttaagc aagcaacata
     2761 tcgaaaagca aaaagcatgg caacaaataa aaaagtgact ctgccagcca gcaaagagtc
     2821 acatacaaat ttataaaact aggagaatta tatcacatga ataagaaaat tgaaaatctt
     2881 atcgaagaat tgaaacgtga atgccaaaag caaggtgttt ctattatttg tacagcccaa
     2941 aaagaagggg aacttaaaag tctcgtttat ggtgagacaa cagaaatttt actttgtctt
     3001 gctatgcaag aagaacattt agatgaaaat ctcccattgt cagcacatat tatgcgtaga
     3061 attgctgtgg atgcttacga acaagcgaaa aatgaagaag aaaatcaacc ttcaaatcat
     3121 acttttgtta ttaataacaa ggaagattta gctgatgtga tgactcgtat tctcaagggg
     3181 gaatttcaat gatgaatgaa aaaacaattg atctactcat tattttattt ttcacgttag
     3241 tgattgtagc gaaaagttat gtaatctttg gcttgttatt cgcaattacc atgcttgtgc
     3301 tttgcgtgtt agcaagcaga aaagaggtta aacatgaacg agttagcaaa tcttgacaac
     3361 tatttaactg atcctgatta tgctaaacca ccatatgaag caccaattga tgaggaggat
     3421 gaagatgagt aaatctacct tagaaatgag ccatcaagaa tggcttgaag accgtaagaa
     3481 aggcatcgga ggttcggatg ttggaacaat tttaggattg aataaatgga aatctcctta
     3541 tcaactatgg ctagaaaaaa caggacaagt agtacttgaa gaatcaggaa gtgagcccgc
     3601 ttattggggt aatgttttag aagaagtggt tgctaaagag tttcaggaac gtacaggcaa
     3661 aaaagtacgc agaagaaacc aagtatttga acatccatta catccatttt taagagcaaa
     3721 tattgatcgg gaagtagtgg gagaaaatgc cattctggaa tgcaaaacag ccaatcaatt
     3781 tctcggtaaa gaatgggaag gcgaagaggt accgcttagc tatctctgtc aagttcaaca
     3841 ttacatgaac gttctaaaca aagactattg ttacatcgct gtcttaatcg gtggtcaaaa
     3901 atttatctgg aagcggattg aacgagatca agagctgatc gatacaatca ctgaacaatt
     3961 agtagagttt tgggaaacaa acgttcttgg aggtatcgag ccaattattg atggtagtca
     4021 agcgacagct gatttcttga aagagaagta tgcagatgtt gaagacgttc aaacgacgtt
     4081 accaattcat ttcgatgaac tagttgaaca gaaaaacgaa ctcaaacgga ctaagaaaga
     4141 aattgaatca gctatccgtc aagtagacaa cgagattatc agcgagctag gtaaacgtga
     4201 agccagtatc ggtatcactc aaaaaaacat catcagttgg aaatttgtcc gtacgagacg
     4261 tatgaactcg aagaaactag cagagaaata tccagatgtc gcaaatgatg aagagattta
     4321 taacgttact gaatcaagaa ggctaaccga aagggagatc aaataatatg gcaacaaatg
     4381 aatcgttaaa aaatcaattg gcagcaaagc cacagaaaca agttgcacca ggtcaattag
     4441 gacttaaagc tttgatgaat acaccaacaa tgagaaagaa gttcgaagag gtgcttcatg
     4501 acaatgctaa tgcttttatg tcgaatgtta tgactcttgt atctaatgac agttatcttg
     4561 cagatagtga accgatgtct atcatgagtg gtgcgttaac tgctgcaaca ttaaatcttg
     4621 ggctagataa gaatttaggt tatgcatatt tagttccatt caatagtaaa aacaagcaaa
     4681 caggaaaatg ggaaaagaaa gctcaattca tgcttggcta taaaggatat atccaattag
     4741 cccaacgatc aggtaaatac aaagcattaa atgtgatcga agtttacgaa ggagaactaa
     4801 aaagctggaa ccgactgaca gaagagtttg agtttgatcc aaatggtaga acgtctgatg
     4861 aagtcattgg atatgttggc tatttcgagt tactgaatgg attcaaaaaa actgtctatt
     4921 ggaccaaaca agaaatcgaa gctcatcgga ttgctaacaa taaagatcgc gataagacaa
     4981 agttaagtgg tgtgtgggca tctgattaca atgcaatggc acgaaaaact gttttgagaa
     5041 atcttctttc taaatgggga atcttgtcca tcgaaatgca agaagccacc acatcggatg
     5101 agagagtcca aagggttcaa gaagacggca gcattattgc tgaaacagaa gttgaagaag
     5161 atattcctga aagaaaagaa gcagaggtta tatctgaaga aaatgaagat gtacaaactg
     5221 gattatttga tgcatctaat ccgccgttaa acaaataatg agggagcttt ctcccttgct
     5281 tttctagagg agaaatacga atgaacacag gatatgtcaa attacatcga aaagtgatga
     5341 attcattcgt ttggaccaat ccttatatgt ataaattatg gaacttgtgt ttgatgaaag
     5401 cgagtcatga gaatcgcaag atacttttta atggaaaaga gatacaagtg aacagcggag
     5461 aattcgtcac agggcgcgat gctatcacat ctgagatgaa cgaaggcgtg aaacgtgaac
     5521 atcaagtgaa cagcggttct gtatggagat ggctaaaaca atttgaaaaa aacggaatgt
     5581 tgaacatcaa atcaactacg aaatacagcg ttatatcaat aaaaaactgg tctttgtacc
     5641 aaagcagtga acaacaaatg aacatcaaac gcacaacaag tgaacaacaa gtgcacacaa
     5701 acaagaatga aaagaattat aagaatgaaa agaataataa aacatcatcg tcgcaaccac
     5761 gcaaaaagcg tgtttacgac accgactcag tttactacat tctcgcggag gagttattca
     5821 aacagatttg tcagaatcag gaaatcaaaa agccgaatct gcaaagttgg gcagatgaca
     5881 ttcggaaaat gatcgagatc gacaagcgga ccgaaaacca ggtacgggga atgattgaat
     5941 ggagtcagca caatgtgttt tgggcatcga atattttatc tgctaaaaaa ttgcgagaaa
     6001 aatacgacac aatggcagcg caagcaaatc gtgattataa aacaaaacaa actaaaacgc
     6061 ttgaatacga gaaatttagc acagatgagt tgcctatttg agaggaggcg taggcatgga
     6121 aactgttggg gaaatcatgg aaaagctgat acagaaagtg cttgtccaac gtggcgaatg
     6181 tcctgaatgc ggacagcctt tgtatggatg gcgtacgaag aaccctgatg gctcagaacg
     6241 ttgtaagcca acctgtatgc aatgtgggta taaagcattg cgagttcagg aagacttaca
     6301 aacagaacga atttacaacg agagcctgaa agcaagagca atcaattttt tcaaaggtgg
     6361 ttctgttgtg cctaatcaag cgttgtttga ttgcacattg cagaattatc aaattgtcga
     6421 tcaagaaaca agacaagcgg ttgaagtaac caaacgcttt gttaattcag tcttgttagg
     6481 aaatccaagt caccttgttt taactggaaa acaaggaaca ggtaaaagtc acctagcgat
     6541 ggcagcggct tgggaagtct tgaagcgatc aaactacgat aaaaaaatct tatttatcgg
     6601 gttacaggaa atgctggatc aaatcaaatt ttcgtacaac aatcctgaac tcagaaaaac
     6661 gattgaggga tcgttgattg cagatatcaa aacagcggat ttagtcatca ttgatgatat
     6721 cggttcagaa ctaggaaaag atgcatcaga tagtcgagcg tttggcataa acacgctaaa
     6781 ttcgttcttg gatgcacgac agaacctagc aacgattatc acaacgaact tgcttggtga
     6841 agaactgaaa aaagcttacg gtacgagaac aatatcaaga atgtttgtca actctgatgg
     6901 atttacgatg gtattttctc aaacagcaga caagcgcata aaaccagtca aaggagcgat
     6961 cgcatgaatt tgactaaaca agaaaaaaga atctgtcgtc agataatgtt gcaaattata
     7021 ggtgatcgtg acatggacga attatctcaa caagagagtc gaatggttcg gaagataagt
     7081 aagctaatta tttatcggga tacagaaaga tttctcactg ttttaggtaa cgtctacgag
     7141 aatccagagt tattggaggt agagtaattg ccaaaaatat tagatgcatg ttgcggtagt
     7201 agattgttct ggtttgataa gaacaattct gacgtaacat ttatggattg tcgtcagcag
     7261 tatgaagaat tatcaacagg tcatgtgatc aatgttgatc cagatgtggt cgcagacttt
     7321 cgcgatatgc cttttgaaga tgatgtgttc gatatggttg tatttgaccc gccacactta
     7381 agacatgtcg gcgaaaactc atggcttgcc aaaaaatatg gaaaattaga cgaactttgg
     7441 ccagaagaca tcaggcaagg ttttgcagag tgtatgcgag ttttacgacc ttcaggaaca
     7501 ttaattttta agtggaacga ggaacagata ccactttctg atgtgttaga ggctattgga
     7561 gaacagccat tgtttggtaa taaacgaagc aaaacacatt ggcttgtgtt tatgaaaaaa
     7621 ttcagctaac ggaggaaaca aaggaggaaa agtaatgaag attgtattaa ataaatgttt
     7681 tggtggcttt gggttgagcc atgaagcaaa aatggaaatt tttaaaagaa aaaatattga
     7741 agtattccca tacatcaata actttagcta tgactcagat gatgaataca cgagatacac
     7801 aggccaaaag ctaggttcga tggattttat ctactatttc aaaaaagacc cgaaaatcga
     7861 caaagttaca ggaatttata gcgaaattga ccgactgtac ggaattgctg atgattccag
     7921 tttttcaagt gattcaaaca gaggtgataa ggacctcgtt gctgttgtcg aaaaactagg
     7981 aagtgaggcc agtggaccat atgcaagtct taaagtcgtt gatatacccg atggggctga
     8041 gtgggaaata agtgattatg acggcgttga aactgcacat tatggttttc aaacaggtag
     8101 tatttagtcc actaatgaca actaaagagg aggcagaagg atgagtgaag atgaatactt
     8161 tgaacagtag tctattaacg acagagattt ttaactggaa aggggtgtag tcatggacgc
     8221 atcggaagta gtaaataaaa ttaatagtct caaagaggtt ttcggagacg tcgaagtagt
     8281 gataaacgca caaggctatg acaacgaatt ttttaagcag attaccgaca ttagcattca
     8341 gcaaggaatg gaagacgaag acggaaactt tatcgatgaa gtagccattt tagtgacttg
     8401 cgagtaagta attgacagct aaagaggagg aagcggaatg aaactaaaag acggatttta
     8461 ctccagcggc cacggcatcg gcggtttaat gctagatatg ccgacaaaga atcctaaaac
     8521 acgtaagaaa ccaaaattca aagtcggtga catggttcgc tgcgaagcag aaggattcat
     8581 ctatccattt cgtggatatg tagaacacgt ctataatcac tcagcaatca ttcgcattga
     8641 aaacacgatg gaatgtgata agtggttagc gaaaagcaaa gagaatttag cagtggtgag
     8701 attggtggac atggaagtta taaaatctta attttgcttt tttacaacta tcctacaaca
     8761 atgagcgtta ttccacaact ggaaggagaa gaggcaattg tgaacatttt agagaatata
     8821 gatattaagc aaacaaggaa aaatgctaga cgattactaa aaagatacag aaatttagaa
     8881 cgtttggtcg gaccagtgaa aatagacttt tccgtgatga ctgttaccaa aaatttgaaa
     8941 ttcacaattg acagtcaaaa cgaagaaatc attgaagcga taagtactcg agattcggtt
     9001 atcgaagcat taacacggct aagtagaatc catttccagg tactttatta tagttattgt
     9061 tttcctaata agatgtcaat gtatcaaata ggagaaaaat taggatattc cgatagaact
     9121 atcgagagaa tgaaggcagt agctttggtt gaatttgctg aggcgtataa atcaggagaa
     9181 ctcatttcac gtacaaaata aaaaaagtcg gattcctccg actatgaata atatttccga
     9241 cataagtatt atatcataat tgggggaatc agaggatggt acttttcgat gtaaagaaat
     9301 atgagacacc aagcgcgaaa gatgttgata tggaacgcac aaaacataat gtcgctgtgt
     9361 ttctttcagc atatctatca gctagatgta gagtagggca acatcgagaa ccaaaagtga
     9421 cagcatctta ttccttggtt ccaccttcta cagctgatca tatatttgaa gcagaaagaa
     9481 tgctgatcga taaagaagaa gcacaggaag aatttgagta tctgcataaa ttgtttgttc
     9541 gaggctattc agcgatacag catccgcata agcctgatgt gactgaaagg cgcaagaaga
     9601 tattctatga tcgttatatc aatggtctgc ccatttatgt aactgctcaa aggaataata
     9661 ctagcgaaga atcggttaaa gtagaatcaa acagaattat catccaattt gcttcatcgt
     9721 tagaactggt tgctttcaag tagccagttt ttacactttt tatacccttt tattaccaat
     9781 ttggtttcca ttttatacct tttttatacc aatcacttac ctattcaacg ttgtattatg
     9841 atagtgtcga aaaattagga aacaggatcg acaaaataaa atgtaaggga ggaaatctcc
     9901 ctcatcgttt aattaagctt cgataggcag caacggaaat attaagaata aggatgtgaa
     9961 tttcaactcc ttctaaattg ttcttattat ctatcatccg ttgctgtcta ttgttttttt
    10021 aattattcac acgataacta aaggtaagtg aagagaaatg attccattaa taatttcaat
    10081 ttttgcgctt tgtcttaatg tatatatgat tggatttaaa aatgggcaaa ataaaaaata
    10141 gtagcagcca agaataattt tatagtgtca ctgtggcgga aagggtagac gctaagcatg
    10201 tgtgctaggt caatgcttcg gcaaccatgc aaggttcgat tccttgccag tgactttggc
    10261 aaccgaggca tcggcggttt aaaaatatag gggtgcgcaa tttcgtacgc gttttgtgca
    10321 tcgtgtaggt tgctattaca taactggtta ggttagattg agatttggga ttcggtacaa
    10381 atgaatcgtc aaatgtctca agcacaggat cggaaacgtc cctgcctgtg cattacatat
    10441 tagatcactc tttgagtggt ctttttcttt acataaagga ggctacataa tgagaaacta
    10501 ctggtatgta tcgctaacta atgaatatcc tcgaaccatt gatgattgtt cagtgcgtgt
    10561 tgtgcgttct gtacaaatca aagggaagta ctctattgtc gaaatgggga gagaagcaga
    10621 accatgtgag attgatgcgt gcaagctcgt ttattgcggt catggtttct atgatgaacc
    10681 aaacattcaa aataatatta acaagaattt gagggattag aatgcaagaa atggcttaaa
    10741 aatctccaat caggacaaat caagttagac aaagtttccg atttaaagat attgattgaa
    10801 gctgatttaa tgctaaaaga tattgaaaat tagaaaacaa aactcaacct aaacagaatg
    10861 tgaggtggtg tgtattgaat ggcaagacaa cgtgatccaa gacgtgatga agccaaaaga
    10921 atttggttag aatccaacgg agaaaagcag ttaaaggaaa ttgcatctga attaaatgtt
    10981 tcagattctc aggttagaaa atggaaatcg caagacaaat ggagcgctga attgattagt
    11041 aacgttacca atggcaaaag taacgttact aatcaaggtg gcgctcctat tggtaatcaa
    11101 aatgctaaag gtaacaaagg aaatagccga gcttctccgc cagtgggtaa taaaaacgct
    11161 ttgaaaacag gtgagtatga aacaatattt tttgacacgt taaccgataa tgagaaggac
    11221 atctattcta gtttgaatga tgatccttct tttgctttgt ccgaagaaat acgacttctt
    11281 aaaataagac aacttcgaat gatgaaaaga atccaacaag ctgaagctgg actaaatgat
    11341 gaagaagtcg aacgattgca gcagctaaga aagattaaaa ctccgattga aaaaaatggt
    11401 aaaaagctag aaatcaagcg tgaggttatg caagatgtgc agattagcag aaaaaaacat
    11461 cgcaaaattg atgatattct ttcaattgaa gattcattga ctcggattag caaccagtta
    11521 gctaaagcca tcaagcaaat gaatgaactt tatatgaatg aatacagaac tgatttaatt
    11581 aaagctcaga ctgataagat ccaagctgag acaaatgaaa ttggcggaaa taattcaggt
    11641 gaagaaatag aagaatggaa acaggcagtt ttaaatgccg caaacaaacg ggcggtgaaa
    11701 gaaaatgaat aatgaattta ttccttttgc tgatattggt tctgccattg attattacta
    11761 tgataaacca gtagcttttt gccaagatat tttgcatttg aatcctgatg aatggcaaga
    11821 aaatgtttta aatgatttag ctgaattttc aaaggtttct gttcgttctg gtcaaggagt
    11881 tggaaaaaca gcattagaag caggagcaat actttggttc ttaacgtgtc gaccctacgc
    11941 taaagtaata gcaacagctc cgacaatgaa gcaactttac gatgtacttt gggcagaggt
    12001 agctaaatgg ttaaatgata gcttgatcaa aaacttactg aagtggacaa agaccaaaat
    12061 ttatatggtt ggtgattcag agcgttggtt tgctacggct agaacagcga ctaaaccaga
    12121 aagtatgcaa ggttttcacg aggaccatat gttgattgtg gtagatgaag cttctggtgt
    12181 gtctgatcca attatggaag ctattcttgg tacgctatca ggttttgata ataagctgtt
    12241 gatgtgtgga aaccccaata atattgaagg tgtcttttat gattcccata attcagaccg
    12301 tgataaatac agagttcata aagtatcaag ctatgatagt aaacgtacaa acaaagacaa
    12361 tatagaaatg attcttaaaa aatatggaaa agaaagtgat gttgctcgtg tccgtatttt
    12421 tggagaattt ccgaaaggtg cgttggattc atttatcagt cttgaaacag ttgaattggc
    12481 tacagaaaaa caaattagtg attctttagt caataaaaca aaggttgctc atattggtgt
    12541 cgacgtagct cgatatggtg atgattctac gattctcttt cctagaattg ctaccagggc
    12601 attggagtat gagaagtatt caaaacgtag caccatggaa acaacaggat atgtcatcaa
    12661 catggccaag aatctaatga gtcaatatcc gagtattgat aaagtgatga ttaaagtcga
    12721 tgacactggt gtcggaggtg gtgtaaccga ccgcctagaa gaacttatag aagacaaaca
    12781 ttatcctttt gaggtgtttg gagtgaataa cggttcaaca tcagaagacg atttttacga
    12841 taatttaggt actcaactat ggggaaacat caaggaaatg ttagaagaaa atatgacagc
    12901 aaatcttaac ggagaacagc ctgttattga attgccttct gatagttcgt taatcaaaga
    12961 attaagtact cgcaaattca aaatgacaag tagaagtcgt atacgtttag aaagtaaaga
    13021 tgatatgaaa aagcgaaata ttggtagtcc cgatattgct gacgcactgg ctttagcgtt
    13081 ttatgagcca ccaagtcact atcaatttat tcaattttag gaggtgagct tttgattagt
    13141 acagtattat ctttagagag atataagaag ctacgtgtta aatatgcaac acaaattgaa
    13201 gatggaatgt ttgatccgaa tggttttata gaagatatga aaccattttt tgctgatcgc
    13261 gaaagaaaat atctagctta tactagcgaa aagaatgaaa tagataatag accaaagcct
    13321 aacactgata ttgtaaaagt taataataaa cttcatgctg gtatgtattc aattgtcgta
    13381 gaccaagcag tcaatcattt tactggtata cctatcaaat gggattacga tgtatcggaa
    13441 caaaaaagaa cactcattca aagattaaaa gataaattct taaaaaatga tatagaactt
    13501 ccgacagttc cagaagcttt taacaaatta acaagcaatc ttgattccat gagattcgca
    13561 atgcttgatt ctgaaactgc aacttttcaa ggagcttgtg gagtagcttt ccgattgcta
    13621 gagcctgtgg aagaagatga tggttggaag ttaagagcaa gtaatattga accttggaga
    13681 gcagaaagat atggaaatgc cggaatctat atcaaagaaa agtatgactc ttaccagaaa
    13741 aaattttttc aagaaatgaa agttatgaca agaaataaaa ttctgacata tgcttgctat
    13801 ggtgatttga attttgttac aagcggaacg ttcaaaaaaa ttgatgagac cgataaccct
    13861 ttggggacga ttaccttggc agaatttaaa aataatacga atcgttattg tgattttgaa
    13921 gtagctgagg aaattggaga tgcaattgat cgtgctttgt ctgatcaaca aaacgaaatc
    13981 gaacaattta agcttgcgta catgcttgtt actggcacaa caatgagcaa aggcactgct
    14041 aaagaaatga tgaatcagct agggattatc aacttaaaag cccctactgc aaaagctgaa
    14101 tatgtcacaa aaaatttagc aaaagatttc aatgaatatc atatggatct attgaaaaaa
    14161 cagttttaca ccatttgtaa agcaatcgat tttaacgatg aggtgtttaa atcaaacagt
    14221 tctggagaag ctcgcaagtg gcaaatcatt agcctagaag cgaaaacaaa tactaaagag
    14281 caatatttta gagaaggatt gaaagaatgc gcagaaacaa ttgctgcttt tcttaagttt
    14341 catgacaaag tagaaattga gcctgaaaaa attattttta ctttctctag atctttgcca
    14401 actgatttaa gttatttagc tgaagcttta cctaaacttg caccatacgt atccaaacga
    14461 actattcaga gccaaattcc atttgtaaca gatgttgatt atgaaaatga aatgatggaa
    14521 ttggaaagtg gaagtgctta tccagatagt gaatacaatt ttggcggagg tggcaatagt
    14581 gacgatagaa acgaagtatt ggaccaaacg tcgagaactg gaggatcaag caaggctcaa
    14641 acaagaaaat cagacactta aaaaattaac tagtgtattt cctgaagcac tgaaagagat
    14701 acaagcaaaa ctattatcac aagctgactt acacaatatc acttatccag aaatgatgga
    14761 gttttacagt acaagtaatc agaaaaaata tcgtgaatat gtggaaaaaa attataagtc
    14821 attaaaaatg tatgatgcaa aatacaaaga gtttatcgat gaattttttc caccatttga
    14881 ctatgcaaaa gtcaatcgct tattacaaat acgatcagat gtatttaaaa ttcttgcaga
    14941 atatgcgatg gatgcagatg tgaatcaata tttttctgat cgcttagagg aaattcttca
    15001 aagaacatat tcttctaatg ctaatgtttt tgttcaactt ttaaacgttg atataccaaa
    15061 ttatttacca gaaaatgaaa ttaaacatta tttgaactat ccatggtcag gaaagacatt
    15121 ttcaagaaga ctttggggaa atatttcatc gcttgaacag aaactctcta atgctattgt
    15181 aaaaagtgtt gctagtggag aaggcgttat acacgcatta aacaccatga gattagactc
    15241 agaaatttgt gacatgttta agttagaaga atcaaagtat aacaaagcga tagaaaatct
    15301 cgttcgaacg gagtatgcaa aatttgcaca agatggtatt gaaaaatcat atttagaaac
    15361 aggtattgag gaatacaacg tattgactgc aaaagatgag agagtttgcc ggatttgtgg
    15421 aggaaaggca agtaagaatc cctataaact gaaagatgct gtcataggtg aaaatcgagc
    15481 acctttccat agtcgttgta gatgtacgga tgttcctaat ttaccaaaat taggaaagga
    15541 tattgatgaa gaatatgacc gtttatttgg cgatttatta gatgagtttg cacatgattc
    15601 ttttggaatt aatttgaaac ggaggaagta gaatgaaaga tttttttgaa gcagtactaa
    15661 caattaatgt aaatgctgat attgcagaag cctacaaaac agctattgag tctgagaacc
    15721 atcctaatgg cttgagagac cattggaatg gcaattatgc ctacgtggtt attggcgatc
    15781 aaactgttaa ttatcaagat aatactccag ttgataagaa taccgttaat ttaacgattc
    15841 aattattatc tcattcatta ccaaatttaa aagaaacagt tgattggtat gaaaagatgg
    15901 gatgtattgt tgttagaact gactacaaag aaggaaagtc tagtaattag gcttttttat
    15961 tttgtccgaa atgacgttaa actagcgcaa tactgggctt ggttgaatgg tggggcgcaa
    16021 ctattaaaac tcaaagcaat gcggggcgtg aaaacgaatc gtggggcgaa aggagaatga
    16081 gcatgaaaca caaatcatta atgccaatga atttgcaata ctttgccgaa ggtgacgatc
    16141 aaaagttttc ttttgatgac ttcaaatctt ttgtggaatc caatgaagaa gctcaaaaat
    16201 ttattcagtc acaatctcaa agtgttgccg acaaacaatt ggaagcttgg aaacaaaata
    16261 acttagataa aattaaacag gataccatca aggaatatga ggaatctaag aaaaacaagt
    16321 cacctgagca aattcaactg gaaaaactac aagctgaatt tgaagcagaa aaagcgttgc
    16381 gtgtgacaag tgataataaa gcatttgttg cagaacaaat tgctggatta gaactagatg
    16441 gagagttaaa agagtctatt tctcaattta tgctaaataa tcttgttagt tcggatacag
    16501 atttcactaa gaatgctgtt gaaggtttta caagtgtttt gaatgcaatt aatgagaaac
    16561 atgcagatgc attaaaagaa ctacaaatga agtctgcatt tggtggaact caacaatcga
    16621 ataaccaggt tcagcagaac aatgaaacat taacaaatcc agaagaacaa ttaggacaaa
    16681 ttcttcaaca atttaactag gagagtgaaa aattatgaaa aaaacatctt taaataattt
    16741 agagtatttg gatatttcac cagcgattaa tgctatgcaa gtaccaaata cacctttttt
    16801 aagctattta cttggtgctg gaaaaacaga gccagcaaac tcgacagaaa ttaaatggcg
    16861 tgaatatgat atcaacaacg atgattcttc tgaaaaactt gagggcggag aatatccaga
    16921 tgctgaatca ggtcgaactt ggtttaacaa ctatactgaa atttttagaa aatcaacctc
    16981 tgtatctggt acattagatg ctattaatgt gaatggagtc ggaaatgaat taactaatca
    17041 agtagcacta cgtggtatgg aaatgaaaat tgatttgaac cgaaaattga ttactggtgt
    17101 aaaagctgat gaaaatggtt ctaaaggtcg tcgaatgaat gggattttga acttgatcaa
    17161 ttcagcaaat aaggcagaaa cagccactgc gggtgcagta acaagaaaag atatcgatac
    17221 tttatttaaa ttgatgtatg aaaaaggtta tatgggagaa aaactatgct tgatttctcc
    17281 agatatgcag gagttaatga ctgatgagtt agatggaaaa tcaacaaaaa ttgttcagtt
    17341 cggggaaaga gtaacttttg gattgcaaat tggaaatatc gtgtctaatt acggtacagg
    17401 tattgctcta ctagaaccat cattgccaaa aggaacaatt gccgcaatcg atactaatta
    17461 tgtgaaacta cgtccattac gtgaatggag agcagaagaa cttgcaaaaa caacggattc
    17521 tagacgtatc ggtcttgtag gtgaatactc tcttgaatac aatgcttcaa actctggggc
    17581 aattttaaat ttaaagtctg aataaaaggg agttagtact cccttttttg ataggaggaa
    17641 ttacagtggt aaaaaaagat gaaactaaaa aagatgaagt cgtgaaatat agagtaggta
    17701 aaactaaaaa ttttgttggg tttgttcatc ctaaaactcg tagatttatc acagcagatt
    17761 caaataacga atttatcatt tctatagatg ataaagaagc aattgcaatt ttggaagatg
    17821 caattgatgt taatgaaatt taggaagtga tctgatggat gaatcgctaa aaacggaaat
    17881 cattgagtct acaaaagaag attttccaga tttgagtgaa gaacgcataa ctaatttatt
    17941 agaaataatt ttgctagaaa ttgaatcata caacacttgt aaaaatgata tttcatggga
    18001 aaagttaaaa agcgtgatta gtgaagtgtt gtataaaata ataaaaaatg aatcagaaaa
    18061 aacagtatct tcagttagac gtggcgatac aacgattagt tatgcttcaa caacaaatga
    18121 tgttagtgag ttacttctag gctatggtga tttgatacga agagttattg gttgtggagg
    18181 attggagttt ttttaatgaa tgaagcagat attttagaaa ctacttatga agatagttgt
    18241 attattgaaa gactgacgga cattgaagat tctaatacaa atattactat tcaagattac
    18301 aaaaaagtat atgataatcc tatttcttgc gctctttcac aaggtcaaat cgatggacta
    18361 gcagtcatag aagatggaga gatggtaaat gtttcaactg acacatataa attatttgtt
    18421 catcctaaga ttaaactaaa aaaaggagat cgaataacaa taactcaaaa ggccagtggc
    18481 ttaatttttt ctctatttgc tactaagcct ttttactatc ctagtcattg tgaagtgaat
    18541 ttgataggaa gtgagaaaaa tgggtaatct caaatatgaa tcaaatgcag aaaagatcat
    18601 tgagaatttt aaaaatatga ctgtaattgc tcaaaaagaa ggaatatcct tcgttaacga
    18661 ttcaatgaat aaagtcgtta gtttagctaa accattaact cctgtaaaat caggtaattt
    18721 aaggcgagga tatcgtgtag taaaagctag aaaactatca agcggtcgaa ttgttggaga
    18781 agttataaac aatgaacatt attttaaata tgtagaagaa ggtcatagga ctaaaaatgg
    18841 tggctttgtc aaagggaaat tcatgttaac tcgtgcaacg aatcttgcaa atatgtctta
    18901 tattcctcga agatttaaac aaatggcaat aaaaatcgtt aagaaaggaa agtaacatga
    18961 aagatgaaat cattgctgca atcagtagca aattaaaaga aatctatcct gatgggacaa
    19021 tatatcttga ttcagttatg cagtcaacta aagattttta tttcgtttta tcagtaatgg
    19081 aatctggaac tgaaaatgta ggaattgata ttcaaaatgt ttctttctta attgatattg
    19141 cattgattga taataaccct aatagaaatt taattaatga attagtctca cgttgtggga
    19201 ctttttttaa tacgattaca atcgatgagc aaatattatt tccaaaagcc tatttacctg
    19261 atgaagcaga tggtgttcaa catattcgtt ttacattaga atttccacaa tatattgaat
    19321 ggagtgaaag ataaatggga gaaaaaagaa gtaaaaccgg aattatttct gtcgaaaaac
    19381 ctacttggtt tcctttggaa gatgagacag ggaattttcc ggtatatggt actgcgttgc
    19441 caatgggaac agcagtaagt atcaaaccaa cagctaatta tgaaacaaca caagattatg
    19501 gggattcagt ggttcaagat caatttacag cttttggtgg tgcagaagtt gaactggaag
    19561 caaatggata tactcataaa gttttatcta caattactgg agggaaaatt gttaaaggtg
    19621 gtgccttgcg ttctggagaa gatattgcgc aagatggtgc gtttgcatat agacgtaaaa
    19681 aatcgaatgg gaaataccgt tatacggtct tttataaagg acagtttgct ctagattcag
    19741 atgaaacatc aacgattgaa ggaagtaaag taagttttac tcatccaact tggaagggtt
    19801 catttgtaga tgtaccagga cttggatata tgtattcagt tgatgaagat gatgaaggcg
    19861 tggataaagc aatgatcgga aattggttta caaaagtagc aattccaatt gaagaagcag
    19921 aactttcagg aggtacagaa taatgtcaaa atatcaaaca acgattaaat taacgaaaaa
    19981 agacgaagaa ggtaaatatg agcaagtaca atttaaatct gctgaatttt taccaggaac
    20041 agttgtagaa gatgctgcag gagttatgga agaaatgcaa acagcgactg ataagcaatc
    20101 agttaaaaaa gctttaagtc gtgcttattc atttattgca gatactttat ttgagggaca
    20161 atttactggt gaagattatt gcaaaggaat tgatgctcgt gagattgcct cattgacagg
    20221 aaaattattg aagtctgtta ctgcaggttt tgatgaaact tatacagaga cgaaaaaaaa
    20281 gtaagtgagg cgctcaagtc accttcattt aagtattcga ttacttaccg agaattagat
    20341 ataaagacgc aattactcga agcaggttgg acgttaccag aaattgaacg tactgacttg
    20401 gatgagttaa tgcgtctttt tgcttttaga gatgcggtaa aagagcatga agatgttgag
    20461 tactatgata atttcactca attttaggag gtgatacttt gaataacgat gacctaattc
    20521 tgaagatgat actagatgaa tctggtttta ctgccggtat gaataatgct gtcaaaaagt
    20581 tgaactcttt tgatgaaaca atcgaaagaa aaagtagaaa tagtggcagt tcattaggta
    20641 gtatttggaa aatatttgct ggaagttttc tagctagcgg agtaactaga atcgtaggag
    20701 ctggctttga tctaatcaag ggttccataa gtggagcggt tgatcgagta gatacgatga
    20761 ataacgctct acgaaatttc caaaatatgg gatttagtaa ttcagaaatt atgaagaata
    20821 tcggaaagaa tgggctttta tctcaaggta ttcaaggact tcctactgct ttgaatgatg
    20881 cgataagtca tgttcaactt cttgcttctt ctacaggtga tttaactcgt tcaactcaaa
    20941 tatttaaagc tttaaatgat ggaattcttg gtttcggtgg ttcaactgat caagttaatg
    21001 aagctgttat tcaattatct caaagtttct caaacggaaa agtagacgca caaacttgga
    21061 actcaatgat caacgctcaa cttggaccta ctctttctgc tattgctaaa aagatgggaa
    21121 ttacaatggg agatctgaaa gaaggtttat ctcaaggtaa gatttctgtt gaagaattcc
    21181 aaaatcaatt aatagaaatg gataccaaag gtggcggagg acttaaatca ttaagtcaaa
    21241 tcgctaaaga ttctactaag ggaattaaaa cctctataca aaatgcaaaa acagctgtta
    21301 cacgtggtgt tggtgaggta atagaaggat taaataaagc attagtagat tcagatttag
    21361 gtggatttaa gggaattatt gataaagtgg gcagttcaat ggaatcgttc ttaaaagtaa
    21421 ttgctgcaaa tattcctaca gcagtatcat ttttaagtaa tttatttaac gaaattcaaa
    21481 aatttggttc tgctttgaaa ttcatgatgc cctttcttgt tcctgcagct accgcttttg
    21541 gagcgttcat gtttcaactt aaaggtatac cagcaataat aaaaagcttc aataatttta
    21601 agaatgccat aattggcgtt ggaaattcat taaagattat gggggcaata gctgctgcta
    21661 atccatttgt tttaattatt ggtgcagtcg ttggtgcaat agctgttttc ggttacttta
    21721 tggcaaccaa tgaagagctt agaaacaagg ttatatctat ttggaatgat gtgaaagatt
    21781 ccgtaattgg tgtattaaaa aatataaagg actggggaat tgatacttgg aattctgcta
    21841 aagaaatggc atcaaatgca gttgagggtg tcaaagatgc ttggtcagga acaaaggaat
    21901 ggttttctaa cacctggaaa ggtattaagg atggggccac aggtttattt gataaaacag
    21961 tagagacttc aagaaatgca gtcgatagtg taaaaaatgc atggtctaat atgaagaaat
    22021 ggttttctga tacttggcaa agtataaaag attcagccag agaaaaatgg aatggaatca
    22081 aaggttctat tatgtcagtt gctggtccat tgattacagg tataaagaat gcatttttac
    22141 atgttacttt ttatctagag accttatgga ataatttagt tgagattggt aaaaacgtct
    22201 ttgagatttt aaaaaatgtt atacttgcgc cggttttgtt tattacctcc atgatttctg
    22261 gtggatggga agaaacaaaa aataatatga ttgctgtttg gaataatatt aaagaaagtg
    22321 ccataaatat ttgggaatct ataaagaata tatttgtaag ttattttaca aatatttatt
    22381 ttgctgcact caatatttgg acagggttca agcttacatt gataaatatt tggaatgaag
    22441 tggtaaatca agctaaatcg atttggttaa acataaagta tttttttatt aatctttgga
    22501 ttgatataaa atattttgca attcagaaat ggattgaatt gaagttttct attattcaaa
    22561 cttggatcga tttaaaatat aatgccatta ctacttggaa taatattaaa cagtttttca
    22621 aagatacttg gaaaaatatt aaagatacgg catacaatac gtggatttct ataaaaaata
    22681 ccatgattaa tacatggaat aacattaagg actctttctg gaatattgtt actggaattg
    22741 ttaattctgc tgaaaatgca tggacaaatc taaaaaatgg tgtttcaaat gctattaatc
    22801 gggtaaaaga aatctttaat tcgttaaggg aagtcaattt atttgaaatt ggcaagaaca
    22861 tcattgatgg acttataaat ggtgtaaaag aaaagtggaa tgctttgaaa gatactatca
    22921 aaggtattgc aggtaatatc aaaaattgga ttaaaggagc tttagatatt cattctccat
    22981 caagatggat gcgtgatatg gttggtaaaa atattgttca aggtattatc gttggtattg
    23041 ataaagagca aagtaagtta gatcaaacca tgacaaattt agtaaaaaca ccgtctgttc
    23101 aacctgtagt aaatggaata agtcacctac caattacaca aacaaagcaa aatatatctg
    23161 ctagtgaaac aaaagaaatt catttacatt taaatgttta tggagattta cctgattcaa
    23221 tggttcggaa gattaccaca aaaattaaaa cagaattgac aagacaaatg aaacgagatg
    23281 ctggtgcagt aggaggaaca ttatatgcaa cttaagagag gacaattttt tattaatcaa
    23341 cattattctt ctgaatttaa tgtgtatatt caaaacaggc ctgcttctgt ttcagctagt
    23401 cgagtaattg aattgagaga gcgagaagga aatgattcta tcattattga taaaggctat
    23461 tacaaaaatg tgactagaaa gattgagtgt tactataaag caccttcaat tgatatggtg
    23521 caggagtggg aagatcgaat cactgaatgg ttagatatgt cgtcttatag tgattttatt
    23581 ctttactatg atgaacaata tatttatcaa gctgtagtaa tagaaggacc tgaatttaaa
    23641 ggaacaagaa aaacaggaaa tattgtccct tttgaattta cagtgagtat tcgaccattt
    23701 aaggaaaatt acaatggtag atttactatt cgacaaacag aaacttttga aatttataac
    23761 ccagagaagt attcttcaaa accgcttatt aaattgagtg gttctggaga tgcttctttt
    23821 tatattaata aggataaata tgatttgaaa tcgttggaca gagaattatt tatagattct
    23881 aaattagaag aggcttatcg aaaattagat ggtaatttag aacatcagga tcaggtcacc
    23941 ttatttttag attttccatt tttatatcct ggaaaaaatg aaattaaatg gacgaaaaat
    24001 attcattcat ttgaaataat gcctaggtgg tggagaaaag tatgaaacca agaatatata
    24061 gtcctactga aacagatttc tcaacgaatg gcttaggaat tttaaaggat tgtacaagat
    24121 gtggaatata tgaagtagct aatggaaaat atgaattaga gttggattat cctttaggaa
    24181 ctagatttga tgaatatttt gaaaatgact atcaaataaa agcaaagcca aacgatcaag
    24241 aagagtatca tatctttttt attgatgata aagatataga tactttttta aatacagtaa
    24301 ctatttatgc tcagagtcgt acaaatcgac ttggaagacg ggtagtcact cttgtggaag
    24361 tggactctaa aactggtcaa gaagcgatat caattattga aactaaaatg gataaaaaat
    24421 ctgacatacg actttattct gatattacgg ctgtttctag cacaatcttt gaagcgagaa
    24481 atgttttaaa ttgtattgct ggtgaacaag gatcattgct tcaatattgg ggcggagaaa
    24541 ttaaacgtga accatttaag ctttctttgt taaagcgaag aggtcgcaat aatgttggaa
    24601 cgattcggta tggaaaagat atgtctggtt tgaaggtcaa attagattgg acaggtataa
    24661 aaacaagaat tattccatat gctgatcctc aaagtgaagt aggtacgact aatcgaattt
    24721 atggttcgcc agtagatagt gcctatatta ataactatcc tgatgtctat acagaacatg
    24781 ttcagtttac agaagaacaa ggagtaaaag acattaagag cttaaataaa atagctaaaa
    24841 attactttaa aacgatcaat cctggctgtg ataagcctaa agtttctatt acggttgaat
    24901 ttgataa
//
LOCUS       DACDTH010000037        24437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDTH010000037 DACDTH010000000
VERSION     DACDTH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.037"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(56..790)
                     /locus_tag="IWK54_001643"
     CDS             complement(56..790)
                     /locus_tag="IWK54_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9593765.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="IWK54_001644"
     CDS             1117..1527
                     /locus_tag="IWK54_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9593766.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="IWK54_001645"
     CDS             1520..2221
                     /locus_tag="IWK54_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9593767.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="IWK54_001646"
     CDS             2221..3807
                     /locus_tag="IWK54_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9593768.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="IWK54_001647"
     CDS             3826..4047
                     /locus_tag="IWK54_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9593769.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="IWK54_001648"
     CDS             4044..4805
                     /locus_tag="IWK54_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9593770.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="IWK54_001649"
     CDS             5185..5694
                     /locus_tag="IWK54_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9593771.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="IWK54_001650"
     CDS             complement(5769..6308)
                     /locus_tag="IWK54_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9593772.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="IWK54_001651"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="IWK54_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9593773.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7375..8172
                     /locus_tag="IWK54_001652"
     CDS             7375..8172
                     /locus_tag="IWK54_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9593774.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8200..8835
                     /locus_tag="IWK54_001653"
     CDS             8200..8835
                     /locus_tag="IWK54_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9593775.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="IWK54_001654"
     CDS             8855..9628
                     /locus_tag="IWK54_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9593776.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="IWK54_001655"
     CDS             10036..10833
                     /locus_tag="IWK54_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9593777.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="IWK54_001656"
     CDS             10811..11224
                     /locus_tag="IWK54_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593778.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="IWK54_001657"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="IWK54_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9593779.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="IWK54_001658"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="IWK54_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9593780.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /locus_tag="IWK54_001659"
     CDS             16001..16561
                     /locus_tag="IWK54_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9593781.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="IWK54_001660"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="IWK54_001660"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9593782.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="IWK54_001661"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="IWK54_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9593783.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="IWK54_001662"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="IWK54_001662"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9593784.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="IWK54_001663"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="IWK54_001663"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9593785.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="IWK54_001664"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="IWK54_001664"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9593786.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="IWK54_001665"
     CDS             21908..22486
                     /locus_tag="IWK54_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9593787.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="IWK54_001666"
     CDS             22538..23020
                     /locus_tag="IWK54_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9593788.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="IWK54_001667"
     CDS             23137..23511
                     /locus_tag="IWK54_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593789.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23941..>24437
                     /locus_tag="IWK54_001668"
     CDS             23941..>24437
                     /locus_tag="IWK54_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP9593790.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT         8013 a         4391 c         5213 g         6820 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagtgcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcacccg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttgtttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacgac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatagaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcatt
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gatgaaaata aaataacaaa taatttgtaa agttaacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcatagc atgacagcat
    23641 tcggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa aaggtcctgc
    23701 tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta gggttgtttg
    23761 ttggtatgct tatataatag tcattagttg tgaatatttt gtgacgaaag ttttcgaaag
    23821 ttgttaagtt tttgagagta taaaatattt tgtgtaaatg aaaaaatcca tacaaaaaag
    23881 gaagtccctt ctgtagaata aagttaacga caaccaattc acagaaaaga ggacttccct
    23941 atgaatgatt ttactacaga aattgtgcaa actctagtca ctaaaggcga tttaaatgaa
    24001 ttattccgtt cgcacttaga aaaagcgata aacacactcc tacggactga attaacggct
    24061 tttttagatt acgaaaaata tgatcgcact ggttttaatt caggtaattc gagaaacggt
    24121 tcttactttc gatcaatcaa aaccgaatat ggtgaattaa cattggaaat acctagagat
    24181 cgtaatggtg agtttaaaca acaaacttta ccagcctaca aaagaacaaa cgatacattg
    24241 gaaaccacta ttatccattt attcgaaaaa ggtgttacga tgtctgaaat tgctgatttg
    24301 atcgaaaaaa tgtacggtca tcactatact ccacaaacca tgtccaacat gactaaagtt
    24361 ctgactgaag aagtaaatgc ctttaaatcc agagccttaa atgataagta tgtcgctatt
    24421 tttatggacg ctactta
//
LOCUS       DACDTH010000038        23261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDTH010000038 DACDTH010000000
VERSION     DACDTH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.038"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWK54_001669"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWK54_001669"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9593791.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWK54_001670"
     CDS             complement(1172..1867)
                     /locus_tag="IWK54_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9593792.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWK54_001671"
     CDS             complement(1880..2596)
                     /locus_tag="IWK54_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9593793.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IWK54_001672"
     CDS             2926..3534
                     /locus_tag="IWK54_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9593794.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IWK54_001673"
     CDS             3859..4182
                     /locus_tag="IWK54_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9593795.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IWK54_001674"
     CDS             4364..5734
                     /locus_tag="IWK54_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9593796.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IWK54_001675"
     CDS             complement(5864..7165)
                     /locus_tag="IWK54_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9593797.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IWK54_001676"
     CDS             complement(7519..9510)
                     /locus_tag="IWK54_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9593798.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IWK54_001677"
     CDS             complement(9654..9920)
                     /locus_tag="IWK54_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9593799.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWK54_001678"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWK54_001678"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9593800.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWK54_001679"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWK54_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9593801.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IWK54_001680"
     CDS             13264..13827
                     /locus_tag="IWK54_001680"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9593802.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IWK54_001681"
     CDS             complement(14080..14601)
                     /locus_tag="IWK54_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9593803.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IWK54_001682"
     CDS             complement(14604..15446)
                     /locus_tag="IWK54_001682"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9593804.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IWK54_001683"
     CDS             complement(15439..16164)
                     /locus_tag="IWK54_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9593805.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IWK54_001684"
     CDS             16340..16636
                     /locus_tag="IWK54_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593806.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="IWK54_001685"
     CDS             17204..18796
                     /locus_tag="IWK54_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9593807.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="IWK54_001686"
     CDS             18809..19549
                     /locus_tag="IWK54_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9593808.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="IWK54_001687"
     CDS             19717..21066
                     /locus_tag="IWK54_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9593809.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="IWK54_001688"
     CDS             complement(21194..21784)
                     /locus_tag="IWK54_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9593810.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            22020..23099
                     /locus_tag="IWK54_001689"
     CDS             22020..23099
                     /locus_tag="IWK54_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9593811.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         6661 a         4820 c         4527 g         7253 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaggcggat tttataatta acttagccat ttggaaataa aaaaacgcca
    21961 cgtgaattcc atgttatatt gaagttacca caactctcaa tagacaagga tgaattcaca
    22021 tgacgcaaaa ccatgataac acagtttcac gcaagggaaa acacctaacc tactcagaac
    22081 gttgccaaat cgccatcctg aaaaaggaaa actattccaa ccgtcaggtt gcggatgtcc
    22141 tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg cgggaccgtt actcagctta
    22201 accgccagaa tcagaacggg aaggtctacg actattatca tgacgtctac gacgccgatg
    22261 ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg cagacggccc ttatgggccg
    22321 acacggacgc cttcattgaa tgggcggacg acatgatgct gttggaaaaa tggtccccgg
    22381 acgtggtggt gggctttgcc agaaaacacg aattgttcga cccggccatt atccctggga
    22441 ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa aacgaagaat atggacctgc
    22501 tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg caaccgcccg aataaacggg
    22561 tgctagggaa atccattgaa gagcgcccgg aggcggttgg tacccggaaa accttcggcc
    22621 attgggaagt cgacacggtc gtaggaaaca agaccaagtc ggatgccgtg ctgctgacgt
    22681 tggtggaacg gcagacacgc ttcgaggtca ttctgaagct ggccgggaag gatgccgaat
    22741 cggtcaacca agcggtccga cagctgagag aacgggctgg tgcgtacttc ggccagctgt
    22801 tcaagacggt tacctcggac aacggctcgg agttcgcggg actgcacgat gccttgcagg
    22861 atgtggcaaa catctacttc agccacccct atgcttcctg ggagcgcggg acgagcgaaa
    22921 accaacacaa gctgattcgc cgtttcatcc ccaagggcag cccaatcggc aacgtgagcg
    22981 aaaaccaaat cttgcggatt cagcgttgga tgaacgatta cccgcgaaag attttgggct
    23041 atgcaacgcc gcatgacacg ttcgtccagg catttaggca ggaacggttg acggcttgac
    23101 acgtgggccc ttgacaacga gcctccctcg gcatggcttg tgtctggaaa gctggccggg
    23161 gccagccagt cgggagacta ccatgcctca gctcattgtc aagggcccac tcgattagaa
    23221 aaattagacg gttggtggta acgagcaacc gggtcaccat g
//
LOCUS       DACDTH010000039        23156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDTH010000039 DACDTH010000000
VERSION     DACDTH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.039"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(21..221)
                     /locus_tag="IWK54_001690"
     CDS             complement(21..221)
                     /locus_tag="IWK54_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9593812.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(674..994)
                     /locus_tag="IWK54_001691"
     CDS             complement(674..994)
                     /locus_tag="IWK54_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593813.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1497..2615)
                     /locus_tag="IWK54_001692"
     CDS             complement(1497..2615)
                     /locus_tag="IWK54_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593814.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            3086..4393
                     /locus_tag="IWK54_001693"
     CDS             3086..4393
                     /locus_tag="IWK54_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9593815.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            4514..5713
                     /locus_tag="IWK54_001694"
     CDS             4514..5713
                     /locus_tag="IWK54_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9593816.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5740..7008)
                     /locus_tag="IWK54_001695"
     CDS             complement(5740..7008)
                     /locus_tag="IWK54_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9593817.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(7178..7819)
                     /locus_tag="IWK54_001696"
     CDS             complement(7178..7819)
                     /locus_tag="IWK54_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP9593818.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(8228..8731)
                     /locus_tag="IWK54_001697"
     CDS             complement(8228..8731)
                     /locus_tag="IWK54_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9593819.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8854..9618)
                     /locus_tag="IWK54_001698"
     CDS             complement(8854..9618)
                     /locus_tag="IWK54_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9593820.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            9726..10001
                     /locus_tag="IWK54_001699"
     CDS             9726..10001
                     /locus_tag="IWK54_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9593821.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10561..11511
                     /gene="yidC"
                     /locus_tag="IWK54_001700"
     CDS             10561..11511
                     /gene="yidC"
                     /locus_tag="IWK54_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9593822.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(11557..11958)
                     /locus_tag="IWK54_001701"
     CDS             complement(11557..11958)
                     /locus_tag="IWK54_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9593823.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11972..12781)
                     /locus_tag="IWK54_001702"
     CDS             complement(11972..12781)
                     /locus_tag="IWK54_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9593824.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(12768..13658)
                     /locus_tag="IWK54_001703"
     CDS             complement(12768..13658)
                     /locus_tag="IWK54_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9593825.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(13671..14150)
                     /locus_tag="IWK54_001704"
                     /pseudo
     CDS             complement(13671..14150)
                     /locus_tag="IWK54_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(14326..15267)
                     /locus_tag="IWK54_001705"
     CDS             complement(14326..15267)
                     /locus_tag="IWK54_001705"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAP9593826.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(15264..16259)
                     /gene="lacD"
                     /locus_tag="IWK54_001706"
     CDS             complement(15264..16259)
                     /gene="lacD"
                     /locus_tag="IWK54_001706"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9593827.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(16252..17448)
                     /locus_tag="IWK54_001707"
     CDS             complement(16252..17448)
                     /locus_tag="IWK54_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9593828.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(17466..18194)
                     /locus_tag="IWK54_001708"
     CDS             complement(17466..18194)
                     /locus_tag="IWK54_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9593829.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(18635..19609)
                     /gene="lacD"
                     /locus_tag="IWK54_001709"
     CDS             complement(18635..19609)
                     /gene="lacD"
                     /locus_tag="IWK54_001709"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9593830.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(19606..20064)
                     /locus_tag="IWK54_001710"
     CDS             complement(19606..20064)
                     /locus_tag="IWK54_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9593831.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(20061..21524)
                     /locus_tag="IWK54_001711"
     CDS             complement(20061..21524)
                     /locus_tag="IWK54_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9593832.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(21506..22438)
                     /gene="pfkB"
                     /locus_tag="IWK54_001712"
     CDS             complement(21506..22438)
                     /gene="pfkB"
                     /locus_tag="IWK54_001712"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9593833.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            22580..>23156
                     /locus_tag="IWK54_001713"
     CDS             22580..>23156
                     /locus_tag="IWK54_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9593834.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         6955 a         4957 c         3888 g         7356 t
ORIGIN      
        1 tattttttag tataggttaa ttaagcttta gtaacgtttg ttgcttgagg tccgcgttgg
       61 ccttcttcaa catcgaaagt tactgcttgg ccttcttcta aagttttgaa tccgtcgcct
      121 tggatagctg agaaatgaac gaatacatcg tttccgtttt ctgcagtgat gaatccaaat
      181 cctttttctg agttaaacca ttttactgta cctgtttcca taataaaaat cctccttgtg
      241 tatcaacaca tattttgcaa ttacttgaat ggtgtatatc tggaagatgt ttatttgaaa
      301 caagctacca cagattaccg aacaaaaact actcctatag tatatcatac cgtttacaaa
      361 aaagctagag tttttcgact ttttttatta aaaaaagttt tctcagcgat tacataaagt
      421 gaaaaaccga actttttttt aaaataaacg acatattgtt cagttggtgt ttttttgctc
      481 tgatgttctt ttttaaacta aaaaaattat tctttctgac tacttatcgg ttctgtttca
      541 acggtcgagg ctggggaaga ttctttagta ggtctgatgt tctcatggta tttctgatag
      601 cctatgacag ttaccagaag aacaaggcaa accaatacga ccaacgagat tagtttttct
      661 tttttgctca tatctatacc tcataaataa caggatgatg tccatcatat ttctgagtat
      721 gagtaaaagc aaaagctgca gctgtagaat gtaagaaatg ccagcaactt tcataatcac
      781 aggtcggaca gtaccaaaag taaccaaatc ttggctgcaa agaccgttct tctgtttcag
      841 gaatcgcaga atcaagatct ggtgtctgta cataaacatt tttgtacaga cgtgtgcctg
      901 taataggaaa ccgctgtgtt tcttcagcaa aaactggctg acttgttaaa cttagggata
      961 gagacagcaa acaccataac agtctttttt tcataaagac ctccttatcg ataagttttt
     1021 cccaattaaa atcatatctc aagaacgccc gttattcaca ttttgttcat aaatgatgtt
     1081 ttttgtatca taatcgtcaa aaagaggtcg tgaccacgtc acaacctctt ttttaaacgg
     1141 tgttccagaa gtagcttctt cggaaataag ccgaaatttc acaaaaattt gagaaataat
     1201 ttttgtcaat ctcttcttat ttcttgaagc tgaccacttc tgtcacaacc tcttccttaa
     1261 acggtgttca aaagcttgca tctgcggtaa taagcccacc aaacaaaaaa tatgaagagc
     1321 tatttttgct tgctgttctt attacttgat gcagaacagc tttttcacaa cctctcatac
     1381 acggtgttta atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgtgaaact
     1441 atttttccgt ttgtttctct aatactcagg tcaaaacact gatttcacaa cctcttttaa
     1501 taacgaagaa tcgctgtcag actcttttca tctggcgcat ctttttgatc caaaataaac
     1561 acttttccgc cattaatgat cacatcatct gctaatgcat tcaacacttg acgccgatca
     1621 tattctgttt caggatcttc accgaaacca tcagctaagt ttgacgtagc gataaataaa
     1681 tgtgacactt ttccttcttt cgcagctgga acgatatcaa ctaactgatc gatgaatttc
     1741 ttatctaaga gtttattgta ttcttttgat tcttgagctg cgagttcact tgacagcttc
     1801 ttcgcccctt cttcgatatc tctaaaggat agctgcgcag gagaagcaga aatcgcaacg
     1861 tctgcacaat aatagggatt tttagcaatt tttttgaaca tcgtctgatt ttctggtaga
     1921 gcaaataaat aaatcggcca ttgttctgga ttttcgaaag aatccttcaa gtacgtatca
     1981 actgcttggt aatagttgac ccaatcgatc tctacttctt catcttttgg atttacgcca
     2041 tggaaagtta ctccatctcg ggctccgcct cctccattag aagagaagtt caaaccgcca
     2101 cctgtcagtt cagatcccaa tgcagtaacg acatcaaccg gtgccccttc tggtagagat
     2161 acttctttta cttgtttgtt gctgacttca tacaatttca tggagtcgcg gttcaatccc
     2221 aataaataat aagaatagtt gaactgccca tttttgataa tagccagtaa ataaggaaga
     2281 tcacttacat agtattgatc gtctaccggg atactcaaac gatggatata ggtttggtca
     2341 gcagttataa tgaccgctac actcgtagtt gcactacgcc aaaacgaagg atctcctaac
     2401 aaattttcaa ttttttcttg gaacggggac cagtcatgtt cggaatattt tttctcaaaa
     2461 cgtttctttg cttctttcgc aaaatttttg aatttgatct gatctttttc cacatcttga
     2521 tgggcgacat gcgtattcag catgatcgtg acgaatggtc ctttagcgcc ttctgaagtc
     2581 aattgtgaca ataattcttt atcctttttt gccataaacg tacccctcct aaaaaaatca
     2641 ttcctttgtt tctatctaaa gtctaccaca acactatttt tccatacaat aaaacgtatc
     2701 taaaaaaata aaacggtttc tacggtttta aatttagcta tttttttaag ttatcttcat
     2761 aatttcctca attatttagc cccagtcttc aactattctt catgaaatgc tgataaacta
     2821 agaaaaagaa taaaagaggt ggatcttatg catttgatta tgattttcag tttattaagt
     2881 attatcagtt tatttgcttt cagcgcttcc cgcttattag atgcggaaac acagcctatt
     2941 cctgtaaaag tacgtaaaaa cgaccgctaa ccttccataa cgtgcgttct tatcctaaag
     3001 ttttttcaga aacattgaaa ctctctcccc tctctgataa aattttaatt tgtagcgaaa
     3061 caaattaatt acaaggagat cggatatgtc cataaaaaaa acacgtctag tcctattatt
     3121 ggctagtctc attctttgcc ttgcttcttt tctaccagct gttgctgtgc aagctgaaga
     3181 tactttcaaa gtaaatgcaa aagcagcctt tgctgtagat gcgcaaagcg gtaagattct
     3241 ttatgatcaa gatggagaaa aaaccatggg gatcgcttca atcacaaaaa tcatcggtct
     3301 ttacatcgtc cttgatcaag taaaagaagg gaaactatca tgggatgata aagtttcaat
     3361 ttcagattat gcagaaaatt tgagtgtcac cccagattta tcaaatgttc ctttgcataa
     3421 agaaaacacc tacaccgtta aagaattatt cgactctgca atcatccagt cagccaacgc
     3481 ttcaatggtt gccttggcag aaaaaatctc tggcagcgaa gcaaaattca ctgaacgaat
     3541 gaaagaacaa ttgaaagatt ggggtatcaa agatgcaacg atcgttaatg catcaggact
     3601 gaataactct tatcttggcg aaaaccgacc agaaggtact ggagaaaacg acgaaaatca
     3661 aatgtctgct caagatgtgg ctatcgttgc ccgtcatttg atcttggatt ttccagaaat
     3721 actggatgta tctagcacta cgacacaaat gtttggcgaa aacacgcaat caccagtaga
     3781 aatggtcaac tggaactgga tgctcccagg attcgtgaat tataaagaag gcgtggacgg
     3841 attaaaaact ggtacgactg aacttgcagg agcctgtttc gtcggtacga tcactaaaaa
     3901 cgatcaacga atcatcactg tcgtattgaa tgcagacggc catgcggaaa acccaagtgc
     3961 ccgtttcaat gaaacagctc gcttaatgga ttattgctac gacaattggt cagaaaaaga
     4021 gcttggaaaa gccaatgcaa gtatccctag tctaaaaacc attgatgtaa aagacggaaa
     4081 agaatcaaca gtgcccgttg tgcttaaaag tcctgtaaaa gtatgggtac ggaacgatat
     4141 ggatactgga aaattaacaa tcacaccaac tcttgacaaa aaacaagtca ctgacaatga
     4201 gatccaagca cctatggata aaggaacaaa aatcggtacc gcaacgatca ccttggcaga
     4261 agataaatta ggttatcttg aagacaacaa cgcgccatcc acagaaatca tcacggataa
     4321 aaccgtagaa aaagcaaata ttttcgttat tggctggcga cgcgtcgcag aatttttcgg
     4381 taacttgttt taattgaaca aaaagaaaaa agcatctttc catgtggaag atgttttttt
     4441 gctttttcta gtttaaataa cagctttttt catctatttt cgttatactg tataagaaaa
     4501 aggagttgaa tttatgaatc taaccagaga tctgattttt ttggttctct atcttattgg
     4561 ggtattctcc atcgtttatt ggcgaaaaca gaaaaaggat ccttccttgt tcttttacag
     4621 cagtacatgg acgtttgcgg taatcttgac ttattttgtc gtgttggaaa ttttttattc
     4681 gaccttttat tttttgattc ccttagtgtt tttcagtatc tttgcctttt cctatttttc
     4741 agaaaagcga aggctgttga atggtctttt attcaatatt ttcctcatta gttttggtat
     4801 ctacttgttt gtcttgctat acgaaacaca aaatattttt cttggcggac tgattgcctt
     4861 gatcacgatc ccgttactgc ttgtctttct attcggcatt tatggattga tcgtttttct
     4921 tttttggaac ggtgtcacag ttttacgtag agagtcgcat tcattagcta atcttttaac
     4981 gttgatacta gccatttttc ttacattgtt tcttgttttt gactttttct tgttaaaata
     5041 tttaccacaa tggataaatg ctttgttttt ctgcatccca ttgatcttga tctatctatt
     5101 tatcgtattc tacaactttt tgactgtctc attcttgtat cagttcaacc gccctcgata
     5161 caaccaagac ttcatcgttg tgttaggcgc cgggttgatc aatggagaaa cagtttctcc
     5221 tcttcttgcc aagcgaatca ataaggcgat tgctttttat tgggcacaaa gccgtgcgac
     5281 attaaatccg cctatcctat taatgtcggg tggacaggga gcagacgaaa aagtaccaga
     5341 agcaatggcg atgaaacaat atgccatgga acaaggaatt cctgaaagag atattcttgt
     5401 cgaaaccaat tcgacaacca ctttggagaa tatgcgctat tcaaaggaaa tcatggatca
     5461 gcaaatgaaa ggtccctatc gcgcgatctt cagttccaac aattaccaca ttttcagagc
     5521 tggtctctat gcacgacaag cgaaactgaa ggcaaatggc attggggcaa aaacagcttt
     5581 ctattacttg cctaacgcgt ttttaagaga atacatcgcc atcttgatga tgaacaaaaa
     5641 aagacatatg atcatttgcg gattgctttt cgcaggttcc gcattcttat cactgatcac
     5701 tttacttttc taaaaaaaca aggtgtgaca aaacatatgt cacaccttgt ttttttcttt
     5761 tattctcttg acgattggca agaatagtat tccttttttg acggctactt gctgtgcaga
     5821 ataaataccg ctgtttcctg aacacataaa gctgatcaag cagatcataa agaagtaagc
     5881 agcagcttgt gaaccaaata attcgattcc catcacaaaa caggcaatcg gtgtatttgt
     5941 cgcaccagaa aataccccga taaaacctaa tccagcaaga aaaggaacag aaacatggag
     6001 caaaggcgct agtactgcgc caagcgttgc accaatctca aatagtggtg tcacttctcc
     6061 cccttggtac cccgcaccta aagacaagac cgtgaagaat aatttcccaa taaaatcata
     6121 agcatgtgcc tgtccattaa atgattcctg cagtaacgga agactcaacc caaggtaacg
     6181 ttgtgtttgc aaaatcaacg ctgcggctac aactactgct ccacctatcg tatttcgcca
     6241 aacaggatga ggaatccatt gcgcataccg cgcttttaaa aagacgatcg aacggctgaa
     6301 tacccaacca atcaaaccaa aagcaatccc agcaacaaaa actttgaaga acagcaacat
     6361 actccattca ggaatcacac ccatttggta atgtagatga gaaacaccat agctttctgt
     6421 catgaaattt gcaaaaagcg cagcaaagaa gctaggaaat attgcttctg ttcggacttt
     6481 accgattgcc aacacttcta atccaaataa tgttccagct aaaggtgtgc caaatacaga
     6541 actaaaaccc gcactgattc cactgataac caaaatctcc cgttctgctt tatccaatcg
     6601 gaataaatgc gctatattat cagctaatgc tccccccatt tgaactgctg ttccttcacg
     6661 accaacagat cccccaaata aatgggtagt gatggtccca aacaaggtca atggaatgag
     6721 ccgcaaaggg atcttctctt ctcctccatt cccttgatcg atcactaagt tattcccacg
     6781 actagaaaga ccgccatgat acgcataaag ataagcaaaa agcgcgccgc tgactggcaa
     6841 tagataaagt agccaaggat gtgctagtcg gatatctgtc acgaacgtca ggctatttaa
     6901 gaaaaaagca gacaagctcc ccatgaatac tccgagaatc gctgtgacca ccagccactt
     6961 caacatgtac atcaaaatac gcactggact cggtaattgt ttttccataa aaaattcctc
     7021 ctcaaataaa aaaacgccta ctaaagaaag gggctggccc cagactttag taggcgtcat
     7081 tagctatctt acgttagcat ttaaaggcga acaccatcac ctattcactt ccattaaaag
     7141 atagcatatt tttctgtaaa aaacaataaa attatcgtta tttctttaca cgttcagcaa
     7201 attcatagaa aggagccaat ttcgttaact gttgattaca ttgatcacta tcatgacaaa
     7261 tataattccc ctttttcgta tacgtgccat cagaaccggc tttcgttgta gaaagaaaga
     7321 gcgagacatt cgatgttttc tgacagatcg cacagattcc cttattaatc gttggtgata
     7381 aagtaccgct gatccctttt aattttccat cttcgtaata aaccatgaat tttttttgcg
     7441 tccctggatc attccaaccg atgtacgtat gtgcttgcaa gtcgatttca ttccaagcgg
     7501 gttgtttgag ttttttcact tttcggaata atttctcgat ttgtggtttg ctcggctgca
     7561 taaacggctg gacgatttgc ttcagctcct ccaatgctcg ttctgcttgc gtttttgtca
     7621 gttttacatc taaaactgtc gttaaaaacg tatcgatttc tgatggttcg taacctaata
     7681 catcgcgaat cttttcagct gctagagcct gaaccgtttg taccgttttt gagtcattga
     7741 cagatgagta cgcatttaat aactgctcta cttctctttt tataaaaaaa tattgatatg
     7801 gtttcattgt ttgttccatg attttaaagt cctttcttct aaccaaagtg catactttgt
     7861 agaaaaaagt gatcttctgc aaagtatgtg ttacctgaag atcccggact cttttaaata
     7921 agatacgttt tgcatcttat catcaccccc tttaatgttc attcttctat gtacatgatt
     7981 cattcgataa ataaaagtaa accgtatctt gagcagatag tcaactgcaa acagaaaaag
     8041 gactgtgaca aaagtcgtgt caaagtccct ttctacaaac aaacggtgtt ccagaagtag
     8101 cttcttcaaa aaataagccg gaatttcaca aaaatttgag aagcaatttt cgtaaattcc
     8161 ttcttatttc ttgaagctga cccacttctg tcacaacctc aatatggtct attattggtt
     8221 ttacctgtta aatcgaggat tcttttccga agtaacgttg ccatctctta cgcatacttg
     8281 ctgacatagg ctgagcggct tctttgactg cgcagtattt gtctacgaat gttacgatat
     8341 atccttcttt gtacttaggc ggtgcaatcg ttgcgcccca catgtgcttc aaaatgatat
     8401 ctcgctcaag atccgacaaa tcggtgatct tttctgcatt ctttacagcg attcttggat
     8461 ggatatacgc atgtgtgcct tcatcaaatt tcgtcgtacg ccaatcatag tagaacagat
     8521 catgcaaaag tccagcacgc gctgttgctt tagcattgcc accccatttt tttgcgagca
     8581 gataactata gtaagaaaca ctgatggaat gctccaaacg agtggaatga acatgctgtg
     8641 tgtaattcgc taattttttc actgcttctg tttctaaaag atcttctaca taagacatat
     8701 attcttcgtc ttcacgccat ttttctgtca taattatcga tctgccacct ttttgaattt
     8761 cattttcttc tgaaattgat ctaagtatac caccagtgtt cggattattc tagtcgcctt
     8821 ggataaaata gccaaagctt aatagttcct taatcataaa gaaaaatgat aaagaacaat
     8881 ccctgccgca accccaacat tcaacgattc cgcatttcct ttcatcggga tatatacgtt
     8941 ctgatcagtc aaagacaaga tttcctgact taccccctga ccttcgttcc ccatgattat
     9001 tgcgaagtta tctgattttt ctactttgtc taaggcgtgt gcttcttcat ttaattctgt
     9061 gccatacact gtaaaccctt tgtctttcat taaaggaatc acttctgtca aatctttttg
     9121 caacacaggc aaatgataat tgcttccctg catgctgcgc agcacctttg tactataaat
     9181 atctgccgtt cctttgccta ataaaacacc agccatatca aatgcatctg ccgtacggat
     9241 catcgtccct acatttccag gatcttgtac attatctaat aacagccatc ctcctgaaaa
     9301 atctggcgtg ccttcagccg ttaacgccat cactgctaag attccttgtg gtgtaggtag
     9361 atccgataca gcatccatga cctcatccga aactagaaaa gtagaaatct ctttgtttgc
     9421 ggccagccag tctccccatt ctttcatgcc tcgctgtgtc acaaaaagtt cttttatttc
     9481 tgcatcagac gacgcagctt cttcaatcag atggaaccct tccaaaagat attcttcctt
     9541 ttcttcccga tatttctttt tatgtagttt ttttaattct ttgattcttg gattttttgc
     9601 tgattggatc tctttcacat tcctcacctc cccatgatta tatcatggaa taggtgcttg
     9661 agcttgtttc atcctaaaaa aatcggtatc attaatttat aaaataggaa agaaaggtga
     9721 cgaacatgcg caaagttaaa atgaacgtcc aaggacgcgt ccaaggcgta ggatttcgct
     9781 acatgaccaa gatggtcgct gatcaactag gcgtgactgg aacggtaaaa aacgaagatg
     9841 acggctcagt cacgatcgaa gcaatcggca atgacgacat catccagaaa tttattgaag
     9901 aagtcaagaa atctccgagc ccaagtggac gagtgcagta tgtggatgtc caagaagacc
     9961 cgatgattga ggaacgaaag aaatttgatg tggtgggata aggttgtgaa aaagctggtc
    10021 tgcatcaagc attaaggaca ggttgtgaaa aatagctctc catatttttc aacaggctgg
    10081 acttaatgcc gtagatgcaa gcttttgaac accgtcaata aggttgtgag aaagtcgctt
    10141 agttttgagt agtaaggaga attttgtgaa aatggctttc cacatttttg cataaaattt
    10201 gacttaatac cgagaaactg acttttgaac accgtcgata aggttgtttt tgagacgctt
    10261 tgcctcaagt attaaagata ggtagtaaaa aagctgatct ataacttgca ataaatttgc
    10321 ttaacccata ttttccagac agaacaaaaa agtttttagt tttttaaata agaaagaaac
    10381 acttggaatc actcttttct ttccaagtat ttctttcttg attttttgtc tttttttcaa
    10441 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
    10501 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
    10561 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
    10621 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
    10681 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgagaattt caattggtct
    10741 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
    10801 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
    10861 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
    10921 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
    10981 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
    11041 acagaaggta ttcgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
    11101 cttgttgtca ttgcaggggt cgcctatctg cttcaaggat acatctcaac gatcggtatt
    11161 cctgaagaac agaaaaagac gatgcgtacg atgctaatcg tctctcctgc tatgatcgtc
    11221 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    11281 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    11341 gcagaagagt tgaaacaaaa cccgccaaaa caagtcgtca ctccaagaaa agacgtgaca
    11401 gaatcagcaa agccagctaa caacaaacaa gcaacaaaac aaatcaaaca accaaaaaac
    11461 agtacaaaca acaaaggccg caacgcagga aagcagcaga agcggaaata aaaacatagg
    11521 acaaaaaaga tgagagctaa gccctcatct tttttgttac tcttcctctt ctgctattcc
    11581 attaattaat tcgttgacag agacaatgct ttcacatcct gcacgaacaa actcgggctc
    11641 tgaaccaatc atttgtgagt tgataaattc aatgaccatc ggaagattca tcccagcata
    11701 aagagtaatc tgttgtcctt ccattattag tttagaaatg atatttgcag gtgtaccacc
    11761 taaaagatcg catagtacca caaaatcatt taacgaatct gtgacagtga gaaacttttg
    11821 tttgtatgtt tcagtgccat cttctggtaa taaaggtaac gtgtagatat cctcttgagg
    11881 ccccatgatc atttctgtac tttttttcaa ttcctcacaa aattttccgt gactaatcaa
    11941 taccaattgt ctactcattt ttcgctctcc cttatttcga aatcacaccc aatgcagata
    12001 aagcaacaca cacaattaat acgataaaaa tcgctcgagt aggagtcatg tttttctttc
    12061 ctaataacca ataaacacca ccgacgatcc cagcaggtaa aattttaggt aagatcatat
    12121 cgatatagtt ttgcatattc aatgtcacat cgccaatcgt tggtgcccat gaaaatttaa
    12181 cattgatcat tgtcgcaact aatgccccta ccatgaatac ccctaataaa gtggctgcgt
    12241 ccgttaatgc agttaagcga gattgcatag tagtaactaa ggaaattcct tctttgtatg
    12301 caaattcaag ttgcttccaa cgaaaaacca tgacaacgat ctgagcaaaa atccatataa
    12361 acacacctat cggatttcca tcagttgcca tattcgccgc caaagcccca aaaatcgttg
    12421 gaatcaaagc agcaaatacg gaatctccaa tcgctgcaaa ggaacccatc aatccagctt
    12481 tcagccccga tacggtttcc tttgaagcaa ttccctcttt ttcttccatt gcaagatcaa
    12541 tcccagtaat gattgtatta aaaaaattag aagtattaaa aaattgcgta tgtgttttca
    12601 tcatctcttt taattctggt gtattgtctc catatatttt tcttagttgt ggcaagatag
    12661 tatataaata gccagaaccc tgcattctct catagttcca acctaattga aaagtgaaca
    12721 aacttcttcg attgatttgc ttaaaatctt cttttgttaa tttgtaatta gatttcgtca
    12781 tcttcgattt ccccctcaac agaatgtgat ggctcactag ctaatggttg atggtctttg
    12841 ttttgatcat tcttataact gatcattgct agtgctagac caatcaatgc tacagcaagc
    12901 atcggcagcg aattgaaagt tgccggaaga tctttaacaa cgcttgccac acttgttcct
    12961 aataattgaa cattagagaa taacgttgct aataatgccg tgatcgtaaa tcctaaaata
    13021 agataagata gatgtttttt tactggtaaa tagcgcagaa gaatggcaaa cccaacagct
    13081 ggtaaaacag ctcctgctac tgaaagtcca tcgcctagcc attttagatt cccgtttaaa
    13141 acagaaacaa cattttcaac taatccacca ccaaatgcta aagctaaaaa tacaggaatc
    13201 atacgagaga gcgaccaagg taaagctccc attaagaaat tgcgttcgat tgcttgataa
    13261 ttcatgtctt ctacgtgacg gtcaatacga tgggcaaagt atgtgttagc gaatctagct
    13321 aaaatatcca attggatcat taagcttgct acaggcactg caatagcagc aatcgcttgt
    13381 tctggattca ttccaagaga aatcgagaag gcagttgcta aaatcgtccc agagttggca
    13441 tctatttttg atgcgcctcc aaacgtcccc actcctaaaa ctgtcaactg catactgcca
    13501 ccaataatca aacctgcgtt taaatcaccc attactaaac ctgcatataa ccctgcaaac
    13561 actggcgcac tcattgaaga atagatctgt aattcatcta aaatttgata acctgcatat
    13621 aatgttaata ataagatctg ccaccattga atatccatgg atcattcctc ctatttgatt
    13681 aaaagattca taaagtcttc tgctttatcg tttggtacca tttgagcaat taaatgtata
    13741 ccaagctgat ctaatttttt aaatacttcg atatctgcat ctaccacatt gattgatttt
    13801 gtaatggacc gtgtttcatt cgtttgtgac atattcccca catttacttc tttgatagga
    13861 actccttttt ctactaaaga taagattcga tctggctttt ttgctacgat caatactcgt
    13921 tgagagtcat atttgccagc taaaatattt gttgctgctt tttcgattgg caaaatgctt
    13981 aattttaccc ctgctggagt cgctaacttc aatccacttt tttcaattgt gtttgtggct
    14041 acctcatcat ccacgaccat gattcgacta atattcagtt tcgttgtcca aagatttgct
    14101 acttatccat gtatcaaacg tccatcgatt cttacaccaa taattgccat catgcattct
    14161 ccttatctat tttttctatt tattgttcaa ttaaaactgt gacatcatca aatatccctt
    14221 ctgtttcaaa gataacgttt tcattttttc tttcttatat ttcattttta agctaagaac
    14281 aaattccttc ccaactaaag aaaacgcttt cttttatatt taagttcatt tgattttctt
    14341 aacttgaatg tgtttcatca acatcgtaac tttcataggg tcgatccagc cagttttctc
    14401 ttgttgcgcg tttgccatac cacaagccat agaaaatgcg agaatctctt cgattgattg
    14461 cctttttgat aacgcatggg ccgatcctgc aagggtcgcg tcacctgaac caacaggatt
    14521 aacaactgat attttaggaa taatgacttc gtaaaaacac tgagcaactt ttgccacagc
    14581 acctctctct cctaaagaaa caacaaccca tggcactcct tcaaatattg gatgagctaa
    14641 taaggactga attttttcta attgaatcga agaacagata cgttggccaa tcagttctcc
    14701 taattcttcc agattgggct tgataagtag tggcttctcc ttaccttcca atacttcaag
    14761 caaaaattta ccagaagtat ctagtaatat gtctttccct tttttatcag ctaaagcaac
    14821 caattgctta tagtaatcaa gagggcatcc ttggggtaaa ctacctgaga tcgtcataat
    14881 atctgatatt tcgaggagct gctcgaaatg atcggtaaat gtctgttgtt ctttctttgt
    14941 tatgaagggg cctcgttcca aaagctctgt ttgtctcgaa ctatgcagga tagcaataga
    15001 atcccttgtc tctcctttaa ttggaacaaa ctgatgcatg atccctaact gatctagctg
    15061 gttctttaga tattcaccgt gctggccacc tattaaacca gtagctatca cctcttctcc
    15121 taataaattt agaaccctcg ccacattcac tccttttcct cccgctgttt tattgactgt
    15181 ttcaactcga ttgactttgt cgaggtttaa ttcttcaatt gtatacgcga tatcgatgga
    15241 aggattcaag gtaactgtcg taatcatttc atcttcacct ttttgctttt tgttttattg
    15301 aaccaagggg tagcggtttc atttattact ttatttaaag tctcgatgtt ttctctccca
    15361 tttgttctta accaatgaat agctccgtct tttccatttt cgacgtatgc tttgacccca
    15421 tctttccatg tcgcacgtcc acacaataca ccattgaagg tggaacccga ttcatgagca
    15481 aaaatcagtg tttgttgaaa taattctgtt gaaacacccc cactcaaaaa aatgaatggt
    15541 aatggagtcg cttcactttg ctccttaaag aaggcagcag cctcttcttt gctgtgaata
    15601 aattcacctg tggaatagcc attcacataa ttcatattga ctggtacttc tactttcaac
    15661 acatcaactt tatattttgg atcagaaaat tctttcatca tctcaataac tttgtggggc
    15721 ttgactttcg catactctgg agaagaacta tcgcttattt gtgcatcata agaaactaac
    15781 tccaaataga agggacgatt ttctccttcg cactcactac ctaaacgttc gatgaacaca
    15841 tgcttcaaat gattgatttc gggtgcttca tcaacatcgt agtaaagtaa gaattttact
    15901 gcatccgctt gctgttcttt caatctgaga gaagaccaat ccgctaataa atcaggtaga
    15961 cgaccaggcg tagttgcgtc atagccggtt ttctcataag ctaacaataa ccccgtctct
    16021 tttgctctga cttttgctgc tggcaatcca tattccggat ccaataaaat agcagaagca
    16081 tagggtgtca gctcagctga tactagttct ttgaattcac tgatttggtc ttcacttggc
    16141 tcttttccca acgatttgat catttttttt agagcaccac gctgatcgat cgctaatgca
    16201 ctaataatcc catcttttgt tgatagtctc tccattgccc tatttttttc ttcattcatc
    16261 tgtcatttct ccttcggttt catacttatg aataatgacg cccttgacta ctcgattgac
    16321 cgtgcctgtt ggagatggtg tatctggttt attagctact ttgattgaag tatagagtgc
    16381 aatgatttgt gcagtcataa tcatcgctaa tgcaagatag gcatcgggca atttactcac
    16441 cgagtcttcc aatagcacag tcatacctga gaaatcaata gtacccggtt gcgcaatcgc
    16501 aatgacattg gcagtgaccc gatcttcttt tatttcctct aaaatatcta agtcataatt
    16561 tcgcgtatat ggttgattgc tcacaaaacc tacaacgagt gtctcgctat ttaaaaaaga
    16621 ctttggtcca tgacgaaacc ccatcgaaga atcaaaaatt gtagcaactt tacctgctgt
    16681 taattctaaa atttttaatt gtgcttctct tgtcgcacct gccaaacttc ctgaacccag
    16741 ataagcgatc cgttcaaaaa aatcaggtaa taacttttga atttcttgat ctcttgaaag
    16801 aagatcttga ccgattttaa ctaattgttc aagatatctt tcttttttct ccattgctgt
    16861 ttgatcaaag atccataaag cactcaataa catacacgta aaacttcctg tcatagcaaa
    16921 acctgcatcg tttgctctct ctggcatcaa taacagataa ttctgtttat catcctgtgc
    16981 gttttttgcc aacatcccct ctttagcaca agtaatcgtc aaatgaaaca atgtttcaac
    17041 ttgttcattt gcaatttgca cagccgctac actttctgga ctgttgccac tgcgagcaaa
    17101 tgaaactaat aaagtcggtt catttggaaa caaaaattct tttggtgaag ctacaatatc
    17161 tgtcgtgcca aaactgtgga agataaaaaa ctgattgtct ccatgttcat ttagataggg
    17221 taagagcgta tccccaatat attgagacgt tcctgcaccc gtaaaaataa cacgtattct
    17281 tcttccgttt gcttcttttt ttatttgatc aaaaaataga gctaaatttt cttggctttg
    17341 catcaatcta tcgattgttt ctttccagag atctggttgt tgttttattt cttgtgtcgt
    17401 gattgctgca cctatttgtt ttaatttgtt cttcgatagc tcaaacatgt tgttctccct
    17461 ctcaatcatt ttcggatatg ccgaatttta taatgaaact ggtccgctct tgctacacta
    17521 tctgtatatt cgataatctc atttttcacg ttataggtta tcctcgtcag atgtaagacg
    17581 ggagcgccat cagcgattcg tagcacaaca gcatcttttt tttgcacgat acttgcataa
    17641 aactcttcat cagctaattg aacttgttgt tcataatcct gtgaaaacac atcatacaac
    17701 ggtttacttt caagtaacgg caaatcaagt cccataaata gtttcgccgg tagataagaa
    17761 cgctcaacca tcaatggctc ttcatctgcc aaacgcaatc ttttaagttt aaatacagtt
    17821 tcacctaaat ggatgttcaa attcttagca attctcttat tagcttccat ttgtctaaat
    17881 tctaaaatga ccgtcttagg gacgcgccct atttcgcgca tttcttctgt aaagctatat
    17941 gctccagcta agttcgatga cttatcagac aaatcagaga cataggtacc ttttccatgt
    18001 cttcggtaaa tataaccttg attttccaat tcctgtaaag ccagacgtac ggtggtacga
    18061 ctcaccccat agtgttgaca aagttctctt tcagataaca gcttatcatg cggagtcatc
    18121 tgtgtttcga tacgatcctt caataaatca actagttgat aatacaatga ttttccttta
    18181 tttgagctct ccataaaaat cacctcttct ttactggtta taaccacaac acaagaatag
    18241 tgaattatta atcatttgtc aaaggttttt tagaccaaaa aagctattat aacagctctt
    18301 taaagcttta tatcttgaat agacaaagat acctcaaaaa taccagttac caataaaaga
    18361 acagaaaaaa agacgaacca cataagcgat ccgtcaaaat ggtctaactt atcgagcagc
    18421 gtgatccaca ttaatcccta tgatattcct acaactatcg ttttattact tccaatatca
    18481 tttacaacaa ctaaaaatag cttattaatg acaaatgtta ttattagttt tagataactt
    18541 ttgcagtttt atactttttt tctataaaat ttgacaaaca tttttttaaa taactaatta
    18601 ttttttgaat ctgtcactca gacaatcctt tctcttactt gttaacataa atagaagttg
    18661 ctgtttcttc taatagtgta cttaattctt taatattttg ttttccttct tctaacatcc
    18721 aactactcgc tttttcttcg ccttcggtaa caaaaggtgc tactcccttc gcccatgttg
    18781 ctcgcccaca taagacacca ttgaatgtcg aacctgattg ttttgcaaaa cgcaatgtat
    18841 cttgaaatag tgcagcgctg actcccgcac ttaaaaaaat aaacggttgt tcagtcactt
    18901 tcgattgttc atagaaatac gcggatgcct ctgcttgact ataacaagta tcttgcccat
    18961 atccttccac aaatctcata ttcactggta cttctacttt caatacatcc acttgataac
    19021 gttcttttga aaattctcgc atggcttcaa tcactttatg cggttttact tttgcatagt
    19081 caagagaaga cggatcgcca atagttgcat cgtaagaaag gagctctaaa aagaaaggta
    19141 tgccttctgc ttcgcattct gaaccaatcc gttctataac tatttgtttt tgcacattaa
    19201 tctctactgg ttcatcaacg tcatagtaca ataaaaattt acaagcatcc gcacccactt
    19261 cttttaacct tttcactgac caactattca gtaaatctgg tagtcggcct ggcattttcg
    19321 catcatagcc ggttttttca taagctagca acaaaccaca ttctttctct cgaagcgctg
    19381 ctgcaggcag accatattct ggatcgagta aaatagcgga agcatacgga gtcaggtact
    19441 gagaaaccat ctgtttaaat tggatgatat cttctgatgt agcttcttta tattggttga
    19501 tcattttctt caaagcacca cgttggtcga tagctagcgc gctaataatt ccagcttcat
    19561 ccgtcaatcg cttcaaacgt tcctgttttt ctatactcat tttcttcata tatcttcacc
    19621 tatttcttta gttaatattg tagctatttc tatttttgat tttgcagctc tcaactgttc
    19681 tgtaacctct tttcgcatca atgatcgtgc aacgagagac aataatttca aatgagtcgt
    19741 tcctgcttct gaatctggga taaataacgc aaaaataaaa tcaactgact gtctatctaa
    19801 actgttccat tcaattcctt ttttcagtgt gacgataatc aaactaggtt cattgattgt
    19861 ttgagacttc gcatgaggga tagcaaaacc tgacattatt cctgtagtac cttctttttc
    19921 tctttccaga agtttttgat atacttcttt ttcgtcatga gcgattccta aacagaatgt
    19981 ttgctttgct aaaaacttaa atgcttcttc ttgattatct aatggctgtt gtaaaaaaat
    20041 gttctcttca tgaatgattg tcatgataaa cctcctgctc ttccttcttc gataactgta
    20101 cgatttgttg ctttattatc cctcttcttg tctttatcag ttactctttt cttttggatc
    20161 aaacctagta tcactccacc aacgatcgaa ccaataagaa tcgctgtaac ccattggatt
    20221 tttcccgtca ctacaggtaa cactaaaaaa ccgccatgtg gggctggtac ttggacacca
    20281 aacagatagg tcaagacagc agcaattgat gagcctaaca tcatcgttgg taatactcgg
    20341 atcgggtctt tcgcagcaaa cggaatagct ccttccgtaa tatgagttga tcctaaaata
    20401 aaattaacca aacctgcatt acgatcatta gtatcaaaat atttaccaaa caataaagta
    20461 gcaaaaccag taatcaacgg tggtgcaatg caagctgctg acgcaccagc cataaaagtc
    20521 gtgtttcctt gtgtcaaaag agctgttcct gttacatatg ccgccttatt aaccggtcct
    20581 cccatatcaa acgcacacat acagccaaca atcacgccaa gaacaagtgg attcgaatat
    20641 tcaaatccag ctaaaaagct catcattcct tcatttatag ccttcattgg acctgccagc
    20701 aaagacatca cgatgccaat aaatgctaca ccaataactg gatataagac gattgccttc
    20761 aagccatcta gttgtttagg taaaattatc agtagctttt gcaaaaataa gataaaataa
    20821 cctgctaaga acccagctaa aatacctcct aaaaatccag taccgccatt catggctacg
    20881 attccaccaa caaatccaac gactaaccct ggacgtttcg cgatcccttc tgcaatataa
    20941 gctgctaaaa ctggaaccat cattcccatt gctgcatcgt caactgcttt taccattgcc
    21001 gctgtagcat tgtattgtgc atgatctgga tcagaagaat agattcccca aacggcaaat
    21061 gaaatagcaa tcaatactcc gccagctacc acgaaaggca acatatgaga gacaccgttc
    21121 atcaaatttt tatagatgct acgaccaaaa ttagcaccac ttatcgcttc tggttctaaa
    21181 gtatcagcag cttcattatc tgctaagaca gtaccttttc ctgccaatgc ttcgtcaatc
    21241 agccgttgtg catccttgat tcctgtactg acagaaacat caatgactcg ttttcccaca
    21301 aatctttctg aatggacatc tttgtctgcg gcaataatga cagcatctgc ctgtttgatt
    21361 tcttcagccg ttaattcatt ctcgatccca atttgtccat gggtctccac tttgatcgtg
    21421 atgccttttt tcctagctgc ttgctctaat gcctcttgcg ccatgtaggt gtgcgcaatt
    21481 cctgtcggac aaccggttgc tgcaattagt tgatattttc ccatctttcg aacctctctt
    21541 ccttgatttt gatttgttta cttaattcct ccacatccaa aaaatcagtc agtcctttac
    21601 gaaaagcagt agagctgcct gctgctatgc ttttacgtaa cgtttcatca agcgatcgtc
    21661 gattcatgta tcctgctaaa aaagtgccta acatggcatc acctgcacat gctgtattga
    21721 caacttttcc ttttggcgag tttcctcgaa tcaccctgtc tttcgtaaaa agaatcgctc
    21781 ctttctcacc gagggataac aaaacatttt ctgcgcctga ctctactagt cgttgtccat
    21841 aaacaaagta atcttcatct gtaaccattt ttcgtccgaa ccaagaagaa agttcttcct
    21901 catttggttt cagcaaaaac ggtcgatagg gtaaacaatc catgacttct tgatcactac
    21961 tatcaataat caaacaaatc cgactgattt ctattaaaac ctttggagac agccctcttg
    22021 gtaaactccc agaaacacaa agataatccc ctgcttgtaa tgctcttatt tgatttaaaa
    22081 aattatgaac agctgtctgc ggtatttcag gtccttgatt cactaatttg tattcttctc
    22141 ccgtttgatt gacttgtgta aatacattga tacgcgtcat accaggcact tcaataaact
    22201 ctgtttggat ttgtttctgc tgtaaatagt cttctatata ctttcctgtg aacccagcag
    22261 aaaaacctaa tgccgtactt gggaaatcta gcatctttaa tactagtgac acattcaccc
    22321 cttttccgtt tgcctgaatg tcatcctctt ttgtgcggtt taccataaaa ggatgcatct
    22381 catctgtttc aataaataga tcaattgcca aattcatcgt gcatgtgtag atagacataa
    22441 ccaacgacct ccttgattct attatgaaat acgcgaaata gaaaatgttt tcttaatcgt
    22501 cccaacacaa agaaaacctt tacatttata aactcgtcgt gcttttttta actaactttt
    22561 ggtgaaagaa ggaaagaata tgaatcacaa attaagtgaa acagagcagt acctctggaa
    22621 ctttatcgaa cacctcatcc tagagatccc taactattcc atcgtgaaac taagtgaaca
    22681 agcaaatgtc tcaacagcca ccattgtgcg gacaatgaaa aagaaaggct atgaagggtt
    22741 tacttcgttt aagcatcatc taaaagaagg agaaaacaag aacatcaatt tttcattttt
    22801 agataaagta gacaaaggaa tccgccgagc cattctaaaa aatgaacaag aagtggtacg
    22861 tacaatcaat atgttggaaa tcgggaacat tgaagatgca atacaaagaa tcaaatttgc
    22921 tgaccgggtt tttgtctttg cccgaggatt ttcagaaatg attggccaag aaatgctggt
    22981 aaaacttcaa ttaacgggaa aaaattgtca gatgcatact gatccagaaa ttatcaaaag
    23041 catcagtcgg aaactaacta agaaagacag cgttattttt gtttctttga acggtgagaa
    23101 aacaaataaa aacatcaaaa ataagattta tgtctttatt tttcaatttt ttaaat
//
LOCUS       DACDTH010000040        23076 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDTH010000040 DACDTH010000000
VERSION     DACDTH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.040"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            102..2543
                     /locus_tag="IWK54_001714"
     CDS             102..2543
                     /locus_tag="IWK54_001714"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9593835.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="IWK54_001715"
     CDS             complement(2873..3577)
                     /locus_tag="IWK54_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593836.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="IWK54_001716"
     CDS             complement(3764..4009)
                     /locus_tag="IWK54_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593837.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="IWK54_001717"
     CDS             complement(4026..4877)
                     /locus_tag="IWK54_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9593838.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="IWK54_001718"
     CDS             5043..6047
                     /locus_tag="IWK54_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9593839.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6288..6644)
                     /locus_tag="IWK54_001719"
     CDS             complement(6288..6644)
                     /locus_tag="IWK54_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9593840.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7012..8199
                     /gene="tuf"
                     /locus_tag="IWK54_001720"
     CDS             7012..8199
                     /gene="tuf"
                     /locus_tag="IWK54_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9593841.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="IWK54_001721"
     CDS             complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="IWK54_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9593842.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..11723)
                     /locus_tag="IWK54_001722"
     CDS             complement(11004..11723)
                     /locus_tag="IWK54_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9593843.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12043..12564
                     /locus_tag="IWK54_001723"
     CDS             12043..12564
                     /locus_tag="IWK54_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9593844.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12623..13483
                     /locus_tag="IWK54_001724"
     CDS             12623..13483
                     /locus_tag="IWK54_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9593845.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13590..15983
                     /locus_tag="IWK54_001725"
     CDS             13590..15983
                     /locus_tag="IWK54_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9593846.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16165..18006
                     /locus_tag="IWK54_001726"
     CDS             16165..18006
                     /locus_tag="IWK54_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9593847.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18030..19139
                     /gene="rpoD"
                     /locus_tag="IWK54_001727"
     CDS             18030..19139
                     /gene="rpoD"
                     /locus_tag="IWK54_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9593848.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19359..21458
                     /locus_tag="IWK54_001728"
     CDS             19359..21458
                     /locus_tag="IWK54_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9593849.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21654..22517
                     /locus_tag="IWK54_001729"
     CDS             21654..22517
                     /locus_tag="IWK54_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9593850.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22622..>23076
                     /locus_tag="IWK54_001730"
     CDS             22622..>23076
                     /locus_tag="IWK54_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9593851.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         7770 a         4038 c         4810 g         6458 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
     6181 ctaaacacgt ataaacggcg gaagcagaag caactccttc ttatttcttg gagttaaacg
     6241 cttctgtccc agcctctttt tatctaataa aattttaaga ccatttacta attatcgatt
     6301 ttttctgccg catttttagg cacttcagaa gcctttattt cttcccagct taatacacct
     6361 ttacgaggat tgactttgag tttaagatac gcattcatct ttaatggatg ttctctttct
     6421 tcgcgaagcg tcacctgttt tttttcacca tctttattat aagccgcttg cttgtaggta
     6481 taagtcgtga atttctctcc gttatctgct tgggtagctg acatttcgcc attcgtcgtg
     6541 atttttgtat aataagcttc gccgccgtaa aaataatgat aagctgcaaa acttccgccg
     6601 agaaatatgc ctaaaacgac aagtacgcca attaattttt tcatgtactt cacctctttc
     6661 cttattatta acgaaataga agatttcgtc tattgaaagt tgttggcaac agctgaattt
     6721 taaacttttc ctcattttgt tcttattgct tcaaaagaaa gttaaggact atcttgaagt
     6781 ttttctcaaa aaacacattt ttttatttat aaaaggtact tttttcgcgt ataagacaga
     6841 cgagtgctct tagttgactt tgaccaatat tgacgtataa taagcttagt tcgaatctta
     6901 caaaaagtaa gtcggacgta ttctttttga tttagtcaaa aaaagactat aataaaaagg
     6961 tgagagctga aatttttcag tgatctatat tatttaggag gtttgtaaat tatggcaaaa
     7021 gaacattatg acagaagtaa accacacgtt aacattggta cgattggaca cgttgaccat
     7081 gggaaaacta ctttaactgc tgcgatcact acagtattag gcaaaaaagg cctagcaaat
     7141 cctcaagact atgctagtat tgatgcagct ccagaagaac gtgaacgtgg gattacgatc
     7201 aataccgcac acgtagaata tgaaacagaa aaacgccact acgcgcatat tgatgcacca
     7261 ggacacgcgg actatgtgaa aaacatgatt acaggggcag cacaaatgga tggtgcaatc
     7321 ttagttgttt ctgcaactga cggtccgatg cctcaaacac gtgaacacat tttattgtca
     7381 cgccaagttg gtgtaaaata cctgattgtt ttcttgaaca aagttgattt agtcgatgat
     7441 gaagaattga tcgatttggt agaaatggaa gttcgcgagt tattgagcga atatggtttc
     7501 ccaggcgatg acactcctgt gatcaaaggt tccgcattaa aagcattgca aggcgatcca
     7561 gatgctgaag ctgctattat ggaattgatg gatacagtag atgaatatat cccaacacca
     7621 gaacgtgata cagataaacc attactattg ccagtggaag acgtcttctc aattacaggt
     7681 cgaggaactg ttgcctcagg tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta
     7741 gagatcgtag ggatcaaacc tgaaacacaa aaagcagttg taacaggtgt agaaatgttc
     7801 cgtaaaacgt tagattacgg ggaagctggg gataacgtag gcgtgttgtt acgggggatc
     7861 caacgtgacg atatcgaacg tggacaagta cttgctaaac caggttccat tactccacat
     7921 acaaaattca aagcagaagt gtacgtgttg acaaaagaag aaggtggacg tcatactcca
     7981 ttcttcaaca actatcgtcc acagttctac ttccgcacaa ctgatgttac aggaacaatc
     8041 acattgccag aagatacaga aatggtcatg cctggtgaca acgtgacgat tgatgttgac
     8101 ttgatccatc caatcgctgt tgaaaacgga acgacattct caattcgtga aggcggacgg
     8161 acagtaggtt caggtatcgt tacagagatc gaagcataag aaacgcgact cgaatatttt
     8221 ggtaatgaaa aatgtttaaa ataaaagagc ggtccaaaca ggatttctgt ttggaccgct
     8281 ctttgttctt caattgtata agaagagatt gtagaagcat gaactttcgt tttacttcag
     8341 tctcttttat tcatcatgag agtgttcatc tatcggtatg cagtttttaa tactttcttc
     8401 gtatccattt tttcaaacca cttattctac cttgtgtctg ctcaactggc gcattatcaa
     8461 taatcagcaa agcaatcata acatatgcaa tccaactatc tctagaatat agagtataac
     8521 gtgagaccca gtttgtagcg tacttgcttt tatattctct taatccatgg aaagaataaa
     8581 aatgagaacc aaactcgtaa accaagtatg caatcctttc ctgtaaaaaa ctctttctgg
     8641 aagttccgac atttgaaaga ggggccatcc ctaaattgaa ccattggata tgttcttctt
     8701 tcatataggc aaacaaatgc aagaacaaga aatccatact gccagaaggt gcttttttag
     8761 aatcatatcg catcagatca atcgttccta ctttattgtt cgtgtaagta ggcatgatcg
     8821 ttgcaaaagc gacaatttct tcttgggtat ttttgacaac tgcaataggc gctctttgta
     8881 gataatcttc agagaaaaag cctaaagaaa accctttttc ctttcgtgaa cccaaccaat
     8941 tatcagagat atttttgaaa gtactcattt gctcagtaga aaatggtggt tgtaagacat
     9001 caaatgtaaa tccttctcgt tcgatccgat tcaagacagc acgagtgccc ttcatttttt
     9061 tccctgaagt tgtaaaacta ttcaggtcta cataagcttc ttcccccatc ttaatgaaat
     9121 cgtagccgaa ttcatgcaaa atcataacaa tctcttcgct tgtttcgtag aaaactggta
     9181 aatagcatag tcgatctgtt tcttcgataa aagcttcgat tgcttctgga aaatcttctt
     9241 tcttaccaga aggatcaccc attacaatac atttattatt gaagcaagcg aattggagaa
     9301 gaacagttgg ttcaccgtct ttttcatagg caaacatccg cttatctttt aagaagatca
     9361 attgactatc ggagtttcct ccataagttg taaggatctt cagggccttt tcttcattaa
     9421 aggcttcgcc aatctgtttc ttttctcctt gaaggaaatg aacaaataga acgatcatga
     9481 aactaacagc gatgatggcc aacaatcccg aaaaccaaat cttttcagaa ggaaataaga
     9541 aaaaggaaat aaaatggtga cgccgatgag ggaaatccgg cagattgtat accccaataa
     9601 cgatgtaaag caaggacagt gcaccaataa tgattccatc gatcgtacgc cactcccatg
     9661 aatagatcaa ttgttcccga aataattcgt ttttagaagc aagaatgatc agaagcagaa
     9721 tagacaagaa aaccaccggt gtgaaactaa aatcacttaa taaaacataa agtaaagcta
     9781 gaacaatcaa gaagattgta ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc
     9841 ccataatgat taaaagaaat cccaatagta tactaggaaa ttggataata aaatgaaatt
     9901 tcaatgggtt caaactatga agccagcgaa actctgtaaa agcttgcggg atagtggcag
     9961 aaagtactag catgattcca gaaaagtata ataagacaac cacgatttta tgcgcaattt
    10021 ctgttgctaa ttgtttaggg ataccagaat aacgctgatc aaaagtggaa ccgatatttt
    10081 tgaagaaaaa gacgattcca atcaaaaacg gaatgatgta gtagaacagg cgatagagca
    10141 agatccacac aacgactatt tctcttggca caccaatagc ggacaacccg ataatcatca
    10201 ttacatcgaa actcccaatt tctcctggaa tcatagacac gattcctatc actgaagcag
    10261 caatgaataa tggaattgtt tgccacagat caatccggat ttccattaaa taaccaacag
    10321 aaaggaaact aaacaggaca ccggtccatt ccaacaatga aactagcaat agctgtcctt
    10381 gagttttaag tgaggtacca ccaaggaatc ctttcttttt gaaagtagta aataaaaaga
    10441 cgacaggaaa atatagaccg ccgccgatca accatatcca atattgcttc aaaaaaggag
    10501 caactggcgt gaagactaca agtaaaaaag aaagcagact gtaaatagaa agtccagcca
    10561 tcaaaaacaa gaaaactttc gataatgctt ggatcacgtt tctgctctct ttcttttgtc
    10621 catatagttc agagcgaagc cccacactaa caaacccgcc gaaaccggca atattgttga
    10681 tcgtattgat cagccagctt gtctcaaaca ggtaacgagg attctgcttt tgttcaagca
    10741 tccgattcaa aataatatca taaccaacca taggtaacac agagataaga ccaatcaata
    10801 gcattagtcc gcttttccat acaggtatag tggcaaacgt ttctcctaat tgctggatag
    10861 atagagtttt accgatggac atcaattcac cgacaataat gacaatgaca gaaatcaaaa
    10921 agattgtttt gaacagtcct aggtggtttt tcagccattg tatggtattt tttaacaaat
    10981 aaatcacctt ctattcctaa tagttatgtt tcagaaagaa attgaagaac ttctcgattg
    11041 aagcgttcag gatcttttct agctaattca tgtccttgtt ctttcacaag cacaaaggaa
    11101 gcttttggta tagttttagc tatatacaaa gaatgtttta gcttgattac atcttttttt
    11161 cccacgatga tcaaagtggg ggagttgatt ttttttaaat cattttctgt aagcccgatg
    11221 tcatgcagca aaagtttgat aactaaaaga tttttgcgaa gacttgggcg aaataaagag
    11281 agtaaccata cccatgcata atgaatatta ctgatgactc tggcagaaaa acgaacgcct
    11341 ttaacaagcg tgtttccaga atttaaaatc aatcgatgaa cttttttagg gaagctgctg
    11401 gcaaatacca gtgctagatt tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt
    11461 ttttccagca acatgatcgt attcagatcc tcggccatta actgaaaatt caacatagat
    11521 gcttcgtttg tcgaacgacc atgtcctcgg ctatctacta gatacacggt atagtagcgt
    11581 tcgagtacag ggacctgttc tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa
    11641 aagagaggga agccttgacc gctcttttca tagtaaattt cgctcccatc tggcattttt
    11701 gcatatttct tttctttttt cacataatca cctactgtgt aaagtataac gattatatca
    11761 gtcattgggc aattgttttt actgaaaaac aacgatcatt ttcgtaagat gtcaaaaaat
    11821 acaggaaaat tcattttttc ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa
    11881 gataaagaaa tagtaagata aaaaaagatg tcaaaataac taggtgatga aatgaagcga
    11941 aaacgattat ctattatccg tattcttttt tacatgatca ttctgacggt tattggtgcg
    12001 gtgttgtttt tatgaggaag aacttatcgt tcggtaaaac gagtggaaag ctatcaaata
    12061 gaaataacaa cagctgcaaa aaattatcat atggaaaact acgagaattt gatcaaggcg
    12121 ataatattga ccgaatcaaa aggaaaaggg acagatctca tgcagagctc ggaaagtctg
    12181 catggagaag ctgagatcat tgataatcca gctgacagta ttgatcaagg tgtcaaatat
    12241 ttggcggaaa tgatagtaga agcaaagaaa caaggatgcg atttatggac agctgtccaa
    12301 gcatataatt ttgggaaaga ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca
    12361 gtagaattag cagaagcgta ttctaaagat gtgctgagcc cacttttagg aaatgaggaa
    12421 gggaaacaat accgatattg gcggatccag tccttgcttt acaatggcgg ctatctctat
    12481 cataatggag gtaacatgtt ttatcctgat attgtcaaaa tgaatgaaaa aataatagag
    12541 acctatgaaa aagtctttaa gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa
    12601 tgaaagaagg agtgcgcaaa tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg
    12661 gtacacaacc aaagactgga tgttacggtt tgtgaaggga atgtttatcg gttcaggatt
    12721 tatcttgcca ggagtcagcg gtggtgcatt agcagctgtc tttggcatgt atgagcggct
    12781 gatttctttt ttagcccacc ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc
    12841 tgttggtcta ggcggagtga caggcatctt tctactttct tttttagtca gttttttatt
    12901 aggtgcgtat gaaacaacga tattatggtt ttttgtcgga tgtatcgctg gaactgttcc
    12961 tgcgttatgg aaagaagcag ggaaaaaagg aagagatact tctgatgtga tcatcatggt
    13021 tgttactttt gttctggctt atttgttttt attatacggc gctcaattat ttgacgggca
    13081 agtcgatcag aattttggaa catggctgat tgccggcgga ttgattggtt tgggtatgat
    13141 cgtcccagga ttgagccctt ctaatttttt ggtgtatatg ggattgtata aagcgatggc
    13201 tgacggattt aaagaagtac gacttgaagt gattgtgccg atcggaattg gtgggattct
    13261 ttgtgttctt ggattgtcaa aaatcatgga ttacattttc tcaaaggcat atagcaagct
    13321 attccatttc attttaggtg tagtttttgc ttcaacgatt atgatcattc ctaccgatta
    13381 cgcgggattg ggagttgctg gggtactagc ttgtcctgtt ctttttatag ccggtacagc
    13441 actaggttgg tggatgagcc gtttggagga acaatataag taaagataga ttgataataa
    13501 aaacagcaca cgggtgattc aaacaaaaag gttggcatct tttttgtaaa atcattcgtg
    13561 tgtttttcta taaattttat aaaaacaagt tgtttttaat cttggaacac gctagactta
    13621 aacacgtgat tatttttgaa agttggcgga aaatggagaa aaagaaaatg aatggactta
    13681 cacagcaaga ggtagttcag aaaatggctt ctggccagca aaatgactac caggaagatg
    13741 catcaaaaag tacaaaacaa atattcagcg ataatatatt gacgttattc aattttctga
    13801 actttggtat tgggatttgt ctgttatttg taggtgcgta ttccaacatg gcgtatttag
    13861 caattattgt cgtcaatatc gttattggaa tttatcaaga aatacatgca agaaatcttg
    13921 tacggaaatt atcgattgtt tcccaatcag aagtactagt gatccgggac ggtaaagaac
    13981 aatcgatttc tactaaagag ctggtattag gagatttggt aaaaatcgga gcaggagaac
    14041 agataccagc cgatttacag gttatatctg gacgggcaga agcaaatgaa gctcttttga
    14101 caggcgaatc tgatttgatt gtcaaagaaa ctggagctga attgctttct ggaagctttt
    14161 tgacaagcgg ttctgtcatg gctgaagtta tccgagtagg tgcagataat tatgctgtcc
    14221 gcttgactag cgaggcaaag actcacaaag ctattcgttc tgaacttgta gattcgattc
    14281 gcaaagtatc caaattcact agtttcgtga ttattccttt ggggatcata ctatttttgg
    14341 aagcactgtt catccgcgat gcttctatca aaatttctgt tattgcatct gcagcagctt
    14401 tgttaggcat gcttccaaag ggtttggtat tgctaataag tattgcatta acgacagggg
    14461 taaccaaact ggcaaaaaaa cgaatccttg ttcaagacat gtattcaatt gaaacgctgg
    14521 cccatgtcga tacattatgc ttggataaaa cagggaccat tactgaaggg aaaatgagta
    14581 tccagcgggt agaagcacta catccgctgt acgagaaagc catcccagaa attatgggga
    14641 cgtatctggc agaaagcagt gataacaaca tcactatgcg ggctatgaga gattattttg
    14701 agctttctaa tcggtatgat gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg
    14761 gcgcgatgga atttccagaa ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg
    14821 aagaagatcg gcttccggac agtctaaaaa cagcccaagc gaatggctat cgcgtactta
    14881 tgttggggat cgctccaaat cagcaattga acgaagaaaa accagcattt gttgagccgg
    14941 ttgctctgtt tgaaatcgat gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc
    15001 ttcatgagga aggtgtggat ctgaaagtga tttctggaga caatcctttt actgtctcca
    15061 atatcgctag acgtgcagga ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta
    15121 ctgaagaaat ggaagtgcga gaagctgctc atcgctatac ggtttttgga cgagtgagtc
    15181 cgcagcaaaa gaaattgctg gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga
    15241 caggcgacgg tgtgaatgat gttttggcgt taagagaagc agattgcagc attgctatgg
    15301 cagaaggtga tggagcaaca cgtcagattg cgaatctggt attgttggac tctgatttta
    15361 ctacattgcc agaagtcctg tttgaaggac gccgagtggt caacaatgtg acgaaagtct
    15421 caggcatctt ttttatcaaa acgatttatt catttatttt atcgattata tgtgccgtta
    15481 cagcaattgg atttccgttt atccctatcc aaatcacgct gattgattta gcgattgaag
    15541 ggtatccttc cttcttttta tcttttgaac aagataaaag aaaaatcact taccgttatt
    15601 tgcctacagc acttattaat gctttaccaa atgccttgct tgtagtagtg aatatcattg
    15661 cggtgtatct acttggaaaa acattcgcat ggggaaatgc tgaaactact actttgatgt
    15721 attacttgct gattgggatc agttgcatgg cagttgtaaa agcttgttat cccttcaacc
    15781 cattgcgcat cttcctagca gtaacgacga tcgtagggat atatgtagct gcaatgctgt
    15841 tccatggaat tttggaagta aacttagggt tgggagagag cgtaggatac ttctttgtat
    15901 tcatgctcgt aaatattgtt cttcgagtat tgtactggaa ggctgatatt caaaatcagt
    15961 tactgaaaag agtagcaaat tgatgcttaa tgagatagaa atgtgttata cttgaaaaag
    16021 caatgaaata tgatacaatc aaacatgtat aattgtgtcc tttaaaatcg cactgatttc
    16081 agttagtgcg attttttacc ggttattctc ctgaaatatc ttctgttaaa cccaaaagat
    16141 atcaatagag cagggggtga aaaaatggct caacggattc ctcaagaggt tatcgaaaca
    16201 gtacgcagtc aaacgaatat cgttgaggtg attggacaat atgtccagtt gaaaaagtct
    16261 ggtaaaaatt acttggggct gtgtcctttt catgaagaac gtacaccgtc tttttctgtt
    16321 gcagaagaca agcagatttt tcattgcttt ggttgtggta aaggcggaaa cgtgtttact
    16381 tttcttcaag aactagaagg tctaagtttt ccagaagcag tggtaaaagt ggcagacttc
    16441 gaacagatac cgttagaaga tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa
    16501 tctgctacgg ggaaactgat tgctgctcat gaaaaagcag cggaaatcta tcatcacatg
    16561 ctattgcata caaaagctgg acagccagct ttagaatatt tgcaaagtcg tgggttgtct
    16621 ttagaattaa ttgaagaatt caacataggt tttgcaccta atgagcgttc ttttcttcag
    16681 caagtattca agaatgaatc aatttcaaat gaattgttag aacgctctgg attgtttgtc
    16741 acgcatgatg atggccgtat gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt
    16801 aattctcaag gccagacgat cggtttttca ggacgctggc ttgcacctga aaaagggcag
    16861 gaaaaagatc agcccaaata cttgaatagt ccagaaacag aactgttcaa taaaagacaa
    16921 gttttattta atcttgataa agcaagaagc gatatccgga aaaacggaga aattttgttg
    16981 ttcgaagggt ttatggatgt gattgctgca tggcaggctg gtatcaaaac tggattagct
    17041 tctatgggga caagtctaac agctcaacag atccaacaaa tggaaaagct gacgaaagaa
    17101 ttggtctttt gctatgacgg agacaacgca ggttttgaag caaccaatcg agggattgaa
    17161 ttgcttcggc aaaacagtcg attagcactt tctgtcgtag tcgttccaga aaagcttgat
    17221 cctgatgaat atctccgtaa gtatggagaa gcatctttcc aagaattagt ggctcatggg
    17281 cgcgaaacag tctttacttt taaatcacgt tatttaaaac aagggaaaaa tttagataat
    17341 gaaaaagaca agatcgaata cctcgaagaa ttactagttg aacttagccg agttgtttca
    17401 ccaatcgaac aagatatgta tctatctcaa ctgtcaacag agtttcagat cggcagagag
    17461 acgatacaaa aacaactgcg cgatcttcga aggacaaatc agcaaaatcg tcaagaacct
    17521 caggaaagtc aacacactca ggttacgaga caagaaactg cacggaaaaa gagacctttg
    17581 acgcaagtag aaaaggcaga gcagattcta ttatatcgtg catttaaaga agtttctgtc
    17641 cgtaatattt taaaagaacg agaatttcag tttattcatg atgcatatgc agaagtttat
    17701 tttttattcg atgcatatgt ctcacaacat tatgaattca accttgcaga gtttttagac
    17761 tttttgcaag atgaaaattt aaggcggttg gtggtggaaa tcgaatattt accggtggca
    17821 gaagaaagtt cgccacgaga aatccaagac ttgctagatg tgatcagcaa atctggctta
    17881 gctgatgaaa taacattaaa aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa
    17941 cagcttgagc ttgaattgac gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc
    18001 aaatagctac tcaagggggg ccttctttta tggcaaatgg aacagataaa aaaacgtatg
    18061 aagcagcagt agcagctttc atcaaagaaa acaaacccaa acgacaagtt gtatatgatg
    18121 atttatccaa tcagcttgct acaccattca ctttgaatgc agatgaaatg gacaaattga
    18181 tccaaaagat tgaagacgca ggtatcagtg tggtggatga aaatggtgat ccatcaattc
    18241 acagcttgaa gagtgctgag aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc
    18301 caaccggtgt aaaaatcaat gacccggttc gtatgtatct gaaagaaatt ggccgtgtct
    18361 cccttttaac tgcggaagaa gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag
    18421 aagcgaaaca gcgtctggca gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt
    18481 atgtaggacg aggcatgcag ttccttgact tgatccaaga aggaaacatg ggactgatga
    18541 aggcagttga aaaatttgac taccgcaaag gatttaagtt ttcaacctat gctacatggt
    18601 ggattcgtca agccatcact cgagcaatcg ctgaccaagc acgtacgatc cgtattccag
    18661 ttcatatggt tgaaacaatt aataaattga tacgtatcca acgtcaattg ctgcaagacc
    18721 ttggaagaga acctacgcca gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag
    18781 tccgagaaat cttgaaaatc gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag
    18841 aagatgactc tcacttaggt gatttcatag aagaccagga agcaacaagt ccagcagaac
    18901 acgcagctta tgagttacta aaagaacaat tggaagatgt actagataca ttgactgatc
    18961 gggaagagaa tgtccttcgc ttacgttttg gtttagatga tggccggacg agaacattgg
    19021 aagaagtagg gaaagtattt ggtgttactc gggaacgtat tcgtcagatc gaggctaaag
    19081 cattaagaaa attgcgccat ccttctcgtt caaaacaatt aaaagatttc ttagaataag
    19141 cgtggactat tgaccagaag agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa
    19201 aagcttgaga ctacgcagtc tcaagctttt ttttctatag gaaggaaatt cattcattat
    19261 ttttaagtaa atcctaaaaa taagctattg gcgtgacgta tagaatatga tagattaaat
    19321 ggtagcgtaa acctagtaaa ggagccgatc gctaagtgat gaaaaaatgt ccaaattgcg
    19381 gatcgaaaaa aatggagcag aatactatct gtccagactg cggtcatcaa ataactgcag
    19441 aagatcagca aataaaagaa tcaattgaag aaacaaatac agatgcagct atgcaggata
    19501 aatcaactga agatgcagtc tttcctgata cagaattaaa cgatccaatc gaatggtcgg
    19561 aattgaaaga tttacctcta gaatctgtaa tggaattatt cgaaaaagca gaaccagaag
    19621 acgaagaacc acctgctcac gatcaagtaa ctaatataga gaaaaatgaa cagaagaaag
    19681 caactgttca tgagacttcg gaagaatcag gaaagaatca agaagaaacg cttgaatcaa
    19741 aacaaaaaag aagagtagct gaactgaaag aaatcgtcga taatgaggaa gaaaattcga
    19801 tactatccgc ttatatcaaa gcacatcgtg aagatacaaa agaagagcac gcgaaagagc
    19861 tgctcaaaat gatcagtgaa aaaattgcac aggaaaacaa agtagagcca gaaaaggaac
    19921 aagaaactga aaaagactct gatagagaaa acgccacagg taaagaaaca gtaaaaataa
    19981 aagacgtaaa aacggatatc acagaaaaaa ctgaagtatt tactgaaaaa caattagaaa
    20041 aggaaaaaac gaataaactg ccggaagcag agaaaaaaga aatactcgaa aacacagaca
    20101 aaaagtcaac agagcccgat gaaataaata gtgaatccga tgctgaaaat caatctgtaa
    20161 gcaaagaggg tactgatctt gaagcaaaaa tagaaaaaga agagaaccag cctaagctgt
    20221 ttgaagcaga agcgaaatca ataacaatcg tgccagatac tgaaaaaaca catcaagaat
    20281 caaacttaga aaaaacagat ccagataaag cgcaaaagaa aaaatcaaaa aaagtacctt
    20341 atctcgtttt agcagcagta cttctactcg gtggaggaag ctgggcttat tatgaccatc
    20401 atcaaaaagt agaagccgaa gtagcagcag aagcaaaaag acagcaacaa atggaagaac
    20461 ttcatgagaa acttgctgcc ttttatacag atgatcacca tcaatttatt cgtgcagata
    20521 tgattcatca agatttatca aaactaaaaa atgatttatc cagtcttaag aatgaaaaag
    20581 gatattcaga gttagaaaca acttaccaag atatccaatc taagatccga gatatccaaa
    20641 aagtgaatga gtggttcata gcacctgcca tcgtcgatga tcatctagca gatgacatca
    20701 agttgaaagc agataaagag atccaagcaa tcgaaacgga taacacagat tttggaaaat
    20761 tgattcaaca agcacaaaca gaagctaaaa atcaatatgc tgaattgcaa acagctaaag
    20821 aaaaaacagc tatagtcttt aaagacggaa aagtagtgga ttcagcaaca cgcgaccaat
    20881 acaagacggc aaaagaagca gtagaaaaag tgaaaaatac agatctatct gctcagttga
    20941 atgaacaatt gaaacaagta gataagatgc tcactgataa agagaaaaaa gaagaagagg
    21001 aaaagaaaaa agcagaagaa gcgaaaaaag cagctgaaga acaagcaaaa caagccgctc
    21061 aagcagcagc tgcagctcaa gccgctcaac aagctgcggt cgcacaagca gcacagcaag
    21121 aagcggcatc ctcgacccct tcctcttcaa actcagcgaa tcagccaatc aaatctgcca
    21181 gagcaagtga tattgccgat gcatctaatc cagcatggac gtgggcacca ggtgtccaaa
    21241 gcaaagtgat tgctacatgt atccaacgtg gatatatcgt tgaaggaggt tacagattag
    21301 aaaaagccag aatcgaaaat ggggaaggct attataatct atatgccact tcaacaaaat
    21361 ctgcattgat gaaaggcatt ggagaaagtg ctttgccatt ttacatcgta accatcaact
    21421 gtaaaactgg atggttcgga ggaaatggga gcaattaatt ttaggcaaat gaaagaggct
    21481 gtgagcctgc cgtttaacac cgtttattcg aaaaagggga gtatgacaca actgttgtca
    21541 tactcctttt ttcacctttc caaaccaagg ctcagctatt caagtagata acattatgtt
    21601 atgatagcaa agaaatgtat taagaaaacc tttcactata gagggagaaa aaaatgaacg
    21661 atttattagc aactatattt acaggtatta ttattgatga aaatgaaaaa aactactttg
    21721 tccaaaaaaa cggacaaacc tttggcttaa gcaaatcaga aggtgaacac aaaatcggtg
    21781 aagcagttga aggattcggt taccaaaatc aaaaacatga aaatcggatc actacacaaa
    21841 ttcctaaaag ccgtatcggt cattacgctt ttggaatcgt tactgctaca cgaaaagatt
    21901 taggtgcgtt tgtcgatatc ggtcttccag ataaagactt tgtcgtatct cttgatgaat
    21961 tgccgacaat gacagagcta tggcctaaaa aaggggatcg cttgatgatt tcccttagag
    22021 ttgatgcaaa agaccgtatt tggggcagtt tagcagatga aaaaatattc aaatctttaa
    22081 gcaaatatgg aacagaagaa atgaaaaaca aagatgtgat aggaacagca taccgcttga
    22141 aattaacagg tacgtacgtt ttaacggaag atttctatct tgggtttatc catccttctg
    22201 aacgatataa tgaacctcgt ctaggcgagc agataaaagg tcgagtgatt ggtgtccgac
    22261 cagacggtgt actgaatctt tcattgaagc caagagcata tgaagctata tcagatgatg
    22321 cacaaatgat tttaaccatt ttagaacgtt ctgcagatca gcaaatcaac tttacagata
    22381 aatcggatcc agaagaaatc atgaaagctt ttggtatcag caaaggagca tttaagcgcg
    22441 cattaggtaa tcttttgaaa caaggattga ttacacaaga aaacggtgtg acaaaactag
    22501 tcaaaaaatc taattgagaa tcccttgcat gtttttctta actagcttat aataatttta
    22561 attaacatag tgttatatct ttttttattt gtaattatta taaattgttg gggttgagta
    22621 aatggtcatg aatgctattt cggcgatgaa aaaaacaaaa cagcagctac atgaagcggg
    22681 gtttaagctg acaccgcaaa gagaagctac tctgcttgtg cttttggaaa atgagaaaga
    22741 gcatttatct gcagaagaaa tcttcttttt agtgaaaaaa aagagtccgg aaattggact
    22801 tgctactgtt tatcgaaccc ttgaaatctt gacagaattg aaaattgtcg ataaagtaag
    22861 ttttaatgat ggattagccc gttatgattt gagaaaagaa ggcgccaaac atttccatca
    22921 tcatttacta tgcttgaact gcggcacgat cgaggaagta gaagaggatc ttttacttga
    22981 agtggagcaa gtagtagaag aacgttatca ttttttagta aaagatcatc ggctaacgtt
    23041 ccatggaatc tgtcaggaat gctatgaaaa agaaaa
//
LOCUS       DACDTH010000041        23067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDTH010000041 DACDTH010000000
VERSION     DACDTH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.041"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(111..566)
                     /locus_tag="IWK54_001731"
     CDS             complement(111..566)
                     /locus_tag="IWK54_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593852.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="IWK54_001732"
     CDS             complement(720..1928)
                     /locus_tag="IWK54_001732"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9593853.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="IWK54_001733"
     CDS             complement(2040..2372)
                     /locus_tag="IWK54_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9593854.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="IWK54_001734"
     CDS             2520..3395
                     /locus_tag="IWK54_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9593855.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="IWK54_001735"
     CDS             complement(3477..4058)
                     /locus_tag="IWK54_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9593856.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="IWK54_001736"
     CDS             complement(4064..5323)
                     /locus_tag="IWK54_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9593857.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="IWK54_001737"
     CDS             complement(5325..6371)
                     /locus_tag="IWK54_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593858.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="IWK54_001738"
     CDS             complement(6606..6881)
                     /locus_tag="IWK54_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9593859.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /locus_tag="IWK54_001739"
     CDS             complement(7124..8434)
                     /locus_tag="IWK54_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9593860.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="IWK54_001740"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="IWK54_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9593861.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /locus_tag="IWK54_001741"
     CDS             complement(9980..10660)
                     /locus_tag="IWK54_001741"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9593862.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="IWK54_001742"
     CDS             complement(10709..11344)
                     /locus_tag="IWK54_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9593863.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="IWK54_001743"
     CDS             complement(11410..12813)
                     /locus_tag="IWK54_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9593864.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="IWK54_001744"
     CDS             complement(12800..13831)
                     /locus_tag="IWK54_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593865.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="IWK54_001745"
     CDS             13862..14083
                     /locus_tag="IWK54_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9593866.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="IWK54_001746"
     CDS             complement(14248..14985)
                     /locus_tag="IWK54_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9593867.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="IWK54_001747"
     CDS             complement(15138..16478)
                     /locus_tag="IWK54_001747"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9593868.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="IWK54_001748"
     CDS             complement(16715..18238)
                     /locus_tag="IWK54_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9593869.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="IWK54_001749"
     CDS             complement(18616..19227)
                     /locus_tag="IWK54_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9593870.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19557..20273)
                     /locus_tag="IWK54_001750"
     CDS             complement(19557..20273)
                     /locus_tag="IWK54_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9593871.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="IWK54_001751"
     CDS             complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="IWK54_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9593872.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20832..21629)
                     /locus_tag="IWK54_001752"
     CDS             complement(20832..21629)
                     /locus_tag="IWK54_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9593873.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21599..21970)
                     /locus_tag="IWK54_001753"
     CDS             complement(21599..21970)
                     /locus_tag="IWK54_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9593874.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="IWK54_001754"
     CDS             complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="IWK54_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9593875.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6470 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagt tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
    19501 cattatacat atctattcaa aaaacgcaac atctaacaat tataaaaagt aagtgttcat
    19561 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
    19621 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
    19681 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
    19741 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
    19801 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
    19861 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
    19921 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
    19981 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
    20041 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
    20101 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
    20161 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
    20221 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
    20281 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
    20341 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
    20401 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
    20461 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
    20521 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
    20581 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
    20641 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
    20701 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
    20761 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
    20821 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
    20881 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
    20941 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
    21001 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
    21061 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
    21121 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
    21181 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
    21241 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
    21301 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gccgaattct
    21361 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
    21421 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
    21481 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
    21541 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
    21601 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
    21661 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
    21721 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
    21781 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
    21841 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
    21901 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
    21961 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
    22021 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
    22081 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
    22141 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
    22201 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
    22261 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
    22321 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
    22381 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
    22441 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
    22501 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
    22561 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
    22621 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
    22681 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
    22741 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
    22801 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
    22861 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
    22921 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
    22981 ctggtttctt ttttcggctc tataatttat gggactgatg aatcagaatt gattcttcag
    23041 ccccattttc attttaggta ttaaaaa
//
LOCUS       DACDTH010000042        22688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDTH010000042 DACDTH010000000
VERSION     DACDTH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.042"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..652
                     /locus_tag="IWK54_001755"
     CDS             <1..652
                     /locus_tag="IWK54_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP9593876.1"
                     /translation="GMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIH
                     DRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETAL
                     NQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGIL
                     RRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(723..2033)
                     /locus_tag="IWK54_001756"
     CDS             complement(723..2033)
                     /locus_tag="IWK54_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9593877.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(2045..3463)
                     /locus_tag="IWK54_001757"
     CDS             complement(2045..3463)
                     /locus_tag="IWK54_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9593878.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3703..5271)
                     /locus_tag="IWK54_001758"
     CDS             complement(3703..5271)
                     /locus_tag="IWK54_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9593879.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5275..5556)
                     /locus_tag="IWK54_001759"
     CDS             complement(5275..5556)
                     /locus_tag="IWK54_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9593880.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(5553..6476)
                     /locus_tag="IWK54_001760"
     CDS             complement(5553..6476)
                     /locus_tag="IWK54_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9593881.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6473..7351)
                     /locus_tag="IWK54_001761"
     CDS             complement(6473..7351)
                     /locus_tag="IWK54_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9593882.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7338..8675)
                     /locus_tag="IWK54_001762"
     CDS             complement(7338..8675)
                     /locus_tag="IWK54_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9593883.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8678..9781)
                     /locus_tag="IWK54_001763"
     CDS             complement(8678..9781)
                     /locus_tag="IWK54_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9593884.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9855..11363)
                     /locus_tag="IWK54_001764"
     CDS             complement(9855..11363)
                     /locus_tag="IWK54_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9593885.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11329..12021)
                     /locus_tag="IWK54_001765"
     CDS             complement(11329..12021)
                     /locus_tag="IWK54_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9593886.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(12043..13017)
                     /locus_tag="IWK54_001766"
     CDS             complement(12043..13017)
                     /locus_tag="IWK54_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9593887.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13317..14189
                     /locus_tag="IWK54_001767"
     CDS             13317..14189
                     /locus_tag="IWK54_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9593888.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14771..16969)
                     /locus_tag="IWK54_001768"
     CDS             complement(14771..16969)
                     /locus_tag="IWK54_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9593889.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            17201..18193
                     /locus_tag="IWK54_001769"
     CDS             17201..18193
                     /locus_tag="IWK54_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9593890.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            18253..19674
                     /locus_tag="IWK54_001770"
     CDS             18253..19674
                     /locus_tag="IWK54_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9593891.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19667..21166
                     /locus_tag="IWK54_001771"
     CDS             19667..21166
                     /locus_tag="IWK54_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9593892.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            21205..22599
                     /gene="uxaC"
                     /locus_tag="IWK54_001772"
     CDS             21205..22599
                     /gene="uxaC"
                     /locus_tag="IWK54_001772"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9593893.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6896 a         4619 c         3934 g         7239 t
ORIGIN      
        1 cggtatgaga acactttatc gtttattttc taaaggaata tttgatattg acacactacc
       61 gatgaaaggt aaaagaaaac ccaatggcca tcaggaaaaa cggggaaaac aacagtatca
      121 gcgctcaatc catgatcgac ctgataatta tcctgatttc aattctgagt ttggtcacct
      181 tgaaggtgat acgatcgttg gcattcatca taaaagtgcc gtcattactt tagttgaaag
      241 attatctaaa gtcattatca cgattaaacc caacggccgt aaggcattag atattgaaac
      301 tgcccttaat caatggtttt ctcgcttccc taaaaacttc tttaaatcta ttacgtttga
      361 ctgtggaaaa gaattttcta actggaaagc cattagtaac caacatgata ttgatatata
      421 ttttgcggac cctggaacac cttctcaacg cccattaaac gagaattcta acgggattct
      481 gcgtcgtaat ggactgccga aatcaatgga ttttagaaaa gtgaatcaga catttatttc
      541 cagtgtcagc aatcaacgta atcatattcc aagaaaatca ttgaattaca gaacaccaat
      601 cgaaatattt ttgagctatg tacaagaagc attttattct agcttaattt gacaaatcat
      661 atatcattaa aaaaaataaa ggtcaagcta aaatattatt taagcttgac cttattataa
      721 tttcaacgat tgtcaaaagt attgtttgca gctgtgttgc cagtttctat agataaatca
      781 cagcttttga aacataagtt ttgtactgct tcgattcgcc cgaattcttt tgcagttatt
      841 tggcaatgag aaaagcagac atcttctata ggcttttcag gaaatgctac taaatcaaaa
      901 gctcgggaag gaagtgagta acctggagaa aggcatgcct gtacgttttt gatgtgtatg
      961 tctttgactt tcgtcatttg ctttgattca ggtatttctt cagccactgc ttgccaactt
     1021 tctggcattt gagcaaggtc attgctattt tttctattgt attcattatg ccagtccatt
     1081 atccaagaaa aaggaaattg tacatcaatc atattcagat tttctaccct gatattttcg
     1141 atcactcccc ctctttcttt tgaagatttc attcgaatac cacagtctga ctgaaaaaaa
     1201 ttgatatcgt gaatatatac gtcactaact ccagcactaa cttcgctacc aatagttaca
     1261 ccgtatcctg aacggatttt acatctagca acctcgattc ttgatgattt tcgattgact
     1321 cttgcaccat tgccatctct gccagactta atagcaatac aatcatcacc acaggccaaa
     1381 tcacattcat agacacgcac atttgttgaa gaatcaatat caatcccatc tgtacttggt
     1441 ccatcattgt tgcggatgat tactttttct actaatacat cgttagaata agtgatttgc
     1501 aagttccaaa aacctgattt ctgaaaaatc aaatcacgaa caacaacatg ttctgcatga
     1561 tacagtaaac aagcgcgtgg acgtttaata gcataatccg caatccaacg aagtcccttt
     1621 tgatcgtatt cagctcttgt cccgcttttt tgatcttttc cccaatacag ctcccaccaa
     1681 tggtctcctt gaccgtcgat tattcctttt ccttctataa gaatatcttt cgccgagaga
     1741 acattcaaaa tcgctgctgg ccatttcatt tctacgccgg ctactcgtgt atcgatttct
     1801 ggaaatgctt cgatatctgt tctccccttg aggactgcac cttcttcaaa gataagatga
     1861 ctcttatctt ttaaaaaaag ggctccgact aaataaatgc cttttggaat aaatacaggt
     1921 aagtgttctt tcgcaccttg atcgattgct ttttgcagta ttattgtatc atcttgttga
     1981 gtgtcgtttg ttttaaagtt caaagcattg ataatccctt tttttgtccg caatacgttt
     2041 ctccctatct ttcattgatt tttttcaatt cttcaccaat aaatacagtt tttgaacgat
     2101 tcagtgggta acctactagc aaaatgattg cggatacaaa taagcagaat gctggtaaca
     2161 agtttgccat cgtataaatc ctttcttgta ctaaaactga ttggatggct ccacctgttg
     2221 tagacgattg gtaaccaatg agtgccagca taaatccgcc taccgctcct gcacatgctt
     2281 gtcctacttt tctcgcaaaa gaatttactc catatactgt tccatcttct cttaagcctg
     2341 tcttatattg atgataatca atcacgtcag taatgaatgc ccagaccatc aaattgaata
     2401 aaccagatcc taaagtagcg ataaataaaa gaatcagata tacccatgca tttgttatat
     2461 gaaggaaata catcagcaag tacatacatg aagaagcaag taacgcaaat acgcttgctt
     2521 cctttttacc aaataatttt gtcagctttg tagcaaatgg tgctaaaact actactgttc
     2581 cgtaagtaaa taaaagagca acagacattg cttgtttatt atggaagtaa tcattgaata
     2641 aataagtcat attcgtccca gctaaaatct gattgatcac aatgaaaatg tcaacaatga
     2701 ctaaaataat cagtgctcga ttagctaaca aaccttttac caactgagaa gcagatactt
     2761 tttcgttttt gacaactctt actctttcag tagtcaattt acaagttaac atatatgcga
     2821 caaatgctat tgcagcacat ccgattgaaa tccaaaagaa atgattccct gaaacgactt
     2881 gttgtccact cttgttatgg gtatacatta aaatcggaat gaagaatcct gtagttgatc
     2941 cgccaatcgc agaaccaatg cttcggaacg tggacaacga ggtgcggtca ttaggatttg
     3001 agctaattgc tgctgccatt gctccatacg ggatattgat tgttgagaga aaaatcccat
     3061 aaacaatgta tgaaacaaaa atataagcaa tcttcaccgt cattggaaga tgttggacga
     3121 atggcaaaaa caaaatgaca gtcactaagc aaaatggata cttcattttt ctgatccaag
     3181 gaataaatcg gccagtttct gttaactctg agacatcaca taatcttcca accatcacat
     3241 cagcaaacgc atccaaaatt ctcgaaatca agaataacgt acctacgatt gcccctgaaa
     3301 cgccaagaac attcgtataa taaatcatca agaaactatt gactaaacct aaaataaaac
     3361 agttacctag atcaccgaac atgtagccta tcttatcgga aatcccaaat tccttttttt
     3421 ctttttttac tgtatttgct gtaacagcat tttgtgaaag catttgaatt cctcctacct
     3481 cttctagtaa acgttttcaa aattataact ctttttttct tgtttttata ttcacaatgt
     3541 tggcttttta ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat
     3601 gcataagatt gataactttt tttacataac aatatataca aacaaaaaaa ttcactaact
     3661 agtgaaaata agttagtgaa cctcatttat taaagaatta aattacagat gatcttttcc
     3721 atcatacttg aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac
     3781 tgctatcccg ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg
     3841 acctactttc ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt
     3901 ctttacagtg actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat
     3961 ttgtgctact ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca
     4021 agtaacataa caataaatat aattggattc atttaagtac agtaccagtc cagccatctg
     4081 attaaaatta ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg
     4141 ccgaatagct aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg
     4201 taaaaaacct ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc
     4261 attccagttc ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg
     4321 ttgtacctct tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt
     4381 aagtcttaac caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa
     4441 aatagccgca ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg
     4501 ggtttgtact aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc
     4561 tgtcagcata ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca
     4621 aactgtaact gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc
     4681 gttacgaaaa tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt
     4741 atagatggga ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct
     4801 ggatttattc ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc
     4861 cgtttcaaga tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc
     4921 ggaccaagga cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg
     4981 acggcctgca cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat
     5041 ctgtggcgcc catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa
     5101 aatatccgtt gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg
     5161 aagccattca aaagaggaga caataatgta ataccgctct ttcactctta atatattagg
     5221 atctggattg aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc
     5281 tccttttctc attcttactc gatataaact gaatgcccca gctgctaaaa aagcaagtga
     5341 aataaacaac acaacggcaa acagaccagt catgtcctgt aataatccaa atgtattttc
     5401 taaagacaaa gttgaatgat ctacagcaag cgactgataa ctttttttaa actctgacgc
     5461 agacatatta cttaaagtaa ctgctaaccc gccaaagaaa atcaaagata gactgccacc
     5521 gaccattcct agcaatagtt cccattttct tttcatttct gaaccccctg cttttctaaa
     5581 tgtatccatt caggtctttt gatcgatgtt tcataattgt tattacgttc agagaatttg
     5641 acagtctgtt catttttctt gtcatcccaa ttatcccaac cagttgggtg aacatgccca
     5701 cctatcttac acgcttcaaa taagacattt gcatacggac gccacggtct tcctaaatag
     5761 acgtttctag tatcaggttc tgcagtcaag gtacagcttt caaaacgata ccctgttttt
     5821 tgatttttcg gcgtagatgc tgctgtgatg tatccaggat ttttactggc taagctatgg
     5881 atatgacact gatgaaaaac ggcatctgcc ccaccaaata tgaaatccac cgttccttca
     5941 atccagcaat attcataggt ctgacgacaa tactcaggat tggcagaagc cggtgtacaa
     6001 aaaggagtac catctttttg caaatcaggc aacggacccg tgcataaggt atcctgataa
     6061 ccagatagtc gacagttttt taaagatacg cgatctcctg aattgaataa agcaacagcc
     6121 tgtcctacct tttctcctgg acctgctgta ttttcgatgt gaagattttc taatacgaca
     6181 ttcgatcctt ctaagaaaaa agtagcactt cggaaagtcc ctaactggct accgtcaaga
     6241 tactcctgtt ttgcaaaacg attaccggag ataactactg ttcctgctcc cacaagttta
     6301 aaattggata atctgctttc tatatattca tcatatcttc cttcttttac tatcaagacc
     6361 tttggatggc tatccggcag gcctgccaaa taatcgattc cttcttggat agtttcaaaa
     6421 tccgtcgggg tatctatagc attctttcca attatcagtt tgtctttcat cctcataaat
     6481 gctcccttat gaattgtttc caaatatttc tcatttcttg tgtttccata tgaccgcctg
     6541 ttaaagaaaa agaagaaaag ttttctggac tacccattga ttgatacttt ttgtccagcc
     6601 cttttctcag cttcagtacg ccttcgtcca agcacatcgt atcgtttttt ccaactaagc
     6661 taagacgagg gcgcggagcg attttttctt ggattgctaa cgttgtataa gctgtcaata
     6721 gatttggcac atagtaataa aacccatgat gatctaggcg gcgatgtagc aaaagtgttt
     6781 cgatgtccac ctgtgccgct agatcaatac aaactttgac acgagaatcc aatgctgtca
     6841 cccaccagct taataagcct cccatagaaa gaccaatcgt tgcgagccgt tgagtatctg
     6901 tgtcttccct tgtctccaaa taatcgatca agctgatcac atcataaata cgcattcccc
     6961 ataacgtttt tcctgttaat aaaaattgtt tgaacaactc gctttcagaa atacctccgc
     7021 gttcttcaaa tccccaagca tcaatcgacc aaacagcata tcccattcct gtcagtactt
     7081 ctgcaaaaga tggagaagct aagtaaggag caccatggat caattcactt ttcccgactg
     7141 aaaaatctcc gccgtgagaa tgcaggtaaa ggaccgtcgg aaacggtcct tctttttctg
     7201 gataagcata gagggctggt actgtttcca attcatttaa gtacaattga tagtattcaa
     7261 gcataaaacc gttttgtttt tctcggtata atcgttttcc actaggggta tgtctgctag
     7321 atggagcacc taaaaaatca ttcaactgca taagcaaagt ttttacgaac aaccagttca
     7381 tccatatttt ctgattgatt tgattggaat ttctcaatac tgatttcttt agtattttct
     7441 aatagaaatg ctggtccctc gtgattttca atcgacatct ggttaaagga aaccttaccc
     7501 gcaaagccaa tataaaatcc ttgtctgctc atttcctcta ttcctgtcat catagcagga
     7561 acaccgggaa ctgcatcttg ggccatcgag acatcgatat tgtcaaatgt gatttcttgt
     7621 atgaattgtt ccgctaatcc ataaataaaa cctgcagaag catggacatt ccttgccgtg
     7681 atattcgaaa aatggatcct tctgaatgct gatgtccgtt catctatcgg ataagccttt
     7741 ttttcccata catacggttc tttccctcgc ggaccacaga aataataaag attcagtata
     7801 aatggacaca tcacattatc catgactata ttggatactc gaatatcttc aacgatcccg
     7861 cctcgacctc gtctagattt caatcgtatt cctcgatcag tttcttggaa gatacaattt
     7921 gaaatggtaa tattacgaat actaccactc atttcacttc ctaatacgac accgccatgt
     7981 ccatggacca tcgtgcaatt tgtgatcgtg atattttcgc aagcgatcct ttcgtacgta
     8041 tcttcagtcc ctgccttgat tgcaatacag tcatctccca cgtcaatatg gcaattacta
     8101 atacgcacat ttttgcatga ttctggatca attccgtctg tatttggtga atccgctggg
     8161 ttcaagatcg tcaagttatc aaacgttgca ttactgcaaa gaatcgggtt gatcgtccaa
     8221 cttggtgact ggatcaattt aatatctttc acagtaattc gatgacagtt atggaaactc
     8281 atcaatttag gacgaggata agcgagatta tctggttcat ttctaaaagt atgccaccat
     8341 ttttttccat ttccatctaa tgtaccgaaa ccagtgacag agatgttctc tacattttga
     8401 gcatagatac aggaagcata aacttttcga tggactcctt cccaacgtga atgaacaaca
     8461 ggataatctt ttggatcgtc agagaatttc agaaccgctc ctgcgcttaa atgaagttcg
     8521 acattgcttt ttaaaaataa agctcctgtt agaaattcac ctgctggaac aactacggtc
     8581 ccgcctccgt cacttgctgc agcatcaatg gcttgttgta ttgcttcagt attcaattca
     8641 tcaattgatg cgccaaatgt caaaatatca tacatattta taatgcctcc acttcaatca
     8701 atgcttgcaa gaatgcacca acgccttttt gatcattaca aataattggt tcactgatgt
     8761 agtaagcata tgaaccatct cgctgatctg ctccgcctaa ccctgctact tgacagtttt
     8821 tattcaagtt cagccagcct tcttttgttt ccagaacaaa ttcatcaagt aatccttgat
     8881 gtgatttctt cacttgtgga agccattctt ctttgttcag cagtcccaaa cgtattcctt
     8941 tagccattgc gtaaacaaac atactactgc cggaagcttc cagataattt cctttccgtt
     9001 ttgtttctgt tgttacttga taccatgtcc cgctgttttc ttcttgatat ggaagtaatg
     9061 cagccagtgt cttttctaga atttctttca gcaaagaaca atctgtttct tcatgaagta
     9121 attcataggt atctgctgca gccatcaaat accagccaat cgatcggctc caaaaatgat
     9181 gggatagacc tgtttttgga tcagcccatg gttggacctt cttttcatcc catgcatgaa
     9241 tcagcaaatt tgtttttgga tcgaatagat gtttataact gatttcaaac tgtctcaaaa
     9301 catcttggta atctttttct tcactaaaat tctggataaa ttcagcataa aaaggagaac
     9361 ccatatataa gccatccaac caaatttgat aaggataaat ttctttatgc caaaaagctc
     9421 cttcggatgt gcgtggatgg tttgccaact gctgggaaag gagttctgct gcttttttat
     9481 atttttcttc gcctgtttct ttgtacaata aaaagaaaag ctttcctgta tttacatggt
     9541 cgatattgta ttcttcaacg ctgtagccac ggatcgttcc atcttcttgc acgaaatagt
     9601 cgatattgtc ttttatgaaa tcaaaataaa ttttttcttg ggttcgttcc caaagtaatt
     9661 ggaacccttt aagtacgacg ccatagtcat aagaccattt tcctttatac cacttttctt
     9721 catataagcg aggtgttctt tccatgatac tttttgctaa tttctttgac cagttttcca
     9781 ttatttgctc tcctttaaaa attaaaatag gatttgggac aaataccaag aagtatttgt
     9841 cccaatcgtc tatattactg tgccgctgct ttgtatgctt cctcgtactc ttgtgtgatt
     9901 cgatcgcctc cttgtgcttt ccagtcttct acagcttttt tgaatccatc ttcgtcaagc
     9961 tgtcccatga tgtattgcac cgtagcatca atgacgattt tttcaatttc tgatcctact
    10021 tcattgttcg tcggtgaatc caatggcaca gaaggatcca ttacagcaaa ttcttcattt
    10081 tctttgatca attgattaga gaattctttt tcagggtctg tatctttgaa agagaacgtc
    10141 acttcactag gacgagagct tgataacggt tgcacctctt gttgccaaag atccatatcg
    10201 ataaattcga tagaaccatc atcattcaat tcataatgcg ttccttcgat accgccagtc
    10261 atcaatttat acacatcttc ttctaaaaga tcatcaacaa attgaagtaa tttcttcaat
    10321 tgtgcttcgt cttttacttg agatttggga aatgctagca agccgccaat accatttcct
    10381 tctgaccaaa catgataacc gccgccttca ccatctgaga tcttgtttac aggcactaat
    10441 tccatatcat cttgaatacc ttgacccatc gtcttcagat tcttgatatc caccattcca
    10501 gtgtagattc cagttcttcc ttgtgcaaat tgttcttgct gatcagtctt ttgggtaact
    10561 gcaaagtctt gtgccaaata gccattctca aatagatctc ttgaaaaatt caaagctttc
    10621 atatactctt ctgtatcaaa ttctggagtg aatttgccat tatcgtctac agcccagcca
    10681 tttggtgctc caaaataaga agtgaaaagt ttaaagctgg tataacgaat atcactaggc
    10741 gcacgatcgc caaaaccaac tgtgtcgtcc acaccatttc catcaggatc gttttctgta
    10801 aattgtttag ctacttcata aagctcatcc atggtagttg gaacatctag tcctagatta
    10861 tccaaccagt cttttcgaat gactaagcca gcacgagcgt agtctttttg tatcggtacg
    10921 ccgtacaagg caccgtcgat actcgcagct tcgattctat cttcagaaat ttgacttaaa
    10981 tttttatagt cttttaaata ttctgataca tcccagaata cgcctgattt taacgcattg
    11041 cggacagaag agtttttcaa catcgcaatt tgaagtgtaa cgatatctcc tagttcatca
    11101 gaagccaatg cagttgttaa tctttcttct tttgaagcat caggaatcca gttgaatgtc
    11161 agttccgtat cggttttttc ttcgatcaag ttcacaatcg tgtctgtagg cggtgaagca
    11221 tgatgcaaaa tatttaacca agtgatttta gcttttccat cttctgtctc tgaagaactg
    11281 ttacttgaac tgctgccgca tgcggcaagt gctagaattc ctgcaaaact aagacctaat
    11341 tttacgagcg ttgctttttt cataaatttt ctccttcact gttttaataa tatttttgtt
    11401 ttcttctgtt ttttctgtat atgagttctt ccattccaaa tggcaaaata tagaatttgc
    11461 tgtccacgtg actgcggaaa agaagagact gccaccgata aaggttaaaa cacctggcat
    11521 tgtttggatg atcatcagat aaatccctac catacttaat atcaacacta atgagtaatg
    11581 aagatgtgag aatccgaata aaaatgccat tttcattttg aaaaaatatc ctttccaatc
    11641 ataatgagcc atgattggga aaatatagag tgctgagagc acatatatga acgcagcaat
    11701 atacaaagcg atcctaagat accaattgtg tatgaaagaa agatccacaa gtaaaacaat
    11761 cccgatggcg ccatatatcc agctcatcag caaggtttca cgatagtttt ctttaaaata
    11821 tgtgaagtac gttttcgtca aaggaaacgt ttcttttgag cgcagccatt ttctaatcac
    11881 tgcaaccatc gcatacgatg caggaccgaa agtaaaaact cccaaacctg cgatgactaa
    11941 accgccccac agaaaattca aataaaccaa ctgtaaaata cgatttagcc ttccgtttat
    12001 tttatctaat gactgcagca tatctttccc tctctttcta tcttagtaaa cgccgtcttc
    12061 tcctagcttt ttggcaatct tattagctaa aattaccata aacaatccta cgaccccttt
    12121 aaacaatccc attgctgtac tgaaacttaa ctgtccattc ttcaatccag ctgtataaat
    12181 ataggtgtcg aaaatttctg caacgcttcg attcaatgaa ttcaataaca agtacatatg
    12241 ctcgaaccct aagtctaatg tctgaccgat ttttagaatc agcaaggtaa tgattactgg
    12301 acgaattgcc ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc catccatttc
    12361 tgcagcttca tacaactgtg tatcgactgc agttatcgct gaaaggtaga tgatcgtaga
    12421 ccagcccaac tctttccaga tcacttgtcc gatatatact gtgcgtgtcc attcagggga
    12481 tgttagaaaa ctgatttttt caaatcccat tgttgcaagg aattcattca atacccctcc
    12541 gtcaacattc aaaaatacat aagtaatcga aacaatgatt acccaagaca tgaagtgagg
    12601 gatgtaaatg atcgtttgaa tcccgttttt caatcgtttg ttcgttactt catttaatag
    12661 taaagacaaa atgattggca tcggaaaaaa cacgaagata tttaatccga acaagatcaa
    12721 cgtatttctt aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga aatgtttgaa
    12781 accgacccaa ggacttccca ttattcctaa aaatggttga taatcttgaa aagcaataac
    12841 gagtcccccc ataggaagat acctaaaaat caagaaataa aggactcccg gcagaatcat
    12901 cacatataaa aatttattcg ttttgattcc tgcaattatt ttttttcgtc ttctgagctt
    12961 ttcttctttt ttcgatagcg tcagttgaaa tggttcttgt ttctttacac tttccataag
    13021 cccctcctta gttttgtgaa tgtttgctgc ttatgacttc atgatagata actatctgaa
    13081 ttacgtatat aatcattttt atcgtaagta aaaacgctta catagcaatg atttttgtgc
    13141 tatacaaaac gataaagtgt attttttcac aaataaacta cgcatagcaa accttctatt
    13201 tttttctatc tgacacttag cataggaaat gattatatac gtaatctttc accgatgata
    13261 aattgaacat atagaaacag aaacatttaa tttattttga ggaggagcct atctatatga
    13321 aaaagaaagt ctctatagga gaaagaagtt ttacactttt caacaatacg tttctaattt
    13381 tgctagcttt gatctgtatc gttccttttt taaatatcat cgctacatct tttgcttcta
    13441 cacaagaagt cgtcgcgaaa aagtttatcc tttttcctac taccttttct ttagacgcgt
    13501 atcgctacat tctttctacg ccgaccattt ttagagcact tgctgtttcg attggtgtta
    13561 caggtgtcgg aacgatcgtc agtatgtgtg caacttcact gatggcctac ggtctttcca
    13621 gaaagtattt gtttggtcga ggtttcgtca actttcttgt tgtattttct atgttgttca
    13681 gcggggggat gattccaacg ttcttagttg ttcgttctct agggctggtc aattcttact
    13741 gggccatgat cctgcctgtg gctgttaatg caatgaatat gatcattatg agaaactttt
    13801 tccaagcctt gccggatagt ttggaagaat cagcaaaaat ggatggttgc acagattttg
    13861 gtgtattttt caaaattatg ctgccattag ctttaccgtc tattgctact atctcgcttt
    13921 tttatgctgt tacttactgg aacacttata tgactgctat tttgtatatc aacgattcat
    13981 ctaaatggcc gatacagatt cttttacgcc aaatcgtcat tgtatcaagt gggatgcaag
    14041 cagaaagtag tgccgttgat gtgattccac cagcacaaac agttaaaatg gctgttatcg
    14101 ttattgcaac agtgcctatg ctgattgttt atccttttgt tcagaaatat tttgtcaaag
    14161 gagcactcgt aggttctgtc aaagggtgat tatgtactga acgcaaaatg gtagttgtgt
    14221 cagaagtggt cagcttcaag aaataagaag gcatttacga aaattgcttc ttaaattttt
    14281 gtgaaatttc ggcttatttt tcgaagaatc tactcctgga acaccgtgta tcagaggttg
    14341 tgaaagagac gttcagcctt gagcgatgat aaaaactctc acaaacattg cgacgagtaa
    14401 cccacaggag caataagaag cgattttgaa aaatagcttc ccatatttca caaaattatg
    14461 ccttattgtc gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa
    14521 gacttttgtc acagttcttt ttgcgtgctt agccttgaaa tttaactatc cataaataca
    14581 aatactaggg ttattctaac ttcttttatt gcttgaatca gtccacaaga aaaacagcct
    14641 cttgattaaa cggcgtttat cttgttgatt ctgcggcaat aaaatcagcc agcagcaaaa
    14701 catgaagtgc tgttttttgc tgtcctcttt tattgcttga atcagatcac aagaaaaaca
    14761 gcctcttgat tcacaaatat ttttttcgat attctcccgg cgtaatcgat tccttctttt
    14821 taaagaaacg aataaaattt tgcggatttc gatattgcaa tctttctgca atctctttta
    14881 cggagaggtt agtctctttc aaccattttt ttgctacctc taaacgatga ttttgaacaa
    14941 aatcaccaaa tttttctcct gtttcctttt taaatacatt acttaaatag ttagggttat
    15001 aatgcaacct atcagcgatc acatctaaag acagatcacg atcatattct gtgtagacga
    15061 tatgcataat attctccgac aaatttttaa attcatgatc agtgattgct tgagagcatt
    15121 ctgtgattgg cagaactaat tgttctaaaa caagtttttc tagttctttg ggatgataat
    15181 tgttcagtgc ttttacataa atttgtttca tgttatcaaa gttttttgaa tctactccca
    15241 ataactgtcc taattgaacg agttcattga tcaagcggac cgaaactact tcccgactca
    15301 aaggattttt attttgtgca aacaatgcat caatcaattc atgtactaaa ttcttcactt
    15361 cctgatttcc agaacgaatg gcttcaaaca atcgattttg ttcttctatc ggatatctaa
    15421 taagtgtttt ttcatgagca gcaggaacga tttcctgata aaaaataatt gaacttggac
    15481 cagtatttac acgataatac agggcttctt ttgcccgatc aaccgattgc ttgctttcat
    15541 tcagcgtttc gaaccggtta ctgatgccta tactgatagt taaattcaag tattctttta
    15601 cacgtttttg aatgagtcgg caatactcca tgactttttt gcttgcttct gcatcatctt
    15661 tatccattcg ataaatagtg gcttgcattt cttcattcaa aacgatcggc agcattcgat
    15721 tttcctttgg cacaatttcc gtgatcatgt tgttgatcgc taaaagtagc aaatcacgct
    15781 ccccaccgtg actatcttct aacagatcga tttgaaccaa agctgtataa tagcagtcat
    15841 tttgagaact ataaccaaat tgctgcaacc gctgatctag ttctcgcctt tctactcgat
    15901 ttctaaataa actaagtaca aatagcgttt ccagctgtgg cttttgggta actaagtgtg
    15961 cacttaacgc ttctttttct cctatgatcc gactgactga ttgagcgacc aaatctagtt
    16021 ctttcccact gaatcctttt gatttaagat tcagacgttc ctgtatttga gaaatcggac
    16081 gggcaaatct gtccgaaaat aagtaggaaa aaagaccaat gagtcctacc agtgttaaag
    16141 acactgcaac tagcccgatt ctcagattgc ggatcattgc gccaattgca ctagagtcaa
    16201 cttctaatct gtaaatccaa ttattataat ctgatttcag tactatatac gttttatctt
    16261 tttctcttat cactttcagc tgattgtaat ctagatccac gtccgttaat acagttggtt
    16321 gctctttatt ttttactgtc tgagtagtaa aaaggttgcc aacttcgttt tcaatagaaa
    16381 gtatgtcttc tccactttca ccaacaactt gttctattgt atgtttctct atcgtagcaa
    16441 ttcccaatgc atatttttct ttttcaaaca ttggcaaaga aatcaccatt tcgattcctt
    16501 tgtttttctt ttgccaaaat aaattatttt tgttatttat gtacttctgt tgatactttt
    16561 caacttcctc ttcggtcagt tgactcaacg atccattgat tactccccat ttaccagcca
    16621 aactgactaa atcataatta ttatcatcca taacaatcgt ttctataaaa tttagttctt
    16681 tcctgatttc agaataagtg gagtaatctg tatatgtcag cggatgcttg aatatgtccg
    16741 agaaattttt ggtcgaacca tacgtagaaa acgaatattc aatcgttcta attttccatt
    16801 caacgttagt tttaacttgc ctgagcaaat tctttttgtc tgttaaataa ctcgattcga
    16861 tcgattcata ggtattctgg taaatgaata aactaaatcc tatgacaagt acggctccta
    16921 ataaaaacat cggtaaaaat attttataaa atactgtact tttcttcatt ttcttcctct
    16981 cctcctttta aaataaaagg ctttcatttt tctgtaaaat tgatactcat ctaattaaat
    17041 gagcattccc cgctttcaag aatagcataa ttaaaaataa aaatccaagt aatttttaac
    17101 gtgtgcaatc ttttcatatc aaagatctat tgacttatcg tagttatcgt aatatgatga
    17161 atgcgttaac aaaaagtgaa aaagtaagga aaatgatgga atggtaacga tcaaagatat
    17221 cgctaaagca gctggcgttt ctcatacgac agtttctcgt gctttgaata acagcagttt
    17281 aataaaaaat gaaacaaaac aaaaaatacg acaactagct aaggaaatga attattcgcc
    17341 aaactatagt gcaaaaggtt tagtgaatca aaaaaaatat ctaatcggac tatttttctc
    17401 aagtttgcat aaaggaactt catcaaattt cctagcagat tccattgcag gcatccattc
    17461 agtattagac acaaattaca gtttatctgt tgaaagtatc gatgttgttg atttaacaga
    17521 aatcaacctg caacgctacg atggactgat cgttatgagt caaagtgatg atgatcagcc
    17581 tttcatcgac tatctcaaag aaaaagaatt tcctttcgtt gttgtcaatc gtcacatcaa
    17641 tgatccagcg gttatcaacg tcactgccga tgatgcggcc ggtgttatgc aggcagtaga
    17701 ttacggtatt ggatgcggac atacgaagat cgcttatatt ggcggaaaga aaaattttcg
    17761 ttcgacaaaa gaacgggaaa aaggggtatt gaaaagtcta aaaaaagcga gcatccctgt
    17821 caatctagcc tattttttaa caggagatta cagcttagaa agcggcctcc aaaacatgga
    17881 atacctatta tcactttctg acctgcctac tctggttttt tgcggcaacg acgacatagc
    17941 gattggtgca ttgagagctg tacatttagc tggcctccgt gttccccaga acatttctct
    18001 gatcgggttc gatgattctc cagtcgtctc ctatctcaat ccgccgctta ccaccgtacg
    18061 taagcctttg aaagaaatgt gtcaagaagg aacaaagctt ttactagatt tgatcgaagg
    18121 caggacgatc gaacaatcta aaattgagct ctccaccttg ttgatccttc gtgagtcgat
    18181 caattacttg taaactacaa gtaattttat ataaaaatgt taacgtgtgc ataacacaga
    18241 aaggaagttt atatgaaaaa actgacaaaa gaattagtga aaaaaaacga agcacctgta
    18301 aaagttcttc agtttggtga aggcaatttt atgcgtggat ttattgattg gcagattcag
    18361 cagctgaata accaacaatt gtttaaaggt aatgttgcaa tcattcaacc tttagaacaa
    18421 ggtcttggaa atatgatgaa ccagcaagat aatttatata cggtcatttt acaaggactc
    18481 agcaatggcg aaatcgttga tacttccgag attatcaccg tagtagaaca aatgatcaat
    18541 ccttatgatg aatgggatgc ttatctatct ctagcagaaa atgacgactt ggcctatatc
    18601 gtctccaata caacagaggc aggaatccaa ttcgtggaga ctgataattt aacagatact
    18661 cccccacaga gcttcccggg caaactgaca gcctttttat accgacgttt caaattaaac
    18721 aaagctggct ttaccatcat tccttgcgaa ctgattgaca gaaacggcga acgtctaaaa
    18781 gaaatcattt tacgctacgc agaaaaatgg gatttagaac ctgcttttgt tgagtggtta
    18841 gaaaaagaaa atgttttctg ttgtagttta gttgatcgca tcgtcccagg ttatcctcgc
    18901 gaagatgcag gtgttttcaa tcaacggcat aactatgaag atcaattgat ggtcaaagca
    18961 gaaccattca tgctttgggt gatcgaaggg cctaaagaat tggagaatca actaccattc
    19021 aaacaggctg gcttgaatgt catcatcaca gatgatatga ctccttatcg tgaacgtaag
    19081 gtgcatttgc ttaatggacc gcatacagcg atggtccctc ttgctttgtt ggcggaattg
    19141 gaaactgtag aagaagtaat gaaagatcct ttattctccg catatgttga tcagttattc
    19201 aatctagaat tgattcctat gctttcttta cctaaagatg aattagccat ttatgctgac
    19261 caaatcaaag aacgtttctt gaatcctttt gcccatcaca aattagaagc gatttcatta
    19321 aatagtgttt caaagttcag cacccgtctg cttccagtgt tcaagaagta tatcgaagaa
    19381 caaaatcagg taccaccgtt aatcactgtt tcactagctg cgctacttct tatgtatcga
    19441 ggcgatcaag taaagccaca tgatgatgaa aagacgataa gcgaatttac tgatgcttgg
    19501 tctgataatg gaacagctat tcctcgatta ttgcaaaacg ctgcgctttg gggagaagat
    19561 ttgtctcaaa taccgaatgt gacagataca gttcaagaaa tagctgcaaa gatcgatcaa
    19621 aaaggtgtcc ggtcagtatt acaagaaatg aaaggaacga tggtccatgc ttgaaaccct
    19681 gcaaccaaca atgaaattaa atcgacgaga cagtgtggtc gtcgcattaa cccctattcc
    19741 aaaacaaaca caactgacga ttgatcagca aacgatcacc actcttgaag atatcccgca
    19801 aggtcataag attgcattgg gtgatttgaa agctggagac aacgtgatca aatatggcta
    19861 tccaatcggt catgtgacga cagacgtaac tgccggtcaa tggctgcaca ctcataatgt
    19921 aaaaaccaat ctttctggtg aattggatta tcgatatgaa aaagatgtcc atccaagtca
    19981 ttaccttttt gagaaccgaa catttcaagg ttacttgcga aaaaatggaa aagtagggat
    20041 tcgtaatgat ttgtttttag tacctaccgt tgggtgtgtg aatggcattg ctgaactgat
    20101 cgtcaaacag ttcaaggaaa aacatccaga tctgggacag tttgaccata ttacgatttt
    20161 aaagcatcct tatggctgtt cgcagcttgg aaaagatcat caaaatactc gagaaatttt
    20221 agcggacgca gtaaatcatc ccaatgccgg aggagtttta gtatttggat taggttgtga
    20281 gaacaataca gttcctgaat tcaaaaaaat tttaggtgaa tatgatgaag aacgagtgaa
    20341 atttctagtg gctcaagatg tttacgatga gatcgaacaa ggtgtggctc tattagaaga
    20401 gcttttaaca gcggcggaaa atgaccagcg catatcagtc ccgctctcta agttgaatgt
    20461 cggtttgaaa tgcggcggtt ctgatggtct atcgggaata actgctaatc ctttacttgg
    20521 tgcattttct gactacttga ttgctcaagg aggcagcacg atcttaacag aagtgccaga
    20581 aatgtttggc gcagaacaag tattgatggc acgagcggaa aatgaagaag tttttgactc
    20641 aatcgttgac ttgatcaatg acttcaaaca gtatttcttg tcttacgggg aaccagttta
    20701 cgaaaaccca tctcctggta ataaagcagg tgggatcact acattagaag ataagtcttt
    20761 aggctgcacg caaaaatcag gaagctctcc tgtcgttgat gtgctacaat atggcgaaaa
    20821 aatcagaaaa ccagggctta gtttattgca agctcccggg aatgatcttg tagctgcctc
    20881 tgctcttgct tcatccgatt gccagttggt cttgtttacc acaggaagag ggactccgtt
    20941 tggctcatat gtcccaacgg tcaaggtatc tacgaataca actctttttg aacgcaaagg
    21001 tcattggatg gattttaatg caggcgtctt attagaacaa ccaatggaag taatcttaga
    21061 atcgtttgtt caaaaaatca tcgctgtagc tagtggagaa gaaacgaaaa atgaacaaaa
    21121 tgacgttcga gaaattgcca tttttaaaaa cggagtaaca ctttgatttt cataaaacag
    21181 tataaaaaga aagaaggaag atttatgttt ttatctgatg attttttatt aaaagacgat
    21241 tgggcgaaaa agctttacca ttcctacgca aaaaacatgc ccattattga ttatcactgt
    21301 cacttagatc caaaagaaat ttacgaaaac aacaatttca gcaatctgac agaagcttgg
    21361 ttaggaggcg atcattacaa atggcgcttg atgcgagctt gcggcgtgcc tgaagaaaaa
    21421 atcacaggaa atgcttctga ttttgaaaaa tttttagctt ggtgtcaaac cgtaccaaaa
    21481 attatcggaa atccgcttta cagttggtca catcttgaat tgaagcgttt cttcggtatt
    21541 gacttgttga taaatgaaga aaatgcagag aaaatttgga cacaagcaaa tgaaattttg
    21601 gcagctcctg attttcgccg tcgggaaatc gcaaaaaaat cgaatgtaga agtgatttgt
    21661 acaacagatg atcccgcaga cgacttgcac tatcatcagt tgcttgctca tgaagaacca
    21721 gatttgaagg ttctaccttc attccgtcca gataaagcac tcaatattga aaaagaagga
    21781 tttaatgcat gggtcacact actggaagaa gcagctgacc aaacgatcac cacctatcaa
    21841 gaactgatag aagtcttagg gcagcggata gaatacttcc atcaacatgg atgtcgtgtt
    21901 tccgaccacg ctttagacat cttacgctat aaagaagcgg atgaaacagt attagaagag
    21961 atttttcaaa aagcacgagc taacaaacta ttatcttcag atgaaatcga tgcttatctt
    22021 acagaaacac tgatacattt gacccacttc tatcattctc ataattggac gatgcagctg
    22081 cacatccatg cttatcgtaa ctgtaataca caaatgttgg aaaaattagg gcccgatacc
    22141 ggctatgatg gaatgaatga tctttctttg actattcctc tgcaaaaatt attgaaccgt
    22201 gcagaagaaa cggatcagtt gccaaagacg atcctttatt cattgaatcc gaatgactat
    22261 cctgcgattt tagctttgat gggctgtttc caaaaagaac agaatggaaa actgcagtta
    22321 ggttctggat ggtggtacaa cgatactcga gctggtatgc gcgatcaatt gacacaattt
    22381 gctgatggta gtgctttggg gaattttgtc ggcatgctga cagattcaag aagtttcctg
    22441 tcctacacta gacatgaata tttccgtcgt gttctttgtg aatttttagg cgaaatggtc
    22501 atgcgaggag aagcacctga agatgaacaa cttttaggaa gtctagttca agcgattagt
    22561 tatacaaatg ctaaaaatta ctttggcttt tttgcctaat tgattaatag taacagtaac
    22621 gaatcaaaaa caggatagtg aaataacgga atccgtttat ccacgtttta gagaagaaga
    22681 cggaccaa
//
LOCUS       DACDTH010000043        22077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDTH010000043 DACDTH010000000
VERSION     DACDTH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.043"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(105..1568)
                     /locus_tag="IWK54_001773"
     CDS             complement(105..1568)
                     /locus_tag="IWK54_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9593894.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1632..4649)
                     /locus_tag="IWK54_001774"
     CDS             complement(1632..4649)
                     /locus_tag="IWK54_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9593895.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4800..5639)
                     /locus_tag="IWK54_001775"
     CDS             complement(4800..5639)
                     /locus_tag="IWK54_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9593896.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5784..6029
                     /locus_tag="IWK54_001776"
     CDS             5784..6029
                     /locus_tag="IWK54_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593897.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6092..6514
                     /locus_tag="IWK54_001777"
     CDS             6092..6514
                     /locus_tag="IWK54_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593898.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6608..6925)
                     /locus_tag="IWK54_001778"
     CDS             complement(6608..6925)
                     /locus_tag="IWK54_001778"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593899.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(6952..7158)
                     /locus_tag="IWK54_001779"
     CDS             complement(6952..7158)
                     /locus_tag="IWK54_001779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593900.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7139..7321)
                     /locus_tag="IWK54_001780"
     CDS             complement(7139..7321)
                     /locus_tag="IWK54_001780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593901.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7335..7685)
                     /locus_tag="IWK54_001781"
     CDS             complement(7335..7685)
                     /locus_tag="IWK54_001781"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593902.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7711..8013)
                     /locus_tag="IWK54_001782"
     CDS             complement(7711..8013)
                     /locus_tag="IWK54_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593903.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8016..8249)
                     /locus_tag="IWK54_001783"
     CDS             complement(8016..8249)
                     /locus_tag="IWK54_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593904.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9235..9557)
                     /locus_tag="IWK54_001784"
                     /pseudo
     CDS             complement(9235..9557)
                     /locus_tag="IWK54_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9680..10795)
                     /locus_tag="IWK54_001785"
     CDS             complement(9680..10795)
                     /locus_tag="IWK54_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593905.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10801..11199)
                     /locus_tag="IWK54_001786"
     CDS             complement(10801..11199)
                     /locus_tag="IWK54_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593906.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(12228..12614)
                     /locus_tag="IWK54_001787"
     CDS             complement(12228..12614)
                     /locus_tag="IWK54_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9593907.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(12611..13132)
                     /locus_tag="IWK54_001788"
     CDS             complement(12611..13132)
                     /locus_tag="IWK54_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9593908.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(13134..14168)
                     /gene="sppA"
                     /locus_tag="IWK54_001789"
     CDS             complement(13134..14168)
                     /gene="sppA"
                     /locus_tag="IWK54_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9593909.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(14201..15616)
                     /locus_tag="IWK54_001790"
     CDS             complement(14201..15616)
                     /locus_tag="IWK54_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP9593910.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(15585..17738)
                     /locus_tag="IWK54_001791"
     CDS             complement(15585..17738)
                     /locus_tag="IWK54_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP9593911.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            17996..18232
                     /locus_tag="IWK54_001792"
     CDS             17996..18232
                     /locus_tag="IWK54_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9593912.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            18251..18466
                     /locus_tag="IWK54_001793"
     CDS             18251..18466
                     /locus_tag="IWK54_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9593913.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(19169..19921)
                     /locus_tag="IWK54_001794"
     CDS             complement(19169..19921)
                     /locus_tag="IWK54_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9593914.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(19936..21183)
                     /locus_tag="IWK54_001795"
     CDS             complement(19936..21183)
                     /locus_tag="IWK54_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9593915.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(21259..21405)
                     /locus_tag="IWK54_001796"
     CDS             complement(21259..21405)
                     /locus_tag="IWK54_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP9593916.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(21509..21820)
                     /locus_tag="IWK54_001797"
     CDS             complement(21509..21820)
                     /locus_tag="IWK54_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9593917.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(21822..22019)
                     /locus_tag="IWK54_001798"
     CDS             complement(21822..22019)
                     /locus_tag="IWK54_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9593918.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT         6511 a         4221 c         3217 g         8128 t
ORIGIN      
        1 gagatttcaa attaaaaaac gaaaatgaga gatacaaata tatcaaatat ttaaaaagta
       61 accacatttt ctataccact ccttatcaga aacagcctta ttctttagaa taagactgtt
      121 tctgggttga ttaatttgtc ttttgttaaa ctaccatcta aggcaagttc taaatgtaaa
      181 tgtggtcctg tactatttcc tgttgaaccc acataaccaa taatttgtcc ttgttcaact
      241 gtttgtccta cgttcacgca atattcctgt tggtgcgcat aaagagccac acttccgtct
      301 tcatgctcaa ttgtgacata atttcctgac aaagtatgat acgaagcttc tatgaccgtt
      361 cctgccttgc tcgcatagat tggttctcct tgattggctg ccatgtctaa tcctctatga
      421 aattcttctc ctcgccaacc gaaggaacta gaaattgaat aattttttag aggtaccata
      481 tagccttctt tgcttagttg tggaccagct acttctttat catatttgat taagtcatag
      541 gtttcgatta acccatttaa ttttttatcg tagctagtgt ctgttgcgta tcttcctgtt
      601 aaaaatttag tcgcttcctg ataggttttt gccttacttt tccaaacacc atcataaaaa
      661 gaggtatttc caactaagcc ttcttttaac agtttggcat agtcttttaa gctttcttca
      721 tagctctcat attgccgaaa agttgcttgg gtcgtataaa gtgtcccatt gcctaaatct
      781 tcttgtgtcg caaaagagac gccctttccg ttatgtgtgc ctttgattcc aaatagattg
      841 tagtttggtg cctgagccaa ttgactttgt ccgcttgcgg attctaaaat ggcttgcgcg
      901 atcataacag aagcgtataa atcattttct tgtccaattt tacgagcaga ttcaccgatt
      961 ttacggataa acgtttctac agacgcgtct ttttcaaaat gaatagtccc ttcgttatct
     1021 tgtgtgctgg aagggactaa ttcttgtatc ggtgcgcttg gtgcttggtg gtagtttttt
     1081 gtctcttctg aaccgattga ttgtgcctgc ggtacgtcat tcataggtac agtcacactc
     1141 tgttcaggtg ccttttgtgg gcgttccgtt tttgttggcg gttccgcttc tgtttcggta
     1201 cttggttttt gaggagtctg tgagctttca tcagaaggtt gctcatttgg ttcttctgac
     1261 ggttcctcct caggttgttg attgtcttct ggtttgtctg gttgattcgg tgtttctggt
     1321 tgttcttcag aacctgttgg tggagcgttc atttctggat tatctggtgt atctattgta
     1381 tcttcagtcg gtgtttctgg ttgattttct tctccagtgg aatttgttgt cgtttcttca
     1441 gtgatcgttg cagattgcga atttgtcagt tgattgttgc cttgttcatt agccaaaacc
     1501 gctggcgtat acccaaatac ttgacctact aataacaaag cagataaaca tttactcgtt
     1561 tttttcacaa gattgcctcc attcttcaat caatcatcta gagacagctg attgctgtct
     1621 ctagtcatac tttaattgct ttgtttcatt ttacgacgaa ccagaacagc ccccacacca
     1681 acaaaaagaa acagtcccaa tgcaattaac acatcgttca ctgtttctcc tgtttttggt
     1741 aagctttgtt ttggtgtgct cgtcggcgtt tgtttgacag gggttgtcgg cgtttctttt
     1801 ttaatgcctt ttgctgtcaa tgtttgtttt tcattcttca aatcaaagtg acgtgcttct
     1861 tcctctttat ggtctttatc cttatagaca atatgagttg cgaccaattt ctccccgtct
     1921 tttaacgtgc ctgctttgta gtcaaaggat actttaaact cttcagacgc tttagaaact
     1981 ttacgtgcag tttctaccgt accaacgact gtatcttttc catttttatc aatcttatgg
     2041 aattgagtca cccagtattt ggtttcccct accgtagatt ctggaaattc ttgcgataca
     2101 tgatcgatca actcttggtc tttgtctgga tcaaatgttt ggtcgccatt cactgtaata
     2161 aagcgtgttt cgatttttgg ttctttcgca gtcactgttt gatctttatt ggttaaatca
     2221 aaatgacgag cgaattcttc ttcctcttct ttgtcgttat agacaatgtg cgttgcgaca
     2281 atcgtgtcac cgtctgaaac catgtctgct ttgtagttga acgtcacatc aaatgtttct
     2341 ttttcatcgg tcacttttcg tgtttctttt accgtatcta atacagtgac ttttccatcg
     2401 gcagtaattt tatggaattg cgtcacccaa tatttctctg tcccaaccgc agatttaggg
     2461 aatacttggg acactttatc cactaattct tggtcttttt tcgggttgaa atcttttcca
     2521 cctgtcgttg tcacaaacaa agtagaaatt tctggttggt cttcttctgg aaccacgatt
     2581 tcttcgtttt tcacagtaat tggttgtact tctccgtctt tgaccgttac taaaatcgtt
     2641 tctgtttgtc ctgacgcttg cccataatgc tcagggaaca caatttccgt aaatttatac
     2701 gtaccgtctg gtaattttag tttttctggt tcttcttttg tttcatacac aaaggacaca
     2761 tttttattat gtgcagtcat accttcaatc aggaatttcg ctcctggtaa ttgtagatga
     2821 ttgattgaat cgactttatt gaagtctact tcacccgttt ttaacaagtt gtcaatcaca
     2881 tctaattcaa acgtaagcac aggcgtcgtg ttatcgccat aggccacaga gaacgtggtt
     2941 tgttcttcag aaggttgacg gtagttgtct ggcacatttt tttcaatcaa ttggtatttg
     3001 ttttgatcgc cattgtagaa ttttttgttt gccttccagt gtccgtcttt gtcagttttt
     3061 actgaatcca agacttctcc ttttttcaca aggactgttt ctttatctgg cgcagtaata
     3121 tctgttttgg cgataatatc aaattcgata ttttctgcag gaacggtatc ataaacaggt
     3181 ttgccttgat tccaggattc ttccgcatta tagacttctt tgactttctt tccttctgga
     3241 atgaactgtt gtaagtcatt ttttgcagtt aacgtcactt ctttgtttgg ttcaatcgta
     3301 atcgtatgag tttgtttgtc tagaatatac ccagctggtg ctttgacttc tttgacatcc
     3361 aatttatttt gaacatattt tccgtctttt tcatagaatg cagggatttc ttttaaggcg
     3421 atttcccctt tggcatccgt tgtatattgt tttgtttctc ccgtatcatg acgtttgatt
     3481 tcatacgtca catttggaac ttttttccct gtgacagcgt ctagtttggt tagtttgaag
     3541 tcgccatttt ttagaacttt aatagttagt ccagctctat taggatctgg cacataaagc
     3601 tttcctagat tttgagcaac aggatctaca taggcaaaac ttgaacctac ccctctagaa
     3661 tttggatcca gtgaagaaac agaaattcgt ccgtcatttg agttttgatt tgcttttata
     3721 aacaaactat ttcctgattt tcttacttga acgttagctg tgttttcaac aaccgaccca
     3781 tcccattgag aaaacacacc ttttgtatcc ttaacttcta cttcttcccc tactttaacc
     3841 gtcactgttt ggctatcaaa agaaggatct tcaagtaaat tgtttatttt tctatctaca
     3901 gtctgtttga aagcttcata attagattgt ccaatagttc cataattaac aaatgaacca
     3961 cctaaaactt cccaaatata tgtttgtgct aatacatagt tatccattga accactagaa
     4021 tagtagccaa aataaccaat taaagaaatc ttttttctaa tagcacttgt cgtaattaaa
     4081 ttttcgagtg gtgtcacatc atattgaata cccgcactcg tgattgcgcc ttcttgaatg
     4141 caaaaaacat cttctccgtc aatttgccag cgaaccatgt aatcatgagt atacattgtt
     4201 ccttcaggag taattcgagt catattcaca tctttacgac cattttcagt caatgttaga
     4261 ttggtagcac gtgtctgtgc attcttttcc aagattgctt gccattcttc agattcttgc
     4321 gccaatcgtt cttgttcaga aaaagaagtc gctttttgaa tatcttcaat ggttataaag
     4381 tatttctcat aaccttcagg gacctcttga gtcattacat tgggcggaga aacatcttct
     4441 gtcgtgtcct gcgtagttgt ctcttctgtt gtttcacttg tggtttcagt actgatcgct
     4501 tcttcactac tttttgattc ttcagtcttc attacttctt cagtagaatt tgattcattt
     4561 atcgtttctg caaatgccgt tacagatcct aacatgggat tgagaaatgt aacgaaaatt
     4621 gccaaccaag ttgccattct tttaaacata tttttttcgt tcctttcatt ttatttttca
     4681 gtcttctaaa gtaatttctt atggacttcg acaccgaaac atttttatcc tcctcaaaac
     4741 aaaaaaagac tagaacttat aattctagtc ttcatcaacc acttactttt cttttatttt
     4801 tagtagaaat ctacagtgtg tttttgtgac ccgtcactgt aaataataac atccaccata
     4861 aaacctttgt tgtaccactg cgaatttgga tcgctaacct ctgcattagc ccaatcatag
     4921 gcttcatcta cagtgttaaa tacctttcca ctattaccaa tctctccaac tacaattgtt
     4981 ggctcttcag gtgtcgttgt gccaccattt ccgccttgat cgcctccacc aggattgctt
     5041 ggttcttcag gcgttgttgt tcctccacct gtgcttgggt tagaaggagt tgtttctcct
     5101 ccagtatttc caccattacc tggttgtgta ggtgtttgag tagtgccacc attgttttct
     5161 ggtgtacttg gcgttgttgt accaccagtt gttcctgttt gatctccagc tggtgttgtt
     5221 ggtgtcgttg tatttggtgt cgtttgtgta tttgaagtcg ttccttgtcc tactggttga
     5281 ttcggatcaa ctttgtcttt ctcttgaatc actgtttgag ccacagtttc acctgcgctg
     5341 ttttgtacac ttgctacatt gccattaaat actaatttat ttcctgcata gattaaatcc
     5401 acatttgtaa tcttattcat gtccgctaat ttttggacag taatatttgt tgcttgactg
     5461 attgcgctta gggtatctcc ccagacaatt gtgtattcat tccctttaat ttctgctttg
     5521 atttgttccg ccgaacgagg tgtccaaact ttgttttctg ctgcttcagc attcgttccc
     5581 acagcaagac taactcctcc gactacgatt cctagtgcca atactttcaa cattttcatt
     5641 gtttttcgcc tccgatttga ataaatttat ttacaaaaac atagtaccaa acttatgtct
     5701 atcagtcgat atattttttg tagatttttt tattttttga tatactaact ttggtactaa
     5761 aataactaaa aggaatgtca cacatgcaac ttaataattt aaatgaaata catgattggg
     5821 ctcaaaataa tttacttaat cgtaaacaag ctgccaaaat cactggacaa aactatgatg
     5881 ctttttctag ctctttacgt agaaataata tcaaaccatt tctagaaata ggcgacgaac
     5941 ctggtccttc catcattcgc ttatatctaa gagaagatat tgaaaaatac gcaaaacagc
     6001 tcgaagaaaa aagacagaaa cgtcaatgag gcgttttctg tcttggattt acaagtaaaa
     6061 caataataaa cacttttaca acggaggttc tatgaataag aaatacattg tttcagttgg
     6121 ttcaatcgta ctgattgtac tcgctattat tgctttttct gtatccaaca aaacagaaac
     6181 acagccacaa aatacggttc aatcgactac tacaagtcaa tctagcgatc attccgtcta
     6241 tccttcacta aatagtttaa aagaaacgca tggacaagat ttaattgatc ttggttcttc
     6301 tcaaattact actgtcttat ttgaaaagaa aaatattgcg tttattactc ctaaagaagg
     6361 aactgacctt gaattttcta tcaaagtaac aaataccaaa gaaaatttat tctccattcc
     6421 tgacattcaa tataccccta acaagttaaa gattggggat acgtttggtt tggctaaagt
     6481 aaatgaacaa tactttgctt actcactgaa ataagtttct aatagcaaga ggaaaaaccc
     6541 tcttgttatt tttatcacat aatcatttta taaaaacgct tcgaaaatat caacattttt
     6601 gaagaaacta tttttctaat ttctcaattt cttccaaggg attttcgcta atgacccata
     6661 caggcacaga ttgctctcga ggctcccctt cttctaacca ccaacctgca taatgaactt
     6721 cttcctcctc agcattacca tgataccaac caacaaagga ttcatctaca acgaatgaaa
     6781 tttcttcttc tggatcaaat aattcatttt tccatgcttc aattgcttgt tctttggtgt
     6841 acttttgttt gttaaataca ccacaaattt cgtcatctac ataaaaaatt tcaaaatcaa
     6901 aattactcgt tccttttact ggcatcttaa cttttcctcc tttgactcac ttcaatagat
     6961 ataccactca tgttcattcc attgtttact agttaattgg agttttatct tagggctttg
     7021 tgcactcttt ccaaaaactt gagcgtcgtt tttttcttct aaccaaaaaa ggtaatttct
     7081 tacattcggt actaaatgtt tttcctgcat ttcttcatga aaaacatata attctgcttc
     7141 atttttcaac caattcatta ttcattccct cattcacttc ttcataccgc cattcagata
     7201 gtgagatttc tttttgtttc tccacttgtc ctataaaaat cgctgtattc aacaagctga
     7261 tcacacttaa taataaagca atgaaactaa tggttacact tactctttca cattttgtca
     7321 ttttgtttcc ttctttactc gtttgttatt tcttgtaata ggtctaataa ctccttcacc
     7381 gaaatttgca aatggttcgc aaaaatttca ataaaagcac tcgcacacat taccagacct
     7441 tcaactgata tatcactgcg acaataaaat tctggagaaa cactgatttc tgattctttc
     7501 ttacaattga acgaaaaaac tatttttgaa tcaaattccg gtaattcttc cacaactatg
     7561 ttttgggatt ctaccaagaa agggagtgcc tgttctaatg cttcttctgg tgtttgagca
     7621 gatagaataa ttgcttcttg ttcttttatt tcattgttct caaatacttt ataacttttt
     7681 ttcattgtta gttcctcttt cattcctctc tcaattagct aagccgttac tggtacgcca
     7741 ttctctaaat ttagtatcgc taatccttaa tgctcgtcga atatcaatca ttcgataacc
     7801 tattgcacga agttctttat atcttttagc agttagcgtc tctaacgtca tttcaggcaa
     7861 tgggttcgaa tcaaaaatag aattcgaacc gattatttgg gccaattgtt gtacttgtaa
     7921 cagctcttct gaacttaatt cagtgattgc tcgattgcct atcattgcat acattttctg
     7981 tcgaatatac ttcttttctt gtttagacaa catttctatc cactcctatt ttcattatca
     8041 tttgttacct ccacttcagt aaatccacaa ataaatgaat tcatattcca agtaccattc
     8101 ccttcaacta caacttttct cctgtcctct ttcggatatt caagaatcag accgtttacc
     8161 aatatgtttt tcactactac taatttgttt gtataccgtg gaattgctgt tccgatatac
     8221 ttcactttat ttcctacgct aattgtcatt tcctcactcc tattcttttt tatccaaaac
     8281 tttttcctgt cctattcctg ccaacgaata tacttcactg catgcttaaa caaaacaaaa
     8341 tgttgccctg ttttatttcc gtcttttact tgttccacat acagttcata tttggtcaca
     8401 ccttttaaaa cgccagatac tttttttcat ccattgtcac aattagtttt ttcatcaatc
     8461 gattgatact gcactgctca tttttcttgt tcatatagta ttcctttctt tttcaaaact
     8521 agcctctttt gtttactagt tttcatcaat catcaacgat ttaacagatt ttttctttcg
     8581 cagggtttct agcgtcagca accccaaact ctaaaaaagt gcctttgctc ccttgaaaaa
     8641 cgagcggacg attgtactcg tcatcatatg tttttcatac tacctgatcc acgcaaataa
     8701 cctttttttg ttctgtgcca cacccatcca tacgtatcgc ttgagattca ccatactttt
     8761 ttaggtggtt tatccttcct aagtccctgt tagacacgct gattaactta gaaagtcctg
     8821 tttgtatcca attttcaaag atctcccaaa agaaataagt aggtttacgt gtatcgcttc
     8881 tgttcaaagt acctgtttgt acttgagtat ttctactccc ctcgttcaag ggattccttc
     8941 caccagctag cttttccttt catcatcaaa tgttcctgaa aagcatttac gtttcgtgtc
     9001 cgtgtgctta cttattcttt tttcgatagc gattatccgc tcgctactcg ctattttata
     9061 ctttgtttgt tagctcaaat aaaaactaac aaacaaaaaa ggctagtttc tttccgtttt
     9121 atttttttaa ctgtcgataa atagttgtta gtttaattcc aagttctaaa accaatacgc
     9181 ccaacaaaac cacaggaaaa acaattgggg caaacaagta aaatcggata gttttcatgt
     9241 cttccctctt tctttttggt gtcgctgtgg tcgttcttgc cgttcaatac ttcgatacat
     9301 tttttattca ttatttgctc gttttgtttt cctctggact gcttttcttg ctttttgaat
     9361 actttatacg cttttcgtgc ctgaattaat aggtcttgtc ggcgtttcat tcgtgagaaa
     9421 acaatatttg aaaccatcaa atcctggttc gaacaaccac gattcgcaat aattttccca
     9481 tcaaaaaaaa tagttttcac ttgagaaaaa aacagttgga aatcttgttt tgttgctaat
     9541 ttttttgtca aaatcactgt gcttttccct gttgaatcta caatgattct tcgaacaatc
     9601 cgattcgata acttccaacg ttgccgatta aacaatgtta cttcacaaca aatatgacgt
     9661 aagtgattag gggtcatttt catgtgtcca attcctccaa ccaattatgc atcgaaattt
     9721 tgggcaattg tgacagccca taaatctgcg tttccaggtc ttcataacta caagaacgct
     9781 tggcttgaag taatgaatat tctgcttgat ctgatagttt tgttgccatt ggatctagat
     9841 tcaattctct tacacaacat tcccaaaaat tcaatagcgc tctaaaataa aatccttttc
     9901 gattctgaat tgcttttcct tcatgacgag caattttttc attttgaata caccgttgat
     9961 acgttttgct taaatcttct gcaaatgttt cccctaaaat tcgatactcg cttccatcag
    10021 ataaaccatg tgtttgatta tactttcgtt gccgcttttc tataattcgt ttactgtcgt
    10081 atatcatgtt tagctgctct ttcgcttcac gccattcagc tgaactgcga acaatccatg
    10141 tcaaaacatc tttcggaaca aacgtacttt cgctgtaatg agcaataaac tctttttgcc
    10201 aatcatcagc catttcaaca ggactagtcg tatcgatttt tgtatctaga ttttctgtat
    10261 ctagattgtt agaagaatta ttaaataatt gtgcagaatt ttcacatgga tttttttcgt
    10321 caaaatcatc gaaaatcgct aaattatcaa cagattcctc ctcttctttt ccttgtgcag
    10381 aatctacact ggttgagtca acaggaccta atgaatacac atcattttct gtcgtatcta
    10441 attctcctag atacaaccga gctttgtttc tttttcctgt ttgttgatca aatcccatat
    10501 cctctatttc tagtagctca tattctttca attctttttt gattgtatta acagaacgtc
    10561 tgcaacaatt caatgcattg gctaattctt gttccgtaaa ttcaaaataa acaaaacctt
    10621 gactatctac ttgatttcgt tgaatggcta aatctaagcg tgaccgaaaa accatatacg
    10681 cgatttttgc tttatcagac aatgagcgat atttttcact tgtaataaat acctttggaa
    10741 attgatagta taattcattg tatttacgag ttgcggtaaa tctttttgtt gccatcgtta
    10801 ttacacctca tcttgtccat atattaagaa agaattttgg tgttggtaga cacgaacatc
    10861 ttccattgta gcaatcaatt cttttagata aattcgaccg aattcaattg ttagtaagtc
    10921 gtaatcttct aattcatttt tgatttcttc aatttcttct tcagacacat tgagtaaaat
    10981 ggctaattct tgattggtat aagaaatata gacatgtttc ttttcatcaa tccgtttttc
    11041 aattaaggat tcttctaagt actcattcag taagctatag gcgtattttg cttgtgcagt
    11101 catcgcataa tattttgacg attttataaa gaatagtttt ggcatatgaa aagttgcttg
    11161 tgtcagcaca tctgttgcgc gaaatttgtt ttttaccata ttttttcctc ctagaaaaac
    11221 acgcaaaaaa agctctgtat aaatcgaatt acacagagtt tcttcttgtt gatatttaat
    11281 ttctaaaata cgtttcgggt aaaaactaaa cacttactaa tatttagatt gtagaatctt
    11341 cgccttcatt tattcatttt aaagagaaaa agatcaagtg aaatgtgccc aaaagtttga
    11401 ttctattaat ttttaaacac cattcataat atatttccta taatgtaaaa agcgtcgata
    11461 cttattaaag catcgacgtt atttttatag taaatttatc aaaatttttg ccaacatttt
    11521 ttccgatttt tttccctatt tctatctttc cttatctttc ttagttaata ttatttgatg
    11581 ttaatatatc actactaata ttaaaaaaca ttgatttatc ataggataaa gagtgatagt
    11641 ttaatactta aagagacata ttaattttat ttaaaaattg acattaatga ctcttaatca
    11701 gcgggtcgcg ggttcgagcc cctcacggcc cattgggtgc caaacccacg gaacaggtaa
    11761 atgttggtgc gaaagcattt cgaaaacatt tacgctgttc tttttttatt tttcaaaata
    11821 actagggctt tttattttta actcaaagtc cactgtagcc ctttggactt tgagttaaaa
    11881 aagaagcata attatgaaca gtaaagtttc taatataaca ataattcgag aagctttgca
    11941 aaatcatgct gccaatgaac tctctaaaca tataggatta aatctaagta ctataaaagg
    12001 ttaaaaagtg aagaatattt aatagaaaaa ctaaatttac ttgctgcttt ccccttttcc
    12061 attaatacaa caatcagaga gtgtcttttt ttcgaaaaaa ttagaataaa taaaagacac
    12121 gtagtttttt tatctacatg tcttccatcg actacaagtt atcatttctc aaaacgtatt
    12181 tagcttttaa ttgattaatc gtttcacgtt gtctacacat tgcctaatta agattttctt
    12241 attagaagca tcaatcccgc aataatacct aaaactccag gaattaccca caataatgtt
    12301 aaaagaaaaa atggaataat agataaaatg agtaaactaa taccatagac cactgttttt
    12361 tgattttttc ttactaaaaa acctattatg aaaatcatta tcgaaaaaac tagggtaatt
    12421 cctccaaata aaacaacatt cccgcttaga ttgactaaca tatctatatc cttactggtt
    12481 agttctgtag tatttaaagt agttgaatca atagaagata gaaacatacc acccatcaat
    12541 gctaaaagac tacataaaat ccctgttatt ccactaatta aaatcaaaat aaactctgtt
    12601 tttctcttat tcatttaatt ctctccagat taagtgtttt agaattagaa attttttgac
    12661 catagttaat agcttctaat gaacgaataa caaccactga cgtgttagaa aaataatccc
    12721 caatatgttg tttcttgggc gtgaaagcag ccaccaaata cccaatttga aatacaaaat
    12781 tttttaatat aaacctacat atacactcac gaataaagat agtttgccaa cttagctctt
    12841 tttcctcaaa gctcactact cttatcccaa aaatcatttt cccaattgtt tgtcctgaat
    12901 tcagcttagt caaaagtaca aaataggtta aataaatagc taatgacgct ataccataaa
    12961 aagtaataat ggatttgtca gattccagtc ccatgatttt gtaaaagaga cctatagtag
    13021 catgggtgat gatgctaata caaattaaat caataagaaa agcaaaaaat cgtaaccaaa
    13081 atcccgcaaa tacataatta ggcaaagttt caatgccact cttcttattc attttatagc
    13141 cctccatagt aatacattgg ttttggagta tcagaaaatc caagtttttc aaaaactgaa
    13201 attacacgat ctgatgtagt tgcctttagc ccttgccatt cagcaatctt attacctaac
    13261 caagaagaag caaagccagt tgtgtcattt ttatattcta taaccgtcgc atcagttaat
    13321 ttcttctctt tttttaggga atctaatgca tcttcagagt aaccaatttg atccaccaac
    13381 ccattttcta ctgcttggtt accatcataa attcttccat ccgcaagttc tttcactgca
    13441 ttagtatcca tatttcttcc ttttgctaca acattgacaa aacgatcata agcactcatc
    13501 acaaattcct gtataacttt attctcttcc tctgaaggtt tgtgttgagg tgacatcata
    13561 tcttttaatg caccactttt atacgtcgca tcagttacgc caagtttttc taataagcca
    13621 gaataattta aaccagaaat aatgacacca attgatccag ttgttgtttc ttccgtagca
    13681 aatattttat ctgaaccagc agaaatgtag taccctccac ttgccgctgt atttttaaat
    13741 gcagtataga taggaatatc tagcttttta atttttgcca tttctttagc aatctcagca
    13801 ctttcatata ttcctccccc aggtgaattt acttctaata acactccctt aaccgctttg
    13861 tcttcttgaa ttttctttag ctgtgttaaa aaattctgat ggttatattg ttccctacta
    13921 aaaagattgc tttcacctgt atctgcaata actccattga cggatagttt gacaatcttt
    13981 ttatttgatg cgccttcttc taacgtttct tctgtgattt cgttagatcc gtaaaaaatc
    14041 ttatttattc ctgttagggt tgtatttgat gcttcctctt tatcttttcc tggaattagc
    14101 gcgacgataa tggatagtaa caataatccg cacgctactc ccacagcaat ccatcttctt
    14161 ttattcatat aaactcctcc tatattctta gtaaaaataa ttatttttta tttagaaaca
    14221 catcgacaat ttgttgccaa tatgtttttt ctcctacaat taaagacaac tctccagtta
    14281 atccataacg aaacggaaac gattgagttg tttttaacgt tcctttcact gcataaaaaa
    14341 cacctttctc agtggcagta gaattttctg aaatttcatc taaagttccg tcgatcgtaa
    14401 tcggagcccc acctttttga tccaatttaa aatgaacctt catcccttct ttaatatagg
    14461 tactttcatt cgcaggtaaa agtgctgtaa atttcatttt tgatttttcg tggatcggat
    14521 aaatttctgc aagcactgtt cctttggcta gttctgcttg tccctttgtt tcttcattta
    14581 aatgaaccgt tccagataca ggagctttta gtattccttt tgaaagttga tcgtccaaac
    14641 ttttgataac tgactggttc ttctcttgtt tttcatttag ctctgtaact gtttgtttta
    14701 cagttgttat tgcttgttct agctcttgct ttcctttttc cgtttgacta attctgtcat
    14761 tctcagaagt agttggttta actaatttag ctttttctga ctgatactgt tctacttctt
    14821 tttttagttg ctcaatttgt tcatttatgc ttgctgaaat tgtcagtttc acttgatttt
    14881 tttgatcatc agaaacatta tttagttgtt cttgccaatt ctcatagttt gccatcattt
    14941 cttttgaaaa atcttttaat gattgattat tactccaagc tacctgtact tgttgccatt
    15001 cttgtaattt actatctgaa ctctcaattt gtttttctag ttgtcttttt gtcttttcgt
    15061 atacttctaa ttgcttttga tcatttaatt cactttgctt caaagcgtag cggagtgatt
    15121 ctttttctga caacatgctc tttaattgat tactgtagcc aaaactgtca tccgttgaaa
    15181 acaaattttt tcctttactt atactttcaa taaatagctg agccatcttt atttgttgag
    15241 taatacgttg gttttcttgt tctatctgag ccttttcatt ctctattaaa gaacaatcca
    15301 atctaaccaa cacatcgtcc ttatgaacaa attgattttc ttttaggaag ttttccatta
    15361 ttttagcttc taatggcact tgtaacttct ctattttttc ggggttcaat tgagccgttg
    15421 tccgaataac aacctctttt ttagcaaagg ttaaaaacaa tccaataata cataaaaaaa
    15481 gaattacagg gtataacacc caacggtaga agctatgatg ttgtttagta tagatactag
    15541 aatgttcgaa ccaattatta ttctgcataa attgtttccc tctcctagat ttcccataat
    15601 ttttgataga taccatcggc aaaacgaagt tcatcgtgcg ttcctttttc taccaaatgc
    15661 ccttcatgga gaactaaaat ttgatcacat gcttttgcta ttggtaaatg atgcgcaata
    15721 aaaataatcg ttttttcttt taaattgagg agataattca taattgcatg ttctaacaag
    15781 gtatctaatc cactagttgc ttcatctaaa attaatatgt ccggattttt taataaagcc
    15841 cgtgctaagg ctaatcgttg tcgttgccct cctgaaagat tatttccacc ttcttccaac
    15901 aatgtctcgt aacgtaatgg ctgttgatta ataaattcat ttagttggac tatctcacaa
    15961 acttctatta tttgttcgaa acttggtggg ctatctaaac cgaatataag attctcaatg
    16021 attgttccac taaagaaaaa tgactcttga ggcaaatatg tcactcgatc gcgtaatatc
    16081 tgaaaaggaa tgtctaagtg attaatgttt ccataatgga tcgatccttc tgatgcttca
    16141 taaaaacgaa caaataactt tgcaagagtt gattttccag aaccactaac tccaactata
    16201 gccgttttac tgtgcgaagg aattgtacat gaaatttcgt tcaacgttgg ggccttcatg
    16261 ctatacgaga atgaaacgtt ttttacagtg atatcttgtt gaaataattg ttcagaaagc
    16321 atatattcaa aagactggtt cgatttttct gaatcaatcg cgaaaatttc atttaaacga
    16381 ttatttgcaa cttgagccgt ttgcattttc acttgtaaat taataatgtt ttgtaaagga
    16441 tctgtcaaaa atactagtaa agcattgtat gtgataagtt gacctagact aattctatta
    16501 tccataacaa aataagaacc tagccataaa attaatgcac tgctaattaa ttgaatcaaa
    16561 tgtttaattc cttgttgaat attatcaagc gtaaccgtac gaaatgattt tttcattaat
    16621 ttaacaaatt catggtctat acggttatat actttctctt caccgctata tgctttaatt
    16681 gtttctattc ctttaagact ttctatgata ctagaattta cagctgcacc ggctgccatt
    16741 tcttccctgt tcgatttttc atagcttttc acgaaagcga atattgcaac tacataaaaa
    16801 ggcaaagagc atagagtgat taaaaacaac gtactattct gaatagctaa cgtaacccca
    16861 acaagcaaca ccataccaat atctaaaaat attgataacg tagcacttgc taaagcatca
    16921 ataatcttac tggcatcaag aaaacgagaa ataatttccc ctgattttct cgtagcaaaa
    16981 aaattcattg gtaaagttaa cacatgtttg aaatactgaa gcataattgc catactcatt
    17041 cgctgtccta aaataataag aagataacta cgactatatt caaacaatac ccgaaataaa
    17101 tagacagcga tcaagccaat ggaaattata tttaaagttg aacgtgcttg attagggatg
    17161 aaaaaatcaa gaataccttg aaaataatat gagcttccta tgccaaaaaa cgtaatgaaa
    17221 atcgatgcta aaacgatgtg gaatactaat cctttttgtt tccaaattat aggcaaaaaa
    17281 gaacttaatc cagctacttt ttctttaata ggaatataag tttctctagg cataggtaat
    17341 aaaagaatac ctgtccattc tttttgaaac gcttcaatcg tttttctatt ttttccatta
    17401 gcgggatctg caatccatag tacgtttccc ttcgttttat aaattacaac gtagtgcata
    17461 tagctgttat caaccaatat atgtgcaatt aatggtaaga taagatctct ttctttccag
    17521 atctcactat ctgcttgaat agccatacaa tcaaaagaca atttttcaaa tgtctttttc
    17581 aatccaaatg ctgaagtacc ctctaaatca gtcccagata ggtcccgtaa tttatgaatc
    17641 gggatatcag ttttaaaata ttttaaaatc atcgaaaggc aagcaacacc acaatctttt
    17701 tcatcttgtt gctgcacaaa aggaaatgat tttatcactt atctaacaca actccttcta
    17761 aaaaattttt atagaaaaat tctcaatata gctttaatac agtgaaaata ttgtctcata
    17821 attccacaaa aactttgaaa acagttcttg aacactaact ttttcattcc tgaatcaaat
    17881 ttattttgga aattttacta ttcaagaata aacttgataa gtttcaggaa gatttttact
    17941 gttaatcaaa tacgtatgtt acagtagcac cgtaaaaaat attatttaaa gaagcgtgtt
    18001 taaacatgca aaatgtaaag gaactaagta ttgtggaaat gcacaaactg ttggtggagg
    18061 caataatgct tggggaaaat tggggcaagt tgtaggtgga cttaccacag gggcagtagg
    18121 tggtgcaggt ttaggaacag caatttgcgg accagcttgc ggtgttgttg gagggttata
    18181 tggagctgtc gctggaggtg ctgcagctgg ctgggatgct cgaaaaaaat aaaaaaggag
    18241 aataagtagt atgcatatta aaaacacaaa aacaacattt atattatctt cagaagagtt
    18301 gaaaaatatt caaggtggga gtgccgtggg agttttaggc actacttttt ctggggcaac
    18361 agcaggagta aaactttgtt cagccggagg accttatgca atagctgctt gtggtgtagg
    18421 tggagcttta ttaggtgctg gattcagcat gtggaccggt gcgtaaacaa ttaaaaaata
    18481 tcaaacctca agattggttt gtgtcgatta cgaacatagg tatgttcttt atcttttcaa
    18541 aagtttggaa tggacagaac tttttactaa agcttcttgt gctagttttt ataattatat
    18601 taaattttta tcttttatta aagtcagata aaaattcttt ggaaatgtaa tggtagagat
    18661 atttttacaa aaggacttgc agcgctgtag ttggtgctgc aggaggacct atttcggccg
    18721 ttacaggagg tattggtaat gtacttgtcg gatgtgcatt aaagcctgtt cctgcaagtt
    18781 aaatttataa aaacaggaaa gtaattatct caaatcctat caagtacaat cataggattg
    18841 cacttgacag gacaaatttt ttaactaaaa aatgaaaaac actttctaaa aattagtaga
    18901 atttgccaaa tctttcgcat cttccaccat acatttaatt ttaattcccc tttatggtac
    18961 ttaataaaaa caagaaaaga actattcatt tggccctttt ttggttccag accatagaaa
    19021 tattcctcca gtaactaata ttgctgttat gcacgtaact aataacagct ataaaaattg
    19081 gtacaacttt aataggattc agaaaaattt gcactagtga aggaatctat tttattagaa
    19141 aaatgcaatc tccctaaaga aaaaaccact aaacagtgct ttctttcaac ttctccttaa
    19201 ttttttgtgc tttccctaaa gtaaagtttc tggccaactc tgaatcaaaa tatagtgaac
    19261 gttttttaaa atctatttct ttaacgttaa agatatttac taaacacgct cgactaattc
    19321 ttgttaacat aggatatttc ttttcaagct cactaattct tccataaaat tcgtactgcc
    19381 catctttagt acacaatgat aatctatgtg gtaattttga agcttctaca aaataaatgt
    19441 catttatatc aaaagtaaat gtttgagaac ctatggaaaa aacaaatgct tgattcgtaa
    19501 cctgttttgt tgcatctatc ctttcttgcg ctaataacaa taactcaaca atctcgtttc
    19561 gatactcatc tagtgtttga tcttttgtta caaatcctaa cgtttcaact cttcttttta
    19621 ttgttacggg taacatctca tcatgagtag ttgtaaaaat gatttttgct tgaacatcat
    19681 attttctgat tacttctgct aattctatac cgttaacttc atgatttaaa tcaatatcta
    19741 agaaatatat tccttgcttt ggtcggaatt gtttgaggta ttttttaact tctaacggac
    19801 tttgtgtttt caatactatc ttaaatactt tatcgtgaaa aagaatgaaa ttatcaataa
    19861 ttcgttctaa ctgatttaat tgtataattt gatcttcaca taatattatt ggataactca
    19921 taaatcctcc ttccctcaaa acaacaaaat aacttgagta gtaaattgat acggatcgtt
    19981 ttgatagttc acaaacatat tgggattctt tttattgatt tcctctatac tagataaacc
    20041 taacccttga tgtccctttt ttgttgttat tccttttttc attagagtat ctatagagag
    20101 tagtatctct ctacaactat tcgatatcga aatttctatc tgtttatcat ctcggtaaat
    20161 agctagagac agttctctat cttgactctc tatagatgct tcaattgcat tatcgagaat
    20221 aattccaagc accctaatac agtcaaatat tggaacagga acttcaataa tagtacttgg
    20281 acattcgaat ttacaacaaa tcttattgtt atttgcttgg tatagcttcg ctatcaacaa
    20341 acttttcaaa tattcatttt ttatattttt taaataacga tattcaaact tattggttag
    20401 ataagaatta gaatagatct ctaattcttt tatttctttt tccaatatcg aatcatggtt
    20461 ttgattagcg atttctttta aactcagcaa taaattttta tagtcatgtc taaatctggc
    20521 aaatttttct tgatcttttt ccagtgaatc agtatatttt tttagataaa ccaattcttt
    20581 tcttttcaat tgctgttcat attgttctcg ttgcctaata gttattctaa taaataaaaa
    20641 tagaacaaca aacatttgaa tgattaaaaa tattaagaca ccaaacacaa aatgatcaaa
    20701 tacttgatat tgatgagcaa cataagaaac aaataaacca attgtaagta aataaatcat
    20761 gaatatagca gtaagtttag aactggactg ttcaacaatc ctatttatag ctaattttct
    20821 atataaccaa ataaacatca gcaaaaacaa agaatcaagc attatctgga atactacata
    20881 accataccct tttatccctt caaatgaaaa catatacgtc ataaccatgg aagtaataat
    20941 agatctgaaa tcatcaataa gcatacataa tagtaattta tttaaaagca ctatattagt
    21001 tttcttcttt tcctttaata taaaataact tccgattata ggtatagctg cggaaacatc
    21061 agtaaataat tcaacaaaaa aagtgagtgg tatcaataaa catacaaaaa gatattttct
    21121 tttgttaaga tcagtattag tcatataaaa taagagaaaa aagaaaaagt ttcccacgaa
    21181 cattcttaat agataagctt ttgtatcgat agtaatcaag agatcccctc ctgattttag
    21241 gatatcataa aaaaaatttt agttacactt tttaaataat gaaaaaacac attccactaa
    21301 atttgatgcc ggttttcctt gaggttttgt tccagcacct cctttaattt ttttaagctc
    21361 ttgatctgaa cattgtttag catttagtct atttttttct tccattacga ttccccctac
    21421 aaactataaa tacttttatg tcactgattc aatataccta tttttctaat caaaaaattt
    21481 tgagacatat ttaatattat aagtatcatt aaaattgaga tttatctcca taatctgctc
    21541 gatataaacc atttattcca gcagttcttc caatttcact caaatttctt attaattcct
    21601 cttgatattc agtgaatctt attttattag tataagcagt aaaatataga taattcacca
    21661 atctatttat taaaggaccg ggatctaatt catatttttg tttttctaat tttaaatata
    21721 ccttttgtaa aacctcaaga atatcagaat atttaggatc tttacttata gatatatcat
    21781 tatataattc atgaacaatt tgcttagcat tttttttcat tttagcactt ccctggaatt
    21841 gctccaccta aaaaaccacc tatagacatt cctgcaatac aagtagttgc cttggcccaa
    21901 tcgaccgtac atttattttt agtgcaatac actccatttc cataatattt tccactatga
    21961 gtggtacccc catagataag ttgtgtctct ttaatagaca aaatttttaa atgtttcata
    22021 atctaatcac ccctaaatta atgtttttag actattattc taacattcat taaattt
//
LOCUS       DACDTH010000044        22044 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDTH010000044 DACDTH010000000
VERSION     DACDTH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.044"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            156..1862
                     /locus_tag="IWK54_001799"
     CDS             156..1862
                     /locus_tag="IWK54_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9593919.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IWK54_001800"
     CDS             complement(2020..2922)
                     /locus_tag="IWK54_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9593920.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IWK54_001801"
     CDS             complement(2919..3623)
                     /locus_tag="IWK54_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9593921.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IWK54_001802"
     CDS             complement(3654..4295)
                     /locus_tag="IWK54_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9593922.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4602)
                     /locus_tag="IWK54_001803"
     CDS             complement(4333..4602)
                     /locus_tag="IWK54_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593923.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            complement(4575..5264)
                     /locus_tag="IWK54_001804"
     CDS             complement(4575..5264)
                     /locus_tag="IWK54_001804"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9593924.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IWK54_001805"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IWK54_001805"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9593925.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="IWK54_001806"
     CDS             complement(5984..6916)
                     /locus_tag="IWK54_001806"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9593926.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="IWK54_001807"
     CDS             complement(6913..7689)
                     /locus_tag="IWK54_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9593927.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IWK54_001808"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IWK54_001808"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9593928.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IWK54_001809"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IWK54_001809"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9593929.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="IWK54_001810"
     CDS             complement(12014..13294)
                     /locus_tag="IWK54_001810"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9593930.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="IWK54_001811"
     CDS             complement(13313..14239)
                     /locus_tag="IWK54_001811"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9593931.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="IWK54_001812"
     CDS             complement(14252..15535)
                     /locus_tag="IWK54_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9593932.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IWK54_001813"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IWK54_001813"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9593933.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="IWK54_001814"
     CDS             complement(16323..17234)
                     /locus_tag="IWK54_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9593934.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="IWK54_001815"
     CDS             complement(17231..17695)
                     /locus_tag="IWK54_001815"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9593935.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="IWK54_001816"
     CDS             complement(17700..18398)
                     /locus_tag="IWK54_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9593936.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="IWK54_001817"
     CDS             complement(18385..18771)
                     /locus_tag="IWK54_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593937.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="IWK54_001818"
     CDS             complement(18918..20588)
                     /locus_tag="IWK54_001818"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9593938.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="IWK54_001819"
     CDS             20771..21004
                     /locus_tag="IWK54_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9593939.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="IWK54_001820"
     CDS             complement(21103..21534)
                     /locus_tag="IWK54_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9593940.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="IWK54_001821"
     CDS             21613..22026
                     /locus_tag="IWK54_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9593941.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
BASE COUNT         6238 a         4926 c         3892 g         6988 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttcaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggag
//
LOCUS       DACDTH010000045        22031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDTH010000045 DACDTH010000000
VERSION     DACDTH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.045"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            367..555
                     /locus_tag="IWK54_001822"
     CDS             367..555
                     /locus_tag="IWK54_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593942.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="IWK54_001823"
     CDS             545..805
                     /locus_tag="IWK54_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593943.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="IWK54_001824"
     CDS             846..1073
                     /locus_tag="IWK54_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593944.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="IWK54_001825"
     CDS             1138..1710
                     /locus_tag="IWK54_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593945.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="IWK54_001826"
     CDS             1711..2022
                     /locus_tag="IWK54_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9593946.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="IWK54_001827"
     CDS             2013..2273
                     /locus_tag="IWK54_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593947.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="IWK54_001828"
     CDS             2434..2793
                     /locus_tag="IWK54_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593948.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="IWK54_001829"
     CDS             2790..4433
                     /locus_tag="IWK54_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9593949.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="IWK54_001830"
     CDS             4468..5550
                     /locus_tag="IWK54_001830"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593950.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="IWK54_001831"
     CDS             5594..6766
                     /locus_tag="IWK54_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9593951.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="IWK54_001832"
     CDS             6753..7457
                     /locus_tag="IWK54_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9593952.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="IWK54_001833"
     CDS             7462..8622
                     /locus_tag="IWK54_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9593953.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="IWK54_001834"
     CDS             8698..8976
                     /locus_tag="IWK54_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9593954.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="IWK54_001835"
     CDS             8957..9328
                     /locus_tag="IWK54_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP9593955.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="IWK54_001836"
     CDS             9344..9748
                     /locus_tag="IWK54_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593956.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="IWK54_001837"
     CDS             9745..10155
                     /locus_tag="IWK54_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593957.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="IWK54_001838"
     CDS             10216..10803
                     /locus_tag="IWK54_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9593958.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="IWK54_001839"
     CDS             10876..11343
                     /locus_tag="IWK54_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAP9593959.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="IWK54_001840"
     CDS             11389..11697
                     /locus_tag="IWK54_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593960.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="IWK54_001841"
     CDS             11892..18626
                     /locus_tag="IWK54_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9593961.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="IWK54_001842"
     CDS             18630..19502
                     /locus_tag="IWK54_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593962.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20126
                     /locus_tag="IWK54_001843"
                     /pseudo
     CDS             19499..20126
                     /locus_tag="IWK54_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20087..20548
                     /locus_tag="IWK54_001844"
     CDS             20087..20548
                     /locus_tag="IWK54_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593963.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="IWK54_001845"
     CDS             20552..21376
                     /locus_tag="IWK54_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593964.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..21939
                     /locus_tag="IWK54_001846"
                     /pseudo
     CDS             21394..21939
                     /locus_tag="IWK54_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DACDTH010000046        21988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDTH010000046 DACDTH010000000
VERSION     DACDTH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.046"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IWK54_001847"
     CDS             <1..395
                     /locus_tag="IWK54_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593965.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="IWK54_001848"
     CDS             398..1147
                     /locus_tag="IWK54_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9593966.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="IWK54_001849"
     CDS             1159..1428
                     /locus_tag="IWK54_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593967.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1590..1892
                     /locus_tag="IWK54_001850"
     CDS             1590..1892
                     /locus_tag="IWK54_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593968.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="IWK54_001851"
     CDS             1889..2050
                     /locus_tag="IWK54_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9593969.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="IWK54_001852"
     CDS             2047..2352
                     /locus_tag="IWK54_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593970.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="IWK54_001853"
     CDS             2352..2708
                     /locus_tag="IWK54_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP9593971.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="IWK54_001854"
     CDS             2668..2913
                     /locus_tag="IWK54_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593972.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="IWK54_001855"
     CDS             2910..3329
                     /locus_tag="IWK54_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9593973.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="IWK54_001856"
     CDS             3326..3883
                     /locus_tag="IWK54_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9593974.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="IWK54_001857"
     CDS             3880..4176
                     /locus_tag="IWK54_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593975.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="IWK54_001858"
     CDS             4253..4666
                     /locus_tag="IWK54_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9593976.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="IWK54_001859"
     CDS             5012..5386
                     /locus_tag="IWK54_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593977.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5616..5960
                     /locus_tag="IWK54_001860"
     CDS             5616..5960
                     /locus_tag="IWK54_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9593978.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="IWK54_001861"
     CDS             5965..6246
                     /locus_tag="IWK54_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593979.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="IWK54_001862"
     CDS             6349..6663
                     /locus_tag="IWK54_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP9593980.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="IWK54_001863"
     CDS             6641..8335
                     /locus_tag="IWK54_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9593981.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="IWK54_001864"
     CDS             8355..9533
                     /locus_tag="IWK54_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9593982.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="IWK54_001865"
     CDS             9496..10182
                     /locus_tag="IWK54_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9593983.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="IWK54_001866"
     CDS             10182..11342
                     /locus_tag="IWK54_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9593984.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="IWK54_001867"
     CDS             11352..12227
                     /locus_tag="IWK54_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593985.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="IWK54_001868"
     CDS             12224..12535
                     /locus_tag="IWK54_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593986.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="IWK54_001869"
     CDS             12525..12878
                     /locus_tag="IWK54_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9593987.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="IWK54_001870"
     CDS             12868..13269
                     /locus_tag="IWK54_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593988.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="IWK54_001871"
     CDS             13262..13666
                     /locus_tag="IWK54_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593989.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="IWK54_001872"
     CDS             13678..14286
                     /locus_tag="IWK54_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9593990.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="IWK54_001873"
     CDS             14306..14668
                     /locus_tag="IWK54_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593991.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="IWK54_001874"
     CDS             14671..14853
                     /locus_tag="IWK54_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593992.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="IWK54_001875"
     CDS             14870..18289
                     /locus_tag="IWK54_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9593993.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="IWK54_001876"
     CDS             18340..19077
                     /locus_tag="IWK54_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9593994.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="IWK54_001877"
     CDS             19087..21378
                     /locus_tag="IWK54_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593995.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="IWK54_001878"
     CDS             21402..>21988
                     /locus_tag="IWK54_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9593996.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3246 c         4705 g         6104 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatctg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DACDTH010000047        21667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDTH010000047 DACDTH010000000
VERSION     DACDTH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.047"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(375..2756)
                     /locus_tag="IWK54_001879"
     CDS             complement(375..2756)
                     /locus_tag="IWK54_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9593997.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2746..3393)
                     /gene="recU"
                     /locus_tag="IWK54_001880"
     CDS             complement(2746..3393)
                     /gene="recU"
                     /locus_tag="IWK54_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9593998.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3457..3996
                     /locus_tag="IWK54_001881"
     CDS             3457..3996
                     /locus_tag="IWK54_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9593999.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4086..4505
                     /gene="gpsB"
                     /locus_tag="IWK54_001882"
     CDS             4086..4505
                     /gene="gpsB"
                     /locus_tag="IWK54_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9594000.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4527..4894
                     /gene="rnpB"
                     /locus_tag="IWK54_001883"
     ncRNA           4527..4894
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWK54_001883"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5103..6269
                     /locus_tag="IWK54_001884"
     CDS             5103..6269
                     /locus_tag="IWK54_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9594001.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6315..7811
                     /locus_tag="IWK54_001885"
     CDS             6315..7811
                     /locus_tag="IWK54_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9594002.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7892..8575
                     /locus_tag="IWK54_001886"
     CDS             7892..8575
                     /locus_tag="IWK54_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9594003.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9220..10410
                     /locus_tag="IWK54_001887"
     CDS             9220..10410
                     /locus_tag="IWK54_001887"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9594004.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10512..11465
                     /locus_tag="IWK54_001888"
     CDS             10512..11465
                     /locus_tag="IWK54_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9594005.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11534..12883
                     /locus_tag="IWK54_001889"
     CDS             11534..12883
                     /locus_tag="IWK54_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9594006.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13066..13389)
                     /locus_tag="IWK54_001890"
     CDS             complement(13066..13389)
                     /locus_tag="IWK54_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594007.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13518..14681
                     /locus_tag="IWK54_001891"
     CDS             13518..14681
                     /locus_tag="IWK54_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594008.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14751..16100
                     /locus_tag="IWK54_001892"
     CDS             14751..16100
                     /locus_tag="IWK54_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9594009.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16145..16576)
                     /locus_tag="IWK54_001893"
     CDS             complement(16145..16576)
                     /locus_tag="IWK54_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9594010.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16964..19606
                     /gene="alaS"
                     /locus_tag="IWK54_001894"
     CDS             16964..19606
                     /gene="alaS"
                     /locus_tag="IWK54_001894"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9594011.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19806..20402
                     /locus_tag="IWK54_001895"
     CDS             19806..20402
                     /locus_tag="IWK54_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9594012.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20524..>21667
                     /locus_tag="IWK54_001896"
     CDS             20524..>21667
                     /locus_tag="IWK54_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9594013.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLL"
BASE COUNT         7017 a         3812 c         4565 g         6273 t
ORIGIN      
        1 cagacaaatt atgataatta agcatactat cagataactc aaaatgaagc cttctttcaa
       61 aaaattattc aaaaccatat attgtatttt ttatttgata ataacaatgg gattattaag
      121 aagtggcact aaataaaaga ctagtgtgta tttagccgat ttaattataa acaagtaaat
      181 ggataaagta tatgtaatga taaaagaaaa atggattttc taatttttaa gctaaatgtt
      241 agatcacaca tgtatatgct ggatagtctt ataaaaaatc atgtaataca gcttggtaca
      301 tgatttttat ttgtagctgt tttttacata gacacaaaaa agaaaccacg cttgtagacg
      361 tggtttcttt tttactatct gtttccagaa gctgaggtgc tgctggatgg tgtatggtca
      421 tttgcagcat ccgctccgct agttgctggc tcactggaag aagcaggagg agcagatgaa
      481 gacggtgtcg agctttcttc ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg
      541 cttgtttctg ctgtcgtgct ttcttttgag gactcagcag ttgattcgct ttgactagta
      601 gagctagagc tttgagtcgt tgtctctgtt gttgagcttc caggagtctg gacatagctg
      661 gaagatggaa tagttgttga tggtgtaatt gcattcgatc tagctgtata ttgatctttg
      721 tagtacagtt caccgcccac acgaattaat ccgcttggca tctcccagtc tgtatttgtc
      781 acgttagctg aaacatactg cataagctcg cgataaacat cagaggctac gtgtgacgat
      841 tctgaagtga caggtgtcat ttttttattg taacctgtcc acacggaaat cgaataattt
      901 ggcgtatagc ccgcaaatag gatatctgga tacaccccag aagaaatacc aagttttgca
      961 tactcgtcat ctgtgtaatt ggatgttccg gtcttaccgg cttgatataa cccagcgatt
     1021 tgcgcattcg taccagttcc ttcagtgatc gtatctttta aaatgtctgt gatcatataa
     1081 gctgtttctg gagacatggc tgtttttcca tctggctcat aagtttcttc tgttccgtct
     1141 tggaagacga ttttattaac atattgaggt ttgtaataag tccctccatt ggcgaaagcc
     1201 gcataggctg ctgccatttt taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc
     1261 gtattactag agatcgcatt cgattgatgg atcgtactgt attcgatccc aagatttttt
     1321 aagaaagaag ccactttatc tgaaccgact tcattgaata atttgactgc tggaacatta
     1381 cgggataagt aaagtgcttg gcgtaaagta atggtgccca tatattgatt atcccagttt
     1441 cctacaggag tcgaagtccc ttcatagtta tacggtgcgt cagtaatagt ttttccagta
     1501 gaatatttca aatattcaaa agctggtccg taatctgtga caggtttcat cgtagaacca
     1561 aaatcacggg aagtattcac tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga
     1621 cgtccgccaa tctgggcttt tactttcccg gtattggtgt cgataagggt ggaggcaact
     1681 tgcatctcgt cgtctgggta agagacatac tgatccgtgt tgacgatatc atatagtttc
     1741 ttttgggcgt ccagatccaa attcgtataa atctctaaac cgtctgtgta gacattctta
     1801 tctgtttttt cttgtacttc attgatgact tctttgacat aattatcgac gatcttagta
     1861 ttgtcatcgg attgagttag ttcttgcaga ccatccgtta ccggtacatt aacagcttga
     1921 tcatattctg tttgagagat tttttcgttt tgaagcatcg tatacaaaac agtatctcgt
     1981 cttttcttcg cctgatctgg ataaacataa ggatcatagg cggaaggggc ttgaggcatc
     2041 ccagcaagca atgctgtttg cggtaaagaa agttctgata atttcttacc aaagtacatc
     2101 tcagaagctg tctccatccc gtacaagccg tttgacatat acactttatt tacgtagtag
     2161 gtcaagatct cttgtttcga ttttttttgt tccaatctta cagccatcca cgcttcttgg
     2221 gccttgcgtt tcagagtctg atcctcggca ctggtggaga agaaggaaag tttgatcaat
     2281 tgctgtgtca aagtacttcc gccttgcagt ccgccagagg taaagttaga gagggcagaa
     2341 ccgatgatcc gaatagggtc aaccccgatg tgtttataaa aacggcgatc ttctaccgac
     2401 acgatcgcat cttctaatgt tttcggtaat tcattcgcag aaattttttc acgcttttct
     2461 gcgcctaaat cttcgaatag ttcgccatct tgtgtatata gtttggaaga tacggtagcg
     2521 tctaattttt tatccgtcaa ttcgggcgca tcttttgcgt aataccagaa aagtcctaca
     2581 cctgctaaaa agaggataca gaaaaatgag agtattccta aaaggatctt gataaataat
     2641 ccttttttat gtgttccaga ggactttcca gaacctttgc cagaattctt cttcacactc
     2701 tttttaggag taggctgttt ctgtcgtcta gagcttctag tttgttcatt tgccattctt
     2761 gctttctcct tcaatagata atgttgataa atagaagtca accgcgtcca aataaggaat
     2821 ccgcggagaa ataccagttt tgatctctat tcctagttct tcgatcaaag ataaaggtag
     2881 tgatttgcgt cctttttcct tttgggcgtc ccagtaagca atcaaatgaa cactattcaa
     2941 ataaaaacag cgcttcaaag acgaaaacca taacaagaca aagcagaccc cattttgttc
     3001 gaggcatgct ttcatatgtt cgatttgatg ggcatggaag tttttaaagg gaaaagatgt
     3061 tttgttcttg gtttcttttg cttcaaagtc caagtaaaag cctcgataga cgccattata
     3121 gtctgtagtt gaggcttggg taaagtaggc ttccttgatc acagcagcac ttctcttagg
     3181 atagtctact ttcacgattt ggataggggt aggcttttta tggataaccg ccaacccgcg
     3241 ggaaagataa tatttattac tttcattgat tgcttcttca aagcgcatcc cccgattgcc
     3301 gaattcagtg atctgatttc ttttttcttg aattttttcg acgggagagg cattcttata
     3361 ataaggttgg ccatttggat agcgcaatac catctttaat cacactccta ggccacatta
     3421 tagcaaaaaa gcggatagaa agaaaaggaa tctgagatgg ataagtcaaa agtaatctat
     3481 gtgtctgggt atcgaagttt cgaactcggt gtttttaaag aaaatgatcc taaaatccaa
     3541 gttgttaaaa atgttttaaa aaaagaactc catcagttag cagaagaagg gctggaatgg
     3601 gtacttgtca gtgggaattt gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg
     3661 aaagaagaat atccggaact atctcttggt attttgtatc ctttttctga ttttggtaat
     3721 aactggaatg aagggaacca agaaaaaaaa caattagcag aacaattagc agattatgtc
     3781 gaagctgtta gccatcaacc ctaccaatca cctagtcagc taaagaatca cactcgtttt
     3841 atacttgagc atacagtggg ctgtctgctg atctatgatg aagaattccc tggaaaaacg
     3901 aagtattttt ttgaagatgc acagaattac caagcagatc atccctacga aattcggctg
     3961 atcagtatgg acgatcttca aaattttagt gaataatcga tattgtgttt gatttatcaa
     4021 cttttttcct atacaatgaa aaaggtagaa atgttattac gtaaatgaaa actagaggtg
     4081 taaatatggc gaatttggtt tatagtccta aagacatttt acaacaagaa ttcaaaacaa
     4141 aaatgcgcgg ctatgatccc gttgaagttg atgaattctt agacaatatc atcaaagatt
     4201 atgaagctta cagcaaagaa ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa
     4261 aagtagctca attatctaaa actcaaggag cagctcaaac acgcgtgcaa caaacagagg
     4321 caccaaagag cgcagcagtg accaattttg atatcttaaa acgcttgtca aatcttgaac
     4381 gtgaagtatt tggtaaaaag cttgatcaac aagcaagtgc agtcaaacca gcacaaccta
     4441 atccaaataa ctacacaaat gcagacacaa gcttagatga taatgaaaaa acacgtcaat
     4501 tttaattttt gattaatgaa tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc
     4561 tgaggaaagt ccatgctcgc acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc
     4621 ataagctaag gtactctttg gagtaacggc aggaaaacaa gctaaggctt cggctatgct
     4681 taagtatcct tgaaagtgcc acagtgacga agcggctggg gaaacccatc cggtggaacg
     4741 cggtaaaccc ctcgagcgag caacccaaac aatggtaggg gcgctttttg tctggaagaa
     4801 gaacaggaca aaaaggcagt ataactgcag atagatggtt accgctttgt tttttctccc
     4861 tggaaaagca aagtacagaa catggcttat agaaaaccgc aaacatcagg gaattaggtc
     4921 gtgacaaaag taatgcgtta taagaaacaa gaaaaacatt tgaaagctat ttttcaatac
     4981 ttcagatgaa tcgttcttat tacaaaacaa acttttgtat caccggttat tcggaatttg
     5041 agtaaaagtg tggggtagga ctgatgtccc acctcctttt tttataaata aggagattgt
     5101 gtatggaaaa aaacaggaag ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac
     5161 tggttggtaa agaattaaga gatcttggaa tcgaatgcca agttgagaac ggacgtgcac
     5221 gttttgaagg aacaatggaa acgatcgcta cagctaattt atggttacgt acagcagatc
     5281 gagtaaaaat cgtggttggg gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg
     5341 taaaagcatt gccttgggaa gattttcttc cactagatgc agcttttcct gtagctggaa
     5401 aatcgatcaa gtcgaaacta tatagcacac ctgattgcca agcgatcaca aaaaaagcaa
     5461 tcgttgaacg tctccgtacc tattatcacc gtccagcttc tgtaccactg acggaaactg
     5521 gcgcacattt tcagttggaa gtagctttgc tgaaagacca tgtgatggta actctggata
     5581 cgacaggacc aagtttattc aaacgtggtt atcgcttaga aaaaggtgga gcaccattga
     5641 aagaaaacat ggcagcagca cttattatgt tgacaaattg gcgaaaagac cgtccatttt
     5701 atgatcctgt atgtggttca ggtacgcttt gtattgaagc agccttaata ggccataata
     5761 tcgcaccagg attcaatcgt gagtttgctt gcgaatcttg ggattggttc agtcaagaaa
     5821 tcatggaaaa tgttcgatca gcagccgaag aaaaagcaga ttatgatatt gaattagata
     5881 ttactggttc agatatcaat ggacggatga ttgaaattgc caaagccaat gcagaagaaa
     5941 tcggattagg ctcttcgatt acctttaagc aacaagcagt caaagacttc aaaacggata
     6001 aagaatacgg ggtcatcgtg gctaatccgc cttacggaga acgtctagga gaagaagaaa
     6061 ctgttcggaa attatataaa gaaatgggcg atgtattccg tccattaaaa acatggagta
     6121 aatatatctt gactagcgac ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa
     6181 aaagaaaatt gtacaatggt gcattgcgta cggatctatt ccagtattgg ggaactcgtc
     6241 cgcctagaaa aaaagagaaa gacgaataat cttactaatt gattcattca attggatgtt
     6301 tggaggaata gggaatgaaa gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc
     6361 tacaacaagc tttaggaatc ctcgattggg atacacaaac aggtatgcct gaaaaagcta
     6421 gtgcataccg aagtgaagta gacagctatc tttacagcat ttatttttca aaaaaaatcg
     6481 gtccaaaaat caaagaagcc attcgctatt tttctgaaca tccggaagaa ttgtcggaag
     6541 ttggaactgc cgtttttgcc aaagtaaaag aagagtatga tttggaacat cgcgtaccgg
     6601 aagatcttat gagaaaatat acagctgccg tttcttcagc acacggacaa tggcaaaaag
     6661 ctagagaaac acaacaattt tctgattttc aacacgcatt gaatcaaaat attgaattaa
     6721 caaaacagtt gataccttac tggagaaaag atgaaaagac gaattatgat gttttgctga
     6781 atcaatacga acccgggatg actgtcgaaa tccttgataa ggtatttgat cagcttaaag
     6841 aaggaattct ttcgatccgc cgtacgctcc aagaaaaagg gactgaacct gaaacagact
     6901 ttttaaatcg gagaatgacg aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac
     6961 aacttggtta tgattttagt cgtggtcgtt tagatgacac tgtccaccca tttatgactg
     7021 gaattaatcc gaatgatgca cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg
     7081 ctgtttttgg agtgatccac gaggctggtc atggtatgta tgaacaagac atcgatgata
     7141 aatatgcgta tacaccggtt tatgaaggta catcaatggg tatccatgaa tcacaatcgt
     7201 tgtttaacga gatcattatt ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct
     7261 ttcaagaatg tgcagaagga acattcgatg atattccttt tgacaaattt tatcgttcat
     7321 tgaaccatac aaaatcaagt ttggttcgta tcgaggcaga cagtttgact taccctctcc
     7381 atatcatcat tcgttatgaa atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg
     7441 aactacctga aatatggaat caaaaatatg aagagtatct gggaatacga ccaacaaatg
     7501 atgtggaagg catcctacaa gacgttcatt ggtcaggtgc aagctttggt tattttccct
     7561 cttatgcatt agggtacatg tatgcggcac aattacttca ttctttgcag caagaaatag
     7621 atgtggaaaa tgttctagcg tcagacgatt attctccaat caaaaactgg atgtcagagc
     7681 atatccatca atatggtgca tctagaaaac caaatcaatt aatcatggat gcaacacatg
     7741 aaccgcttaa tccgcaatac ctgatcgact atatgaaaga cctgtatttt aatgtatatc
     7801 aggtaaaata aaatacaatt tacaaagata gctgaaattc atctaatcta tagatgtttt
     7861 cagctattta cttaagaaag aagtggaacg aatggataaa ttacaagtga tcgctgatta
     7921 tagtatggac caattagcgc atgatcaaac aggacatggg agcgatcata ccaaaagagt
     7981 tgtcaagtta gctgaaaaaa ttttggagac ggaaccgcag gcagaccgct ttgtgacgct
     8041 tgcagcagct tacctacatg atacgattga tgataaagtc gttaaaaatg agaacgaggc
     8101 aaagcaacag cttcgtgtat tccttcgctc tttgccaata gtagaggaac agatctcgat
     8161 gattttttcc attatcgaaa atatgtcctt ttctaaaaat ctctctgaag cggccgaact
     8221 atctctggaa ggtaagatcg ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg
     8281 cattttgcgt actgcttatt tcggcggtgg acatgggcac ccgatttttg atcctgaact
     8341 gtatccgcaa acattcaagg acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca
     8401 tttttatgaa aaactgttct tgctgtctga taaaatgaat actgattacg gatatgaaga
     8461 agccaaacga agagaaacct tcatgcgtga attcttagaa gaattttttc tggaatggtc
     8521 tattgacgat gaaaatttta ctccagacaa ttggatcaaa aataacaaaa aatgatggag
     8581 gttgtgacag aagtggtcag ctccaagata tttcggatca ttttccgtaa aagttacttc
     8641 tggaacactg tttaccaaga ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca
     8701 atttcaaaaa atagcttctc gtgtttgatg aatattgtaa cttgtgattc ggaattggca
     8761 ggagaacacc gtttattcgg aagaatagga ctgtgacaag acttttgtca cagtcctttt
     8821 ttcgaattca acaagtaaaa cttttaagaa atattcagag agtaaaatta aaaattgctg
     8881 ataaacgaag atggaaattt tatgtatttt tattggtcta tgcaataaat aaattaaata
     8941 ttcagaatac aattgatttt aacgaaaggg cttctatcaa tggttgtgtt gtatttatct
     9001 gcccaggtag agtaaaatag atcattatct aaaaggtaat tgttcatact atataagtca
     9061 tttgaaagta aaaagatact gtaaataaaa aatgtggtta aataattctc ataatcagtg
     9121 taagcgttat caaaaatata gcatacttgc tttgtagatg aatgacacga tatatgtttg
     9181 gtatgttctg caagacagat gaatatggag gtggagacaa tgtcttccaa agaactaaca
     9241 atgtatttag agaaagaatt ggatcaactg aaagaaaagg ggttatacaa tacgatcgac
     9301 atactagaaa gtgaaaatgg tgcttgtatc atcgtagacg gaaaaaagat gatcaatcta
     9361 gcatccaaca attatcttgg atttgccaat cgcgaagaat tgaaaaaagc atgtattgaa
     9421 gcaaccgaaa cttatggtgt aggagcagga gcagtccgaa cgatcaatgg ttcgctgaaa
     9481 atccatcagc aattagaaga aaaaatcgca gaattcaaag ggacagaagc agcaatcgcc
     9541 tttcaatctg gattcaactg caatatgggc gctatctctg cagtaatgac aaaagaagat
     9601 gctatcttat cggatgaatt aaaccatgca tctattattg acggttgccg cttatccgga
     9661 gcaaaaatca tccgaattaa acatcaagat atgaaagacc ttgagaaaaa agcaaaggaa
     9721 gcgatagaaa gtaagaaata taaaaaaatc atgtatatca cagatggtgt tttctccatg
     9781 gatggagaca ttgcacgatt gccggaaatt attccgattg ttgaaaaata tggactgatc
     9841 acctacgtcg atgatgcgca cggctcaggc gtaactggaa aaggtgcagg aaccgtcaag
     9901 cattttggat taagcgacaa aatcgatatg caaatgggca cgttatcgaa agctataggt
     9961 gttgttgggg gttatgtagc tggatcgaaa acactaatcg actggttgaa agctagaagt
    10021 cggccattcc tattttctac ttcgctaact ccaggtgcag ctgcagctgc attggcttcg
    10081 attactttga tgcaagaaca tccggaactt gtcgaaaaag tttgggaaaa tgctaattat
    10141 tttaaagaag aattgaaaaa agtaggctat aacatcggta tgtctgaaac acctattact
    10201 ccagtcattt tgggagacga aaaagtgact cagactttct ctaagaagct gatcgaacat
    10261 gggatttatg ctaaaccaat cgtttatcca actgtgcctc ttgggacagg caggatcaga
    10321 aatatgccga ctgcggaaca tacgagggaa atgcttgatg aagcagtagc agtttatcaa
    10381 aaaataggaa aagaaatgga aattatttaa ctaaatttag ttttttcgtc tatttaaaat
    10441 tagagaacat gttcacgcat atattgttaa tcatctgaag atagtagtaa aaaagatcag
    10501 ggaggatcat catgaaaaaa gtattggtga caggatgttt aggacaaatt ggttcagagt
    10561 tgatcacacg gctacgtcaa gaactaggca gtgaaaatgt cattgcgaca gatatacgca
    10621 tgcctgaagt cattgagaaa aacgatatct ttgagatcct agatgtaatg gattacgaaa
    10681 aaatgcgcag catagcagaa caatatcaag tggacacatt gattcatttg gctgctcttt
    10741 tgtcagctgt tgcagaagca aaaccacaat tagcctggaa tttgaacgtg actgggctgg
    10801 tcaatgcatt ggaagtcgct agagaattcc agctgaaatt ttttacgcca agttcaatcg
    10861 gcgcgtttgg tccaaatact ccaaaagatc ataccccaca agatactctt cagcgtccta
    10921 caaccatgta tggggtaacg aaggtagctg gagaactact atgtgattat tattatgtga
    10981 aatatggcgt agatactcgt ggtgtccgtt tccctggatt gatttcttat gcaaccttgc
    11041 caggaggcgg tacgacagac tacgcagtcg atatttatta ttcagcaatc aaagaaggaa
    11101 agtacactag tttcattgat caaggtacat caatggatat gatgtatatg ccagatgcga
    11161 tcgatgccat tataaaactc atgaatgcag atcctgatcg attggttcat cgaaatgcct
    11221 tcaatatatc ggcgatgtct tttgacccgg aagagatcaa agcatctatt caaaaaatca
    11281 taccagattt tgaaatggat tatgatgtgg atcctgtacg tcagtcaata gcagaatctt
    11341 ggccaaattc actagatgtt tcttgtgcgc aagaggaatg gggcttttcg ccgaaatacg
    11401 acttagatgt gatgacaaag gatatgttgg aaaaactgaa agaaaaattg gtagagacgg
    11461 tataaaacaa gaaccacatc caaaaagttt tttatacttt tgggattggc tatggtatga
    11521 aaggggacaa gggatggaag aatatcaagc cacgccagta aaagaagtaa aaatagaaaa
    11581 tgaattaaaa cgtacgatgg gattttttac tgctttatcg actgttatgg gcacagtgat
    11641 cggtgcaggg gtatttttta aagccgcaag cgtagcagaa gttacaggat ccgccagttt
    11701 gcatatgttt tcttggtttc tcggtggtat gatttctgtc tgtgcaggac taacaggagc
    11761 tgaactggca gctgctattc cagaaacagg agggatgatt aaatatatcg aacggattta
    11821 cggaaataca gcagcttttt tattgggctg ggcgcaagtc gtgatttatt ttccagcaaa
    11881 tgtagcagcc ttgtcgatca ttttcggtac acagttcgtc aatctatttg gtctttccca
    11941 gtcaatgatt gttcccgtag ctgttacagc tgctgtatcg atcctgttga tcaatttttt
    12001 aggttctaaa gctggagggg cttttcagtc gattacgctt gtctgcaagc ttattccttt
    12061 gtttgtgatc gtcattttcg gtttgttccg acaagaagga gtagacttcc aactgtttcc
    12121 gatccaagca ggagaaaatc tttcattttt ctcagccttg ggagcgggct tgctagcaac
    12181 gatgttcgca tacgacggtt ggatacacgt aggaaatatt tcgggagaat tgaaaaagcc
    12241 ggcgaaagat ttgccaaaag caatttcttt aggaatcatc ggaatcatga tcgtttattt
    12301 attagtaaat gcggtgttct taagaaccgc atctatcgac ggagtcgcag gaaacagcaa
    12361 tgcggcaagt gacgttgcaa aaatgatttt tggcggcttc ggtggaagac tggtcacagt
    12421 tggtatcttg atttctgttt atggaaccat caatggctat acccttactg gaatgcgtct
    12481 cccttatgtt atggcaaaag aaaacaacct gccttttagc aagctctttg ctaaacttca
    12541 tgataaaact aaagttcctg tagccgcagg tatcttagag ctagtcatcg ctattggcat
    12601 gatgatgatc ggtggttttg atacactaac ggatatgctg atctttgtca tttggatttt
    12661 ttatacgatg gtctttgttg gtgtcattct tcttcgtaaa aaagaacctg atttgtttcg
    12721 accatacaaa gttccaatgt atccgtttat tccactagta gcaatcattg gagggacttt
    12781 tatcgtatca agcacgctta tcactcagac ctttttagct tcgatgggga tagtattgac
    12841 acttgctggt atcccgattt atctctattt aaaacgtaga tagctaaaac attcgaattt
    12901 tattagaact gcacttctct gccaaacaaa atattttatt taaatgagga gtacgtggaa
    12961 gaaatgtcaa aagtactcgt taattttgat cacgtaaatg tcgttttgtc tacgatgaaa
    13021 tagcaaagta aaagacagaa atcgggctca accgatttct gtcttttact ttgatttgcg
    13081 taactgaact attcggcgta cgccgcgata aatatttaat aaaaccaagc aaccagaaat
    13141 gaaaatggga agccaaatca gatagacagg gtagccaagc actttagaca aagcatgtgg
    13201 agtcatttta tccatttcaa gaagtgcttg ctgccaagtc tgaagattga agactagcat
    13261 gaaacccgaa ataatcagga gaataaatcc taaataatga attgtagttc tttttcttac
    13321 ataagcgatc gtagcataaa ggaacagtaa aaaaataggt acgccaaaaa caaagtacat
    13381 attcagcatc tcgacacccc ctttgtgttt atcttacttt actttgaaag aaatgagaaa
    13441 taatagcctc gttacttgct tgaaacagca aaggactgct aagataagag agatacaaag
    13501 aatcaaggag gagtcatatg gcaagttata tcgaaccgat tcgttttgcg ctgattgttt
    13561 ttccattttt agccttagcg atctcatctg ttttttttat ttatgaatat cgaaagtatg
    13621 gaacatttct agtaacaagg gcagttattt tgtattcttt cgttttttat ttattgtgtg
    13681 cgtatttttt ggtgatctta ccacttccgg caaaggagac ggttgcccaa atgacagggc
    13741 caaagatgga gctgcattta tttgcttcat ggcaacattt cagagacaac acggtattgg
    13801 tcttgtctga acctagtacc tacctgccgg caatgaagca gagtgtattt ttggaaccag
    13861 cttttaatgt tttattactc ataccatttg gcatttattt aagatattat ttccggcttt
    13921 cttttatgaa aacgatgatt gctagtttct gcctttctct attttttgaa ttgacgcagc
    13981 taagtggact ttattttatc tacccaagac cataccgctt atttgatgtg aatgatttag
    14041 tgacaaatac attgggtgga atcattggtt ttgtcgttac gccgttattt acatttatgc
    14101 ttcctacaag aagacgaatg gatgaacttt cttatgaaaa aggaaagact gtctcattga
    14161 ctcgacgctt ggtcgcttat ttgatcgact ggacattttt atctcttatt aatttggtag
    14221 cggcgatttt ctttaaaatt gcgttcgatt caggggcagt tacttctcat atctggtggc
    14281 ttttattaga agtaggggta tactttatga ttgtaccgtt tataaccaat ggatggacac
    14341 ttggcaaaaa aattgtgcgt atccgtttgg tggaagaaga ccgcgaacga atttctttaa
    14401 aagcattgat gatccgctac gggtatctgt actttctttt ttatggttta agctggttgg
    14461 ggcagttgct tcagcaaatc atgaattcga caacgaatac ggtgttgcag atgagtgcag
    14521 tattcatttc tctcggtgtc ttgttactac aaggtatttt tattttaaat atcttgctat
    14581 catttttgag aaaaaaacgg attctgtttt atgaaaaagc tagtcacaca tatactgtta
    14641 gcacgattgg ccaaacaaag gatgatgatt ctcgaaatta gctgagcaaa aacgctttgt
    14701 ctgtgttata atgccaaagt cgtgataatt taaagagaag aggtgcacga atgaataatt
    14761 ttgatatatt ccgcttcaaa ccgtttatca atgaagcgtt aaccgcaaag ggcttcaaac
    14821 agcccacaga ggtccaggaa cgtttgattc ctttaattgc aaaaggaaaa aatatcattg
    14881 ggcaatctca aactggtaca ggaaaaacac atacgttttt gttgcctttg tttgaaaaaa
    14941 tcaatgcaga aaaagaggaa gtacaagttg ttattacagc tccaagtagg gaactagctg
    15001 cacagatcta tgaagcagct acacaaatcg ctaaattttc tacaccggag atccgtgtct
    15061 cgaattttgt cggcggtaca gacaaacagc gtcaattaaa tcgtctgcat caccaacagc
    15121 cacaggttgt agtaggcacg cctggtagaa tattagacat gatgaatgaa caagcgctga
    15181 agattcacac cgcatttgca tttgttgtcg atgaagcgga tatgacattg gatatgggtt
    15241 ttttaaatga agtagaccaa attgcaagtc gattaccaga aaagctgcag tttttagttt
    15301 tttctgccac tattccagaa aaacttcgtc cattcttacg aaaatacatg gaaaatccaa
    15361 tcattgaaga aatcaaacca aaagcagtta tttctgaaga cattgaaaat tggctgattt
    15421 caacaaaagg taaagatgag aatcaaatga tttatcaact gctgacaatt ggtcatcctt
    15481 atttagcaat cgtatttgcc aatacaaaac agcatgtgga tgaaattgcc gattacttga
    15541 aaaaccaagg cttgaaagta gcaaagattc atggagacat caccccaaga gagagaaaac
    15601 gagtgatgcg acaagtacag aatctggagt atcaatatgt tgtagcgact gatttagctg
    15661 ctagagggat cgatatcgaa ggtgtctctc atgttattaa tgcagaaatt ccagaagatc
    15721 tagacttctt tatccatcgt gtcggacgta ctggacgtaa tggcttaaaa ggaacggcca
    15781 ttacgttata ttcgccaaat gatgagcaag ccattgatgc gatagaaaaa ttgggcgtct
    15841 catttgaacc aaaagaaatc aaaaacgggg aaatcgtcaa aacatacgat cgtaatagac
    15901 gtgccaaacg agaaaaatca aaagatgtat tagatcccac gctgattggt cttgtgaaga
    15961 agaaaaagaa aaagatcaag cctggttata agaaaaaaat cgattgggca attgcagaaa
    16021 aaaataaaaa agaacgcaaa atcgaacgca gacagcaaag ccgtactgct cgtaaaaata
    16081 agaaaaattc acataaataa aaaacaagag gttgtgacaa aaggcgtgtc acaatctctt
    16141 gtttttaagc tagacttttt ttcattccga tacaaagtac ttcatcttca ataaaaggtt
    16201 ctgtgcaagt atgataccct tgtttttcat aaaaatctac agccgtcatt tcagctacta
    16261 cataactagt ctgacaacct tctgaaaccg cccgtttttc agcagcttca agcaactgac
    16321 cgcctaaccc ttttcctcgg tattcttttg ctacgcaaaa tcgttccggc ttgaagcaag
    16381 taggtgtttt cttttcataa cgcaaagtag ctactggtct tttttgatca agaaatagat
    16441 aataggtttg ttgcgggata tcttttccgt cgaattctag ttctgcagga atcccttgtt
    16501 ctaatacaaa aacttgatat ctcaaaaagc agctagctgc gcgtatccat tcatccgctc
    16561 caactatttt tttcatcata ttcctccttg tcatgaatag cagagatgta ttgattcctt
    16621 tattcttatc gtaccaaaaa tcgtggagta tgaccaaaag gaaatgaaaa taaaaattga
    16681 gatattgaca gtgatcaata gaatgcgtat actaattaag tacgaaaagg atatgatttg
    16741 ttttttaaga ctcacagaaa attctttctt ggctgaaaaa agaatagtca ctaatcaaat
    16801 tgctcccttt ttctattttg gtagaaatac cgacgcagct cagcgttatc gagtttaaga
    16861 ggtaatgatt acacatcatt gcaatcaagg tggtaccgcg agttttcgtc cttggctgtg
    16921 atggtgtttt tttgtattta atttatagaa agcaggtaat gacatgaaac aattaagtag
    16981 tgcacaagtt cgacaaatgt ttcttgattt cttccaatct aaaggacata cgattgaacc
    17041 tagtgcgtct ttgatccccg tgaacgatcc aaccttattg tggatcaact ctggtgttgc
    17101 aacattgaaa aaatattttg atggttcagt tgtgccagaa aatccaagaa tcacaaatgc
    17161 ccaaaaatct attcgaacaa atgatatcga aaatgtcggt aaaacagcac ggcaccatac
    17221 gatgtttgag atgctgggta atttttcgat tggtgactat ttcaaaaatg aagccatcca
    17281 ctgggcatgg gaatttttga ctgatccaaa atggatggca ttcgatccag aaaaacttta
    17341 tgtaacagtt tatccaaaag atacagaagc aaaacgcatc tggcatgaag aagttggcct
    17401 gccactagat cacattattg atgtagaaga caacttctgg gatatcggtg caggcccatg
    17461 tggtccggat acggaaattt tctatgaccg tggagaagct tataacgatt tagcagaaga
    17521 cgatcctgaa aactatccag gaggagaaaa tgaacgttat ttagaaatct ggaacttagt
    17581 attttcagaa ttcaaccata agcctgataa cacatttgaa ccacttcctc ataaaaacat
    17641 tgatacgggg atgggattgg aacgcatggt ctcaatcgtt caagatgcgt caacgaactt
    17701 tgagacagac ttgtttatgc ctattatcca cgccgtagaa aagctaagca aacaagtaaa
    17761 atatggtgaa aacccagtaa cagatatttc ttacaaagta atcgcagatc atattcgcgc
    17821 cctttcattt gctattggcg acggtgcatt gccatcgaat gaagggagag ggtatgtctt
    17881 acgccgtttg cttcgtcgtg ctgtcatgca tgggaaaaaa ttaggtattg aacaagcatt
    17941 cttatatcgt ttagtccctg ttgttgggga aatcatggta agttattatc ctgaagtact
    18001 tcaacaaaaa gaatttattg aaaaagttat ccgtacagaa gaagaacgtt tcaatgaaac
    18061 aatcaatgaa ggtttggaaa tcttaaatca agtgatccaa gaagtaaaaa acgaaaacgg
    18121 gacgactcta gcaggaaaag acatcttcaa actatacgac acatatggct tcccggtaga
    18181 attaacggaa gaggtggcag aagatgaagg actgaaagtt gatcatgctg gttttgaaaa
    18241 agaaatggat gcacaacgcg aacgtgctcg ctctgcccgc agtactgaaa aatcaatggg
    18301 ggtacaatct gctttattga cagatatcaa agtagaaagt aaattcattg gatacacaga
    18361 acaagaatca gatagtcaat tattggtcat cgttaaaggt gagtctcttt tgaatgaagt
    18421 cgcagaaggt gaagctcaaa tgatctttga tcaaacacct ttctatgcag aaatgggtgg
    18481 gcaagtagcg gatcaaggat ggatcatgaa tgaagtaggc gaaatcgtag cagttgttac
    18541 agatgttcaa aaagcgccta atggtcaatt tatgcacacc gtagaagtaa aagcaccaat
    18601 gagtgaagga caaacctatc atctattgat cgatcaatcg atgcgcaatc ggatcatcaa
    18661 aaaccataca gcaacccatc tactgcacaa ggcattgaaa gaagtacttg gcgagcatgc
    18721 aaatcaagct ggttctttag tcgctcctgg acatttacga ttcgacttta cccactttgg
    18781 tcaaatcact tcagatgaat tagctgaaat ggaaagaatc gtcaacgaaa aaatctggga
    18841 agctatccca gttgaaacaa ttgaaacaga tatcgataca gccaaaaaca tgggtgcaat
    18901 ggctttgttt ggtgaaaaat acggacatga cgttcgagta gtaaatattg gcggctggtc
    18961 aatcgaactg tgcggaggca cacatgtagc aaatacagaa aacatcggaa tcttcaaaat
    19021 cgtttctgaa tcaggaattg gtgcaggcgt gcgacgaatc gaggctgtaa caagtaaaga
    19081 agcgtacgaa ctacttgaag aagaagaaaa acaactaaaa caagtagcaa caattgttaa
    19141 atcacctcaa ttgaaagaag ttgtttcaaa agcggaacaa ttgcaacagc aagtaagaga
    19201 gctacaaaag gaaaatgaac aattagctag caagttagcg aatcaacaag ctggtgatgt
    19261 attcaaagat ccagaaacga tcaatggaaa aacaatcatt gcagcacaag taaaagtgaa
    19321 agatatgaat cagctgcgtc aattagctga ccaatggaaa caaaaaaata tttcagatat
    19381 tcttgtttta gcaaacgaac aagaaggaaa agtcagtctg ctagcggcaa tgacgaaaga
    19441 atcaaatgaa gctggactaa aagctggcga cttgattaaa gcaatcgcac cgaaagtagg
    19501 cggtggcggt ggtggacgtc cagacatggc acaagctggc gggaaaaatc ctgctggtat
    19561 tccagatgca cttaaagctg cgaaagaatg ggttgcgcaa caatagggcg tttacgtttg
    19621 acagaaaaac aaaagttcaa acattgaacg aaacatgatt atgggggcga agacaaacaa
    19681 gtcttgccct catacttttt tcaattggat ggcgcatcta atcaaatggt cggattcgga
    19741 tggtctttag ttgacgaaag tgacagaaat ttttacgatt agtataaata aaaaggggtg
    19801 aaaaaatgaa tagtagaaat aagacctttc gactagtctt acgtgcgatc ttgttagcaa
    19861 tcatcatcgt acaagcgatg gttccttggc tggggtttat tccattagga ttcattagtt
    19921 taacaattat ccatatcaca gtaattattg ctgcagtagt attaggccca aaagatggca
    19981 tggtgattgg cttgttttgg ggaatcgcta caattgttcg tgcctatgcc atgccaacaa
    20041 cgccatttga tacattagtg ttcacgaatc cgattatctc ggtagttccc agagtacttg
    20101 ttggcttagt agcaggcttg gttttccact ggatttatca aaaaaataaa tcaataacga
    20161 tcagctcagc ctttgcaggg gtactgggtt cattggtcaa tacagtctta gtgttaggat
    20221 tcatgggatt attttataca ggtgcaacgg cagatgccta tggtgtagac tctgctcttt
    20281 tatttaaaac cttagcaggg gttgcagcaa tcaatgggat accagaagca ataggtgcgg
    20341 gaatcataac accattacta gcaaaagcgt tatttgcagc aacaccgttg aaaccagaat
    20401 aatgaccatc ttttaatagt aataggacgc acgatagata gaaaaaggaa gagaggagag
    20461 gaaaacaatc gtcctctcca tctttttacc tgagcaacaa gcaaactaga aggggtaaaa
    20521 aacttggcta agaaacaaga agaaaaaaag aaacaaatac cgcaacagcg gacaaaaaaa
    20581 ggagtactag cgggtatctt aggattggtt acgaatctga tcttgtttgt ctctaagcta
    20641 atgatcgggc ttttttcagg aagtgtctcc attatggctg atgcagtgaa cagtctatca
    20701 gatactgctt catccatatt gactttgatc ggatttaaaa ttgctgcaaa gccagcagat
    20761 caggaacatc cttatggaca cgaacgcttc gaatatatca gtgggttatt tgtttcgatc
    20821 atcattactt atgtaggatt tcaattttta gagtcttctg ctgaaaaaat catccatcca
    20881 gaaaatgtct ctttaacacc catcgtcttt tttgtcctaa tcatttccat ctttttgaaa
    20941 tttgcacaag gcaaaatgta tcaggttatt gcgcaatcca ttgattctga aacattgaga
    21001 gctacagcaa cagatagtta taatgatgtc tttactacac tggctgtatt gctttcagca
    21061 gcaatcgaat gggtaactgg atggcgtatt gatggatata taggttttgc tttagcatta
    21121 tatatccttt atagtggatt gacgatgctt cgaaattttg tatacgaact attaggaagt
    21181 cgcccaacgc cagcagagat tgaagaaatg gaagagcatt taaatgcttg cgatgatata
    21241 cttggctatc atgatttatt ggtgcataac tatggaccca ataaacgctt tgcatcagtc
    21301 cacatcgaag tagatgaatc aatgaatttg aatgtggctc ataagattat tgatctgata
    21361 gaaaaagact ttaaaaagtc tttggatgtg gaacttgtct gtcacttgga tccaatgcct
    21421 gttgataacg aaaaatacta taatatcttg cggcagttaa agcaaattct tcttgaaaca
    21481 ggtacagggc ttaatattca tgattttaga gtaattcatc aaggaaaaac gctgcaattt
    21541 gatgtagtgg tccctgagaa attccagttt gttgatacag aactagaaag aatcttacgt
    21601 agtcaaatag aagaaaaaat cggagattac caattggaaa ttacgtttga tcacaattat
    21661 cttttat
//
LOCUS       DACDTH010000048        20951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDTH010000048 DACDTH010000000
VERSION     DACDTH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.048"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..153
                     /locus_tag="IWK54_001897"
     CDS             <1..153
                     /locus_tag="IWK54_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9594014.1"
                     /translation="ENFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHI
                     YMKKTL"
     gene            complement(231..764)
                     /locus_tag="IWK54_001898"
     CDS             complement(231..764)
                     /locus_tag="IWK54_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9594015.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(858..2036)
                     /gene="mutY"
                     /locus_tag="IWK54_001899"
     CDS             complement(858..2036)
                     /gene="mutY"
                     /locus_tag="IWK54_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9594016.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2029..2826)
                     /gene="recX"
                     /locus_tag="IWK54_001900"
     CDS             complement(2029..2826)
                     /gene="recX"
                     /locus_tag="IWK54_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9594017.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2933..4315
                     /gene="rlmD"
                     /locus_tag="IWK54_001901"
     CDS             2933..4315
                     /gene="rlmD"
                     /locus_tag="IWK54_001901"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9594018.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4489..5067)
                     /locus_tag="IWK54_001902"
     CDS             complement(4489..5067)
                     /locus_tag="IWK54_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9594019.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5389..5859
                     /locus_tag="IWK54_001903"
     CDS             5389..5859
                     /locus_tag="IWK54_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9594020.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5967..7193)
                     /locus_tag="IWK54_001904"
     CDS             complement(5967..7193)
                     /locus_tag="IWK54_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9594021.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7947..8147)
                     /locus_tag="IWK54_001905"
     CDS             complement(7947..8147)
                     /locus_tag="IWK54_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9594022.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8281..8889)
                     /locus_tag="IWK54_001906"
     CDS             complement(8281..8889)
                     /locus_tag="IWK54_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9594023.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8974..9444)
                     /locus_tag="IWK54_001907"
     CDS             complement(8974..9444)
                     /locus_tag="IWK54_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9594024.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9512..10405)
                     /locus_tag="IWK54_001908"
     CDS             complement(9512..10405)
                     /locus_tag="IWK54_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9594025.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKNLFF"
     gene            10551..11354
                     /locus_tag="IWK54_001909"
     CDS             10551..11354
                     /locus_tag="IWK54_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9594026.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="IWK54_001910"
     CDS             11532..12479
                     /locus_tag="IWK54_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9594027.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="IWK54_001911"
     CDS             complement(12547..12840)
                     /locus_tag="IWK54_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9594028.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="IWK54_001912"
     CDS             complement(12868..13533)
                     /locus_tag="IWK54_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9594029.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="IWK54_001913"
     CDS             complement(13673..14305)
                     /locus_tag="IWK54_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9594030.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="IWK54_001914"
     CDS             complement(14312..15379)
                     /locus_tag="IWK54_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9594031.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="IWK54_001915"
     CDS             complement(15376..16107)
                     /locus_tag="IWK54_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9594032.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IWK54_001916"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IWK54_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9594033.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IWK54_001917"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IWK54_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9594034.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="IWK54_001918"
     CDS             18270..19439
                     /locus_tag="IWK54_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9594035.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..20620)
                     /locus_tag="IWK54_001919"
     CDS             complement(19706..20620)
                     /locus_tag="IWK54_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594036.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTF
                     KSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFS
                     KISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         6418 a         4130 c         3314 g         7089 t
ORIGIN      
        1 gaaaactttg ctaagaatca aggctataat acgattttat taggggcaca gtccacagct
       61 gaaacttttt acgaaaaact aggatataca gcttacggcg atccttttga agatgctggc
      121 atgcctcata tttatatgaa aaagacttta taaaaattta tttcgaaaaa agcaaaaaac
      181 agcaagcttt tgagaaaaat ctctcactgg cttactgttc ttttttgtgt ttacttacgc
      241 gacaattgct tgtaacggtt ataccaaata tcaatatagc cttccgagaa cgggccatct
      301 ccattattga tccagtcaac cagaatcttc acattttctt ttaggatgaa gtcaatatcg
      361 ttcgggtaat tcatcatttt gctgtgcatc tcatattcat cgacatccaa tagtcgtttc
      421 tcgccgtcag gaaatacctt gatatccaag tcatagtcga tatatttcaa ggcctcatcg
      481 tccagcaaaa aaggtgaagc tagattacaa taataggaaa cccccttttc tctgatcatt
      541 gctattacat tgaaccagta ttttttatga aaataaacaa tcgctggttc gcgcgttacc
      601 catcggcgtc catcagattc agtgactaat gtgtgatcat tcacgccgat catagagtgt
      661 tcgcttgttt ttaataccat ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg
      721 taactttgga tcgtcacaaa ctctccttct tttggaactc ccatagcaac ccttccttaa
      781 gcagcatttt gtgctttgtg tttgggtgtt cattacaccg acatcataat tattatatca
      841 ataaaaatga gctttgccta gaaaggattc ctattgtttc gatttttctc taactgatct
      901 accagtttca tttggacctt aggaaaaacg accgaatcaa atgcatcagg cttcaaccac
      961 ttcgaagttt tgttctcggt aaattcttgt tctgctccct cggttgcccg tccgtaaaaa
     1021 aggagaacat gccatttcag atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc
     1081 tgccaaacaa agagttcctg cttttcaaat gggagatttt gctcttcttc tgctactagg
     1141 tcatcaaaca gatcgatttc tggctttgtt tcccattctt ttttcaatcg ctcatactcc
     1201 tcttgtgtca cttccatcat tggaaatgtc cacatattcg ccagcaattt ttggctatct
     1261 ctttcttcaa aataatacgc tccagaatga ttttgaagag ctgcactgat ataataaacg
     1321 tcttttggct ttgctttttt tgtttttaca ggaaaactcg tttggattcc tcgtttgtta
     1381 gctaaacaaa aggcttggat cggacaagtc tcacactttg gagaagttgg tgtacagata
     1441 gctgaaccta gatccatcat tgcttggttg aattcacctg gatgcttttc atcaatgatt
     1501 ttccgcatcg cttcatcaaa gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg
     1561 caaaacaatc tgctgactac tcgcatcacg ttgccgtcaa ctgcaggttc tggaagtcca
     1621 aatgcgatac ttgcaatcgc gcctgtcgta tatggtccta ttcccttcaa tgagctaatt
     1681 tcttcaggcg tttgcggcat ttccccatca aattctgaca tgatctgttt agcagcagct
     1741 tggatgttcc gggctcttga gtaataacca aggccctccc atgcttttaa aagcttttct
     1801 tctggagcat ttgctagctc ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa
     1861 tcgatcactg tgtctactct ggtttgctga agcatgatct cagagatcca gatacgataa
     1921 ggatcacgat tatagcgcca aggcaggttt cttttttctt gttcatacca ttgaatgaac
     1981 tgatcttgaa attcttttgt ttctttatct gtccattttt cccattcttt attcactttc
     2041 ctcttccttt tccgtaatga atcgctggat catatccaaa tcaaatcctt tttgatataa
     2101 gctttgcttt acttttagat ttcttttttt aggttcaaac cttaaatttc ttcgccatag
     2161 tttatctcct tgtttgacta aataatcata ttctatttct tgatctgctt cttgcggaag
     2221 atcagctatc gcttgttgga tcacttcttg agaaaagcct ttcgtcatca atgtttgttg
     2281 gatttttctc aataattctt tttggctttt tccgtgattt tttccaaacg ctttttcggc
     2341 tgtctgatgt gcattttgaa cttggtcttc aaatgtatat tggctgagcg cttgttcaat
     2401 gatttcaggt ttcaaacctt tttgctgcat ctgctgcgct aatttctttg gtcctttgtc
     2461 acttaagcgc atattcgtgc gcacataact ttccccatag accaaatcat cgacaaggtc
     2521 caattctttt aagcgggcta cgattttatt tcgatcttct tgaaggattt ccttttcttt
     2581 taagtatgtt cggatttctt tttccgatcg caactgataa cttaagtaat tcattgctag
     2641 gtgaaaaccg aaatcagctc cgctactctt cttgatctct tggatagttt cttcgtctaa
     2701 ttcccgtcct tttaataatc gatagcgtac taatacatct tcagatactt gtaaagcttc
     2761 tccttcagat aaatctacgc gatagaaagg gcctttgcct ttactgattc tcgtaacggt
     2821 taacataaac atcctcctcg aacatatatt ctctatcata ccacagaaaa tccccgtaat
     2881 caggtattat atgttagaat aaaccacaga aaaaagaaaa agggagatac ttatggcaga
     2941 aacaattgtc aaaactggac aaacggttcc tttgaaaatc aagcgtttag gcataaatgg
     3001 tgaaggaatc ggttatttca aacggttgat catatttgtt ccatatgcac taccaaaaga
     3061 agaagtacta gtgaaaatca cgaaagcaac tcctcgctat gcagaaggag aattaataaa
     3121 agtcaaaaaa acaagcaaag atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg
     3181 tggctgtcaa ttacaacact tagcttatca agctcaattg gattttaaaa aggatctgct
     3241 tttgcaagca ttggaaaaat tcaaaccagc aggctttcga tcttatcaac ttttgccgac
     3301 gatcgggatg gatgagcctt ggcattatag aaacaaagca caatttcagt tacgaaaaaa
     3361 taagcagact caaaaaattg aagctggttt atacgaagca aactctcatc agttagtccc
     3421 tataacagac tgcttggttc aagaacctcg aacacaaaaa gtaatgaata ctgttgttca
     3481 actattgaat aaatttgatg cacccatcta tgatgaacgg acaaacagtg gaatcttccg
     3541 cacaatcatg gttagaatcg gtgttaaaac tggggagtta caagttgttt ttattactcg
     3601 cagtaaaaaa ttcccgcaaa aaaacgcaat gatccgtgag atcaatgaac gactgcccga
     3661 agttgtttca atcatgcaaa atgtccaggc acaaaaaacg tctctcgtga tgggggatga
     3721 aacgatccac ttatggggaa aagaagccat tgaagaacaa atcaatgaag tgactttcga
     3781 tctttctcct cgtgccttct ttcaactcaa tcctcagcag accgaattgc tttatggcga
     3841 aggaatcaaa gcattagatg tccagcctaa tgaaacgatc gtggatgctt attgcggagt
     3901 tgggacgatc ggcttaagtg tagcaaaaca agccaaagaa gtacggggaa tggataccgt
     3961 tcctcaagca atcgctgatg caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg
     4021 atatgaagtc gggacagcag agcaagttct gcctaaatgg ttgaatgaag gttttcagcc
     4081 tgatggtatc atcgtagatc caccacgtac gggtttggat aacgcattga tccaatcatt
     4141 attgaaatac cctccaaaaa aacttgttta tatctcctgt aatgtttcta cacttgccag
     4201 agatctggtt aatttaagta aaaagttcaa gattgaatat ttacagtccg tcgacatgtt
     4261 tcctcagact gcacgttgtg aagttgtcgt gaagatgacg agactcgctg actaaatgaa
     4321 agaggttgtg aaaaagtctg ccctgtatca agtaataaga acagccaaac aaaaatagct
     4381 cttcatgttt tatgaatact gtacttgtga ccgaagagtt aacaggagga cactatttat
     4441 ttaaaaaaag ggttgtgaca tagtttttgt cacagccctt tttgattatt attggatatt
     4501 cacgtctatc acttgataaa atgcatttgt cgtatcgctg atattccata ctgctaaaat
     4561 cacattatat ccttttcgat cctgcggaag tgtgatcgtt tggttgactg tttttggtgg
     4621 aacagttgct ttgtcgtcta tttttgtgat caattcaaaa tcactaaatt tcaaaggttg
     4681 attaggattc caagttggtt tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc
     4741 tgttaaattc caagtaatat ttaatgttcc tggattaacc atgtttttat gccatctgtt
     4801 agatgtctgt tcatctaaag gctcaaagcc gctgactcct gcactagcaa tttttccatc
     4861 aatgaatgta tttttggtag cttcgatact ttgcggttcc cattgtgctc ttccgacatt
     4921 ttgatttaag tttcctgctg ctgatgttcc taggtatgca cggcttcctg gagaagtaat
     4981 ataaccatgc gcaaaacctg gaatgctaaa accgaacaac cctaaactaa ctaacccaat
     5041 aaacaataac gtactaatct ttttcatgta gaaacctcct tcatttgata accacattgt
     5101 aaacgcttta ttgtattaac acaaacatta tgcttttata attttatgtt gaagtaaaaa
     5161 aggtgaaaaa agtaagatgc agattataac agaagtagac gagaaaagta caaatagaaa
     5221 acatgagaat caaatcatat gatctttttc aaaaacatca ttcatataaa caaatgttca
     5281 actttttaat aacaacaatt atcaattttt ttcttacttc acaaactaaa tacaggtctt
     5341 gctgtgagcc ttcatggtaa actaatatta tagaaaggag ttagtaaaat gaagatgata
     5401 aaagatattc gtttttgggt ttgtgttata ggaattgtca tattaggctt tttatcaggg
     5461 ttgcttagcg gaaatcccgg aaaatattac tattctttgc aacttccacc atttgcacca
     5521 cctagctgga tatttgggcc gatgtggacc ttactgtata tattgatggg tatttctctt
     5581 tatttgttac ttaatcacaa caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg
     5641 attcagttca tttttaattt tatatggtct gctctatttt ttaatttaag aaatattttc
     5701 attgcagcaa tcgatattac acttttagtt attttcttgt cagtgcttat gtatcagttg
     5761 tggctgcatc atcgcctgac aatgtggctc atgattcctt attatttatg ggtactattc
     5821 gcaactctat tgaattattc aatttatttt ttaaactaag cgctaaaaag agacgtcctg
     5881 taatttaaca catagaaaaa gtgccaccta agttagattt tttgtctaat ttaggtggca
     5941 ctctttatta accaaactat ttttttctag tacccacttg ttgttccata ctcattattc
     6001 tggtcataac cataaccatt ttgttcctgt ccagcgtaag gatcataact ttgagtatct
     6061 tgacttggtg tatagtactg acttgtatct tctgtttgct gataataggt attcggatct
     6121 tggtactgat catatgtgtt ttctgaatag gaagaataat cggtgtaccc ttcactattc
     6181 gatccatcac cgtttgtcga atcgccatct tccgaatcat caaagaacat cattgaagca
     6241 ttctcatttt tcaaattagg taatgtatcg cttgttttta tatttaattg tttcttcagt
     6301 tcattttgga tcgaaagaag atcatttttt cctaaaattt gatagtagat atcattgatc
     6361 atttgtcctt cgccttgcaa ctggtcttgt ttgattgttt caaacgccgg ggtgtagttc
     6421 gtcgctacat caagcatgtc atcccaatct aagtccgtcg tcacattgtt ctcaactgct
     6481 tttaaaattt ttctataatt gcttacgcca tctaaactta aaactttatt gacgattttc
     6541 gtcactactt cccgctgacg acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc
     6601 attcgagaat aggctaaacc tttttctcca ttcaatgttt gttcacctac tggtacatgg
     6661 acaccatcca aagtaaagtc aattttatta ttcactttga tgccaccgac cgcattgatc
     6721 aaatcttcca tgccatccaa attgatcgtt acataatgat caataggaat atcgagcagt
     6781 tgctcaattg aatccatggc catttcgaca ccaccaaatg cgtaggcatg atttaattta
     6841 tctaccgttc cataacctac gatattcgta taaatatctc ggtccaaact gatgatcgtt
     6901 gatttttttt gtttcggatt aactgttact accatcatcg tatcagaacg tccttgttcc
     6961 gttcttccta atccaccggt atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca
     7021 tcaatgctta ctctctcaga acgtttggag gatattcgat tagagttttt agaaattttg
     7081 tttactgttt ggctcgcttc tcccataaat ctgattccgt atgctgataa tccggcaatc
     7141 agcaaaacga atacccctaa caaggttaat agaacttttt gccatcgctt cataatacac
     7201 ctctgtttca cattttctaa aatattgtct catataactg ttcaatcagc aaattcactt
     7261 tttaatcata agaaaacttt aaaaaacatt tttgtgtaac gattattatt ttttaaaatt
     7321 tctcaaaatt cttaactatc ataccatacc gtgaaaaaga tcgaaagacc gaatacacgc
     7381 agttcgcaat cgttttatct ttttttatgt attttttcca gaaaaagcga acaacaaggt
     7441 attttccaaa taatcgtgca gtaaaatgat cgcacatacc tttcccatct ttttaacagt
     7501 ctttgcctac cgttcagatt atcattcgat ttcacgacaa tttcttccta tgactggtcc
     7561 ttttgttaaa cccatcttct attcgtttta tttccagctc actgcatggc tcttaaaatg
     7621 actcgaacta gtgattgcat taccatcatc ttttaatgga tagatatgct cctcttcaaa
     7681 gtaaaacgat tttcttttaa agtaatagaa ttgacatggt aatgagtatg tatactagtg
     7741 gtttcacaaa tggtctttta ttcaacatcc aaaaactgaa atttctttaa aatccattac
     7801 taacgaagaa aatgttcctt gcttgataat tagttaggac atgtcaagtg aacaaatgca
     7861 tttacttagc caaaaaatct aaaaacttca taaatagaca aaaagacacg actctctaag
     7921 aagtcgtgtc tttattcaac aaattattat aatttagtca cttcagcagc ttgaggtccg
     7981 cgtgcacctt caacaatcgt gaattctact tcttgacctt cttctaggct cttgaagccg
     8041 tctccttgaa ttgctgagaa atgaacaaat acgtcatctc cgccgtctac tgtaataaac
     8101 ccaaatcctt tttcattgtt gaaccattta accgttccgt gttccataaa tgtatatcct
     8161 ccaaaaaagt tcttgcaagt aaccttgcaa gaacattata cccaatttag aaaacgctag
     8221 caagctttcg ctcttttatt ttccaaatta tttttaagac tgatatgacg gaggaaatgg
     8281 ttattttgct gcttcgaaac gtttgtttac ttcatcccaa ttaataacat tccagaatgc
     8341 tgcaatataa tctggtcgga catttttata tttcaaataa taagcatgtt cccaaacgtc
     8401 caaacctaga attggtgtct ttccttccat caatggagaa tcttgatttg cagtagaggt
     8461 aatagctaat tttccatttt ccattacaag ccaagcccat ccagaaccaa atcgtccagc
     8521 ggctgctttt ttgaattctt ctttaaaaga agaaaaatca ccaaaagctt cattaatcgc
     8581 ttcttttatt tctcctgtag gttcgccacc agcatttggt gccatgattt cccagaaaaa
     8641 tgaatggtta gcatgtccgc caccattgtt acgtacagct gtcttgatat ctgttggaat
     8701 agcgtccata tcagataata attcttctat tgttttttcg cctaattctg ggtatttctc
     8761 aattgctgaa tttaaattcg tcacataagt attgtgatgc ttatcatgat gcagatgcat
     8821 cgtttcttcg tcaatataag gttccaatgc gtcatatgca taaggtagat ctggtaatgt
     8881 gtaagtcata aaaaaattcc tcctcaatgt tttcttaata atgtctacat tacaaagagt
     8941 aacaaagaga aggaaattct tcaattgtta tgcctagtcg tctttttcac tttgacctac
     9001 ttcttcttca aaaacctcag tttctttaaa aataaacaac ttgctgaaaa tatagttcgc
     9061 aaggataaca ataatttgag tgatgatttt cgccacatac tcattgctgt tcataacatt
     9121 gatcaacaat accattgccc ccatgtccaa tccgtaagaa aggatacggt agaagaagaa
     9181 cttaccgcat tcaataagtg catgtgtaaa attcggtgat tttgaatgga atacccaaag
     9241 tttgttcgtt acaaaagcaa acaatacaga acaaaaccac gaaatagaat tggataaggc
     9301 catacttaaa tcaaaatatt ggcgtgcgag agcaaaacta acaaaattta caatcgtagc
     9361 caaaccacca aaaaagagat agactagtat ctcccaatat ccttttgctt ctaaatatcc
     9421 ttttaactgt cgatactgct tcatgagcca acctacctta ttaaaaattc ttatttatca
     9481 tactctatat ttcaataact ttcatcttta tttaaaaaaa taaatttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt aataatagta
    20221 taaaatttct tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata
    20281 ctgaaaaaga tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat
    20341 ttaaaggagt attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata
    20401 ttatacccat tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta
    20461 gtataattaa acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta
    20521 tacttccaat caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat
    20581 aaggtctatt cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg
    20641 aaagaagtaa agtaactata gcagctttta ttaatttaat aattaagaag atttctattt
    20701 tctttttccg ataaaattga ataattaaag caattataat cattaccata gtcatagcta
    20761 cagaaagaac atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt
    20821 ctttttttga gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg
    20881 cgagtaattc ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg
    20941 tatatataat a
//
LOCUS       DACDTH010000049        20549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDTH010000049 DACDTH010000000
VERSION     DACDTH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.049"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(179..898)
                     /locus_tag="IWK54_001920"
     CDS             complement(179..898)
                     /locus_tag="IWK54_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9594037.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1029..2180)
                     /locus_tag="IWK54_001921"
     CDS             complement(1029..2180)
                     /locus_tag="IWK54_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9594038.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2397..3137)
                     /locus_tag="IWK54_001922"
     CDS             complement(2397..3137)
                     /locus_tag="IWK54_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9594039.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3141..3464)
                     /gene="rsfS"
                     /locus_tag="IWK54_001923"
     CDS             complement(3141..3464)
                     /gene="rsfS"
                     /locus_tag="IWK54_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9594040.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(3521..4114)
                     /locus_tag="IWK54_001924"
     CDS             complement(3521..4114)
                     /locus_tag="IWK54_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9594041.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4098..4745)
                     /locus_tag="IWK54_001925"
     CDS             complement(4098..4745)
                     /locus_tag="IWK54_001925"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9594042.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4758..5069)
                     /gene="yhbY"
                     /locus_tag="IWK54_001926"
     CDS             complement(4758..5069)
                     /gene="yhbY"
                     /locus_tag="IWK54_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9594043.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5085..6194)
                     /gene="yqeH"
                     /locus_tag="IWK54_001927"
     CDS             complement(5085..6194)
                     /gene="yqeH"
                     /locus_tag="IWK54_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9594044.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6191..6718)
                     /locus_tag="IWK54_001928"
     CDS             complement(6191..6718)
                     /locus_tag="IWK54_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9594045.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6898..7683)
                     /locus_tag="IWK54_001929"
     CDS             complement(6898..7683)
                     /locus_tag="IWK54_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9594046.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(7676..8545)
                     /locus_tag="IWK54_001930"
     CDS             complement(7676..8545)
                     /locus_tag="IWK54_001930"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9594047.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8546..9925)
                     /locus_tag="IWK54_001931"
     CDS             complement(8546..9925)
                     /locus_tag="IWK54_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9594048.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9933..10352)
                     /gene="fabZ"
                     /locus_tag="IWK54_001932"
     CDS             complement(9933..10352)
                     /gene="fabZ"
                     /locus_tag="IWK54_001932"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9594049.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10377..10853)
                     /gene="accB"
                     /locus_tag="IWK54_001933"
     CDS             complement(10377..10853)
                     /gene="accB"
                     /locus_tag="IWK54_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9594050.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10856..12091)
                     /gene="fabF"
                     /locus_tag="IWK54_001934"
     CDS             complement(10856..12091)
                     /gene="fabF"
                     /locus_tag="IWK54_001934"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9594051.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12110..12847)
                     /gene="fabG"
                     /locus_tag="IWK54_001935"
     CDS             complement(12110..12847)
                     /gene="fabG"
                     /locus_tag="IWK54_001935"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9594052.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12859..13776)
                     /gene="fabD"
                     /locus_tag="IWK54_001936"
     CDS             complement(12859..13776)
                     /gene="fabD"
                     /locus_tag="IWK54_001936"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9594053.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13883..14107)
                     /locus_tag="IWK54_001937"
     CDS             complement(13883..14107)
                     /locus_tag="IWK54_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9594054.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14160..15122)
                     /locus_tag="IWK54_001938"
     CDS             complement(14160..15122)
                     /locus_tag="IWK54_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9594055.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15132..15572)
                     /locus_tag="IWK54_001939"
     CDS             complement(15132..15572)
                     /locus_tag="IWK54_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9594056.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELAITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            15878..16843
                     /locus_tag="IWK54_001940"
     CDS             15878..16843
                     /locus_tag="IWK54_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9594057.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(16900..17130)
                     /locus_tag="IWK54_001941"
     CDS             complement(16900..17130)
                     /locus_tag="IWK54_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9594058.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17216..18103)
                     /locus_tag="IWK54_001942"
     CDS             complement(17216..18103)
                     /locus_tag="IWK54_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9594059.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18192..19415)
                     /locus_tag="IWK54_001943"
     CDS             complement(18192..19415)
                     /locus_tag="IWK54_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9594060.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(19432..20511)
                     /locus_tag="IWK54_001944"
     CDS             complement(19432..20511)
                     /locus_tag="IWK54_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9594061.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         5547 a         4565 c         3484 g         6953 t
ORIGIN      
        1 attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact tatgctctca
       61 tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa gacaatctgt
      121 tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa ctggttgctt
      181 agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct tccagtgcat
      241 caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg ttttgaggga
      301 tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat gcttcttgta
      361 tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct tccattacat
      421 cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg tcttctcctg
      481 caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa acacccatgt
      541 tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc acattgttcg
      601 tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag cgcaattcag
      661 agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata atatgttttg
      721 gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt gcatgcggat
      781 caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca taaatccggc
      841 tattattagc atctttagca gttttctttg ctacgatatt tgcccattta cgtcccatat
      901 tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt tccattatac
      961 ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc tcatttttag
     1021 tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat attgcctccc
     1081 aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc tttttttccg
     1141 attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc agcatcggta
     1201 aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg ctttttgata
     1261 ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct ttttgttttt
     1321 acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt catgcgcgat
     1381 tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga agataacaac
     1441 cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt tgaaagatct
     1501 tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa cgcatggcga
     1561 atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata gcctgcactt
     1621 ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc ctttgcataa
     1681 cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg atacagactg
     1741 cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag ttcttgaaat
     1801 gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct gtaatcaaac
     1861 gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg caagattttt
     1921 actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg aagctccact
     1981 actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc gatgatagcc
     2041 ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc tgctcctgtt
     2101 atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt gtactctacg
     2161 ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa aagaaataga
     2221 gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac tattctcgca
     2281 aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata tcgttcggat
     2341 atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca aaaccgctag
     2401 tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc gtctgtaaaa
     2461 tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa ataactttcc
     2521 aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc agacccttct
     2581 tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct tcctggataa
     2641 ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc tggaaaaacc
     2701 tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc ttctaacgct
     2761 tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata gcacagcgaa
     2821 tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag catattgcct
     2881 tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat catcaacatc
     2941 tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc aaccgctaat
     3001 gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa atgacgtttc
     3061 gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta aatgaaagca
     3121 aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag cggagcatct
     3181 gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac atgtacgatc
     3241 acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat acgttttact
     3301 tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt gatttgacgt
     3361 tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt tcgaacgtct
     3421 aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat ttttaattgt
     3481 tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt gctacccagt
     3541 aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc aaatgggcca
     3601 atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca atggctcgtg
     3661 catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca taaatgattt
     3721 tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca tgaagaatct
     3781 ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga ccatgccaga
     3841 ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt accattttga
     3901 actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata ctagcttttt
     3961 caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact ccaagcacat
     4021 gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt tctctagaat
     4081 aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg aatataatca
     4141 attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt ttgacgtatt
     4201 ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata tggagtatcc
     4261 gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag ctcatcgatt
     4321 ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta ataatctgta
     4381 tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt cccaccacga
     4441 gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa ttccaacatg
     4501 ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg ctgataagaa
     4561 ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc catcactaaa
     4621 tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt tttcttctct
     4681 aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt agcttgcgta
     4741 ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg atacttttct
     4801 tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat ttcgcaatct
     4861 atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt ttgaagtaac
     4921 gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat cattgctgaa
     4981 ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt gctacgtaaa
     5041 aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat caatgctttg
     5101 cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg cgctgttaca
     5161 gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga aaactcaaaa
     5221 cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa taatccacct
     5281 acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat atttacatca
     5341 tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa tcttgcaagt
     5401 ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt gatttctttt
     5461 aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg atggcgatgg
     5521 ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc gattttatcc
     5581 aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc tgtatttttg
     5641 atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat atagacatcc
     5701 cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg agatttctta
     5761 gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg ttccttcatc
     5821 cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt gttcccaact
     5881 aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga accattgaag
     5941 tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc attcagtaag
     6001 cgcaaaaagt cgtcatccgt caatgagaca tcttgggtct cattataatg gcgtaatctg
     6061 aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc cgtttgcggc
     6121 gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc gatacattgt
     6181 aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt gctaatagat
     6241 aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta tctgttgcga
     6301 taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt atatcagtca
     6361 taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt ttctttgcta
     6421 tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga tcaaattttt
     6481 caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca gggattcctg
     6541 catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga ttattccatg
     6601 caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc tttaattggg
     6661 caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt gaaaacatgc
     6721 tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc ccgtattata
     6781 ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa aagcatttct
     6841 ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa aaaacaacta
     6901 atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta atcgaacaag
     6961 ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat atcgtggcaa
     7021 ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact cgcgtagttc
     7081 tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt ctttccacaa
     7141 aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca gcatccagac
     7201 ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa tgatgatggc
     7261 gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag cctctccttc
     7321 gccgcgctct tctgctccta caccgcaata tgcacctgct gtattgatca aggtaatcac
     7381 cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat agccttcagg
     7441 atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt tttgaatacc
     7501 gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg cttcgtcatc
     7561 tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt caaaaaaatc
     7621 caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg ctgtcttatc
     7681 cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt ttctgtttca
     7741 attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat tcagcctttt
     7801 ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt ccagcaaaac
     7861 cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa ctggcagtta
     7921 ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca ttgctatgcc
     7981 gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct tcttgcatcc
     8041 gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt gctcgttcga
     8101 ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata aaattaggat
     8161 ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg aggactgctt
     8221 catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg ggaaatgcta
     8281 gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca tcaataatca
     8341 aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg catacttttt
     8401 cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt gcccacatgt
     8461 tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg atccgaatat
     8521 atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct ggtgtccaat
     8581 tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg actgcaggat
     8641 gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg ataaattcac
     8701 ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg acgattattt
     8761 ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact gcactgtcta
     8821 ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct ggagaaggag
     8881 caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt ccttggaata
     8941 ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc tgttttttta
     9001 ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt cgagtattca
     9061 tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt ccggcatttt
     9121 cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct ctttttgttt
     9181 gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt tgtaaagaac
     9241 aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc tgaacttcta
     9301 tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca aatgccgctg
     9361 ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat tgaactttcc
     9421 gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa ccaattctgt
     9481 ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct ggaatgactg
     9541 ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt gcatcgatcg
     9601 ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag gcaaatcggc
     9661 tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc acgattgcag
     9721 cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg ccaatgcaga
     9781 ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa tatatcgcta
     9841 cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt tcaccgcggt
     9901 tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt taattccgct
     9961 tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc tgaagctttc
    10021 actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt cttacggaat
    10081 tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc aagagaaagt
    10141 aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg cataacaggt
    10201 tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt cttagcaacg
    10261 atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg ataacgatgt
    10321 ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg ctttgcttat
    10381 ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt ttctacaagg
    10441 atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt catcgcttca
    10501 ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa agctggtttg
    10561 tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc gattccatca
    10621 aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg ttcaggtatg
    10681 acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt catgcctcga
    10741 cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc gaattccgtt
    10801 agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc caaaattatt
    10861 cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat gaattactca
    10921 ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag tcaatctcag
    10981 gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat gccagcaaag
    11041 ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc gatttcgtgc
    11101 tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct tttgcttctg
    11161 cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact tgttccggct
    11221 gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa ccatctggag
    11281 taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt tctccataga
    11341 ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg cctgctcctt
    11401 cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga ttaggatcag
    11461 ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca caaatcgttg
    11521 cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta cggaatgctt
    11581 ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa ataccttttg
    11641 cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt ggtacgaaca
    11701 ttggagaaac tcgttttgca cctttttcat gtagacgaat aacttggttt tcgattgttg
    11761 gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc attttttctt
    11821 tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag atcgaaaaca
    11881 tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa tctttgactt
    11941 cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt gcgatgccat
    12001 tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata ggtgaagtga
    12061 ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct tatccgtgca
    12121 tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt tgtgctaaga
    12181 aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga atttgctttt
    12241 ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg atgaatcctg
    12301 gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca gattttgtta
    12361 atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt ccaatcaacc
    12421 cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt tttttcaata
    12481 cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt ttgaaatctt
    12541 ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt actaacacat
    12601 ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc tcataatcag
    12661 agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct tctatctttt
    12721 cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt gcaaaagcta
    12781 aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt tttcctttta
    12841 catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct gcttattctc
    12901 aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta atacttttcc
    12961 ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga tactctcata
    13021 gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt ctttcttcat
    13081 tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt gaatctgact
    13141 taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa aaggcccgct
    13201 gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa cagcttcgtc
    13261 tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat ttgcaggaga
    13321 tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa tcggcgcatt
    13381 catcactgca accattttcc cgcttccagc tggggcagct tctgtcatat aagcgcctct
    13441 ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta ctaatgccga
    13501 atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct ccttcaataa
    13561 gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg tctgatccaa
    13621 acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct cacttgcttc
    13681 atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac cttgatattg
    13741 tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata tatccagaat
    13801 atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg aaatgtccca
    13861 gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc actaaatctt
    13921 gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct tcaatttcat
    13981 tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg tcagtagtca
    14041 atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga attttttcga
    14101 ataccataat aatatattcc tccgatattg tttgcttttt atttttataa tttaacagtc
    14161 tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt aaatattact
    14221 ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa taagataggg
    14281 atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt ttcttgcggc
    14341 atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg caaaagcaga
    14401 taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc tcgtacggca
    14461 aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga atcttctgaa
    14521 tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct ggtaccgtca
    14581 gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag aatcccacct
    14641 gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag cttagatagt
    14701 gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt ttcagccatg
    14761 cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat agcatgagtt
    14821 gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc gggcgacatt
    14881 gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt tatcaatttt
    14941 tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct tcgttcacga
    15001 atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga aagatcatta
    15061 ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc aaattgttcc
    15121 attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag tgcatccagc
    15181 aaggcatgct gttcctccgt agccattccg tctaagatgt tcttgaccat ttcacgatga
    15241 aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa ctttacaacg
    15301 cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa cttgttaata
    15361 gcagtggtca aggtcccaac agtaattgct agttctttgg ctacttctga agttgttttt
    15421 ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag atctttgaat
    15481 tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac actaacaaga
    15541 taatcattta ctgtttttaa atctggttcc atgttccacc gtccactaat tagtttgatt
    15601 atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa ccttaaaaga
    15661 tcattttttt actatatgag ctttttttaa gattttactc atttaaaaca actgataaat
    15721 cagcatttat aatttctttt attagaaaaa tcatctatct cacatatttt gattatcaaa
    15781 gtattttctg tgaacagaac tatagaattc cctaaaactt gtccattatt tcaaaatttg
    15841 ttatagtctt tttattcaaa gcgtaaaagg agatcctatg ttaaaagaaa aaatgaaaac
    15901 tctccccatg ctgccgggtg tctatcttat gaaagaccga gatggtaagg tcatctatgt
    15961 tggtaaagca aaacggctca aaaatagagt aagcagctat tttcatcaga ataaacagca
    16021 ttcaaaaaaa gtattacgta tgattcatca tataacagat tttgatttcg tcgtagtaga
    16081 cacggaatta gatgctttat tgctcgagtg tcaattgatc caacattacc gtccttttta
    16141 taaccgacaa atgaattatt tcagtaatta taattatgta catattacca ataagggatt
    16201 tgtattaaca gatactccta cggctcgaac atacgggcct tttcgtctct ataaaaaaat
    16261 gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg ccttggttgt ctgaaattag
    16321 tttgttagct ttgcgagtcc agcttcctga cctacaggaa atgacttttg aacagaaaaa
    16381 aaaggaactc caagggctgt ttcaaggccg aaataaaaag ctactgactt acttgaaaaa
    16441 aagacaacaa catttcatct atcagttgaa tttcgaaaaa gccggtatgc ttcaaaaaga
    16501 tatcgaatta gttacttatt ttattagacg gatccaggaa caaaagcagt ttttaagaac
    16561 accatcgctc accttcagca tgccattagc agcagatgag agtcagaaaa agcactacct
    16621 cgtttgttat ggtcaacttg cagaaacaat gattacctct ggtgacgttt ctcctgactt
    16681 ctattatgaa aagaaagaag ctcatttatc tttgaaacgt caattgtcaa aagaagaaat
    16741 cgatcctgta caaatcttga tcagttaccg caaaaaatta gaaaaagaac agtttgaaat
    16801 tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat tagttgcttc tcggcttctt
    16861 ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt tattggatta agtcatagat
    16921 ttccatcgct acgatatcga tattatcaaa ttcgtattgc tgatgagtat ctgtttcagt
    16981 taattcaaat gtatcggtcg ctttatcata agttactgta cacttctcta cgccttcttt
    17041 ttcaaaacgg cgaacttgca gttcattatc tgctgactcg accatagcct ccagtcgttt
    17101 tatgattgcg actaattgtg aatgtgtcat cggaggtcct ccttttctta tttctgttat
    17161 attgtatcaa aagcatagcc tacttgcatt gattttctct aaaaaaactc gttttttatg
    17221 attttttttc tggtattttt ccttcaggcc accaaagttt gatcaaagct tgggttccgt
    17281 cattctcaaa acctttatct gccaactctt catatagttt caatgcttgg gtagttgctg
    17341 gcaatacaag atccattttt tctgcttctt ctaatgcaat ctttaaatct ttgataaaat
    17401 gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat acgaggagaa taattgctta
    17461 aagaccagtt agctgcactt ccacctgaaa gcgtctcaat cactttcttt aaatcaagtc
    17521 cgtttttttg tgcatagaca agcatttccg tcattcctgt catcgtacct gcaatcatga
    17581 tctgattagc catttttgta tgctgacctt tcccagcaga accatgcaat gtaaatgtcg
    17641 taccaaattc tttgaataaa ggcagtactt gatcataaac ggcttcttct ccaccgacca
    17701 tgatcgtcag tgtaccattc tttgctccta agtcaccacc ggataccggt gcatctaacg
    17761 catctgcgcc tttttctttt gctgtttttg caattttttc tgccaggctt ggtgtacttg
    17821 tagtcaaatc aactaaaata tgtcctgaga tatctgcaga aaaaatcccc gactgtccat
    17881 agtagatttc ttctacatct ttggggtaac ctaccattgt aaagagaata ttactttgtt
    17941 ctgctaccgc ttttggtgta tctgcccaga tagctccttc tgttactaaa ggatcagctt
    18001 ttgcttttgt ccgattatag acagctactt cgtgtcctgc ttctagtaga tggcgtgcga
    18061 cagcacttcc catgacgcct gttccgataa aaccaagttt cattttttcg acctcctaaa
    18121 aatcaaaaat ccttgcttct atgctagtat acgctagaaa caaggatttt ttcataaaaa
    18181 atgtttgaat tttatctttt aaatgaattt aattttattt tgattcgttc aatcgagtct
    18241 tcttcttctt ttcttcgcat ctcttcttca tgataaacaa ccatgtcttg ataaaattcg
    18301 atcatagctt cgccgaaatg tgtagatgaa atatcataaa gtttagcttc taaaactttt
    18361 ggatctttag gaacttcact ggaaatataa tcaataagtg ctggtgcaaa gtcttcgtcg
    18421 gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc ggttcaaata agcatttcct
    18481 tccacgacac aaggtgttcc ggctgccatc gcttctgtat aagtaagccc ttgtgtttct
    18541 gaagtagaag cactgacaaa ataatctgcc gctttataat aaagtgctac atcattgttg
    18601 tccacttctc ctgtgaactg cacaaattct tctaactgca gttctgagat caattcctct
    18661 aactcttcta cataaggtcc ttttcctacg ataactaatc tggcgttagg ataagcggag
    18721 atgacctgag gaaacccatt aatcaaggct tggatatttt tctcataaga aatcctgctt
    18781 aaagaaagaa tcatcaaatg gttttcctga agacctagtg actcccttaa tttgctgata
    18841 tcctgctgag tgatatcaga acgtttgaat ttttcaatgt cgataccagt aggaatcacg
    18901 cgaagcggtg ctattacacc atagctgcgt aatgtatcaa taacacgttc acttggacaa
    18961 actacacctg tcgtatgatt tgcaaataat ctagacaagt attttacatg ggattgcctg
    19021 atcacttttc ctttaccgat ataatgaagg tagtcttcat acatcgtatg gtaagtatgg
    19081 atcagaggta ttttcaactt cttagctacc atcttcccaa gtatccctgc tccgaattct
    19141 gtatgggtat gaataagatc tagttctagt tctttggcaa ccaaataagc ataccacatg
    19201 cctctaacaa caactcttcg atccttgaaa gaaataaatg gtacacttgg cattcgaatc
    19261 acatcttttt ccaacttatc cgcattagga tcggtcgttg taaaaatata gacttcatgc
    19321 ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg tagcaactcc gctgacttgc
    19381 ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt tcgcctccaa actagtcctt
    19441 gacctgattt acttttttta atttgctaga gactttttca ttcagtacac tttcgtatac
    19501 atttttaagt tcttcaccga ttttattgat acttcgctct tttgctgttt gataaccggc
    19561 ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg atcagtcgga taaattcttc
    19621 gtttgtcttc cccatatagc agttcttgct atcttctaac cagccattat acactggaat
    19681 atctcgaaca agtacgtttt gtttgcttgc taaagcttct aaaacaacga tcccttctgt
    19741 ctcttcataa gaagggaaaa agaatagatc tgcacctgaa tacgcacctt ctatgatctc
    19801 tccacgaatg taaccaggaa acaggacatt ttcaggatga tcttctttta caattttccg
    19861 gatgttccga ggaatcgagt acatgggaac atgaccaaac caaataaatg tgtattcggg
    19921 catcttctta gcaatttcta cgaaatcaat gattccttta cgttcgaaaa acaaaccgac
    19981 acaaatgatt actttttgat ccggagacag tttgaaatat tctctgaact tttgttcttt
    20041 cattggatca gggtgatatt tctctaagtc gatcccattg gaaatagctt gaatcggcac
    20101 acggatacca tagctttcca acaactgttt tgaataaggt gttggcgtga tcagatagtc
    20161 cgctttttgg tacaatccta ccagatattt ttttacgata ggagaaagct ggttcgatcc
    20221 gataaaggaa tttcggaaat cttcctctgt agaatgcgca tgatagacaa ctttttttcc
    20281 ttctcttctg gctttattga ccatattatg actattgatt ccgtatgtat tgatgtgcag
    20341 gatgtcataa tcttcttttt catctagtgt ataagagata ccgacttccg tcagagcacg
    20401 tttttgatga tcaagcgcac gcccgattcc agattttgca agcatctttt cactttcaaa
    20461 atataataat accttcacct tacgtccccc ttcacttata tagtataaca tagcctacaa
    20521 actgtcactc aaacttaagc cctattttt
//
LOCUS       DACDTH010000050        20484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDTH010000050 DACDTH010000000
VERSION     DACDTH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.050"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            3..551
                     /gene="hslV"
                     /locus_tag="IWK54_001945"
     CDS             3..551
                     /gene="hslV"
                     /locus_tag="IWK54_001945"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9594062.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            565..1965
                     /gene="hslU"
                     /locus_tag="IWK54_001946"
     CDS             565..1965
                     /gene="hslU"
                     /locus_tag="IWK54_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9594063.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1983..2774
                     /gene="codY"
                     /locus_tag="IWK54_001947"
     CDS             1983..2774
                     /gene="codY"
                     /locus_tag="IWK54_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9594064.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2856..3731
                     /locus_tag="IWK54_001948"
     CDS             2856..3731
                     /locus_tag="IWK54_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9594065.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="IWK54_001949"
     CDS             complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="IWK54_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9594066.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4628..5062
                     /locus_tag="IWK54_001950"
     CDS             4628..5062
                     /locus_tag="IWK54_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9594067.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5228..7273
                     /gene="parE"
                     /locus_tag="IWK54_001951"
     CDS             5228..7273
                     /gene="parE"
                     /locus_tag="IWK54_001951"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9594068.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7285..9735
                     /gene="parC"
                     /locus_tag="IWK54_001952"
     CDS             7285..9735
                     /gene="parC"
                     /locus_tag="IWK54_001952"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9594069.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10012..12243
                     /gene="pflB"
                     /locus_tag="IWK54_001953"
     CDS             10012..12243
                     /gene="pflB"
                     /locus_tag="IWK54_001953"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9594070.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12487..13248
                     /gene="pflA"
                     /locus_tag="IWK54_001954"
     CDS             12487..13248
                     /gene="pflA"
                     /locus_tag="IWK54_001954"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9594071.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13333..14259
                     /locus_tag="IWK54_001955"
     CDS             13333..14259
                     /locus_tag="IWK54_001955"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9594072.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14441..15334
                     /locus_tag="IWK54_001956"
     CDS             14441..15334
                     /locus_tag="IWK54_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9594073.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15468..15593
                     /locus_tag="IWK54_001957"
     CDS             15468..15593
                     /locus_tag="IWK54_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9594074.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15727..16359)
                     /locus_tag="IWK54_001958"
     CDS             complement(15727..16359)
                     /locus_tag="IWK54_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594075.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16507..17049)
                     /locus_tag="IWK54_001959"
     CDS             complement(16507..17049)
                     /locus_tag="IWK54_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9594076.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17184..17573
                     /locus_tag="IWK54_001960"
     CDS             17184..17573
                     /locus_tag="IWK54_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594077.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17577..18341
                     /locus_tag="IWK54_001961"
     CDS             17577..18341
                     /locus_tag="IWK54_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9594078.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18470..19417
                     /locus_tag="IWK54_001962"
     CDS             18470..19417
                     /locus_tag="IWK54_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9594079.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19547..20287
                     /locus_tag="IWK54_001963"
     CDS             19547..20287
                     /locus_tag="IWK54_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594080.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7192 a         3481 c         4316 g         5495 t
ORIGIN      
        1 acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat
       61 ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg
      121 caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg
      181 cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc
      241 tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac
      301 ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag
      361 aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg
      421 ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag
      481 aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag
      541 aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta
      601 agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct
      661 ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca
      721 aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg
      781 gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt
      841 tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc
      901 gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt
      961 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
     1021 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
     1081 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
     1141 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
     1201 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
     1261 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
     1321 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
     1381 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
     1441 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
     1501 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
     1561 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
     1621 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
     1681 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
     1741 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
     1801 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
     1861 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
     1921 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
     1981 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
     2041 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
     2101 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
     2161 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
     2221 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
     2281 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
     2341 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
     2401 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
     2461 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
     2521 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
     2581 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
     2641 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
     2701 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
     2761 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
     2821 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
     2881 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
     2941 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
     3001 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
     3061 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
     3121 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
     3181 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
     3241 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
     3301 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
     3361 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
     3421 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
     3481 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
     3541 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
     3601 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
     3661 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
     3721 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
     3781 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
     3841 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
     3901 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
     3961 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
     4021 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
     4081 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
     4141 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
     4201 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
     4261 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
     4321 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
     4381 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
     4441 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
     4501 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
     4561 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
     4621 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
     4681 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
     4741 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
     4801 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
     4861 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
     4921 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
     4981 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
     5041 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
     5101 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
     5161 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
     5221 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
     5281 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
     5341 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
     5401 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
     5461 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
     5521 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
     5581 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
     5641 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
     5701 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
     5761 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
     5821 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
     5881 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
     5941 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
     6001 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
     6061 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
     6121 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
     6181 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
     6241 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
     6301 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
     6361 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
     6421 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
     6481 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
     6541 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
     6601 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
     6661 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
     6721 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
     6781 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
     6841 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
     6901 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
     6961 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
     7021 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
     7081 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
     7141 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
     7201 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
     7261 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
     7321 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
     7381 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
     7441 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
     7501 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
     7561 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
     7621 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
     7681 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
     7741 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
     7801 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
     7861 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
     7921 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
     7981 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
     8041 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
     8101 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
     8161 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
     8221 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
     8281 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
     8341 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
     8401 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
     8461 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
     8521 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
     8581 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
     8641 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
     8701 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
     8761 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
     8821 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
     8881 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
     8941 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
     9001 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
     9061 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
     9121 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
     9181 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
     9241 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
     9301 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
     9361 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
     9421 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
     9481 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
     9541 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
     9601 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
     9661 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
     9721 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
     9781 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
     9841 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
     9901 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
     9961 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    10021 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    10081 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    10141 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    10201 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    10261 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    10321 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    10381 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    10441 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    10501 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    10561 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    10621 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    10681 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    10741 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    10801 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    10861 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    10921 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    10981 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    11041 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    11101 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    11161 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    11221 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    11281 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    11341 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    11401 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    11461 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    11521 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    11581 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    11641 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    11701 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    11761 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    11821 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    11881 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    11941 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    12001 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    12061 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    12121 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    12181 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    12241 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    12301 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    12361 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    12421 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    12481 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    12541 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    12601 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    12661 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    12721 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    12781 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    12841 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    12901 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    12961 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    13021 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    13081 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    13141 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    13201 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    13261 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    13321 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    13381 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    13441 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    13501 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    13561 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    13621 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    13681 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    13741 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    13801 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    13861 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    13921 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    13981 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    14041 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    14101 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    14161 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    14221 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    14281 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    14341 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
    14401 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
    14461 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
    14521 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
    14581 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
    14641 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
    14701 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
    14761 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
    14821 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
    14881 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
    14941 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
    15001 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
    15061 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
    15121 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
    15181 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
    15241 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
    15301 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
    15361 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
    15421 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
    15481 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
    15541 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
    15601 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
    15661 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
    15721 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac
    15781 acctagactg acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg
    15841 gttggtcagt ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt
    15901 gacaggaact aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat
    15961 aaaataagta aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt
    16021 attgtgatta aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa
    16081 tacgcttgaa gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata
    16141 aacagaaaac agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc
    16201 ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga
    16261 ggtcttaagg tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc
    16321 taaatgtaaa aaattagtgg ttctacgttg aatttccata atatctccaa attaaattga
    16381 taagtatata aagtataata acaaattgat gagtatatgt cgatgttttt taatcaaaat
    16441 aataaataaa aactgtctaa catttaaaat ttaatttaga tattagacag tttttaagtt
    16501 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
    16561 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
    16621 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
    16681 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
    16741 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
    16801 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
    16861 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
    16921 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
    16981 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
    17041 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
    17101 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
    17161 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
    17221 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
    17281 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
    17341 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
    17401 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
    17461 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
    17521 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
    17581 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
    17641 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
    17701 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
    17761 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
    17821 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
    17881 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
    17941 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
    18001 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
    18061 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
    18121 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
    18181 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
    18241 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
    18301 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
    18361 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
    18421 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
    18481 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
    18541 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
    18601 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
    18661 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
    18721 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
    18781 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
    18841 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
    18901 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
    18961 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
    19021 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
    19081 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
    19141 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
    19201 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
    19261 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
    19321 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
    19381 ttttcctgaa ctagttatca gagagacaac gaattaagtc ttattactat atatatattt
    19441 atgaaattca tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc
    19501 tgtgctatta ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag
    19561 acgaaaacat ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga
    19621 caagaagcga gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc
    19681 gtttgaacag atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa
    19741 gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa
    19801 aggaaaatta ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa
    19861 aaaacttttt ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa
    19921 gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc
    19981 taaatatgta cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca
    20041 tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg ttgttcgata cagaaccgac
    20101 gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac
    20161 gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga
    20221 acgctcatta caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt
    20281 taaataagtg atatacaaat ttatattcgg ctacagttgg aaaggaaacg ctgcggcgtt
    20341 tccttatttt atactctatc cgaaagttta tatcgtatcc tcaaattttt ttgagtttaa
    20401 gcgaattttt tctcccaata agtaagaaaa taatggcaaa ataaaaaaac aaactatttt
    20461 cgcagatcgt ttgcaaaaat agtt
//
LOCUS       DACDTH010000051        19997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDTH010000051 DACDTH010000000
VERSION     DACDTH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.051"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..626)
                     /locus_tag="IWK54_001964"
     CDS             complement(<1..626)
                     /locus_tag="IWK54_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9594081.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="IWK54_001965"
     CDS             899..3370
                     /locus_tag="IWK54_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9594082.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="IWK54_001966"
     CDS             complement(3473..5209)
                     /locus_tag="IWK54_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9594083.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="IWK54_001967"
     CDS             complement(5206..5910)
                     /locus_tag="IWK54_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9594084.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6544
                     /locus_tag="IWK54_001968"
     CDS             6041..6544
                     /locus_tag="IWK54_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9594085.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6504..6842
                     /locus_tag="IWK54_001969"
     CDS             6504..6842
                     /locus_tag="IWK54_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9594086.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6863..8281
                     /gene="ffh"
                     /locus_tag="IWK54_001970"
     CDS             6863..8281
                     /gene="ffh"
                     /locus_tag="IWK54_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9594087.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8393..8971
                     /locus_tag="IWK54_001971"
     CDS             8393..8971
                     /locus_tag="IWK54_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9594088.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8975..9496
                     /locus_tag="IWK54_001972"
     CDS             8975..9496
                     /locus_tag="IWK54_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9594089.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9575..10129)
                     /locus_tag="IWK54_001973"
     CDS             complement(9575..10129)
                     /locus_tag="IWK54_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9594090.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10382..10657
                     /gene="rpsP"
                     /locus_tag="IWK54_001974"
     CDS             10382..10657
                     /gene="rpsP"
                     /locus_tag="IWK54_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9594091.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10669..10920
                     /locus_tag="IWK54_001975"
     CDS             10669..10920
                     /locus_tag="IWK54_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9594092.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11045..11836
                     /locus_tag="IWK54_001976"
     CDS             11045..11836
                     /locus_tag="IWK54_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594093.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12006..12527
                     /gene="rimM"
                     /locus_tag="IWK54_001977"
     CDS             12006..12527
                     /gene="rimM"
                     /locus_tag="IWK54_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9594094.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12517..13278
                     /gene="trmD"
                     /locus_tag="IWK54_001978"
     CDS             12517..13278
                     /gene="trmD"
                     /locus_tag="IWK54_001978"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9594095.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13414..13761
                     /gene="rplS"
                     /locus_tag="IWK54_001979"
     CDS             13414..13761
                     /gene="rplS"
                     /locus_tag="IWK54_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9594096.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13893..15029)
                     /locus_tag="IWK54_001980"
     CDS             complement(13893..15029)
                     /locus_tag="IWK54_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9594097.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(15145..16059)
                     /locus_tag="IWK54_001981"
     CDS             complement(15145..16059)
                     /locus_tag="IWK54_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP9594098.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(16150..16578)
                     /locus_tag="IWK54_001982"
     CDS             complement(16150..16578)
                     /locus_tag="IWK54_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9594099.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(16583..16957)
                     /locus_tag="IWK54_001983"
     CDS             complement(16583..16957)
                     /locus_tag="IWK54_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9594100.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            17243..17428
                     /locus_tag="IWK54_001984"
     CDS             17243..17428
                     /locus_tag="IWK54_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9594101.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            17440..17688
                     /locus_tag="IWK54_001985"
     CDS             17440..17688
                     /locus_tag="IWK54_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594102.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            17719..17898
                     /locus_tag="IWK54_001986"
     CDS             17719..17898
                     /locus_tag="IWK54_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594103.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17941..18411)
                     /locus_tag="IWK54_001987"
     CDS             complement(17941..18411)
                     /locus_tag="IWK54_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594104.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            18498..19196
                     /locus_tag="IWK54_001988"
     CDS             18498..19196
                     /locus_tag="IWK54_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9594105.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            19374..19715
                     /locus_tag="IWK54_001989"
     CDS             19374..19715
                     /locus_tag="IWK54_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594106.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            19708..>19997
                     /locus_tag="IWK54_001990"
     CDS             19708..>19997
                     /locus_tag="IWK54_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9594107.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         6561 a         3509 c         3933 g         5994 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6421 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6481 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6541 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6601 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6661 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6721 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6781 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6841 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6901 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6961 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     7021 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     7081 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7141 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7201 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7261 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7321 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7381 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7441 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7501 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7561 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7621 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7681 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7741 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7801 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7861 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7921 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7981 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     8041 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     8101 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8161 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8221 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8281 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8341 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8401 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8461 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8521 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8581 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8641 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8701 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8761 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8821 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8881 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8941 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     9001 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     9061 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9121 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9181 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9241 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9301 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9361 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9421 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9481 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9541 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9601 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9661 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9721 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9781 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9841 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9901 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9961 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    10021 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    10081 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10141 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10201 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10261 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10321 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10381 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10441 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10501 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10561 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10621 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10681 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10741 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10801 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10861 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10921 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10981 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    11041 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    11101 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11161 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11221 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11281 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11341 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11401 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11461 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11521 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11581 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11641 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11701 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11761 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11821 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11881 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11941 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    12001 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    12061 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12121 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12181 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12241 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12301 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12361 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12421 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12481 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12541 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12601 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12661 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12721 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12781 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12841 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12901 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12961 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    13021 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    13081 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13141 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13201 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13261 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13321 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13381 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13441 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13501 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13561 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13621 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13681 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13741 atcaaagaaa tccgtcgtta atcatcgtta tcagactgag aaagaaccct tggtattcaa
    13801 gggttctttt tttgttctct gtttttttat attaacgatt tctatttggg acatttttga
    13861 ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt tttctatatc ttctttcaat
    13921 ttttctttta ctccctttgt cacatgcaaa taaatctttt cagtgatatc actattttcg
    13981 tgcccaacgc gatcctgaat agcatacaga ggagttccta gttccgctaa ttttgaaata
    14041 tgggtatgtc taaaaatatg tgaactaagt tttttatcaa tcttcatgtc agctttatga
    14101 tttctcaaat aggtgttaat tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat
    14161 agaaattgac cattcggatt taattctaga agctcattgt aaatagctat cgcttttttt
    14221 ggtaaatcaa tttctcgcat tccagcagca gtttttgtag aatcagattt tttcatatca
    14281 gctattgaac gttctctata catcatcgtc ccatttataa ctactgatgc attattattg
    14341 gtaatgtgta catcgtcttt acttaatgcg attgcttctc caggcctcat tccagtcaaa
    14401 tatagccact ggaaaaggag agaatatctt ttattgtgtg aagttgtgaa atttactagt
    14461 cttttatatt catcatcttc taaaaactta tctttaattt ttatcgtttt tgactctcgt
    14521 ttataatcaa taactacttc gttgatagga tttttttcta catagccttt tttcatagcg
    14581 tatgaaaaaa gaagattcaa tttggattta attacactga cgtacttatt tgacaagtca
    14641 tctttatata tcatatcttc aaaaatatta tttaaatcta ctgtattaat accagaaacg
    14701 atgtaagtct caggtatctt tttctttata gtgtttaaga tattattcgt aggataatat
    14761 gtagactcct taacttgtct tttgtaaata actaaccatt cttcgacaag ctcatgaaat
    14821 gttagatcag gcttctgaag tgttttttcg tttaatttta tatcaatttt tttatttaac
    14881 tctagcaatg ccactttctg agtttctcga gatttatttt tataagtaat gctcactttt
    14941 tttctttttc tagtttttgg atcaatatat cgctcattat atttatatac tttttttccg
    15001 tttttatcag ttttggtttc aatccacatt gttttcatct cctatttgtt gctataatag
    15061 gaatggataa gtaagccaat tatagcaggt ttatttttca tcacgtccac aaactttggc
    15121 gagggagggg gcgtgttttt ttgtttagta gctacctttc catgaagtat ctgcgaagaa
    15181 cacttcaaat gttgttccat catttttcac ttcaaaatat gctgttcctt gtgcggattt
    15241 acccggttgg atggtttccg aaaagtaatc tctactagct aaatcagatt taatatctga
    15301 tgaatcataa aattctaaat aacttgaact tgcatcaaaa ggtgtagtcc ctgtgttttc
    15361 tacaagaaaa tctacctttg cgtaaaatag gccttctggt ttatagaaat catcaccgta
    15421 atcttttgat acagactgga cagttacgtt aatagagtta ccattttcag aagtaaaagg
    15481 aactgcttct cctatactat gtgcaactga tgccgtctgg ggttctgaag acgaagttgt
    15541 agttgtagtt gtagtagagc ttgaatcttt ggattttcca tctttatcta agaaagttcg
    15601 ttgattataa tatacaacct gttcagtttt tggactaacc caaattaaca ttgtttcatc
    15661 atcagaatca gttgaataag tatattggag catctctaat ttttttatat cttttgcact
    15721 atttccatgt tcaattaatt cagagtgctc tttataataa tctccttgtg catcctttga
    15781 aatagataca attaattcat cccgtgtgat agcattgtct aattctttgt aaacaaaatc
    15841 atttgtccat ttacgtaacg gtgaaccaag tgtctgataa acttgagtta aatcatcacc
    15901 aacttttatt ttatgaacgt ctgatgaagt tagttttttt gtttcatatt cagatgttga
    15961 tgaaacagta ttttctttag tgctagcaga attaggacta gtggagcatc cgcttaatac
    16021 caagcctaaa aaaagcaacc caaaaacaat ttttttcatt tttaattcct cacttctatg
    16081 atatgatttt tttgtaggat cttagaaata aggtttttag tccgtgttgc agcacggact
    16141 ttttttacat tatataagaa agcgataagc gctttctgga agtccgtaaa gattctttaa
    16201 ttcctcaatt cgtttaggat attgatcatt gtcttctttg tatagagaaa caatgagatt
    16261 agcagcaaag caattagctt cgctttcaga tttgcttcta gatgttcttg ttgatacata
    16321 gtaactggat aagccacgat gaaaaatagc gtgacctaat tcgtgagcgc aaatgtagaa
    16381 tctttcctca gagtctctca gttcatcatt taagaagatt attgcacgac ctctaatttc
    16441 ttgaaactgt cctttgggat tctcgataaa aggaacgtat tgaattttaa tgcccatctt
    16501 ttcacaaata taaaaaggat tagcggactg gtatttccgc ttcaactcct cgactaaatt
    16561 aatcgtatcc atctccataa gctcacatct ttttgccttt ttctttgtct tctttcacaa
    16621 tatcccagaa ggttccaata aggatatctt ttacgcgttg gacctgttca ggcgtcagtg
    16681 tttctccgcc ataagacata ttgacatttg aatctagtaa tttatcaagt tcaactactt
    16741 catcttttgt tgcccagtca ggaacttggt ttctaccgag taaataatca gtagttacat
    16801 taaaataatc tgctacaagt tgtagtcttt tagtactagg agttctattt ttccattgat
    16861 aaatcgtgtt tttaggtata tttaattctt cttccaattg agttatactt atgcctcttt
    16921 tatgagctaa ttcctttatt ctatctagta aattcatttt cgcacctcac aagctacgaa
    16981 aacactaata aaaaaattag ttaaaggtgt tgacaactaa taaaagtgtt agtatactgt
    17041 tttcgtaagc taaaatatta gctaaaaaga cttgataact aataaacact tcacggtcgg
    17101 caaactctga aatgtaaatt actaggcatt tctgtgtctt atttagctat gtctatatac
    17161 taataaaaat attagttatt gtcaacgaat attagctaat tttttagctt acagattatt
    17221 tttttagaaa ggagctattt ttatgtctga gaatttagac ttaaaaattc gagcggagat
    17281 gagaaaaaga agaatgactt tcaaagaact agctgcgctt atcggtattt caggagctta
    17341 tttatcagat attctaaacg gcaatcgtga tggaaagaaa gcacaacagc atatcgaaac
    17401 agtgaaaaaa atattggaca tccgataggg gggtaggcga tgacaaaact aaaaaaacaa
    17461 gattttgtaa aaaaatacaa ttattctcca tctacttatc aacgtcgaat gtcggaacta
    17521 aaaaatacag caattttctc agcggcgtat gaacgggtca caggacaaga agtttggatc
    17581 aatacagaat tatacgataa atttttgtct ttcaaatcct ataacaggtt acgcacaaga
    17641 aaggtaacac ctaaagaatt tatcgagaag catttagttg atttataaaa aacagaacat
    17701 tttgagaggt gaaggttaat gggtaaattc aacagagcat tggtattcag cgcaccgcta
    17761 atcatctacg ctttaggact ttggggaagc aggcaagcgt tgataggaac gattgtttac
    17821 atggtttgga tttttatggg gcttgatgaa gctgaagctg agtacagagc gaaaaagcca
    17881 accgaggggg ctgactaaaa atgaatagaa aagagaaact agaatgtata ttactattac
    17941 tcagtttaat tctttcacta atttctctat tgggaagttt gtatttttga tatcaacaat
    18001 tttgtggatt tgggttctat ttagagtaaa tatcattttt gtttggtgat taaggagttt
    18061 attaaaatta attttcccta cttcaaatgc aaggtaacaa gatatagcac ttttaggagg
    18121 aatatttagc ggtacagctt cagagtatat tctattatca tcgactgatc ctcctttagc
    18181 aattaatttc ttaaatggag tagctgacca tctgcggttt aagttagcgg atgaaagttc
    18241 taaattaact aacatagctg gttctgttga atagttagaa atgattactt ttgtataaat
    18301 catatctgca gcgaagtaag aagcattaag ttcaacttct agttgagggc gcattccttt
    18361 tatttttatc cctgttaaaa acgtactcaa tataaaactt attattgaca tccattgaaa
    18421 aattgttaag tgaaaaaaat tcaaaataaa caccaccagt ttttaactaa attataccaa
    18481 aaaggagaga agaaataatg caagaattag taattttgaa aaataaagaa gctgtgacta
    18541 cgagcttaca agtggcagaa agcttcgaga aaaaacatca acatgtttta agagatattg
    18601 atgcactaaa aaaagatgtg tccaattttg gacagatgtt tgtagaaggt aatgaaccag
    18661 attcatatgg cagaaatcga cgagttttct tcattagtag agatggattt ttcttgctgg
    18721 ctatggggtt tacaggaaag aaagctatct acttcaaaca aaaatacatt gaagcattca
    18781 acgaaatgga agatgttatt cgcaagaata ctgttcctca aacaattgaa gatatgatga
    18841 tctatcaatt agaagaaatg aaagatgtta aaaaagatgt ttccatgctt aaagatacta
    18901 tgcgaattag cggacaacaa gagtttgaaa ttaagcaaaa aggaaatatg aaagttatgg
    18961 gagttctagg gggaaaagaa agccgagctt atgaagaaat cagcaaaaaa gtattctcaa
    19021 aattttggtc tgaatttaaa cgtacctttt caatcccaag atatggcgag ttacctcgta
    19081 agagattcga tgatgctgtt tcatttattg aaatgtggtt gccagaaact gcgatccgta
    19141 tggaaattga tcaactgaac agacaacaga gactttttgg tgatgacaat gaatagagct
    19201 gaagcgctaa gaatagggac ggcaattgct aattgctggt ggaaatacta taaaccaatc
    19261 atcctaagcc aacaacatat tgacaagcaa aaagcatggc aacaaataaa aaagtgactc
    19321 cgccggcaag caaagagtca caaagaaaac acatcataag gagattttag catatggaaa
    19381 aagaactttc cactctagat caatatttga ttgatcctga ttggggcaag ccgaaaattg
    19441 aggaaacaag tggtcgaaaa atcagacgaa atcttttgac gaatgaagaa ctagcttggg
    19501 atcaagatga tttaggcaac catgtaacta tttgggatca tgtttatctt atccatctat
    19561 cgaagcattc gaataaacct gaatatattt acgtcatcga agatggcttg attgatgcgc
    19621 tagaagagta cgacagagat aacttgattg atatctctta ttacggacca ggtaagaaat
    19681 acattgctga aatggaggca gaatttgatg agtgaaatca aagggacaac gaactttgaa
    19741 aaacttttta gtcgtaagtt aaataaaatt ctcaagaaaa aaggaaattt tgattattta
    19801 tcttgggctc acgcgtggga gattatgaaa aagaatgatc cacaggcaac ggtaactatt
    19861 aatgagtaca aacactacag ggttgtttct ggaactcatc aagactttct tgttgaggaa
    19921 tataaacctt ttcttatgga tgaaactggg acttatgtat ctgtctcagt aacggttaaa
    19981 ggacacacgg aaaccga
//
LOCUS       DACDTH010000052        19821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDTH010000052 DACDTH010000000
VERSION     DACDTH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.052"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWK54_001991"
     CDS             6..305
                     /locus_tag="IWK54_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9594108.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWK54_001992"
     CDS             378..1739
                     /locus_tag="IWK54_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9594109.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWK54_001993"
     CDS             1761..2021
                     /locus_tag="IWK54_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594110.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWK54_001994"
     CDS             2035..2787
                     /locus_tag="IWK54_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9594111.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWK54_001995"
     CDS             complement(2881..3486)
                     /locus_tag="IWK54_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9594112.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWK54_001996"
     CDS             complement(3539..4513)
                     /locus_tag="IWK54_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9594113.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWK54_001997"
     CDS             complement(4891..5670)
                     /locus_tag="IWK54_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9594114.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWK54_001998"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWK54_001998"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9594115.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWK54_001999"
     CDS             7161..8558
                     /locus_tag="IWK54_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9594116.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWK54_002000"
     CDS             8639..9079
                     /locus_tag="IWK54_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9594117.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWK54_002001"
     CDS             9227..9553
                     /locus_tag="IWK54_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9594118.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWK54_002002"
     CDS             9554..10303
                     /locus_tag="IWK54_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594119.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWK54_002003"
     CDS             complement(10383..10838)
                     /locus_tag="IWK54_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9594120.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWK54_002004"
     CDS             complement(11265..11540)
                     /locus_tag="IWK54_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594121.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWK54_002005"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWK54_002005"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9594122.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IWK54_002006"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IWK54_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9594123.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="IWK54_002007"
     CDS             14770..17436
                     /locus_tag="IWK54_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9594124.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="IWK54_002008"
     CDS             17800..18552
                     /locus_tag="IWK54_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9594125.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="IWK54_002009"
     CDS             complement(18574..19728)
                     /locus_tag="IWK54_002009"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9594126.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6330 a         3472 c         4060 g         5959 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagttt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DACDTH010000053        19475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDTH010000053 DACDTH010000000
VERSION     DACDTH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.053"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(21..995)
                     /locus_tag="IWK54_002010"
     CDS             complement(21..995)
                     /locus_tag="IWK54_002010"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9594127.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="IWK54_002011"
     CDS             complement(1075..1950)
                     /locus_tag="IWK54_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9594128.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="IWK54_002012"
     CDS             2080..3987
                     /locus_tag="IWK54_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9594129.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="IWK54_002013"
     CDS             complement(4528..7806)
                     /locus_tag="IWK54_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9594130.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8077..8622)
                     /locus_tag="IWK54_002014"
     CDS             complement(8077..8622)
                     /locus_tag="IWK54_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9594131.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8634..9326)
                     /locus_tag="IWK54_002015"
     CDS             complement(8634..9326)
                     /locus_tag="IWK54_002015"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9594132.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9345..9629)
                     /locus_tag="IWK54_002016"
     CDS             complement(9345..9629)
                     /locus_tag="IWK54_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594133.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9642..10205)
                     /locus_tag="IWK54_002017"
     CDS             complement(9642..10205)
                     /locus_tag="IWK54_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9594134.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10433..11095
                     /locus_tag="IWK54_002018"
     CDS             10433..11095
                     /locus_tag="IWK54_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9594135.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11219..12415
                     /locus_tag="IWK54_002019"
     CDS             11219..12415
                     /locus_tag="IWK54_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9594136.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12516..13892)
                     /locus_tag="IWK54_002020"
     CDS             complement(12516..13892)
                     /locus_tag="IWK54_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9594137.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13929..14795)
                     /locus_tag="IWK54_002021"
                     /pseudo
     CDS             complement(13929..14795)
                     /locus_tag="IWK54_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(14884..15909)
                     /locus_tag="IWK54_002022"
     CDS             complement(14884..15909)
                     /locus_tag="IWK54_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9594138.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15927..16601)
                     /locus_tag="IWK54_002023"
     CDS             complement(15927..16601)
                     /locus_tag="IWK54_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9594139.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16785..17678)
                     /locus_tag="IWK54_002024"
     CDS             complement(16785..17678)
                     /locus_tag="IWK54_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9594140.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17752..19449)
                     /locus_tag="IWK54_002025"
     CDS             complement(17752..19449)
                     /locus_tag="IWK54_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9594141.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4190 c         3444 g         6157 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa
     7981 aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat
     8041 tttgtgcttt ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt
     8101 ggactcaaaa tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc
     8161 tttgaaaata tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca
     8221 gatatctgta catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat
     8281 gtctagatct gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc
     8341 ctcttgctca tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt
     8401 tcctatcaca ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc
     8461 atgcgcatca atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc
     8521 agtcagcttt ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc
     8581 aacaaaatca tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg
     8641 agatgtttga caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat
     8701 tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa
     8761 aatgggatat tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct
     8821 aatgcttcag ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta
     8881 tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc
     8941 atttcactgc gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct
     9001 ggtttgtagc caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg
     9061 atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg
     9121 ttcacgccgt attgttggat caacagactt gtggtaatag atgctaagac ttttcctata
     9181 ccggaacgta cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct
     9241 gcgcgttccc atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc
     9301 attgcgccga taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt
     9361 gctaaaaaaa ccacacataa cattaggata acaataataa gagatttcat caaaaaagag
     9421 ttccatttgg tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt
     9481 ttgctttctt tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct
     9541 gcttttcgct gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg
     9601 cttcgcgtaa taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat
     9661 agttcccagt attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc
     9721 gcttgttgcg cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt
     9781 gctaaaggat tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt
     9841 gcaaaacctg gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca
     9901 gtttcttctt ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg
     9961 atttttccag ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca
    10021 agaagcagac gatcttgttc gtcaaaagca atgatcccga caccaccagg atgaaatacc
    10081 agttctctct tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata
    10141 atttttccgc tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc
    10201 agcatatctt ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt
    10261 tctcaaaaac tccttattag tttaccatag ataaacctta gattttctct aaatatagaa
    10321 aatccttctt tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc
    10381 gaataacgaa aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa
    10441 acgaaattgg ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag
    10501 tttttctttt gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt
    10561 tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga
    10621 aactcactat gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat
    10681 gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact
    10741 taaagcaatc gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt
    10801 ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa
    10861 tctagttgat ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca
    10921 gacctatgaa aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc
    10981 acaagattat caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt
    11041 tgcgaaacag agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt
    11101 ttgttcataa aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt
    11161 ctaaagtaga gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat
    11221 ggatcataaa ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt
    11281 attgaacaac ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat
    11341 tgatcagctc caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg
    11401 ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat
    11461 cagctacgga tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca
    11521 tgtgcaagca caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct
    11581 ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt
    11641 cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca
    11701 aattgacaag atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct
    11761 gatgttagaa cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat
    11821 cgctgctgga gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa
    11881 aagagcagaa gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt
    11941 cctagatcgt ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca
    12001 gcaagcacca caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca
    12061 agcatctatc gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt
    12121 gcttcgtcaa aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt
    12181 attgaagaaa aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga
    12241 acgtggaatc gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat
    12301 ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga
    12361 gcttggacac atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac
    12421 ctttcgcttg ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt
    12481 ttttcttatc tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt
    12541 ccatagccat agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc
    12601 catgtcacag tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc
    12661 aatagcggaa cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc
    12721 aacttcagta agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt
    12781 tccgctaata cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca
    12841 taagtcgctt ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt
    12901 aagccatcct tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt
    12961 accgtcagta gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct
    13021 acatacctca ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt
    13081 cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg
    13141 attcctcttg gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca
    13201 tcaaatccca agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca
    13261 aaaggcatga atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac
    13321 aacgcgatga taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg
    13381 gcttcaagcg acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg
    13441 tatgtaccat tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa
    13501 cttttcaaaa aaccttgcat atagccagac catccggaag caacagagga aaccgctagt
    13561 aaaaattccc cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca
    13621 tacagatagg catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt
    13681 agtccagaca aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca
    13741 gcattttgat tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca
    13801 attccaagca tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg
    13861 agattgatct ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa
    13921 aacagcgatt aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg
    13981 caaccaacaa taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc
    14041 ccacctaaag tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca
    14101 agtgatttga ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc
    14161 atgtttttcc acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct
    14221 aagatcattc cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg
    14281 acgatgatgg tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta
    14341 ccaaaatcaa gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc
    14401 aaatacgcac cgcttacaag aagagctaaa gcaataatac cctatacata atctttcgtt
    14461 tgtgtccatt catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca
    14521 gtagacaatg gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt
    14581 tgtccaacac tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt
    14641 acaggactaa caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct
    14701 agtgtttgtt gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat
    14761 gccaacatag ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg
    14821 ataagaaact tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat
    14881 tttctaacgt ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc
    14941 ttttccttca gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc
    15001 taaataaagt cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa
    15061 tcccttatca aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac
    15121 tgaaaggctg actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg
    15181 cgtatatttg atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc
    15241 ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata
    15301 atttgcctga ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag
    15361 gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc
    15421 gatttttgat aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat
    15481 cgaacgaact agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc
    15541 aatataatct ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc
    15601 acggatcagc tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact
    15661 caaataattt tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct
    15721 cttgctcaag taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa
    15781 ataagcaatt gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt
    15841 caatgcaata aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa
    15901 aaaattcatc ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt
    15961 tcgatatatc cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact
    16021 gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc
    16081 cgacttaaga tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg
    16141 ctcaaatcaa tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg
    16201 ccattgtggc tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat
    16261 gcattgattt ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc
    16321 cccatgttca tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc
    16381 ggtactttcg atacttcacg tattttttga caccaataat agccatccaa aacgggaaga
    16441 ttaatatcta gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg
    16501 aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt
    16561 cgaatgatct cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt
    16621 cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat
    16681 gtcattttag caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt
    16741 ccaagcagct taaatttatt atgttattct taataattgg tctattatct gttatttttc
    16801 tttcgttgtt tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact
    16861 agcaatatac ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct
    16921 atctttgaag agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga
    16981 gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt
    17041 gctaatgtat ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc
    17101 atccgaccat acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta
    17161 aagagttttt tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct
    17221 ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc
    17281 gtttcatcaa ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata
    17341 tgttgttcgt aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga
    17401 tctttgactt gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt
    17461 tcatgcactg ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga
    17521 taattctcga tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat
    17581 gtaaaaactg cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg
    17641 atttgggttt gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa
    17701 aaaactgcag caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc
    17761 agacttcatt ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat
    17821 tagtaagaat gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt
    17881 cggtactctt tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct
    17941 tccataagag atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc
    18001 tggcgccatg gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta
    18061 tccatttcca tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc
    18121 tgctcctgat tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga
    18181 acggtcttca ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc
    18241 atatttttca aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc
    18301 ataatcaaat tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc
    18361 cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc
    18421 atctgctaat ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt
    18481 cacgacaaac ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt
    18541 ctcgccggct cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc
    18601 tgtgattttg taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata
    18661 aggaataggt gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga
    18721 aatcgtttct tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc
    18781 tacattcatc tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt
    18841 attgtatgtg tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat
    18901 cactggcatc ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt
    18961 ttctaagaaa agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt
    19021 agagaatacc gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata
    19081 ttttaaaaac caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt
    19141 aagaccgtct tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc
    19201 cgttacgtca aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga
    19261 aaccaatccg ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt
    19321 aatcgtcact tcacttggtt tttgtgcgac ttcttcggct ttgactagtt ttccgttccc
    19381 gaaaatccct attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt
    19441 tttattcatt tttttctccc cgttcttttt ttatt
//
LOCUS       DACDTH010000054        19207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDTH010000054 DACDTH010000000
VERSION     DACDTH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.054"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(28..327)
                     /locus_tag="IWK54_002026"
     CDS             complement(28..327)
                     /locus_tag="IWK54_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594142.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(333..563)
                     /locus_tag="IWK54_002027"
     CDS             complement(333..563)
                     /locus_tag="IWK54_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594143.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(999..1565)
                     /locus_tag="IWK54_002028"
     CDS             complement(999..1565)
                     /locus_tag="IWK54_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9594144.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1565..2521)
                     /locus_tag="IWK54_002029"
     CDS             complement(1565..2521)
                     /locus_tag="IWK54_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9594145.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2521..3177)
                     /locus_tag="IWK54_002030"
     CDS             complement(2521..3177)
                     /locus_tag="IWK54_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9594146.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3547..5565
                     /locus_tag="IWK54_002031"
     CDS             3547..5565
                     /locus_tag="IWK54_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9594147.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5992..8406
                     /locus_tag="IWK54_002032"
     CDS             5992..8406
                     /locus_tag="IWK54_002032"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9594148.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8754..10307
                     /locus_tag="IWK54_002033"
     CDS             8754..10307
                     /locus_tag="IWK54_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9594149.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10688..12472)
                     /locus_tag="IWK54_002034"
     CDS             complement(10688..12472)
                     /locus_tag="IWK54_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9594150.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12487..14208)
                     /locus_tag="IWK54_002035"
     CDS             complement(12487..14208)
                     /locus_tag="IWK54_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9594151.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            14308..15069
                     /locus_tag="IWK54_002036"
     CDS             14308..15069
                     /locus_tag="IWK54_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9594152.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            16036..16304
                     /locus_tag="IWK54_002037"
                     /pseudo
     CDS             16036..16304
                     /locus_tag="IWK54_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16976..>19207
                     /locus_tag="IWK54_002038"
     CDS             16976..>19207
                     /locus_tag="IWK54_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594153.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6309 a         3406 c         3830 g         5662 t
ORIGIN      
        1 aagcgagttt gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt
       61 atactttata tagtcataac gccgatgatc gctacgtgcg tccggcacat cagtcacgat
      121 atcaaacaaa aaatatacgt ctttcttcat tctagttttc gcagcaggga ttttaaagta
      181 gttcttatta gaatagtaga gattaattaa taagctatct tcgattgcta aaaagaaaac
      241 ttctgaatcc cacaccttat aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa
      301 ccataattgc gtctcattaa aaagcataac cattactccg atctattttt aaacttagtc
      361 tctacctcta atatatatcg agtttttatt ttgccttcag agaatactgt ttcttttttt
      421 gcagttacag gttgtttatt ttcggaaaaa gctaatatag ctaaaattga aacatccatc
      481 tggaatttat cttttttact gctttgatca taaaagtctg catattcatc actgatattt
      541 tttctaataa attcttccat cataaaaatc acctcaaaac gattatacga acttacgttc
      601 ttgttgtaaa gcgatatttg aggggcaaaa aaggggcata atttgactga tattaagtta
      661 gctattgata tatgaatgac atacaaaaat ataaaatctt ttaaaaatgc ttatatatca
      721 atgtttttgc cattctttta tatatctgtt atatgtcaaa aaaatgtgtt caatttcaat
      781 ttgtatttga ataaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca
      841 atcaaaaaca gcttctcatg tttttgattt ggttggactt attaccgcag atataagctt
      901 ttgagcaccg tgaataaaga ggttatgaaa tcaacgctct aacctgagta ttagagaaaa
      961 aaacgcaaaa catatcattt atgttttgcg ttttttattt atcttttttt ctcaatacaa
     1021 tataaaatag gcggctggtt cttttggttg ataaaccgat aattcagcac attatatgtt
     1081 tcctgtggca gtttggaaca aaaagaatca accatatcca gttctttttc tccaccttca
     1141 tgaccatagt agacgacaag gatcaatcgt ccgcgaggca caagtcgttt caatatttct
     1201 tccatagctg tgcgtgtagt ctctggcata gtgatgattg ctttatcact ttttgggaga
     1261 taccctagat tgaaaatccc tgctttgaga ttttgttgtt cagctatcac ttctcctaac
     1321 gtttcgtggc cttgtagaaa caatgagaca cgttcttcta actgacgctg ttctagcctt
     1381 tcacgagtag catcgattgc ttgttgctgg atatcaaaac tatagacatg accagttttt
     1441 ccgatgtgtt ctgctagaaa aaccgtatcg tggccattgc ccatcgttgc atctacgacg
     1501 tgatcccctg gttgcaagat ttcttgcagt aatgtatggc tgaaatgcaa agcagtttgc
     1561 aacatcagac gctcaccttt ttccgtacat catattttcc ttggaagcta tctctgcgaa
     1621 gaagttcttc atcaattgca ttcaagactt cccatttttt caagctccac attggtccaa
     1681 tcaaggaatc ccacggcgca tcccctgtca aacgatggat gatgatctct tttggtatca
     1741 tttccagctg gtcgcaaatg acattgacat agtcaggacg gctcattaat tgaaggcgac
     1801 cttcatgata gtcccgcaac attctcgttt tgcgcatcag atgcatcaaa tggagcttga
     1861 ttccttggat atctgaatct aaaatggtgc gtctgacatt ttctttcatc atttctaaac
     1921 tttcgcctgg taatccgttg atcaaatgcg tacaaacacg gatattatgt ttgcgtaatt
     1981 tagccacgcc atctaaataa gtttgataat catgtgctcg gttgatcagt tttgaagttt
     2041 cttcaaaagt tgtttgcaat ccaagttcta cccataaata catccgctcg ttcagttcag
     2101 caaggtaatc aactacttca tctggcagac aatccggtcg tgtaccgact gataatccta
     2161 cgacacctgg taaattgact acttgctcaa atcgttcttt gattatttca actggcgcat
     2221 gggtgtttgt aaaattttgg aaataaacga tgtattgttg gacatgaggc catttttgat
     2281 gcatcatgtc gatttctttc tggaattgga ttggaagagg gtcttcaggt gcaacgatca
     2341 tgtctcccga accagataca ctgcaaaaag tacaaccgcc atgggcaaca gtcccatctc
     2401 gattcggaca gtcaaatcct ccatcgatcg gcaccttgaa aattttcccg ccaaactcat
     2461 ttcgtaaagc gtaattccaa gaatgatatc ttttatttcc ttctgaataa ggaaaagtca
     2521 ttaaaagtcc ttctttctgt aagagttatc tatttgtttt cttttttctc tatacactgg
     2581 ataagtcagg cacagccaag aaagactgag taagtaaccg ccgatgatat cagtaggaaa
     2641 atgaacgccc agataaatgc ggcttatccc gacacttact ataataaaca tcatcagcaa
     2701 ttgaagcaat gtgcgtaagg ttcttttttg aacaaaagct ggaagcagaa atagtaaaga
     2761 accgtaaaga atcatactgc ctgctgcatg tccactaggg aaactgtaac ttgtttcaac
     2821 tactaagtgc gccaatgaag gacgctctcg catgaaaaat agtttgatca ccgggttgat
     2881 gattccagca attacaataa atccagaaac gaaccaaaat gtttctgccc tttttttctt
     2941 ataccacatc cataaagccg cacaagcaaa taaaatgatg acggtaagag gattaccgaa
     3001 tttagtcata aagagttggt agctgttcag atcaggataa gaagctctga caatgcctgt
     3061 aaaccactga tcgaaaccag aaagccacga cagatggaac tttacgatcc agcccaaaat
     3121 gaaaaacagg acaagaaaga agctaccagc cacctgaaat gatgttttat atttcataca
     3181 attcctcctt tgaagcaatg acttattata gcatttttgg tccagcgaaa aaaacggtaa
     3241 agagataaaa ccaatgtatg attcctcttt gaataaccaa tattcttata aaaaaaataa
     3301 ctgtaatcgg aaagacattt tttggtaatg caaatgaaat gttagggaaa taaaatcagt
     3361 gtttttcgaa atccttttaa acaagaacgt agacgtagga agaacttgaa aatgaacaga
     3421 agttgttcat tacggtaaat tacaatatga ttacaaaaaa caacaaagct cttaaataac
     3481 ttttaataaa tattaagaga agttataatt attaataata gataaattga agttggagga
     3541 tagaagatgg aaaacattgc aagaaaagaa cggagacgtt tagaacagac caaacgtttt
     3601 cgaaaagcaa aacgaggggc agcaatcgtg ggaactgcga tggtaggctg ttctgtcgct
     3661 gccccattag tacagccggt tcctgttcaa gcggatgaaa cacctgttca gttcggcgca
     3721 agaatcaata cagcggcatt tatcacagaa attgctactt atgctcaacc aatcgctcaa
     3781 gccaacgatc tatatgcatc agtgatgatc gcacaagcag ttgtagaaag cggttggggc
     3841 agtagtgcgt tatctcaagc accttattat aatctttttg ggataaaagg cagctatcaa
     3901 ggacaaaccg tctacatgga tacgcttgaa tttttaaata atcaatgggt aaataaaaag
     3961 gagcctttcc gacaatatcc ttcttttgct gaatctttca atgacaatgc ctatgtactt
     4021 agaaatactt catttggcaa cggttattac tatgccggta cttggaagag caacacgaaa
     4081 tcctatacag atgcgacagc ttgtttgaca gggcgttatg cgactgatcc tggttacgca
     4141 ggcaaattaa ataatatcat cgcaacttac ggattgaccc aatatgatac accagctagc
     4201 ggaaacgcag gcggtggagc gactacaggt ggcaataata ctggaagtgc aggtagtact
     4261 ggcggttcaa gcaattctgg taatacaaac tcaggttcag caacttcgac cacttatact
     4321 gtccaatctg gtgattctgt ttggggaatc tcaaataaat tcggtatcac aatggatcaa
     4381 ttgatccaat ggaacaacat ccaaaacaat ttcatttatc caggacaaaa actgacaatc
     4441 aaaggtagcc aggaaaatgg ctcttcgacg aataattccg gtaacaatac caattcttca
     4501 ggaaatgctg gtacatcaaa cggaggacaa acaactggtg cgaaatacac tgttcaatct
     4561 ggtgattctg tttggaagat ctcaaatgat cacggaatta cgatggctca attgattgaa
     4621 tggaacaata tcaagaacaa tttcgtttat ccaggacaac agctaatcgt atcaaatggc
     4681 ggttcttcta atgctggagc aaatactggc tcaaatactg ggaatacaag tggctctagt
     4741 tcgtccagct caagttcttc taatacagct tcaggaacga aatatacagt taaagccgga
     4801 gactctgttt ggagcgtctc aaataaatac ggaattacga tggctcagct aatccaatgg
     4861 aacaatatcc aaaataactt tatctaccca ggacaacaac tgactgtttc aaacagtgga
     4921 tcaacaaaca gcacgtcagg taatacaggt tcgtcaacca atacaaataa cagcagttca
     4981 gttaatactg gcagcagttc gtctcaaggc acttataccg tgaaagcagg tgaatctgtt
     5041 tggagcgtgg caaataaaaa cggcatcacg atggatcagt taatcgaatg gaataatatc
     5101 aaaaacaact tcatttatcc aggacagcaa ttgatcgtta aaggtggaac ttcggcaaat
     5161 acaaatacag gttcaactac ttctgcagcg aaaccaaata caccgaatac gtctgctacg
     5221 acaagtacat catccactgg taatacgatg tataccgtga aagcgggcga gtcagtttgg
     5281 agcgtagcga acaaacatca tatcacgatg gatcagttaa tcgaatggaa taatatcaaa
     5341 aataatttca tctatccagg acagcaagtg attgtgaaga aaggttcatc tcaatcaaca
     5401 aaccaacaat cagttactgg ttcgaaaacc tatactgtga aatctggaga atctgtttgg
     5461 agtgttgctg acagtcatgg aattacaatg gctcaactaa tcgaatggaa caatattaag
     5521 aacaacttta tttatccagg tcaaacgttg attgtagcta aataaaatag ctgttcgaag
     5581 aaataacgat tgaattccac aaaaatattt gttactataa ggctacttaa atatgacaac
     5641 acttgaaaag gaagtagtaa aaagactgaa gtttcagaga gcgcatggct ggtgagaatg
     5701 tgcacggatg cttttgaact cgcctttgag ttgcccttgc tgaatacccg agtaagcaag
     5761 cggcggtgac ggccgttaca acgttcgagg ccttttcact aaggtaaaat taggtggtac
     5821 cgcgtagatt acgtcctata aaaagagcaa tctttttata ggattttttt atttcttttg
     5881 tgaagtttga gaaaacaaag caaaaggaaa taaaaataaa cagattctgc cggttttttc
     5941 gttatattta taatagaaac aagtgttttc aatagaacag gaggacagca aatgagttac
     6001 aatcataaag aaattgagaa aaaatggcaa aaatactggg caaaaacgaa tactttcaac
     6061 acccatgatg atcctgaaaa gccaaaattt tacgcactgg atatgtttcc ctatccatcc
     6121 ggtcaagggc tacatgtagg gcatcctgaa ggttatacgg caacggatat cctttcacgt
     6181 gtgaaaagaa gtcaaggata caacgtgctg catccaatgg ggtgggatgc attcggtctg
     6241 cctgctgaac aatatgcgct agatacagga aacgatccag ctgagtttac ccaaaaaaat
     6301 atcgaaacat tccgacgcca aatcaattct ttaggattta gttatgactg gaatcgcgaa
     6361 gtgaatacaa ctgatccgga gtattataaa tggacacaat ggatctttac gaagctttat
     6421 gaaaaaggtc tagcttatga agcagaagta gcggtaaact gggtacctga attaggaaca
     6481 gttatttcaa atgaagaagt gatcgatggg aaaagtgaac gtggtggcta tgatgtagtc
     6541 agaaaaccaa tgcgccaatg gatgctgaaa atcactgctt atgctgatcg cttattggat
     6601 gatctagaat tagttgattg gccagaaagc attaaagaaa tgcaaagaaa ttggatcggt
     6661 cgttcagtag gagcaaatat tgaatttaaa gtggctggta cggataaatc ctataccgta
     6721 tttacgacac gaccagatac tctattcggt gcaacttact cggttcttgc acctgaacta
     6781 gatcttgtta gagaaattac gacccctgaa caaaaagctg ctgtagaagc ttatatcgaa
     6841 gagacagcta aaaaatcaga tctaaagaga acggatcttg caaaagaaaa aacaggagta
     6901 tttactggtg cttatgcaat caatccagtt aatggcaagg aaatcccaat ctggatcgcc
     6961 gattatgtat tatcttcata cggcacagga gccatcatgg ctgttccagc acatgatgaa
     7021 cgtgactttg aattcgctca aactttcgga ctagaaattc ttccagttat cgaaggtgga
     7081 gatgttgaaa aagctgctta cacagaagat ggtacgcaca tcaactctga atttctagac
     7141 ggcatgaaca aacaagaagc aatcgataaa atgaatgaat ggctagaaga aaatggcgta
     7201 gggaaaaaag aagtcagcta tcgcttacgt gactggctat tctcccgtca acgttactgg
     7261 ggtgaaccaa tcccaatcat ccattgggag gacggaacag tcacagctgt cccagaagaa
     7321 gagctaccat tacggttgcc taagacaaaa aacatcaagc caagcggtac tggagaatct
     7381 ccattagcaa atatcgagga gtgggtcaat gtcgttgatc ctgtaacagg taaaaaaggc
     7441 cgtcgcgaaa cgaatacaat gccacaatgg gcaggaagct catggtatta cctgcgttat
     7501 atcgaccctc ataacaaaaa agaactagca gattatgaaa agttgaagcg ttggctacca
     7561 gttgacatct acattggtgg agcagaacat gcggtacttc atttactata tgcacgattc
     7621 tggcataaat tcttatacga tatcggtgtt gttcctacaa aagaaccatt ccaaaaacta
     7681 tacaatcaag gaatgatctt aggcgaaaac aacgaaaaaa tgtcaaaatc tcgtggtaat
     7741 gtagtcaatc ctgatgatgt ggtcgaaact tacggtgcag atacactgcg catgtacgaa
     7801 atgttcatgg ggccattaga tgcttcgatt gcttggagcg aaaatggctt agaaggaagc
     7861 cgtaaattct tggatcgtgt atggcgtttg atcgttgatg agaacaataa aatgcgtgac
     7921 cgtatcacta cattgaatga tggcaagctt gataaagtgt atcatcaaac agtgaaaaaa
     7981 gtgacagaag actatgaaaa tcttcatttc aatacagcaa tctcccaatt gatggtcttt
     8041 atcaatgaag cctataaagt ggacgctctg ccttatgaat atatcgaagg atttgttcaa
     8101 ctactcgcac cgattgctcc tcacatgggt gaagaactat gggcaatcct tggaaatgat
     8161 ggtggtattt cttacgcacc atggccaaca tacgatgaag cagcacttgt tgaagacgaa
     8221 gtagaagttg ttttccaagt aaacggtaaa gttcgtgcga aaagcaatgt tccccgagac
     8281 ttaggaaaag atgagctaga aaaagcagca ttagccaatg aaatagtgca agaatatatt
     8341 gaaggcaaaa cagtcagaaa agttattgcg gtaccaaaca agttagtcaa tatcgttgct
     8401 aattaattgg ctctttgtca ataaggactg atgagttgtg caaaatcaaa tctgggccag
     8461 aatgaacttc attctgtctc agattttttg ttatttcaaa ttgattaatt gattgatcaa
     8521 aaaggttttg attttcatgt tctcaaacac gttcaatgtc tttttcttct gaatggtctt
     8581 gtaagagatc tctgtaatca tcaaattttt gtctattaat cttagtattt tttgatgtat
     8641 aaactacctg aaattgagag tacataccaa attcataaat tttttattag tgggactagt
     8701 gattgtatgg attttgacgc ataatgaatg aagtatgaga aaaggagaga caaatggatc
     8761 aagaactatt tattatcgaa gtattatcag ctaaacaaaa aattatcgag aaagcgaaaa
     8821 aaagagcgaa aagaataatg ttcaagcttt taagcttagt atttttctct ggtttgcttt
     8881 ttttcggagc tgtatcgttc ctatatcgcc atatgagtgc agtagtccgt tcagctgaag
     8941 ggatgcaaaa attaaataat ttgtatcact tttcgtgggg agttggcgga ataacaattg
     9001 ttgtgcttat tgtttttctt ctacttattt ggctccgtta ccatcaattg tcgcgtcatt
     9061 tcgatcaatg gcttagtcaa agagcacaac agtttttaca taataaacca acttctcaag
     9121 atgatgacta tctttatttt gagaagactt cgacgagtca agcttttcgg atacaaaaaa
     9181 gtgattgcca gttattgctg agatcacttg atgatttacc tatctactta ggcgaaagca
     9241 aatcaaaatt cggaatgaac acattccata tttttttact agaaaacgaa cccagatggg
     9301 aaaaaagcca gtcttcgcct ggtggatacc tgacaactaa gatgagtaca ttagtcactg
     9361 gttttttcct cattctttta gctggaacaa tctattatgg gagatacact cagccaaaaa
     9421 tgtcttctca tataaatgct gcttctcaca tgacaatgaa ctcaactaac gatacgcata
     9481 tacaaaagac aaatgaaggg ttgttagctg atcctaagca aaaggtaaat ttgacaacac
     9541 aaaagataaa tgatctcaaa cttgatacag agacgcactc tctctatatg acaacagata
     9601 gtggtgctaa ttgggtattt gtgccgattg attcgcaatg gttgcgtttt ggcgaataca
     9661 ccctgacaac ggggctgatt cctgaaggtt attggatgga caacacatat gatatttccg
     9721 ccgatttctc atggtttatc tattcttctg gggatgatgt acgtctgctg acttcaagag
     9781 ataatggtaa aacatggcaa acgtctatta tcacccaatc agttcagccg atacgttatc
     9841 gaaaagcggt attttatggc aacgatggtc tatttatctg tacatcagcc agtgggatgt
     9901 ctgcagaaac tttgtacatt tacactacta tggatggcgg aatcacttgg ataaaaagtg
     9961 gaagtacaat gatcaatcag ccagtacaaa atgtcagctt cgttacaact tctttaggtt
    10021 ttgtttcgac acgtgaaagt ttgttttata caaacaacgg aggacgctca ttcaaagaat
    10081 cagttctgtc gattccggaa aactatcaga caggggggat agatcttttt caatcaccaa
    10141 atgaagttac tcaaataagc gcgaatacgt tagagacaaa attttatttg ttgaaaaaag
    10201 gaccaatcga tgcagggaaa atgtttgcct gcctcttccg ttcaactgat aacggggaaa
    10261 catggcaatt cgttcagcaa ctatcgcaag tagaacaagg agattaaaga agagagattg
    10321 tgagcctgac gttcagcacc gagtcacaag aaacaaattc aaaaaacagc ttctcatatt
    10381 tgttgaatag tgtaactcgt gattcggagt tgacaagagc aacaagaagc gattttgaag
    10441 tatagcttct cgtattttac aagatcgtgt cttattgccg ataggttgtc tcttgaacac
    10501 cgtttattcg taaaaaggga ttgtgacaag tcttttgtca ctacttaccg aacgaaactg
    10561 aaggagctat ccaaaaagta tcattagtat tattctttgt gtttctagtg gcaccactag
    10621 tacatttaaa aaagctatcg atcatggact aatcatacat gatcgatagc tttttagata
    10681 atgtttgtta cgctatctga aattgactat tataaagact ggcataaagc gttggtgatt
    10741 ggagtagttc agaatgtgtt cctttttcga cgatatcgcc attcttcatt acaaggatca
    10801 tgtctgcatt ttcaatcgtt gacaaacggt gtgcaatcac aaaacttgtc ctatcttttg
    10861 tcacagcttc cattgcttgc tgaatcaatg cttctgttcg tgtatctacg ctagaagttg
    10921 cttcatctaa aataacgaca ggaggattcg ctagaatgac tcgagcaatt gtcaataatt
    10981 gttgctgtcc ttgagataac gtgccgtttt cgctagaaat gatcgtatca tatccgtctg
    11041 gcagtgttcg aataaagtga tcacattgtg cgatttcagc cgcttcgata atttgttctc
    11101 ttgtggcatc cattttcccg taggcgatat tatcagctac cgttccttca aacagccagg
    11161 tattttgtaa aaccatccca aaagaacgac gtagttctcc acgtgaaagc ttggtaatat
    11221 caagaccgtc gaattcgatc tttcctttat tcagttcata aaaacgcatc agcagattga
    11281 ctaaagtcgt ttttcctgca ccagttggac caacgatcgc taccgtttgt tttggcttta
    11341 ctgaaaaatc gacttgattc atcaatattt tatctgatgt gtaaccaaac tgtacatttt
    11401 tgaaagctat ttttcctttc ggttgttcta aaagtcttgc atctggaagg tctggaattt
    11461 catctgtttc atctaatatc tcaaatatcc tatcgattgc tgccattgcg gattggatcg
    11521 agttgatcac ataagaagcg gtagaaattg gttcagaaat ctgattgatg tactgaagat
    11581 aagcttgtaa gaagccgatc gtcacaccac ctgatagaac aagcatagca ccgaataccg
    11641 cacttatgat gaaggcgagt tgattaatga aacgaatggc cggatagata gcacagttca
    11701 taaattgagc acgtttaaaa gcgttgtatt gctttttatt tacttcgtgt atagcagctt
    11761 ctgcatgagc ttgctgattg aatgttttga ccactagatt accagctaag atttcttcca
    11821 ttcgattatt taaaacaccc agttcatact gattctgttc agaaatctgt ttgtttttgt
    11881 tcgcaatctt agttgtaaca attgaactgc caacgatcag caataagacg agcaaagtaa
    11941 gcttgctttc gatataaaac agcatcagac cagcagcagc tattgttacg acagaagtaa
    12001 aaaattgatt gatacctgtc aataacactt gagaaagttg attgagaccg gtagttgtcc
    12061 gactaatgat gtcacctact tgatgattgt caaaaaacga catcggtaat gttttgaatt
    12121 tttttgtgat ttcttttcgc accctcaatg tgatcttttc gcttaacgct gccattgtcc
    12181 gttcctgaat aaatgaagtg atgctgctta agactgaaca gccgatcagc aataagacag
    12241 ggaacaacaa tgcttgtttg atatcactga aagaaagctg tccaatacct gtttttctta
    12301 tttctgataa gagatcatcg atcccgattc ccatgatcaa gggcatggct acgactaata
    12361 ggttcccgaa taaactgcat agaaacaatc cgtaaaaata tttatgttca ggtttgatca
    12421 ttttccaaaa acgtatcaaa gcacgcgtgt tcaacgtttt tttcttttcc atcgtatccc
    12481 ttctttctat attccttgtg attttgcaaa ctcctgataa gtttggcaat tagacagtag
    12541 ttcttcatga gtaccttgtc caacgatttt tccttcatct aaaacaatga tgttatctgc
    12601 ttgttggatt gtactgaggc gttgagcaac aatcacgaga gttttctccg acatgtgttt
    12661 atgaagggct gctcttaatg ctgcatctgt tttatagtct aaagcggaga aactatcatc
    12721 gaatacatag atctctgctg gtttgatcaa cgctcgagca atacatatcc gctgtttctg
    12781 gccccctgaa taattagaac cgccttgagc aacaaagctg tccaatccgt caggaagggt
    12841 gtccactagt tcttctaatt gagcgatctt gatcgcttta gacaactctt ctgttgaggc
    12901 atattcattt cccatctgca agttgcttaa aattgtcccg ctgaataaga aagccttctg
    12961 agggacataa ctgatgtggg agcggatctg ttcttgagtc atttgagtaa gaggaatacc
    13021 accaagggtt acttttccta tcgtagggtc actaagtctc aagagaagtt tcgccaaact
    13081 actttttcca gatccggtcc cgccaacgat cgctgttgtt tttcctttag gtattgtgaa
    13141 atcaatatct tctagaactg gatcagagat ttcagtatat ttaaatgtga catgttcaaa
    13201 agcagctgca tttgtctgat caaaggttgg ttcagctact gttttgatcg tctcttcctc
    13261 aatttcgatc tctgcttgca gtatttcttc tatccgtcgt aatgaggcaa acgatttagg
    13321 gataatgacc aataccatag ctgcgatcat caagtaagca agtgtcagca tagagtattc
    13381 gactacagca gtcacgacac cgatttgtaa tgctccttct gctacaagat ttccgccaaa
    13441 ccacaaaata gaagcataaa cgattcccat gagcaaaaaa gcaattggag tgacccaagc
    13501 aaatattcgg ttcactttga tcatttgatt agcatagtca gcaaagctct tattcgttcg
    13561 cttttcttca tatgattgat tatcaaaggc acggatcata ttaatcccag taaagaattc
    13621 tctcaatgtg atggtgatcc gatccatttt tggctggata ctcaaagata aaggtgcccc
    13681 tcgtttcatc agtcgataaa caatccatac ataaaaaaca acagagatca atgtaaccca
    13741 tgttagtaat agagaataac gaaacatcat gagaatggtg aaaacgcaaa taaccggtgc
    13801 tggtaagatc atttggcaaa ataaaacaat catttgctgg acattatcga catcattcgt
    13861 cattcttgtc aaaagagatc cagaactaaa acgactgata tccttcattg acagctgttg
    13921 gaatttacgg aaatatttct cccgcgtttg gaatccaaac ctagcggaag ccaatgctga
    13981 aaagtaacta gaggcaactg cagcgattgt tcctagaaac gctacaagca gcatttcaac
    14041 tccaattttt tttataactg aggcttcgcc acttgctata cctgtgtcga tcagctttgc
    14101 tactaatttt ggtacaccta atgtaccggc gatttctaaa cagatcatca caaaggtaag
    14161 gagagcgagt cctttgtttt tctgtaagaa tgtttttaag atagacatat tattcctcct
    14221 tttttcctga tacttttcga ttctatacct tcaagtaact tgaaggtcaa tagaaaaaag
    14281 aataaactga aaaagaggtg agaaaaaatg aaaaaaatgt atacgattgg ccaagtatcg
    14341 gaactatttg atttgccaaa atcaacgatc cgctattggg atgaacaagg attaatccgt
    14401 tcaagtagac aagaagagaa tgattatcga ttgtttgata tcgatgatat ttttatgctt
    14461 tatgatatcg ttttttatag aaaattagat ataccgatca aacagatgaa gaatttatat
    14521 ggaaaaacat tgacggagtt atatgagaca cttgatgaaa cagaaagaag aatccataaa
    14581 gaactggtgg tgatgaaaca aaaacaaaaa gagataaggg agcaaaaaaa acagttgaaa
    14641 ttgatgatcg atacaaacga ggaagaattt cctgtagaag agatcccttt tgactgtatg
    14701 atctcaacgg agtttgaaga tattgtggaa atcaaaaaat ttctacctaa ctattcaagc
    14761 tttgggatga tgagtatgcc agcttccagt tcacagacga tgtatggatt ttttatcgac
    14821 cctagtgaag tacatttatt tactcaagac gtgatttggg agaaaaaaga tacggctgtt
    14881 tatcgtcgat tcttattgaa atccgagatg aaccatgcgg aacgtaataa tattttagaa
    14941 atcagagaaa gaatgtatga aaaaggatgg aaaacaggag aagtgatcgg ccagtatcta
    15001 ttgacgaata cggatgaaaa caatatcaga actgaatatt atcatgcatg gatagaaatg
    15061 aaaaaatagg aaatatgtgt ttaagataac tcgaaaatga tggttgtttt agttataaag
    15121 aaaaattttc tggttcgatt acgggtttaa tgtgggggat tattagtcgt ggatagatag
    15181 aattttagtt ttttagagga aatatgtctt taaaaatgga atggttgcaa ttcttagcga
    15241 tatatacgat gtaattgtcc tcttaaaaaa gtatggaaaa ataattgtat tgggtgttat
    15301 gtataattca tggtgaactg agactaatga gaagaaaaat tcatattgtt atgagtgaga
    15361 ttgtatcaga tgagaatgat aaattaaata ggtgcataaa agcattacac ttaaaataca
    15421 ttgttgctta cattattaca atatgttaat aaggcaaaaa taaatagatg gattgaataa
    15481 acatattttt taatcaaata gtccctactg tgactttcct cagctatcgg tgatactacc
    15541 gatagcattt ttttgcttat aaaataatta taagtattta gagatttttc ttctaacatt
    15601 cttcaaacaa gaaaaatcac aaaggttatt gttattttta tatattcttt ttaagaatga
    15661 ttgttttttt atagaaattt taaaaaataa aacacgttat tgatggatag ttagtttgtt
    15721 atttggtaat attatagaag ttgactggta gtgatttatt tttcgtcgtt ttataaaagt
    15781 gttatttttt ggaaaaattc acaaatatgt aacgaatgtt tttctggaat ttgctgtgtt
    15841 aaaaagcagt atgactaata taaatactat tgtcatttat gcaatttttt atggatagta
    15901 attttagagg ggaatcaatg aaggaagaat agaagggaaa aatagttgca aatacgccag
    15961 ttagcaaaaa tagcaataca tcttcaagct ttaaaattcg agagaatcat cttggtagtg
    16021 gaatcaacta caacaaaagg attaaaccaa ttaatagata gaattaccaa tggaaaaata
    16081 gaaaaggtag ttattctata caaaagttgt ttaattcgct tcggttatga acttagtgaa
    16141 accctttgca atatatggta cagaatatag aaattattga cattacagaa aatgatgaag
    16201 atttaataca gattgtcact atttgtagat accttttaca aggaaaccga gccaataaag
    16261 ctaagaagat gattaaggag ttgatggaag acgatacaag ttaagaaagt gaaactacgc
    16321 ccaaataaag agcaaaaaga gtaattggac gtggaaaatg caagaaatga accatcgatt
    16381 cggtggacag tttattagta atgacggttt gagaacatcc atgacgagaa tgaaggaaca
    16441 tcctaaatgc gtttagttga accaagtgcc aaataatatg gccaaataag cgatcagtta
    16501 tcgatgaatc ccaagtattc taaactatat attcattttg atgctaagca ttgatatatt
    16561 tttttgggat tgatgaaggt taaccaatac agggattcac tgatgaaatt ctagagtctg
    16621 accttggtat caacgattta gtgattgttt ctaatggaaa agtataaaag tatataagta
    16681 cattaacaaa ataactatta gggttacgct ccagcttgtg caaggcactg gcaggaggaa
    16741 tcgtgaaatc ataattatgg ttaagccaca cggacaacta aaagtcatac aaagctatcg
    16801 aatcaatcaa cagaagacaa tcggcaatca ttagctttgt ttacagctta cattgcaacg
    16861 cttgagttga accgaaaata cgagggaggt aacgcagcca ttttgagttg gggaatcttt
    16921 ttagaaaatt atcgggtacc ttgttgttta gtcaaaatta taagggggag taatagtgag
    16981 gaacaaattt cataaactgt taagtatgtt tctagttatg cttattgtga tcaatcaatt
    17041 tgggaacata acggttctag ctgagtcttt agcagaacag ccagagcgtt cgctgacgat
    17101 cgaagttgat caagaggagg ctgctttggg tgagcagttc cagttgcgag tcagaggaac
    17161 accagacctg ctaaatgcat tggtcattga taagagtgag gatttgattg aaggggaagt
    17221 gaagcaactc aatgattacg aacgtctgat tacttattct tcccagcatt caggtaaata
    17281 cgaattgacc gcaagaagcg gtgatattca atcaaatacg gtctcaatag aaatcagtgt
    17341 accagaagaa agcacaagtg agtcttttaa tgtaccagaa gaaagcacaa gtgaatcttt
    17401 taatatacca gaagaaagct taagtgaatc ttttaatgaa gccagatcat ctgagccatc
    17461 tataacgcag gaatctcaga ttgaaagaca agaggagctc ttgaattatg acatcggcat
    17521 ctctgctgag cgccccactt tgccgagcag gcatacacaa acgttaaagg gatacattca
    17581 aggaggcgct ggcgatattc ttgaagaata tggggagatg gtcgtacgag ttcctagagc
    17641 atcgattggc ttaattgagt tcattagagt ggaggtcgct gatgattcac ctttaaagcc
    17701 actggcattt taccaagaag gcacagacgt tgtttttcca tttacctttg attatgaagg
    17761 attaaatgaa gccgctcagt cgtatacatt cagtatcacg tttgaagttg ctttctttca
    17821 atcaggcggt actgagccaa ataatgactt tcaagtgagt cttacttatg ctgagcgagc
    17881 agcaaacacg gaattttcaa ttacgcctat tccttcatca agacccattt tttcgaaatt
    17941 atactatggc tctagagaaa tcgacagcca aggaaatctt gggaatctgc tgattgacgg
    18001 gcaagacgtg accaacagta ataaatttat tttgcaggta aattatgaag gatcacgaaa
    18061 agacggtgta gtggttaccg atgtactacc gcaaggaaca actttggcac cgaatccgac
    18121 tacatggagt tccactggaa atagagaacc tatttcaggt ggggttcgga tcgcagaagt
    18181 aacgaatcca ggtgaagcaa acgttagaac tcattatgtt tccgaggaat ttgtggacaa
    18241 agtatctttt aataaggaaa cgaatacact tgttgtggac tttggtaatc tagcagaggg
    18301 tcaatcgtat tttattgaat atgctcttga agtgaccgat gtcgacttgt tcaatgaagg
    18361 aagacaagag gatgggtttt ctcggaatcg agcgagtatg acctacaata atcaggtaat
    18421 gagccgagac taccgcttga gaatccctga aagtaatctt ccggcaatct ctttaagaaa
    18481 aagtgtggac aaaaatgtta tcaatctcaa tgaaaatgaa ctaacgtata ctctgcatgt
    18541 ctcgatgttt tctggaacaa gaaatacgct tgttattgaa gatgttttgg aagaaggcat
    18601 ggaatttgtg tcatttctat cagatattcc ctctttcatt gatgccgaag tcaataatgg
    18661 aatagttcgt ttcagtgcga ataatacgat tggtggtccg acaagtttta ccctctcttt
    18721 taaggtcaat gtagaggcgt atgcggtcgg cgatcaaatt ttaaactttg caacgcttgt
    18781 gaatagtgac aatcgattga atacgagtac tgtaagaaca aggaaaatcg atgggcgtat
    18841 taaagtaatt aaaactgatg agaatggcca aaggctggca ggagcaaaat ttgaaatttt
    18901 atcaagcgat tcacaagtga tacaagaagg tataacggca gaaaacggag aatttttaag
    18961 tgaaccgctt cctgtaggta gctatcaagt ccgagaaaca gaagcaccgg aagggtatct
    19021 tttagatgcg acggtacatg atgtttttat aaatgaatac tccatagaag aagtgacgtt
    19081 aaccattgaa aacatccttg atacgggtgg tgttgaacta attaaacatg ctgaaggcag
    19141 tggtgaagtc cttcaaggag ctgtttttga gttacagaac cgagaaggag aaacgctaca
    19201 aaccggt
//
LOCUS       DACDTH010000055        19166 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDTH010000055 DACDTH010000000
VERSION     DACDTH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.055"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..703)
                     /locus_tag="IWK54_002039"
     CDS             complement(<1..703)
                     /locus_tag="IWK54_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9594154.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="IWK54_002040"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="IWK54_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="IWK54_002041"
     CDS             1758..2837
                     /locus_tag="IWK54_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9594155.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="IWK54_002042"
     CDS             2869..3462
                     /locus_tag="IWK54_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9594156.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="IWK54_002043"
     CDS             complement(3572..3799)
                     /locus_tag="IWK54_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9594157.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="IWK54_002044"
     CDS             complement(3902..6490)
                     /locus_tag="IWK54_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9594158.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="IWK54_002045"
     CDS             complement(6510..7169)
                     /locus_tag="IWK54_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9594159.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IWK54_002046"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IWK54_002046"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9594160.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="IWK54_002047"
     CDS             complement(7942..8808)
                     /locus_tag="IWK54_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9594161.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="IWK54_002048"
     CDS             8917..9558
                     /locus_tag="IWK54_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9594162.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IWK54_002049"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IWK54_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9594163.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="IWK54_002050"
     CDS             complement(10999..11898)
                     /locus_tag="IWK54_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9594164.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="IWK54_002051"
     CDS             complement(11900..12697)
                     /locus_tag="IWK54_002051"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9594165.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="IWK54_002052"
     CDS             complement(12675..13379)
                     /locus_tag="IWK54_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9594166.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="IWK54_002053"
     CDS             13510..14085
                     /locus_tag="IWK54_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9594167.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="IWK54_002054"
     CDS             14203..14880
                     /locus_tag="IWK54_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9594168.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IWK54_002055"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IWK54_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9594169.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="IWK54_002056"
     CDS             complement(16943..17941)
                     /locus_tag="IWK54_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9594170.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="IWK54_002057"
     CDS             complement(18167..18820)
                     /locus_tag="IWK54_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9594171.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5532 a         4144 c         3331 g         6159 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagtcttt tggctctata
    19141 ctttttgatg ttggtgaact tcacca
//
LOCUS       DACDTH010000056        18947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDTH010000056 DACDTH010000000
VERSION     DACDTH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.056"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            92..1519
                     /locus_tag="IWK54_002058"
     CDS             92..1519
                     /locus_tag="IWK54_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9594172.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1611..1976)
                     /locus_tag="IWK54_002059"
     CDS             complement(1611..1976)
                     /locus_tag="IWK54_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9594173.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1973..2338)
                     /locus_tag="IWK54_002060"
     CDS             complement(1973..2338)
                     /locus_tag="IWK54_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9594174.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2530..2778)
                     /locus_tag="IWK54_002061"
     CDS             complement(2530..2778)
                     /locus_tag="IWK54_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594175.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2777..2905
                     /locus_tag="IWK54_002062"
     CDS             2777..2905
                     /locus_tag="IWK54_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9594176.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2962..4656
                     /locus_tag="IWK54_002063"
     CDS             2962..4656
                     /locus_tag="IWK54_002063"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9594177.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4756..4929
                     /locus_tag="IWK54_002064"
     CDS             4756..4929
                     /locus_tag="IWK54_002064"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594178.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5307..6440
                     /locus_tag="IWK54_002065"
     CDS             5307..6440
                     /locus_tag="IWK54_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9594179.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6471..6791
                     /locus_tag="IWK54_002066"
     CDS             6471..6791
                     /locus_tag="IWK54_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594180.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6820..7284)
                     /locus_tag="IWK54_002067"
     CDS             complement(6820..7284)
                     /locus_tag="IWK54_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9594181.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7416..8669)
                     /locus_tag="IWK54_002068"
     CDS             complement(7416..8669)
                     /locus_tag="IWK54_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9594182.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8698..9015)
                     /locus_tag="IWK54_002069"
     CDS             complement(8698..9015)
                     /locus_tag="IWK54_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9594183.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9041..9361)
                     /locus_tag="IWK54_002070"
     CDS             complement(9041..9361)
                     /locus_tag="IWK54_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9594184.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9825..11240)
                     /locus_tag="IWK54_002071"
     CDS             complement(9825..11240)
                     /locus_tag="IWK54_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9594185.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11257..12573)
                     /locus_tag="IWK54_002072"
     CDS             complement(11257..12573)
                     /locus_tag="IWK54_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9594186.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12723..13421)
                     /locus_tag="IWK54_002073"
     CDS             complement(12723..13421)
                     /locus_tag="IWK54_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9594187.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13710..14699)
                     /locus_tag="IWK54_002074"
     CDS             complement(13710..14699)
                     /locus_tag="IWK54_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP9594188.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14779..15612)
                     /gene="msrA"
                     /locus_tag="IWK54_002075"
     CDS             complement(14779..15612)
                     /gene="msrA"
                     /locus_tag="IWK54_002075"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9594189.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15787..17421)
                     /locus_tag="IWK54_002076"
     CDS             complement(15787..17421)
                     /locus_tag="IWK54_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594190.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17408..18808)
                     /locus_tag="IWK54_002077"
     CDS             complement(17408..18808)
                     /locus_tag="IWK54_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9594191.1"
                     /translation="MWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIY
                     YRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIA
                     CMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTD
                     LQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTW
                     VCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGA
                     TVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERP
                     HLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVND
                     SYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKD
                     SRYVTKKEINKKNENK"
BASE COUNT         6009 a         3724 c         3161 g         6053 t
ORIGIN      
        1 ctttttcaga aattaactga tgatttcaaa aagaaaacat gtcataatat ttcccatatt
       61 gtgcaaaaaa atacaaggcg gaggctctac tatgattgaa gaatatattg agaaaaatat
      121 tttaagacag ctgtttttat gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa
      181 gctctccaat ctcctgcatg tttgcaaaac cactttactc aatgatatca ataacataaa
      241 aaaagaattt gaagaacaga tcgcttatac acatcgggaa aaggaccgtt acactttata
      301 tttttcagaa catatacctc gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt
      361 tttaaaaact tgtctgcttt atttggaaga agatgaacct gattatcttc aattaactga
      421 atgtgaattc atctctgtca gcaaagctta ttcattaaaa aaacaagtat tagcttactt
      481 taatgactgc gggatcgaaa tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg
      541 tctgctgctt cttaatgtgt cgtatcgttt aggcggtttt aattcttggg agctacctga
      601 atcgtttttt gaacgtgccg atcgtttcat tgaatccgta acggaaaata gtggcagatt
      661 ttatgataaa gaaaataaag aaatcttatc gattggtttt gctatcagtt tcctacgcca
      721 gcaagtgtgt gctgtgacaa tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat
      781 ttacaattat gtcgaatcag catgggaaaa cactgatttc caaacctatt ataaaaaaga
      841 agaattcgcc tttattttga cactattcaa tctttgtaat tatggtttcc attcttatca
      901 gttgattgcg gaagactttc aacagctaca ccaagtgttt attgacaata ctcccgaaat
      961 aaaagaacta gttgctacct ttgaatctca cttcaatcaa gaattattcg gaaaccaacc
     1021 gttcgaacgt gcgctgattc atctgatgcg ttcggcttgg gataactatc aattattcat
     1081 gccagaaaaa ttttatcttt taaatgagga acagacaaat ttatataaag aagtccagac
     1141 aattttttcc agctggtcca gccagttacc ttatgatcta cggttgaatc ctaattgcat
     1201 gcgggctttt gtgatcgaat tgagcggaat tctccgtctg acaaaagaac atctgactat
     1261 ctatatcgtc acaaattcgg atgttcatta tctgatttat cgggaagcct tggaagctgt
     1321 aactactttc gattttcaag ttgcaccaac aatttattca agtatatctg atataaagaa
     1381 gtatgcgcag cagtcctcaa accgggtatt atgtgaacgt acattatata caccagatgc
     1441 ggtccaatat gaaaatatta ttcctatttc gatcaatacg atcgatcgag ctatcatttc
     1501 agctgtgcaa aacaaataag gggctgtgac aaaaatcatg tcacagcctc ttatttgttg
     1561 aagatgccca tttctgtaac aatctcttga tacacgtcag gctcacaacc tcacttattt
     1621 tggacatcta ggggtccgat ctctttattt aaaaaataag aaatgatggt tcggataact
     1681 accaacgccg ctagttggaa aatatcctgc aaggtaggtt tgataatcga ttcgatgatc
     1741 ccagctgaaa caagaatttc caaacttaga agtatatagc ctcccaacat ctttttaata
     1801 gcattgttct cgcttactcg gtcgctttgc acttttgtat ggacaaataa atatttcaaa
     1861 aaatccttga ttgatagaca aacaccccaa accaataaac agatagcaaa gatatcaagt
     1921 cccagtacaa gtttttcgaa atatggaaga agaaactgca tcaactctgt cgtcatccta
     1981 cctctcctct tctttgtttt tctttgtctt ctatttcctg attcaagaaa taagaaatga
     2041 ctgttcgaat gataataata agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa
     2101 ctgactcgat gatatcagca acgactagta cttccaagct taacaaaata taacttgcta
     2161 gcagttttct tgtatctgca ttacgagtat tacgttctgc atagtttttc ttgacatgag
     2221 accaatcaaa aagtttgaat agaacaagaa cgatcccgac aattaagata ccgatagaac
     2281 aaatatttaa aatagcaatg atccattcta gaaattcaat taaatttgag cgaaacatat
     2341 acgctaccta ctttctttta atttgttcct attcttttat tgtaaaagaa agttaccttg
     2401 ttcgtctatt taaaagttgt tctaatgata acggttcgct taaatttttc tttttcactc
     2461 tcccattttt ctccctccat ccataaatcg tggaagtatc caaaacaacg tagcaagtta
     2521 aaataaggct tagtccgtca tcagcttttc aaagaagcac tgtctcggtg ctttttttgt
     2581 acccaaattt ctaaaaatag aaccatacac ctcctcaaat cttttcttct tacctaccct
     2641 atttttattt acaggaaaat cagacacttc ttcttttttg tcaaaatttc ctttttcttc
     2701 tcgtcctatc atttggcatt ctaataaaaa cgaactatcc ttggatatag aaagagaaaa
     2761 caaggaggcg tttttcatga gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct
     2821 tgcccttggc gctgcatatc taggaaaaaa gacaggacta ttcgaagatg acagtcattt
     2881 atatgatgaa ttcgaatcaa tctagcatcc tatttagttg aatcactacg gacatacttg
     2941 gaagacattg gaggaatcac aatgagaaaa agtaaagcaa taatgattgg tgccggtatt
     3001 gccaatatgg cagcggctgt ttatttgatt caagaaggca aatggaaagg ggatcagatc
     3061 accttttatt cattggatga tcacggttca aatgatggtg caccgaccga cactgtagtg
     3121 gatgaatatt ggaataaaaa ccatccatta gaaaaccaaa aaggctatat tgctcgaggc
     3181 ggtcgcatgc ttaattaccg tacctatgta gatttgatgg atctgttaag tcgaatacct
     3241 tctgttactg agccagggat gactgctgaa gaagatacga gagattttga tgcaaaacac
     3301 cgaacctttg acaaagcacg tttgatggaa ggtggcaaag gaatcattga tggcggaaaa
     3361 ctgggattca ataataaaga ccgtattctt ctgactaaac tgattgctat gcctgattca
     3421 gaagaagaaa aactagacaa tatcactatc gaagaatatt tcaaagatga tccacacttt
     3481 tttgaaacaa acttctggtt tatgtgggaa acaacatttg ctttcagaac acgtagttct
     3541 gctcaagaat tgcgtcgcta tatgcatcaa atgatttatg aattcactca aatcgaacat
     3601 ctagtgggcg tcaatcgaac acgttacaat caatacgaaa gtattatgct tcctttgatc
     3661 gagtacttga aagaacaagg atgcaaaatc attttgaacc gccgtgtcac tgactgggaa
     3721 ttcaaagaaa caccgatgca agatgaaatc actgtgactg gtttgaaaat taccaataca
     3781 ctgactcaag aagaagaata cgttgtggtc gatgaagata cagcagttat tttcactaat
     3841 gggtccatta cagattcagc tactttagga gattacgaaa caccagctgt agaaaatatg
     3901 gattatggtg cagcttctag tctctggaaa aaagccagtg aacgtttcta caacttggga
     3961 aatcctgaca aattctttgc tgatcgagat gcaagcgaat gggtcagctt taccctgacg
     4021 acgaaaaacc atttgttgtt gaatgaaatc acccgtatca cgactcaaga accaggaaat
     4081 gccttgaatt catttatttc aaccacaccg atcacgccgt taggccaaaa agatgtcaat
     4141 atgtctattg tcgtgcatca tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt
     4201 atttggggtt atttccttta tccaagacga cgtggcgaat ttgtggataa acagtatatt
     4261 aaaatgaatg gaaaagaaat gctagaagaa ctgattggac aactttcaaa agtagatcct
     4321 ggaccagtga atattcgtga aaaagaaaca gaaattttcg acagcgtaat aaataatatc
     4381 cctgtctata tgccttatgc ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca
     4441 aaagtcattc ctaaacattc gaccaatctg gcctttacag gagaatttgt cgagcagcct
     4501 tatcagatga tctttactga acaaagtgct gtacgttctg gagaaatcgc tgctttccat
     4561 tttgcgggta ttcctatgtc ccgtcttgtc aaaacaccgc gttacgacaa agatattcct
     4621 actctagctc gtgccgcaaa aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc
     4681 ctgcatcaag taataagaac aaccaggcta aaacagcttc ttatgttttt gatttggttg
     4741 gacttattac cgcagatgca agcttttgaa caccgtttac caagaggtta tgaaaaagct
     4801 gccctgcatc aagtaataag agcagccaag ctaaaacagc ttcttatgtt tttgacttgg
     4861 ttagacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa
     4921 ggcgtttagt ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat
     4981 aaatttgact taataccgag aaactgatcc cttctgtcat aacctctttt tatttatgtc
     5041 tgtttctttt tgaaaacaac tattttttca acgtttactc tcttgccaat ccaacatcat
     5101 ctaacggata caactatttc aatgattacc attacactaa aaaatcggaa acacacctct
     5161 ccgaacacaa atacataaga ctatagaatg tttcatttaa gaaaaaaagc gtttcattat
     5221 caaaaaggta agatctcctt taaaatgaaa ttatctgtag tactaataat gcagacaatt
     5281 ttatgaatct gaaaggagca tctccaatga aagcagtcac ttggcaagga aaacagaaaa
     5341 tggaaatcag aaacgtagat gatccaaaaa tattagaacc aacagatgca atcatccgta
     5401 tcacagcaac agccatttgc ggctccgatc tgcatcttta ccatcatggt gaagcagttt
     5461 tggaaaaagg gtatgttgta ggacacgaac caatgggaat cgttgaagaa gtgggatctc
     5521 aagttcaaac attgaaaaaa gggcaacgtg tggttatccc ttttaatatt agttgtggtc
     5581 attgtcattt ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat ccagatcagt
     5641 tatatggcgg gcttttcgga tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg
     5701 aatatctaag agttccttat gcagactcta cttcatttgt tgtaccagat aatgatcttc
     5761 ctgatgaaaa agttttattt ctatcagata ttttgcccac agcttattgg agcgttgaaa
     5821 acgcaggagt aaaaaaaggc gatacagttg tgattctagg ttctggtcca gtcggtctat
     5881 tcgctcaaaa attcgctgtt atggctggag cagagcgcgt aatcgcagta gatccagtac
     5941 agcaccgtct agataaagcg gctaattaca atggggttga aacttatttc ttagaagaca
     6001 cggcacaagc tggaacagaa ttgtatgaac tggctaaagg tggcgcagat gtcattatcg
     6061 attgtgtagg aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct
     6121 tacaaagcgg cacaatctct ccattacaga tggcatcgca agctgtcaaa aaatttggaa
     6181 cagtccaagt aactggtgtt tatatgactc cagcttcttc ttacccgctg caagaatttt
     6241 ttatgcggga tatcgctgtc aagcatggac aggcaccaat tatccatcta atgccaaaaa
     6301 tttacgcaat gattgcagaa ggactattcg atcctacaca aatcattact cattcgctgc
     6361 cactatcaga agcagcacaa gcatatgaga tctttgacaa aaaagaagat aaaaatataa
     6421 aagttgtttt gaaaccttga attcagtaaa aaatacaggt gattttcttt atgcccaaaa
     6481 tcaaacgaaa tacccatgag atcgaaatag gaaaagaaga agatatcgaa atacgaggac
     6541 gtcgcttccg cttctatttc caaaatcgtt acacattgat ctcactggct gtcgatttat
     6601 taaccgggat tttctatatt tttggaagcc ttgcttcctt aactaaaatc cccgatcgat
     6661 atggcaccta tttttatttg gctggtgcta ttttcttgac gattcgtcct attctcagaa
     6721 tcgtaaggaa catttttatt tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa
     6781 aagaggcatg agaatttttc aaatctcatg cctctttttt caccctatcg tatcactttg
     6841 atacagacga gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg tccactcgta
     6901 tcgttcataa aacgtggtat gatctgtaat taagtaaagt ttttcgtacc caagttgatg
     6961 catttctttt ctgatttccg ctaatatccg cttggctatc ccctgcttac gcttttccgg
     7021 ttcaacaaac agggcacaaa gatttggtgt cagatcaggg cggttatgga agtcgttttc
     7081 aatgacgccc gctcctccga cgatggtttc tttttcatct aagaccacat accattgagg
     7141 aatactttcc ttattagcga ttccagtacg aatactttcc tcatacacaa cagcaggaac
     7201 tccccatttt gatgaaaacc aattcgcgct ttgttttact aattctgggt gttctcttaa
     7261 tttgatccag ttatatgtaa ccaatgttta aacctccgac taaaataaaa cgtttacaaa
     7321 aattatacaa gtctttatga aaattagcaa tgacaacaat agtgatgtaa aaaaaaaaac
     7381 ggaaaacaat cacttgttct ccgttttttg attaatcaag catctaaagc ttcttttctt
     7441 ctttcttgga tcgtagctac cttgacgaac ggtagataga tcaaaataga aagaaccaaa
     7501 ttgacagcgg ctagtacacc acctgcaata ctttttgttg ccaatacacc gccaataatt
     7561 ggtggagtta cccatggagg cataacggta gctgctggca ctaaacctgt agaagtcgca
     7621 aagtaagcaa cactaactaa gaccattggt gtcaaaataa atggaatgaa catgatcggg
     7681 ttcaacacga taggtaaccc aaacatcgtt ggttcattaa tattgaagac acctggcgca
     7741 atcgacaagt ttgttacaac catatatggt ttgtttttac gtcctactaa ataaatagct
     7801 aacagtaaac ctaaagttgc acctgttcct cctaagttga caaagctgtc aaagaaaggt
     7861 ttgttcacga taaacggtgc gctatgtcca gctgcgattg cattaacgtt tgcttcaatg
     7921 gctggcgcat tgattgtttg catcaaagga tcgaccatat tcgctccgtg taaaccaaag
     7981 aaccataaga atggtgtgat aaatgcaagc agtaaggcag atggataaga gtttgcaagt
     8041 cccataaatg gttcttggac cactgtatag aaagaaccta cgatatcatc aacaccgaat
     8101 gcagcaaaaa ttgcagcaac taaagcaaac gcagagatag tgatcattgc tggtagtaat
     8161 gaagcaaatg cttttgcaac tgccggtggc acaccatcag gcattttgat gatcaatttt
     8221 tcatttccgc ttaaacgtac aaagatttct gttgaaataa tcccaacaat aatagcaata
     8281 aacaaaccgt ttgagctcat acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa
     8341 atcacacctg cggaaacacc gtcttttcgg tagccgttag ctaaatgata agcaagcaag
     8401 aaagcaatca agacagaaag aatggcaaat gtaccgttcc aagcagcagc cccgaatcct
     8461 ttccatgatt caccaccaaa aattgagttc atcaattctt ggaacccggg aatcggtaag
     8521 ttattgatca ttactgctaa tgctcctaag atcattaacg gcatactcac cataaatgag
     8581 tcccgaatcg caactaaatg ccgttgtgca ccaatttttg atgctacagg tataaaatgt
     8641 ttctccataa atgcaacaaa tttatccata aatcttccct cttttcttta ctgtagatta
     8701 cgcgttcaat cgacgataca gattaatgat ctcttttgct aaatctgtaa aagctataga
     8761 tgtcatcaga tgatcttgac tgtggaccgt caaaagcgtg acttcaacgt gatttccttg
     8821 tgcttcttgt gtcagcatct ctgtttgaga gtgatgtgct tggactaaag attcctctgc
     8881 atctttgatt ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc
     8941 cattgcatcg ctttttgcat ttccgccaaa catgatcaga cccatgatgg cttctagatt
     9001 tttttgatct tccatttatt gtatcctccg ataattttta ttatttgttt attagttttt
     9061 ctgcttggtc gagtactttt tcaccattca tcatgccata gtcagccata ttgattacat
     9121 ctaaaggaat gcctttaggt gctagctttt cagcaaattg attttgcata aagcgtactt
     9181 gcggtcctaa aagtaaaacg tctacatttt tagattctag ttgattatcc gcatctgaag
     9241 cagatacagc aaagatgtct gcttccattc cttttgcttc tgctgccttt tgcatttttg
     9301 tgactaggag acttgtactc attccagctg aacatacaag catgattgtt ttcttttcca
     9361 ttttaaacac gttcctttct atacttacac ttgtatataa gcaagtagcg tgccaacttt
     9421 aaaagcgctt taaaaagttg atatcatcac atttaaggtc tataccaagt tgcatacaca
     9481 ctaaaaaaga aagcgcacac taaacacaca aattaatttt tattaccttt tttcttttaa
     9541 cgtgcacaaa aaaagttgtg acacaaaact tgtcacaact tttttcaaat aaacggtatt
     9601 caaaagcttg catctgcggt aataagtcca accaagttaa aaacataaga tgttgttttt
     9661 actttgctat tcttattact tgatgcagga cagctttttc acaacctctc aataaacggt
     9721 gttcaatcag ctgaccctgg gtattagctc aaaacgctgg attcacaacc tcttttattt
     9781 caaataacca gtaaatacaa tgaacatcta caaaaaactt ctatttattt atagatttcg
     9841 tcaggcaaat ccaattctct cttatcaatc aaagtctgat accacgaagc agattttttc
     9901 aatgttctgc ttcgttcatc gttcaattcg attcgaacta atccatatcg attcttaaat
     9961 gcattcattg gtgacacaca gtcagtgaat gcccataaca tatagccttc gcaatttgcg
    10021 ccttcttctg ttgctttgat caagtaatgc aaatgctgtc cgataaattc gatacggtaa
    10081 tcatcttgca cgattccttt ctcatccata tactgttctt catattctct tcccataccg
    10141 ttttctgtta tcagccaagg gatattatga taattttctt ttagatacat ggctatatca
    10201 tacatgattt tcggatagat ttcccatccc ctggaattat tcattttctt cccaggtaaa
    10261 tcaaagactt cataatatcg ttcaggatgg aaaggtgtct cttcattcca ttgataacga
    10321 ggggcgcgaa cgcgtttagg ataatattga ttgattccaa ggaaatctac tgtatgatgc
    10381 tgaatcgttt ctaaatcctc tgttgcagga tcgaaataaa tcgattgttt ttgacacaac
    10441 tccaggaact cttctgtgta gtatcctttt accatcggat caaaaaatac tcgattataa
    10501 aacaaatcat acatataagc agctttttgg tcttctggag aattggatcg agcatagacc
    10561 atttcaggat ttaaaataca gccaatcctt ccaccataat ttccgctgcg gtaaatctct
    10621 actgctttag ccgttgctaa aactttatga taattccaca tcatccactt ttttgtgttt
    10681 tgttcatgcg gccagcgaac agcatccaaa tagcaacgcg tttggggaac gattggttcg
    10741 ttgaatgtaa accagttttt taccctatca ccgtaacgat caaaggcaat ttgagcatac
    10801 ttagcataaa aatcaactac ttttcttgaa gaccagccat catatttttc caaaagaaaa
    10861 gctgggactt cataatgttc caaacaaatc atcggttcta cgtctgcttc gatcaaagag
    10921 tcgatcattt cacttatatg ctgagcatat tcttcatcta ctatacagtt ctcataatca
    10981 gtaaaaaaac gagcccaatt gatagaagtc cgataatgtg tcagatggat ggatttcatc
    11041 agatccacat cctgctggta ttgttccata aaattagttg ctacggctgg gccataccca
    11101 ttatgccaaa caaaatgttc atttttatac cagctatcta taaacgaatc ctgtccttct
    11161 ttttttcctt tccaaccttc cgtttgccaa gcagaagatg cagcaccaag aataaaattt
    11221 tttggaatag taattttcat gatttcctcc tgttttttag atcccatcat tttccacacg
    11281 atttgctact tttacaaatg gcagatagat caaaatagaa acaataatac aaacaagttg
    11341 cgtaataact gcaccgatat ttcctgctgt tgccagataa gcattgacga tcggcggcat
    11401 tggccaaggg accattacaa ctgctttcgc cgcaaatcct aaagcggttg caaaataacc
    11461 aatcgttcca gtgataagag gcgttaagat aaacggaata gctaaaattg gattcagcat
    11521 gatcggcata ccaaaaataa ctggttcatt gatgttgaag atcccaggag caagggataa
    11581 cttggtgatt tgtttcatat cttcccgttt tccaactaac aatactgcaa tcagtaaacc
    11641 gatcgtgact ccagacccgc ccatactcat atacatatcc caaaatggca tagtaataat
    11701 gttcggtact tcttttccag cttcaaaagc ttccgtattt acagcaattg ccgctaaaag
    11761 caacggttcc cgtatcggct tcaccatttg atttccatgg ataccaatca cccaaaagac
    11821 ttgcgcaata aacatcaata gcaaaatacc cggtaaccct tgtacgacac cttctaatgg
    11881 gcgttgtacg acattgtaaa tgatatcata aaggtacatt cctgtaagtt ctttgatgat
    11941 aaatcccgca gccgcgatac ccgatacagt cagaatcgtc gggaataaag cagaaaaact
    12001 agcagaaaca ttcgttggca cggtatcagg cattctcagc tgtagtttct tctgtttcct
    12061 caaccagcaa taaagttcaa tagaaaggat ggagaccaac attcccaaaa ataacccttt
    12121 cgtatctgta tattgtttag ccaatacgtt ttctactata acagtctgat tttcattcac
    12181 taattcataa acagttggat taacagataa ataagacatc actgcgatga taccagggaa
    12241 tacaccatcc actttattca gcttggctaa ttcgatccct atcagaaaaa cagcatagat
    12301 ggtcaagaaa cttagtgtga catagctgat ggcagatgcg ataggcttta attcttctaa
    12361 aaaacgcaag aaacttatct gtgctaagcc attttccgag tcaaagacca ttgctgaaaa
    12421 caatgtgcca aaagcacccg taataattac cggaatcaat gcagcaaatg cgttttttat
    12481 caccatgata taccttaaat tattgattct cgttgccacg ttccctaaaa cattggctaa
    12541 tttatccata aaactcattc cagatcccct cattcctgct tcttctcctt gacgtcatct
    12601 ctttttgtct attagtcagc gcttacattt cgatgaacag aaactattta ctcctctttt
    12661 ttaccaaata taaaacaaaa attcatctga tgtatttatc ataaaataca tcagatgaac
    12721 tgtcaattag tttctttttc tcctattatt ttctgtaatt ctctccgatt tattgccata
    12781 tggacagtca ttgcatcaaa cgcagcaagt gcattccgcc tttcgatggc ctgaacgatc
    12841 tctgtatgaa tatcgatcgt ctcgattttc agatgctgga tatcataatt tttattaaac
    12901 aaagcaattg attcattaat gattgggata atatgcagca aggctacgtt tccgctagct
    12961 tctgcaatca tcgtatgaaa atcgatgtct aattgaacat gttcttgtgt gccttctcga
    13021 aaagaagttt caatcttttg cgttaatttt ttcatttgga cgatttgatt cttcgttgca
    13081 tattccgctg ccaaacctgc catcttaggc tccaataaat atctcagttc aaataagtct
    13141 cgtatcatcc gatcctgatc ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct
    13201 cgcttatcac tgacaaatgt ccccgctcct tgccgaactt caaggatatt cctggaagct
    13261 agagcccgca cagcttctcg caccgtgctt cgccccacct ctaaataatc ggacaattca
    13321 tattcattcg gcagtttatc tcctacttgc aactgcattt cctttaaata tctaaggatc
    13381 ttgtcagtag ttacttctac tagggtttta ttttcttcca aaccacatac ctccttttca
    13441 tctgatgtat tttttcgtaa ttattataac acgtttgaat catctgatga ttttttgtat
    13501 acaaaattga ttaatccgtt catagacata caaacgaaga agatatttac cataaaaaag
    13561 actaggataa aattcactcc tagccttcga tcatttttat tttgaacgac aaaaaatatg
    13621 atgttcaata acagacgatc ttcattcgat tcttgatgtc atcttttgtt ttttttaata
    13681 tatggataaa aagtttgatt acatcgtttc tattctagaa tttggtcgat tctcaattct
    13741 tcttctggtg agaaatctaa attatccatc attcgaacac tttcttgtaa ctgacttaac
    13801 cgacttgctc cgaccagtac acttgtaaca gatttttgtc gcaaattcca tgcaagggcc
    13861 atttgcgcta atgattgtcc tcgtgatacg gcgattgttt gcagtgcttt gattttttcc
    13921 agagttgatc caacttgttc ttctgaaagg aatggtattt ccttacgatg tgctctagaa
    13981 tcttcaggaa taccatgtag ataacgattt gtcagcaacc cttgagcaag cggactgaac
    14041 gtgattgccc ctagcccttc ttcttctaat actggagata gatcgtcttc tagtagtgca
    14101 cgagaaaaca tattgtagcg catctgatga ataatgaacg gtgcttcctt tctttttaaa
    14161 atttcagtca ttttttttgt atcttctcca ttatagttgg atataccaat atataatgct
    14221 tttccttgtc ttacaagttg catcaaagct tctgctgttt cttccaaagg tgtatcaggg
    14281 tctgggcgat gatgatagaa gatgtcaaca taatctagcc ccattctttg gagactttgg
    14341 tcacaacttg aaatgatgga tttacgagag ccccattccc catatggccc ttcccacata
    14401 tgataacctg ctttgctaga aataatcagt tcatcacggt aggcttgaaa atcttctcta
    14461 aaaatacgcc caaagttttt ctctgcagct ccagcagggc ctccataatt attcgcaagg
    14521 tcgaaatggg taattcccat gtcgaatgcg cctcttaaaa tctctctttg attatggata
    14581 ggatcataat caccaaaatt ttgccataat ccgagagata aaatcggtaa tttcaaacct
    14641 gaatgaccgg atttacgata ttccattttt tcataacgtg cactatctgc aatatacaat
    14701 ttttctcctc ctcatcaaat ctttttaaaa cgctttcttt tttctatttt tagtttacta
    14761 cattttaaag aaaaggagtt aatcaaaatg attttttcgt atattttttt gccatttgtt
    14821 ttttactcgt tgatagatca gaaattgatt acgtgcttgc tggatttttt tataacgttt
    14881 cggttgtttt ttgtaaaatt gctgatgata gttttctgct ggccaaaacg taccggctgg
    14941 ttcgattttt gtgacgattg gttggtggaa agttcctgaa ctgtctagtt tctgttttga
    15001 ttgttccgct aattcttttt gtcgttcgac ttgataaaaa atcactggac gatattgctt
    15061 ccctctatct tgaaattgtc caaatgtatc tgtgggatca gtaatttgcc aatagattgt
    15121 cagcaactcg ctataactta tttctcttgt gtcataaatg atctccacag cttccacatg
    15181 acctgtgtaa ccaccactta cttgatcata atttggtttt tcaacatgtc cgcccgtgta
    15241 tcctgacaaa acagatagga tccctttttt cgattcaaac ggctcgacca tacaccagaa
    15301 acatccccct gcaaatactg cacgttcttg atagtcagca tcttgcatgg catgcttgga
    15361 aaagtcatat ttttgctcct tatcaaatcc ttccgtaatc aataaataaa agtccattac
    15421 atcaggcgtc agattcattc ggatagctaa tggacgtagt gttgcttcta gttttgataa
    15481 ctgttcttta acggatatat tttctcttat gtcattcttt gtctgaatca atacttttcg
    15541 ttcccaatct cgtgtattcg gattcaaaat cagattataa agtgtttgaa tgaatatttg
    15601 ttcctcatcc atagattctc ctcctttccg aagaagacaa gctagtgact ttagctgtaa
    15661 tttatcataa agtgaggaaa taagcgatta cttcatcccc ctacagattt ttcgtataaa
    15721 aaagagagaa tccttatcga cagattctcc tttttaatca atttatttta taaatgaaaa
    15781 gttaatttac cttttatgga aaactatttt aaaaaataaa cataaatatg cagttaatgt
    15841 aacaagataa gaaataatag tcgttactgt ataacttgtg cttactttaa tatcagcaac
    15901 tcctttgact ccgcttattt gtaccaatcc attagcattc acctgctcct ttacaggttg
    15961 atgatttatt tctacattcg ttcctaaata agcatataca ggaagtgtga ttacttggct
    16021 cttctcatta gttactctat atatgataga actaccagtt gttttataag gaaggtttac
    16081 ccattgactg ccaatctttg cttcgtgttt tattatatgc tccttattct ccaatgaagc
    16141 gataggagaa taatcatata aaaaataatc cattccttta gtggtatacg cctctactgt
    16201 ttctttgttt acaacaatat gtcccggctg agaaattatt ttttgattga aatttttttt
    16261 actagaaaca gttaaagtga ttaataacaa aacagtcata aataaataaa atagttctct
    16321 tgtcttattg tatcttctat ttttgaaaaa tatcatcgtt aatatcagag aagagaatat
    16381 tatttgcaag cttaagatcc gccaaggaaa ttgtaacaat ttggctggcg tattttgcag
    16441 aagataccat ggcaccaaac tagttgtagc tagccaagtc aaacacgtac cataataaat
    16501 ctttttttct atgtttgtta attttttaaa taaaatcagg ggaatacata aagataacag
    16561 accaactagt cccattgagt aagtagttaa gtcgttgttc atagcttcag tcagactatc
    16621 gaacacatta gatgcatgag catataaatt tgtcacgcta ggtttgttga ttttttggta
    16681 gagcatttgt tcaaacatcg gataccaaaa agcactcgtc agaaccaatg tcattcctgc
    16741 cgcactaaat aattttttaa atatagcaat aaaatctgca ttcttgaaac ctttagaaac
    16801 caaacgaata aaaataaaaa tgataattaa tgctgtactc attaatgttg atagtacatg
    16861 gctgtacact agcaaactca tactcaatgc tagctttaca gacggatact tttcttcttt
    16921 tccagagatg atttgataag cataaaataa tacagggata acaagtgtta ttgcgatagc
    16981 ttcggcaatt gcggaacgat agtagatgtc tactgtccga taagcagaga aaatatagag
    17041 acaactaaat aaaaatgccg cttttttttg ttttaaaaac cgctcgccat aatgataagc
    17101 tacgagtgtt gtacacaagt tcaataacca aacgtaaata atatagctta caatcaaatt
    17161 ttttgtcatc caatataaca ttacagctgg aaaaaaagtc agaaagggat aaaaataatt
    17221 tacaccatat ccagtataat tgaatgtctc ataattaatc ggactggtaa aaatagtcga
    17281 taaccccttg attcgagata agtgaaaaga aagatctcca gtggtataaa tactctgtgt
    17341 atttttgaaa aagatgaaag aatatatact actaactata attaatccac tcaatattac
    17401 tactttttta tttattttca ttcttcttgt ttatctcctt tttagtcaca tatcgagaat
    17461 ctttcaactt attaggtgca aatcgtttaa aacttgaata caccactaaa caataagtca
    17521 gaatactgat ccataaagcc atgagagcag ttggagtata ttttgttgta atttctatct
    17581 tattttctcc agcatttaca tccacattca ctaagccatg ttttttataa gagtcattta
    17641 cgactttacc gtttactttt acctgtgtac ctaaataagc atatactggt actgtcactt
    17701 tttgcttttt gttgcttgcc actgtgtaag taatgttatt atctgcagct ttatacttcc
    17761 ctttttccca cgattctcct acttttactt catgtttttc taagtgaggc ctttctttta
    17821 tcgcttctgt tggggcgtaa tcgtataatc ctcccatatc tgatgttgta taacgtggga
    17881 gtgtttcttc attgataaca atatgaccgt gttttgattg gataactttt gcgtaatttt
    17941 cttttgctgc tatagacgtg acgagtagaa acaccacagt agcgccaatg attaaccatg
    18001 aatatttttt attcatagtc tttttcataa aaacaagcgt tatgatcatt gaagaaaaaa
    18061 agatttggat acctaagata cgccaaggaa actggagtaa gcttatcggt gttttttgaa
    18121 acaaaaacca tggaaataaa ttagttacgc atacccatgt agaaactacg ccaacataga
    18181 tgattttttc ttttttctcc atttctttca gtatgaataa aggaaaaatc aacgcaacca
    18241 atcctaataa ccccatagtg tatgttgtaa tatcgttatt taacgcttga atgatactat
    18301 ctgaaataga aagtgcttct agctgcaaat ctgttttgaa gggactgtgt atttcttgat
    18361 aattcatctg ctgcaacata ggataccaaa aatagctagt taatgcacaa gtcaaaaaaa
    18421 cagcctttac taaattcacg accacaaatt ttatatcgtt ttttatttgc ttgcttaaaa
    18481 acaaacgaac tataatcata caagcaatca atgcactaca caatagtgta gttaagacat
    18541 gagtatacac aagtagactt atactgacag ataaagcgat gacttttttt tctctattga
    18601 atatcaatcc ataagcataa tataaaacag ctgggataat cgtaagagcg attgcttctg
    18661 caattgcgga tcgataatag atatcaactg ttcgataagt tgcaaaagta tataaacaac
    18721 taaataaaaa agcagcatct atctttttaa aaaacttttc tccataataa aaactaatca
    18781 gaatcgtaca aatatttaat aaccaaatat atagaacata acttaatatt aagttatttg
    18841 aaattccata aaaaacgact gcaggaatta ttgtaagaaa aggataaaaa tagttcagtc
    18901 catctccata attgttaaat gttgtatagt tgattggccc ttcaaaa
//
LOCUS       DACDTH010000057        18740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDTH010000057 DACDTH010000000
VERSION     DACDTH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.057"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            256..531
                     /locus_tag="IWK54_002078"
     CDS             256..531
                     /locus_tag="IWK54_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594192.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="IWK54_002079"
     CDS             544..2616
                     /locus_tag="IWK54_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9594193.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="IWK54_002080"
     CDS             2781..4667
                     /locus_tag="IWK54_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9594194.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="IWK54_002081"
     CDS             4679..5626
                     /locus_tag="IWK54_002081"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9594195.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="IWK54_002082"
     CDS             5646..6173
                     /locus_tag="IWK54_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9594196.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="IWK54_002083"
     CDS             complement(6236..6889)
                     /locus_tag="IWK54_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9594197.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="IWK54_002084"
     CDS             7021..7863
                     /locus_tag="IWK54_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9594198.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="IWK54_002085"
     CDS             8021..8917
                     /locus_tag="IWK54_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9594199.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="IWK54_002086"
     CDS             8920..9633
                     /locus_tag="IWK54_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9594200.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="IWK54_002087"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="IWK54_002087"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9594201.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="IWK54_002088"
     CDS             10168..10395
                     /locus_tag="IWK54_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9594202.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="IWK54_002089"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="IWK54_002089"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9594203.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="IWK54_002090"
     CDS             complement(11181..11723)
                     /locus_tag="IWK54_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9594204.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="IWK54_002091"
     CDS             complement(11960..12358)
                     /locus_tag="IWK54_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9594205.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="IWK54_002092"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="IWK54_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9594206.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="IWK54_002093"
     CDS             13471..14238
                     /locus_tag="IWK54_002093"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9594207.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="IWK54_002094"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="IWK54_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9594208.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="IWK54_002095"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="IWK54_002095"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9594209.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="IWK54_002096"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="IWK54_002096"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9594210.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DACDTH010000058        18057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDTH010000058 DACDTH010000000
VERSION     DACDTH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.058"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            316..675
                     /locus_tag="IWK54_002097"
                     /pseudo
     CDS             316..675
                     /locus_tag="IWK54_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(970..2103)
                     /locus_tag="IWK54_002098"
     CDS             complement(970..2103)
                     /locus_tag="IWK54_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9594211.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(2094..2762)
                     /locus_tag="IWK54_002099"
     CDS             complement(2094..2762)
                     /locus_tag="IWK54_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9594212.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="IWK54_002100"
     CDS             complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="IWK54_002100"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9594213.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="IWK54_002101"
     CDS             complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="IWK54_002101"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP9594214.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="IWK54_002102"
     CDS             complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="IWK54_002102"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP9594215.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="IWK54_002103"
     CDS             complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="IWK54_002103"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP9594216.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(6138..6911)
                     /locus_tag="IWK54_002104"
     CDS             complement(6138..6911)
                     /locus_tag="IWK54_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9594217.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            7076..7555
                     /locus_tag="IWK54_002105"
     CDS             7076..7555
                     /locus_tag="IWK54_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9594218.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            7612..7914
                     /locus_tag="IWK54_002106"
     CDS             7612..7914
                     /locus_tag="IWK54_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9594219.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7936..9393
                     /locus_tag="IWK54_002107"
     CDS             7936..9393
                     /locus_tag="IWK54_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP9594220.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9986..10738)
                     /locus_tag="IWK54_002108"
     CDS             complement(9986..10738)
                     /locus_tag="IWK54_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9594221.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10953..11189)
                     /locus_tag="IWK54_002109"
                     /pseudo
     CDS             complement(10953..11189)
                     /locus_tag="IWK54_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(11199..12157)
                     /locus_tag="IWK54_002110"
                     /pseudo
     CDS             complement(11199..12157)
                     /locus_tag="IWK54_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(12363..14045)
                     /locus_tag="IWK54_002111"
     CDS             complement(12363..14045)
                     /locus_tag="IWK54_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9594222.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(14056..14379)
                     /locus_tag="IWK54_002112"
     CDS             complement(14056..14379)
                     /locus_tag="IWK54_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9594223.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(14402..15808)
                     /gene="lacG"
                     /locus_tag="IWK54_002113"
     CDS             complement(14402..15808)
                     /gene="lacG"
                     /locus_tag="IWK54_002113"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9594224.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            16275..17282
                     /locus_tag="IWK54_002114"
     CDS             16275..17282
                     /locus_tag="IWK54_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9594225.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(17676..>18057)
                     /locus_tag="IWK54_002115"
     CDS             complement(17676..>18057)
                     /locus_tag="IWK54_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP9594226.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
BASE COUNT         5431 a         3811 c         2979 g         5836 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttggt cgtatcaaat gaaagcttat
      421 agaaacaaat tgaaggagta caaaatcttt caaagcatgt ctcggaaagg gaactgctta
      481 gataattcac ttatggagaa tttttttggc ttattaaaac aagagatttt tcacgggaaa
      541 gtttataact gtttcgagga attaaaatca gccatcgata gctatatcta ttactacaat
      601 aatgaacgga taaaacagaa attaaactgg caaagtcccg tccaatttcg taaaacaacc
      661 gttacggttg tgtaaaaata tcattttaga gcatgaaaaa aagagtggat ttctccactc
      721 caaaaaagtc taacttttgg gggtcactac atttttgatc ctttttattt gattaattat
      781 atccagctgg ataataattt tgggggcaac atttctatct tactattatc ttcctaaagt
      841 agtagacttt cttattggga aaaattacaa caaaaaggag tggcttagtt gacaactcct
      901 ttttgtttac aaatcaattg gatatctata aattgtattt tctaacgaat tttatagaca
      961 tctgatttgt caaattaagc tagacaatat gtctttatct atgcagctca aaaatacttc
     1021 caatggtgtt cgataattta atgattttct tggaatatgg tttcttttcg aggcaacgga
     1081 ttgaataaag gtttcatccg tgtccctaaa gtccattgtt ttaggtaagc catcctgctt
     1141 gagcccatgc actcggtact gatgagaaaa aattgatgcc tgtttctcct gaggaatgcc
     1201 aattccctga tcttttactt caataatcac gctacccttt tggtttgaaa tggtgacaat
     1261 taccagtttt ccttcaccat atttgattgc attgtcaact aaattcgaaa ccgcccgata
     1321 aatccaacga tctcgtccat gaatcagggt tggttgatct tcagtaaacg caaacaaaag
     1381 attcggataa ttcattttgt aggcatcaca aacttccgca cagatcagtg aaacgtctac
     1441 gacttccgtg ctaagatcct ctgagttatt ggataatgtc aaaatatcgt caatactgtc
     1501 ggtcatccga tctaaaagca ttaaatttcc ttgatcggca tgttgccgtt ctatttccag
     1561 attcgtacgc aaaatagcga tggcattttt ctgctcgtgg gtcagataac tatttaatcg
     1621 tcgataatcc tccaaatgac catcgaactt atcctttagc ttattaaaag ccgcatttaa
     1681 tgcctcgtcc ttcacagtat ttttaggaac ttgatcaata aacgttaaat cacgcgtaat
     1741 cgctaagatt tgattactgt aaattttgcg taaaaccaac caaagaataa acgttaataa
     1801 catgacggca gaacaaataa ccagcaccat cgctggtaaa tatttaaata gttcgtcctg
     1861 gtaagcttta tctatcatcg aaatagtcat aaattgattg ttggaagatt tcgaagcttc
     1921 catctgcgtc tctgcttgaa ccgtcttacc ctcttccata aaataaacat cttcacctaa
     1981 ggtagatgcg atactgattc cctccaaatt atttctcaga aagaagaaac tcactaaaat
     2041 cacgcctgtc accaacagtg aaaaagccag catcgttaac gtcattttaa aatttaatag
     2101 catagctggt acccctttgc tcgaatggtt tttaagacct ctttttcagc tgcttcagcc
     2161 agtttctttt ttagacgagc cagatgcact cgcagaaccg acgaaaaagg atcaaaatct
     2221 tcatcgtaga catgttcaga aatttcctca gacgaaacaa ccgctggatg acgctgagcg
     2281 atgcacagta agatatcaaa ttccttgggt ttcaggacca cctctgtctt ttgatagctg
     2341 acccttcgac ctaatggatc aatcttcaaa gcaccaattg aaatctgcgg cagcgcacgt
     2401 ccatgataac gacgaataac cgcgcgtatt cgtgctttta attctagtaa ctcaaagggt
     2461 ttgactaaat aatcatctgc tccaaaatcc aagccttttg ctcgttcttc aatctcatca
     2521 cgtgcagtaa taatgataac tggactttcg acttcttcag cacggagact ttgtaaaaca
     2581 gataatccat cttttttagg cagatttaaa tctagtagta tcccatcgta tgaattgatg
     2641 aaggccttct cttctcctga ctcgccatca taagctacat ctacagaaat attctcctgc
     2701 tctaggccct tttttacgga taaaaccagc tcacgattgt cctcaaccac cagtacacgc
     2761 atgtatattc actcctcata cggatttagt aattacactt atgcttttta gcatagtaca
     2821 cgctcttctt caaagtccac gttctttttg tgaagatttg aagaaattca tacattctgt
     2881 taaaaaacac ataattaaga atgattttac tgtttgaaaa tacctagaat agaaaaagac
     2941 tggaaccttt cctccagtct cactactttc tatttgcttg gttttcttgt atatactttt
     3001 caaagttcgt tggtttatga ccaataagcc tcgttagaat actgaaaatt gccatcaagt
     3061 cattaagtac cacatgtaag aaaatctctc taaagaaaaa ccccttcgtt ttaactcact
     3121 tactatttta gaattcgttc ccctaataat gcagcatgat tttacgcttt atgtataaaa
     3181 aaagactgca agcaattttg cttcacagtc tgatttctta tgatagtgag cttgttaaca
     3241 cttccaccgg tgtagcagtt tctttcaata cggcatttaa ttcttcaata tttttacgcc
     3301 cttgcgtatt catccactcc atcgctgctt cttttccatc aacgatatag ctttctacac
     3361 catctgccca agtcgcacgg ccgcaaagta caccattgaa ggttgagccg gcatttttcg
     3421 cgaagcgcag tgtttcttgg aacaactctg cttttacacc ggcgcttagg aaaataaatg
     3481 gcaggttagt ggcttcagcc tgttccttga agaagttagc cgcttgtgcc tttgtgtaca
     3541 cgacttcgtc tgtcccatag ccttccacat aattcatatt gacgggtact tccaccttta
     3601 acacatctac gccataacgt ggatcagaaa attccttcat catatcgttg actttatgag
     3661 gtttcttaat ggcgtattct ttcgaagtcg catcagagat gttcgcgtca taagaaacaa
     3721 gctccaagaa gaagggtaag ttttcagcct gacattctga gccgattcgt tcaataaagg
     3781 catgtttttg attattgatt tcttctgctt catcgacatc atagtaaagt aggaacttac
     3841 aagcatcggc gcctgcttct tttaaacgtt taactgacca gatcgataat aaatctggta
     3901 aacggccggg agttgacgca tcgtaaccag ttttttcata agctaacagc aagccggcat
     3961 tttttgctct aacgtcagct gctggcaagc catattcggg atctaataaa atcgatgagg
     4021 catatttcgt taattgtgaa gaaacaattt ctttaaattc aacgatttct gattcttctg
     4081 cttcgctgcc cttgaatttt gtgatcatct tcttcaaagc cccgcgttga tcaattgcta
     4141 aggccccaat cactccatct tcattgcata gttgcttgat atattgacgt ttttcttcgt
     4201 tcatttccgt atcctcctac actttctcca ctaagatttg atcatatagt ttttgataat
     4261 tggctaattg gacatagcca gtcgtcgctt cctgagcatt cagcattcct aaaacattac
     4321 cctgttttaa cacggcttca tccgaaagac cttgttgaat cgcagcggtc aatccggcaa
     4381 cggtcgcatc accagagcca actggattga cgacatcaat ctttggaatt gtcacgcaat
     4441 aaaaagtctc gtgatgtttg gcaaaggctc cgtcgccccc cattgaaacc actatccatt
     4501 caatttcatc aaacatcgaa tctgacaagg cggcttttaa atctgataat tctgttgaga
     4561 tttctcgtcc tagcagacca gccagctctt cggtattggg ttttattagc ttaggcttta
     4621 cactgcctcg caacacttcc cttaatgctg tacctgaaca atctaaaacg acaggctttg
     4681 gctgacaaac ttgcagcatt tgtcgataaa aatcagcagg caatccttct ggcaagcttc
     4741 ctgaaaaaga aatgatttcg gctttaacag ctaatttttc gaaatgttgc aagaacgccc
     4801 ttgcttcttg ttcagtaata acaggtccgt tttctaggat ttccgtctgt tttccgtcat
     4861 gtaaaacagc gatgcaatta cgtgtctctc cgctgatagg taaaaagtca tgagcaatct
     4921 cattttgagt caggtccttt ttaatctctt cacctaaaaa accgccgatt aaaccactag
     4981 caatcacctc cgtatctaat tgcttcaaga tacgtgtcac atttaggccc ttaccgccag
     5041 ctgttttgcg aacatttctt actcgattga ctgtgtcgag cttgaaggtt tctaatggat
     5101 aagaaatatc aatcgatgga ttcatcgtta ttgttaaaat catttgtcca cccgcttaat
     5161 cgtggtattc gccacgatcc catttctcga taaattcatc gaagaaatgc ggatcagact
     5221 gcttgtcact ggctttttcg acgtgatcga ttttggcgat caattttttg ttttcttcag
     5281 tttcttgata atcggcattg atgaaggcct ctaatatgtc acagatcaaa aattctcccg
     5341 taatccgagc gccaaagccg atcacattgg catttagttg ttccttcgca tagatagcag
     5401 tcgtcatatc gcgaaccagc gcactgcgga taccaggtac tttattgaca gcgttgttaa
     5461 tcccaacacc tgttccacac aaacaaactc ctagatctgc ttctccgcta gtcacggcct
     5521 ctccgacttt ttttccgtag atcggataat gggttctggt aaagtcatag gtccccatat
     5581 ccaacacttc atgtcctttt gattttaaga aatctgatac tgccattttt gtatctgtga
     5641 cgatgtggtc acagccaatt gcgattttca tttcattcat ccttcctaat tacgccattt
     5701 tatttaacat gtcgacacgt acttgatgac gaccaccatc ataatttgct tgtagaaatt
     5761 ccttaacaat attcaacgcc aactcatcgc caacaatttt tgatccaagt gtgatcatcc
     5821 gagaattatt atgtttacgg gtcatatacg cactgcgctc atccgaaact tctgctgcta
     5881 ccattccttt aacctttgtt gccgtgataa agctgccagc gccgtagcca tcaaccgcga
     5941 ttcctaaact accttcgttt tcattcagat catttgcgat cgccaaagtt gattccacaa
     6001 aatcttctga agccgtttca ctcttatcta ctactgtaat accttctttg atcaattttt
     6061 tcttgattaa ttcctttaat tccatgccgt cactgtctga accaattact acacgcattt
     6121 ttgtttcctc ctatttttta ctgaatgatt ttagtgaatt gctgataata actcttcagc
     6181 tcggctgaaa gagtagaatc cgtaaataat ttgtcgatct tgcgcaaatc atagaaatta
     6241 taaaaatcgc tatggtcgaa tttgtttgca tctgaaacca aacatttttc tgccgcatta
     6301 tctaaaacaa ttttttgcaa ggtaccttct tcaatactga aattcgaaac ctccgaaccc
     6361 ttcaatccat tcactccgac aaaagccttc ttaataccga ttttttgaac agcttcattg
     6421 gcaattgaac cgacaaaggc tccagtcttt tcccgatata gcccaccgat caaatacagt
     6481 tcaaatcttt ttatttcact taatagattg aaaaccggca gactattggt aacgattcgg
     6541 agagatgacg gcttgatgta tcgtgtcatt aattcgatcg ttgtacccga tccaagaaaa
     6601 atcgtgtcgt cttcttgatt gatatgagct gccgcatttt tagcgatgta ttccttctct
     6661 tttgtatgca gcttcttttt ttcagtatga gaaagctcat ttgcttctat agaagtcgac
     6721 atttccagat aatcgatttt ttgtgcaccg ccatgaacgc gtaaaagctt tttcttctgc
     6781 gcgagttcct ctagatctcg gcggatcgtc atatctgaaa cattcagtac ttcctgaata
     6841 aatttgactg ttatcgtccc atagcgatca cacatcgcta agatcgaacg ctgtcgttct
     6901 gttttcagca tattctgtac ctccacgacc ttataataaa acatttacga acatttttca
     6961 acacatattg ttcgtttatt ttcgtttatg cgttgacaat gattttgtaa gcggttatac
     7021 tcaacttgta aacatataaa taaacataaa cgaactaata aaggagctgt tttgtgtgaa
     7081 tgaattcttt ttcaaagaca ctgtctatgt ttctgacaag cagtcgcagg aagaagtgtt
     7141 cgaggaagtc tatcaagatc tgttaaagaa agaattggta accgaagatt ttttggagaa
     7201 ccttttagaa cgggagcgta attatccgac gggattagat ctctcacctg tctctaaaat
     7261 attgccagat atcgctatcc ctcataccga aagtgagttt gtgcgaacca cccgtatcat
     7321 tccaatcaaa gtaaaaaatg aaatcccttt ctacaacatg atctcgccaa gcgatcagat
     7381 ttccgcttca tttcttttca tgatcttgaa cgaaaatggt gaagcacagg ctggcatgtt
     7441 ggctaatatc atggatttca ttaattctgt tgacgaagaa aaactgtttt cgtttttcaa
     7501 attagaggac acagaagcga tttatcattt tttagaagaa catttttata tatagaagtc
     7561 tctgcctaac cgcaggataa aaaataaact tattttaagg ggagaattat tatgatcaaa
     7621 attttagcag catgtggagc aggcgttaat tcaagccatc aaatcaaaag cgctttggaa
     7681 actgaattga aaaatcgtgg ctatcaagtc acctgtgacg ccgttatgat caaagatatc
     7741 aatgaagaaa tgctgtctca ttatgacatt ttcgcacaaa ttgccaacac cgacttgggt
     7801 tttgacatta acatccctgt cgttgatgca ggtgctattc tttatcgtat tcctgctatg
     7861 gctgagcctg tttaccaaag tatggaaagc gcaatccaat cattaaacaa ataggactaa
     7921 agaaaggaaa agcatatgaa tacaattatt gatattgcca ataaagtatt taccccccta
     7981 attaatttag gtgcggcacc gttaatgacc atcgtattaa ctgttattgc tttatttttt
     8041 aaagtaaaac cgtcacgcgc tcttgaaggc gggctcaaat tagggatcgc cctgactggg
     8101 atcggagcta ttattggaat tttaactact gctttttcag acgctatgac agcttttgtt
     8161 gaacgcacag gtatttcatt aaacattaca gatgtcggtt gggccccact agcaaccatc
     8221 acatgggggt cgccatacac gctttatttt ttactaatta tggtgatcgt caatattgtc
     8281 atgttagcaa tgaaaaaaac caacacatta gatgtggata ttttcgatat ttggcattta
     8341 gcaattgttg gtttgttcgc gatcttctgc ggtgctaatc tgtttattgc ttctgcatta
     8401 gttattttca ttggtgtatt aaaaatttgg aactccgact taatgaaacc aaccttcaat
     8461 gacttgttaa atgcgccaga cagcaacccg atgaccacga ctcatatgaa ctacatgatg
     8521 aatccgatta ttatggtttt cgataaaatt ttcgacaaaa tcttcccttg gttggataaa
     8581 tatgatttcg atgctgccaa attgaatagt aaaattggct tctggggatc aaaattcgcg
     8641 attggaattt atttggggat ttttgttgga ttactagcag gacaaacacc aacgcaaatt
     8701 ttctcgctag cctttaccgc tgccgtctgt ttggaattgt tctctttgat aggcgcttgg
     8761 ttcatcgcag ccgtagaacc gttatcacaa gggattaccg attttgccaa taaacgacta
     8821 aaaggacgta ccatcaacat aggtttagac tggcctttca tagcaggccg cgctgaaatc
     8881 tgggctgccg ctaacgtatt agcaccaatc atgttgctag aagccatcat cttaccagga
     8941 aataaattat tgccactagg cggaatcatc gcaatgggtg ttaccccagc actattggtc
     9001 gtaactcgtg gaaaattaat ccgtatgatc gtaatcggcg cgatcgaatt accattattc
     9061 ttatggtccg gcaccttgat cgccccgttc gttactcaaa cagctaaagc agtcggagcc
     9121 tttccagccg gcttgaacgg gtccgccatg atctctcata caacgatgga aggcccgatt
     9181 gaaaaattcc taggttattt agtcggtaat gcttctcaag gacaaatcga atttgtgata
     9241 tacgctgcct tagcattagg tgcatacctt ttaatcttca tttggtacgc tcgtcaaatg
     9301 aaaaaacgca acgctgcata cgcagctgaa aaagaagaga atgcatctcc ggcaacggcg
     9361 cataatatga cttatgcagc cgaagctaaa taagctaaaa acaccccgaa tgtctttaga
     9421 cttcggggta ttattttacc tataaaaaat aaatagatgt aaaatccatt tcaaaaagca
     9481 tagcccatct cttgccacac ccaataaaga aattatcaac gtttatgctt tttttaactc
     9541 ctttaagctt gcctttgttg gtattcctca acgattcttt ttgtgttctt gtttgttttg
     9601 tgctttttga ctttttgttg tttaattgcg aattccttga tttctttttt cctgaatcgc
     9661 ttcgcatccc gcggttatgc gtgcgatttc atcattgctt tttctgatct aactcgtttc
     9721 atgtcaaaga gcttcagctg acttagtgtc catggttgtt ttgatatccc ttttcctcct
     9781 taataaagcg gtgattgata ctgctatttt ttccttcaca taaacataac atcccaaaaa
     9841 aatcgactat cttttttcaa atggtgtatt caagtattcg gaggaattag taatgatgca
     9901 ccctaaaaat aacataattc gcttaaattc aaggctacca aaaaactacc aagttctaat
     9961 tatctagagt ttggcagttt ctcatttaca caacatattt ttcttctttt tcgatttttt
    10021 catagtgctt ttgttcatca tcatccgtta taactactgt tacatcattc agattataat
    10081 atactgaaaa atctcttttc ccaattttcg aatgatcaat caataaaaag cgttcaacag
    10141 aattattcag agcgatctgt tgagtttggc cctcttcaaa agtcgaagtc ataattttct
    10201 gttcatttaa agcattacag ctgaaaaatg ctttatgaaa attcatatcc tgtagcgatt
    10261 tattcgtgat ttcaccaaca aagcattgag tcgtttcccg catctcgcca cctaatagat
    10321 agatttttcg attccctttt cgcttattta attcttcaaa aacaggcaaa cagtttgtaa
    10381 caatccgcag tgaatcccct tgaatgattt ttgacatgag ctcgattgta gttcctggtc
    10441 caagaaatat cgtatcacca tcattgatca tttcattcgc cttttccaca atccgctgct
    10501 tttcttcttg cagcataatc tgtttttctc tatgagaaag ctccacatgc cgataaaaat
    10561 ccttcaattt cgctccgcca tggacgcgtt ctaataatcc ttgtttctcc atttcgtcca
    10621 aatcacgtcg gactgtcata tccgaaaccg aaaacttctc cataatatcg ctgactcgta
    10681 ctgtaccgtt aacctctaat aacgacatga tcgcttggaa tcgatccgtt tttaacattt
    10741 catcgcctct tttcttttta agactatcaa attcttaaga aaagtgctag aacaagtttt
    10801 cttaagaagc cttctgcagg aaggaaagca aaatttttca gaatttgcaa gctgctattc
    10861 taaaaaataa aaccgaacga gcatgactgt catagtgaca gccttgttca ttcgactttt
    10921 atctttaata aatcatttta tttaatctga agttattgtg caagttcaaa aacaaagtag
    10981 ttaatgtctc ctcgatattt cgaaatcgaa tactcaacgg gcagacgttc tagcgaaaaa
    11041 ccctgtgtat ggaaatagaa cattggatct ccttcttcca catctaacaa atcgctgatc
    11101 gtatcatccg ctttgattac ttctagtttt ctggtgaccg ttttaattgg ataacccatt
    11161 tcttcaaaaa cttgatacat tttttctttt ctgatttgtc aaattaagct agacaatatg
    11221 tctttatcta tgcagctcaa aaatacttcc aatggtgttc gataatttaa tgattttctt
    11281 ggaatatggt ttcttttcga ggcaacggat tgaataaagg tttcatccat gtccctaaag
    11341 tccattgttt taggtaagcc atctcgtcta agcaagccgt ttgagtgctc atttagccct
    11401 ctttgcgaag gtgttcctgg atctgcgaaa aagatggcaa tatcgttcga gttacttata
    11461 gatcgccagt tggaaaattc ttttccacaa tcaaatgtca tggatttaaa caagtgttta
    11521 ggcatggatt tgaaccaatc atttaacctg ttttcgatat cttcggctcg tctgcctgtc
    11581 ggtttcaaag taataataac cttggataaa cactcaacca atgtaataac agcgctcttg
    11641 tgtttaactc ccacaatggt gtcgccttcg agatgaccaa attctttagt caggtgtgga
    11701 tagagactgt tccgttcatg aatcgttctg cgaaaggcct gttttccacg cttttcttga
    11761 tgattattcg gtttacgttt ccccttcata ggtaacgact gtgtgtcaaa aaggcctttt
    11821 ttaaacatcc gatagagtgt acgaacagaa caatcaattg gaaactcgcc acggccgata
    11881 atcacatcgg gtgaccaccc ttgagctacc ttttcttgaa catatgttga ttgttcatca
    11941 gataaaacgg ttgaacgtcg tccacaacga gatttatttt gtttgtattg acggtaataa
    12001 tctagcactt ttttccttgt ttgagaaacg aataaacatt ataaatggtt tgtctagagc
    12061 gatttaaact ttgtgcgaca agagcgacag gtttctcgat attgaaataa gactctatga
    12121 ttactagttc gtctgttgta agatgggtgt aggtcatttg tactcactcc ttattaattc
    12181 tttggtcgga actattttga gtgtagcaca aatgatcttt ttagttgtct agcttaattt
    12241 tacaatcgac gcctcaaaaa gaatacccga aatttaaaca gcaaaaaaag atagcgctta
    12301 ccattgtgtt taagataaaa cgctagctaa gttggctagc gctttattaa tctaagcaag
    12361 ctctaaaatt gcttcaatac aaaatcaact gctcctttag gatcacgtgt taaattgata
    12421 tattctgcac ctttagttgc aattactttt atccctaatt tgtctgcatc tttacggaga
    12481 tcatccaaat aggaattcac ttgaggtgct agaacgatta aatcaaaatt tttcatcata
    12541 tcataatgag aaccataagc tcctgcttta gcagaaagat taacacctgt ctcttttgcg
    12601 ccctcttcta atgcattagc caacattgca ctcgttccgg caccagcaca aagtactaaa
    12661 acgcgctttc cttcaccaac cgatgctttt gcttcatcaa ctataactgc cgcagttgca
    12721 ccaacttctt ggatttcttc gaccaattct tcttttactt cttctgcagc aatgtttttt
    12781 aactcttgtt ctactaattc agaatcatag gccttaacaa aaggtaaata aataatcgca
    12841 tcgacaacaa tcaatactac cgctaataca atcgccaaca cactaaaccc ggttcctaaa
    12901 accaagccaa tcggtgcggg cgtcgcccaa ggcaatacgg atataaagct attcatcccc
    12961 aagacatcga caaaaagttt gaatatccat acgttagcga ttggtgctaa taaaaatgga
    13021 attagaaagt acggattcaa aataatagga gtagcaaata gtaacggttc atttaccgca
    13081 aacgaaactg gaataaatga agctttacca actgctttca attgttttga tttagcgaac
    13141 aacatgaaca gaaaaggaac aactagtgtc gctccagtac cacccatcgt tccaacaaag
    13201 tttcctagac caactgttaa tacttttgag gcttgtgcac cgtttttaaa taattcaaag
    13261 ttagtttcta cgttcgcata aataattgca gcaatcgctg gttcaacaat cgatggtcca
    13321 tgaacaccaa caaaccagaa cattgccatg gctccccaaa tgattgcaat accgacatat
    13381 ccatcagcag cactaaataa tggctgcaat aattgaatta cacctgctgc aaatgatgtt
    13441 ccaaaaaatt gacgaactcc cgtatcaatc aaaacggcaa taaatactgc aattgaaaag
    13501 gggataatat ctctaaacat ctgtgtgatt gttccgggaa cttcttttgg catacggatc
    13561 gtcaaatctc ttttaacaca gaatttatac acatttacag taacaaacgc agaaacgaaa
    13621 gctgctaata atccctttgt ccctaaataa tcagtagcta tcccaccatc aatttgatca
    13681 gctgccatca ttacaaaacc acaaattgac gctaacataa cagaaacttc attaatctgc
    13741 ttacctgaat cagataattg acggttcatt gaattagcta aacatcgagc tgttgtagca
    13801 acaaccaaaa gaccaactaa tcccattgaa taaccataaa ctttccaaag ccacgtcgag
    13861 aatgcctctg gtaacgtcac gttaaatact tctggaacag ccgcaatcat aataaaaata
    13921 cttgaaaata gaatagctgg catagcagtt aaaaagccat cgcgaattgc ccctaaataa
    13981 atattacgtg agattttttc aaaaaacggt tttcccttct caatttgctt aataatcgta
    14041 ttcatttttc ttctcctatg ctttagcaaa tttttctttg tacaattcaa caaaatcatt
    14101 gataagttct cttaatagaa tcgttgtcat taaatggtct tgcccatgaa taaaaattac
    14161 gcctacttcc atttcgttcc ctgacgcttc ttcagctaaa catttcgttt gtgagttatg
    14221 cgctaaggtt aaattttcat cagcatccgc taataatcgt tcaacattat caaaattacc
    14281 ttctctcact tccttcaaca ctgttaataa tgaacttctg gcatctcctg cataagcaac
    14341 aatttcaaat cctaataatt gcaattcttt cctatccatt tccttgccct cctaactatt
    14401 tttatattat cgtttttgtt tcgctaacaa atttatacca atatgcagac tctttcggat
    14461 atcttttttg tgtatcgaaa tctacataga atagtccata acgcttagtg tatccattaa
    14521 cccaagagaa aacatccatt aatgaccaaa tgaaataccc ctttacatta actcccattt
    14581 tgatagcatc ggaaatcgca ttaaaatgct ggcgtaaata atcgattcga tcatcatcta
    14641 gaataatccc atcttcaaaa ttatccttat accctagccc gttttcagta atgtatattt
    14701 tattataatt tggatattcg tcttggattc gtacaagtaa atcatataac ccttttggat
    14761 aaatgatcca atcccaatcg gttttcggaa gatcctcacg cacgattctc tcgccaatcc
    14821 ctttaatgca atagacttgg ctaccttttt ctccggtagt gttgtagtga agtgtgcttt
    14881 ctcctttgta ctctttaacc caatggctac catagttatt gataccaaga aaatcatttc
    14941 gatttgctgc ttttttcatc tctctaaaat cattagaatc aatatctgca gattgcccat
    15001 ttgctgctaa aatctcatta atatgtgcca ttgtttcatc tgagtaatac cctaaataag
    15061 tagcatctaa taagaactta attgataacg catcatctaa tttcgcagca tgcttatctt
    15121 cttctttgtc acttgctgga tattttgtct ctagcgagtg aacaacacca atttcccctt
    15181 tatatccacc atctttaaaa atgtttactg tctttgcgtg cgcatacatc atgttgtgta
    15241 aacaagttac agtcttctct atatcaaatt gaacttctgg aggaaacgat ccgatcaaat
    15301 attgatttga agcaactgga taaatctcat taaatgtact ccaataatga acctcagaaa
    15361 attcatcgaa acagaactgt gcatagttta cgaaatgttc aacattttct cgatttaaaa
    15421 aatctccatt atcaaatagt tctttagggg tatcaaaatg atgaagagtt acaaaaggaa
    15481 taataccatg cttattcaat tcagcaaata ccttatgata atattccact cctgtttggt
    15541 tcacttcccc atagcctttt ggaaaaatac ggctccatgc aatagacatc ctaatactat
    15601 caattttaaa ttttttgcat aattcagcat caactggata ttgattataa aaatcactag
    15661 ctggatcagc agtatagcgt cccttttcta caaggaaagt atcccacgct acttgccctt
    15721 ttccaccttc ttttgtagag ccttctgctt gataagctgc cgtagctcct cccataataa
    15781 agtcttctgg taaagtttta taattcattt taatctctcc ttgaaaatta gtttgatcaa
    15841 tatgccaata atccataaat cacctcagca taattttaac atgtataagt aaattttttt
    15901 acacaacgaa aaaccgacct cgaatatcta ttttattgcg atatacaagc acttatatcc
    15961 cttgatgtaa aataaatata ccattcaaaa accgttttgt aaacgttttt ttgtaaaata
    16021 aatttatctt tttactaatt taatttacac gaaacaagaa aacggactga ttcccactaa
    16081 aatcaatccc acctactaat cattctccaa gtggctatcc attatcgatt caatcttctc
    16141 tgttaatgga ttaggctctc tgctgatatc agagtttcgt ttcctgctag aaaaagtaat
    16201 ttaagttact aaaagagcag tttcgattta ctttaacaaa gaaaaggatt attatatccc
    16261 taggaggatg aacaatggtt actattagag atattgcaaa aaaatctggt tactcaacgg
    16321 ctactatatc aagacttttt aaaggtgatg agtcactctc tattacatct gatactaaaa
    16381 acaaaataat cgttacagcc aaatctttag gatatgatct gacaaaaatt aagactactc
    16441 tgtacaagat tgccattctt ttttggatct cagaggaaca gttaaaaaag gatgtctact
    16501 atcaacatgt caaagatgat ttgaaaaatt atagtaaaaa agctaatcta gatttgattt
    16561 tcatctctaa aaacgatggt gtaaaaagca ttcctgacga tattactgga tttatcgcaa
    16621 taggacaatt gacaaaaaag gaattgtact acttaaatca aaaagggtat aagggggtaa
    16681 tccgaggaat aaacccatta cctgaacact tcgatactgt agaacctgat ttccacgaaa
    16741 tggccagaca agcaattaac tactttatta atgaaggttt cacaaaaatt ggatttattg
    16801 gaggaaattt ctttaaccta gaaacgggta aagaagagat tgatagccgt gaatatgcct
    16861 tccgatatca tttagaaaag agcggactac tcaatgaaaa gtatattttc tcccaaggat
    16921 catttactgt tgacgaaggg tatcaattaa ctcttaaaat gaaagctgag ttaaatgaag
    16981 atatgcccga ggcatgtttc ataccatctg acactatagc tgccggcgtt cttcagggct
    17041 gccatgaatt gggtattaac atccctaaag atatggcagt aataagtatt aatgatgacg
    17101 acatcgcaaa attcgttgca ccaccactta caacttttaa gatagatatc gaagagatag
    17161 ctaaaacctg tgtagattta ttaagtgatc aactggtata ttcgcgggac atatcaaaga
    17221 aaacattaat cggcgcaaaa ttgaccgtta ggaaaagttt tatccctaaa gaagaggtgt
    17281 aaaataagta attgaaactt atctttaaaa ctataattgt gtccacatag tagccgagca
    17341 atcgggttta tgcctattat gttttccata aataatttga tggtgatatt ttgacgtaaa
    17401 tacaatatga ttttttcatc gccaaattgt gcgcgctaat ttttgtttat cattcagaac
    17461 atttcctcct ttttatctaa gtatgaggtt ggataaacgc ttcatcttag tatcttgggg
    17521 gattttttat ctcgttggga atatatcaag catcagagcg cgcaaaaaga actggtcgaa
    17581 aaatcaatct accagttcaa tctcattcac tgtgatacta tgttttatac agataacgcc
    17641 atattataaa acaagttgta ttccgcaaat gggttctatt caactcttaa actatctata
    17701 aaagattcag ccaactccat tagcctctcg taatttagtt ggtgaacagc acttttttca
    17761 aaaaattttg aaccaattcc tacataagag gctccattgt tcaaatagtt ttgtgcatta
    17821 gtctgatcta cccctcctac agccatcaaa ggtaaacttc ctaaaggggc ttgcacttga
    17881 ttgaaatatt ccggtccaag acagttggcg gggaacacct taacaatatc agcacccatc
    17941 tcaaacattt tgaatatctc tgtgggcgac atggccccag gaattgttag aaggttctgt
    18001 tgcgaagttt gaaaatcaaa cgcgccctct ctgaactcca aatatcttag gctgtta
//
LOCUS       DACDTH010000059        18004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDTH010000059 DACDTH010000000
VERSION     DACDTH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.059"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(27..725)
                     /locus_tag="IWK54_002116"
     CDS             complement(27..725)
                     /locus_tag="IWK54_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9594227.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IWK54_002117"
     CDS             complement(718..2070)
                     /locus_tag="IWK54_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9594228.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IWK54_002118"
     CDS             complement(2293..3693)
                     /locus_tag="IWK54_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9594229.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="IWK54_002119"
     CDS             3854..4591
                     /locus_tag="IWK54_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9594230.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="IWK54_002120"
     CDS             4716..5195
                     /locus_tag="IWK54_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9594231.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="IWK54_002121"
     CDS             5192..5959
                     /locus_tag="IWK54_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594232.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="IWK54_002122"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="IWK54_002122"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9594233.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="IWK54_002123"
     CDS             complement(6862..7464)
                     /locus_tag="IWK54_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9594234.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="IWK54_002124"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="IWK54_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="IWK54_002125"
     CDS             8145..8729
                     /locus_tag="IWK54_002125"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9594235.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="IWK54_002126"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="IWK54_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9594236.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="IWK54_002127"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="IWK54_002127"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9594237.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="IWK54_002128"
     CDS             10630..10809
                     /locus_tag="IWK54_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594238.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="IWK54_002129"
     CDS             10831..11856
                     /locus_tag="IWK54_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9594239.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="IWK54_002130"
     CDS             12031..13275
                     /locus_tag="IWK54_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9594240.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="IWK54_002131"
     CDS             13428..14375
                     /locus_tag="IWK54_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9594241.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="IWK54_002132"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="IWK54_002132"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9594242.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="IWK54_002133"
     CDS             complement(15277..15519)
                     /locus_tag="IWK54_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594243.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="IWK54_002134"
     tRNA            15640..15712
                     /locus_tag="IWK54_002134"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="IWK54_002135"
     CDS             complement(15831..16469)
                     /locus_tag="IWK54_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9594244.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="IWK54_002136"
     CDS             complement(16592..17224)
                     /locus_tag="IWK54_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9594245.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17575..17874
                     /locus_tag="IWK54_002137"
     CDS             17575..17874
                     /locus_tag="IWK54_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9594246.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6165 a         3152 c         3558 g         5129 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    17401 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    17461 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    17521 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    17581 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    17641 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    17701 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    17761 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    17821 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    17881 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    17941 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    18001 gtat
//
LOCUS       DACDTH010000060        17150 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDTH010000060 DACDTH010000000
VERSION     DACDTH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.060"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            138..1475
                     /locus_tag="IWK54_002138"
     CDS             138..1475
                     /locus_tag="IWK54_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9594247.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="IWK54_002139"
     CDS             1523..3652
                     /locus_tag="IWK54_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9594248.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="IWK54_002140"
     CDS             3627..4316
                     /locus_tag="IWK54_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594249.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="IWK54_002141"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="IWK54_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="IWK54_002142"
     CDS             complement(5546..5740)
                     /locus_tag="IWK54_002142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594250.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="IWK54_002143"
     CDS             complement(5790..6230)
                     /locus_tag="IWK54_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9594251.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="IWK54_002144"
     CDS             complement(6234..7079)
                     /locus_tag="IWK54_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9594252.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="IWK54_002145"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="IWK54_002145"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9594253.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="IWK54_002146"
     CDS             complement(8703..9650)
                     /locus_tag="IWK54_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9594254.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="IWK54_002147"
     CDS             complement(9873..10223)
                     /locus_tag="IWK54_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9594255.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="IWK54_002148"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="IWK54_002148"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9594256.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="IWK54_002149"
     CDS             11646..11966
                     /locus_tag="IWK54_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9594257.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="IWK54_002150"
     CDS             11988..12452
                     /locus_tag="IWK54_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9594258.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="IWK54_002151"
     CDS             12657..13262
                     /locus_tag="IWK54_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9594259.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="IWK54_002152"
     CDS             complement(13301..14590)
                     /locus_tag="IWK54_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9594260.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="IWK54_002153"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="IWK54_002153"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9594261.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..16250
                     /locus_tag="IWK54_002154"
                     /pseudo
     CDS             15700..16250
                     /locus_tag="IWK54_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            16321..>17150
                     /locus_tag="IWK54_002155"
     CDS             16321..>17150
                     /locus_tag="IWK54_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP9594262.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT         5459 a         3105 c         3467 g         5119 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttgaagaga gtgtaaaata ttttgtgtaa atagaaaaaa
    16261 ggaagtccct tctgtagaat agagttacca caacacattc acagaaaaga ggacttccat
    16321 atgaacgatt ttactacaga aattatggaa atactaatca ataaaggtga tttggatgat
    16381 ttgtttcgtc gtcacctaga attggctatt aacgctttat tacaagctga attaacggta
    16441 tttcttgact atgaaaagta tgatagagct ggatttaatt caggtaattc ccgtaatggg
    16501 aattattcgc gctcttttaa aacagagtac ggtgaattga atttagtaat acctagagat
    16561 aggaatggag acttttctca acaaacttta cctgcttata aaagaaccaa tgactcttta
    16621 gaaaccacta ttattcagct agttcaaaaa ggaattacta tgtccgaaat ctctgatcta
    16681 atcgaaaaaa tgtatggtca ttattacaaa ccacaaacta tttcaaatat gagtaaaatt
    16741 gtgtctgaag atgtttcggc atttaaagaa agaactttag aagctaaata ctcagtcatt
    16801 tttatggatg ctactcatat tcccttaaaa agacaaaccg tatcaaaaga agctgtctat
    16861 atagtaattg gtatcaggtt ggatggaact aaagaggttc taggatttac tatagctcca
    16921 actgaatctg cttatgtttg gaaagagatt cttcaagatc taaaagaccg tggtttagaa
    16981 gaggttttgt tagtcgtaac agatggttta agtggtattg acgatagtat tcatagtgtt
    17041 tatccaaatg ctcaatttca acaatgttgt gtccatatat ctagaaatat cgctcataag
    17101 gttcgtgtta gtgatcgaca agaaatctgt agcgatttca aattagttta
//
LOCUS       DACDTH010000061        16935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDTH010000061 DACDTH010000000
VERSION     DACDTH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.061"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            297..1406
                     /locus_tag="IWK54_002156"
     CDS             297..1406
                     /locus_tag="IWK54_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9594263.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            1489..2385
                     /locus_tag="IWK54_002157"
                     /pseudo
     CDS             1489..2385
                     /locus_tag="IWK54_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            2401..3225
                     /locus_tag="IWK54_002158"
     CDS             2401..3225
                     /locus_tag="IWK54_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9594264.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            3257..4567
                     /locus_tag="IWK54_002159"
     CDS             3257..4567
                     /locus_tag="IWK54_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP9594265.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            4715..5974
                     /locus_tag="IWK54_002160"
     CDS             4715..5974
                     /locus_tag="IWK54_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9594266.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            5984..6856
                     /locus_tag="IWK54_002161"
     CDS             5984..6856
                     /locus_tag="IWK54_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9594267.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            6868..7698
                     /locus_tag="IWK54_002162"
     CDS             6868..7698
                     /locus_tag="IWK54_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9594268.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            7738..9921
                     /locus_tag="IWK54_002163"
     CDS             7738..9921
                     /locus_tag="IWK54_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9594269.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            9982..11202
                     /locus_tag="IWK54_002164"
     CDS             9982..11202
                     /locus_tag="IWK54_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP9594270.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            11199..12659
                     /gene="gtfA"
                     /locus_tag="IWK54_002165"
     CDS             11199..12659
                     /gene="gtfA"
                     /locus_tag="IWK54_002165"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP9594271.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            12736..13842
                     /locus_tag="IWK54_002166"
                     /pseudo
     CDS             12736..13842
                     /locus_tag="IWK54_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            14164..14373
                     /locus_tag="IWK54_002167"
     CDS             14164..14373
                     /locus_tag="IWK54_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9594272.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            14366..14515
                     /locus_tag="IWK54_002168"
     CDS             14366..14515
                     /locus_tag="IWK54_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP9594273.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            14550..15334
                     /locus_tag="IWK54_002169"
                     /pseudo
     CDS             14550..15334
                     /locus_tag="IWK54_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(15618..16703)
                     /gene="xerS"
                     /locus_tag="IWK54_002170"
     CDS             complement(15618..16703)
                     /gene="xerS"
                     /locus_tag="IWK54_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9594274.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT         5740 a         2554 c         3269 g         5372 t
ORIGIN      
        1 taaaaaagta aacatccaaa aaagggtgtt tacttttttg tttactaata tttttagtaa
       61 aaaatctcga caaaacatta agtaaacagt tatggtgttg ttaacaaaaa gaaaacgcat
      121 tcaaaacagg aggaattaag atgaaacatt tcaaaaaaat agctttaaca gggatagctg
      181 ttttaactgg gacattactc gcagcatgtg gtgggggaga tagtaattct aaagacgggc
      241 aagtaacttt aagctatgcg agttgagata ctgaccaagc tccaggacta agaaaaatgt
      301 tagatgaatt tgaaaaacaa aatcctgata tcaaagtaga aatggagaca acgccttggg
      361 atcaatattg ggttaaatta gaagctgcaa caacgggagg taatatgcca gatgtagtaa
      421 ccatgcactc tagcgaatcc tataaatata tgtcacagaa tgctttgatg ggacttgatg
      481 atgttatcaa agaaaatgat attgacatgg gaaactttac tgaaggaatt gctgatttct
      541 atacgcttga agacgaacta tacgcaatcc ctaaagatgc ttctgtagtg gggctatggt
      601 acaacaaaga attatttgat aatgcaggtg tcgattatcc agatgaaaca tggacatggg
      661 aaacactcag acaagctgca attgatttaa cagatgaatc aaaaggcatc tatggttttg
      721 catcacatat tggaacagag actggctact ggccatttat ttatcaaaat gggggagaag
      781 tatttgccga tgacaataca aaatcaggtt tgaatacccc agaagcacaa gaagctttag
      841 ctttttatcg agatattgca cttgttgatc atgcttcacc aacaattaca gaattacaag
      901 atcctgataa gttaagtcgt tttcaatcag gtagattagc aatgatgatt gaaggaaact
      961 ggtttgcacc gggatttttg aaaaatgact atctagttga aaatgttgct tctgccgtgt
     1021 tacctaaggg aaaagttaat gcaacaatta caaatggact tggttgggct gccagtgcga
     1081 gtacaaaata cccagaagaa gtgaaaaaac taatggcatt tttagctagt gaggacgcca
     1141 ataaaatcca agcggaaacg ggggcatcaa tcccagcact aaaaggcttt gaagacgagt
     1201 gggctgcttc ttcagataca attgatttga gtgcttttgg aaaaatgttg gagtatggag
     1261 tccctcgtcc atacaataaa gatggattac gagctgaaca agcagaagtt caaattatta
     1321 accgtattct gtctggtgaa gtaactgttg aagatggaac cgctcaaatt gaaaaagaag
     1381 tcaatgcgat attgagcgga aattaaatta gttaattata caatatacag gcgaaaggat
     1441 tctatttcgt ctgtatatta atggatgaat accaaaaagg gggatattat gaaaaatcga
     1501 atttttggaa aatatcgtaa agatcaagtg atatggggct atatttttat ttttccaacg
     1561 gcattaggat taattttatt aaatatatgg ccagcaattc aaacagcaat tttaagtttt
     1621 caaaaaacgg tagggttcgg tgatactgaa tgggtcggat taagaaatta ccaaaaaatg
     1681 tttgcagacg gtgaagtttt tcagtcttta ttaaacacat taatttatgc agtggtttcg
     1741 atacctagta ttgttgtatt atccttgttg gtggcagttt taatgaatag aaaagttaaa
     1801 ggattgtcga tttatcggac aatttatttt ctaccagtcg ttgctgcacc atccgcagtc
     1861 gcaatgattt ggcgctggat gttcaataat gattatggac tgattaataa catgttggct
     1921 aaaataggtc tcgatggacc agcatggtta acggatccat cgattgcgat ttattctatt
     1981 atcattgtag gagtttggag tgctattggt tataacatgg tgttattact agccggctta
     2041 taagaaattc ctaaagatta ttatgaagca gcggaaattg atggtgctag tccaatccag
     2101 cagtttttca acattacgtt accattagtt acaccaacat tattttttgt agttgtaaca
     2161 acagttatca atgctttcca agtatttgat gttattttta tgatgattac tcctaatagt
     2221 accgccatgt ttaaatcaca atcattagcc tatttattct ataaacattc atttatttta
     2281 aatgacaagg gatatggttc ggcaatcgtt atgttccttt tagtgattat attaattatt
     2341 actgcaattc aaatgaagtt gcagaaaaag tgggtaaact attaaggagg ctatcaaatt
     2401 atgaaaaata aatttttttc aaatgcgcca atccatatca tgcttatttt aggtggtatt
     2461 gcaatggcat taccttttat ttggatgatt ttaacttcag ggaaaacgtt ggttgaatct
     2521 acccaaattc cacctacaat tttccctgag aaatttcagc taagtaacta tcaacaagta
     2581 cttgatttat taccattcat ggctttttat atcaatacat ttttaatggt tgttggacga
     2641 gtgattggct cggttttatt ttcagcaatg gctgcatatg cttgtgcgcg tctaagtttt
     2701 ccagggaaaa atatcctttt cagcttagta cttattcaaa tgatgattcc cggttccatt
     2761 tttattactc cacagtattt attagcacaa aaattgggta ttttaaatac aattccagca
     2821 ttaatattac caggggtagt tagtgcgttt ggtacgtttt tattaagaca atttttctta
     2881 ggtttacctg atgaattgga ggaagcggcc tatttagaag gtgccaatat ttggacgatc
     2941 tttttcaaaa ttatgttgcc gcttgctaaa tcaggattgg ttgcacttgg aatattcaca
     3001 gcgttgtttg catttaaaga tttaatgtgg ccattgattg tcaatacttc aattgataag
     3061 atgcctttag ctccgggatt agcaaactta caaggacaat atgccacgaa cttcccacaa
     3121 ttaatggcag gttcgttatt agcaatttgg ccaatagttg tgatattcat cgttttccaa
     3181 agaaaattca ttgaaggaat tgctacaaca ggtagtaaat tataaaacta ttaattagtg
     3241 attggagagt aagtacatga caaaaattac atttattggt gcaggaagta ctatttttgc
     3301 aaaaaacgta ttaggcgatg cgatgttaac tccaagtcta caagatgcac atattgcatt
     3361 gtacgatatt gatgaaaaac gactaaaaga atcaaattta atgcttaaaa atattaataa
     3421 aaattcgaat gaaaatcgtg ctgatattgt ttcttattat gatcgcaaag aagcgttaag
     3481 agaggctgat tttgttatca atgctattca agtgggtggt tataaacctt caacagtgat
     3541 tgattttgat attcctaaaa aatatgggtt gcgacaaact attggtgaca ccatggggat
     3601 tggtgggatt tttagaaccc tacggacact accagtaatg ttagactttg ctaaagaaat
     3661 ggaagaagtt tgtccaaatg cttggttatt gaactataca aatccgatgg cgatgttaac
     3721 catgggtgtt ttaaaagcga cgagtattaa gactgttggt ttatgtcata gcgttcaagt
     3781 atgcgtacca gaattgtttg agcatttagg aatagccgat caatatgact taaatgactt
     3841 tcaatggaaa attgcaggca ttaatcatat ggcattcttg ttggaaatta caaaaaatgg
     3901 ggaagatttc tatccaacaa ttcgtcagtt agctgcagaa aaaccaaatc cacatccaga
     3961 ttctgtacgt tttgaattgt tgaaacgttt tggttactat attacagaat caagtgaaca
     4021 caatgcagaa taccatccat actttattaa atcaaaatac ccagaattga ttgacgaatt
     4081 agggattccg ttggatgaat acttacgtcg ttgtgaacaa caaattaatg attgggaaag
     4141 catgcgagag gacattgtaa ataatgggga gttaacacat acgagaagcc gtgaatatgc
     4201 ttcatatatc atggattcta ttacgacagg aactccgtca gtgattgcag gaaatgtctt
     4261 aaataaaggc ttaatcacaa acttaccgac agatgtttgt gtggaagtac cttgtttagt
     4321 cgataaaaat ggcgtgcaac caacctatgt cggtgaattg ccaacacaat tagctgcttt
     4381 aaatcggacg aatatcaatg ttcaagaact gacagtagaa gccgctttaa cactagataa
     4441 atcaaaaatt tatcaagcag cgtatttaga tccacatcta tcatctgaat tgtctttaac
     4501 agatatcaaa aatttagtgg atgatttaat cgaagcacat ggggactatt tacctgaata
     4561 taattaaaaa gaagctgaaa gagtaaggaa ttttcaattc cttactcttt tcttagttaa
     4621 atttagtaaa aatattgaca aaacacttag taaacagtta cattagtgtt aacaaaaaga
     4681 aaacgcattc aaattgtata aggaggagca taaaatgaag tggtctaaaa aaattatttt
     4741 agcttgtata gcactaggag caacgatctc tttgactgct tgtggaaaaa atcaggaaga
     4801 tgcaagcggt aaggtagaaa tagagttttt taatcaaaaa aaagaaatga ctcaaacgat
     4861 tcaggagatt gctaaagatt ttgaagcaaa aaaccctgat attcatgtaa aagttgtgga
     4921 tgtaccgaat gctggtgaag taattaaaac acgtatgctt gcaggcgatg taccagatgt
     4981 cattaatctt tatcctcaaa gtatagaatt gaaagaatgg gcgaaagctg gttatttaga
     5041 ggacttaacc gaagaaccgt atttagagaa tatcaaaaat ggatatgcac aacgttttgc
     5101 tattgaggat cgtgtatata gcataccatt aaccgctaat gtctatggtt tttactataa
     5161 caaaactgct tttgaggaaa tggggattca agcaccagaa acttgggcag aatttgaaaa
     5221 aatagtagct gagattaaag atcaaaacaa agtaccgttt gcgattgcag gagcagaagg
     5281 ctggacttta aatggttatc accaactagc tatagcgact gctgctggtg gtgaagagga
     5341 agcgaacaat gtttggcgtt tctcagaagt taatggaatt aatgctgaaa gcaaagaaat
     5401 gcaaaaagat tttaatcgat tagatttgtt aagagaacca aaagctttgc aaaacaactg
     5461 gcaaggtgcc ggttataacg atacagtagt tacttttaca aaaggtgatg cgttgattat
     5521 gccgaatggt tcatgggcaa tgccgatgat tcattcacaa aatccagatt ttgaagtagg
     5581 aacttttcct tttccagcag atgaagcagg tcaaagtcta acgattggcg caggagacct
     5641 agccttatca gtttctgcaa cttctcaaca taaagaagaa gccaaacgtt ttgtagaata
     5701 tatgacaaca cctgaagcga tgcagaaata ttatgatgta gatggtgcac cgtgtgctgt
     5761 tgagggcgtt atcgagaata ctcaggattc accattaagt ggtttaactg aacttgcttt
     5821 tactgatcgt catttagttt ggttagccaa agattggaat agtgaaaatg atttttatac
     5881 cttaacaaca aattatttac acaatggcaa tcaaaaagcc atgattaacg cattaaatgc
     5941 tttctttaat ccaatgaaag ctgacggaag gtaggaaaat aaaatgaaaa taaaagaaat
     6001 tttaaatcgt tactgggcaa tcatttttgt agcaattcca attattttgc agatgatttt
     6061 tttctacttt ccaatggttc aaggtgcttt ttacagttta actaattgga ctggtctgac
     6121 ttataatttt gattttgtgg gtttgaacaa ttataagatt cttttttctg atccgaagtt
     6181 tatcaaatcg attggcttca ctttaattgt gacgctttgc atgattgctg gacaaattgg
     6241 ttttggtatt ttgattgcgc gggcgttgaa cgcaaatatt aaagggaaaa cattctttag
     6301 agcatggttc ttttttccag cggtcttatc gggcgtggtt gtttctttag tatttaaaca
     6361 aatttttaac tatggtttac caaaaattgg tagtatgtta ggcattgaat ttttacaaac
     6421 cagtctttta ggaacaacat ggggagcgat ttttgcagta atttttgttt tactttggca
     6481 aggaattgcc atgccagtca tcattttctt agcgggtttt caaagtattc cacaagaaat
     6541 tattgaagct gctagaatgg atggggcgaa tgataagcaa attttttgga atattgaaat
     6601 cacctactta ttaccaagta tttcaatggt atttattatg gcattaaaag gtggcttaac
     6661 agcatttgat caaatttttg ctttaactgg tggtggacca agcgatgcca caacatcttt
     6721 ggggttactt gtttataatt atgcttttaa aaacaatcag tttggttatg cgaatgctat
     6781 tgctttagtc ttatttatct taattgggat tgtttcactg gcacaattaa aattatcgaa
     6841 aaaatttgaa atctaggagg tttcccaatg aaaacatcaa aagaaaaaaa tatttctaaa
     6901 tacttgcttt tattattagg gggcttcatg attttagttc ccttagtttc aacgctattc
     6961 ggttcattta aaacaacacc cgatattatg aataatttct ttcgttttcc taatccagcg
     7021 actttggaaa atttccgaag attgttagcg gatggaattg gtcattactt ttggaattcg
     7081 gcaatcatca caatcattgc agtagttgct gtgacagcaa ttattcctat ggctgcttat
     7141 tcactagcaa gaaaaatgac tcgtgggaaa gcatacggat tgatgtattt atttttaatt
     7201 ttaggaattt ttgttccttt ccaagtaatt atgattccga ttaccgttat gatgagtaaa
     7261 attggtttag caaatatgtg gggattaatc ttactctatc tcacttatgc cgtaccacag
     7321 acactatttt tatatgttgg ttttattaaa ctaaatgtgc ctatcagtct ggatgaggct
     7381 gctgaaattg atggggctag tcactttaca acttatttca aaattgtttt cccaatgtta
     7441 aaaccaatgc atgcgacgac tattattctt aatgctctat ggttttggaa tgatttcatg
     7501 ctaccattat taattttaaa tcgtgattct aatatgtgga cactaccatt gtttcaatat
     7561 aattatacgg gacaatattt taacgactat ggtccaagtt ttgcttccta tacagtagga
     7621 attgtcacga ttacactagt ttatcttgct ttccaaagaa gtattatagc tggaatgagc
     7681 aatggagcag tgaaataacc aataaattat tctcattata aaggagttca taaacttatg
     7741 attatatttg acaatcacac acaaacattt catttacaaa ataaaaaaat tagctatcta
     7801 ttaggaatcg aagaaaataa ttatttaacg catctttatt ttggaaaaaa gattacgcac
     7861 tattctggtg ggtatcgcta cccccgaaca gatcgttcct tctcacccaa caatgcaaat
     7921 gcgcaagatc gtttattatc tttagacaca ttaccattag aatttccagg atatggacat
     7981 ggtgatttta gagaaccggc gcataatatt aaattaggaa acggtagtcg tgtaaatgat
     8041 tttcgttatg atagctacga aattcaaaaa ggaaagccca gcttagcggg attgccagcc
     8101 ttatatacgg aagatgatga tgaagctgaa acgctgatta ttcaattgaa agacgcgatt
     8161 agtggattga ctttacagtt gttctacaca atttatcaaa atcacagtgt aattattcgt
     8221 tctacgcaat tagtaaataa tagtgatgaa acagtggaga ttaaccgctt agctagtcaa
     8281 tcaattgatt ttccaaatcg tccactcgag ttaattcatt taaatggtac ctgggcaaga
     8341 gaacgtcaaa tgactagaga aaaaattcat acagggataa aagtattaga tagtaaacgc
     8401 ggatcaagca gccatcaaca aaatccgttt gttgctttag tagatgccaa aacagatgaa
     8461 tttcaaggag aggcatatgg atttaattta gtttatagcg gtaatcacga aacggtgatt
     8521 caaaaagatc catttgacca aacacgggtc attatgggaa ttaattcatt taattttggt
     8581 tggcaactaa aaactggcga ctcatttcaa acgccagaag tcgtgatggt ttatagtaat
     8641 gaaggtctga atacgttatc acagacgtat catgaattgt ataatcaccg tttaattcgt
     8701 ggaaactatc gtttaaaaga acgtcccacg ttaatcaaca actgggaagc aacgtatttt
     8761 gattttaacg aagaaaaaat catgaatatt gtcgatgatg cgaagaactt gggcattgaa
     8821 atgtttgttt tagatgatgg ttggtttggc ggtcgtgaaa atgacttcac ttcattaggt
     8881 gattgggttg aaacagaagg caaacttgct tgtggtttgg aaaatttagc caagcaagtg
     8941 catgataacg ggatgaaatt tgggatttgg tttgaaccgg aaatgatttc tgaagatagt
     9001 gaactatttc gagcacatcc agattgggca ttgggtattc caggtcgtga ccgctcaatt
     9061 agtcgaaatc aatatgttct tgatttttca agacaagatg ttcgtgataa tatctatcaa
     9121 caaatgacgg cgatttttga tcgtgtaccg attgattatg tgaagtggga tatgaatcga
     9181 aatatgacag aagtctattc tgtcttgttg gactcaacga tgcaaggtga agtggctcat
     9241 cgttatatgt tagggttata tgaattttta gaaaagttaa caacgaatta tccaactatt
     9301 ttatttgaaa gttgttcggg tggaggcggt cgttttgatg caggtttctt gtattacatg
     9361 ccacaaactt ggacgagcga caataccgat gccgtggctc gtctgaaaat tcaatatgga
     9421 accagcttag tgtatccaat ttcaagtatg ggtgcgcatg tttcagcagt cccaaatcat
     9481 caaacaggaa gagaaacgag tttaactatt cgtggcaatg ctgcgatggc tggtgttttt
     9541 ggttatgagc tagatctaac gcaattaact tctgaagaaa aagcagaaat taaagaacaa
     9601 gttgcctttt ataaaaagca tcgccaactt ttgcaatatg gaacttttta tcgcttggaa
     9661 agtccgtttg aaggcaatga tactgcatgg atgtttgtct caaaagacca aacagaagcc
     9721 atggtctttt atttccgagt attagcagaa gctgcgcatc cattagtcac attgaaatta
     9781 gcaggattag cagaagaagc aacttatcaa atggaagatg taacatttgc tggcgatgaa
     9841 ttgatgaatc tcggttttta tattgactca gaactacatg gtgattatgc gacacaacgc
     9901 ttttatttga aacagatata gtagcttgaa tttacaatat aagtgcaaca ccaaaagaga
     9961 ctcattccaa acgaagaaaa tatgttttat ttacacgcat ttactaaaaa gcagcctgat
    10021 ttgaattggg aaaatgaaga agtacgtgaa gaaatctatg caatgattaa ctattggtta
    10081 gataaaggac taggcggatt tagaatcgat gcgatattaa acatcaaaaa aaaactagag
    10141 tatggcatct ttgaatctga tggagaagat gggttagcct tcattgggcc ctggattctt
    10201 aatcagtcgg gcattgaagt gtggttaaaa gaaatgagag agcggacatt taaaccacat
    10261 aatagtatga cggtagcaga agccgatgtt cctaatgaac gtttagatga atatattggt
    10321 gaagatggct attattcgat ggtttttgat tttagttata cggatattga tgtacctgaa
    10381 actggtgaat ggtttaaaga tagccaatgg acatggacgg atatgcgtac caatattttt
    10441 acgaatcaat tagttacgca agataaaggt tggggcgcct tatatttaga aaaccacgat
    10501 caaccacgtt caattaataa atatattcca gaagaatgga tcaatgatta tagtaaaaaa
    10561 atgttggcga cgctttttat gttactaaga ggtacccctt ttatttatca aggacaagaa
    10621 ctaggaatga ccaacataga aatggcatca ttagaagatt ttgatgatgt agctacgcat
    10681 tctcagtata aacgggcatt agaatatgga ttggcaccag agcaagcatt gaaagctgtc
    10741 aataaacgaa gtcgggataa ttctcgcaca cctatgcaat ggaatacgca aaaaaatgcc
    10801 agattctcaa cttctgataa tgtatggtta aaagtaaatc ctaattatcc agaactcaat
    10861 gcagaagctc agttaaccaa tcaacagtcg gtattcaatt attatcatca attaattaaa
    10921 ttaagaaaaa atagtgtata caaagacgtg ctaatctacg gacaatttct accagtgaaa
    10981 gataaaaatg aacatatttt gctttatgaa cgccaattag aagataaaat cgtattagtt
    11041 atcttaaacg ttacagcaac agaacaagaa tattatgtag atgaacagta ccaacaagta
    11101 ttaatcaata attatgaaga tgttttatta gatcaaagtc gaaaattgcg tttacgaccg
    11161 tttgaaagta ttgtattagc taattatgga ggacaattat gaaaattaaa aacgaagcaa
    11221 tgctcatcac ctatgcagat agtttaggaa aaaacatggg cgaattaaaa caagtcttag
    11281 acaaacattt acaaggtgtt attggtggtg tacatctgtt gccttttttt ccatcgacag
    11341 gtgatcgagg ctttgcgccg agtgattata cgacagttga tccagctctt ggaacatggg
    11401 aagatgtgga agcattaggt gaagattatt atttgatgtt tgattttatg attaatcaca
    11461 tttctcgtga atcaaaattt ttccaagatt tcaaaaaaaa ccatgagcag tcaccataca
    11521 aagaaatgtt tatccgtatt catgaatttt tcccagaaaa tcgaccaaca caagaagata
    11581 ttgatttaat ctacaagcgt aaagacaaag cgccgtttca aacggtagaa tttgcggatg
    11641 gtacgacaga agaggtttgg aatacctttg gggaagaaca aattgattta gatgtgacca
    11701 aagaagttgt gaaagaattt attcgagaaa ccatcaaaga tatggcgaac catggctgtt
    11761 ctttaattcg attagatgcg tttgcttatg cgattaaaaa attagatacc aatgatttct
    11821 tcgtggaacc agatatttgg gaacttttag acgaagtgcg tcaagaagca gcgaaatatg
    11881 acgtcgaatt attaccagaa atccatgagc attattccat tcaaatgaaa attgccaatc
    11941 atgattatta tatctatgac ttcgccttgc caatggtcac tctgtattca ctttatagtg
    12001 gaaaatcaga acgtttagcc aattggttga aaatgagtcc gatgaagcag tttacaacat
    12061 tggatacgca tgatggaatt ggtgttgtgg acgctcgtga tttattaaca gatgaagaat
    12121 tggattacac ctctgaggaa ttgtataaag tcggggcgaa tgtgaagaag gtctattcaa
    12181 gtgccaatta caataattta gatatttatc aaattaacag cacctattat agtgcattag
    12241 gcgataatga ccgtagctat ctgttagcac gtgcgattca agtatttgcg ccagggattc
    12301 cacaaattta ttacgttggg ttattagcag gaaaaaatga tattgaactg ttggaagcaa
    12361 cgaaagaagg gcgtaatatt aatcgtcatt actatgattt agccgaaatt gaagaagaag
    12421 tgcaacggcc agtgattcaa caattatttg cacttttaaa atttagaaat gaatctgcgg
    12481 cgtttgattt ggatggaacg attgaagtag caacgccatc agaatcagaa atagtgatta
    12541 cacgtaaaaa taaaaacggt agtgatagtg caacattaac ggctaacttg gatacgaaag
    12601 aatttatcat tgagtctaaa gatataaaaa ttttcactag tgttgcatta aaattttaac
    12661 ctattccata gaacaaaaaa tcctttatac tagcacttgg atgacttatc caagaccaat
    12721 aaaaaggact catacatgga taagtataaa acaaatacag catttaataa atggttttct
    12781 gccataaaat taggaaaact ttctgtcaac tcccaaaaat gtatttttga ttacaattcg
    12841 tatcgtaaaa aactgtcttt tgaagctgtg ctcaagctct ttctctacgc agttaatgaa
    12901 gaaaaagaaa gtttgcgtga tttgagtaca gctcttatcg accgatcgct ccaactagaa
    12961 accgatgtgt catcgattag tcatacccaa ttgtctcgag catttaatgc acttgaacct
    13021 aaagtgttag aagaaatttt tcaacaactg ttagaaaaag ttcaacatca aatcaaacca
    13081 accaagcgaa atcatctgta tctgattgat tccagtacgt tttcgcttag tttaaaacgt
    13141 cataagtggg cgactttccg tcaaacaaaa tcaggtgtca agttacactt aaactattgt
    13201 tatatggacg atactacgat gtatccaact gattttacct taacaaatgc ccatgaacat
    13261 gatctcaatc aactaccagt attggtgaat caaccagagg caacttacgt gtttgatcgt
    13321 gggtattagg actttgaaaa gatggatcag atgcattggg atggctattt cttcgtcact
    13381 cggattaaaa aaaacaccaa agttcacgtg atagatactc tggaaacatc gccagaaaca
    13441 gaaattctac gagacgagct ggttcgttta ggatctaaaa cctatctaac cgccaatttc
    13501 cgactagtca ctgttcaaga taagaacgga cgtgtgtttc aatttatcac caatcgaatg
    13561 gacgtctctt caaaagaaat ctctgatatg tatcacgctc gttggcaaat cgaattgttt
    13621 ttcaagcaca tcaaacaaca catgaccatt aaaaccttct tttctcaaag tgaaaaaggc
    13681 gtgcagaatc aattgatttt aacgatgatt tccgctctcc tcacgtttct catcaaattg
    13741 gagacgaaaa cggaaaaatc agtgttccaa atcaaacgat tctttcgtta tttactgttt
    13801 cagccctttg aatgttgtct agaaaaatta attcccacat gagaaaaaag aaatttggct
    13861 cgttactttt ggttttcagt tgaataacag ataaaaaatt ttctggaaaa agtcatcctc
    13921 tgcgtcagca cagttttttt tgacttattt tcagaggaaa tagttttcag aatattctaa
    13981 aatgttttat gcaacactag tgatattttt tagaattaga tttctaatgt gcattttttg
    14041 agtattaagg agcaagtata caaagattta taaagctgat taaatttgaa taaaaatatg
    14101 caggaacgct actattttta gtagtgttct tttttatatt tgaataaaaa atgtggtggt
    14161 taaatggtgg ttataacaaa aaatgaatta atgaaattag gttatgggaa gtatcagtct
    14221 gagagaatca tacgacaggc aaaagcaatt atggtttcaa aaagctatac ctattacgaa
    14281 aatagaagat taggactagt acctgttgaa tctgtagaag aagttttagg tatgaagata
    14341 gaggtagagg aggattttca gaatcatggt taattttaaa aaagataaaa atgggaaata
    14401 tcattttact gctagtttgg gattcgacaa acgaacaggg aaaagagtac aaaaaaacgt
    14461 agaggggtta aaacagttaa agaagaaaaa aagcgtatac cgaacttgtt tctaattatg
    14521 gaaaagaagc agaaacttct caaagtacta tgttgtttga ggagtttttt tataaattat
    14581 tcctacctta ttatcaaggg aaagtagaag aaagaacata taaaaatcgg ttatctacaa
    14641 tcaaaaaaac ttttcttatt tctttaaaat ggagttaaga gaaatacgtc caattgacat
    14701 tcaaaagtgg caaaataatt tattgagcaa atataaaaat tcttatgtaa gaaacatgta
    14761 tgggattttt cagatgtcat tagatcgagc agttgttttg ggactacttg aaaaaaatcc
    14821 tgcaaaagtt attggaaatg tgaagaaaag gaaaactaca gttgattttt ggacaaaaga
    14881 agaatttaca aaagttattt caacttttta tattgatgat ttgtaccaac attttggttt
    14941 tatttgtatg tggttacttt ttatgaccgt tatgcgtttg ggagaagcca ctgcattaac
    15001 ttggaaagat attgattttg aaaataaaaa cttgcgtgtg aataaaacac tctattataa
    15061 gagagccaaa acctatgagt ttgtagaacc cgaaacggct tctagtattc gtacaatcga
    15121 actagacgac aatacaatta cctttttaaa agcttggaag gagcgtcaag tttcaatagg
    15181 aggcatgtcg tttgtacttt cttataatgg aacacctact cagaaatata caatccgtca
    15241 tattatgaga agacatgcaa cgatcgttgg tgttcatata atccgcatac acggattaag
    15301 gcatagtcat gcttctttgc ttatatcaca ataaaaagat gcacttcgaa ttaaagaaag
    15361 gttaaaacat gctgatgtac aaacgacttt aggaacttat ggtcatttgt atctaaattc
    15421 attgagagag ataacaaatg atcccacaga ttatatccaa actaattttt ctaaaatcaa
    15481 accgacacct atttacgata ataaaaaata aatgataaac aaaagtagga tcacaccaaa
    15541 agaaacacac tagaaaaact ataatacagg cttttctagc gtgttgtttc ttatttccgc
    15601 ctatttatac attatactca taaagcattt aatgcatcga tcgtagccgc attgtctata
    15661 tgggtatata aagttgttgt attggtactt gaatgtccca attgttgggc agttaatacc
    15721 aatgaatttg tctgttggta gagtctagta gctaatgtat gacgcagttt gtgagggctg
    15781 acttgaattc taaagccttt tgaatatttt gctaccatcc gttcgattgc aataccggat
    15841 atacgcttgg cttctccttg gtaaattgct agaaataaag ctttttcgtt ttctgttact
    15901 ttatatcgtt ctttccgaat agctagataa ttagctaaat attccattgc gaatgctgct
    15961 atattgacag catctctttt atttcctttt ctagttacta ccacattatt cttatctaaa
    16021 gataggtcat ctaaattaat atttataacc tcggagagtc tcaatccaga tcctaaaatc
    16081 agtgcattga tcgccaggtc tctttctaca tctcgttgat aatagatcaa agctcgttta
    16141 ctcaaagtat ttttatattc attggcaata taatctagat aattttgtgt atccttatct
    16201 agaaataatt ttttctcaat ggcattggca cgtgcagcta acgtagtttt atctgtgatg
    16261 gttgctactt gatccatagg attattgtct aagtatggtt ttcctagagg attattggaa
    16321 cttttcgaaa gatatttaaa tagaaccttt aatgccgtga tatttctatt gatagtagaa
    16381 gtagatagac ctaagttatt attatctttg agcttagttt ttgttgtctc tttttttaca
    16441 ttttcttttg tgcgacgttt caaatgagat ttgtacaagt ttaggtcata ggcagttaac
    16501 tgtcttatcg aggtgataga agtatcagga acgatagact cactcagaag ccattcataa
    16561 aagagcttat aattttttag atattctaaa gtggttcgag gtgattgatc tttatcctca
    16621 cgatcttgaa caaaatcttt tatataatcg ggcatatgaa gtatgattgt atctattttt
    16681 tgatatattt ctgtattttt catgtcattc atcctttcaa taagtagggg agaagagtat
    16741 ctctataatt ggactatacc aattatagaa tgttttgtca attggtatgc tctaaaattt
    16801 attaatttgt ttcaaaaaaa gcatataaac aggtatacgt ttaataattt aaaataaatg
    16861 gtatacataa atagggaagg aaatagaagg gagcaatcta aattttttga attttcttaa
    16921 ttaattatat agaaa
//
LOCUS       DACDTH010000062        16386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDTH010000062 DACDTH010000000
VERSION     DACDTH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.062"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..280
                     /locus_tag="IWK54_002171"
     CDS             <1..280
                     /locus_tag="IWK54_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9594275.1"
                     /translation="WAYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEI
                     FHGKVYNCFEELKSAIDSYIYYYNNERIKQKLNWQSLVQFRKTTVTVV"
     gene            469..1137
                     /locus_tag="IWK54_002172"
     CDS             469..1137
                     /locus_tag="IWK54_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9594276.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1337..1723)
                     /locus_tag="IWK54_002173"
     CDS             complement(1337..1723)
                     /locus_tag="IWK54_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594277.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1695..3077)
                     /locus_tag="IWK54_002174"
     CDS             complement(1695..3077)
                     /locus_tag="IWK54_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9594278.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3233..4420)
                     /locus_tag="IWK54_002175"
     CDS             complement(3233..4420)
                     /locus_tag="IWK54_002175"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9594279.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4427..5458)
                     /locus_tag="IWK54_002176"
     CDS             complement(4427..5458)
                     /locus_tag="IWK54_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9594280.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5474..6928)
                     /locus_tag="IWK54_002177"
     CDS             complement(5474..6928)
                     /locus_tag="IWK54_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9594281.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            7103..8008
                     /locus_tag="IWK54_002178"
     CDS             7103..8008
                     /locus_tag="IWK54_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9594282.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8099..8776
                     /locus_tag="IWK54_002179"
     CDS             8099..8776
                     /locus_tag="IWK54_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9594283.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9057..9953
                     /locus_tag="IWK54_002180"
     CDS             9057..9953
                     /locus_tag="IWK54_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9594284.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10008..11378)
                     /locus_tag="IWK54_002181"
     CDS             complement(10008..11378)
                     /locus_tag="IWK54_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9594285.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11616..12362
                     /locus_tag="IWK54_002182"
     CDS             11616..12362
                     /locus_tag="IWK54_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9594286.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12359..13198
                     /locus_tag="IWK54_002183"
     CDS             12359..13198
                     /locus_tag="IWK54_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9594287.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13199..14149
                     /locus_tag="IWK54_002184"
     CDS             13199..14149
                     /locus_tag="IWK54_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9594288.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14290..15042
                     /locus_tag="IWK54_002185"
     CDS             14290..15042
                     /locus_tag="IWK54_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594289.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15061..16212
                     /gene="queG"
                     /locus_tag="IWK54_002186"
     CDS             15061..16212
                     /gene="queG"
                     /locus_tag="IWK54_002186"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9594290.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5329 a         3048 c         3286 g         4723 t
ORIGIN      
        1 ttgggcgtat caaatgaaag cttatagaaa caaattgaag gagtacaaaa tctttcaaag
       61 catgtctcgg aaagggaact gcttagataa ttcacttatg gagaattttt ttggcttatt
      121 aaaacaagag atttttcacg ggaaagttta taactgtttc gaggaattaa aatcagccat
      181 cgatagctat atctattact acaataatga acggataaaa cagaaattaa actggcaaag
      241 tctcgtccaa tttcgtaaaa caaccgtaac ggttgtgtaa aaatatcatt ttagagcata
      301 aaaaaaagga gtggatttct ccactccaaa aaagtctaac ttttgggggt cactacagtg
      361 tcacagtcct ttttttagta aaaggataca tttcttcctt gtatattcca tgcgtgttcg
      421 tgagaaagca tatataattt aattaacaga tagttagggg gagaaaaaat gattttttca
      481 attacaatga aactggtgtt cgggttgatc ggtttattat tagtggtacg tttgcttggg
      541 aagaaatcca tgtcagaaat cacaccgttt gatttagtct atacgcttgt tttaggtggg
      601 atcttagaag aatcaaccta tgatgacaat gtccatgtag ggcatgtcct atttgccatt
      661 gcgctttggg cagtaatgat ttatggtatc gaacgtatcg tccaaaaaaa tgaaaaggtc
      721 aatcgttggg tcaaaggcga gccatctgtt ctaatcaaag acggcgtcat caacatgaca
      781 gagttgacaa ataaccatat cgagatggag cagttgcgag caatcttaag acagcaagaa
      841 tgcttttctc ttgaaaatgc caaacatgtt atcctagaga atgctggtca aatgagtgta
      901 ttgaaaaaat cagatgaaga caaagcttta tcgatcctgc ttgtagatga agggcaaatt
      961 cagcataaag tgcttcagtc caatcaattg actgaagcat ggcttatgga gaacttgaag
     1021 aaagaaggct atgcagatgt aaaacagctg atttatgttg aatggtcaga agaaaaaggg
     1081 ctctacatac taaataaaga agacgtcatg catgaatctt accgaataga tggatagata
     1141 gacagaggtt gtgaaatcag cgccttggct tgttactcaa aactaagcga ctttttcaca
     1201 acctctgata tctaatatac gttgttccag aagttgcttc ttcgaaaaat aagccaaaat
     1261 tccacaaaaa tttaagaagc aattttcgaa aaatcctttc ttattttttg aagttgatca
     1321 cttctgtcac aacctctcat gtcacaaacc atttttttgt ttcctttaac tcaatatacc
     1381 gtacgtcttc ccacaatatc ggctgatggt caatcataaa gtaatcttca taggcttcac
     1441 caatgaagtg cccttcgacc tcatccaaga gtcttccaaa ttcatctttt aaattgagct
     1501 gaatgattac tgctttacga gataaaaagg cttctgtcag tatactatcg atttcttgca
     1561 atgtcatttg ttgtttagga agaggctgtt tgcgtgcttc ttcttcattt tttttgatac
     1621 cgttgactag ttcttccatc gcaaaagcag tcccccattt taatccaaat ggccgatctc
     1681 gatactcgtt atagtcaatg aatttttttg ctgtccgtct catcttttcc accttctaat
     1741 ccactcatac cacctgcatg cccgccaacg agagaactac gtgcgatggc gcgtcctcct
     1801 tgcaagagac tatgcgcatg gatgattgat tgaaagccgt atttttttct aatcgtatcg
     1861 acaacaaaat caactttcgc ttcatttatc tgcttttcag gatcttcaaa aaggttaagc
     1921 tgtaaacctg tcgcgtatac gaggtcggaa cagttcacgc ctacacgccg aatatcctgg
     1981 tagtggtaat gctggtggaa aatggtcaat aaagcatctg ctagatctct gctagcattt
     2041 gtcgcttcga ttttcatttg ctggtgaaac ccggtcttgc catttggatc aagatagcct
     2101 aaagagtatc ctacccataa gctgactact tgtgtttttg ctgcagatcg tctcaagcga
     2161 gtgccgactt ggtcggccat ttctttgata acgatctcga tttcttctcg tcttgtataa
     2221 tctctatcta ggatttgact atttccgata ctttttgatt ttggtgtata ttcttgtccc
     2281 aaaaaacttc gatcgatgcc ccaagcatgg gcataaagct gagtaccgat gacccctagc
     2341 ttgcttttca agtgataata atccgcgtga gctaaatcat aaacagaatg tatacccatt
     2401 tgtttcagac gttttgctgt tcgtttgccg attccccaaa agtcagtgat atctgagatt
     2461 ttccataact tttctggtac atcttcataa cgccattcag caaacatttg ctttccgtct
     2521 tttgagccat tgtctaaggc tagtttagca agtagcgggt tatcgccaat tcctatagtc
     2581 gtgtagattc cggtctgctc aaacacatct ttatggatca gtttagccat gtcgtacgca
     2641 tttgttttac caaagagtgc catgctattt gttacatcaa ggaagctttc atcgacggaa
     2701 tagacatgat ggttcgcttc gtctgcatat tttttataga tagaattgat ttctctgttc
     2761 ttttccatat aataggccat ccttggggca acaatatata aatcacttgg ataggggaag
     2821 ggaagatctc tggcacggct gatattggtg atgccgtaag ctttttttgc tgcaggactg
     2881 gaagccaaga ttaatccgct accccgtgtt tctgtcgaat cggacggata ggacatgact
     2941 actaatttag ttgtaagggg atcaagatcc ttttcaacgc actctacgga ggcgtaaaaa
     3001 gatttgcagt caatacataa aatatctctt gaaggttctt tttcataaac aaataccgga
     3061 ttttgattcg cccacataac aatcacctct tcaacacaat tagaacacac gttctctttc
     3121 cgtgtcaata aaaaacagca attttcaaat acgagcaaaa ggctcacatt tggaaattgc
     3181 tgagaggtta tttttatcta tttgttttta gctgagatcg ttgatttttt tatcaaacat
     3241 gaacagcttc tggatcgatc tttgttgtcc catttccaat gtgtgcaaca taaaattccg
     3301 gagcatagcc gacaactttc atgtattcat catagacatt gtttttgaag gcaggtatat
     3361 tttcttcttt gacaagagca atcgcacagc caccaaatcc agctcctgtc attcgtgcac
     3421 ctaagacacc ttcttgtttt tgagcagcat ctactagtgt atctaattcg atcccagtta
     3481 cttcataatc atagcgaaga gagtggtgag aatcatttaa cagttgacca aatgcttcta
     3541 agttcccagc ttctaattca gcttttgcct tcaaagtccg ctgattttca gaaacggcat
     3601 ggcgagctct gcgtatcaat gtatcatcgc cgatttggtc aatagttgat tcaaattctt
     3661 cttcatctaa gtctcctaaa gaagagatag gcaaagtttt ttgcaaacgt gccaaggctt
     3721 cttcgcattc gctacggcgt tcattgtatt ttgaatctgc caattcccgc cgcttattcg
     3781 tattcatgat gacgattgca taaccatcta gtttcactgg aaccatttcg tatttcagcg
     3841 tatttgtatc taataagatt gcatgatcta gctcaccgaa gccgatggca aactgatcca
     3901 tgatacctga attgacaccg atgaattcat tttctacttt tttacctgtt tggactagtt
     3961 ccaagcgatc tgtttttaaa tcaaacagat cttctaatac aacacctact aataattcta
     4021 atgaagcgct actggaaagt cctgcaccat tagggattgt tccttccact aacaactcaa
     4081 acccatgatc gattgagtgt ccagcttctt ttaatttaag gaccattcct tttacgtaat
     4141 ttgcccagtt tgctttttta tcatattcta gctcatctaa agtaaaacta atgacaccga
     4201 tatcgtcaaa gtttgtggaa taaactaata ttttattatc gtctcgaggt gctgctacac
     4261 cttgcgttcc ataagtgatg gaagcgggga agacatggcc tccattgtaa tctgtgtgtt
     4321 caccaatcaa attgatgcgt cctggtgaga agtattgtgt agttttttta ggtccgaatg
     4381 tttcggcaaa ttttgtgttt aagcttcttt tgatttccat gatgagttat tcctttcttg
     4441 ttttgatttt gaattctgtc actgtttcaa atactgatcc agcttttaaa tgaatcgaac
     4501 caaatgctga gaagcgctct gcacctggag ctgtttgagt ctcaaatgtg atgccaccgt
     4561 gatgtgccag ttttcggttg cgcatttctg gtgtttctgt gccgaaattt gcagtgaaaa
     4621 taaccacact agaagcatct gtagcaatac ctacttggat tcttttatcg gggctagtca
     4681 agcgagcggc ttctttaccc aagccggttt cttttaagaa aaatggatga tcgattccgt
     4741 caacaagatt tttttgatcc aaatcactag caaaaacgga cgctaatttt ttaggtattt
     4801 gaaaatcgaa agcacttcct gcagcatttt cttttacccc tataggaata ctatctgttc
     4861 gcaaaggagc ataagcttcg ctattgagcc aaagctcatg ttgatcgatt gactgagaag
     4921 catctcccgt caagttgaag taaacatgat ttgtcggatt gaataaagta tcttggtcac
     4981 tggtgccgcg agaggtcact cgccagatat tatcttttgt caaagtatag cggatttcca
     5041 catccattgt acctggaaaa ccatgttctc cgtcgggact tgtagtagta aaaatgacag
     5101 aagcttcatt ttcgccattc aaaatgacat aagaccattt ttttaattca aatccaggag
     5161 caccaccatg cagattatgt ccagtttttg gatctgtggc taattggtaa gtttttccat
     5221 tgagagaaaa gcggccattt tcgattctcc ctgcggtgcg accgatagaa gcaccgatat
     5281 acggatcttt ttcgatatat tcttcagcgg tgtcaaatcc caaaactaat tctcgatcat
     5341 ttgattttaa actgacgatt ctagcaccta aatcagagat agaaagtgct agcttgtttt
     5401 tgttctccaa ggttatcaag tgataacctt ggccaaaatc tttttctgta atcttcattt
     5461 tcaagatcct ttcttaatgg gaaggaacta ctgcatcgtg aagttcatct tccattgtat
     5521 cagctggagc caatttgttt tccaattcct caacgatttg tgcatgtttt ttctctgtca
     5581 atgtaatctt ccaagcaaaa ataatagcgg agaaaatcat caaagcagca ggagcataga
     5641 atgcataagc tttgaaagtc gttacgccat gagcagtgat atcagcagca gtagcattac
     5701 cagtcatccc agaagcgata gcgacaaacc ctacgatccc gttagacaaa gcaccagcga
     5761 ttttatctag taaagggcga attgatagag taacagcttc gttacggata ccatttttcc
     5821 attgaccata ttcaacagag tctgtgatcg tcatcaaagc ggataagaaa atcaattgtt
     5881 gagggaagta gaacaatact agcccgatga taacgattgg caaacttgaa cctgcgatcg
     5941 taaagaaaaa gtaagaagaa atcatcattg cgatccctcc aaggtaaacg tagcgacgag
     6001 agatgacgcg tgtaagcatt gggaatagtg gaacagctac taaaccaagt aaggcagcaa
     6061 ctagaccaac gatccaaaat tcagttggtt ggccaagtac atatttgaag aagtagaata
     6121 atacggcagt tgttgctaca ttggcaatcg cataagcgat ataagcaagg gcaagccaca
     6181 tcaactgatc attttgagca actgctttga acacatcttt gatcccagct ttttcatttt
     6241 gctgacggat cgcagtatcg acttcttttg tgcccaaggc agtgataatc gcagtaccgc
     6301 ctgaaataac agcagcaatc acaccgaacc agaaccagcc tgaagcgcct tcaatatgtt
     6361 tatgagtaac aagataggta aagaaagtaa cgatagggac aacgactaga gtagtaccat
     6421 ttgcacctag agaagagccg aaacgagcaa acgtagcaaa tttttcacgt tcctttgttt
     6481 ccgtactcaa agctggaatc attgaccaaa aagcgatatc cttaaatgaa taaaaactat
     6541 ccaataagat gaaaacgatt gcaaataata tgataaataa agtcgtatta tgagtagcta
     6601 agccaaagaa attggtaaag attgctacca agagtacgga actaacaacg ccgccgataa
     6661 ctaaccatgg tttgaattta ccgtattttg tacgagtatt atcgatgatg ccaccaatca
     6721 aaggatcaaa aataatttca actaagcgga tacctacaac taagcttgta actgtagcaa
     6781 tcatttttgc attcgttgcc ttatctgttc ctgcgaacat ctggcttgtc acgaatacca
     6841 taaaataagt gcttaatgta acatagaatg tgtcgtgtcc aaaagcgcct aaagcgtagg
     6901 acacacgttg gatcattttg cttttcataa tctgtattcc tctcctgttc aatatataat
     6961 actaagtaaa ccggttacaa cgaataacaa agatctttta atgacttaaa cttaacaaga
     7021 aatcgcttcc tttttaatat atttctgtga cttttattta actttttgtt gcattatact
     7081 aaaggagtaa ggagggatag acgtggagca atcgattttt ttaaaaaagg aacatcaaca
     7141 agtagaaaaa gaattgtatt ttgatttttg tggtttctct aaaacgatgc cgtttcattc
     7201 ttttggtcct gctattcgca atgattacat cctgcatgtc gtgatggaag gcaaaggatc
     7261 ttatcatgtg aaaaatcaac aattccaatt gaaaaaaggt gatctgttct tgattcgtcc
     7321 aggagactcg acgttttact tatcagatgg agaagatccg tggatgtatt gctggctttc
     7381 atttggtggg agtgctgcag aagaaatcat tcggcagtcg cttttcagag aagaccaata
     7441 cacgatggtc tcttctgaca tacaaaaata tatcgacatt attttagaat gcatgaaaga
     7501 tacggaagaa accttggaaa atgaattaca gttaacagca ttaacctacc gttttttatc
     7561 agtcctttta aaagatggcg gaaaggtcag cggtggtgta caaaaggttc attcctcttt
     7621 agctgttgaa acagtcaaat acatcgaggc acattatgct gaaaaattgt ctgttgagga
     7681 aatcgcccag catttagcgg tcaatcggag ccatttgtcg cgagtattca aaaatcatat
     7741 gggtaccagt atcaaggaat atttgatcgg ggtacggatc aatcgagggg cgtttcttct
     7801 ttctctgacg gatgaatccg tggaaagtat tgcctatcaa gtagggttca atagtctagt
     7861 cgtatttagc cgaatgttca aaaagagtac cggagaaacg gctacgaact atcgtaaacg
     7921 tatgaaaaac gaaaatttta agaatattgc tttggatacc ttgaaaaaac agttggaaaa
     7981 acaaaccgca gtttcttgga caacctgatt agtctggtta aaatgacaag catcggcaaa
     8041 agaaacttgt ggcggttatg ctacactgta aagcgaagac taaaaaggag gaaaagggat
     8101 gcgtacactt atcatcatta gccatccaac gatcgaagaa tctttaaatc agcaattttt
     8161 caaagaagct tctgctggtt taccagctac ttggcatcat ttggaatcaa gttatccaga
     8221 cggaaaaata gatatagaga ctgaactaca gctcttggcc cagcatgatc ggatcatttt
     8281 ccagtttccg ttttactggt atagttctcc tgctcattta aaaatttggc aagataatgt
     8341 cttggaacga gcagcacatg tattaaaagg gaaagaacta ggaatcgtat tgacaacagg
     8401 tgtggcggaa aaagaatacc aagctggagg aaaagaagaa tatacgattt ccgaattttt
     8461 gaggccgtat cagcgaatcg caaataaatt ccatatgacc tatcttccgc cattcgtttt
     8521 agcacaattt atgtatttat cccaggaaaa acgttgggag aagctaattg cttatcagca
     8581 gtatctctca ttggaaggca aaccatcgct gactcagcgg attgactggt ttattcaaag
     8641 agtacaggaa aatcagaaaa tgcaggaaga agaatctgaa aaacaagcat atatcgtaga
     8701 agcactgact gaggcaaaag agcagatcga agacttgacc tttacactgc aagaaatgaa
     8761 aggaacttca ttatgacaaa tgattggctg atccaacaat tagatgaatt aagcgaaaaa
     8821 tcagaatatc aagaccgtgc gctcttatat gaaataaaga aactgatggc tgagatagac
     8881 gaacgaacaa tgcagctgca aggagaaatc gacggacaat cttggaatca tctcaactgg
     8941 tgaaattttc ttataaataa caaagataaa aatgtgcaca gcgacaatta tttgctacaa
     9001 taagaatatg aagcgtttga taacgctatt atacataaaa attggtggtg aaaaaaatgt
     9061 atgatgaaaa tgagtgggga gaaaatgttc ggatgacccg aagaagacag caaaaacagg
     9121 cagctcgtaa gcggatgatc atgattgggg cagcagttgc cgtgtttttg atcatagcag
     9181 gaggagttgc gggtctatat tttcatggaa aaaattcgtc agaagcgaaa agtacgacca
     9241 taacggaaac aacgacaaag aaaacagaga cgaaacaaac aagtgagtca tcaactaaaa
     9301 caaaagagac acaaacttct gaagaaaaaa cagcaaatgg tttgccagag caacttcaag
     9361 caatcgtaaa tgattattcg aaaaagatcg aagaaaagac gcctgcattg attgaagaat
     9421 accagacaga aattcaggga aatcaagagg gaatagctgg tttatcagca gttgctaacc
     9481 aaaaagcacg tgagcttcaa gcaatctccg acgaagggat cagaaaatta cgtgcagcgt
     9541 atcaggcagc agaaaataaa gacggtgtag acttagatac tttgatcaat caattatcgg
     9601 ctaattatac gaatcatgtc gctaagattt ccgatattta tttgcagact agcgcctctc
     9661 ttcaagcaga gtcaacaagc acacaagaca caacatcttc tgattctgag acagcagagt
     9721 cggtagaagc ttctgaaatg caagctcgaa caacacaaga cacaacagat tcctctgaaa
     9781 ctaataccaa cagcgcgact tcacaggctg cgacaaccgt agttttggaa ggagaaggtc
     9841 caaatcaaat agccgaacga actggcgtac cagtagaaac gatcttgtcg ctgaatggga
     9901 tgagcatgga gaattatttc ctgactcccg gtgagacgct aaaattgaat tgataaaagc
     9961 aataaaaaag aacgaagctt ccactaaggt gaagcttcgt tcttttttta ttttccggtt
    10021 cttttttcgc gtacaaagta atggatcgtc atgtaaagca agaaaacgag tccaataatc
    10081 aatgagattc tcgtttcagg attgataaac atgaaaatca atacgatagc taaaaaagca
    10141 agtgtcacat aattcgtata aggagatagt ggcattttaa aaggatgatc atgcatttta
    10201 tctgcgtgct tctttttgaa gcgaatctcg ctgatcaaaa tcactaccca aggaaccata
    10261 ccaggcaaaa tacttgagct gtatacgaaa acgaaaatat tttctgactg gttgatgaac
    10321 attggtagaa gataatttaa gatcagacca attaagattc cacctgagat tgctacgaca
    10381 ggataaaaag gcgcaccgtg acgagatagt ttcagaaatt ttttagacat ctttccattt
    10441 atggcaagtg tatagaccat acgacttgcg ctgaaaatcc ctgaattgca acctgataat
    10501 gccgctgtaa tcacgacaaa attaatcagt cctgcagcaa atgtaatgcc gactttagaa
    10561 aaagtttgta caaaaggtga accaatctca ctcaattgat tccacggata gatactcaca
    10621 atgataaaaa tagcaccaat ataaaaaatc aagattcgac cgatagtgga ctggacagct
    10681 tcaatgatcg tctttttagg attctctgct tcaccagcag tcattccgat cagttcgatt
    10741 ccttgatagg aagcaacaac gatcgataat gcaaagaaaa atcctttgac gcctccagtg
    10801 aagaatccac catttttcca aagatttgaa atccctactg cctgaccatg attaccaaat
    10861 ccaaataaga ttaccccaag acctgcaatg atcatcaaaa tgatcgttat gactttgatg
    10921 atcgaaaacc aaaattctaa ttcgccaaac gccttgacag aaataagatt tgccagcata
    10981 agaaacaaga cgacaatgat tccgggaata attgccggca aatcaggcca ccaataattc
    11041 atataagatc ctacagcaat cacttcactt attcctacga caatatattg aaaaatatta
    11101 ctccaagcgg tcaagtagcc agccacagga tggatgtact cgctcgcgta atttgcaaaa
    11161 gaaccagtcg aaggatcaac gtaaagcatt tcgcctaaag cacgcataat caaatacaaa
    11221 aagaatcctg caatcgcata ggctcccatc actgagggcc ctgtccaacg tatggttgag
    11281 gaggcaccca taaacagacc gacaccgatc gttccaccta gtgcgatcat ttggatatga
    11341 cgggcactaa gacttcgagt taacttttct tcttccatac aaaaactcct ttcgttgttg
    11401 agtcaacaag aaaaaataat caaacgtttc aacacatgtt tttcattatt aggttagcct
    11461 tattgaactc ttgatgtgat tctacagaaa attttcataa aatgcgagga aaattcgaaa
    11521 aaaacttttt catcattcta cttgaaaatg ctttccattt tagttaaact atatttaaat
    11581 aaaaagttag gtaaacctaa aaaggagtgt aagatttgga acgtacggcc attgtaattc
    11641 aagacctttc agccgcttat caagggaaaa ctgttttgaa aactatcaac ttgacgataa
    11701 atccacaaaa aattaccggt attatcggac caaacggtgc aggaaaatca acatttatga
    11761 aatctttgct agagttgata ccagctacta ctggtaaagt caccttttct ggacagccgg
    11821 taaactcagt ccgtaaaaaa atagcctacg tggaacagcg aagtgaattg gatctttcct
    11881 ttccaattga tgtattaggc gttgtacttt taggaacata tccatcttta cgaattggac
    11941 aaagacctgg gaaaaaagaa aaagaacgtg caagacaagc tttgaaaaaa gtagggatgg
    12001 aagaatatgc caaaagacag atcagcgaac tatcgggtgg acagcttcag agagttttta
    12061 ttgcaagagc tctagcccaa ggagcagaat ggatcttttt agatgaacca ttcgtaggga
    12121 ttgatgcgtt aagtgaacga aagatctttg acatcttgca ggaattgaag aattcaggaa
    12181 aaacgatttt gatcgtccat catgatcttc ataaagtaga agaatatttc gatgaggtta
    12241 ttcttttaaa taaacagctg atcgcttccg gtccagtaca agaagtcttt acatcgaaga
    12301 accttcaatt ggcctatggt gagatcatca gacatttagt aaaaggagga gagaaaaaat
    12361 gatccaatcc tttattgatg gattaatgaa ttatcaattc ctgcaatacg ctttggtcac
    12421 ttccatgctt gtaggacttg cttctggagt catcggttct tttattattt tacgagggat
    12481 gtctttgatg ggagacgcta tctctcacgc agttcttcca ggtgtagcca tttcttatat
    12541 gtttggtttt agttacatct ttggagccac tgcttttggg atgctaacag cagcagtgat
    12601 tggtttcgtg actcagcgga gccgattgaa aaatgataca gcgattggaa ttgtctttag
    12661 ctcctttttt gcattaggga ttatcttgat ttcatatgcc cagagtgcga cagatttgta
    12721 tcatatttta tttgggaatg tcttagctgt tagggaaagc gatttgttgc tgacagcact
    12781 cgttagtgga atcgtcttga tatttgtctt tttcttttat aaagaattga agatcacatc
    12841 attcgatccg acaatggcaa aagcttatgg gctgaacaca tcgctgatcc attatctttt
    12901 gatgttcttt ttgacattag tggctgtagt cagtttacag acagtaggaa caatcttggt
    12961 gattgccatg ttgatcacac cagccgccac ggcttacttg ctaacgaacc atttactgaa
    13021 aatgatcatt acagctgcag gaatcggtat gctaagtgca gttgtcggtg tgtttttcag
    13081 ttatagttac aattggccat caggagctac gatcgtgtta gcatgtaccg cattttttat
    13141 ccttgctttt ttattttctc caacaaaagg aattttattt cagaaaggaa gcagatagat
    13201 gaaaacaaaa aaatcactct ttttaatctt ggcagttagt ttcttggtat tagccggttg
    13261 cggaaagcag gcttctgacc aagcggatga aggaagtaaa gaaaagttgt cagtcgtggc
    13321 taccaattcg atcttggcgg acatggcaaa agaggtcgga acagatcaaa tagatatcca
    13381 cagtatcgta ccagtcggaa cagatccgca tgaatatgaa gtactaccag aagacatcaa
    13441 aaaggcaagc gatgcagatg ttattttata caacggtttg aatcttgaaa caggtaacag
    13501 ctggttcgat aacttgatgg aaacggctaa aaaagaagaa ggaaaagatt attttgcagt
    13561 tagcaaaaat gtagaacctc tttatttaac tagcggtgaa gaacatacaa aagcagatcc
    13621 ccatgcttgg ctagacctat ctaacggaat aaaatatgtg gaggaaatcg cacgtatatt
    13681 ctctgaaaaa gatgcagaaa atgcgacact ctataaaaaa aatgcagaag catatgtgga
    13741 aaaactaaaa gaattagata cacaagcgaa ggaaagtttt gcttctatcg aagagaacaa
    13801 aaaattatta gtaacaagtg aaggggcatt caagtatttt tcacgagcat atgatttgcc
    13861 agcagcttat atatgggaga tcaatacaga aagtcaaggt acgcctgatc aaatgaaagc
    13921 gatcattgat cagataagag caaaagaagt accggtttta ttcgtggaaa ccagtgtaga
    13981 ctcaagaagc atggaacggg tagcaaaaga aacgggtttg aaaatctatg ataaactgtt
    14041 cactgattcc atagcaaaag aaggagaaca aggagattct tactatcaga tgatgaaatg
    14101 gaatattgaa acgatccatg aaggtttgtc acaaacaaaa gaaagctgaa aagtgcaagg
    14161 tctagcttga ggagcagacc ttgcattttt tataagagat atgaaaaaaa attatgctgt
    14221 ttttcgtcgt acagatacct tttttgaaga aattccgtta tagttagagt aaggaaggaa
    14281 ggtgtggcta tgaattttac acgtaaagat aaatatacga ttaaagaaga agtctttcga
    14341 ttgatcagcc agcaggaaaa taccgacaag gatcaagacc ttcaatttct tgaagcagaa
    14401 ttagctaaat tattcatagc aaatcaaaat gagacaacag aaccagatga agagctgtac
    14461 ataggcaaat ggttgtttga tgaatttcag cctttagatg aaaataccgg tatcggttat
    14521 tttgtgttcc tttctccagt cggcttcaca cctggcgtat tagttaaaca aaacgacgaa
    14581 ataaccgtca aagcatggcc tgcattattt tcagtcatca aagcagaagg agtaatcaaa
    14641 aagatccagt cggcctatta taaaagtcaa tttattgcca acagcaaatt aatgaatatg
    14701 ttttataatc gtctagtaga aattgctagg cctgggaaaa tcccgcaaac attcacgtac
    14761 acacagcaaa aaaatctcta tgaagaagta ttgtattccc gcgatctgtc tgagttttcg
    14821 ccatgggaag aggtaaggct ccatgaactg ttaagtacaa aaccagtttc attgaatttt
    14881 tttaatgcat tagataacaa aagaaaatta atttcaacaa attattggga agtatttgct
    14941 gcaagaaaaa aatggatgga agaaaagaag attgccgatc agcagatcca gtttgtccag
    15001 ctgaaaaaag aaaaactata ttatgggtta tacagccgtt aacagcaagc gaggaagacc
    15061 atgacattaa aagaagaaat catacaagaa agcaaacgat taggaattga caaaatcggg
    15121 ttcaccacgg cggaaccttt tgattcatta aaagaatcgt tggaagaaca aaaagcagct
    15181 ggccatactt ctggctttga acatccaaat atcgatgagc gtctttatcc agaaatgaca
    15241 tttgatcagc cgaaatcgat catttccatt gcacttgcgt atcctactag gatccatgaa
    15301 gaagtgccta gggatgaaaa acgcgggcag tttgctcgtg cttcttgggg gatcgattac
    15361 catgatatac tcagagaccg tttgaaccga ttgatcgaat tcatccaaac aagagcagaa
    15421 aaatggcaaa aagaagagga atggcgtcta gctccgcaag tagatactgg agaactgatc
    15481 gatgtcgctg tagcacagcg agctggttta ggatttattg gacgaaacgg attgttgatc
    15541 actgaagagt tcggttcatt tgtctatctt ggtgagatcg ttactaatat tgtatttgag
    15601 cctgataaac caggagaatt tggttgcggt gactgtatgc gctgcgtgac tgcttgtccg
    15661 acaaaagcat tgcttggtga cggaagaatg aatgcccagc gatgtttgtc ttatcaaact
    15721 cagacaaaag ggatgatgcc ggaagaatat cgtaaaaaaa tgcgaaatgt catttatgga
    15781 tgtgatatct gccagttagt ctgcccttac aatcaaggaa aagattttca ttttcatgaa
    15841 gagatggaac caaaagtaga cgaagtatat ccaaaattaa agccaatgct ttcgatgtct
    15901 aataaagact tcaaacagca atttgggcat ttagctggtt catggcgtgg gaagaaacct
    15961 ttgcagagaa acgcattgat tgctttagct aatctaggtg atcgaacggc attgcctgag
    16021 atttcagaat gtttaacaga tgtccgccct gtcatcagag gaacagcagc ttgggctatt
    16081 ggacgtctag gaacaaaaga accggaaaaa tggcttgatg tattagatga aatattagct
    16141 aaagaaacag aagaagtcgt tataaacgaa ttggaacatg cccagaaatt attgcggaga
    16201 aagttaaaat aaaaaaatag ttaacattat tcaaagtttt tgtccagtaa ttgttgtaac
    16261 aattactggt ttttttgtat tttttacatt tttttaagag aatttgataa aaatcaaaaa
    16321 tttttttgct tttttttatg gattattgca gtgaatgtta cagaatctgc gtttttcatt
    16381 caaaaa
//
LOCUS       DACDTH010000063        16219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDTH010000063 DACDTH010000000
VERSION     DACDTH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.063"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(120..617)
                     /locus_tag="IWK54_002187"
     CDS             complement(120..617)
                     /locus_tag="IWK54_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9594291.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(747..1457)
                     /gene="budA"
                     /locus_tag="IWK54_002188"
     CDS             complement(747..1457)
                     /gene="budA"
                     /locus_tag="IWK54_002188"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9594292.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1470..3134)
                     /gene="alsS"
                     /locus_tag="IWK54_002189"
     CDS             complement(1470..3134)
                     /gene="alsS"
                     /locus_tag="IWK54_002189"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9594293.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3342..4004)
                     /locus_tag="IWK54_002190"
     CDS             complement(3342..4004)
                     /locus_tag="IWK54_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9594294.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(4014..4829)
                     /locus_tag="IWK54_002191"
     CDS             complement(4014..4829)
                     /locus_tag="IWK54_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9594295.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5090..5533)
                     /locus_tag="IWK54_002192"
     CDS             complement(5090..5533)
                     /locus_tag="IWK54_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9594296.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5667..6005)
                     /locus_tag="IWK54_002193"
                     /pseudo
     CDS             complement(5667..6005)
                     /locus_tag="IWK54_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5993..6370)
                     /locus_tag="IWK54_002194"
     CDS             complement(5993..6370)
                     /locus_tag="IWK54_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9594297.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6594..7787
                     /locus_tag="IWK54_002195"
     CDS             6594..7787
                     /locus_tag="IWK54_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9594298.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7953..8375)
                     /locus_tag="IWK54_002196"
     CDS             complement(7953..8375)
                     /locus_tag="IWK54_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9594299.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8958..9452
                     /locus_tag="IWK54_002197"
     CDS             8958..9452
                     /locus_tag="IWK54_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594300.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9614..10786)
                     /locus_tag="IWK54_002198"
     CDS             complement(9614..10786)
                     /locus_tag="IWK54_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9594301.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10992..11807)
                     /locus_tag="IWK54_002199"
     CDS             complement(10992..11807)
                     /locus_tag="IWK54_002199"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9594302.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11957..12403)
                     /locus_tag="IWK54_002200"
     CDS             complement(11957..12403)
                     /locus_tag="IWK54_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9594303.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12400..13404)
                     /locus_tag="IWK54_002201"
     CDS             complement(12400..13404)
                     /locus_tag="IWK54_002201"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9594304.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(13444..13773)
                     /locus_tag="IWK54_002202"
     CDS             complement(13444..13773)
                     /locus_tag="IWK54_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9594305.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(13899..>16219)
                     /locus_tag="IWK54_002203"
     CDS             complement(13899..>16219)
                     /locus_tag="IWK54_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9594306.1"
                     /translation="IKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFTFLG
                     SLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQLIDP
                     TEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVAVNG
                     SMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNTIDS
                     VTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKGNVS
                     NFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVTGYS
                     FAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQDLTT
                     NTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKTNAT
                     VSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNEKGV
                     EVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGSKTY
                     DGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTNSGI
                     DKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVTGVV
                     LGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPSTGG
                     IGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4640 a         3236 c         2884 g         5459 t
ORIGIN      
        1 aaatcaataa cacttgtgat tttataaaaa aataaaaaaa ccgaaaaaca gaaatgacca
       61 ctgtctttcc ggctcttttt attcttgatt tctcttcttt tttgtttcaa tagacgattt
      121 tagttattaa taaatgaaaa agcatttata ttaaaatttg ctaataaaag atctacatat
      181 ttttctagaa atgcttgatc gatttcatca taccctttcg ttactccgca atcgatatct
      241 aaaacaccga ttaaattacc ttcttttacc attggcacta cgatttctga ttgtgcggct
      301 gaatcgcagg aaatgtaatt tgcatgggtt ttgacatcat caacgatcaa tgtttcttgt
      361 ttttcagctg attctccaca aactcctttt cccatcttga tacgtgtgca agaaactcgc
      421 ccttggaacg gtcctaaaat caattcatta ccatcgaata aataatatcc tgcaaagaca
      481 gtctcaggca aagtctcatt caacaatgca gatgaatttg ctaaatttgc taaccagtta
      541 tcttctgctt ctaataatcc ttgttgctgt aataataaaa tttcgtatgc atcttttttc
      601 tgtttttctg tccacatgat aaaaaccgct cctttttgat ctatggagaa gattatagct
      661 cagttttttt gtaaacgcta ggtttcctcc tcaaaaaaac gagatacgtc aggttaatca
      721 ccttgaacgc atctcgctgt ctttttctat tctgcttgtt ggatttctgc tgctaaatca
      781 gcataatcaa tcgtttcgtg aacaaaagtc ggatcatcaa ctggaaaatg ctgtacaagg
      841 gacgagatgc ggctaatctc aacttttcct tcagccactt caaaatccat tacatgtccg
      901 ccaaatgtat gtgtatcatc gataaaatgc aggtgaaatc ccgctgcagc aactccttgg
      961 aataattcag gcgtaaagaa cccgactaat gttccttgga tattgctttg ttggaattct
     1021 ggttgagact ctgaaatttt tgctagtctt ctgtaaggtt tttcttgctt cggcatgata
     1081 cggatgtgca tatttttgaa taatccagtt atcttcactg ccaaaaatag gttttccccg
     1141 gcttctctta ctaatatgtc ttttttcaat tgttctccat ctgcagtttt tgagacagaa
     1201 aaactagtat ctggcgtaaa atctgtaatt gctgcatagg gggtaagttc acttccatct
     1261 aattgggcaa ctgagccatc tgatcttcct tgataagcta cgccatctaa aaagatgact
     1321 tcaccatcaa gcccatgcaa tgttcccaat cccagagttc ctttttctaa tatatgagca
     1381 atcgtttctg ttccatccat caagccggcc atcaaagctc ctaatgtccc gtgctggtat
     1441 aagatttttt cagtcatgtc gacacttcct taatagaatt gatctggtaa gattgtttct
     1501 cccaatttct cattatcacg gtaatcaatc ggaatatcaa taattacggg tccttctgtc
     1561 gcaaatcctt cttctaaagc tttttctaat tcctctacac ttgtagctcg gattccttta
     1621 gcgccaaaag cttctgcata tttcacaaaa tcaactggac caaagtctac acctgacgcc
     1681 cgctgatatt tcattttttc ttggaactct accatattgt agtgtccatc attccaaatc
     1741 aaatgaacga tattcaattt tttacgtacc gccgtttcta aatcctgggc agagaataaa
     1801 aatcccccat cgccggaaac tgatacgatt tgtgtatttg gacgcaccaa tgcagcagaa
     1861 atcgcccatg gcaatgcaac accaagcgtt tgcataccat tactgaataa caaatgacga
     1921 ggttcataac ttctaaagtg gcgtgccatc caaatataat gactacctac atctacggta
     1981 acagtcatat catctgttac ttttgactga agcgtgttaa taacttctaa aggatgcaag
     2041 atacctgctt ctccttttga ttgaacaatg tcccgctccg ttaattttgc ttgaagagat
     2101 gctaaatatt cttttgcatc ttctgacact tgttgaggct ctaatgaacc tgtcaataaa
     2161 tcaagagtgg ctgagatatc accgatcaat tctctttcag gctgcataaa aggatcgatt
     2221 tcagctggcg cttcatcgat cacaatgatc cgcgcatctt tttccgcatt ccagtttctt
     2281 gcttcatatt cgattggatc atagccaatt gcaataacta gatcacttcg tttcaaaagc
     2341 atatctcctg gttggtttct aaataaaccc acacgaccaa agaaatgcgc ttctaattca
     2401 cgtgagatca ctccagctgc ttggaatgtc tctacaacag gcaatgctgt cttttcaact
     2461 aattgcctga tggctaacgt ttctttttca cttgatcctc gcatacctac taatagtacc
     2521 ggtagttttg cttctctaat gcgttcagct aggtaacgga tatcatctgc agaagcagaa
     2581 cctaatttag gatcgctcaa tggcttgatg acattacctt ggacaggtgc atccactaca
     2641 tcttgcggga tactgataaa gctcgcgcct ttttttgaac tcttagccat acgataagca
     2701 ttggcaatca cttctgaaat tgtttctgga tcttggattt ctgcactgta ttttgtgatt
     2761 ggctggaaca gtgctgcatt atccatgctt tgatgggtca acttcaatag atcgcttcgt
     2821 tttacttgtc cggcaattgc taaaactggg tcaccttctg ctgtcgctgt taccaaacct
     2881 gtagctaagt tactagcacc aggaccactt gttgcgataa ctactcctgg ctctcctgta
     2941 atacgtccga ttgcttgtgc cataaatgca gcattttgtt cgtgacgagt cacgattaat
     3001 tcaggacctt gatcctctaa ttcattaaat acaccatcga tttttgcacc tggtataccg
     3061 aatacataat caacatgatg attaatcaaa ctttctacta cagatttcga accttgtttc
     3121 attttttcag ccattgaaca tcgtcctttt tatgctttat ttttaattat tagttagaaa
     3181 taatcacaaa taagtgatac agtttctaaa cctgtttcat acaaaagtat agcacgtttt
     3241 atttcatttg tttagccata tgtttcaaaa aatgaaaaag aatgacagtt tttacataaa
     3301 aaaaactgtc attcttaatt gaaagtttca atttataaag tctacagatc catagttctc
     3361 atcaatttac gattgttgaa gtaaatcaaa ccaaatacga tagaaggacc tagtagaata
     3421 caccattggg gctttacgaa aaagaagaga aacaaacttg tcaatagtaa aaaatctagt
     3481 aaactcacat accatttttt caacgtatag tagaacgcta tccgtagaac ttgatttggt
     3541 ttcgacgctg gatttctcac ctgaaaatac cagcagttgg tgctaaagga aactgataaa
     3601 caagcaagta aaacaaacaa cggaatcaac cattttccat aagaaaaacg tataaaaaag
     3661 aaacaatcaa aaatcattat cacactaact atccaagcaa atagccaata aaaaaatccc
     3721 tttagccaaa attggcgaaa catctttatg aacaaacgta ctggtgaaaa actattttct
     3781 tttgctaatt cttctgtcat acctatcatc acgatgcaac ctggaccaat aaaaagtaaa
     3841 gaaaaaataa aggaaaagac attccttcca tctatcgaca aaaggaacgc agctgctata
     3901 tacaagatat tgacacacca aaaacaaagg ctacttgcca atgattgata caaccaataa
     3961 aagaattcca tataaacatt tttgtcaaaa cgctgctctt tcataatcac ctcctattcc
     4021 ttcattcctg atgaagcgat cccttctaca aaatattttt gcatcgctaa aaatataaaa
     4081 gcaacaggaa gaacagaaac aactgaggca gccatgatta atgcgtactc tgcatcaaat
     4141 agcccaataa acattttcaa ccctaactgg attgttttat tttccgtaga agacaaataa
     4201 atgaatggcc ccatataatc gttccatgta ttaacgaatg tgatgatcgt caaactggca
     4261 atcgccggtt ttgtaagagg taaaatgatc ttgcgataga ttccccactc tgataagcca
     4321 tcgatccgcg cactctcaca taaagaatct gggatactac tgtaatattg cttcaacaag
     4381 aacacaccaa aagcattgaa cgcttgtaat agcaccaacg accacaacgt atctgttaaa
     4441 cccacttttc tcatcataat gaactgcggg atcatataag actgccaagg gacagcaatc
     4501 gtgccgatat atgctaaaaa taaaatatcg cgccctttaa aatttagttt agaaaacccg
     4561 taagcagcaa aactagaagt aaacaactga atcaacgtga tcacaacgct taaaaaagct
     4621 gtattcttaa aaaatgtcac taaaggaata cgagaccaaa taaccagata attctctaaa
     4681 tgccaagttt tagggatcca ttgaatcgga atcgtgaaca cttcattatt tgctttgaat
     4741 gaagcagaaa ccatccataa aaaaggaatg atcgtcaata aagctaatac aatcagcaga
     4801 atcataccca accattgaat aaagggcatg ttttttgaac gaatttttgc tttttttctt
     4861 tcatttcgaa cacctccaaa gttataatct tcatttcttt gtaatgatta caatccagaa
     4921 caacggttat tatagctgat tcatttccgt tcttcttatt tttgtaagcg cttatttaac
     4981 aagttatgta aatctcttta tttttttgat ttaaaaatga aaaaatacaa aagcaagact
     5041 gtcatacgtc tcgcttttgt attgaaattt cttcgattag agatacttat tactgttcaa
     5101 aagcttcttt gaaacggtga atcaattcat tgactacagg tgataaaatc cgattctttt
     5161 tccagactag catgcagttc gtttcaatca ttgggtcaag cggaacgaat gtcactcgct
     5221 ttgaatgatg acttacagca ccttctatga ctaaagcagc acctacctga ttctcaatta
     5281 gcgcaaatac attgaaaatc aaattgtacg ttcctgtgat cagcaactgc tcctttttta
     5341 tgcctaacca gtcagcaaac attcgctgta tttctggacg ctttgagatc aaaagaggta
     5401 cattcgcaaa gtcttctttt gttatcacgt ctttttttgc taaaacagaa ttttctaaaa
     5461 tgagcaatcc ccatttttcc tttcttggta acaaaatcgt ttcgtatttc tctgtatcta
     5521 ctggatctag caaaatcgct aaatctaaca ggcttttttc tagtcactcc gtgatatcat
     5581 cacttgttcc gctgtggata tgaaattgaa cgctgggata atcacggatc aacttttcta
     5641 gtaatagtgc caatgtattc gaactatcag cttccacaca gccgatcgta aatcggccgc
     5701 tgaataattg tttttttctg ttctcgaatt cttgagtggt ttgattcgtt aaatctaaaa
     5761 tttcttctgc tcggcttttt tagaaaataa cctgcttctg tcaagatcat tttgcggttt
     5821 tcgcgtacaa acagttctgt tcctagttac tcttctaact ccttcagttg tcggcttaag
     5881 gttggttgtg taagatgtag aacttctgcg gcttgagaga ttgttccttc ttctgcaatt
     5941 gtccaaaaat attttagtaa gcgtaactcc atagcgatgc tccttgattt ttttacttga
     6001 tacattccat tttatttcta gataacacac gatcatattt cttgattttt ccttgtaaac
     6061 gttccaaaca ttcttgcatt tcagcaattt ttttcgtcaa aagttcactt tcttcaatca
     6121 aaagcttacg ccgctcaaat acagttgctt ctccttcgct agataggcga atatattcaa
     6181 tcaaagactc gatcgataat ccggcatttc gcatacagat cgtaaagtcg acccatttta
     6241 ggtcttcttc tgtataatca cggattccgc cttttgtccg attaactggt ggaatcaacc
     6301 cgattcgttc ataatagcgc aatgtatcgg ccgaaatgcc cttttcttca ctgacttgtt
     6361 tgatattcat ctgatcactc tttctcttaa tttcttgcca ctttacttat gtattttatt
     6421 tattctacaa ctttaagtgt actccaagtc aatactcatt tctattaaaa ctaagctgga
     6481 ttatctatat cggttgtttg tgtattattt caaataaaaa agtcttgttt ttttattctt
     6541 ttttggatat acttttaaca atttagggaa attttaggaa aaggggtata aaaatgacaa
     6601 cgtatcagcg aaaggtatta gtatcgacat cggcgggaat tgctttagag aatatggata
     6661 ttatgttttt agctttttca ttatcttcaa tgattacttc ttttcatatt agtggtacgc
     6721 aagcgggttt catttcaacg atcactaatt tggggatgct cattggaggt attttttttg
     6781 gcatcatagc tgataaatat ggacgtgtga aagtattttc tcaaacggtc cttctgtttt
     6841 cgattgcttc tttattgatg tattttgctt caaacatcta cttggtttat ctctttcgct
     6901 ttatcgcagg tgtaggagcg ggaggagaat atggggcgtg tatgtccttg atttccgaaa
     6961 gtttttcaaa aaaacaaatt ggacgagctt cttcggttgc tggtattggt gcgcaggtcg
     7021 gtgcggcttt ggctgcgatt ttagctgctg tgatcattcc aacgtttggt tggaagatgt
     7081 tgtatgtcgt aggtgtactg ccggtgttga tggtcttgtt cattcgtcgc ggcttgactg
     7141 aaccaaagga attcaaatct gtacaatctg ccgaaaagaa aaccaaactt agcagactgt
     7201 ttgaatcaaa acatcttgct tggcaaacat gtggccttag tatcatggta actgtccaaa
     7261 ttgccggcta ttttgggtta atgaattggc taccttcaat tatgcaaaat cgattagggc
     7321 tttcggtttc tgggtcttca ttgtggatgg tcagtactat tttaggcatg tctttgggga
     7381 tgatgacttt tggcataatc atggataaat tggggcctaa actagccttt tctttgtttt
     7441 taatttgttc atccttatct gtctatctac ttgtgttagc ccatagccag tggagtttgg
     7501 tagcagccgc tgtgatagtc ggctacttta tcaatggcat gtatggtgga tatggcgcag
     7561 tgattagtag tttgtatcca acagaaatcc gcgcgacggc gaataatttc gttatgaacc
     7621 ttggacgagc tgttggtggt ttttcttcta tcgtcatcgg tttcttgatg gatcattata
     7681 gtttaactgc cgtaatcttg tttttgagcg gtatttacct agtcagtctg ctggtcttga
     7741 caactcttac tggtgttaca gcattaaggg aaagcttgca gcattgatag ttgagtctgt
     7801 aaatgacaag aaagaggtgt ggaaaaagct tgcatctgcg gtaataagac cagccaaatc
     7861 aaaaacatga gacactgttt ccgtttggct ggtcgtatta cttgatgcag ggcagctttt
     7921 tcaactgcta atttccttct atctggcggc gactattctt tgacgtccgt catttcaaag
     7981 tactttacta ataacttagc ataacgaaaa actgtggata cactcgtata ttgagcgtct
     8041 gcataatctt ccaaattttt ctcaggatgt tgatacatga attcaatcaa atgataatta
     8101 tatgattttc tgtagaaatg aagataaata atgtagattg ggaagttctc ctgcttttcc
     8161 atagaaaagc gccgattatt ctcattcttc aaaatgacct tatcaggata ttgttggaac
     8221 aattcttcta aatctgttag ataattcaag atagacttac ttgatacctt caattcgttt
     8281 tgtagttctt ctattgaaca ccaacgttct gtttcaagca gtaaatgaat cattttaatt
     8341 ttcttttgag aaacttttgt taaaaaagtc tgcatatttt ctcctcctgt atctacttta
     8401 ctatactaca tacaagaaaa aaacggatac aaatatcata ttgtcacacc gtcaatagtg
     8461 cataaacctg ttattatttc tctttttttc ctaagtattc tattgatatg aatcatttaa
     8521 tcgtttatta tataaatgtc aataatctaa taataaaata acgtacatat tttattattg
     8581 catacttaat aacagaacta tagcaaaaaa tcccgtgtat caacacggga ttagattaag
     8641 tttcctcaat cactattcat aacacaatta ttataacaag taagtaaaca attgaaaagg
     8701 acatttattg aaaacggttg ttttttactt aaggaggaaa tttagtgaaa aaattagttg
     8761 cgttgttttt ggtcgcagga agtttgttga gtgggtgtgg tgctactacg tctactactc
     8821 aaagcacaag ttcagctcag gcttccagca gcagtaccac gatctccgaa agtacggcag
     8881 attcatcgaa agaacaaatt gaaaagaaaa aagaagaaga gaaacaaaaa gcggaaaaag
     8941 ctgcactaga aaaaaaagtg acagaagcgg aatctgcttt gtcacaagct gaagcaaatc
     9001 ctacaaaaga aaactatgca actgcttcag cagctatcca agcgctacct gaaaacaaac
     9061 aagagttgac aagccgtctt gctgcaattg attccacaat caaagcaaaa gaagcagaag
     9121 aagaacggaa aaagcaagaa gaaattgcac gacaacaagc agaacaagcg caacaagaag
     9181 caatcgcaca gcaacaagca gaacaggcga ggcaagaagc aatcgcacag caacaagcag
     9241 aacaggcgcg acagcaagca gcagctgaac aggcttctca gcaggcacaa gccgcagctc
     9301 ctgagcaaaa taatgaacaa actgtctatg tcacaccgac cggttcaaaa taccatacac
     9361 ataaatgtgg aaatggtacg tatacccctg ccactttaag cgaagctcaa gctcgtggtt
     9421 taaccccttg ctccaaatgt tatggaaact aattcaaaag ttatactata ataataaaat
     9481 tcttctgaaa cataaaaaaa tagaagctgg cgttccagat agtaaaccaa aaatagcagt
     9541 ttacacgtcc atttcgtcag cttctctctt ttttcattta gtctgttccc tctgcttttt
     9601 tggacatctt cattcattct ttctcaatca aatagtacac ctctttttct tttcgcagtt
     9661 tatatcttcc ttttttcaat ttcattgaaa actgtttcga atgtttatgt tttacatgga
     9721 ttttaagcag gagctcctca tgttgcaagc gatagttcct gccttttaaa tcagaaaatc
     9781 tcactagtcc gtattcatca gaagctttca caaatggaac tttttctcta gtaatatagt
     9841 gtttgccgtt gagcgttttt acagaaaaga ccattccttc gattggtcgt ccgtttgtgt
     9901 tctttacata aaaagataaa ggatagtttc gcttagcgat ggccaaagaa tctaaataag
     9961 cgatgagtag ccaaactaag ccgattgatc caagtagtat accgataaac caaataaaaa
    10021 atttccattt tttccattga tcaattcctt ggatcgcttt ttttgcctct tttgcttgat
    10081 aaggaatacg atgccccgtc acaagcagtc gatgtgtatt gaccatataa ggagtgcagg
    10141 taagcaaagt cacgagatct ctgccttttt ggatacccaa agaatctact tcgtcaggta
    10201 agaccacttg gattttttct acttgatacg ccaatgtctt tccattgatc tggatgaaaa
    10261 actggtctcc ttttttcaac ttaggcaaat ctgtaaataa ttttgcttct gggagtccac
    10321 gatgcccaga aagaacggca tgggtgtttt tcccgccagt cggataagaa cttccttcca
    10381 ataatgacgt tcctttttgc aaaaatattt ccgttgtctg atcgaatata ggtaagctga
    10441 cattgatttt tgggatttcg atgactccaa gcatatgttc cataaaaaag gcattactag
    10501 gtaaatcttt taatgctttt gcatcaactg cttggttgaa actgcctatc cctggtgata
    10561 cgttttgctc tgccaactgt tggtttttct ttgtcatttt ttcctgctgt ttttttattt
    10621 cctctgaatt cttattcgat gcttgctttt gataatggat gatcatttgc tgggcaagaa
    10681 aatcattcag tgaatcttct acaaaaggat aagaaaagat cccgatccca acgactaaaa
    10741 tgattgccat gaagatgtcc agtattctta ttctgctttt attcatcatg tccacctatt
    10801 ttctaatatg tatatgaaga ggttgtgaaa tcagcgcctt gacctgagta ttagcgaagc
    10861 aaacggcaaa atagttcctc ctattttgcc gtttgttgag cgaataccaa gagaccgacc
    10921 acttgtcaca acctttctcc ccatttgaat cactatcgtt tatttatcgt atgatttttg
    10981 tttccaatag tttaagcttc tgtttcttga tggcgttttt tgaaatagat cactgctcct
    11041 ccaactaaag cagtacctac caagatgaat acgatgatcc ccataccacc agttgaaggc
    11101 aagattcctt taggttggtt atcgatcgtt gtttcatcag aagttgttgg atcatcatct
    11161 actgtaaatt cagtgtacgc agcttcattc ttaggtaatg cgtagccttc tggtgctttt
    11221 gtttcttgaa ggcagtatgc cccttgtttc agcccttcga ttgaaaattt cccttctgaa
    11281 tcagaaatca atttcacttc ttcccagtca gaacctgtga cttcatctga ccaagcatat
    11341 gggtattctc ctgaggcaag gtttgctatt tttttcatta atttaacagt tggatctttt
    11401 gctcttagaa tagaaaattc tgcatcggct aatttttctt gcgtgttcac atcttgtttg
    11461 atgaagtgga aatcaccgta gttttggaca ttttctacag taatcgtgtt atccgtagtt
    11521 gtattttccc gattgactac accgtttgga ttcccaagat tggcttcatc tgcgccatgg
    11581 atgataccgg ttgtttgttg caattttccg ttttttacta taaaagtagt ttctttttgt
    11641 ggattggtta attgataacc atctggtgct tttgtttcaa ctaaagtata attgccgtct
    11701 attaatccat tgacactgat ggatccttca gtatcagttg tgaatgtcaa cgctgactca
    11761 gcaggatcat agagagcgac accagtgttt tcatcaaaac cggtcacgaa ttgattctct
    11821 gaatttttga tctggaattg cgcccctgct aacttatcta cccctgaaaa gttcccagct
    11881 ttctggattt tgatattgct ttctttgatg acatttttag catataggtg aatcgtgtct
    11941 aaaggtttgt cagggtctag ggtaagctta acacgatatt tggcgacgtt tcgacaccgg
    12001 gctttggtgt ttctacaaag acgtatacag catctttccc atcagattta cctggaagag
    12061 tcaggttagc taatccttca ccagaagtcg ttacacttgc gaagggtgta gcgtctgctg
    12121 gaactgctgt gtctttactt ggatagtacg tagcagtgat gtcatatacg ttgaattcaa
    12181 cgccgttcaa cggttcgcta ttcggaaatg tcaattccgt tccgttgttt atttgatttt
    12241 ctggtgctga agtgaatttc aatttatgca aattaaccgt aacattttct ggtgtaggtg
    12301 atacggctgc agaaacctct gcagttccta ggccgagaac gaaaggaagc agcatgatag
    12361 cagttcctaa aaacgtccat atttttgttt tgatgttttt cattttattt ccctcctatt
    12421 gtttcctttt tgttgttttt tataaaacca agctgtccct gttgaaagga ctagcaataa
    12481 ccctaaaatt gagaaacctc caagcatctc ttcgcttgtt tttggaaagc ttcctccatt
    12541 tttttcattt gatggaccat ttgtctgcat ctgaactggc gtttttccat tattcgtcgg
    12601 atttgctttt ggttcgttga tgatttccaa tggtatagga tgaagcggat cataactgcc
    12661 tttttcaact tcaaagagaa tggttgattg actcaatata tatcctttag gcggtttaat
    12721 ctccactagt tcgtaacttc caaaagacaa tccatggaca gaaaatgttc catctttact
    12781 ggaagtaaaa atcgttgctc ttgaatcatc tttggctacc caatgattta tcccgttttc
    12841 ttgcgctaag tattggtcat tttttttgac tagaaattga gcatctgcta gtttttgttt
    12901 cgtttgccga tccactttga caaattgttt gcctcctgtt tggatggcaa gatgtgtttc
    12961 tactgtttga ttgtcagtcg tcaaacgtac atcgttatca aatgtcgttt tttcactgtt
    13021 ttctttcaat gtcatttggt aacggatcgt taattttttt ccagcaaacg aactaagatc
    13081 ttttccagaa tctgataaga caaaaccatg ttttattttt tgtgtgtgaa atgtatgaat
    13141 cttgtctcca ccaatcaaaa aatcgatcgt ttctggttct agccataact gaggatcagc
    13201 tgtatctttg atcgtatagg ttcctatctc gttgatgttc tcaggaattg ttacggtgat
    13261 gagataaggg actttatccc catctgcaaa atctgtctgc ttattctcta ctgtttttgt
    13321 taatgtgagt gcagcaggta ttttttgttc gctctttgga tacaagtgga tggtcgtcaa
    13381 tttatgatta gatgaatctg tcacaggaag cacaacgaca aatggtgctg atttttcttt
    13441 tacttaatct gaagtcttta tttccgtaaa tcggtaaaca gcgtcacgac cttgctcatc
    13501 tttgtccgat gcactaaaag aagcaatgcc ctcttcctcg tctactgttt ttgtcacatt
    13561 ttctgccagt attttttcac tatcactttt ttgtgctagt tttctttgtg cgtcttccac
    13621 actacttcct tctgaccgca gcttgtaaaa ttcatccgag acatcatata tagcgaaagt
    13681 aacgccgttc aaccctttta ctccttgaaa gttatccggg atatttattc cgtcgttttg
    13741 actgatactt ggcatcttat cgctttcgaa cattcgatta tgcaaagtaa atgtaatagg
    13801 tgattctcta gctgacacag tcggagaacc tataaaaaag accagtagga taaaaaagag
    13861 aacagcaaat atatccacac gtacattttt tgtttttttc atcgctgttc acctgctttt
    13921 ttcttacgat ggataaaata aaagaagcca gaaaaaataa agatcagtcc taaaaagaca
    13981 aagggcagta gacctatccc acctgtcgaa ggaagcaaga gagtcggatt gatcgtcaac
    14041 ttagcggtat ctgttgcgat aatcgtataa ttattgttta cacttccttc gcctaaacta
    14101 atcgttacta tttgtgatcc aatatctgtc cggctgttaa ctgttagggt ataatccaat
    14161 gattcgccta agacaacacc ggtaacgtgg agatttccaa ccgaaatcga gtgactttgg
    14221 gcatcataat taaaggcagc gtcatcgaca gtaatcactg cgttagcttt atagatggta
    14281 taagttcctg atgtgttggc tatatcagta aatgacaact tgggatttgc tgctttgact
    14341 ttgtctattc cgctattcgt tagtttaatt gtatagctgc cagcattaac catatttttt
    14401 gctgattgtg catctgctgt gtaatccata ctgctaagtg ccacttgtac tggagtaagc
    14461 gaactagtga caccctctgc tagcttgaca tggattgacg ggacacctgc ttttccatca
    14521 tatgtcttac tgccactgag ctctaccgca ccgctgattt gcttaatcgt atagacatca
    14581 gatgtctgca cgatagtaaa taaatagttt gttctggcag ctagtttagc tagtccgctt
    14641 tttgtcaatt gccaagacgt ataggttcca atttcagtag gcgcagttac ttcaactccc
    14701 ttttcattca tcgctttgaa atcagttgga ttccatgtga tttgatcaat actgagatca
    14761 ttgtttaaaa gggatgttga ttctgtttta tcattaaagg catatttaac gatcggtaca
    14821 acagaaagac catcatagta cttactgcca tttgcattga ttgatactgt tgcatttgtc
    14881 ttataatata ggatgacttc agaaatctgt tttccatttg cgtcctttac gattacattt
    14941 ccatttgtat ctaaactagc taaaacactc attgctgatt ggttctttgt ttctatcgct
    15001 gaaacagctg taaaggtata tccgttgata gtaggcgtat tagtcgtcag gtcttgtgca
    15061 gcacttccgt aagtaagatt cattatttta gaaggagcaa tcatctgtcc aaaatagtct
    15121 tgatacttaa tagtgattgg tgtttgtttg cctgtatagg tcaccgtatc gacgttactt
    15181 tctagggctg tgaaagaacc gtttgtttgt gcaaaagaat agcccgttac tgctgttgga
    15241 gtgataacca ctttgtcacc cacatggatt ccagataatg tagtcccgta cgttccgatt
    15301 tttgctgctc caccattgac tgtataagaa aaagttcctg tccgattcgc attgacctga
    15361 accgttgctg cttgataggt tggaaaattt gaaacatttc ccttaccatc aacgatctgc
    15421 ccatttttga tcgagtaccc cgtgaatgga atatcatttg ctaactgtat accgattgaa
    15481 aaagtactca tacctcctcc ggcaggaagc cctccccatg ttgagggtgt aaaagaataa
    15541 cttttatccg cattgggtgt aactgaatcg atcgtatttc cagcggtagc cacccccata
    15601 tagatacttt ttgggaatgt gatagtagta ccatttactg tggagccaat aaacagatta
    15661 tggatatctg gacggttatc aataaaagcg attgctttgg ttacgccttg taacgcagtt
    15721 ttatccgcac tcatcgatcc gttaaccgca acaatgactc catctttggc atatatcact
    15781 gtatatccat ctggtatggt atgtccgttg aaattcgcag tggccgcaac agtactaaat
    15841 gtctggcttc tacctgtttc tgaataagct ttctcccctg ctgcttttgc tgctgtgatt
    15901 tctttttctg tcggatcaat cagttgagtc gaggatgtgg ttgaggttgt ctcgttgttg
    15961 ctagactgat tggtgctttc tatggactgt atttcagaat agggtggact atttgtctcc
    16021 tctgtcgttt ggttagcaaa atttgacgtt tctgtataaa cgtttacata aatacttgat
    16081 aagctcccta agaaagtaaa aatcaagatg atcgaaatca taatgggatg taagtatttt
    16141 cttttagata cagctttgag tctagaaaca aaatgttttt gacatatacg ttggatttta
    16201 ttttgagttt tcttaatca
//
LOCUS       DACDTH010000064        16188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDTH010000064 DACDTH010000000
VERSION     DACDTH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.064"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            197..793
                     /locus_tag="IWK54_002204"
     CDS             197..793
                     /locus_tag="IWK54_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9594307.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(851..1333)
                     /locus_tag="IWK54_002205"
     CDS             complement(851..1333)
                     /locus_tag="IWK54_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594308.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1439..1636)
                     /locus_tag="IWK54_002206"
     CDS             complement(1439..1636)
                     /locus_tag="IWK54_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594309.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            1931..2689
                     /locus_tag="IWK54_002207"
     CDS             1931..2689
                     /locus_tag="IWK54_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9594310.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2749..3177
                     /locus_tag="IWK54_002208"
     CDS             2749..3177
                     /locus_tag="IWK54_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594311.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3223..3295
                     /locus_tag="IWK54_002209"
     tRNA            3223..3295
                     /locus_tag="IWK54_002209"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3256..3258,aa:Gln,seq:ctg)
     gene            complement(3445..3816)
                     /locus_tag="IWK54_002210"
     CDS             complement(3445..3816)
                     /locus_tag="IWK54_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9594312.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4031..4216
                     /locus_tag="IWK54_002211"
     CDS             4031..4216
                     /locus_tag="IWK54_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594313.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4299..4487)
                     /locus_tag="IWK54_002212"
     CDS             complement(4299..4487)
                     /locus_tag="IWK54_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594314.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4511..4774)
                     /locus_tag="IWK54_002213"
     CDS             complement(4511..4774)
                     /locus_tag="IWK54_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594315.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            4932..5189
                     /locus_tag="IWK54_002214"
     CDS             4932..5189
                     /locus_tag="IWK54_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9594316.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5260..5451)
                     /locus_tag="IWK54_002215"
     CDS             complement(5260..5451)
                     /locus_tag="IWK54_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594317.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5592..5912)
                     /locus_tag="IWK54_002216"
     CDS             complement(5592..5912)
                     /locus_tag="IWK54_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594318.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(5935..6369)
                     /locus_tag="IWK54_002217"
     CDS             complement(5935..6369)
                     /locus_tag="IWK54_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9594319.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6704..7342
                     /locus_tag="IWK54_002218"
     CDS             6704..7342
                     /locus_tag="IWK54_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9594320.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(7666..7749)
                     /locus_tag="IWK54_002219"
     tRNA            complement(7666..7749)
                     /locus_tag="IWK54_002219"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7713..7715),aa:Leu,seq:cag)
     gene            8277..8777
                     /locus_tag="IWK54_002220"
     CDS             8277..8777
                     /locus_tag="IWK54_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9594321.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(8967..9311)
                     /locus_tag="IWK54_002221"
     CDS             complement(8967..9311)
                     /locus_tag="IWK54_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9594322.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(9465..10385)
                     /locus_tag="IWK54_002222"
     CDS             complement(9465..10385)
                     /locus_tag="IWK54_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9594323.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            10572..11063
                     /locus_tag="IWK54_002223"
     CDS             10572..11063
                     /locus_tag="IWK54_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9594324.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(11087..13462)
                     /locus_tag="IWK54_002224"
     CDS             complement(11087..13462)
                     /locus_tag="IWK54_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9594325.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            13788..14414
                     /locus_tag="IWK54_002225"
     CDS             13788..14414
                     /locus_tag="IWK54_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9594326.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            14437..15141
                     /locus_tag="IWK54_002226"
     CDS             14437..15141
                     /locus_tag="IWK54_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9594327.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            15333..16166
                     /locus_tag="IWK54_002227"
     CDS             15333..16166
                     /locus_tag="IWK54_002227"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9594328.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         4977 a         3148 c         2712 g         5351 t
ORIGIN      
        1 aaaattccta aaacacaaca atgaaagcga taactaagaa aatccttaca taacgatttt
       61 tcttattaaa attgtttttt gaacttcgct tattttttgt gagcatatct gtttacttac
      121 tttttgtaag gtgctatact tcatttcgta atcaattaaa aaaatgcaaa acgcaaatca
      181 aaggagaaat tttattatga caacttttac tgacactttg aaaaaccgcc gttcaatcta
      241 tcatctagga cgtaatgttt cactttctaa tgaagaatta acaactttga tcaaagaagc
      301 aattaaagaa agcccaactg ctttcaatgc tcaatcaaca cgcgcagtta tccttttcgg
      361 agatgcacat gaaaaacttt gggaaatcac tgaagaagca cttcgcccat tgactccagc
      421 agaagctttc ccaaatacac aaaacaaatt agctggtttc aaaaatggtt atggtacagt
      481 attattcttc aaagatactg acgtggtaaa aggtctacaa gaacaatttg aattatatgc
      541 agacaacttc ccagattggt cagaacaatc aaacggaatc gcaacagcta atacttgggt
      601 agcattagtt gataaaggat tgggcgcaaa cttgcaacat tacaacccag ttattgacga
      661 agcagttgca aaagaatgga atatcccttc aaactggaaa ctacgttcgc aattagtatt
      721 cggttcacca gaaacaccgg ctggggaaaa agaatacatg aacgatgccg atcgcttccg
      781 cgtatttgga taattttatt tgaaataaaa aacagaggtt gtgacaaaca tcttgtcgca
      841 acctctgttt ttattctgga tcaatcataa tatcaatctc tgcctcatcg tttaagcgtt
      901 gggacaaaaa tggaactaat tcttctaatg atgcagaaaa tttcagcatc ggtcctcgcc
      961 atccgtttcg gaaaaaattc atttttacga ttttagaacc ttttggctga tataatacaa
     1021 ttttattgat ctcagaaaaa ggaacacctt ttttgtcaaa gctattcaaa acaagtcctt
     1081 cttctgtgat tcctctactg tccaacaaaa aactaataaa aatcattgct gacaagatcc
     1141 cttttgccaa ttcatctgct gtaggatgac tgatgagtgt ataccaaatg actagtgaca
     1201 ctaagaaggg tatcaccaag tagacccatc gatttcgcac agtccgataa ataatttctt
     1261 ttcgaaccag ccaaagaaat aaaaacatca caacaacgaa taaaaacaat agaaccgtaa
     1321 agaatgttac catgacaact cgcctcgctt tttcttctcc agaaaatagg aaaagaatac
     1381 tgatttacct tctcaaagaa attggacttg cagcacgccg cacgtccaat tgttttcttt
     1441 attcaaatgt atcatatttt gcaacaacca tgcttgcttt ttcatctaat aattcttggt
     1501 cctctgcagc acattgggca actttgacca cggcgcagtt tgtcattttt gatacaactt
     1561 caccaatgac cgtttttgta aaaccaattg gtttgcatgc atattgttct cctaaattaa
     1621 tcatttcttc cgtcatcaaa atcctcctaa actgctggaa cttttctcca cattaccact
     1681 gctatttctt tggagattta actcattgat ttttatttac agttaaaaat caaccttaca
     1741 ggcattatag cgtactgttt tcgttttcac aattaaaaca gctgttgtat ttagaagaat
     1801 ttttttcaaa aaattaaaac atattcctta aaaactaatt atttcctaca tattaaaata
     1861 taatacttcc atttccaata aaaatacatt tataatagga aagataaaca gctgaaaagg
     1921 gagatttcaa atgaagaaaa aaacgttcaa aaaactattg tttctagggg gcacagctgc
     1981 tcttttatta agcggtacaa gttattacct acatatccat tctccagaac atacaatgaa
     2041 cactgcacaa aaaagttcaa cacaaaaaac gaaaaagaca aaagagaaag cagaagtttt
     2101 actagatgtt ccattagaga gccagtttga ttctccttct ttagaaaatg gctgtgaagt
     2161 cacttcgctt tctatgcttt tatcttttta tggttatgaa acaactaaaa accagttagc
     2221 tgagcaattg gattacgtcc ctgttttcaa tgcagatggg actcatggcg atcctaatga
     2281 aggatttgtt ggtgatatca gtggcggaga ctgggctatg gtcgtctatg ttcccccagt
     2341 tgcctccctt gctcagcaaa tcgtccaaac ggattatcac acttttccaa aaaccgatgc
     2401 tcaactcgat gatatcaaaa aagcactatc taaaggaaat cctgtctgga cttctgtgac
     2461 tatcaatttt gaagtcccag atgaaactga ttttatgact tggaccacaa ataatggaga
     2521 agtaagagtc actcctcttg ttcatgcttg tgtagtcact ggatatgaca aagaaaatat
     2581 ttatgtcaac gatccctatg gtgtaaaaaa tcgagcagta ccgatcaacg atttttcggc
     2641 gattttcact gcaatgggtg gacagatgct aactttggag aaaagctagt aattcaaaca
     2701 aaaatccctt atactcaaga taccaaacct ttgaagggag cgtagataat ggaaaaattc
     2761 acacataaaa aaatggatcc aaatgaaatt ccgattattt ttgtccgtga ctgcaaggga
     2821 aatgttcaag gtaaagtctc tattaatgag tggaacgagc gtcgcagacc tgctaccctc
     2881 aatgaactgg aaatcaaatt gtatcgtcaa tctcttgttt attacgcaga tcaagagtac
     2941 gagaaagcca ccgatttatt gaaatttcta attgctcgaa cagagtatac tcgctttgaa
     3001 tatattgaac gtttagcaaa catctatcat attatgaatg aacctgtcaa agaatatcag
     3061 ttattggaca ccgtcttgtc agtagcagaa ctgattgccc ttcctgctgg attagaaaag
     3121 aaactagtac gccgcctttt acgagtaaag caacaactat ccgatcaaga aaaataactt
     3181 gcgttttttt aggaaagtgc tatgattatt ttgttgaata aatgggatgt agccaaactg
     3241 gtaaggcact tgactctgaa tcaagcaatt gtaggttcga accctgccat cccaatttga
     3301 tgtgcttgct gaaaaaatga accccctgta aaagaacaat ctttttgcag ggggattatt
     3361 gtattatttt tactacttgt atttagttta cataattaaa ttatgaataa taaaatcggg
     3421 cgtattctta atacaaaaac tgatttaccc aataattgct aatgctttct ccggaacttt
     3481 ttctctagga acttcctcat acgtttctat gtgtgctcct ttattgctaa ttttcaaata
     3541 atgattttct tttaacacag aaagaccatt aaattcaacc gttcgttctt tcccattggc
     3601 atcagcagct ttctgcacat accttgctgt accatattca tttacttcct ctggtttttg
     3661 tgtctttaca tagacctctc ctttttcaac taatggattt agctgatcaa gtataccagg
     3721 gatttctcca taacttcctt cagtataata tttcagtcca aatagcaaaa taccactaat
     3781 cgccgccagt ccaatcaaat atttaatcac tttcatcttt ctacctcctt gttatatatt
     3841 cagtatatgg gtcagttaaa gaaattccat ccgtcccaag ttggagattt ttctcctact
     3901 ttagaagcga gaatcgagtg tttttttagt aaaaatgatc gttatttgat attattgacg
     3961 tgtggataaa tcagtttttc tcgttttgtc catgtaaaaa atcatgttga tagcaaagga
     4021 tgtgttcaaa atgaatcaag aagaattatt tcaaaaagtc aaggaaatga ttaagaacgg
     4081 caatttcgat ggcgcaaaac gatttatcga agaacataaa gaacaattag gaccttataa
     4141 agagaaagcc caaaatcttt taaaagatgt aaatatcgac agtgtcaaaa acaaatttaa
     4201 aaatctattc aaataagtaa aactttcact tttgtaaaat caaaggagga caatgctgct
     4261 gcatcgtcct cctttggttc ttttctgaat acttcatact aaaataaacg tgtcggatcg
     4321 taatgataag tcacattgcc gttcagtgag gcttttccca ccacatattt acgattctcc
     4381 aacttcagcc acgcttctcg aaaagccatt aattcttctc tttttacatc aatcttttcg
     4441 atttttccat cgatcaaatc ttgaataagt tgttcatatg gattcatttt tttcctccat
     4501 ctgctgtcaa tcaataagtt gtaaaaatag gttcatatgc ttttttggta tctagatctt
     4561 ctctgacttt gactgtcgta taaataattt tttctactgg gatactataa taacttcctt
     4621 caccattatt gaatcggtga acgtctttct ttgctaaaat accatttatt ttattcgtca
     4681 gtacacgttg ttcgacactg ttacttacaa catagtaaat cacccgaata ttattgatca
     4741 aatgcaaatc gatttctaac actacatgat ccatgaaaat cccactcctt ctttcacttt
     4801 gattatagca agtttctcct ctaatttcaa aatcgataca gattttaccg gataaatcca
     4861 caaaactgct tgcgccattt cgcacaaagt gttattttgt atcatggact ttatttatcg
     4921 gaggcaatag tatgatcgcg atcggtcaat ttgtttttta tattcctttt tttattatga
     4981 taagcattct tttctattac ataaaatgga caaaaaagaa gttttcagtt ttactggcct
     5041 ctttacctgc tgtatatttt acttatcaaa ttttttcttt taggcattgg gaaacaacgt
     5101 ctgtattaat aacacacatc atcgaattga cgctttcagt aatttttctt attatttgga
     5161 tctatttttt atataagaat cagaattgaa tccctcgcat gtttaaaaag aaaacgatta
     5221 aagatgcagc aaatttctgc cgatctttaa tcgttttttc tattctgtta taccaaaaaa
     5281 atgtttgaat cggtgaagga tcgttccttg ttttaattct ataaattctt gtgcgatcgg
     5341 gtacttttct tccaaatttt ctttgttctt ttcgatcagg cttttcgctt cttcatattt
     5401 tttatctgca catagtgctt ttacttcctg taatactttt cctgcttcca tttgcctcac
     5461 cctcttgcca ttttattgct ttaagtttta tttaagttat acgcattatt ctagtttcat
     5521 cccaaataac cttttttcat ttttactcct cgaactaatc agttttctaa aggctgattg
     5581 gttcgtcttt tttatttttc ctccatttca ttctgctctt ctgttaattt atctaatgct
     5641 ttgattgatg ctgcggctct tgcataatca tagctgtata attccataaa tttatcaggc
     5701 agaacaaacc acgctggatg ataaccgtta gctgtgacag ggaatccttt atcttgttgg
     5761 ttatctccat cccatctcag agcaacacct ttttttcctt tccactttac taaagcgatc
     5821 gagtatccct tctttgtttg tgtgccgtct tcaataattg ctaaaatttc tattttttct
     5881 tttggcgcac ggacttcttg aggacgaacc attttctatt cctcccttat ctcattactt
     5941 cacgaccaac acactgcaag gggcatgtga tacgacataa gaagcggtcg atccaactaa
     6001 tgcttgttgt atagcacctt ttcctgtcgc tcccataacg atcaaatcaa tattttcttg
     6061 tccagggatg acattggcaa tcaaatttct tggatctcca gtttcaacta ttgcgtggat
     6121 ctcagcaatg ccgtactctt tggcatcatt gatttttttc agcatggcta cttctgttct
     6181 ttgtttttca tctgcataga tttttgaaaa ggcataggca ctatgggtca gctcaacttt
     6241 gttgatgatt gacacgataa ataagtgggc ttcattgtcc tttgctaagt caagggcttc
     6301 agcgaacgct ttgatcgatt gtcttgatcc gtcaactgct accatgatgt tctggtatct
     6361 tttactcatt ggaaccgcct cctttttctt ttattgtaac atctcaagca aaaatacaaa
     6421 aaatatacgc aatatgtaaa taaaatgatg aacaaaagca atttctttca agttatttat
     6481 acatttcttt tctcatcaga acacctgttc taatcccatt atttctgcat tatgtgaaat
     6541 tcctcatcat ttttcacatc gagcgaacat cattctgtta caaaaaacgt ggatttgtca
     6601 taaattgtca tgcttctttt acctatgaaa tattttcacg tgttaaagta ttcctcgttg
     6661 aacacaacaa ataaaacttt ttaacgaata aaggagattt ttcatgacat cacttaaaac
     6721 tttacttttt ggaacaactt tggctgccgg cgctgcattc ttcatgggaa cgactgctca
     6781 tgcggacgaa gcttataccg ttcaatcagg tgatactcta tcaacgattt ctcaaaaata
     6841 cgttggtgat aactccttga tcaatgccat tgcagaatca aattcaattt ctgatattaa
     6901 cctgatctac tcaggacaac aattaacaat cccaacagaa ggctctgcac aagcagctgc
     6961 tgaacctcaa gcagccgttc aagaagcacc agtccaagct gaacctgttc aagctgaaca
     7021 acctgttgta caagaaacgg ttcaaacgga aacacaagca gcgcctgttg ctgaaacaca
     7081 acctgctcct gctgtaacag aaactgctgc tactccagca agtacaagct ctgcaaaaga
     7141 atggatcgca caaaaagaat caagcggttc ttacacagca acgaacggac gttacatcgg
     7201 ccgttaccaa ttagattctt cttacttaaa tggtgactac tctgctgcaa accaagaaag
     7261 agtagcagaa caatatgtta cttcacgtta tggttcttgg gaagcggcga aagcattctg
     7321 ggaagcaaac ggttggtact aagattcatt atttgaacgt ttaaacacat tcaaaatata
     7381 gtaagaggtc atgcgattta ttctttcgca tgacctcttt ttgagtttaa gtttagtctt
     7441 tattccttaa ctatcgtttg ccagtatttc ttgtatcaga atctataaat ctactatttg
     7501 aatcaatcta ttaggtttta aactgtccca ccttcacaca aatttttttt gatttatttg
     7561 aatctggttc cgttgtttaa aatttttcct atattctttt agataatatg ttattttctt
     7621 tctcatcgct ccaggtgtac cttctcttta taaaaaaaca aattatgcgg ctaagaagac
     7681 tcgaacttcc acgaggtcac cctcactagc gcctgaagct agcgcgtctg ccaattccgc
     7741 catagccgca tcacatcttt tagtttagga ttacatgtgt tttttgtcaa gaacatattt
     7801 ttgttctttt tcatttctgt acgccctccc ttatgccatc tacacaaata attcatccga
     7861 gcaactccat catctattga gggttggtga aaaaatctgt tttatccata tgatcgattt
     7921 caatgttaaa cgaacgactc tgggattagt aactatttta tttttttctt catttctttt
     7981 ttcctctttt tctcgattca gctatcttct ttcatttgga tattgattat ttcttctgtt
     8041 gattactatc cttctggaat tctagacgct gaagtaattc acgttttctt tcttctagag
     8101 atcataagta acgaatgaac gaacttcaaa taattttttt attgtaattc atatttgtat
     8161 tgttcgatga ttcttaatac gatatgacac aataaataaa aataggcatg gctatttaca
     8221 acaatagtgc tatagtagaa aacaaatgaa taaggttact taaagaggtg aatacgatga
     8281 aactgatcaa actatgggaa gcagatttaa agaaagctta tcagctacaa actagctttg
     8341 gaccaaatga gagtggattt atcaacaatg cttatggcta tacatttgct gaatttttag
     8401 attatgtaaa aaaatgcgaa aaaacgtctt taggaatcga tcttcctgag agttacgttc
     8461 ccgcaaccgt ctttatttta gaaagtgatg aacatgagta tgtaggaatt tttaatttgc
     8521 gccataaact gaacgaaaca ttaagaaacg gcgctggcca tattggttac ggcatctccc
     8581 ctaattatcg taaaaaagga tatgccacac aaggattagc attagtacta caagaggcaa
     8641 aaaaaatcgg cattgatgag gcacttttgt ctgtaaataa aaaaaatctt ggcagtctta
     8701 aagcacaaca aaaaaatggg gctgcgattt atcgtgaaga tgagaaagaa tactatacga
     8761 aaatcaaact gatgtaaaaa agaaggattc caaaaggaaa gggatttgaa tgtcctttat
     8821 caatagaaca catttattta attaacttat aatatagcag acaaaatttt ataaacaaag
     8881 aaacctttta tctcttttct tctagaaagg ataaaaggct tttttgtttt tcctatttgc
     8941 ttctcatagc tagtcagcca agatttctat cgtatcccca acgcaatccg tgcataacgg
     9001 ctcatcttat ctgtcgtcca tgctggatac cagactagtt tgacttctgc tttcgttact
     9061 tctggcactt cttttaatgc gtcatggata gattctgtta aaatatctgc taatggacag
     9121 cccatagttg ttagagtcat cttaatgact gtttctccat tttcaggatt gaattctata
     9181 tcataaatca agcctaaatt gacgatgtcg attcctaatt caggatcaat caccatttcc
     9241 aaggcagcaa gaatacgttc tttgattagt tctatttctt gtccagaccg ttcttggttt
     9301 gcttcgctca tcgtacgaca ctccttaaca cgatttttat ttatcatact ctttttttag
     9361 ctgcttgtaa atgagaattt agaagtaaga ttctcgttta aatttataaa aatgttctta
     9421 ttagatgtaa tagattaaat ttattctaaa ataaacctga ttcctcattt tttttgtgaa
     9481 cgatttttta tttctggcaa tacgccagtt gttcgataca ttttttgttc gttttcattt
     9541 gcaaaccggc caaatactga atgagttggt aaaatattga taaatgcatc gtcgtcgatt
     9601 tcatgaacga tctgatcaag atcatacatt tcgtagcggg tgatgaccat catgatcatg
     9661 ttcccttgtt tttttgaata gccaccgaca gacggcagta atgtcattcc acgtaccatc
     9721 cgttttgcta gttcttctgt tacctcttct ggcttgacgg taacgatcat tgctgttact
     9781 ttttgatgac tcgtgtggat ggcatcaatt acttgagtca ttgcataaat ggaaataatc
     9841 gtatacaagg cactttccca gttaaagaag aagcctgcga tgactacaat gataccattt
     9901 agaatcagca tgtagtttcc gactgttttt ccagttgttt gtgaaagtac aagagagatg
     9961 atatccatcc caccagttgt aaagcccatt tttaaagata atcctacgcc aagacctaat
    10021 aaaacaccgc ctactagggc attcatcaaa atattatcag tgatctgtcc gactggaagc
    10081 agcatcgtca tcaccgatac acatacaaca ttcacaaaac taaggatcgt tgctcgtttt
    10141 cctactttga gaaaccctaa tacaaacaca gggatattca aaagaaaaat aaatgttcct
    10201 gtgtctatct tgatatgaaa ccattcatat aaaagcgagg aaatgatttg agcgatccca
    10261 ttcatccctg cagaaaagac attggctgga atcaaaaaat aatttaaagc gaccgcagcg
    10321 gtcaatccgg ttatcaaaat aaccactatt ttctttaatc tttcattttt atgatacgca
    10381 gtcataattt tactcacaag cacctctccc ctactttggt attccaaatg taagaataac
    10441 agtttatcta ttaaaataaa atacttcgaa ctcaaatgag aagaatttta gcaaacaggt
    10501 aagaatctgc cctaatccta tgataagata gatgtatggt aaatatttat atgtatatca
    10561 ggagggttcc tatggcaaaa gatgctagct tcgacattgt atcagaagtc aatacagaag
    10621 aagtaaaaaa tgcgattcag caaacaacaa aagaattgtc caatcgcttt gacttcaagg
    10681 attctacagt agaaatcaaa ttagagaaca gcaatctagt tgtattgagc gatgatgatt
    10741 tcaaaatcga acagatcaaa gatgtattat ttggtaagct gatcaaaaga aatgtgccaa
    10801 tcaaaaatat ccatttttcc gaatcagaac acgctctagg tggcaaagct cgacaaactg
    10861 ccgatttggt caacggaatc gatcgtgaaa acgcgaagaa aatcactaca gctatcaaaa
    10921 atgaaaaact gaaagtaaaa gcacaaatcc aagaagatca gattagagtg acaggtaaga
    10981 accgtgatga tctacaagct gtgattgctt tattacgtaa acttgactta ccaatcgagt
    11041 tgcaattcac taactatcgt taatcaaaaa acgaggctga cgtctgtcat tcctcgtttt
    11101 tttgatttac tgcttctttt gataaccaaa aacgctgtat tccttcttcc aattccccaa
    11161 cacttcgtac ttctcttgct gtttgccaat gttgtgggaa taaacgtcga taagaaaaac
    11221 tagaaaatcg ttgatctgcc aatacaacga ttccttgatc cgccatgcca cgaatcactc
    11281 gaccaccagc ttgcaagact ttgttcattc caggaagctg ataagcgtaa gcaaatccaa
    11341 ggtgttcctt ctcatcataa tatgatttga tcagttcctg ctcatgattc atctgcggca
    11401 aacctacacc tacgatcata ctgccgatca atcggtcccc tctcaaatcg attccttctg
    11461 aaaaaatccc gccaagtacg caaaatccaa tcaatgtttc agcgggattt gtttgaaatt
    11521 ttgttaaaaa tcgttcgcgt tcttcctcat tcatgtccgt ttcttggatt agcacattgc
    11581 tgtcgggata ttgctgtctg aatgcttcca ccgcaagatc taaatattga taagaaggaa
    11641 agaaaacgaa ataattcccg acctttccat cagcaaaagc atgaatcgtc tctactaatt
    11701 ttgccagact gtgttccctt tttcgataag tcgtctctag gtaatttgcg atgaaaactt
    11761 gctggtttcc ttctgggaaa ggactcggca gtttgtaagc cagacttttt tgtcccccca
    11821 gtgtttcctg ataataagag agtggtgaaa aactagcaga aaaaagaata ctgctttttc
    11881 ctttatccaa cgactgctca agaaacaagc ttggatcaat acaaaactct tttactatca
    11941 gatcgcgata cgttttttcg actgttgttt cgtagtgatc atcataaaat tcactgacct
    12001 tcaaaaaatg aagcaaatca aaataataag gaagcaattg ttcttgtgat gggtgctcag
    12061 gaaattctgc cagccattct ttaatttttt cgcttaattg ataacctgcc tttactaaag
    12121 attcagccgg tgctttttga tgatgataat cccaatgatc ttcttttgct atttctcgga
    12181 tcgaatcaaa ttcttttaat agtttgttga aacggcgatg aagttttttg aattcctttg
    12241 gtatagcttc cttacttcgt ttcgtttttt cataagatag ttctgccgaa tacatctctc
    12301 gggaacgatt caccaaatta tgtgcttcgt caatcaaaaa cagatagtcc tcgttttttt
    12361 catcttcaaa aaaacgtctt aaataaacag ttggatcaaa taaataattg taatccccaa
    12421 tgattacgtc acaccagata ctgacatcta atgatagttc gaaaggacaa agtgtatgct
    12481 ttcttgcata agtttcgatg acttcacgag tgatctgatt ctcatgattc aaaagatccc
    12541 ataaaccttc attaatccga ttgtaataac cattggcata tgggcactga tctggattac
    12601 aggtcgtctc atctaaaaag cagatcttgt ctttcgccgt caaagtgaca ctttttactt
    12661 cagctcctgt atcttttaat gctgtcatag catcttcagc tacttgtcta gttattgttt
    12721 tagccgttag ataaaagatc ttttctccct cttcttcccc tacagctttc aaagcgggga
    12781 ataacgtaga gattgttttt cctgttccgg taggtgcttc tacaaacaat ttctgtttcg
    12841 tacggatcgt tttataagca gctaccgcta gttcacgctg acctttccta tactcatcat
    12901 gggggaattt caaagccatc aaagaggcat tgcgtacagt gcgccagttt tcttgaaaaa
    12961 caagccattc tttataatct tcagtcagct ttttaaaaaa agtttctaac tgctctcttg
    13021 tctgatgttc aacctttctc gtgatcactt cttctgccgt ctggaagtaa gtcaattgca
    13081 gatttatttc ttgtaaatca tgttcttgac tatagatata ggcatacacc cttgcttgat
    13141 gaaagaaaag ttccacttgt tctggttcta aatcttcaaa tcgtggttcg gaggttttga
    13201 tttcatctat ataataaata ccgtctcttt tgaaaatacc gtctgcacgg ccttcaacga
    13261 tcaggtaata ttcatctaat tgaactgttg tttttaaaaa gacttctttt tcatagtttt
    13321 ctcccgcatc tttttggagc ttgcggtgga ttctcgctcc ttctaatgca gtatggttac
    13381 tttttttacg attgtcgata cttccacgtc gcaaaataaa ttctactaag tgccgcacag
    13441 atatttttga ctgtttgatc atttccacca cctctttctc tctaagatta cctgaaaaaa
    13501 cctagtttag caaaaagagc tctctttcca aacataaaaa aacaccctgc tgattaggca
    13561 gggatatagg atatagaatt tggatttttg gatgaatcta atgtaacaag gacatattaa
    13621 aatgctatga aaacttactt tccttctctt tagaatatcc aaatactttt catatagtct
    13681 gataattcag ataagtaaag agaaataagt acctgttttt gtttcctagc aatgaatgcg
    13741 ttatactaat ggatgaaaga agttttggct atcaaggagg aatgcacatg catatcgaaa
    13801 gattaaaaac aggaatgatt gaagaaaact gttatttggt ctacaacgaa gaagcacttt
    13861 tgatcattga ccctggagaa gatgcggaac gaatcaaaca agaaatcaaa aaaacaaacc
    13921 aacagccggt agcgattttg cttacccata ctcactacga tcatatcggt gcagtggaac
    13981 ctttacgcgc gtattatcaa atcccagttt atgtcagccc actagagcaa aaatggctag
    14041 gtgatccgat tttgaatctt tctggactcg gtagacatga tgatattgct gatgttattg
    14101 tctcgcctgc agaatatgaa tttgaaatga aaacttatac tttaggaaac atgtcttttc
    14161 gcgtcgtccc taccccaggg cattctatag gaagcgtcag ctttattttt gatgattttg
    14221 tcgtttctgg cgacgccctg tttagaggaa gtatcggtag aaccgatctg tatacaggga
    14281 atttagaaca attacttcat agtattcgga cccagctgtt tgtattacct gatgaatttg
    14341 cagtctatcc aggacatggc gatgcaacga cgattgaaca ggaaaaaagg acaaatcctt
    14401 ttttcaatcg ttaaaacgac ttaggaggag aataaaatga cagaaactac actttatatc
    14461 gtacgacacg gaaaaacgat gttcaataca attgaacgag tacaaggctg gtgtgataca
    14521 cccctcacaa agcaaggaca agaaggcatt cattatttag gtcgcgggtt aaaaaatgtc
    14581 gacttttcat ttgcttattc tagtgacagt ggtcgtgcaa tcgaaactgc ccgtattatt
    14641 ctaggagaac attctaaggg aaaagaaatt ccttatttta ttgaaaaaag gattagagaa
    14701 tggtgtttcg ggtctcttga aggtggatat gacatggaaa tgtggggtgt gatccctcgt
    14761 gtattgaatt ttaaaacata tgatgaaatg tttaccacag aagtttcttt tgaacaaatt
    14821 gctaatgctg tatacaactt agataccgct gggtgggcgg aaacttatga aacattgcga
    14881 gaccgtgtat ggacaggatt tgaagatatc gcccatcact gcgaaaaaaa tggtggtgga
    14941 aatgttttag tcgtatctca tggtttgacg atcgcctttt tgcttaatct gatcgatcct
    15001 aaacagcctg ttcgtgctgg gcttttaaat ggcagcgtaa caaaggtcac ttatgcaaac
    15061 ggtcgatttt ctatccacgg catcaatgat acgtcttata tcgataacgg gaaaacacat
    15121 agtcgcgact cgtttctata atggggatat tttaattttt accagagaat cataaaaggc
    15181 aaagtctgaa cgagggcttt gtcttctttt ttctcttcgt gtacttatct gctttatttc
    15241 tcaagttttt cttaatatta aaaaacagat tgggtttctc aaaaaataac ggtatactat
    15301 aagatgtatc ttaattggga aggaaggaat ttatgttttt tgcaaatatt atcaaagcag
    15361 tgattctcgg tattattgaa ggaataacgg aatggctgcc catcagtagt actggtcatt
    15421 tgatcctagc agatgaattc atcaaactgg atgccagttc acaatttatg tcgatgttca
    15481 atgtcgttat ccaattaggg gcgatcttgg ctgtcgtcgt tctttatttt cacaagttaa
    15541 acccattttc tccaagtaaa tcaacggtag aaaaaaaaga tacgtggaca ctttggttta
    15601 aagtgatcgt tgcttgcata ccagccatca tcattggttt gcctttggat gattggttag
    15661 aagcaaattt ccacaagttc ttaccagttg ccattgtttt gatcgtttac ggtattgcct
    15721 ttattattgt cgaaaaacgc aacaaaaaca aaacacctcg ttggacttct ctgaatgact
    15781 ttacgtacca agcagctctt cttgttgggt tattccaagt cttggcactt gttcccggaa
    15841 catcccgttc tggcgcaacg atcctaggat ctatcttgat tggtgcttcg cgttttgtcg
    15901 ctacagagtt ttcatttttc ttagggattc ctgtcatgtt tggcgcaagc ggcttgaaga
    15961 tcgtcaaata tttagctgaa gggaattcat tcaaaatgga agaaactgtt gttcttcttg
    16021 taggaacatt cgtatctttt gtggtttccg tcttagctat caaattctta ctaagctatt
    16081 tgaagaaaaa cgactttacc gcttttggct ggtatcgtat tatccttggt atcattttga
    16141 ttggttattg gtttattgca atgtagaaaa aaccagaaca cttctctt
//
LOCUS       DACDTH010000065        15891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDTH010000065 DACDTH010000000
VERSION     DACDTH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.065"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(647..1013)
                     /gene="ssrA"
                     /locus_tag="IWK54_002228"
     tmRNA           complement(647..1013)
                     /gene="ssrA"
                     /locus_tag="IWK54_002228"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1184..1957)
                     /locus_tag="IWK54_002229"
     CDS             complement(1184..1957)
                     /locus_tag="IWK54_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9594329.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2101..2451
                     /locus_tag="IWK54_002230"
     CDS             2101..2451
                     /locus_tag="IWK54_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9594330.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2432..3148
                     /locus_tag="IWK54_002231"
     CDS             2432..3148
                     /locus_tag="IWK54_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9594331.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3148..4596
                     /locus_tag="IWK54_002232"
     CDS             3148..4596
                     /locus_tag="IWK54_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9594332.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4666..5175)
                     /locus_tag="IWK54_002233"
     CDS             complement(4666..5175)
                     /locus_tag="IWK54_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594333.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5165..5890)
                     /locus_tag="IWK54_002234"
     CDS             complement(5165..5890)
                     /locus_tag="IWK54_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594334.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6113..8233)
                     /locus_tag="IWK54_002235"
     CDS             complement(6113..8233)
                     /locus_tag="IWK54_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9594335.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8463..9641
                     /locus_tag="IWK54_002236"
     CDS             8463..9641
                     /locus_tag="IWK54_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9594336.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9657..10415
                     /gene="aroD"
                     /locus_tag="IWK54_002237"
     CDS             9657..10415
                     /gene="aroD"
                     /locus_tag="IWK54_002237"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9594337.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10438..10923
                     /gene="ybaK"
                     /locus_tag="IWK54_002238"
     CDS             10438..10923
                     /gene="ybaK"
                     /locus_tag="IWK54_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9594338.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10994..12247)
                     /locus_tag="IWK54_002239"
     CDS             complement(10994..12247)
                     /locus_tag="IWK54_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9594339.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12365..13714
                     /gene="gdhA"
                     /locus_tag="IWK54_002240"
     CDS             12365..13714
                     /gene="gdhA"
                     /locus_tag="IWK54_002240"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9594340.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13826..15172
                     /locus_tag="IWK54_002241"
     CDS             13826..15172
                     /locus_tag="IWK54_002241"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9594341.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15480..15866)
                     /locus_tag="IWK54_002242"
     CDS             complement(15480..15866)
                     /locus_tag="IWK54_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9594342.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5189 a         2822 c         3187 g         4693 t
ORIGIN      
        1 aaacgaagta cgtcttctga aaaaaagtgg gtatattcac agactatttt ttacggaggg
       61 gttatttcaa tcagtttact ttcttcaaca ctttattctt ttaatgttat agacgtttcc
      121 atgtcaaatt ttatatctat tattggaatc cttatttctg caattattac acaactactt
      181 cttgtgtttg aagagaagtc aaaaaataat taaagcaatt atgcctttta gtacaggaat
      241 tattacttct tacaaatgta tagcgaatgt gaagcctgct gttttaattg ttctagaaaa
      301 acttttgagt gaacaattat tttatgttag gatgaattaa tttctctaga gtttataatt
      361 aaaaaactta tttgaaggaa catgcttaaa ccataattgt gtttttcctt ccatcagcat
      421 aaaagttcac ctcaaaaaga gtgtacgaac aaaagttctt tttgtaaacc ttaaaataga
      481 ttttcctgaa tacgtattgg ggatttctaa atgtttgaat gtttttttga atgcaaaatc
      541 aatgcaaaaa attttgtatt tctctacttt tctctccttg accaaaataa aaaaacgttg
      601 atttaacaac gtttttcata catgtttatc tgtctcaacc atacatatat ggagacggcg
      661 ggagtcgaac ccgcgtccaa acacatcgcc acttaaaaat ctacgttcat agtcaactca
      721 tttaaggatt cactttacag cttgccgagt gacaggcatt ctgtatcgct agtctgatgt
      781 tctctttttc aacttacaga cggaaagttg aaacgtatcc cactaaagtt aggaccctta
      841 ctcgagcaca tgggcgatgc cgagaggatc tacgctaact gtttttaggc agctaaagcg
      901 taagagttgt tttcgttttt agcagttata tttaactgta acgtttttac gtagacgtaa
      961 cctacgaaac gcaattcaag ctcgactgtg cctgtcgaat ccgtaacgtc cccgttagct
     1021 aacaagggtt acgtgtattt ttcatacaaa attagtatag catatattgg gataaattca
     1081 ataaaaaagc gaatcatatc ccattattta tataataact gcccctttct taagaaaaaa
     1141 taagcgattt tcttaagaaa agaggtagct tcaaaaaaag atcttaaatc agatcaaaat
     1201 acttcaatgc gtggacaatt ccgccttctg tatttttctt tgtgataaag gtagaaagtt
     1261 cttttaatcc gtcataggcg ttgcccatag cgattttgtt gtcgcaagct tccaacaagg
     1321 caaaatcatt cggaccatcg ccaaatccaa aagtaggaac tttttccagc tgaagttctt
     1381 tttgcagacg tttgacacct gccccttttg agattccttt cttcactgta tcgatcgagt
     1441 aaggaccatt ccgataaaaa gttagctcag gaaaatgttc gtgatagtat tcgtcacctg
     1501 attgagataa gatcagcaac atatttactg tgttttcttt aaagcctaaa ggatcaattt
     1561 ccggaacatc tgaatgaatg aagtcgtaag cattgatcat tgtttggcta tgaccggtac
     1621 accagatttt ctgatcgtta tagtaagaaa gttcatgacc gttcaactgt acatgatcat
     1681 acattttttg acattcttct gaagaaaatg tttgagaata gatttcttcg ccgtctactc
     1741 gaatgaaaga accattcatg gtgaccgcag aagtaatgcc cgcatcttcc atgatagagt
     1801 ggatttctgc ttctgtacgt cctgtagcga tgacggggag aacattgttt tcttttaatg
     1861 cgttcatagc ttttgccact tcgggtgtga tttttgattt cgcatctagt aatgttccat
     1921 caagatcaaa gaaagtgatt gcacgataat ttgacacaaa taaaactcct tttttattgg
     1981 aataagttta tcttaacaaa gaatgaacaa aagacaaacc tttcgtgaag aattcctgaa
     2041 catattggtt cagtcaacaa tttagagtat actataagaa gagaatttag gagggaaaga
     2101 atgatttttt tcagtattgg tatcttattc ttaataattg gcagtctttt tcggattctg
     2161 ccctcaaaag gaaatctgcc tttttatggg tatcattcgc ctcttgccgc aaaaacagac
     2221 gctcattggc ggctggctca aaagacaagc ggaaactggt tttttcttat gggcttactg
     2281 atggcattga tcggctatta tttaaagaca tctgggcata cgaattattt tctgattgaa
     2341 atgcttctgc tagtatttcc aattatgcct atttttatta tgacagaaaa gaaattacag
     2401 aaatatgacc tggaaactgg agggaacgat aatgaatatt ttaatgattg aagataatca
     2461 atctgtttct gaaatgatgc agatgttttt cttaaatgaa ggttgggaag caactttcaa
     2521 gtatgatgga aaagaagggc tcgatgcctt tttagaaaac ccacaacatt gggatatgat
     2581 tacactagac ctgaatttgc ccacactgga cggtatgcta gtaggaagag agatccgcaa
     2641 agtttcaagt actgtgccga ttatcatgct gacagctaga gattctgaaa gcgatcaggt
     2701 gatcggctta gagatggggg cagatgatta tgtcacaaaa cctttcagtc ctctgacgct
     2761 tattgctcga atgaaagctt tgcatcgccg tagtgagttg gcagaagcaa gagaagaagc
     2821 agcagtttca gatgatcatt ttgatatcga gactagtcat ttcaagatga atacgaaaac
     2881 acgagaagcg tatctggaca accggccaat tgaaggactg acgcctaaag agttcgatct
     2941 attgtttact ttagcaaaaa aaccacgtca agtattctct cgggagcagt tgcttgaatt
     3001 agtctgggat tatcagtact ttggtgatga acggacagtg gacgcccata tcaagaaatt
     3061 acgacaaaaa atcgaaaagg tcggaccaca agtcatccaa actgtttggg gagtcggcta
     3121 caaattcgat gattctggtg ttgcctaatg cgttatttgt atcagcaact cttagctttt
     3181 tgcggaatgg tcgcgttgat cttattgatc gttggagttt cttttacaca actgacaaaa
     3241 caaacgattg aagaaaacaa ttaccagcaa ctgtttggat atgctgaatc agttgaaaag
     3301 acaacgcaaa cctatgccga tacgctcccg cagtttagtc aagatcaggc gtttcaaaat
     3361 gcattgctgc tgacagagca agtactgaca gaacaaaatg tgaactttat atttatcgat
     3421 aaaaacgaac gtgtcattta tccgactaat acagctttac tgaattttac cttaacagct
     3481 gatcaatgga aaaatctgag gaatggacgt caagaaaaat tcacatcgaa taaaaatatt
     3541 ttaggacaag cacaggcgac ctcttacgca cttgtgccat ttaatttgaa tcatgaattc
     3601 tatggagcac ttgtagttag tcaacctgcg cgaaatatcg ataacagcgt tcgtccaatt
     3661 acactgaacc tgttcaaagg atttattttt tccagtatcg ttgcagttat tgccagctat
     3721 gcttttgcag cattccaagt taagcgtatc aatcggttaa ggaatgcgac gaaagaagtg
     3781 acgaatggta attttgatgt acagctcccc gtacatgaca aagatgaatt tgatgagtta
     3841 gcagatgatt tcaataaaat gacgaattca ttgaaagaat ctcaagcaga aattgaagaa
     3901 caagaaaatc gtcgaagaca atttatggca gatgcttctc acgagatgag aacacctctt
     3961 acgacaatca acggacttct ggaagggtta gaatacaatg caatccctga aaatcagcga
     4021 gaaaacgcga ttaaattgat gaaaaatgaa acagagcgtc tgatccgttt ggtgaatgaa
     4081 aacttggatt atgaaaaaat ccgtacgaat caaatcagta tggtcattaa aaaattcaat
     4141 ggaacagaaa cattgaaaaa tattgttgca cagttagaag ctaaagcaga agcagcagga
     4201 gatacattga cattgaaagc cgttgatgat attgatgttt acgctgatta tgaccgattt
     4261 gtccaaatca tggtcaatat tattcaaaac gcgatacagt ttactgaaaa cggccaaatc
     4321 acgattactt tagaaaaagg ctatcttgaa acgatcatca cgattgaaga tacgggaatt
     4381 ggaatgtctg aacaacaaat gaaaagtatc tgggatcgtt attataaagt agatccttca
     4441 agaaaaaata caaaatatgg tgaatcagga ttaggtttac caatcgtgca gcaacttgta
     4501 cgcttgcata aaggaaaatt agaagtagaa agcgaactag gaaaaggaac aaaattcgct
     4561 gttcgtttgc ctgacgtaga aatcaaaaaa gagtaaaaaa caagcggtat aaaagatcat
     4621 gaatcgatct tctataccgc ttgtttttac tataaaatga ttgatttagc ccgcgggctc
     4681 taaacgatca atataaggac tttgagcaat agtagcatca tgagtaaaga gtaagatacc
     4741 ttgattttct tcatgagcta aataagaaag caagtggagt agctcttgga tctccaatac
     4801 actaagttca tcaaatgtat cttcaataac aatattgttt cgcttggaaa gcaaggcatg
     4861 aagtaattgc agctttactt tttcaaaaga cgtcaactca gctgccgcac aattaagcaa
     4921 ggaaggagag atatcaagtt tttcttgata ggccaaacga tcttgtttga tttctttttc
     4981 cggtacatcc aacaggacat ggtctttaag attcagataa ggaaacatcg gccaattttt
     5041 ttggatgatc gcaaaattat ccagttttgc aacaagaacc tcttttttca agtgctccaa
     5101 tattttatgc tgtttttcga gaggacacaa aataacggtc aagtgttcag catgaatcaa
     5161 atattcaggt ttcattacgt ccaggcctcc tactttgcaa atgatggata aaaaaagtga
     5221 aagggaatag actaagaatg agacagcatc cactgagccc aataagagtt tgggcaaatc
     5281 ccttaagtat atctgtgaaa aaaagatcat tttggaaatc gatggtaaaa aaagtctgtt
     5341 gatccatcgg aattgtaagt ccgcttaccg aagtcaagat atcacggata tctggcagtt
     5401 cagtagtctt taagacaaga aaattgatcc tttgaagaag cgcttcataa actgactgga
     5461 agcttacgat ccacaataaa accaagatac aacaaagaaa gagaggaaag aacagctgcc
     5521 agaaaataaa aaggaataat ttggttttag aaagaccagt caggcgccaa ttgatcaatt
     5581 cctgcttgtt ttgcctgact gaatagtaaa agaaaaagat aaaaaggaag gcaaatagaa
     5641 aaaataaact gaatatgcct tttatataaa atcggctagc taattctaaa gccggaagaa
     5701 gctctgtaga tgcagaagcg ccaataaatg aaagccgatc ttggacttga ttggctaaag
     5761 ccgattgcaa atggtaaaga gtcaggcatg ctatcaataa aaaagcagaa aagcttgaaa
     5821 ataaacaata gagtattgtc tgctttttgt aataaattaa tcgagcagta gcatagcgga
     5881 ttattttcat acttttcacc tctataaaaa gtatagaaaa tgaatatgaa taaagtatga
     5941 aattccttta gtgaaatcac gaattaaaac cagaaaatat tttgtaaaaa gcaagaggtt
     6001 gtgaagaagc tgccctattc tattaccgta gatgcaagct tttgaacact gtttattcga
     6061 aaagaggagc agcgacaata attttgtcgc tgctcctttt aaatttattt tgtcattcgg
     6121 aagaacttgt ggtcgttccg tgaagttctt ggtacgtttg cgttttataa agatcaacag
     6181 ttgattgtcc gttgtgttca ctaaagacac tagttgactt gtcaccaaga gatttttcag
     6241 cttcttttaa tttctctaag ccattcttgt agtcataatc actggtatca attgggtcta
     6301 atccactatt cgtatagaac cgaagaagat caccattatt gatttggtca gaaacagcta
     6361 actgtttatc aactttttct ttcagtgaat caatttcatt ctgtacttcc tcagtaggtt
     6421 cagtaatagc ttcaccagtt tcgttactat agatcgtacc accgtagtaa gtatattttg
     6481 gtgtaacaaa atcaccatca cggaaagaaa caagctgttc atgatctttc gagaatagat
     6541 cttgtcctaa ctgaatataa ttttttgtat ctacacctag taagtgaagt aatgtaggca
     6601 aggcatcaac ttgtccgccg taagtgtgat tgattccgcc ctgtgtttgt ccagggatat
     6661 ggatcatata agggacacgc tgcatttgtg cattgtcgta gtttgtccaa gtagaacttg
     6721 tttttccaac taattcagct agatttttat ttcttgaatt cgagacaccg taatggtcac
     6781 cgtaaaggac gatgactgaa ttatcataca agccgctttc tttcaaataa ttgaagaatt
     6841 cttgtacggc ggtatctaaa taattggctg tcgcaaagta accgttgatc gtttcatcat
     6901 ttgtattggc taacgggaat cctgcttcgt cattggtgaa ttttgaataa ggataatggt
     6961 tagatacagc aatgaatttt gaataaaatg gttgttgcag acgttccaag tattgtacgg
     7021 attgttggaa aaatggttta tcatgcaacc cgtattggaa cgaattgtca tcattaacat
     7081 cataataaga agaatcaaag aaataatcat atcctaaatt cttgtatgtc tcgtttcgat
     7141 tccagaatgt accagcgttc ccatggaata ccgcactagt gtaaccttgt gtttgactta
     7201 aaatatcagg tgcagcttgg aaagtatttt ttccgcctaa ttgtgtgaac aaggagcctt
     7261 gattcaagcc aaacaatgaa ttttccatca gcgtttccgc atcgcttgtt tttcctgctt
     7321 ttacttgatg gaaaaagttg tcaaaactga acgtgctgtt gctgtggtaa agactattga
     7381 tgaaaggtgt aacttcatgc tggagaccat tctcatctgt caatttataa tcgatcagga
     7441 attgctggaa gctttctaaa tggatataaa tcacattttt tcctttagcg agcccgaaca
     7501 aatcgtcatt tggtgctgca taatgttctt ttacataatt ttctacttct ttcatatcat
     7561 tgggacttgc ttctgcccgc acttggtttg tctggtaagt ttgaacaccg tcataaacca
     7621 tgaaagcatt caagccaaga tatttgacca aataatcacg agagaatgtt cgagtcagca
     7681 attctggacg atcagcttcc gccataaaaa gatttcctga gaaaatcata actgctaatg
     7741 tagagatcgc aaaagccatt cttgctcgga tcgggcgagg gtccaacttg attttttttg
     7801 caatcagcag aacagtcaag ataataaaat cgatccaata gatcacatca tatgggcgaa
     7861 acagtcgaat ggcactttct cccagaccac tcgctacttt cccagcacct aaaatcgtgt
     7921 tgatcgtaat gaagtccgtg aattctcgat aataagtgac attcgaaaaa acaagcaatg
     7981 tcaaaagaaa ataaatgacc atcatcgtga tataagcaac tctagggcgt ctgatataaa
     8041 gagcaattcc aagtaataac aatgtagttg caatagggtt gataaacaag ataaagtatt
     8101 gaatgccact ttcgattccg agatggaaat caactaaata ggcaaaaatc gttttcaacc
     8161 aaagtaaaac gactagtaaa gaaaaaaatc cgagcctctt attcaaaaag gcaggtgttt
     8221 ttatacattt caagataatc ttcctttctg cacttcttaa tcacagaagt gtcatgaaac
     8281 catgttacac agctcactta ttctcattta ttttactagt atgacaatat agtgtcaatt
     8341 gaaaccagaa gtttatgctg tttcattaag gttttcttta agcttcatga aaaaatgagc
     8401 agtaagacaa gtcctatgtt ttttgttata cttgggaaga gaaagctaaa gcaggtgaag
     8461 gaatgaaagt aaaagtaaaa aaaagagcaa gcaatcgatt gaaaaaagga tatccactga
     8521 tccaaaaaga agaccttgct catccccaaa aagaaaccac gcaatgggtg gaattagttg
     8581 attcgcaaaa ccaatttatc gcaaaaggat atctaggaaa gcagaataag ggaatcggtt
     8641 gggtattgag ccagcagaac atcccatttg atcaatcttt ttttgaagaa ttttttgtga
     8701 agtcaaaaga aaaaagaact ttcttttttt cagatgattc tactaccgct tttcgtatat
     8761 tcaatggaga aggagacgga atcggcggtc tgattgttga ttattatgca ggttatgctg
     8821 ttttttcttg gtataacgaa acgttatatg cacatcgtga attattgatt caagcttttc
     8881 gtgaaatcta cccggaaatc gtaggaggct atgagaaaat tcgatttgaa tcagtaaatc
     8941 tgcctgagag ccagttcatt tacggaaaag aggcaccgga acccttgatc gtaaaagaaa
     9001 acggagtaaa atttgccgtt tatttaaatg aaggattaat gactgggatc tttttagacc
     9061 aaaaagaagt acggggatca ttagcagaag gtttcgcttc tgggaaaaca gttttgaaca
     9121 tgtttagtta taccggcgcc ttttctgttg ctgccgcaat gggaggggca atcgaaacaa
     9181 caagtgtcga tctagcaaaa cgcagtttaa aaaaaacaca ggaacagttt gaagtgaatg
     9241 gtttggatgt cgatcagcaa aaaatcattg ttatggacgt attcgagtat ttcaaatacg
     9301 ccaaaagaaa acaattttta tatgacatga ttgtattgga tccaccaagt tttgccagaa
     9361 ataagaaaaa agtctttcgc gtagcaaaag attatgggga actggtcaag gatagtctgg
     9421 atattttagc agaagacggg atacttattg cgtctacaaa tgctgcgaat gtctcgatag
     9481 aacaattcca aacaatgatt gaagaggaat tccatgccaa aaatgtttct tttgaacaac
     9541 ttgctttata tcgtctgcca aaagatttcc agacgacgga taccttttta gaaggcaatt
     9601 atctgaaagt gtttgtttat caaattaaaa ataaccatta gaaggagcga tgcagaatgg
     9661 tttgggtaaa gggattgaat ataggaagcg gtagaccaaa gatttgtgcg cctcttatcg
     9721 gggccgtccg agaggaagta ttacgtgaag cagttcttgc acgtgaagca ggggccgatt
     9781 tagtcgaatg gcgagtcgat cattatcgtg aagttttttc ttttgatgaa gtagcagaaa
     9841 cattggttct tatccatgag atgcttgatg gactgccgtt attatttacg tttcgtacct
     9901 tagctgaagg cggagaacaa gacatatcga tccgtcagta tcgcgacctt tatgaacatg
     9961 tgatacacac aggattagta gatatggtcg atatcgagtt gttcaaagta gaaagtctag
    10021 gaaaaagact gatagaggaa atcaagtctt taaacattcc tttaattatc agtagtcatg
    10081 attttaaaga aacaccagca gaccctgtct tgctttaccg attgaatgtt atggaacatt
    10141 ttggagccga tatcggaaaa ctggcagtaa ctcccaataa tgaacgagat gttttgcggt
    10201 tgatggagtt gactagacgg gcaaatgctt ttgtctccat gcctattatt acgatgtcca
    10261 tgggaaatct tggtaaaatt tctcgtctgg ctggcggaac gacgggttcc gttatgacat
    10321 ttggttcctt aagcagcgat tctacttctg ctccaggaca attaccggtc aaagcattga
    10381 agcaaattat tcaattatta aatgaaccgt tgtagaacaa gtttgaggtg atattttttg
    10441 gcaaagaaaa aaatagtaaa aacgaatgca atccgtatgg tcgaacaaaa aaagattccc
    10501 tatacagagc acgaatatga gtgggacgaa agccacctaa gtgcgtcatc ggtcgctgaa
    10561 cagcttccag aaagccaatc tcgtatattt aaaacactgg ttgctgtggg caatgtgacg
    10621 gggcctcttg ttgctgtgat accgggagaa gccgaattga atctaaaaaa attagcaaaa
    10681 gtttcaggga ataaaaaagt agaaatgctc catttaaaag atttggaagc aacgaccgga
    10741 tacatacgtg gtggctgctc gcctataggt atgaaaaaac tatttcctac ttatttagat
    10801 caaattgcag aaagctatga acaaatcatt gtctcggctg gcagaagagg gctacagatg
    10861 gagttagcac ctcaagatat ctgtgcatta acgagcggac aatttgcaga tataaaacaa
    10921 taaatcatac gtataaaaga aagaaggagg ccgggatgtt gtttgtccag tctccttctt
    10981 tcttttttat acattatgct ctttgcccgg cattcattcc tgctacatgc cctgtagcga
    11041 aagccgctgt gatattaaag ccgccagtat aaccattgac atccagtaat tctcccgcaa
    11101 aatacagacc atcaatgatt ttactctcca atgttttagg ataaatttcc tttagagaaa
    11161 cccctccgcc agttacaaat gatttctcaa taggaaaagt tttgttgatt gatagcttaa
    11221 actctttgca tagttgaaca aaatcttgaa tttgtttttg agaggcttgt tgtcctgtca
    11281 gatgatccat ttcaagccgc tcaagaaaga aaactaacaa tcgctcaggc aatataccat
    11341 gccaagcatt gaccaagttc tttttggttg ctttactttt ttctgtcaat atatgaataa
    11401 gctcttgttt ggtttgtgta gggaaacaat ccagagaaag tgtcactggt tctcccgttt
    11461 tctctaattc tttattgaca aaacttgaac aacgcagtgc agccggacca gaaataccga
    11521 aatgggtgaa aagtaaatcc atcgtatgtt cagtcaatac acgacctttt ttatttaaaa
    11581 cgcgtaaagt gatgtcttgt aaagaaagcc cttgaagtgt tttctcctga atatacggtt
    11641 catccgatat caagggggac tctgtcgcat aaagaggcgt caccgtgtga cccactcgct
    11701 tcacgatctt gtacccatct ccagtagaac cagtagatgg ataagtccgt ccaccagttg
    11761 ttaagataat ggcaggagca tggaactctt cgaattccgt gcgtataccg taaattttat
    11821 gttctttgtg gatcaatttc tctactcgag tagagaatag aagcgtcaca tctagttctt
    11881 tcagacgatt gagcaatgct tcgataatcg tttttgattt atttgttaca ggaaacatcc
    11941 gtccatgatc ctcttctttt aaatgtacgc cttgtgattc aaagaagttc atgatatcga
    12001 aattattcca ttgcgcaaac gtactatata aaaactttcc atttccagga atatgtgcaa
    12061 tcaaatcatc aacaggacga ttgttcgtaa cattgcaccg accaccacct gtcatcagta
    12121 gtttttttcc tgcttttttg tttttttcga tcaagagtac gcgtgcgcct tgttcagctg
    12181 cactgatcgc tgccatcatc ccgcttgttc cagcgccgac tacgatcaca tcatacagtt
    12241 tttccattcc ctcacctcaa ggaaagtgta gcatatctga agaaagaaaa cagaaaaaaa
    12301 atgttttgag ccaaaagaga aaatgagcta aaatgaggga agataagcaa aggagcgtgg
    12361 aacgatgaca aacgcaacag aatatgttca agcaattcaa gaaaagctac atcaaaagga
    12421 tcaagggcaa gaagaatttt tacaagcaat cgatgaattc atgcccacgg ttatgtcttt
    12481 tttggacact catcccgaat atatcgaaaa gaatatctta gaacttttaa cggaaccaga
    12541 aagagttatt caatttcgag tgccttggca agatgatgaa ggaaactggc gagtaaatag
    12601 agggtaccgt atccaataca actcagctat cggcccttat aaaggtggat tgcgcttcca
    12661 tcctagtgtc aatctaagta tcttgaaatt tttggctttt gaacagattt tcaagaatag
    12721 tttaaccggt ctgccaatcg gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa
    12781 atctgataat gagatcatgc gtttttgtca aagtttcatg ctggaattgt caaaacatat
    12841 cggcccatcg attgatgttc ctgcgggaga tatcggtgta ggagctagag aaatcggcta
    12901 tttgttcggc gaatataaac gattgaaaaa atatgatact ggtgtgttga caggtaaacc
    12961 tttggatttt tggggaagta agatacgtac ggaagctact ggttatggat tagtgtatta
    13021 tgtcaaacat ctattgaacg aagaaaaaga ttcatttaat caaaaaacag tttttgtttc
    13081 cggaagcggg aatgtagcca tttatgcaat cgaaaaagtg caagagctag gcgggaaagt
    13141 tgttacatgt tcagattcta gcgggtacat ctatgatcca gaaggaatag atgtgaaact
    13201 attgaaagaa gtcaaagaaa tcaatagaca acgtttgacg gaatacgcaa accaacgtcc
    13261 ttctgctatt tatcatgctg gagaatctgt ttggacattg aaagaaaaag cggatatcgc
    13321 tttgccatgt gctactcaaa atgaaataga cgaaagcttg gccgaaatct tagtagaaaa
    13381 tgggatcaaa atcgtagcag aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt
    13441 tcttcatgaa gcaggcgtgt ggtattgtcc aggaaaagct gctaatgctg gaggagtagc
    13501 agtatctgct ttagaaatga gtcagaacgt ccagcgattg acttgggaaa gagagcaagt
    13561 ggatgagcag ctggatcaaa tcatggaaac gatttaccat acgtgtcgcc aaacagcagc
    13621 cgagtacggc gacgaaaaaa atctgcttct tggagcaaat gttgctggat ttgaaaaagt
    13681 tgccaaagca atggcagcag aaggtttagt ttgaaaaatg aaactttctt ggaaatattg
    13741 tcagcatttt gtttatttgc ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag
    13801 agtaaaccta taaggaggag aacaaatggc acacattcat tttgactatt caaaagtagc
    13861 accgtttgtc aacgaacaag agttagggta catgcaaagc caagtaacag cagcccacaa
    13921 cgaattacgg gaaggtactg gcgcaggaag cgacttccgc ggctggatcg atttaccaac
    13981 gaactatgac aaagaagaat ttgcacgtat caaagaagca gctaaaaaaa tccaatctga
    14041 ttctgaagta ttagttgtta tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat
    14101 cgacttcttg aatcacacat tcttcaattt attagatagc aacacaagaa aagcaccaca
    14161 aatcttcttt gccggaaatt caatcagctc aacatatatt gctgacttga tcgaagtaat
    14221 cggtgatcgc gacttttcag taaacgttat ttcaaaatct ggaacaacta ctgaaccagc
    14281 gattgcgttc cgtgtattca aagagcttct tgttaagaaa tatggacaag aagaagcaaa
    14341 caaacgtatc tacgctacaa cagataaagc aaaaggtgca gttaaagtag aagcagatgc
    14401 agaaggttgg gaaactttcg tgattcctga tgacatcggc ggacgtttct ctgtattaac
    14461 tgctgttggt ttgttaccaa tcgctgttag tggtgcagat atcgatgcat tgatgcaagg
    14521 tgccgctgat gcaagcaaag catactcaag cgacaaacta gaagaaaacg aagcttacca
    14581 atacgcagca atgcgcaaca ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa
    14641 ctatgaacca ggaatgcaat acttctcaga atggtggaaa caattgtatg gtgaatctga
    14701 aggaaaagac caaaaaggca tctatccttc aagcgcaaac ttctcaactg atttgcactc
    14761 tttaggacaa ttcatccaag aaggacgtcg caatattttt gaaacagttg tgaaggttga
    14821 aaaacctcgt aaatcaatca caattcctga acaagcagaa gatttagacg gtctaggcta
    14881 cttgcaagga aaagaagtgg acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc
    14941 tcatacagac ggtgacgtgc ctaacttgct agtcaaaatt ccagaaatgg atgcttatac
    15001 attgggttat acaatgtact tctttgaaat tgctgtaggt atttcaggtt acttgaatgg
    15061 aatcaaccca ttcgaccaac caggtgtaga agcttacaag aaaaacatgt tcgcacttct
    15121 aggcaaacca ggctttgaag atcttgctaa agaattgaac gaacgcctgt aagataaggg
    15181 tttttcgcta gatttaaata atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg
    15241 tacccattta ggtactcaaa catactgcat cgtttattca gaataagggg ctgtgacaag
    15301 acgtttgtca cagtcccttg tttgtataga aatcgattga ttgccttgct ctttagtttg
    15361 ttttaggact ttaagaacag tgaggctgac ttcgaaggtt aattctgttt ttttgttttc
    15421 tagaatcagt aagcaaaaaa gcactcactt cgagtgcttt tttgcgatat attcttagct
    15481 taagataggc ctaatttttc ttttacagat gagggtacct tatcgctggt aattactttt
    15541 ttctctatga tccgcttttc gcttgctttc aactgcaaga tcgttccttc ggggtaagca
    15601 gaaccattgg cagagtcacc agaagtaatg acatcgaaat ctaactgtct tttttcaccg
    15661 ttttcagcat aagcatctac gttgtattta aaacctcttc caattacttc cccattatct
    15721 gctttgatat cagtttctgc aggcattgga gctttgacta ccgcataata atctttacct
    15781 acataagtag actgatagta gtcccaacct ttccatccta taaaagcaat caatatgata
    15841 acgatcaacc ctaaaatttt tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DACDTH010000066        14930 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDTH010000066 DACDTH010000000
VERSION     DACDTH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.066"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            8..487
                     /locus_tag="IWK54_002243"
     CDS             8..487
                     /locus_tag="IWK54_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9594343.1"
                     /translation="MVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITNDLKLSTSMIHL
                     IDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNKKTKLVSLTKY
                     GRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEYQVSLTRKED"
     gene            580..1742
                     /locus_tag="IWK54_002244"
     CDS             join(580..856,856..1742)
                     /locus_tag="IWK54_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9594344.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2022..3002
                     /locus_tag="IWK54_002245"
     CDS             2022..3002
                     /locus_tag="IWK54_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9594345.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3124..4542
                     /locus_tag="IWK54_002246"
     CDS             3124..4542
                     /locus_tag="IWK54_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9594346.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4613..6082
                     /locus_tag="IWK54_002247"
     CDS             4613..6082
                     /locus_tag="IWK54_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9594347.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6092..6559
                     /locus_tag="IWK54_002248"
     CDS             6092..6559
                     /locus_tag="IWK54_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9594348.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6595..7467
                     /locus_tag="IWK54_002249"
     CDS             6595..7467
                     /locus_tag="IWK54_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9594349.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7548..10082
                     /locus_tag="IWK54_002250"
     CDS             7548..10082
                     /locus_tag="IWK54_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9594350.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMCQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10238..11011
                     /locus_tag="IWK54_002251"
     CDS             10238..11011
                     /locus_tag="IWK54_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9594351.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11004..11795
                     /locus_tag="IWK54_002252"
     CDS             11004..11795
                     /locus_tag="IWK54_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9594352.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            11805..12296
                     /locus_tag="IWK54_002253"
     CDS             11805..12296
                     /locus_tag="IWK54_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9594353.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12314..12724
                     /locus_tag="IWK54_002254"
     CDS             12314..12724
                     /locus_tag="IWK54_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9594354.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            12728..14128
                     /locus_tag="IWK54_002255"
     CDS             12728..14128
                     /locus_tag="IWK54_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9594355.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14151..14525
                     /locus_tag="IWK54_002256"
     CDS             14151..14525
                     /locus_tag="IWK54_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9594356.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5187 a         2101 c         3000 g         4642 t
ORIGIN      
        1 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
       61 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      121 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      181 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      241 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      301 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      361 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      421 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      481 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      541 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      601 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      661 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
      721 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
      781 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
      841 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
      901 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
      961 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1021 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1081 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1141 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1201 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1261 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1321 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1381 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1441 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1501 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1561 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1621 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1681 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     1741 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     1801 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     1861 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     1921 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     1981 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2041 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2101 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2161 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2221 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2281 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2341 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2401 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2461 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2521 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2581 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2641 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     2701 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     2761 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     2821 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     2881 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     2941 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3001 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3061 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3121 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3181 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3241 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3301 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3361 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3421 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3481 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3541 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3601 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3661 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     3721 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     3781 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     3841 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     3901 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     3961 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4021 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4081 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4141 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4201 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4261 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4321 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4381 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4441 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4501 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4561 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4621 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4681 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     4741 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     4801 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     4861 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     4921 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     4981 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5041 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5101 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5161 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5221 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5281 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5341 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5401 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5461 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5521 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5581 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5641 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     5701 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     5761 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     5821 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     5881 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     5941 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6001 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6061 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6121 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6181 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6241 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6301 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6361 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6421 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6481 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6541 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6601 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6661 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     6721 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     6781 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     6841 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     6901 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     6961 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7021 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7081 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7141 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7201 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7261 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7321 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7381 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7441 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7501 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7561 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7621 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7681 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     7741 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     7801 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     7861 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     7921 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     7981 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8041 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8101 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8161 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8221 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8281 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8341 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8401 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8461 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8521 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8581 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8641 ttgaagaaag catgtgtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     8701 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     8761 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     8821 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     8881 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     8941 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9001 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9061 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9121 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9181 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9241 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9301 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9361 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9421 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9481 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9541 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9601 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9661 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
     9721 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
     9781 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
     9841 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
     9901 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
     9961 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10021 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10081 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10141 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10201 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10261 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10321 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10381 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10441 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10501 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10561 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10621 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10681 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    10741 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    10801 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    10861 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    10921 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    10981 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11041 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11101 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11161 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11221 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11281 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11341 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11401 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11461 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11521 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11581 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11641 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    11701 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    11761 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    11821 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    11881 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    11941 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12001 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12061 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12121 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12181 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12241 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12301 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12361 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12421 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12481 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12541 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12601 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12661 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    12721 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    12781 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    12841 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    12901 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    12961 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13021 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13081 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13141 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13201 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13261 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13321 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13381 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13441 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13501 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13561 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13621 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13681 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    13741 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    13801 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    13861 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    13921 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    13981 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14041 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14101 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14161 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14221 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14281 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14341 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14401 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14461 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14521 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14581 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14641 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    14701 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    14761 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    14821 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    14881 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DACDTH010000067        14503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDTH010000067 DACDTH010000000
VERSION     DACDTH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.067"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(308..670)
                     /locus_tag="IWK54_002257"
     CDS             complement(308..670)
                     /locus_tag="IWK54_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594357.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(744..1124)
                     /locus_tag="IWK54_002258"
     CDS             complement(744..1124)
                     /locus_tag="IWK54_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9594358.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1206..2381
                     /locus_tag="IWK54_002259"
     CDS             1206..2381
                     /locus_tag="IWK54_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9594359.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2458..3042)
                     /locus_tag="IWK54_002260"
     CDS             complement(2458..3042)
                     /locus_tag="IWK54_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9594360.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3243..4241
                     /locus_tag="IWK54_002261"
     CDS             3243..4241
                     /locus_tag="IWK54_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9594361.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4353..4862
                     /locus_tag="IWK54_002262"
     CDS             4353..4862
                     /locus_tag="IWK54_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9594362.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4892..5551)
                     /locus_tag="IWK54_002263"
     CDS             complement(4892..5551)
                     /locus_tag="IWK54_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9594363.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5553..6317)
                     /locus_tag="IWK54_002264"
     CDS             complement(5553..6317)
                     /locus_tag="IWK54_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9594364.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6325..7002)
                     /locus_tag="IWK54_002265"
     CDS             complement(6325..7002)
                     /locus_tag="IWK54_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9594365.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7039..8424)
                     /locus_tag="IWK54_002266"
     CDS             complement(7039..8424)
                     /locus_tag="IWK54_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9594366.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8634..8718)
                     /gene="ffs"
                     /locus_tag="IWK54_002267"
     ncRNA           complement(8634..8718)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWK54_002267"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8741..9256)
                     /gene="tadA"
                     /locus_tag="IWK54_002268"
     CDS             complement(8741..9256)
                     /gene="tadA"
                     /locus_tag="IWK54_002268"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9594367.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9363..10220
                     /locus_tag="IWK54_002269"
     CDS             9363..10220
                     /locus_tag="IWK54_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9594368.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10301..10636)
                     /locus_tag="IWK54_002270"
     CDS             complement(10301..10636)
                     /locus_tag="IWK54_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594369.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10703..12073)
                     /locus_tag="IWK54_002271"
     CDS             complement(10703..12073)
                     /locus_tag="IWK54_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9594370.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12151..12687)
                     /locus_tag="IWK54_002272"
     CDS             complement(12151..12687)
                     /locus_tag="IWK54_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9594371.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12996..14477
                     /locus_tag="IWK54_002273"
     CDS             12996..14477
                     /locus_tag="IWK54_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9594372.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4470 a         2886 c         2355 g         4792 t
ORIGIN      
        1 ttagtaacat gtcaataact attttttaaa ttttttagaa atgtgatata acatccactt
       61 cagaatcaac gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca
      121 ttactttgtg aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac
      181 atattttgaa aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt
      241 ttggaccaaa caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat
      301 ctgtatttta gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt
      361 gacctggttc ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag
      421 tttcaaagac aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca
      481 aactaatctt gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta
      541 aaccagtctt tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat
      601 ggaccaaaaa ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt
      661 tgcttgccaa aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca
      721 cattttcaga aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat
      781 gcttcttcta tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt
      841 ttattcgtga aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt
      901 cttaaagata aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact
      961 ggctgtccga cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa
     1021 acatgaataa gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc
     1081 cctgtcactt ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc
     1141 gcatttatca ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc
     1201 ttaagatgat cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg
     1261 acgcaattgc tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg
     1321 acttcttgtc tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct
     1381 ttggttatgc cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac
     1441 accatgctgt ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt
     1501 tagctgcagc agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag
     1561 tttatcctcc attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt
     1621 tgctagaaaa aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag
     1681 aaaacaatgt aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga
     1741 gcaaagaaga attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta
     1801 gtgacgagat ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga
     1861 ttgatcctaa tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc
     1921 tagctgcaat caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg
     1981 aaaaacattt agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc
     2041 aagcggctta tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa
     2101 atgttcaaac agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc
     2161 ctgaaggaac ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat
     2221 tggagaacac tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc
     2281 cttccggtca tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag
     2341 gacttaatcg aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa
     2401 aggacgagac cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta
     2461 ttctgaaatt tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg
     2521 ttgtacattt gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt
     2581 atgtcggaaa tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg
     2641 gaatccagca ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat
     2701 aaagaattgg cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag
     2761 gttgaacagt tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc
     2821 cattcctgtt ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa
     2881 aatcacgcca tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg
     2941 tgctagttca gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt
     3001 tggtcctttt tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat
     3061 ttgcatactt cataataata ataacataat acctaacctc tcgtaaaaat actagatttg
     3121 ttttctttta tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc
     3181 aaacattttt gagaagcatt ctcaattaaa ttctgctata gtgtgaaaag ggaggaattt
     3241 ttatggaaat ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt
     3301 tttacgcagg aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg
     3361 gtcaattagc aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa
     3421 taaaagcaag tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat
     3481 tccatttaaa agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta
     3541 caaataaagg catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc
     3601 agccaagaaa attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt
     3661 tcgtgaatga tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt
     3721 ctgctatcga ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc
     3781 gtcgcccgga atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca
     3841 acttgcttac tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata
     3901 tccggttaaa aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg
     3961 tcaattatgg attctcttct aatttagaac atctttcttc ttggggactt gatagtacca
     4021 gaaatgccat tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg
     4081 gtgacatctg tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac
     4141 ctactgcagt gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc
     4201 acagcacaag cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca
     4261 tttcaagtta aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat
     4321 agagaaagaa aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag
     4381 aaaaggcaag agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg
     4441 tcatgttttt acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg
     4501 tcaacagcgt gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag
     4561 atatattagc agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg
     4621 aaggccttta cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga
     4681 tcggctttac gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa
     4741 acagccacga tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg
     4801 ctgctgctgc cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat
     4861 aattcaacaa aaaaagaaag aaaacgtcta tttagacgag ttctttcttt tttgtttgtt
     4921 tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa
     4981 ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa
     5041 cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc
     5101 gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa
     5161 acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac
     5221 tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat
     5281 aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga
     5341 agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta
     5401 aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt
     5461 tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat
     5521 attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt
     5581 ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat
     5641 aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta
     5701 tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata
     5761 atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa
     5821 gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg
     5881 ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca
     5941 taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta
     6001 aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc
     6061 ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct
     6121 ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta
     6181 gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat
     6241 cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa
     6301 tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt
     6361 tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga
     6421 ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt
     6481 ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc
     6541 ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta
     6601 aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg
     6661 gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg
     6721 ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc
     6781 ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac
     6841 tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt
     6901 cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt
     6961 tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt
     7021 ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac
     7081 acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa
     7141 aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat
     7201 tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac
     7261 catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa
     7321 gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac
     7381 atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc
     7441 ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg
     7501 ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact
     7561 cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag
     7621 ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc
     7681 tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac
     7741 atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct
     7801 gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat
     7861 ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg
     7921 taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg
     7981 attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt
     8041 cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa
     8101 taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt
     8161 ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat
     8221 cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa
     8281 atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg
     8341 ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt
     8401 atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat
     8461 aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca
     8521 aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt
     8581 ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat
     8641 cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag
     8701 cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc
     8761 agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca
     8821 tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt
     8881 cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat
     8941 aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg
     9001 taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga
     9061 ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc
     9121 ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt
     9181 ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa
     9241 gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata
     9301 tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa gaggtgaaca
     9361 agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa aactctccag
     9421 catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg atcaaacaag
     9481 attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta atcaacgatc
     9541 aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt caagaaaaaa
     9601 gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa aaagaatggc
     9661 aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat acagacacag
     9721 atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa atcaacggta
     9781 tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt ggttctttcc
     9841 attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt tttttaacag
     9901 agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg gctttacatt
     9961 actatacaaa agatactgta aaagatagta agttgatcaa aagttacata gaactgatca
    10021 aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc aatgtcagct
    10081 ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc gaaaaatttc
    10141 aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc tatcttttat
    10201 tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt ttgtcacaac
    10261 cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt ttttcaacaa
    10321 aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca ccataaacag
    10381 agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa tccaagtcac
    10441 tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat ttgtcataga
    10501 cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt acttttcccg
    10561 atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg attgttactt
    10621 ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata gcatagacaa
    10681 aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa aagttcttta
    10741 ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact agcaaaaccc
    10801 atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc cattcctttt
    10861 gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct aatgcatagc
    10921 taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt acgactttta
    10981 aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg aacactaaaa
    11041 gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt ttttcctgct
    11101 cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc atctgatttt
    11161 gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta tgaggccata
    11221 tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct gaactaacgg
    11281 attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc cagctaatga
    11341 tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt cctttatttt
    11401 ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag tatagcggag
    11461 attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct tctcctgaac
    11521 aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca agttctgcgg
    11581 gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt tctgataaaa
    11641 tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc ttcatttgag
    11701 cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact ttttcaccgg
    11761 gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta tattcatacc
    11821 cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca ttgctgtagg
    11881 gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct gccggtttta
    11941 gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa aaaaaatcgg
    12001 atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt aacgttgtcg
    12061 cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac gaaaaataaa
    12121 ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta agatattttc
    12181 caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt caagaaaatc
    12241 ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca attcaagcga
    12301 cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc gcttaaatag
    12361 cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat ggatagaaaa
    12421 aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt cagctaagta
    12481 gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc tgattgattt
    12541 gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact ctcttgcagt
    12601 aattgacttc attccatatt cttccacgta taccatactt ttgaacagca aatattcttt
    12661 actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc tacccataaa
    12721 taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata tttagttata
    12781 tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa ataccctagg
    12841 aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata taaaaaagaa
    12901 ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag tttttgtcta
    12961 actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca agaaaaaaag
    13021 agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa gaattatcca
    13081 atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata aatgtatcac
    13141 tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga gagatccaaa
    13201 ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa ttatgctacc
    13261 taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca caaactgctg
    13321 taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc gaaaaactga
    13381 caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct gtaaaaaatt
    13441 gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt tttctttcag
    13501 atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca ctaaaaatga
    13561 acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc tacttgctgt
    13621 taactttatt catattacga aacaaacaac aggcatattt tccaaaaact tcttctgaaa
    13681 tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac tttacttctt
    13741 tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt aattattttt
    13801 tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt ggttttttat
    13861 tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact tacttaactc
    13921 aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat tttcttgtgt
    13981 tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct acactttgct
    14041 ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa ttaagagaaa
    14101 aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa taccttagtc
    14161 aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc catttgcaga
    14221 taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag ttatttcgca
    14281 aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt acagatcagc
    14341 atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac aatacagtgt
    14401 tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt tatgagaaca
    14461 aaacaaagag gatgtgacaa aattcttgtc acatcctctt att
//
LOCUS       DACDTH010000068        14463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDTH010000068 DACDTH010000000
VERSION     DACDTH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.068"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(27..602)
                     /locus_tag="IWK54_002274"
     CDS             complement(27..602)
                     /locus_tag="IWK54_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9594373.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(607..1581)
                     /locus_tag="IWK54_002275"
     CDS             complement(607..1581)
                     /locus_tag="IWK54_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9594374.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1605..1649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1864..2832
                     /locus_tag="IWK54_002276"
     CDS             1864..2832
                     /locus_tag="IWK54_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9594375.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2945..3223
                     /locus_tag="IWK54_002277"
     CDS             2945..3223
                     /locus_tag="IWK54_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9594376.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3341..3577
                     /gene="secG"
                     /locus_tag="IWK54_002278"
     CDS             3341..3577
                     /gene="secG"
                     /locus_tag="IWK54_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9594377.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3689..4435
                     /locus_tag="IWK54_002279"
     CDS             3689..4435
                     /locus_tag="IWK54_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9594378.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4438..6795
                     /gene="rnr"
                     /locus_tag="IWK54_002280"
     CDS             4438..6795
                     /gene="rnr"
                     /locus_tag="IWK54_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP9594379.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6817..7281
                     /gene="smpB"
                     /locus_tag="IWK54_002281"
     CDS             6817..7281
                     /gene="smpB"
                     /locus_tag="IWK54_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP9594380.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7583..8302
                     /gene="atpB"
                     /locus_tag="IWK54_002282"
     CDS             7583..8302
                     /gene="atpB"
                     /locus_tag="IWK54_002282"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9594381.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8355..8570
                     /gene="atpE"
                     /locus_tag="IWK54_002283"
     CDS             8355..8570
                     /gene="atpE"
                     /locus_tag="IWK54_002283"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9594382.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            8661..9185
                     /gene="atpF"
                     /locus_tag="IWK54_002284"
     CDS             8661..9185
                     /gene="atpF"
                     /locus_tag="IWK54_002284"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9594383.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9172..9714
                     /locus_tag="IWK54_002285"
     CDS             9172..9714
                     /locus_tag="IWK54_002285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9594384.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            9737..11293
                     /locus_tag="IWK54_002286"
     CDS             9737..11293
                     /locus_tag="IWK54_002286"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9594385.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            11307..12209
                     /locus_tag="IWK54_002287"
     CDS             11307..12209
                     /locus_tag="IWK54_002287"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9594386.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12232..13638
                     /gene="atpD"
                     /locus_tag="IWK54_002288"
     CDS             12232..13638
                     /gene="atpD"
                     /locus_tag="IWK54_002288"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9594387.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13652..14074
                     /locus_tag="IWK54_002289"
     CDS             13652..14074
                     /locus_tag="IWK54_002289"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9594388.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4781 a         2590 c         3123 g         3969 t
ORIGIN      
        1 tattttgcta atccagctct gtttattcag cctatttttc tttttgaata tatcaagcgt
       61 tgtggcaaca tagtgatgac taatgaaaca acgacgatag acaacaaacg atcaaggtag
      121 tctgtacctg cctgtaccaa gatcacactt gttgtctgac ttaaacctaa cccatgtaat
      181 agctgaacga tcatactgga tcctgaggag gtgatcccgc caaataactg aacggtaatg
      241 atcgaagaaa cgatcgttcc aggtaccgac aaaatcaaag cgagtaataa cagtttcaag
      301 ctgtttttat ctttgccgag atatcttggc aaaatcaatc cagcaagcaa gcctgtgaga
      361 atttggattg gcgaataata cagagaaaaa atatctgtag tcactccact taaaataccg
      421 ctggacaatc ctgtcagcat tccaggtaca ggcccaagca atgcaccagc aaaaatcgtt
      481 ccgatagaat cgagatagat tggcaaacga agccataaag caatcgttcc tccaatataa
      541 ttgatcccaa tacacaaagc catcactgta agtacgcgag ttgccatttt gtttttattc
      601 attttgttag cccatcttta acctttccaa atctttcata atcgtttctt cttgtgcatt
      661 taaaagaacg gttaaaaact ttcggaaaaa aagacgagta tctacttttg tcataatttt
      721 actgttagca ggtttctgcc aaaattcata acgatcaacc aatgtttgtc cacgacttat
      781 tccttgagtt tccacagcag tgaagctttt gaaaccttca cagatggccc cctcgataaa
      841 ataagcaaca gctaacggat cattgatgac acatcctaaa attctttctt gtttccagtg
      901 gaagtcaaaa taaaaacgag tgatcgcttt tagatactct ccctcttcag gattcatttg
      961 acagcagtat tcgaggatag tcggtgtcaa gacgatttct cttgtcacat ctaacccaac
     1021 catttctatc gtttgtttga gattctcgaa tacataagaa gctgcttcag gatcacacca
     1081 gtaattgtat tcagcaactg gcgaacagtt accatggctt ttgtaagttc cacccatcga
     1141 gacaaaacga tccatatgtt tcccaatatt tgggttgact ttcaaagcaa aagcgatatt
     1201 ggtcaaagga cccaaagcaa tgactgatgt atttgtcttt tctttgaaat aatcagccaa
     1261 aaaatcaacg gcatggagag gttctgcctg ttttctcaag atcatcggga aatttgtttc
     1321 ccctaaacca tccataccat gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt
     1381 ttccattcct tgataaacag gaatgtctaa acgatcacag cgttccaggc atttcagcac
     1441 attctgcgta cctatatctg cagggacatt cccacaaact acggtaatag caactacttc
     1501 gatatcaggg gatttgacag ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca
     1561 atcaataatt acttttcgca ttatttccaa tctcctttct ttagagatac ttagtttttt
     1621 attctgggaa gtttccgaac cagtccaaga cgagcgtctt gattataatg cacgaaatta
     1681 gtatactggc ttttattgcg aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa
     1741 ctttaagtaa atgatacaat gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat
     1801 cctatggatc ggtacattgg ataatagtta gattatagtt tggcagaaag cggcgggtgt
     1861 acaatggtta ttttttattt cattcttatt ctgataatta tagggatcag ttaccatttt
     1921 ctttcttatt tttatgcaaa gaaattagta agagcgggag tacagagagg atatgactgg
     1981 tatgaagaca ctggtcatcg tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa
     2041 cgacaaaaat tagaagaaga gtgtgaaagc ttttgggagc agggaacacc caaaacaatc
     2101 aaaagttatg acgggttgaa actagctggt cagatgttca tccatccaaa taagcagaat
     2161 aaatgggtca tttgcgtaca tgattaccga agcacaggga aaagagacat gtctcatata
     2221 ggtaaaagat atgcagagaa aggattcaac gttcttgtac ctgatttacg tgcgcacgga
     2281 gaaagtgaag gagaaatcat cggaatggga tggctagatc ggctagattt aattgcctgg
     2341 atccaactca tcttagatga acagccgaat gcgtccatta ttttacacgg cggatcaatg
     2401 ggagcttcta cgatcatgat ggcaagtggt gaaaaacttc catcagcggt caaaggattt
     2461 attttagaca gcggatatgt ttctgtttat gcagaattcc gatatatgtt aagtaaaatc
     2521 actgtctttc ctaaaaaaat ggtcatgaga tatgcgaatc attatgccca gaaatacgcc
     2581 ggttactcct taaagcaagc atcagctacg agacagcttg gcagcaacca tctgccttta
     2641 ttggtcattc atggtgaaag agatcatttt gtccctacag aagcagcgta cacgattcaa
     2701 aatgctacgg caggggataa agcactgctg cttgttccag aagcagaaca cttggaagca
     2761 tcgatgaaag atccgaaaac ctattggaca gtgatttttt catttattga acaaagaata
     2821 acgatgaatt aattttctct tcttttttgt aagtaattcg aggataaata attgtttata
     2881 gatcaggaga ctgctaaaat aaggtaggaa taaaaaaata tcagttattt gaggagtgcg
     2941 gcatatggaa atcaaagaag aaaaaaatcg tcttgcttta ttcaatgatg aacaacaaga
     3001 gatcggagaa atgacatggt cagatgcggg cccagacatc atgatcattg accatacatt
     3061 tgtagatcca gcatatcgtg gtcaaaagtt agctgaaaag ctagtgtata ctggagtaga
     3121 attagcaaga cgagacggga aaaagatcat tcctttatgc ccttatgcaa aaaaagagtt
     3181 tgaaaagaaa ccagaatatc aagacgtctt acgtcagtca taagaataac agctgaaagt
     3241 tttgaacttt cagctgttat tccatttata tagagttgtc acggactttt atctatgata
     3301 aagttaaaga gacgatatct tttgggaagg aggctgcgat atgtataatt taattttggg
     3361 aatcgtgatc gttttatcga ttgttatggt cattgcgatt atgatgcagc caagcaagca
     3421 aaatagtgca gcgagtgcat ttaccggcgg tgcggatcaa ctattcggca aacaaaaagc
     3481 tcgcggcttt gaagctgtca tgcagcgatc aactgcagtt atgggagcta tctggatgat
     3541 cttattattt gtcctagcat atttatcttc gaagtagttg aataagcctc tcgcaaaacg
     3601 gcgggcggct ttttttattg caaagagaac gcttaagaag aagttatgaa agaaactatg
     3661 gtaaaatgag gcgaaagagg tgagacgagt gagacaatta ccaaaacccc tttatgcaaa
     3721 acatggaaag cgagcggttt tgctgcttca cgcttactca ggaagtccaa acgatgttcg
     3781 aatgctcgca cgatttttgg aaaaagcaga ttatacagtt tatgcgccgc tttttaaagg
     3841 acacggaaca atggagccat atgatatttt gcaagaaaaa gcagaaagct ggtgggcaga
     3901 cactaaaaaa gccatccatt ttttacagtc tgagcagttt tctgatattg ccgtattagg
     3961 attatcaatg ggcggcatct ttgctgtacg agcactagaa gaagaatcag tgattggcgg
     4021 cggttttttc tgttcgcctt tgtctcctgt aaaaacaaac gtgcctgaaa actttgaaaa
     4081 atacgtacgt caagtgctga agacagcagg taaatcagag aaagaaatca atgaaaaagc
     4141 agttgcttac cgctcgttag ctgaacaaca attaatggat atacaagatc aggcagcaat
     4201 cgtagaaagt aggttgtcag atatccaaca gccgatattt ttagctcaag ctgggaaaga
     4261 tgaaatgatc gaccctaatg gcgtatttga aactgcacgc aagttgagcc gtcagcgagt
     4321 gacacttcga tggtatcctg aaagtggtca tgtgattaca gttggtacag caagacgcga
     4381 attagaaaaa gacgtcttgg aatttttaga aaaattacct tggaatgagg aataaatatg
     4441 acaaaagaaa cgataaaaga gaaaattctc tttttcatgg aaagcagtcg caaacgcagt
     4501 ttttctatgg aagaaatcgc tgaaggttta gggttccaaa aaagcgatga ttttaaatta
     4561 ctagtacaaa cgattgctca aatggaaaga gaacagagta tagttttcaa taaaaaagga
     4621 aaagtgaaac ttcctttaaa accaatcttg atcgaaggaa catttcgagc aaatgaacga
     4681 ggatttggat ttgtaacgat tgatcccgaa gaagatgaca tctatatccc aaaagaagcg
     4741 actggttttg caatggatgg agacaccgta gcaatcgata tcatgaagcg agcaaataca
     4801 gatatggatc gcggagcaga ggggaaagta gtcgagattc gagaccgggc gactactcag
     4861 ttagcagggg aattcgttgc ttattcagaa gaagaaatac aagaaacgga tctttacggc
     4921 gttgttatcc cgaaagatag aaagctcaac caatttaaag tatatgcagc agcagaggga
     4981 gttcgtccag ttgacggaag tattgtgctg atcgaactga cccattaccc tgaaaaaggg
     5041 tatgcgacta gtttagaagg gataatcaag caagtgattg gtcataagaa tgaccctggc
     5101 atggatatcc tctcaattgt cgtggctaat ggtatcccta cgaaatttcc tgatgatgta
     5161 ttgtctgaag cagatgaagt tccagatcaa atcagcgaaa aggatataac tggacgtaga
     5221 gatttaagaa atcagctcat tgttacgatt gatggagaag atgcaaaaga tctagatgat
     5281 gcagttacgg ttaaaaaatt agataacggt aattattttc ttggagtaca cattgcagat
     5341 gtttcttact atgtgacaca aggcagtcaa ttggacagag aagcatatga aagagggaca
     5401 agtgtatatt tgactgatcg agtgattccg atgatcccgc aacgtttatc gaatggtatc
     5461 tgttcactga atccccatgt tccaaggctg accatgagtt gcgaaatgga gattgatcca
     5521 aatggacaag tagtctctca tgatattttc ccaagtgtga tccaaacaac agaacggatg
     5581 acctacacag ctgtcaatca aatcttagaa gatcaagacg aagaaacgat gcaacgctat
     5641 gctcagctcg tacctatgtt ccaagaaatg ggtgaattgc atcgaatcct tgaaacgatg
     5701 cgaatcaaac gtggcgcgat ttcttttgag gatcgcgaag caaaaatcct tgttgatgct
     5761 gaaggacacc caaatgatat ccagctaaga agcagaggaa ttggtgaacg cttgatcgaa
     5821 tctttcatgc ttgcggccaa tgaaacggta gctgaacatt ataataaatt aaatctgccg
     5881 tttatttacc gaatccatga acagccaaaa gaagaaaaaa tgcagcgctt ctttgatttt
     5941 gcttcagctt tgggcattct ggtccgagga acgaaaggaa cgatcacacc aaaagattta
     6001 cagcatgtga tcgaaggagt agaagacaaa ccagaagctg cagttatcaa tactatgttg
     6061 ctaagaagca tgcaacaagc acgctattca gaagacaatt ttgggcatta tggcttagcg
     6121 gcagattact acacacactt tacatctcct attcgacgct atcccgatct aatcgtccat
     6181 cgtctgatcc gttcttacag tgaagataaa agtgaaagca atcaaagttc gtgggctacg
     6241 gaacttcctg aaattgcaga tcatagttct aagatggaac gtcgtgcagt agaaacagaa
     6301 cgtgaagtag attccatgaa gaaagcagaa ttcatgatgg acaaagtggg ggaagaattc
     6361 gatgggatca tcagctctgt tgtgaaattc ggcctgttta tcgaactgcc gaatacagta
     6421 gagggattga tccatatcaa tgaattgaaa caggactact tccattttgt tgaaaatcat
     6481 ttagctcttg taggtgaaag aactggtctg acttttaaaa tcggacagaa agtgcgagta
     6541 aaagtagtca aagctgatcc agatgaacga gcagtagatt ttgaacttgt ttccgcagaa
     6601 gaggttacag cgttggaacg gccaaaaaca cgaaatggaa aatcaggaaa acgcgaagga
     6661 aaacaagcag gaaaacgccc agaaagaaat ggcaagaaga acaaacgata tagcgatagt
     6721 aagcaaacgt tcggtaagaa aaaagggaag aagccatttt acaaaggtgt taaaaagaag
     6781 aaaaagaaaa aataaaatga gttggaggaa acagctatgc caaaaggcga gggaaaatta
     6841 attgcacaaa acaagaaagc tcgccatgat tattcgatca tcgacacgat ggaagcaggg
     6901 atggtcttgc aaggaaccga gatcaagtcg atacgaaaca gccggatcaa tctaaaagat
     6961 ggatttattc gcgtccgcaa cggagaagct ttcttgcata atgttcatat cagtccttat
     7021 gaacaaggaa atatttttaa tcatgatccg ttgcgcacga gaaagttatt attacacaaa
     7081 aaacaaatca tccggcttga aaatgaattg aaaaatactg gaatcactgt tgttccttta
     7141 aaagtctata ttcgtaacgg ctatgccaag gtattgattg gtctggcgaa agggaaaaaa
     7201 tcttatgata aacgggaaga tttgaaacga aaagatatcg atcgacaaat tgatcgaaca
     7261 ttaaaaaatt tctctagata atttcattat cctttttcgc atattcggtg cttttgcttt
     7321 tacttaatga taaaatttgt tagcatttaa tctgataaaa catgattatc ataggttttt
     7381 tcttagatca ggagcaccgt gtataaagaa aaattcgctt cgtttttgac tgaaataaaa
     7441 aagtgtctaa ttttcatgaa aatgttttga attttctgtt tcatggtggt atgataccgt
     7501 gtggttagaa accgattgga aaaaaccaga tacgcacatg aattgatttt atgtgaattt
     7561 cggggaaaag aggtgaaatg gattggatga acgctcgcta acgttccaca ttggaccaat
     7621 ttggtttgat ggtacagttt gtataatggt gttactgacc tgtttgattg ttttcttctt
     7681 agcttacttt tttacaagaa atcttaagtt gaaaccaaca ggcaaacaaa atgcgataga
     7741 atgggtcatt gattttagtc gaggaatcgt aacagataat ctgcctagaa aagaattgag
     7801 caatttccat ttgttggcat ttacattgtt tttatttgtt tttgttgcaa acaacatcgg
     7861 tttagtaaca aaaatcgttt tacctaacga aacaacactt tggaaaagcc cgacagcaga
     7921 cccgtttgtt actttaactt tggcatttat aatgatctta cttactcact tatttggggt
     7981 gaaaaagtta ggcttcaaag gatatttcgt aaattcgttc ttgaaaccat atagttttat
     8041 gtttccgatg aaactgattg aagaatttac aaacttatta acacttgctt tacgtcttta
     8101 tgggaatatt tatgcggggg aagttttgct aaccttgatt gcaaaaatgg taataagctt
     8161 aggctggttc tcattgccgt tagcaattcc attagagatg gtctggatcg cattctcctt
     8221 gtttatcgga agcatccaag catttgtctt cgttacttta tctatggttt atatgagcca
     8281 taagattgaa gtggaagaat aatattcgtt ttaaaaacaa actactattt atctaattta
     8341 ggaggaaaac aattatgaat tatatcgcag cagcaatcgc aatcatggga gcagcaatcg
     8401 gagccggtta tggtaacggt caagttatct ctaaaacaat cgaatcaatg gctcgccaac
     8461 ctgaaatgtc tggtcaattc cgtacaacaa tgtttatcgg ggtagctcta gtcgaagctg
     8521 ttcctatctt aggggtagtt gtcgccttga ttctagtttt tggtgtttaa ttgaatcagt
     8581 attaaggcag aaacaatttg gtttgttact gcttttttac atgaaaacta gaagtacttc
     8641 aaagaaagga aggctcgcct atgctgaatc aattggcaat tgcagaagtt ggcaatgcga
     8701 tgttaggaaa tgtcatcgtt gtcagcggct catttttgat actactggct ctcttgaagc
     8761 attttgcttg gggaccaatc agcgatattt tgaaaaaacg tgaagacaag atcgccaatg
     8821 atttggattc tgcagaacaa tctcgcatca actcagcgaa aatggaacaa gaacgcgaac
     8881 aacaattgtt agcctctcgt tctgatgcag ctgatatcat caaaaatgcg aaagaaagtg
     8941 gagaattaag ccgccaaaat attttgaagg atgctcaaga agaagcagct cgtctaaaaa
     9001 gtaaagccca agctgatatc actgtagaac gtgattcagc gctgaactct gtaaaagacg
     9061 atgttgcaga actatctctt caaatcgcgg aaaaaatctt gaacaaagaa ttatctccag
     9121 aaatgcacga atcattaatc aatcaatata tcgaaggtct aggttcttca aatgaaacta
     9181 gataaatata cagtaggaag acgttacggg aaagctttat ttgaactggc gatcgattca
     9241 aataaagcag aagaaattta tcaagatctc gtaagcttac gtcaaatcta tgaacaagta
     9301 cctggattag gtaatatgtt aagtgatgtt cgtctagaac ctcatgaaaa acgagaaatc
     9361 atggacaagc ttgttagtgg ctatgatgga atcgttaaaa actttttaga agttgtttat
     9421 gcgtataacc gaatggatga tcttttgttc atgatcgaag aatatgaacg tcgatatgat
     9481 gaacatcaag gtttgctgtt gggaagtgtc acaacagcag ttccactttc agacgaacaa
     9541 cgaatacaat tagaaaagaa tgttgcaaaa acaatgaact atcaatcagt cgaattaaaa
     9601 caaatcgttg attcttctat tattggtggc gcgattgttg aagctaacca tcgagtaatc
     9661 gacggaagca ttcgcacgca attagaaaat atgcgtaatc aattaaatag atagtacaaa
     9721 gaggtaggtg aatcgaatgg ccatcaaagc agaagaaatt agtgccttga ttaaagaaca
     9781 aattgaaaat tatcaaaacg tgctttcagt cgaagaaatc ggtaccgtaa cttatgtagg
     9841 tgacgggatc gctcgtgcac atggtttaga aaacgcgatg agtggtgagt tgcttgaatt
     9901 ttcaaatggc tcatacggaa tggcgcaaaa cttagaatca aatgatgtag gtatcattat
     9961 ccttggcgat ttcgagtcca ttcgtgaagg agataaagta aaacgaacag gaaagatcat
    10021 ggaagttcca gttggagagg ccttgattgg acgggtagtt aatccgctag gtcagccaat
    10081 tgacggacta ggtgaaatcg ttactgacaa agctcgtcca gtagaagcaa tggcaccagg
    10141 cgttatgcaa cgtaaatctg ttaacgaacc aatgcaaaca ggtctaaaag caatcgacgc
    10201 tcttgtacca atcggacgcg gccaacgtga attagttatc ggtgaccgta aaacagggaa
    10261 aacttccatt gcgatcgata cgatcatcaa ccaaaaaggt caagatatga tctgtatcta
    10321 tgtagcaatc ggacaaaaag attctacagt tcgtacacaa gttgaaacat tgaaaaaata
    10381 tggcgcaatg gattacacaa tcgttgtgaa tgccggtgcg tctcaaccag cgccgttgct
    10441 ttatatcgca ccatatgctg gtactgcaat gggtgaagag ttcatgtaca acgggaaaca
    10501 tgtcttgatc atttttgatg atctttcaaa acaagctgtt gcttatcgtg aactttcctt
    10561 gctgcttcgc cgtccaccag gtcgtgaagc ttatccaggg gatgttttct acttacactc
    10621 acgcttattg gaacgtgcag caaaattaag tgatgattta ggtggcggtt caatgactgc
    10681 cttaccattt gttgaaacac aagctggaga tatctctgct tatatcccaa caaacgttat
    10741 ttcgatcact gatggacaaa tcttcttaga aagcgacttg ttctacgcag gtactcgtcc
    10801 agcggttgat gccggactat ctgtatctcg tgtagggggt tctgcgcaaa tcaaagcaat
    10861 gaaaaaagtt gctgggactt tacgtctaga cttagcaagt taccgtgaat tagaagcatt
    10921 tacacaattc ggttctgact tggatgcagc aacacaagca aaactaaatc gtggacgccg
    10981 tacagtagaa atcttgaaac aaaaattgca tgcaccgcta gctgttgaaa aacaagtagt
    11041 gattttgtat gctttgacac atggattctt agatagtatc cctgtagaca gtattttaga
    11101 cttcgaacat gaattattcg aatatctgga taccaatcat tcagatatct ttgaaacaat
    11161 ccgtacaaca aaagatcttc cagaagaaga agcgttgaac agtgcgattc aggaatataa
    11221 agacatgttc ttagctacaa aaggaaataa ttcctctaca gaagataaat tgaaatccat
    11281 tcaaaatgca tagtgaggtg agatgaatgg gtgcttcatt aaatgaaatc aagacacgta
    11341 tcgcttcaac gaaaaaaacg agccaaatca cgcgggcaat gcagatggtg tctgcttcta
    11401 agttgacaaa atctgaagct tcttcacaga agtttcaggt ttatgcaaca aaagtaagag
    11461 aaatcgttac gcatttgact gctactcagt taaatgatat tgcctcagaa aatcctagag
    11521 gggatatcaa ttacaacagc atgttgctca gccgtcctgt taagaaaacg ggttatatcg
    11581 tcatcactgc agatggcggt ttagtcggtg gatataacag ctcgatattg aaacaaacga
    11641 tgtctatttt agaagaagac caccaatcac ccgatgatta cgtgatgatc gcaattggcg
    11701 gaactggtgc agacttcttt aaagcacgcg gtatcaatct agcatatgag ctgcgcaatc
    11761 tatctgatca gccaagcttt gatgaagtcc gcaaaatcgt aaatatggca acaactatgt
    11821 accaaaacga agtattcgat gaattatacg tatgttataa ccatcatatc aattcgctaa
    11881 ctagtcagtt tcgtgtagaa aaaatgctgc caatctcaga cttagatcca gaagaagcaa
    11941 ctactttcga acaagaatat atttttgaac cttcaaaaga agaaatcttg tctcagttat
    12001 tgcctcagta tgctgaaagt ttgatctacg gtgcgatcgt tgacgccaaa acagcagaac
    12061 atgctgctgg tatgacagca atgaaaacag caactgataa tgctgctaat atcattgatg
    12121 agctgacggt ttcttacaac cgtgcaagac aaggggctat tacccaagaa attaccgaaa
    12181 ttgttgccgg agcttcggca ctagaatagt attgagattg gaggaaaaaa gatgagttca
    12241 ggcaagattg ttcaagtaat cggtcccgtt gtcgacgtgg aattttcttt agaccaatcc
    12301 ttaccagata tcaacaacgc attagttgtt tataaaaatg acgaaaataa atcaaaagtt
    12361 gttcttgaag cagcgttaga attaggggac ggagtgatcc ggaccattgc gatggaatca
    12421 acagatggtt tacaaagagg aatggaagtc attgatacag gcaaagcaat ctctgttcct
    12481 gtaggtaaag aaacattagg tcgcgtattc aacgtactag gagatacgat cgatttagaa
    12541 acacctttcc cagaagatgc ggaaagaagc gaaattcata aaaaagcacc agcctttgac
    12601 gaattaagta caagtacaga aattttggaa acagggatca aagttatcga tttgcttgcc
    12661 ccatatttaa aaggtgggaa agtcggacta ttcggtggtg ccggtgttgg taaaaccgta
    12721 ctgatccaag aactgatcca taatatcgcc caagaacatg gtggtatttc tgtatttacc
    12781 ggtgtaggtg aacgtactcg tgaaggtaat gacttgtatt atgaaatgaa agattcagga
    12841 gttatcgaaa aaacagccat ggtgttcgga caaatgaacg aaccaccagg tgcacgtatg
    12901 cgtgttgctt tgactggttt gacgattgcg gaatatttcc gtgatgtaga aggtcaagat
    12961 gtactgttgt ttatcgacaa cattttccgt ttcactcaag ctggatctga agtatcagcc
    13021 ttgttaggac ggatgccttc tgcggttggt tatcaaccaa cattggcaac agaaatgggt
    13081 caattgcaag aacgtatcac atctacgaaa aaaggttcga tcacatcgat ccaagcaatc
    13141 tatgtgccag ccgatgacta cactgacccg gcgccggcaa cagcattcgc ccatttggat
    13201 gcgacaacca acttggaacg taaattaaca gaacaaggga tttatccagc ggtagatcca
    13261 ttggcttctt cttcaagtgc tttagcacct gaaatcgtgg gagaagaaca ttataaagta
    13321 gcaacagaag tccaacacgt attgcaacga tatcgtgaat tacaagatat cattgctatc
    13381 ttaggtatgg atgaattatc cgatcaagaa aaagtgttag tttctcgtgc ccgcagagtc
    13441 caattcttct tgtcacaaaa ctttaacgtg gctgaacaat ttactggtct tccaggttca
    13501 tatgtccctg tagaagaaac agtgaaagga ttccgcgaaa tcttggaagg caaatacgat
    13561 gatcttcctg aagaagcttt ccgaagtgtt ggccgaatcg aagacgtcgt agaaaaagcg
    13621 aaaacactag gctactagaa aggggtgccc catggatcaa tatttaacag taaatgtggt
    13681 aactcctgcc ggtttggtct atgatcacca tgcagctatc gtcgttgcac gtacgacagc
    13741 tggtgagctt gggattttgc ctaaacatgc accaatcatc gttccattga cgattgatga
    13801 agttcgtgta aaacgaacag attcagatac ccacgtcgat tggatcgctg ttaatggcgg
    13861 aatcatggaa gttcgtgata atgttgtttc aatcgttgct gatagtgcgg aacgtgaacg
    13921 tgatatcgat gtatctcgtg cggaacgtgc aaaacaacgc gcagaacgcc aaatcgaaga
    13981 agcaaaagca aaacaagata cgaacgaatt gaaacgggca actgttgcgt tgcaccgagc
    14041 aatcaatcgt atcaaagtat ctaagcactc ataaaatgag atgcggatgt ttatccgtgt
    14101 ttaagaacag taaaaagagg ttatgacaga agtggtcagc ttcaagacat aagaaggaat
    14161 ttacgaaaat tgcttctcaa atttttgtga aatctcagct tatttttcga agaagctact
    14221 tctggaacac cgtatatcgg aggttgtgaa atcagcgttt tgtcctgagt attagagaaa
    14281 caaatggaaa atagttcctc atattttgcc gttttttgag ctaataccga gggtcagatg
    14341 attgaacacc gtttatttgg aataagggac tgcgacaaga cttttgggct ctttgtcaat
    14401 aaggactgat gagttgtgca aaatcaaatc tgagtcagaa tgaaccacat tctggctcag
    14461 att
//
LOCUS       DACDTH010000069        14368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDTH010000069 DACDTH010000000
VERSION     DACDTH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.069"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            83..1081
                     /locus_tag="IWK54_002290"
     CDS             83..1081
                     /locus_tag="IWK54_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9594389.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            1105..1389
                     /locus_tag="IWK54_002291"
     CDS             1105..1389
                     /locus_tag="IWK54_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594390.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            1470..1868
                     /locus_tag="IWK54_002292"
     CDS             1470..1868
                     /locus_tag="IWK54_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9594391.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            1878..4421
                     /locus_tag="IWK54_002293"
     CDS             1878..4421
                     /locus_tag="IWK54_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP9594392.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            4443..6785
                     /locus_tag="IWK54_002294"
     CDS             4443..6785
                     /locus_tag="IWK54_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594393.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            6801..8975
                     /locus_tag="IWK54_002295"
     CDS             6801..8975
                     /locus_tag="IWK54_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP9594394.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            8965..9387
                     /locus_tag="IWK54_002296"
     CDS             8965..9387
                     /locus_tag="IWK54_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9594395.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            10330..11241
                     /locus_tag="IWK54_002297"
     CDS             10330..11241
                     /locus_tag="IWK54_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594396.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            11728..11994
                     /locus_tag="IWK54_002298"
     CDS             11728..11994
                     /locus_tag="IWK54_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594397.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            12066..12407
                     /locus_tag="IWK54_002299"
     CDS             12066..12407
                     /locus_tag="IWK54_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594398.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            12462..12881
                     /gene="ssb"
                     /locus_tag="IWK54_002300"
     CDS             12462..12881
                     /gene="ssb"
                     /locus_tag="IWK54_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9594399.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            12958..13254
                     /locus_tag="IWK54_002301"
     CDS             12958..13254
                     /locus_tag="IWK54_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594400.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            13269..13700
                     /locus_tag="IWK54_002302"
     CDS             13269..13700
                     /locus_tag="IWK54_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594401.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            13882..14187
                     /locus_tag="IWK54_002303"
     CDS             13882..14187
                     /locus_tag="IWK54_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594402.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         5069 a         2229 c         3047 g         4023 t
ORIGIN      
        1 gagggggcaa gtgtggttgt ccacgaagta gtgattgtgg acaaaatacc gaaattattg
       61 ttagaacggt atggcatttc tcatgagtaa tgcaaaattt aatccacaag cgttccaaac
      121 taagccaccg aaacaacaga aggaaaagaa acaaaagaaa cgttttttgt tccaaaaaaa
      181 gaaaacagaa ccgtcagatc gaatgatcca attttcgcaa aaaagtgcca tgagtgtatt
      241 ttcaggcgct tttttttgtt tgtttctgtt aatgggtgtc gtgctcctcc taaactttgg
      301 acgagtggat acattaacgc gtttagcagt gaaaaaacaa gtcaatccag aaacattgtt
      361 aactgaagtc aaccaaacct tgtcttctac ggaacaattg aagtacgagg gacaacattt
      421 tattgaaagg ttatttactt attcgccaga aaaacgaaac gaatgggagg cttccgtcaa
      481 tcattattta gcacaaggat tggaagcgtc tgatctaggc tttgtaacga cggctaccaa
      541 tcgagtagcg aaagatgttc gctttattaa actagaagaa atcgatttga cacaagcaat
      601 gtatcgccta ttttatgagg ttacttttac agaaggagaa cattggaaaa gcagtcaact
      661 ttcttttcca gtcagttatc aaaacaatgt tttaaaagta cttgatggcg ttcaattttt
      721 gaatctttct gagagtaaga ataccaattc tgtgaactat caggaaaatc gattttattc
      781 ttctgggcaa gcggtcactg aaagcgaaga aatcaagctg gaagcatttg tgaaacggtt
      841 ttttgagtta tatgtcaaaa atgatgaaaa attggcattg atttctaacg aaaaagggtt
      901 agaaggcggc gaattgatca atgtacaact ggatcatgca gtaaaaaata aagaaggaaa
      961 ctactttttg caagggaagt atcgtttcta ttttgagaaa ggaaacgagt tccaatcaaa
     1021 cttttcaatg gaagtaaaaa caagtggcga tagttatttt gttactacgt taaatgaata
     1081 aaatgaactt agaggagaat ggaaatgaaa ctttttgatc ttaaatcgtt atttgatgat
     1141 gtatctggac aagtcatttg gctattggca atcgttgcga ttgttctaat ggttggtgct
     1201 tatgcgaccc aaggctttgc gcgggcagcg tttgccatta ttggcgtctt tattttgata
     1261 atgctcgtct tattgttatc gaatgccgtt gatattggtg aatggctaaa gaacattatc
     1321 tttattaagg atagtgaaga gaacgtgggg gtaacgcaaa tgattgtgcc ctggatacaa
     1381 ttatcataag aaaaagacaa actgcaaaag cgatcagaag attagaactt ctggtcgctt
     1441 ctttttttgt aaaaggaggt agagtgaaca tggaatacaa ctatacaaag gagtttaagc
     1501 accctcacaa aatttacggg attaaaggac aaaaaattcc ctggatttct aatggcattc
     1561 gcatggaaca attagtcata ggtggggtat ttctcttcat tcttttgatc ctatttttga
     1621 ttgcgtatgt gaatcaaatt agtttcttgc agacactgtt tcgaaagtcc tggttgatta
     1681 tgatttttgg gattggtgtt ctcgtttgga cattgttttc aatgaaatgg gacaataaac
     1741 actttttcga gtatttaatt gggcgaacgt cctattatca gaaacggaaa caacgctttg
     1801 aacatgaaat gtatgtgccg tttttccatg aaaaagtgac gtattcccta cacaaatgga
     1861 aggagtgagt cgagtaaatg gcgtttttgt ttcttttggt cgtctttatt atcgggattt
     1921 ggcagttcaa aaagaaaaaa acgaagcttc ctcgattaga atttccgata gacgccatta
     1981 tccgaaattt tgttttgacc aaaaatgatg atgtgtgggt aggctatcaa ctttcttccc
     2041 aagtatttcc tttaaatgac attgattttt tcaagaacta tcttcaagat ggcgaaggca
     2101 tacttggaca tgatgaatat gactatcatt ttatcaatat tccacgtcaa tttaacctag
     2161 atacgcacat tgaagaaacc attcgagatt tggtaaaggg agaatgcgca gatcttggga
     2221 aagtgtactt tgaacaggca ggaaagattt taaaagacga agtgcaatta aacgagtatc
     2281 gaacgtatct gtttgtgcgt ttcactacag aaatgaaagt gtctgatccg atcgaatatt
     2341 ttcaattgct taagcatgtt tttaaaaagt ttatctatcg tattacggga caacgagttc
     2401 ctataagtag tgtgttacca acgtatgaaa cattagaaaa ggagctctat acggaattgt
     2461 ccaattataa gccgatagag cgcttatctg cccaagagat tggacaaatt tattattatt
     2521 ttttccatcg atcagaagaa aaattgcctg aaagacagct tacgccaatg gaaatttgcg
     2581 agggcgtcat ttcgaatgaa gcaggataca ttgcgattga acagttgaat caaacgcatt
     2641 attcgacgtt tttaagtcaa acggacgctc ctttagccat gtatgggagt agctttattc
     2701 aaaatcttca ggatagctta tcttttccaa ttgatacgca tattcgactt cgatttgagc
     2761 attcaaaatc tgatgaacga aaaattaata aaatggctgg acggatttat gaacaagaaa
     2821 aagaatatga gaccgttgac ggcattttag attcagacga tgtattaaat tttggtacag
     2881 aacggttaat ggatgtaaaa gataagctga agcgcaaaga aaaacgattg tgttattcta
     2941 ccgtcacgct ggttgtatct gcttcttcaa aagaagaatt ggaagaacga gtcaaagata
     3001 ttgaatttgc gattgagtct acagatttca aattttatcg atcggtagcg gatcagctaa
     3061 cgttgtttaa tcaatgctta attggaagta cgcaacgttt tcgtagttac gagcaggtcg
     3121 ctacgacagg ttttgtggca gattatggct tggacttaaa gaaagaagta ggaaataaga
     3181 aaggatttcc tttaggacgg attgttattg caaaaaagtt taaatctgcg aaagaagcgt
     3241 tagcgctatc tagtaaacta gtatggtatt atcccaattt aacaaagaaa agtattgaag
     3301 gcgcggaaca taccaatgga aatacgttga ttattggtcc accaggtaaa ggaaaatcgg
     3361 tccttgtaaa atatattttt ttatggctga tgtttttagg tcaaaaaatt ctttatattg
     3421 atccgaaaaa tgaaacccaa aagttctttt taaaggcact tgaaaaattc ggacacatac
     3481 cagagtttgt ggctatgtat gaacgaatta attttatttc gttatcttca gatgaaagct
     3541 gtcgagggat gcttgatcct ctattgtttt taccaaaaga agaggcaatt caaaccgcta
     3601 aaaatgtgtt aggaatgtta ggagaagtag ataccaatcg agaaacagcg agccacaaga
     3661 aaacggtgat ccaagacgcc atcgatgtag tttacaccat gccaggtaag cattcgttaa
     3721 gtcaagttat cgaagaaatt cgaaaaagtg acaaagaact agctaaattg attttagggc
     3781 atagcgttgg tttaggaaag attctcattg ggaatgagca cagtacgccg atacgttttg
     3841 attcacaaat caacgtgtta gggacacaag gaatacgcat tccgacacaa aaagagattg
     3901 attcgggtcg attaaatagc gaacaacttg caggaatggc aattatggaa gtcattatga
     3961 agtttacgta tatcttttct acaaacaaag aagaagacgc tgcgattatt tatgacgagg
     4021 caaaagggta tgaagatacc gcacaaggaa gctatttgat tgatgatagc ttgcgaaaag
     4081 gacgtgcgaa taatacggat atttaccttg ttgtgcaagc gtttaaagat tacgatcaag
     4141 aagataaaaa agagttaatt tcttacaaat ttgcgtttcg tccgaaacaa aaagaagcac
     4201 gagagaaact attgtccttc tttgatatgg aagtgaattc agccaattta gaaatgatgg
     4261 aacaattaca aacaggaacc tgtttattcc aggatcattt gggaagaaat caaccgattg
     4321 ccattgatgt gttgtttgat agttggttat tggctgtttc ttctactgaa aatcaagatg
     4381 aaaaaattca agcaatgtta gaaaatgaaa aacaacgata aaacaaaaaa gaggtggttt
     4441 ttttgcgaaa aaaatctctg aaatggtgga cgctcctttt attagggggt ctttttttct
     4501 gtctacaagg ggctactgtt tctgccgatc aaatgaatga tggtgcgggt gtcactgaaa
     4561 ccgatcgttc tgaaggagag aatgccaacg ataaagtaga agtaaaagaa gtaacgactg
     4621 atgtggatca attgtataaa aattacaaaa agaattcgtt tgaattgatg accaatgact
     4681 atgagtcaat gaaccttaaa aaaaagctat cgtacaatgt tgcaggagtg atgaaaaatt
     4741 ttgtttggag tggcgtaaaa atgttaggag aattcaatgc ttcaatggtt gaatttctct
     4801 ttgaattaga tattatttcc gccattcgag aacccattca aaacttaaca caaaaaattg
     4861 cggacaatat gttaagtatt gcaggaacta ttggaatcag tttttcggca attatgatgg
     4921 caatcaaata tgctggagag gctcgaatga aacaagcact ccgtgtcttt cttatgacga
     4981 tccttatttt tactgggtta agcgtattaa aagattctgg gacaaataag aatctttttg
     5041 atgttctttt ttctttagat tctacagtgg aaacggcgtt cgtgaaggtc aatccagtgt
     5101 taacacctga agaagaaaca aattatgaaa caaaagaaac agaagaggta acagcggata
     5161 aaaaagtgga aacggcaggc aaattgatgg cggctcgggt cttttatagt aatgtttatg
     5221 aaccatatct gttgttaaat tatggcacaa ccaatgtaga cacaattcgt gagaagaaag
     5281 tcaaatatgc tggaaaagaa tatgatcgaa tcaatctttt attggacaat gatttaggta
     5341 caaaaatagg ggagaaaatt catgacaaag tcacagagta tgaagcagat acactgaaaa
     5401 acaagaacat acaatattac aatagttgga aaaatacagc atttgccttg ttttattttg
     5461 tcgtgaatct gattcaaacc gttgtttatt ttgtcttaag tatgtttcga ttgattattg
     5521 gaactatgca actattgtta gtaccgatgt tgccgttctt acttttttta ggattgtttc
     5581 ttgtcacaat gaatgtgttt ggtaactatg ccaaagtttt tggtattttg acgtttttaa
     5641 aaggaatgac aggttttgca tgtatccttt tttcttcttt tcttagttac gggttccaat
     5701 taagtagtac acaagacgat ccatgggaaa aaattctctt gatcattgtt tatttgtttg
     5761 cgccgttggg aatttatttg ttccgaggct ttttaggcag tctatttact ggaagaatgg
     5821 gtgttcgagg agcgttagct tttgcgggaa atccatttgg ggcagaagca aaattgagaa
     5881 gggaagccaa acaacggttg aaagaacgca aagaagcgag aaaagcacag aaacaaaaag
     5941 aaaaagaaaa cgcacggaag agacaagaac gtcaacgaaa acgtgggggt ggctcagacg
     6001 ttcgtatgcc acaaacggcg aaacaagcca cacgaagtgc ttttcggcgt gaatccgcac
     6061 aacaacctcg tcataaagca ccaaatcgta tggaacgtat ggcacaaaaa gcaagagact
     6121 tgcatgatca agcgaaacaa gcagaagagc aaagtcaaga acgtatgcaa cgaacagcaa
     6181 tggagaaaca ggcaagaaaa acagccgatt tattacaacg agaattgcaa ggcaatccta
     6241 gtctaagaga aagaagtaga aaaggtgcgt ctcgtagtcc gtatcgcaat cagggttctg
     6301 caatggtggt gtcattagcg aatccaaagc aaaaacacaa gtcttatggc cagaagcata
     6361 cgcctagaac agaattgaac gatcgaaaac gtcgtcaggg acaaacaagt cgtcgtcgga
     6421 aggaggcact tactcccgta acaaaaacaa gttcgatgaa aacccggaca gttgcaggac
     6481 aaaaacatgc ggtacaaaaa ccactaagaa aagggaatgt accacgagca acaggttcta
     6541 ccacaccgaa atctgttcga cgtccagagg gggttcggga acatatgtca gcagtcaatc
     6601 gagtaacagc acaaaccaaa gcgccttcaa ccgtatctaa agcatactca accatgaaac
     6661 aaacaaaatc accaaaacaa gcaccgaaga cacggagtga gtttcgagca actcaagcaa
     6721 aaatgaaacg aaaacgagaa aaacaaccga tttatcgaag agaaatgaaa caacgaaaac
     6781 gataggagga aatcgatagt atgatgaatg caccaaaacg accgacacat tttgagcaaa
     6841 tgttttctgt cgatcgcaaa agaaaacaag aaaaagaaac gccaaaaagt cggtttcaac
     6901 aactttattt tatcgtcact ttattaggtg tttgtctata tccatttttt attgtaggca
     6961 gtattccttt agcgattgtc tttatgatag ataaaaaaga gacacgtgca catatttatg
     7021 actttgagta tgagagcttc ttaaaacgaa gaagctctct tttttttatt tttgcgttag
     7081 gttttacgct attaaatcta ttttgtttta tttttgttct tcctagaggg tatttgtcgt
     7141 gttacttatt atttccatta aacttgtttt ctcatgcgtt gcagtttaat ggacaaacga
     7201 tttttgccct ccttttaggt gggacaggtg caggaagttg tttgattgcg tatgcgtctt
     7261 ttattgaaaa aagaaaagtt gtttcaaaga aagaacggca aacagctttg ctaaatagta
     7321 aagaatacaa ggaacgtcgg aagaataaat ttcaggagtc ccaaaaatat acagcagagt
     7381 atcagcaagc gtaccaggta gcaattgtca taaaagacgt tgctttgcgg aataaacaac
     7441 tggcttcatt agccaaagtg attctattag gaacagatga gtatgggctc ccttatgtga
     7501 tgaactttag tgaattgaac caacatgtga ttattccagc gactacaggt tcaggaaaaa
     7561 cgacggtgat ccaattacta gcagaacatg cgtgtaagtt tcgtatcccc gttatcttag
     7621 tcgatgggaa aggagcagga gagactttac aagcgttaaa agaagtggca gaggcataca
     7681 atcgcccgat tgaagtcttt gtggacggtg gcgatttgcg ttataatcct gtaaaaaatg
     7741 gcaatgatat tgccattcga gataaattaa ccaatctagc agaaacagaa agcgtgtatt
     7801 attcgacagc agccaaagcg ttattacagg taacgattca gcttttagac gagttccaaa
     7861 agagccataa aattgaaaga aatctagttt ggttacaaca ttatttattg ccacgaaacg
     7921 tcttacattt gtttgcggat cgtattcagt tgaaaaatcc agatttattt aaagaaaaag
     7981 tggtcgtaga aaagaagaaa accacgaaga aaaaaacgaa aaagaatgaa gcgatggaca
     8041 aaacagaacg tgattttgcc gatgtggaac agttaagtga agagcgaatc gttgaacatg
     8101 atcgtaaaac cgttgactta gaaacgtatt atctattatt aaaagaaaac atttctctca
     8161 tgacagaaat cgagcaaacg ttgtttaaac ggttgtttgt tcgttacgaa cataagaaga
     8221 acccgtttta tttgtatgcg acttctgaga atttacagac caatatcaat atgttattgg
     8281 atagtgagct tggtgaactc tttgatactt ctggaggaaa aagagaatta gatattcaag
     8341 aagttgttaa agaaggaaag attgtttata tctcgttaaa cggattgatt tatagtgagt
     8401 ttattcggac attggctcaa atgcttgtgg gagatattaa tttctttgcg tcagaagtgt
     8461 ataaacaagc aggagaaaca gccaatcgct cgaaagtcct agtaatattt gacgaaccag
     8521 cttcttatct aaatgataac cttatagact tagtcaataa gggacgaggc gctggggtgc
     8581 atgcggcgta tgcccctcaa acgcttgcgg acattgatag aattgatcct gtccttcgaa
     8641 aacagttgat tgggaatgtg aatacgttct ttattgggaa gacaaatgac ccgacagaaa
     8701 ttgattattg ggcagattta atggggacag aagaagacat tgaattaacg gaaatgacga
     8761 cacaagaagc aggttattct gatatgggaa aggttgattg gacaggagaa agaggcacga
     8821 aacgagacgt agaccgcttt aaattcaatc cgaatcgcat tcgagcattg agaacaggcg
     8881 aatttgtggt gtatcgaaca gctgaagacg tccaggaacc accgcgagtc gtctatattc
     8941 ggaaaccaaa tttggagaaa caatatgcga aataagaaag ttattgttag gatacttatt
     9001 gttagtttcc tcttatttgg ttgttttaaa ctgataaaaa ctaacgaaga atcaaatgga
     9061 aaactaatca atagtaattt agaagaagta ctagaacttc aaaaaacgaa tcaatcattt
     9121 tttgtgtatc tagcaagaaa tgattgtaaa aaatgccaag aagttgaatc tttcttggaa
     9181 agatacgaca aaaaattctc taggaatgta tatcgaattg aaacaagaaa agaaccaaga
     9241 caagaagaac ttcaaacttt tttacaacgt taccatgtta aaagtgtccc aagttttctc
     9301 gtgattcatg atagaaacgt caaaaaaatt gagagaaaaa cattcttttt tgaaaagaca
     9361 ttgaatcaat ttcttcctaa tttttagtga aaattgaaag agaatacgtt gaaaaaaaca
     9421 aaaaattgtt atgagttcaa atttttttcg atcgtatttg tttttctttt gaaacaaatt
     9481 cactcatttt ttgagcaaaa atgtttcaaa atttgaacaa aaaccaattg aaaattgaag
     9541 caaatttcac tcgaattgaa tcgattttga gctaaaatca atagaaaaaa taaacgtatt
     9601 ttgtttgaaa tccgtttatt ttttttacgt ttcagtatta gtaaggatta tttgagggtt
     9661 atttagagat tatttaaagg ttatatgtaa taagtggggg gagggctgtc ggcgcttggt
     9721 atgaaaggca ttctccgact tccaggtttt tgaccatttt ctctcaacgt ggaaaaaccg
     9781 ccacttatga gagaaaatgg tcaaaaacct aagcgggaag tctccgaata acataccaag
     9841 cgccgacagc caacagataa agaatacact actaaaaata gtagtagcat aaaatttaaa
     9901 agggaaaaaa tcaaccccta ccccagtctt ggggtctcaa aactaactcc cccctaaccc
     9961 cctctttcag cctcttttga aagaggggga gaatcaaccc cacggccaac gccccagtgt
    10021 tcttggggct gtccagaaca gaaacttgcg agagagtggg ggtaaaacaa gtgtagagga
    10081 aacttgaaga cttgttcgaa cagaaacttg cgagagagcc ggagtaaaac aagtgtagag
    10141 gaaacttgaa gacttgttcg aacagaaact tgcgagagag ttggagtaaa acaagtgtag
    10201 aggaaacttg aagacttgtt cgaacacaaa cttacaagag aggggagtaa agcagttgca
    10261 gaggaatcag gaagatttgt tcgaacagaa acttgcaaga aagcattatt aaattgaagg
    10321 tgatgaaata tgaagtattt attgcatggc tattttattg attattctac tcgcaatagt
    10381 agtactttag cgattctatg tttgcttcat gaattgagag tggttactag aaaacagcta
    10441 tacaaactat tgaattttga cagatcaatt agtaaacaag gagtaacgaa tgctttagtg
    10501 agattatatg aaaaacagtt aatcgatcga aaaaaatatg gaacagaaat gtattattat
    10561 ttgacgaaag agggacatca aagcataggt gggtattata cgttgccgaa agttcctgaa
    10621 tataatttga atcaccattt acaaattaac aattatttga ttaaaggaat ggcattggca
    10681 aaagaccacc cacatttaaa acggattatt tcagaaagac gtaccgtcta tgaaacgaag
    10741 gatttaaatc ctaagacaaa aggaagaaaa ttttttgtgc cagattttat ttttcgtttt
    10801 cgagacaaag aaaatcatga gatcaattgg tcctttgaaa ttgagctaac gttgaaaaca
    10861 cgtcgtcgct accgtgaagc gatttttccg aaatatattc gtgagttgcg aacacgtcct
    10921 gaatcacatt taatttatgt gacacctagt ggcgcaattc aagaagaact tcatgcgttt
    10981 aagcgtattt ttttcaagcg cgaaactgaa aaagatgcga gtcaacaaga attgtttgag
    11041 cggttgcata ttttttcagc tttagagttt gaagagggat taaaagcatt attagagaat
    11101 gatccagcag tcaattggcg gacagtagaa cgagaaaaaa tttatccgaa tggcgctgtt
    11161 caaagtcgtt tgaatttatt tgaagaatca actgaaaatg aggaaaaacc aaaagtgaag
    11221 tttacgcctg aagtattgta aaagagggtg ttcgcttcgc tctttttttg tgccagtttg
    11281 ctggcaccgt tcagacgcaa gcgtctgaac cggctcgttt ttaggaagga ctaacttgtt
    11341 tgatttatcc gttgtcaaag aagtggacgt tcgagagtcc ctaatcgtat agcagtcatt
    11401 cggcgagtat aacctaaaac ataacctgcc aattgccaag ggtaaaatgc acgctgcgcg
    11461 cccttggcaa tcgtcagaac atgttttcga gccggttatg ccgaacgacc gctatacgaa
    11521 aagggcctct aaacagaagt ccactttgat ccttgacaac tgaaacccaa aatcaaacaa
    11581 gcaggccttc gtctaaaaac gagccgaatg aagaccttgg gggttgcacc cccaaacccc
    11641 cgtgcccccg acacactacc gaaaaggtgg gggtgggtgg cgaaaaaatc aaataaaaca
    11701 ttctattcaa gaaaggaaga aacaaccatg acaactaaaa acgagcaact gaaagaacag
    11761 gcaagaaaag agttgcaaga aagaggtttg attattgagg ggagctttga aggagatttt
    11821 gaaacctata ttggttgtta tgcaagaccg ataaacaaac ctacagcctt agacccaatg
    11881 aacgaacaag aagtaaaaga acaagaaaaa catgcagtga atggttttcc gcaagatttc
    11941 actgaatggt atgagtggga aattaacaac gggaagcttg aaaacttctt ataaaagtca
    12001 gatgacgtgc aaagagaatg attctctttg tacggtaaaa atataaagaa agaaggaaaa
    12061 agaaaatggc aaaaaacaca ccttatcaag taggagacat tttttatagt agttggggct
    12121 acgaacaaac gaacgtgact ttttggcaaa ttgtgaagtt aacagaaaaa acagcatggt
    12181 ttcagcctat tcaaaagaaa acggtcgagc attattcgtc catgagtgga aaagttgttc
    12241 caatgaaaga caagtttata gagtatcctt tagcaaaaac taaagacagt attgtaaaaa
    12301 aacgaattga tccagaaacc tatcctgata tgttatgtgg ttatcgtagt tgggacacgt
    12361 ttcatagata taaaaatcaa gctgtttatg aaagtagtta ttattaaggg gatagtttcc
    12421 cctttcaaaa ataataaata gaagaatgga gagaaaaaat catgatgaat tcagtgaatt
    12481 tagtaggacg gttaacaaaa gaagtggatt taaaatacac accaaaaggc aatgcgacag
    12541 gcacatttat tttagctgtt aatcgaaatt atacgaatgc aaacggcgag cgtgaagcag
    12601 attatatccg ctgtgtcatt tggcggaaag ctgcggaaac tttagcaaaa tatactggaa
    12661 aaggaagttt gattggcatt aatggacgtc ttcaaacgag gagctatcag aatcagcaag
    12721 gacagaccat gtatgttacg gaagttctag tgacagattt ctatttgtta gagagtaaag
    12781 aaatcaatga gcaacggtca aaatcaatgg cgaataatca aaataattct tcaattccag
    12841 gaaatgagat tcaaatttca gatagttatt tgccctttta agtgaaaaat aattttgttc
    12901 tatacctgat ttcttcaggt atagaacaaa aaataaatag agatgaggcg attttttatg
    12961 aaaatcattg acagttacga tcaacgaacc atgcaatatt tgggctcgtt tgattataca
    13021 gatgaaaata ttattaatta tgttgcaagt ttacctcctt atcaaagcgt tcgcttggta
    13081 gattttgcaa gtgatgaatt ggtattaaca acgatgggca actttttaga ttatgtcgga
    13141 gacaaaaaat ggttggaaaa aattttgtct aaattgattg cgaaacaaac aggagaagaa
    13201 cccataaatg cgattcaatt gttcaatcga tatgaacaag aaaacaatga ttaaagagga
    13261 gagaaaaaat gaaaagatta aaacgaagta tttttattgt gctagcattg attgattttg
    13321 ttgggacagg ttgtgaagca attgacgttc aaaaagctcc cttatcggaa ataaaacaag
    13381 caataacaaa acaagatgaa gaacaaccgc taaatacaca agtagaaata gtgaatcaaa
    13441 ataaaccgag tttttctgaa gaagattttt cgttagaaaa tggaaccagg caattttttt
    13501 cagatttgga tcgtttaaat cgggtaggtg tagcaagtgc acttttgcat aaaagcagga
    13561 tgccaacaga agaacgagac cgattataca ttaagccaac tggctggaaa caaaagaaaa
    13621 tgaagaatgg cgattatctt tataatcgtt gtcatttgat tggctatcaa cgtgcgacaa
    13681 gtttccccat aagtatgtaa tacgaaacgg gaggattaat cttaagatta atcacaacta
    13741 acctatttga cagttggaat gaaagctatc atgtctgctg aatcaactgg tctgatactc
    13801 ctacatgcaa taaagtctac taggaagtta ttgtatgaag ctaggtaaag gcggcagaac
    13861 aatttctaag tgtggaatca tatgaagttg gatatgccgg gacgctataa attactcagg
    13921 gtgagactgt ttttaattaa actgacgaac ttgcgaatgt acgggtctat aaccgactta
    13981 tattcgaaag atataagatt gcgaatgcaa agaacattat cgaaaagtaa gaatgttggt
    14041 catgaaattc gagatggaag actagcggtg tatctgtcaa gagttggtaa aacatgtttg
    14101 attgattcag atgagagtgg gaatgcgaca aaacgtttta cagaagaaat agagcaacta
    14161 gcagaactga atatacgata tttttaaata ctttagcgga gaataaaaaa atcctctaca
    14221 gaaaactacg gagggcaaca tgtgctattg tttcaacaat acatttttaa tttttaccat
    14281 aacaaaaaag tacttgaaaa aaaacagaaa tatgttaaat taaaaatgaa aaaatggaag
    14341 cggttactta ttcttttgaa tctaagat
//
LOCUS       DACDTH010000070        14202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDTH010000070 DACDTH010000000
VERSION     DACDTH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.070"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(221..1423)
                     /locus_tag="IWK54_002304"
     CDS             complement(221..1423)
                     /locus_tag="IWK54_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9594403.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="IWK54_002305"
     CDS             complement(1426..1620)
                     /locus_tag="IWK54_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9594404.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="IWK54_002306"
     CDS             complement(1667..2515)
                     /locus_tag="IWK54_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP9594405.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="IWK54_002307"
     CDS             complement(2720..3070)
                     /locus_tag="IWK54_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594406.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="IWK54_002308"
     CDS             3665..4111
                     /locus_tag="IWK54_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9594407.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="IWK54_002309"
     CDS             4735..5727
                     /locus_tag="IWK54_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9594408.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="IWK54_002310"
     CDS             5743..6627
                     /locus_tag="IWK54_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9594409.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="IWK54_002311"
     CDS             6676..8283
                     /locus_tag="IWK54_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9594410.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="IWK54_002312"
     CDS             8284..9768
                     /locus_tag="IWK54_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9594411.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="IWK54_002313"
     CDS             9945..10937
                     /locus_tag="IWK54_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9594412.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="IWK54_002314"
     CDS             10989..11858
                     /locus_tag="IWK54_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9594413.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="IWK54_002315"
     CDS             complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="IWK54_002315"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9594414.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(13898..>14202)
                     /locus_tag="IWK54_002316"
     CDS             complement(13898..>14202)
                     /locus_tag="IWK54_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9594415.1"
                     /translation="TAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFH
                     RYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         4345 a         2408 c         2935 g         4514 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt tggctcttcg tcaaatcgtg ttggtgaaga caaatttgaa taaaacttag
    13861 tccgatatgc ggcgcgttct gtgcgccgca tattttttta gttttcaaag atcagacctt
    13921 gttgtaaata gatccgtgcc cgaaagttat aaaagttgcg ataaccaaac gcaattcgtt
    13981 taatcagctt gatcttgtta ttcagcccct cagtgacgcc gtttgagtgg gacaccttaa
    14041 aggcatttga aatacccgat tggtagcgat ggaagatcgt gaatttcttt ttaaacgact
    14101 gaggtaacgt agatgaaatt gttttaaggg tttcttgaaa gccttccgta cctcgtttaa
    14161 cgaaataata gcgtaataat tgacaaaaat gatacgcagt tc
//
LOCUS       DACDTH010000071        13814 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDTH010000071 DACDTH010000000
VERSION     DACDTH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.071"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            99..293
                     /locus_tag="IWK54_002317"
     CDS             99..293
                     /locus_tag="IWK54_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594416.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="IWK54_002318"
     CDS             562..861
                     /locus_tag="IWK54_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594417.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="IWK54_002319"
     CDS             complement(958..1392)
                     /locus_tag="IWK54_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9594418.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="IWK54_002320"
     CDS             complement(1624..2334)
                     /locus_tag="IWK54_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9594419.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="IWK54_002321"
     CDS             complement(2324..3184)
                     /locus_tag="IWK54_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9594420.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="IWK54_002322"
     CDS             complement(3189..3827)
                     /locus_tag="IWK54_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9594421.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="IWK54_002323"
     CDS             complement(3842..4141)
                     /locus_tag="IWK54_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9594422.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="IWK54_002324"
     CDS             complement(4184..5668)
                     /locus_tag="IWK54_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9594423.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="IWK54_002325"
     CDS             complement(5688..6149)
                     /locus_tag="IWK54_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9594424.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IWK54_002326"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IWK54_002326"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9594425.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="IWK54_002327"
     CDS             7452..8222
                     /locus_tag="IWK54_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9594426.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="IWK54_002328"
     CDS             complement(8495..9322)
                     /locus_tag="IWK54_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9594427.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="IWK54_002329"
     CDS             9890..11125
                     /locus_tag="IWK54_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9594428.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="IWK54_002330"
     CDS             complement(12124..12384)
                     /locus_tag="IWK54_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9594429.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="IWK54_002331"
     CDS             complement(12411..13712)
                     /locus_tag="IWK54_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9594430.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4443 a         2827 c         2174 g         4370 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atat
//
LOCUS       DACDTH010000072        13731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDTH010000072 DACDTH010000000
VERSION     DACDTH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.072"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..1452
                     /locus_tag="IWK54_002332"
     CDS             1..1452
                     /locus_tag="IWK54_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9594431.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="IWK54_002333"
     CDS             1801..2433
                     /locus_tag="IWK54_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP9594432.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="IWK54_002334"
     CDS             3021..4460
                     /locus_tag="IWK54_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9594433.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="IWK54_002335"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="IWK54_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5222..5437
                     /locus_tag="IWK54_002336"
     CDS             5222..5437
                     /locus_tag="IWK54_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594434.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="IWK54_002337"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="IWK54_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6097..7008)
                     /locus_tag="IWK54_002338"
     CDS             complement(6097..7008)
                     /locus_tag="IWK54_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9594435.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="IWK54_002339"
     CDS             complement(7027..8031)
                     /locus_tag="IWK54_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9594436.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="IWK54_002340"
     CDS             complement(8028..10151)
                     /locus_tag="IWK54_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9594437.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="IWK54_002341"
     CDS             complement(10156..12603)
                     /locus_tag="IWK54_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9594438.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="IWK54_002342"
     CDS             complement(12590..12979)
                     /locus_tag="IWK54_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9594439.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="IWK54_002343"
     CDS             complement(13039..13542)
                     /locus_tag="IWK54_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9594440.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..>13731)
                     /locus_tag="IWK54_002344"
     CDS             complement(13555..>13731)
                     /locus_tag="IWK54_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594441.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         4221 a         2710 c         2509 g         4291 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt g
//
LOCUS       DACDTH010000073        13712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDTH010000073 DACDTH010000000
VERSION     DACDTH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.073"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            139..477
                     /locus_tag="IWK54_002345"
     CDS             139..477
                     /locus_tag="IWK54_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594442.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="IWK54_002346"
     CDS             complement(682..1257)
                     /locus_tag="IWK54_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9594443.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="IWK54_002347"
     CDS             complement(1989..2570)
                     /locus_tag="IWK54_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9594444.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="IWK54_002348"
     CDS             complement(2753..3379)
                     /locus_tag="IWK54_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9594445.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="IWK54_002349"
     CDS             complement(3401..4732)
                     /locus_tag="IWK54_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9594446.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="IWK54_002350"
     CDS             complement(4887..5288)
                     /locus_tag="IWK54_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9594447.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="IWK54_002351"
     CDS             complement(5374..5580)
                     /locus_tag="IWK54_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594448.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="IWK54_002352"
     CDS             complement(5675..7177)
                     /locus_tag="IWK54_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9594449.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="IWK54_002353"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="IWK54_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9594450.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="IWK54_002354"
     CDS             complement(7748..8365)
                     /locus_tag="IWK54_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594451.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="IWK54_002355"
     CDS             8586..9959
                     /locus_tag="IWK54_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9594452.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="IWK54_002356"
     CDS             10034..11146
                     /locus_tag="IWK54_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9594453.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="IWK54_002357"
     CDS             complement(11218..12855)
                     /locus_tag="IWK54_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9594454.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13023..13631)
                     /locus_tag="IWK54_002358"
     CDS             complement(13023..13631)
                     /locus_tag="IWK54_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9594455.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3701 a         2886 c         2456 g         4669 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    13021 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    13081 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    13141 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    13201 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    13261 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    13321 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    13381 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    13441 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    13501 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    13561 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta
    13621 aatgaattca tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact
    13681 ttatcattta gaaatatttc ataggtaaaa ga
//
LOCUS       DACDTH010000074        13429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDTH010000074 DACDTH010000000
VERSION     DACDTH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.075"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IWK54_002359"
     CDS             complement(<1..914)
                     /locus_tag="IWK54_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9594456.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IWK54_002360"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IWK54_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9594457.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IWK54_002361"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IWK54_002361"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9594458.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IWK54_002362"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IWK54_002362"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9594459.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IWK54_002363"
     CDS             4440..5321
                     /locus_tag="IWK54_002363"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9594460.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IWK54_002364"
     CDS             5318..6034
                     /locus_tag="IWK54_002364"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9594461.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IWK54_002365"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IWK54_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9594462.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IWK54_002366"
     CDS             6751..7752
                     /locus_tag="IWK54_002366"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9594463.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IWK54_002367"
     CDS             7749..9110
                     /locus_tag="IWK54_002367"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9594464.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IWK54_002368"
     CDS             9070..10221
                     /locus_tag="IWK54_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9594465.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IWK54_002369"
     CDS             10196..11365
                     /locus_tag="IWK54_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9594466.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IWK54_002370"
     CDS             11540..12919
                     /locus_tag="IWK54_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9594467.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IWK54_002371"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IWK54_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACDTH010000075        13398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDTH010000075 DACDTH010000000
VERSION     DACDTH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.076"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IWK54_002372"
     CDS             complement(14..706)
                     /locus_tag="IWK54_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9594468.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IWK54_002373"
                     /pseudo
     CDS             791..967
                     /locus_tag="IWK54_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IWK54_002374"
     CDS             complement(1143..1712)
                     /locus_tag="IWK54_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9594469.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IWK54_002375"
     CDS             complement(1786..2109)
                     /locus_tag="IWK54_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594470.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IWK54_002376"
     CDS             complement(2263..3075)
                     /locus_tag="IWK54_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594471.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IWK54_002377"
     CDS             3429..4277
                     /locus_tag="IWK54_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9594472.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IWK54_002378"
     CDS             4422..5363
                     /locus_tag="IWK54_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9594473.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IWK54_002379"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IWK54_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IWK54_002380"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IWK54_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9594474.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="IWK54_002381"
     CDS             complement(9827..12013)
                     /locus_tag="IWK54_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9594475.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IWK54_002382"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IWK54_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9594476.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="IWK54_002383"
     CDS             complement(12235..12675)
                     /locus_tag="IWK54_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9594477.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="IWK54_002384"
     CDS             complement(12749..13288)
                     /locus_tag="IWK54_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9594478.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DACDTH010000076        13281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDTH010000076 DACDTH010000000
VERSION     DACDTH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.077"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            170..1909
                     /gene="yfcC"
                     /locus_tag="IWK54_002385"
     CDS             170..1909
                     /gene="yfcC"
                     /locus_tag="IWK54_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9594479.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2066..3250
                     /gene="iadA"
                     /locus_tag="IWK54_002386"
     CDS             2066..3250
                     /gene="iadA"
                     /locus_tag="IWK54_002386"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9594480.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3361..3564
                     /locus_tag="IWK54_002387"
     CDS             3361..3564
                     /locus_tag="IWK54_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9594481.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3665..4327)
                     /locus_tag="IWK54_002388"
     CDS             complement(3665..4327)
                     /locus_tag="IWK54_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9594482.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4340..5842)
                     /locus_tag="IWK54_002389"
     CDS             complement(4340..5842)
                     /locus_tag="IWK54_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9594483.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6390..8069
                     /locus_tag="IWK54_002390"
     CDS             6390..8069
                     /locus_tag="IWK54_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9594484.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8196..8675
                     /locus_tag="IWK54_002391"
     CDS             8196..8675
                     /locus_tag="IWK54_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9594485.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8894..9574
                     /locus_tag="IWK54_002392"
     CDS             8894..9574
                     /locus_tag="IWK54_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9594486.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9582..10913
                     /locus_tag="IWK54_002393"
     CDS             9582..10913
                     /locus_tag="IWK54_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9594487.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10928..11890
                     /locus_tag="IWK54_002394"
     CDS             10928..11890
                     /locus_tag="IWK54_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9594488.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12001..12339
                     /locus_tag="IWK54_002395"
     CDS             12001..12339
                     /locus_tag="IWK54_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9594489.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12418..13125
                     /locus_tag="IWK54_002396"
     CDS             12418..13125
                     /locus_tag="IWK54_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9594490.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4237 a         2338 c         2911 g         3795 t
ORIGIN      
        1 aaatggaaag agaaaaaaat aatcatacat aggagaaaaa aaacaaaata gaaaaaagtg
       61 attttctaat aaattattca tgtggaaatg tcatttttac tgtataattc atttgattat
      121 ccatttttta ttcatcaaaa ttactgaaaa aagaaaggaa gaaataaatt tgaagaaaac
      181 aaattcttca ggaaaaccga aacgcgactt cactaaatta tcgacacccc atacgtatgt
      241 cattattttt ggtgtagtca tctttgcatg gatactgact tttgtcgttc cagcaggaaa
      301 gttcagcacg caagatattg aatacaaaga tgcaaatggc gaaacaagta ctcgtactgt
      361 tttacgtcaa gattccttcc gttatgctta cgagctggat aagtcgtatg tgtttgatca
      421 attagaagaa ctgcaagatc atccagcaga acgtgaaaaa ctggatgttc cggaaaaagg
      481 attggaaaaa gtcattgctg acggagaaaa aaatcttacg caggaaaaac tagatgaaat
      541 ctccttgaca gatgatgtgc tttacgatca gtacggcgaa aatatctatg atacttctaa
      601 aaaattgcat aaaacagcca aaatatgggg aacagatgat tttggcggct ttggtttctt
      661 aaattttgtt tttgaaggac tagtatctgg agataagtat ggatctgctg tagggatcgc
      721 tgcattgatc cttgttgttg gaggtgcttt tggtattatt atgcgtaccg gagcgatcga
      781 cgcaggtatc tatgcgttta tcagtaaaac taagggactg gagcgtttag ctttaccgct
      841 attattcttt gctttttcat tcggtggcgc tacattcggg atggcagaag aggtaattcc
      901 attctcaatg gtgatggttc catttgtcat tgcgttagga tatgactcga tcgtagcagt
      961 aaccgtgact tatgtcgcat ctcaagtcgg aaatgcgacc tcttggatga gcccgtttag
     1021 cgtagcggtg gctcaaggga ttgctggaat ccccgtgttg tctggtgcga cattccgatt
     1081 gattatgtgg gtagttgtta ctgcattagc cgctggttat atgatgatat atgcagaaaa
     1141 aatccgtaaa aaaccagaaa gttcgttgac ttacaaatca gatgattatt tccgttctca
     1201 tatcaaaaag acatctgatg aaaataaaaa attcatgctt gggcataaat tgatcttatt
     1261 agagatgtta gttgtccttg tctggatcgt ttggggcgtg actcaaaaag gctattacat
     1321 tccagaaatc gcttctcaat tctttgtgat gggattagtt gctggaatca ttgctgttct
     1381 tttcaaatta gatggaatga aagtcaatga catcgcttct tctttccaat ctggtgcagc
     1441 tgatttagct ggtacagcaa tcgtagtagg tatggccaaa gggattcttc ttgtcttggg
     1501 aggatctgat gcatctgttc cttcagcttt gaatacgatt ttgcatggga tcgggacggc
     1561 gttgaccggt gttccagctg tcattggtgc ttgggcgatg tacatcttcc aaagtttgtt
     1621 caacttggtt gttacatcaa attcaggaca agcagctttg actatgccaa tcatggctcc
     1681 actggcagat ttagtaggtg tatccagaca agtagcagtt cttgcttatc agcttggttc
     1741 aggttttatg gacgctttta cacccgtatc agccagcttg attggtgtgc taggtgttgc
     1801 acggatcgaa tgggcaaaat gggcaagatt ccaaatcaag atgcaaggat tcctgtttgt
     1861 tttaggaact attttcatca tgattgccat tgcaataggt ctgcagtagt actggagacc
     1921 tgtaaataaa aaagtaatcc aaacagtaga taaggactcc aagaaccgtc attttacggt
     1981 aaaatcaaag gagtaaagat cctatcgttt ggattatttt ttaagatcct atcatcagtt
     2041 atacttaaag aaagaggaaa aatgtatgaa aatcattcga caaattgaag tgtttgcacc
     2101 agattactta ggaaaaatgg atgtactgat tgccggtggg aagattttgg ctgtagaaga
     2161 acagctagaa ggcggctacg aaggagtgga agtagaagaa ctttcaggtg agggaaaagt
     2221 actaaccccg ggtttcattg actgtcattt ccatatttta ggtggaggag gagaaggcgg
     2281 ttatcaaaat cgaacgccag aagtcacatt gagccaattg actacagctg gtgtaacgac
     2341 agttgtcggt tgtttgggaa cagacggaga aggtagagat atgactgcgt tgatcagcaa
     2401 agcgaaaggt ctggaagcag aaggaatctc cacttatgta tacgaaggtt cgtaccgatt
     2461 gccggtaaaa acagtaacag attctatcat caaagacttt ttgacaattg ataaaattat
     2521 cggaatcgga gaaatcgcag tatccgatca ccgttcttct cagccaagtt ttgaagagtt
     2581 cgctcgtgca gtagcagatg ctcgtgtagg aggcatgtta tccggaaaag ctgggatcat
     2641 caatgtccac ttaggcggag gaaaaagaaa gctagagcta ttgacacgaa tcgtagaaga
     2701 aacagaaatt ccgatcacac aatttttgcc tactcatgca aatcgcacac cagaattatt
     2761 tgaagcttgc gtggcatttg ccaaacgtgg tggcacgatt gacttcactg ctagcgaaga
     2821 tcctgatttt tgggagaaga cagatggtga agttcgcttc agcaaagcac tgaaacgatt
     2881 gatcgaggaa gatgtttctt tagataattt cacgatgacc tcagatggac aaggaagctt
     2941 gccttatttt gatgaaaaca atcatttcct agggttagga gtaggaagtg ccaaggcatt
     3001 gctagtaggg atcaaagaag ctgtgcaaaa agaaagtatc cctttggaaa tcgccttacg
     3061 agctataacc agtaatccag cacgtatatt gaaattggat aaaaaaggaa aaatcgaaat
     3121 tggtgctgat gcagatctgt gtatcttgga taaagaaacg cttgatattg atacagttat
     3181 cgctaaagga gaaataatgg tccaagaaaa agaagtcaaa gtatggggga ctttcgaaaa
     3241 aagtttttaa tcaacagctc taactatctt ttttagaaaa cgagcgttct gtttgtctgt
     3301 atctgttgtt gatgttaccc tgaaggtaga tcaataaaca aactaagagg aggaagcaca
     3361 atggcagatt taaaaggacg ttctaaagat atgaaagata aagtagtcgg tgaaggaaaa
     3421 gaagcatacg gcaaagcaac agacgacaag agcaaagaaa cagaagggaa agcacaatca
     3481 ctaggtgctg acatcaaaga aaaagctcga aatttaggcg acgaagtcaa agacaagttt
     3541 tctcgaaaag acgaaaaaaa ataaaaacca cttattacta attgaatcaa gcaaataaaa
     3601 aatccgaacc atatctgcga gacagatatg gttcggattt tttatttgat cattatcctg
     3661 ttttttaaac ttcgattttt tgaaattttt tattttttag ttcaaagact tcatcacagc
     3721 tttttgctac ttcaatctca tgagtcacga tgataataca gcgattttgt tcatgggcga
     3781 tttcttggaa aagtttgacg atatcttgtg aagttgtttc atctagattt ccagtaggct
     3841 catccgctac gattagttga tgttcacagc agatagcacg aacgatagcg actcgctgct
     3901 gctgacctcc cgaaagtttg gtcacctgtt tttttgctaa tatttcatca attcctacac
     3961 gagccagtcg atctaaagca acttgtcgat aattctcttg cttggtctta gtgatttcca
     4021 ttgctgctat gacgttgtct agagccgata aataaggaag caagttgtaa gcttggaaaa
     4081 taatcgagac atcattttta cgataacttc ttaacccgat tttgttcagt gaacgatctt
     4141 gatagtatac ctttccttct tttggtgcat ctagtccaga aataagcgaa agaaaagtcg
     4201 tttttccaga accacttgct cctaaaatag cgtacatttt tccagcttcg aaattcagtt
     4261 ctacatcttt ataaagcgca tcttgcggat cttgatacca aaatcccaat tgttctgtcc
     4321 gtaacatcaa attcctcctt tagtttaata ggatttttct tgggtttaat cttaatatcc
     4381 ctatagaagc taaaaagaca gaaaacagac tgattccaaa tgctactccg gctaatagtc
     4441 ccaattgtgc aggtttgaca ttgatttcca attcgctgac ttgatcagaa acagcaaatg
     4501 gattattcgt ggttccttgt tgaccatcgc gttgaccgcc ttgttcttgt ctgttacctg
     4561 gcatttgtcc accgccagga gcttgctggt tttgtgtcgt ggtttctgtc tgttggtcaa
     4621 gtagctgctg tcccacggca ttcgcgacga cgtttccgct aagcgaagca actcctaatg
     4681 caagaatcat acaaatagcc acttcggtaa acaattggag aatgatttta cttctggatt
     4741 ctcctaaaga tagcaagacg cctaactcat gccgacgctc ccgaatggta atcataatga
     4801 tcagagtaag tatgataata ccggctacag ccactagtaa aacaacattt ttagaaaaac
     4861 ttgccacgtt gtttaaaggt tccaacattg actgatacat ggaatcattt gattgtaagc
     4921 taaatgtatc tgtgtcgatc agcttctcag cttctttgag aaactcatcc attttatctg
     4981 gatcagatag agtgtaaata gcagaatcta tcgtgttatc gtcagatgtc ccatttagtt
     5041 catttgcaaa agtgtaagaa gtgtaaagtg tattcgacgg attcatgaag ttgaatttca
     5101 tacccatact gccgcttgtt tcgctggatt cataaattcc tttgatcgtc atttcataag
     5161 tggtatcttc atcttcagta ctcgtgattg tgaaagtatc tccgactgcc aggtcatttg
     5221 tttctgctag agtagaatca atgactacat tgtttgtgcc tttgtcgctg tcatctattc
     5281 cttcaccatc cgtgattttt gctgttccat cagaaaaatt actattttgt gcagttgctg
     5341 acacaccact gatttgaaag tctgcttgag tcatttgcgg attcccgccc attcctccag
     5401 caggttcacc gcctgcatca gtagtttctg aactgtcttc acttgaatca gaactagtga
     5461 tggcagttat tccatctttt gccatagctg aggcggaact ttcaaaggaa taactcttta
     5521 cattatctaa ttcagctatt ttttccgcgt ccgttaagga aactggtgta agagaaaaac
     5581 tgcctggatc tggacgttct tcagtggtag aaccgcttga agaagtttgt tcctgttttt
     5641 taaatgaagc ttctcgattg gcacttaaag tcactgttgc tccaacttct ttttgtgctt
     5701 gattcgctgc ttcttctgca gcgcttcgaa tcgtgaggcc agctagcaca aaaataagaa
     5761 tggctgtgaa cacggcaaac aataacaatg aacgtcccca ttttactttt gtacttttcc
     5821 atgcacgttt cataaaattc atattttgca tccctcctag tagatgcatt tgataataga
     5881 caacctatct taaagctagc ttaaatctta tgctgtttgc acaccatcaa catgaggttc
     5941 tgtatatata aaaagtcact atagttattg caaaaaatgt ggtaaaatga aaaaagtatg
     6001 tttcaaaccg acgaagaaga aatgaaagag gaatagcaaa agaaatccta gatgctacag
     6061 agggattttt acgtgcatag ttgttctaac aattgctgat actagcgatt tagtttattt
     6121 gttgtataat aatggggaac agctgaatac ttatataagt attctggcta tagcgtttcg
     6181 caaaagatcc attttgttaa tgaagtatat ccaaaataaa atgcccatcc aactgtcaga
     6241 acgcggatgt gctggtaatt ttcctgctga ttctccataa gttgagaaat agtgattttg
     6301 catatgcttc attggaatgg ctcatatatt tagataaaga agaaaatcga catctttaaa
     6361 cttggatgtc ataagaagag gtgaagtttg tgagtaccat taaaattatc ccattaggcg
     6421 gcgttcgtga aaatggaaaa aacatgtatg tcgcagaagt gggcgaggat atttttattt
     6481 tagattgtgg gctaaaatat cctgaaaatg agttgttggg gatcgacgtc gtaatccctg
     6541 attttacgta tttagaagaa aacattgatc gtgtagctgg tgtcttttta acacatggac
     6601 atgcagatgc gatcggggca cttccttact tgttgtcaaa aatttctgta ccagtattcg
     6661 ggacagagtt gacgatcgag ttagcaaaat tgaatgtcag tcgtaatgaa gcagccaaag
     6721 gatttaagga tttccatgtg gtagatgagc atacagaaat agactttggt gaaacagtag
     6781 tgagcttttt ccgtaccact catacgatac cagattcagt ggggatcaat ctgaaaacaa
     6841 aagaaggtag tatcgtttat acgggagatt ttaaatttga ccaaacggct atcccaatgt
     6901 atcaaacaga ttatgcacga ttggcggaaa tcggcaaaga aggagtctta gccttactaa
     6961 gtgaatcggc aaatgcagaa aatcctcagc ctgtagcttc tgaagcccaa atagcagatg
     7021 aagtatttga tacgatcaaa tattgggaag ggcgtatcat cgtcgcttgt gtagcaagta
     7081 atttgcaacg agtgcaacaa gtattggatg cagcagaaaa agcacaacga aaagttgtct
     7141 tgactggtca agatttcgaa cgaattattc gcacagcgat gcgtttagaa aaacttcaat
     7201 taccaagtga agatctgctt gttacgttga aagacatgaa aaactatgat ccagaagaac
     7261 tgatcatttt agaaactggc cgaatgggtg aacccatcaa gtctctgcaa aaaatggcaa
     7321 acggtacaca ccgtaatgtc aagattgaag aaggagattt ggtttatatc actactacgc
     7381 caagcatcgc gatggaaacc atcgtggcga aaacggaaga tatcatttat cgagctggtg
     7441 gaacagtgaa gttgatctct gagaatatgc gcgtttctgg ccatgccaat cctaatgact
     7501 tgcaattaat gatcaatttg atgaaaccaa catattttgt gccagttcaa ggagagtatc
     7561 gtgaattagc tgctcatgca gatttggctc atgcagtagg gatgccatac aaaaatatct
     7621 atattactgg acgcggagac gtgctggaat ataagaacaa gcgtatgagt gttgcaggaa
     7681 ctacaagtgc agaaaatgta atgattgatg gattaggcat aggtgatatc ggaaacattg
     7741 ttttacgtga tcgaaaaatc ttgtcagaag acgggatttt cgtagcggtt gtcactatca
     7801 gtcggcgaga aaagaaaatc atttcaaaac ctcaaattac ttcacgggga ttcgtttacg
     7861 taaaagctag ccgtgatctg atgaaagaaa gcagcaatat gattgaaaca atcgttgaga
     7921 aacatttaga aagcaatgat tttgagtgga gcaaattaaa gcaagacatc cgagatcaat
     7981 taggccgtta cttatttgaa caaacaaaac gccgcccagt gatcttgcca gtcattatgg
     8041 aagctactca acgtcgaaat aaaaaataaa caaaaagagt aagatgaacc tctctataga
     8101 gtgctcgtct tgctcttttt ttgcgaaaaa caaataattt ttgaatacag catccctcgc
     8161 tttataataa acgatagaat aaagggagtg aatcaatgat gagtcaaaca aaagtcgaac
     8221 gttatttaga agaacaaaag atcagctatc aaccacttga ttttagtcat gtggtagaag
     8281 aatctttcac agaagaacta caaaaaagag ggattgatcc agctttgata tgtaaaactc
     8341 tcgtagtgaa aggaaacaaa actggggtga tcatcgcatt gattcctttg aacgatcact
     8401 tagattacaa aaaaacgaga caagtgacga aagatcgtaa agttggtttt cctggaatgg
     8461 attttgtatt aatgcataca ggatatcccc acggagcaaa cacccctatt ggaataaaac
     8521 tcgcacatcc tgattattgt tttttagcag acagttcttt aaaagaaaaa gaagaagtaa
     8581 ttgtgtcaag cggtgaaatc gggagaagcc ttcggataaa agtagccgat ttagaaaaga
     8641 tcgtccatcc tatttacgct gatttaatta gttaagggac gagaagagaa gctaagcaga
     8701 gcagagacct caaacttgag attatatctg cttggtactt ggtgcatcct gtattatctg
     8761 ttacaattgc tttgtacata ctgtactatc aatccaatga aaatatattt agcagctata
     8821 ttttttcatt gatgtgcaag tgatgaaatc gtttttaata aaaaagatac agagaaaacg
     8881 cgaggtgtaa aatatgaaaa ttacgtatca tggacattcc tgtttaacaa ttgaaatgga
     8941 aaatggccaa cgcgtgatga ttgacccgtt tattacagga aatccattga cagacttaac
     9001 tgcagaagaa gtaaagacag attggttgct gattactcat ggtcatagtg accacatagg
     9061 agatatgatt cctattgcaa aagaaaatca agccacaatt atcagtattg ttgagattgc
     9121 tgattatgcg aacagcagag gagtagattc attcggtatg aatatcggtg gaaaacatgc
     9181 gtttccattc ggcgtggtaa aattcgtaca tgcacagcat agttcaagct atgaagtaga
     9241 cggtatcgtt cagtatatgg gcgagcctag cggaattatt attcaagcag aaggaaaaac
     9301 gatttatcat gcaggggata ctgcttattt cagtgatcta ggattgcttg cagaagaatt
     9361 cgatattgat gtcgctttct tgccgattgg cgataactat acgatggggc cagaagatgc
     9421 agcaaaggct gcgcaaacaa ttcgtgccaa gaaagtagtt ccaatccatt acaatacttt
     9481 ccctgtcatt gaacaagatc ccgaatcttt tgtagagtta ctggaagata gtgaaggaaa
     9541 aataatgagc gtctcggaaa cgctggaagt gtaggcagaa aatggcgaca aaacatgatt
     9601 tgattttaga atatattgaa agtttacctg ttggcaatcg aatttctgta agaagcatcg
     9661 caaaagaatt gaatgtcagt gaaggaacag cgtaccgtgc aatcaaagat gcagaaaatg
     9721 tcggactagt ttcaacaatt caacgagtag ggaccatccg tatcgaacgc aaattaaaaa
     9781 aacatattga acgtttgaca tttggcgagg tcgtcaggat cattgaaggg gacgttttag
     9841 gcggggcggc aggattagac aaggtcttga ataaattcgt catcggcgcg atgactaaac
     9901 atgccatgga acgctatatt acgccaggat cactaatgat cgtcggcaac cgggcggaag
     9961 tccaaaaatt agcattggaa gatggcgccg cagtattgat cacaggagga ttcaatacga
    10021 ctgctgagat tgcaaagcta gctgatgatc ttgaaatgcc gatcttacgc acctcttatg
    10081 atacatttac agtcgcaacc atgatcaacc gagcgttaag cgaccaattg atcaaaaaag
    10141 atatcatgct tgtcagtgat atctatatgc cagctgaaaa gactcattat ttgcagcaga
    10201 cagatacgat ttctgattat cagcgtttgt cagaagaaac gcagcattcg cgttttccag
    10261 tcgtcaaccg ccatcatcgt ttgatgggga ttgttacggc aaaagatgtt ttagggaaat
    10321 cgccaaacca gatcatcgat cgggtgatga cgaaagaacc aatcagtgta aagaaaacaa
    10381 tgagtattgc ctctgttagc catcaaatga tctgggatgg gctagaagtc atgccggtcg
    10441 tttccgatga cttgacattg gaaggcttga ttacccggca agatgtaatg aaagcgatgc
    10501 agcttgtcca gcgacaaccg cagattgcag atacgatttc ggatcagata tctggaaata
    10561 tccatacgat tgatactgac cgtgaaggaa atcgtttgga aaaccctaag ttcaaattcg
    10621 tagtagcacc acaaatggtc aatggcgttg gaaccatttc ttttggcgta cttagcgaaa
    10681 tcatttctga tgtcgcacaa aaaacaatgg tgatgaacca aaaacgaaat atcttgattg
    10741 aacaagtgaa cctacattat ttgcggctga tccaattgga aagcgaatta gacattcgtc
    10801 cacgtgtatt ggaaatcggc agacgttccg ctaaactgga tatcgaagtg tttattgaaa
    10861 acaccatcgt ttccaaagca atcgttgttt gtcaagtaat ggaacgttct tagaaagcag
    10921 gagtgaaatg acagtacagc aagaaatagt agatacaata aaaaaatacg ataaaatcat
    10981 tatccacagg catttgcgcc ctgatcctga tgcattagga tcacaagtag gattagccga
    11041 actattacgg gaaagtttcc cagaaaaaga aatacgtcaa gtaggtgaaa cgattgaagg
    11101 gcttcgcttt ttagcagaaa tgcaagaagt tgaagatgtc ttctaccaag gagcgctagt
    11161 catcgtgact gatacagcta attcacccag aatcagtgat caaagatatc agttggcaga
    11221 aaaactgatc aaaatcgacc atcatcccaa tgatgaccct tatggagatc ttgtgtgggt
    11281 aaatacaaaa gcaagtagct gtagtgagat gattgcagaa tttgctttga tgtttcctga
    11341 atctttaaag atgaacgtta aggctgcacg gttactttat gcaggaatcg tcggagatac
    11401 agggcgcttt ttgtatccct ccacgacgtc ccgtacatta gagattgctt cgatattgag
    11461 aaattatcca tttgatgcaa gtgctttgaa cagagagatt gaacaaatgc cgatgaaagt
    11521 cgctaaactt tccggttatc tttatcaaaa tatccaggtg gatgaaaatg gtgcaggaag
    11581 aataatcctt ccgcaagaaa ttcttgataa atatggaatc gatgattcag agacagcttc
    11641 gatcgtttca cttcctggag tgattgacga agtgctggct tgggggattt ttgttcagca
    11701 gccttcaggg tattaccgcg tacgtttacg ttctaagggg ccgatcatca atgaattggc
    11761 taaacgccat catggaggag gccatccatt agcaagtggt gcgaatgcaa aagatttagc
    11821 agaagtagag gaaatctata aagagatcca agatatatgc caatcgtata agagcagaaa
    11881 aacagaatga tacttcgttc gaaaacgtgc cttttgtagt aaaaatcaca aatgtatctt
    11941 ttcaagatct tctttttccg ttacaatagt gctcactaaa gattttaaag gagaaagaag
    12001 atgacattac caaattgccc agaatgtggc tcagagtaca catatgaaga tcgaggcttg
    12061 ttgatttgcc cagaatgcgg gaacgaatgg aacaaagaag aacaagaaac tgtcatagaa
    12121 gacggattag tggtattaga tgcaaatgga aaccgtctgt tagagggcga tagcgtgaca
    12181 gtgattaaag acttgaaagt aaaaggtgcg tcaggtgcca ttaaacaagg aacaaaagtc
    12241 aagaacatcc gtttagtaga aggcgatcat aatatcgatt gcaaggtaga aggatttgga
    12301 ccgatgaaac tgaaatcgga atttgtgaaa aagaattaaa gaatattcaa ggtcttttca
    12361 cattcttttc ctactactaa tgataaaata aagtaaacga aaaggagtga aacgaaaatg
    12421 tatggttatg gaatgtatgg ttttttagat ccgacatttc ttttggtcat tcttggatta
    12481 gttatttccg gaattgcgtc agcgtacgtc aatagtactt tccgaaaata cgatcaagtg
    12541 agaagcaaaa acaatgtgac tggtacacaa gcagcacaat atatattaca aagtcaacag
    12601 ataaataatg tcggtgttca acaaattgcc ggagatctca cagataacta caattcaggt
    12661 aataagatgt tgagtttgtc tcaagcaact gcacagtcta cttcagttgc tgcgataggt
    12721 gtagcagctc atgaatgcgg acatgcggta caagatgctt cgggttatgt cccattgaag
    12781 attcgtgcag cacttgttcc agcagctaac tttggttcga tgatttcgtt ccccttgatt
    12841 atggtaggtg tattgcttag ctggaactct acgttaatca atatcgggat tttcgctttt
    12901 tctttagcat tacttttcca attggtgaca ttaccagtcg aatttaacgc gtctcggcga
    12961 gcaatccaaa ttttgagtga aggcggacta ctgacggaag aagaagtacc gatggcaaga
    13021 cacgtattat ttgcagcagc actgacttat gttgcagcag cgctttcgac attcctgcag
    13081 ctgttgcgct tgatcttatt gttcggcggc aaccgaagag attaaaataa agaagttgtg
    13141 aaatcagcgc tttgatctga gctaataccg agagttagct gattgaatac catttatttg
    13201 gaataagggc tctttgtcaa taaggactga tgggttgtac aaaatcaaat ctgggtcaga
    13261 atgaacctca ttctggctca g
//
LOCUS       DACDTH010000077        13274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDTH010000077 DACDTH010000000
VERSION     DACDTH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.078"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1227)
                     /locus_tag="IWK54_002397"
     CDS             complement(<1..1227)
                     /locus_tag="IWK54_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9594491.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
     gene            complement(1224..3413)
                     /locus_tag="IWK54_002398"
     CDS             complement(1224..3413)
                     /locus_tag="IWK54_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9594492.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            complement(3547..4737)
                     /locus_tag="IWK54_002399"
     CDS             complement(3547..4737)
                     /locus_tag="IWK54_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP9594493.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(5609..7240)
                     /locus_tag="IWK54_002400"
     CDS             complement(5609..7240)
                     /locus_tag="IWK54_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9594494.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(7254..8927)
                     /locus_tag="IWK54_002401"
     CDS             complement(7254..8927)
                     /locus_tag="IWK54_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9594495.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(8940..9257)
                     /locus_tag="IWK54_002402"
     CDS             complement(8940..9257)
                     /locus_tag="IWK54_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9594496.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(9282..10682)
                     /gene="lacG"
                     /locus_tag="IWK54_002403"
     CDS             complement(9282..10682)
                     /gene="lacG"
                     /locus_tag="IWK54_002403"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9594497.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10919..11704
                     /locus_tag="IWK54_002404"
     CDS             10919..11704
                     /locus_tag="IWK54_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9594498.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            11707..12399
                     /locus_tag="IWK54_002405"
     CDS             11707..12399
                     /locus_tag="IWK54_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9594499.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(12673..13044)
                     /locus_tag="IWK54_002406"
     CDS             complement(12673..13044)
                     /locus_tag="IWK54_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9594500.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         4082 a         2713 c         2180 g         4299 t
ORIGIN      
        1 taaaatatcg tttgcaatta gctttcttct tcgaaccttt tcacctgatt cagtaaagta
       61 caaaattgct aacttgaaaa ataaactatg attatttacc ttaattggtg atatatttat
      121 agcatcaaaa ttgcaatacg gtaattcttt ctttaaatac tcacccagtg aaaacagagg
      181 atagtgttga ggagcaagta cacaaatatc tttttcatga atgccgcttt caatcttttc
      241 ctttattata ttttttatta tgttgggtaa atcctcctta gtaactttgc aatcataact
      301 tataaaacca cggtcgtttt tattattccc tctgctttca atctcgtaag gacaactctg
      361 aaaattagag tagaagtcta ctaatctctg attagacctg taacagccat gtagcgtctt
      421 tgattgaaaa gtgtgactag ttagcatttc tagttcttca actgatttag ctataccatc
      481 taatgatcca taaattgctt ggtcaatatc tccaacaaac attgactgaa tttgagggtt
      541 gttttgaagt aatttagcta ttacattgta ttgtaattct ctagtatctt gaaattcatc
      601 cacataaatc gcccttatat tactagcaat attacgtgca acgttactgt ttacagttaa
      661 aattttatac gctactagta aaatcatatc aaaatcaatt tctttatttt tttccaacat
      721 agtgtgatac atttcagaaa cttgagtata ctgaacttca ttaattttaa aatcatgaga
      781 aagtgacaaa tcaattttgt tcagttcata cgttttgaga tttaaattca gcatattatt
      841 aatttggctg acgtaagctc tttgagtata atcatcaata attgtaaatc cttttgatac
      901 tcttgagaga ttcattttaa aaggataaat aataaattca agacaaaact gatgtattgt
      961 ccccacccaa atattaatat catcatctat actaagtaaa cttaggcgtt ccttaatttc
     1021 ttcagcagct ctattagtgt atgtgatagc aattattctt tttagtgatc catcgtgttt
     1081 aattgcttgg taagcaattt tccttgtaag tacccttgtt ttcccactcc caggacatgc
     1141 atttacataa aggttatctt cactttccac aacatctttt tgttctaaat ttaaatcatt
     1201 tacgattttt tcgaagatat ttgtcatttt gaaacctcag cataaagtat ttttgtgtaa
     1261 ttttctatct gattgattag atacacaaca tcctcatctt taccaaagta ctctaataaa
     1321 gaaattattg ctatattcaa atcagctgta tcacttgttt ttaaactagc tgataaagct
     1381 gacaattctg tagtttcctc gtacaagtcg attgtatgga gtagaatttt tttataaatt
     1441 gagagatcta ctatttcctg acatgcaaaa gcaatagcat taagaatata cttaggaatt
     1501 ttgattaaaa agtcaatttt ttcacctaac tcaattgcaa accacccctt accaacatga
     1561 ttggctagtt ttaacatcgc taaatttctt tctaaatatt gttcactttg tatttcttct
     1621 ttataagcag ttattgtttt tttatctttg aatgcgtcat ctataattgg actgacataa
     1681 gttagaatat tttctttatt cacatctaaa aactcaattt caaatgttgt attagcataa
     1741 aaacaattaa cccaattgtt ttcattaaaa agggaggata atttttcttt tcttgcttga
     1801 cctttctctt gtgcagtgct tttatacaat cgatgttctg atgggattgc ctgttcatct
     1861 aaatcagtta caatagcaca ctttctctgt attctatcat cgtcaaacaa gctagcgata
     1921 tattcaaacg aagtactacc aacattaatc aaacctattc caagttcgtc taagtttata
     1981 ccaaataatt tttttactat ttgaggaata agtatttctt cagcatctcc ttcaactaag
     2041 ataacacctt tagaaaaaag caatccattt cttttaacat ctaaatatct ctctactgcg
     2101 tttattagat ttagattgtt cttttttaac ttttctttag caaatgagtt cagattattt
     2161 gctggattca taacgataga tttgccatta aacgatttta atacattcat ccgagatatt
     2221 tcagacactt cagctaaatg tacagaatgt gtggtcatga gaatctgagt atagtttttt
     2281 tgaagattta atccttcgaa cagagttttt tgaatatgat gatgaatatg agcttctggt
     2341 tcttcaatca acataatatt aagtagttct cttgaacgac acgcttcaaa ttcgactatt
     2401 ttcaaagcta aataaatcat atttagatgt cctaacccta gtaagtctaa gtcgatattt
     2461 tgttctggct tcattgaaag aaattttgct aaagaaccca tatcatctga catagttgag
     2521 gaaagaagaa gattaggaga atacactgaa ccaattatgc tatttagttg ccgattaata
     2581 ttttcgccaa ttttcctaat ttcaggaaca ctggtaatag tctcatttaa ttgctgtata
     2641 attgttttta atgactcaac attatctgat gatatttttg attcaattgt ttccatgatt
     2701 cttttgactg ggtttcgatt gtttttcatt tctctaagaa catctcgaag agcgtcaaca
     2761 taaacaaccg aaatatgctt gaaaatatct gacatgtcaa ttcttgagcc caacagagct
     2821 tcatcgtctt cttcaggatt actagctata ttgtcattga gtcttcctac caatttatca
     2881 taattttcat tgatacagaa atttagattt gatttagatg tgtagtaaaa ttcatagtcg
     2941 gatagacgaa tagaatttct aaattcgtta aattgatgag agtctaattc agtggaaata
     3001 tcaaataatt gctttctaat agctttgtta ggtctaataa atagagaaat actacctaca
     3061 cctttatctg catttgaaat caattcttct aagattgttt gactttcaga atttaaagaa
     3121 atggaagcac aaatctcatt gtcaaattct tcttcgctga ttccttcaaa tgtcgcagag
     3181 ataataatcc aatgcccttg ccacccattt tgaattccat gaaaaaaatc tgattctttt
     3241 agtgttttac tactatagta ataactatca tctaataaga ttctcaatgc agtaagagca
     3301 ttggttttcc ctgaatcatt ctcacctata atagtatttg tctcattatc gaatcgaaag
     3361 atttcggatt cataatttct aaaatttcga atttgaagag tagatataaa cataagcaga
     3421 tctcctaact atttaattaa tatgattata tagtaagttt atcataaatt atgttagtaa
     3481 aagttctcat tataagggag cgtcaataat tttgtgtaaa tgattcttcc ctactgcaga
     3541 ttgtttctaa ttctcaatca gttggcttag catttgttcg agctcgcctt cggcctgttg
     3601 gaaaccttta tgactgcgac ctaggaattt ttggttgtaa gtatcaaaag acgagactaa
     3661 gaaacgttcc atcgactctt cattttggaa ctgttccttg cggtggctgt atttcttgat
     3721 ttgtttatta aaggattcaa tcaagttcgt agaataaata ctcttgcgta tggccaaggg
     3781 gaaatcatag aaagtgagca agtgatcgtt cgaaagaatc gattcaacca cttttggata
     3841 gctggttttc catttttccg caaaagtacc acgtgcttcc aacgccacct ctttagatga
     3901 ggcttgatac accattttaa aatcatcaca gacttcctta cgatcatcga cacgcacttt
     3961 atgactgata ttacgggaaa cgtgtacaca acaatgttga aaacgagctt tgggatagaa
     4021 ccgactaatc gctcctacca tcccctttaa gccatccgtg atgaacagga ggacattttt
     4081 caaaccgcgc tcttgaaggt ccaataaaat ttcctcccaa atcgtgatgg attcagtcgg
     4141 tgcaatcgca tagctcaata cttcctttga tccgtccggg cgaatgccaa ctgcgatatg
     4201 aatagcttcc ttggcgacgg ttttccgctt taacggaata tacgttgcgt ccatataaat
     4261 agcagcataa cggtcatgaa gctcccgccc attaaacgcc gttacctctt ctgtaaatga
     4321 ttttgttata ttggacatgg tttggggcgt gtagtaatgc ccatacattt tctcaatcaa
     4381 gtctgcgatt tccgacatgg taataccttt tcggaagagg tgaatgacgg tctcctctaa
     4441 cgtgtcattc gtccgtctat aagcaggaac agtctgttgc ttgaactcac cattgcggtc
     4501 gcgcggaatc tggagatgaa gttccccata ctcggtcttg accgtacggt cataggagcc
     4561 gttacgagaa ttacccgtgt taaaaccaat gcgatcatac ttttcgtaat ctaggaaagc
     4621 cgttaactcc gttttgagaa gcgtattgac tgctttctcc aaatggacac ggaataattc
     4681 attcaaatcg cctttgttcg ctagagtctt tagaatttct gtagtaaaat cgttcatagg
     4741 gaagtcctct cttctgtgaa tgtgttgtgg taactctatt ctacagaagg gacttccttt
     4801 tttctattta cacaaaatat tttacactct ccattataac aattgggtta aaaagaaaat
     4861 agatagtatt ctacctcata tttatttgaa agtaatatat aaaagacgtt caaattcgaa
     4921 cgcctctcgt taaccaaata ttggattcag aatctagttt tcatatataa acagagtatg
     4981 attaggtgca aaaattgcac ttttattgca cttttatttt tatttatagt gttttactgt
     5041 tctgtatatt gttttaactc ccttgatagt atcgcattct gttttataca ctttacgttg
     5101 aaaacagagt gggaataaag agccaacata atatggaggg ggaatcccta taaatcaaca
     5161 ttcttcaata gctaaaatgc ttgattttca ttaaaaataa gatggttccc taccaaaaga
     5221 gactataata taagttaatt tgtttcattc ttatcataaa aagaagttct cgaaaaacga
     5281 actgttttcc gagaactttt ttctatccat ctaagtgctc aaatcatatt cacgacggaa
     5341 ttctaaggtt accctttctt aaacaaactt tatgttatct gcacaacttt ttgcaatttt
     5401 ttcattgctt cttacgaaat cgttgaactc tctttagcat aatacaaagt gtagatgaag
     5461 tctggtgctt tactcgtttt ggtaaaaatg actggaaatc gtttctggtt atttttggca
     5521 aaggcgaggt gcaggttcga ttcctgcctt ctacgctaac gatgtggctg aagggaacct
     5581 ccgatcttga atcgaaacga tccgcagtct attgtttcac caatgaaaca aaatcaacgt
     5641 tcgtccaagc atttcctgaa gccttgctat aaaccccaat ttcacaaaca cctgttgtaa
     5701 ttggaatgtc tttgattgta atttttgtcc attcacttgt tttaacaacc gcagtattta
     5761 gttccgctcc accataattt ttgatatata actgattagt atcaaactct cccccagaca
     5821 ttacataggc actcaatgtg taagttcctt tgcttatgcc actaacattt tgataagctg
     5881 atgcctcgta tgcttgatcc tgccaaaaag ttagtttgta gtccccaaaa aaagtgcccg
     5941 cttccgtttt gatcgtaccc ttagcggttc cttgttgaaa ccaggtttgc caattggaag
     6001 gactggtcgt tggtccatcg ctttcgaaac ttgcattttt aactagattt atttgcgccc
     6061 ctgcttgttt aaacgcacgc aagcttggca aggcgtttcc agcaaaatca aacatcgctt
     6121 ggttgtccca ttcgtttccc atagtggatt gaacactgag ataattcata cctgcttgtg
     6181 ttgcccaact cacatttccg ttgtaccaaa gaggttccca atagaagaag cccgccccgc
     6241 gattattcgg tactgcctga atccgatcgg caagatccag caaatactct gcttgtcctg
     6301 tcggtgttgg ttcatatccc cccgcagctg cttcggcttc accaaaagca tttggcatag
     6361 tatcggcatt cgctgttgta tgcccataag ctgtttcaac tacgatcact tctttaccat
     6421 accgttggct gatcgcattc atattggttt gaagttcatt cattgaaccg tgccaatacg
     6481 gataatacga cactccaatt aaatcaaatg acacacctcg actcgtgatt tcatcaaacc
     6541 accaagtaaa tgctcctgta tcgccaccat cagctaagtg gagcattact ggagttgttt
     6601 taggttgagt gtctcgaaca gcttggatac cagacttcaa aaaagcagcc aatcgatcaa
     6661 attctccccc gccttctccc caagacttgc cataaggcca gagcattccg ctattcagtt
     6721 cattgccgat ctgaaccata tcaggataaa cgtccagctg cttcatttcc gacagaacgt
     6781 tctttgtata gtcgtacaat gccgtattca tttcttcaaa ggtcagacct tgccatgctt
     6841 taggaagatt ctgtttccca ggatcaaccc aaaaatcact gtagtgaaaa tccagcagca
     6901 ccttaagtcc cagatttttt gctcgttttg caaggtcaat cgtcgtttcc agatcatttg
     6961 tccctgctcc ataactttct ccattactat tataaggatc ctgccacaaa cgcagtcgaa
     7021 catagttgac accattattt tttaaaatct ctaaggcatc ttttgaaata tcgtcttcat
     7081 aatattttgc accagatttt tccatatctg acaggatcga aatatctgct ccgcgaataa
     7141 attcgctggc agatgcttca acagtttttg aaacagcccc caaagcccct aacacagctg
     7201 caaataaaac agctttacgc aaccaaattc ccattttcat tatggctcct cctttattga
     7261 aattggttca gaataaactg aacagctttt tcaggatctc tcgttaatcc gatatattct
     7321 gctccttttg tcgcaaccag cttgattcct aagcgatcgg tgtcttcttt gatatcatca
     7381 tagtaagaat tgacttgcgg tgctaagacc accatatcaa aatcctgcag catttcgtaa
     7441 tgagaaccat aagcacccgc cattgcactg atcggtgctt tcgattcaga tgcacctttt
     7501 tttaaagcat tagctaacat cgcacttgtc cctgcgccag cacaaagtac caacacattg
     7561 atttctttat ttgcatcgct gataacttga ctcgctgcca caggtacttc ctcacgtaca
     7621 tcggcagaag caccttcctc ggttgcgatc acctctgctt tttgtgcttc tttctctact
     7681 aagacagcat cgtatgcttt gcaaaatggt agatagatca ggaagtcaac gacgattaga
     7741 accgctgcta gaacaaaagc taagataccc atgcccgttc ctagaaccaa accgatcggt
     7801 gctggagttg cccaaggcaa aacgtacata aagctgttca tgcccaatac atcgacaaaa
     7861 aacttgaaca ggcaaacgtt tacaacaggt gttaacaaaa atggaataaa aaaataaggg
     7921 ttcaaaatca tgggtgccgc aaatagcaat ggttcgttga cagcgaaact tactggaata
     7981 aaggaagctt tcccaactgc cttcaactgc ttagacttag caaagagtag gaagataaaa
     8041 ggaacaacca atgttgcccc agttcctccc atagttccta caaaattccc taaacccacc
     8101 gtcagtactt tagaagcttg ttcaccggaa ttgaacaatt gcagattggt ttccacattg
     8161 gcatagataa ttgccgcaat tgccggctcc acgatcgacg gtccatgaac accgacaaac
     8221 caaaacagcg ccatcgctcc ccagataata gcgattccta agtacccgtc agctgctgta
     8281 aatagcggct gcagcaccgt aatgattgct tctgcaaatg agatttggaa gatttgtcga
     8341 atgcctaaat caattaaaac ggccgcaaat actgaaaaag agaaaggaaa aatatctcgg
     8401 aaggtttgcg aaatcgttcc aggaacttct ttaggcattt taatggtaat atctttcagc
     8461 acacaaaact tatataaatt aactgttaaa aatgcagcga caaaggaagc cagtagtccc
     8521 tttgtcccca tatattccgt tgaaaatcca ccttcaatcg gagagacgct taacaacagg
     8581 aaacttacca cagaggccag catcacagag gtggtgttga ttactttgtt tttcggtaag
     8641 cgacgattca tcgaatcagc caagcagcga gcagtggttg ctgaaacgag taaccctaca
     8701 acacccatcg aataattata gactttccat agccatgtgg agacagattc aggccattgg
     8761 taaccaaaaa tctcaggaac cgcagcaatc aaaatgaaaa tgcttgaaaa taaaatagct
     8821 ggcatcgctg ttaaaaatcc gtcacgaacg gctcctaaat aactattacg tgagattttt
     8881 tcaaaaaatg gtttcccttt ttcgatttgt ttaatgattc catccatagc gttcttctcc
     8941 tatccttgat tctgtctgta cagtacaata aagtcttgaa tcagatctct cagcaataaa
     9001 gtggtcatta aatgatcctg cccatggata aaaatgaatc ccatctccat ctctttccca
     9061 gatgcttctt ccgctaaaat ttttgtttgc gaattatgtg caagggttaa attttcgtcg
     9121 gcttctttca acgccgaatc aacatgttca aagtttcctt gtcttacttc tttcaataat
     9181 ttcaataatg tactgcgagc gtcgcctgaa tatgcaacga tctcaaaacc aagcatttgt
     9241 aattcctctt tattcatctt ttcttcctcc attatcactt attaaataat cgttttggtt
     9301 tcactgacca acttgtacca gtaagctgat tcttttgggt aacgcttctg ggtttcaaaa
     9361 tcaacataaa acaagccata ccttttgtta tacccatttg tccatgagaa aagatccata
     9421 agcgaccaca aaaaataccc cttcacattt accccggctg ttatcgcctt gcttaaggat
     9481 tccaaataga ctctcaaata gtcgattctc ggctgatcca taataatgcc atcttcaaac
     9541 tgatctttat accccatccc gttctcagtg atatagattt tgttgtaatg tgggtaatcg
     9601 cttttaattc gcagcaacaa gtcatataga ccttctggat aaatcagcca gtcccaatca
     9661 gttcgaggaa ttccttcttt ataaatacgt tctccgattc ccttcacttt ataaacagag
     9721 gtcccttttt cacccgtacc gttatgatgg attgcatttt ctccatcgta agctttgaca
     9781 aaatggcatt ggtagtgatt gatccctaag tagtcattac gcgtggatgc ttttttcaat
     9841 tcaacgaaat cttcttcagg aaaatgatag ctggcctgat ttgcttcaca tatctcatca
     9901 agagcagtca aggtttcagt tgaataatag cctaaatagg tagcatctaa taagaaacgg
     9961 atcgacaatg catcgtctaa aaaggcagca tgcttatctt ccggcgcgtc tgttgccgca
    10021 tatttcgtct ctaatgagtg aactacacca atttcacccg gaagttcgtt ttctttgaaa
    10081 tagttcacta cacgggcatg agcaaccatc atattatgaa gacaggcgac gatctttgta
    10141 aaatcatatt tgattcctgg tgggaaaaca cctaacaaat attgattggt tgcaactgga
    10201 tagatttcat taaaggtact ccacaccttg acttctttga attcatgaaa acaaaaaatg
    10261 gcataggaaa caaatgcttc tattgtttct cgattcaaaa aatctccatg gtcaaacaaa
    10321 cgtttagggg tatcaaaatg atgcaacgtc acaaacggcg ttacgtttct tttcttacat
    10381 tcttcgaaca cacgatgata aaaagcgatt ccttctggat ttggtttccc agctccatca
    10441 ggaaaaatgc ggctccaagc aatggataat cttagaccat ttacaccaaa acgttcacac
    10501 aattcaatgt cctttgcata ttgatgataa aaatcacttg ccggatcggg actgaatcgc
    10561 ccttgttcct ctaaaaagtc atcccaagca acagcgcctt tgccaccttc cttagtggct
    10621 ccttcaacct gataggctgc tgtggctccg ccaaaaataa aatcttctgg taatttaagc
    10681 atgtacatct ctcctcaaat ctataatcag ttttcgctct ttctaactga ttgcattgta
    10741 aacgcatcca aaacatttgt caacaaaaac aaacacaaat aatcataaaa accagaaaac
    10801 agcaataaaa tctattgtaa caaacataaa aaccacccta aatgtttact tttgttcgaa
    10861 ttagattgac tgttgtccgt ttatatgata gattatcctt aaattagagg tgaagacaat
    10921 gttgaaaaaa gaaagattgc ttactatcgt agaaatggtc aataaaaaag gaatccttac
    10981 cgttaatgaa atcatcaatc aattagatgt ttctgatatg accgttcgca gagatttaga
    11041 tgaattagaa aaatcgggca agctgctgcg tgtccatgga ggggctcaaa gtctttccta
    11101 cactttagat caagaacttt ctcacactga aaaagccact ctgcagattc aagaaaaaaa
    11161 agaaattgct gccacagcag cctccttgat tcacgaggga gaaacgattt tcttaggacc
    11221 aggtacaaca atcgaactat tggcgacgca tttactgaac aagaaaattc ggatcattac
    11281 aaataattat ccagtttttg atattcttgc tcaagcagat ggaccagaaa ttctactgat
    11341 tggcggtgat ttccggaaaa ataccggcgc atttgtcgga ccgatcacca acgacaattt
    11401 aagaagacta aagtttacaa aagcatttat cagttcaaat ggcgttcaca acgaagcgat
    11461 ctctacttac agtaccgaag aaggagaagc acagcagatc gctctaaata attcccgtaa
    11521 gaaatatcta ctcgttgata ataaaaaatt caatcgagat gatttttatg tgttttataa
    11581 tttgcatgat ttcgatctgc tcattacgga tcgccagatc tcgcgggagg ttcaagagca
    11641 ctatgcacaa tatgtcgatc tgcaaattgc tgattcaacc gaaaatacca ctataggagc
    11701 gtaactatga aagggattat atttgatttc aacggaacaa tgtttttgga ctctcatctg
    11761 catgaagctg cttggctgca tatgatacat gatcatacaa aagatggttt atcagacgag
    11821 gatattttaa aaaatattca tggtcggacg aatacggaga ttttgaaaca tttcatttca
    11881 gagcatttga ccgcctctga gatcgccgta ttgtcagaag aaaaagaaag ctattatcgt
    11941 atgctttgct tgcaaaatga aaacgagctt cagttgacaa aagggcttga agcgactctt
    12001 gaccagctga ctgaccaaca gattccatta acgattgcca cggctactgt caaagagaat
    12061 gttgcttttt attttgatat tttcgattta gcccgctggt ttgactggga caagattgtc
    12121 tatgacgacg gctctttccc tgggaaaccg caaccggata tttttcttaa agcggcagac
    12181 aaactgagct tagctccggc tgattgtctt gttatcgaag atgcttactc aggattactt
    12241 gctgccaaac gtgcggggat cggaacaatt attgcaattg atccattcgg taaaaacaaa
    12301 acagtatttg aacaggcaca gctaaataaa gatggaacca tcaaagattt tactggtttt
    12361 tggacagagc acctgtctgc cacatccgtt aattcttaaa cgattcaact aactaccaaa
    12421 gaaaagcaac tgaaatgcac acctcctttt ataaaaaact gccagtgaaa cgacatgaaa
    12481 gatacactgc tatatcctta gtgaaatata gcagtgtatc tttgactttt tcattctttt
    12541 aaccatatga cgcgttcatc tctatctata attaacatta aaaatagtgt tttcacatta
    12601 tttctcaaac caatcttaaa aatcatcagg agtacttttt tgatatactt ttattaatgc
    12661 tgggcgtaat ggttaaaaga tttccactag ttcctctcca ccaagctgag ttgaaaaatg
    12721 cttatctgta aacaacatag acaaagagga aataaacgtt tccgtatctt tctctgtccc
    12781 aaggatatta attaattttc ctactaggta agtatcatta aacttaagat ctgccttcac
    12841 atcaaaagtt ttgataaaag cttttatcat gcgtacaatt ttgtctttat cgccataatt
    12901 gatttcacca ctcatatcaa tatagtcact aatccctgaa gaaataatgg caactgcttc
    12961 ttctttcgcc ttatcagata attcatttaa tgactgggat tgattgatga cttcttgagt
    13021 tttatctgtt acgtaatctc tcattatcct atcggcatac tccgcaaagt aatcttgaaa
    13081 aaattcttct acagggtaac caacttcgtt ttcccaacca ggagaaattt cattatcagt
    13141 acttattaaa tctattaaaa accaacccgt tctctgagtg ttgattctat aatattttta
    13201 atttcctata taaatcaaaa atattataga atcaataagc taattaagaa acaaacttta
    13261 gaaactctag aaga
//
LOCUS       DACDTH010000078        12734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDTH010000078 DACDTH010000000
VERSION     DACDTH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.079"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWK54_002407"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWK54_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9594501.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWK54_002408"
     CDS             complement(322..891)
                     /locus_tag="IWK54_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9594502.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWK54_002409"
     CDS             1077..1979
                     /locus_tag="IWK54_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9594503.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWK54_002410"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWK54_002410"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9594504.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWK54_002411"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWK54_002411"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9594505.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWK54_002412"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWK54_002412"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9594506.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWK54_002413"
     CDS             8547..8732
                     /locus_tag="IWK54_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9594507.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWK54_002414"
     CDS             complement(8794..9990)
                     /locus_tag="IWK54_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9594508.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWK54_002415"
     CDS             complement(10160..11596)
                     /locus_tag="IWK54_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9594509.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IWK54_002416"
     CDS             11889..12698
                     /locus_tag="IWK54_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9594510.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DACDTH010000079        12004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDTH010000079 DACDTH010000000
VERSION     DACDTH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.080"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..437)
                     /locus_tag="IWK54_002417"
     CDS             complement(<1..437)
                     /locus_tag="IWK54_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP9594511.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDL"
     gene            1131..1517
                     /locus_tag="IWK54_002418"
     CDS             1131..1517
                     /locus_tag="IWK54_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9594512.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1611..1802
                     /locus_tag="IWK54_002419"
     CDS             1611..1802
                     /locus_tag="IWK54_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9594513.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2316..2648
                     /locus_tag="IWK54_002420"
     CDS             2316..2648
                     /locus_tag="IWK54_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9594514.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2944..3099
                     /locus_tag="IWK54_002421"
     CDS             2944..3099
                     /locus_tag="IWK54_002421"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9594515.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3375..4715
                     /locus_tag="IWK54_002422"
                     /pseudo
     CDS             3375..4715
                     /locus_tag="IWK54_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4956..6572)
                     /locus_tag="IWK54_002423"
     CDS             complement(4956..6572)
                     /locus_tag="IWK54_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9594516.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6690..6908
                     /locus_tag="IWK54_002424"
     CDS             6690..6908
                     /locus_tag="IWK54_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9594517.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            7103..7564
                     /locus_tag="IWK54_002425"
     CDS             7103..7564
                     /locus_tag="IWK54_002425"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9594518.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7757..7996)
                     /locus_tag="IWK54_002426"
     CDS             complement(7757..7996)
                     /locus_tag="IWK54_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594519.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(8020..9543)
                     /locus_tag="IWK54_002427"
     CDS             complement(8020..9543)
                     /locus_tag="IWK54_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9594520.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9561..9683)
                     /locus_tag="IWK54_002428"
     CDS             complement(9561..9683)
                     /locus_tag="IWK54_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9594521.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9713..10696)
                     /locus_tag="IWK54_002429"
     CDS             complement(9713..10696)
                     /locus_tag="IWK54_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9594522.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10720..10869)
                     /gene="rpmG"
                     /locus_tag="IWK54_002430"
     CDS             complement(10720..10869)
                     /gene="rpmG"
                     /locus_tag="IWK54_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9594523.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10890..11267)
                     /locus_tag="IWK54_002431"
     CDS             complement(10890..11267)
                     /locus_tag="IWK54_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594524.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(11299..11478)
                     /gene="rpmF"
                     /locus_tag="IWK54_002432"
     CDS             complement(11299..11478)
                     /gene="rpmF"
                     /locus_tag="IWK54_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9594525.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(11493..11762)
                     /gene="rpsN"
                     /locus_tag="IWK54_002433"
     CDS             complement(11493..11762)
                     /gene="rpsN"
                     /locus_tag="IWK54_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9594526.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
BASE COUNT         3860 a         2281 c         1857 g         4006 t
ORIGIN      
        1 aatagatctt ctatttttcc agtaaaaaaa taatgaacta aaatcagaat atctcgaatg
       61 tctcctaaag aatacaaagt caaattttgc aatgaattat agagtcgttc tctatcagcc
      121 attgcaacat tcaaattgtt catttttagt ttaaaaaaaa ctttatcaat attatttgct
      181 ctaaatggac caaataagta atatctggtt ttaaacatgt atagaagaaa catctcatta
      241 aaatcaccag taaagaaatg gaaatcttta gtttttttaa tattatcagt tagtaacttt
      301 ttataatcat aaaaaaaata agtagttcta tctgagcgat actcttttat aatactgaat
      361 tcggaattaa aaaccacgat aggtaacttt aaacaaacat gaagagactt taataagact
      421 tctttatctg aaaacacttt tgtttctctc tttcctttct acatcattaa atagtatttt
      481 atacgttatc taaaatacta ttcctctgtc aaaataagct aacttacttc attataaaat
      541 atccacttat cgctttcatg tatattccac cgtaaaaagt acattttatc accgagaact
      601 catcattttt catcatgatg aatactattt acatatatct agttcaaaga caataattat
      661 tatgatccgt tattatctaa gcactataca gataatagga atttttcgtt tctaaatatt
      721 ccaatttggg aatccaataa aaattttcgt tcaaatacgt gtctattcga actcttttct
      781 agttgttaat tatgaagggg gaagaaataa aaaatacaat ctatacacga ataagaatat
      841 tatccaaaag gaggttttag aaaagattta ttatacgtaa aaagctcaag acattttctt
      901 agccataagg acatgtttga tatttaagaa gatatatgaa ttttcttttc taagtataaa
      961 gataactatc cattatcaac taaaaaaaca tttttgaaaa taaacatcag cagtcctatc
     1021 aatgcatgtg agcttataga agttattcgc agcgcagcat aattgcatta gcttcttttc
     1081 gtgttttttg cctcttttcc ctttaaatct taatggagga ggtgtgcaat atgtcagata
     1141 acgacgaacg cgtaaatgcc cagttgattc gattctttga aaaggtcatt ttgaacaccg
     1201 ctaatactta ttataaaaaa caagaagaaa ttcaaaaaca tgaacaactt gatcaactcc
     1261 caccatattt gtttactaaa gatactataa aatttaaaca accgattaat gttttaaatg
     1321 tgactctcct tgttgaagat ataaaattag caaaaatcct tcctttgttg aaagaacctg
     1381 aacaaatttt tcttatcgaa aaatttatct ttgataaaac tgataaagaa attggaaagg
     1441 ttctggggat tacaagacag ggtgcgacaa atttaaaaca tcgactttac aaaaaaattc
     1501 aaaaaatgct ggtttaatat ctgcataata actaattttg tatgcttctt tgctcgttct
     1561 cctccttccc aaagaaatag attataagtc catgggaaga aggtagattt atgaattctt
     1621 taaaagaaga tttcatttta gcaaaggctg gaaatgaaga agctgttgag gcaattctca
     1681 aaagatttag ctctttgatt cataagcaat cctggagaac tggaaaatat gatcaagatt
     1741 gttatcaaga atgtatgctt gcaatatatc tagctatctc gaaattcgaa attaaagaat
     1801 aactcaacta agggagtctg tgacataagt aaaaagatag tttcaaattt cgaacaataa
     1861 atttgaaact atcttcttta taattataaa gtatcgattt tacagcgttc tataatgatt
     1921 catagaatct aaaacgaatg attttaatta tcaacgtttt ttaattcgtt tttagcttaa
     1981 aaataacttt tgtcacagtc tcactcctaa aattacttta tttacgtatg aagtgggcag
     2041 gaggtcgaat ccagaactgg gctatggtac ttcaacgagg acttctcctc tagaattgaa
     2101 aagcatgcga tttaccttcc gtaacgacgg tagatttatt caactatgtg tccaatcaaa
     2161 atttgtctga tttcaaaaat agtcaagaga attttggttg ttaaaaactc ttatctatca
     2221 acttccacag tttagttgac aaaccctgat actaagttgg gctatgatac acttttcttg
     2281 actatcagtt acgttaccgg aaggaatgaa ctctattgag aaaaacatat gatcgtaaat
     2341 ttaaactaaa gatttcacag gatatccttg agaaaaaagt aactaccaag aaaattgctg
     2401 aagaatacaa tatctctcgt ccaacaattt cccgatgggt ttcagaatat cgtcgatacg
     2461 ggaaaaatgc ttttgctgga caaggaaaaa gattacctga taaagcagat ttctacattc
     2521 ttgaacagga aaataaacga ctaaaagagg aagacgagtg ttctacgaac acaaagatcg
     2581 atacggatct ccagggatca ctcaaaaact ttacgatgaa ggaatcgaaa tcaataaacg
     2641 tgttgtagct gttcttatgc gtgaaatgaa tttatgcaca agaggatttc atcgacgcaa
     2701 atcttctcat ggtagagaaa aaacaatcaa agaaatggta aaaaaatgtt gaatcgataa
     2761 tttgaacaga accaaattga tgctgtttgg gtgatggata ttacttacat tccatgtaat
     2821 gacggtcgcc tctatttgtc cacctatatt gacttagcaa ctcgtattct acgttactat
     2881 atggtagatt cacacatgaa aaaagagatt gtcgtccgtc cattacaaat ttattaagga
     2941 gaattgccta cggtaattca ttccgataga ggtagccagc atcggtcttt tatttatcaa
     3001 gagctgttga acgaccacca catcacacat tcagtgagcc aaactgggac tcctgttgac
     3061 aatgcagtaa ttgaagcgtt tcatcgatca atcaaatgag aattaatcga ctccaacaaa
     3121 tataaaagta aaatagagat gaaggtgtta atccaagatt atttagaaga ctactaccca
     3181 aacaaaagaa tccatacaaa atttatgatg actccttgac aatttgaagc gaagaaaata
     3241 agggatctat aatatctagt gttggtgacg ccttctgttg ccaatacttt ttcttttttc
     3301 taataaacgc aaacagacac cttaaaatcc tttagaataa agtcgacgaa aaccattcaa
     3361 aggagtgttt caagatgtcc tattcagagt ttaccaaaga gttactagat attctagacc
     3421 taaatcttat ctttcatgaa gatgcctttc gcaaagagcg aatcaatgac gaaacatgtt
     3481 ttgtttttga tggcacattg acctatcaac cggaggaatg ctttcatcgc cactatcaaa
     3541 atgaacaaac gatcattaaa tagggctgga aaaaagtatc catcttattg aatgatgtca
     3601 gtaactacaa gaccattctg cggattaaca aacagcgctt caaatgtaaa cactgtggaa
     3661 agactttttt agcagaagat tcagttagtg atcgtcgctg ctccattgct cgcagagtca
     3721 aacaagccat tcttgagcta ttaagcgaac ctctttctat gtctttgatc gcacgtatga
     3781 agcacatttc tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccattt
     3841 ctttgagcca gctgttacca gaagtggtgt gtttcgatga attcaaatca gtgaaaaatg
     3901 tctcgggagc catgagcttt gtcatgatgg acggcaagac ccatcaactg attgatattg
     3961 tggaaaatcg gcaactcaat cctttacgcg actatttctt gcgctaccct ataaaagtcc
     4021 gtgaacaagt gcgtctagtg gtttcagatt tctatacacc ctatcgcaca ttggtgaaag
     4081 agtgttttcc gaatgcccga atcgtcgccg atcgctttca tattagccaa cacattggtc
     4141 gggcttttac caaccaccga attcaagtaa tgaagacctt taaaaaaggg gatcgtcggt
     4201 cgaagcactt aaagaaatac tggaaactac ttcaaaagaa tgcgtgggaa ttgaaaggtc
     4261 agcatcgcta ctggcgtccg tcatttcgag atcatctgac agaagcagag atagtcgatc
     4321 ggctattatc ttatgatgat tccttaaagc gaggctacga agtctaccag gattttcttt
     4381 ccgttatcag aagacaagat gttcctgaat tcgtcgcgct cctaaaagaa gactacaagg
     4441 agttgccaga gcactaccaa ccagtgttca cgaccttcaa gaaataccaa acagaaatca
     4501 aacgagcgtt gcgcgttccc tattccaacg gccctataga atgtttgaat aaccatatca
     4561 aagtactaaa acgaattgcc tatggctata gaaactttca aaactaccga gaacgaatct
     4621 ttttatatcg aggaaaatat tttaagaaaa ctaagaatac tacccaactg actaaagcca
     4681 gaacaacaac acggctagac aagatagctg tctagccgtg tgagtatctc gattattcta
     4741 ttgaatttct tcaccaacac tcattgacga agagccaaaa taagtaatct aaccgactgt
     4801 taactaaacc attgcaggtc ccgttaaaga tacttgtgcc tgttgtcacc gcttcttttt
     4861 cttgtccaca agcgcttaac acacttaata aagcccctgc tattattaat aataatatct
     4921 ttttcttatt aaaaacctcc tcatctattt ccagattagt aaatgtagta atctgtgctt
     4981 tcttttttct caatactaat aactaactta tggggtaaac aaatactcgt ttgaccgggt
     5041 tttgagatcc atccagttct aaccgcgatt tgatcaggac tattatcttc tttaactcga
     5101 attcttccat ctttaatctc aacaatatta tattgtccct tcgcaggatg ataattcacg
     5161 agtttatgat tgatcttatc caaatcgaag cgatccattt ccttgccatc aattcgaacg
     5221 atggcatatt tgatagcatc ttcagttgtt tcattctcct tttctataga gaatacaaat
     5281 aagggaataa aagaggtaat aatcaaaatg ccaataatta tgacatcgcc tatcttgaca
     5341 ccagcttttc ttaaaaattt cattaacgat atctggctaa acatattaga ctttttccat
     5401 ggaaatggtg aaatttttcg gaaattatct gcattaagag aatcagttaa acaaccctca
     5461 agttgttcct taatacattg ttgatccatc ttcataccaa taaaaactag tttattaatg
     5521 cgttcaccaa acaacggatg ccaggcttcc ttcaattcgg gatttttttg tataatcatt
     5581 tgacactcaa ttttaggtaa actagctacc caataattta atgattcaat acttttatag
     5641 ctacccgatg tttcaaagat cgtcgaaaaa tcactatgtt gggcaaacca aagaaaccct
     5701 tttgctcttg taatattgcc aggaaaattt ttatccgtaa gatgggcaaa tttttgtgcg
     5761 tcaaggagta accgatttcg atagataaaa cttgagatac catattcttc tgtttcagga
     5821 atatgatttt gatagccaag ctctaactct ttgaaccagc ccactgaata ggaagtctca
     5881 tctatattaa aaagatttgt atttagaatt ttcgataagt ctacttcacc tttaattgtc
     5941 ctaattattt tggcattgga ctgtaactta gcaattagac cgttaatttt gtaaatcgtc
     6001 ttggttgaca ctaagtcact attatttaaa agtaatatgt tacaacactc gaattgatca
     6061 attaacaagc cacgaatatc ctgttcttct tcaactacat taccttcagc ttttctttcc
     6121 tgaatctttt cacccgtttc gaaattatcc caaaaacgtt ttacatccac tactataata
     6181 atggcaccaa tctctaatcg ttgggtcaaa tcaatgtcta aagggttttt agctaatata
     6241 attgcttgaa taattggaat tggtttgctg attcctgaag cttcaataac aatttgattc
     6301 aagtcagaaa attgcactaa ttgatctagt tcaacaatca aatcttcgcg taatgtacaa
     6361 caaatgcatc cattcggcat cgaaacaagt ttttctccag ttcttttaaa acctttcttg
     6421 tcgactaata ccccatcaac atttacttcc cctatgtcat ttacaatgac agcaatttcc
     6481 tttccgtgat caccgtacag aatatgattt agaagtatcg tttttcctga accaagatag
     6541 ccagttaaga ttgtaatagg tatttttttc atagggaacc tctttctata ccttgaattt
     6601 acactttaag tgcaacacat aaaaatgagt cataatcgat atccaactag tataagtttg
     6661 tcgagaacac tcctagaagg aaaataatta tgagccagta tcgtcatctt actatactcg
     6721 aacgagaaag aatctttctg cttcacgaag aaggattttc tattagtata attggcaacg
     6781 acattcagca tagcccgttt actattttac atgaactgag aagaaacaag aaaaaaacta
     6841 ttcaccttcc aaagcggaaa aaaattatgc ttatcgaaga aaaaaatgcg gacgttatca
     6901 cattctaaat gatccgtgag tttggtcaat tgttcgtcat ttatttgtcg attttcagtg
     6961 gtcgccagag gaaatctcga atcgtttgaa gcatgaacat atggactttt caattagttt
     7021 tatcacgatc taacgtgcaa tttgcaatga tcttttcgat acagaacccc tctcacacgg
     7081 caataaaggc ctgatccgtt cattgcacca tcgaggaaaa acaagacata caaaaaatca
     7141 tgtcgagtct cgtggaaaga tttgccttac tcattcgatc catggccgtc caaaatccgc
     7201 agatgataga agtgattttg gtcattggaa agccgatatt gtagctggca aaactggtgg
     7261 tacttgttta gtgactttga cagacagaaa atcatgcttc cttttagcag gaaagaaggt
     7321 ccccagaaag aaagccgctt atgtgagcga gaaaatgatt gaacttttaa gcagtattcc
     7381 aaatgaggcg gtcaaaacga tcactccaga tagaggaaaa gaattctcca atcatttcgc
     7441 agttacagaa gcattgaata atatgccctt ttattttcca gatcctcatg cccttggcaa
     7501 caatgaacaa attaaaatac gaatagactc attcgagaat acttgccaaa atcgaaagag
     7561 atagctacgg tagataactc aacgataaat cagatgattg aaaaactgaa taacagacct
     7621 agaaagtgtt tagattggag aacaccttat gaagtcttct ttgataaagt gttgcgcttg
     7681 atttgacaat tcaagacata ataaaaagca gatcaaatcc aaataaggga aaaatgatct
     7741 actctggtat tatcaattaa ttggccaagc tattatgccg ttttgctaaa cgtctacttc
     7801 ttccagtatt gaccttgata aaacaacaat caggatcccg ttttaatact ctatgaactg
     7861 tgtcaatact aacatgtaaa gttgcagcaa tatcagctaa tgtactccca tttaatctca
     7921 tttgctttgc aaatcgataa cgccgataag catcaatatt cttggaatcc atagacaata
     7981 atacttttct taccatgtat ttcctccaaa aattgaattt taatgagcca tcatttcttg
     8041 agcaatttca tgtttagaca atgaagctgg atagaaagta ggccaattat gcaattcatt
     8101 atagagtttt tcttgacttt ctccaccaaa gaaaatatgg aaatgctctg ccttacttgg
     8161 tgcaattcca tgatcactaa attgaatata tttaaaacga ccagcacctg catcatcagt
     8221 ttcaaaatta aaacgaacac cacggtttcc tttttcataa ttcaaaattt tgtatccaac
     8281 atatttgtat gtgtattgat gatgttcacc gttgactaca aaatcaatgg tgttgtcttt
     8341 aatgttgata gtatcaatat ctgtcttata gccagtagta taataatctt tgtactcggc
     8401 agctgtcatg tctttattga tttttgcttt gtaatcgaaa acttcatcca aaattccatc
     8461 ttttaacaac ggataaacag attgccattc gcctgtataa tcactcaatt cgcgatcctt
     8521 cacatcggca tctttaaagt acccattagc aactgtcttt tcatccttcg cttctttttc
     8581 tggaactacc tcttcaccag caaccatagt tgttttttct aacgctttta gattgtcttc
     8641 cattactgaa acataatctt caccattatc catttgttct tttgtaagac tttcaagagg
     8701 attcagtact tccaatttta tcccagcttc gttggccaaa gtttttgcaa ttttatcatt
     8761 agcattcttt tcaaagtaaa tataattgat cttatttttt ttaacatact cttttaattc
     8821 agctaatcga ccagaagatg gctcttcttc aggacttagt ccagcaatag gaacttgttt
     8881 caatccataa tccaaagcta aatatccaaa ggctgcgtgt tgcgtcacaa aacttttttg
     8941 tttcgcttct ttaagactag tggtatagtc agcatccaac cgtttcagtt tagtcaaata
     9001 cttttcagca tttgtctcaa atacttttgc ttttttcgga tacaatttaa ctaactgatc
     9061 cttgatattt gaaacttcct taattgccat tttgggtgaa acccacgtat gaggatctag
     9121 ttcatgatgg tgatcttctt ctccgtgatc atgatcttct tcttcactac ctggcaataa
     9181 aatcatgtct tttgttcctt ctactacatt aggcccagct ttcttccaac tttctttagc
     9241 ttttggtacc catgattcca tgttttcgtt atgatataca aaaacatcag catcatgagc
     9301 ttttgccata tccttagctg agggctcata gtcatgaggt tctgatcccg ctggaaccat
     9361 taaatccacg ttcgcttcat cgccgacgat gtttttggtg aaatcataca ttggatagaa
     9421 tgttgttaca atttctaatt ttccatcttt atcattcgat acttttgcat cattagtgct
     9481 ggagcaagct cccaataatc ctagagctgc tatcccaata agtcctacac tgattttctt
     9541 cattcttttt ccttccttaa ttatgctaat ttcttcattt tatttcgttt atgttctttg
     9601 ataattttta atgccctttt tctagtttta atattcgggc ttttaagtcg tcgatatgca
     9661 ctagatatat ccgttttctc cattagaaat tacccccaat cactacttat cattatttgc
     9721 tttccgccag taatttatca aaactctgtt tgatcctttc ttcatccaac atttttccga
     9781 ttagaataat agtggtttgg tctttttgtc gagtggtgta ttgaaaatta acctgttcgt
     9841 ttacctcttg catggcaact ataaaatcct gctcttgtaa agcaatcaat cctttaaaac
     9901 gatacaggct cccttggttg gttaaaatca gccagtcaat ccattggagt aaaaaatctt
     9961 tttttatgtc ttgatctgaa gttaagagaa ttgatttaaa ctcatgtact tgatggtgtt
    10021 gatgatagta tttgccatta tagtgatttt ctgatgaatc actttcaaaa actaagggat
    10081 gattaaattt tttcaaatta taaaaatcct ttttttgaat cgtttcacta tgtgtgaaca
    10141 ttcgaatatc ggataaaggg ttaatacttt tgatttctct ctgtaatttt tttagtgcag
    10201 ataaattcaa gttttgtttt ctagacacaa aaatgcgatc tgccatgaca agttgcttaa
    10261 gggcttgtgg ttctctttca ataagttcaa aactgtcact atcaataaca gtcaataaac
    10321 tatcgatata tactcttcct cttagttgag gcgctgttaa aagtgtttga atgattggtt
    10381 ggggatctgc tatacctgta gtttcaatga tgatttgtga aattggttgt tgatgttctt
    10441 ctattgtttc cagtaaagaa atcaatattg ctaataaatc actgcgaaga ctgcaacaca
    10501 tacatccatt atttagttgt aacacccgtt cctcgctatg aactaataat ttgtgatcaa
    10561 tcccaacttc accaaactca ttttcaatta taataatttc ttccctgggg tagggtgaga
    10621 cttgcaaggc ttgattgatt agcgttgttt ttccagcccc taaaaaccca gaaacgattg
    10681 taactggtat gcccattttg tcaccttttt accacttagc tatttcactt ctttaaaaat
    10741 tgcccgtctg cgcaattttg gcgaatactt ttttaattct aaccgttcag ggttattccg
    10801 tttatttttg ctggtgagat acagacgttc tccagtttcg acacattcca aaataatatt
    10861 ttgacgcata aatcttcctc cttcaatact tatcgatctt caagatatgc ttgaatttgt
    10921 tgtgcatctt caaaagttaa ctcgccagat tccaattgtg aaactaaatt ttctttcata
    10981 tcaacaggta tctcccaata accatcgata cattgaaata actcctcgtc atactcttca
    11041 aaaattggat agattcttaa aaaatcatca aatgataagc cttggtcctc atagtaatct
    11101 ttgactaatc cattttcaat tgcctgttgg gtcggtaaac ccgtttcagt aataattcct
    11161 ctctctacca tcgaacgata aaaagtgcat ttatctagcg attgctgggt cgtttcaaga
    11221 taatacttta atccttcttc aactaaaggt gggtaaataa tttccatttt gattccctcc
    11281 taaaagttat atattaaact attttgaagc tcctttaatt actttctttc cattatagta
    11341 cccttttaaa gatacatgat ggctacgtct ataatcacca ttcgcttcgt caaatgaaat
    11401 ttctggtttt actacagttt gatgagttct tcttaaccgt ttcttcgcct tagatgtttt
    11461 tcttgctggt actgccatga tatttcctcc ttttaccaac ttgctttttt tactccagga
    11521 atcaaccctt gatgggcaag ttctctaaac ttaatacggg acataccgaa ctttcgcata
    11581 taccctcttg gacgaccatc cgtctgatca cgaagcctca gacgattcgg atttgaatct
    11641 cttggcaatt tagcgagagc ggtgcgatct cctgcggctt ttaattcctg acgtttatct
    11701 gcatactttt caatcatcgc catttgtttc tttgctttag caattttcga cttttttgcc
    11761 atataccttt atactccttt tattttgatt tagacacctc aaatcgtaat cgttacgttt
    11821 tgacactgat actttaccaa caagagttgc tcttgtcaac aataaataac atgaaataaa
    11881 actcatatga atagataact atttgaaaac gaatttatcg gtacatagag ataatcaaat
    11941 catttaaaag aatatgcagt ttaggctctg aaaaataaga attcactttt attagtccaa
    12001 tcaa
//
LOCUS       DACDTH010000080        11667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDTH010000080 DACDTH010000000
VERSION     DACDTH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.081"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(37..216)
                     /locus_tag="IWK54_002434"
     CDS             complement(37..216)
                     /locus_tag="IWK54_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594527.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="IWK54_002435"
     CDS             complement(597..1304)
                     /locus_tag="IWK54_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594528.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="IWK54_002436"
     CDS             complement(1297..1734)
                     /locus_tag="IWK54_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594529.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="IWK54_002437"
     CDS             complement(1749..2000)
                     /locus_tag="IWK54_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594530.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="IWK54_002438"
     CDS             complement(2000..2206)
                     /locus_tag="IWK54_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9594531.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="IWK54_002439"
     CDS             complement(2643..2903)
                     /locus_tag="IWK54_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594532.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="IWK54_002440"
     CDS             2978..3181
                     /locus_tag="IWK54_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9594533.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IWK54_002441"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IWK54_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9594534.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="IWK54_002442"
     CDS             complement(3810..4709)
                     /locus_tag="IWK54_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9594535.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="IWK54_002443"
     CDS             complement(4722..5315)
                     /locus_tag="IWK54_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP9594536.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IWK54_002444"
     CDS             complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IWK54_002444"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9594537.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="IWK54_002445"
     CDS             complement(7771..9231)
                     /locus_tag="IWK54_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9594538.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="IWK54_002446"
     CDS             complement(9292..11664)
                     /locus_tag="IWK54_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594539.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtc
//
LOCUS       DACDTH010000081        11623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDTH010000081 DACDTH010000000
VERSION     DACDTH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.082"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(119..1249)
                     /locus_tag="IWK54_002447"
     CDS             complement(119..1249)
                     /locus_tag="IWK54_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9594540.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(1790..1996)
                     /locus_tag="IWK54_002448"
     CDS             complement(1790..1996)
                     /locus_tag="IWK54_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594541.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2012..2626)
                     /locus_tag="IWK54_002449"
     CDS             complement(2012..2626)
                     /locus_tag="IWK54_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9594542.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2773..3456)
                     /locus_tag="IWK54_002450"
     CDS             complement(2773..3456)
                     /locus_tag="IWK54_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9594543.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3565..4023)
                     /locus_tag="IWK54_002451"
     CDS             complement(3565..4023)
                     /locus_tag="IWK54_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9594544.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4107..5870)
                     /locus_tag="IWK54_002452"
     CDS             complement(4107..5870)
                     /locus_tag="IWK54_002452"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9594545.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6091..7551)
                     /locus_tag="IWK54_002453"
     CDS             complement(6091..7551)
                     /locus_tag="IWK54_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594546.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7759..8685)
                     /gene="glsA"
                     /locus_tag="IWK54_002454"
     CDS             complement(7759..8685)
                     /gene="glsA"
                     /locus_tag="IWK54_002454"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9594547.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8708..10135)
                     /locus_tag="IWK54_002455"
     CDS             complement(8708..10135)
                     /locus_tag="IWK54_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9594548.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10166..11380)
                     /locus_tag="IWK54_002456"
     CDS             complement(10166..11380)
                     /locus_tag="IWK54_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9594549.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3521 a         2404 c         1916 g         3782 t
ORIGIN      
        1 taatatgatt ttttgagata ctttcattgc gaattcctct ttctaaaaga acctctcgct
       61 tgaaaaataa aagattgtac tttatgataa ttcaaactga gagtttctca gtttctcttt
      121 attgtttttc aggtcgatag agcgccaact cttcggcctg tatctttgaa gaaatattca
      181 acgtgattcc gattcctaaa gaaaggatca gataacttgt tcctccgtag ctaacaaatg
      241 gcaatggcac accagtcatt ggcatcagcc ccgcgatact tcccacgttc attatcgtct
      301 gaacgaatag caaagttccg aatcctaaaa gaaacaatcc agcttgctga tttttacagc
      361 gactgcttaa acaaaagatt ctcatgcata atgagaacaa taagaaaagc acacacaatg
      421 caccgattag cccaagctct tctgtaataa tcgaaaaaat aaaatctgtt tctgtctctg
      481 gaagataccc tttttttgtg atactatttc ctaatccccg cccaaatatg ccaccattat
      541 atagtgcata aaacgaatgg gtcatttgat agcccgcacc atgactatcg ataaaagaat
      601 ctcttaaagt cgcgatcctt tcataagcat gattaaacat ctgtggaagc caacctttat
      661 ttcctaaata taataccccg cctgctgctc caatcagcaa tgcagaaaaa gtaacgatta
      721 gatagatccc ttttttaaat ggaaccgccg ctgcccaaaa tatgacccac gcaatcgaaa
      781 ggatcatcaa tgctccagca atctttggct gaaataaaat cagaaaagtt atacctactg
      841 taatcaggaa tggttttttt agatcttttg ggctactatt tccgtcacga aaaaaccagc
      901 ttaaataaaa aatcaaaaaa agatttgcca gttcagaagg ctggaattga atgccaaaca
      961 gagagatcca acgttgtgcg ccgttgacag tgacaccgaa tatccctatt cttactaata
     1021 ctaaaaagaa aatcgataag gctaaaccgt atcctgctat tttagggtga agtaaatagt
     1081 gtagtttgac tgaacgagct aagaagatca cgctccaact caaaaatatg aaaattagtt
     1141 gacgcaataa tagacttttt gtattttcat cggcttgcaa taaacgataa gaactggcac
     1201 tgtatacttc taaaagaccg atcatggaca aagtcaaata aggacccaaa atccaccaat
     1261 cgatcttttt tcaccgcatc tcctcctcac acagcaatcc acttgtcagg tgaggagcga
     1321 gtttgtctca tcaaataagt cagtcacaaa aatataaaga aaaggtcaga aaccttttcg
     1381 aacaagaatt ctgacctagc atcatctcat cgtccgagtt ttagcactat acaacgtaaa
     1441 gaaaacattc ttagctattg gaaaaagcct taatccaaca gtttctgttt tacccattgg
     1501 cgactttcga cgtttctggg caacagccta tgtttgtata ggagcctcac ctaacaaggt
     1561 tattactata ttctatttac gagttcatga tagacttttc ctaatgatta ggcaatggaa
     1621 tttgaaatga aatagtgaaa tttaacaatc tttaatattt tttagaaaaa aataacaaat
     1681 aagttgaaag aatcccgttg caaattcttt cagttgcacc aggattcttt gataattttt
     1741 tagttttatg tgttttttta tatgtttttt cacttttcgg aataaatatt tatttgttaa
     1801 agtaattaaa caatccgcta atcgtttgat ctggcatttc tatttcctta ccatctaaag
     1861 taacagtagt aacgacaaaa tggtcttgca atggcggaac ttctaagtac tgcccttgca
     1921 gaaaatcatt cactgtttca tattcttttt tcatttcttt tgaatctgtc gatggtttat
     1981 agcttatttt taacataact agtgctcctt tttattcggg acgtgcaatc gtgaaaattt
     2041 ctcctattgc tgcataatca tttcctgcca aacggcttac agcattcaat tttcttggat
     2101 cgatccttcc tgtcttatca tcatagactg tttcatttaa atgatatttc tgtacttcta
     2161 gcaaaaataa atcagctgtt actttatttt tattccgtat ctcgacatgt tcatataagg
     2221 atgtttcaaa acgaactttt gcttctgtca atgctggtac tgaaatgatt actgaatcag
     2281 ttaaagtcaa gtcagtcagg tacagttcgc tttcctcatt aggcagattg gctgctgttt
     2341 tatttacttc ttctacgttg tcctcatcaa ggatatggat aacagcttct cttgtctcaa
     2401 gaatattctt tgctgtgtct tttactgcat cttgttttct ctggatcgcc aaggataaga
     2461 tgggtcgatt cgaactgacg atattgaagt aactgaaggg tgcgatattc actatatctt
     2521 ttgctgattt agtcgttact actgctactg gtctagggat gactgaacca atcagcaact
     2581 tgtaattttc acgttctgtc aattctgaag gactccgttc aaacatggtt tcgccttctt
     2641 tctataaaat tcgttaaaaa gcagatcttg aagacaaaat gaaaagtaat gcttttttca
     2701 tcttcttaga atctgcttta aactatctat caatttttct tttttgtcaa gaatctgtgc
     2761 cgtgcgtgtt ttttagttat ttgctttact tgcggcttcg acatatcttt tagtgatcaa
     2821 ttgcggacgg gtgataatag atccaactac gactgaaaaa gcacctaatt catatgcttt
     2881 ctctacttgt tcaggagagt cagtatgtcc ttccataatt actgggatat cgacttgttc
     2941 tatgatttga ttcaataaat caaaatcagg tgtctgcgtc tttcgtgaat attctgtata
     3001 gcctgctaaa gtcgtagaca aaagatcaaa accatcttct tgcgcttgaa gcgcttcctc
     3061 aaaagtcgaa atatctgcca tagctaaccg accgtgttcg tgaatcattt ggacaagttc
     3121 agcagttgtt ttttctgtcg gacgtttacg agaagttgca tccagtgcaa tgatttctgc
     3181 ttgcgtctcc aaaagttctt ccacttcttt ttctgtcgca gtaataaaca cttctgaccc
     3241 atcgtaatct cttttgataa tccctattac cggtaaatca acgattcgct tgatttcatt
     3301 gatatcttct gtcgtatttg ctcgaatacc aactgctccg ccttcttttg cagccaaagc
     3361 cattctcccc ataataaaag aactgtgtag cggctcgttt tccaaagctt gacaagaaac
     3421 gacgactccg cctttgattt gttccagtgt atccacaata tctcctccag taatgttata
     3481 taaaaataca gcttcatata aaaatgtaac ccttttctat tcaggagaca ataatgagct
     3541 atttctttca gcagaccttt cttactaaaa gaaaattact tcaaattctt cgtatacgac
     3601 cagcttctct tctgtaaatt ctatgttcaa cgcttcttta taagcatgtt taaaaaactc
     3661 agcatggact ttccgccaat aagctaatgt ccgatcccct tctccttctt tccaagcatg
     3721 ctctttcgtg acttcactaa aaggcaagat ggtcacattt atatttttga tgatacaaac
     3781 tggttcattt tttccattca ggaccacgtc taattgacca acttttggta aaggctcatt
     3841 ctcttgttta tacagatcgt aaccacttga tgtaccagtc tttatctttt ttacaactag
     3901 ttcgctcaac tcgtctgcca ttttttctgt atccccaaat gaaaagaaac ctaatttttc
     3961 tgattgataa ctaggatatt tttcttggaa ctttttgaca tattcttcta cttgctgata
     4021 cataacatcc acccctttgc tttttataat gaaaaaacca tccagatact agtataaaca
     4081 attatctgga tggtgagcat aatgaattat tgaatattca agacacgcag actatcttca
     4141 acgatttgtt ttggtgttaa atgataacgt tcataaagct gccctacagg aacgctatcc
     4201 gtgaattctt tctctgcacc aaaatttaat acgttcattg aagaatcacc ataataacga
     4261 ctgatatttt cgccaaatcc tcctgacaaa ctaccatctt ctaatgtgat gactaattcg
     4321 tgtgtttctt ttaatttatc taatgtttct acgtcatttt ctgtgatcgt tcctggattg
     4381 atcaacgttg cttcgattcc tttttttcta aaagctttta ctgtttcttt tcctaattca
     4441 agaaaaccgc ctaaagctaa aattgctact tttcctccat tatttactgt ttgataggct
     4501 ggaatactat aatcatttgc gatagctgta ccatgttcta caccatgtgt aggaattcgg
     4561 attgctacag gctgcgctgt ttgttcgaac gcccaatcta gcatagagat catatcctct
     4621 tttgttgctg gagcaagata tttgatgttc gggatacttg ataagtaagg aatgtcaaat
     4681 gttccttggt gtgttcggtc actagctgag atcgttccgc caccgactaa aataatcact
     4741 ggattctcgt tgatcgctaa atcatgtgat aattgatcat atgcccgttg caagaaagta
     4801 cttgattgga agacgactgg acgtactccc tgagaagcca aagcagaagc gaaagtgata
     4861 gagtgctgtt cagcgatccc cacgtcaaaa taatgatctg gatgtttttc ttggaatgtt
     4921 ttcagtccaa aggcgcctgg tatcgctgca ttgactgcgg caataggagc tgtacttgtt
     4981 tgcattttct tatccaaata atccatgatg atcatattaa atgtttctcc agaagttact
     5041 tgctttgcct ccccggtgga cagatcaaat ggtacatgcc aatgatattt cattttatta
     5101 tcgattgccg gctgataacc gcgacctttt tccgtatgga cgtgaagtac aatcggatga
     5161 tcaatatctt ttacttcttc aaataaacgg atcaccgttt ccagatcgtt tccattatcc
     5221 acgtaacgat aatcaaatcc cattgcttta aataggttat tgctgctttt accgttggtt
     5281 tgacgaagct cagctaagtt tttatataaa ctgccatgat tctcatcaat agacatctca
     5341 ttatcgttta ctaatacgat tagattggat tgtaaaacac caccattgtt caatccttca
     5401 aaagctaagc caccacttag ggagccgtca ccgataaatg ctaccacatt tcctgattct
     5461 ttcttcagat ctctcgcttt agctaaacct gttgctaagc tgatagatgt agatgtgtgc
     5521 cccactgtaa aatagtcatg tttactttca tgttggttgg aatatccgct cacttcacgg
     5581 taatgtcctt tttcaaaacc attttttcga ccagttaaaa gcttatgacc ataagactga
     5641 tgggaaacat cccaaacgat tttatccttt ggtgaatcaa atacataatg aaacgcaatc
     5701 gttaattcta ctacacctag attcggacca acgtgcccac ctacatcgct gtccttttgc
     5761 aagatttttt ggcgcatctc ttctgccaac tgtttcattt cctcaacaga taattttttg
     5821 acatctgcgg ggctattgat tgcatccaat acatgtaata ctgtactcat aatacattct
     5881 ctcctccgta ctatcttctt ttaatgaaaa tagatttgtt acactttatc tattctacta
     5941 cttagagttg actctaagtc aatagcattt taaaaaaatt tcagagaaag cttgttatat
     6001 aagtagatct tcgcacgaat caggactcaa ataaaaacct tctacagaca attaaaaagc
     6061 tttgtagaag gtctcctaat taaggtctca tcaagaaata tgttttttta ataggtgaag
     6121 ttcctgagca atatgataca aatggatgtt tgcacctttt aatgagattg gatagatctt
     6181 tgtcttttca gtttctaatc cattttgata gctagcagca tcgcacaaaa ccactctttt
     6241 tttcgcacat agacattcat caggcaattc ttctaatgaa cgaaataaaa taggatttat
     6301 ttcagcgatg ccttcttccg catacaaatg aaattgggtt cgataaaaaa attgtttgac
     6361 tgaactttct gaaacaacaa aaatttctat ttcctgtctc cccgcttgtt cactgttgat
     6421 caaagaaaga cttttgacga attttcgaat gaaattttca ttccatctta gttggatttg
     6481 atttttcttt ttccactctt ccaatatttc tacaattttt ccgtacagaa gttgttcttt
     6541 ttctgataga agatagatcc tttccggtaa aaatacttgt ccatctgccc acaacgtacg
     6601 gacaaattgt aaaaaggatt gcttgaaaat aaaattttct gcatcgtcga tttcagcgat
     6661 actaataatc tgtaaaatca attcattcag tgataggtca ttgagaacaa aatttttata
     6721 aacaacttcg aaatcttttc ttaacagctc catgttctca ttggtataat ttctactgtt
     6781 gaacaaagaa aaaatataat agacatcttc ttcttgtaag tctagaattc ctttttgctc
     6841 ttccaatcct tgacagacta attgaaataa cggcttcgtt tctgcgcttt tcttatcttc
     6901 ttctttcagt ccaaggggaa atgcctttct tccgattgcg atctctattc ctcgtatgat
     6961 aaaggacttt tcatcttccg aatatcttct tttaaaaaaa tgagtctcta cataatcaat
     7021 caactcgtta caagcttctt ctacatacgg atttttattt tcatagcctt cccaattgac
     7081 ataacgtact aaagctagca agatgttccg cgagtcaaat tctgggataa tgatttcttt
     7141 atttttcaaa taatcatttt ctttaaaaaa atccaatacg atttttttga ttgtatatac
     7201 tttgctcaaa gaaataaatt caagatcagc cagttctgta ctcgtgaata taccattaaa
     7261 ataataactt aaaaacttta aaaaataaga ttgaccgtac agaaactgtg tgagctcaga
     7321 aagagcaata ccatgtttga atacgagttt atacttatgc ttctctctta cttgttcttt
     7381 tatacaatat tctagacact cggctagact ttctagatcg tgaaggatcg ttggtgtagt
     7441 cacccttaga gaaatagctg tttgttcgat gtctatttct tggaattcaa ataattgttc
     7501 acacagatac acttttctaa atatatttcg ctcgatgtaa ttttccagca ttcctgatac
     7561 acgcctccag tttttcttat gattttattt aatcatatct gtaaatcgta tttttgtttc
     7621 ctttttattt agaaaagccc tttcaaaaac caataaaaaa agaaactggg acaaaagtca
     7681 aacaagctca caatttcagc ctcattgtag gtagctttga gccgtttgga cttttacccc
     7741 agcctctttc aagaatggtt attcaaagat gtcagcgtct agttctttga ctacatcttt
     7801 caacaattgg ataccagctg cgctgttacc aaatggatct agtgccgggc tgaagacacc
     7861 tatgccgtaa cgatttggca cagctgccat caacccgccg cctacaccgc tttttgcagg
     7921 tacaccaaca tggacggaaa actctcctga ttcatcatac aaaccagcag ttgtcataat
     7981 tgatttgacg attgtagcac tttcttcagt aatgatttgt ttgccattcc aaggagcaat
     8041 tcctttattt gccagcacag cagctaattt tgctatgcct gtagcggtga cattgaggga
     8101 gcattgtctg aaataagcat cgagtatctc cggtacgtct ccttcgatca tttgattacc
     8161 tttcatatag taagcaagag agcgattgat atcacctgtt cttgattcag aatgatagat
     8221 ttcttcattc aatgtgatat taggatcatc acagatctct ttcataaatg acaagattcg
     8281 ctcaaatttt tcatctgcat ctttaccggc aatcaacgag gttgttgcaa tcgctcctgc
     8341 attaacaaaa ggattcattg gatgtttgcg atgattgatt tccatattca tgatagaatt
     8401 gaaggcaaaa ccggttggtt ccgtgttgat tgtttgaaag actttttcta ttccgttgtc
     8461 tttgattgct aataacaata cgggaacttt tgacatgctt tcgatggcaa agcgcacatc
     8521 ggcgtctcct ccctcaatca gttgttttgt atccaaatca taaatcgcca tacctaattg
     8581 tttgggatcc acttcagcta atgctggaat gtaatccgcc acttgtcctg tttgataata
     8641 gtggaaattt tttccaatca attgttctaa ttcttttact tccatttgaa taaccagctt
     8701 tcatttttta tttttctgca tgctggaatt tatgacggca tgcgtagatc gtaaatggaa
     8761 taactaagac gataagataa gaaacaatca gaatcgtttc ataaatccca tgctgtccag
     8821 ctgcgatact agcaggtgga acaaatgaga tacaaaaagc aaaaagtgaa gtcaaaagac
     8881 ctaaacaagc aacgattact tttacaactt ttccgcctgg aacctgatag gctctttcaa
     8941 gactgtcatc tttaaaaacc agcttcaaat agcccgcgaa aaacataaag tacaacatca
     9001 ggtagattac aacagtcaat gacatcgctg caagaaatga aagattattc cccccgccgc
     9061 ttaatgtaaa gacggctgcc cagattgttg caacgatccc atttgcgatc atcaaatgag
     9121 caggaacacc gtgtgtgttt tcttttgcaa aatattcagg aagtaatcct tcttctgctg
     9181 ctacgtgaag tgcttttgaa ggagaggtta cccaagaagc aatttctccg atgacaccta
     9241 aacaaacaag aatcgcaaga attttcgcaa tccatgcaag atttggatca atgtgtctta
     9301 ataggaattc taacgattga atcacccctg tattaagaga caaaccatgt tgaggtactg
     9361 tagtagcgac ggtcaatccg ccaaaagtat ctaaaatgat tgccagaata accaatacaa
     9421 acattgctaa tggataatcc tttttagggt tttccatttc atttgcatga ctggcagatg
     9481 tttctacgcc catatatgct aaaataaagg aaacaaaaac aaccagcgta ggcaatttcg
     9541 aaaaatcagg aatcaatgtt ttcatagaca gtatcgtttg tagtggagcg cctgaagcga
     9601 cataatggat ccctaagcca aacaagacta atgctggcaa caagatacca gctacaaaac
     9661 cgatccgagc aatcaatgaa gtgtatttgg ttccttttaa ctgtgagagt aaaatagccc
     9721 agaaaataat caataggatt gccaatttca gatatttatt gttttgtata gcaccaatac
     9781 ccgttgcata ggataatgca ccagttaaga aatacagcat ggtcaagtag ccaactgtta
     9841 tctcaaacca ttggaaaaag attgcggcaa aaccgaatcg ttttcccaat gtgcgactga
     9901 cccaagtata gacaccgcct ttttcccatc ctttcaccgt tgccatctct gctgcgcaca
     9961 atgccacagg gataaaccat agcaaaccgc ctaataacaa gaaaaatact aagctgaagc
    10021 ctgaggtcgc gaatgtggga tattcatata atgaaacgac cattgagatg gtcatagcca
    10081 aaaagccaaa gaacgttaat ttttttgcag gaattttcgt tttgctttgt tgcatgaatc
    10141 ttcctctttt ctatttgaat ttgtttcaat tgagtttgtt gccaaagaag aaaaagtcca
    10201 atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc gccgatttta
    10261 aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca atcacttgaa
    10321 tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat gcaaagatcc
    10381 ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca tcaagtgcat
    10441 catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata cccgcaatga
    10501 tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta atggctgcca
    10561 atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct tttacttttg
    10621 caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa ccaaatgcat
    10681 tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac cagccaaacc
    10741 aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt ctttctccta
    10801 aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat ccagctgtcg
    10861 tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa aatccgccac
    10921 cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata acaagtataa
    10981 tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga acggtaatca
    11041 cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca ccatgaaaca
    11101 tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt ccaataacac
    11161 cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata atcgttgtta
    11221 tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc cgtgctaatc
    11281 cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta cataaaatca
    11341 tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc tcctttctac
    11401 gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg aattaatact
    11461 tgcatttata aaagacagac aattaataaa agcttgatgt gttgtgctct ttttcgtttt
    11521 tcacacaaat aaagacaaaa aataaacggt cttcttttta tagaaaaccg tttattttcg
    11581 tcttttttta tttttaaaga tattttttca taaggaataa aga
//
LOCUS       DACDTH010000082        11293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDTH010000082 DACDTH010000000
VERSION     DACDTH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.083"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..648
                     /locus_tag="IWK54_002457"
     CDS             1..648
                     /locus_tag="IWK54_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594550.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(741..3020)
                     /locus_tag="IWK54_002458"
     CDS             complement(741..3020)
                     /locus_tag="IWK54_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9594551.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            3298..5121
                     /gene="cadA"
                     /locus_tag="IWK54_002459"
     CDS             3298..5121
                     /gene="cadA"
                     /locus_tag="IWK54_002459"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9594552.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5168..5347
                     /locus_tag="IWK54_002460"
     CDS             5168..5347
                     /locus_tag="IWK54_002460"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9594553.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            5684..7624
                     /gene="thrS"
                     /locus_tag="IWK54_002461"
     CDS             5684..7624
                     /gene="thrS"
                     /locus_tag="IWK54_002461"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9594554.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            7848..8696
                     /locus_tag="IWK54_002462"
     CDS             7848..8696
                     /locus_tag="IWK54_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9594555.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            8990..11143
                     /locus_tag="IWK54_002463"
     CDS             8990..11143
                     /locus_tag="IWK54_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9594556.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         3637 a         2004 c         2406 g         3246 t
ORIGIN      
        1 atggaagcaa aaatcacttt agaacctttt gaacgtatct taagcggtta ccgcaaagta
       61 gaagagttag ctgtgaacgt aactgattgt tcaaaattag cgcaaaaata cgcaagattc
      121 ggcgttgaag gctatcgatt aggaaactat gtcggtacag ggtatctgaa ccgttattta
      181 gagtgtatgg tggaccgcgc accaatgctg atttatagac aaaattatct gattccgctg
      241 ctttttcgtc gttctgattc agcctttcgg ttatttgaag aagagtatcg gatggaggct
      301 ttctttctac tattagagtg gagcttgaaa catcgtcctg aaaaaattct tatcgaaaga
      361 aatgagaaga tagacacgaa aaaaaataaa gtgatcgata gtgcatacct agcatttcgt
      421 gtatcagaga ttttggattg tggcggctat ccaatcagca atttccaatc aattgaccaa
      481 ttcattgaat ggaatcgaat ttaccgattg atcgacaatg gcgggattgg ccgtcatagc
      541 aaggtatttg acccagaata tccagaaaac atggaagaac tgaaaatgat tatttcgctt
      601 gtcaaattaa aatatcctga aacagatttg gatttatata tagaataaat gaccttaaat
      661 gattgaatta ctttttctgc aattattatt gtaaataaaa agacaactgc taacattaat
      721 gatagcagtt gtctttttac ttacagtact tttttctcat aagatgaaat atccatattg
      781 tttaacaaag gactttcatt attggaggta aaaagggtca attcatgcat cgcacgtgta
      841 cacatggtgt aaaggatcaa tttgtcttgc tgagaagaaa aatgttctgc atctacatcc
      901 cacaagaaaa ctcggtcaaa ctccagacct tttgctaaaa aggctggaag taaaataatg
      961 gaacgcttca taaagtcact ctcatttaaa atcagctgga cttgttgttt caactcgtct
     1021 ggcagttgag cataaagagc ttcgcattct tcttgtgttt tagctatgat tgctgtacgc
     1081 caataccggg cttttccctg agtcgttcga atggtatctg ctaaccaatt caatttgata
     1141 tcatctgttg ttccttggat gagcaaagga aggtctcctt ttcttgctgt tgtttctact
     1201 tgatgttctt tcacaataaa atgattagca aaatcagtga tttcttttgt ggagcgatag
     1261 cttgtcgtta attggaaacg agtgacttca cggtccacga aaaggtcttc caatgatcca
     1321 acgatcgttt catttccaaa gacattttga tttagatctc cacagaaagt caaatttgct
     1381 tttgggaaca aggcttgcag caatgccact tgtgatggtg cgaaatcctg catttcatca
     1441 ataaagatat agcgagcttt gtgtgtaacc gtcactggat atgttcggcg catcagtaaa
     1501 aagaatagta cagcatcctc ttgacgcaat tctctttgac gcaagtgtgt acgaacagtt
     1561 gtcaaatgtt cctgccacct ggtctcgctg attttatgtc gtgttagaag agaaagtggc
     1621 acagtttgta gaaagtggag atattgtttc gttatattga caaattgata attcatgatc
     1681 cctcgatgga ttcgacggaa tcttttacgt acgatttggt tggccagttt tttacgtaat
     1741 gaacgctcct tttgttctgt atattcaaga tttggatctg ttgcgtatag ttcttggagc
     1801 tgttcttccg ccagatcttt gacccattgc tgacgtgttt cgtctttttg cagaccgcct
     1861 aattttttca ggagcttcgt ttgtaataga gaaagtctat gatgtaaagg taattgttca
     1921 tttgtttctt tataccattg gcgaatcgat tcctttgata ggatcgtacg gccatttatt
     1981 ttcatatcac ggaacaaggg accgtagttt gtgatgcctt ggatatagcg atcaatctga
     2041 tcgactaagg tcaatccgct tttcatcact tgtatcggat ctttttcccc tagcaaaaaa
     2101 ccactttctt gttgctgttc atctagtaaa gaaaactctg gcagcagctg caccaaataa
     2161 tttttgaatg tctgtgtagg cacgccgctt tcacctaatg aaggcaatac agtcgagatg
     2221 taatcagaaa acaaatggtt gggtgaaaat agaagaacgt tttcagcctc gagccacttg
     2281 cgattatgat acaacagaaa agcaatacgt tgaagcaaag cggatgtctt tccacttccg
     2341 gcgattcctt caatcagcat tactttactt gttgtgtccc gaatgattgc attttgtgct
     2401 ttttgtatcg tagagacgat gtttttcata tggttgcttg atgattcatc caatatctct
     2461 agcaaaaagt catcattgat tacttccgac gtatctacca tagataaaat acgtccttct
     2521 tggattttaa attgtcgttt caactgcaga tcgacatcta ttctatctgt ttcagtttca
     2581 taaaatgcgg gaccaatctc accttcataa taaagattgg caattggtgc gcgccagtca
     2641 ataacgattg tttcttctgc cagatctctt aaagaagcga tacctagata taatgtttct
     2701 gtttcacggt cttctttaaa gtcgatacgg gcaaaataag gactatcttt catgacagac
     2761 aacgtttgca ggcgtttttg ctgtgcttca agagtatggt atttcaaaag caattcctgc
     2821 tcatgttggc gatattcaac agcagattca taaaaagctt cattggaacc agctcgaatc
     2881 ttgtgattag ctgtgtcttt tgtcgaatga ttcatttcct gagtcaattc ttgttgtttt
     2941 gtatccagca tattttgttc gatctggatt agttcgatcg tttcatccac gtgcgtttgt
     3001 tctagttgtt tctcattcat cgcatgtttg ctccttccgc cgtaaataac gaattaaaag
     3061 tatagcatag tttttcgaaa aaaggtactt tttaactgat gaatcgaaaa agtaatcctt
     3121 gctgaatatt tttgtttgta gaactgatga attttggaaa cgctgatagt gatagttgtt
     3181 ttcaattaag gcctattgtt ttaaaaagtg ctagtcaagg actatgctta agatgaaaga
     3241 ggcagctgga aatttattac taacctctct aagtcacata aaagaggaga aaaaacaatg
     3301 acacatttca aaaaattttt acttactttg cttattggtg ggatagcttt attaagtgaa
     3361 tttgtttttc accagccggc ccttgcattt gcgattgtcg catttactgg agggattctc
     3421 gccttcttga tgtttttgga aatgatcaag acgttacgtt caggaagata tggcgtagat
     3481 gtattagcga ttaccgcaat ccttgcaaca ttagccgtca aagaatactg ggctagtttg
     3541 atgatattga tcatgcttac gggcggagat agtctagagg attacgcgaa taaacgagcg
     3601 ggtaaagaac tgcagtcttt gttagataac accccaagaa ttgcccatcg attcaaagaa
     3661 ggaaaattgc aggatatagt aatcgatgaa ataaatattg atgaccagtt actagtcaag
     3721 cctggcgaac ttgtacccgc agatggtcga gtgaccaaag gagaatcttc ggtcgatgaa
     3781 tcttctttga caggtgagtc ccgcccggaa accaagaaac ctggagatca gattatgtca
     3841 gggtctttaa atggagacgg atcgatccaa atgatcgctg aaaaacgtgc cgcagacagc
     3901 cagtatcaaa tgattattaa actagtcgaa gaatcaaaag aaaagcctgc ccgatttgtc
     3961 cgtttagctg atcgttatgc agtccctttc acgttgattg cttatttgat cggtggtatt
     4021 tcttggtggt tctcaaaaga ccctttacga tttgctgagg tactggtagt ggcatcacct
     4081 tgtccactga ttctagctgc gcccattgct ttagttgcag gaatgagccg ttcaagtaaa
     4141 aacgggatcg ttgtaaaaac tggaacaacg attgaaaagc tcgcacgtac aaaaacgatt
     4201 gcttttgata aaacaggaac gcttaccaaa gggatattgg aagtagaggc gattcagcca
     4261 gtcaatggaa taaaaaaaga agagctactt ctgtatgcag ctagtgcaga acaggaatct
     4321 agccatatct tagctcgttc gttagtgaac aacgtgaaaa acagccaact gtatcctgtt
     4381 agtcaactga aagaagtgac cggtcaagga atccaagcag tagtcaataa tcatgaagta
     4441 aagattggac gtgcctcttt tgtttcagct gaagaaatag aaacgtccaa tacagcaatc
     4501 tatgtaacaa ttgatcgatc ttttgcagga acgatcctat tttcggatca gattcgccca
     4561 gaagcagcaa agacaatcaa ggagctaaga aaacttgatg tgaaaaattt gatgatgatc
     4621 accggggatg gatctgccat tgctcaaacg attgcagaaa aagtccaatt agattctgta
     4681 catgcccgta gcttgcctca agataaactg accattttag aaagtatccc taaagatcaa
     4741 agacctgttg cgatggtagg agacggcgtg aatgacgcac cagcacttgc tgctgcagat
     4801 atcggtattg caatgggggc tcatggttct acagctgcta gtgaaagtgc agatgcggtc
     4861 atcttgaaag atgatctctt gcgtgtagca aaagcagtag tgatttcaag agatacgatg
     4921 agaatagcga aacagtctgt actgattggc atctttattt gtattttttt gatgttaatt
     4981 gcaagtacgg gactaatccc cgcattattc ggagctgttt tgcaagaagt cgtggatacg
     5041 gtctctattt tgagtgcctt gagagcaaaa aaagatccag ccatttttcc tgattcagta
     5101 cccaagacaa acatagtatg atagaatcga gttgaaaaaa gaacgatcag aaagaggaga
     5161 gagcattatg ccattcgtac atgtcgaatt agtagaaggt cgcagtccgg aacaattaga
     5221 aaatatgatg aaagatatca cggaagctgt tcataaaaac acgcaggcac caaaagaaca
     5281 catccatgtc atcatcaatg agatgaaaaa aggaacatac ggtgtaaatg gggaatggaa
     5341 aaaataagtt tagtttccca cttgattttt ctatttatta gtggttaaat aaacacataa
     5401 ttgattgatg agaaagccaa ctgaatttga gagtcttcga taagagagga aaagctaggc
     5461 tgaaactttt ccaggagaaa accaaaggat gaccactttc gaaacccttg ttgaagcaag
     5521 ggcggtcgga ccgttacaac gcttgaggaa cgcatttgcg tttgaacatt aggtggaacc
     5581 acgaactttt cgtcctagta tctcttttca agagatgcta ggattttttt gtttttcagc
     5641 ttgatgatct gatcaatcaa aacatttcaa ggaggaaact attatgataa aaattacttt
     5701 tccagatggt gcagtcaaag aatttgagtc tggaacaaca acattggcaa ttgctgaaag
     5761 catttcaaaa agcttagcaa aaaaagcttt agctggtaaa gtgaacggaa aactcgttga
     5821 tttgactcgt ccaatcgagg aagatgcaag tattgagatc atcacaccag accatgaaga
     5881 tgcacttggc ttagtccgtc attcagcagc tcatttgatg gcccaagcca tgcgtcgcct
     5941 ctatccaaat atccactttg gcgttggacc agctatcgat tcagggtttt actacgatac
     6001 agataatgga caaaaccaag taactgcaga agatttgccg gcaatcgaag cagaaatgat
     6061 gaaaatcgtc aaagaaaatc tgccgatcga acgccgtgtt ctttcaaaac aggaagcatt
     6121 agaaattttt gcaagtgatc cgtacaaagt agaactgatt agtgaattgc cagaagaaga
     6181 agtgattact gcttatcaac aaggcgaatt catcgatttg tgtcgtggac cacatgttcc
     6241 atccactggc cgtatacaag tatttaaatt attatcagtg gcaggtgctt attggcgtgg
     6301 aaactcaaac aatcaaatga tgcaacgtgt atacgggaca gccttttttg acaagaaagc
     6361 tttgaaagaa tatatccgtc tccgtgaaga agcaaaagaa agagaccatc gtaaactagg
     6421 aaaagaactt gacttgttca tggtttcccc agaagtaggt tctggtttgc cattctggct
     6481 tccaaaaggg gcaacaatcc gacgcacgat cgaacgttat atcgtagaca aagaagttag
     6541 tctaggttac caacatgtat atacaccaat aatgggagat gttgagttgt acaaaacatc
     6601 tggacactgg gatcattatc aagaagacat gttcccgcca atggatatgg gagatggcga
     6661 aatgcttgtt cttcgcccaa tgaactgccc tcatcacatg atggtctaca aaaacacgat
     6721 ccattcttat cgtgaattac caattcgtat cgctgaatta ggaatgatgc atcgctatga
     6781 aaaatcaggt gctttgtcag gtttacaacg tgtacgagaa atgacgttga atgatggtca
     6841 tacatttgtt cgtcctgatc aaatcaaaga tgaatttaaa cgtacgcttg aattgatggt
     6901 ggcagtatat gctgacttca atattacaga ttatcgtttc cgcttgagct atcgcgatcc
     6961 aaacaataca gataaatatt ttgatgatga cgcaatgtgg gaaaaagccc aagcaatgct
     7021 gaaagccgct atggatgaat tagaactaga ttactttgaa gcagaaggcg aggcagcctt
     7081 ttacggacca aaactagatg ttcaagtcaa aacagctctt ggtacagaag aaacattatc
     7141 aactatacaa ttagacttct tattgccaga gcgctttgat ttgacatatg ttggagaaga
     7201 cggggaaaat acgcatcgtc cagtagttat ccaccgcgga attgtttcta caatggaacg
     7261 ttttgtcgct tatttaacag aagtatacaa aggcgctttt ccaacatggt tggcacctat
     7321 acaagcaaca atcatccctg tttctgttga tgcacatggc gattatgctt atgaaatcaa
     7381 agaacgtttg caaatgaagg ggttgcgtgt agaagttgat gatcgtaatg aaaaaatggg
     7441 ctataagata cgtgcctctc aaacacaaaa gatcccttac caattagttg ttggagacaa
     7501 ggaattggaa gatgcaacag tgaacgtacg tcgttatgga agtaaagaaa cagctgtaga
     7561 agatttgaat atctttattg atgctatgga agcagaagtc aaaaattaca gcagagaaaa
     7621 ctaataggta aatttcctgt aaaaaagtag tagatagaaa gcaatccgaa caatagataa
     7681 caggtattcc tcaaagacac agatgctcaa tagaatctgt gggattgatg ggataagata
     7741 ttctgttggg cggattgttt ttttgttttt tatccagtat acgaccgtga ataagggtct
     7801 gatatggtat acttacatag actatcatta aaagggtgga ggaacttatg agcgtaatta
     7861 gtcgtattga aagtatgatg gaagattatt cttcagcaga aaaaaagttg gccaactaca
     7921 ttatcggtaa tattgagaag gtccctacaa tgacagctaa tgaattagca gaagcttccg
     7981 ggttgagtgc gccgacagtt gtacgttttt ccaaaaaaat cggttttcaa agtctgacgg
     8041 acttcaaaat cacgatttca acagaactac agactggaat cgatgaaggt tttagtgata
     8101 tagaagcaaa tgagtccttt tactctatca aaaacaaact tggtaacaat gcacaagtag
     8161 cgataaaaga aacagtagat attctagaag aagaaacgat ccaaaaagtc gtagagtgtt
     8221 tggaatcggc agagactgtt tttttatatg gggttggcgc ctcttcttta gtagtagaag
     8281 atattctgca aaaatggtct cgtgtcggta agccaatcat ttttgaaaaa gatatccacg
     8341 tcttgctccc gcaacttgtc agcaatgaga aaaagaaagt gctttggctt gtttcaaact
     8401 caggtagaag tgcagatgta gtcgcattag ccgagctggc gaaaagcatg aatatcgaaa
     8461 tcattgcgtt gacgcaattt ggaaataacc cgcttagcaa gatagccgat gtactcgtac
     8521 aaacatctag accaaaagaa atcacgaatc gaagtgcggc aactaattca cttttggccc
     8581 aatttgctac gatcgatatc attttttatt tctatatggc taaaaacgaa aagcttagcg
     8641 aaaaagtgaa aaaaacacga gaagcaatcc aagaatattt tatggattcg aaataaaaac
     8701 cttactattt tttatataag ttttttagaa taacaaaacg tagttttcca caaataaatg
     8761 atccatcaaa gtgtttcacg acttaagaaa acataatttt caaaaataag tttatttttt
     8821 aatgaaaaaa atagttggat atgtaacttt aagtgattgc tgtgaaacaa aaagttgatt
     8881 ttatcttgaa attaggtaaa gaatttcttg ctccgcccat tttttctttt ttattaagaa
     8941 gtttttgggt acaatgaagc atgaatgtaa ataggcggaa ggataggaaa tgatgaaaga
     9001 ttttatgaaa aaaatattaa atacttcttg gttagaaaaa ataagaaaag gtgcacaaga
     9061 acctaagaac aaaacgtttc gccggtccca tgttcctttt cgcttaaatt ttcttttttt
     9121 cattatattt acgttatttg ttgcgttgat tgctagattg ggttatttgc agatcgtgaa
     9181 cggcgaagag atggaggcgc gagtgaagtc tacttctacg attacggtcc aagaaagtac
     9241 tcctcgcgga atgatctacg actccacagg aaaagcttta gttacgaata aagcgaatca
     9301 ggccatcaca tatacgagag gaacacaagt tacggctaaa gatttgcttg aaatcgcaac
     9361 gaagctgaac tcgctgatcg atgttccggt agatgaaaat ctgacagata gagataaaaa
     9421 agatttttgg ctggctgatc ccaaacattt gaaagaagcc acaaaaagac tttcggcaaa
     9481 agaaaagcaa ttagggaaca gcgaacaata tagtgcgatg gtcaataaag tcacagctga
     9541 tgagattaat ttaaacgagg agcaattaaa aattgctacc atctttaaac ggatgaatag
     9601 tgcatatgaa ttaaacactg tctatatcaa aaactctggt gttactgatc aagagttagc
     9661 tgttgtggca gaacgcgctt ctgaacttcc aggagtttcg actggcacag actggacacg
     9721 agaatataat gcagcaagtt cactgaaaag tattcttggc agtgtgacga ctgaaaagca
     9781 aggacttcca gcagatgaag cagagaaata cctagcaaaa ggttattctc ggaatgaccg
     9841 tgtaggacaa agttatttgg aaaaacaata tgaagatgtc ctgcaaggaa ccaaaaccca
     9901 atatgaagtt tcccttgata atgaaggaaa tgtttcgaat caaaaagaaa tcttctctgg
     9961 ggaaaaaggt tctaatttaa tgttatctat gaatgcagaa ttccaatcaa aagtagaaga
    10021 aattttgaaa aggaattatc aaacattgat caacaacggc aaagcacagt attcgcctgg
    10081 tgcttatgca gtggctatga atcctcagac aggagaagtg ctagcaatga ctggtttcag
    10141 tcatgaacaa ggttccaaag aaatcaccga gaatgcttta ggcacgatta ctagcgcctt
    10201 tgatccaggg tctgtagtca aggctggtac tttgacagca ggctgggcaa gtaacgcgat
    10261 cagcggaaat caagttttga ttgatgagcc gattcgtctg caaggtgctt cagaaaaaag
    10321 ctctgtcttt aatcgaagcg gacaagttgc tcttgatgca gtcaaagcat cggaattgtc
    10381 ttctaatacg tatatgatca aagtggcgtt aaagatgtta ggactagact atacacctgg
    10441 aatgggacta cctagtttag atgaagaagc taaagcttat cagcaattga gagactcttt
    10501 caaagagttt ggtcttggta caacgactgg gatcgatttg ccgaatgaat ctcctgggat
    10561 ctcacgttct gttgattata tgaagaagtt caatgcggat aatggcaaag aatggtatgc
    10621 tccaggtaac tttaccgatt tggcatttgg acagtttgac acatatacac caatccaatt
    10681 agctcaatat gcttcaacag tcgctaatgg cgggaaacga gtacagccgc acctagtcaa
    10741 agcgatctat ggcaatgacg aaaatggaaa cttgggtgaa gtgaagaaag aaattggcac
    10801 aacagtagaa aatacggtca atatttctgc agaaaatatg gcgatcctcc gtgaaggatt
    10861 ccaccaagtt gtccatggta ccgatcctta cacaacagcc aaaccattag ccagtgcgaa
    10921 aatggacctt tccgcaaaaa cagggactgc agaaacggtt gcggaaggtc atccagatat
    10981 tgcgacagtc aatagtaata tcgtggcata cggaccaaca gacaatccgg aaattgccat
    11041 cagtgttgtc ttgccgaact tattagacga acaagaccac ataaacttga cgattgctaa
    11101 agaaataatg gacgcgtatt atgatatgtt catggcaaaa taaaaaagag gtcgtgaaaa
    11161 agccgcttag tttcgagtat gaagaaaaat tttgtgaaaa tagcttctca catttttgct
    11221 taaatttgac ttcataccga gaaactggct tttgaacacc gtttactcgg caaaaaaggt
    11281 tgtgacaaaa att
//
LOCUS       DACDTH010000083        10929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDTH010000083 DACDTH010000000
VERSION     DACDTH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.084"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..288)
                     /locus_tag="IWK54_002464"
     CDS             complement(<1..288)
                     /locus_tag="IWK54_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9594557.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAEN"
     gene            430..1548
                     /locus_tag="IWK54_002465"
     CDS             430..1548
                     /locus_tag="IWK54_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9594558.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1676..3052
                     /locus_tag="IWK54_002466"
     CDS             1676..3052
                     /locus_tag="IWK54_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9594559.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3453..5033
                     /locus_tag="IWK54_002467"
     CDS             3453..5033
                     /locus_tag="IWK54_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9594560.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5231..5454
                     /locus_tag="IWK54_002468"
                     /pseudo
     CDS             5231..5454
                     /locus_tag="IWK54_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5659..5979)
                     /locus_tag="IWK54_002469"
     CDS             complement(5659..5979)
                     /locus_tag="IWK54_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9594561.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6127..8361)
                     /locus_tag="IWK54_002470"
     CDS             complement(6127..8361)
                     /locus_tag="IWK54_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9594562.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8840..9106
                     /locus_tag="IWK54_002471"
     CDS             8840..9106
                     /locus_tag="IWK54_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9594563.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9106..10833
                     /gene="ptsP"
                     /locus_tag="IWK54_002472"
     CDS             9106..10833
                     /gene="ptsP"
                     /locus_tag="IWK54_002472"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9594564.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1912 c         2216 g         3322 t
ORIGIN      
        1 gttttctgct tcttcaatca atatttttcc taacccttgg tgtctgtatt cagaaagaat
       61 cgccatccgc tgtaatttca tttttccgtt ttctaaaggc aaaagacgta cagttccttg
      121 cggttctttt ttatctgagt acaggacgaa gtggatacaa tgggcttcat ctttgtctat
      181 ttctcgagac aaaggcacac cctgttcgac tacaaaaact tgatgcctga tccgtacagc
      241 atctaagtaa atattgctca ttgtatcttt tgtttgtaca actttcatgg tctagccacc
      301 ttcctttcgt atagtatact ggaaaataaa acaaataaaa agtatggcaa gttttcaaaa
      361 ataaaaaaag aataaaagaa tttttaacga atataatata tactagtaag aaagaaagga
      421 ggaaatctat tgtttgaaaa acttagacag tctaaactaa tgttttggtc gcttgaatta
      481 ttgatcatag ctgcattgat catgatttca tcagaaataa actttatttt cgaaccgatc
      541 gggacatttt tttccacgtt atttgcacct gtactgattg caggctttct ttactattta
      601 ttgaatccta ttgtcaagct gttgatgaaa gcaaaaatca aaagaatatg ggctgtcgca
      661 attgtgctgc tccttttagt tgcagcaatt atttggattt tacttagtgt catcccaagt
      721 ctagtacagc aaatttcctc attggcatct aatatgcctg atttcatcaa acaggtagaa
      781 agctggttaa aagaggtagc cgaattgcct ttgttcaagg aagtagatat caataaatat
      841 tttgagcaat tagacatttc ttacgggaca atcattcagc aattccttag tggagtatcc
      901 aatagtttag gttcgatcgt tggaacgatc gcttcggcaa ccgtagtaat cattacagca
      961 ccattcatct tgttctatat gttgaaagac ggagaaaaac tagtgccaaa catccaacag
     1021 tttttcccag aaaaaagaag aaaccaaatt gtggagttat tggggcaact gaataaaaca
     1081 ttggctaatt acattagcgg acaagcaatc gaatgtttat tcgttggtac cttcacattt
     1141 ttaggatata tggtaattgg tgtggattat gctttcttat ttggggtcat tgcgggattg
     1201 acaaacctaa ttccatattt aggaccttat ctaggactag cacctgcatt tctagtaaca
     1261 gtcttcaatg atccggtaaa agcattactt tgttgtgtgg ttgtgctaat cgtccaacag
     1321 ttggatggga atatcattta tcctaatgta attgggaaat cactgaaaat ccatccatta
     1381 acgatcatta tcgttcttct agtagctgga aatattgccg gattgcttgg tatcttctta
     1441 ggtgttcctt tttacgcaat ctgcaagacg atcatttcct atataatcaa aatcgtcaaa
     1501 gaagacaagc aaaatgaaaa caagaagaaa attgctacga cctcgtaggt agttttagta
     1561 cacttgtttt tattgcaaag aaaattgctt tgtgatacca ttttaacgtg gctatagtgc
     1621 cacgtttttt tctatcgtaa actaaaaatt aattttataa ggagagaatg cgattatgaa
     1681 tgctgaccct gagagtcagt cgctgttagc gcagatttta ttattgatcg ttttgacatt
     1741 gatcaatgct tttttagcag cttccgaaat tgctgttgtt tcgatcaata agaatcgaat
     1801 cgagcaaaaa gctgatgaag gagatgtgaa atctcaaaag cttttaaaaa tattgcagaa
     1861 tccaaataat ttcctatcga caatccaagt aggaatcact ttggtcaaca ttttgtctgg
     1921 tgcttcacta gctaatactt tatcggaacg gttagctccg gtattaggtg gcggcacagc
     1981 ggcaaaaaat attgcaaata tcattgtgct ggctattttg acctatgtat cgattgtttt
     2041 tggagaattg taccctaaac gtattgcttt aaataaatca gaagaagtcg caaagttcac
     2101 ttcagggctg atacgcttga ttggtgtagt agctaagccc ttcgtctggc tgctttctgc
     2161 ttccacaagt ttattggcgc gtatcacgcc gatgactttt gatgatgaag attcaaaaat
     2221 gaccagagat gaaatgcgtt atatgcttga aaacgaaggc gtattgaata atgaagaatt
     2281 agaaatgctc caaggggttt tttctcttga tacgaaagtt gcccgagaag tcatggtccc
     2341 acgaacggat gcatttatga ttgatatcaa cgacactgtg gaagaaaatg tgaatgaagt
     2401 tttatccgaa aactattcaa gaatccctgt ttataacgaa gacaaagata aagtagtcgg
     2461 aatccttcat acaaagaatt tactaaaagc tgcccataaa ttcggctttg ataatttaga
     2521 tatcaagaaa atcatgcagg agcctttatt cgttcctgaa acggtcttta ttgatgatct
     2581 gttatatgaa atgaaaaaga cgcagaatca gatggctatt ttattagatg aatatggcgg
     2641 cgtcgttggg ttagcaacac tagaagacct tttagaagaa atcgtcggag aaatcgatga
     2701 tgaatcagat gaagttgaga atctttatga aaaaatcgat gaacacgaat atatcatcca
     2761 aggacgtatg ctgattgatg agttcaatga agcttttgac agtgatctgc atatgagtga
     2821 tgtagatacg atggctggtt atttgattac agcattaggc atgattccgg atgaaggcga
     2881 aaagttatcg tttgatgtcg ataatattac gctggtttct gaagaaatgg aaggttctcg
     2941 aatccttaaa atccgtgtca tttttcatga tccggaagaa acagaagcgg aacctgaaga
     3001 agaacgaaga tacttcagaa aagaattcga agatgacgag ccaagaagat aattggaaac
     3061 ttggttatga aagacgtgtt gcttgaataa acaagcaaca cgtcttttct tttagtcagc
     3121 tgacaaaaaa atatttgact cttatggttt ggtggctttt tcattcgctg aatccatgct
     3181 atactgattc agttgtagta gttgacaaag gttgtgaaaa agctatcctg cattaagtaa
     3241 taagagcagc caagcaaaaa cagcttttcg tatttttggc ttgggtgggc ttattgctga
     3301 taggctgtct ctagaacgcc gtttactcgg aaaaagagac tgctacacgg cttttgtcac
     3361 agccctatat gaaattgtct ttctttaaga gatcggacaa tctgtgccta gagaaaatat
     3421 aaataattga atggaagaaa tggagttagg ttatgaataa tccaaaatta aagcaacaag
     3481 tggacaatcg cagaactttt gcgatcattt cccatccgga tgctgggaaa acaacgatta
     3541 cagaacaatt actattgttt ggtggggcta tccgtcaagc cgggaccgta aaaggcaaaa
     3601 aaacaggtaa ttttgccaaa tcagactgga tggaaatcga gaaacaacgt gggatttctg
     3661 ttactagttc tgttatgcag tttgattatc aagggaaacg agtaaatatc ttggatacac
     3721 caggacatga agatttttct gaagatactt atcgtacttt gatggccgtg gatagtgcgg
     3781 tcatggtaat cgatagtgca aaagggatcg aagcacagac aaagaaatta ttccaagttg
     3841 taaaaaaacg tggcatcccg atatttactt ttatcaacaa actagatcgt gatggtcgtg
     3901 aaccattgga attgttagaa gaattagaag agctattaga tatcgaatct tatccgatga
     3961 attggccaat cggtatgggt aaagggctag aaggtcttta tgatatctac cataatcgtg
     4021 tagagtttta tcgtccggaa aattatcagg atgaacgatt agtagaattg gatgaagatg
     4081 gactgcttcc cgaaaatcat cctctaactg aaaattcctt atatgaacaa gtcttagaag
     4141 aagtagaact aatcaaagag gctggagatg catttaatca agaaaaaatt gctcgtggcg
     4201 atcagacacc agtatttttt ggttctgcat tgactaactt cggtgtacag acttttttag
     4261 agactttcgt tgaattggca ccagctcctt atggacacaa aacacaagat gatcagatgg
     4321 taagtcctta tgaggaagaa ttttcaggtt tcgtattcaa gatccaagca aatatgaatc
     4381 cagcccatcg tgaccgaatc gcttttgtcc gaatctgttc tggaacattc gaacgaggaa
     4441 tggatgtctg gttggaacgt acaaataaaa aactgaaatt aagcaatgtc acacaattta
     4501 tggcagattc gagagaaaac gtcgaaaaag cagtagcagg cgatatcatc ggtgtatatg
     4561 atacaggcaa ttatcagatt ggggatacat tgttcgaagg aaaactaaaa gttgcttatg
     4621 aagaactccc ttcatttact ccagagctgt ttatgaaagt gactgcaaaa aatgtcatga
     4681 aacaaaaatc cttccacaaa ggaatctatc aattagttca agaaggagcg attcagcttt
     4741 ataaaacata tctaacggaa gaatatatca ttggagctgt tggtcagcta caattcgaag
     4801 tattccaata tcgtatgtta aatgagtata acgccgaagt aatcatgtct ccaatgggac
     4861 acaaaatcgc tcgctggatc gatccagaag atttggatga gaagatgagt tcaagtcgca
     4921 atatcttggc tcgtgaccgt ttcgaccaac cgttgttctt attcgagaac caatttgcag
     4981 aacgctggtt tgcggataaa tatcccaatg taaaattaaa aagtttgatg taaagaagtc
     5041 ttgacacaag ctgccctgca tcaagtaata agaatagcca aacaaaaaca gcctctcata
     5101 ttttgtttag agccgtgacg gattttccgt gacggctctt tttactatat taaaatcata
     5161 gaaatgtcta atcaaaaaaa tataaaaatt attttgcgta catatgatta aagaaaggag
     5221 agtgtgacac atggaaacag aatggcaaag acgtgaaaga aaaaaacaga aaaagaagag
     5281 atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga gcaaattgca
     5341 caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga cacgcgtaga
     5401 actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag ctagaaagaa
     5461 aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca caaaaatttg
     5521 aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc tttcgccgtt
     5581 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttaatt
     5641 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
     5701 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
     5761 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
     5821 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
     5881 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
     5941 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
     6001 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
     6061 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
     6121 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
     6181 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
     6241 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
     6301 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
     6361 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
     6421 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
     6481 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
     6541 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
     6601 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     6661 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     6721 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     6781 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     6841 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     6901 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     6961 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     7021 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     7081 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     7141 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     7201 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     7261 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     7321 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     7381 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     7441 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     7501 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     7561 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     7621 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     7681 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     7741 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     7801 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     7861 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     7921 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     7981 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     8041 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     8101 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     8161 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     8221 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     8281 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     8341 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     8401 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     8461 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     8521 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     8581 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     8641 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     8701 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     8761 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     8821 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     8881 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     8941 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     9001 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     9061 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     9121 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     9181 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     9241 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     9301 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     9361 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     9421 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     9481 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     9541 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     9601 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     9661 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     9721 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     9781 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     9841 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     9901 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     9961 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
    10021 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
    10081 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
    10141 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
    10201 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
    10261 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
    10321 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
    10381 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
    10441 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
    10501 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
    10561 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
    10621 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
    10681 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
    10741 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
    10801 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
    10861 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
    10921 tagacaaaa
//
LOCUS       DACDTH010000084        10790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDTH010000084 DACDTH010000000
VERSION     DACDTH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.085"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IWK54_002473"
     CDS             complement(25..144)
                     /locus_tag="IWK54_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9594565.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IWK54_002474"
     CDS             complement(159..1343)
                     /locus_tag="IWK54_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9594566.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IWK54_002475"
     CDS             complement(1599..1853)
                     /locus_tag="IWK54_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594567.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IWK54_002476"
     CDS             complement(1878..3020)
                     /locus_tag="IWK54_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP9594568.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IWK54_002477"
     CDS             complement(3080..4432)
                     /locus_tag="IWK54_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9594569.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IWK54_002478"
     CDS             complement(4503..4847)
                     /locus_tag="IWK54_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9594570.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IWK54_002479"
     CDS             complement(4857..5231)
                     /locus_tag="IWK54_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9594571.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IWK54_002480"
     CDS             complement(5244..5558)
                     /locus_tag="IWK54_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9594572.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWK54_002481"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWK54_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="IWK54_002482"
     CDS             complement(8976..9638)
                     /locus_tag="IWK54_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594573.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="IWK54_002483"
     CDS             10255..10485
                     /locus_tag="IWK54_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594574.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="IWK54_002484"
     CDS             10491..10790
                     /locus_tag="IWK54_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594575.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         2984 a         2382 c         1832 g         3592 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa
//
LOCUS       DACDTH010000085        10192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDTH010000085 DACDTH010000000
VERSION     DACDTH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.086"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            120..626
                     /locus_tag="IWK54_002485"
     CDS             120..626
                     /locus_tag="IWK54_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9594576.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="IWK54_002486"
     CDS             643..1542
                     /locus_tag="IWK54_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9594577.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="IWK54_002487"
     CDS             1629..2513
                     /locus_tag="IWK54_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9594578.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="IWK54_002488"
     tRNA            complement(2705..2776)
                     /locus_tag="IWK54_002488"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="IWK54_002489"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="IWK54_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9594579.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4373..5410
                     /locus_tag="IWK54_002490"
     CDS             4373..5410
                     /locus_tag="IWK54_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9594580.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="IWK54_002491"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="IWK54_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9594581.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /locus_tag="IWK54_002492"
     CDS             6562..7752
                     /locus_tag="IWK54_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9594582.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /locus_tag="IWK54_002493"
     CDS             7856..8611
                     /locus_tag="IWK54_002493"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9594583.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="IWK54_002494"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="IWK54_002494"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9594584.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2111 g         2630 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct ca
//
LOCUS       DACDTH010000086        10111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDTH010000086 DACDTH010000000
VERSION     DACDTH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.087"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..77
                     /locus_tag="IWK54_002495"
     tRNA            2..77
                     /locus_tag="IWK54_002495"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            79..149
                     /locus_tag="IWK54_002496"
     tRNA            79..149
                     /locus_tag="IWK54_002496"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Gly,seq:tcc)
     gene            163..236
                     /locus_tag="IWK54_002497"
     tRNA            163..236
                     /locus_tag="IWK54_002497"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:197..199,aa:Ile,seq:gat)
     gene            261..348
                     /locus_tag="IWK54_002498"
     tRNA            261..348
                     /locus_tag="IWK54_002498"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:295..297,aa:Ser,seq:gct)
     gene            370..441
                     /locus_tag="IWK54_002499"
     tRNA            370..441
                     /locus_tag="IWK54_002499"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:403..405,aa:Glu,seq:ttc)
     gene            464..553
                     /locus_tag="IWK54_002500"
     tRNA            464..553
                     /locus_tag="IWK54_002500"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:500..502,aa:Ser,seq:tga)
     gene            566..639
                     /locus_tag="IWK54_002501"
     tRNA            566..639
                     /locus_tag="IWK54_002501"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:600..602,aa:Met,seq:cat)
     gene            658..730
                     /locus_tag="IWK54_002502"
     tRNA            658..730
                     /locus_tag="IWK54_002502"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:691..693,aa:Asp,seq:gtc)
     gene            734..806
                     /locus_tag="IWK54_002503"
     tRNA            734..806
                     /locus_tag="IWK54_002503"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:767..769,aa:Phe,seq:gaa)
     gene            811..881
                     /locus_tag="IWK54_002504"
     tRNA            811..881
                     /locus_tag="IWK54_002504"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Gly,seq:tcc)
     gene            894..967
                     /locus_tag="IWK54_002505"
     tRNA            894..967
                     /locus_tag="IWK54_002505"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:928..930,aa:Ile,seq:gat)
     gene            979..1050
                     /locus_tag="IWK54_002506"
     tRNA            979..1050
                     /locus_tag="IWK54_002506"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1012..1014,aa:Glu,seq:ttc)
     gene            1316..2509
                     /locus_tag="IWK54_002507"
     CDS             1316..2509
                     /locus_tag="IWK54_002507"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9594585.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            2639..4111
                     /locus_tag="IWK54_002508"
     CDS             2639..4111
                     /locus_tag="IWK54_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9594586.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4194..4982
                     /locus_tag="IWK54_002509"
     CDS             4194..4982
                     /locus_tag="IWK54_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9594587.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5049..5717)
                     /locus_tag="IWK54_002510"
     CDS             complement(5049..5717)
                     /locus_tag="IWK54_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9594588.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5832..7544
                     /locus_tag="IWK54_002511"
     CDS             5832..7544
                     /locus_tag="IWK54_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9594589.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            7547..9319
                     /locus_tag="IWK54_002512"
     CDS             7547..9319
                     /locus_tag="IWK54_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9594590.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(9401..>10111)
                     /locus_tag="IWK54_002513"
     CDS             complement(9401..>10111)
                     /locus_tag="IWK54_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9594591.1"
                     /translation="TGMIFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSK
                     EEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYE
                     LKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAY
                     NQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTH
                     TTKNMQNQLVEKLGKIEI"
BASE COUNT         2984 a         1741 c         2223 g         3163 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgccatgc gggtgtagtt tagtggtaaa accacagcct tccaagctgt
      121 tgtcgcgagt tcgattctcg tcacccgctt ttagtttttt atgggcctat agctcagctg
      181 gttagagcgc acgcctgata agcgtgaggt cgatggttcg agtccattta ggcccattag
      241 tacaaaatct tattggtttt ggggaagtac tcaagtggct gaagaggcgc ccctgctaag
      301 ggtgtaggtc gggaaaccgg cgcgagggtt caaatccctc cttctccgtt tcaatatttt
      361 gttttacctg gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg
      421 gttcgaatcc cgtacgggtc attaatttaa tatagtttta catggaggat tacccaagtc
      481 cggctgaagg gaacggtctt gaaaaccgtc aggcgggtaa aaccgtgcaa gggttcgaat
      541 cccttatcct cctttttcat attatcgcgg gatggagcag tctggtagct cgtcgggctc
      601 ataacccgaa ggtcgtaggt tcaaatcctg ctcccgcaat taaaaactaa aaacattggt
      661 tccgtggtgt aggggttaac atgcctgcct gtcacgcagg agatcgcggg ttcgattccc
      721 gtcggaaccg tttggctcgg tagctcagtt ggtagagcaa tggattgaag ctccatgtgt
      781 cggcagttcg attctgtccc gcgccattat gcgggtgtag tttagtggta aaaccacagc
      841 cttccaagct gttgtcgcga gttcgattct cgtcacccgc ttttagtttt tatgggccta
      901 tagctcagct ggttagagcg cacgcctgat aagcgtgagg tcgatggttc gagtccattt
      961 aggcccattt attttattgg cccgttggtc aagcggttaa gacaccgccc tttcacggcg
     1021 gtaacacggg ttcgaatccc gtacgggtca tggaagatac tttttgagta tctttttctt
     1081 tttatctgaa agaatgattt taaaaagctt agcagagtta tctttatgga tgattgtgct
     1141 aagctttttt ggacagttct aatgtaaaat atttttatat tttttaagca aaaaaatgaa
     1201 aaaatacgag cagaaaactg ttttatctgc tatacttggt tcgttgcaag ttcccgaaag
     1261 gatccgcagg acggagatgc cttgtaaccg aatttaacta gggggaatca ttataatggt
     1321 agaaagacac ttatttacat cagaatctgt ttctgaagga catccagata aaattgctga
     1381 ccaaatcagt gatgcaatct tggatgcaat tttaaaacaa gatccaacag cacgagtagc
     1441 gtgtgaaacg tctgtaacaa ctggtcttgt tttagtattt ggagaaatat ctacgacggc
     1501 atatgtagat attcaaaaag tcgtacggga aacaataaaa gaaattggtt atacacgtgc
     1561 aaaattcgga tttgatggag atacagcagc tgtattggtt gcgattgatg aacaatctcc
     1621 tgatattgct caaggagttg atgaagcact tgagatccgg gatgaagata aaaaagacgt
     1681 attagatgag attggtgctg gtgaccaagg tttaatgttt gggtttgctg tagatgaaac
     1741 accagaattg atgcctttac caatcgcttt gagtcatcgt ctggtacgac gcttggcaga
     1801 tttgcgtaaa tcaaatgaat taacgtattt acgtccagat gcaaaatctc aagtaacggt
     1861 tgaatatgat gatcaaggac aaccggaacg cgtagataca atcgttattt caacacagca
     1921 tgatgatgca gtagataatg aaacaattcg tcatgatgtc atcgaaaaag tagtgaagga
     1981 agttattcca gctgaattat tagatgatca aacgaaatat tatatcaatc cgactggccg
     2041 atttgtcatt ggtggtcctc aaggggatgc cggattaaca ggaagaaaaa tcattgttga
     2101 tacgtatggc ggttatgctc gtcatggtgg cggtgctttt tctggtaaag atgcgacaaa
     2161 agttgaccgt tctgccagct atgctgcacg ttatattgct aaaaacattg ttgcagcagg
     2221 gcttgctcgc aaagcagaag tacagctagc ttatgcgatt ggtgttgctc aacctgtttc
     2281 gatctcaatc aatactttcg gtactggaac ggttccggaa gaagaactga ttgctgcagt
     2341 gagagaaaac ttcgatcttc ggccagcagg aatcatcgag atgcttgatt tgcgtcgtcc
     2401 gatttacaaa caaacagctg cttacggtca ttttggtcgt acagatgtag atttgccttg
     2461 ggaacatacg gacaaagtag atgcgttgaa agcaagttta gcaaaataag ttatattcag
     2521 ctggaacgat cgccaagtgg tggttgttcc agcttttttt atggcagtta acctcttgaa
     2581 aaagatagta attatttatc tatttttaga tcctgtcata atgaaaggga ggaataagat
     2641 gaaaaaagaa acaaatgtta ctttggtcac agtgagtatt ttcattgcaa cgttcatgac
     2701 ggctattgaa ggtacaatcg taacaaccgc tatgccaacg attgtcggtt cattacatgg
     2761 aatggaaatc atgaactggg tattttcgat ttatttgctg acaaacgcta tgcttactcc
     2821 gatttacgga aaacttgcgg ataaagtggg acggaaacca atctttattt ttgggacatt
     2881 attgtttatt atcggttcgt tattttgcgg tttatctcaa agcatgattg ggttgattgc
     2941 tgctcgtgcg attcaaggga tgggtgctgg ggcaatgatg ccagtagctt tgacgatcat
     3001 cggcgatctt tattctattg aaaagcgagc aaaagtttta ggtttgaata gttcagcctg
     3061 ggggattgcc agtgtttttg gaccgcttgc gggaggattc atcgttgaaa cgatcagctg
     3121 gcactggatc ttttttatca atgtgccgat cggaattcta ttaattattt tgatttggct
     3181 atatttaaat gaaccaaaaa tcatacatga gtctaaacca atggatattt ttggtagttt
     3241 tagtttgatg gccatgcttg tctcattgct gttagggttc caattattag gagacgaagg
     3301 aatgagtccg cttgtttctg ctttgctgct aggtagtatc gtaatgttag gtatttttgt
     3361 ttttgccgaa aaaagagcta aagatcctgt catcatgatg catttattca aaaatccact
     3421 atttgttgtg gtcaacttgg ctgcagcatt ggctagtggt tttttgatgg gaatcgatgt
     3481 ttatatccct atgtggatgc aaggtgtatt agggctgaat gcagcaattg gtgggctggt
     3541 attagcacca ttatcactag tctggatggt cggttctttc cttgcaagca agtggatgac
     3601 tcaaatgaaa actcattggg tcttgagaat tggactacta atcgcagcta ttgggggcgc
     3661 ttggttgtat cttatgcctt tcagaagtaa ctatttatgg ttttttgcta tatcaacggt
     3721 catcggtata ggcttaggtg tgaccatgac tacctcgact gtcagtgcac aaaatagtgt
     3781 atctcgtgac cagcttggtg tagcaacttc ttttaatacg ttagttcgaa caattggtca
     3841 aacagtcatg gtttctattt ttggtttatt gataaatggg gtaactaaac gagaacttgc
     3901 agcagcaaat ttaacaaatg atgcagatat catgaataaa ctggtgaatc cacaaacggc
     3961 taaatatatt gatgcggata ttttgttacc attaagaaaa attctttatg atgggttaca
     4021 ttccgtttat ctagttggct tgttactcgt gatcattgct ttggtcttaa cgcttttcgt
     4081 tcatgaaaaa agtggtaaaa taaaacagta gtttcggtta agatatgtac gtagccgaat
     4141 cattcaaatt taaagttatt caaaaataac ttcttactaa ggaaggcgaa aatatggcat
     4201 ttattcaagc aaatatttat tcgaatgtgt tagaaatgga agtcaatcta aatgtgatac
     4261 taccgcaaac aacaaaaaag gtgattggca ccagttcaga gcaagagaca acggatatac
     4321 ctgttttata tttacttcat ggaatgggtg gcaatcatag tgtatgggaa agacgtacgt
     4381 cgattgaacg ttatgtagca aactacggat tagctgtcat catgccatct actgatcttg
     4441 gctggtacac agatacagtt tatgacatga agtattggac attcattgca gaagagctac
     4501 ctgagatttg tcatgaatta ttcccgcaat taacaagaaa aagagaaaaa acatatgccg
     4561 ctggattatc gatgggcgga tatggcgcgt taaaactagg ccttgcaaaa tcagaaaatt
     4621 tcgcagcagt aatctctctt tcaggcgctg tttctgtagg aaatcgaatg gatgatcttt
     4681 tgatggttcg taaagcatct ttttgggaag ggatctttgg accattggat caaatcgaag
     4741 gttcaaaaaa tgatccgcaa tacttattga aagaactagt aaaaagcgga aaaccagttc
     4801 caagatttta tatggcttgt ggggaatctg attttcttta tcaggcaaat caggaaatgg
     4861 tatctgctat gcaggaacaa ggcttggcgg tcacctttga acaaggacct ggcgatcata
     4921 actggatctt ctgggaccaa tggattcaac gtgcattggc gtggctaatg gaagaaaaat
     4981 aaaaaaagag tcttaaaaag atagctccta tagagatcac aaacatagtg atctctatag
     5041 gagctttttt actgctcgtt ttctcttctt ttattttgtt ggcataagcc ttcaatatag
     5101 gtttgtgcac tcgttagaaa gagtgctttt tcattcaatt ctgcatggga ctgcatactt
     5161 tctttaaatc cctctaattg attttcaact acataattac gcataaatag aactgtttct
     5221 ttcaaaaatt catttccgtc cataattttt tgtcgaagct gctgttcatg ctgctgatcc
     5281 attaactgtc caatcaataa attgtggaga gaggcaatgg cctgatgact ttgggtgact
     5341 gaaaatcctt cacgaacaag taattggatc atttgctcca aatgttctaa tcgactcgga
     5401 aaagcgggca cagtattgat ttctaattct gccccacaag ggaaacgtgt ataaaggtca
     5461 tagtaatttt ccatatactg cagcaactgt tcattccaag gcaaagttgg atctggaagc
     5521 gctaattctt cttccattgt ttcagccatt atttgcaaga gctgttgttt gtttttgaaa
     5581 taccaatata aagcaggtgc ttgaacatga attctgtttg cgatgtttcg catggaaagc
     5641 tgcgaaagag cttgtgtttc agccaggata tcaaaggcgg cctgaatgat gattgcttga
     5701 gaaagcttta tttccatttt atcaacctcg tgtattgcat tatcttttta aacatggtaa
     5761 attgttagtc ggtctaattt tacagtgtta aattagtcct gtcaatttaa acaaaggggt
     5821 gtaatcaacg aatgaaatta atcttacgac atgcgaaaaa atataaagta gctgttttta
     5881 tttccttgct gtccgtagca gtcatggttg cagcagcatt atggcaacct aagttactac
     5941 agcaagtatt ggaagctatt attacagaag ataatgatga aatgaaaaca attggtattt
     6001 acctgatcag tctggctttg ctaggattag ccgcaggtgt aacgaacacg attttctctg
     6061 caaaagtagc tcaaggcgta agtgcagata tccgagaaga agcatttcga aagatccaga
     6121 cattttcatt tggaaatatc gaacagttct ctgctggaaa tcttgttgtt cgtctaacaa
     6181 atgatatcac gcaaatccaa aatttagtga tgatttcttt gcaatcactt tttcgaattc
     6241 cattcttatt tattggtgcc ttcattttag ctatgatcac gatgccgcaa ttatggtgga
     6301 tcatcgtagc gcttatcgtt acggtatttc tgattacggc actttcattt acaaggatgg
     6361 gcaagcactt catgatcatt caaaaactga ttgatcgtgt gaatagcatt gcaaaagaaa
     6421 acttactagg gattcgtgta gtgaaatctt ttgtacaaga agaaaaccaa ttgaagtcat
     6481 tcagcaaagt aagtgaggac ttaacaaaac acaacatcat agtcggtaca ttattttctg
     6541 tgatgatccc gtccttcatg ctggctgcca atttggctgt ggttggggcg atcttttttg
     6601 tgagcgatct tgttaaagat gatcctacgt taattggtgg aattgcttca ttcatgaact
     6661 acttgatgca gattatgatg gcaatcatta ttggcggaat gatgatgatg atgacttcaa
     6721 gagcagccgt atcgctgaag cggattgcgg aaattttaga tgctgaacca gatcttactt
     6781 atttagacgt tccagaacaa gagttgacgg gttctgtcac ttttgaccat gtttcttttc
     6841 gttatcctgg tgaggataca gatacgctta aagatatcag cttttctatc aaacctggag
     6901 aaatggttgg tatcgtcggg gcgactggtg ccgggaagtc aacactagca caattgatcc
     6961 ctcgtctatt tgatccgaca gaaggcaaag ttgaagtagg tggtgtagat cttcgtcaag
     7021 taaatgaaaa gagtttaaga caaacggttt cctttgtttt gcaaaaagca attttattct
     7081 caggaacgat tgctcaaaac ttgcgccaag ggaaaaagaa tgcaacagaa aaagagatgg
     7141 aacatgcttc ttctattgct caagcaaaag aattcatcga aaagttatct gatcgttacg
     7201 aagcaccaat agaagaacgt agcagtaact tttctggtgg tcaaaagcaa cgtttatcca
     7261 ttactcgtgg cgttatcggt aatccaaaga tcttgatcct agatgacagt acgagtgcac
     7321 tggatgctcg ctctgaaaag ctggtccgag aagcactcga tcgagatttg aagggaacaa
     7381 cgacgattgt catcgcacaa aaaattgctt ctgttgtcaa agcagaccgc atcttagtat
     7441 tggatgaagg cagattagtc ggcgaaggaa cccatgagga attggtagct ggaaaccgtg
     7501 tctatcagga aatctttgaa actcaaaaag gaacggagga gtaagtatga ctgatctaat
     7561 aaaagcaagt aaatttttct atcactattt gaagcggtac aaactctcct ttttctttat
     7621 ctttatcaca gtgatcattg ctacctatct acaagtaaag gcacctcaat acatcgggga
     7681 agcttttcaa gaattagcta attacgtagg aggcttgatg caaggcgtag acgataaaag
     7741 caaatttgtt tctgtaatat ggaaactctt gctgttttat gtactagcta gtgcagccaa
     7801 ctttatctat agtattttgt tcacacaagt agtagggaag tcaacgaacc gaatgcgtat
     7861 cggtctattc aacaaattgg aaaaattgac gatccgtttc tttgattctc atcaagacgg
     7921 tgaaatttta agtcgtttta ccagtgactt agataatatt caaaatagtt tgaaccaagc
     7981 tttgcttcaa gtcatcacga atgctgtttt acttgtcggg atcttgatca tgatgttccg
     8041 ccaaaatgta gagttagctt gggctacaat tgcttccact ccagtagccg ttttgatagc
     8101 tgtcttgatt atccgcaaag cacggaaata tgtggatctt cagcaagatg aagtggggaa
     8161 actaaatggt tatatggatg aaaaaatcag cggacaacgt gtcatcatca caaatggctt
     8221 acaagaagaa acgattgaag gattcttaga gcataatcaa agtgttcgcg aagcaacctt
     8281 caaaggacaa gcttattctg gtctactatt tccgatgatg caaggaatgt cactggtcaa
     8341 tacagcgata gttatctttt tcggcggctg gttagcatta aatggtgatt tggaacgttc
     8401 ggtggcatta ggcttagttg tgacattcgt ccagtattca caacaatatt accaaccact
     8461 gatgcagatt tcatctggat acagcatgat ccaattagcg attacagggg caagacgatt
     8521 gaatgaaata tttgatgaaa aagatgaagt caaaccagaa aactgtaaag catttgatgg
     8581 tgttgaaaaa ggtcttgcgt tgcagcacgt agattttggt tattctccag aaaaactagt
     8641 cttgaaagat gtctcgatca atgtggacaa aggagaaatg gttgctcttg ttggtccaac
     8701 aggatcgggg aaaacgacga tcatgaatct gctaaaccgt ttttacgatg tcaatgatgg
     8761 ttcagtagtc atcgatggaa tagatatccg agattatgat cttgatgcat tacgtagaca
     8821 tgtaggaatc gttttgcaag aatctgtatt gttctccgga acgatcaaag acaatatcac
     8881 attcggcaaa ccggacgctt cagatgaaga agtcatcaat gcagcaaaac aagcgaatat
     8941 tcatgacttc atcatgacgt tagaaaaagg ttatgataca gagatatccg aagaaaataa
     9001 cttattcagt acaggtcaaa agcagttgat cagtattgct cgaacgatca tcacaaatcc
     9061 atctttattg attttggatg aagcaacaag taatgtagac acggtaacag aagcaaggat
     9121 ccaaaaagca atggatgaag caatcaaagg aagaaccagc tttgtgattg ctcaccggct
     9181 gaaaacaatc ttgaatgcag atagaatcgt tgtcttgcgt gatggggaag tcattgaaga
     9241 aggcagtcat cacgagttat tagcagaaaa tggattttat gcggaacttt atcataatca
     9301 atttgtattt gaaagttaaa ttaaaagaac ctcgttataa cggggttctt tttatatatt
     9361 ttatgttgtt tgtttttata aggggcagaa aaggggcaat ttaaatttct atttttccta
     9421 gtttttctac tagttgattt tgcatatttt tagttgtatg agtataaatc tttaatgttg
     9481 tttttgaatc ttcatgtcct acacgatcca taatggcttt taatgggatt cctaattctg
     9541 taagtagaga aatgtgggaa tgccttaaca tgtgagaaga aactttttta tctaggttta
     9601 attctttgga tactgctctt aaagattgat tatatgcttg aatagatatc gggttaccat
     9661 ttttagagat gaaaatgtat ttatctttat tattcttaat taatttgttt tcttcaatta
     9721 attgatctat gatttcttta gcattacttg ataattcaat ggttctataa gaacctgtat
     9781 ttttagttgt tgttttaatt tttaactcgt aatcataagt accagaaacc gtaattttat
     9841 taccatcata atcagattca gtcaaagata tcaattcacc ataacgtagt ccagttaaaa
     9901 acaaaaattt agtaattaaa gaataacgtg actgttgatg tgaacttttt tgtttactag
     9961 caatcatttg aacttcttct tttgataaat aaccatcttt ttcttttttt atttcttcgt
    10021 ttgtcttagg ttttcttgct aaagaaaccc catttacggg attatatttt aatccatatc
    10081 gacggttggc gaatttaaaa atcattcctg t
//
LOCUS       DACDTH010000087        10086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDTH010000087 DACDTH010000000
VERSION     DACDTH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.089"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(247..759)
                     /locus_tag="IWK54_002514"
     CDS             complement(247..759)
                     /locus_tag="IWK54_002514"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9594592.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWK54_002515"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWK54_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9594593.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3646)
                     /locus_tag="IWK54_002516"
     CDS             complement(3212..3646)
                     /locus_tag="IWK54_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9594594.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="IWK54_002517"
     CDS             complement(3656..4444)
                     /locus_tag="IWK54_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9594595.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="IWK54_002518"
     CDS             complement(4441..5382)
                     /locus_tag="IWK54_002518"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9594596.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="IWK54_002519"
     CDS             complement(5559..6146)
                     /locus_tag="IWK54_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9594597.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWK54_002520"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWK54_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9594598.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="IWK54_002521"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="IWK54_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9594599.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="IWK54_002522"
     CDS             complement(9063..9998)
                     /locus_tag="IWK54_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9594600.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DACDTH010000088        10007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDTH010000088 DACDTH010000000
VERSION     DACDTH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.090"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(471..695)
                     /locus_tag="IWK54_002523"
     CDS             complement(471..695)
                     /locus_tag="IWK54_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594601.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            895..3663
                     /locus_tag="IWK54_002524"
     CDS             895..3663
                     /locus_tag="IWK54_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9594602.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            3744..4163
                     /locus_tag="IWK54_002525"
     CDS             3744..4163
                     /locus_tag="IWK54_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9594603.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4175..4645
                     /locus_tag="IWK54_002526"
     CDS             4175..4645
                     /locus_tag="IWK54_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9594604.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4662..5441
                     /locus_tag="IWK54_002527"
     CDS             4662..5441
                     /locus_tag="IWK54_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9594605.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5428..6249
                     /locus_tag="IWK54_002528"
     CDS             5428..6249
                     /locus_tag="IWK54_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9594606.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6266..7315
                     /locus_tag="IWK54_002529"
     CDS             6266..7315
                     /locus_tag="IWK54_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9594607.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7328..8332
                     /locus_tag="IWK54_002530"
     CDS             7328..8332
                     /locus_tag="IWK54_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9594608.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8965..9984
                     /locus_tag="IWK54_002531"
     CDS             8965..9984
                     /locus_tag="IWK54_002531"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9594609.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3359 a         1649 c         2092 g         2907 t
ORIGIN      
        1 atcttttttg ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc
       61 aaagaaggag tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga
      121 ggaataaata atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa
      181 cgaagacaac ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa
      241 aaagaaaaca aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa
      301 actaagagtg gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc
      361 tgaaagcatc ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat
      421 aaggatttgt aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta
      481 tggaaggatt tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct
      541 atatattgcg tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa
      601 ggcaaaaata caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa
      661 gtatgtttcc tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct
      721 ttcatatgtt gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa
      781 attataaggg ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa
      841 agattttatt gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg
      901 aagaaggaaa ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg
      961 aacgaggtct ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc
     1021 agtcattatt tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca
     1081 gtgatttatt tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt
     1141 tatttttcta tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta
     1201 ttctctttta tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca
     1261 gctctttact atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt
     1321 aaaagttatt tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat
     1381 tctgcaccat tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact
     1441 agcaatttgt ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg
     1501 gcttttgaag cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca
     1561 gaaggtcagg aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa
     1621 cctaatttgt cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact
     1681 agtcatttct taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt
     1741 aatcagcgta gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa
     1801 agaaaaatgg ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag
     1861 aaattatccg gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca
     1921 tatgctgaac agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa
     1981 ctacttgcgc agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac
     2041 cagaccttag agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt
     2101 tttgagcaaa tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga
     2161 aaattaaagc aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa
     2221 gaaaaacacg agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg
     2281 gaaactgcct atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc
     2341 tatcagcgaa gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa
     2401 gaattagaac aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt
     2461 ctggaattaa gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg
     2521 acgatctatc taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt
     2581 gctgctcacg gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa
     2641 gaggatctat ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca
     2701 gaaatccttg attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac
     2761 atgggatctc tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg
     2821 attttgaata atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat
     2881 gtatcattag aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg
     2941 ctctatccag aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt
     3001 acggcggtcc acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa
     3061 atcatcccat atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc
     3121 atatacgaag tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat
     3181 ctttcattag aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac
     3241 actactatga atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca
     3301 ttggaaaaag tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc
     3361 gatcatttta tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa
     3421 ttagcgttgt atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc
     3481 gaaacctatc aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt
     3541 aaatctgcgt ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt
     3601 gcctatatct atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt
     3661 tgaacataag caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt
     3721 aagtgaaaag aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat
     3781 ttgcttcagg gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg
     3841 tattcgattg ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta
     3901 tgaggtacaa agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca
     3961 acgaattctt acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc
     4021 cactattgat tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc
     4081 agcaggtgct aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga
     4141 aaatagaaga agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca
     4201 tcggttatta cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat
     4261 tttgattgct aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc
     4321 caagccacaa ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa
     4381 aagcggggtg acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata
     4441 taagctggca gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa
     4501 acctggtact cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt
     4561 gaaacgatta attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa
     4621 agtggatgta agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct
     4681 tattaggaat tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt
     4741 ctcgtccgat tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga
     4801 tcattatggg cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga
     4861 ttccacctga tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag
     4921 ccggtacaga aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga
     4981 aaaatattta cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag
     5041 aagaaggaaa ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct
     5101 tgatgcttgg tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc
     5161 tattaaatat gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc
     5221 cagctcttgg ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt
     5281 ttttcttagg ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt
     5341 ttggtgcgat tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc
     5401 aaacaactgc aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata
     5461 acgaaaaaag agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat
     5521 tatgaacgtc agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta
     5581 tatcagtcga aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg
     5641 accccacata tcgttacact gattttagga atcacggctg caatggagga agaaaataag
     5701 gatgatcctg aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct
     5761 cttgcagggt taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg
     5821 ggtacctctt tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc
     5881 aatattccgc acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg
     5941 ggttttttga aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct
     6001 attattggac tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct
     6061 ttaaaaatcg gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt
     6121 ccaaatatct tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg
     6181 aaaccattaa cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg
     6241 attttttaaa aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa
     6301 ggaagtacta agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa
     6361 aacagtgaat ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa
     6421 gccagcattg gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt
     6481 caattatgga aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa
     6541 aagtgcttct actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc
     6601 tatcacaaat gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg
     6661 tgccggggta gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt
     6721 actactctta gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca
     6781 agactacatg caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac
     6841 ggaagaattt tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat
     6901 tggatatggt ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat
     6961 ccgcgttccg tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc
     7021 agatgcttcg catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca
     7081 aaagttgcaa agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa
     7141 agcaagaaac gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt
     7201 agcgcttgtc gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga
     7261 cttgaacaaa aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt
     7321 ggaaatcatg ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc
     7381 tttacgtccg caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt
     7441 ctattatcta ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa
     7501 tgggaaaagc aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa
     7561 caaaagatta acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga
     7621 agtagtgaaa gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga
     7681 taaagcgaac agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg
     7741 aacagaacaa atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt
     7801 cgaagcttat tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga
     7861 agcagagaaa aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag
     7921 tatgcattat tttattggag cagggaatca atggggagct gtttattcat atgccatgtg
     7981 ttactgggaa gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca
     8041 cgggacgtta gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga
     8101 acagagagtg ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac
     8161 attgatagac acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt
     8221 tttgtctttc ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt
     8281 gaactgccat ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact
     8341 ggataaaaag aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa
     8401 aatattggca tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat
     8461 aatctgttta atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta
     8521 gttgttgcat ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc
     8581 gttttgtatc cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca
     8641 atctgtccgt tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt
     8701 aaatcaatac tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat
     8761 tgtcaaaact gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta
     8821 gactattgta acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca
     8881 cttaaaaaca atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc
     8941 aattatttat ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct
     9001 tggggcactg ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga
     9061 aatgtacctg aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct
     9121 gatttgacga tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac
     9181 gcagatgcta ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt
     9241 attggtaaaa tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat
     9301 acgcataaac gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca
     9361 attgttgtat tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca
     9421 attacagctg caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat
     9481 gattatttca ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg
     9541 aaaaatatca ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca
     9601 aaagcagcaa tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt
     9661 gccaatcctc tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca
     9721 agtgttcatt ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa
     9781 gaagttctag aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta
     9841 gagctagctc aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg
     9901 tataatggag aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg
     9961 gagaatgaat tttctcttcg ttaaatagat gatcaatagt taatcgt
//
LOCUS       DACDTH010000089         9919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDTH010000089 DACDTH010000000
VERSION     DACDTH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.091"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            360..845
                     /locus_tag="IWK54_002532"
     CDS             360..845
                     /locus_tag="IWK54_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9594610.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            838..1521
                     /locus_tag="IWK54_002533"
     CDS             838..1521
                     /locus_tag="IWK54_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9594611.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1647..4292
                     /gene="polA"
                     /locus_tag="IWK54_002534"
     CDS             1647..4292
                     /gene="polA"
                     /locus_tag="IWK54_002534"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9594612.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4337..5173
                     /gene="mutM"
                     /locus_tag="IWK54_002535"
     CDS             4337..5173
                     /gene="mutM"
                     /locus_tag="IWK54_002535"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9594613.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            5137..5766
                     /locus_tag="IWK54_002536"
     CDS             5137..5766
                     /locus_tag="IWK54_002536"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9594614.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            5818..6150
                     /locus_tag="IWK54_002537"
     CDS             5818..6150
                     /locus_tag="IWK54_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9594615.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6462..6953
                     /gene="nrdR"
                     /locus_tag="IWK54_002538"
     CDS             6462..6953
                     /gene="nrdR"
                     /locus_tag="IWK54_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9594616.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            6976..8349
                     /locus_tag="IWK54_002539"
     CDS             6976..8349
                     /locus_tag="IWK54_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9594617.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            8349..9275
                     /gene="dnaI"
                     /locus_tag="IWK54_002540"
     CDS             8349..9275
                     /gene="dnaI"
                     /locus_tag="IWK54_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9594618.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            9404..9844
                     /locus_tag="IWK54_002541"
     CDS             9404..9844
                     /locus_tag="IWK54_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9594619.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         3414 a         1735 c         2183 g         2587 t
ORIGIN      
        1 tttttgcaac ttatgataaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaatc
       61 aaatcaaaaa catgagaagc tgtttttctt ggctgttctt attacttgat gcagggcagc
      121 tttttcacga cctcagataa acggtgttca aaagcttgca tctgcggtaa taagtccagc
      181 caaatgaaaa atatgagaag ctgttttttc ttggctgttc ttattatttg atgcaggaca
      241 gctttttcac gacctctgta aactatagga atctgttaaa tcaagggagc agaacttgcc
      301 ttttacgcta cttttggtac aataatgtat gattaaaaat aaactggagg gtttctatct
      361 tgaacatagg taagccgaga aaactaggaa aaacaataaa tgaaatcgag gaaggcgact
      421 cgttatcctt aacagaatct attgaagata accagctttt gctgtatttg ggattgacga
      481 atgatgcaaa tcctttgtat atccaacatg actttgcaca aaaaacagaa tatcaacagc
      541 caatcgttcc atcagtgatg ctgatgggga tcatcacaag tgcaatttca aagcatcttc
      601 ctggaccagg atcacatgtt gtcaattttt ctgtcaattt tattgaaccg gttttccatt
      661 atgaaacact gacttttcag tttgaggtca taaaagtaga taagatgaag gatgtggtga
      721 cgatttctgt agaagcggag aatacgaacg aagatcgtgt gttggatgct gtcgttatgg
      781 tccaaccgcc acaagttcct gaattggaaa tagcaatcga aagtgaaggt gaattgaatg
      841 aataatccaa gtacatcaat gtcaggtatc aatcgttttt atgcaaaagt ttatggattt
      901 ttaggtatgg gacttgccct tagtgcagtc acttcttact tggctctttg ggtatttcct
      961 ttgcaattag caacttttat caataatttt ccactaggct ttgtgggtct gtggatcgtt
     1021 gaactgattt tagtaatggt tctaggcgta aaagcacaaa agaatccgag cttagcaatc
     1081 ggtggattta ttgcctactc tcttttgaat ggcataacat tagcggttac tcttgcatac
     1141 tacgatattg gaacagtaac gcaagctttc ttgacagcta ctttgatgtt catggggatg
     1201 tctgttgtcg gagtaacaac aaaaagagat cttagcgggg ttggaagagc aggacaggca
     1261 gctttaatcg gagtgatcat tgcactagtc ttgaacatct tcattttgag aagtagcgta
     1321 gttgagctgt tcatctccct actgttagtc gtcatctttg cagggattac agcttatgat
     1381 aaccaaagca tccgtaatat ctatgtccag acaaacggaa cagcacatag tggaattgct
     1441 gtattcatgg cattgaagct gtatttggac tttatcaact tgttcttggc gtttctgaga
     1501 atctttggac ggaacaacta aaaaagaaaa aaattcatca gttggggcta taccgaatta
     1561 tttggtatgg tcccgctttt tcttttcgca ttccgcagga ttctttgtta gaatgaataa
     1621 gaactttaca gtaaggagtt aagaagatga caaaaaataa attattactc gttgatggga
     1681 acagtgtggc ttttcgcgca ttctttgcgc ttcacaattc tcttgaacgt ttcaaaaata
     1741 gaaacggtct tcatacaaat gcaatctatg cattcaatac gatgtttgaa aatgttatgc
     1801 aaaaagagca accgactcat gttctggttg cgttcgatgc aggaaaaacg acttttcgga
     1861 cagaaatgta tgcggagtac aaaggtggaa gatccaaaac acctggcgaa ttcaaagaac
     1921 aaatgccata tatacgtgat ttgttgacag gactaggggt acagtattac gaattgccca
     1981 attatgaagc agatgatatt atcggtactt tggcagaaaa agtagacaaa gatcaatttg
     2041 atgtcgtcgt gctttcaggt gatcgggatt tgactcaatt agctagtaaa gaagtaaaag
     2101 tggatatcac tgtaaaaggt gtcagcgata tcgaaagcta tacaccagaa catgtagcgg
     2161 aaaaatatga tgggttgacg ccaaaacaga ttattgacat gaagggacta gccggagatg
     2221 cctctgacaa tatccccggt gtgactaaaa tcggtgaaaa aacagctatc aagctattga
     2281 aacaatatgg ctcagtcgaa ggaatttatg aacatatcga tgaaatgaag caaagcaaaa
     2341 tgaaagaaaa tctgataaat gacaaagagc aggctttctt atcgaaaaaa ttggcgacca
     2401 tcaatacttc agcgcctgtg gatgtctcta tcgactcatt gaagtatgaa gggaaaaatc
     2461 tcgataaact cgttccgttt tataaagaaa tggatttcaa tcagttcctt tctaaattga
     2521 atatcgtaga agaagaagta aaaatggatg atattttgtt tgaagtcgtt catgaattca
     2581 aagaggaaat gtttactacg gatatggcgt tgtacgtgga aatgatgggc gacaattatc
     2641 atacagagga aatcgtcgga gtagcgtggg ggacagaaaa gaaaatttat gtcacgaacg
     2701 atctatctgt attcgaaaac cgagcgttcc attcttggat cactgatccg actcggctga
     2761 aaaaagtata tgatgcgaaa cgaacttatg ttgctttaaa ccgctatact ggaaaaacga
     2821 aaggaatcga ttttgatgtc ttgctaggtg cataccttct cgatacgaat gaaaaaagca
     2881 cagatatcga aggaatagct gctcattacg gatacaacga tatccaatca gatgaagctg
     2941 tatacggcaa aggggcgaaa aaaggtttac cagaagaaga ggaactcttt tttgctcatt
     3001 tagcgcgaaa agtagctgca atcaatgcgt tgacaccaaa attgagccaa gaactggcag
     3061 ataaaaacca agaagatctt ttccggaaaa tggaactgcc attgtctatc attttggcag
     3121 agatggaaat aagcgggatc aaagtagatg cgactcgctt acaagaaatg aagggcgagt
     3181 tctctgctcg cttaagagag atcgaacaaa aaatctacga agaagccggc gaagaattca
     3241 atttgaattc accaaagcaa ttaggagtga tcttatttga aaaaatggga ttacccgtta
     3301 tcaaaaaaac aaaaactgga tattccacag ccgtagatgt actggaacag cttcgtgaac
     3361 aagcaccaat cgtagaggat atcctcacct atcgtcaaat tgcaaaaatt caatctacct
     3421 atgtcgaggg actgctgaaa gtgatccaat cagatggaaa agtgcatacc cgttatgtcc
     3481 agacattgac ccaaacagga cgattaagct ctgttgatcc taatttgcag aacatcccga
     3541 ttcgattaga tgaagggaga aagatacgtc aggcatttgt tcctagagaa aaagactggc
     3601 tgatttattc ttctgactat tcacaaatcg agttacgcgt acttgcgcat atctcaaatg
     3661 atgaacattt gaaagctgcc ttcctagaag gtcaagatat ccatgccagc acagcgatgc
     3721 gtgtatttgg aatcgaaaag gcagaggatg tcacaccaaa tatgcgtagg caggcaaaag
     3781 cagtgaattt tggtatcgtg tatggcatct ctgattacgg actttcacaa aatctaggaa
     3841 tcagccgaaa agcagcacag caatacatcg atacgtactt tgaaaaatac ccaggtgtca
     3901 aagaatatat ggaaagaatc gtacgcgaag caaaagacca aggctacgtc gaaacgctgt
     3961 atcaccgccg tcgatatctg ccagacatca attcaagaaa ttacaatatc cgttcttttg
     4021 cggaacgaac agctatcaat acaccaatcc aaggaagtgc tgctgatatt ttgaaaatcg
     4081 cgatgatcga attggataaa cgattaaaag aaacaggtct tcaagctacg atgctgttac
     4141 aagttcatga tgaacttgtc ttcgaggtac cggaaaaaga acttgaatca ttagataaat
     4201 tggtgaagga agttatggaa caagctgttt ctcttcatgt accactgatc actgacagca
     4261 gttggggaaa aacgtggtac gaagcaaaat aaacattgtt taagacagag caatataatg
     4321 aaggtgaaaa gaaaaaatgc cagaattacc agaagtagaa accgttcgta aaggattgga
     4381 acggttagta gtaggaaaaa cgattcaaaa agttcaggtc ttgtggccca agatcatcga
     4441 gcaaccagag acacccatct ttgaggcttc tcttgttggc gaaaccatcc aatccattgg
     4501 acgtagaggg aagtttttaa tttttcatct cgatcattgt gaactgatct cacacttgcg
     4561 aatggaagga aaatatcaat ttacgaaaga aaatacacca atcgataaac atacgcatgt
     4621 acttttcttt tttgaggatg gaagccaact tcgctacaac gatgtacgaa aatttggtcg
     4681 tatgacaatc gtcgaaaaag gagcaagtgc aacttaccga ggaatcatga aattgggacc
     4741 tgagcctttg ccagatagct tcttattagc agatttcgca aatggactga aaaagtccca
     4801 caaagcaatc aagccattgc tgctagacca acgtctagtg actggtctag gtaatattta
     4861 tgtagatgaa gcattatggg aagcgaagat ccatccagaa caaccggcga acacactgcg
     4921 ccccaaagag gtagaacaat tacgtctatc gatcattgat gttttggatc gtgcaataga
     4981 agccggaggt accacgattc gcagttattt gaatgcatta ggagaatcag gcggatttca
     5041 agtagcactg catgtctatc agcaaactgg caaaccatgt atacgttgtg gaacgccgat
     5101 cgtgaaaaca aaagtggcac aaagggggac gcactattgt ccgaattgtc aaaggctaaa
     5161 aatcgcaaag taggatttgt tttaggtttg acgggtggaa tagctaccgg taaaagtacg
     5221 gcagccaaag tctttcagtc tcatggattt cctttgatcg atggcgatgt gattgccaga
     5281 gaaactgttg agcctggaac gcttgctctg aggaaaattg tatcagtgtt tggccaagag
     5341 gtattgcagc ctgatgggca actagatcga gggaaacttg ggatgattgt ttttccatca
     5401 aaggaaaaac ggcaaaaact tgatcagcta ttagatccgt ttatccgaaa agcgataaaa
     5461 gatcaaatcg cctctctaag ttccaatcat cctttagtaa ttgtagatat cccattgctc
     5521 tacgaaggtc attatgacca ctatatggat gcagtagctg tcgtttatac tacgccagaa
     5581 actcagcttc agcgtttgat gaaacgtaat caactgacgc tagaacaagc gcagcagaga
     5641 attagtagtc aacttccaat cgaagaaaaa aagcagcgtg cggatatttt gttcgacaac
     5701 aatggaacaa aagaaaaact tgtgggacaa atcgaaaatt ggctttttgc taatcgcttt
     5761 ctttgagtat ctgtgtcaat tttgctatcc tgagtatata gaaagtgagg gatagcgatg
     5821 ggatcattaa aaaatcaaaa ggataaagtc gtaggtaacg caaaagaaaa aatcggcgag
     5881 atgctcaata aagaaaatct aatcgatgaa ggtcgtacgc aatcagaaac agcagaagaa
     5941 aaagaaaagc agtaccaagc aagaatcgat gaaatagaag accagcaggc agaagaagaa
     6001 gcgaagcggc aagatttgac tcaagaacga aaaaatccga cacctgatcg tagtaaaaca
     6061 ccgctcccag aaagtgacca catttccgat gagcaaaaaa tcgaggaaca gcgaatgaaa
     6121 cactatacag gaatcgaaga gaagttataa aatagtaaaa aatcgagatg tgacttgaag
     6181 cagatgagaa ggtgttcatc tgcttcaagt tttttgtgct agatgactaa atcagctttt
     6241 gtgtaagtta tttctcagta atcaaggtga tcagtattgg aaaattgaaa tttagtaagg
     6301 acaggggaca caattacgca tgaaagtaga aaaaattgtt cttatatccg tgttttttgg
     6361 gtaggacagt cgcgtttgtg atttgtttcg tgttataata caactagttg taaagaaaga
     6421 cacatccttt atataaaatg cgatgcaaga ggtgagacaa aatgagatgt cccagatgtc
     6481 aacataataa ttcacgagta atcgacagcc gtcaagcaga tgatggacgt gcgatccgtc
     6541 gccgtcgcga atgtgagaat tgttcgttcc gattcactac atttgaacga attgaagctg
     6601 ctccgttatt agtaatcaag aaaaacgggg aacgcgaaga gttcaatcgc gataaaattt
     6661 taagaggact catccgttca gcagaaaaaa gacctgtagc tatggaacaa atggaacaaa
     6721 tcgtcaacca tgtcgaaaac cgcgtacgca gtctcggaga aaacgaagta tcgacgaatc
     6781 taattggtga atatgtgatg gaagatttag ttgatttaga cgaaatcgct tatattcgtt
     6841 ttgcaagtgt ttatcgccag ttcaaagata tgtctgtttt cttaaaagag ttgcaagata
     6901 tcgtggataa agcaaaagaa aataataaag agaataataa agaaaacaat taagaaacaa
     6961 aaaggaggca tttccttgga aaatgcttgg aaagagcttc aaccaaaaaa tatttaccaa
     7021 ggaagaaaag cggctccgct gagagaagac agcaagaacg ccttgctgta tctttatcag
     7081 ccactaatcg gcggagaagc cttgagtatc tatttcaccc tgttatcaga aatctctcaa
     7141 gaatctggca aaggtcctga agggatgcat gcagatctgc ttagtagtct aggctgcggt
     7201 atcccgcaat tttatgatgc aagaaaaaag ctagaaggaa tcggcttact ggatgtttat
     7261 tttaaagaag atagtgatct gggacctcgt tttatttatg agctgttaga accactcatt
     7321 cctaaagcat ttttcaaaga ctctgttctg gcttttcttc ttctggaaaa agttggtgaa
     7381 cgacgattcc agaagcttac ccaattcttt gaacctaaaa ggcatacagc agagggatat
     7441 caaaaaatca caaaaaaatt tttagatgtt tatcgcttca atgaagcagt gtacgctgct
     7501 aatcaagaaa acatcgaatc gctgaataaa tcttttgaag aatctgaatc agtagcatta
     7561 cttgaagcag attcgacatt cgattggcag tttttgaatg attggctggc aaaaaaacat
     7621 attgcaagtc ttgatgatcc aatcatcaaa caagtcgaca tgtatcatca gctttacggt
     7681 ttcgatgaat tgacacttgg cgaactgatc agtcaatctt atgactttag tgaacaaaaa
     7741 gtatctgaaa aagaactgca aaaagccatt ttggcaatgc agagacgtcc ggtgaagcaa
     7801 acaaaagaaa cagttgcggt ttctgaaaac agtaatgaac cctcgtcaaa cgaacatttg
     7861 acaaacgcag cgcttcagct gataaaagaa gcacagagca ttccaccgat gaaatatctg
     7921 gaagcaatca agtcagaaaa aggcggatat gtttctaaac aagaaaactg gttactgcaa
     7981 gatcttgttt ctcaatcagg tctttccagt agcgtgatca atgttttgct gaattatgta
     8041 ctagtgatca agaataatgc tagcttaaat gccagttatg taaatacagt ggcaaatgaa
     8101 tgggcacaaa agaaaatcgc cacagcagaa gaagcgatcc agcacattcg gcaaataagt
     8161 aatcaagcaa aacaatcaaa acaaaagaag caaacaaatt atcaaactgg caagcgaaat
     8221 gttcgacggg aaaagcttcc tgattgggtc aaccagccaa aagatgaaaa acagatcagc
     8281 agcgagaaaa aagcagagat tgaccgccgt tttaaagaat acctggcaaa aaaggagggt
     8341 gatatctagt ggaagacgta ggcaaagaat tgaagaggat catcagcaaa cgcaattatc
     8401 aagaaaagta tgctgaaatg atcgctgaag tcttaaatga tgcggatgtt caagcttttc
     8461 taagcgcaca ccaagaagaa ttgacacaag cggatattga aaaaagttac gctaaattgt
     8521 atgaattcgt gcaagaaaag cgtaaatatc aaaaaaacga tccaacgatg atcgctcccg
     8581 gctatgagcc gaagctgacg ttgaattttc attatgtgga tgtcacttat gtaccaacgg
     8641 aagcattgtt agcacggcaa agacaagaag aaatcagaag ccgagtaaaa gcaatggata
     8701 tgccaaaaga tattcaatcg gcttcttttg aaaactttga acgtacagat ggacgaggat
     8761 atgcgagctt agaagcccta gattttgtag agaaatatac tgctgatcca aaagggtatc
     8821 ataaaggact gtatctagta ggaagttttg gtattggaaa aacatattta ttaggagcaa
     8881 cagcgagaga attagcgatt gctggattca ccacgacgct agtccatttt ccgacatttg
     8941 ccgttgagat gaaacaggca atcggaaaag atcaagtggc agaaaagtta gatgcagtga
     9001 aacgttcacc tattttgatg atcgatgaca ttggtgcaga tgcgatgagt agttggatac
     9061 gagacgaagt attcggtgtt atccttcaat atcggatgca ggaacagtta ccaaccttct
     9121 tctcatcgaa ttttacaatg gatgaattgg aacagcattt gtccgtcact caacgtggag
     9181 atgaagagcc gctaaaagcg aaacggataa tggaacgcat acgttatttg acaaaagaaa
     9241 tcgaaatgac tggtcgcaac cgacgcaatg gttgaacagt tgacaagtcc aactgacatt
     9301 tgacctagta gtaaaagcag gtcagcgaat cattatatgg acagaaatga ccggagacaa
     9361 tcaccgcaac aagtaatgca gaaacttaaa cggaggattt tttatgaggg ctattttttc
     9421 tagtatatct gtagttggtc tattttttct tttagcaggc tgcggtagcg cagatagcaa
     9481 cacaggttct gttgattcga ttgatgcggg tcaaacagta aaaacaagca gtacgaaaac
     9541 ggagagacaa agcatatttg tcggaacgat tgaaagtatt actcaagtag aagcagaaga
     9601 agaaacgctt cagttgaaac tcgtcgatgt catggccaaa gaagatccag cgaatatggg
     9661 aacgtcattc agcaatgacg gagtgatctt aaatgctact gctgaaaaaa taagcgtgga
     9721 cataaatgaa ttgaaaatcg gtgacaaagt ccaatttaca ttagttgaga tgccgatcat
     9781 gacgatgtct atcccaccgc aagttcctgg agattcgatt attgatgttt cattgaacaa
     9841 ataaccaata acattttttc aaaaataaga aaatgttgtt ttatagaacg aaaaggtata
     9901 ttttttaagg agatatggg
//
LOCUS       DACDTH010000090         9865 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDTH010000090 DACDTH010000000
VERSION     DACDTH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.092"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            7..237
                     /locus_tag="IWK54_002542"
     CDS             7..237
                     /locus_tag="IWK54_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9594620.1"
                     /translation="MVNFYLFCLMGYDKHQARTSGWRVPENNLIFVSLLGGGLGGLIS
                     RKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            248..1294
                     /locus_tag="IWK54_002543"
     CDS             248..1294
                     /locus_tag="IWK54_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9594621.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            1545..4154
                     /gene="clpB"
                     /locus_tag="IWK54_002544"
     CDS             1545..4154
                     /gene="clpB"
                     /locus_tag="IWK54_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9594622.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(4305..4976)
                     /locus_tag="IWK54_002545"
     CDS             complement(4305..4976)
                     /locus_tag="IWK54_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594623.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(4969..5463)
                     /locus_tag="IWK54_002546"
     CDS             complement(4969..5463)
                     /locus_tag="IWK54_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9594624.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(5483..6703)
                     /locus_tag="IWK54_002547"
     CDS             complement(5483..6703)
                     /locus_tag="IWK54_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9594625.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            6850..8685
                     /gene="lepA"
                     /locus_tag="IWK54_002548"
     CDS             6850..8685
                     /gene="lepA"
                     /locus_tag="IWK54_002548"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9594626.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(8779..9861)
                     /locus_tag="IWK54_002549"
     CDS             complement(8779..9861)
                     /locus_tag="IWK54_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9594627.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         3102 a         1845 c         1985 g         2933 t
ORIGIN      
        1 cttttcattg tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga
       61 actagcggct ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt
      121 ttgggaggat tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact
      181 ataggattcg ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga
      241 gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat
      301 tgtgattttt gccattattt attatttaat caatcaatca tttgcagata tctttgccga
      361 attgctgtct accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca
      421 aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac
      481 gaaagatggg ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac
      541 aggaacgctg atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg
      601 agcaggggtg acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt
      661 agctggcttg gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt
      721 tatcatcgct ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt
      781 cagcttgaat gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg
      841 tttgcggaaa tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca
      901 aaccattatc caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact
      961 tgtattttgg tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt
     1021 tctactgacc atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc
     1081 tgctggtatt ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac
     1141 agttgacgca gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct
     1201 ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga
     1261 agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta
     1321 tcaaatcaaa gaaaaacgtt atggataaga taattttacg tttgtttggc tcttttttta
     1381 tatacataca atcaattttg tttttttaag atttatataa gtcgagaaaa aaagtcaaaa
     1441 aactatgtgt atattttgac caaaattgac caaagcgata taatagtttt tgtaaagatc
     1501 aaagaaataa gtgatcttac ctttaattag gagggtatac ctgtatgaat attgagaaaa
     1561 tgactacgac gcttcaagaa gcgattgctg aagctcaaca gattgcggtc actcgaaaac
     1621 atcaagatat cgacattgcc catgtatgga aaatcttttt acaacctaat cattttggtc
     1681 gaaatttcta tacggatgcc ggattggatg tcgaatcttt tgaacatgaa attgatcgtc
     1741 tactggatga gtatccagtt gtttctggtg gaaacgtcca atatggacaa aacctcagcc
     1801 agaatttctt tcgattattg aatgaagcag accaaatacg tgaaagcttc ggcgacgagt
     1861 ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat ccgttaactg
     1921 tttatttgaa taaaaacgga ttatctgaaa aagaattgcg taaaaacatc gaagagatga
     1981 gaggaggaga aagagtgacc tcacaaaacc aagaagaaca atacaaagca ttagaaaaat
     2041 acggcgttga tttagtacag caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg
     2101 atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat aatcctgttc
     2161 tgataggaga acccggtgta gggaaaacgg caatcgttga agggcttgct caacgtatcg
     2221 tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat tttttcatta gatatgggtg
     2281 cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa gcagtcttga
     2341 aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg
     2401 taggggcagg aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg
     2461 ctcgcggtga attacatttg atcggtgcca caactttaga tgagtaccgt caatatatgg
     2521 aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg
     2581 aagatacgat cagtattctg cgtggattaa aagaacgttt tgagatccac catggtgtta
     2641 acatccatga caacgcatta gtagcagcag ctactttatc tgaccgttat attactgatc
     2701 ggttcttacc ggacaaagca atcgatttga tcgatgaagc cagtgcaacg attcgggtag
     2761 aaatgaactc tatgccaacg gaattagatc aagttactag acgtttgatg cagttggaaa
     2821 tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt
     2881 tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa
     2941 cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac
     3001 acgaattaga agatgcagaa aataactatg atttagaacg cgctgctgta ttgcgtcatg
     3061 gtacgatacc tcaattagaa aaagaattga aggaattaga agctaaagca aaagacagcg
     3121 agattaagat ggttcaagag tccgttaccg aaaatgagat tgctcaagtc gttggcagat
     3181 taacgggtat ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta attaaattga
     3241 atgaaacact tcataaacga gtgattggtc aagatgaagc agttgatgcg gtcagtgacg
     3301 cggtaattcg ttcacgggct ggattgcaag acccaaatcg tcctctaggt tctttccttt
     3361 tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa agcgttagct gagaacttgt
     3421 ttgactcaga agatcatatg gtacggatcg atatgagtga atatatggaa aaacatgctg
     3481 tgtctcgtct agtcggagct cctccaggct atgtaggtta cgaagaaggt ggtcaattaa
     3541 cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc
     3601 atcccgatgt cttcaatatc ttgttacaag tcctagatga cggacgcttg actgattcaa
     3661 aagggcgagt agtagatttc aaaaatacgg ttctgatcat gaccagcaat atcggttcac
     3721 agttactgct ggaaggcgtg actgctgatg gaacgattcc agaagcagtg gctgaacaag
     3781 tcaacacgct attacgtgga aacttcaaac cagaattctt gaaccgaatc gacgacacga
     3841 ttttatttac accattgagc ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc
     3901 aattggcaca acgtctggaa catcaagaaa tcctgctgac aatcagtgat gaagccaaaa
     3961 cgtggattgc tgaaaatgct tatgaaccag catatggtgc aagaccattg aaacgattta
     4021 tcactaagga agtagaaaca ccactagcta aagaaatcgt tgcaggacat gttatgccta
     4081 aatcaaaagt aacgattact ttacttgatg gacaacttca tttcaaaaca gaagagctag
     4141 aagaaatcgt ctaatcttcc attgagacta taaaagaaaa aaggggtctg acacaactag
     4201 tgtcagacct ccttttttca aataaacagt gttccagaag tagtttcttc gaaaaataag
     4261 ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct tttattaggt atttttcact
     4321 ttttctaatt tataactttc aaagtaacgc tcatcttttt tactttgaga ccatgcaaac
     4381 aatcccatta aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt atccatcaat
     4441 gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa aacgttgaag attcatgcct
     4501 aagaactggt cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac
     4561 gcaaagccga ataagaggaa caaccaagga ctggtttttg atatctcagc atctttcatc
     4621 aagtaaccaa tcaatccata gccaagagct gtcaggacca ttggactcac cgcatgtaag
     4681 attttgtccc aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa aaagacagag
     4741 atgaacaaga aaaaccagta aaacaaaacg attgctgctg gtattttaag tttgaaaact
     4801 ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta ataaagattc cacgataaat
     4861 gtgattcctt gagaagtcga atatttttgt gtgataaatt cataaaaatt atagcagaca
     4921 acgaatacac caaaagcaag taaagctatt tgcacctttt tgatttcttt aatcactgaa
     4981 agtccctcca tctaaaaatc tttttacttc tgaaagcaac tcaatttctg aagaaaccac
     5041 ttttccgttc aatttgatca aacctattgt atataagttc aggtagtgaa attgattttc
     5101 agctgtttct tgtagtgcag cgattttttt cgggtgggtt ccgccttgct gtcgagtatc
     5161 agtatataag ccgatcactg ggatgccttt cgcataagcc acaccgattt ccgatgctac
     5221 cccggcatcg atcgtcagcc catctagtaa agcaatcatt aaatcactag ccaacacttt
     5281 ttctgtgtca gcaagagcaa tcattcgact gtctgcataa gcagttttgt cattgattgc
     5341 ttcattttct tgaggaagat agatcgaaag ctcagaagag agctctctga tttctttaac
     5401 taactgttgg ttatataaca aatcactttt agcaaacatt ggtgcggcaa aataaatttt
     5461 catctaaaca tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa
     5521 tgatacgctc tagtgacttc agtgtttctt catctgtttc taaccgttcg attcttgcca
     5581 aataagaagc acggcgataa ttcatcatca agcaagtcaa tgtttggata tccagtcgaa
     5641 cggattttcc tataggttca tttaagacac ggacttgtcc ctgttcatcc catgttaaag
     5701 cgaagacacc attattccaa ggagcgacag gatcttcaat gatgaaatga aaagcatcag
     5761 ctgttccaac aaacggaaac cgttgaagga attcttttac atcaacgatc cgagccataa
     5821 aatatggttc gatctgctct ttgatctgac tatcttcaat caaaaatgct aaaggctcat
     5881 ttttataaat atctccatgt acccagtaga tcatggaaaa atgtgcggaa ataaagttcc
     5941 ataagccatt tcgagcttct tgatcaagat agaacatttc ttttacgtga aatatttctt
     6001 cagctaccca atagaacaga acacctcttg gttcatggtt gctatcgtag tagacagcgg
     6061 ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga
     6121 ataaagcacc atggttttgt tgggcaaatt tgtcataaac ggaaaatacg tctggatgat
     6181 caactggtaa tcgctcaacc ataccactta ctgggatttg tttaggtagc tgtgtatctc
     6241 tgattttaaa ggaaagttta tcagacatga tttcccatcc tttacgccga tagtagggga
     6301 tactgtaagg atacaaataa gagatccatt gcttattctt gcgcatatta tccaatgcaa
     6361 cgataattag atcttgcatc aatccatggt tagcatattc tggataagtt ccaactccag
     6421 tcacaccacc cattttataa cgtgcaccgt gaatattgac ttcacaagga tagatagcga
     6481 tttgagagat cagtttattt tcgtggaacc atccgaacac tttcgatact tccaaaatcg
     6541 gttttttgga acggataaaa gctcgtttat tctcaaaccc gctttcctcg atgtctgctt
     6601 cagtcacttg gaacacatag gaaagcaatt cattgaattg atcgatatgt tcttgagtga
     6661 ccggttttaa ttcaagattt tctctaaaat ctttttgatc cattttttca tctccattta
     6721 ttagcttgat aacagtatag caaaattcta caaaaaaaac ttctatagct gactaattaa
     6781 aaactcttta aattactgct tttcgttggt ataatggaaa gtgctgatta tatgaaaaga
     6841 ggagacgcta tggatataaa gaaaatgaaa gaacgccaag agcagattcg taatttttcg
     6901 atcatcgctc atatcgacca tgggaaatct acattggccg atcgtatttt agaaatgacg
     6961 cacactgtga cgtctcgcga aatgcaagat cagctgttag actcgatgga tttggaacgt
     7021 gaacgtggaa tcacgattaa attaaatgct gtagaactca attacacagc gaaaaatgga
     7081 gaaacatata tctttcactt gattgacacc ccagggcacg tcgacttcac ctatgaagtc
     7141 tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc
     7201 gaagcacaaa cattggccaa tgtttattta gcgttagaca atgatttgga gatcctgcca
     7261 gtcatcaata aaatcgacct acccgcagca gatcccgaac gtgtccgtca agaaatcgaa
     7321 gacgtaatcg gaatcgatgc tagtgaagct gtattagcca gtgccaaatc agggattggg
     7381 attgaagata ttctcgagca gatcgtggaa tatgttcctg caccaagtgg agacttagat
     7441 gcaccgctaa aagctttgat ctttgactct atttatgata gttaccgcgg tgttgttcta
     7501 aatgtccgga tcacggacgg tatggtcaaa cccggagaca aaatcaaact gatgagtaat
     7561 ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc caaaagcagt tgctagagac
     7621 tttttgatgg tcggtgatgt cggttatatc acagcaagta tcaaaactgt ccaagataca
     7681 cgagtagggg atacagtaac gttagcagac aatcctgccg ctgaagcttt accaggttac
     7741 cgaaaaatga atccgatggt ttattgtggt ctttatccaa ttgatacttc tcgttacaac
     7801 gatttgcggg aagctttaga aaaactgcaa ttgaatgatg ctgctttaca attcgaacca
     7861 gaaacctctc aagcattagg ttttgggttc cgctgtggat tcctcggact tcttcatatg
     7921 gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag aattgatcac gactgcacca
     7981 tccgttattt accatgttaa taaaacggat ggtacgactg ctacagtaga taacccggca
     8041 gattttcctg aacctgtaac gattcaggat gtagaagaac cattcgtcaa agcacagatc
     8101 atggtcccaa atgattttgt cggtgcagta atggaacttt ctcaacgaaa acggggagaa
     8161 ttcattacga tggattattt agatgattac cgagtaaatg tcgtctataa tattccgctt
     8221 tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca
     8281 ttggattatg aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga catcttgctg
     8341 aatggagaaa aagtcgatgc attaagcttt atcgttcacc gagactttgc ctatgaacgt
     8401 ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca
     8461 atccaagcag ctattggaca aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa
     8521 aacgtcttgg ctaaatgtta tggtggagat gtttctcgta aacgtaaact cttagagaaa
     8581 caaaaagaag ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc
     8641 ttcatggccg ttctgaaaat ggacgaggac gaaccgaaga aatagcaagg gtttgtttga
     8701 aaacagtgta aaaaataaac catgtttaca tggatttgcc accggtttgc caccggtggt
     8761 ttttttcggt ggcaaaaatc aagaattctt tctaagattt tccatgatat cgaccgtttc
     8821 atttctcatc ttatctgtta catgcgagta agtatccatc gtaattgaaa ttctgctgtg
     8881 gcccaatcgt tcagagattt ctttcatttt tgcgccattt tcgagaagaa gtgtagcatg
     8941 agtatgtctg agagaatgga agttaaaaga gagggagagt gcattcgata tcctcctagt
     9001 attccatttc actacacttg gcgtaactag ctcaccgtct tccttcgtac atactgcatt
     9061 tgaatcaatg tagagctttc catacttcat tcgattttct aattgttgtt tcttatgttt
     9121 tttcagaatt gctagcaagg tttgtccaat aaaaatcgtt cgattggaac tgcttgtctt
     9181 tggcgtacca tatacccatg cgccgtcatt ctttaccatt tgtttctcta cagtaattgt
     9241 tccatttgaa aaatcgacat catcccacgt cagaccacaa acttcgccaa cgcgcattcc
     9301 cgtataaaat ccaatattca aaggaatata gaaaggatgt ccttcaggag taatttctag
     9361 catatgatca aagtcctcaa gagaaatgat ttttagatct tttttagtcg ttggtcgtgc
     9421 ttcatatttt ggtattttta catacagcat aggattttgc ttgattaatc cccaaggata
     9481 aaccgccata ttcagcgcat tcttaaggac agagtgagta atagtcattg ttttcttcga
     9541 gtaacccttt ttaaattcag cattgatgaa attttgtaaa agagcagggg agagatccgt
     9601 aagttttttc tttcctaaat aaccgtttat atgatttttg atggtaaatc ggtagttttc
     9661 ataggtattg tattttagat ttagtttaac gtattcctcc atccaaaaat caaggtattg
     9721 ttttactcga gtatccgtac ctaaaaagta ttgtcctgtt tcgtcaatat ctgataaaac
     9781 ttttcgtaaa gcagcttcgg cctctggtcg ggtgtctccg ccaactttct ccactttttt
     9841 tcttgagcca tcatcattga tatct
//
LOCUS       DACDTH010000091         9536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDTH010000091 DACDTH010000000
VERSION     DACDTH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.094"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..308
                     /locus_tag="IWK54_002550"
     CDS             <1..308
                     /locus_tag="IWK54_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594628.1"
                     /translation="VTSADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQ
                     LFDTMLKDGQVTATATYDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            354..1298
                     /locus_tag="IWK54_002551"
     CDS             354..1298
                     /locus_tag="IWK54_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9594629.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1314..2093
                     /locus_tag="IWK54_002552"
     CDS             1314..2093
                     /locus_tag="IWK54_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9594630.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2105..2803
                     /locus_tag="IWK54_002553"
     CDS             2105..2803
                     /locus_tag="IWK54_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9594631.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            2831..3595
                     /locus_tag="IWK54_002554"
     CDS             2831..3595
                     /locus_tag="IWK54_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9594632.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            3639..5090
                     /locus_tag="IWK54_002555"
     CDS             3639..5090
                     /locus_tag="IWK54_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9594633.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5122..6258
                     /locus_tag="IWK54_002556"
     CDS             5122..6258
                     /locus_tag="IWK54_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9594634.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            6258..7364
                     /locus_tag="IWK54_002557"
     CDS             6258..7364
                     /locus_tag="IWK54_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9594635.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            7370..8392
                     /locus_tag="IWK54_002558"
     CDS             7370..8392
                     /locus_tag="IWK54_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9594636.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            8414..9469
                     /locus_tag="IWK54_002559"
     CDS             8414..9469
                     /locus_tag="IWK54_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9594637.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT         3497 a         1330 c         1981 g         2728 t
ORIGIN      
        1 aggtgacaag tgcagatgtc gctcgtgtac gtgaaaatat gagctttttt aaagatagtc
       61 cttggaaaac ggttgttatg caatatctag gattcgctga tattcagatc gcgcaagtca
      121 cgcaactaga tcagttattc gatacgatgt taaaggatgg tcaagtgact gccaccgcta
      181 cttacgatca atatttgacg gtgctcagtc aaatagagca gatccggaat gaaaaaataa
      241 gcgcagcata cgcaacaaaa gcacaaactg ttgctgagca aatgggctat agcaatataa
      301 gctattgaca gttaaaacgt tgattcaagc aaggttagca aacggaggaa actatggcaa
      361 gacgaagaag aaaatcaaga gggaaaaaaa tatttctgat tattttagca gttcttatcg
      421 tattagtatt agcaattgta ggagtaggag caaaattata tctggatctc tcacattcta
      481 ttgagaaaac ttacgaatca gtggaacgaa cccaaggaga acgaacgaaa ccagttgatc
      541 tggacaaaca agaatctttc tctgttctat tgatggggat tgatactgga gatttaggtc
      601 gggtcgatca aggaagatca gatacgatga tggttgccac agtcagtcct gaagacaaac
      661 agacaacgat cgtcagcatt cctcgtgata cgtatgtcga catcgtaggt cgtgacacaa
      721 aagacaagat caaccacgct tacgcattcg gcggagctgc catggcgatg gataccgtac
      781 aaaaatatgt agacatccca atcgatcact atgtctctat caatatgaaa gggctaaagg
      841 aactcgttga tgcagttggt ggtatcgaag tcaataatga cctcactttc tcacaagatg
      901 gctatgactt tccaatcggt aaaattacat tgaacgggga tcaggcatta tcttattctc
      961 gtatgcgtta tgaagaccca aatggtgatt acggtagaca agggcgccaa agaaagatcg
     1021 tagaaggcgt agcgaaaaaa gtattgagtc taaacggtgt cagcaactat caatccgttt
     1081 tgaatgctct agagtcgaat atgaaaacag acatgaactt tgatgaaatg aaaaaaattg
     1141 ctttagatta tcgggaggca tttggtacga ttaagcaaga tcagctgcaa ggagaaggct
     1201 tcatgcaaga taatatctcc tatcaaaaag tcagtgatca agaacttgat cgtgtgaaaa
     1261 aagaattaaa agatcaaatg aatctagaga ataaatagga aagtggagaa aaaatggaag
     1321 agacaatcag cttgaaagaa atttttgaaa cattacgcaa acacttggta ataatcatca
     1381 tctcaatgtt tgctggcttg gccatctcag gaatcgtaac atttttcatc ttgacaccaa
     1441 aatacagttc acaagcacaa ttgatcgtga cactgccaca atcagacact gcaaatgtca
     1501 atgatgtcaa tacgaacctg caaatgatca atacgtataa agatatgatc gtcagtgatt
     1561 tagtgctcaa tgaagtcaaa gatcgtttag aaacagaaga caatgtgaaa atgactgctg
     1621 aacaaatcaa agatgccatc tcagttaatc aggcagaaaa ttcacaaatg ttctcgattc
     1681 aggcaataag tacgaatgca gtggctgctc aacagatcgc taacacaact tcacaagttt
     1741 tccaagagaa cgcaaaagat gtgatgaatg ttgataaaat ctcgatcatt tcaggtgcag
     1801 ttgccaatac gacacctgtt tcgccaaata ataaattgaa tttaatgact ggcttaatgt
     1861 taggaatgat ggtaggtgta ggtctggcat tcttattgga attactcgat cggacagtga
     1921 aagatgaaaa atatgtgaca gatacattag gattcccaat cttaggaaca gttccagaaa
     1981 tgtcagcgaa agaattgaat gctggtatcc gtaaaaaagg accagctcag acacaaccaa
     2041 aagctacaaa cgataaaaca gcgccatcac gcagaagccg ctcaagagtc taggaggaaa
     2101 gaaaatggca cgaacacaga aacaaaaagc agcccaaact catgcagtca gtttggtcac
     2161 actcaccaat ccctcttcac caatcgctga acaatatcgt acgattcgga cgaatatcca
     2221 gtttgcctcc gcagctggtc agcaaatcaa aacgattgtt gtgacttcat caggacctgg
     2281 agaagggaaa tcgaccactg cagcaaacat tgccgttgtt tttgccaaat ctggtcaacg
     2341 ggtgctgcta gtcgatgcag acttacgtaa gcccgtcatc tataaaacgt tcaagttgaa
     2401 caacgcaagc ggattaagta ctgttcttag ttcatcagga agtgtagcag atgagatcca
     2461 acgaacacca gtcgacaatt tgtcgattct gccaagtgga cctaaaccac caaatccatc
     2521 agaactacta agttcgccaa gaatggatca gattttagca gaagcaagac aactatttga
     2581 tgtagtgatc ttcgacatgc cgccagtcgt tgccgtaaca gatgcacaga tcatgtcttc
     2641 aaaaacggat ggcaccttat tggtcgtacg ggaaaatacg tcacgaaaag aatcattgac
     2701 taaagcaaaa gaactgttag atatggtaca agcacgagtc ttaggtgtcg tatacaacgg
     2761 agcagaacac agcaaagatg ctggttatta ctacggaaat taaggaataa gaagagagga
     2821 gacaagagaa atgatcgatc tacattgcca tatcctacca ggaatcgatg acggggcaga
     2881 aaacttagaa gcaagtattg caatggctga aaaagcgatt cagcaaggca ttacgcatat
     2941 tttatgcaca ccacaccaca ataatggaaa atacagcaat gaaaaatcac aagtcatttc
     3001 tctagtcgct tcgttacaag ccgaattaga gaaaagacag ttgcctttga cgttattgga
     3061 aggacaagaa gtacgtatta ccggaacgct catcgaagac atccaccgag atgaaatttt
     3121 atttacagat ttagatgata catatcttct gatcgaattt ccgacactgg aagtgccgtt
     3181 atatgcagaa cggctttttt ttgagttgtt agaactagga aagataccag tgatcgtcca
     3241 tccagaacgc aacgcgcatt ttcgagaaga cccaaatcat ttgcttccgt tcctagatat
     3301 gggatgtttg gcccaactta ctgcgccaag ttatgttggt gtatttggca aagatatcca
     3361 gaaaacagcc aaaacaatgg tcaaacataa tctcgtgcag atggctgctt ctgatgcaca
     3421 cggggtctct aagcgcacct tttacttaaa agagtgctat gagcagattg ccaaggagtt
     3481 tggtaaggaa aaagtagtac agatgaaaca agtcgcaaag gatctgatca acggggatga
     3541 gatcaattat ccaatctatg aagaggtaaa aaagaagaag tttggtttat tttagttctt
     3601 tattcgtaga taaaaaggaa atgaggagag agtgagagat gtatagaaaa aactcaagtg
     3661 gttggtctaa acatattgat tttattttat tagacttatt ttgtgtccag cttgcttttt
     3721 atatatcata tgtaatgcga cagggtgact ggaatccgta tgtgattcct atttatcgcc
     3781 acatggcaat ttttatagaa cttgagaatc ttacgattat atttttattt gaggcatata
     3841 aaaatgtttt aaaaagagga tattataaag aggctgtagc ttctataaag caatctttta
     3901 tgctgttact tgtgtgcagt ttatacctta ttacaatgca ggatggaaat gattattcca
     3961 gagtttcttt atttataatg ggggtggtgt atggattatt aacatatata attcgtatac
     4021 tttggaaagg gttgttgcat caccgcatga aaaacggtga agatgtatcg ttagttattg
     4081 taacatctca aaagattgca aaaaatgtag ttcgtaatct taaagagaag aattatggca
     4141 tgtataaact tagtggtgtg attgtagtag acagagatat gtatggaact accatcggag
     4201 gtgtcccagt agttgcaaat gctgataatg cagcggaata tcttttacag aattgggtag
     4261 atgaagtatt catcaatgtt gacgaaagtg ttccatatcc caaagaatta atcgccagat
     4321 gttcggaaat gggacttgct gtacatatga atcttacaaa agtgtctgat agtacacgtg
     4381 gaaaacaatt tattggccga gttggtgatt atactgttat tacatctagt attaatgtta
     4441 tgacgatgag gcaagcattt ataaagagaa cgattgacat tcttgctgga attattggat
     4501 gtattgcaac aggtgttatt tttatcttcg tagcaccagt tatttacatc aaatcaccag
     4561 ggccgatttt ctttgcacaa gaacgtattg gtcaaaatgg taagacattt aagatgtata
     4621 agttccgtag tatgtatatg gatgcagaag aacgcaaagc agagttgatg aaagaaaata
     4681 agatgagtaa taatctcatg tttaagatgg attttgaccc aagaatcatt ggcaataaaa
     4741 tacttcctga tggaacaaag aagacaggaa ttggacaatt tattcgatct acatcattgg
     4801 atgagtttcc acaattttat aatgtcctaa tgggaaaaat gagcctggtg gggtttagac
     4861 cttgcttgaa gtcagaatat gagaagtatg attttcatca tcgtgcgaga attgcgatga
     4921 aacctgggat tactggaatg tggcaggtga gtggtagaag tgatatcaca gacttcgaag
     4981 aggttgtaaa acttgatgca gaatatatca ggaattggag tatgggatta gattttagaa
     5041 ttttgtttag gacagtcaaa actgtgcttc atagaaacgg ttccatgtaa gataaagata
     5101 ctatagaagg agtaaaacgt gatggcaaga aaaaaattaa aaatctgtat gctaggtcat
     5161 aaaagaattc catcaagaga aggtggaatt gagatagtgg tagaagaatt ggcaacgcga
     5221 atggttgcac ttgggcatga agtgacttgc tataatcgaa gtggtcatca tgttagcgga
     5281 aaggaatttg accagcataa aaataaagaa tataaaggtg taaaattaaa gactattttt
     5341 acattaaata taaaaggaat tgcagcaatg agttcttcag tatttggtgg aattagagca
     5401 gcattcggaa aatatgatgt tgttcatttt catgcagaag gaccatgtgc tatgttgtgg
     5461 ttgccaaaac tttttgggaa aagatgtgtt gcaacaattc atggtctaga tcatcaaaga
     5521 gaaaaatgga acaagcttgc tagtacatac atcatgttag gggaaaagtg tgctgtaaaa
     5581 tttgcagatg aaataattgt acttagtgaa agcgtccaaa attattttga agatatatat
     5641 ggaagaaaaa caaggtttat accaaatggc gttaaaaaaa ttgaaataaa atcagcagga
     5701 ttgattacag aaaagtatgg attaacaaaa gattcttata ttttgttttt ggggcgattg
     5761 gttcccgaaa aaggaattcg gtatctaatt gaggcattta aagatgttca gacagataaa
     5821 aaacttataa tagccggagg aagctctgat acagatgaat ttgcaaatga actaaaggaa
     5881 ttagcaaaag gtgatgagcg aattatattt actggatttg tacaaggaca ggaattagaa
     5941 gaattatata gtaatgcgta tatctataca cttccaagtg atttagaagg aatgccatta
     6001 agtttgttag aagcaatgag ttatgggaac tgttgtatag tttcaaatat ctcagagtgt
     6061 acggaagtag tagaagacaa ggctatgatt tttaagaaat cggatgtaag tgatttaaag
     6121 ataagattag aagaggcatg caatcaatcg gaaatggtta aagttttgaa gaatcaagct
     6181 actgagttta tttgtagtaa atataattgg gataaaatcg tacaagaaac attaaatttg
     6241 tataggggaa atatataatg attcgaatat tacagtgtgt gaatgatatg catcgtgcag
     6301 ggttggagac gatgctaatg aactattata ggaatataga tcgaactaaa attcagtttg
     6361 attttttgac acatcgtcca tataaaagtg attatgatga ggaaataata agtcttggag
     6421 gaaaggtcta ttatgcaccg agactttatc cacaaaatta tcctaaatat tttaaatgga
     6481 tggaacaatt ttttaaggat catccagaat atgagattgt tcattctcat attgatgcga
     6541 tgagttattt acctttaaaa gctgcaaaaa aagctgggat tccgatacgg atagcacata
     6601 gccataatac atcgatagat agagatttta aatatctttt aaagcaatat tatcgatgta
     6661 agttaacgtc tgttttgaca catgaatttt catgtggaaa agtagcaggt gattttttgt
     6721 ttcgaaatga taaattcact ttgattccaa atgcagtgga tgctaaaaaa ttttattatg
     6781 atgaaagtat tagaaatgat gtacgaatga aattaggaat ttcaagtcag acgtttgtaa
     6841 tgggacatat tggaagaatt tcatattcta aaaatcatcg ttttttgatt gaaattttta
     6901 aagaatttca cgctattaag aataattccg tattattaat tattggaact ggtgatatgg
     6961 aagaagaaat aaaaaattat gcaaagaaaa gtggcataga tgatgatatt aaatttttag
     7021 gaaatagaaa tgatatagaa aaattttatc aagcatttga cgtattaatg ttgccatcat
     7081 tatttgaagg ggttccgtta gtgggtattg aagctcaatt tgcagattta ccgtgtttct
     7141 tttcggagaa agttcctaca gaagttgctt tcagtgataa gaccaatttc ataagtttaa
     7201 atcaatcggc aaaagaatgg gcgagagaga ttgcaaatgt agatatctct catcgagatt
     7261 cagaacgttc gattttaata aaggcagatt ataatattca aacagcatac aaaatcttag
     7321 aatcaaaata ttatgagtta tttgaattaa tacaaagagg ttaaagaata tgacacagaa
     7381 acattcaagt ataaaaagaa ttatacatcg catatccgaa gaattgaatg caacaagacg
     7441 aattgcaaag ataacatctt ggaaggaagc atttgccacg ttacaagcta aaattgatat
     7501 tcaagtcatg aatcgtaatg gatataatga aagtaaaaaa cagaaggaac atttattaaa
     7561 aaaacatgag gtaatgctga agtattatga aaagactttt tcagattttt tgaaaacata
     7621 tgattatagt caaaatcaaa taattaatca atcaaaatca ccatattcta actgcatttg
     7681 ggtttgttgg tggcagggat tggatcaatc gccggaaatt gtaaaagctt gtgtaaaatc
     7741 aataaaacga aatgcaggga aacatactgt tataatttta acagaagata attatagaca
     7801 atatgttgat ataccaaaat gggtggaaga aaagaaaaac aaaggaatca ttacgagaac
     7861 aaattattct gatttattaa gattatcttt gctagcaaaa catggaggtt tgtggttaga
     7921 cgccacattt tattgtacaa aaccaatacc agatgagtat tttgatatcg atatttggtc
     7981 tattaggagg cctgattatg ctcacgcatc cgtagcatca ggatattttg cgggttattc
     8041 attagcatgt aatcaagagt ctagatggat gtttgctata attagagact tttttttaaa
     8101 ttattggaaa aataatgata tgatggtgga ttatttgatg atagattata tgattgtttt
     8161 agcacagaga ataaatcatg aaatagcaca gacatttgat aaaataccta gtaacaatcc
     8221 attatgtgac gaattgatta aaaatttaaa taatatatat gatgaaaaag tatggaactt
     8281 attaaagaaa gatactttac tgtttaagtt gacatggaaa caatctttta aaaagcaaaa
     8341 agatgataaa gttacttttt atgggatgtt ggtagaaaac aaattattat aaatattaga
     8401 ggtataaaaa gttatgaaac acaaggtaag tgtgatagta ccaatttaca atgttgaaaa
     8461 atatttaaga aaatgtgtgg atagtttatt gagtcagaca ttaaaagaga tagaaatcgt
     8521 attagttgat gatggttcgc cagatgcttc gggggaaata gcagatgagt atcaaaaaaa
     8581 atattctaat gtaaaaacaa tacatagaga aaatgggggg ttaggaccgg caagaaatac
     8641 aggaattgag aatgcgacag gagaatatgt ggcttttctt gattcagatg attgggtaga
     8701 aagtgatatg tatgaaaaat tatacttagt tgcgtccaaa gaaaatgctg atatagtagt
     8761 tagtggacat tgtgattttg cgaatgacaa agctatggtg aaaaagcaac atcctttgag
     8821 tgggaatgta tatgatacgc ctaatagtat taaaaatgta cgaaaaaatt tatttggaca
     8881 tagtccaaaa gatcaagaag tagaggcctt cccgatgtca gtatgtatgt ctttgtataa
     8941 taaaaatctg ctagataaat atagactaag atttgaaaaa attttatctg aagatacaat
     9001 ttttaacttg aatgcatatg atgtcgcaaa taaaattgct tttacagaat atacagatta
     9061 ttgttataga aaagaagaac aagagtctat tacaaaaagt ttatctccaa ataagcagac
     9121 ggaatttaaa gaatttttaa taactctaaa agctatagca tcgaaagaag atttagaatg
     9181 tcaaatcaga gcaaaacgta tggcaattga ttattgtagg ctttatgtgg gcttagttga
     9241 tgatgcaaat ttgggaatta aagagaaaag aaagtatata aaaaaattta ttgaagatga
     9301 gggattaatt caatgttgga agggatatcc tacaaatacg ctaccatttc aacaaaaatt
     9361 ttttcataaa atgcttgaat gtaagtgtta tacaatattg ttgttgttaa gtagaattag
     9421 aaagataatt aaaaatatga ccagaaaaaa attgtttata atattataaa ctaaaagtat
     9481 atcaaataat agaaattaac aagttacaaa aaaatattat aaagaataat attttc
//
LOCUS       DACDTH010000092         9362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDTH010000092 DACDTH010000000
VERSION     DACDTH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.095"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(93..761)
                     /locus_tag="IWK54_002560"
     CDS             complement(93..761)
                     /locus_tag="IWK54_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9594638.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="IWK54_002561"
     CDS             895..1182
                     /locus_tag="IWK54_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594639.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1598..3136
                     /locus_tag="IWK54_002562"
     CDS             1598..3136
                     /locus_tag="IWK54_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9594640.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3133..4098
                     /locus_tag="IWK54_002563"
     CDS             3133..4098
                     /locus_tag="IWK54_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9594641.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4085..5407
                     /locus_tag="IWK54_002564"
     CDS             4085..5407
                     /locus_tag="IWK54_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9594642.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5470..5574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5648..6391
                     /locus_tag="IWK54_002565"
     CDS             5648..6391
                     /locus_tag="IWK54_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9594643.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6406..7398
                     /locus_tag="IWK54_002566"
     CDS             6406..7398
                     /locus_tag="IWK54_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9594644.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7410..8147
                     /locus_tag="IWK54_002567"
     CDS             7410..8147
                     /locus_tag="IWK54_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9594645.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8137..8859
                     /locus_tag="IWK54_002568"
     CDS             8137..8859
                     /locus_tag="IWK54_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9594646.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8943..>9362)
                     /locus_tag="IWK54_002569"
     CDS             complement(8943..>9362)
                     /locus_tag="IWK54_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9594647.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3069 a         1607 c         1972 g         2714 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     1501 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     1561 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     1621 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     1681 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     1741 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     1801 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     1861 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     1921 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     1981 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     2041 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     2101 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     2161 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     2221 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     2281 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     2341 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     2401 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     2461 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     2521 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     2581 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     2641 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     2701 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     2761 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     2821 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     2881 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     2941 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     3001 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     3061 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     3121 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     3181 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     3241 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     3301 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     3361 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     3421 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     3481 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     3541 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     3601 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     3661 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     3721 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     3781 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     3841 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     3901 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     3961 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     4021 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     4081 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     4141 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     4201 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     4261 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     4321 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     4381 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     4441 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     4501 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     4561 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     4621 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     4681 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     4741 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     4801 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     4861 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     4921 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     4981 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     5041 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     5101 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     5161 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     5221 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     5281 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     5341 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     5401 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     5461 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     5521 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     5581 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     5641 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     5701 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     5761 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     5821 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     5881 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     5941 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     6001 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     6061 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     6121 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     6181 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     6241 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     6301 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     6361 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     6421 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     6481 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     6541 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     6601 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     6661 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     6721 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     6781 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     6841 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     6901 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     6961 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     7021 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     7081 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     7141 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     7201 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     7261 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     7321 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     7381 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     7441 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     7501 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     7561 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     7621 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     7681 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     7741 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     7801 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     7861 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     7921 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     7981 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     8041 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     8101 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     8161 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     8221 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     8281 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     8341 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     8401 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     8461 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     8521 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     8581 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     8641 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     8701 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     8761 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     8821 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     8881 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
     8941 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
     9001 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
     9061 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
     9121 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
     9181 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
     9241 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
     9301 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
     9361 ta
//
LOCUS       DACDTH010000093         9168 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDTH010000093 DACDTH010000000
VERSION     DACDTH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.096"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            355..1590
                     /locus_tag="IWK54_002570"
     CDS             355..1590
                     /locus_tag="IWK54_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594648.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            1674..2762
                     /locus_tag="IWK54_002571"
     CDS             1674..2762
                     /locus_tag="IWK54_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594649.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            2759..4180
                     /locus_tag="IWK54_002572"
     CDS             2759..4180
                     /locus_tag="IWK54_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9594650.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            4273..4932
                     /locus_tag="IWK54_002573"
     CDS             4273..4932
                     /locus_tag="IWK54_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9594651.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            4951..6534
                     /locus_tag="IWK54_002574"
     CDS             4951..6534
                     /locus_tag="IWK54_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAP9594652.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            6669..7247
                     /locus_tag="IWK54_002575"
     CDS             6669..7247
                     /locus_tag="IWK54_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594653.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            7193..7711
                     /locus_tag="IWK54_002576"
     CDS             7193..7711
                     /locus_tag="IWK54_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594654.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            7719..8759
                     /locus_tag="IWK54_002577"
     CDS             7719..8759
                     /locus_tag="IWK54_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9594655.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            9008..>9168
                     /locus_tag="IWK54_002578"
     CDS             9008..>9168
                     /locus_tag="IWK54_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9594656.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         3324 a         1017 c         1706 g         3121 t
ORIGIN      
        1 agaagatgtg cttctagtca tgtgatggga gaaaataatt taactgttag agtgaatagt
       61 taaattattc tggttatatg aaaaaagtag tttctaaaat aatcttgcag ggattaactt
      121 aaagaaattg agagaatagt ttctttgaaa tttctatata caatgagaag ctgaaattta
      181 cttttatttg atgactggaa atagaaatta aatatattgt attgttacat taaagaaaac
      241 ttgtatggaa aaatcagtac aataccatta tttaatgttc ttcacttaaa gcgcaaaaaa
      301 tatcagttta ttaaactcgt atatagaaca aatatatgta gacggggcga aacgatgagt
      361 gtaaaaaaaa tgctatttat ggtaagtaca ataattgtat taacatcccg attttgggga
      421 attaatacta gtttatctat gcgatatttt attatggcaa tatgggattt atattttatg
      481 ttaagctatt tttcttatcg tgagaaaaca tataaaaaat acgttatcaa gaataacttt
      541 tggttgacta taatgccgtt tgtgatattt tctatctata ctctaataat atggggaatt
      601 ggttcaaatg cagaatttga aaattttata aagttatgtt ccacgttgct ttatttaatt
      661 cttgcatatg gttacgcatg tacagcattt tacttattta aggcagaagg aattgatttt
      721 attttttggt caggtgttat ttcatatatg agtggttcag ttttttattt aattgtatct
      781 tatggtttaa atggtttaat ttcttatata aaaagtctta taacaggtga aacaaatact
      841 gcaaattttg ctatggaggt acatgattta acttttgcga tgggggtttt tttcctttat
      901 tacgtatttt ttgaaaataa aaatacaaaa aaacataaga taaaaattat cgtatcttta
      961 attttaattt tcatgggatt gaagagaatt gaactgttag ctttaagtgg tgcaattcta
     1021 gtgtattatg ttttattaaa gtggggaaaa aagattcaaa tccgtgcatt agtgtgtact
     1081 atttgtgcaa ctgttatttc gttagtgttt atttacatta tagatacagg aattttggca
     1141 ttgcttatgt caaatttggg cattacagat gacggtgcga ggctttcatt ttatctatat
     1201 tcatctaaat tttatgaact tggtttagga tatgtaggaa caggatggac atggttttca
     1261 aaatactttt ttaatcttta tttatcgcac tttaggttaa atggtggaag aattgcagca
     1321 agcctacaca gtgatatatt aactatttat atagaagttg gatgtatagt ttttattata
     1381 tggatgtttt ggttattttt tgtgaaaccg ataagactaa ctaaaaaatt tggattgaaa
     1441 acaggggagt gtgtattatt attaactata tatatgttta tattatattt gacggataat
     1501 acgctcactt atccagacac tcaaatgatt tttctcttag tacctatgat agtttcgtgg
     1561 gttgataatc aacaacgaat atcggaatag atagctaaag tatattttaa ttatgaattt
     1621 ataattagtt gtaaaagtta atatttaacg gacaggaagt agggtgaaaa aatttgaaaa
     1681 aaattttagt gttagcaaat acatattatc aaataatagt agcaatacaa atgaaaaata
     1741 caattttttc cgatgagtat gttgaaatta ttatttctga tcattctaaa aacactaaag
     1801 aaattgcgaa aaaaatagaa gaaaaaaaag tgtttgatgg ctgtcattat ttagaaagca
     1861 aatcttttga tgataatatt gggtattttc aaaagattaa ggactgtctt cagatttctt
     1921 gtggtaaatc aaatcgatat gcctattatt tgaaaaactg taaaagtgaa tattttgatg
     1981 aatttatttg tttttcaatg ggagaggttt ggttggaagg aatatatgct tgcttatatc
     2041 gaaataataa aaatgtacag gtatcaattt tagaagaagg tattttaagc tatggagttg
     2101 acattattcc aacagcaaga acaaaagcta tcgcagtact caattactta aaaaaaagga
     2161 ctccactata taacaaaagc tataattttt attgtttttt tcctaatata tatacaggaa
     2221 gagagaatac aatacaaatt ccattagtta cagctgggtc aagaaccgca ttgcaacttt
     2281 cggatatttt taaccctatg atagatattt ataaagaaaa aaaatatata ttttttacgt
     2341 ctgtttatga ttttgagggc ggagaacctg tcggagaata tgaattagta tgcaaaatag
     2401 cagacttagt aggaaaagat aatctaattg ttaagacgca tcctagagat gtaagaacaa
     2461 tttataaaga taatggattt atcgaggata aaaattcatc aataccttgg gaggcgattc
     2521 aattgtcagg cgattttaac gataaaatat ttatgacaat taattcagga agtgttattt
     2581 caggtagtac aatgtctgca aaacctgtac gcacgtatta tatgtataaa ttgtgtaaca
     2641 tagaaggaaa tccgtcttgc aaaaagaatg cgtatgatat tgagcgattg cttaagcaaa
     2701 aaacgatgaa aaacatttta aagaatgtga aaatagcaga aagcttagat gatatactat
     2761 gagcaaaact aaatatttaa ttaaaaatat gggaatatta acaataagta atttttcttc
     2821 aaaaatttta gtttttttgt tagtcccttt atatacaagt gtattatcca caacagaata
     2881 tggcacatat gatttgacaa tttcaacagc cacattattg tttccaattt taaccttaaa
     2941 cattgtagat gctgtgatgc gctttttaat ggataaagat aaaaataaaa aaaatatagc
     3001 atctatagcg attaaagtta tatttttaag cgtgatagtc tttggatccg taatgtattt
     3061 gttaaatatt acaaaattat ggcaagatgt ttatggatta gaaaaatata tttttttgta
     3121 ttatatatcc tatgtatgga atcaatttct aatacaattt gctaaagggt tagaaaaagt
     3181 taaagctatg ggcatagcag gtattatcag cacaattgta acaataattg ccaatatttt
     3241 cttcttgctt attctgaaat ggggattaat tggttttttc attgcaaata ttttatcaca
     3301 gattatttca gctacttatc tgtcagtatt agtgagaata tgggaatata tcgattttga
     3361 ggctaaagat cctcagctaa agagagaaat gctaaattac tcgattccat taattgcaac
     3421 tgttgttgga tggtggatta atagtacagc tgataggtat gtggtttcct ttatagtagg
     3481 tgtctcggcg aatggcttat tgtctgtggc atataaaata cctcaaataa ttaatacttt
     3541 gcagggaata tttatacagg cctggcaaat ttctgcaatt aaagaatatg gagataatga
     3601 gactgcaaaa ttttatggaa atacatttgt tatgattaat ttgttaatgt gcataacctg
     3661 ttcaagtctt atcatattaa ctagaccgtt ttcacatatt ctttatgcga aagactttta
     3721 tgaagcgtgg aaatatgttc catttttatt aatagctaat gttttaaatt gtgcatcagg
     3781 tctgttagga cctatcttat cagcaaaaaa agattctaaa tccatgatgt ggtctgcaat
     3841 cataggggca ttagttaatg tgattatgag tgtagtcctt gtttataaga taggggttca
     3901 aggtgccaca atagccactg ttatatgttc atatgtaata tatgttgttc gtaaacgagc
     3961 tgtgggaaaa gatattttta taaagcatta tcatattgtt gtggaatctt ggggattatt
     4021 gtttgttcag gcaatcatag aaatatattt taaaaattat gtaatagaaa ttataattat
     4081 gattatttta attattataa atatcagtta tattaagcag ttgtgttata gaggaagaaa
     4141 cattatttca atgattaaga gtcgagagca caaacggtag acgatgttat ttttataaaa
     4201 agatataatt tattaatggt cgataattat gaaaggaaaa atattcttaa aaaaaagagt
     4261 ataggatgtc gtatgaaaat agtcgctata gtaccaatga agttaaataa caaaagattg
     4321 ccacaaaaaa acacgaaagc ttttaccaat ggaaaaccat tgtgttatta tatactttca
     4381 acacttttaa aagtaaaaca gattgataaa gtgtatgtct attgtagtaa cccagatatt
     4441 aaagaattta taccagaagg tgtaacattt ttaaagagat ctagttcctt agatcaggat
     4501 acaacgagta ttacacaggt attagccggt ttcacaaaag aagtagaggc tgatgtatat
     4561 gtaatgacac acacaacagc accatttatt agcgaaaaat ctattgaaaa aggactagat
     4621 gccgtgctga caggagaata tgattctgca tttgctgcaa aaaaattgca agatttctta
     4681 tggaaagacg gtgaaccatt taattatgaa ttggacaata ttcctagaac acaggatttg
     4741 ccgcctttat acgaagaaac tagtggattt tatatttata atcgcaatgt gatgaccgag
     4801 ttgcatagaa gaattgggaa aaaaccttgc atagttgaag taggtgagat tgagtcagtt
     4861 gatattgatg aagccgagga ttttatgatt gctgatgcga tttataatca tcttatttta
     4921 caaggtagtt aagacagggg gaaggttagg atgaacaatg tacagatttt agattgcaca
     4981 cttagagatg ggggctattg taacaattgg gaatttggat ttgataatac aaaaaaaatt
     5041 atttctggac ttatagaatc aggaattgag attatagaat gtgggtttct cacaaataaa
     5101 acagtataca ataaaaatgt tacaaaatat acgaccttag aagaattagc acagataatt
     5161 cctgaaaaaa gagaaggaaa attatttgta gcaatgatga actatggcga atataatata
     5221 gaggatcttc cagattatga tggttcatct attgatggaa ttaggattgc gtttcataaa
     5281 aaaaattgca atgaagcgtt gaaattatgt cgtgagataa agaaaaaagg ctacttagtt
     5341 tttgttcaag ctatggtatc tctcaattat acagatgaag agtttttagc agtaataaaa
     5401 aatgtgaatc agtttaaacc atacgctttt tatattgttg atagttttgg aatgatgaaa
     5461 ggaaaagatt taactcgtct tttttacatg attgagcata atctagaaaa agatatacag
     5521 ataggttttc actcacataa taacatgcag cttgcatatt ctaatgcaca aaaacttata
     5581 accgtacaaa caaatcgaag cctgattatt gattcttcgg tgtacggaat gggaagagga
     5641 gcaggtaatc ttaacacaga actatttgta gaatatttga atgataatgc aggaaaaaaa
     5701 tatcaattaa aaccattatt atatataatt gatgaaatac taaatagttt ctatcagaag
     5761 agttattggg gatattcatt accaaactat atttcagcag ttcataatgc tcatcctaat
     5821 tatgcaagct atctgtcata taagaatact ttaacaattg aagcaatgaa tgaaatattc
     5881 gaaatgatgg atgaagaaaa aaaggtatct tatgataaac aatatataga agatttatac
     5941 tacaaatata tggaaactgg gaaaatacag gaggaatata tggaagaact gagaaaattt
     6001 ctctcagaca aaaaaattct actgattgca ccaggaaaaa gttctgtaga tgaggtagaa
     6061 agaataaaag agtttgttac caaagaagaa gtcgtaataa ttagtgtaaa ttttgaatat
     6121 tctcatatag atatagatta cgtattttta agtaatttaa gacgttttca tgaattaccg
     6181 aaagaaagca gaactaaatg tattgttaca tcaaatatac aagcatcgga tgtatattta
     6241 cggacgagtt ataaaaagtt gttatctgaa agggaagttg taagagacaa tgcaggatta
     6301 atggcaatta gatttttagc cgatatgaat gtggatgaaa tattcttagc tggatttgat
     6361 ggatattcac ataatgaaga tgagaattat ggtgagcaat ccatggaaat tataacaaaa
     6421 tttgcattat tagatgcaat gaacgtgcaa atgagagatg aattgtcaga attagctaaa
     6481 aaagtaaagg tgacattttt aacggatcca cgttgggttc gaattgataa gtagtagtaa
     6541 taatgttttt aaaattttga taaagagata gtgagagaat aaaaatgggt aaagtcttaa
     6601 ataaaaaaga agaaaaatat ttaagggacg atggggcgag atgaaactct aattttgaac
     6661 ttttaagaat catttctata ttgtgtattg ttgcttacca tgctaatttg cgtttaaatt
     6721 ataatgcaga cggaattata gtacatggtt cattaactgc taaccagatt ttcttgaatt
     6781 atattggtat ttggggtcag ctaggagtta attgttttat atttatttcg gcatattttt
     6841 taagtgagaa aagaaacatt cactttgaaa atgttttggg aattgtatta gaatgtattt
     6901 tttatagtgt attagtttat ggaattcttt gtgcatgtgg aatagaaaca ttttcattga
     6961 acagctttgt aaaagaaata ttatcatttg ctctaggtag atattggttt attacatcct
     7021 atttagcatt gtatattcta agtccgtatt taaatatcat gcttaacaat ctggaaaaac
     7081 ttcagttgca aaaaataagt atgttattgt ttataggtgt atgctgttat cgtatgttat
     7141 ttaaaacatc tttgtttggg gattgtgctg ttttaattgc actatatgtc atatgttata
     7201 cactgaaaaa aacgataata atatttttga gagacacaga atcataggaa caattgtatg
     7261 tataacagtt ataccacttc tcatatccat aggaatactt ttcaaattta taccaaatgc
     7321 aatggttaac cagatggttc atattggttc tccattgatg gttatagcaa gtatattttt
     7381 attttatgta tttaaaaatt tttcatttta cagtaagtat ataaataaac ttgcgagaac
     7441 cagtttaggg gtatatattt tacatgaaaa tcattatttc attgatggta tgaagtctag
     7501 aggcttaata tgggatggga tatttggatt aggcaaaagg tttttagata gggatttttg
     7561 gatttggttt ataattgcag ttgtagttgt gtgcataatt tgcatggcta tcgatgttgt
     7621 aagagaaaaa tttgaaagac agatttttaa aattaagagt gtaaagaacc tgtgtaaact
     7681 tatgaatcat gttataaata tatacccata attaataaat gaggagaaaa gaagtgaaag
     7741 ttatatcatt cggagaatta ttattgagac ttgcagcacc cggttattca aagttatttc
     7801 aaagagatag tttggagact acattctgcg gaggggaagc taatgtagcg gtatccttag
     7861 caatatttgg atatcaatca gaatttataa caaagcttcc ggattcagat gttggatatg
     7921 cggcagctaa tgctttgcgt tattttggag taaatgttga taatattata tttggagagg
     7981 gacgcatggg actgtattat ttagaaaaag gagcagctca gagaccatct aaagtaattt
     8041 atgacagaaa attttctgca atttcgttag ctgatagtga tgaatttaag tggaaggaga
     8101 tcctaaacga tgcaaattgg tttcattgga cagggataaa tccagcattg tctgagaatc
     8161 tctttgagac gtccttagat gcatgtcagg ctgccaaaaa aagaggtata acaatttctt
     8221 gtgatttaaa ttatcgatcc aagctttgga catcagagga agcaaaagaa aagatggaac
     8281 agatactacc attggttgat atttgtattt gtaatgaaga agatgcagaa aaagtgctag
     8341 gaattaaagc tgagcataca aatattaata gtggagaatt aaataaagat ggctatgtgc
     8401 aggtggctag ggagattcac aagaagtttg gatgtaggta tgtagctaca actctgcgta
     8461 gaagttattc ggcaagtaga aatggttgga gtgcaatgtt atacgaagca gaaaaagata
     8521 aagcgtattt ttccacagaa tacaacatac aaattgttga tcgcgttggt ggtggagaca
     8581 gcttttctgc tggtataata tatgcgttat gtgaaaatat gtccggacaa aaggctattg
     8641 aatttgcgac tgcatctagt tgtttaaaac atactataga aggtgatttt aatcgaacta
     8701 caattgagga tgtatataat ttaatgaata gcggtggaaa tggtcgcgtg ttgcgttagt
     8761 caacatataa agataaatca cagtaatttt tatggtgatt atctgtctta gtagtgatta
     8821 gaataacgtg aaaataaatt gttgagcgtt tatactgttt tgaaaaataa atttaacata
     8881 ttgaaaaata caaatatata cgtatattat aaagcgccga ttgtaaaatt aagctagaca
     8941 actaaaacag tcatttgtgc tacactcaaa atagttccga ccaaagaatt ataaggagtg
     9001 agtgcaaata acctatacct atcttacaac ggatgaactt gtaataatag agtcttattt
     9061 gaaaattaac caatctgttg ctaaaactgc gcattgcttg aatcgttcaa gtcaaacgat
     9121 ccataaagta tatctgttct tcaagcaaag aaaatcagcc ttagaata
//
LOCUS       DACDTH010000094         9021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDTH010000094 DACDTH010000000
VERSION     DACDTH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.097"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            126..2915
                     /locus_tag="IWK54_002579"
     CDS             126..2915
                     /locus_tag="IWK54_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9594657.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            2962..3489
                     /locus_tag="IWK54_002580"
     CDS             2962..3489
                     /locus_tag="IWK54_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9594658.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3510..4700
                     /locus_tag="IWK54_002581"
     CDS             3510..4700
                     /locus_tag="IWK54_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9594659.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            4740..6038
                     /gene="asnS"
                     /locus_tag="IWK54_002582"
     CDS             4740..6038
                     /gene="asnS"
                     /locus_tag="IWK54_002582"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9594660.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7214..7888
                     /locus_tag="IWK54_002583"
     CDS             7214..7888
                     /locus_tag="IWK54_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9594661.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            7885..8226
                     /locus_tag="IWK54_002584"
     CDS             7885..8226
                     /locus_tag="IWK54_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594662.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            8256..8615
                     /locus_tag="IWK54_002585"
     CDS             8256..8615
                     /locus_tag="IWK54_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9594663.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            8819..8998
                     /locus_tag="IWK54_002586"
                     /pseudo
     CDS             8819..8998
                     /locus_tag="IWK54_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
BASE COUNT         3005 a         1623 c         1963 g         2430 t
ORIGIN      
        1 acgcccagac atttgcgtgt ctgggcgttt ttgtctattt tctttaaaaa gcgatgagag
       61 tatttttctg cactttgtag tataatttca aaagaacatt agaggcagga gaagaggaag
      121 atattttgaa accaagaact tatgcagtcg ttgatatcga aacgactggg acgaatccaa
      181 aagaagatcg gatcatccaa tttggctgcg tcatgattga atcaggtcgg atcacttcac
      241 gtttttcgat cgatattcat ccagggcgga agatttcaaa acaaatccag catttaacag
      301 ggatcacgaa tcaacgcgtc caaaaagcgc cttatttcga agatgtcgcc caaacgattt
      361 acaatctttt ggcagacacc atatttgtcg cgcataatat ttattttgat tataattttt
      421 tgaaccatga actcatgcga tgcggacttc ctagtttgaa gataccaggc atcgatacag
      481 tagaacttgc acaaattttc ttgccgacgg aaccaagctt tcgcttagct gatttgtcag
      541 aaagccttgg atttattcat gagaatcctc atcaggcaga tagtgatgcc gaagtgacag
      601 gtcaacttct tttgctgatc gaagaacgga tgagaaaatt gccaatcatc acgttagagc
      661 agattgcgcg attaagtaga cacacaggta tggatacgag tcgtttcatt tatcatgtga
      721 ttgaagaaat gaaagaaaaa ccagagccac ttgatccctc attagaaatc gtcgatgggc
      781 tagctttgca gaaaaaagaa gtggaactat tcacatccgt ccattatggc gagcggacat
      841 atccaagaaa aaaacaagca aaagaaaaaa tgtttggtaa aacactgatg tatcgaaaag
      901 aacagaacag actgatgaat gcggtctacg accatttcac aaaagatgaa tcaaaagatt
      961 tgatgatcga agcagctaca ggaatgggta aaacaatcgg ttatctgctg ccattgagtt
     1021 atttggctac acctgaaaaa cccgctgtca tcagtaccgt ttcgctagtt ttgcagcagc
     1081 agatcataga aaaagacatt ccattgctga atcaattgct agatcagccc atacaacctg
     1141 taattatcaa aagctatcgc cactatattg atttgcagcg ttttaaaggc acacttgtgg
     1201 aaccgccaga acaaaaacaa tatgctttgt atcaaatggc ggtcttagtt tggctgacgc
     1261 aaacgaagac tggtgatttg gatgaattac atttaacaaa tttgaaccat tcattctttg
     1321 cagatatcgc gcacagaggt attggatttt tagcaagaaa tcagtcattt tacgaacaag
     1381 actttgttcg tttcttgcaa aagaaaatca ggcaaagtaa ttttttgatt gtcaatcatg
     1441 cgtttttagt acaagaaaca cagcgaaaag aaccattgct tccagctagt ccatttttgt
     1501 tgatcgatga agtacatcat ttgccggaaa tcgctgaaaa agtaaatgac aaacgattaa
     1561 gtattaacta tttccataaa caaatccagc aattagaaga accaaatcag ctgtttgacc
     1621 gaatacaaag ctatttaccg aaagaacatg aagccttacg ctggttatcg atttatcgag
     1681 aagaactttc tgcgctatgc gaagcacaga tttgtatggc agaaggatta atgagtttag
     1741 tgagcggcaa gcaacctaac ggacagtatc cagaagaagt catgatgaca aaagagatgt
     1801 tggatcagct gccactggaa tctgggacag ccattgatca cctgcagctt ttttatcagg
     1861 agatccagca gctacagtca caaatgcgtc tgacttttga gattatccaa gaaagctgga
     1921 caaaccagat gcggatcgat ttagcaagtt tatatagcct atttgatcaa ttagacaagc
     1981 aatacttgct gatggatcaa tggctggaag actggcgaga tcatttgatc cattggttcg
     2041 ttcctgggaa cacagcgcag cagatgattt ttcatctcca tgattttcaa gcggctatgt
     2101 tgccgtcgac tgtttggtat cctcgatatg atcgtattct ttatattgga ggaacgctga
     2161 agatcggccc taatcgacac tatctctcta agaggcttgg cattgaagca gcacctctta
     2221 aagtgattgc ctctccttat gattatgaga aacaggcaag gatctatgtg ccaaaagagg
     2281 cacctgccgt ccatcaagtc agcaatcaag catatatcga ttatttagag acagcactta
     2341 caggtctgat cgaacaagaa gatcggccaa tacttgtgtt attcacttct catgatgttt
     2401 tgcagaaggt ctatcagaaa atgcatctgt ctatgctaga aaaaggacga gaaatactag
     2461 ctcaagggtt tggtggaagc cgggagaagc ttttgaaacg cttcattttg tcagaaaaaa
     2521 gcgtattgtt tggcgctgac agcttttggg aaggtgtaga tttgcctgga gatgctttgc
     2581 agatcgttgt agtcacacgt ttacccttcg ataatccgct tcgcccgatg gtcaaagcta
     2641 ggaacgctta tttagaatca gaggggatca atccttttta ccaggaatcc gtacctaaag
     2701 cagctttgaa gttacgccaa gcattaggtc gcttgataag gacggagaca gatcgtggcg
     2761 tattgcttat tttggatcga agactagtca ctgcaaaata tggcgcacgg atcatccatg
     2821 cattaccgaa aaaagtaccg atcaaagagc tagctatgga ggaaatctat caggatatcc
     2881 atgaattctt aaagaaaaat gaagaaggaa gttagaggag ctgtcttttt tcataattaa
     2941 aatctgctat aatgaaaagg aatgaagata aaggagcgac gacccttgaa gaaaaaacag
     3001 acaaaggaaa aaacgattaa tcgcgtgtta cttggcttga tcgtattttt attagcggtc
     3061 atcgtgatca gctcgatttt ttacttgcgt tccagtcgtc cggcggtgca ggcgaagaaa
     3121 gaagcagtag aaatcgcaaa gaaatatgca aatcttgagg aaaccgatca tttttattgg
     3181 tttactcgaa agaagacata cttttcactg acaggtacaa atgataaagg aaaagaaatc
     3241 gccgttatca ttcctaaatc tggtgaaaaa gtaaaagtat tggatcaaac aaagggactg
     3301 actgaaaacg aagcaaaaca gacaattgct gcggcccatc ctgaaatcca aatcgaaaaa
     3361 gctgctttag ggatgtatga tgatcagcct gtgtgggaag tagtaggaaa agaaacaaat
     3421 gggaaactga attattacct gattggcttt gaatctggag atgaagtcaa gacaatcaag
     3481 ggaatttaaa aggtaaagga atgaatggga tggagttatc aaaaagaaca aaacgattag
     3541 aaccgtctgt cactttggca gctgcggcaa aggccaaggc attaaaagca aaaggcagag
     3601 atgtgctgag tttgactgtg ggagaaccgg attttgcgac accagaaaac atccaagaag
     3661 cagcaatcga agcaatccgc aatgggaaag ctagctatta tacaccaaca gcaggaatcc
     3721 cagagcttcg acaggcaatc gttgattatc tgaagaaaaa ctatggactt gtctatgaac
     3781 cttcacaaac agtcgtaaca gacggagcga aatttgcttt gtataccctt ttccaaacga
     3841 tccttgaccc gcaagatgaa gtcatcattc ctgtgcctta ctgggtcagt tacggtgagc
     3901 aagtaaaact ggcagaagga cttcctgtct ttgtgaaagg ggaagaatcc aatagctgga
     3961 aagttactgt tgaacagcta gaacaagcac gttcagaaaa aacaaaagcc gtaatcatca
     4021 attctccatc gaatccgact ggaatgattt atagcaagaa tgaactgcag gcaatcggtg
     4081 aatgggcagt aaaaaatgat attttgatag ttgctgatga tatttatggc cgtttagttt
     4141 ataatggcaa tgagtttact ccaattgcaa cgatttcaga agcaatcaaa aatcagacca
     4201 tcatcatcaa cggcgtgtct aaaacatatg cgatgacagg ctggcgcatt ggttatgcgg
     4261 tgggaaatcc tgtgatcatc aatggaatga tcgctgttgc ttcccaatca actagcaatc
     4321 cgacagcagt cagtcaatat gcagctgttg aagcattgaa aggtgagcaa gatacagtag
     4381 aagaaatgcg gaacgccttt gaagaacgcc tgaatacgtt atatccgctt gtggcagaac
     4441 tgccgggtgt ttctcttgaa aagccgcaag gagctttcta tctttttcct aatgtaaaag
     4501 aaacgctacg catgtgtaag tatgaaaatg tcacacaatg ggtagaagat cttttagaag
     4561 aaacaggggt agcgcttgtg actggcgaag gctttggggc accagaaaat gtccgcatga
     4621 gttatgccac tgatcgcgct acgttagaag aagcggtacg cagaatcaag caatttattg
     4681 agagtaaaag tcaaaattaa tcctgaagaa attcaaggaa tataaaagga gagtcatacg
     4741 tggaacaaat tcaaatcatc gattcgaaaa accatgtggg agaaacagta acaatcggcg
     4801 cttgggtcgc aaataagcga tcaagcggaa aaatcgcatt tttacaatta agagacggaa
     4861 cagccttttt ccaaggtgta gttgtaaaaa gcgaagtgtc ggaagaagtt ttccaaacag
     4921 ctaaaaatct acaccaagaa acatccgtgt tagtaactgg agaaatccgt gaagacagtc
     4981 gttcaaaatt tggttatgaa atcggaatta caaatgtgga agtggtaagc gagagccacg
     5041 attatccgat cacaccaaaa gaacacggaa cagacttctt gatggatcat cgtcatttat
     5101 ggctgcgttc ttcaagacaa catgcgatta tgcagatcag aaatgaaatc attcgtgcaa
     5161 cgtatgaatt ctttaatcaa catcattttg tcaaaattga tccgccgatc ttgactggtt
     5221 ctgcaccaga aggcactacg gatctattcg aaacaaatta ctttgatcaa aaagcttatt
     5281 tatctcaaag tggccaatta tacatggaag ctgctgctat ggctttcgga aaagtctttt
     5341 cattcggtcc gactttccgt gctgaaaaat caaaaacacg acgtcatttg atcgaattct
     5401 ggatgatcga accagaaatg gctttcactc atcaagaaga aagtttagaa gtacaggaac
     5461 aatatgtagc attcttagta caaaatgttt tagatcattg cgaatatgca ttagatgtac
     5521 taggacgaga caaagaagtc ttaaaaactt atacaaaatt gccattccct cgtattactt
     5581 acgatgatgc aatcgaatta ctgaagaaaa atggatttga tgacatcgaa tggggcgaag
     5641 actttggttc acctcatgaa acgttcattg cgaattcatt cgaacagcct gtctttatcc
     5701 taaactatcc aaaagccatc aagccattct acatgaaacc acatccaaca agagaagacg
     5761 tggtaatttg tgcagatatg atcgcaccag aaggttacgg tgaaattatt ggcggaagcg
     5821 aacgggcaac agactatgat ttcttgctag aacaaatccg taatcacggt ttggatgaaa
     5881 aagaatattc atggtatctt gatctacgta aatacggttc tgttccgcac tctggttttg
     5941 gtctaggcct tgaacgaaca gtcacttgga ttgctggtat cgaccatgta cgtgaagcta
     6001 ttccattccc acgtttgttg aaccgaattt atccataaga ggtgatgaaa aagctgttct
     6061 gcatcaagta attagaacag ccaagcaaaa acagctgttc atgtttttga attggcggga
     6121 cttattaccg cagatgtaag cttttgaata ccgtttagtg gagaggttgt gaaaaagctg
     6181 tttagtttcg agtattaaga aaaattttgt ggaaatggct ttccacattt ttgcataaat
     6241 ttggcttaat accgagaaac tggcttttga acaccgttta aggaagaggt tgtgaaatca
     6301 gtgccttgac ctgagtatta gagaaacaaa acggaaaaat agcttctcat attttaccgt
     6361 ttttttagct aatatcgagg gtcagctgat tgaacaccgt ttatatcaag aggttgtgcg
     6421 cctgccgttc agctccgagt cacaaggagt aatttcaaaa aacagttttt catgtttgat
     6481 gactattgtg acttgtgacc gaggagttgg ccggagagca acgtttattt aaaaaaaaga
     6541 actgcgaact ctcgcagttc tttttttaaa atacgggata aaggaagttt ccagcagaca
     6601 agagaagaaa gcaaaagaaa ttgctatttg attcacaagg ttctataacg aaatcgcaat
     6661 caatcaaaca tatacaaaga aggttattca aatggaaatc tgatttgatg atgcttctgc
     6721 tgttgaaata aagcagcatc tagtacctga aaacaaacta atagtaacat tcgaagatgg
     6781 agtaggtccg tattttcagc atgcaatgat ccatatgtaa gtttaattca tccttgaaat
     6841 catcggacca gataaagtta tgaccaatct atcccaagca atctagaaga gatattagta
     6901 aaagaacatt tactggaagt cttagatgag cctataaata tatgttctga tggcgtcatc
     6961 agtggtctga ttgacggaag cttaggtgtg atcgatttct ctggaccaaa caacgaagga
     7021 atcaagggga atccggcacg ttaagaaaaa aagtataaaa aactagacac ttgatgagcg
     7081 ttcattcaat tgattgaagg gactaggctg gtgtcatttt ttatacatat attttctctt
     7141 tcgtcttgat agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag
     7201 gagtttttga caaatgaaaa aacgtatatt atttatcggt gacagcatta cagacgcagg
     7261 aagagaccga gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa
     7321 actaatctcc caatatccaa cgatgaaatt tgaatgcatg aaccgtggga taggaggaga
     7381 caaaattgca gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta
     7441 tgtatcgatt ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt
     7501 cggaactgag aaatctgcgc agcaatttga atccgtttat cgaaaattac taaatgaact
     7561 tcaaacagcg gaaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga
     7621 cgaccgtcat ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc
     7681 taaggaatat ggcgcattgc tggttccttt agatggcata ttcaatgctt tagggattgc
     7741 ttatggtttt tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga
     7801 gatcattgca caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa
     7861 aaatgagcga aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagattta
     7921 cctcaaatca atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca
     7981 gctgacctaa atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt
     8041 atcggatttg cgcaagcgga cgtgcaacag tcagaagaac gggtggatca gcgaccatca
     8101 ggaaaactaa tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa
     8161 tttgcagaat acttgatgct ggaagctaaa aaaagaagaa taaaatcaat ttttgcccag
     8221 ttttaatcaa aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct
     8281 ggtttatatt cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt
     8341 gtatcacgat cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt
     8401 ttgcgccttt tggcaaagaa gtaatttatc accagtctgg catgtcattg atcgttaata
     8461 tcatctggct gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc
     8521 ttctatgtat cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat
     8581 tggcgttgat gccattcggt gcagaaattg tataaaaaga gaataataaa ggttagaaaa
     8641 cgcatagagt caggtgggat acatgtattt cacctgactc tatgtgtttt cctgtataag
     8701 ttctcccatg tgacttttac gtcttttatt tatatgggat tactgtttat ggaaagttga
     8761 aataattttc attaaagaac tctttctata tttttgaaag cgtagtggag cttcgaatat
     8821 ctgggaagga cattttgcag ggattgaaaa aagatattgt cctatcgaaa aattaatgaa
     8881 tttagacaaa ttgccagcaa caggcttcaa acttagttgc tatcctgtca agatcaagaa
     8941 ggcaagtgcg ggttggataa gagcaggggc aatgatcgaa gagaaaaaga aagaataagc
     9001 caaaacatct gtatggaaga a
//
LOCUS       DACDTH010000095         8919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDTH010000095 DACDTH010000000
VERSION     DACDTH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.098"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="IWK54_002587"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="IWK54_002587"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9594664.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1711..3057)
                     /gene="gor"
                     /locus_tag="IWK54_002588"
     CDS             complement(1711..3057)
                     /gene="gor"
                     /locus_tag="IWK54_002588"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9594665.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3191..4432)
                     /locus_tag="IWK54_002589"
     CDS             complement(3191..4432)
                     /locus_tag="IWK54_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9594666.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(4450..5373)
                     /locus_tag="IWK54_002590"
     CDS             complement(4450..5373)
                     /locus_tag="IWK54_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9594667.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5594..8086)
                     /locus_tag="IWK54_002591"
     CDS             complement(5594..8086)
                     /locus_tag="IWK54_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9594668.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8093..8560)
                     /locus_tag="IWK54_002592"
     CDS             complement(8093..8560)
                     /locus_tag="IWK54_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9594669.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8696..8769)
                     /locus_tag="IWK54_002593"
     tRNA            complement(8696..8769)
                     /locus_tag="IWK54_002593"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8733..8735),aa:Arg,seq:acg)
     gene            complement(8827..8900)
                     /locus_tag="IWK54_002594"
     tRNA            complement(8827..8900)
                     /locus_tag="IWK54_002594"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8864..8866),aa:Pro,seq:tgg)
BASE COUNT         2478 a         1884 c         1485 g         3072 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
     1081 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
     1141 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
     1201 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
     1261 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
     1321 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
     1381 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
     1441 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
     1501 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
     1561 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
     1621 aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa
     1681 taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc
     1741 tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc
     1801 cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat
     1861 tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa
     1921 tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt
     1981 ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac
     2041 tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata aacgttctga
     2101 taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc
     2161 aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt
     2221 tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc
     2281 accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata
     2341 ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc
     2401 ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg
     2461 ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc
     2521 agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa
     2581 aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc
     2641 tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg
     2701 actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct
     2761 ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa
     2821 atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc
     2881 caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt
     2941 tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt
     3001 tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt
     3061 gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa
     3121 aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc
     3181 agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg
     3241 gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat
     3301 gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat
     3361 ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag
     3421 ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt
     3481 cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc
     3541 ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt
     3601 ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc
     3661 cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg
     3721 acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt
     3781 ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac
     3841 atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat
     3901 aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa
     3961 tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt
     4021 ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg
     4081 gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg
     4141 cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt
     4201 catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa
     4261 ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat
     4321 tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg
     4381 ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca
     4441 tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg
     4501 gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca
     4561 gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt
     4621 ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc
     4681 attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc
     4741 ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc
     4801 acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt
     4861 ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc
     4921 ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc
     4981 ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat
     5041 atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac
     5101 tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat
     5161 tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc
     5221 taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa
     5281 ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc
     5341 aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat
     5401 agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa
     5461 ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt
     5521 gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac
     5581 acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg
     5641 tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa
     5701 gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg
     5761 caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt
     5821 tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga
     5881 ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca
     5941 agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt
     6001 gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg
     6061 ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag
     6121 aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg
     6181 ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca
     6241 actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac
     6301 tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca
     6361 tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca
     6421 tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac
     6481 gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca
     6541 gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact
     6601 ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt
     6661 ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta
     6721 attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt
     6781 catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga
     6841 ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga
     6901 tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat
     6961 ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg
     7021 gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt
     7081 tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg
     7141 gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat
     7201 gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg atgacctttt
     7261 tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa
     7321 gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag
     7381 ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt acagggttat
     7441 tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa
     7501 ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg
     7561 taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca
     7621 ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt
     7681 ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta
     7741 ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc
     7801 tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag
     7861 ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt
     7921 cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg
     7981 ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg
     8041 caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc
     8101 catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt
     8161 catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt
     8221 tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac
     8281 tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc
     8341 ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact agataaccac
     8401 gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga
     8461 ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta
     8521 tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact
     8581 aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta
     8641 aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt atcgacgcgc
     8701 ccaagaggag tcgaacccct aaccttttga tccgtagtca aacactctat ccagttgagc
     8761 tatgggcgca tattcaattt aaaaaaatta taaatcattt gaaaacaaaa agcaagataa
     8821 aaaaaatcgg gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta
     8881 ccaagctgag ctacttcccg tttaaaaaat aaaaaagac
//
LOCUS       DACDTH010000096         8904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDTH010000096 DACDTH010000000
VERSION     DACDTH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.099"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(305..661)
                     /locus_tag="IWK54_002595"
     CDS             complement(305..661)
                     /locus_tag="IWK54_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9594670.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /locus_tag="IWK54_002596"
     CDS             complement(675..1085)
                     /locus_tag="IWK54_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9594671.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1432..3618)
                     /locus_tag="IWK54_002597"
     CDS             complement(1432..3618)
                     /locus_tag="IWK54_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9594672.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3836..4519
                     /locus_tag="IWK54_002598"
     CDS             3836..4519
                     /locus_tag="IWK54_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9594673.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4600..5319)
                     /locus_tag="IWK54_002599"
     CDS             complement(4600..5319)
                     /locus_tag="IWK54_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9594674.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5631..5837)
                     /locus_tag="IWK54_002600"
     CDS             complement(5631..5837)
                     /locus_tag="IWK54_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594675.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6082..6684
                     /locus_tag="IWK54_002601"
     CDS             6082..6684
                     /locus_tag="IWK54_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9594676.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            6825..8105
                     /locus_tag="IWK54_002602"
     CDS             6825..8105
                     /locus_tag="IWK54_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9594677.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8118..8846
                     /locus_tag="IWK54_002603"
     CDS             8118..8846
                     /locus_tag="IWK54_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9594678.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT         2587 a         1667 c         1463 g         3187 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acgtttgaat
     1201 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1261 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1321 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1381 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1441 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1501 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1561 tagtcttcgt tcttcttcta tcgtcgtcac tggttctgct tttatttcat taaagtcgat
     1621 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1681 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     1741 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     1801 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     1861 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     1921 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     1981 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggctat
     2041 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2101 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2161 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2221 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2281 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2341 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2401 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2461 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2521 ataattagtc aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2581 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2641 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     2701 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     2761 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     2821 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     2881 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     2941 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3001 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3061 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3121 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3181 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3241 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3301 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3361 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3421 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3481 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3541 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3601 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3661 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     3721 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     3781 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     3841 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     3901 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     3961 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4021 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4081 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4141 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4201 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4261 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4321 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4381 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4441 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4501 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4561 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttatttg taaagattcc
     4621 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4681 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     4741 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     4801 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     4861 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     4921 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     4981 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5041 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5101 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5161 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5221 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5281 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5341 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5401 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5461 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5521 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5581 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5641 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     5701 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     5761 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     5821 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     5881 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     5941 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6001 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6061 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6121 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgccttagaa
     6181 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6241 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6301 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6361 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6421 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6481 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6541 tctctcatta ctttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6601 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6661 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     6721 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     6781 taattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     6841 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     6901 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     6961 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7021 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7081 ttttaggaga tcacttaaca agcatcgacg atttttttgt tttgaaatct tcggttattg
     7141 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7201 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7261 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7321 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7381 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7441 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7501 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7561 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7621 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7681 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     7741 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     7801 gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     7861 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     7921 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     7981 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8041 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8101 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8161 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8221 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8281 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8341 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8401 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8461 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8521 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8581 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8641 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     8701 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     8761 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     8821 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     8881 agccgatcca aacccttcgg ttct
//
LOCUS       DACDTH010000097         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDTH010000097 DACDTH010000000
VERSION     DACDTH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.100"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IWK54_002604"
     CDS             complement(<1..173)
                     /locus_tag="IWK54_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9594679.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IWK54_002605"
     CDS             complement(593..1714)
                     /locus_tag="IWK54_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP9594680.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IWK54_002606"
     CDS             2042..3451
                     /locus_tag="IWK54_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP9594681.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IWK54_002607"
     CDS             3448..4176
                     /locus_tag="IWK54_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9594682.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IWK54_002608"
     CDS             complement(4304..5215)
                     /locus_tag="IWK54_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9594683.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IWK54_002609"
     CDS             complement(5232..6239)
                     /locus_tag="IWK54_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9594684.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IWK54_002610"
     CDS             complement(6236..8365)
                     /locus_tag="IWK54_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9594685.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IWK54_002611"
     CDS             complement(8370..8477)
                     /locus_tag="IWK54_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594686.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACDTH010000098         7857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDTH010000098 DACDTH010000000
VERSION     DACDTH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.101"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..413
                     /locus_tag="IWK54_002612"
     CDS             <1..413
                     /locus_tag="IWK54_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594687.1"
                     /translation="KKAEKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHA
                     MILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPL
                     EYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(539..1216)
                     /gene="nth"
                     /locus_tag="IWK54_002613"
     CDS             complement(539..1216)
                     /gene="nth"
                     /locus_tag="IWK54_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9594688.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1223..1921)
                     /locus_tag="IWK54_002614"
     CDS             complement(1223..1921)
                     /locus_tag="IWK54_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9594689.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2189..3373
                     /locus_tag="IWK54_002615"
     CDS             2189..3373
                     /locus_tag="IWK54_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9594690.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3379..3822)
                     /locus_tag="IWK54_002616"
     CDS             complement(3379..3822)
                     /locus_tag="IWK54_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9594691.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4048..4335)
                     /locus_tag="IWK54_002617"
     CDS             complement(4048..4335)
                     /locus_tag="IWK54_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594692.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4506..5858)
                     /locus_tag="IWK54_002618"
     CDS             complement(4506..5858)
                     /locus_tag="IWK54_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9594693.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6131..6577)
                     /locus_tag="IWK54_002619"
     CDS             complement(6131..6577)
                     /locus_tag="IWK54_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9594694.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6813..7739)
                     /gene="cysK"
                     /locus_tag="IWK54_002620"
     CDS             complement(6813..7739)
                     /gene="cysK"
                     /locus_tag="IWK54_002620"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9594695.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2132 a         1591 c         1275 g         2859 t
ORIGIN      
        1 ataaaaaagc tgaaaaattt tgtttattat taaataaatt tactttaatt gctcctatcc
       61 ttgcatttat tattttttct gtacttttaa gcactactct taaaggaaaa tttatggaaa
      121 gaagctctca tgcaatgatt ctcacttttt tatggttgtt gttcaccaga atctatattt
      181 ttctgatgag cctgaaaccg cccaaatcca ttagtttatg tttagttata aatggtattt
      241 ttttattatc acttatcatt tttattacac ctttagatag atacgtcact tatttatata
      301 atcctttgga atactggact tattttatag gtatattgga aggcattatt ttctacatcg
      361 ggtattttcc caataaaaat aacaactttt atttttatcg caataaattg taataagtta
      421 ccatcctagc tactgagtat gtacatttac tttttcatgc aaaaaagata aacgaaacag
      481 cttttctcca taataacaag ataattcata catttatttg caattaaaga aaccacgtct
      541 acaggcgtgg tttctttttt agcgcctttt cttttagacg catccgattt ttcccatctt
      601 gacatattga tagcaaagga catacctcgc acttgggatt tcttgccgtg caatgatatc
      661 taccaaaaaa aatcagtgta tgatgtgtct ttacccataa ttcttgtggt actttccgca
      721 tcagtgtttc ttctacttcc atgaccgtag catctagttt gcagatcctc aggcgttttg
      781 agacacgttc cacgtgggta tctactgcga ttgctggtat cccgaatgca tcccccaata
      841 cgacatttgc tgtctttctg cctacaccag gaagagacat caattcttct cttgacgttg
      901 gcacttgccc atcgaaacgt tcaatcagct gctgtgcaca cgctttgatg ttttttgctt
      961 tatttcgata caaaccaatc gtttttattt taagaatgat ttcatcgatt gaagcttctg
     1021 ctaacgcatc gggtgtcgga aaagaggcaa acaaatctgg cgttgcttta ttcactgata
     1081 catctgtcgc ctgggcactt aagatcactg caatcaataa ctcaaatgga ttattgtgct
     1141 tgagttctcc atgtgcttcg ggaaacattc catacatcgt ttctagagct tccattgttt
     1201 tttgtttgct taacatattc cgctactcct ccaaccaatt tttcagtgag actttcggca
     1261 attcttcttg ttgagctgac tgttctactt ctctttgaag aagctgctgt tttcttcgtt
     1321 tttggtcttc ttccacttgt tgttttgtac gtagattttt tctttcccac gatagcaaaa
     1381 tgcggtcaac gtaattaaaa ctataagcct gatttaatac ggcttcccgc agtgctagct
     1441 tcaaaatttc cggctgataa tggtcttctt ctagccattg tccgatccgc tggaactcga
     1501 tcgaagaaag cgggcgtcca aattcttgtt caaataattg atacactgac tggatttctt
     1561 tttcttgtgt tttttcttcg tatttttttt cttgtgtttt taaatactca cctaatagat
     1621 catagatggg gtaaagatta tatcgatctg cttttttacc gttgacaatc gtcgtttcta
     1681 tcttgataaa tccatttgtt acgagacgat ccagtatttg aaaaatctga tcttgtttga
     1741 ttcccatatc catagcaatc atctgcaaat cagggaaagt atctccttca agctgtgcca
     1801 tatgcaactg tagaacaaat aaaaattctt cagattgtag accgatccta tgataatttt
     1861 taagtaaaag attagatatg actgtttgtc cagcgcttaa gtaattttct aaattcaaca
     1921 tgatttctcc tccgctcaat cacagtatac gtaaaaaaaa catgggaagc aagaacaggt
     1981 ttcttttcta tttacttcac tgatcggtca gatttacaat gtcgtctact tgatttttgt
     2041 ttgttcatgt ttttgatgcg tatcttcact tttcggaata aataaattaa gtagatttcg
     2101 ctgttcctta gaatgttgac aaggaaacaa aattactata cactattttt gtttccaagt
     2161 taacattatt gcataaagga gtttttacat gtacagtttc acaaataaac attctaaagt
     2221 ttttggatac gtctctgtct tattcactgg attaggattt actatcgtca gtcctgtact
     2281 tccgttttta acgctgcctt attctcactc catacatcag caagctttct atatcacttt
     2341 gctcatgtct gtttatgctc tagctgcctt cttatctgcc cccattctcg gtagcttaag
     2401 tgatcatttt gggcgtcatc cgatccttat tatcagttta ttaggatcaa gtatcggata
     2461 tttgcttttt ggtttaggaa attcgatatg gatgttgttc cttggtcgta tcattgaagg
     2521 attgacggca ggagaaatct ccaccttata tgcttacttt gccgacatca cggaacctaa
     2581 tgaaagaacc aaagtttttg gatggatggg tgctttagtc ggtataggaa caacgctggg
     2641 tcctattata gggggtctat tagctgagtt ggggaattct gttcctatat ttattggagc
     2701 actctttact ttcttaaatg ccgtttatgg ctacacattt atgctagaaa gccttccaat
     2761 aaaaaaacgt tcagtagatt taagtttctc ccatgtaaga ccttttcacc aattacaaca
     2821 attattcaag atttcatccg ttattccttt attgacagca ggttttgctg tttggttggc
     2881 agctggctct cttcaatcta tcttttctca attttcgatt gacgcctttc agtggaaggc
     2941 tgggttggtt ggtttaagct tctctttgat tggaatcttg gatactgtct cgcaattgtt
     3001 cattatgcct cgattgctca aaaaattttc agaacagcag atcacaagaa tagggatgtt
     3061 cagtgaaatc ctcgcctatc tattcatcac attatctgga attcttctac tgccgatttt
     3121 gtttttattt gggattatct gttatggttt cggtgattct atttttactc ctgtattcaa
     3181 tggccaacta tccaattctg tctcagataa ccagcaaggt cttgtaatgg gcggtaccca
     3241 aagtatacaa tctctatcgc gcgtcatcgg cccgctaatc gcaggacagc tttatgctgc
     3301 agcaccattt cttccgacgg tattcggatt tttgcttatg atttttgcct attatcacta
     3361 caataaaaaa tagtagcttc atttgctact tcgtataaat tctctgtatc gaccgataaa
     3421 acgaaatact gtgtcaaatt cttcttttga catattttca aatacagtag catctcgctg
     3481 tacccatttt tgatggagtt gctcgtgtct attccttatt ttttgtccga ttggcgtcaa
     3541 ttgaaaatag atttcttttt tattctcttc gctttgataa gtgtttatga tccctctagc
     3601 tatcagtttt tttgttagct tactgatggc tcctctagtc atgtaactag cattcgctaa
     3661 ttttgtcacg ttaggatcag ggattttccc gatatgttca atcacttcta gttcatttag
     3721 agtaaatcct gcaaattctt tttccatttc tggtttatta cgatgaataa tctcattagc
     3781 taaatccaga tattcttcta ttagcagttg tcttttatcc atttctacac cttctttttc
     3841 tttcattata ttgttttttt gtttcctatt caacaaagat aaaaatattt tgtaggaaaa
     3901 cccaatctga aaccaagaaa aaaaggggtt gacaaagctt gtcataaccc ctttttctga
     3961 ttaaaagata gtaaagagac agtcatcggt aattgtgatg attttttgtc atcacttaag
     4021 gatgagcggc cttttcaaaa actgtcttta aagatcatca tcagtttcta gcacatatgg
     4081 ctgaccttca tatgtgtact ctgtattcaa tgtgccgtca ttttcatctg agtagatgcg
     4141 tctactgaaa ggaaaacgat gagaaacatt atctttggcg tcatttgctg gcatcgtgta
     4201 atattcctct tgcgtttcat tcaactgaga gagtacttga tctgccaact catcaatatt
     4261 gatcgttaat ttcttcgaag cacttttata atcttctaaa aatgcatctt gttggacaaa
     4321 atattcttta ttcatcttct ttctcacctt tcttttcaaa atcaaataaa gctgcttgtt
     4381 cttttatgta acagctttat ctttgctgct tattgtgaca aaaaattcga agaaagacaa
     4441 ctaattgtac ttttcacaaa aaaagacaga gggtcgtgac aaaattgtgt cacaaccctt
     4501 tttctttatt gtgcagtttc gatttcgcct agcgctgctt gtgcaagtag gttcaagtaa
     4561 ttccatgggc ggtcaaaatg tggttggaag aagaaatctg aaagtgcgag atcttctaca
     4621 gtcattttat tttgaacagc taaagacatg gtatttgctg attgtgtaat gtcgtatttg
     4681 gacatgaact gagcacctac gatacgattc gttccttttt catagactag ctccatcatt
     4741 actttttcag tagttggcat gaactctgga cgataattat cttcaaatac agtcgcttgg
     4801 acttctagat cgttcaaagt tgcgctttct ttggtcacac ctgtcgaacc gatcgtccag
     4861 ccaaataggt aaagtcctga agttccttga gtaccacgat acgccatttt atgatctgtc
     4921 aagttatttc ccacaagcaa accttgacga acggcatttg ttgccaaagg aatatagttt
     4981 tgcgtgccac ttggattgta gttgactact gcagagtctc cagctgcaaa aatgtctgga
     5041 ttacttgtct gcatgtattc attcactttg attgccccgt ttggcagtgt atctactttt
     5101 ccttcgatca gttttgtatt tggacggaag cccacacata aaatgaccat atctgtatca
     5161 aatgtatctg ttggtgtagc tacttgtttc acttttcctt cttcatctgt gataaattca
     5221 gtaacatttt cgcctagcgc aagagtaaca ccgcggtctg tcaattcttt ttctaagata
     5281 tctgtgaatg gtttatctag atatttattc aagatgcgat ctaatccatc aatcaatgtc
     5341 acttgttttc cagattcgac aaaagcttct actaattcaa taccgatata tccaccacca
     5401 acaattacta cacgttttgc ttctttcgct tgtgcaataa tttcatttgc ttgattgtag
     5461 tttttgcata ggagtacatt tttcgcatcg attccactaa tcggtgggat aataggccat
     5521 gaaccagttg tcatcactaa tttatcataa gaaaccgttt ctgtttctcc tgttttcaga
     5581 tcttttgccg ttactgtttt attttctgta tcgacgtctg ttacatcgtg ttccatttta
     5641 actgttgctc ctaatgaaga taattcttca ggatttgagt agaacaagcc tgctgggtcc
     5701 tttactactc cgccgacata aagtgcgata ccgcaagata agaaagaaat attgtcattt
     5761 ctttcatata ctgtcacttc tgcttctgga tgatttttta aaatactttt tacagctgca
     5821 gtacctgcat gagtacagcc aattactact actttcattt atggtccctc cgattaacat
     5881 tttgtccttc gacaaaacta ctataacaga aataaaacgg atacaacagt ttaatgcttg
     5941 tgaattcaca aaggtttgtt tgtagtttca ttccttttca agcataagaa cccaatatct
     6001 acaagattct tattttaata atattcactt ttatatctaa gattgtgaat aaaagacaaa
     6061 aaaataaaaa agctagcgtt ttttttacac agctagcttt ttttaggtat aacacttgta
     6121 gaaactgttt ttacatttgc gccttttttg ctgactcttt acaatccgga cagagtccat
     6181 acaattccaa ccgatgttca ttaatctcaa aatcagtcag tctgcttgca gccatttcta
     6241 tatcttcaag ccctgggtaa tgaaaatcaa caatcttacc gcagttttca caaattacat
     6301 gataatgacg ctttgaactg aaatcaaatc ggctagaagc atcgccgtac ttcatttctt
     6361 gaacaaatcc aatcgaagta aataagcgaa ggttattgta aacagtcgca acactcatat
     6421 ttggaaaccg atgttcaagt gctttataaa tctcatcagc cgttggatgg gaatgatgtt
     6481 caatcaaata ttctaaaacc gcgtaccgtt gaggtgtgat tcgaatgttc tcttttttta
     6541 acttttcgat aacctcttcg actaacttat tttccatgta aacaccccac gttgttattc
     6601 tcgtcttatc atactatttt tacattttaa aatccactta acatgaaaag aaaattcaat
     6661 tttattgtat tttaatgaaa aaagacgtaa aacagtcgcg ttcactattc tacgtctcta
     6721 ttcaaataat ttttctcatc ataaattttt gtgtgttctt ttcatcttta aaagtttttt
     6781 acataaacct atctggagaa aaaccaagtg ccttatatct cttggtaaag ggctgtagat
     6841 aaataacgtt caccattgtc gggaaccaca gctaatacct tcttgccttt tcctaattct
     6901 tttgcgactt ttagcgcggc agcaattgca gcgccagatg agatgcctac taaaatccct
     6961 tcttttcgcc caacttctct agctgtttcc atcgcttcgt ctccactaat agaaaggact
     7021 ttatcataca cagaagtatc cagtgtttta ggaacaaaac ctgtaccgat tccttggatt
     7081 ttgtgaggtc ccgcttcttt cccttctaaa atagcagatt ctgctggttc taccccgatt
     7141 aattcgattt tcggatacac acgttttaat tctcggccag cgccagtgat cgttcctcct
     7201 gtaccgatac ctgcaacaaa cgcgtccaag ccatttactc caaatgcttg atgtatctca
     7261 ggaccagtcg tcttctcatg cacaagagga tttgcttcat tttcaaattg taatggtaaa
     7321 aagtagccgt tttcttttgc caaacgttct gcttctctga ttgatccaga aataccatcc
     7381 gcaccaggag tcaaaatcaa ctctgcgccg taccctttca tcaataaacg tctttcgata
     7441 ctcatcgtct caggcatcac gatgattact ttatatcctt ttgcaactcc taccatcgac
     7501 aatccgattc ctgtatttcc agaagtaggt tcaatgatag tatcacctgg tttcagtaga
     7561 ccatcgtgtt ctgctttttc tatcatactt aaagcaatac gatcttttac acttcctcct
     7621 ggattaaaaa actcaagctt tacaaaaaca tctgctgaat cttctggtac gattttattt
     7681 aatttgacga ttggcgtttt gccaatcaat tcggtgactg agttatagat agctgtcatc
     7741 ttcaattcct ccttgtcttt gctcttctta cttatcataa tacaatctgt tcatttatgg
     7801 cgagtgattc gtctttgact agcaaaaaaa agacaaagat ataccatata tggtaat
//
LOCUS       DACDTH010000099         7600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDTH010000099 DACDTH010000000
VERSION     DACDTH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.102"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IWK54_002621"
     CDS             complement(138..428)
                     /locus_tag="IWK54_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594696.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IWK54_002622"
     CDS             complement(546..806)
                     /locus_tag="IWK54_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594697.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IWK54_002623"
     CDS             complement(943..1356)
                     /locus_tag="IWK54_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9594698.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IWK54_002624"
     CDS             complement(1812..2297)
                     /locus_tag="IWK54_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594699.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IWK54_002625"
     CDS             complement(2436..2651)
                     /locus_tag="IWK54_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9594700.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IWK54_002626"
     CDS             2875..3675
                     /locus_tag="IWK54_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9594701.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IWK54_002627"
     CDS             3694..5562
                     /locus_tag="IWK54_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9594702.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IWK54_002628"
     CDS             5555..6046
                     /locus_tag="IWK54_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9594703.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IWK54_002629"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IWK54_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9594704.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="IWK54_002630"
     CDS             6606..>7600
                     /locus_tag="IWK54_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9594705.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACDTH010000100         7428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDTH010000100 DACDTH010000000
VERSION     DACDTH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.103"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..368
                     /locus_tag="IWK54_002631"
                     /pseudo
     CDS             1..368
                     /locus_tag="IWK54_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            548..1489
                     /locus_tag="IWK54_002632"
     CDS             548..1489
                     /locus_tag="IWK54_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9594706.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1558..2289
                     /locus_tag="IWK54_002633"
     CDS             1558..2289
                     /locus_tag="IWK54_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9594707.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2433..4613
                     /locus_tag="IWK54_002634"
     CDS             2433..4613
                     /locus_tag="IWK54_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9594708.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4633..5115
                     /locus_tag="IWK54_002635"
     CDS             4633..5115
                     /locus_tag="IWK54_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9594709.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5131..5892
                     /locus_tag="IWK54_002636"
     CDS             5131..5892
                     /locus_tag="IWK54_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9594710.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6023..7357
                     /locus_tag="IWK54_002637"
     CDS             6023..7357
                     /locus_tag="IWK54_002637"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9594711.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT         2494 a         1297 c         1637 g         2000 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tcggaaaaag atcgtcaaga aatggatggg acgcttataa
      121 ttgaagtagg agttgaggac agtcaaacct catttctgtt atatgatcga gcatttgttg
      181 agtcccattc acctggcaca gctactagtg ccccatcttt gatgttttac agtaaagata
      241 tctttggact atataaaaaa atgcagacgc aatcagtcac attaggtgat atggtacagc
      301 ttggagaaga atatgtcttt aactttgctg atcccgatgg caactacttt gcggttacag
      361 gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa caggaaagcg aagtcttttt
      421 aattcaaata ataatattat gtaaacaaaa aactttccat gcgtaattat acacaagcaa
      481 ataaatcgtc aaatgcgaac gccttcttta tactgtttag gaacgaaaag aaggaaggaa
      541 gaaatacatg gattatcaag tactgcttta ttacaattat acaaaaatcg aagaaccaga
      601 ggaatttgcc aaaaatcatt tagccttttg caaatctttg gatcttaaag gtcgtattct
      661 agtagctgaa gaaggaataa atggtactgt gtctggaacc gttgaaaaca cgactgccta
      721 tatgaaagca atgaaagctg atcctcgatt tgctgatacg atgttcaaga tcgatccaag
      781 cgaaggacat gcctttaaga agatgtttgt aagataccgt ccagagcttg taagtttaaa
      841 gctagaagag gacattgatc caaaccaagt gacaggtgcc tatctttcac cgcaagaatt
      901 taaagaagct attttagatg aacagacagt ggtgattgat gcacggaatg attatgaata
      961 tgacttggga cattttcgag gggctgtacg cccagatata cgcagttttc gagagcttcc
     1021 agactggatc cgcgagcaca aagaagaatt tatggaaaaa cgggtagtga cctactgtac
     1081 tggtggcatt cgttgcgaga agttttctgg ttggcttgtc cgtgaaggat tcaaagatgt
     1141 cggacagctg catggcggta ttgctactta tggtaaggat cctgaagtcc aaggagaatt
     1201 atgggacggc aagatgtatg tgtttgatga acggatcgct gtcgatatca accaagtaga
     1261 taaacaaatc gtcggtcgcg actggtttga cggaacacca tgtgaacgat atatcaattg
     1321 tagcaatcct gagtgcaatc gtcagattct tgcttccatt gaaaatgaag aaaaacattt
     1381 aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat cgctatgttc aagaccatgg
     1441 aatctctcca gaagaagcag aactgcggtt tacagcttta attaaataga aaacaatagt
     1501 tttgattgtc gaccatttcc aatatgcgct atactagcgt tgaggatggt gatccacatg
     1561 aaatcattga tttatgctat tggggatgtc catggcaaac atgagctttt tgaaaaactt
     1621 attcactatt atgatccaaa gatccatcag cttgttttat taggggatct caatgatcga
     1681 ggtcctcgta caaaagaatg ttttttatta ggaaagcaat tagtcgaaga aacaggagcc
     1741 atttacctcc ggggaaacca tgaagaatat tttttgcaat ttctgcaatc accggaggac
     1801 tggtatgcgc cttatatgag aaatggtgga aaagaaacgt ttgaaagcct gattcaccca
     1861 ggggcatcgg aggaatactc cccaactgaa atctcgatga tgatccaatc gagatattca
     1921 gaactgatta aatttttagt ccagcgcccc ttgtattatg aatgggaaaa ttatcttttt
     1981 gtccatgccg gtgtggattt gacaaagaaa gactggcgta agacaagtct aagagacttt
     2041 atttggataa gggaaccatt tcatgaaggg aaaaacaata caggtaagac aattgtattt
     2101 ggacatacga ttacaccaat gctccatgga gatatgcaga caacagacct ctggattcag
     2161 gatcataaaa tcggaatgga cggtggggct gtattcggag ggtcattgca tggggttgtt
     2221 tttgacaagg aaggtattct tcaagatatt gaatatcaga acaacgaagg accttggcat
     2281 ggaatctgac taatggaaag aatgaaggag ctgtgacttt gttgcggctc tttttatttt
     2341 tatagagaat attgtttctt tcagtagttt aacccaggat aaaaagtatg atagaataaa
     2401 gaagcaattt ttagcagaaa agaggcattt taatgacgga aaatttgaac cagacgatca
     2461 ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa gcagttgact actgtactta
     2521 atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg ttaccgaaaa gaaatgactg
     2581 gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg gtatgcctat cttgaaaact
     2641 tagaaaaacg taaaactgaa gtgattcggc taatcgatga acaagggaaa ctgactcccg
     2701 agttagaagc agaaatcacc caagccgtga aaatgcaaca agtggaagat ttgtatcgtc
     2761 catacaaaca aaaacgacgg acaaaagcaa cgattgctaa agaaaaagga cttgaaccac
     2821 tcgcgaagtg gctgatgcag cttacagatg gagaggtgca atctgaagca gaaaaatata
     2881 ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt gcacggtgct catgagatta
     2941 ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg gattcggtct tatacgtata
     3001 acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc agatgaaaaa ggcgtatatg
     3061 aaatgtatta tgactttgca gaacccgtcc ataaaatggt ttctcatcga atattagcta
     3121 ctaatcgtgg agaaaaagaa gatattctga aagtattctt tcaagtagat gaagcagcaa
     3181 ttttagctta cttggatcgc caaatagtca aaaatcctgc ctctccttct tcttcattcg
     3241 tcagagaagc ctatcaggat agttataaac gctttattca gccagcaatc gagcgagaac
     3301 tgcgcaatga attgactgaa aaggcagatg agcaagcaat cgctattttc ggcgaaaacc
     3361 tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt tgtcctggga tttgatccgg
     3421 cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac agggaaagtt ttagcaatcg
     3481 aagttattta tcctcataaa cctgcagcgc aagcaaaaag agaagctgct ggaccagctt
     3541 ttatccaatt aattaataaa tatcaagtag atatggtagc tatcggaaat ggtacagcca
     3601 gtcgggaatc tgaacttttt gtggctgagc agttgaaatc cgccgaccat aaaacctatt
     3661 acgctatcgt caacgaagca ggcgcttctg tttactccgc tagcgaaatt gcacgaaaag
     3721 aatttccgca tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt cgcctgcaag
     3781 atcctttagc tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc cagtaccaac
     3841 atgacgtttc tcaaaaacgt ttggctgaac aattggattt tgttgtggaa acagcggtta
     3901 accaagtagg tgtggatgtg aatacagcaa gcccgcagct cttgcagcat atatcaggat
     3961 tgaataaaac aactgcccaa aacattgtca tttatcgtga agaaaacgga gaattcaccg
     4021 cacgaactca attgaaaaaa gtgccacgct taggaccaaa agcatatgaa caagcaatcg
     4081 gattcttgcg agtccctggt gggaaaaaca ttttggacaa cacaggtatc catcccgaaa
     4141 gttattctat tgcaaaagaa atattaactt cagtccaact ttcagaaaaa gagcttggta
     4201 cagaagaagc agtagaaaaa ttgacacgat tatccgtaga gaaactggct gaaagcctat
     4261 cagtcggaga agaaacgtta gccgatattt tagctgggct gacacaacct ggacgagata
     4321 tgcgtgatga gatgccagcg cccttattac gtacagatgt attgagtatg gaagacttga
     4381 aaccagggat ggaattgact ggaacggtcc gtaatgtcat tgacttcggc gcatttgttg
     4441 atatcggtgt gaagcaagac ggacttgtcc atatatcgaa actcagcaaa aaattcgtca
     4501 agcatcctac agatgtcgtg tcagtaggag atatcgtaac agtttggata gaacaagtcg
     4561 atacgaaaaa aggcagaatc agtttgacga tgctttcccc ttatgaagag taacaatgag
     4621 gagtaacaaa aattgacaca agaagaactg cagcagttag tagaagagat atcattaaaa
     4681 agcttccagc ttcctttcaa gcaccaagca atgttcaatc cacgcttacg tacaactggc
     4741 gggcgctatc atttaaacga tcatcattta gattttaatg cgaagctatt tgaagagtca
     4801 gacgaagaga cgattgcagg aatcatcaaa catgaacttt gccattatca tcttcatcta
     4861 gctggaagag ggtatcagca taaagatatg gatttcaagt atcttttgaa aaaaacagga
     4921 gggttgcgct atgcaccagc cttgacgaag aaacagataa agatggagta ttatcgatgc
     4981 caagattgtt ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa atatcgttgt
     5041 ggaagatgtc atggagaaat caaatggatt gagacaaaaa gagaagactt taatgaagca
     5101 caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag gaatctaaaa
     5161 gactggaatc agctattaaa ttttttagcc gcacctgaag agggaaattt tgaggaactg
     5221 acggcaccta cacttatttt agctggaaac tgtcttccta tcttagctga taaagcagca
     5281 caaatgtatt taaatggcca agtagatcaa ttgtttttgg tcggaggggt ggggcatgcg
     5341 actagaatcc tttatgagaa ttttgaaaag caaggatttc attttgaaga tggcatgagt
     5401 gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg atttgcccga taaggctttt
     5461 ctgatagaat cgaagtctac aaattctgga gagaacgcca ctttttcact ggagattctt
     5521 cattccttag gagctgtacc agaaaaaatc ttattgatga atgatccgac actgcaacgc
     5581 aggactcgag caacttttga gaaagtatgg caaaatgaac aaactatttt cgcgaatgct
     5641 gtgccatttg tgccggaaat ccttcacttt tctgaagaga tcatctttac agcaaaagaa
     5701 ctgaatcatc aatggcctaa ggaatatttc catgcgctag tattaggaga aatggaacgt
     5761 ctccatgacg atgagaacgg ctatggtccg aaaggaaaag attttattcc tcacattgat
     5821 atatcagagg aagtttggtc ttcttacacc agaattaaag agtcaataaa aacggatttt
     5881 tcccgtactt gaccatttta gcaagcgaat cctcctttcc aaataaattt ggttgaaatt
     5941 ttcaatcgtt tataagataa tgaggagcgt gttgaatgta atagctaaaa gaatggacgc
     6001 tagataagag aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt ttgttggtat
     6061 caaaggttca ggaatgagct ccttggctct tgttttacat gaacaaggtc taaatgtcca
     6121 aggatcagac attgaaaaat atttctttac acaaagagat ttagaaaaag caaatatcac
     6181 gattctgcca tttaacgcag ataatgtaaa accagggatg acgatcattg caggaaatgc
     6241 atttcctgat tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg aagtgattcg
     6301 ctatcacgac tttattggtc attttatcca aaattatacg agtatcgctg taacaggctc
     6361 tcacggaaaa acaagtacga ctggactgct ctctcatgta ttgagtggtg tccgtccaac
     6421 aagttatcta attggagatg gaacaggaca tggtgatccg caagcggaat tcttttcatt
     6481 tgaggcctgt gaatatcgcc gtcatttcct tgcctattca ccagactatg caatcatgac
     6541 caatatcgat tttgatcatc cggattatta tacaagtatc gacgatgtat ttacagcttt
     6601 ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg tatggtgacg atgcttattt
     6661 acgcaaatta aaagcaaatg tgccgattta ttattatggt gtcacagaaa acgatgatat
     6721 ccaagcacga aacatcgaac gcacaacttc tggttctgct tttgatgtct atcatgggga
     6781 tgaatttgtt gggcacttca ctgtaccagc attcggtaaa cacaatatct taaatgcttt
     6841 aggtgttatt gcagtagctt atttcgaaaa actggatctg aaagaagttg cagaagaaat
     6901 gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag acatgaccgt
     6961 agtcgatgat tatgcgcatc accctgcaga gatcaaagca acgattgatg gcgcacgcca
     7021 aaaatatcca gataaagaaa taattgcagt tttccaaccc catacgttta cgcgtacgat
     7081 tgcattgatg gatgaatttg cagaagcact tgatttagct gataaagttt acttatgcga
     7141 tatttttggt tcggctcggg aagaacaagg aaacgttaag attgaagacc ttggagcgaa
     7201 aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta tctccattgt tagattatca
     7261 tgatgcagta gtaatcttca tgggtgctgg agatgttcaa aaatttgaac aagcgtatga
     7321 aaaactttta agcagcacga cgaaaaatgt actgtaatac aattactgca gtgtccttat
     7381 aaaaatagaa agagggggag tggcaaaaat cttgccaagc cccctttt
//
LOCUS       DACDTH010000101         6993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDTH010000101 DACDTH010000000
VERSION     DACDTH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.104"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(134..592)
                     /locus_tag="IWK54_002638"
     CDS             complement(134..592)
                     /locus_tag="IWK54_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9594712.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(605..1930)
                     /locus_tag="IWK54_002639"
     CDS             complement(605..1930)
                     /locus_tag="IWK54_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9594713.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1947..3359)
                     /locus_tag="IWK54_002640"
     CDS             complement(1947..3359)
                     /locus_tag="IWK54_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9594714.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3376..4140)
                     /locus_tag="IWK54_002641"
     CDS             complement(3376..4140)
                     /locus_tag="IWK54_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9594715.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4244..5119)
                     /locus_tag="IWK54_002642"
     CDS             complement(4244..5119)
                     /locus_tag="IWK54_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9594716.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5374..5458)
                     /locus_tag="IWK54_002643"
     tRNA            complement(5374..5458)
                     /locus_tag="IWK54_002643"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5422..5424),aa:Leu,seq:caa)
     gene            complement(5476..5547)
                     /locus_tag="IWK54_002644"
     tRNA            complement(5476..5547)
                     /locus_tag="IWK54_002644"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5513..5515),aa:Gln,seq:ttg)
     gene            complement(5557..5629)
                     /locus_tag="IWK54_002645"
     tRNA            complement(5557..5629)
                     /locus_tag="IWK54_002645"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5594..5596),aa:His,seq:gtg)
     gene            complement(5691..5775)
                     /locus_tag="IWK54_002646"
     tRNA            complement(5691..5775)
                     /locus_tag="IWK54_002646"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5739..5741),aa:Leu,seq:caa)
     gene            complement(5796..5866)
                     /locus_tag="IWK54_002647"
     tRNA            complement(5796..5866)
                     /locus_tag="IWK54_002647"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5832..5834),aa:Cys,seq:gca)
     gene            complement(5912..5983)
                     /locus_tag="IWK54_002648"
     tRNA            complement(5912..5983)
                     /locus_tag="IWK54_002648"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5949..5951),aa:Gln,seq:ttg)
     gene            complement(5995..6067)
                     /locus_tag="IWK54_002649"
     tRNA            complement(5995..6067)
                     /locus_tag="IWK54_002649"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6032..6034),aa:His,seq:gtg)
     gene            complement(6070..6143)
                     /locus_tag="IWK54_002650"
     tRNA            complement(6070..6143)
                     /locus_tag="IWK54_002650"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6109..6111),aa:Trp,seq:cca)
     gene            complement(6150..6230)
                     /locus_tag="IWK54_002651"
     tRNA            complement(6150..6230)
                     /locus_tag="IWK54_002651"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6194..6196),aa:Tyr,seq:gta)
     gene            complement(6232..6307)
                     /locus_tag="IWK54_002652"
     tRNA            complement(6232..6307)
                     /locus_tag="IWK54_002652"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6272..6274),aa:Phe,seq:gaa)
     gene            complement(6343..6415)
                     /locus_tag="IWK54_002653"
     tRNA            complement(6343..6415)
                     /locus_tag="IWK54_002653"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6380..6382),aa:Asp,seq:gtc)
     gene            complement(6425..6497)
                     /locus_tag="IWK54_002654"
     tRNA            complement(6425..6497)
                     /locus_tag="IWK54_002654"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6462..6464),aa:Val,seq:tac)
     gene            complement(6513..6584)
                     /locus_tag="IWK54_002655"
     tRNA            complement(6513..6584)
                     /locus_tag="IWK54_002655"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6549..6551),aa:Glu,seq:ttc)
     gene            complement(6599..6688)
                     /locus_tag="IWK54_002656"
     tRNA            complement(6599..6688)
                     /locus_tag="IWK54_002656"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6652..6654),aa:Ser,seq:gga)
     gene            complement(6728..6801)
                     /locus_tag="IWK54_002657"
     tRNA            complement(6728..6801)
                     /locus_tag="IWK54_002657"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6765..6767),aa:Asn,seq:gtt)
     gene            complement(6811..6926)
                     /gene="rrf"
                     /locus_tag="IWK54_002658"
     rRNA            complement(6811..6926)
                     /gene="rrf"
                     /locus_tag="IWK54_002658"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2106 a         1547 c         1146 g         2194 t
ORIGIN      
        1 taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat ctagtgattg
       61 aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc tatttaactt
      121 ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct tggttttgtt
      181 ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta tctttttgtg
      241 cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt acctcctggt
      301 tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct aaacccatat
      361 ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg gtaggaaata
      421 tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc gttatagcgt
      481 atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt aatggaataa
      541 tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc actttttatc
      601 acctctactt taatgctggc caatattttt tgtttacttt gatcatttca tctagtattt
      661 tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt aaagcttttt
      721 gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact tgtgcctcca
      781 tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc acaccagttg
      841 caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga atcgcccctt
      901 gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa atagctatat
      961 cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc acaataccgc
     1021 ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg accatattag
     1081 cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag taatactcta
     1141 agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc atttgaaagg
     1201 ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct tttgttatta
     1261 gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa atcgatgtat
     1321 accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga tcataaccgt
     1381 aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta atcattttta
     1441 cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt gtataattca
     1501 aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat aaacctttga
     1561 ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct actaatccat
     1621 gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca cgaatttgtg
     1681 agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca cgaatgcgaa
     1741 tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata tccatatttc
     1801 tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt tcattgttca
     1861 aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata gtgataatct
     1921 gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa ttctgccata
     1981 acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg tttaatgatc
     2041 ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc cactacctct
     2101 acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc tccaaacgct
     2161 tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa ctctgtttct
     2221 tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt ctgaatgttt
     2281 ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa aattccgatt
     2341 ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc aacaatacat
     2401 gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc tgctaaaccg
     2461 gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact aaattcaaca
     2521 ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat tcctttgacc
     2581 atctctttat tttctttata agctgcttta tagatagcta gacatatagc tggtatacca
     2641 aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc tccgttgata
     2701 aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct tcctaaatcg
     2761 taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa acgattcaaa
     2821 aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc aaaattatca
     2881 atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat tgtttgtaga
     2941 atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac tactggtaat
     3001 ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat aattccacca
     3061 aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa ggtagcaatc
     3121 gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa accgattgca
     3181 actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc tgcaccaatt
     3241 actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc taatggcatc
     3301 gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt aacattcatt
     3361 tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag gatcattttt
     3421 cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac gaggatacac
     3481 aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca tgacttcgat
     3541 cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca cactcgtatt
     3601 gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct caaaataatc
     3661 gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat catatttttt
     3721 tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat gatgactgtc
     3781 taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat tccattcctg
     3841 atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg atctgaactc
     3901 tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg tcaatgttaa
     3961 atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat gctcgaaacc
     4021 cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag catccataat
     4081 cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca cttttcccat
     4141 ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata gtccatttac
     4201 tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag attcttgcta
     4261 gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat tgctgttcga
     4321 tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat gtttcagtaa
     4381 aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta accaggaatt
     4441 tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg tttgaatagg
     4501 atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag ctctttaaac
     4561 tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct acttcatttg
     4621 accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact gctatatcat
     4681 attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca tctctatctt
     4741 ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag atatttgaat
     4801 ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca aaatccataa
     4861 attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct ttagaatcta
     4921 atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta gcagaaaagc
     4981 caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat acagcattat
     5041 gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat aaaacaaaac
     5101 aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag acaaaaaacg
     5161 acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc ctacataatt
     5221 tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac tcgttttatg
     5281 ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa aatatagcaa
     5341 ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc gaaccggcac
     5401 gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc acgccggcaa
     5461 atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc
     5521 cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga atcgaaccca
     5581 cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc atcataaaag
     5641 atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa taccggcggc
     5701 cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg cgtctgccaa
     5761 ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat ttgaaccggt
     5821 gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta tactattatc
     5881 aatctataaa ataaagaata aattatagaa actggggtag ctggattcga accaacgcat
     5941 gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa aaagggcgaa
     6001 tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca
     6061 cattcgccat ggcaggggca gcaggaattg aacccacact aacggttttg gagaccgctg
     6121 ttctaccttt aaactatgcc cctataaaat ggaggagagt ggattcgaac cactgaaccc
     6181 taaggaacgg atttacagtc cgtcgcgttt agccacttcg ctactcctcc atggtggcgc
     6241 gggacagaat cgaactgccg acacacggag cttcaatccg ttgctctacc aactgagcta
     6301 ccgcgccata ttttaaaatt attaattgaa taacaagaac gacggttccg acgggaatcg
     6361 aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg gaaccagttc
     6421 attatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag atgctctccc
     6481 agctgagcta aacctccaac attcaattaa aatgacccgt acgggattcg aacccgtgtt
     6541 accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa atttgctacg
     6601 gagagtaagg gattcgaacc cttgagacag tttggcacca tctacatgat ttccaatcat
     6661 gctccttcgg cctctcggac aactctccag agtgaaagaa gctcaatact ctttctcaaa
     6721 atgagaactc cggcagtagg actcgaacct acgacatcat gattaacagt catgcgctac
     6781 taccaactga gctatgccgg aataacatca gcgtggcgac gtcctactct cacaaggggc
     6841 aacccctcac tacaatcggc gctaagaagc ttaacttctg tgttcggcat ggttacaggt
     6901 gtatccttct cgctatcgcc accacactgt ggtgttatct tttattgagt aaattttgtt
     6961 cactcaaaac tggatttgaa gtcacataag ttt
//
LOCUS       DACDTH010000102         6706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDTH010000102 DACDTH010000000
VERSION     DACDTH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.105"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(7..1164)
                     /locus_tag="IWK54_002659"
     CDS             complement(7..1164)
                     /locus_tag="IWK54_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594717.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="IWK54_002660"
     CDS             complement(1167..1523)
                     /locus_tag="IWK54_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594718.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="IWK54_002661"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="IWK54_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9594719.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="IWK54_002662"
     CDS             complement(2991..3830)
                     /locus_tag="IWK54_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9594720.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="IWK54_002663"
     CDS             4015..4902
                     /locus_tag="IWK54_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9594721.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5486..6181)
                     /locus_tag="IWK54_002664"
     CDS             complement(5486..6181)
                     /locus_tag="IWK54_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9594722.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6165..6563)
                     /locus_tag="IWK54_002665"
     CDS             complement(6165..6563)
                     /locus_tag="IWK54_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9594723.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2108 a         1319 c          987 g         2292 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
     4981 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
     5041 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
     5101 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
     5161 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
     5221 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
     5281 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
     5341 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
     5401 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     5461 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     5521 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     5581 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
     5641 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
     5701 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
     5761 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
     5821 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
     5881 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
     5941 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
     6001 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
     6061 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
     6121 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
     6181 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
     6241 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
     6301 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
     6361 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
     6421 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
     6481 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
     6541 acaaccgttt caaagatcgt catctctgtt cctcctcgta aaatatcatt tgtctaatag
     6601 tttttattca aaatgtttct agattataaa atttattgaa taacacattc ctaacggaat
     6661 ttgtttcgat attgtgtgat ttctcagagt acaatggatg tatgga
//
LOCUS       DACDTH010000103         6689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDTH010000103 DACDTH010000000
VERSION     DACDTH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.106"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..115)
                     /locus_tag="IWK54_002666"
     CDS             complement(<1..115)
                     /locus_tag="IWK54_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9594724.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            246..698
                     /locus_tag="IWK54_002667"
     CDS             246..698
                     /locus_tag="IWK54_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594725.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            712..1401
                     /locus_tag="IWK54_002668"
     CDS             712..1401
                     /locus_tag="IWK54_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594726.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1526..3877
                     /locus_tag="IWK54_002669"
     CDS             1526..3877
                     /locus_tag="IWK54_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP9594727.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3957..4352
                     /locus_tag="IWK54_002670"
     CDS             3957..4352
                     /locus_tag="IWK54_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594728.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4677..5294
                     /locus_tag="IWK54_002671"
     CDS             4677..5294
                     /locus_tag="IWK54_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9594729.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5335..5634
                     /locus_tag="IWK54_002672"
     CDS             5335..5634
                     /locus_tag="IWK54_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594730.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5668..5835
                     /locus_tag="IWK54_002673"
     CDS             5668..5835
                     /locus_tag="IWK54_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9594731.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5880..6485)
                     /locus_tag="IWK54_002674"
     CDS             complement(5880..6485)
                     /locus_tag="IWK54_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9594732.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2305 a          935 c         1302 g         2147 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
      241 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      301 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      361 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      421 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      481 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      541 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      601 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      661 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      721 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      781 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      841 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      901 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      961 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
     1021 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
     1081 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
     1141 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
     1201 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
     1261 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1321 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1381 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1441 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1501 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1561 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1621 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1681 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1741 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1801 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1861 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1921 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1981 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     2041 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     2101 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     2161 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     2221 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     2281 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2341 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2401 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2461 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2521 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2581 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2641 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2701 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2761 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2821 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2881 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2941 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     3001 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     3061 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     3121 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     3181 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     3241 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3301 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3361 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3421 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3481 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3541 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3601 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3661 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3721 tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt
     3781 tacttatctt gattttattg gagatgaggg gaaagttgca aatggatttt ctgaaaaaat
     3841 tattcaattt caagaaaata gaagtaaact taattaagaa tcgtttacta tttaaacagt
     3901 gtccttttcc tttttaaata tatacaataa aataagtata tgaattgtaa aaggacatga
     3961 tgattatgga agaaatagaa tggaataatc tgactcaaaa taataaaaat aattctggat
     4021 gtggaattat ctttatgttt gttatagttt tgggaatagt agtactttta ataactgtca
     4081 agatagtaga ttactttgcg aatactcctg aagaacaaat acagagaaaa cttcctgagt
     4141 ttgaaagagt accaccttat gatccaagag ataattcatt tcaagtaaaa aaattaaaag
     4201 agtctccaaa tgaaatgtgg tttgaagtta cagttgaatc aagcgacttc aaagaaaaaa
     4261 agaaagaata caattatatt gtaaaggcaa aagtatcaaa aagatttatt tttaacccta
     4321 aattagaaat atatagtgtt gtaatagaat aatagtgtta aaaaaattgt aaaagttaaa
     4381 gaagtcaaat tctgatgtga aaaataaaat ataccatgga agacataaaa gaaaaactgg
     4441 ataactagtt tatttaatgg cagaccgtta aggcctgcca tttttatgta ttttagcgga
     4501 ataaatataa tcatgcggga gtgtaaactt actgtttcaa aaagtatact ttttgacatg
     4561 ccttgatttt ctttgtttat gtatggcata ataaccacat aatacaattt gaaacgtttc
     4621 tgatttaatc tgattgtttt gaaacaattt tagtgaaaaa agggtagata aaaacgatga
     4681 gaaaattttt ttatatccgg gtcagtagtt atgatcaatc gatcgaacga caactgattt
     4741 ccgcgaagga attaggaatc cctgaagaat atatatttgt agaaaaagcc agcggcaaag
     4801 attttaaacg ccctgaatat caattaatga aacggatgtt tcgaaaagga gatttactct
     4861 atctacaatc attagataga ctaggcagaa ataaacaaat gattttagaa gaatggcgag
     4921 aattaactaa ggaaaagcag attgatattt ctgtgttaga catgcctctg ttagatactg
     4981 ctagttacaa gcaactagat gggttagaga cgcttatctc tgacttaatt ctgcaattac
     5041 tcagctatat ggccgaagac gagcggaaac gaattaatag ccggcaaaga gaaggcgtag
     5101 aagctgcgaa acaaaagggt gtaaaattcg gcagaaagaa aattgcttta cctcctgaat
     5161 ttcctgcgat ctattctgac tggaaagcag gaaagataac tgcagtctat gctatggatc
     5221 aattgggcat gaaacgaaat accttttatc gacgggtgaa agaatatgaa cataccttgc
     5281 accacttttc ttgatagata taacagctat tagacgggaa gggagagttt gaaaatggac
     5341 ttaacgaatc aaagggtcgt ttttactgga acgctgcagc aaatgacacg aacccaagcc
     5401 atcggtttgg ttcactcttt aaacggccat tgtcaatcaa gtgttacttc aaaaacaaat
     5461 ttcctggttt gtggtcagta ttctaagtcg ttgtttgatg attctctcta tacaaaaaag
     5521 gaacaaaccg cgcgggactt gattgcgtta ggtcatgaga ttaccatttt atcagaagtc
     5581 gaattttatg cgttaattag tcaacagttg aaagaatggc aacggtattt ataatcgtta
     5641 gtaaaattta caaggaggaa aatttttatg aagcgaaaat cttatgatcg ttggggcatt
     5701 gaaataacta gttttaatcg aaatattgct tttcacgtgg agtgtgactg tggggaatta
     5761 gcaaaaagtg tttatcaaaa aaattacttt tactgtccta aatgccatcg ccgatacggg
     5821 gtacatggaa catagaaacg aaatagtgcg gtagaaaagc gaatgaatgt agtatatttc
     5881 tacaaatcat tcgctttctt tattttataa atagttggtc gagagatccc gttttctttt
     5941 gcaatctctg caactggaat gccttgcttt aacatgtcca ccacggtttt aaaaactaag
     6001 cgtttttgcg gatctgttga gttaatagaa tactcgattg gtcgtccctt atatcggccg
     6061 gctttttgag ccaataatac tccttgccgc tgacgttcac gaattttctc acgctcgttt
     6121 tgtgcaatat aacttagcag tgataaaaac atatcgaaca tagcggtatc caggagttca
     6181 tttccagtat taaaatctaa aaactgtgca tctaatattt ttaacgctac ttttttctgt
     6241 ttcataaaag caactgtatt ttttatatct tcataatctc ttcctaaacg atctagtgat
     6301 gtaacgatga cgcaatcacc ttcacggata taatttagta atttttgaaa ctcaggacgc
     6361 tcagtatctt taccactggc tttttcttgg aataatttat caatgttaag tccttccatc
     6421 attgccaatt gtcgatccaa attttgatct tgtgaagata ctcgaatata gccaattttt
     6481 cccataaaaa actccttttt aaaagtgata taatttacat gtaaaattta cttgtagttt
     6541 tactgctatc atagcatttt taaaaataaa ttttatgtag acttaaaatt tacttcatgg
     6601 agggtttaaa tggatgaaat caaaagtaac aaaaaagcat ttggcattta tttaggagaa
     6661 cctagtacag aacaattaaa ccaattttt
//
LOCUS       DACDTH010000104         6643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDTH010000104 DACDTH010000000
VERSION     DACDTH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.107"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..859)
                     /locus_tag="IWK54_002675"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IWK54_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IWK54_002676"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IWK54_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP9594733.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="IWK54_002677"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="IWK54_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="IWK54_002678"
     CDS             complement(2143..2373)
                     /locus_tag="IWK54_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP9594734.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="IWK54_002679"
     CDS             complement(2603..3421)
                     /locus_tag="IWK54_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9594735.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="IWK54_002680"
     CDS             3582..4271
                     /locus_tag="IWK54_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9594736.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="IWK54_002681"
     CDS             4285..5787
                     /locus_tag="IWK54_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9594737.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="IWK54_002682"
     CDS             5800..6276
                     /locus_tag="IWK54_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9594738.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="IWK54_002683"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="IWK54_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1033 c         1204 g         2234 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaacaccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DACDTH010000105         6552 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDTH010000105 DACDTH010000000
VERSION     DACDTH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.108"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            68..2743
                     /locus_tag="IWK54_002684"
     CDS             68..2743
                     /locus_tag="IWK54_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP9594739.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2925..3164
                     /locus_tag="IWK54_002685"
     CDS             2925..3164
                     /locus_tag="IWK54_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594740.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3180..4553
                     /locus_tag="IWK54_002686"
     CDS             3180..4553
                     /locus_tag="IWK54_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9594741.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4557..6191
                     /locus_tag="IWK54_002687"
     CDS             4557..6191
                     /locus_tag="IWK54_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9594742.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6354..>6552
                     /locus_tag="IWK54_002688"
     CDS             6354..>6552
                     /locus_tag="IWK54_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594743.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2300 a         1062 c         1317 g         1873 t
ORIGIN      
        1 aattatgaga ggttggaggt aatgtagtca tacaaataaa aattttttag aaagtgagtg
       61 gtaattaatg aaaatgaatc acacttcaat tcagaagcat ttacaactgt taaatacact
      121 ggggatttct tatgttaatt tgagaacttt ttctacatta aacaagaaaa ccgtttctca
      181 tattttttct actgcagatt ctcaagcaat tattaactat ttagaaaaac agaaattgtc
      241 tgaaggtaca gtaaacataa cttataatcc tattaaacaa gaggtgatta cacaaaaaaa
      301 gcattctgtg agagacaatg atattgaaaa aattcggttt gtatttattg atattgaccc
      361 taaaaggaaa gagggaagtg caacagatag cgaaaagaaa aaggcagaaa atgttatgga
      421 acaagtagaa cgttatttga aagaaaagag aatagaatca tttgtaaaag ttgatagtgg
      481 gaatggctac catatttttc ttcccataaa tgaacaacca aataatcacg agacgattct
      541 tactattcag aattttcttc aattacttca ccgaagattt ggagtagaag atgaagttga
      601 tattgatact tctgtttata atccttcaag attatgcaaa ttagttggta caccagcggt
      661 caaaggaaag tccacaaaag agcgtccgca tcgtttatct agtttccttt ctattcccga
      721 taacatagat agaagtaatt gttttaaaat tcttgaacag atagttgaag aaaattccat
      781 gatgtcaacc tctagagtaa aaagccatcc atcttttaga gaagtgttac cttttattca
      841 agcagactat aaacaatggt tagctcacta tcctgaatta acttatacca taaaagaggg
      901 cgatcataaa ggagttacct tacttatatt tgacgaatgt ccattaagaa aacacacgaa
      961 taattctaat ggttcttgtt tgtctgttac ggctgataat aagattcgat ttcattgtct
     1021 gcatgcttcg gataaaaata aagatattca agactttgta aaaaaatatc cgttgccaaa
     1081 acctgtaaat ccttctgtag aatcattaga atccaatcaa gaatattatt ttgaccagtt
     1141 taaactgaat caagaaggag tatttttggt gaaagaaggt caagaagaaa aagttgcttg
     1201 tccgatgttt attaagcaaa tacgcaaaaa tatcgaacaa aaagtcatta cacttgttct
     1261 tgcctattat gtcgatcatt gttgggatga aatagaaata gaaggtggat gtttacaaac
     1321 taataatttt aaacaattag caaaacatgg cttaatcatt aaagctagaa gagaagctga
     1381 agtcgttgac tttttacaga ttcaacggga gaatgctcca atcttttttg aacatgaatc
     1441 tcttggctgg aaaaaagaaa atgaagcaat gatttttcag ttacaagatt cttttggacg
     1501 tgaaattcct tctgtattaa ctaaaaactc atgctaccaa ttaacaacta atggtacaga
     1561 agcggaattt gacgcattgg ttcataagta tgttttagga acgaacttag aacttgcttg
     1621 gtcgattggt attgcttcta ttgtgttagg ctatttaaac gttacgaagg ctaatacttt
     1681 tttgagttta ctaattaacc tacagggcaa gagcacaacg ggtaaaacca ctacgttaca
     1741 ctttattgcc tctttatttg gaaatccaac tacggttctt agaaatatga atgctaccaa
     1801 taaagccatt ataaagctag catcaaacaa tcagggaggt gttccactaa tactagatga
     1861 gttaggagca gcaactcaaa ataactttag ctcgttgatc tatcaattag cttcaggaga
     1921 agaaaggcta agattagatt ctaattgtca gcttatgcca caagagactt tttctgtttt
     1981 gacccttacc tcttctgaag agcgtttgga aaattatttc aatgagaaat taactggttt
     2041 gaaagttcga tatatcgaat tttctgatat caagtttaca aaggatgcga atcacgcaga
     2101 gaaagttaaa agtctttctt ctaatacttt tggttggggt ataaagttat ttcttttgaa
     2161 actctttgaa caagggatta caaaaattga agcaactttt gtcgaagctc aaaaaaaact
     2221 atgggctgtt ttacctgaac attctttgaa gcaacgcatt ggtaataatc tagctttgat
     2281 ttatagtgga gccctcctaa ttcaagatat actgggctat cctttacgat tagagcatat
     2341 acagacggtg cttattaatt cgtacgaaca gttaattgta gatactagtt taggaagtga
     2401 gcaaacggta tttgaccgag tgaaaacatt gttggtgatg aatagccata aatttttaac
     2461 aaacagtgac aacaaacata cgcccagtac gatttgggga aaagtatcaa ttcaaaaaaa
     2521 tggggatatt aaggtaaata tttttcctat cccgtttgag gacttgttaa ggcgagaatt
     2581 tcaagtaaag gatctaacct atctttttag gcaattgctt gattcgggta atatgtgtgc
     2641 agaaaaaggg agaagaacga aacgtattca catgaatcgt caagttcttc caacatacga
     2701 gattctatta ccttcttcac tcaaatcgta ttttcgattg taatttagaa taaaccaaag
     2761 tatgaaagtg aggcaccatg ttatggaaat atttcttatt gatttagcaa aacaactgat
     2821 tacttatctg tatgatagcg agcaaaccca aacggaagta aatgaagctt gatctatgct
     2881 tcatttactt cattcaatca aacatgaaag aaaggaaaat tctaatgact aaaaataaaa
     2941 tgacactaaa agcagaggtt cttctgtata ttcaagaaca tttttcaaat caagcctttt
     3001 ttacaaaacc aatttacttg gatttcgaaa taagaggggt atctgctgga tctatcgggg
     3061 gtactcttca agcgttgaag aacgaaggat atttagaaaa tcactttgtg caacgttcgt
     3121 tcaatagaag agtcgtgaaa aaatggtatt tagttcattc atagagaggg gaatcaagca
     3181 tgcgtgtggc ttgttatgat cgaacgtctg tttatgaaga agcgaaaatt agtaatgacg
     3241 ctcaatatga tgcagtgaaa gagtatgttt tagagaaata tgaacaaatt ccccaacgat
     3301 tttcagacaa aggagaatca ggggaaaaag cggagcgtcc agcttttcga cagcttagag
     3361 cttgggtttg tgcgtcagaa gaagaggggg tgatctatgt ttggcgttat gatcgattgg
     3421 cacgtcaagc caaaatagcc ttagaattta ttgagttgtg ccaacaacat caggttcaag
     3481 tgtattcgat ttctgaacct cttcctgctt ctttctcaca cgcgtcaacg aataaactat
     3541 ttgtgcaatt attgttcact tttgcagaac aacaacgaaa cgtcattatt gagaatgttc
     3601 gtgccggact ttcctataaa aagcaacaac ggcaatattt gtcttctgcg gtaccatatg
     3661 ggtatcgttt agtgaatggg aaaatccaac aagaagtaca cgaagcaagg attgttcgct
     3721 atatttttca actttatttg accaaaaaat atggctacaa aaagctttgt caacgattga
     3781 cgcaacaaaa gcttttcttc cgcgaacgtc cttttcagcc ctatcatatt tatagtatcc
     3841 taaaaaatcc attgtactat ggcgaaatca aaggaggatc tttggggaag tatctaggaa
     3901 cctttgaacc gattctctcg aaagcaacct ttttacaagc ccaagagatt cgccaatcaa
     3961 ggtgtacagc taaaaaagat acgtatctgt acttattacg tcaaaaaatt cggtgtccct
     4021 tctgtggtcg tcatctgagt tcgaagtatc aatggaatac aaagaagacg aagacgcttc
     4081 attattatca ttgtaccgac cgaagctgcc aaggaatttt tcttcgggca gaaagggttg
     4141 agtcagaagt attggaaaag atacaggctt tttttcaata cgaaggcgtc tatcaagcct
     4201 tgattgccca attaacgcaa caattagccg aaaagcgaaa agatgaaaaa cgcaaagtaa
     4261 tccaacaaaa gaaacaagaa gcacaatgga ttaaggcatt tgaagagggc aattgttcac
     4321 tggaagaact caagcaacaa ctgcaagctc ttcaaaagcc gatgtctact tcttcgtttc
     4381 tttcactcca acagtgtgaa gcaaaagtga aagaatggct tcaactaaaa gacctctcta
     4441 gtcgacaatt attatgtgac tatgtttgtc ggattgaggt aacaaaggat aaaacagttt
     4501 gtgggattta cttccgacaa ctgacacaaa atattttaga aaggaacgaa tgatctatgg
     4561 aaatgataca aaccaaacga gtcgcattgt atattcgcgt gagtacacaa gaacaagctt
     4621 tagaaggaca tagtattcaa gcacaagaac aaaatggacg acagtttgcc gaacggatgg
     4681 gctatgaagt ggtagaagtt tatgccgatg aaggaatcag tgggaaatcc acaaaaaatc
     4741 ggttcgccta tcaacgaatg atgaacgatg ctcggaacca tcgttttgat ctactagtga
     4801 tttggaaatt gacccgtctc ggacgcaata tgttagatgt cttacaaaca gtcgaggaat
     4861 tattgcgata tgacgtagga ttacattcga tctcggaaca atttgacgtg accacttcga
     4921 caggtaaatt gatgttacaa ctccttggga gtttcggtga atttgaacgc aaccaaattg
     4981 cggagaatgt tcaaatgtca atgcaaagct tagtgaaaga taaaaagcgt tatgcaggag
     5041 gccgacgttt aggctatgtc agtggtattg acacgaatgg tcaaaaacaa ttggtcattg
     5101 aaccagaaga ggcgaaaatt gttcgtctga tctatgcaaa atatttaggc ggagcagggt
     5161 atcgagcgat tgccaatttt ttgaatcgtc aaggatatca aacagtcaaa aagaatgctt
     5221 tttctaccac ggcagtcaaa gatatcttac gcaataaaat ttatgcaggg tatttagaat
     5281 atgcgcgcta catcaattgg gaaaccaaac gacggaaggg gaagaatccg agaccgattt
     5341 tagtgaaagg aacccatgaa ccgattattt cggaagaaga ttatcaaaaa gtacaagaac
     5401 gccttgcttt agaaggaaaa caacccaaat ggaaccatac aggtgaaaat gtattgacag
     5461 gcttgttacg ttgtccagaa tgtggcgcac cgatggcagc gagtaatgtc acaaataccc
     5521 taaaggatgg cacaaagaaa cggattcgat attattcttg ttcagtcttt cggaataaag
     5581 gagcaagtgt gtgtcatgcc aattcgattc gtgccgaaca agcggaaaca tttgtcgccg
     5641 accgattaaa ggagatcgtt cagctaccag aagttcttcc ccgattggtg gcagcgttaa
     5701 atgaagaaat agtgcgacaa agccaaccat tggaacaaga actcgtggtg ttactggaac
     5761 gaaaggaaga attgaagact aaaattgaaa aatgggaagc tgctttggaa gatagtcctg
     5821 aattgtttcc gatgttaaaa gaccgcttag acgaattgac tgaaaaacga cgacagttgc
     5881 acattcgaga aaatgaaatc ttagggatct ttcaacaagg agagccgatt caagtcaaag
     5941 atgtccaacg gatattgact aatttggatc gttttcttgc ccagagtgag aagaagcaaa
     6001 tcaaagcctt gtatcgtacc tttatagaga aaataacctt tgatcctaac cataaggaga
     6061 tgttggaaat tacgatgaaa ttcacgcatg ccgtggtaca acaattgaat gagcaatatc
     6121 aaatagccgt atccaaaaca aaggatacgg ctatttttgt gttaaaaaca ccctttacgt
     6181 taacgatcta gtctaggtta tatcaaacaa cgttagaggg gcgctaacgc ttctctagcg
     6241 atttgaaatc gttagagaag tagttaggga ggtccttatc ataaataggg atatttatta
     6301 atgaatattt gaaatataat tgtatataag aattaaaaag agtggggata gatatgaaaa
     6361 agatgggtaa gtgtatttta tgtgaaaaat acactgaact tactaaagaa catgtaccac
     6421 ctaaaaaatc agggaatact ggaggtaaaa agaccagaac tggtaattta gatgatttcc
     6481 ttaagagcga ttttacaaaa ggtgattttc cgaaggggat taaaaggaaa cctcaaggaa
     6541 atgtttatta ta
//
LOCUS       DACDTH010000106         6303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDTH010000106 DACDTH010000000
VERSION     DACDTH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.110"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            334..714
                     /locus_tag="IWK54_002689"
     CDS             334..714
                     /locus_tag="IWK54_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9594744.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="IWK54_002690"
     CDS             696..1610
                     /locus_tag="IWK54_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9594745.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="IWK54_002691"
     CDS             1855..2544
                     /locus_tag="IWK54_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594746.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="IWK54_002692"
     CDS             complement(2665..2751)
                     /locus_tag="IWK54_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9594747.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="IWK54_002693"
     CDS             3427..4377
                     /locus_tag="IWK54_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9594748.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="IWK54_002694"
     CDS             4398..4931
                     /locus_tag="IWK54_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9594749.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5567..6100
                     /locus_tag="IWK54_002695"
     CDS             5567..6100
                     /locus_tag="IWK54_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594750.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DACDTH010000107         6287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDTH010000107 DACDTH010000000
VERSION     DACDTH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.111"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IWK54_002696"
     CDS             <1..177
                     /locus_tag="IWK54_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594751.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IWK54_002697"
     CDS             190..690
                     /locus_tag="IWK54_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9594752.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="IWK54_002698"
     CDS             787..2634
                     /locus_tag="IWK54_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP9594753.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="IWK54_002699"
     CDS             2637..3533
                     /locus_tag="IWK54_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9594754.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="IWK54_002700"
     CDS             3583..3972
                     /locus_tag="IWK54_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9594755.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="IWK54_002701"
     CDS             3959..>6287
                     /locus_tag="IWK54_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9594756.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DACDTH010000108         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDTH010000108 DACDTH010000000
VERSION     DACDTH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.112"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IWK54_002702"
     CDS             725..811
                     /locus_tag="IWK54_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9594757.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IWK54_002703"
     CDS             complement(932..1621)
                     /locus_tag="IWK54_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594758.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IWK54_002704"
     CDS             complement(1628..1906)
                     /locus_tag="IWK54_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594759.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IWK54_002705"
     CDS             complement(1866..2780)
                     /locus_tag="IWK54_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9594760.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IWK54_002706"
     CDS             complement(2762..3142)
                     /locus_tag="IWK54_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9594761.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IWK54_002707"
     CDS             complement(3592..3879)
                     /locus_tag="IWK54_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9594762.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IWK54_002708"
     CDS             complement(3899..4123)
                     /locus_tag="IWK54_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP9594763.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IWK54_002709"
     CDS             complement(4763..5296)
                     /locus_tag="IWK54_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9594764.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IWK54_002710"
     CDS             complement(5317..6159)
                     /locus_tag="IWK54_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9594765.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDTH010000109         6127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDTH010000109 DACDTH010000000
VERSION     DACDTH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.113"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IWK54_002711"
     CDS             <1..395
                     /locus_tag="IWK54_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594766.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="IWK54_002712"
     CDS             398..1228
                     /locus_tag="IWK54_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9594767.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="IWK54_002713"
     CDS             1244..2095
                     /locus_tag="IWK54_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9594768.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="IWK54_002714"
     CDS             2092..2451
                     /locus_tag="IWK54_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP9594769.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="IWK54_002715"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="IWK54_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="IWK54_002716"
     CDS             2624..2929
                     /locus_tag="IWK54_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594770.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="IWK54_002717"
     CDS             2929..3240
                     /locus_tag="IWK54_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594771.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3407..3823
                     /locus_tag="IWK54_002718"
     CDS             3407..3823
                     /locus_tag="IWK54_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9594772.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="IWK54_002719"
     CDS             3823..4491
                     /locus_tag="IWK54_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9594773.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="IWK54_002720"
     CDS             4488..4865
                     /locus_tag="IWK54_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594774.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="IWK54_002721"
     CDS             4862..5161
                     /locus_tag="IWK54_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594775.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5403..5819
                     /locus_tag="IWK54_002722"
     CDS             5403..5819
                     /locus_tag="IWK54_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9594776.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2262 a          922 c         1423 g         1520 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 gaattttaac agaattaggc agaagtcgcc catcctctat aggtgggtga tgaatgccgt
     6061 tcggtatgag ggttaccgtt ggtaactcga agaccgacaa ctaaggcgta ttgccttttt
     6121 attttgt
//
LOCUS       DACDTH010000110         5955 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDTH010000110 DACDTH010000000
VERSION     DACDTH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.115"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            44..1051
                     /locus_tag="IWK54_002723"
     CDS             44..1051
                     /locus_tag="IWK54_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9594777.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1063..2067
                     /gene="neuB"
                     /locus_tag="IWK54_002724"
     CDS             1063..2067
                     /gene="neuB"
                     /locus_tag="IWK54_002724"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP9594778.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2064..3212
                     /gene="neuC"
                     /locus_tag="IWK54_002725"
     CDS             2064..3212
                     /gene="neuC"
                     /locus_tag="IWK54_002725"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP9594779.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3185..3862
                     /locus_tag="IWK54_002726"
     CDS             3185..3862
                     /locus_tag="IWK54_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9594780.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3852..4994
                     /locus_tag="IWK54_002727"
     CDS             3852..4994
                     /locus_tag="IWK54_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP9594781.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            5007..>5955
                     /locus_tag="IWK54_002728"
     CDS             5007..>5955
                     /locus_tag="IWK54_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9594782.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYII"
BASE COUNT         2214 a          759 c         1152 g         1830 t
ORIGIN      
        1 gaatcgaatt atagatgact ataattcata ggaggagata taagtgttta aaggaaaaac
       61 aatattagta actggcggaa ctggttccat tggaagtgaa attgttcgtc agcttttaaa
      121 atatgaacca aaagctatca gaatttatag tagaggtgaa gaaaaacaat tttatatgca
      181 acaagagctc tcaaatcaca ctaatttgag atttttaatt ggtgatatca gagactctca
      241 aaggattgat tatgcatgtc gtgatgtgga tattattttt catgctgccg caatgaaaca
      301 cgttccagca agtgaatata atcctatgga agctgtaaaa acaaatgtga tgggaactca
      361 aaatgtaata gatgcagcga tcaaaaataa tgtaaaacat tttgtaggta tcagtacaga
      421 taaagtagtg agtcctacta acaccatggg tgcgacaaag ttattgagtg aaaaattaat
      481 aatagctgcc aatcagtata aaggaaaaac agaaacaata ttttcttgtg taaggtttgg
      541 aaatgtcatg gggtcaagtg gatctgttat tcctcttttt agtaaacaaa taaaaaacaa
      601 taaagatatt actttgacag atgttgatat gacacgattt atgatgtcaa taccggatgc
      661 tgcaagatta gttctgtctg ctgctatgaa ttcaaaattt ggagaaacat atgtcttaaa
      721 aatgccggca atcaatattt taactttagc tcaatcatta ataaaaatat ataatgatac
      781 acatgatgaa aaatatagag gtgaaataaa agttgtaggt atcaggccag gagaaaaatt
      841 atatgaagag ctaatgacta cagacgaaat tgaacgctca tatgaaaatg ataaaatgta
      901 tatcattcca ccacactatc atgctactga cgaactgtac aaagattatg aaaaaattaa
      961 aatcactgca tactcttcag atgcaacaga attaatgagt ggacaggaat ttattagaat
     1021 cgcaaaagag aataatgtcg tgacttggta aggagtttta tgatgaaatc agaagtatat
     1081 gtaattgcag aaattggtgt aaaccataat ggaagtctag atttagcttt agaaagtatt
     1141 gataaagcta aagaatgtgg agctaacgcg gttaaatttc aaacatttaa aacgaataag
     1201 ctagtttcca aaaaggctcc aatggcaaaa tatcagaagg ataatcttaa agaggacagt
     1261 tctcaatttg aaatgctaaa aaaattagaa ttatctcaat ctgattttat tagaataaaa
     1321 gattattgta tacaaaataa aattgatttt ttgtctactc cttttgatga agaaagtgca
     1381 gactttctaa aacaaattgg tgtacaagga tttaaaattg gctcaggaga tttaacaaat
     1441 ttaccttttc ttagaaaaat agatatgtat gggttgccag ttttattatc tacaggtatg
     1501 gctgatattc atgaggtcag tgaggctgta gattgtttca tagatagtcc agttactatt
     1561 ttacattgta cttcaaatta tccggctagt tctgaagata ttaatttatt ggcgattaac
     1621 acattaaaag aaatgttcgg gatacccgtt gggtactccg atcattcatt gggatatgat
     1681 gttggtattt gtgctgttgc gatgggtgct aaggtaattg agaaacattt taccttggat
     1741 aaggaactac ctggtccaga tcataaagct tctttagatc ctcaggaatt tgcagatttt
     1801 gtaaagcata ttagaaacac tgaaatattt ttaggtgatg gtgttaagcg agcaatgcct
     1861 tctgaactta gtacaaaaga agtatcaaga aaaagcctag tggtcactga gaatttaaat
     1921 gtgggagata ttcttacaga gaaatctctt acaataaaac gtcctggaaa tggaatagct
     1981 cctaagtttt tatatcaata tattggcaaa aaagttaaaa gaccaataag tgcagatagt
     2041 gttctgcttg aggaagatgt tgaatgaaaa aaatacttat agtaagtgga acacgagctg
     2101 attatggaat atataaacct gttgctgaga atttactaca agataattgg gatgtatcat
     2161 ttgttgtttc tggaatgcat ttatcccata aatatggatt tactaaagaa ttgatcttag
     2221 ctgacggatt tagaattagt ggagaagttc aaagtttgtt tttagagaac aataagggaa
     2281 acatggctcg gtctgtggca ttagagataa atggtttcac aaatatattt gaacaaacta
     2341 gccctgattt tgtattatta ctaggggata gaggagaaat gttggcagct gcgatagctt
     2401 gtttgtatct tggaatcaaa acagcacata ttcacggagg cgaagtgtct ggtactgtag
     2461 atgaatcaat aagacatgcc ataacgaaaa tagcatctgt tcattttgct gctacggaaa
     2521 aaagcaaaga gcgtttaata aaaatggggg aagattcttg gagagttttt cccgtcggag
     2581 ctcctagaat agatactatt ttaaataata gattacctag ttttagagat ataaaagaaa
     2641 aatatcatct aaaatttgaa aagaaaaaat acgttttgca agtatttcat ccagttgtaa
     2701 ccgaactaga aaatatagaa aaacaaattt tagctttgat caattcaatg gaacaacaat
     2761 ctcaaccaat tatttgcatt ttacctaata gtgatgctgg tagcgaaatt atcagaaaaa
     2821 tgtaccagaa atatttaaaa acggatatta tttatattac gaatttagag ccggaggaat
     2881 acctgactat attaaaagag tgcaaattta tgatcggaaa ttcttcttct gggataattg
     2941 aagctgcatc attcaaaata ccagtaataa atttaggaac cagacaaaat ggacgagaac
     3001 aatctaaaaa tattataaat gcaaatctaa atgaaaatga aattgaaact gctttgaata
     3061 aaatacaaga tcctcagttt ataaaaagct tgaaaaatgt taagaacatc tacggtgacg
     3121 gcaacagttc tagaagaata acaaaaacat tgaatcaaat attgaatgat ggggaagata
     3181 ttaagtggat tcaaaaacaa atagcttatt aaaatatact agtgatccat acattattat
     3241 tggggctggc ggacacgcaa aagtcattat tgaaacctta agattagaaa ataagttaat
     3301 atatggattt tttgatgata agcaaaataa aatgcaagat ctgaaatatc ttgggaaaat
     3361 aaacgaattg aatagctggg cggatagcaa aaaaaaaatc tataaattta ttgtagctat
     3421 tggtgactgt gtagtaagaa aaaagattat aaaaaaattg aatatgccaa gtgagcaata
     3481 tggaatagct gttcatccaa gtgctatagt ggcaaaagaa atttcaattg gttatggaac
     3541 tgttattttt gcgaatgctg taattaaccc agatgctgta attggagagc atgctataat
     3601 aaacacaggt agcattgttg agcatgattg tagaattaat aactatgttc atttatctcc
     3661 aggtgtatgc ttagcgggtg gagtacatgt aggtgtagga actcaaatag gaattggttc
     3721 tcaatgtatc cagatgaaga aaattggatc ttggtgtatg ataggcgcag ggagcactat
     3781 tgttaaggat attccctcac attccttagc ttatggcaat ccagctaaaa ttaagaaaga
     3841 aggaattaat tttgaattct agaattttat tatcaagtcc tcatatggga ggaacagaag
     3901 aaaaatatgt tgctaaggct tttaaagata attggatagc gccattaggt gaaaatgtgg
     3961 ataaatttga agaaagtatt acaaaatata ctggttctaa gggcggagct tctgcgttaa
     4021 gttcaggtac agctgccata catcttgcct taagactgct taatgttgga aaaaacgata
     4081 ttgtctttgc tccttcattt acttttatag ctacagtaaa tcctatttta tatcaagggg
     4141 ctgtaccagt gttcattgac tcagaaccta atacatggaa tatgagccct gttgcactag
     4201 agagcgcatt agaagaagct gaaagaaaca accaattacc taaggcaatc atcgtagtga
     4261 atttatatgg tcaaatggct gattatgaac gaattttagc tattgcaaac caatttaatg
     4321 ttccagtgat agaagatgca gcggaatcgt taggttctac ttataaggga atttcgtcag
     4381 gcttatttgg gacaattggg atctattcat ttaacggtaa taagatcatc acaacctcag
     4441 gtggaggagc tctagtgtct agtgataaaa aatatgttga gcattcaaga tttttagcaa
     4501 cacaagcaag agatcaagca ccgtattatc aacatagcga aataggttat aattaccgtc
     4561 taagcaatat ttctgccggt atcggacgtg gacaaatgga agtattgaat gatagagtag
     4621 ctaaacgaag agaaattttc aatcgatata aggaagcttt ttctggaatt gatagaattg
     4681 aaatgatgcc agaagtggat ggcatggtgc caaatcgttg gttgtctact ttaacgatca
     4741 atgattataa ttctattgat caagtagtta acgtaataaa taaactaaat gaaaaaaaca
     4801 ttgaaacaag agctttgtgg aaaccactac atttgcaacc agtatttcaa ggggtaaaaa
     4861 tgtattacca tgataataat cttagtgtgt cagaaaagtt atttaatagc ggcttatgct
     4921 taccttctgg aagtaatatg acagatagtg aacaagaatt tgttattgat gagtttaaaa
     4981 aaatattcaa gtaggtgtga tattttatga acataggaat tattggttta ggttccatgg
     5041 ggaaaagaag acttagactc attcaagaac actttaatga tattttacta tgtggtgttg
     5101 atactaatga ggcgagacga aaggaagttg aaaaggaatt tggaatacca acttataata
     5161 gtctaacaga ggcaaaaaat ttgtttgaaa taaattcagt ttttgtttgt acatccccta
     5221 taacccatga atttattata aaagaggctt taaatttaga ttgtaatatc tttacggaaa
     5281 tcaatttatt aaatgaatat tatgatgaaa taatcgaagt agctaaaaag aaagatagat
     5341 tactttatct atcttctaca tttttgaaga gaaaagaaat tcaatatatt aagtctaaag
     5401 tgacgcataa taaaaaaatt tcttatcaat accatgtagg acagtattta cccgactggc
     5461 atccttggga agattatcgc aatttttttg tttcaaacaa aaaaactaat gggtgtagag
     5521 aaatattcgc tattgaatta ccttggatta tagatacatt tgggaaagtt aaatcgttta
     5581 ctgtaatgac ccagaaatta tccaatcttg atattgatta ttcggacagt ttcactctta
     5641 ttattgagca tgaaactggt atagttggaa ctttaagtgt agatgttgtt tcaagagtag
     5701 ctaaaagaaa tttaactata attggtgaaa agattttaat agaatgggaa ggtaacccgg
     5761 attctctatt ttcatggaat gaagaaactt ctagtatgac agatattcaa ttgtacgatg
     5821 aggttatgca tgataataat tattcacata ctattattga agatgcatat ttagaggaga
     5881 ttaaagaatt tttcacgctt ttggaaaaga aaatggaacc ccaatatact tttgaaaaag
     5941 ataagtatat tatta
//
LOCUS       DACDTH010000111         5935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDTH010000111 DACDTH010000000
VERSION     DACDTH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.116"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(170..3319)
                     /locus_tag="IWK54_002729"
     CDS             complement(170..3319)
                     /locus_tag="IWK54_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9594783.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="IWK54_002730"
     CDS             complement(3333..4550)
                     /locus_tag="IWK54_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9594784.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..>5935)
                     /locus_tag="IWK54_002731"
     CDS             complement(4540..>5935)
                     /locus_tag="IWK54_002731"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9594785.1"
                     /translation="RELLSDEESKEDLIATIVDILGYSISPEYLFNVLADQAKQATFQ
                     LNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGH
                     DGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTM
                     GSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNK
                     DWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYH
                     LKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVL
                     KKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIK
                     ENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENE
                     AWLQGALEVFKHEQ"
BASE COUNT         1623 a         1261 c          993 g         2058 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt cacga
//
LOCUS       DACDTH010000112         5904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDTH010000112 DACDTH010000000
VERSION     DACDTH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.117"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..986)
                     /locus_tag="IWK54_002732"
     CDS             complement(<1..986)
                     /locus_tag="IWK54_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAP9594786.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1138..1833
                     /gene="vanR"
                     /locus_tag="IWK54_002733"
     CDS             1138..1833
                     /gene="vanR"
                     /locus_tag="IWK54_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9594787.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1811..2965
                     /locus_tag="IWK54_002734"
     CDS             1811..2965
                     /locus_tag="IWK54_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9594788.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3180..4148
                     /locus_tag="IWK54_002735"
     CDS             3180..4148
                     /locus_tag="IWK54_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9594789.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4141..5172
                     /gene="vanA"
                     /locus_tag="IWK54_002736"
     CDS             4141..5172
                     /gene="vanA"
                     /locus_tag="IWK54_002736"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9594790.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5178..5786
                     /locus_tag="IWK54_002737"
     CDS             5178..5786
                     /locus_tag="IWK54_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP9594791.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1782 a         1149 c         1335 g         1638 t
ORIGIN      
        1 tttccgataa ccgtgtattg aaaggcatct tcgaagccat tatccaatat ttcgcaggct
       61 tttgtatatt taggctggtc gatatattca ccgactaagg cattcttagc tgttcgagcg
      121 agttcaatat ccgtaaactt aaagatcgct tttactgctt ctctaaacgg ttttgaattc
      181 ttttttggaa tggaactgaa gatgtttctt aaaaaatgga cctggcatct ctgccaactt
      241 gcgttggtaa atgacttacg aatcgcagac actaggcctt tatgggcatc agaaatgatg
      301 agttctgtcc cctttaggcc gcgttctttt aagtattcaa agaagatgga ccatgtgtca
      361 tcactttctt cattttgaat catgaagcca atgatttcac ggtcgccacc ttctgttatc
      421 ccgatcgcaa tatggcagct tttagaaagc actcgatggt cctcacggac ttttatgtag
      481 agaacatcag tcatcagata aggataattc gtacctgaga gtgaacggtt ctgccattcg
      541 ttgaccatcg ggtctaactg ctcagtcagg ctagaaacaa aagatttcga tacagatttt
      601 ccacatagct cttcaacaat ctttgaaact ttacgtgtcg aaacgcccga aacatacatc
      661 tcaagcattg aagcgagcag tgccttttca tttcgctgat aacgctcaaa caccgtcggt
      721 gaaaattcac catcacgtgt tctaggcact tttaattcga gtgtacccac acgagtcgta
      781 aagtctcgct cataatagcc atttctttga ctctgacgac tttctgaacg ttcatagtca
      841 tcggcttgaa tatattctgt tcgttgattt tccatcaatt ggttgaaaac agtggttaaa
      901 atatttttag aaacatcatc ttttaccgaa tgttcaataa tactttgaac ctcttcgttg
      961 ttcagtgtaa aatgtacttg ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa
     1021 aacattgtac cgtaaaaggg ctgttacatg gccttttatc ttttacacaa ttatatggac
     1081 tttatcctat aatctgataa attattaagg gcaaacttat gtgaaagggt gataactatg
     1141 agcgataaaa tacttattgt ggatgatgaa catgaaattg ccgatttggt tgaattatac
     1201 ttaaaaaacg agaattatac ggttttcaaa tactataccg ccaaagaagc attggaatgt
     1261 atagacaagt ctgagattga ccttgccata ttggacatca tgcttcccgg cacaagcggc
     1321 cttactatct gtcaaaaaat aagggacaag cacacctatc cgattatcat gctgaccggg
     1381 aaagatacag aggtagataa aattacaggg ttaacaatcg gcgcggatga ttatataacg
     1441 aagccctttc gcccactgga gttaattgct cgggtaaagg cccagttgcg ccgatacaaa
     1501 aaattcagtg gagtaaagga gcagaacgaa aatgttatcg tccactccgg ccttgtcatt
     1561 aatgttaaca cccatgagtg ttatctgaac gagaagcagt tatcccttac tcccaccgag
     1621 ttttcaatac tgcgaatcct ctgtgaaaac aaggggaatg tggttagctc cgagctgcta
     1681 tttcatgaga tatggggcga cgaatatttc agcaagagca acaacaccat caccgtgcat
     1741 atccggcatt tgcgcgaaaa aatgaacgac accattgata atccgaaata tataaaaacg
     1801 gtatgggggg ttggttataa aattgaaaaa taaaaaaaac gactattcca aactagaacg
     1861 aaaactttac atgtatatcg ttgcaattgt tgtggtagca attgtattcg tgttgtatat
     1921 tcgttcaatg atccgaggga aacttgggga ttggatctta agtattttgg aaaacaaata
     1981 tgacttaaat cacctggacg cgatgaaatt atatcaatat tccatacgga acaatataga
     2041 tatctttatt tatgtggcga ttgtcattag tattcttatt ctatgtcgcg tcatgctttc
     2101 aaaattcgca aaatactttg acgagataaa taccggcatt gatgtactta ttcagaacga
     2161 agataaacaa attgagcttt ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt
     2221 aaaacggact ctggaaaagc gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga
     2281 cgttgttatg tacttggcgc acgatattaa aacgcccctt acatccatta tcggttattt
     2341 gagcctgctt gacgaggctc cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat
     2401 cacgttggac aaagcgtatc gactcgaaca gctaatcgac gagttttttg agattacacg
     2461 gtataaccta caaacgataa cgctaacaaa aacgcacata gacctatact atatgctggt
     2521 gcagatgacc gatgaatttt atcctcagct ttccgcacat ggaaaacagg cggttattca
     2581 cgcccccgag gatctgaccg tgtccggcga ccctgataaa ctcgcgagag tctttaacaa
     2641 cattttgaaa aacgccgctg catacagtga ggataacagc atcattgaca ttaccgcggg
     2701 cctctccggg gatgtggtgt caatcgaatt caagaacact ggaagcatcc caaaagataa
     2761 gctagctgcc atatttgaaa agttctatag gctggacaat gctcgttctt ccgatacggg
     2821 tggcgcggga cttggattgg cgattgcaaa agaaattatt gttcagcatg gagggcagat
     2881 ttacgcggaa agcaatgata actatacgac gtttagggta gagcttccag cgatgccaga
     2941 cttggttgat aaaaggaggt cctaagagat gtatataatt ttttaggaaa atctcaaggt
     3001 tatctttact ttttcttagg aaattaacaa tttaatatta agaaacggct cgttcttaca
     3061 cggtagactt aataccgtaa gaacgagccg ttttcgttct tcagagaaag atttgacaag
     3121 attaccattg gcatccccgt tttatttggt gcctttcaca gaaagggttg gtcttaatta
     3181 tgaataacat cggcattact gtttatggat gtgagcagga tgaggcagat gcattccatg
     3241 ctctttcgcc tcgctttggc gttatggcaa cgataattaa cgccaacgtg tcggaatcca
     3301 acgccaaatc cgcgcctttc aatcaatgta tcagtgtggg acataaatca gagatttccg
     3361 cctctattct tcttgcgctg aagagagccg gtgtgaaata tatttctacc cgaagcatcg
     3421 gctgcaatca tatagataca actgctgcta agagaatggg catcactgtc gacaatgtgg
     3481 cgtactcgcc ggatagcgtt gccgattata ctatgatgct aattcttatg gcagtacgca
     3541 acgtaaaatc gattgtgcgc tctgtggaaa aacatgattt caggttggac agcgaccgtg
     3601 gcaaggtact cagcgacatg acagttggtg tggtgggaac gggccagata ggcaaagcgg
     3661 ttattgagcg gctgcgagga tttggatgta aagtgttggc ttatagtcgc agccgaagta
     3721 tagaggtaaa ctatgtaccg tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc
     3781 atgtgccgct caatacggat acgcactata ttatcagcca cgaacaaata cagagaatga
     3841 agcaaggagc atttcttatc aatactgggc gcggtccact tgtagatacc tatgagttgg
     3901 ttaaagcatt agaaaacggg aaactgggcg gtgccgcatt ggatgtattg gaaggagagg
     3961 aagagttttt ctactctgat tgcacccaaa aaccaattga taatcaattt ttacttaaac
     4021 ttcaaagaat gcctaacgtg ataatcacac cgcatacggc ctattatacc gagcaagcgt
     4081 tgcgtgatac cgttgaaaaa accattaaaa actgtttgga ttttgaaagg agacaggagc
     4141 atgaatagaa taaaagttgc aatactgttt gggggttgct cagaggagca tgacgtatcg
     4201 gtaaaatctg caatagagat agccgctaac attaataaag aaaaatacga gccgttatac
     4261 attggaatta cgaaatctgg tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa
     4321 aacgacaatt gctattcagc tgtactctcg ccggataaaa aaatgcacgg attacttgtt
     4381 aaaaagaacc atgaatatga aatcaaccat gttgatgtag cattttcagc tttgcatggc
     4441 aagtcaggtg aagatggatc catacaaggt ctgtttgaat tgtccggtat cccttttgta
     4501 ggctgcgata ttcaaagctc agcaatttgt atggacaaat cgttgacata catcgttgcg
     4561 aaaaatgctg ggatagctac tcccgccttt tgggttatta ataaagatga taggccggtg
     4621 gcagctacgt ttacctatcc tgtttttgtt aagccggcgc gttcaggctc atccttcggt
     4681 gtgaaaaaag tcaatagcgc ggacgaattg gactacgcaa ttgaatcggc aagacaatat
     4741 gacagcaaaa tcttaattga gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg
     4801 ggaaacagtg ccgcgttagt tgttggcgag gtggaccaaa tcaggctgca gtacggaatc
     4861 tttcgtattc atcaggaagt cgagccggaa aaaggctctg aaaacgcagt tataaccgtt
     4921 cccgcagacc tttcagcaga ggagcgagga cggatacagg aaacggcaaa aaaaatatat
     4981 aaagcgctcg gctgtagagg tctagcccgt gtggatatgt ttttacaaga taacggccgc
     5041 attgtactga acgaagtcaa tactctgccc ggtttcacgt catacagtcg ttatccccgt
     5101 atgatggccg ctgcaggtat tgcacttccc gaactgattg accgcttgat cgtattagcg
     5161 ttaaaggggt gataagcatg gaaataggat ttactttttt agatgaaata gtacacggtg
     5221 ttcgttggga cgctaaatat gccacttggg ataatttcac cggaaaaccg gttgacggtt
     5281 atgaagtaaa tcgcattgta gggacatacg agttggctga atcgcttttg aaggcaaaag
     5341 aactggctgc tacccaaggg tacggattgc ttctatggga cggttaccgt cctaagcgtg
     5401 ctgtaaactg ttttatgcaa tgggctgcac agccggaaaa taacctgaca aaggaaagtt
     5461 attatcccaa tattgaccga actgagatga tttcaaaagg atacgtggct tcaaaatcaa
     5521 gccatagccg cggcagtgcc attgatctta cgctttatcg attagacacg ggtgagcttg
     5581 taccaatggg gagccgattt gattttatgg atgaacgctc tcatcatgcg gcaaatggaa
     5641 tatcatgcaa tgaagcgcaa aatcgcagac gtttgcgctc catcatggaa aacagtgggt
     5701 ttgaagcata tagcctcgaa tggtggcact atgtattaag agacgaacca taccccaata
     5761 gctattttga tttccccgtt aaataaactt ttaaccgttg cacggacaaa ctatataagc
     5821 taactctttc ggcaggaaac ccgacgtatg taactggttc ttagggaatt tatatatagt
     5881 agatagtatt gaagatgtaa ggca
//
LOCUS       DACDTH010000113         5871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDTH010000113 DACDTH010000000
VERSION     DACDTH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.118"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(525..1028)
                     /locus_tag="IWK54_002738"
     CDS             complement(525..1028)
                     /locus_tag="IWK54_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9594792.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1387..2412
                     /locus_tag="IWK54_002739"
     CDS             1387..2412
                     /locus_tag="IWK54_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9594793.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2735..3613
                     /locus_tag="IWK54_002740"
     CDS             2735..3613
                     /locus_tag="IWK54_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594794.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4089..4205
                     /locus_tag="IWK54_002741"
     CDS             4089..4205
                     /locus_tag="IWK54_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9594795.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5133..5471
                     /locus_tag="IWK54_002742"
     CDS             5133..5471
                     /locus_tag="IWK54_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594796.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5565..5795
                     /locus_tag="IWK54_002743"
     CDS             5565..5795
                     /locus_tag="IWK54_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594797.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1994 a          884 c         1076 g         1917 t
ORIGIN      
        1 aataacatat ctcattttac gcttttgtta ttttatactt aaaaaataac gacacagaaa
       61 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
      121 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      181 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      241 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      301 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      361 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      421 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      481 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      541 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      601 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      661 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      721 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      781 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      841 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
      901 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
      961 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
     1021 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
     1081 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
     1141 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
     1201 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
     1261 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
     1321 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
     1381 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
     1441 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
     1501 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
     1561 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
     1621 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
     1681 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
     1741 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
     1801 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
     1861 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
     1921 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
     1981 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
     2041 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
     2101 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
     2161 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
     2221 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
     2281 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
     2341 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
     2401 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
     2461 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa
     2521 aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat
     2581 gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat
     2641 attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata
     2701 ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg
     2761 aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc
     2821 caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga
     2881 gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga
     2941 caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca
     3001 aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat
     3061 gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga
     3121 ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga
     3181 gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg
     3241 cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta
     3301 cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta
     3361 cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat
     3421 ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt
     3481 cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt
     3541 acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat
     3601 tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag
     3661 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
     3721 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
     3781 ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg
     3841 ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt
     3901 ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag
     3961 tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac
     4021 aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg
     4081 tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata
     4141 tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa
     4201 ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg gctattttca
     4261 ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca tttttaacat
     4321 atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt ccagttatat
     4381 agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt agttattttt
     4441 caattccctt tcaacctctt attttgacca gtagtattga tcactttacc ttttaacacc
     4501 tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat aatcaaaggt
     4561 tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt gcaaataacc
     4621 attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca gctcactcca
     4681 tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga aaaagttaac
     4741 tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac tattgctaga
     4801 ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt tgtgtattgg
     4861 aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca ctattacaag
     4921 tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa tgaatagttg
     4981 tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc aatttgtgat
     5041 tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt gtagtgttta
     5101 ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca agtgaggaac
     5161 atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat attgttccgg
     5221 agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata ggatggtata
     5281 tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca gtaggcttag
     5341 agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact gatgaaagtg
     5401 acgagactga gatcatttta acggattact caaaaattgc tactgataaa aagtatatat
     5461 tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa atttactaga
     5521 tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg gtagaaaagg
     5581 gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa aatgagtgga
     5641 aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt tacagagaag
     5701 gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta gctgaagtag
     5761 aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat gaaaaatttt
     5821 gacacatatt atgttattgt tttttcgata tctttgctgt taatattact a
//
LOCUS       DACDTH010000114         5809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDTH010000114 DACDTH010000000
VERSION     DACDTH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.119"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            450..1604
                     /locus_tag="IWK54_002744"
     CDS             450..1604
                     /locus_tag="IWK54_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP9594798.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="IWK54_002745"
     CDS             1598..2020
                     /locus_tag="IWK54_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594799.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="IWK54_002746"
     CDS             3143..4075
                     /locus_tag="IWK54_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9594800.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="IWK54_002747"
     CDS             4092..4607
                     /locus_tag="IWK54_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9594801.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="IWK54_002748"
     CDS             4903..5184
                     /locus_tag="IWK54_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594802.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="IWK54_002749"
     CDS             5186..5602
                     /locus_tag="IWK54_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594803.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DACDTH010000115         5807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDTH010000115 DACDTH010000000
VERSION     DACDTH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.120"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IWK54_002750"
     CDS             <1..888
                     /locus_tag="IWK54_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP9594804.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(913..1170)
                     /locus_tag="IWK54_002751"
                     /pseudo
     CDS             complement(913..1170)
                     /locus_tag="IWK54_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1186..1836)
                     /locus_tag="IWK54_002752"
     CDS             complement(1186..1836)
                     /locus_tag="IWK54_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594805.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(1847..3019)
                     /locus_tag="IWK54_002753"
     CDS             complement(1847..3019)
                     /locus_tag="IWK54_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9594806.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(3038..3910)
                     /locus_tag="IWK54_002754"
     CDS             complement(3038..3910)
                     /locus_tag="IWK54_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9594807.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3932..4852)
                     /locus_tag="IWK54_002755"
     CDS             complement(3932..4852)
                     /locus_tag="IWK54_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594808.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(4852..5121)
                     /locus_tag="IWK54_002756"
     CDS             complement(4852..5121)
                     /locus_tag="IWK54_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594809.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(5118..5498)
                     /locus_tag="IWK54_002757"
     CDS             complement(5118..5498)
                     /locus_tag="IWK54_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP9594810.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1689 a         1187 c          950 g         1981 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc accaaaaagg caagtgcttc tttgtaggca ttttgactgt atttttttga
      961 taaagcatga caaatttctt gataaaatag ttgactgcct accgaatacc gattattgtg
     1021 tatccgccac aaaacgaaca cactgatttg aatcgacggg cgtaaaaaag aaaaattctt
     1081 tccataagaa aaagaacgtc cttcttgggt ttcttttgct tgttctgttg gtacttcttg
     1141 tacctctctt attgattctt gtctctccat aaccttctcc ttttcttatt cttctaaaag
     1201 ctgttgatta aagctacttc ccaaattttg cataaacgtg attttctttg acgctggatc
     1261 aaaggttacg tcaaaaatat tcatggcatt attccaggaa ctatcaccaa ttttattgcg
     1321 atacgacacc acaacgagcc cttggatagt cgctttttgt gtgcttttta aataaacaga
     1381 acatttctct aattcagaag acaccccgtt tgaatcacca taggaaacgc cttctttgaa
     1441 atattgcttc atcagtttgt cagaaagata tggtgcacaa ttgttttttc gttcttgaaa
     1501 agaatcaggc tcataattaa acagaacctc gaacgcttct gtcactgttt tttgaaaggt
     1561 ttgaatgttt cctttttctg ttgaatcatt gtttttcaat tcttcatttg ctttttttaa
     1621 cgtcgtgacc tcgctttcta cttgttgaag ttgttctttg agtgacgtta caaggtcttc
     1681 ctgtcctttc ttctcttctt ggaacttgtt tatctccacc gtcagtttct cttcctttgc
     1741 tgttagctgt agcttttcat gaataagaaa aaatgtactg cctacaccaa ataagaacaa
     1801 tccgcctaca caaacataaa taaatcgttt cttcatttga cacctcttat ttctttatcg
     1861 gtcgagcaaa acaaatatca ccaccattaa gcaaataccc aaaggataaa ttgacttgcc
     1921 cttcacctac accgttagta ttcattcccc cttgttcgat tactttcgta gaaagtccat
     1981 gccaatcttc cgctaaaaca gctgtgtgtc catcatttcc cacaccagaa cctaagttca
     2041 caatgataat atcgccagct tttgcctcgc tctcaggtat ttcttgtaag tattgcttac
     2101 ttcctcttgc gtcgctcgtc attgagccgg taaaccaccc aacgttcgca ggcgttttat
     2161 accctgcttt cgttaaaaca agccacacaa agctcgaaca atcggcatag ccatttttat
     2221 ctggattttc gacactacca aattgtgccc gttgcggttg tgaataatga aaataaccaa
     2281 tcagactttt tgctacgttt aatacattac tatcggaaga actgcttgtt cctgttgttt
     2341 ctagacaata atcggtcaag tctttacttc ctgcgtttgc cccattttct aatgtaattg
     2401 aagcgacggg atcactgtca ttctttccta gttttgcgta ccattgattc gcaaaagtaa
     2461 tacgattccc catcattggt atccctgcac gctcaaattt cacttgccac tgttctgttg
     2521 cttcctctac gctttttgct tgtaatgcaa cttgtgaagc tttttctgtt gtgtttagtt
     2581 catgaatcaa aaactcaact tgtacaccta aatctgacca ctctttgttt cgctctttgg
     2641 caaaattttt caaagcagtc gttcgccctg ccgtccattg tgcaatccca tgcccagata
     2701 atggatcgtt tggtcgttca atcgctgtgg gactcatact cgactcttgt tgaaaattgc
     2761 ctaacatacc cgctagaccc tctaatgtcg tatttggaat atgtttttgt acttcctttg
     2821 caatttgaag cccgtttgtc tccatgcctt ggttcgttaa tgagatagta cttgcgacaa
     2881 tttcggttgg actacagtcg atttcttcct cttcagaacc actactaatc aaaagtatca
     2941 taaacagaaa accaaacata catagtaata gtgtgggtag acaaccgaac ccacaccata
     3001 aaagaagttt tttcttcatg tttgatcctt cattcgctta gttcattttt tgtgttgctt
     3061 ttcttttgcg ccaaaaagca aaaccgcttc ctgaagccaa taatccacca agtgctgtcg
     3121 caacgagaga cacttcttcg ccagtcttag gcaatacttt catttttcca tcagaccctt
     3181 tcaccgcaat atttccgctt ggaagttgtt cgaccttgtc tgaaatgggc gtcaagccat
     3241 ttcctgattc tttataagcg acaccatcaa cgacggagac aattttttct cctgaagctg
     3301 tttcaattgg tgtacttggt gttggaatat gggttaattc agacgcatta ttggtttcaa
     3361 tttgaacatt ttgtccacct gtttgttggc tgccaatact tccatctggt tttactacga
     3421 cgtcctgtac tggaggcgta gtgttcgtcc ccccattatt ggtaggtggt gtcgttggag
     3481 gagtcgtatt tccttcattg cctcctggat tcgacggctt ttctggtgtc tctgtattac
     3541 caccatttcc tgggttttcc tcaacgggtg gaacttcagg tgttccttcc cctccgccag
     3601 tatctggatt ttctggtact tcaggcgttt ctgtgcctcc acctgtatct ggattttctt
     3661 ccgttggtgg aatctctggg gtttcaggag tttctggtat ttcgggaatt tctggggttt
     3721 cttctcctcc atctgttggt ggtaaaacag gttcttcagg aacttcaggt gtttcttccg
     3781 ttggtggaat aatttctgtc ggtggtacac ttggatcacc tggtgacgta gtatctgcat
     3841 acgcactggt tcctaccata cctagcaata cagttgcact aaataaactt cctaaaatca
     3901 tttttttcat gtgtttttcc tcttctctta tttattgatt tatttcaaaa tcatctagtt
     3961 cgggaagcgt ggaaactaga taattttttt cctgttcttc caattgaaaa gacacttttt
     4021 ttgtttcttg tgtctttttc tcggtttcca atgtaatgac atacgcaatt tgccaatgct
     4081 tgccttcttt cttcatttcc caggataaaa tacttttgac tttttccttt tgaggactga
     4141 tttgtttcgc aactttttcc gttagaaacg attgaagctt tttcctatac cgttcttctg
     4201 ccatttcacc cgaataataa ttcgtaataa aataacgact aaatgtatca atttgtggtt
     4261 gttttgtcgc aagttcttcc gcaaatgcaa tgcgttcttg taactggtgc tcctttttcg
     4321 ttagttgttc aatctctctt tgcgttgaaa gttgcataat aaaaagacca caagatacaa
     4381 cgacgataaa tccaaacaaa agccattgaa aagacatttt tttcttctga agagagccgt
     4441 tgctttgtgt aaacgctgtt tctacagaaa gtggtgtttt cttttccttt ttgatttttg
     4501 ttggtcgacg ttcgttcttt tggtctttct ttacttgata aaacttcggt aattcggaat
     4561 ttaaggtttg aataaaggtt tgtgcttctt cacttaattg cccattttct gttaaagatt
     4621 gttcaatcaa tgaaatcaaa tgaaactctt ctactggaaa atcatacgag ccttcaaata
     4681 ctggaatttc atggttttct gtgtagattt ccaaggtaca ccagccaatt tccgctcgtg
     4741 ccaatagctc ttcattttct cttttcaatt gaataaatgc cttatctaat gcaatttttt
     4801 tatctgttag taaagacatg ttatcaactt ctgtaaatcg aatcatcatc attagtcaaa
     4861 cacttcttcc accacttcta ccgcatacac cacttgaata gggttattta aagaaaattt
     4921 cacccgatat tcttgattgt tcaaactgac gatatagcta tcctcatctt cagcataata
     4981 ggtatattcg ttgcctttga cttgaaaata tttttggatc gcttgtcttt gttgccattc
     5041 gccaatcttt ggaaaatagg cgaaatgata gccaaataaa acgcctatcc ctaataaaac
     5101 aattcctgtt actataatca tctggtttcc tcctgtacct gtttatcttc tattgactct
     5161 tttgtcggta agcaataaag aaccagtaac acaagagagc caatcgttgg gacaaaatag
     5221 aaaaaaataa atgcccaatg aaaacctgcg tctcgtaaac gtcgaacgct gagagaaaga
     5281 gacgggacaa gaactacgaa acaatacaca atcaatacgc ctatcaaaca accaattatt
     5341 gtttcagaag cattcaacgc tcctgctagt ccaatcgcta agcctaggcc aaacccaatg
     5401 agggcattca ttaacgcaac gagccaatac tcttttcttg tggctttccc tttgtaatta
     5461 aagctattct gccaaaattt tttataattt gtgatcatat ctttgtcctt tctaatgttt
     5521 atttttgatt ctagtttctt ctgttttact aacaatgaaa cttgccataa aaaaggtggc
     5581 caatacacca ccagctagaa atccacttac aaataacaaa attttttcct cctttctaaa
     5641 aaatttcgcc ataaaaaggc aaagtatgaa cacgataaat accatgtaca tactttgcct
     5701 ttttatgcaa tatatgttaa ctctccaagc aattactgat ttttaaattc tttataaata
     5761 aaataaaaat cgtttgagta aagaaataag tcaatgaaga cccaaac
//
LOCUS       DACDTH010000116         5790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDTH010000116 DACDTH010000000
VERSION     DACDTH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.121"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWK54_002758"
     CDS             complement(99..1229)
                     /locus_tag="IWK54_002758"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9594811.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IWK54_002759"
     CDS             complement(1877..3079)
                     /locus_tag="IWK54_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9594812.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IWK54_002760"
     CDS             complement(3139..3342)
                     /locus_tag="IWK54_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9594813.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IWK54_002761"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IWK54_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IWK54_002762"
     CDS             4879..5691
                     /locus_tag="IWK54_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9594814.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DACDTH010000117         5749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDTH010000117 DACDTH010000000
VERSION     DACDTH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.122"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1128)
                     /locus_tag="IWK54_002763"
     CDS             complement(<1..1128)
                     /locus_tag="IWK54_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9594815.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="IWK54_002764"
     CDS             complement(1164..1874)
                     /locus_tag="IWK54_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9594816.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2757)
                     /locus_tag="IWK54_002765"
     CDS             complement(1942..2757)
                     /locus_tag="IWK54_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP9594817.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="IWK54_002766"
     CDS             complement(2768..3736)
                     /locus_tag="IWK54_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9594818.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>5749
                     /locus_tag="IWK54_002767"
     CDS             4499..>5749
                     /locus_tag="IWK54_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9594819.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT         1772 a         1183 c          915 g         1879 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggagctg aagaaacaac gatttaaatg taagtcttgc caaagaacat tcgtagcgga
     4801 tacctctgtt gctgaaaaac actgtttcat tagtcaaaaa gttcgttggt ctgtcattgc
     4861 tcgcttaaaa gagaatactt ccatgacgga aatcgctcgt cagaaaaata tttccacttc
     4921 ttcggtctat cgtgtcatga agcgattcta ccgcccattg aatcctttca aacagacttt
     4981 gccaaaggtc ttatgctttg atgaattcaa atcggttcga agtgttgcca gtgcaatgag
     5041 ttttatcatg atggacggac aaagccatgc attattggat attgtcgaaa atcgacgctt
     5101 gccttattta gaacgctatt tttctcggtt ttcattagcg actcgtgaag ccgttcaatt
     5161 gattgttatt gatatgtatg cgccttatgt ttcgttggtg aaaaagcttt tcccgaatgc
     5221 tcagttgatc atcgatcgtt tccacatcgt ccaacatatt ggcagaactt tcttgaatca
     5281 tcgagtgaaa gaaaccacgg tacgacttaa aggaaattcc cactctcaac gagggttagg
     5341 gaaaaaattg aagcgctatt ggaaactcct acaaaaagaa gaagcaaagt taaactatga
     5401 aaaacgggtt tggcgtgcca gttttaaaga ttatttgact gaaagtgaga ttgccgatcg
     5461 actgttagct gcttgtccca acttacgcca aggctatcag ttataccaag acattcttta
     5521 tgcggtaaag aaaagagatc agtcactatt tgaggattgt ttaactagag acattagcgg
     5581 tctgccagaa acctatacaa ccactttacg aacgtttaaa aagtttctgc cacagatcca
     5641 aaatgcgctt cactacagct attcaaacgg accattggaa tgcttaaaca accacatcaa
     5701 agtattaaaa cgcaatgcgt acggctttcg caacttttac aatttcaaa
//
LOCUS       DACDTH010000118         5614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDTH010000118 DACDTH010000000
VERSION     DACDTH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.123"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(59..2008)
                     /locus_tag="IWK54_002768"
     CDS             complement(59..2008)
                     /locus_tag="IWK54_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9594820.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="IWK54_002769"
     CDS             complement(2050..3108)
                     /locus_tag="IWK54_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP9594821.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="IWK54_002770"
     CDS             complement(3227..3682)
                     /locus_tag="IWK54_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594822.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="IWK54_002771"
     CDS             complement(4266..5069)
                     /locus_tag="IWK54_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9594823.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2076 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat attt
//
LOCUS       DACDTH010000119         5205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDTH010000119 DACDTH010000000
VERSION     DACDTH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.124"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(4..1029)
                     /locus_tag="IWK54_002772"
     CDS             complement(4..1029)
                     /locus_tag="IWK54_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9594824.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            complement(1274..2545)
                     /locus_tag="IWK54_002773"
     CDS             complement(1274..2545)
                     /locus_tag="IWK54_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP9594825.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(2829..3776)
                     /locus_tag="IWK54_002774"
                     /pseudo
     CDS             complement(2829..3776)
                     /locus_tag="IWK54_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            3788..4261
                     /locus_tag="IWK54_002775"
     CDS             3788..4261
                     /locus_tag="IWK54_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9594826.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(4434..4988)
                     /locus_tag="IWK54_002776"
     CDS             complement(4434..4988)
                     /locus_tag="IWK54_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9594827.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT         1657 a          976 c          762 g         1810 t
ORIGIN      
        1 tttttaatcg atattttttt cactttctct aacaataata ttattagaaa caatcacttt
       61 gatcgaaaca tttctaactt cttttattcg ttcattaacc atgcgaactg ctagttttcc
      121 aatttcttct gtattcacat tcaccgtatt taatgcaggt gttaaaaatt cagcaacttc
      181 aatattatca aaactcacaa tcgaaatatc ttctggaata tgtagaccat ttttttgaag
      241 tgaacgataa acccccacag caataggatc atttcctacc atgatagcag tcggcaactt
      301 atctttagca tgttttttca aaagtaaatc tgctgcctgc attccgtcaa gtgtggtcca
      361 gccttctaaa taaatttgac tatatttttc taatcctttt tgtttcatcc acgtctcata
      421 ggctattttt cgataatcat catctgacac gtgtttattt ccttggctat ctaaacgaac
      481 acgtcctcct ccgataaacg caatgtttcg gtgtcctttt ttatacaatc gatctaaatg
      541 ctgattcgtt gcttttgtta aatcagtata aactgcatct acttcagctt caatactagg
      601 gtcatcaatc actacgacat tcgaattaat tgctgatact tcttcaatca cagaagatag
      661 cacctgccca ataatcaaca caccttgaca ttttagcaaa tcgtcttgct ccaacggtgt
      721 ttctgacaaa cgaataattt tcttcaaatt gactttgctt tgttctgctt cgacttgaat
      781 ccctctgcga atcgttcgaa aatagggatc ttccaactca tctacttcag aaactgtcgt
      841 tattaatgca atatttgtta aattttttgc aacgggactt tttttgacat aatgataaaa
      901 attaaccgat tctgcaatct ttcgtttggt ttcatcggtt actgaaagtg tcggatcctc
      961 atttagcacg cgcgatactg ttgcaatgga cacacccgcc cgttttgcaa catccctaat
     1021 tcctaccata atagctcctc ttttcatatt tgaaagtgtt tactaacata taatcataca
     1081 ttttgaaaat aagcaaattt cgttaaaaat aggcactgta atcataatta aaaacaagaa
     1141 aaaatatccg aacaatttag agtaaataat gctaattcta gtgaatttgc gacgaggaac
     1201 tgctagatca cagttcattc tctctatcgt tcggatattt tattgttaac tatacttatt
     1261 tcacagagct tttttatttg attttgtgaa cagagtctcg agaaatcaat tctccctcta
     1321 atagtagtac ttgatttttt atttcttctt gtttggccag tccaatcagt tggtgaaaag
     1381 caatcgcccc cttgttacct ggatttagtc gtattgttgt caacgcagga tccattaatt
     1441 ctgcaaaagt aatatcatca aatccaataa tcgataattg ttccatcact cgtttattct
     1501 tattttttaa aaattttact aatttagccg ctaaataatc tccagtaaca acaattgcag
     1561 taaattcagc taattcttga taaattcgct caaattcaga gttgttatat atccaatgcg
     1621 atttaaaggt taaatgatat ttctttaatg tatcgatata agcttgatgt cgttcttgaa
     1681 taacacttgg ttcacctaca atgtagtcaa aagaaagaaa cgctattttc tcatgaccat
     1741 aatgaatcaa atgttccact gccaatgtac tagcttggta atcatttgta tttacaaata
     1801 aacgattcat ttgttgttca agttctctta atgtctcttt tgaaagatgc gtatcaatat
     1861 aaacaacagg accatcaaaa acgttattta attccaaaaa ttcttttaca cgtacaccaa
     1921 caacaatgaa cccctcaaaa cgccaatttc tctgaatagt tttaacatct tctgctgaat
     1981 taacattatg caccaataca aaataaccat gattctttgc ctgttcctca attccagaca
     2041 aaacttctgc ggtaaatgga tcagaaaata caaaatcagc attatcaata gccgaaaaat
     2101 aaagcaatcc tattaaaaat gcaatcgtct taccgattcc ctgacagaca gaacacattc
     2161 cactaggatc attaaatgaa taagcatttg catatcctaa actaggtgtt gcaaaacatg
     2221 aaaacaacac acgaaataac gcattgatgt tagtaatcgt ccctaacgtt gagcgcgcac
     2281 tgccaccaat cttcttttga ttgaccacaa tcgccgtaga aagattatca atctcatcaa
     2341 cctctggacg actgtattta ggtaaaaata aacgggtaaa actatcatag gtgtcattca
     2401 gttgacgacc agcttcttgg gtaatagtat caaaaacaat cgatgatttt cctgaaccag
     2461 atacgctggt aaaaatacta attttatttt tgggaatttt gagactgaca tttttaagtt
     2521 gtttctcttg cgtggataat ttcaatatac tcgttcattt ggtactcccc tttattttga
     2581 aatagttgag aatgtttcaa tgagacacgg tatttttggc agatgtttag tttacctaga
     2641 aagaggtata aaaatcaaga aaaaaagaga ggctgactca taagtagttt tattagtcga
     2701 aaatgatagt ttataaaaat aggaaccgca tcaaatcaac attctttaga atggatttga
     2761 tgcgtttttc tttatttttt gacttatgag tcaacctctt actcttcgtc atggatttga
     2821 ttagctaatt attgctccac ttgttcacga atgaaattta agtactgcgg taaaaataaa
     2881 ggaatttctt caaagcgtgg actttgattt tcatacgctt ttgcatctag gtttccttcc
     2941 cctgtagcaa tttcaatcaa taattgattt gggtcacgat aataaagtga tacaaaaaat
     3001 tcacgatctt ttattcctga atttataaaa ttgcgctctt gtaattgttt gtcgatgcgt
     3061 tgtaaatcag atttattttc tacgccaaac gcaacatgat ggacgctacc aatcccttca
     3121 atggcaactg gattctcacg atcttgaata atctgaactt cttgatagaa ctgagtgtct
     3181 tgattttcta agattgtcac tcgttgatga cccgaaaaga aactgacgta ttctttgtgc
     3241 taccagccta ccgacaataa ctctcgttca gtcgcatcgg aatattgtac gcgtagttga
     3301 atggcatcaa ttcctaaaat ggcatgctct gtagggatct ggtctgtgac atacgggaaa
     3361 taatcatcaa tatcttcaaa ttcgcgtaag ggaactaaag ctaatcgtgt tgcatctggc
     3421 ccttcaaaac gtaagatagg tcgtccagaa aatgattcga ttccataatg gcaaacgcct
     3481 tgtgtttcaa aatatatttc ccaaaaatgc aatgatgctt cggaaggaac ttttagtatc
     3541 gttcgttcaa tcgtattggt tccaaaaaac tggttgttcc cttcttgtac ttcaaaaaaa
     3601 gtcagttcag ttcccacacg gtggtgatta tctgaaaaaa acaggtgata catggtatga
     3661 tcgtcttgat taattgtttt cattagtaat tttaaaccga gtatattatg ataaaagtca
     3721 aatgtcggtc gaataaagcg attcacaata gaaatgtgat gaatacgtgc gggcataata
     3781 tctaaaaatg agaatcaaac ctataaaaga tggtgaacaa cttttgtttt catcagaaga
     3841 aaataagccc ctttatttag actatgtaaa tcacaatttg aaaatcatca ttaaagagaa
     3901 taacttaaaa catatcacac ttcatggttt tcgtcatacc cattgtagtc tgctctttga
     3961 atctggtgcg tctgtcaaag aagtgtatgt tcgacttgga catacagata ttaaaaccac
     4021 tatggatgat atatacaccc atatcgcgaa aaagcagaca gaggaaacgg caattcgttt
     4081 tgcagatttc atgagttcta cagaggaaaa gcaaggaagt ttgcagaagg tcaaaagtat
     4141 ggtcaaaaac gaaaaaagca gactatccct attagatagt ctgctaaacg ttgagttatc
     4201 agtatttttc ttattccgca gcgtttgcgt ttttgagacc gtattgattc tcttttttta
     4261 atttcatcct tttccaacct aaaaatgtta tttaattact tagtttatat aacacgaacc
     4321 tataccaagt accagtaacc ccagctttta ttccgcgaac tttatgaaat aaaatcgttc
     4381 tagataaaaa tagcttttaa tctttgtctt tactgtttat aaaaattttt aaattataca
     4441 cctagtttaa atccacgaag tgatgctcct atttcatctt cagttatacc aatgtacctt
     4501 tttgtaattg cttgcgagct atgattaaaa ataaacatta aggtagcaat gtctctagtt
     4561 ttcttgtagt agtgataccc aaaagttttt cttaatgtat gtgttcctat atcatctcgt
     4621 cctaattttt ctgctatatc tgcatatatc tgatagacac gatcccttgt tatatgtcga
     4681 ccttgttgcc gactcggaaa taaccaatca ttttcttctt taccttcagt atataataaa
     4741 atttcttctc tcaaattatc tataaaaatc ggttttcgct tacctgtttt atgttcaaca
     4801 ataataggct tacttttgcc tcgtatatct ccaactttta atcctataat atctgacata
     4861 cgtaacccat tattgattcc aaacaaaaat aaaaaactat ctctttccgg attacttgtt
     4921 cttcttagcc aaaactgaaa atcttgtatc tcttcatttg tccttaacgg ttgtacatta
     4981 tagctcacta ttgtttccct tcttaaaaat attctgagtt aatgattatg tatatatttt
     5041 accataatca ttaacttata tatataaact tttgaaagtc aataaaatca agggatttag
     5101 tttatgattt tatatcattt cagtaactca gaatattccg acttttttgt acaaaaaaat
     5161 taaccttact tatatgaaga ttctaagctc ttatttaaat tttag
//
LOCUS       DACDTH010000120         4894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDTH010000120 DACDTH010000000
VERSION     DACDTH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.125"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..562)
                     /locus_tag="IWK54_002777"
     CDS             complement(<1..562)
                     /locus_tag="IWK54_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP9594828.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(749..1399)
                     /locus_tag="IWK54_002778"
     CDS             complement(749..1399)
                     /locus_tag="IWK54_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594829.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1942..2877)
                     /gene="whiA"
                     /locus_tag="IWK54_002779"
     CDS             complement(1942..2877)
                     /gene="whiA"
                     /locus_tag="IWK54_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9594830.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2911..3909)
                     /locus_tag="IWK54_002780"
     CDS             complement(2911..3909)
                     /locus_tag="IWK54_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9594831.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3906..4790)
                     /gene="rapZ"
                     /locus_tag="IWK54_002781"
     CDS             complement(3906..4790)
                     /gene="rapZ"
                     /locus_tag="IWK54_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9594832.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
BASE COUNT         1486 a         1052 c          757 g         1599 t
ORIGIN      
        1 ctataaacat caccccaaat aaaacagaaa acagtataag cattataaaa attatcaaca
       61 accttgatcc ttcttttata gattcaggaa acagtataat tttagtacta gataaattgt
      121 ttacaccgat tagtgttaaa atatctttat tcttcttgct aacataaccc tttatgcaca
      181 aaagaacagc tatacttaaa gtaacaaata aataacgtat tccaatagaa aaatcactat
      241 aaatactctc tagcatagga ccgacaattg aagaagtcac tactgcaaat gctggaccta
      301 tccaagcaac attagaatgt tctttctctc ttttcctttg taaatctttg ttgagtatta
      361 atctatttat ttcttctgca ggcaagttaa ttttcataac cttatggggg attatccaat
      421 taaaaatcgg aaaaaggtag cctccccaaa aagtatagtg atctgtgtca atcagataat
      481 aacctgattt tgattccaga ataacatatc tcaaagccga acgaaaattt ttcatgaata
      541 tccataatct cactgtttcc acacctaatt cctccaattc caattaaatg gatttatagc
      601 atcaccaatt atgttaactg cctgaccaac ctcattgata aaattggcac cactatccca
      661 tgcttcaaca atgctatata tctatcaaac aaaaaatgga aaactaaata ctcgcgcaca
      721 taaattttac attctgtttt ttataagact aataataatc attgaaaaaa tttcttatcg
      781 gtggcgcata ttcccaattg attaatgtga tcccatattc tgcaagtatt ttacgagctt
      841 cttctaagtc atagtgagaa aaatgcataa aacctgcaat acgatcacgg actgtgagct
      901 gtggcttgct tccaccatca ttcataaaat atccagcaga gccactactt aaagtgattg
      961 ttacatcttc actttctatt attaagaatt cataatgaaa tgctttcaac tctccactat
     1021 tatttaaaag ttttatactc caacttcgtc taggttgagt tgtcacattc cctctaaaac
     1081 ttcctataaa ttctgcaatc accgtatctt caatcaaaat tctttcctca tctatttcat
     1141 attcaagact aaacggaaat tctccatatg taatctctgg caatggtggt gctgtagttc
     1201 tccaaataaa tacaataact gtgaatagaa aaaatccaac gatcgatata aatgttatga
     1261 gacctgctcg aagtaaaacc cgatataata aaaatgctgt atttcgcttt gctatatcta
     1321 gaaaaataaa aagtactgca atcataatga tagtaaaagt tgtaaaaaag cacatttttt
     1381 gccctccctt tataaacatc gattctcaaa ttctaaacca aatcttgata gttcaagttc
     1441 aggtataacg tataccaacc atacatccca attactattt atgaacttat gattatcact
     1501 gaataaatca ctccatcccc ttgttaaaac ttctacatta attacacctg gttgttttcg
     1561 actagctaat ccaatgaact tatcaaccat ccttatccac caaccacgag aatcttgacc
     1621 agaagtaaga aaaacagttc tcaaggttga tcgagcagtt ccttccagtt tttaattaat
     1681 ctccagttat agataaatac ttgatcctat ctatatcagg acttctatat taagcaaaag
     1741 tttatgtata aaactaaaat cttcattcag ttgtctcccc gctttttata cgattgtatt
     1801 aaaaaacaat agaagccttt caacattatg ttactccagt aaagttggaa ttatagtttg
     1861 acggaagaaa agcaagtata gcttttaaaa aaaactgata cattcgcatc tcctgcttca
     1921 tgtatcagtt ttttctttgg attaaactgc ttcttcacgc agtttttccg caaattcatt
     1981 tattttgcga attcggtgat taattcccga ttttgatatt gcacctgagg gaatcatttc
     2041 ccctaattct ttcaagctga cctctggatg ttgaagccga agttctgcga tttcctgaag
     2101 tttttcagga agggcatgta gaccgacctt tgcttcgatc aattcgatgt tttcgatttg
     2161 tttagaagcc gcatcaatcg tcttgttcag atttgctgtc tcacaattga ctagacgatt
     2221 gaccgaattt ctcatatccc gtacgattcg aacatcttca aatcggagca ttgagttggt
     2281 tgcaccaatc aatgttaaga aatcagcaat tttttctgct ccttttaaat acgaaatata
     2341 gccattgcgc cgttcaagcg ttcgagcatg tagaccgtaa taatttaaca tatcgcaaat
     2401 gtcttggtta tgctgttcat aaatagaaaa tatttccaaa tgataacgac tcgtttccgg
     2461 attattgact gaacctgagg ccataaaagc accacgtaag taagaacgca ttttttgcga
     2521 atttcccata atctcgtccg aaacgtgtcc attgaacatc ataccatcca tgatatcaag
     2581 atccatcaac acatcttttg tgccttgctt caaccggacg atatagacat tgttcttttt
     2641 taatttcatt tttttacgaa cgagtagctc acttcgtacc tgataatgat ctttcaaaag
     2701 agaatagata cgtctggcaa ttgcagcatt ttctgtttgg acgttcaaaa taaactgctg
     2761 gttcacaata cctaaagagc cgttcattcg cagcaaagca gctaattccg ctttcgcatg
     2821 ttctcgatga acttccaaac cagttagttc tttcttcaca tctgcagcaa atgacatcct
     2881 attctcctcc tttctccaac aataaattgc ttaatatttt gaaccgaaaa ctaaacggaa
     2941 caattcttct actactttgt ctccatcgtg gaagacacca ccatctttca attctaaaaa
     3001 gtcggtggaa atcactcgac atccttcatt tctcaatccg ttgaagtcgt gtgcgacttg
     3061 tactagatac tcatcataga tctctgggtc catataatct tcaggcactt tttccgtatt
     3121 gaccaatact gtatcgataa aaggctgatt caaatgcttg tgtaaaacac gtatatgatc
     3181 ggcatctgtg aaatgttccg tttccccttt ttgggtcatg atattacaga tatagacggt
     3241 ttcagcagtt gtcttgagca ttgcttcgcc gatttctggt atcactaaat ttggaaggat
     3301 actggtgaat aaacttccgg gtcccaaaac gaccatgtcg gcgtccataa ttgctgaaac
     3361 gacttttcga gccgctttgg cttcactttt atcatttgtg ttgcagacat acacacggtc
     3421 aatggtttta cggttgatcg cgattttaga ttctcctact gcagtcgttc cgtctttaaa
     3481 aacagcatgc aaggtcaaag gagtttctga agaaggataa acatgtccat ctacatgcat
     3541 catttttgct aataattgaa tcgcctcata agtgcttccc cgcatctcgg aaactgcggc
     3601 aatgatcaga ttaccgatcg tatgattggc taaaaactga tcttcctttt tgaaacggta
     3661 ttggaaaata tcttcataga attgtggcat atccgataac gccactaaga catttctcaa
     3721 gtcaccagga ggagccatag cgatcgattc tctgattgca ccgctgcttc caccatcatc
     3781 tgcaacagtc actactgctg tgatatctgc tccttgattt cgtaaacttt tcaggataac
     3841 tggcagacca gtgccaccgc cgaccactac gatctttggt tttcttatcc ggtaggtttt
     3901 cattttcacg accggttcac cgtctctttc cttttatcct tgtcccgatg tgtcacattg
     3961 actttatagt ttttccctaa agcatttgcc aaacgttcag tcaatgccac agaacggtgt
     4021 tgaccgcctg tacagccgat tgcaactgtc aagctgcttt ttccttcttt aatgtatcca
     4081 ggcaatacag tttccagaag atccaaaaat ttatggtaga atgattgcgt ttcatcaaag
     4141 gacatcacat aatcatagac tggctgatct tttccggtca atggtcttag ttccgggata
     4201 taatgagggt tgggcaaaaa acgcacatcc atcacaatat ccgaatcgat cggcaacccg
     4261 tatttaaaac caaatgatac aaattcgatc cgaaatcctt ggtctccctt tgatttgaat
     4321 gactcattga ttttttcacg taattgtcgt ggtgtcaatg ttgtcgtatc aatgatcacg
     4381 gaagcttggg ctttcaagtc gtctagtatc gcccgttctt tacgtatccc ttcagtgatc
     4441 aaaccgtcca tcgccattgg atgtgctcgt cttgtttctt tgtagcgagc cactagttct
     4501 tcgtctgaag catctaaaaa taagatagaa gtgtcgatga agttcgtatt ttcgatttcg
     4561 atcagcatgt tttggatttc ttcaaagaag gaacgagaac gcaaatccac tcctaaagcc
     4621 atttttgtga ctttacctga ttctttgatc aattcccaga attttggaat caatcgaggc
     4681 ggcatattat cgatacaaaa atagcccata tcttcaaaac tttggatagc tactgttttc
     4741 cctgcaccac tcatacctgt tatgatgact aattgcaaat tatctgtcat atttacgaac
     4801 ccctctcaca ttgtgcataa taaaattata tcatcccggg cgtatcatta ccagttttct
     4861 ttttctttta caaaaagatt tggaccaaat atct
//
LOCUS       DACDTH010000121         4875 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDTH010000121 DACDTH010000000
VERSION     DACDTH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.126"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            330..1607
                     /locus_tag="IWK54_002782"
     CDS             330..1607
                     /locus_tag="IWK54_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9594833.1"
                     /translation="MEFWNSKGSIGTIGTTDSVGNPFPGASTPTPIEDNSLVIRTNGD
                     GKYILISPKVGKGLVLLGNGKAIYFGDLDVQGKLTVKGKEIIPGQGGGPSGGGESTGG
                     YPSELKTDAEKRAWRIYDILCNNGFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYG
                     LVQWDGSSYPLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGV
                     VQPTTVDGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGE
                     AGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGI
                     AYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQ
                     TYEQNTELGMICGKLDRQYYSASAISSIVIPPK"
     gene            1620..2528
                     /locus_tag="IWK54_002783"
     CDS             1620..2528
                     /locus_tag="IWK54_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP9594834.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            2528..3148
                     /locus_tag="IWK54_002784"
     CDS             2528..3148
                     /locus_tag="IWK54_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594835.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            3152..3601
                     /locus_tag="IWK54_002785"
     CDS             3152..3601
                     /locus_tag="IWK54_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594836.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKAKLDGLANITFEKVGTV"
     gene            3601..4095
                     /locus_tag="IWK54_002786"
     CDS             3601..4095
                     /locus_tag="IWK54_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594837.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            4109..4540
                     /locus_tag="IWK54_002787"
     CDS             4109..4540
                     /locus_tag="IWK54_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594838.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            4542..4679
                     /locus_tag="IWK54_002788"
     CDS             4542..4679
                     /locus_tag="IWK54_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9594839.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
BASE COUNT         1710 a          713 c         1096 g         1356 t
ORIGIN      
        1 gcagtgcagg aggatatgta gttttatggc caaaagaaaa accttccaac atctttatta
       61 tggataatcc agatttagaa aaggcaagag aagtactaag aatgaataaa aatgggattg
      121 ctttttctaa gaaaggatgg cgggggcctt ttaattcagc ttggacatta gatagtatat
      181 ttaatgccaa ttttatacaa actggattaa tcaaagcaga tatctttcaa aattctttta
      241 acaagactgg agatgtattg aaattagtta atggactact ccaaatttgg aacaacaaaa
      301 agaaaataat ggagttaact aaaaaaggaa tggaattttg gaattctaag ggttcaattg
      361 gaacgattgg aacaactgat tctgttggta atccttttcc tggggcttct actccaaccc
      421 ctattgaaga taattcttta gttattcgta caaatggaga cggcaaatat attttgattt
      481 ctcctaaagt aggaaaagga ttagttttat taggaaatgg taaagcaatt tattttggag
      541 acttagatgt acaaggaaaa cttacagtta aaggaaaaga aataattcct ggacaaggtg
      601 gtggtccatc gggaggtgga gaatctactg gtgggtatcc tagtgaatta aaaacagatg
      661 cagaaaagag agcttggaga atttacgata ttttgtgtaa taacggattt acgaaacaat
      721 ctgcatgtgg aatattgggg aacattcaac aagaaacagg aggaactttt gatcctgata
      781 ctgttcaaat aggtggacca gcatacggat tagttcagtg ggatggttct tcatatcctc
      841 ttgttggtcc agcaacatgg gatggaaaag tttatgttca aaacttattt aatgctgcag
      901 gtattaaaga accaataacg agcttagatg cacaagttcg attgcttatt tggacattta
      961 caaatggaca atggatgggc gtagtacaac ctacgacggt tgatggattt aaggcctgta
     1021 ctgatccaag acaagcagca tatgcttttg aacgaaacta tgaacgtccg gcagcgacac
     1081 atcctgaacg tcaagattat gcagttaact ggtataacaa atttaaagat ttaaaaccag
     1141 gaggagctac tggagaagca ggactaaaac atttagagtc tttgattgga caacgaattg
     1201 gcaatggtca gtgttatggc ttgtctgcag aatattcagg atatctgggt ggttgtggca
     1261 tgggtgctgg aacaaaatat ggtttaactc atgtgatagg aaatacttct gcagcatctg
     1321 atattggtat tgcctatgat tggtctgctg ttggttggaa agtgattcaa aaccctagat
     1381 atgatcaatt agtagttggt gcaattatta attgggcaag aggtggacaa gtgggttcat
     1441 ggtttgcaga tggaacttat ggacatactg gcgttatccg aggcttagct aatggtcgta
     1501 tgcaaactta tgaacaaaac acagagttag gtatgatttg tggaaagtta gatcgccagt
     1561 attatagtgc aagtgcaatt tcttccattg tcataccacc aaaataggga ggtgatttaa
     1621 tggcaaagtg gaatgtcata ttaagtacaa cagaacccta taattacgtg gggatgattc
     1681 aagttcgaca aggcaataag aatacagagg ttatggaagc gactatagtt gaaaatggtc
     1741 ttccctacga tttatcagaa tgtaaggtat attttgaatc agttgtaggt gggaaatatc
     1801 cagtccaatt agaaacaaaa attgtggatg ctaaaaaagg gaaaattaac tatatttttg
     1861 ataaatattc catgcagtgt ttacatcgac aaacagccaa tttcattata tttaaaggag
     1921 aagacttgat tggaacaact caagacttct cttattttgt cattaatgct gtttcaaaaa
     1981 cagaaggaga aatgggttct tattggcaat caatcgaaga tttaattgcg gatatgacgg
     2041 actttattaa tgaaaataaa ggcgatttta cggactggat gaatgaaagg aaagaagaat
     2101 ttgaacgttg gagacaagaa caggaacaca gtttccaaga ttggagagaa ggacaagaat
     2161 cagactattt ggcatggttt gaatcaatca aggatatttt aaagacgatt gatccaggtg
     2221 gagtcatgtt agccgaacta acggatgcgc gagtagatat tcaaggagtt cgtcatgcgt
     2281 ctatttcaga gcgtttgctg gcagatatgg actatttata tcagaaattg cgagcaacgc
     2341 ttttcacgat tgaatacggg gagattgaag tgactgatat tttgcaggat gatctctttt
     2401 cagataatca tgaagtcgaa aaagttggaa ctgtagcatt cccgattgaa gaaggagcct
     2461 tgatcatcgc aaccgttgat gatccaaagc aaaatgtgtt cactcttgag aaagttgggg
     2521 tgatctaatg gctaaaacta aacgaatgat ggaaacggat gaaaaaacgg gtgtacaacg
     2581 ccaattcttt ccaatcacac atgcttccgc agttcttggg ttagaagaaa taatagcagg
     2641 agaagcaaca gttttatctg ttaatggaaa aattggagcc gtcgtcatta ctaaagaaga
     2701 tttgggatta gagaatgttc tcacagaatt accctatgca agtgaagaag atgacggcat
     2761 tatcacggca gaaatgtatc aaaaaatttt aaacagtgga gaaggtgact acgtgttgcc
     2821 agttgccact atcgaccgtt tgggtggcat aaaagttggt gaattattga cgattgatga
     2881 gacagggaaa gtctctgcag tcagacaatc tgatgtcaat ttttcgctgg agttaaaaga
     2941 aaaactcgat tcactgaaaa attatactgc tggagaaaac atcactattg atgaagatgg
     3001 aacaattagt tctacaggtg gtagtgggac tggtggtgtc aatcagtcat atgtagatca
     3061 aaaattccaa gaagctgtaa atcaagcaga aaattacaca aacgaaagaa ttccaaattt
     3121 tacttttgaa aaaattgggg aggtatagag aatgacagat atcgtaaaaa taaaacaaag
     3181 caatgtacag gtttatccac aaactcattg gaatgctata gaaggcaaac caacaacggt
     3241 gaagggagat aaaggcgatc ctggccaagc agcaacaatt accataggaa ccgtttctag
     3301 tggttcaacc gcttctgtta cgaatgtagg aacatcatct gcagctagat ttaattttgt
     3361 tttaccaaag ggagataaag gtgatcctgg aataaatgcc acaactacag ctgtagctac
     3421 aactacagca aatgggttga tgtcgtccac agataaaact aagttggatg gaattgcagc
     3481 tggagcacag aaaaatccag gtaatgctac aactacgaca gcaggtttaa tgtcagcaac
     3541 tgacaaagct aaactcgatg gattagccaa tattacattt gagaaagtgg ggacggttta
     3601 atggctgata ttgtgcaatt aaaagaagac ggagttgcta aatacttaaa aacgcatgta
     3661 gaagctattg atggtaaaga agcattggtg cagacagatg gagaccaagc cattgcagga
     3721 cataagaatt tttcaggttc tgtaacaatc aataataaac gtgttttgac gactgatgat
     3781 aacagatatg aagtggtaaa tctggttgtc actaacggta atacagggac agcaaagctt
     3841 tatcgtgaag gaaaaacagt ttctatatat tttgtggctt taaacggaaa aagtagtggc
     3901 gggaatgatt cggttatttt aactgttcca gaaggctatc ggacaccaat tagttttgaa
     3961 caactggttg ggtcaataga ccgttctact ttgaacagtg ctcaactatc tattggcgca
     4021 gacggagcca ttaaatggag aagaaattca agttatggat cagcttattc atttgttatc
     4081 acttattcaa tttaagggag gaaatctaat gaaagtagtt tacaaatcaa tcaagcctta
     4141 cggattcgag caaatcattt tgaacaatca agaaaatatc cctgaaaact gtacagagat
     4201 taaaccacca gttcctaact ggagaccaag atttgatttt gataaaaaac agtgggttga
     4261 gttagctact gaagaagaga aaaaagggaa tgctgttgat agcgtagatg aattggctaa
     4321 tattaaggca ttatacgaaa cgctgaaggc agaaaatgat gaattgaaac agctaaattc
     4381 taaagcaatg ctaaacaatg tagcaatcaa gcaagaaaat gtcttattga aagaaaagtc
     4441 agaaagttta gcgcagttga attcaaaaac gatgcttgct tctgtacaaa ataccaagga
     4501 aatcgaagaa atcaaaaaac aattacaagg tgggaagtaa catgtattca tatgaagata
     4561 tcaaactaat gtatgactgg ggctgtttca ctaacgaaca agtaatggtt ttcgttccgt
     4621 tgtgcattac tgaagaagaa gcagataaaa tcattagcaa agaagagagc gcatcttaat
     4681 tgatgtgctt tttattttga ttcaaggagt tgtcacatga ttaatttagg ggaatgggga
     4741 gcgatagcag gatcaataac cgctatcgtt tctttgattt tattagtaat aaaaccaatt
     4801 actgcatctt tctcgaagat tactgagact ctttcaaaag taagtcacaa tttagatttg
     4861 ctgactaaag attta
//
LOCUS       DACDTH010000122         4805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDTH010000122 DACDTH010000000
VERSION     DACDTH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.127"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(207..1169)
                     /locus_tag="IWK54_002789"
     CDS             complement(207..1169)
                     /locus_tag="IWK54_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9594840.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IWK54_002790"
     CDS             complement(1171..2610)
                     /locus_tag="IWK54_002790"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9594841.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IWK54_002791"
     CDS             2795..4741
                     /locus_tag="IWK54_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP9594842.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1535 a          822 c          789 g         1659 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaat
//
LOCUS       DACDTH010000123         4788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDTH010000123 DACDTH010000000
VERSION     DACDTH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.128"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            564..989
                     /locus_tag="IWK54_002792"
     CDS             564..989
                     /locus_tag="IWK54_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594843.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="IWK54_002793"
     CDS             2141..3118
                     /locus_tag="IWK54_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9594844.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="IWK54_002794"
     CDS             3128..3625
                     /locus_tag="IWK54_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9594845.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="IWK54_002795"
     CDS             4001..4282
                     /locus_tag="IWK54_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594846.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="IWK54_002796"
     CDS             4284..4700
                     /locus_tag="IWK54_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594847.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DACDTH010000124         4746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDTH010000124 DACDTH010000000
VERSION     DACDTH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.129"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1482
                     /locus_tag="IWK54_002797"
     CDS             <1..1482
                     /locus_tag="IWK54_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594848.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLL
                     DAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKGKKYTITLEGTKPATQV
                     FRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTSPQVQICQVPSTSLGNC
                     TIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEI
                     TGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDT
                     ATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDSTILTIPEGYRPPISFEQ
                     LVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            1645..2091
                     /locus_tag="IWK54_002798"
     CDS             1645..2091
                     /locus_tag="IWK54_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594849.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            2093..2230
                     /locus_tag="IWK54_002799"
     CDS             2093..2230
                     /locus_tag="IWK54_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9594850.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            2268..2561
                     /locus_tag="IWK54_002800"
     CDS             2268..2561
                     /locus_tag="IWK54_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594851.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2558..2782
                     /locus_tag="IWK54_002801"
     CDS             2558..2782
                     /locus_tag="IWK54_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9594852.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2779..3804
                     /locus_tag="IWK54_002802"
     CDS             2779..3804
                     /locus_tag="IWK54_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9594853.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            3994..4067
                     /locus_tag="IWK54_002803"
     tRNA            3994..4067
                     /locus_tag="IWK54_002803"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4028..4030,aa:Ile,seq:cat)
BASE COUNT         1656 a          806 c          953 g         1331 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca acagccacac cgtaccagcc taatctactt
      481 gatgcaccgt attatttgag taaggtggct ttgggtgaga atctaattaa accagaatca
      541 caacaaccag ttactaatag taactatctt attaacacct ataacattaa accaatggta
      601 aaaggtaaga agtataccat cacacttgaa ggaactaagc cagcaacaca ggtttttaga
      661 ccatttttca cacgagcaac aggagatgca tgggaggttg gtgacttaca accagtagaa
      721 gggttaactg atgtttggtc tggaacattt acagcagcag ctgacccaca ccctaccagc
      781 ccacaagttc agatttgtca ggtaccaagc acaagtttag gaaactgtac aattaaatgg
      841 ttaaaactag aggaaggcga cacacgaacc ccgaatattg agcaatataa ataccgagga
      901 atcggcatgc gagactcaaa caatccaaaa gattacgtct gggatctagc accagaatat
      961 gtcgaagaca atctggccac agatattaaa atttctgaaa ttactggtaa agcaaacaat
     1021 tataccgatg ggaaagtatc ggagattaat tcgcagttga ctgcttcaat taatgaagta
     1081 gacaccacag ctaaggatgc tcaaacaaaa gcgaatgcta atgcgactgc tatagatgaa
     1141 ttagacaata agatcgatga acgcattaat gatacagcta ctaccacatt aacagttaca
     1201 aacgggaata ccggatcagc aaagctttat cgtgaaggaa aaacagtttc tatatatttt
     1261 gtggctttaa acggaagaag cagtggtgga aatgattcaa cgatactaac aattccagaa
     1321 ggctatcggc caccaattag ttttgagcaa ctggttggct cgatagaccg ttctactttg
     1381 aacagtgctc agttatctat tggtgcagat ggagccatta aatggcgaag aaactcaagt
     1441 tatggatcgg attatacctt tgcaattact tacacgattt agaaaagcgt gaatcgatat
     1501 gaaggcagca tatagaccaa ttgaacctta cggattcgag caaatcattg tgaatgatga
     1561 agaacattta ccggaagaat gcacagaagt cgaaccaccg attccaaatt ggaaaccgaa
     1621 attcaattac tgggagggaa ataaatgaaa aacatttgga aatatggacg tactggcgga
     1681 gagtacgcag gaaaagtatt ggacgacatg cttgtatccg ttccttacac agatcagcca
     1741 ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa tcgctgatca aatgtttgat
     1801 cctaaattga atcaatggat tattttagcg aacgcactag atcacaacga tttaaacaat
     1861 ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg atttaaaaca gatcaatgcc
     1921 aaactcatgc taagcgatgt agcaattaaa caggaaaata ctgcattgaa agaaaaagcg
     1981 gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt cgttacaaaa tagcaaagac
     2041 atttcagaaa ttaaagagca actaaatcca gcttcaaagg gaggtgagta gtatgtttag
     2101 ttttagcgat gtgaaaatga tgtatgattg gggctgtttc actaacgaac aagtaatggt
     2161 tttcgttccg ttgtgcatta ctgaagaaaa agcagataaa atcattagca aagaagagag
     2221 cgcatcttaa ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag
     2281 gggaatgggg agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa
     2341 taaaaccaat tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca
     2401 atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg
     2461 aagaactaaa gaaacacgat gaaagattag atacacatgc agaaaaattg gtagaacaca
     2521 cgcaacaaat taaaacttta tttagggaga gaagaaaatg aataataaaa cgttcgaagt
     2581 actaaaatgg ttcgcactgg taattattcc cgcactagct actttcgtgg ggttagttgg
     2641 taaagcgctc aattggcagt acacagatat ctgtgttgtc atcattactg gttttggcgc
     2701 gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct cggctgaaag
     2761 cgaagaagga ggaaacaaat gaaaaagaaa attactatta ctgcgatgag cctgttaacg
     2821 gctctttttt tattacccat taatacgttt gcttatacta ttaatgacga gtataattta
     2881 gcgccgaatc aaggagactc cagattagca attcctaaca aaattatttt gcacgaaact
     2941 ggaatagatg caccagcaag aaacgtagcc gccaacatga aaaataatta taacggaagc
     3001 aattcttata ctacagatgt tattggtgac ggtgggattg tttaccgtgt gggtgagcaa
     3061 ggatatgttt cgtggggagc tggtaacgct aatccttatg cgcctgtaca gattgaatta
     3121 cagcgcacat atgataaagc attgtttgaa aaaaactatc gagcttatat tgaatataca
     3181 agagatagtg caaaaaaata tggaattcca ttgactcttg atcaaggaac ttctttattt
     3241 acaaaaggaa tcatttctca tttgtgggtg acaaattatg tttgggggaa ccacacagat
     3301 ccatatggtt acttatcgca aatgggagtt agcaaagaaa agcttgctta tgatttagct
     3361 catggattta ccgatgaaaa tccaacaact tctgaaaaca agcctgtcat tgatccaaca
     3421 cgagctggtg cagctaatcc tacgctgaca gatggaacaa atcacgccca cattgatcag
     3481 tttggggaaa tcgaaaacgc aaacttgcat gtggctggat ggcacattgc taactataaa
     3541 tacgagtata ttttcattat ggactacaat actgggaaag aattagctcg agtaagagct
     3601 gatggaattt atagaccaga tgtaaaccaa gcttataata cttctggaaa tgttggttat
     3661 catgtatctt tcaatatgcg taattttcct agtaagaaag tctatgtaat gatgcgggca
     3721 acgaatgatc cagagggaaa cactaaaggc ggtgcgcaag atttccatga caaacgttgg
     3781 tatttaaata ttcctaaacg ataaaaatag ctcctcgttg aggagcagta cataactata
     3841 ttgacaacta taaaaattat tcgataaaat agtgatgtta tcgcatatct tcactatcac
     3901 ccataaatag tcacactcca agctatgcga taacaggttt gttgccacac attctactgg
     3961 ttgattgttt atggctttat gtggcaacaa ccagtaccct tagctcagtt ggtcagagca
     4021 gacggctcat aaccgtccgg tcgtaggttc gagtcctaca gggtacatta acgtagccat
     4081 ttgaatcgtt ctgtgttaga atttttttga agagtattat acaagctaaa gcttttcttc
     4141 attgccactc aaatgagtgg cttttttatg tatcctttta tggattaatg aaaggatgtt
     4201 tcacatagtt atacttctgt atatttgaaa agttttactt tgatttttaa atagaaagac
     4261 atttgggtta aattgtgaga taataataaa gaagagttta aagcgcaccc caaaccactt
     4321 ccccataagt gtgttacgct ttaaactctt ttatatttga agccattaaa aagcatacca
     4381 tatttttgaa aaaaagtgag aaaaaaggct tacaattgga gtggtagtta attagtgact
     4441 tatttttgat tttatagcac tgatactata aaatatagat atcatcatat tacacaatct
     4501 taatactaac ttaaaaaata tctcctttca caagtatggt gataaaattc gttccgggct
     4561 acctttttag gtagcctact ttaatcttta tacctttctg gatcaacgaa agtatacttt
     4621 atatagtcat aacgccgatg atcgctacgt gcgtccggca cgtcagtcat gaatcggcta
     4681 cactagacta gacagaaaaa ataaggtgtg tagaataata aaaaacacac tggaggattt
     4741 ttcatg
//
LOCUS       DACDTH010000125         4710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDTH010000125 DACDTH010000000
VERSION     DACDTH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.130"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            106..357
                     /locus_tag="IWK54_002804"
                     /pseudo
     CDS             106..357
                     /locus_tag="IWK54_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="IWK54_002805"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="IWK54_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9594854.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1765
                     /locus_tag="IWK54_002806"
                     /pseudo
     CDS             1654..1765
                     /locus_tag="IWK54_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="IWK54_002807"
     CDS             1786..1869
                     /locus_tag="IWK54_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9594855.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWK54_002808"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWK54_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9594856.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="IWK54_002809"
     CDS             complement(2994..3857)
                     /locus_tag="IWK54_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP9594857.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="IWK54_002810"
     CDS             complement(3859..4131)
                     /locus_tag="IWK54_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9594858.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="IWK54_002811"
     CDS             complement(4148..4363)
                     /locus_tag="IWK54_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP9594859.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4710)
                     /locus_tag="IWK54_002812"
     CDS             complement(4455..>4710)
                     /locus_tag="IWK54_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP9594860.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc
//
LOCUS       DACDTH010000126         4633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDTH010000126 DACDTH010000000
VERSION     DACDTH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.131"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            175..519
                     /locus_tag="IWK54_002813"
     CDS             175..519
                     /locus_tag="IWK54_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9594861.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="IWK54_002814"
     CDS             520..939
                     /locus_tag="IWK54_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9594862.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="IWK54_002815"
     CDS             complement(1031..1381)
                     /locus_tag="IWK54_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594863.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2567)
                     /locus_tag="IWK54_002816"
     CDS             complement(1347..2567)
                     /locus_tag="IWK54_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9594864.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="IWK54_002817"
     CDS             2933..3499
                     /locus_tag="IWK54_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9594865.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="IWK54_002818"
     tRNA            3644..3731
                     /locus_tag="IWK54_002818"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..>4633
                     /locus_tag="IWK54_002819"
     CDS             4065..>4633
                     /locus_tag="IWK54_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP9594866.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1683 a          778 c          891 g         1281 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tgg
//
LOCUS       DACDTH010000127         4608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDTH010000127 DACDTH010000000
VERSION     DACDTH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.132"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(84..338)
                     /locus_tag="IWK54_002820"
     CDS             complement(84..338)
                     /locus_tag="IWK54_002820"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9594867.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(449..703)
                     /locus_tag="IWK54_002821"
     CDS             complement(449..703)
                     /locus_tag="IWK54_002821"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9594868.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(896..1171)
                     /locus_tag="IWK54_002822"
     CDS             complement(896..1171)
                     /locus_tag="IWK54_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP9594869.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1143..2096)
                     /locus_tag="IWK54_002823"
     CDS             complement(1143..2096)
                     /locus_tag="IWK54_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9594870.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2708..4201
                     /locus_tag="IWK54_002824"
     CDS             2708..4201
                     /locus_tag="IWK54_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP9594871.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4336..>4608
                     /locus_tag="IWK54_002825"
     CDS             4336..>4608
                     /locus_tag="IWK54_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9594872.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1491 a          774 c          802 g         1541 t
ORIGIN      
        1 gcttgaattc ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta
       61 tgttttcttc tagtctacac accttaattt ttctgtctag ttaattgtag cctatccatg
      121 atcggtttat tagacctttg aggtctatat tttttaacta caatcgacct taaatttaat
      181 tgtttcatta gcttttggac atgtttaaga gatactgaaa tcccttcttt taataaaact
      241 tgatggatct ttgtggcacc atagcgttgt ttattcgtaa aaaaaattgt tgaaatcttc
      301 tttttcaact tattatttct gatttctgta gctgtcaacg gtttattctt ataaaaataa
      361 tagacacttc tagggatttc taataaccga cacaacaaag aaacagtgta atccttacac
      421 catttttcaa taaattgaac aagcgaattt aggttttccg agagaatatg gctgcggctt
      481 tttttaagat ttcaagttcc tcatttaaac gagcattttc ttttctcagt tcagctactt
      541 cttttccagt cagtcctgtg gactgatcag gtaaatataa attcttccat ttgtaaatcg
      601 ttgcttcaga taagccatat tctttcgcta accctcgaac agaacgtcca gtttgattca
      661 gttcaacaat catttgttta aaattttctt catatcgggt cataaaaatt catcctctcg
      721 tagataagtt taatttattc ttgtctctac gaaaagctgt ctaagatttg atactaacac
      781 caaatgttgt atacccccaa acaatacaca tattttgaag gattaggatt aataaagcta
      841 aaaatatagt aagtatacta aatatattta ctatatctag tatacttact atattttaat
      901 cttttgatcg tctattatat ataatccgtt cctcgtcgtt taaagtatca acataggcgt
      961 ccattgcaag tttgactatt tcatacatag gaatatcttt tgcataacta agatcccgaa
     1021 tggtttttag tacgggagga tctacttgaa ctggttttct ttctttcgca gtgtactttc
     1081 gttcccctaa ctcgacatta atatcatctt taattgaaat atcttcagca ccaattttct
     1141 tcttatcttt gttcgttggt aataatgcca tttagtttaa tctcctttcc aagaggtagg
     1201 atatgattgg ccattgagtc agcatattta ggtgtataat caaatccttc aatatcacca
     1261 tgttgttcat agtattcaat tctttcttct aattctgtaa agatgtctgc aaaaatagcc
     1321 caccatcttc gatctacata atcatttaat tggataccat tctcaccata catttctaac
     1381 cgatccgagc ctttaacaat gcttttaaaa atgtttgatt caccatactt atcaattgtt
     1441 ttttgatagt ttgcgtgttg agaggaacgt cgcactgaga aaagaacagg caaaattcca
     1501 atgatttgaa gatgagactc ttcaaaattc gtgacaatcg gaactaaaac tttattaata
     1561 aaatcttctg ttccttccat tgcgtaccgt tttaattctt gcattgcaat aatataatct
     1621 gcagcagtta agaatgcacg aaccacatta tctgttgatg gtgggcagtc gataaagata
     1681 taatcgaaat tttttctcaa ttcttctaaa ggtttaatga aggccaatga tctcttacga
     1741 cgatcaggat atttttcgga caagtattca ttaagttctt cagatccagg agagccagcc
     1801 ataatatata aatgtggact tacttgagta atacctgaaa cccagtcccc ttttttgatt
     1861 gctgcagaca tggatcgatc aaagctagta atttgatacg ttgatccaat gttggaagta
     1921 gtatttcttt gttggtcata atcaattagc aacacgtctt tttcaaaaag ttgactagaa
     1981 acaatcgcta ataaatcatt gtctgtagtc ttaccaatgc cgccttttct acctgtaagc
     2041 attacaactt ttcctagttt atctgatgtt ttaaatgcag tccgtaagtt ttccatagtt
     2101 ccctccaatt tcctatacta aatatattta gtatacttca tatacttaat atattcgata
     2161 tatctaatat actccaaatt gataagaatt cagactttat aaggaatata gtggatatac
     2221 taaatatatt tgctatattc agtatatcag atatattcaa aatgtcaaaa tatatttgtg
     2281 cgttttgcgt tgacaattta gagtgaaaag aggtaaactt aattctatag aaaccacaga
     2341 taattcacta tttacaaagt agcaaaagca gtggtactct ttaaacagaa aaattacata
     2401 aaaaaaagag acggaaataa ttgattttag cagaatcaat tatttctgaa tcacatggtt
     2461 aggacatgtg ttcacgctcg tctcaagaat atgtaagtag ctaccaacta cttatattaa
     2521 aagttgatcg gatacctttt ccgattgcct ttattctacc aaaaaaaggc gagcaactca
     2581 acagaagaac gtttatttag tgtacttaaa ttaaatattc ttaaacactg aagagagaac
     2641 acatactatg gctgagatta aagctaaact gtgttctctt tttgtttgag aaaggacgtt
     2701 acaatcgatg ttgagcgaaa tttattcagc tattcttaaa aacggcttac gtacatacaa
     2761 atatcgaaac agcaccatga aacctgcagg tcatattgat tccagtagga gtggtgctat
     2821 ctttgcgtac cgtagcaagc aattaatgaa tgttgggcgt ggtatggtca ttacttcgga
     2881 agaagctatt ttagagaacg aaaagaaact aacgcattgg acacctaata cctatcgctt
     2941 tggtacttat gcggatgatt atcgaacagt ggttaaaggt cattctgaga aaaacttaag
     3001 tcaaatcaac acatttgtag ttgatatcga ttcaaaagag aaccatcaag gcgaaattat
     3061 tttagcctgt ttagatcaag tgggctacat gccaacgcta attttagaat cagatcatgg
     3121 ttatcaagtt tattttgtac taaagacacc aggctatgtc acaaagaaaa gcaatttcaa
     3181 ggtgatcgat gtagcaaaaa aaatttcaaa aacaattcgt ctacagttgg ctgaaaaatt
     3241 gtccggagtg gatctaggat gtaaccactt cggaattgct cgattcccac aagaaaataa
     3301 tattgttttt aaagaaatga actatcagta ttcttttgct gactggattc aatggtcgat
     3361 gaagcttgaa tcagatcaaa aaacaaaaga agatcgagaa cgaaaactaa ttgtctttcc
     3421 tgagaaaaaa gaaattcgtc aagtagatga accgtggttc gatatgttac ttcataaagc
     3481 aaacatcgtc ggcggagaag gacgtcttgg tcgaaataat gtgattttca cgctttcttt
     3541 agcttattac tcttcaggtt atgcattgga tacttgcgaa tacaatatgt tcacattcaa
     3601 cgaacggtta caggaaccgc tatctgaagc agaattagta aagattgtac gtagtgctta
     3661 ttctggtaat tatcaagcag caagcagaga attcattatt gaattgtgcc agcaatgggt
     3721 ttcaccaacc ttaaaaaata atgatctgtt cattcaacga cgtggctggt ggaaatttaa
     3781 aaagccacga gatcaaagaa aatatagtca taaaaatgaa tggaaagaag atctattggc
     3841 ttatctaagc gaaaaaagtc ttgctagtga gccttatttg aacctgtcaa aaaaagaatt
     3901 ggcttcacaa ctaacgatgc ccaagcgaac acttgataag atcttaaatg agttacgaga
     3961 ggagaataaa gtcttctaca aggtaacacg aggaagaaat ggcggaatga tcattgcttc
     4021 agtcaaaacg ttgttcgcga gggttattca actaagtaaa gaaaagagac tagattattc
     4081 aacaaggatc acagaggtat ttggactcaa cgagagctta gtagaagaga catttagaca
     4141 attatcaacc cccttgaacc ctcttaaaga agtgaatctt tttgaagtag atacaggttg
     4201 aaaaataaaa ggtgcaaatt gccttataca atatatctat gatacttctg taggtgttgc
     4261 tccctttgcc tagctacgtg taggtggtat acttataact gcgtatgcag tcttatgtat
     4321 catggaggaa gttgtatgtc gttaaaaaat tatgtctatt cacatcctgt taatgtgttt
     4381 atcattggtc gtttgggatt atctgtggaa gtgttctgtg agctttatgg ttttaagcag
     4441 gggactttgt cttcttgggt taccagggaa aaaacagttg cttctttgcc gattgagttt
     4501 cttcatgggt tgtccttagc ttctggggag actatggatc aggtatatga ttgtttgtgt
     4561 gtgttagagc aggagtatat tgagtataaa atagctaacg agttaaga
//
LOCUS       DACDTH010000128         4542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDTH010000128 DACDTH010000000
VERSION     DACDTH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.133"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1389
                     /gene="gadC"
                     /locus_tag="IWK54_002826"
     CDS             <1..1389
                     /gene="gadC"
                     /locus_tag="IWK54_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP9594873.1"
                     /translation="GGILWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAI
                     FFQWFQITVGFITMIYFILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTN
                     RTAKIAKAGFVIGIVIPSIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFV
                     SFILAYMGVEASASHINELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLS
                     LSAGVIQTFETLILHFNSHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGL
                     LPVFFRKTNKQDVPVPLIMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGY
                     LLFFLGYFVLVFKKKDLKRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAAS
                     EHRTYQIILLISFVVTVLLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEH
                     HVTKNDEHILKH"
     gene            1402..2802
                     /locus_tag="IWK54_002827"
     CDS             1402..2802
                     /locus_tag="IWK54_002827"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP9594874.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(2967..4407)
                     /locus_tag="IWK54_002828"
                     /pseudo
     CDS             complement(2967..4407)
                     /locus_tag="IWK54_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         1428 a          745 c          861 g         1508 t
ORIGIN      
        1 ggaggtattt tatggtttct accagttgct ttatgtgctg cagaaatggc aacagttgaa
       61 ggatggaaag atggtgggat tttcagttgg gttagtcaaa ctttaggaga acgttttggt
      121 tttgcagcta tttttttcca atggtttcaa attacagtag gttttattac aatgatctac
      181 ttcatactag gagcgctgtc gtatgtaatt aattttccag agcttaatac agatccattt
      241 atcaagttta ttggtgtatt agtcattttc tgggtactga ctttttcaca gttgggcggg
      301 accaatcgta ccgctaaaat tgctaaagca ggatttgtca ttggaattgt tattccttcg
      361 atcattctct ttggtttagc agcagcctac tttatcggcg gtaatccgat tcaaatggct
      421 attaatgcga aagcttttat tcctgacttt tctcagcttt caactttagt agtatttgta
      481 tcttttatat tggcttatat gggcgtagaa gcttcagcat cacatattaa tgagctggaa
      541 aatccccaaa gaaactaccc attggcaatg tttttactag tgattttggc catcgcctta
      601 gatgcaatcg gcggtttctc agtagcagcg gttattccac aacatgactt gtctctcagt
      661 gctggtgtaa tccaaacatt tgaaacgctt attttacatt ttaacagtca tttacattgg
      721 ttggtaaaag tgattgctct catgattgcc tgtggcgtta tgggtgaagt tagttcttgg
      781 gtagttggtc catcacgagg gatgtttgca gctgctcagc gaggtctgtt accagttttc
      841 ttccggaaaa ctaataaaca agacgttcca gttccgttga ttatgataca aggaattatc
      901 gttactattt gggcagcagt cttaactttt ggtggaggag gcaacaattt atcattctta
      961 gtagcaatct ccttaactgt tgtcatttat ctcgtaggat atttactatt tttcctaggt
     1021 tactttgtat tagtttttaa gaaaaaagac ttaaaacgaa cttacaatgt accaggaaaa
     1081 actgttggca aaacgattat cggtggaatt ggttttattt tatcagtgtt cgcattgctt
     1141 atctcttttg taccaccagc ttcaattgca gctagtgagc accgaacata ccaaatcata
     1201 ctacttatta gctttgtagt gactgttctt ttacctttta tcatttatga agtacatgat
     1261 aaaagtgctc acacaactat tgaggaacct actcacgtga aagctgaaga cgttaatccc
     1321 gctgtttatc cagctgctag aggagagcac cacgttacaa aaaatgatga acatatttta
     1381 aaacattagg aggaattcat tatgttatac ggaaaagata atcaagaaga aaaaaactat
     1441 ttggaaccaa tttttggctc tgcaagtgag gatgttgact tgcctaaata taagttaaac
     1501 aaagaatcca ttgaaccacg aattgcttat caattagtac aagacgagat gttggatgaa
     1561 ggaaatgcgc gattaaactt agctactttt tgtcaaacgt atatggaacc tgaagcagtg
     1621 aaattgatga cccaaacgtt agaaaaaaat gcaattgata aatcagaata cccacgaaca
     1681 acggaaattg aaaaccgctg tgtaaatatg attgctgatt tatggcatgc tccaaataat
     1741 gaaaaattca tgggaacttc aacgatcggc tcttcagaag cctgcatgct gggtggtatg
     1801 gccatgaaat ttgcttggcg taaacgtgct gaaaaattag gtcttgatat tcaagcaaaa
     1861 aaacctaacc tggtgatctc ttctggttac caagtttgtt gggaaaaatt ctgtgtatat
     1921 tgggatgtgg aactgagaga agtcccaatg gatgaaaaac atatgtcaat taatctagat
     1981 actgtcatgg attatgtgga tgagtacaca attggtattg taggtattat gggtattact
     2041 tacactggtc gttatgatga tatcaagggt ctgaatgatt tagttgaagc tcacaataaa
     2101 caaactgact ataaagtata cattcatgtt gacgctgcat cgggtggctt ttatgcacca
     2161 tttactgaac ctgatctagt ttgggatttt caattgaaaa atgttatctc aattaattct
     2221 tcaggtcaca aatatggttt ggtatatcca ggtgtgggtt gggtcttatg gcgtgaccaa
     2281 caatacttac cagaagaatt agtatttaaa gttagttact taggtggaga aatgccaact
     2341 atggctatca acttctctca tagtgcagca caactaattg ggcaatacta caactttgta
     2401 cgctatggct ttgatggtta tcgtgatatt caccaaagaa ctcatgatgt tgctgtttat
     2461 ttagccaaag agatcgaaaa aactggtatt tttgaaatca ttaatgatgg atcagaattg
     2521 ccagttgtgt gctataagct gaaagaagat cccaatcgcg aatggacact atatgattta
     2581 tctgatcgtc tgttaatgaa gggatggcaa gtcccagcct acccattacc taaagacttg
     2641 gatcaattaa ttattcaacg cttagttgtt cgagcagact ttggaatgaa catggctggt
     2701 gattatgtac aagatatgaa ccaagcaatt gaagagttga ataaagctca tattgtttat
     2761 cataaaaaac aggatgtgaa aacgtacggc tttactcact aaaattcttc agagaaatat
     2821 aaaagaagtt aattaactaa taaaaggagt tgtgattacc acacagctcc ttaaatctat
     2881 ttgaataaat atatgatatt catttgttac actctcaatc ttagctgttg ttctattcat
     2941 cattactctt attgtaagta gttcagtcaa atttgcttgt tcaattgatc gataacagta
     3001 tgaattgttt gaaaagtaat cggatatata attgttttct cactacctaa gccttcttgg
     3061 tataaagaac tatcgcaaag tataatccgc tgagtacaaa agaaagtgtc gtctactatt
     3121 tctctaagtt gtttacaaat gatattacta atagaaaagt gttctcccaa aatgtcttct
     3181 aactgttgtc gatagtataa atacctaaag tctgacggag ctactatgaa cacttcaatt
     3241 ggagatttcc gtttatgctc atttaaaaga cttaaactaa tggttagttt acgaatagaa
     3301 ttctcattcc aacgcagaaa taaattgttt tgttttcgcc attgttcaag aatttcatga
     3361 acttctaagt acaaagcgcg ttgctcttca tttaacaagt aagtcttatc aggttgaaat
     3421 atctgaatat cagcccaaag tgagcggata aacgggagaa gtgctttatc aaaaagtatc
     3481 tgattttctt cagatatatt cagagataag ataagttttt gactgagctc aatcaccaaa
     3541 gggttatctg ctacatggtt ttgataaaca acgttcaagt ctttttgtaa caattcaaag
     3601 ttatcgcata gataatttct agagttgaat agaaagtaga tataaaggca ttcatcttcg
     3661 tcatgacata aagagaaaga aagttgctct aatcctttct gtattagctg aaacaaggga
     3721 gtatgtcggg caactctttt ttcttcctga ctaaaatata ttgggttagt cttagatctc
     3781 tgcaaaccga tagcgatacc taataaaata aattgttttt caactgtagg gtaacttctt
     3841 gaaaaaaaat gtttttctac ataatcaatc aattgttgac cgctttgaat aattgattta
     3901 tttagctgga tcaactctgt ccgatttgtc tcatagatta tcgttagtaa caaacttcga
     3961 taatcctttt caggaatttc aaaatggcca tcagcattta agtactgcat ttgtttaaaa
     4021 aaattaatca actcatttcg taagttgtaa actttgctta acgaaatgaa ggtagtatca
     4081 gctatattac tccaattgtg ttcccctgat aaataatgtc ctagtacatt gaaaaagtca
     4141 gagcgctgat agatgagttg agtgagtgcc aacctcgaaa acgaagagtc gaatgcgact
     4201 gtaaagctac ttctagattt ctccactgaa ataatttgct gatggagtat gtcggtaatg
     4261 cattccaagt cgttagtgac agacagtgag tgggcaaacg ttcaattgat ctgctatttc
     4321 gcgaatctga atggtccctt tttcgtgtaa ttgctcacaa atgtaaactt gacgaaaaat
     4381 acttttctcc agataggttt ccaacatagt actcctccta gtcattttat acataactat
     4441 tataactcta ccaatcggaa tagtcctgta atatattgaa tcaaaaatat aaattataaa
     4501 ataattagat ggggtatttg gtttaagtat cccttatata gt
//
LOCUS       DACDTH010000129         4475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDTH010000129 DACDTH010000000
VERSION     DACDTH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.134"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            323..1054
                     /locus_tag="IWK54_002829"
     CDS             323..1054
                     /locus_tag="IWK54_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9594875.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="IWK54_002830"
     CDS             1199..3226
                     /locus_tag="IWK54_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9594876.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="IWK54_002831"
     CDS             3334..4431
                     /locus_tag="IWK54_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9594877.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DACDTH010000130         4432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDTH010000130 DACDTH010000000
VERSION     DACDTH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.135"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IWK54_002832"
     CDS             complement(543..2048)
                     /locus_tag="IWK54_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9594878.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IWK54_002833"
     CDS             complement(2045..2731)
                     /locus_tag="IWK54_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9594879.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWK54_002834"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWK54_002834"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9594880.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1545 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat at
//
LOCUS       DACDTH010000131         4419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDTH010000131 DACDTH010000000
VERSION     DACDTH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.136"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(174..1946)
                     /locus_tag="IWK54_002835"
     CDS             complement(174..1946)
                     /locus_tag="IWK54_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9594881.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1900..3654)
                     /locus_tag="IWK54_002836"
     CDS             complement(1900..3654)
                     /locus_tag="IWK54_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9594882.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3764..4333)
                     /locus_tag="IWK54_002837"
     CDS             complement(3764..4333)
                     /locus_tag="IWK54_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP9594883.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1493 a          828 c          610 g         1488 t
ORIGIN      
        1 tttaaaactt tgattattcc ttatattata agaatagaca ttaagtgaac taattcaatc
       61 atactgattc tttcgtgtat tcaaaattag atgctgttta ataaaattat tctttttgaa
      121 aaagaattat aactatatcc caaaaattgt aggttaattt aaagcctatt tcatcatcgt
      181 gattgagctt ctttttcata gacgaatttc tcagatagtt tagttttctt tatgagattt
      241 ttgtatattt ctgaccttcg atacaactca ttatgagtac cttgatcaac tagttttcct
      301 tgatccatca ctacaatttt atttaccttt tcaatagact tcaagcgatg cgatataatt
      361 agtactgtct tatcttgtat taaacgattt aaactttcct gaatacgttt ttcattttcc
      421 gcatctaaac tggcagtaat ttcatctaaa attaaaattg gcgcattttt caaaaaagcc
      481 cgtgctatgg ataggcgttg tcgctctcct ccagaaagtg aacttccgtt ttctccgacc
      541 aatgtatgat aaccgtcagg tagtttttca ataaagtctt cgcagttagc taaacgagct
      601 gcttttttca cttcctcatc tgacgcttga ctatttccaa tacgtatatt ttccaaaata
      661 cttgtattaa acaaagttac ctcttgaaaa acaatagcga ttttgctaaa caaagaagct
      721 ggagatacac gtttaatatc atacccatca attaaaatac agccttcatc ataatcatac
      781 agtctagaca ctaatttcaa gatactagtt ttcccggaac cactagcacc tactaacgca
      841 gtcacttctc cttgtttagc cgtaaaagag atatgatcta aaattggggt attgttatca
      901 tatgagaaag agacatctcg caactcaatg tcaaaagatt tcagaggact atctgatccc
      961 tcttggatac tggtttcctt catagctcta attcgctgta ttttaggagc taaataaaat
     1021 atttcaagca ctgcttcttt catagaatcg aaggaatcct ttatttttat tgcggctagt
     1081 aagtaaccaa cgacgtagag tatagtcact tcacccgtta acagtaagtg aacacctact
     1141 agaatgacta ctgctaaact aacatatgag aaaatagagg ataaggccat gactgaaaaa
     1201 gtacttaatt ctacctttaa atgaatcctt tcactctctt ccattttttt gaaaagtctg
     1261 tcttgaactt ttttagatag attaaagcta ttaatctctt gctgcaattc tatagtttct
     1321 tgaaattctt ccgagttttc tctcaaagta tcgtaatatt tagtattggc tttcgtttgg
     1381 gatttctttg ataacaagat taagacaaaa ctaaataacg ttggcaaaat aacagctaat
     1441 cccattttga cattaccaac caaaagcatc actgaaataa aagggaaaaa cagagccata
     1501 ccgccggctt taggtattgc atgactcatc gcatgctcaa taccttcaac gtcagacatg
     1561 atagtttgag ataaatctga taaattatgt tttgaaaaat aggataatgg taaatttgac
     1621 aaatcgtctg caatttgcac tcttaaatgc gcactttctt tataagtact attatataag
     1681 ttttcatact ccctatctaa caaccaatac atgaaaagca aagtaacaaa cgatacggca
     1741 aaataaagcc aacggggctt tgtattttct aaaactaatt cgtctatcag catcattata
     1801 atgaatgctg gtaccatatt aatgcagtat acaaaaaaac tagaaataga agctttagtt
     1861 aaagcttctg cgccctgatc acttaaagca aatttgtttt tataaaaact tttcattgtc
     1921 aagcctccaa tcatttgcat tctcataatt agtgaccaat tgtttataca cttgatgttt
     1981 agccattaat tcattatgat ttccacgttc tactattttc ccttctgaca agacaagaat
     2041 ttcatctaca gatttaatgc tggataatcg atgagctatc ataataactg ttttttcttt
     2101 catcagatgt tgaaaagcct tttgtagttc atactcatta tctggatcaa tagatgcact
     2161 ggcttcatcc ataataacaa tctttgcatc ttttaaaatt gctctggcaa tagctatacg
     2221 ttgtttttct cctcctgaca gataaactcc tttactacca attaaggtat gctctctttc
     2281 gggaaatttt tctaaaatcg attcgcaacc ggctaatctt agcgcgtcaa atactttact
     2341 tgctggcgct tgaggatttg ctatagaaac gttatcgtaa atactctgat tgaatagttt
     2401 cgtatcttga aagacaaaag caattgtgtt aataattgtg tcttttgaat agtgatttaa
     2461 aggcttacca ccaattttaa tctgtccctt atccacattg tagaaacctg aaattagttt
     2521 agctatcgta ctctttccgc ttcctgattt tcctactaat gcataaattc ttttttgttc
     2581 taaaactaag ttgaaatttt ctaaaacaca ctcttctttg tatgaaaagc tgacattttc
     2641 aaatgcaatg tcacaatttt taatctgtgt ctcgttccca tattttagtt ggtcttgctt
     2701 catttctaaa taaagggagt ctagtgtttc gactgcatat cttgcttcaa acaagtgcat
     2761 acttacatac attattctca tgaaagaaac aaaaataatt ccacttaaaa aaaacaacat
     2821 caacaagtca acagctaata tcttgggagt ggtaagagaa gttataaaaa aggttatagg
     2881 aactatcaaa atgcaaacta tgccaaaaaa tagccattga aaaagaacaa aaggtgtttt
     2941 cccactcaaa gaatacttat atgcaaactt agcataatcg ttaattgctt tattcaaggc
     3001 tttaaatgat gttactttag caccaaatat ctttataact tgaattccgc ggatatattc
     3061 taccgtttca gagcttaaca catctaacga ttcttgatat ttttgaataa aatttgtatt
     3121 ctctgtcatt ttctttaaca agaatacacc aattactaac atggcgatta gaaccaatcc
     3181 aattttaaag ttaatgaaaa agcctaaggc aactgataaa acaggtgtta ctatggcttg
     3241 agcactatct ggaatcaagt gagcaattgc catatgtgtt ttagccgcgt tgtcatcaat
     3301 tgtctttcga atatatcccg aagaatttaa atcaaaaaaa cgaaaacttg cttctgttaa
     3361 accgctaatt cccttttttc tcaaattagt ctctaagcga aacccaagga tatgagaaaa
     3421 taagccagaa aaaatataaa ataaagctcc taaagttaac caagcaacag tttgaaccgc
     3481 ataactctta gctaaatatt catctcctac aataattaga gactgaagaa atttgtaaat
     3541 actgtaatat ccatagactg ttaatacgac agaaaaatca gaaaataaag tggctaacca
     3601 tccgtagtat ttcattttag ggacataact aaataatttt ttgtatactt tcattttttc
     3661 ctcccccgca ttcaatttct tctcttattc gtttacaact gttaatgctt tccgtaatag
     3721 cgtaaattag agatgtacaa acagataaaa ggtaaactac gttctatttt ttgtattttt
     3781 ttaagacttt tcttccaatc aatacaccaa tcatagataa acttgctaca actaacactg
     3841 aaatgattcc ccatatacct accgcatagt ggatcattgc atccattgtt gcagcagatt
     3901 gtcctttatc tatccaatct tgacgaaaac gatctgtaaa gaaccatata ggaatccact
     3961 gtccggcagc actgcctaat tgcattagag cccaagacgc aacttgagga attgctcgat
     4021 catatccaaa tacagataga ataatatcag ctaagatcgc catactaatc gaactgataa
     4081 accaaggtaa gtacgccccg cccattaacg taaaaaataa caataaaaca attgtgtaaa
     4141 tagtaaaaat tccttttcta gggtagttat agctaatata aacaaatatg attccagata
     4201 ataatccaaa aatcccgggg gatatgctgt gaccaaacgc cccaccaata ctactaacca
     4261 ttattgaaaa aaagtaaatt actagggaaa taactgtagc taaaccaata aaaatataat
     4321 cttttgtttt catatatatt ctcctcaatt ctatttcgtt atgtcattaa aaatctgttc
     4381 tatcaaagtt gaatcttcta ctctaagttt tatatcttt
//
LOCUS       DACDTH010000132         4397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDTH010000132 DACDTH010000000
VERSION     DACDTH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.137"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            69..950
                     /locus_tag="IWK54_002838"
     CDS             69..950
                     /locus_tag="IWK54_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9594884.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            963..2573
                     /locus_tag="IWK54_002839"
     CDS             963..2573
                     /locus_tag="IWK54_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9594885.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2650..3309)
                     /locus_tag="IWK54_002840"
     CDS             complement(2650..3309)
                     /locus_tag="IWK54_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9594886.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3592..4257)
                     /locus_tag="IWK54_002841"
     CDS             complement(3592..4257)
                     /locus_tag="IWK54_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594887.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1179 a          857 c          869 g         1492 t
ORIGIN      
        1 taaaaccttt ttattaaact atttaatctt gtttagtata tatttttaga aaaaggagtg
       61 gggtttttat ggcagaaatc gtcaaagtca cacatttgaa aaagagcttt ggtcgtttcg
      121 aagctttaaa agatgtctct ttcaccgtac attcaggtga agtcgttgga tttatcggac
      181 caaatggttc tggaaaatcc acgacgattc ggacactttt agggattata aaaaaagacg
      241 gcggaacggc aacgattttt gggcaagatg tatgggaaga tagtttatct atccatgaac
      301 gtctttctta tgttccaggc gatatctctt tgtggggaaa tttgactgga ggagcgatca
      361 tcgatctctt catgaagctt catggaaaag ggagtgctca aaaaaaagat gaactgattc
      421 aacgtttctc tcttgatcct aagaaaaaag caaaaaacta ttcaaaagga aatcgccaaa
      481 aagtgggctt gattgctgct ttggctgttg attcggatct ctatttattt gatgagccta
      541 cttctggttt agatcctttg atggaaacga ttttccaaga agaagttgaa aaattgaaag
      601 aacagggcaa atccatccta ctttcttctc atattctcag tgaagtcgag cgactagcgg
      661 ataaagtcgt gatcatacgc gaaggaacag tcgtagaaga aggaacactc gatgaattgc
      721 gtcatttgac tcgttcgacc atcactatag aaacagaagc agagaatagt cacttagcac
      781 aattgaacgg tgtccatgac tttgtgcaaa aaggtcacct cgctcaattt gctgctgata
      841 acgaagcttt cgatgtgatt ttagctgaag cgtccaaatt aggtgtgaaa aaatttgaat
      901 ctattccacc gactcttgag gatttattca tgagacatta tgaaggatga ggaggaagcg
      961 aaatgactga taaattttca cgctggtcca cccttttttc tcaaaacctt agccgtgact
     1021 ggaaaaagat cctgatatgg ctagtcggtg tcactgcttt ttctggtggt tttgtccctg
     1081 cgtttgaaga attgacttcc ggacaaggat tagctggatt atacgaaacc ttgcaaaacc
     1141 cggcaatgat tgcaatggtc ggaccaactc cagcttcgaa tgctgctgag tatactttag
     1201 gagcgatgta tgcaaatgaa atgttgcttt tttgcagttt gtttgctgct gtgatctctg
     1261 ttttacacgt ggtgagccaa acgagaaaaa aagaagaact tggtctgatt gaacttattc
     1321 gttcctttcg agtcggacgt caagcaaact ctttagctgt catcgctgaa agtattctga
     1381 tcaatttgct tttagcaata tcgatcagct gtacgatgat ttttttccaa gcagattcga
     1441 tcactgcaga aggtgcttgg ctttttgggc tatctgtagg tagtatgggg attttgggga
     1501 gtgccgttgc actgtttgct gcacaactga tgccaagttc agccagttcg aatggtctgt
     1561 ctttagcgtt tttaggtgga ctttaccttc tacgagcaat aacagatgta gctgattcct
     1621 ccctttcgat gtggaatcct ttaggctggt cttatttgac ctatccattt acaactaaca
     1681 actggattcc tttgctttat ttgtttttgt tcagtctaat cttgataatc agttcatttg
     1741 ttttagagga acatcgagat ttgaacaccg gctatatccc cgaacgtgaa ggaaaaccgt
     1801 atgctaaacg ttctctttta tccttgcgtg gtttattatt ctatctaaat aaagggatga
     1861 caatcagttg gcttatcact tttggtttga tgggggcggc atacggctct atctacggga
     1921 atatgcaaca gtttttagaa agtaacgact taatgaaaca gatgtttatc caatctggcg
     1981 tctcaattga aaaaaacttt acaagcacga tcatggtcgt cttgatttgt ctagctgcta
     2041 ttttaccaat tgctagcatc aatcgtttat ttacagaaga aacccacgga cgtatgaatc
     2101 agctgttcgc aacaaaaatt tctaggaacc aactttattg gaccaatatg ttactagcta
     2161 ttctatttgc tatattggca atcctgatta gttctggtgc actagggctt tctgctttgg
     2221 cttcgatgaa ggatgcgaaa gacatggatc tattgatgtt tcttaaagcc gggttcaatt
     2281 tcctgcctgc cgttttagtg tttatcggaa ttagtggctt tctattcgga tggcttcctc
     2341 gaatgggtaa acttgcttat atctacttag cttactcctt tgcactcaat tactttggcg
     2401 gaattttgga tttaccagaa tggttttcca aaacagctgt ccctagctgg gttccacgga
     2461 tgccaaacga ttcttttgat ctttccgtgt tccttacgtt atcatgtatc agccttgcac
     2521 tgttcgtcct tggttctatt ggttataggc gaagagactt actagaagga taataaaaaa
     2581 gcatgtacaa tttcatgcta gcctccaaat ctgggtcaaa aatccaacat ttggaggtca
     2641 tttttttatt tacaagtctt ctaactgctc tttatcaatg atccagactt ctcgattctt
     2701 tctttttaca taaccattct cctctaataa tttaaatttt ctagataaag tttcgggggt
     2761 cgtaccgata aatgcagcaa gttctttcat tttcattggc aggaaaaagt gatccttccc
     2821 tgtcgcttta gctaagtcaa gcagatagac agcgactcgt tcttctactt tttccatcat
     2881 caaaaaagtt gtctgctgct gtacttttgt taatttctca acgttgattg ctaaaagttt
     2941 cagactaagg gcgggataat gcagtaagag ttccgagaaa tctttttgtc tcaatatgca
     3001 aatttccgtt ttttctagtg cttcgccaaa taaagactca tttggaacgc ctagcaactg
     3061 gctctctccc tcatatccgc ccggctctat tactcgtaaa agctgttcct ttccgctagc
     3121 agataattga tacactttca tactgccgga ggccacgatc actaattgag gttctccatt
     3181 cggcgcaaga atctgttctc ctttttccac atagtgatgt ttgacaagtg cgtgtacttt
     3241 ttcttgatct tgctgtacta agtgattgaa caaaggaaca aatgaaacac ataaatgatc
     3301 atgactcata atctctcctc cttctaaaca gctatttcta ataaagaaac ataacttccc
     3361 ttattccaac tgataatttt cttttatcat agcataaacg tcaagcgaac cagataccag
     3421 caaatagagt ggatttcttt ttgtcccttg ctttattttc cttcttttga gaccttaagt
     3481 aattgattcc tatcaatttt ttaaatattt actttttgaa taatcaaaaa caaaccttca
     3541 ctcgcaagcc gagagtaagg tttgtttttc gcattgctta tttcgtgttg tctattgaaa
     3601 tttttccgca cctagctttt gatatagtgg tgctagttct tgtaaggttt cagcggttat
     3661 ttgctcaacg tgttcgattt cttgcggata gaagaatagt ttacccattt gaaaggttcg
     3721 gtctctcatc gcttttttca actcttcttg tttctttgta tcggacaaat agtcagacaa
     3781 agctaagtgg tcgtcaagtt catgttctgg ttgcggccat atgtaataat ccttgatatc
     3841 gactgttttg ctccactctt tcagtatcgc aagcaactgg ttatacgctg caatgctacc
     3901 atatcgttct tcgcttttat aatgttggta catcaaatag atttggtatt gtttttgatt
     3961 caacaaaact gctagacagg cattctcgtc ttgacgagat tcgcttcggt acgcgctcca
     4021 aaaatgactg cgtaaattcc agccattcgt ccagctttcg attttaggtt tgctcatgcc
     4081 gtacatatca ggcaaaagtg cagctgtctg gagctgaatc tctttccatt tttgccagtg
     4141 ttctttatat tctttcttga tttgttcaat cttatctgtt ggatggctct ctttgagctg
     4201 tttgaatgcg tagattggtc gatcaaacaa ctgataatct gattttccaa gttccattgt
     4261 ttcgcctctt tccgttatct tttgtattct acctgaattt tgcgatatgg acaaatagag
     4321 gttgtgaaaa agctgttctg catcaagtca taagaacagc caaacaaaaa actgcgatga
     4381 gcatcaccgc agtttta
//
LOCUS       DACDTH010000133         4344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDTH010000133 DACDTH010000000
VERSION     DACDTH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.138"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(387..1853)
                     /locus_tag="IWK54_002842"
     CDS             complement(387..1853)
                     /locus_tag="IWK54_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9594888.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IWK54_002843"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IWK54_002843"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9594889.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1308 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaa
//
LOCUS       DACDTH010000134         4310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDTH010000134 DACDTH010000000
VERSION     DACDTH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.139"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(65..1285)
                     /locus_tag="IWK54_002844"
     CDS             complement(65..1285)
                     /locus_tag="IWK54_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9594890.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1910)
                     /locus_tag="IWK54_002845"
     CDS             complement(1305..1910)
                     /locus_tag="IWK54_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9594891.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="IWK54_002846"
     CDS             complement(1959..2891)
                     /locus_tag="IWK54_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9594892.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="IWK54_002847"
     CDS             complement(3312..4172)
                     /locus_tag="IWK54_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9594893.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DACDTH010000135         4210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDTH010000135 DACDTH010000000
VERSION     DACDTH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.140"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            66..767
                     /locus_tag="IWK54_002848"
     CDS             66..767
                     /locus_tag="IWK54_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9594894.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="IWK54_002849"
     CDS             764..1885
                     /locus_tag="IWK54_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9594895.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="IWK54_002850"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="IWK54_002850"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9594896.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="IWK54_002851"
     CDS             3264..4172
                     /locus_tag="IWK54_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9594897.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
BASE COUNT         1446 a          743 c          929 g         1092 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg
//
LOCUS       DACDTH010000136         4153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDTH010000136 DACDTH010000000
VERSION     DACDTH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.141"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..106
                     /locus_tag="IWK54_002852"
     CDS             <1..106
                     /locus_tag="IWK54_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9594898.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            970..1182
                     /locus_tag="IWK54_002853"
     CDS             970..1182
                     /locus_tag="IWK54_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594899.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="IWK54_002854"
     CDS             complement(1460..3259)
                     /locus_tag="IWK54_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9594900.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="IWK54_002855"
     CDS             complement(3383..3979)
                     /locus_tag="IWK54_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9594901.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1228 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt ttt
//
LOCUS       DACDTH010000137         4122 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDTH010000137 DACDTH010000000
VERSION     DACDTH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.142"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..668
                     /locus_tag="IWK54_002856"
     CDS             <1..668
                     /locus_tag="IWK54_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP9594902.1"
                     /translation="FLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKN
                     YSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEI
                     GKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNY
                     ISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFN
                     NIS"
     gene            685..1119
                     /locus_tag="IWK54_002857"
     CDS             685..1119
                     /locus_tag="IWK54_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594903.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1736..>4122
                     /gene="esp"
                     /locus_tag="IWK54_002858"
     CDS             1736..>4122
                     /gene="esp"
                     /locus_tag="IWK54_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP9594904.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT         1509 a          572 c          792 g         1249 t
ORIGIN      
        1 tgtttcttta tgaaaataaa atactggaat atgttaaaaa tggagatatt cgaaatttag
       61 aaaacatggt ttttaatctg agtaatggaa taattccaag tgttagtgga gatacgattc
      121 ggtctgaaaa aaattattct atcattgttt ttgaaaagtt agcacaaacg tctataacat
      181 taggaatgga tataatagaa gcgtatcaaa gtagagatgc tttgatacaa gaaaacgaat
      241 tagctgtatc gttacctgag gtcttaaaag tgagggattc tgggattgtt tattatacta
      301 aggaaatcgg aaaaactaag atagagcatc tttctccctt aatttcttct gtcgttcaat
      361 tcatcggctt aaatatctat aaaagaatta ctgtaaagga aatagctaat tatttttctg
      421 taagtgaaac taaattaaga gaaagcttta agaatgaaat gcatattacg atttataatt
      481 atatctctaa gcgaaaaatt tctacggcta aaataatgtt gaaatcaaac catactatca
      541 gtgaagtttc tttaggttta ggattttcag attcttcaca cttttcgaga gtattcaaaa
      601 agtacgcagg tgtatctcct aagcaatatc aattaggttt agtggacaat ttcaacaaca
      661 ttagctgaga ggttttattt tattatgaga ctatatcatt tacgtagttt ggaggagttg
      721 aggaaattgg attttaacaa aaaatttttt tgggaaattt ctgatttgag ttgtttcgta
      781 gaatctgagc gtctaagtaa tggtaataat agtgtgttaa atgaatatga caaagtagta
      841 attaataaag taaaaagttt atgtgaagaa gctagagaat ctatcagtca tattaatggt
      901 agcaatttta aacaaacatt atttaatctg attaagctag atgctaaaat atcaagttat
      961 ttattctttt tgatacatga tgaatttatg gattatcaaa aattggatca gttaattgaa
     1021 agagaaagct gggaagatta ttacgtgggt ctaacttttt cagaaaagtt aaacaactac
     1081 aagttaatag attataagta tttgagtgaa agcaattaat tattcatgta gagtactaga
     1141 cactttaaat atggacaatt caattataaa gaaaaagaat caataattta atgaggctgt
     1201 gacaaatgtc attttttgtt tcgctaaaaa cgagtgaaaa aatgttgata actccaatca
     1261 ttcgtttaag attctaggaa tcattcagaa atgctgtgaa accgatactt tataattata
     1321 aagaaggtga tttcaaattc cgaagaagga atttgaagtc acctttttac ttatgtcaca
     1381 gtctcattca gtaatgcggg ataaggctcc ttattccgtt atttgaatac tctgaagttt
     1441 aatctaagaa agggtaatat tttgaaaaca tgcttcatcg catagaaatg aacttaattt
     1501 cagaaagaat acatacatat ctttttatat gttttcagct gctgaatggt taaaaagcac
     1561 aaatatccga aaaaaacatg aagaaatgta ctttttttgt tgtaatgttc atgaaattct
     1621 ctaagtaaat tagttaacat aaggttagta agattcctat tctgtgaata atacgtatca
     1681 gatttatctt ttattcttac taggaaagta attattaagg gagttgattt gaataatggt
     1741 tagcaagaat aataagagag tatttctaga aaaaacaaag aaacgagtat tgaagtacag
     1801 tatcaaaaaa ttaagtgttg gggttgcatc tgttttgatt ggtgttggtc ttgtcttggg
     1861 aacaactgaa ctagttaaag cacaagatga aatttctcca agcagcccac ttgaaacagc
     1921 gataagctcc gtacaagtag gtgacaaagt agcttcgggg aatacttttc aggagaatcc
     1981 tggttatacg aaaaattata acttttcgga tttacaattt agtcctcaag aactaactgg
     2041 agacacacta aaaggcaata caattggatt tgaagtttac ggaaaacata atattgcagc
     2101 atcaactaaa aactgggaaa tccgtcttca attagatgaa cgattagcta aatatgtaga
     2161 aaaaattcag gtcgatccaa agaaaggaat tggatcttct agacggacct ttgtaagaat
     2221 taatgattcg ctgggtagac ctacaaatat ttggaaggtc aattatattc gtgcaagtga
     2281 tggattattt gctggggcag aaacaaccga tacgcaaacc gctcctaatg gggtaattac
     2341 gtttgaaaag agcctagatg aaatttttaa agagataggc atagacaatc tcaagaccga
     2401 tcgtttaatg tatcgaatct acttggtaag tcatcaagac gatgataaaa ttgtaccggg
     2461 tattgatagt acgggctact ttttaacaga ttcagatgat ttctataata gcttagatgt
     2521 atctgaaaat aatcctgacc agtttaaaca tggatcagta agtgcaaaat atgagaaacc
     2581 taatactcaa acaaaagatg gttcaggatc cacaggtgct aatggagcta taatcttaga
     2641 tcataagtta acgaaaaatt ataatttctc atattcatct tccgcaaagg gcacgccttg
     2701 gtatgctaat tacaaaattg atgaacgatt agttccatat gtagcgggta tacagatgca
     2761 catggttcag gctgacaaag taacatatga tgtttcgttt gaatccggaa caaaagtagc
     2821 tgatttagca atcgagcgac gggaaggtca tgaaaattat ggcataggtt caataactaa
     2881 taatgattta actaaactta tcgattttgc taatgcaagt ccacgtccag tcgttattcg
     2941 atatgtttta caattgacta agccattaga tgaaatctta gaagatatga aagcaacagc
     3001 acaagttgaa gaaaataaac catttggtga agatttcatc tttgattctt ggttgtcgga
     3061 tacgaataaa aaattaatcc agaacactta tggaacaggt tattattatt tgcaagatat
     3121 tgatggtgat ggaaaccctg acgataaaga agagagcgga gacacgaatc catatatcgg
     3181 gaaacctgaa ttagaagaag tatatgatgt tgacacaaca gttaagggga aagtattcat
     3241 ccacgagtta gcgggaacag gtcacaaagc ccaacttgtt gataaagaag gtactgtatt
     3301 agcagaaaaa actatcgctc caaatgaaaa agatggggct ccaatttcag atactgtaga
     3361 atttgaattt acgggtgtag attcaagtaa attaatcgcg aaagatgaat taaaaatcca
     3421 aatcgtttct ccaggttttg ataaaccaga agaaggttca accgttatta aggaatcacc
     3481 aaaagcggtt gataaacaaa ccgtggtagt tggatttaaa ccagatgcta aagaatcaat
     3541 tcggaataat aaaaacttac ctgaagatgc agagtattca tggaaaacag agcctgatac
     3601 ttctaacgtt actgatagta cgaaaggtat tgtaactgtt aagatcggaa atcgaacttt
     3661 cgacgtggat gtagagtttg ctgtaaaagc ttctcaagct atggaaaatg atgcaacata
     3721 cgtacctata acaacaactc cagaaacgac agttcaaagt ggaaaaccta catttgataa
     3781 accagatgtt cctttagcta aagatgcctt ttcaatttca gatgtctata ataagtactt
     3841 cggtaatgca agtgttgatg caaacactgg tattgttaca ttcactccag ctaaaggtgt
     3901 aggagaatcg gagccgatta ctggaacaat tcctattaaa attgtttacc aagatggttc
     3961 tgtaggcacg accgatttag cagtaactgt aagtaaagat atttatgaaa atccaggaga
     4021 aaacattcct gcaggctacc acaaagtaac cttcaccgca ggggaaggaa caagtattga
     4081 aagtggaaca acagtctttg cagtgaaaga cggcgtaagc tt
//
LOCUS       DACDTH010000138         3987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDTH010000138 DACDTH010000000
VERSION     DACDTH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.143"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..938)
                     /locus_tag="IWK54_002859"
     CDS             complement(<1..938)
                     /locus_tag="IWK54_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP9594905.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKD"
     gene            complement(1160..1522)
                     /locus_tag="IWK54_002860"
     CDS             complement(1160..1522)
                     /locus_tag="IWK54_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594906.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(1526..1834)
                     /locus_tag="IWK54_002861"
     CDS             complement(1526..1834)
                     /locus_tag="IWK54_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594907.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(1843..2262)
                     /locus_tag="IWK54_002862"
     CDS             complement(1843..2262)
                     /locus_tag="IWK54_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594908.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(2255..2488)
                     /locus_tag="IWK54_002863"
     CDS             complement(2255..2488)
                     /locus_tag="IWK54_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594909.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(2491..3459)
                     /locus_tag="IWK54_002864"
     CDS             complement(2491..3459)
                     /locus_tag="IWK54_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9594910.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
BASE COUNT         1131 a          892 c          549 g         1415 t
ORIGIN      
        1 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
       61 tcttcaatct tcgctctctc agaagccatc agagccttat cacgctcgtt ttcttctctt
      121 tgcctataaa tatacctcaa ctcatcagaa acgctctcac gctcttttac agccgtttgg
      181 cgcaattcag aaatttcact catgatttct ttgtattcgt gaatatcttt aattttgttg
      241 gtaccgacaa tatccctttt ccaaccgtaa tcattcatta atcgttctat agagtcaact
      301 tcttgatttc taaaattatt gaaaagcact cgaccattat ctttatcagc gtgtatccct
      361 tgttgaacca gtgctttatt aaagctcggc tgcttttgaa caccccgttt ataaccttca
      421 gcaaccggca caatattcaa gtgcaggtgc ggacttattt cgtcattatg aataacagca
      481 ttataaacag ccaaattagg gtttctctct tgaaactcct tgtaatactg aaccaaaatt
      541 ttattggcaa tcactcgatt ttgttcagct tgttgtttcg atatgccagt agaagaacca
      601 tctgctcgat ttgtctggaa atcatctaaa atgcctattt gcacaataaa ttctcgttga
      661 ggttccaact ttttatcctt gcgcactttc gctaaatagt cctcgatttt tctatcggct
      721 cttttttgtt tcgcattata tttttctacc acttcgccaa acaattcctc atatttatct
      781 gctagattcg tctgctctaa ataaatattt tcatccgatt tcgtgaagtc aatgtgctta
      841 tgccaatcat tggttttctg gcgatcagtt aactcacgat tattatgaga aatacttgta
      901 ttcttcgttc cttttttaaa tgaaatacta aaccccaagt taaaaccctc cttcgcgctg
      961 tcgctaataa aaagattgta aacctttctg ttactctttg tcaaagagta acattttcct
     1021 aattgtgaca tggatgtcac aattccaaac gctctccgag ggttttcgct gtcacaagtg
     1081 acaacgaaaa aaggagccga aagcgtatca cgctatcaac tccttttatg ctttattttg
     1141 cttcacgcaa aataatcgct tatcgtaaat tcacgtcatt aaatattgac gtaaatttac
     1201 tctcactcat ctgacttttc ttattcggta tcactccgaa taagtcgtca gattcatcgg
     1261 gataatctcg ttcgcttggc gctgattttt catcagcaag ccactcatta tccactgttt
     1321 tttcgtgttg ctgatcgtca gaaattgacg ataaccaccg ctcaaaatca gcaaaagttt
     1381 caaacccaaa acgcttgcga agagccttgc ccattgcagc attttctttc tcaatcaagc
     1441 gttttttctg ttggcgaagt ttcgcttgtt ctttttttaa tttttcttct tgcgctttaa
     1501 tatctgctaa agtcattccc ataaactaca tctccttttc tttggaatta ataacggaaa
     1561 tttggtttaa ctgcataaca tgactcatta atttcaacca aaattctttt tctgattcat
     1621 cagttgtttg cgctaaatgc ttatgaacaa attcattagt caaaactgaa gattttgcta
     1681 gatcagcatt gataaccgag ttgagaacat ccataattcg ttcaagcatt tcactataat
     1741 tatcttcatc cggcaaattc aaataatcag ctaaccgttc atacatcaat tcaattctat
     1801 cgactgtttc ttctctcaaa tcaattttat ccatgtcaca cctcacttcc agaattgcca
     1861 ccattttcga ttttttaatt cttgattttc tagtaacaac tgatcgtttt tttgttgctg
     1921 aagcaaagtc aatttttgtt ggtcacgaat catttccgtt aattcagaaa tatgcctatc
     1981 tttttccgct aattgttcac gcaaaaattc attttctttt tctaacggga aatcattgcc
     2041 tgcatcattg accggtaaat caccagtcaa ttgaccacct tttgaccgat aaattgaccg
     2101 cacaacacca atcaattcct tgtctaacat gataacccca ttatctttat accgagcaag
     2161 gtcataacct gcattgacca gttgattgcg cactgtattt ttagatatac ccaactcatc
     2221 agcaaggttt tttatggtca ttttttctgg caaatcagtc attatcttct ccgttacttt
     2281 cagctacccc ataaacatat ttaacagcat gcttagggat aatgattggg gaatcaattt
     2341 tattattttt caaaatcaac tcatactgcg ttacatcaag taacttaccg gacatttttt
     2401 tgctatccaa aaaaacaacg acaacgttat cacctgttaa attcaccaac caatttttct
     2461 taccaaaatg cttttgtttt tctgccatta ctattcctcc tctgtcacat caagtccctg
     2521 taaccaagta tccaattctt tttttaaatc atctgctgat tcttcaaatg tttctcgtaa
     2581 atgattcgtc aactcatcat tttgactttc tcgctttaat aaatttcgat atttatattt
     2641 cgagtttctt gacaaaatcg cccaattaga atgattagct aacaatccat caatcataac
     2701 tttatccgta ggttttatat cagttggtaa aaaatgtgga ctcacaaagc tatccaaact
     2761 ttctttcacc atagcatacc attccgtagc tttaccacgt cttaactgca tttctaaccg
     2821 ccaccaagta ttaatttctg aaggcaccac ctttcttttc atttcttgct ctattttttt
     2881 attgtacaaa cgaatttgcc gctcgcttgc ccgactaccc caatacaccg tttccagttt
     2941 tcctgagcgt ccataaatta atttaaatga aactggatca ataattcgat attgcgtgat
     3001 aaactcatca ggcacatcaa taagatcgca ggcaacatcc gctcgtgaaa aatggggctc
     3061 taaaaataaa tcatgaataa aatttttcat ggaatccgct aaaaatcgac tgattttatt
     3121 aggattaaaa tcaatccgcc cttttccttc ttgccattta gaaatttcaa tataggcaat
     3181 attttcgtca tgttggtcta atagctgcca accattattt ccaaccgctt ttaaatagcc
     3241 attgccttca tttacccgca taagctgttc aaaatctaat attaatacat catgttgagt
     3301 atgataaaaa atattttgat tcaactttcc aacaatggta attcggtcaa tcgaccaatt
     3361 taattttata gtttgtacca aagtttgaac atttaaccgc ctgttagaca gggctgttaa
     3421 tttttcccac tcattcacga ttgttctttt tttactcata ttcagttcct cctcaaagaa
     3481 aaaagagatt tttggaaaga atttccagag ttcacgtaac tctaaaaatt cataaccatc
     3541 aatctctagt cttgaagaaa cctaaaaata ttcgtataat gaatttgagt tacggaacta
     3601 gtgtggttag tttccgtaaa ggtcgagaac gttggtagcg ttgctcgact tatttttttg
     3661 ctctttttcc agttcatttg tttgctaact caataatagc acttctcttt agctatgcaa
     3721 ataccaaaaa aataaaaccg caccctttga aaagggcgcc cccaaacttc gctaacgcta
     3781 agttggccaa aaaatggact tgccattttt tagcaaatgt cagcttcagg ggctagtggt
     3841 ttagccgtca actccttatc ctcggcacaa accgcctccg gtaccgttga ctgcacatcg
     3901 tggctaccac tcaatctcat tctcagaacg ctctaattta tgctgtgaga cgttttgacg
     3961 ataaaataag gttttctcct gctctaa
//
LOCUS       DACDTH010000139         3837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDTH010000139 DACDTH010000000
VERSION     DACDTH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.144"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..621
                     /locus_tag="IWK54_002865"
                     /pseudo
     CDS             1..621
                     /locus_tag="IWK54_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1069..1515
                     /locus_tag="IWK54_002866"
     CDS             1069..1515
                     /locus_tag="IWK54_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594911.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="IWK54_002867"
     CDS             1517..1654
                     /locus_tag="IWK54_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9594912.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="IWK54_002868"
     CDS             1691..1984
                     /locus_tag="IWK54_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594913.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="IWK54_002869"
     CDS             1981..2205
                     /locus_tag="IWK54_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9594914.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..3221
                     /locus_tag="IWK54_002870"
     CDS             2202..3221
                     /locus_tag="IWK54_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9594915.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            3410..3483
                     /locus_tag="IWK54_002871"
     tRNA            3410..3483
                     /locus_tag="IWK54_002871"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3444..3446,aa:Ile,seq:cat)
BASE COUNT         1323 a          669 c          786 g         1059 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     3181 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     3241 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     3301 catatcttca ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg
     3361 ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc
     3421 tcagttggtc agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt
     3481 acattaacgt agccatttga atcgttctgt gttagaattt tttgaagagt attatacaag
     3541 ctaaagcttt tcttcattgc cactcaaatg agtggctttt ttatgtatcc ttttatggat
     3601 taatgaaagg atgtttcaca tagttatatt tctgtatatt tgaaaagttt tactttgatt
     3661 tttaaataga aagacatttg ggttatattg tgagataata ataaagaaga gtttaaagcg
     3721 ttccccaaaa accactgccc cataagtgtg ttacgcttta aactctttta tatttgaagc
     3781 cattaaaaag cataccatat ttttgaaaaa aagtgagaaa aaaggcttat aattgga
//
LOCUS       DACDTH010000140         3803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDTH010000140 DACDTH010000000
VERSION     DACDTH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.145"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(252..2249)
                     /gene="tkt"
                     /locus_tag="IWK54_002872"
     CDS             complement(252..2249)
                     /gene="tkt"
                     /locus_tag="IWK54_002872"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9594916.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2351..2602)
                     /locus_tag="IWK54_002873"
     CDS             complement(2351..2602)
                     /locus_tag="IWK54_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9594917.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2749..3372
                     /gene="lexA"
                     /locus_tag="IWK54_002874"
     CDS             2749..3372
                     /gene="lexA"
                     /locus_tag="IWK54_002874"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9594918.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3552..3770
                     /locus_tag="IWK54_002875"
     CDS             3552..3770
                     /locus_tag="IWK54_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594919.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1059 a          809 c          700 g         1235 t
ORIGIN      
        1 gaaaatcact aaattaccaa acaccattag aagttttttt gagttacgta aatggaaagt
       61 tttgtctcgc ttaatttgac aaataatatt ctcaagaaac acaaactaat ccgaagaatt
      121 ctttgttttg tcatttaaat gtaacatagc cgaaaacgaa aagtaatatt gatttattct
      181 tctaacacat aaagaacagc tgtctccaac ttgcagacag ctgttcttat cagtcaaaca
      241 attgcttgga tctatgatag ttgattgtac gtattcacga cattttctac tgtaaatcca
      301 aattcttcta aaattttagt gccaggggca gatgcaccga aatgatcgat tgtaatcgtt
      361 gcgccctctg ttcctacgta gcgttcccat ccaaatgatg cagcagcttc gatagccaca
      421 cgtttcttca ctgatttagg aagtacactt tctttatatt cagaagattg tttttcaaag
      481 aggtcaaagc ttggcataga aacaacagaa acatctttgc ctttttcagc caattctttt
      541 tgagctttca cagccaaatc aacttcagaa cctgttgcaa tcaaaatacc ttctggtgtt
      601 tctccttgtg atggagaaag aacatacgcg ccttttttca ccatttcatc tgctacttct
      661 tttgtagaag gcaatactgg aaggttttga cgactcaata caaggatagt aggcgcatct
      721 gttgtctcca tcgctacttt ccaagcagca cgtgtttcat ttccgtctgc cggacgaatc
      781 acttgaacac caggcatgca gcgaacagat gctagttgtt caatcggttc gtgcgttggt
      841 ccgtcttctc cgacagcaac cgagtcatgt gttaagacgt aaacaacagg agtatgttgg
      901 attgctgcta aacgaactgc tggtcttaga taatcgacaa agacaaagaa tgttccaccg
      961 tagatacgag ttccgccatg aagttggata ccattcatag ccgaagccat tgcaaattcg
     1021 cggacaccga accagatgtt gcgtccttca taatgttcag gagtgaaatc tttatctgca
     1081 gccaccattg tattattgga acctgacaag tccgctgaac caccccagaa gcttggtata
     1141 gctttcgata attcttggat gacttcttta cttgaaacac ggcttgcttg actgcttcca
     1201 acttcataag ttggcaattc tgcatcccag ttttctggta gtttgccatc aaaagcatct
     1261 tcaaattgtt gtgctaattc aggataagct tttttataat tggcaaacat ttcattccat
     1321 gcatcttcag ctttttgtcc ttcttctatc atcgtttgat ggaagcgggc agctacttct
     1381 tctggaacag tgaaatctgg atactcccaa ccataaactt ctttagccat cttgatgcca
     1441 tcttcaccaa gaggagcacc atgaacagca gatgttcctt ctttaggagc accatagccg
     1501 atgactgttt ttacttcgat caatgttggt ttgtctgttt cagcttttgc ttcttcaatc
     1561 gctttagaga ttgcttctag gtcatttccg tctttcacta agatatgttg ccagccatat
     1621 gcttcatagc gagcaccaac gttttctgtg aaagcttttg aagttggacc gtctagtgaa
     1681 atatcgtttg aatcgtataa aacgatcaat ttccctagtt tcatatgacc tgccatcgaa
     1741 cttgcttctt gagatacccc ttccatcaag tctccgtcac cgcatattgc atacgtatag
     1801 tgatccatca cattaaagtt ttctttgtta tacgttgctg caaggtgcgc ttctgccatt
     1861 gccataccga cagccattgc aatcccttgt cctaatggcc ctgtagtggc ttctacacca
     1921 tctgtatgat gtacttcagg atgtcctggt gttttagaat cccattgacg gaattgtttc
     1981 aaatcatcga ttgtcacttg atagccagct aaatgaagca agctatacaa cattgctgac
     2041 ccatgtcctg ccgaaagaac gaaacggtcg cggtcagccc agttttttga tgtcgttgga
     2101 tttactttca ggtgttttgt ccataacgca tatgccatag gtgctgcgcc catcggcaat
     2161 ccaggatgtc ctgagtttgc tttttggact gcttcaatac ttaatgtacg aattgtgtta
     2221 acgccaagtt ggtcaatttt gtcaaacaaa ataacccctc ctattaattt aggttttttt
     2281 tacctttatt tcttacactt tcattctata agatgtcaca cgaaaaagca atctttttca
     2341 gtatttttta ctaaaagttg tcatttcggt tgtgtaagcc ttttttcttt tggatttctt
     2401 ttaatttttc cggggtcacg tcattgccct caggatcgac gactttcatt ccttcgatat
     2461 gatgacgcat accggaacga aaagcttcca aatactcttt gcgcaagttg atttgttctt
     2521 gttcttcggc ttttgagaga ccattttcct tctttttacg tgcaagttca ttgatccgct
     2581 ctattttttc aggtgacagc atgctcagcc ctccttttac tctccttcat attatataaa
     2641 aactgaaaaa aaaacaacta gataaactaa ataaaatacg aacattcgtt tgatttagcg
     2701 ctttttatat ggtactattt atttatcaag tgaaagaagg tgtaccttgt ggcccgacaa
     2761 actgaatcaa gacaaatcga agtcttacga tatatccatg aacaagtaac ccaaaaagga
     2821 tacccgccga ctgtccgtga aatcggggaa gctgttcagc tttcttctac ttctactgtt
     2881 cacggtcatc ttgcacggct tgaaaaaaaa gggtttatcc aaagagatcc gacgaaacct
     2941 cgtgcaatcg agttgacaaa acaaggttta gaaaagattg ggattcgtcc aacaactatt
     3001 ccagtacttg gcgtcgtaac agctggagaa cccattttag cagtagagga tgcttcagac
     3061 ttctttccaa tccctcctaa tctttcttcc gaagaaggca gtctctttat gctgacaatt
     3121 cgtggagata gtatgatcaa tgctgggata ttggatggtg acaacgtgat cgttcggaag
     3181 caagaaactg ctcaaaatgg cgatattgtc attgcaatga ctgccgagga cgaagcgact
     3241 tgcaaacgat tctataaaga aaaaaacttt ttccgtctgc agcctgaaaa cgacttgctc
     3301 gaaccaatca tcttagatca agtatctata ttaggacgag tagtaggact ttatcgtagt
     3361 catatctatt aattgctact aattaaaaga aaacttttta aaaagagttt gagacgttgg
     3421 tctcaaactc ttttttcgcc cgggttcttc ttctttcaaa tttttatcac ttccctcttt
     3481 acaaaccgaa caaatattcg tatactaaat atacgaacaa ttgttcgcat aaattaaaga
     3541 ggtgagaatt catgaaatca atgagacgtc ttggcttagt attaactgtt ttgtttttag
     3601 gtattattat tggtaatttc ggcatacgtc cagctttact tgtgtttgtt ccgttattta
     3661 ctttgtggtt catgttgtgg gatgaaaaga agtaccgtca atctgagcgg cgcagaattc
     3721 atgaagatca tttctatcat gagccctact acagatccag gcaatcataa taaggattga
     3781 ctgctaatta ttcaaaagac aag
//
LOCUS       DACDTH010000141         3681 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDTH010000141 DACDTH010000000
VERSION     DACDTH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.146"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            120..1094
                     /locus_tag="IWK54_002876"
     CDS             120..1094
                     /locus_tag="IWK54_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9594920.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1076..1537
                     /locus_tag="IWK54_002877"
     CDS             1076..1537
                     /locus_tag="IWK54_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9594921.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1587..2051
                     /locus_tag="IWK54_002878"
     CDS             1587..2051
                     /locus_tag="IWK54_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9594922.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2065..2814
                     /locus_tag="IWK54_002879"
     CDS             2065..2814
                     /locus_tag="IWK54_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9594923.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2814..3644
                     /locus_tag="IWK54_002880"
     CDS             2814..3644
                     /locus_tag="IWK54_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9594924.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
BASE COUNT         1239 a          486 c          711 g         1245 t
ORIGIN      
        1 aaattgttat aaaaaaattt ttttgaaaaa tagtattgca aaataaagca atttcataga
       61 taataggtat tgtaaagtta tatgacttta taatacttat tagagtgagg gataaagaaa
      121 tgagacaact acacaaagaa cagattgaaa cagcaatggc agcgttaaag gaaaagacaa
      181 ttgatcatgt atattttaca gcatgtgggg gttcacaagc cgttttaatg gctggtcaat
      241 atctatttga taaagaatta actgtacctt gtcatgttta tactgctaac gaatttaact
      301 atgctatacc aaaaggatta tctgaaaatt ctttagttat cacttgttcc cattctggca
      361 caacaccaga aactgtagaa gcaacaaaaa aagcaagaga atttggagca atgactattt
      421 gtttatctaa tgaagaagga tctcctctat gggaagcagc tgaatatcct gttcactatg
      481 attggggtaa agatgttgat gctagtgatt taaataaagg aattctttat ggtttattat
      541 ttaatatttt acttgtttta gatggctcta aaaaatggga aacttgcttg aatgaatttg
      601 ataagttaaa tgatcttgtt attgctgaaa agagcaaatt tgaaataact gctaaagaat
      661 ggggtaaaaa ccaaaaacga gataaaatta tttacacaat tggtagcggt atcaattacg
      721 gagaaattta ctccacagca gcttgctggt tcatggaaat gcaatggatt aattctaatg
      781 ctattcattc tggggaattt ttccatggtc cttttgaaat cactgattat gatgtaccat
      841 tcatcttagt gaaaagtatt ggagatacaa gatttttaga tcaacgggta gaagacttca
      901 ctaaaaaatt cagtaataaa ttaatggttt tagaccaagc tgattttgac ttagaatcta
      961 tagatagtgt agcgaaagaa tatattgcag ctgtacttag tggcgttgtt attcgccaaa
     1021 tggtagaagc gattgctttt gaaagaggcc attccttaga tgttagacgc tatatgtggc
     1081 aaatgaaata ttaattaata ggaggttgca aaagatggca aatcaattat ttcacattgt
     1141 gattacaaca cattctaccc tttgtgaggg ttatttaaag gcaacaaaat taattttaat
     1201 gaatgacgcc acaggtatta gtgctgttcc atttaaagaa aatactagtc cagatgattt
     1261 tgaggagagt atgaaagaaa taattagtca gcatcatttg caacctctaa ttattttaac
     1321 ggatttaatt ggaggaacac ctaataatgt ttctattaaa cagttgaaca acaatagaat
     1381 tcaaattatt gctgggatta atttacctct tttactggag ttattaatgg cccagtcagc
     1441 tggtaaatct atagaagaaa tagattttaa ggaattactg aataactctc aaaaaagtat
     1501 ggttcatatt aattcgcttt taaaagatgt cagttaaggt ggcaatttct attctataaa
     1561 ttttaaaaat tgaaggagga aatagaatga ttaaattagt gcgtgtagac cacagattac
     1621 ttcatggaca agtaatattt tcttggacta aacagtatga tattaaccat attattgtgg
     1681 ctgataacaa agttccagga gatccaatgt ctgtgatggc tttgacaatt gctaaacctg
     1741 ctgattgcaa attagacatt gttaaaatta gtgatgttaa agggattatg gaaacatccc
     1801 caaatgataa ttacatgatt ttaattaaag gacctaagga agcttttgaa ttagttcaaa
     1861 atttaccaga aattaaagaa attaattttg gcggtgtgtc taagaaaagc gagtccaaac
     1921 aatatggaaa ggcagtcttt ttaactcctg aagaattaga agccacaaaa aaaattcttg
     1981 atcaagaagt gaaaatattt gtgcaacaag tcccatcttc aagtattgaa aatgtagatt
     2041 ttagtaagta aggggggaaa ataaatgtta ttaaaagcca ttttattagg tttagttggt
     2101 gtggtagctg ttattgactc caggttaatt ggtcgacaaa atattggtag accactaatt
     2161 ttgagtacac ttgccggact tgtgctaggt gacttgcata cagggattgt tcttggggca
     2221 tctttagaat taatttctat gggattcgtc tcaattgggg cggctggacc tcctaatatg
     2281 caacttggta gtattattgc cactgctttt gcaattttaa ctaatagtag tacagaaaca
     2341 gccttaacaa tagctatccc agtggctgta gctggtgaat ttatgagtat tatcatgcgc
     2401 atgataattg ctcaatttgg acatgctgca gataaagcca ttgaaaataa taaatttaga
     2461 aaagctcaaa ttattcatat ttattggtca tttattttta atgcttttgt ttactttatt
     2521 ccaatctttt taacagtata ttttggtgct gacgttgtgg cagatttagt agataaaatt
     2581 ccagaaatga ttacagacgc cttgactgtc tctggaaata tgctttccgc attagggttt
     2641 gcaatgctat taagcacaat gttgagtaaa aaattatatc cttactttat ttttggattc
     2701 tttattgttg cctattcagg tctttcacta attggcgtga caatttttgc agctctgatt
     2761 gcctttgtaa tggatcaagt taaatatgga aaaagagaag aggcgcatgg ttaatggaaa
     2821 atagtacaga aaaaaaacgt ttaatcacaa aaaaagatct taataagatg tgtcttcgtt
     2881 catggacaat catgtgttcg tggaattatg aaagacaaat gcatatggga tttatgtatg
     2941 ggatggcgcc aattttaaat aaagtttata aaaatgatga agagaaaaaa agagaagctt
     3001 atactcgtca tatggcattt tttaactgta cgcctcaaat gacttcattt attatgggac
     3061 tttgtgcttc tatggaagaa caaaatgctc aagcagaaga aggaaacttt aatactgaat
     3121 ctatctccat gattaaaacc agtttaatgg gtccttttgc tggaattggt gattcatttt
     3181 tccaaggtac tttaagaatt attacatttg ggattggatt gtcttttgct aagcaaggta
     3241 gtattttagg accgatttta gctgttggcc tttttgctat accttctatt ttagctgttt
     3301 tttacggcgg taaattagga tatgagtctg gcaataaata ccttactaaa ttgtatcaag
     3361 aaggtcttat ggatcgagta atgcaatttg cttctatcat tggtttggct gtagtaggtg
     3421 gaatggtggc tagcatggtg ccaattgtca cccctttaaa attcactaca gggggaacag
     3481 aacttgtttt acaagactta cttgattcca ttattcctgg attattacca cttgtatgta
     3541 ctttgggaat atataaattg attaagaaaa atgttagtac aaatatgctc ttattaggtt
     3601 gtattttggc agggtttgtt ttaagtataa taggtatctt ataatgatta aggctctaat
     3661 ttttgatatg gacggagttt t
//
LOCUS       DACDTH010000142         3568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDTH010000142 DACDTH010000000
VERSION     DACDTH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.147"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            570..1154
                     /locus_tag="IWK54_002881"
     CDS             570..1154
                     /locus_tag="IWK54_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9594925.1"
                     /translation="MNKLFMISGENGTGKTTLMKLFTKQLPVSNNQLFVNGIDINMID
                     NESLRRLISIATQIPYIINSSVKENILMGTKNYDKKKFSKLIELLGISKFMTKLGITL
                     DDNIYDEKILLSGGEKQKISLARALIKSASVIILDEPFRNLDNETRDNLRDYILNTKK
                     EVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            1147..2175
                     /locus_tag="IWK54_002882"
     CDS             1147..2175
                     /locus_tag="IWK54_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9594926.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            2231..2449
                     /locus_tag="IWK54_002883"
     CDS             2231..2449
                     /locus_tag="IWK54_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594927.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            2520..3335
                     /locus_tag="IWK54_002884"
     CDS             2520..3335
                     /locus_tag="IWK54_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594928.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            3399..>3568
                     /locus_tag="IWK54_002885"
     CDS             3399..>3568
                     /locus_tag="IWK54_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9594929.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKK"
BASE COUNT         1402 a          395 c          600 g         1171 t
ORIGIN      
        1 tgatgggagc tatggtgtta cagtataatt tatttcaatt accgcttaag aaaacaatcg
       61 agaatacaaa aagtgttaat caaagtatta caggagaatt gaatgcacat tttgatcaat
      121 tagaaattat aagcgcatat aattttgaga atatctctat tttaaaattc gattctagat
      181 ttaaagaatt atttaatgtt ttcaaaaaac aaaataaatt actaacactg ggaagctgtt
      241 tggcacaggt tattttccaa ttatggattg gaggaatatt tcttattgat gttattttat
      301 tagctaaagg aacaataagt attactaatt ttgtaatagt atttatgtac gctaacatgg
      361 tccctacttt atggactact ttaattagta ttatttggaa atggcaagac tattcagtgt
      421 cattaaatag atattacgaa tattattcaa ataacaacaa agtggctggc acaaaaaaaa
      481 taaataaaat aaattctatt actggtcaat cattaaattt ttcatataac aaaagaaagg
      541 tattaagcaa tataagtttt tcaactgaaa tgaataaact attcatgata tcaggagaaa
      601 atggaacagg gaaaacaaca ctgatgaaat tatttactaa acagttgccg gttagtaata
      661 atcaattatt cgtcaacggg atagatatta atatgattga taacgaatca ttaagaaggt
      721 taatttcaat cgcaacacaa ataccatata taattaatag ctccgtgaag gagaatattt
      781 taatggggac taaaaactat gataaaaaaa aattcagtaa attaatagaa ctattgggaa
      841 taagtaaatt tatgacgaaa ttaggtatta ctttggacga caatatttat gatgaaaaaa
      901 tattattatc tggaggagaa aaacaaaaaa taagtttagc tagggcgctt attaaatcag
      961 cttcagttat tatattagat gaaccattta gaaatttaga taatgaaact cgcgataatt
     1021 tgagggatta tatactaaat acaaaaaaag aagtttgtta tataattata actcatgatc
     1081 acattctaga taattttttg gattcaaatg atgaaatatt atatttaaat aaggagtatg
     1141 ataataatga atagcaaaaa acttcagatg ggtgaaattg ttagaggtcc tagtcaaata
     1201 tctattgatg ttacatataa atgtaatttt agatgtaaac attgttacaa tgcgagtggt
     1261 ggagacaatt gttttatgga taatgagttg actgatgatg aactgttaga acttacaaaa
     1321 gatatatgta atttaaagcc atataatatt tgtttctgtg gaggagaaac tttactgaga
     1381 aaaaaaatag ttttatcttg tgccacttta ttaaaagagg caggtattcc taatattgct
     1441 attgttacta atggatcatt agtaactcag gatgttgcaa aagaattatt caaagcaggt
     1501 attaatcgat ttcaagtaag tttagatgga aaaaatgcta gtagccatga agcacttaga
     1561 ttatatccaa acagttttga aaaggcaatt aatgctatca aattatttag agaattgtcc
     1621 gaagaagtgg atgttgcttt ttgtcctact agttttaatt acaatcaatt acgagaagta
     1681 tatcaactag taaatactct taaagtgaag tctttaagaa ttcaaccatt tatgcttagt
     1741 ggtaggggaa tgcaaaatag ggaaatagtg ccaactgttt ctcaatatag aaaactagtt
     1801 tctgatatta actatattaa aagaaatgaa gattatacca agatagattg gggagatcca
     1861 attgatcata tcatccgaca cagaacttta ttgaaagatt tttcgaattt tgtttctatt
     1921 aggtcagacg gaggattaat ggcatcacct tacttgccaa ttcaagttgg aaatattaaa
     1981 tgtcacagtc tttctaaata ttggcgagca ggactttata aagttttttc tttagattta
     2041 gttcaagata tagctactca agtttcttca atacaagata tgagtaaaga attaaaaaaa
     2101 ggacatatta aatggcttaa tgaagatata gaaattgata ttctcgatga atacaaacat
     2161 gatattttaa tttaggttta tcattggaaa gtttagataa acaaagtact tttagaaagg
     2221 agggaaaaaa atgatttctg aacctcaaat gaataatatg tatgaaagcc caactataat
     2281 gggagctggt gaagctgcgc caagagcttg ttcagcaatt atggtgtgtg tgttattagc
     2341 atctgcagga gtaggtatcc aagcaggagc ggtgtatact tatgctgtag ttgctacagt
     2401 agctgtagct gcagccgcag tttggaaaac agtaggatta gttggttagg aattaatagg
     2461 agactaactg tctcctattg attccaatga ttaagctaag gaggaatttt aagaaaataa
     2521 tgaaatacaa taatttaaaa ccgaaattac ttaatgagaa catagaatat ataagagaag
     2581 aaaattatgg atatttaacc atagttccca aaaatggaac tgaattgaaa gataacgttt
     2641 tcaatccaac agtaagatat atattggcta actgtactgg aaaaaacagt gtaaaaaaaa
     2701 tagcgaatgg tttaaaaaag aagtttccta gtttagaata tgaaaaattc tatcaagatg
     2761 ttgtaggaac tattaaattt ttcaaacact ataatattat tgatatagga gattatagta
     2821 tggaagaaaa gaaaatatat aaaattgaac aaaatttaga attagaatta ctaaatgaat
     2881 cacattatga agaaatttat aattttatca cagatcatcc tgaaagtatg tattttcatg
     2941 aagatattta tcatcaagga aatagtaaag aattagaagt tgtccttaga tataatttat
     3001 tttcgtttac gagtgaattt tactttttaa gacataaaga gagaattgta ggaatggttt
     3061 ctataattgt taatccattt caaaacttat cctacagtat agtaaattac ctcatatgtg
     3121 agcctgcata tactacgact attttgacaa cagtattaga aaatattaac gaagtttcaa
     3181 ttttagaagt aaagaagcta gtgattcatt tgctaaattc aacagaagat gaatcactaa
     3241 tcccctattt aaaagattca cattttttta aagaagcggt actatctaaa gaatatagag
     3301 agcatgatgt tttgagatac gcttacattt attaaataaa atttctgata gtattttaat
     3361 aattcggatc atatcataag atataggagg gaaaagaagt gattttattg attttgtctc
     3421 tcgtattact agttgtatct aatctaatga aaaataatcc tcctacaaaa attaattctt
     3481 tttatggctt tagaactaaa aagtcaatga aaaatgaaaa aagttggcat ttagcacaaa
     3541 aattgttggg ggatttttca aaaaaaaa
//
LOCUS       DACDTH010000143         3482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDTH010000143 DACDTH010000000
VERSION     DACDTH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.148"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(74..1078)
                     /locus_tag="IWK54_002886"
     CDS             complement(74..1078)
                     /locus_tag="IWK54_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9594930.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
     gene            complement(1149..1445)
                     /locus_tag="IWK54_002887"
     CDS             complement(1149..1445)
                     /locus_tag="IWK54_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594931.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(1438..1731)
                     /locus_tag="IWK54_002888"
     CDS             complement(1438..1731)
                     /locus_tag="IWK54_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594932.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1877..2428)
                     /locus_tag="IWK54_002889"
     CDS             complement(1877..2428)
                     /locus_tag="IWK54_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP9594933.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2602..2901)
                     /locus_tag="IWK54_002890"
     CDS             complement(2602..2901)
                     /locus_tag="IWK54_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP9594934.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(2928..3236)
                     /locus_tag="IWK54_002891"
     CDS             complement(2928..3236)
                     /locus_tag="IWK54_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9594935.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
BASE COUNT         1005 a          679 c          484 g         1314 t
ORIGIN      
        1 aaaaaaactg attctaagaa aattaaataa tttttctcaa aatcagtttt tctttgataa
       61 ataatatttt caattattta atcatttgaa aattgttagt cattccaata cgttgagaaa
      121 ttcttccatc tgtataaaca cggaattgtt ttccaccagc cattgttgta tttccattat
      181 catccgttac aatattaatt gaatagtagc ttccattttc atcttgcatt agatctttca
      241 tatattcatt tgtgctaatt aaattagtat ttgatgcatt tttatcagga gaatcataca
      301 accataaagc tttattattt agtacagttt ctttagcagc ttcccaactt gaaattgctg
      361 agctacttgt agattgtgtc gttgtagttg ttgcttgttg agtagattct tctactactg
      421 gagtttgagg agtttgtcca ttcacaatat caatgaaacc ttgttttaac tgttggttct
      481 gagtttcatt gaagtacatg tagtttttat cattgccttg attttgttgc gttaaataaa
      541 ctttaggttg tccattttga aagccaaaga aatatacatg ctgccctaaa tattctccag
      601 tttctacatc agaataaact gctactaaat tgaaatctgc atgaccagtt ccatcttctg
      661 accattgtac ggtaactggt gcttcattga ttgccatttt ccaatttcca tcaataactg
      721 cacttggtaa aggcgtacca tacagactta cattgttctg aggattgtag cttttatatt
      781 cttgacctaa tgtctttccc cattgtggga caaaaattgc tagttgagta gatttctcac
      841 tatcccacaa agtattttta acctcttcag aagaagattc aatgattgaa ctttcacttg
      901 tacttgcttc cacagattgt ttcatttctg ttgtagtttg ttcagttgat tgcgttttac
      961 tttttttagc ctcaattgtt gtatcagttt ctacagtctc tttagtcctc gtattctcag
     1021 atgtacaacc agtgaataaa atccccattc caattagtaa taacaaccat tttttcattt
     1081 tatttccccc tttcaatttc tccctcgcgt acttatttta tcagtcaatt aatctcaatg
     1141 gtgttcattt agttaatcct ttcccaagaa tgtcgatagc cagcatgaca acgatctctt
     1201 ccttgagggc tttcagatgg attaaaacta gtagaaattt cccttccaca tcctctacag
     1261 cgataagttc tcattatttt tgggctatta gtattatttt tcttagttag agctatgagt
     1321 ccaactatga ttaccccaac tgtcttaatt acttttcctt tatctatatt cggatgcgca
     1381 agcatatatt tatataacca atatccaccg taaattaaac cacctaatac aatcagctta
     1441 gccatgtact cgtctccttt ctttttgttt aattactttt tgaatccatc ttgcagagaa
     1501 accataaact tctgtcaatt gacggatatc tacagttttt tcctgttcta tcatgttttc
     1561 gattcttttt ctaactaact cacgatcgta cattttcatt ggaaaagaaa tctgttgccc
     1621 tcgaaaagca agataaaatt gatagagcaa ttctgaatcc agttccattt cctcaaaaaa
     1681 gtcattcata cttttataag agttactcaa acaagtttca tcaatctcca tctacacacc
     1741 ccctgttatt ttttacataa tgaaaatagc atattactgg gtacattgat aattcatata
     1801 acatgtatca tcacatgtat ttttaaagtc aaaaagcatg taaaactact tataagtaac
     1861 ataagaaaat aaaatgttat ttttcttgaa ataataattg actatctgcc aattgccatt
     1921 cattacgaac atgaatactt agtgggtggg ataacttacc ttctgcattt atcaatactt
     1981 tggtcatctt tttttgtttc gcactcaatt gatcgtaaat ggtcttcgct tgttcataag
     2041 ccacttgact cgtcgctgta atcgaaccac aagcaaaaat aatggtgttg attttctttt
     2101 ctgtgaaaac agttgttaat atttccaaag attgttcatc atttccacga ggaaataccc
     2161 gaggactttt cgcttttgta tacgaaaaaa gattcacagc aataaaacca gtaaatccta
     2221 attgtcggat attcttttcg attaataaca tactcaaatc attcgtaaat ggttctgtat
     2281 ctgttggacg aatagtaatg actaccgcta atttatcttc cgcttctttt cctttgatcg
     2341 cccattgtcg tttgaaaaca tagcgatggg tatgatgagg atctcgccaa acttctgttt
     2401 gtgtggtcac taattctttt tcaatcatct ctttttctcc tatgctaatt tttgttcaag
     2461 caattcaatc accattgccg ttataagtgt caatactagt tgaaaatatt tcatattctt
     2521 tctccttttt tcttcagaat ttgatgaatc gctgtaagga tatcttctaa acatttcgca
     2581 atcattctca aataatcgat cttattcatt cgttcctcca atctgactcc aaaccaatcg
     2641 tccttctacc agtaacccac gaacaaattc tcgctggctg gtaatatctg taatgaaata
     2701 aactacatag tcttttccat cttctggtgc gcaataaata taaagatgtg tcatcactaa
     2761 acgtggtaga gtaacatctt caggaagcat acccatttca atttcctctt tttccatcgt
     2821 aacggattca tagccccact cttcaggagt agtgactagc tgtgcacact tttccagtac
     2881 ttcgatagcc gtttctaaca agtcctttcg ttcctttctt ttagatatca gtattcttct
     2941 ggtaataaca tcgtttgata ctcacccgaa tcaatgaccc atattttttg agacactgat
     3001 ttcaaacgaa gcttaggtga aatcagatac tcttttcttg cttctggttc ttcttgacga
     3061 tgaaccaact tttgatttcc tttttccgta gaaaattcaa agatttgaag atagtctaaa
     3121 tgaaattgct tttgaacgtt ttgatcgatt agattccaac ataaagcttg taattccacc
     3181 ggcaaactgt ccattactcc ctttgtgaca taacgttgtc ctttattttg aaacatttct
     3241 tttccctcct ttgccataaa aatagcccaa tcttctatta aagaagattg ggcttcatgg
     3301 atataatata tatttgatat tcaatttgat tcattttata ctatctgatt tgtcaaatta
     3361 agctagacaa tatgtcttta tctatgcagc tcaaaaatac ttccaatggt gttcgataat
     3421 ttaatgattt tcttggaata tggtttcttt tcgaggcaac ggattgaata aaggtttcat
     3481 cc
//
LOCUS       DACDTH010000144         3329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDTH010000144 DACDTH010000000
VERSION     DACDTH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.149"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..2046
                     /locus_tag="IWK54_002892"
     CDS             <1..2046
                     /locus_tag="IWK54_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9594936.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPTLPEGT
                     TVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGE
                     KVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            2180..>3329
                     /locus_tag="IWK54_002893"
     CDS             2180..>3329
                     /locus_tag="IWK54_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9594937.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKN"
BASE COUNT         1316 a          474 c          681 g          858 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aaggcgaaaa agtagaagtt
      421 ggaggcacag tagatctaac agataatgta accaacctac caacattacc agaaggaaca
      481 acagtaacag atgtcactcc tggtggtacg attgatacca acacaccagg taattacgaa
      541 ggggttatcg aagtaacgta tccagatggt acgaaagata cagtgaaagt tccagtagaa
      601 gtgacagaca atcgttccga tgccgataaa tatgaaccaa cagtagaagg cgaaaaagta
      661 gaagttgggg gcacagtaga tctaacagat aatgttacta acttgccagc gttaccagaa
      721 ggaacaacag taacagatgt tactcccggc ggtacaattg ataccaacac accaggtaat
      781 tacgaaggtg tcattgaagt aacgtatcca gacggtacga aagatacggt gaaagttcca
      841 gtagaagtga cagacaatcg ttctgacgcc gataaatatg aaccaacagt agaaggcgaa
      901 aaagtagaaa ttggaggcac agtagattta acagataatg ttactaactt gccagcgtta
      961 ccagaaggaa caacagtaac agatgttact cccggcggta caattgatac caacacacca
     1021 ggcaattatg aaggtgtcat tgaagtaacg tatccagacg gtacgaaaga tacggtgaaa
     1081 gttccagtag aagtgacaga caatcgttct aatgcagata aatacaatcc tgaaggacaa
     1141 aaagtgacta cagacttaaa taaagagcca gatgcatctg agggaattaa gaacaaagaa
     1201 gatttaccaa aaggaactac ttatgcttgg aaagagaaag tagatgttag cacagctgga
     1261 aataaaaaag gtactattgt agtgacatat ccagatggat catctgatga agttgaagta
     1321 gacgttacag tgacagatac tcgttccgat gcagataaat ataatcctga aggacaaaaa
     1381 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
     1441 ctaccaaaag gaactactta tacttggaaa gaaaaagtag atgttagtac agctggaaac
     1501 aaaaaaggta ctgtagtagt aacatatcca gatggatcta aagaagaagt agaagttact
     1561 atttctgtag tagataaaaa agcaccgaat aaacctcaag ttgatcctat tacagagggt
     1621 gaccaaattg ttactggtaa aactgaacca aatgcagagg tgacagttac attaccagac
     1681 ggaagtcaac accatgggac agctgataaa aatggtaatt ttacagttaa agttcctaaa
     1741 ttagaggcag ggacaaaagt gatagtaact gcaactgatg aatctggtaa tactagtgaa
     1801 cctactaatg tagttgtttc ttccaacaaa aaagacagtg gaaaaaacgg tagtaaaggt
     1861 agtaagactg acaatcaagg tagcaacagc aatcaagaca aaaatagagg caaaagccag
     1921 tcctcaaaag tttttcctaa gacgggagag agtgacagta atatctttac tattagcggt
     1981 ggtttaatac tgttaggaac gttaggatta ttagggtata aaaatcgaaa aaaagagaat
     2041 gaatagaaaa tcaatttctt aagtcttctc tgagaagagg aagaatcttt ttcttcctct
     2101 ttttgctttt ttatgatggg aaataagtgt tagttaaaga tgaatatact ttttatagaa
     2161 aggaaaagaa tgtaattaag tggaaaaaat agtaattatt ggttcaaacc attcagggct
     2221 agccgcagct cgatttctaa acttaagcga ttcaaattat gaagttattc taatcgataa
     2281 aaaatcagaa attagttact tggaatgtgg gacatcgtta tttataaaag gagaagtaag
     2341 taatttagaa gatctttttt atcaaaatag aatgacacta aattctgaga taaaaaagtt
     2401 atttctagag acagaagttg acaaaattga tattcaaaat aaaaagatta gttgtgtaaa
     2461 aaaagatggt acaagattct ctcaagaata tgacaaagtt atattagcta caggttcaaa
     2521 gcaaattatg ctagatatac cggggaataa tttagctaat attttttcga taaagaattt
     2581 tccaaatgca aaagagattt ttaaagcttt ggatgatagt actattaaaa aagtaggaat
     2641 tattggagca ggatatattg gagttgaaat tgctgaagcc ataagaaaaa gagggaaaga
     2701 agtatatctt ttcgatgttg cagatagagt gttgtcgact tattacgacc gtagtttttc
     2761 agataaagtt gaagaaatac tatcaaaaaa tggaatacat ctttgtctat cagaaagtcc
     2821 taaaaaatat ttaggaagaa aaaagattga aaaaatagtc actacaagtg gtacccaata
     2881 ctctatggat atggtcgtac aagctatagg atttctatct aatagtccaa ttggggaaaa
     2941 ggaactcttg agagatgtta gtggagcata tctaactaat gaataccagc aaacaaatat
     3001 aaaagatata tatgctattg gggattgtgc tacaactttt tttacgtcca ttaatagaag
     3061 tagtgttgat ttttctattt ctaatgctct aagaacagct tatattgcaa ctcaacatat
     3121 taacaatcaa gattttacac cttctggttc ccaatttaca aatggctttt ctatatttaa
     3181 ttacaatttt tacgcagcag gtttgaattt aaaaacagca cgattattaa gtgagtctat
     3241 agattatata gaaatagaag atttcattag acctgctttt ttgagaaaaa atagtcgtgt
     3301 aaaattaaga attagctttg aaaaaaata
//
LOCUS       DACDTH010000145         3126 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDTH010000145 DACDTH010000000
VERSION     DACDTH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.150"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..940)
                     /locus_tag="IWK54_002894"
                     /pseudo
     CDS             complement(1..940)
                     /locus_tag="IWK54_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1161..1523)
                     /locus_tag="IWK54_002895"
     CDS             complement(1161..1523)
                     /locus_tag="IWK54_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594938.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(1527..1835)
                     /locus_tag="IWK54_002896"
     CDS             complement(1527..1835)
                     /locus_tag="IWK54_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594939.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(1844..2263)
                     /locus_tag="IWK54_002897"
     CDS             complement(1844..2263)
                     /locus_tag="IWK54_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594940.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(2256..2489)
                     /locus_tag="IWK54_002898"
     CDS             complement(2256..2489)
                     /locus_tag="IWK54_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594941.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(2486..>3126)
                     /locus_tag="IWK54_002899"
     CDS             complement(2486..>3126)
                     /locus_tag="IWK54_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9594942.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT          870 a          703 c          435 g         1118 t
ORIGIN      
        1 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
       61 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
      121 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
      181 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
      241 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
      301 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
      361 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
      421 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
      481 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
      541 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
      601 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
      661 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
      721 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
      781 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
      841 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
      901 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
      961 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     1021 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     1081 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     1141 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     1201 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     1261 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     1321 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     1381 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     1441 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     1501 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     1561 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     1621 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     1681 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     1741 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     1801 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     1861 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     1921 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     1981 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     2041 ctgtgttatt gaccggtaat tgaccagtca attgatcact ttttgaccga taaattgacc
     2101 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     2161 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     2221 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     2281 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     2341 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     2401 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     2461 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     2521 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     2581 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     2641 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     2701 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     2761 tttcatgaac catctcatgc caatcagttg cttttcccct tcgtaactgc aattctagtc
     2821 gccaccatgt cgttatttcc ggcggaacaa ttttacgttt tctttcttgt tccagttttt
     2881 tattatacat ccgaatttgt cgttcactgg cacgactacc ccaatatgcc gtttctaact
     2941 taccagaacg cccataaatt ggtttaaaag acacaggatc aactacccga tattgtgtaa
     3001 taaactcatc aggtacatcg ataatgtcgc aagcaatatc cgcacgtgaa aagtgaggtt
     3061 cgataaacaa atcatgaata aaatttttca tggaactagc taagaattga ctaatcttat
     3121 tcggat
//
LOCUS       DACDTH010000146         3126 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDTH010000146 DACDTH010000000
VERSION     DACDTH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.151"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            103..1347
                     /locus_tag="IWK54_002900"
                     /pseudo
     CDS             103..1347
                     /locus_tag="IWK54_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="IWK54_002901"
     CDS             1360..1992
                     /locus_tag="IWK54_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9594943.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="IWK54_002902"
     CDS             2197..2424
                     /locus_tag="IWK54_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP9594944.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="IWK54_002903"
     CDS             2493..2759
                     /locus_tag="IWK54_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9594945.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="IWK54_002904"
     CDS             2749..3018
                     /locus_tag="IWK54_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9594946.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          942 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttt
//
LOCUS       DACDTH010000147         3031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDTH010000147 DACDTH010000000
VERSION     DACDTH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.152"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(64..1245)
                     /locus_tag="IWK54_002905"
     CDS             complement(64..1245)
                     /locus_tag="IWK54_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9594947.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            1497..3020
                     /locus_tag="IWK54_002906"
     CDS             1497..3020
                     /locus_tag="IWK54_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9594948.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT         1135 a          526 c          601 g          769 t
ORIGIN      
        1 ttaaaacaaa aacacaaaac gtcgttccta cgagctattc tcgtaagatc gacgttttgt
       61 gttttattta aaataatctt cttcagaaaa tgaagagttt tcatactctc ctttatcgcc
      121 ataaaattta ttgtagcgtt gtttgaattt acggaaaata aaagtaacga gtaatttgac
      181 taacgtatag ataggaatac caaaaataac gcccacaatt cccattagat cacccatgac
      241 aagtaaaacg attaagatcg taatcggatg gatctgtaaa cgatcaccca ttactcgagg
      301 aatcaccaag tcaccgtgga gaagttgtac ggcaaaccag acaatgacaa atttgacagc
      361 cataaaagta gagtcttgga atgcaatgat caaacctgga aagaaggcga taaacggccc
      421 aatatatgga atgatacata acaacccagc agaaatagct aaaacactag caaattctaa
      481 tccgatcagt aagaaacaaa cccagtagac tgctccaagg ataaatgaag caatgatttg
      541 cccctttaga aatgaaccca attgttgatt agcaatcttt gtcaaatttt taaaatctgt
      601 acgaaatgct ggtgggacaa tcgctaaaac tgactgataa aatttttgag gatctctcaa
      661 aaggaaaaaa gcgacaattg gaccagtaaa taaagtaata aaaatagctg taacagttga
      721 aaaaatgttg ctgatccgtt gtgcgccact ttcccaataa tttccaataa atccagctat
      781 atcagacgtc acgtcgtcaa tagaagcaaa aaattgatta aatgaatcgg caaaaggtgt
      841 tttctccaaa aattgtcgga gactctcttg aaaatcttga aacaaatctg gcaaactact
      901 gatcaatgtc tttgcctgtt cttcaagaaa aggaataagc catatgcttc caagccccaa
      961 taaaaataaa ataatcacat aaacgataat gactgacaag ttcctcgaaa ggtgcttgct
     1021 caaacgtttg atgactggtt ccaaaagata atacaagatc agtgcaaaca ataatggggg
     1081 caagatagta gcgataatga ttccgacagg cttgaatata aacgagatct gatggaaaaa
     1141 ataaatgatc agcgcaagta agataactat tcctaaaaca tagtaagagg tttttcctcc
     1201 taaaaagcgg atcaaacgat tcgttgtatc tgtttctttt ttcactcttt ttcctccttt
     1261 atctataact tcattataga caaaagcggg tataaaagga aaatgataga ggttgattgc
     1321 aaaaatatcg tcaatcattc atataaactt tctcatcaat taaaataaga aaacgcttga
     1381 cagttctttt aaccatatga taaattaaat cctgttgcta aaacgtaact attacggttt
     1441 gcgaaaagcg aatcggaaca ttcaaatgaa agaagatgag aagaggagtg tcataaatga
     1501 aacgattgat aggtatattg gccatgctgg ttttagcagg tatgttaact gcttgcgggg
     1561 caagtgggaa agccgaggac agtaaagaaa aattgagcgt gatgacgacc ttttatccca
     1621 tgtatgattt tacaaaagca atcgtcgggg atgaaggaga agtagagtta ttgattccag
     1681 cagggactga ttcccacgat tatgaacctt ctgctaaaga catggcaaag atccaagata
     1741 cagatatctt tgtttataat gatgaaaaca tggaaacatg ggtccccgca atccaaaaaa
     1801 cattacaaga aggcaatgtc catacgataa aagcgacaga aggtatgctt ttacttccgg
     1861 gaagtgaaga aggacatgat catgaccatg aacacgggga agaaggccat acacatgagc
     1921 tagacccaca tgtatggcta gctccaagtt tagcaatcaa gcaggtagca aacatacgtg
     1981 atcagctgat tgaagcttac ccagaaaaac aggaagtatg gacaaaaaat gcagcagcct
     2041 ataccgagaa acttcaagca ctccatcagc tttatcaaga aacctttaag caagcaaaac
     2101 aaagatcttt tgtcacgcaa catgctgcat tcaactatct agcgttagaa tatggattga
     2161 atcaagtatc gattgctggt ctttcttcta gtgaagaacc gtctgcggcg agaattgctg
     2221 aactgaaaca ctttgttaaa gaacatggaa tcaactatat ttattttgaa gaaaacgcca
     2281 aagatagtat tgccagaacg ttagcaaacg aagcaggtgt atctttagaa gtattgaatc
     2341 ctttggaagg attgacgaat gaacaaatag aaaatggaga aaactatctt tctatcatgg
     2401 aagcaaacct cgaagcgttg aaaaaaacga cagagacaga aaacccgcta gaagatactt
     2461 tgacccccaa aaaagaaaag agcgtctaca acggttattt tgaagacgag gatgtacaag
     2521 atcgctctct ttcagattgg gcaggaactt ggcaaacagt agatacgtat gtagaagatg
     2581 ggacttttga tcctgttttt gaatataaag aaaaattgaa tcaggataag acagcacaag
     2641 aatataaaga atattatacg aagggctatc aaacagatat tgcttcaatc aagatcagtg
     2701 agaaagagat ggcatttacg tttaaggatg gaacaacgaa acgttcaaat tatcgttatg
     2761 tagggaaaga aatcttgacc tacgaggcag gaaaccgtgg cgtaaggtat ctattcgaag
     2821 cagaagataa ggaaagtggc gcttttagat atattcaatt cagtgatcac acgattgcac
     2881 cggctgactc cgaacatttt catttatatg caggaaatga aagtcaagaa gtattgctga
     2941 aagaaatgga gaattggcca acattttatc cagatacaat ggatggacgt gaaattgcac
     3001 aggaaatgct tttgcactga taggcaaaaa a
//
LOCUS       DACDTH010000148         3031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDTH010000148 DACDTH010000000
VERSION     DACDTH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.153"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(251..865)
                     /locus_tag="IWK54_002907"
     CDS             complement(251..865)
                     /locus_tag="IWK54_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9594949.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1471..1848)
                     /locus_tag="IWK54_002908"
     CDS             complement(1471..1848)
                     /locus_tag="IWK54_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594950.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2148..3029)
                     /gene="galU"
                     /locus_tag="IWK54_002909"
     CDS             complement(2148..3029)
                     /gene="galU"
                     /locus_tag="IWK54_002909"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9594951.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          918 a          636 c          467 g         1010 t
ORIGIN      
        1 ttccactact ttttattttt ctgacactac atattcttat aactcatttc tcataactgt
       61 gaaacaactt ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc
      121 atcttaaaat aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt
      181 ctttttttca aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac
      241 atgtggataa ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa
      301 cttgctgcag caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat
      361 ttggcaaagt caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa
      421 attcaaataa tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc
      481 cagtaacaat gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg
      541 ccgggtttct tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct
      601 gatcaataat tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt
      661 caatcaatat acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac
      721 ttagtgtgcg gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc
      781 aagtgccagc ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat
      841 aatagtcaaa ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata
      901 gaaacagctg cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg
      961 tggtctttgc tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca
     1021 tagcaaataa aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc
     1081 ctagattagg tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt
     1141 cagggtgtat gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga
     1201 gccttttttt attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg
     1261 caagcaagaa aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa
     1321 aaggaatggc ttatcccgat acaacaagca ttcctttaaa aaagattctt ttatataagt
     1381 gaaaaaagct tttttccacc aattaaataa atctctcatc tcaatagttt atccattatt
     1441 ttatcttttt ttgcgtatac ctactaaata ttaccgtctt cttctcgttc ttctactttg
     1501 ttcgagagaa tctgtactag aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga
     1561 ctttcttttc atatatggga tataaatgat tagatacagt acagcaaaaa ataaagcaaa
     1621 tacgcaagct tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga
     1681 tgctgcatac cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat
     1741 aaatagacta ataagcaata gtggcaacac aaatatccaa ttgcttcctt ccatcagcca
     1801 cttgagtata tcaagcagcg actgtgcaac agcgactaat tttcccatag tgaacacaca
     1861 ccttttttta taattaaaaa attcgccttt ttattctact atgaatcttc tggcttggct
     1921 ttcatttctc ttatgtaatc attttctttt atacaactta aaaaaaaatt aaaccattag
     1981 cataatattt tctgaatcta ttcacgaaag tctttttcta tctttcgttt tccacgactc
     2041 aactgatcta gagtcaaaaa actaaaacga cagttgcgca aatgacgcac aagctgccgc
     2101 tttaataaca aatagccttt cattctcagc aggccactta tttccagtta actttctgtt
     2161 cttagctttt tactcaactg gataatatat gcacttaatg aagtcccaat ttccgaatgt
     2221 tgaagtccat attcaatatt tgtcttcata tatcctaact tatcgcctac gtcgtatctt
     2281 tttcctttaa actctctcgc atagaccgcc tctgtctggt taagagtctg aagtgcatcc
     2341 gttagctgta tctcgtttcc tgctcctggt tcttgagttt ctaatatctc aaatatttta
     2401 ggagtcagta gataacgtcc gataatggct aaattacttg gtgcttttcc catttcaggt
     2461 ttttctacaa acccttttac tctgcaacga cccttttcgc tatctgtttt aggatcaata
     2521 attccgtatt tagatgtttc attttctggt acacgcatga cggcaacagc ggaagcttgt
     2581 gtttcttcat aatccatcat caattgttga gtcaatggga tatcatcttt catcaaatca
     2641 tcccccagca tgacgacaaa cggttcattt cctataaatg cttttgcttg aagcacggca
     2701 tcccccagtc ctcttggatg agattgacgg atgaagtgaa gattgacttg agtcgtttct
     2761 tcaactaaag cgagtaattc tgttttttcc ttttcccgca aattgttttc caattcgata
     2821 tttgcatcaa agtgatcttc aatgggtctt ttttcttttc ccgttacgat caagatgtct
     2881 tcgattcccg aatccaatgc ttcttccaca ataaactgga tcgttggttt atccacgatt
     2941 ggaagcattt cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg
     3001 gcaggaatga ctgctttttt gaccttcact t
//
LOCUS       DACDTH010000149         3013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDTH010000149 DACDTH010000000
VERSION     DACDTH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.154"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            177..359
                     /locus_tag="IWK54_002910"
     CDS             177..359
                     /locus_tag="IWK54_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAP9594952.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="IWK54_002911"
     CDS             462..746
                     /locus_tag="IWK54_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594953.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="IWK54_002912"
     CDS             762..998
                     /locus_tag="IWK54_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594954.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="IWK54_002913"
     CDS             1020..1274
                     /locus_tag="IWK54_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594955.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="IWK54_002914"
     CDS             1356..1541
                     /locus_tag="IWK54_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP9594956.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="IWK54_002915"
     CDS             1608..2003
                     /locus_tag="IWK54_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9594957.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="IWK54_002916"
     CDS             1993..2370
                     /locus_tag="IWK54_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594958.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="IWK54_002917"
     CDS             2371..2766
                     /locus_tag="IWK54_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594959.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DACDTH010000150         2908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDTH010000150 DACDTH010000000
VERSION     DACDTH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.155"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            62..676
                     /locus_tag="IWK54_002918"
     CDS             62..676
                     /locus_tag="IWK54_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9594960.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(841..1665)
                     /locus_tag="IWK54_002919"
     CDS             complement(841..1665)
                     /locus_tag="IWK54_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594961.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(2303..2560)
                     /locus_tag="IWK54_002920"
     CDS             complement(2303..2560)
                     /locus_tag="IWK54_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9594962.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(2553..2822)
                     /locus_tag="IWK54_002921"
     CDS             complement(2553..2822)
                     /locus_tag="IWK54_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9594963.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT          958 a          506 c          388 g         1056 t
ORIGIN      
        1 atggcttcct cgtgtatgat tagggtgtat tttaaatagt tgcagaaagt aggtaaaagt
       61 tttgagagtt gcatacatac gtgtctcatc gatcgatcaa aatgaacaac gccagattga
      121 agaaatgaaa aaattcggtg cagagagaat tttcatcgag aaacaatctg gcgcaacgat
      181 tactcatcga cctgttttcc aagaagcatt agattacgtc cgagatcaag atatttttat
      241 tgtggaagct attgatcgac ttggaagaaa ttatgacgag atcatcgctt cagtcaatta
      301 cttgaagaag aaaaatgtga agctgatcat tactagttta ccgatcatgg ctgaagcaat
      361 tggcaatcct ctcctggata aattcattaa ggatctgatc atccaaattt tggccatgat
      421 cgctgaacaa gaacggactg aatcgaaacg tcgccaggca caaggaatta agatcgcaaa
      481 agcaaacggc gtctacaaag ggcggcccaa attatacagt gcagacgcaa aagatcccca
      541 acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa aatggtgttg cgattgctaa
      601 gattgctaaa gactacaatg ttacgagaca aaccgtgtat agaataaaaa aagacagcat
      661 ggtcaatgac aaatagttga tcatgctgct tttattatct tctcatcttt aatttcctac
      721 atataattac tatctataaa attaagtctt actcagaaca gataaagcat aaaattccaa
      781 caaaccattt tttacttaac taaataaaga gtctgttaat ataatcttca cttatttacg
      841 ttaatcccct actgtatttg ttggttcctt atctatattt tctacaaaat ccttattatc
      901 ttgtgattta gcttcgtata attctttgta tgtcgacaca ttggttatac taattcctat
      961 agctagttct tgagactcta cttttctctc tagctccttt gacaaagtga tttgagattt
     1021 atttggttcc gttggagtca attctataga ttcttcataa aaatctctat acataaagct
     1081 atcattaatt ttataaaaaa aattaaatgt ttttagcact gccatataac attcaaaaca
     1141 ttcttctata gttggtttac ttccttgagg agaatgaacc aatttattac gtttttctat
     1201 gaaattttta atttcctttc ttatttcttt cggaaactca atattaaagt accctttaaa
     1261 catgctccct aataatttgt ctacaggggg agattgtaaa ttctcaaata ttagagttaa
     1321 atcaggtttc tcttgttgat aaaattcctt tacccctatt tcacaggctg taattatcga
     1381 aataaaccta tctctagtat ttgatatatt tttacttctt ttaaggagta taagagaaaa
     1441 ggggatactt tcgtgtccat gcatgtaatt aactaattgt tgagcttcag aagttatatc
     1501 tctaattata tagttaacgg aattaggaag tgtctttaac tctaatgaaa tttctatcca
     1561 attactatct tctaatctgt atatgctcga agtataattt gattcgtcag caagcatcgc
     1621 tacatcccaa acactgatca taaaatcttt taaacgttca atcatttcag caatattgct
     1681 aaaatcagct aatttttctt tagaataaaa aaaacattta tatcagaatc aataaattct
     1741 atttttttta ttactatatc gtcatcatta tcttgaccat ttatgtttat tgttccctga
     1801 gtggggagta cttctttcaa attcaagtta aatttatatt ccattacttc tcctccaata
     1861 tataaaaatt acttaactgt atccaaattt aatatgtaaa taaatcatta atttttagct
     1921 aaattctatg agaagtttat ttttgttgtt tttgctaatt aactaaaact agtacaaaaa
     1981 atttaaaata gattattaaa cctattgctt attctattca gtcatatata taagcgtcat
     2041 cctttagtaa ctaacatccc acactacaat agtattataa catatctatt caacttcaaa
     2101 ttgttgaaat ttaagccatt tatatttact tttcctcaac tttagaaatg attttactaa
     2161 ccttatatcc gtaatacgcg taacaaacac attcattaat aacaatcaac actaagctat
     2221 attagctttt taaatgacta gggtaaataa aataaggatc aaaaactttt aagtttctga
     2281 ccctttcctt acttccgatt ggttaatagt gatcttttgc agaataaata aatatcgttt
     2341 cattttcaac tctatatatc agtctatgtt catctgtaat tcttctggac cattttccag
     2401 ataaatcatg ctttaatggc tcaggttttc ctaatccagc aaagggggaa cgatcgatat
     2461 cttttattaa cttgttaatc ttttttatat tgcttttgtt tccttgctca tgccaataaa
     2521 gataatcatc ccaagcatca tcagaccaag ccttaatcat cagattcaac ctcgattaag
     2581 tcatgtgttt taaatgcacc tttggagaat tgttcatctc ctcgacgaat tttttccatg
     2641 acgtaattat tagaaagtgt tctcaacgtt tcttgcatag aatcataatc tctttttgat
     2701 aatacaacaa ctgtatcttc tacatcttta cttgttacaa taagtgtttc agcatcctca
     2761 ttaacttgtt tcatataact acgtaaattt tggcggaaat ttgaataagc tactgcttcc
     2821 attcctttca ccccttattt atttctataa aacaattgta cattatattg tacaattaag
     2881 caaatgatta atattctaac ttcttata
//
LOCUS       DACDTH010000151         2826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDTH010000151 DACDTH010000000
VERSION     DACDTH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.156"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            798..1064
                     /locus_tag="IWK54_002922"
     CDS             798..1064
                     /locus_tag="IWK54_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594964.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1122..1634
                     /locus_tag="IWK54_002923"
     CDS             1122..1634
                     /locus_tag="IWK54_002923"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9594965.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="IWK54_002924"
     CDS             1631..2668
                     /locus_tag="IWK54_002924"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9594966.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1022 a          496 c          564 g          744 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacac
//
LOCUS       DACDTH010000152         2723 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDTH010000152 DACDTH010000000
VERSION     DACDTH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.158"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IWK54_002925"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IWK54_002925"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9594967.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1067 a          420 c          558 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaa
//
LOCUS       DACDTH010000153         2640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDTH010000153 DACDTH010000000
VERSION     DACDTH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.159"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..153)
                     /locus_tag="IWK54_002926"
     CDS             complement(<1..153)
                     /locus_tag="IWK54_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9594968.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="IWK54_002927"
     CDS             complement(475..696)
                     /locus_tag="IWK54_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594969.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="IWK54_002928"
     CDS             complement(794..1039)
                     /locus_tag="IWK54_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594970.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="IWK54_002929"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="IWK54_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2263..>2640)
                     /locus_tag="IWK54_002930"
     CDS             complement(2263..>2640)
                     /locus_tag="IWK54_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP9594971.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          889 a          465 c          362 g          924 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
     2101 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     2161 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     2221 ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga
     2281 ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct ccataataac
     2341 agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt cctctgaaat
     2401 caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct ttaaaattcc
     2461 cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac tccttaaatt
     2521 gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg aaaagataga
     2581 attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa attctattgg
//
LOCUS       DACDTH010000154         2608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDTH010000154 DACDTH010000000
VERSION     DACDTH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.160"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            74..403
                     /locus_tag="IWK54_002931"
     CDS             74..403
                     /locus_tag="IWK54_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9594972.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            393..680
                     /locus_tag="IWK54_002932"
     CDS             393..680
                     /locus_tag="IWK54_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9594973.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            964..1821
                     /locus_tag="IWK54_002933"
     CDS             964..1821
                     /locus_tag="IWK54_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9594974.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1822..2370
                     /locus_tag="IWK54_002934"
     CDS             1822..2370
                     /locus_tag="IWK54_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594975.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
BASE COUNT          979 a          338 c          519 g          772 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
      421 atgtcattaa gtatttaaaa aagctaaaag agaagccatt gaaagaaaaa tttttgaatt
      481 taatttatga tgaaattgcg attgatccat ataagggtga acaaaaaact ggggatttat
      541 caggtatttg gtctagtggg ttcaaatatg cgggcacaac atatagagta gcttacgaga
      601 ttatagaaaa caaggttatt ccagtattgt tatgtggaac gcatgaaaac ttttatgaac
      661 agttgaaaaa attaatctag tgatttgttg tcgttcttaa gaaggacggc ttctttttat
      721 cgaaaatctg attcgtctac atgactaaag tcgcgagtat tctctgcttc tcgataaatt
      781 aaataacgtc attactttga ggaaagctat aaatcaatat atttcagctt ttccttattt
      841 atgtactacc ttttacttat taattataag gtagtacata tatatctttt tttggtagta
      901 actaaaaata aatatgttat aatagtatca aatgaatatt gctaaaagaa aagaggtagg
      961 aacgtggtag cgaagaaata tgtaatcggt aattttaaag gtggcgtagg taaaagtacg
     1021 tgcgcgcaaa tgttagggtt tgaatcagca gttaatgaag gaaaaagaac cttgataatt
     1081 gacttggata tgcaaggaaa tacttctgat gtgatgaact taacgcatat gaacttcact
     1141 aacgaagaag gaggcggaga aggtgaaccg attgtttacg aaaatactgt gactgacgtg
     1201 ttgataaaca atcttccacc taaagaggct gtctataaag taatcaataa cctctatata
     1261 cttccagcag acatgtcctt tgaaatgtat gatgattgga tcaaagacca atataatgat
     1321 tcattatccc aattcaaata tatgaaagaa aagctagaac cattatttga tgagtttgac
     1381 gtcatctatt tagatgtccc accttctatt tctgtatata gtaaatccgc gatgtacatt
     1441 gcggattggg caatcgtcgt cttacagacc caagtaaaat cgatgagaaa tgcgatgcaa
     1501 tatcttgagt atatggactt ttttgtgaaa gaatttgata caaatttata tgttgctggt
     1561 gttattcctt tcatgttgga aagcggtgac gcagtagatc aggaaatgta taaacaagcc
     1621 caagagattt atggggaaca tttgattaaa aatgttgtac tcaaaaatgc gcgcttaaaa
     1681 cgatacgacg gatcaggtat tacgatggag aagacaaaga aaggtctctt aaaacaatgg
     1741 gatcgcaaaa gccacgaatt atttatcagt attttgaaag aattggaaga acacgagaat
     1801 tggtatgcgg aggtggagta attggctgac atcaaaggac ttgatatttt tgggaacaac
     1861 aaaggactcg ccaaaataaa aaaagaagca gaagcaaaga aaaagcagca agcaattaca
     1921 ccatctgaaa ataagcaatt agaagtgcac caagttcaag aaataaaaac ttcctccaat
     1981 gttgaaagac agagtagagg aagaacaaaa aaagttggcg caccaatcag aaagtttgat
     2041 aaggtttatt ccaccaagca tcctttaaaa ctgtcgactt tgttaaatgc gacttccaga
     2101 gttttagtag aaaagtatga gactaatatg acaagagatg aattgcttag aaaagcttta
     2161 gatgagtata ttaaacagaa tcttacgcaa gaagataagc aagatttgtt taatgacgtg
     2221 gtgaaagaac tagacttgtt cagagaaaaa tatccaattg ttccagaatt agatgatcgt
     2281 ggagaaataa taagaactac cagtcagata aaacaagata ccgaagactc actgcgcaaa
     2341 gggtggggaa tgaataataa agttagataa taatcaaaga agaactaagt aactaccttt
     2401 aaacggtagt tacttagttc ttctttgatt tgcatgaaat agaggggatt tatttttctg
     2461 gttacgtgat taaaaaatcc aaaaaatgag cataggaacg gttatgattt tagggtgtgt
     2521 tttaatgaga tattgtgatt tcattaacaa gtgtatgata atctgttgtt gagttagtaa
     2581 ataagtaata gagctaactc taaaataa
//
LOCUS       DACDTH010000155         2590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDTH010000155 DACDTH010000000
VERSION     DACDTH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.161"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1031..1549)
                     /locus_tag="IWK54_002935"
     CDS             complement(1031..1549)
                     /locus_tag="IWK54_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP9594976.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          842 a          467 c          368 g          913 t
ORIGIN      
        1 ttttctatat agttggatta agataacgac tatttatttt atcatatttt tactataccc
       61 tatactttta cttggtatat ttatgaataa tcgcaaattt acccctatac tactagtttg
      121 taagttagat taataaaaat actaaagtta tttgttcttt tattaaatac acctacaact
      181 tataaattga tggtatttag ataacacgaa agaataaaaa taacagctga tagtaaagtt
      241 tgttgacgat tgctactcaa tcgaaagtga gttagtgcgt atgaacttgc ttctgaaaac
      301 ttatgaaatg aaagaccttt tacatcttgc agaaatcggt actgggatta aactaagcaa
      361 taaaaactaa tgttcccctc attctttgaa cttctactat ttgatttatt tattctgata
      421 aatgacgatt tcaccttcag gatcttcaaa tcccgtatag aatttattta agatctcttc
      481 agtatcagta ataggatagg cgataggcca catacccttt gatggttaga ataaactgct
      541 caaaatctgt atcgtatgtt ggctttagca tattaacaaa ggctatctcc aaattataaa
      601 ccataagcgt tgaatcgttc ttaacaattt caattcttcc taacccaaag tcagaagata
      661 taattaccgg acttgcctta cccttcatgt gggttgttga gattccatat ttaaagggaa
      721 ttgtcggtac tatcggcggt ctgaaaaaca tcccaaaaat aaaaaaatct ttcccctgct
      781 agtctcctaa gtccctttgt agcggatatg gcaccataat ctcattttat cgaagtgagt
      841 catggtttat ctgttggcca accaaaattg atacaatcta acgatgcacc ctctacggca
      901 ttctagggga gtgcacttgc attcacccaa taaaaaccgg cagttagcaa agaacttatg
      961 tagtcgtatg ttcttactgt taacatacaa gaggtctcct ttccacaatt gcttagcagt
     1021 aaacgggaat ttacaaagct aatctcttcc attttcttat ctttgttcta aaagtcccaa
     1081 atggagcaac tgtatttaca tgaataaatt tatatacttc ccatacagcc gttttggttg
     1141 cctcgtctgc ccattttctt ttgtgtgcag tgaatagatc ttcatcactc attgaatcaa
     1201 tcatcatgtt aatatcatca atattacgat tgagaatttc ctctaattct ttcaatggta
     1261 gatgtgcata ggtatttgta aaccacttat ataactctcc taactgattc catttaaatt
     1321 gatcagaagg cgttttaaca gataatccat ttctttcatc attttcccat tttagaatta
     1381 aattcgtcca accaacttga tatgctaaat tttcagcagg tgttctatcc acttcctcaa
     1441 ctcttttatc cttcaattct tcaggaatag tttcaaattc taaaatatat ttagtataag
     1501 tcctatttat ttcttttttt aattcttctt tatttttata tgttctcatt atattacctc
     1561 cataaattcc taattgtctc ctttaattca tctataattt catccacgat attttaatcc
     1621 gttctacaat gcatatctga tatgcattga ctgtctaact catatgtgaa tgatttataa
     1681 aatatcccaa cgtctcatct aaagaaatgt tgatcgtcag caaggagcta aaaaatacga
     1741 atatatttct tttgtagtaa aatcggactg gtttgcatat cattttcttc gtaaatatca
     1801 aaatcatgtt aactactatc aattactaat aaaaactacc actttaagcg ttaagaactg
     1861 ttaagtttag ttaagtgagg atagttcaca aaatccattg ttaaacttca cgaaagtggt
     1921 aaaactcaaa atgctttata taggattaca gcatcgctct ttcttcgatt gcgcgttggg
     1981 taaaaaatat tatgaagtaa aaattgacga tgacacgttc cttacggctc atcagatgaa
     2041 acagctacaa aaacgaaatg atcaactcga agaaaattta attctaaaaa agcgattgcc
     2101 atattcacgc ctcgctcgac gaactattaa aggtcaagat ttacctgaag atcatccttt
     2161 ttaggaaatt gaagcttttt atgttgcaca atgcatttat aatactacaa tgatgttttc
     2221 accagatatt cttctagggt gcggtgtaat gaaacagata cacttgcaaa agaaaattca
     2281 ccgcgaattt actaaaatta tgaataatta tgttaatctt ccgaaatttt tttccaaaat
     2341 taagggatca agctggtgtt atagattgtt taaggttggc aaaaaaataa cttcttttta
     2401 aacaattata gaaaaccgat actctgttaa gttggttgac acggtgtcga tttttatgac
     2461 tttcttattc tgaaacttca ctaacggaca gtacctaaaa agtctatagt atggacgttt
     2521 acgagtacta ttttatcaaa aatgctgctc atttcaactt atatgtttta gtgaactacc
     2581 cccacttagc
//
LOCUS       DACDTH010000156         2567 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDTH010000156 DACDTH010000000
VERSION     DACDTH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.162"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            75..470
                     /locus_tag="IWK54_002936"
                     /pseudo
     CDS             75..470
                     /locus_tag="IWK54_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            790..1104
                     /locus_tag="IWK54_002937"
     CDS             790..1104
                     /locus_tag="IWK54_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9594977.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="IWK54_002938"
     CDS             1208..2386
                     /locus_tag="IWK54_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9594978.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
BASE COUNT          841 a          377 c          525 g          824 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtatt
//
LOCUS       DACDTH010000157         2548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDTH010000157 DACDTH010000000
VERSION     DACDTH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.163"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1131)
                     /locus_tag="IWK54_002939"
     CDS             complement(<1..1131)
                     /locus_tag="IWK54_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9594979.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDD"
     gene            complement(1261..>2548)
                     /locus_tag="IWK54_002940"
     CDS             complement(1261..>2548)
                     /locus_tag="IWK54_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9594980.1"
                     /translation="LISLSIKDATDRASKETFANITNSFSMEINRQVNPGTPRGGGNV
                     KGEDIKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTG
                     VNDSTKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDA
                     DNEKGADETVEVEIKGLFDGHNSGGVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQ
                     DATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFG
                     SLIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYF
                     LAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKF
                     MIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT          773 a          494 c          375 g          906 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgcgcattc tcattagaga acactccctt
       61 ttttgtgact tttctgaagc tagagtgaat actttcaact gcattagtag tgtacatgat
      121 ttttctaatg gcgctgccat aatcaaagag ttgttcaaca tgagaaaagt ttcgtttgca
      181 tacatctact gcaccagaat aatgagacca ccgattttga aaactgtcga aggcagcgtg
      241 tgcagcgttt aaagaagaag cactgtagaa ttttttcatg tctcgacaga cttccttata
      301 atccttactt gggaggtatc gtaacgcatt tcgaacaaga tgaacaatac agcgctgcac
      361 aatgacagaa ggaaaaattg cttttgcgcc ttctttgaga ccggaaacac cgtccatcga
      421 aatgaaaaaa acatcttcta caccacctac tttcagttca tcaaatactt gcatccagcg
      481 atttttagat tctgtttgat tgagccatag gcctaaaatc tccttgtttc ctttaagatc
      541 atagccaaga atggtgtaca cagcatattc tttagcttca tagttttcgc gtaaagtgac
      601 gtacatacaa tcaacgaata gaaatgcata acacttggct aatggccgag cttgccattc
      661 ttccaattca ggaaggacgg catccgtgat atcagaaatc atttcgtggg aaatatcaaa
      721 gccgtaaata tcttcaacgg ttgcggcgat atcgcattga ctcattcctc gtgcatacat
      781 ggaaagaacc ttaccttcga tgtcggagac atctcgtttt ctattaggaa tcaactcagg
      841 ttcaaaagaa gcctcccggt ctctgggaac atcaatgttt actttaccaa agctcgtttt
      901 aagcgtctta ttgccataac cgtttcgtcg attgtcatgt ttctttggct ctttagaatg
      961 ggcgtcatac cctaaatggt tattcaattc tccttgaagc attttttcaa ataagggacc
     1021 aaacacatct tttaaaacat cttgcatatc ctctacagat tcgggttgat aagtattcaa
     1081 aatggattca gctaacttct cagcatcagg atttcttttc tttctagcca tcgtgtaatc
     1141 acctcttgat cttattttag aaaaataaaa accgtgaagc aaccacccgc ctaggattga
     1201 tggttactta cacggtttat attacactct catttttggt gctaaaaagc accgaaaatt
     1261 ttaattattg tcaattaata attcttttgg attccttctt aatatattga atgaagaaat
     1321 aatcaatgat acaagaagta ctaaactcat aaatataact acataaatca tgaatttagg
     1381 taatacattg atatctaaac ttgataatgt cttgttaaat ccttcagctt ctgccccacc
     1441 acctaattgg ctagatgcag attgtttagc tatttgttta gctatatcac cagtaacctt
     1501 attcaatata ttattcccaa gcttgtcagc tgtatattga gctaagaaat aggaaccaac
     1561 gaatgcgggg attgatataa ataccatctc aattaggaat tgaccaaaaa tttctaattt
     1621 tgatatacct aatgatagta atactgctat ttctttcttt ctagcattca tccataagaa
     1681 taataataat gaaactacaa ctcctgcaaa tattaatgag ccaaaaaata atttatttga
     1741 aattgagtaa atacctgata tagattgttg taatgcaggg taatttgatg aacttttaat
     1801 caaattatat tctctccaat ttatatctaa tttcccaaga tcttttatta cactatcaag
     1861 attcttatca ccttttacaa agaatgttgc atcttggtat acagctgtat cttcggtatt
     1921 accataaact ttagcagctg tatgaatatc agtgattaat gtattttcgt atagttcttg
     1981 tgctgcactt actccaccgc tattatgccc atcgaataga cctttaattt ctacttctac
     2041 tgtttcatct gcacctttct cattatcagc atcaaataaa ttagatttta tttttatctt
     2101 atctccaact ttaaggttat ttttttcagc taaatcttta tgcattaaga ttttattttt
     2161 atcttcattt tctaaatgct ttccttctac taatttatat gcttctgata caaattttgt
     2221 ttcttttgtt gagtcgttaa ccccagttaa cataactgtt cttttaaaat tctttgctct
     2281 ttcaggtgat tgattcgcaa gagtctcttg agtttcaata atatcataat caactaaatc
     2341 tgcaacactg tttattcttt ttacatagga gtctatacta tctgtttgag atatcttttt
     2401 aatatcttca ccttttacat ttcccccacc tctaggtgtt cctggattta cttgtctatt
     2461 tatctccata gaaaaactat ttgttatatt agcaaatgtt tcttttgaag ctctgtccgt
     2521 agcatcttta atagataaac taatgaga
//
LOCUS       DACDTH010000158         2453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDTH010000158 DACDTH010000000
VERSION     DACDTH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.164"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IWK54_002941"
     CDS             132..344
                     /locus_tag="IWK54_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594981.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="IWK54_002942"
     CDS             506..1024
                     /locus_tag="IWK54_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP9594982.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="IWK54_002943"
     CDS             1031..1543
                     /locus_tag="IWK54_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594983.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1814..2437
                     /locus_tag="IWK54_002944"
     CDS             1814..2437
                     /locus_tag="IWK54_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9594984.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          943 a          310 c          408 g          792 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
     2281 gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg aaattgttga
     2341 tggtctgaag aaaaaagaaa ctatttctaa aatagcaaag aacgcaggag ttacaagaaa
     2401 aaccgtttat cgagtaaaaa gagatttagg aatgtaatat gttccagtta gaa
//
LOCUS       DACDTH010000159         2415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDTH010000159 DACDTH010000000
VERSION     DACDTH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.165"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            144..494
                     /locus_tag="IWK54_002945"
     CDS             144..494
                     /locus_tag="IWK54_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9594985.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="IWK54_002946"
     CDS             908..1726
                     /locus_tag="IWK54_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9594986.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="IWK54_002947"
     CDS             complement(1799..2158)
                     /locus_tag="IWK54_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9594987.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          799 a          351 c          450 g          815 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgataaattt tctctatttt ctacaatatt taaaacgctt
     2401 ccttagtcta ttata
//
LOCUS       DACDTH010000160         2407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDTH010000160 DACDTH010000000
VERSION     DACDTH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.166"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IWK54_002948"
     CDS             5..1150
                     /locus_tag="IWK54_002948"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9594988.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IWK54_002949"
     CDS             1321..1779
                     /locus_tag="IWK54_002949"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9594989.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="IWK54_002950"
     CDS             1938..>2407
                     /locus_tag="IWK54_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9594990.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DACDTH010000161         2390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDTH010000161 DACDTH010000000
VERSION     DACDTH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.167"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            197..2059
                     /locus_tag="IWK54_002951"
     CDS             197..2059
                     /locus_tag="IWK54_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9594991.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2390
                     /locus_tag="IWK54_002952"
     CDS             2059..>2390
                     /locus_tag="IWK54_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9594992.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          860 a          353 c          465 g          712 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACDTH010000162         2298 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDTH010000162 DACDTH010000000
VERSION     DACDTH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.168"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(69..251)
                     /locus_tag="IWK54_002953"
                     /pseudo
     CDS             complement(69..251)
                     /locus_tag="IWK54_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            238..435
                     /locus_tag="IWK54_002954"
     CDS             238..435
                     /locus_tag="IWK54_002954"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594993.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(478..1053)
                     /locus_tag="IWK54_002955"
     CDS             complement(478..1053)
                     /locus_tag="IWK54_002955"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9594994.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1208..1734)
                     /locus_tag="IWK54_002956"
                     /pseudo
     CDS             complement(1208..1734)
                     /locus_tag="IWK54_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          693 a          427 c          375 g          803 t
ORIGIN      
        1 tttaccactc caaacaggga gcgtcaataa ttttgtgtaa ataactcgtc cttctgcaaa
       61 ataatgggtt actcagtaaa cattgaagct aatgtatccg tcacttgttg gaagccttta
      121 tggcttctat ttaagaattt ttgattgtat gtatcaaaga tggatactag gaaacgctct
      181 agtgattctt cattttgaaa ctgctctttt ctgcggctgt atttcttgat ttgtttattg
      241 aaagattcga ttgtagaacc agaaggaatg gagaacactt ttgaacttta caacaaaact
      301 actgttgata aattaattcc aattttagtt ccaactgatt taaatggtca tgatgtttta
      361 cttcgtttta acggattttt aggtgaggat tatagatacc taaaaatttc tgatcctcaa
      421 cctttaacag aataataaaa aacgtgtaat caattatttg attacacgtt ttttatttca
      481 atcttcgttg tagtagtggt tgcgacatac cgaaaggtaa tcttcattac ctcctactac
      541 aatctgttca cctgaataaa ctggaatacc atcaacaata cgagcattca ttacagcctt
      601 tttattgcaa aaatggcaaa tagttttaat ttcctcaatt ttatcagcaa atgtcaataa
      661 tgcttcggat cccttgaaga gattattttg aaaatcattc ttcaatccat agcaaattac
      721 tggtacttcc tcattcatta cgataagtga tagatcaata atattctctc tacttaaaaa
      781 ttgagactca tcgaccaaaa tacaatcaac ttttccgtac tgatcaataa tctccactaa
      841 ttctccgtac acatccattt cagaagtaat tgcaatagct ggtttcgaaa cacctatacg
      901 actctcaact tttccaaatc ctgatcgagt atctattgcg cttgtcatta tgataactct
      961 cttcccttgt tcttcataat tatgagccac tttcagtagc tcaatactct ttccactatt
     1021 cattgttcca tatctgtaaa ataattgtgc cattaaattt attcccttct tttacaattc
     1081 aattattcct taattccatc tgcttttatg cagctgctcg tttattcctt gatatgtcta
     1141 taacatctac tatccctaaa accaatttat aagccattag agatggtatt cctgaacatc
     1201 tctcatattg ttttttgagt ggtatatcat ctgaaaaact gaaggaatca tgaaaatagc
     1261 gacatactta tcatttagag ttctaacttt aaatgcattt acttcttcag tcagcacttt
     1321 agtcatgttg gacatggttt ggggcgtgta gtgatgccca tacattttct caatcaagtc
     1381 tgcgatttcc gacatggtaa taccttttcg gaagaggtga atgacggtct cctctaatgt
     1441 gtcattcgtc cgtctataag caggaacagt ctgttgcttg aactcgccat tgcggtcgcg
     1501 cggaatctgg agatgaagtt ccccatactc ggtcttgacc gtacggtcat aggagccgtt
     1561 acgagaatta cccgtgttaa aaccaatgcg atcgtacttt tcgtaatcga ggaaagccgt
     1621 taactccgtt ttgagaagcg tattgacagc tttttccaaa tggacacgga ataatccatt
     1681 caaatcgcct ttgttcgcta gagtctttag gatttctgta gtaaaatcgt tcatatggaa
     1741 gtcctctttt ctgtgaatgt gttgtggtaa ctctattcta cagaagggac ttcctttttt
     1801 ctatttacac aaaatatttt acactctcaa caaaagtatc ttcgattaat acttgctcaa
     1861 gttatgtgca gctacgaaaa agtaggtacc gaggtacttt ctttttcgca gctttttttg
     1921 gacttatgag aagtacattc gctggaagaa tacgatataa aacgagcttt ttcactaaag
     1981 atattaatag agtgaaagaa acaagctttt gttgtcagtt ttttcacttt tctaaaaatg
     2041 aaaataaaaa attctggaag tggagaagaa ttttgcttgc tatactttgt cttgtagaaa
     2101 agaggtggac aaaataatgc aagctcaaat aagcgatttt ccataacctt ggtaggaatg
     2161 acaaattaaa taggaaaggg aagtaataat atgaataaaa aaaagttatc tctattcggt
     2221 ttttttgccc tgacagcttc catggttttg actgtttatg agtatccgac ctttgcaaca
     2281 tcacaattac atttggtt
//
LOCUS       DACDTH010000163         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDTH010000163 DACDTH010000000
VERSION     DACDTH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.169"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IWK54_002957"
     CDS             <1..378
                     /locus_tag="IWK54_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9594995.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IWK54_002958"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IWK54_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9594996.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..2026
                     /locus_tag="IWK54_002959"
     CDS             1859..2026
                     /locus_tag="IWK54_002959"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9594997.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            2080..>2232
                     /locus_tag="IWK54_002960"
     CDS             2080..>2232
                     /locus_tag="IWK54_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9594998.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT          628 a          371 c          483 g          750 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttactggttc tgttgcaaag ttttaaatct actatcaaat
     2041 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt taaaggaaag
     2101 caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc
     2161 tatcgtgaag ttcgagaaat cttatatgat cgtggcatta acgtttctca tacgacgatt
     2221 tatcgttggg tg
//
LOCUS       DACDTH010000164         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDTH010000164 DACDTH010000000
VERSION     DACDTH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.170"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IWK54_002961"
     CDS             <1..544
                     /locus_tag="IWK54_002961"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9594999.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="IWK54_002962"
     CDS             complement(900..1472)
                     /locus_tag="IWK54_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9595000.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="IWK54_002963"
     CDS             complement(1488..2093)
                     /locus_tag="IWK54_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP9595001.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          700 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ct
//
LOCUS       DACDTH010000165         2217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDTH010000165 DACDTH010000000
VERSION     DACDTH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.171"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..2155
                     /gene="acm"
                     /locus_tag="IWK54_002964"
     CDS             <1..2155
                     /gene="acm"
                     /locus_tag="IWK54_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9595002.1"
                     /translation="RNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTNI
                     TDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTV
                     RKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTK
                     PASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPST
                     FEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVS
                     YKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIF
                     KYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEA
                     PNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFK
                     LFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVP
                     SGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEV
                     SEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPG
                     SHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKS
                     ILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          873 a          352 c          421 g          571 t
ORIGIN      
        1 tagaaatatt attttatcaa tgttatttat tattactaat ataacatcac tgattcctgt
       61 tcatgtctat gcggatgcag gcagagatat cagcagtaat gtcacttcgt tgaccgtgga
      121 cccaacgaat attacagatg gaggaaacat aaaagtaaaa ttctcgtttg acgaaaaaaa
      181 acaaaatatc caaccaggtg attatctttg gatcaattgg ccaagtgaag gaaatattcg
      241 cggtgaagga ttccaaaaag aaatcccatt aatgattgaa aataaaaatg ttggaacttt
      301 aacggtcaga aaagattctg cgcaggttgt gtttaatgaa aatattaaaa acctagactc
      361 agtagaaggt tgggggcaat ttgagataca ggccagaaac gtaaccgata ccggtgaaga
      421 aaatacggga ccattcaccg tgacgagcgg tgataaaaca gctactgtaa atgtgacaaa
      481 gccagcttct ggttcttctt ctagcgtttt ttattataaa acaggagaca tgctacctga
      541 agatactaag cacattcgat ggtttttgaa tattaacaat aacggcactt atgttgaaca
      601 accagttaaa atcagtgatg aaatccaaag tggacaaaga ttagatccaa gtacgtttga
      661 aattaatcaa atacacttag gtgaacagaa ggtttataga ggagaagagg gaatccaaca
      721 atttttacaa gatttcccca gcgctacatt taatttttca gtaactgata attacattga
      781 aataactata ccaaaaaact ttgtcaatct taggaaaata atggtgagtt ataagacgat
      841 catagaaaac ccagaacaaa taaattttga gaatcattcg gaagcttggt ttaaagaatt
      901 taataaacct gctgttgatg gagaaagttt caatcataca gttaaaaata tttcagcttc
      961 tggtggagtc aacggtacag ttagaggcga attgaagatt ttcaaatata tcaatgacac
     1021 agagattggt atcccaaatg tgactttcga attaagacga gcagatgaac agcctattca
     1081 aggacagtct agtatcctgt tgacttcgaa tgaacaggga gaagctagta tcaaaggcct
     1141 gcaagtagga gactatgttg taaaagaaaa agaagcaccc aattggattg attttgatcc
     1201 actaagttca aatgaactca aattctccat aaacgaaaat gatacagagg gagtcagttt
     1261 acctatttat aacaagaaaa aggttacgaa catcacagca actaaaattt ggaacggtgg
     1321 aaccactcca agaccgagta tttattttaa attattcaga gcaagtacaa acaattggga
     1381 accagttcct gatgcagaaa caaaacgatt agacaatgga ataacttcag tgacttggga
     1441 agatatccaa cagtatgatg atagtggaaa cgaatatacg tttaaagtgc aagaagtaga
     1501 tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa attgaaaacg ggctagtcgt
     1561 tacaaatgag aataaagaag tcatttcagt ctcaggacaa aaaacatggg aagacaatga
     1621 gaaccaagac ggcaaacgac cgacgacgat cacagtaaat ctattagccg acggtcaacc
     1681 gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca caaatctgcc
     1741 aaaatacaga gacgggcaag aaatcatcta tactgtaacg gaagacaacg tgcccgaata
     1801 cagtacgacg attgacgggt ataacatcaa aaacagttat acaccggaaa aaacaaatat
     1861 atctatacca ggtagccata taacaccgtg gaaaaatttt ccagagaaga cagagagaga
     1921 gaaaattaga aatctattct ctcaaaaatt gcaaatattt gctcatacag gagaatccag
     1981 cgaacaaaga agcaattata ttagtaagag agctgtacct aaacagataa caaataaatc
     2041 aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa tcattggttt
     2101 attgatagta actatctgtg ctgggatatt cttacaaaaa tataaaaaag tttagttaga
     2161 atataatatt ttttgttaaa tatgactgaa atcaaaagaa taaaattccg atttata
//
LOCUS       DACDTH010000166         2177 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDTH010000166 DACDTH010000000
VERSION     DACDTH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.172"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(234..494)
                     /locus_tag="IWK54_002965"
     CDS             complement(234..494)
                     /locus_tag="IWK54_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595003.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            complement(860..1345)
                     /gene="ssb"
                     /locus_tag="IWK54_002966"
     CDS             complement(860..1345)
                     /gene="ssb"
                     /locus_tag="IWK54_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9595004.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1348..2163)
                     /locus_tag="IWK54_002967"
     CDS             complement(1348..2163)
                     /locus_tag="IWK54_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9595005.1"
                     /translation="MMEKLLECIVESPYHDDFVLKGGFLIGSKYGLENRSTVDIDTTY
                     RNSKLTKEKLETVFSELTAAPTKDGIRFELKGLTETREADYYPGFQAKFIAFLENARV
                     PFKLDITSGDSIIPAALIYEHKLMFEDKTIHIPAYPTEQILAEKLHATFSFGIDNSRM
                     KDFYDLYMIPKLERIDEINLYKSVQGTFKERNNQSVFKNLYNQEMPQLMKNEGLREKW
                     KRYQQENYFAKDISFESTLTSVDSLMQSIVEQEEVERKQNFKRSQQMNQEMER"
BASE COUNT          630 a          420 c          376 g          751 t
ORIGIN      
        1 aaccttttgg tgagaggaag ttttgtctat tttgtcttgc tttgcaagac accatatctt
       61 agtgaacgtg cagcgttcac ttcctttctt ctcgaacggc ggtggcggtt ggagtgagag
      121 aaaggttttt caattgagaa ggcacttgtg tcttcctaaa aaggtctttt cctgtttcta
      181 tgtaaaacaa aaagagctgc aatcctcatt taggactaca actcttcttt tatttatatg
      241 cccatcgatc gcgtaaaatg atcgggattt ctaacataca tgctagcaat aactagctga
      301 tttcgtttat tgaagtgacc tgtgacggca atattgtatt ttccatcatc cataattagc
      361 aatttatcga taatttcaat atctgcaact acacaattaa tcgattcatg aatcgtttgt
      421 aaagtaaagc gaaccaataa cggttgcgtt tttattactc ggattttatc aagcaaacca
      481 acatatttgt tcatcacaat cacctcgcta aaattatagc aaaaatccaa tcatttggat
      541 atttcaatta tgctataata gtcttgctgt ctgctacccc ggcctaatct ggcaagggga
      601 ggaggttata ggtttgaaaa ttctttttga ttttgttttt gcaatcatgg cagaagtagc
      661 cgcgcactat atctgcaaat ggcttgattg taaatttgcc aagaaagaca gtaagcctac
      721 ccgttaattc ttataataga aacgtaaaag aaagccacta gcccgccagc tagtggtttt
      781 tggttataga ttgaaaattc tttttgttgt ctaaattata acataaaccg tagcaaatgc
      841 aaccaataga aaatgctact tagaacggta gatcatcatc gctaatatcg attgattgtc
      901 caccaaatgg attctgtgaa gcgttcaatc cattgttgtt aggcgtcgct gtatcccgtt
      961 gttcaaaggt ggtagggctt ggactgctgg acgattgatc agcgtgcata cggctttctg
     1021 tcgctgcttt actttctaat agttggaaat tatcggccac aacttcagtc acatagacac
     1081 gctgtccttg ctggttgtca taagtccgcg tttgaatccg tccgacgacg ccgatcaagc
     1141 tgcctttttt agctaatgtc gctatcgttt cagctggttt gcgccaaatc acacaattga
     1201 taaagtcagc ttctcgattg ccactttgat tagtaaaatt acggttaacg gctaaggtaa
     1261 aagtggtcac tgcagtgcca cttgcggtgt aacgtaaatc gggatctttg gttaatcgac
     1321 ctactaaagt tacattattg atcatcttta tctctccatt tcttgattca tttgttgtga
     1381 tctcttaaaa ttttgttttc gttctacttc ctcttgctcg acaattgatt gcatcagcga
     1441 atcaacagaa gttaaggtag attcgaaaga aatatctttg gcaaaatagt tttcttgctg
     1501 atatcttttc catttctccc tcaatccctc attcttcatc aattgaggca tttcctgatt
     1561 atacaaattc ttaaacactg attgattatt tctttcctta aatgttcctt gaacactctt
     1621 atacaaattt atctcatcga tacgttctag tttagggatc atatacagat catagaaatc
     1681 tttcatacgt gaattatcta ttccgaaact aaacgtggca tgtaattttt cagctaaaat
     1741 ttgttccgta ggataagctg gaatatgaat tgttttatct tcaaacatca atttatgttc
     1801 atatatcaat gctgctggaa taatagaatc acctgaagta atatctaatt taaaaggtac
     1861 acgtgcattt tcaagaaaag caataaactt tgcttgaaaa cctgggtaat aatcggcttc
     1921 cctcgtctct gttaagccct ttaattcaaa ccgaatgcca tcttttgtag gagcagctgt
     1981 taattcagaa aaaacagttt ctaatttttc ttttgttagt ttcgaatttc gataagtcgt
     2041 atcaatatca acggttgaac ggttttctaa cccgtatttt gatccgatca gaaatccccc
     2101 ttttaataca aaatcatcat gataaggcga ttcaacaata cattctaata atttttccat
     2161 catataatat ctttgaa
//
LOCUS       DACDTH010000167         2102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDTH010000167 DACDTH010000000
VERSION     DACDTH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.173"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            169..1539
                     /locus_tag="IWK54_002968"
     CDS             169..1539
                     /locus_tag="IWK54_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP9595006.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IWK54_002969"
     CDS             1560..1844
                     /locus_tag="IWK54_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595007.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          730 a          308 c          444 g          620 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 ca
//
LOCUS       DACDTH010000168         2061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDTH010000168 DACDTH010000000
VERSION     DACDTH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.174"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            676..1008
                     /locus_tag="IWK54_002970"
     CDS             676..1008
                     /locus_tag="IWK54_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9595008.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1627..1968
                     /locus_tag="IWK54_002971"
     CDS             1627..1968
                     /locus_tag="IWK54_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9595009.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          719 a          276 c          432 g          634 t
ORIGIN      
        1 tagcctttta cgtcataagt gttaaaagaa ataattaatt tcaaatatag aaaagggaac
       61 gtatgtttgc atactccttt tttttgagtt ataataatac gcgaagagag ataatttcat
      121 tatttaaaca agctaaaaat cttgatatta cttcttttca ggcaatacga atattattcg
      181 cttacatttt ttgtataaat ttaattgtct gctatgcttg tgatgaattg attttcgttc
      241 tgcacttttg gatgataaat ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga
      301 actcggaagg ctggtaacct tcgtcagcgt tcatacacgg cattaagatt tggcccttaa
      361 tgcgttaacc tttcagtgtt acggtattac ccgcaatgga tttagtataa cagatattca
      421 aaagtgatac cacttttttt ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat
      481 ctatgcctaa gcacaatgtt ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc
      541 tgaaattaga gggaacgcat gtttggatgc gttccctctt ttttgtttgt catgaacgaa
      601 aatcaattct ctatttttga aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat
      661 tgggtggttt attttatgtc atatacagga attttgagtc tcaaagatat ttgtcactac
      721 ggtaaacggt gtaccgctac agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa
      781 accgttgtgc agtgtaagaa atatataatc cagaaagaca aagtctcaga ggaaatgatt
      841 tattacattg ggaaacaaaa gcaaataatt ttaaaagatc ctatcccctt aaaagagcta
      901 tatccaacaa tcaaacatgt ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat
      961 ggagtcttgc gttgtaccgc aaaagggatg gggcgtttga tcggctgaat tgatcattct
     1021 cataacttgt attttatcgt ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga
     1081 acttagagtc actagaaaaa taaggagtag acttgatcta tcaacaattc ccttcgggga
     1141 gtgacggggt aggttttaac gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc
     1201 ttgtttggat agtggtgaaa tgaatttgtg taaataataa gcgcagagta aacaagaggg
     1261 aatttagaag ttagacaagt tagaatagcg tctagattaa tcatgagcag ctactaaggg
     1321 atctagactt tgacgcaaac tcatggaata aggagtaggt cttctcgtaa tggggaggac
     1381 gcacaagaaa ttgtgtcgtg tgctgcagta catgtagata cttgcgagga cgttgattat
     1441 tggatcaatc gagcatttac cgctgtctac tagggtttct cgtttcggac gccataaacg
     1501 agtcgaaatt ttttatgtat ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct
     1561 gaataaagtg aaaaaattga tataaaacat agagaaaatg aaatttacgt aatggaggaa
     1621 tgacaaatga aaaattatga tccgaatata cgattgggca cacatactat taaggtttct
     1681 ttccaacgtt gggattacaa aggatttgtg acgtttagaa gaggcggaaa ttgtaaagga
     1741 ttagatgttt tagcattaga cgaggatgat ttatacgatc aaacattgac tgataatcca
     1801 attggattcg gcttgctacc tgaagatgat gaaggtaatg aatggtttaa aatgacgctt
     1861 atgaatgata atggagacga attatctgtt gaagatactt ggtcatattt gagtgattat
     1921 attgtcagct ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa
     1981 taaattttac agataaaggc ttagaaggag tcacgatgat taataaaaag gtagtattac
     2041 cttaggcatt gagtaaggaa t
//
LOCUS       DACDTH010000169         2059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDTH010000169 DACDTH010000000
VERSION     DACDTH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.175"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(69..1496)
                     /locus_tag="IWK54_002972"
     CDS             complement(69..1496)
                     /locus_tag="IWK54_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP9595010.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            complement(1489..>2059)
                     /locus_tag="IWK54_002973"
     CDS             complement(1489..>2059)
                     /locus_tag="IWK54_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9595011.1"
                     /translation="FYYKHIDVYQKHDYPRINRNNTTAFKIFRAIINRCLRMIKLPPK
                     IRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNS
                     SFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKL
                     FVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          779 a          328 c          243 g          709 t
ORIGIN      
        1 aattttgata aacatttaat atattaatca aatgtaaagg agtccaagca atgaaagctg
       61 tcttcatatt aattctcctt ttttaaatac aagtaagaaa tgcttcccaa ataataccag
      121 aaaacaattg aaatgacatc gcgcttattg aaaagtacat gagcctccac catttcatct
      181 ataaaaatag atagtataaa aattaaaaca ataccaagaa acgcataatt cactttactt
      241 ctaaaaaatg ttttgagtaa actaaagata tttaacacat agaaagcata gatacatata
      301 aaagcaatta taccacttga tacaagtact tgaagaaatc cattatgcat gtttccatgt
      361 gctcttttta gttcattcac atctgtagta tcaagatcta cttttttcaa aaattttgca
      421 tcgctattcc catagaaatc ggtctctcca taaccaaata acaaagttgg tcttataact
      481 ttaagcgcag ctttccaaat cgtaaaacga ccagctgaaa tttcggagtt atcactagaa
      541 tgctgtaata ttacttcttt ttctggaagt ggagttttat ctgattcatt tgactctata
      601 acttctactt ttctatcaat cgatacagga attgttgcta aaaaatcctt agcataatta
      661 gtaccaaaag aaaaatatac caatagaact aataatatgc ttaccatcca tttgattttt
      721 acaggtagta cttctctaat gctctttcta aattttgaat tggcaaacaa caaaagaaat
      781 aatactaaaa aagaacaaat tactacctgg gcacctctag acgaagaaag tgtaatataa
      841 aatatttgta cgataaaatt catcatataa aaaacacgaa tcgtttttgt tatttttgaa
      901 attaatacac taattaaact taaaataata gaagtatatc caaacatagc tccaacattt
      961 ggacttgtat agacaccaaa taaccgatgt tcaataaaac cttgtcttgc aagtaatcct
     1021 ggcgttcctg ttgggacatg ataagaaatc aaaaaaataa attgatagat agatattagc
     1081 gcagctaaaa aaattatgat aatgaaaaca ttattaaaat acaccatttt tttctttttt
     1141 tcctcttcac tttgtgaaaa gtctgcagga aaaattacta tcaaagtaat cagcaaatat
     1201 ccaaaattat aaatagatgg aattaaaaaa ttttctctgt taatgattat agttaagata
     1261 aaactaatca ttgctccaat cagtagccaa gaaaaacgca ttttaaaaaa atttttcttt
     1321 ataaaaacct catacaaaac tataattcca ccccaagcaa aagctatttt catcgggata
     1381 ttagttacat taaaagtact aggcataaaa taaagcagac aaatgattaa atttattact
     1441 cgaaacgcat ctcgacttaa taaactcttc tctaaaacag gtactattct attcatctat
     1501 atttccaact ttcaaaattt ctttatcaat tctatttaat agtttatcag agtaatccga
     1561 tttaactttt cttacaaata atttttcact ttcttttatt tgatctatat ccctttcatc
     1621 aaaccatttg ttcatggaag gatcaataat atgaatattt gctaatttcg aagtttgtaa
     1681 agtagtttct tcaatatttt tcaattcaag tggtaaatct ggtcctccaa taacattttt
     1741 atttttgaac gaggaattaa aaaaaagtgt atgaaaaaat ttttcatctg gggcaaacat
     1801 acctatttta aaattcaggt agtcttgttg attttgttca tacatttgta ttaattctgt
     1861 taggcattct ttcgacaaag cccaccattg agaaccatga tacaaatcaa atttatggtg
     1921 tctaattttt gggggaagtt taatcatcct cagacaacga ttaattatag ctctaaaaat
     1981 tttaaatgca gttgtgttat tcctattaat tctaggataa tcatgttttt gatatacatc
     2041 tatatgttta taataaaat
//
LOCUS       DACDTH010000170         1980 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDTH010000170 DACDTH010000000
VERSION     DACDTH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.177"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(53..478)
                     /locus_tag="IWK54_002974"
                     /pseudo
     CDS             complement(53..478)
                     /locus_tag="IWK54_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
     gene            complement(587..1618)
                     /locus_tag="IWK54_002975"
     CDS             complement(587..1618)
                     /locus_tag="IWK54_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9595012.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          609 a          333 c          341 g          697 t
ORIGIN      
        1 caacatgttt agacattttc ctctaggaaa aaattaacta gcaccacggg ttaggaatct
       61 gttttgattt tgttccattt gaaatttctc tcactcgctt ttttgtctca tcatcaaata
      121 aaatgaaagg gtcttctaaa cgatcgataa atacttccat tgttgttgtt ggtagatgat
      181 aagaagtcat gacaggcact gcaccaaggt aagacacaga aacagctaga aggtatgtgt
      241 caaaagcgga actcttataa ataataactt tgtctccgga tttcactcca agatgagcca
      301 attgataagc ccgtttcaac acactttcgc agcatttttt gtaagttgtt tcaagtccta
      361 attctgggaa agctgaaagt ggctcatcaa agataatcgg ggtctcggaa aagttagctg
      421 cagcttcttg aaaattcgta aataagttca aaggggaata cgttatggtc tccatcattc
      481 acactccatt atcttttgtt aagtaatata caaattcaag ataatcgtaa cataatcctt
      541 tatataataa catagtttat tgtgaaaaaa agatcataca tttgttttat atggaagaaa
      601 cccgaacgaa tgccgctaat tcgtgataag aaaaatcaac catcttttcc cagatatctt
      661 gaaaagaatg ggatgagagg tatcgattac cacataaata atttaccgcg tcagcatcat
      721 ctaaatatag ttctgcataa taacgatatg tttttctgca tagttctggc gatacgtttt
      781 tatatactgt aattttgctc aaaatttggc gaatagtccg aggagaaatt tgctgatcat
      841 gtgaggaacg aaaaacaaaa gaattttcgt ccgtattttg tgctgttaac ttaaaatagt
      901 tctccagaaa tggcatcgct tcttttggta ttggtacttt attcagtctg ttttcttttc
      961 tattcagatt aagaatctgc ttggtcaaag atacatcttt acgtttaagt tctactactt
     1021 catggatctt taatccgcaa tacagaagaa tggcagtaat tgcaaggttc ctcggtctat
     1081 tcttttcttc aaagctagct tctctgcttg tttgaaagcc ttcaatcggg ataaacaaat
     1141 gctcaaaatc tttgaaaagt tctgcatcga tatatcgcgg agctgctttg gttgtttccg
     1201 gtaaacttgt acccaaaaga gaaatctctt cttcaatcag tcctttttga tacaaaaatc
     1261 gtaaaaaatg atttaatgaa tattgcttcc ttgaaatccc gtaatagcta ttttcaaaca
     1321 attgctccgt ttcttggttc gctgtagttt ggaacgaacg agtatgtcga tagattttag
     1381 ctaagaaatt ctgataatct tgtttttcta aatggataat cagctgatca tccacttctt
     1441 cagaaaatac ttttctcaaa taatcagtaa acagatgaaa atcttttgcg tattcatagc
     1501 gactgcctaa tgaaagggaa gaacgatttt cttctaaaaa ctgttgaaag acggaaggga
     1561 gcaagtgtgt acgttgcttt aacttttctt gcagttgttt tctagttttg ctgtccatta
     1621 caaattccct ccaatagtgt tcattttcta tattttaccg catttcatca aatgaagata
     1681 gacagtttgt cttgatctta ggaagcaaat ttttaactaa gtactagcta aaagatatga
     1741 agtaagaaga tacattttta gttattttat tagtgcagtc attcttttga tcttttaatt
     1801 gatttatcct ctttttagct atgtggttac ttcatgagca atgaaatgga tttggttcgt
     1861 tctaatggca ggaggctctt tcttttattt ccgaaaaatt agaaaacatt ttttcagaat
     1921 cgattggctc tccatttatt caataatggt ttgtctaacg atattgttgg gataattctt
//
LOCUS       DACDTH010000171         1972 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDTH010000171 DACDTH010000000
VERSION     DACDTH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.178"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..110
                     /locus_tag="IWK54_002976"
     CDS             <1..110
                     /locus_tag="IWK54_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9595013.1"
                     /translation="YEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            111..863
                     /locus_tag="IWK54_002977"
     CDS             111..863
                     /locus_tag="IWK54_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9595014.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            868..1815
                     /locus_tag="IWK54_002978"
     CDS             868..1815
                     /locus_tag="IWK54_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9595015.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          690 a          264 c          398 g          620 t
ORIGIN      
        1 tgtatgagaa ggagttgaaa tatttctttg aaaacgtagt aacaggcaaa gaaaactgga
       61 atgatttata tcatgcaaac gaagtattga aaatagctga ggagaaataa atggaaattt
      121 tatttactat ttgtggtaga gctgggtcta aggggtttaa aaataaaaat ttgaaatatt
      181 tggcaggaaa gcccctagtc catttcgctt tagcaatgat agatatctat caacaaaaac
      241 atcctgaaca taattgcacg acttgtttga atacggacag taatgagtta gtagaattga
      301 cgactaagtt aaatccgtct gtgaaaattg tagaacgtaa acctgaatta gctacagata
      361 ctgtagggaa aattgaagtg atacgtgatt cattcttaca actagaaaga gaagataata
      421 tatttgattt aattatagat ttagatttga cttcaccttt acgtgaagtt tctaatcttg
      481 aagaattaat agatgaacat ctaaaaaatt tagataaaga cgtctacttt tcagttgttt
      541 ctgcaagacg taatccctat tttaatatgg taaaaagaat tgaaaatgaa gttactatag
      601 ttaataaatc aagatacaca tctaggcaac agacacctga agtttttgaa atgaatgcct
      661 caatgtattc ttttaaacct gaatttctaa aaaaaagtaa ttatattttt gatgggcaat
      721 gtgggataat aaaaatgaga gattatctaa ttttagatat tgatagtgaa gaagatttta
      781 aatggttaga atttttgttt cctaaatttt tagaagattc ttcggagctt caagatatct
      841 ataattattt atatcggagg taaaaggatg agctgttatc ttatcaccgg tggggctggc
      901 ttcattggtt ctaccttagc caatcattta ggagaagaac atgtggtaat cgtcgtcgat
      961 gacttatcaa tgggaaaagt agaaaatctt gatatgacga aaaatatcac ttttattgag
     1021 ggagatgtcg ctgagaaatc cttgatggaa gagatcatga aagctaataa atttgattat
     1081 atctttcatt tagcagcagt tgctagtgtg gcagattcag ttgcccgacc agtagaaaca
     1141 catcgtgtga attttgaaag tgtattaatg attttagaat tgatccgcaa gtatcaacct
     1201 gatttaaaac ggatcgtgtt tagttcttca gcggctgttt atggggatga accgacttta
     1261 ccaaaaaaag aagagtccgt gattcgccca ttgacgcctt atgcggttga taaattcgct
     1321 gcagaacaat atgtgctaga ttattgtcat ttatatgatg tacccggtag cgcagttcgt
     1381 tttttcaatg tctatggtcc aaatcaaaat ccgaattctc catacagtgg tgtgatttct
     1441 attctcgtag atcgctataa gaaacaaatc gtaggagaag caacttcatt tacattattt
     1501 ggtgatggaa gccagtctag agactttgtt tatatagatg atgtaatcca agcattactt
     1561 ttagttgcga atgaggaagc tgcactgggg aaacaattca atgttggaac gggtaaagca
     1621 accacacttt tggaattgat tcatgcaatc aatgaaattc tcggtgtaga attgacactt
     1681 gaatacaaag aagagcgttc gggtgatatc catgactctc tagcagatat tactaagatc
     1741 cagtcaattg gctatcaacc gaaatatggt attttaagtg gaatgactaa ttatttgaaa
     1801 actgaaatca attaaaacat actaagataa gattagaggg taaattcagg ctctttgtca
     1861 ataaggactg atgagttttg taaaatcaaa tctgggatag aagggctctt tgtcaaatga
     1921 tgttggtaaa gagaaatctt agaataattt ggacagaaca gaaagaagaa aa
//
LOCUS       DACDTH010000172         1948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDTH010000172 DACDTH010000000
VERSION     DACDTH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.179"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            138..1751
                     /locus_tag="IWK54_002979"
     CDS             138..1751
                     /locus_tag="IWK54_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9595016.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          621 a          340 c          400 g          587 t
ORIGIN      
        1 aaaattttta ggttttatcc acaaattatt tctctatatt ttctgtctat tcaaaataaa
       61 caataatttt ccggcacaat tatttgctgt tttttaccat aacatagtac gatcaacgta
      121 gaaaaggaga tgtttttatg aaagttgttg tcattggcgg tgtagctggc ggtccttcat
      181 ttgccactcg tttccgtcga ttgaatgaag cgcacgaaat cattatctat gaacgcggag
      241 aaaatatttc ttatgcaagt tgtgctttgc cttattattt aggaggtgtg atcactgacc
      301 gtgactcgct gatcgaacgt acaccagaaa tattgaaaac aaaaaacaac atcgatgtct
      361 ttactaaaca cgaagtaaca gcaatcgatc cttctactaa gcgattaaca gttaaagacc
      421 tatccacaaa tgaagaaata aaaacagatt acgataagtt gatcatctct tctggtgcta
      481 gaccagatta tccggatatt cccggagttt ttgaagcaga aaacggcttt gtcctccgta
      541 gtgtgacgga tgcggatcga atcaaatcgt tccttgaaga aaaaaatcca caacatgtcg
      601 tcattcttgg tgcaggtgtt atgggtctgg aattagctga gaatttcaag catcgcggct
      661 tagacgtgac tttaatcgat caattgccac aagtcgcttt cccttatgat ccagaaattg
      721 ctaatttagt ttatgacaaa ttgctgaaag aaggattagc cgttcattta gaaacaagag
      781 ttactgagat ccgtgataaa ggtcgagaaa tagtattatc agatggctcc gtcctttctg
      841 ctgatatgct aatttttgct gttggtgttt ctccgaagaa tgaagtggtg aaagcaaccg
      901 gcatacaatt atcagataca ggtcagatca ttgttgatga ccagttacaa accaatctgc
      961 cggacatcta tgcgattggc gatattatcg aaacgactag tgtagtgact ggtcagccga
     1021 tccaaagtat gctttccagt gcggccaatc gtcaaggaca tatgctggca gatattttaa
     1081 atggtacgcc tatgcgctat cgcggatata ttggtgcagg tgtcgcaaaa atctttgatc
     1141 atacagcaag ttatgcggga atgacagaac atgaattaaa agcatcaggc ataacaaatt
     1201 ataaaactgt ttttatcact ccctttgacc atgcctattt ctatccagaa gctacaagat
     1261 taaatctaaa gctgattttt gatgcagata gcggtcgtat tttaggtgga caagcatttg
     1321 gagaaaaagg tgtcgataaa cggatgggag aactttctgt agcaatcacc ggaaacttga
     1381 cagtctttga tttgcctgat ttggagttgc cttactctcc accgtattct actacccgtg
     1441 acccgttgaa tatagctggt tatgtcgcaa tcaatcaaat gacgaatatc gtagaaacga
     1501 tcaaagcaag tgatataccc gaaaacgatt tgaaagaagc gttcttttta gatatacgtg
     1561 aacctaataa agcaccttct ggcagtatct cggcaacaaa aaatattcca atgaatgagt
     1621 tacgagatcg aatcaatgaa atcccaaaag ataaaaaagt ttacattact ttcagaagag
     1681 gattgaatac ttatacttct gcccgaatct tggcaggttt gggtatcaaa gcgattttga
     1741 ttgaagaata aaaaaacgac cgcggtgggt agccgcggtc taggagttat ttttacttga
     1801 ggagtaaaaa tgaaaaagta tatagggttg ttggttggta tgtatataga atagaagata
     1861 aatgtgaaga aattgtgcat tatttcttca catttattag gttatagttt aagaaagttt
     1921 aaacattgcg caatttaatt attttagc
//
LOCUS       DACDTH010000173         1845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDTH010000173 DACDTH010000000
VERSION     DACDTH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.180"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..565
                     /locus_tag="IWK54_002980"
     CDS             <1..565
                     /locus_tag="IWK54_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595017.1"
                     /translation="DVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLL
                     SDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLEND
                     FSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEH
                     YNRLSEEKILLPENWAELRKLVEYGII"
     gene            552..>1845
                     /locus_tag="IWK54_002981"
     CDS             552..>1845
                     /locus_tag="IWK54_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9595018.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIF"
BASE COUNT          620 a          235 c          284 g          706 t
ORIGIN      
        1 tgatgtaaaa aatataaaaa aaattcccaa aatagatgaa aacagtaaat tgatgaaaaa
       61 catacaatca atctttggga tagaagaagt ttttgtcgga gataaatgta tcttttttga
      121 tcaaccttta ttatctgatg gttatggcat agatgaaaca gatattttcg ctaaagtaaa
      181 tagtatattt tctggaaaaa aagaaacttt aattaagctt catcctagag caaggacaaa
      241 tcgttatggt gaaaaaaaca tattgaattc cagtttaccg tttgaagtct tgctacttga
      301 aaatgacttc tcttcaacac ttttgattag tcctatatct acaatttctt tttcaccatc
      361 tatgatgttt gaaaagacga tcaactctat tatgttggct aaattaattt taaatgaata
      421 tacagcatta tctgaagata aaaaagaaac attgacaaat attgttcttt tctgtgaaca
      481 ttataaccgt ctatcagaag aaaaaatttt actcccagaa aactgggcag aattaagaaa
      541 gttggtagag tatgggatta tttaacacac tttccaagtc aaaattagtt aagtccagtt
      601 tctggtatac tatagggaat ttttttatta agggaatttc attcataact attcctattt
      661 ttgtaaatat tactactcaa gcagaatatg gtttgattaa taatttcact tcatttgtat
      721 caattttttc gattcttgtt gggctaagcc taaattcggc aattaataat gcaagttttg
      781 actataaaga tgacataaat gaatttatgt catcggtact ttctctagct acactttcgt
      841 taattgtgtt cttattaata gggaacatat attttttgtt ttcaaatgct ttttttgata
      901 tgaaccaact tgtatttaat ttgatgttat tacaaagttt tgctagtttt gtaattagtt
      961 atatccagtc ttattttacg attaacttta accattttaa atatttgatt ttatctttta
     1021 tatctacttt tttgaatatt gcaatctctg tagctttgat gcagacatta tattttgatg
     1081 ataaatatgt tggcagagta gtcggcggaa cactgtcatt tgtgttgata ggtttagtaa
     1141 tttatttaag aataatgata aagggtgctc atttttttga taaaaaacag tggagatacg
     1201 ctttaaaaat ctctttgccg ctaatcccac attccttagc aaatatagtt ttagcacaat
     1261 tcgatagaat tatgattaat tcttatttag gtcctgctgc ggggggaata tattcctaca
     1321 ttagtaacat aggtattatt ctaagcgtaa tatggatgtc tactaataac gcctgggttc
     1381 catggttcta tggtgagatg agtaaaaata atgaaaaaaa agtgaagaaa gttagtaact
     1441 attatttagt agtctttact ttgattacga tgattgtaat ggtagtttcg attgatttag
     1501 caaaatttat ggctccgaaa gactattatt ctgggttatc tttagtggtt ccgataacct
     1561 taggctattt ctttcagttt ttatatagtt taccagttaa tgctgaattt tatgagaaaa
     1621 aaacatcgtt tattgcgatt ggtacagtag caagtgctat agttaatata ctattaaaca
     1681 tgattttcat acctaggtta ggaataattg ctgctggata cacaaccgta tttacgtatt
     1741 ttttactttt tatttttcat tataatatcg ctaaaaaaat tacgaataaa caattatttg
     1801 acacaaaaaa aatatgtatc ataataattt ttgtatcaat ttttt
//
LOCUS       DACDTH010000174         1796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDTH010000174 DACDTH010000000
VERSION     DACDTH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.181"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="IWK54_002982"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="IWK54_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9595019.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DACDTH010000175         1660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDTH010000175 DACDTH010000000
VERSION     DACDTH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.182"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            138..752
                     /locus_tag="IWK54_002983"
     CDS             138..752
                     /locus_tag="IWK54_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9595020.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(776..1126)
                     /locus_tag="IWK54_002984"
     CDS             complement(776..1126)
                     /locus_tag="IWK54_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9595021.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1120..1383)
                     /locus_tag="IWK54_002985"
     CDS             complement(1120..1383)
                     /locus_tag="IWK54_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP9595022.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          558 a          294 c          256 g          552 t
ORIGIN      
        1 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
       61 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
      121 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      181 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
      241 aatctggcgc aacgattact catcgacctg tttttcaaga agcactggat ttcgtccgag
      301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      361 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
      421 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      481 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      541 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      601 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      661 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
      721 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
      781 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
      841 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
      901 ataatacttt tcctgttgtc ttcatttcat attctgagtt tatatttaca tatagcggat
      961 agtttctagt tgtactagaa attggagcaa ctgtaacaac gttactataa tccgcaataa
     1021 tatcatgact taaaacaata taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg
     1081 ctgtattcaa taatagaata tcgccttgtc taggttttct caccattttt cattacctac
     1141 tgcttcacct aaattttgaa tcgtcacatg atttctttca tcagtgtaat tttcaaataa
     1201 ctgttcaatt gttaattcat tttctatatt agccgcttcg ataattagcc gcttctgacc
     1261 gtttacttct tcttgtatta tcttcacttt gtcttctgta tgaattccaa gatattccat
     1321 aatatacttt ggcaatctaa ggccattact atttccccat ttttttatag gtaacatttc
     1381 catagtagac acctctttct gtatatacat agtataacat acataagaaa aaacgtatat
     1441 acttaattat ccttttctat taaattagat taacattcca tgaatccata aactatgtta
     1501 acatttatat gaaaatagtc gaaaggaaat cataatatgt ttaagaaaac aaaatcttta
     1561 gaatcaggta agaaaaaaat tgatctaaaa ataatctctt ctggatactt taaagatttc
     1621 atagttattg atacaaaatg gaataaaaag gacatagaaa
//
LOCUS       DACDTH010000176         1596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDTH010000176 DACDTH010000000
VERSION     DACDTH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.183"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..626)
                     /locus_tag="IWK54_002986"
     CDS             complement(<1..626)
                     /locus_tag="IWK54_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9595023.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
     gene            914..1567
                     /locus_tag="IWK54_002987"
     CDS             914..1567
                     /locus_tag="IWK54_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9595024.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          572 a          224 c          299 g          501 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga taccttttat
     1081 aaatggttta gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt
     1141 gactgcattt agtgatgaag aatataaaat tgatgcattt actaatttag tagacggata
     1201 tgtagaaaag ccattttcat tgccggtctt aaaagctaga atagattctt taattaagaa
     1261 gaattatgga catttagaga agtttgaata taaggatcta tcggttaatt ttaatagcta
     1321 tacagctaaa ataaatggtg aagaaataga tgtaaatgca aaagaactag aagtattaaa
     1381 atgtttattg gataataatg gacaagtgtt aacaagaatg caaattattg attatgtatg
     1441 gaaagatagc gaagaaactc cttatgatcg agtaatagat gtatatataa aagaacttag
     1501 aaaaaaatta caattagact gtataactac tataagaaat gtaggatata aattggagag
     1561 aaaatgaaaa aattaaagat atttccaaaa atgttc
//
LOCUS       DACDTH010000177         1586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDTH010000177 DACDTH010000000
VERSION     DACDTH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.184"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            76..1542
                     /locus_tag="IWK54_002988"
     CDS             76..1542
                     /locus_tag="IWK54_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9595025.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          441 a          291 c          332 g          522 t
ORIGIN      
        1 ataagttttt tagttaaatt tttcggtaaa gatgtataat attttctgaa aatttaaaga
       61 attggggtat agattatgga aaaacagcaa gacaagggct tcttcgggca gccgaaggga
      121 ttgtcaactt tgttcttcac tgagatgtgg gaacgattta gttattacgg tatgcgtgca
      181 ttacttatct attatatgta cgatacagtt gccaacgggg gacttggatt accacgagca
      241 acagcattag ctatcatgtc gatctacgga tctttgatct acatgtctag tatcgtcggc
      301 ggatgggtat ctgaccgagt gttaggagca aaacgcactg tattcttcgg tggagttttc
      361 attatgcttg gccacatcgt cttggcttta ccatttggtg taagtacatt gtttgtttcg
      421 atggcattga ttattatcgg aaccgccttt ttgaaaccaa atgtttctgg catggtcgga
      481 catctttatt caaagactga cttgcgaaga gatgcaggat tttcgatttt ttacatgggt
      541 gtcaacttag gggcattatt agcaccacta atcgttggta caatcggtca agaatataac
      601 tatcatttag ggttctcttt agctgctatt gggatgttct ttggattatt acaatacatc
      661 atccaaggac gcaagacatt agacggagtg ggtacaacac cgccgaatcc tttgacaact
      721 gaagaacgta aaggattcat ccgtaacttg atcatcgcac ttgttgttat cgctgcagta
      781 ttcggcgggg cacaactaac tggtcatctg acaattgact tttttgtcaa tacaatcagt
      841 gtattgggga ttatcttacc gttgtattac tttatcaaaa tgctgacttc aaaagatgta
      901 actgctgaag aaaaaccaag agtatgggct tatatcccat tattccttgc agcaatcatt
      961 ttctggtcat tacaagaaca aggttcatct atcttagctt tatttgctag tgaacgtaca
     1021 caatctacgc tatttggtgt aacgatccaa gcaagctggt atcaatcatt gaatccaatt
     1081 ttcatcttta ttctgacacc agtattcgta acgatgtgga cgaaacttgg tgaacgtcaa
     1141 ccatctacag tagtgaaatt ctctcttgga cttttatttg caggattatc gtacttgcta
     1201 ttgatgttgc cgggtatgct ttatggtaca agtggaaaag taagtccatt gtggttgttg
     1261 ggaagtttct tcatcgtggt tattgctgaa ttgtgtcttt caccagttgg tttgtctgtg
     1321 acaacaaaat tagcaccacg agcatttgaa tcacaaacaa tcgctatttg gttcttagct
     1381 gatgcttctt cacaagcaat caatgcacaa attgcacgct tctacacacc gggaacagaa
     1441 tctgcttact tcggtattat cggtgcagta gcaatcgtcg gtggtatctt gctatttact
     1501 gtgaaagaac caatcaaaaa actaatgggc tctattcatt aatcaatgaa cataaaaaca
     1561 ggaattgctt atttgcagca gttcct
//
LOCUS       DACDTH010000178         1573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDTH010000178 DACDTH010000000
VERSION     DACDTH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.185"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DACDTH010000179         1572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDTH010000179 DACDTH010000000
VERSION     DACDTH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.186"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..861
                     /locus_tag="IWK54_002989"
     CDS             <1..861
                     /locus_tag="IWK54_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9595026.1"
                     /translation="EGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKI
                     KSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYS
                     GSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGM
                     VVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNVLL
                     KVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFASSLMLIK
                     KPKEILSKIS"
     gene            871..1527
                     /locus_tag="IWK54_002990"
     CDS             871..1527
                     /locus_tag="IWK54_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9595027.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
BASE COUNT          651 a          144 c          266 g          511 t
ORIGIN      
        1 gaaggtaaaa atatagaaga aaatgataag aattcaatta ttgttcatga agaatttgct
       61 aaacaaaaca atctaaaatt aggtgatgaa gttgatcttg aattactaga tattgaaaaa
      121 agtggaaaaa taaaaagtca taaatttaaa attataggga tcttttctgg taaaaaacag
      181 gaaacatata caggattatc ttctgatttt agtgaaaata tggtgtttgt agattattca
      241 acaagccaag agatattaaa taattcagaa aataatgaaa ttgcaaataa aattttaatg
      301 tattctggta gtgcagaatc tacagactta gccttaaaca aattgaaaga gcttaaaatt
      361 gatgagtcaa agtattttgt tgaaaaagat tctaatgcgt ttgaagagtc tttagagtca
      421 gtgagtggaa taaaatatat aataaaagta atgacttatt ctattatgtt aggtgggatg
      481 gttgttcttt cattaatctt gattctatgg ttaagggaaa gaatttatga aataggtata
      541 ttcttatcta ttggaacatc taagatacaa attataatgc aatttatatt cgagttaata
      601 ttcatatcga taccaagtat aatatcttcc ttatttttag gaaatgtatt actaaaagta
      661 attgtagatg gattcattaa ctcagaggat tcaatgattt ccggtggaag tttaataaat
      721 aatagtagtt ttatgtcaaa tataacaaca cttggacaaa gttatttaat attaataagt
      781 attattgttt tatcagttgt atttgcttct tcattaatgt taatcaagaa gcctaaagaa
      841 atcttatcaa aaataagtta ggagaaaata atggatatat tagaaataaa gaatgtagca
      901 tatagttatg caaattctaa agaaaaagtt ttgtcaggag taaatcaaaa atttgaactt
      961 gggaagtttt atgcgatagt aggaaagtca ggaacaggaa aatctacact tctttcctta
     1021 cttgctggac ttgataaacc tcaaacagga aaaatattgt ttaagaatga agatatacaa
     1081 aataaaggat atagtaatca tagaaaaaat aatatatctt tagtatttca aaactataat
     1141 ttaatagatt atttatcacc aattgaaaat attagattag taaataaatc agcagatgaa
     1201 agtatcttgt ttgaactagg tttagataaa aaacaaataa aaagaaatgt tatgaaatta
     1261 tctggtggac agcaacaaag agtagctatt gctagagcat tggtatcaga tgctccaata
     1321 atactagctg atgagcctac tggtaatcta gacagtgtta ctgctggaga aataattaat
     1381 atattaaaga cattagctaa agatagaaat aaatgtgtaa tagttgtaac acatagtaag
     1441 gaagtagcag attctgcgga tatcatttta gaactaagtg gtaaaaagtt gaaaaaagta
     1501 aataaaatga atttggaggt tgaataatga taaaaaatgc atttgcttat gtaactagaa
     1561 aaagtttaaa at
//
LOCUS       DACDTH010000180         1525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDTH010000180 DACDTH010000000
VERSION     DACDTH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.187"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            161..1093
                     /locus_tag="IWK54_002991"
     CDS             161..1093
                     /locus_tag="IWK54_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP9595028.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1165..>1525
                     /locus_tag="IWK54_002992"
     CDS             1165..>1525
                     /locus_tag="IWK54_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9595029.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYY"
BASE COUNT          673 a          172 c          236 g          444 t
ORIGIN      
        1 tatgttaaaa aatgatatta ttttattaaa aaaagagaaa gaagatttaa atgatgaaat
       61 aaatcaaaag tatgatgaaa cattaaaaaa tgtctattct tcacttatcg tttcaaatat
      121 agaagaaact tcatcttctg aaataaaaaa taagctttct ttgttaaaat taaaagaaaa
      181 ggaatatatt attgacgatg ctgtttctgt ccctcttatt ttaaataaac gagagaaaaa
      241 taatttaaag aaaaaaatgc tcattccatt tgattcagaa gtgaaaggat tactcaataa
      301 actaactatt tctaatatag actcgactag ggaaaaaatc ataaaaatat ttgataaaat
      361 taataattta ttcaagaatg acaatgctga attaaggaag ggtcttttag agtttaaact
      421 tcaagaacta gaacttcatt actcttattt agtgaaaatt accgaagaaa aagaacaaca
      481 aaaagcaata aaagaacaga tgatagaaga ggaaaaagtt cgtagagaaa ttgatcgtga
      541 aaagaaacgt attgataaag aacaacgtca attcaattct gaaatttcca aattaatcgc
      601 atatatgcaa aaagctaatg cagatgtgga aaaagaatta tatgcaaata aaattcaaga
      661 actggaagaa aaattaaaag aattagaagt tatacgtgaa aatgtattac aaagagaatt
      721 aaatactaga gccggttatg tgtatgtaat tagtaatatt gggtcttttg gtgaagacat
      781 atataaaatt ggtatgacta gaagattaga acctatggat agaataaaag aattgagtag
      841 tgcatcggtt ccatttgaat ttgacgttca tgcgatgatt ttttcagagg acgctccgtc
      901 tttggaaact aaattgcata atcattttag aaaacaagaa gttaataaaa ttaatcaaag
      961 aaaagaattt ttcaaagttt cactagacga aatagagaaa gttgttttag aaaattataa
     1021 tggaacagta acatttacaa aattagctaa agctgaacaa tatagacgat ctttagaatt
     1081 gtcaaaagat taaatatata aaaagccttc gggctttttc ttttaacaat taataggaac
     1141 gtatattcga aaggaataga aataatgtgg ttagaaaaaa taaatgacaa aaaatttaag
     1201 tacaccgaac gttatacgga tcccttaact gaaaaaaaga gaaaagtgtc cgttacttta
     1261 tctagcaatt cacgccaagc ttggaatcaa gctaacttac ttttgaatga aaaaatcgca
     1321 gaaaaaataa aacgaaatga agaattacca ctaacttttg gagaattaaa aactaaatgg
     1381 gatgaaaaat acaagccaac tgtaaaagaa agttcctatc gaacaactca ggtttatcta
     1441 tcattaattt caaaatatat aaaagatgat gtacttgtaa aaaacgttaa tagtaatctt
     1501 atacaagata tgctagatta ctact
//
LOCUS       DACDTH010000181         1509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDTH010000181 DACDTH010000000
VERSION     DACDTH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.188"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            101..868
                     /locus_tag="IWK54_002993"
     CDS             101..868
                     /locus_tag="IWK54_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9595030.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="IWK54_002994"
     CDS             1060..1458
                     /locus_tag="IWK54_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9595031.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DACDTH010000182         1505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDTH010000182 DACDTH010000000
VERSION     DACDTH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.189"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..493)
                     /locus_tag="IWK54_002995"
     CDS             complement(<1..493)
                     /locus_tag="IWK54_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9595032.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLE"
     gene            637..1413
                     /locus_tag="IWK54_002996"
     CDS             637..1413
                     /locus_tag="IWK54_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9595033.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT          507 a          225 c          270 g          503 t
ORIGIN      
        1 cttcgagatg accaaattct ttagtcaggt gtggatagag actgttccgt tcatgaatcg
       61 ttctgcgaaa ggcctgtttt ccacgctttt cttgatgatt attcggttta cgtttcccct
      121 tcataggtaa cgactgtgtg tcaaaaaggc cttttttaaa catccgatag agtgtacgaa
      181 cagaacaatc aattggaaac tcgccacggc cgataatcac atcgggtgac catccttgag
      241 ctaccttttc ttgaacatat gttgattgtt catcagataa aacggttgaa cgtcgtccac
      301 aacgagattt attttgtttg tattgacggt aataatctag cacttttttt ccttgtttga
      361 gaaacgaata aacattataa atggtttgtc tagagcgatt taaactttgt gcgacaagag
      421 cgacaggttt ctcgatattg aaataagact ctatgattac tagttcgtct gttgtaagat
      481 gggtgtaagt catttgtact cactccttat taattctttg gtcggaacta ttttgagtgt
      541 agcacaaatg atctttttag ttgtctagct taattttaca atcgacgttt taataaattg
      601 tgatataatg aagtataact ttttagggag tgtactatga attttcaaga taatagacca
      661 ttatatttac aagtagaagc ttctatacgt aacgatattc gtgaaaaaaa atatcttcca
      721 ggagagcaat tacctacaga agaagaatta tgtaaaattt atggagtaag taagattacc
      781 attcgaaaag cttttaaact tttagcagaa agtggaacag ttgaacgatt aagggggaaa
      841 gggacctttg taactcaaaa aaaagaaagt ttaaaattag ctggagttca tggatttagc
      901 gattctcttt ctacccgagg acataaaatt agatatactg ttttacattc taaaattata
      961 aaggctgatg agtttttagc taataaatta caaatcaatg taactgatag agtttacaat
     1021 attaaacgtt taatgtggga agacggtgcc ccgatgggaa ttgataattt tttcgcatca
     1081 gaagaaaaat ttccaacttt atttgaaaaa ggtagccaat atacttcatt atataaactt
     1141 ctagaagatg actatgatgt caaagctacg agctctatta tggaaataaa cggtttatct
     1201 gcaacacctg agctttccga cttattgcaa tgtatagtag gagatccgct atttatttta
     1261 gaaaaggttg gcaaagataa aaataatcaa ccatttcatt attctcaatc cacagttcgt
     1321 tgtgatagaa ttagttatgt tattgaagtc acagaaaata tcactttaga tacggtgcat
     1381 aaaaatgatt cagatcctaa agtaatagat tgatattaaa agaaatcata cccaaagcaa
     1441 tcaattttga ttagatttgg gcatgatttt tatttttact aatattctct aaatacatca
     1501 aaaaa
//
LOCUS       DACDTH010000183         1489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDTH010000183 DACDTH010000000
VERSION     DACDTH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.191"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(42..1181)
                     /locus_tag="IWK54_002997"
     CDS             complement(42..1181)
                     /locus_tag="IWK54_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9595034.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          390 a          320 c          266 g          513 t
ORIGIN      
        1 aatgctaatc tggctctgtt ttttcattta ttaaaaaaat cttacgctaa gtaggataaa
       61 ataattcctt gatatttttc aatcatatcc aaataattat cgatttcaac atactcatct
      121 acttgatgag gcaaagtaac gacaccaggc ccaaagacga caaagtcgaa ggaatggtta
      181 gctttcgtaa attcggctgc atccgttgtt gctgccgctc caactaaagg taatggctgg
      241 ctgaattgtt gctgaatgca atgaatcaaa ggagaatctg gatcagcttt gacaggtatt
      301 ttgttgtaat cgatcatcaa ttccaaatga tagtctgtct cttgattcaa ttcattgacg
      361 attgattgca gcaatgcaat gattttatca tttgggtatt ctggaatcga tcgaatgttc
      421 ccttggagct gcgcatggct tgggatgctg ttgacttgat tcccgccact gatcaatgta
      481 acattatgga ttgtttttcc taggacagga ttctcgattg tttcagcgag gtgattcatt
      541 tcagcatttg ctttcgtaat gaattcattt aggtgattga ttgcattata accttggtct
      601 ggcatagagc tatgcgcttc ttttccgtgc gaggtgactg tatagttgat tgatcccatg
      661 tgcgtataca tcaaactgta attagttggc tctccgatga tcaaggcatc taagtcatct
      721 acatagcctg ctttcgtgag ctgttcccca cctagttcac ccacttcttc tccgacagtg
      781 gctaataatt tgactgttcc gttaaatggt tttcctgact ctttcagttc gatcatggca
      841 atcaccattg ctgccaagcc actcttcata tctgtagcac cacgtccgta caaacggttc
      901 ccatggattt ctgctgcaaa gggtgcatag gtccaagacg attcatcacc agccgccacg
      961 acatccatat gcccagatag gcctaacact tttcctgatt ggcccttttg gtaacgagca
     1021 atcaaattat ctctaccatc tctgtaagaa acaatctctc ctgttatgtc atgctttgcc
     1081 aaaagcttat tcaaataggc tgctacttca ccttcgtttc cattcactga tttaattcgg
     1141 atgatctctt gtaaaatcgc gattttttct tcttttttca tctaatcctc tcccttgcca
     1201 tttcttttta ttgtatttga gtgtgctcga attttcaaat aggagcaacc aaagtaaaaa
     1261 tgaagaaaga gagccacttt atgaaaaata tgctgctact tccctcctat tttatttata
     1321 cgagaggact tgctcataga ttcctattct actgttctat ccttaaaagc tcttatatga
     1381 tgctagttat tgttagcttt cctttttcaa ggatgatctt ttcttgtacc attctttgta
     1441 aacatcatat acagaagcta gttgcaaaaa aaactctcta cctgctgat
//
LOCUS       DACDTH010000184         1451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDTH010000184 DACDTH010000000
VERSION     DACDTH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.192"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..625)
                     /locus_tag="IWK54_002998"
     CDS             complement(<1..625)
                     /locus_tag="IWK54_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP9595035.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="IWK54_002999"
     CDS             complement(784..1050)
                     /locus_tag="IWK54_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9595036.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="IWK54_003000"
     CDS             complement(1040..1396)
                     /locus_tag="IWK54_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9595037.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DACDTH010000185         1435 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDTH010000185 DACDTH010000000
VERSION     DACDTH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.193"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            105..1406
                     /locus_tag="IWK54_003001"
     CDS             105..1406
                     /locus_tag="IWK54_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9595038.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          473 a          287 c          264 g          411 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgtt
//
LOCUS       DACDTH010000186         1417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDTH010000186 DACDTH010000000
VERSION     DACDTH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.194"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWK54_003002"
     CDS             complement(150..1337)
                     /locus_tag="IWK54_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9595039.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DACDTH010000187         1373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDTH010000187 DACDTH010000000
VERSION     DACDTH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.196"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            191..445
                     /locus_tag="IWK54_003003"
     CDS             191..445
                     /locus_tag="IWK54_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9595040.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IWK54_003004"
     CDS             446..763
                     /locus_tag="IWK54_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9595041.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          418 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat ata
//
LOCUS       DACDTH010000188         1356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDTH010000188 DACDTH010000000
VERSION     DACDTH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.197"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            82..>1356
                     /locus_tag="IWK54_003005"
     CDS             82..>1356
                     /locus_tag="IWK54_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9595042.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLIN"
BASE COUNT          461 a          261 c          257 g          377 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaat
//
LOCUS       DACDTH010000189         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDTH010000189 DACDTH010000000
VERSION     DACDTH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.198"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1355
                     /locus_tag="IWK54_003006"
     rRNA            <1..>1355
                     /locus_tag="IWK54_003006"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACDTH010000190         1320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDTH010000190 DACDTH010000000
VERSION     DACDTH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.201"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(31..>1320)
                     /locus_tag="IWK54_003007"
                     /note="aph(2'')-Ia"
     CDS             complement(31..>1320)
                     /locus_tag="IWK54_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP9595043.1"
                     /translation="TEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEI
                     VYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSG
                     FRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGS
                     GYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDE
                     LSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQ
                     NVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDG
                     NNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQD
                     IVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          436 a          226 c          100 g          558 t
ORIGIN      
        1 ttttataata gcttttcata tattatataa tcaatcttta taagtccttt tataaatttc
       61 ttttctacca ttttcgataa attcctgttt aatattttta attccataaa caatagtttc
      121 aataggataa tattcttcaa ctatatcttg atattctttt gctttctcaa tatctatatt
      181 tccatacatt cttaatatat cttctccaaa atttgttcct atttcttctt cactatcttc
      241 aagtaagtat ataaaatcac aatattcatc tataattcca gaatctccaa aatcaattat
      301 tccagttaat ctattattgc catctaacaa tagatgatta caactaaaat cattatggca
      361 taaacacttt ttaccctcaa aaactgttgt tgcatttagt ctttccataa aactttctat
      421 ataatctttt tctatatcag ttaaatcatt ataaatagtt tcacgcaaca atatatactc
      481 ttctaataca ttttgtttat tatcaatagt acattcacta atatctgtat aatctaaacc
      541 gtgcatttgt cttaaaaaac tggcaatatc tcgttttaac aaattttgtt cttcttctga
      601 catagtagaa taaatttctg gtgttaaaaa agttccttta atttctttat aacctagtat
      661 agataattca tcactaatat acgaatattc aatattagga atttttacat tagtttctaa
      721 atttgtattt aaaaaattat atattgcttt ttcttttgca taaccttttt tcttattagt
      781 actaaatttt gttttaaaaa tgtattcatt attaactaaa tatgccacac tatcataacc
      841 actaccgatt atttcaatac tatctacttt gaaattatca aagtaatgct caattaaata
      901 tttcattgcc ttaacatttg tggcattatc atcatatcta tattccatta aataacaatc
      961 ttcttttttg ccctcgtgta attcatgttc tggcaaatct tcaataattc taaaaccaga
     1021 tttttggtat gcccttattg ctcttggatt atttttatga gggtctaaaa taactgcatt
     1081 agcatttctt tcttttttca aaaattcaaa aatcaattta atatatcttg taccaattcc
     1141 tttactccaa taatttggct ctcctataaa ttgatccata ccatagacta tctcatcagt
     1201 ttttggataa tgataatcag tatataactc atcatacatt ttatatattt gtccatatcc
     1261 aataggaaca ttgttatatt caataattac tctaaaaact tcatcttccc aaggctctgt
//
LOCUS       DACDTH010000191         1318 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDTH010000191 DACDTH010000000
VERSION     DACDTH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.202"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            188..391
                     /locus_tag="IWK54_003008"
     CDS             188..391
                     /locus_tag="IWK54_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595044.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            602..1186
                     /locus_tag="IWK54_003009"
     CDS             602..1186
                     /locus_tag="IWK54_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9595045.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          465 a          183 c          252 g          418 t
ORIGIN      
        1 agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc ctcaatcacg
       61 gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt tacaaagtta
      121 taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta gaaagtaggg
      181 attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg atattctaag
      241 taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt tacaggagac
      301 acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa gaaaaaaaag
      361 agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg tctagtcgac
      421 attctttttt tctaattgaa agtgttcata aaatcaccca tttctggaca cttccttttt
      481 ttgctttatt tttcaaaaaa acaattcatt tcatgttcat aaacccataa caatatctat
      541 gttcagttaa actatatgga taaagttatg ttacaataaa attaatgaag gagtgatttt
      601 tttgaaaata ggatacgccc gggtatctac tggtttacaa aatttggatt tacaagaaga
      661 cagcctaaat aaatttggtt gtgaaaaaat ttttactgat catatgagtg gggcaaaaag
      721 taatcgacca ggattagaaa tggccattga ttttgttcgc tctggcgata cacttgtcgt
      781 ttggcgctta gatcgtttag gtagaaatat ggaagatttg atttcaatcg tcaatcgact
      841 gaatgagcgt ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg acaaatcaag
      901 ttctacagga cagctcatgt ttcatttgtt tgcggcattt gcagaatttg agcgtaattt
      961 gatcctggaa cgatccgcag caggacgagc agctgctcgt gcaagaggac ggtttggtgg
     1021 tcgtcctgaa aaattgtctg aacaagattt ggaattactg aagactttag tggatagcgg
     1081 tacacctatt aagactattg cggaacggtg gaacgtctca agaaccacca tttatcgtta
     1141 tttggcaaaa atggaggaga aaaatggaaa gacaaattct cattagtttt tgtcatttaa
     1201 tgctgaaaat ttttgccatt tatctagaaa agtgacaata gtatgagaat tttttgtaag
     1261 tgtctactga taacttattc tcaaatgttg taaggatttt attcatttgt taatatta
//
LOCUS       DACDTH010000192         1314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDTH010000192 DACDTH010000000
VERSION     DACDTH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.203"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IWK54_003010"
     CDS             <1..848
                     /locus_tag="IWK54_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9595046.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          391 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaat
//
LOCUS       DACDTH010000193         1296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDTH010000193 DACDTH010000000
VERSION     DACDTH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.204"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..>1296
                     /locus_tag="IWK54_003011"
     CDS             14..>1296
                     /locus_tag="IWK54_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9595047.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT          402 a          282 c          261 g          351 t
ORIGIN      
        1 ggagtgtttc aagatgtcct attcagagtt taccaaagag ttactagata ttctagacct
       61 aaatcttacc tttcatgaag atgcctttcg caaagagcgc attaatgacg aaacatgttt
      121 tgtttttgat ggcacattga cctatcaacc agaggaatgc ttccattgcc actatcaaaa
      181 taagcaaacg atcattaaat ggggctggaa aaaagtatcc atcttattga atgatgtcag
      241 taactacaag accattgtgc ggattaacaa acagcgcttc aaatgtaaac actgtggaaa
      301 gactttttta gcagaagatt cagttagcga tcgtcgctgc tccattgccc gcagagtcaa
      361 acaagccatt cttgagctat taagtgagcc tatttctatg tctttgattg cacgtatgaa
      421 gcacatttct ccgaccactg tcattcgaat acttcgcagc cttcggccta aaaccgtttc
      481 tttgaaccag ccgttaccag aagtggtgtg tttcgatgaa ttcaaatcgg tgaaaaacgt
      541 ctcgggagcc atgagctttg tcatgatgga cggcaagacc catcaactga ttgatattgt
      601 ggaaaatcgg caactcaatc ctttacgcga ctatttcttg cgctacccta gaaaagtccg
      661 tgaacaagta cgtctagtgg tttcagattt ctatacaccc tatcgcacat tggtgaaaga
      721 gtgttttccg aatgcccgaa tcgtcgccga tcgctttcat attagccaac acatcggtcg
      781 ggcctttacc aaccaccgaa ttcaagtaat gaagaccttt aaaaaagggg atcgtcggtc
      841 gaagcactta aagaaatact ggagactact tcaaaagaat gcgtgggaat tgaatggtca
      901 gcatcgctac tggcgtccgt catttcgaga tcatctgaca gaagcggaga tagtcgatcg
      961 gctattatat tatgatgact ccttaaagcg aggctacgaa gtctaccagg tttttctttc
     1021 cgctatcaga agacaagatg ttcctgaatt cgtcgcgctc ctaaaagaag actacaagga
     1081 gttgccagag cactatcaac cagtgttcac gaccttcaag aaatatcgaa cagaaatcaa
     1141 acgagcgttg cgagttccct attccaacgg ccctatagaa tgtttgaaca accatatcaa
     1201 agtactaaaa cgaattgcct atggctttag aaactttcaa aactaccgag aacgaatctt
     1261 tttatatcga ggaaaatatt tcaagaaaac taagaa
//
LOCUS       DACDTH010000194         1292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDTH010000194 DACDTH010000000
VERSION     DACDTH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.205"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            18..>1292
                     /locus_tag="IWK54_003012"
     CDS             18..>1292
                     /locus_tag="IWK54_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP9595048.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLIN"
BASE COUNT          431 a          245 c          252 g          364 t
ORIGIN      
        1 agaggacgat atgctatatg aatgattcta tcaaaaaaat gctgcgatta atagagaaag
       61 atttgatgat tacagaggtc tcttacgaga cctttcagaa gaaaaagacg ttgatcgtcg
      121 atgctgtttt ctcacctgct ccccatacct gtagaaattg tggttctact gtggtagatg
      181 gaaacgggaa agtaattgta gtgaaaaatg ggaagaagga aacgattgtc cgctttgaac
      241 aatacaatca catgcctttg gttatgcgct taaaaaagca gcgctatacc tgtaaaaact
      301 gtcgaaccca ttggacggct caaagttatt ttgttcaacc cagacattca atcgcgaatc
      361 atgtcagata taaaattgct tctttactga ctgaaaaagt atctttatct tttattgcga
      421 aaaattgtca ggtatctttg accaccgtta ttcgtacgtt gaaagagttt aaaagctact
      481 taccaaagca atctaagagg attctcccca gagtattgat ggttgatgaa tttcgttcgc
      541 acgcttccat agaagataaa atgagcttta tttgcgcaga tggcgaaaca ggacaattaa
      601 ttgatgtctt gcctacgcgt aaattgcctc gattaacaag ctatttctta ggctgcgcta
      661 atcctgaaga agtaaaattt ttagtcacag acatgaacgc cgcctacttc cagcttacca
      721 aacgtattct gccaaatgcg aaagtagtga ttgatcggtt tcatattatc aaacacatga
      781 atcaagcctt taatgagttg cgtatccgtg aaatgaatga acttcgtaaa gtaggacaga
      841 aaagtcaggc agaaaaactg aaaaagaact ggcgcttttt gctaaaaaat cgtgcaaaca
      901 tcaaccatta tgaatacaaa acatggaaaa gttttcgagc accaaaatac ccatttctta
      961 cggaagcgat gatgattgat cgattgcttg gcttttctac gtccctgaag gaggcgtatc
     1021 cctattttca tgaattagtt gaagcctttc gagacaaaga ccctgactta tttttctcct
     1081 tattggcaga acttcccgaa acgttagatg acggctttcg ggaaaagctc caaaaccttc
     1141 tgacctatga agaaggcatc accaacgcga tgatctatcc ttacaccaat ggaaaaatag
     1201 aagcgaagaa tacccatata aagacaatga aacgagtatc ctacggattt aaatcatttg
     1261 agaacatgag aattagaatc tttttgatca at
//
LOCUS       DACDTH010000195         1292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDTH010000195 DACDTH010000000
VERSION     DACDTH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.206"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            164..1036
                     /locus_tag="IWK54_003013"
     CDS             164..1036
                     /locus_tag="IWK54_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9595049.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          406 a          231 c          289 g          366 t
ORIGIN      
        1 tcaattgtat gaaaaattat tagaatttta aaaaatgata aaaattcaaa aagaaaaaat
       61 agtagttatt tacttgtcat gttactaaat aaaaaaataa tttaaatatg atatatagtc
      121 atttatttag tgatttcatt ctaaaataaa gggagaaatg tctatgctat acggtgcaat
      181 tgaagcaggc ggcacaaaat ttgtgtgtgc cattggcgat gaagaaatga cgatcaagga
      241 aagagtgagc tttccgacaa cgacacctga ggaaacaatg cctttagtca ttgacttctt
      301 caaacaatac caagcagatt tagcagggat tgggattggc tcatttgggc ctatcgatat
      361 ccatcgcgat tccgccactt atgggtacat tacctcgacc ccgaaattgg catggcaaaa
      421 ttttgatttc atcggtacca tgaaaaaaga atttccgatc ccgatttctt ggacaacaga
      481 tgtcaatgcg gctgcctatg gtgagtatgt cttcggcagt ggcaaaggat tgagcagtgt
      541 cgtttactat acgatcggta cgggtgttgg cggcggtgcc ttacaagaag ggcgctttat
      601 cgaagggttt agtcatccag aaatggggca tatgctcgtc gtccctcatc caaaagatga
      661 ctttgctggc agctgtccat ttcatggcaa ctgtttagaa gggatggccg ctggacccgc
      721 catcgaaaaa cgcttaggga aaaaaggaca agaggttgca gaagatgatc cttattggga
      781 aatcgaagcc tcctatattg ctcaatgtgc ctacaataca accttgatgt tttctccaga
      841 tgtgatcatt tttggcggtg gtgtgatgaa acaacgccat atgctgcaaa acgtccacga
      901 cgcatttgca aaattggtca atgggtatgt cgaaacacct gagttaagca aatatatcgt
      961 gaccccagca ttagaagaca acgctggaac gctcggttgt ttggcattgg ctcgagaagc
     1021 ggtcttacat tcataaacga aaaaaggctc ttcgacattt agtgttggta gagaaagata
     1081 caactgaaaa tttagtcata accagttagg cagagtgctt aactggtttt tgttttactt
     1141 ggaagatcaa gccttgaatg agataaattc ttgctcgaaa gttatggaaa tttcgatagc
     1201 cgtaggccac ttgtattgtg caatagtttt ttacacgccg tttgtataat taagcgagac
     1261 aaataaaaaa gccatttatg ttacactcaa at
//
LOCUS       DACDTH010000196         1265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDTH010000196 DACDTH010000000
VERSION     DACDTH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.207"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            20..1099
                     /locus_tag="IWK54_003014"
     CDS             20..1099
                     /locus_tag="IWK54_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9595050.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          376 a          301 c          310 g          278 t
ORIGIN      
        1 tagacaagga tgaattcaca tgacgcaaac ccatgataac acagtttcac gcaagggaaa
       61 acatctttcc tactcagaac gttgccaaat cgctatcctg aaaaaggaag gctattccaa
      121 ccgtcaggtt gccaaagtgc tgggacgtgt tccgcaaacc ataaacaatg agattgatcg
      181 cggaaccatc acccagttaa aacgccaaac gcaaaatgga aaagtgtatg acacctacta
      241 ttctgtttat gacgctgatg caggacaggc cttctatgaa aagcaacgac tgaactgtgg
      301 cagacgcccc aaatgggcga acacaaatgc cttcattgat tgggcagatg acaaaatgtt
      361 aaatgaaaaa tggtcgccgg acgtcgtggt cggatttgcc agaaaacacg agctgttcga
      421 ttgtgccatc attcctggca caaccgctct ttatcaatgg attgaccgcg gaatcatgcg
      481 aacaaagaac atggacttgc ttgaaaaact gtcccgtaaa atcaaagcgg tccgcccagg
      541 acaccgtccc aacaagcgtg ttctcgggac gtcaattgaa gaacgcccta aagaagttga
      601 aacacgggaa acgttcggtc attgggaaat cgacacggta gtgggaaaca agaccaagtc
      661 ggatgccgtg ctgctgacgc tggctgaacg gcagacacgc tttgaagtca tcctaaagtt
      721 gaacgggaag gacaccgaat cggtggacca agcggttcaa cagctaagag aacgggctgg
      781 cgctaacttc gatcaattat ttaagaccgt tacatccgac aatggctcgg agttttctgg
      841 gctacacgat gctctgcaag atgtagctga cgtctatttc agccacccct atgcttcttg
      901 ggagcgtggg acgagcgaaa accagcacaa attaattcgc cgtttcgtcc ccaagggaag
      961 cccgattagt gaggtgagcg agaaccagat tttgcggatc cagcgttgga tgaacgatta
     1021 cccgcggaaa attttgggtt atgcgacacc acatgataca ttcgtccagg cattcaagca
     1081 agaacggttg gtggcttgac ccattattcc ttgataacga tcctcactcg ggacatggct
     1141 tcatgtctgg aaagctggcc tggggccaga atcgtctgaa gactatcata cctcagttca
     1201 ttatcaaggc ctcactgatt tagaataacg aggcagttac ggtatcaaac aaatgagtta
     1261 cctgt
//
LOCUS       DACDTH010000197         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDTH010000197 DACDTH010000000
VERSION     DACDTH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.208"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IWK54_003015"
     CDS             200..742
                     /locus_tag="IWK54_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595051.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IWK54_003016"
     CDS             832..>1246
                     /locus_tag="IWK54_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP9595052.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACDTH010000198         1245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDTH010000198 DACDTH010000000
VERSION     DACDTH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.209"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IWK54_003017"
     CDS             <1..1244
                     /locus_tag="IWK54_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9595053.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          410 a          222 c          268 g          345 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaa
//
LOCUS       DACDTH010000199         1231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDTH010000199 DACDTH010000000
VERSION     DACDTH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.210"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..279)
                     /locus_tag="IWK54_003018"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IWK54_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IWK54_003019"
     CDS             complement(545..739)
                     /locus_tag="IWK54_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595054.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          435 a          207 c          168 g          421 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac t
//
LOCUS       DACDTH010000200         1231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDTH010000200 DACDTH010000000
VERSION     DACDTH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.211"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            91..1131
                     /locus_tag="IWK54_003020"
     CDS             91..1131
                     /locus_tag="IWK54_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9595055.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          500 a          177 c          202 g          352 t
ORIGIN      
        1 tgcgtttgca ttttttattt atctttgcta ttttcgaaaa gagtaggtaa ttttacataa
       61 ataaaacaac gtgaaagtag aggatctaca atgactgatt ttaaattttt taaggcggat
      121 agagtttaca acgaattatt ttatcaattt ccaaaagtct ttattgtttc tgacgaatac
      181 aaaaaaatga aagattcaac taagattgcc tatatgcttt taaaagcaag attagagatc
      241 gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt attttactta tacgacaaat
      301 gaactatgta gagtattaaa ctgccaaaaa caaaaagcga tagcaatcaa aaaagagttg
      361 gaatcctttg gtttattatt acaaaagcag atgggattta acaaacagtt agggaaaaat
      421 aatcctaata gactatatct agcagaatta aaagtctcag aaaatgatat ctacttactc
      481 gaaaaatttg atagagagaa tagggaaaac gttgataaat cagagggtat gaaaatcata
      541 cccaccctcg acgaaaaatc agacgctgaa tcccttgggg ctcaagaggg tatgaaaatc
      601 ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa tcataccaga acttaataat
      661 aatatattag acactaatag acacaatata gacactgaaa aagaccgcct acaagatcaa
      721 ttgttgttag acaattttga gacaattatg acaaacgaca gcattgctac gtttgtccct
      781 gaacgagtat taaatttgat aaaaacattt tcttcaagtt acagtgaagc tcaaaaaacc
      841 gtccagacta ttcataatgc aaagaaaaaa gctgaaatag aaagtggtat ttcgatagtt
      901 tttgaagaac tcgatagtta ttatgtcaat gcagaacaag aattatacac gacactgtta
      961 aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata tccagaacct gatttttgtc
     1021 tatgtaaaaa attggtttat cgaaaaacca atagctgcta aagtatcaag tgaaaaacgt
     1081 ttgaattatg aaagctcccc aagcactatt acgaaagact ggttagagtg aaactttact
     1141 gacaaagtga ttgttgcttc atggagtaac attaaaatta aaaatttttt attattctaa
     1201 ttatattacg aatattacaa aataatagct a
//
LOCUS       DACDTH010000201         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDTH010000201 DACDTH010000000
VERSION     DACDTH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.212"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            16..276
                     /locus_tag="IWK54_003021"
     CDS             16..276
                     /locus_tag="IWK54_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595056.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(638..877)
                     /locus_tag="IWK54_003022"
     CDS             complement(638..877)
                     /locus_tag="IWK54_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595057.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          427 a          169 c          221 g          398 t
ORIGIN      
        1 aaggatggat gaaatatgaa taacgaaact atcattttag gaaaaaaata tatttgccaa
       61 cctattggct tgaaacaaaa agtggttgga gaagtaatca gtaaattaga aaattgtgtt
      121 gtgatctgcg tagacgaata tcatgacgtc gatcgcaatg aaatattaga aaaatgcgga
      181 aaagtagtgg caaaatatac tcacgtttat gagttggaaa caacagaata tctatataac
      241 tatagcaaca aatatgaaca tactttagtt tcataaagat ttaaataaag tgccttcaaa
      301 agcgagaaga ccaagctttt gaaggcactt tatttagttg gcagtgattg ttcatgattt
      361 ttgatttatt caaaaggttc gcctagagaa tctgtctctg taacgaggcc tggtggagaa
      421 actcgataaa aacctatact gccagaactg tcctattttc ggatagacaa actagtagtt
      481 ttaaaaagat catccgatcc aatcatttca aaaaatgcaa aactgatatc aggatcattt
      541 agctcaggct tgttatcgta aagcatttgt aaaggttgca tttagttagg agtaaacgcg
      601 aaactatcat cattatttgc tgaagaagat gaagaagcta attcagagtt tgtttgaaat
      661 aaaggagacg tgctgacagt ttctttaccc tgttttttgc agaggtagaa acttttatat
      721 cggaagtttc taccggagaa tttttctcat ttgtagaaat tgttgaatcg atactattcg
      781 aaatttgcgt atctgaaggt atgttctttg atgcagaagt cccacaagca gtaagtaaca
      841 ataaatttga taaactgata aacagaattc ttttcattat gtacacactc cttatagtat
      901 cgtttagcat acacctatgc tcagtattag gcaaaatcaa tgcaaaacta tcaagtcttt
      961 tatagtatct attagtctta gtaatacata tatggagagg gtgatatccc agttcaaacc
     1021 agatacattg gttaccatct gtattaacga ttatgcctct aaacgctttt tttatgaaaa
     1081 aatttataag tgaaaacgta ggtatgatat tatctagagg taattttttt gggtatttaa
     1141 atagttgcta ggaggatagc ttttggattt ttacagtaat tttatcttaa taatagcaat
     1201 attattactt ctaaa
//
LOCUS       DACDTH010000202         1197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDTH010000202 DACDTH010000000
VERSION     DACDTH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.213"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..153
                     /locus_tag="IWK54_003023"
     CDS             <1..153
                     /locus_tag="IWK54_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9595058.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="IWK54_003024"
     CDS             159..575
                     /locus_tag="IWK54_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595059.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="IWK54_003025"
     CDS             681..944
                     /locus_tag="IWK54_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9595060.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1197
                     /locus_tag="IWK54_003026"
     CDS             944..>1197
                     /locus_tag="IWK54_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9595061.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          455 a          154 c          244 g          344 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggac
//
LOCUS       DACDTH010000203         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDTH010000203 DACDTH010000000
VERSION     DACDTH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.215"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWK54_003027"
     CDS             <1..1076
                     /locus_tag="IWK54_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9595062.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACDTH010000204         1180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDTH010000204 DACDTH010000000
VERSION     DACDTH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.216"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            157..942
                     /locus_tag="IWK54_003028"
     CDS             157..942
                     /locus_tag="IWK54_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP9595063.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          456 a          182 c          183 g          359 t
ORIGIN      
        1 ctaaataatt tttgatttaa ttctgcaccc aaaaagatac actcctatct cttatttaaa
       61 gttgctatat accctcttat tttagcatac tcccttttag aaaaagcaaa aaaaaaagaa
      121 aaaatcttat tcttgataac aagctacgtg acgtatatgc ctaacaaaaa tagaactttt
      181 gaatcaatct taaatgaagg tttaaccaca gaaccggcat ccattatttt tgatgaagtt
      241 atcgatgacg ttgaagatag taagtatcat aaaaatttaa aagaactttt ttattttgca
      301 ctcaacaatg gaccaagttt gttcactggt atatcagtac ctgatagtga taatccatta
      361 attatggcaa aaatttactt aacaaaatgg tgtaataaat acatcaggga taggaataac
      421 ccagcattaa aaaaacctct taaaacattt ggcgaaaaag atgctgcttt aactactaga
      481 gtagcagcaa atgctaatat tgatgatcaa aaagtattga atgactactt gcgtggacat
      541 tttttatata tgagcgctga gaacatgaac ggaagtattt tagaggaata tttagccgaa
      601 gtgttagaac ctgaaggttg gatttggtgc gccggctctg tatatcgagc tgttgatttc
      661 tgttatttag gaacctcacc aattctttta caagtgaaaa ataaatacaa tacagaaagc
      721 agttcgagtt cagctataag agtaggaaca acaattagaa aatggaatcg tttaaataag
      781 tcgacaaaaa tttctggttt ggatagccct atccccaatt ggaaagcatt aatagaaatg
      841 actgaagcta gtaaggaact ggcagctaag ttaactgaaa atagttattt agcttatatt
      901 aatgaaaaat ccacacgaga attatggaca ctcgatgact aacaatatcc ttattagtat
      961 ctatccacga tacatggaaa aaattataaa caaacaaaaa aactttgaat ttagaaattt
     1021 ctatcttacc tcagatcctt ataatatctt ttggatttac gaaacaatcc ccaccaaagc
     1081 tttaaagtat aaattgattg taaaaaatcc aattactaaa cttaagaaaa atcaacacta
     1141 ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DACDTH010000205         1175 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDTH010000205 DACDTH010000000
VERSION     DACDTH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.217"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..383
                     /locus_tag="IWK54_003029"
     CDS             <1..383
                     /locus_tag="IWK54_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9595064.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
     gene            complement(367..960)
                     /locus_tag="IWK54_003030"
                     /pseudo
     CDS             complement(367..960)
                     /locus_tag="IWK54_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT          356 a          235 c          207 g          377 t
ORIGIN      
        1 catttagccg tatcaaacaa cctctgacag aggttgagat ttttcatact gatcgtggaa
       61 aagagtttga taaccaagct attgatgaat tattaacaac ttttgacatc aatcgatcat
      121 tgagtcataa aggctgtcct tttgataatg ccgtagctga atcaacttat aagtcgttga
      181 aagtagaatt tgtctatcaa tacacatttg aaaccttaca acaattggat ttggagttat
      241 ttgactatgt aaattggtgg aaccaccttc ggttgcacgg tacacttggc tacgagacac
      301 cggttggtta ccgtaaccag agattggcgc agcgaatcct tgataatgag ctcggatgtg
      361 ctaacgctag cgaggcagtc taactttaac tgttagctcc tgccgaagat cgtcacatcc
      421 gaggaggctc attgtcaagg acaatcggaa tagcatacgg aaagaagttc tgcaccttat
      481 aaaatttgtc aaaaaaactg ttgccattcc accagacact tgcttaaagc gaaaaatttc
      541 ttcaatgtcc atgcctaatt ctttggatat tctcttgtct gaccatccat tttttactaa
      601 tctttggatg acaatttcca ttcctcttat ttcatgggtc cctcttgcac gattgtgtcg
      661 aattgttgaa gccatacgtt cttatatggg tttatcaata actgttaccg gtacaacttt
      721 taagttgagt ctttccttag caacacgata cctatgaagc acatcaacca agatatactt
      781 ctcttctttt tccttgtaat aacaaactaa tggttgagta aagccatctt caatgataga
      841 aagctccaat aagttcattt ccggagacgc tactcgattg ggattataat cgttcgcctg
      901 tattttggta attggaacaa gccgaacgtc catcacaggg aaagttatgt ctttattcat
      961 acgcttgtat accctacttt cttaaaaagt agggtatact caagaatccg attaattatc
     1021 ttctcaattt ggataatata tctcatcagt cttcttgtca ctttatcaac atgattttgc
     1081 tgcaaaatgg gataccgaca gtactggact ggtaatcagg ataaaagcaa aaataattaa
     1141 tttgtatcct aaaaaatcca taaaaattct ctcac
//
LOCUS       DACDTH010000206         1146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDTH010000206 DACDTH010000000
VERSION     DACDTH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.218"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            317..>1146
                     /locus_tag="IWK54_003031"
     CDS             317..>1146
                     /locus_tag="IWK54_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9595065.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          404 a          156 c          191 g          395 t
ORIGIN      
        1 attttaggtt tcatattata taaattaata atatactacc atttgttggt agtataattt
       61 aaaggtaata agttattctt ttattactat gtttgggttt tatagcaaag aaagtttaat
      121 aagagtttgg acctagcaaa caatattaat gaggtcatta aatcttatta tctttagtga
      181 tataacgaaa tatctacaca gacaaaatga tagcgtaaaa tattttgtgt aaatagatat
      241 ttagggtaga aaaaaagaaa gtccattctg taaaattaaa gttaccacaa ccgaaatttt
      301 ataggaggac tttctcatgc ccaattttac tacagaaatt atggaaacac taatcaataa
      361 aggtgattta gatgaattat ttcgatgtca tttagaatta gctgttaatt cattattgca
      421 ggctgaatta acagcttttc ttgattatga aaaatatgat cgtgctgggt tcaattcagg
      481 taattcccgt aatgggaatt attcacgttc attcagaaca gagtatggag aattaaattt
      541 ggtaattcct agagatagaa atggtaagtt ttctcaacaa actttacctg cttataaaag
      601 aaccaatgac tctttagaaa ccactattat tcagctattt caaaaaggaa ttactatgtc
      661 tgaaatctct gaattgattg aaaaaatgta cgggcatcac tatacgccac aaaccatttc
      721 aaatatgact aaaatagttt ctgaagatat tattgccttt aaagaaaggt ccttagaatc
      781 cagatactct gtcattttta tggatgctac tcacattcct ttaaaaagac aaactgtttc
      841 aaaagaagca gtttatattg taattggcat tcgattagat ggaaccaaag aggttcttgg
      901 gtttagtctt gctccaactg aatctgctta tgtttggaaa gagattcttc aagacctaaa
      961 agaccgtggt ttaaaagagg ttttattagt cgtaactgat ggtttaagtg gcattaacga
     1021 tagtattcat agtgtttatc caaatgctca atttcaacaa tgttgtgtcc atatatctag
     1081 aaatatcgct cataaggttc gtgttagtga tcgacaagaa atctgtagcg atttcaaatt
     1141 agttta
//
LOCUS       DACDTH010000207         1144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDTH010000207 DACDTH010000000
VERSION     DACDTH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.219"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..634)
                     /locus_tag="IWK54_003032"
                     /pseudo
     CDS             complement(1..634)
                     /locus_tag="IWK54_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          362 a          191 c          197 g          394 t
ORIGIN      
        1 aggtaaaaaa taagtctgat tccttgtctc taaaggcttc ggtcagctca tggaaaaaag
       61 tataggcgtc ctttaaaggc gttgaaaatg ccagcaatcg atcaatcatc attgcttcag
      121 tgagtagcgg gtatttaggt gcacgaaaac tgcgccatgt tttgtaatca tagtgattga
      181 ttttttgacg gttttttaga agaaagcgcc agttcttttt cactttctcg gcttcccgtc
      241 gttgccctaa ctggtgtaat cttttcattt cttgtacacg aaaatcattg aacgcctgat
      301 tcaaatgttt gatgatatga aaacaatcaa tgacgacttt cgcatttggt aagacacgtt
      361 ttgtcagttg gaagtaggcg gcgttcatat ctgtcactaa atattccacc gcttcaggat
      421 ttgcacagcg actaaaatag tgtgttaatc gagacaattt tcgtgtaggc aaaatatcaa
      481 tgagttttcc agtctcgcca tccgcacaaa taaagctcat tttatccaca attgaagcgt
      541 gcgaacgaaa ttcatccacc atcaatactt ttggcagggt cctttttgac ggcttcggaa
      601 gatagttttt caactctttc aatgtacgaa tcatatacct aaaataacaa tgggactgaa
      661 gaatcaattt tgattcatca gtcccataaa ttatagagcc tatattttgc gaattgcgct
      721 acggtttctt cttttctttt gtctgtaata tgaatatata gatccataat gatttgaata
      781 gaagagtggc ctaaacgctc ttgcacatct ttaatatttg cactagcttg ctaaaagcaa
      841 gctagcatgt gtgtgtctaa gaccatgaat agtaattcgt ttaagattgt ttttcctgat
      901 gattacatct aaccattttc gagatttaga taattgaagg tgttcatttc tttctggtaa
      961 aaatttaaat atataactac attttctcta ctaactttga aacactactt ttgtaatata
     1021 aaaaatattt tattaataat aaaattaatc acattttata tagtgagtaa tctagtgttt
     1081 taaaaaaata aatgatacta tatgtaatat aaaaaaacat gaggtggtat atgaatttga
     1141 aaaa
//
LOCUS       DACDTH010000208         1143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDTH010000208 DACDTH010000000
VERSION     DACDTH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.220"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWK54_003033"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWK54_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9595066.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DACDTH010000209         1120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDTH010000209 DACDTH010000000
VERSION     DACDTH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.221"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..785)
                     /locus_tag="IWK54_003034"
     CDS             complement(<1..785)
                     /locus_tag="IWK54_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595067.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRI"
BASE COUNT          366 a          204 c          113 g          437 t
ORIGIN      
        1 tctatccttt tctctttatc ttcaccttca atctggggag catatagtcc aatttcctcc
       61 caataatatg aatctctttc aattcccatg atggtagctt tggctaattt caaatcaaat
      121 aacgatttta gaattcttag tgtctcaata aagtcttctc tcttcaactc tttaatccat
      181 tgtgaatttt ctggattatc taaaatttca agttcatctt cattataagt atatttcaaa
      241 tttgacttgc ttaactcata gataaatgaa gtttcttgac tcataattct tttaagtact
      301 tgactataag tttcgtttga ctttgcccat ttcgctaatt ctaccactgt attcactaat
      361 aaatcgaaat aatgatgttc tcgttcaata gtctcagcat tttgaaaatg aaaatcacct
      421 ttataaactc cattcttttc cactgtttta ttaatatttt ttaaaaacca gaatgagttt
      481 gcaaatttag tcgtactttt tcgattaact aagttaataa ctcttccatg taataataag
      541 acaaaaatac ataaattttt agcttcctta ttattagata ctaattgatc aatcataggg
      601 agccattttt tgattttcac taattgattg ctcataaatc tttcactgga cttttgttca
      661 atcttaaatt gcaaaacttc taactcataa ttcccatctt ctgatttatt tttgggaaca
      721 actatcaaac ttttataaag tttatccaca tcaaaattta tcttttttgc ataatttgta
      781 gccattaata catctcctta ttcataataa atttcatatg acgctcctct caaaatttac
      841 cggtagacta gaaaaaagaa taaaagatac gtcaattcac actttattct taaaaaaaaa
      901 ctggtctctc taccggtaat atcgattgag tccgccttta tcgactttat ccttttttta
      961 tattaaattt tttcaccggt agacagggag agaaagaata aactaaaaag cccttaattc
     1021 ttaatgtttt cacctcctta ccgttcctac cgctaaaatt taatataact aaattctata
     1081 acattagaag aaattttaaa tacctttgat aaaaaatatt
//
LOCUS       DACDTH010000210         1114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDTH010000210 DACDTH010000000
VERSION     DACDTH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.222"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..897)
                     /gene="vanY"
                     /locus_tag="IWK54_003035"
     CDS             complement(<1..897)
                     /gene="vanY"
                     /locus_tag="IWK54_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9595068.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          399 t
ORIGIN      
        1 agtatgtgtt gatccgggaa acactgtcac aattacaccg tctatattgt ttcctgatat
       61 ctcataacga agattagtcg gcacatgaat ggtggtattt ttagtaacag gataataaaa
      121 gatctcatat ttttccccat ttacactaac agaaatggtt ttttcttctt ttaggtaatc
      181 catatattcc tcgagaacga aattcttttc tttcataatc gcactatgtg gtaaaccaac
      241 atagcgaata tgccatggtt catattgaat tcctgttaac tctgttttgt cctctggata
      301 acgtaaaatg aacccgtatt tccaagcatt ttcttctatc cactttcctt caggggctcg
      361 ttccattttc gtcaagcttg atcctacatc tagtgataaa cctgaattat gctcactata
      421 acctgctggt aaggcatact cagcccccat ttcttggtaa agcacacttt gctcatcaaa
      481 gtctcgatag ccactattaa taataaaatg actaacgcca ccctttacag catcattgac
      541 catctctgaa aatttttgtg ctatttcttt tgacatataa atattactat caagcaaccc
      601 gtatccattt attaattcgt catgtttaga taaattcacg atatctgact tcacactttc
      661 ttggcgaaca ggatatttac tattgattaa tagcagattt ccttgataaa cctgttcttc
      721 tgtaattgtt ttctcttggg tattttcaga agtcccacta ttttctaaat gttctttggg
      781 attttgatca taattttgaa attcgacttt ttcctgagaa aacagtgctt cattaacgta
      841 gtcataacct aagtatatta agaataacaa taacaataaa aaaaacaact tcttcatttt
      901 cagtctcctc cttaactata tagttggaag tatgaaatat ttctagaatc ttccccttaa
      961 acggcacaat ctcgtttgag attgtgccgt tttgccccgt aaattacagt caattatttc
     1021 gccctactgg atttactatt atcaccaatg tagatgaatt ttcataagtg atacccatgc
     1081 tgaatttaac gaggctgaac tgcctgttga caat
//
LOCUS       DACDTH010000211         1107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDTH010000211 DACDTH010000000
VERSION     DACDTH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.223"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(24..113)
                     /locus_tag="IWK54_003036"
     tRNA            complement(24..113)
                     /locus_tag="IWK54_003036"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(75..77),aa:Ser,seq:tga)
     gene            complement(137..210)
                     /locus_tag="IWK54_003037"
     tRNA            complement(137..210)
                     /locus_tag="IWK54_003037"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(174..176),aa:Ile,seq:cat)
     gene            complement(224..297)
                     /locus_tag="IWK54_003038"
     tRNA            complement(224..297)
                     /locus_tag="IWK54_003038"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:Met,seq:cat)
     gene            complement(325..397)
                     /locus_tag="IWK54_003039"
     tRNA            complement(325..397)
                     /locus_tag="IWK54_003039"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Ala,seq:tgc)
     gene            complement(417..490)
                     /locus_tag="IWK54_003040"
     tRNA            complement(417..490)
                     /locus_tag="IWK54_003040"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(454..456),aa:Pro,seq:tgg)
     gene            complement(500..573)
                     /locus_tag="IWK54_003041"
     tRNA            complement(500..573)
                     /locus_tag="IWK54_003041"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(537..539),aa:Arg,seq:acg)
     gene            complement(584..669)
                     /locus_tag="IWK54_003042"
     tRNA            complement(584..669)
                     /locus_tag="IWK54_003042"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(633..635),aa:Leu,seq:taa)
     gene            complement(686..757)
                     /locus_tag="IWK54_003043"
     tRNA            complement(686..757)
                     /locus_tag="IWK54_003043"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(723..725),aa:Gly,seq:gcc)
     gene            complement(769..841)
                     /locus_tag="IWK54_003044"
     tRNA            complement(769..841)
                     /locus_tag="IWK54_003044"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Thr,seq:tgt)
     gene            complement(859..940)
                     /locus_tag="IWK54_003045"
     tRNA            complement(859..940)
                     /locus_tag="IWK54_003045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(904..906),aa:Leu,seq:tag)
     gene            complement(948..1020)
                     /locus_tag="IWK54_003046"
     tRNA            complement(948..1020)
                     /locus_tag="IWK54_003046"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(985..987),aa:Lys,seq:ttt)
     gene            complement(1024..1096)
                     /locus_tag="IWK54_003047"
     tRNA            complement(1024..1096)
                     /locus_tag="IWK54_003047"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1061..1063),aa:Val,seq:tac)
BASE COUNT          289 a          315 c          261 g          242 t
ORIGIN      
        1 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
       61 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      121 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      181 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      241 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      301 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      361 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      421 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
      481 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
      541 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
      601 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
      661 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
      721 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
      781 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
      841 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
      901 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
      961 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1021 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1081 gctgagctaa acctccatgg ataactt
//
LOCUS       DACDTH010000212         1068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDTH010000212 DACDTH010000000
VERSION     DACDTH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.224"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            287..694
                     /locus_tag="IWK54_003048"
     CDS             287..694
                     /locus_tag="IWK54_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595069.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..>1068
                     /locus_tag="IWK54_003049"
     CDS             708..>1068
                     /locus_tag="IWK54_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595070.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDY"
BASE COUNT          398 a          135 c          182 g          353 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactata
//
LOCUS       DACDTH010000213         1063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDTH010000213 DACDTH010000000
VERSION     DACDTH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.225"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(38..994)
                     /locus_tag="IWK54_003050"
     CDS             complement(38..994)
                     /locus_tag="IWK54_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595071.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caa
//
LOCUS       DACDTH010000214         1055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDTH010000214 DACDTH010000000
VERSION     DACDTH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.226"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            73..690
                     /locus_tag="IWK54_003051"
     CDS             73..690
                     /locus_tag="IWK54_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595072.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          383 a          140 c          180 g          352 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa
      241 ggtcgtttag ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc
      301 ctatatgact atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa
      361 gtaaaagttg gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc
      421 tatacagtag aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga
      481 acagttgtga aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct
      541 attaaaggta cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg
      601 attctatctt tattaattag ttcaataact tttatattct tatttacacg ttatttaata
      661 agatgctgcg gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt
      721 tcatagcaat ggagaaaata cattagcaat atataaaaga aggtatcaag agtcacagtg
      781 aatttattcc gaaaagtgaa aagagcaaat ataaaattta atgaaaatat agaactaaat
      841 aggaaaacgt agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa
      901 aattatataa actaatttag tgttttgttt gtaattccta attttgctcc acttttagat
      961 aatttttagg tctatttaac tcatcagaca ttctatgctc atttttctcc aaatttgttt
     1021 caactctttc gtacttaatt ttgcgtattt gaaaa
//
LOCUS       DACDTH010000215         1051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDTH010000215 DACDTH010000000
VERSION     DACDTH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.227"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IWK54_003052"
     CDS             6..980
                     /locus_tag="IWK54_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9595073.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DACDTH010000216         1025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDTH010000216 DACDTH010000000
VERSION     DACDTH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.228"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            225..518
                     /locus_tag="IWK54_003053"
     CDS             225..518
                     /locus_tag="IWK54_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9595074.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT          382 a          147 c          183 g          313 t
ORIGIN      
        1 attcaagggt tcttttttct tagcatgtaa tttgaacacc ctgatccagt tttttcttta
       61 attattggta ggggagaata atggattaga ataaatctac ctataagtag tagtaaaaaa
      121 aagagcacta tgataatatc tttcttaaga gtaaccgctt taataaaaaa tccagaatat
      181 tctaacataa atggagtatt cttagaatca aaaggagaaa gaagatggtt tatacttata
      241 cggagcaaga aaagatgaaa gttttagaca cattcatgaa tgatggaaaa gtaaaacaag
      301 taccaagttc aacaaaaaag aaatacatcc tattgaaaga agtcttgaaa cgctttgacc
      361 atggagtgac ttatacagaa acagaggtta attcgatact gctgaacgta ttttctagtg
      421 gcgattatgt cgaacaaaga cgttatctaa tcacatttgg ctttttcaaa agaagttcag
      481 acggtcgggc atatcagatg atgggaattg aaaattagaa gttttaattt atactcattg
      541 aaaaaagttc aagtatgcga tattggttct acaccgcata cttgaacttt ttttgtcatc
      601 tgtatctata atacaaagcc aagaacgtta ttcaaaatat ggatagctat gataaaagct
      661 aatgagcgat tcgcagttaa ataagcaatc gtcagtaaaa tcgttccagg agcgtacatg
      721 aaaaaatcaa ttggatgcgt tacatgaata aagataattc tcttttattt gtacgaataa
      781 ataccaaaac gtacctccta aaattagatt ttggacagat gccctttaaa aagataacaa
      841 gatttcgttc ttgaaacaat atatatgctt ttattccaat gaggttgtga aaacagtatt
      901 ttgtcctgag cgttagaaaa ttgaaaatgg ggaatagcaa ctcgtacttt accatttttt
      961 aagtgaatac aagatcagct aattgaacac cgtttattcg tgatgaggga cacaacactg
     1021 cagtc
//
LOCUS       DACDTH010000217          995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDTH010000217 DACDTH010000000
VERSION     DACDTH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.229"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(24..911)
                     /locus_tag="IWK54_003054"
     CDS             complement(24..911)
                     /locus_tag="IWK54_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9595075.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          287 a          207 c          179 g          322 t
ORIGIN      
        1 atttctttgc ctttttatct aaatcaaacg tttagttctt gaccaactaa taaagaataa
       61 ctggacatag acatgccatt tttcgccatt agatcatcaa cagacatgcc aaatttttgt
      121 gcaatatccc atagcgtatc acctgacacg actgtatatg tctgaccatt atttgtattt
      181 acatctgttg ttgaagatga tgacgttgaa gtcgtcgtag tagtcgttgt tgttgtactt
      241 gtggttgttg tagccgaacc tgttgatggc gtatcaaaac gggttaaatt ataagtatta
      301 atcaatgagt tcaatttggc cccataattt ggatcggtag cataacgacc tgtcaagtac
      361 gctgttgcat cataaaaact tgttgtattg cttttccaca cgccagcata atgataatcg
      421 cccgtggaaa aactggttga acgaagaaca ttcgcatggt cttggaagga ttcccaataa
      481 gaagaatatt ttcggaatgg ctcattcatc gttacccatt gtccgtttac atattctttg
      541 gttgccatat aaacgacctg tccgttatag cttcctttca caccgaatag attataattt
      601 ggcgcactag ctaatccgga agtgccatat gcactttcca acaacgcttg agcgatcatg
      661 actgaagcat acaagtcatt acttgcagca acttctgacg cagaccagcc gatttgatcg
      721 ataaacgctt gaggatcagc gactgtttga gatccttgtt catctgcatg aacatgttgc
      781 ggtaccgctg taaccatcat cggcccaact aaaagactcg ttccaattaa aggagcaatc
      841 gcttttttat tgatcaccat ttttctttta ggtctatggt gccgggcact gcgggtcttc
      901 agctcttgca ttcaaattcc tcctcaaaat aaaaaaactt aataaaaagt tctcttctta
      961 aaacaaactt aattaattaa attatacttt taatt
//
LOCUS       DACDTH010000218          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDTH010000218 DACDTH010000000
VERSION     DACDTH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.230"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>994)
                     /locus_tag="IWK54_003055"
     rRNA            complement(<1..>994)
                     /locus_tag="IWK54_003055"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACDTH010000219          988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDTH010000219 DACDTH010000000
VERSION     DACDTH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.231"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..704
                     /locus_tag="IWK54_003056"
     CDS             <1..704
                     /locus_tag="IWK54_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9595076.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          335 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataa
//
LOCUS       DACDTH010000220          872 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDTH010000220 DACDTH010000000
VERSION     DACDTH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.232"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>872
                     /locus_tag="IWK54_003057"
     rRNA            <1..>872
                     /locus_tag="IWK54_003057"
                     /product="23S ribosomal RNA"
BASE COUNT          263 a          174 c          260 g          175 t
ORIGIN      
        1 agggcgcacg gtggatgcct tggcactagg agccgatgaa ggacgggact aacaccgata
       61 tgctttgggg agctgtacgt aagctatgat ccagagattt ccgaatgggg gaacccaaca
      121 tctttaatag gatgttacga ttgtgtgaat acatagcaca ttcgaggtag acgcagagaa
      181 ctgaaacatc taagtacctg caggaagaga aagaaaattc gattccctga gtagcggcga
      241 gcgaaacggg aaaagcccaa accagcaagc ttgcttgttg gggttgtagg actccaatat
      301 ggtagttctt tcagatagtc gaatgacttg gaaaagtcag tcaaagaggg taaaaacccc
      361 gtagacgaaa tgtggaagac acctaggagg atcctgagta cggcggaaca cgagaaattc
      421 cgtcggaatc cgggaggacc atctcccaag gctaaatact ccctagtgac cgatagtgaa
      481 ccagtaccgt gagggaaagg tgaaaagcac cccggaaggg gagtgaaata gaacctgaaa
      541 ccgtgtgcct acaacaagtc aaagcccgtt aatgggtgat ggcgtgcctt ttgtagaatg
      601 aaccggcgag ttacgattgc atgcgaggtt aagttgaaga gacggagccg cagcgaaagc
      661 gagtctgaat agggcgtttg agtatgtagt cgtagacccg aaaccatgtg atctacccat
      721 gtccaggttg aaggtgcggt aaaacgcact ggaggaccga acccacgtac gttgaaaagt
      781 gcggggatga ggtgtgggta gcggagaaat tccaaacgaa cttggagata gctggttctc
      841 tccgaaatag ctttagggct agcctcggaa tt
//
LOCUS       DACDTH010000221          858 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDTH010000221 DACDTH010000000
VERSION     DACDTH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.233"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..543
                     /locus_tag="IWK54_003058"
     CDS             <1..543
                     /locus_tag="IWK54_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9595077.1"
                     /translation="ALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDK
                     NQLVSEKTNLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKN
                     KFNEEQSTINSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAK
                     NDVEDTANKAQQIVNEHTIQ"
BASE COUNT          382 a          106 c          119 g          251 t
ORIGIN      
        1 gctctagaaa ataaagagca aaaaattagt attttaaatt cacaattgtc ttcaaacaac
       61 caaactcttg aacaactaag aactaatatt gaagtttcca aacaaaaagt ggcatcttta
      121 gaaaacgaca agaatcaatt agtatcagag aaaactaatt tagaaaatca attgtcagca
      181 aaagactctg aattgcaagg aaaacaaaat gaaattaatg cgaaaataga cgaaattaac
      241 caaaaagtag ctgaaataaa tcgattaaat agcgattgga ataatcgttt acaaaatgaa
      301 aagaacaaat ttaatgaaga acaatctact attaatagtt taaaccagca aattaatcaa
      361 atgttgagtg aaaaaaataa cattcaaaat caactgcaag ctactcagca aaagctagaa
      421 gaagaaaagt caaaaaatag tagtttgaat aattacattg aaaagcttca aaaagctaaa
      481 aatgatgtag aggataccgc aaataaagca caacaaattg taaatgaaca tacaatccaa
      541 tagatatagt tttaatttaa caaatggtat gtttggagat aatgagatag aatataaatg
      601 tcataatatt atacaatcta aacaccgttt ccttttctat ttttgatgac atcagtatat
      661 agctactcga aagagtagtc tttttattat tctcgcttat tattgatatg aaaatttcga
      721 tttgacaaaa ggtaccgaat aattttgtaa tatgaattat tatcaagaag taattatgat
      781 gataacatat ggaaactagt tagtaataat aacaggtgat taaacttttg aacgacttgt
      841 aattctatat tttttctt
//
LOCUS       DACDTH010000222          838 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDTH010000222 DACDTH010000000
VERSION     DACDTH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.234"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..94
                     /locus_tag="IWK54_003059"
     CDS             <1..94
                     /locus_tag="IWK54_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP9595078.1"
                     /translation="KKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            91..498
                     /locus_tag="IWK54_003060"
     CDS             91..498
                     /locus_tag="IWK54_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9595079.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            699..>838
                     /locus_tag="IWK54_003061"
     CDS             699..>838
                     /locus_tag="IWK54_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP9595080.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AIT"
BASE COUNT          318 a          119 c          151 g          250 t
ORIGIN      
        1 aaaaaaacaa gccttatctg atgaaattga tttagcaata tctcaagcat taactgaata
       61 tgatactact tttagagagc ttgtcgataa atgagtattc attacctaga agccaaagat
      121 attataaaaa tgaatgtaat ccaaataaaa aaatactctc ctaatgaacc ccttggtgta
      181 aaggatgcga atgctttaga aatgtgtgta gggcaacttc gagcctcagc ttttggtgaa
      241 gaggtttatc cttcgattta tgaaaaagca gctatcctac ttatacaatt gataaagaaa
      301 catccttttc acaatgcaaa taaacgtact gctttcttag ctacttttgt ctttttgaaa
      361 atcaatggac agcttttagc tattgatcaa aaagatgctg taaatttagc ggtatatatt
      421 gctacgtatg ataaggattt cgatcaacta aaatatgaag tgatcaacac tataaagaca
      481 catacgcagc ccatttaagg agttactgta tgtgtcttta tagggttatt agctgctata
      541 gcgaaataat tgaagtaaat tacaaatagc tataattcaa atccaactat atagaaaaaa
      601 caatctttct atagagttag acatgagagg gactatgctt gatttaaaat caacaagcgt
      661 atatactgta tatacaaaga aaaaggaggg agaagactat ggcaattaaa gaaagaaaat
      721 ttagggaagt ggggaactca gtagtattaa ctatttcaaa ggagtttctt aaagaaaatg
      781 ggttaaatcc cggagatacg gtttttttag atgaagaaaa acttcgagcg gcaattac
//
LOCUS       DACDTH010000223          813 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDTH010000223 DACDTH010000000
VERSION     DACDTH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.235"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            533..>813
                     /locus_tag="IWK54_003062"
     CDS             533..>813
                     /locus_tag="IWK54_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9595081.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
BASE COUNT          270 a          130 c          171 g          242 t
ORIGIN      
        1 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcgtaa attagagcgt
       61 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
      121 tgccgaggat aaggagttga cggctaaacc actaatccct gaagctgaca tttgctaaaa
      181 aatggcaagt ccattttttg gccaacctag cgttagcgaa gtttgggggc gcccttttca
      241 aagggtgcgg ttttattttt tggatatttg catagctaaa gagaagtgct attattgagt
      301 tatcaaataa ataaactgaa aaaagagcaa aaaaataaag tcgagcaacg ctggacacgt
      361 tctcgacctt tacggaaact aatcgcacta gctccgtaac ttcttaatca ttatacgaat
      421 atttttagat ttcttcaaga ctagagattt tttggtaatg aatttttaag agttacgtga
      481 acttttgaaa attctttcca gaagtctctt tttctttgag gaggaacaaa atttgagtaa
      541 aaaaggcacg atcataaatg agtgggaaaa attaacagcc ccctctaaca ggcggttaaa
      601 gtatcaaagt ttgacccttc ccttaaaaat gcagtggtca attgaccgaa ttaccatagt
      661 cggaaaactg aaagaaaata tttattatca cactccaaat gatgtgttga ttttaaattt
      721 tgaacagctt atgcgcctaa atgaaggtaa tggttattta agatctgtta gtaataatgg
      781 ttggcagttg ttagaccaac atgaagagaa tat
//
LOCUS       DACDTH010000224          811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDTH010000224 DACDTH010000000
VERSION     DACDTH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.236"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IWK54_003063"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IWK54_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9595082.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACDTH010000225          768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDTH010000225 DACDTH010000000
VERSION     DACDTH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.237"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            261..542
                     /locus_tag="IWK54_003064"
                     /pseudo
     CDS             261..542
                     /locus_tag="IWK54_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            616..>768
                     /locus_tag="IWK54_003065"
     CDS             616..>768
                     /locus_tag="IWK54_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9595083.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          273 a           96 c          136 g          263 t
ORIGIN      
        1 aaaagttttt gatcctttat tttcaagtct gatttggcag cttttcggca gctcatttat
       61 ttatggtcag aaatagatgc tgaaatttta atctattagc aaatagtcca gaacgtatga
      121 agaggagtcg gatatcattt gtcattaaca ccataaatat ttataaaatt cgtgttcacc
      181 tacaattaaa tttaatatat aatattcgtt gtaagggctt ataatttttt gttaatgaac
      241 aagataggaa gaaacattta atggacagtt ttcagagatt gtcactagta aatcaatttg
      301 agttattggc taaatttgag gaccaaaaca gatcttatta tgaaagaaaa atcgaaatat
      361 tgagagaagg ttacgagtat cattatgatg atgaaatttg gagtgatctt tctgagccat
      421 ttccgaaaga agattctcga tttgtattag atgtattgaa catgtatcga gatatcaatt
      481 ttagtaaatc aaagctaaac ttagatgaaa accaaattac taattcatac tatactcatt
      541 tcggttctgt tgcaaagttt tccaaaaaat ctattttagt gtaaaattga gaaaaaagac
      601 agagaggaca gagtaatgaa tcattttaaa ggcaaacaat tcaaaaaaga cgtcattatt
      661 gtcgctgttg gttactacct gcgttacaat ctaagctatc gtgaagttca ggaattgtta
      721 tatgatcgtg gaataaatgt ttgtcatact acgatttatc gttgggta
//
LOCUS       DACDTH010000226          764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDTH010000226 DACDTH010000000
VERSION     DACDTH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.238"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(109..>764)
                     /locus_tag="IWK54_003066"
     CDS             complement(109..>764)
                     /locus_tag="IWK54_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP9595084.1"
                     /translation="GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPL
                     EAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV
                     KLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          213 a          175 c          146 g          230 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccga
//
LOCUS       DACDTH010000227          755 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDTH010000227 DACDTH010000000
VERSION     DACDTH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.239"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            330..728
                     /gene="spxA"
                     /locus_tag="IWK54_003067"
     CDS             330..728
                     /gene="spxA"
                     /locus_tag="IWK54_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9595085.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          278 a          106 c          135 g          236 t
ORIGIN      
        1 tactattaaa gaaataaaaa acataaaaaa tcttaagaaa tcggtttcat ttagcttttg
       61 agataaaata atagtacagt tttatacgct gtttcatatg tttagaaaat ttgaattaga
      121 tttgaaaact ctaattttta aatgataaaa aaagcgagaa tgctggtttt actgtatatt
      181 aaccttagat aactttcatt tttttaaaaa aattatgaaa aaaaggtagc tatttaaaag
      241 ttgattatgt ataatagaaa cagatgggag attattatga tcttctgtta caaaaaagtg
      301 attcttatca cgaaaaggag tggtgcacgt tgttaacatt atatacttcc ccaagttgta
      361 cttcctgcag aaaggctcgt gcgtggttgc aagaacatca gattccattt gttgaacgta
      421 atattttttc agaaccattg aacagttctg aattaaaagc gattttacaa atgacagaag
      481 acggaacaga agaaatcatc tcgacacgtt ccaaagtatt ccaaaaactg aacatggatt
      541 tagatgatct gccgttacaa gaattattgg aacttgtgca aaacaatcct ggattgcttc
      601 gccgtccaat catgattgat gataaacgtc tgcaagttgg ttttaatgaa gatgaaattc
      661 gtcggttcct accgcgcgat gtacgacaat tagaattacg tcaagcacaa ttgatggctg
      721 gattatagaa ggataatacg aaacttggaa aagac
//
LOCUS       DACDTH010000228          714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDTH010000228 DACDTH010000000
VERSION     DACDTH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.240"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..448)
                     /locus_tag="IWK54_003068"
     CDS             complement(<1..448)
                     /locus_tag="IWK54_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9595086.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          216 a          124 c           95 g          279 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aacc
//
LOCUS       DACDTH010000229          698 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDTH010000229 DACDTH010000000
VERSION     DACDTH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.241"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..697)
                     /locus_tag="IWK54_003069"
     CDS             complement(<1..697)
                     /locus_tag="IWK54_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9595087.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKF"
BASE COUNT          241 a          116 c           72 g          269 t
ORIGIN      
        1 gaaattttct tgtaggaact cttccaaaat aatctaaatg aatttctata attttatcat
       61 cgtatctcaa tatatatgtt gctaaatcat tactgttaat ttcaagattc gaatatttac
      121 caaactgttt ttttactgat ttagggaaac caaataggtt tataatataa tccaattcat
      181 gtatacagtc taattctact cctcccccca tagatttaaa ggcagaataa ttatttctat
      241 aatcagtacc ttttctccaa tcaggaagat aagtagaaca aatagctctt acagaatata
      301 cttttttact ttgaatacta ttacgaacaa cttccatgat atttttatac ctcagtggag
      361 cagcaacata aatatttttg taatcataat tatattgagt aagatcaata ctctcagaaa
      421 aagcaggttt ttctaaaaat atgtaatcaa ctttttcaac aattacattt agtgtttcca
      481 gatgcttaaa agtcggattt gtaataaaag ctatatcata atgtgtatcc aaatcgtctc
      541 tattttgaat atacgttatt tcttcaaact ctttttttcc actgctacta taagctgtta
      601 tatcaaaatt aatagaattc ttttttccat agcttattag attttttaga tgtctctttc
      661 caattgaccc taacccaaaa aaaattactt tcatcatt
//
LOCUS       DACDTH010000230          695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDTH010000230 DACDTH010000000
VERSION     DACDTH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.242"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>695
                     /locus_tag="IWK54_003070"
     CDS             <1..>695
                     /locus_tag="IWK54_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9595088.1"
                     /translation="QGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTP
                     DVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHE
                     REKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLN
                     QWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLR
                     RDGLLKSMDFNSV"
BASE COUNT          236 a          118 c          141 g          200 t
ORIGIN      
        1 gcaaggaaaa tcagccttag aatattatca acagtataag aaaaacaaat caaactgtgg
       61 tagacgtccg cttgttttac ccgaggaaca atcagaatat attcaaagaa aggttgttca
      121 aggatggaca cccgatgtga ttgttggtcg tgcagcgttt cctattagtt gttctgctcg
      181 taccatttat cgtatgttca aaaaggggct atttgattct tctgacttac cgatgaaagg
      241 caagcgtaaa ccgaatggac atcaagaaag acgtggaaaa caaactttcc gccgctctat
      301 tcatgaacgt gaaaaggatt atagccaatt ctcaaatgag tttggtcacc ttgaaggtga
      361 cactatcgta ggtctgaaac ataaaagtgc tgtaattacc ttagttgaac gattatcaaa
      421 agttatcatc acattgaaac cgtgtggtag acaagcgatt gatattgaaa aaaaattaaa
      481 tcaatggttt gaatctgtac cgaaaaacct attcaaatcc atcacttttg attgtggaaa
      541 ggaattttca aattggaaac agatcagtaa tgtcaatgat attgccattt atttcgctga
      601 tccaggaacg ccgtctcaaa gaggcctaaa cgagaattct aacggattgt tacgtagaga
      661 tggtttattg aaatctatgg atttcaattc agtag
//
LOCUS       DACDTH010000231          688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDTH010000231 DACDTH010000000
VERSION     DACDTH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.243"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            124..327
                     /locus_tag="IWK54_003071"
     CDS             124..327
                     /locus_tag="IWK54_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9595089.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            327..677
                     /locus_tag="IWK54_003072"
     CDS             327..677
                     /locus_tag="IWK54_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9595090.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          287 a           69 c          115 g          217 t
ORIGIN      
        1 tcaatcattg ttatataata aaatgttata taataaaatt actaatgata tttaatatta
       61 caagcaatct taagaaattc gaaaaggaaa aaatgattat gaaaaagaaa gaaaaagaaa
      121 gtattgaaac attgtgttat tctaggaata ttaggaactt gtctagctgg cattggtaca
      181 ggaatagacg ttgacgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa
      241 tgttgggtaa attggggaca atcatggtca gaagggttaa aacgttgggg agataatctt
      301 tttggtagtt ttagtggagg acgataatga cttcaataaa cattgatcac acaaaaaaag
      361 tcatacatga attatacaat agtttaatga cacgacctaa tcagacgaaa gcattattag
      421 atattactga tgttttattt caggtatata aaaaaattga tacagcaaaa tatcctgaaa
      481 ttcttgtaaa taaattagtt aattatattt acatcgtggg ttttgataat aaaatacgct
      541 ttattgataa tgatgaaaaa ttattaatag aattagggga tatttctaaa aaagctggaa
      601 ttaatagtaa atacaaagca aactttacag ataaatcgca attttatagt tattcagaaa
      661 caatgcctca acgataaaat atagtata
//
LOCUS       DACDTH010000232          635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDTH010000232 DACDTH010000000
VERSION     DACDTH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.244"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..270)
                     /locus_tag="IWK54_003073"
     CDS             complement(<1..270)
                     /locus_tag="IWK54_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595091.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="IWK54_003074"
     CDS             complement(276..>635)
                     /locus_tag="IWK54_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9595092.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DACDTH010000233          609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDTH010000233 DACDTH010000000
VERSION     DACDTH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.246"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..473)
                     /locus_tag="IWK54_003075"
     CDS             complement(<1..473)
                     /locus_tag="IWK54_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595093.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKE"
BASE COUNT          174 a          105 c          101 g          229 t
ORIGIN      
        1 ttttctttga tgatgacatc gtctacagct tgctgccagt ttgatacatt tttagtaaat
       61 aaatcgttga cagctgtctg gatcttcagt tttgattctg cactatctaa ctgtgtcacg
      121 attgctttct tctcatcttg gattgcaaca gcttctttag gcaattctga ttctttgatg
      181 tcgtaatcat ctgctgaaag tttgacagag gatagcttat ttgacaaact tgtgattgtt
      241 tcttctgtta tttcagggtt tagaaaaatt ttttcttttg tactatacag cttgtctact
      301 tcttttgata aagaagtcaa ggtgtctttt tgattggtga tggcagattc tgcataattg
      361 atccgttgct gatgttgccg ctgattattg atatggtatc caaacaaaca gatcgcaata
      421 gcacctgcac ctaccgccac agcagtcaat gacttgacga atggcttttc catagattca
      481 ctcctaagta ttgatgatag ataaattaag atataattca cttacataaa aatgacagct
      541 ttttatttaa aaaaacataa ttaaactaat atattcacag aaatatttgt aaacaagttt
      601 tccctatat
//
LOCUS       DACDTH010000234          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDTH010000234 DACDTH010000000
VERSION     DACDTH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.247"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IWK54_003076"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IWK54_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9595094.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DACDTH010000235          520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDTH010000235 DACDTH010000000
VERSION     DACDTH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.248"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>520
                     /locus_tag="IWK54_003077"
     CDS             <1..>520
                     /locus_tag="IWK54_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9595095.1"
                     /translation="YPLYSYQKKIGTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQK
                     IDYLDIFPNILIEQLEWLKKKKIKMAIASSSPMDTINEVVTTCKIESYFDCLISGRDL
                     PESKPNPTIFLKASEQLRIPVEECLVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSL
                     ADDVIYDIKYLRQ"
BASE COUNT          200 a           63 c           92 g          165 t
ORIGIN      
        1 ctatcctttg tattcttatc aaaaaaagat tggaactaca gcaagtattt ttgatgatat
       61 agaaaagtac aatgagggat gtaacagttt agaattgaag ggaaaattta ctaaatattg
      121 gaaatcgcaa aagattgatt acttggatat ttttcctaat atactaattg aacagcttga
      181 atggttaaaa aagaaaaaaa taaaaatggc aattgcttct tccagtccaa tggatactat
      241 aaatgaagtt gttaccactt gtaaaataga gtcatatttt gattgtttaa ttagcggaag
      301 agatttgcca gaaagtaaac ctaatcctac tatattcctt aaagcttcag aacagctcag
      361 aattccagtt gaagaatgtc tggtgataga agattcttat aatggtataa aagctggtaa
      421 aagagcaaat atgaaggttc ttgcaattaa agataaacgt ttttctcaag atgtaagtct
      481 agcagatgat gttatttacg atattaaata tctgcgacaa
//
LOCUS       DACDTH010000236          515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDTH010000236 DACDTH010000000
VERSION     DACDTH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.249"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(107..>515)
                     /locus_tag="IWK54_003078"
     CDS             complement(107..>515)
                     /locus_tag="IWK54_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP9595096.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcca
//
LOCUS       DACDTH010000237          513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDTH010000237 DACDTH010000000
VERSION     DACDTH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.250"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(45..>513)
                     /locus_tag="IWK54_003079"
     CDS             complement(45..>513)
                     /locus_tag="IWK54_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9595097.1"
                     /translation="ISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITV
                     NNYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKIS
                     NMDKIFDVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          185 a           74 c           29 g          225 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa ata
//
LOCUS       DACDTH010000238          510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDTH010000238 DACDTH010000000
VERSION     DACDTH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.251"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..510
                     /locus_tag="IWK54_003080"
                     /pseudo
     CDS             1..510
                     /locus_tag="IWK54_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          212 a           88 c           92 g          118 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag
//
LOCUS       DACDTH010000239          508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDTH010000239 DACDTH010000000
VERSION     DACDTH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.252"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DACDTH010000240          498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDTH010000240 DACDTH010000000
VERSION     DACDTH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.254"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IWK54_003081"
     CDS             141..398
                     /locus_tag="IWK54_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595098.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          210 a           65 c           70 g          153 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccat
//
LOCUS       DACDTH010000241          496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDTH010000241 DACDTH010000000
VERSION     DACDTH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.255"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..156
                     /locus_tag="IWK54_003082"
     CDS             <1..156
                     /locus_tag="IWK54_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP9595099.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>496
                     /locus_tag="IWK54_003083"
     CDS             175..>496
                     /locus_tag="IWK54_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9595100.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINRE"
BASE COUNT          221 a           38 c           90 g          147 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaag
//
LOCUS       DACDTH010000242          493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDTH010000242 DACDTH010000000
VERSION     DACDTH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.256"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..365
                     /locus_tag="IWK54_003084"
     CDS             <1..365
                     /locus_tag="IWK54_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9595101.1"
                     /translation="VFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKTM
                     FYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGGK
                     NDYKDEIVYVYDYEKGDK"
BASE COUNT          196 a           35 c           84 g          178 t
ORIGIN      
        1 tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca tttgggttta
       61 tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt ttaagacctg
      121 atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat caagacaaag
      181 gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt ggttgttctg
      241 aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat gaaaaattag
      301 gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa aaaggtgata
      361 aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat gatgattttc
      421 ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat ggtagagata
      481 aaaaatatac att
//
LOCUS       DACDTH010000243          485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDTH010000243 DACDTH010000000
VERSION     DACDTH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.257"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(62..259)
                     /locus_tag="IWK54_003085"
     CDS             complement(62..259)
                     /locus_tag="IWK54_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9595102.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           67 g          171 t
ORIGIN      
        1 aatttttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DACDTH010000244          454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDTH010000244 DACDTH010000000
VERSION     DACDTH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.259"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..302
                     /locus_tag="IWK54_003086"
     CDS             <1..302
                     /locus_tag="IWK54_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9595103.1"
                     /translation="FMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          174 a           67 c           63 g          150 t
ORIGIN      
        1 ggtttatgat agataaatgg aaaaagcagt atccacgagt ggttaaattg ctcatgaatc
       61 ctgctatatt aaccttttat aatttccctc cgtcaatcag aagaactatt tattcaacta
      121 atttgattga aggttttaac aaacaattaa agaaatatac caaaagaaaa gagcaatttc
      181 ctaatgaaga atctctggag agatttctag tatctcaatt caacaactat aatcaaaagt
      241 ttctatgccg agttcataaa ggttttaaag aaatacatga tacattagaa tcaatgtttt
      301 aacttgatca acagaatggg cttttccatt tacacaaaat tattgacgct cccgacaaaa
      361 ttatgatctt gcgtaaagtt ataaaatatt aataaaaata ttttattaat gaaaagcttg
      421 gtagcacggt tattcatatg ctaatttatt tcac
//
LOCUS       DACDTH010000245          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDTH010000245 DACDTH010000000
VERSION     DACDTH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.260"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IWK54_003087"
     CDS             complement(<1..339)
                     /locus_tag="IWK54_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9595104.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACDTH010000246          386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDTH010000246 DACDTH010000000
VERSION     DACDTH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.261"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            22..>386
                     /locus_tag="IWK54_003088"
     CDS             22..>386
                     /locus_tag="IWK54_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595105.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKL"
BASE COUNT          160 a           44 c           70 g          112 t
ORIGIN      
        1 tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat ttccaaacaa
       61 attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc tgtttcaaga
      121 gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta tgatattatt
      181 tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt ttcaacagtt
      241 aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga gaaaagaaaa
      301 tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct gatgaatcga
      361 gaagcagaaa aaataataaa caaact
//
LOCUS       DACDTH010000247          375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDTH010000247 DACDTH010000000
VERSION     DACDTH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.262"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWK54_003089"
     CDS             complement(<1..132)
                     /locus_tag="IWK54_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9595106.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DACDTH010000248          371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDTH010000248 DACDTH010000000
VERSION     DACDTH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.denovo.263"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            18..371
                     /locus_tag="IWK54_003090"
                     /pseudo
     CDS             18..371
                     /locus_tag="IWK54_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          152 a           36 c           83 g          100 t
ORIGIN      
        1 attaggagga gatgtagatg aaaaagttta aaaagaaaat atatttagga ttggttgttg
       61 gggtgagtat gtttgggttc atggctcata ctaaacaagt aactgcagat actaaccaaa
      121 aagaaaggat tatgagccaa ttagatgact ctcaaataag tatgtaccca atgagctaat
      181 tgtcagttta aaggctacac aaagaatttc aacagagaaa aataaaccgc aaaatttatt
      241 gggtgtaaaa gttatggtgg aaaaagattt gactcttagg ataaatgaaa gcgattataa
      301 taagcgagat caagtattat tagtgaaatt gaacgaagaa attagtgtgg aagaggcagt
      361 aagaacattt a
//
LOCUS       DACDTH010000249        13536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.guided.074, whole genome shotgun sequence.
ACCESSION   DACDTH010000249 DACDTH010000000
VERSION     DACDTH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.guided.074"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            292..567
                     /locus_tag="IWK54_003091"
     CDS             292..567
                     /locus_tag="IWK54_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595107.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            580..2652
                     /locus_tag="IWK54_003092"
     CDS             580..2652
                     /locus_tag="IWK54_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9595108.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2817..4703
                     /locus_tag="IWK54_003093"
     CDS             2817..4703
                     /locus_tag="IWK54_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9595109.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4715..5662
                     /locus_tag="IWK54_003094"
     CDS             4715..5662
                     /locus_tag="IWK54_003094"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9595110.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5682..6209
                     /locus_tag="IWK54_003095"
     CDS             5682..6209
                     /locus_tag="IWK54_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9595111.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6272..6925)
                     /locus_tag="IWK54_003096"
     CDS             complement(6272..6925)
                     /locus_tag="IWK54_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9595112.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7057..7899
                     /locus_tag="IWK54_003097"
     CDS             7057..7899
                     /locus_tag="IWK54_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9595113.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8057..8953
                     /locus_tag="IWK54_003098"
     CDS             8057..8953
                     /locus_tag="IWK54_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9595114.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8956..9669
                     /locus_tag="IWK54_003099"
     CDS             8956..9669
                     /locus_tag="IWK54_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9595115.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9689..10207
                     /gene="msrA"
                     /locus_tag="IWK54_003100"
     CDS             9689..10207
                     /gene="msrA"
                     /locus_tag="IWK54_003100"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9595116.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10204..10431
                     /locus_tag="IWK54_003101"
     CDS             10204..10431
                     /locus_tag="IWK54_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9595117.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10583..11134
                     /gene="lepB"
                     /locus_tag="IWK54_003102"
     CDS             10583..11134
                     /gene="lepB"
                     /locus_tag="IWK54_003102"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9595118.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11217..11759)
                     /locus_tag="IWK54_003103"
     CDS             complement(11217..11759)
                     /locus_tag="IWK54_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9595119.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11996..12394)
                     /locus_tag="IWK54_003104"
     CDS             complement(11996..12394)
                     /locus_tag="IWK54_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9595120.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12654..13514
                     /gene="ylqF"
                     /locus_tag="IWK54_003105"
     CDS             12654..13514
                     /gene="ylqF"
                     /locus_tag="IWK54_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9595121.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         4718 a         2306 c         2739 g         3773 t
ORIGIN      
        1 ttctgattca tcagtcccat aaattataga gccgattttt gtcgcagccc catttttttg
       61 ttgtgataaa agaacaagaa aggcaggaat gggaaagtaa acatcctttt gtcagaaact
      121 tttatctttc ttaagtttta cttgatatct gatactattt ggaatagaat gaggcgttga
      181 aaaatttatg gaaatttcta actagcaata aacgatgaaa caatgaaata tttttcatcc
      241 aaaaagaact ttgctataat gatggaagtt tgtgtatagg agatctggaa aatgagagag
      301 agttttgaac aacaaaaaaa actgttgcat gatcgctatg gcgccctttc gatggatgac
      361 cgccgtcaaa tactttgtaa gttgcgtaaa agaaatatat tgatgtatcg tcagttggaa
      421 cgtctgaaac atgatctgct tcgtttggag tcaaaacgtg tacagtgtga attagagggg
      481 aaccaaacac aggttgaagt agtcgaaacc aaaattttaa agaaaaaaga acaattttta
      541 aaaatgctta cacaaaataa aaaataaagg ggatggacga tggaatttgc tgagttagtt
      601 attgtatttg cagtaacgat caccttatcg aacatagcta gccgcatttt gccaatgatt
      661 ccagctccac tgatccaaat tttccttgga gtggtattag gtctgacaga gtggggacaa
      721 tcaatcgatt ttgaacctga attgttctta gttatgatta ttgcaccatt gttatttaga
      781 gaaggagaaa aagcagatat ttcttcgatt ctcaaaaatt tcgggacaat tttgttcttg
      841 gcatttggag gagtcatttt gacccttgtc ggggtaggtg cgacgttatc attcctgttg
      901 ccaagtgtcc cacttgctgc ctgctttgct tttggagcag cgttaggtcc gacagatgcg
      961 gtagcagtca gttctctttc tggacgagtg aatatcccta aaaaggcgat gcatattttg
     1021 gaaggagaag gtcttttgaa tgatgcatct ggtgttactg ccttccagtt tgctcttggt
     1081 gcgctgatca ccggatcttt ttcagcagta aacgcgggga tgagtctagt agtttctagt
     1141 attgggggag ctttgatcgg ctttttgctt gtctggttca aacaaaaaat aattcatttg
     1201 atcgaaaaag catcagcaca agatgtcaca gggtatctgc tgatcgaact tttgcttcct
     1261 tttctagcct atgtattggc agaggcattt gatgtatctg gaatcattgc tgcagtggca
     1321 gcaggtattc tccaagcttc tggttttagg aaaatctctg tgtttgatgc agaattgtcg
     1381 agcttgtccc acagtacatg gacaactatt gcttttacac tgaatgcatt agtatttata
     1441 tttttaggaa tcgaattgac gcaagtgttc tcgccagtat ggggagacgg tttatatcca
     1501 aacggtcttt tattagcgat tatcgtatta atctctgtca tgctttttgt gatcagattc
     1561 atttctatca gtctttttta tgtattcaaa gatggcagta aaaagttcaa aaaacagttg
     1621 aacgagatct tgatcttaac atttggcggt gtaaagggaa cagtcagttt ggctacgatt
     1681 tttattttgc cattttcaat caataatatg atgttttacc agcggtcttt gcttttgttt
     1741 ctgacggcgg gtgtcatctt agtgactttg gtcataggga tcattgtctt gcctatgctg
     1801 acggaaacag aggaagcgaa aagcacggat ttgaatgctt tgatgatttt agaagaggtc
     1861 gttgaagttt tgcgaaaaga aataaaggaa attgatcgta atacaaaaga atttttagcc
     1921 actgaagcag tgatagaaaa ttatcaagaa cggatacgtg atctgtactt ggaagattta
     1981 accgatgatg aaaagcaaga agtacaagaa atacaagcac tgattttgtc gatcgaacgg
     2041 gatggattgg atgaaagtta tcgttcgggc aagctttctt ctaatggtta tcgtttttac
     2101 tcccgttttc tttctcgatt cgaacattct atcacaagcc agattttgtc ttttatcggc
     2161 ttttggttca ttgttgtccg taggttaatg cgaatcgttc tccatccgaa aatgttttgg
     2221 caaagaagat atgctgatcg tcagacattt attagtgatg aagatatcca agaaatcaga
     2281 gagacatatc aaaaaaatac tcagttgatt attgaaagtt tagataactt gactgatatt
     2341 tatgatgaaa cgatggttca attttttatc cagcaacgca aacttgaagg aataaaaatg
     2401 aactcgggga atttgatttc ttcgtggatg atccagcaag atagtctgtt tacgaaaaaa
     2461 atgctaagag gttattatct tgaaagaaaa atcattgatg aatacgaagt tgctgaaaaa
     2521 atcacgacgt tttctgcgaa cagctatcgc aggaatatca atttgttaga atcttatacg
     2581 atgaacaaac catctgataa cttttctttc cgatttgcgt ttcgagcaaa gtcaaaaaaa
     2641 cgagtccaat aaaataaaaa ggtaaaaatt ttcaagtttt ataaaaaaca aaacatatca
     2701 gaacaggatc gaatcggtcc tgttcttttt ttgagaacac aaaaagaaca aataccaaaa
     2761 aaattttcct gaagttcact atttttatga tacaatatcc aagataagga gggaacatgt
     2821 tgaaagaatt aaaagtaact gacctcaaaa aaacttatgg tgaaaaagat ttgtttgacc
     2881 agatttcttt tttggtccac gaaaaagacc gtatcgggtt gattgggacg aacgggacgg
     2941 gtaaaacaag tctcttaaat attattgccg gtatcgatag cagcgacggt gatcggcaaa
     3001 cagtgtttta tccaactgat taccgtattg gttatctttc tcaagcagct gaattttccg
     3061 atgaattaac tgttctccaa gcagtctttc aaggaaacag ccccttgatc caaactgttc
     3121 gagcatatga ggaagcattg atcgaactag gggaaaacgg agaagaccct gatgtgcaaa
     3181 aacgttatgc taaagcagaa gaacaaatga ataaagaaga tgcttggact acagatacca
     3241 acgcaaagat tattttgcaa aaattaggaa tcagtgaatt agataaaaaa atcagtactt
     3301 tatcaggtgg tcagaaaaaa cgagttagtc tggcgcaagt gttgattgat gaacccgatt
     3361 tactattact agacgaacca acaaaccatt tagattatga agcgatcgag tggttggaaa
     3421 actatttgaa acaataccgt ggcgcactat tgatggttac ccacgatcgt tattttctag
     3481 atagagtagc aaaccgaatc ttcgaacttt catttggaaa attatacgag tataaaggaa
     3541 attatgaagc ttacgtctta gaaaaagcag aacgtgatcg tgtagaagta gaacaagaag
     3601 aaaagcgcaa gcggctttat aaacaggaat tagcttggat gagggctggc gtacaggcac
     3661 gtggcaccaa gcagcaagca cggatcaacc gtttcgaaga tctaaaggag aacttgtacc
     3721 aagtaaacca agaagatgat ttagagctga acctagcgac acaacgttta gggaaaaaag
     3781 tactggaaat caaagatggc agttaccgta taaatgacca aacactgcta gaacatttag
     3841 atttattgat tcaatctcgg gaaagactag gtattactgg taaaaatggt gcgggaaaat
     3901 cgacattgct taatattcta gctggcagaa ttcccctgga tagcggtaca atgtctatcg
     3961 gtgaaacggt tcatcttgcg tattatacac aagaaaatga agaaatggca ccagataaac
     4021 ggatgattgc ttacttacaa gaagctgctg aagaagcaaa aacagcagat ggcagtcaaa
     4081 tcggagtagc agaattactt gaacgatttt tatttccaag atttatgcat gggacactga
     4141 taggcaaact ttcaggcgga gaaaaacgcc ggttattttt gttgaaactg ctgattcagc
     4201 aacctaatgt tttattattg gatgaaccga ccaatgattt ggacatagca acattgacga
     4261 ttttggaaga ttattttaga agttttccag gtgctgtgat cactgtttcc catgaccgtt
     4321 atttcttgga caaggtagca gataagttat tagtattcca aggaaacgga aaacaagaac
     4381 tttattacgg aaacatgtcc agttatttac tgaagcaaaa agagacccag cagcctgctg
     4441 aaaaagcgaa accaaaaata cagagcaagg aacctgctga aaagaagaaa ttatcttaca
     4501 tggaacaaaa agaatgggaa acgattgaag atgagattgc agaacttgaa gaaaaaatct
     4561 cattgctcca agaagagatg aatcatcaag gagataattt cactcgatta caagaattgc
     4621 aaaacgacgt atctgaaaca gaagcacaac ttgaagaaaa aatggcacgg tgggaatact
     4681 tgagtgaatg ggtagaagat taggaggaaa aaggatggaa caagcgtatc ttgacttagg
     4741 aagaaaattg ttggaagaag ggcatcgtaa agaagacaga acaggcacag gcacattaag
     4801 cttattcgga taccaaatgc ggtttgatct gcaaaaaggg tttcctttgt taacaacgaa
     4861 acgagtacct tttggattga tcaaaagcga attgctttgg tttttaaaag gagatacgaa
     4921 tatccgctat ctattacaaa ataaaaacca tatttgggat gaatgggcat ttgaacgcta
     4981 catcaaaagt ccagactata atggaccaga tatgactgat tttggccata gatgtttgac
     5041 agatcctgaa ttcaatgaag aatataaaaa gcagagcaag cttttttgcg agaagatcct
     5101 aaccgatgat tcctttgctg aaacatacgg cgatcttgga cacatctacg gctatcaatg
     5161 gcgtcactgg gaaacaaaag acggtggctt tatcgatcaa atcaaacaag tgatcgaagc
     5221 aatcaaaaag actcctgatt ctagaagatt gatcgtttct gcatggaatc cggaagatgt
     5281 tcctagcatg gctttgcctc cttgtcatac gatgtttcaa ttttatgttc aagagggacg
     5341 gctaagctgc caattgtatc aacgcagtgg ggatgtgttt ctaggcgttc cgttcaacat
     5401 tgcaagttat gcgttgctga ctcacctaat tgcacatgaa acaggtttag cggttggcga
     5461 atttgttcac acattaggag atgctcatct ttatatcaat catatcgaac aaatgaaaga
     5521 acaattgagc cgagaaatac gcgcatttcc gactttagtt ttgaatcagg aaaagcagtc
     5581 agtatttgat tttgagatgg aagatatcca attagaagga tatgacccac atccggcgat
     5641 caaagctcct atcgctgttt agaatcagaa aggatgagaa aatgttagct gcaatttggg
     5701 cacaggatga aaacggattg attggaaaag aagaccgtct tccttggcgt ttgccaaatg
     5761 atctgaaatt cttcaaacaa atgacagaag caaatacact agtgatggga cgaaaaacat
     5821 tcgaagggat gggtagccgt ccgttgccga atcgtcagac gattgttttg acgagagatt
     5881 cttcctataa agcagaaggc gtgcatgtca tgcatagtgt ggaagacgtg cttgctctgg
     5941 aaaaagaaac agacggtatc tttttcatcg ctggagggtc agctgtttat caagaatttc
     6001 tgccattttg tacgatatta taccgtacag tcatccatca tgtattcgat ggagatgctt
     6061 attttccacc tgttgactgg tctgattggt cactgatcaa tactagtcaa ggagaaatgg
     6121 atgaaaaaaa tcgttatccg catcaatttg aaacgtatca gcgtaatgag aatcctgtaa
     6181 aaagcgggac aggcgaattg acgatataaa aaagctatgg atagactagc tgttatttag
     6241 agctgtccca tccatagctt tatttttttt attaagtaaa gaataaaaca gagaagtaca
     6301 taaaacctgc acctaacata acaaacaaat gccaaacaac atgcatatag cggatagaat
     6361 tcaagctata aaagaatgcg cctatcgtgt aagaaagccc gcctgctacc atcaaaataa
     6421 caccagtcat tccaagagag tgataaagag gaacagcagc cacgacacag agccacccca
     6481 taaaaatata gatgatcgta gatacttttg atactctctc ccgtttatgc aacgtcaacg
     6541 ctttatatat aatgccaaga atcgcaaaca accagattaa tacaaataaa gtccaaccta
     6601 gccatcctcg gatactcagc aaacaaaaag gagtataact tcctgcaatc aaaagaaaaa
     6661 tcgaactatg atcgaatact tggaacacct tttttgcttt tgtaaaaatc aatgaatgaa
     6721 acaaagtaga cgttaaaaat aaaagaatca tcatcgatcc atagatagca taagagacaa
     6781 tatggaggga ggaatgcaag tgggcacctt ttactaaaag aaccactagt cccgcgatac
     6841 tcaatccagt cccaattcca tgggtgactg cattaaacac ttcatttaat atttcatacg
     6901 ttcgattaaa tttcgttttt tccatgaaaa cggctccttt actaggtgta attaatgaaa
     6961 aatcattatt tgtctgttat actaaaaata gaaattgtct tacatgtagt ttaacatgaa
     7021 taaggcatct tgttaaagaa agagagagag aaaataatga caaatgtaaa aatcgtaaca
     7081 gattcttcat gtacgatgga aaaaaatgta cgcgatgctt taggcattcg tgtcatgccg
     7141 ctatccatca tgatcgacgg aacagtatat cccgatgatg accaactaga cggcgaaaga
     7201 tttatggaaa tgatggcgaa agcaaaaaat ctgcccaaaa caagccaacc accaattgga
     7261 gaattcatct cgatgtatga tgagctagga gaagacggca gtgaagtgat ttcgatccat
     7321 atgacaaaag gcttgagtgg aacagtagaa gcagcaagac aagccagtaa tctaacaaaa
     7381 acgaaagtaa cggttatcga cagtgatacc actgaccaag gattatcttt tcaggtaatt
     7441 cgagcagctg aattagcaaa agaaggtaaa acagtagaga aaatccttga agaagtcaaa
     7501 aaagtacaag acaatacaaa attatacatc ggaatatcca cactagataa cttggtaaaa
     7561 ggtggaagaa tcagccgaac aactggtttg ctgtctagtg ttttgaacat acgcgtggtg
     7621 atgaatttcg cccattctga attaatccct gtaactaagg ggcgaggaaa gaaaacgttc
     7681 gataaatggt ttaataattt gaaaaatgaa ctgaaagaat tgccaaatgt gcgacaaatc
     7741 ggtatttcac atgcggatgc aaaagaattg gcgcaagaat tcaaagaggg tttgcaagaa
     7801 ttattcccgg acatgcatat ccctgtactg catacgaatc cggttatcgc tacgcatact
     7861 ggtaaagacg cgtttgcaat catgtattat gtagattagt cgaaaagagg ataagccctc
     7921 agcctgaaag gcttgcagct tattctcttt ttctttataa aattaggaaa ataattgttt
     7981 tcttttttca taaagactac ctttttgata aaatttttga taacattaga accaatcgaa
     8041 tacaagaggt gaaaaaatga aattaacgaa acgacatttg atcctcgttt tgttcccatt
     8101 attaattgcg atcatcatct atgcaggttt agctgcttct ataccgaaag ctggcccact
     8161 aatgaaacca gtaactactg tgagagaaga tccaaataaa aaagaaaaac ttcactacat
     8221 agctatcgga gattcgctga ctgaaggagt aggagatcaa accaagcaag gaggattcgt
     8281 tccccttgtc gcaaatgatc taaaagaccg ttataatttg accgcggttg aaacagaaaa
     8341 ctatggtgta aatggtgaac gaagtgacca gatcttaaag cgagtaaaaa caaaagaaga
     8401 aattcgaaac aatatcaaaa cagctgattt tatcacactg actgttggcg gtaatgattt
     8461 gatgaaagtt atccaaaata atttattcgg gttaacggtc aattccttta aaaagccact
     8521 gaaaaactat caggaaaatg tcacagaact attggaagaa tttcgatccc tcaataaaac
     8581 cgcacctatt tacattttag gcatctacaa tccattttat ttgaactttc ctgagatcac
     8641 tgacatgcag acaattgtta acaattggaa caaaggaaca gaaacggtga caaaaaaaat
     8701 gccgaactgc tactttatcc caatcaatga tttgctttat caaggattga atcaggaagt
     8761 aggtgtcaca gaagaaggaa cagatcaaac aactagcacg acagaaaatc taaatggcat
     8821 caaaaataat gtgctctatg aagaagatca tttccatcca aataatttag gttatcaatt
     8881 gatggcaaat gcagtgagag atcagctgat cgagacacaa aaccaatggt tagagaagga
     8941 ggatgatcaa taaacatgaa aacaagatct gacgtatctt caaacaaaac aaagaaaaca
     9001 aattactgga aattcgcctt ccttatcctt cttggggtag tacttggttc gaccgtattt
     9061 ttaggcagcc gtatttttgc taatcgggaa ccagaactcc cagaaatacc agctcttacg
     9121 gaaagacaag gagatcctgt cttaacgatc aattcaaata aagaaaaagt caatcaaatc
     9181 atcagctttt tccttagtga gtatcaaaaa gatagtgata ttgaatataa attttatttg
     9241 gaaaatgaag cactactcaa tggtactttc gaagtactag gttttcctat cgatttctat
     9301 ttatattttg atccgtatgt gatggaaaac ggaaatgtcc aactaaaagc tagaagtctt
     9361 tctatcggaa cattgagttt gccaatcaaa gatgtgatga atatgatcaa gagaaactat
     9421 aaattaccag attggatcga agtcaataca gatgatctga ctatcatgct taggttggat
     9481 caattccgaa tgcaaaacgg gatgtatatt aaagcagata aaatcgactt agtaaatgat
     9541 gacattcggt ttagtctata tttgccaaaa gatacctctg atgcttcaaa caactcaaat
     9601 accaacaaaa ctagcaccag cagtactgaa agcaagacaa aagaaaataa aaaatcatcg
     9661 gatgagtagt attagagggg gaatcataat ggaacgagca atatttgcag gaggctgctt
     9721 ctggtgtatg atccagcctt tcgacacgct tcctggtatc catacagtga tgtcaggata
     9781 tactggaggg catgtaccag atccgactta tgaacaagta aaaagccaaa cgactggaca
     9841 tacagaagca gtggagatta tctatgatcc aacattgatt tcatatgaac aattagtaga
     9901 actttactgg cagcaaactg atccaactga cgctttcgga caattcgagg atcgcggtga
     9961 caattatcgc ccggtgatct tttatttgac agaagaacag cggatcattg ctgaaaaaag
    10021 taagaaacag ttagctgaaa gtggacgttt cgaacagcca atcgttacaa aaatcgaacc
    10081 agcccaacca ttttatctag cagaagaaga ccatcaagag tattataaaa agcatcctga
    10141 aaattttaaa agaaaccatg cacgccgggc tgcctttatc gaagaaaatt ggaggaacga
    10201 agggtgaatc gaagcttttt tcattattta atgacactgc gaggcggtgg cacacatgat
    10261 gctttatctg ttttcgctac agaagccggt aaagacatcc aatttcctaa gcacagctct
    10321 agctacgaag aaatttctaa ttatctggaa ctgaatgtcg actatttgcc gtccatggat
    10381 atctttgacg aagcatggga aaaatattta gaaaataatc atgacatata agagatagtt
    10441 ttgtacttta tgatcaaaaa agttttttgt ccaatagcct aattgaaaga attattttta
    10501 tttcagctat ctagtcgcat ttttttgata tttttcttat aataaagaaa gaatgaaaaa
    10561 aagagggatg gattgataga gattgacaaa aaaacaacgc tatattaatc gcttctggct
    10621 tgtattcaaa tacttacttg tttctgtatt ccttgcgttc atgttgcggg gatttttatt
    10681 catcccggta ccagtggaag gcaattcaat ggaaaacgtg ttgaagcaag gtgatatggt
    10741 tgtgatggaa aaattttcgg agatcagacg atttgatatt gttgtttttc aattagcaga
    10801 tggcacgatt tatatcaaac gagtaatcgg tcttccagga gaaaatgtca gctaccaaaa
    10861 tgatcaattg aagatcaatg ggaaagtagt taaggaacct tatctaacta aaaacctgaa
    10921 atcagatcat gcaaatgcat cctatacgac ggactttaca ctacaagagc taacgggaca
    10981 atccaagctt ccggaagata attattttgt acttggagat aatcgaagag tttcaaaaga
    11041 tagtcgttcg tttggcacga tcaataaaac tgatattcta ggtaaagcaa gatttgtcta
    11101 ttatccactt gatgaaataa aatggatcca gtgagaaaca ataaagcgtg cagtctgttg
    11161 agactgcacg ctttattgtt taatccaaaa tcagtttact accttagtac gtaaaatcaa
    11221 acagctcgtt taaacacttt tctagcctct aaagttttga taaatgaata aacaaccatt
    11281 atttgaatag ggatttggat catattcctg atgaatctac ctagaaaaat tactttgaaa
    11341 tctgcaccaa acaaaacaga catcagccaa gaattgataa aaaggctgac tgggatcata
    11401 atcaataatt gggcagcgat gacggaaggc aggtttatct ttttatgata taaaaataag
    11461 ccattaataa atccaccaag gaaagccgtg ataagaaaag cagcttgaaa cgttcctgaa
    11521 ccaaatatcg tgaaatttaa agtgttcgac aacatttcaa gcaaaccagc ccagatcggt
    11581 ccatatagat aacccataag cgccatactt aaaaatccga atgtgatttg aaaataagca
    11641 ggaccaatcg ctatcttgta taagatgaca tcaagggcta ataaaagagc agaggtcgac
    11701 aacaatcttg cattcatttc gggcaatcta aaaacgtctt ttgcaatcaa agtgttcaaa
    11761 atattctctc ctttgtcagt taataataaa aggataccat aaaatcacca atgtgtcatg
    11821 acacattggt gatttacata cactggcatc taacaattaa aaaaagggtg agaagactgc
    11881 ttttataaac tgtaaaaaag agattgtacg aataaaaaaa atagaggcgg agaatactta
    11941 tcgtatcatc cgcctttgtt tttttgttat aaaaaacgtc agattagtta tctgcttaca
    12001 tcttcatcca tacgggttct aatgtatttc cgtctagatc ttggacttct aatgaaaaca
    12061 tttcttcttc aggcattccc atatctactt cgtggaaagt accaccattt tcttttgcaa
    12121 tttgagcaaa tttttttact tcatccgcgt tttcaacact aaacgaaatc aaagcaccac
    12181 ttgttttttt attatcagct atttctttat cttttataaa ttttttataa aaatcatgag
    12241 agagaagcat tatccaaaaa gtgtcatccc ataccattga accagcttct tcagtagaaa
    12301 attcttcatt ttttttaaaa cccagctttt cataaaaagc aacggaacga ttgacatcag
    12361 caacaggaaa attaacaaaa accatactat tcatttgttt cgtctccttt ctgttttgtt
    12421 cgtctatcat ctcttattta ggaaacgctg tcaaaaattt agattcatat atagaaacca
    12481 aaataaaaat ttgcatcatc aaaaaagtga agatgtgata gaatgaggac tggttcactt
    12541 tcacttaact aatagatgga atttctttat aataaaaaag tgaatttata ttgaaaaaga
    12601 aaaaaacaag taaagccggg gaatattccg gtatgaaagg aatggagaga acaatgacca
    12661 tccaatggtt tccgggacat atggcaaaag cccgacgaga agtttctgaa aaaatgaaat
    12721 atgtggacat cgtatttgaa ttggtagacg cacgattacc tttatcttct cgtaatccaa
    12781 tgctagatca gatcattcag caaaaacctc gccttgtttt attgaataaa gcggatctgg
    12841 cagatccaca acagacaaag ctgtggcaag aatattttcg taaacaaggc catcttgcat
    12901 tagctatcac ggcacaagaa agtaaaggaa ttaaaggtct agtacctgcg gctaaagaag
    12961 cattgaaaga aaaacgggca agagatgctg ctcgggggat caagcctcgc gctattcggg
    13021 caatggtctt aggtattcca aatgttggga aatctacgtt gatgaatcga ctagtaggca
    13081 agaaaattgc acagacagga aataagcctg gcgtaacaaa aggtcagcag tggcttcgtt
    13141 taggtaatga gttggaattg ttagacacac cagggatttt atggcccaaa tttgaagatg
    13201 aagaaatcgg caaaaaacta gcgctgacag gcgcgatcaa agaccagctt gtccatttag
    13261 atgatttagc gatttacgga ttagactttt ttgcccgata ttattcagga agaatcagcg
    13321 atcgttatgg tttatcaatc gagcaagaag aaaaattggc accggaatta ctgatggata
    13381 ttacagcaaa acgtgggtat aaagaagact atgatcgtgg cagcgaatta gtcgtgcatg
    13441 atattcgtca aggaaaactt ggacgataca cactggatcg ttgcgaagaa atggagcaaa
    13501 aaagtgatga ctgaatccat ccaaagtatc aaagaa
//
LOCUS       DACDTH010000250        10104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.guided.088, whole genome shotgun sequence.
ACCESSION   DACDTH010000250 DACDTH010000000
VERSION     DACDTH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.guided.088"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..324
                     /locus_tag="IWK54_003106"
     CDS             <1..324
                     /locus_tag="IWK54_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9595122.1"
                     /translation="RAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTNIENKGQHPST
                     QVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLMEALAKGINEA
                     RNIEG"
     gene            complement(382..570)
                     /locus_tag="IWK54_003107"
     CDS             complement(382..570)
                     /locus_tag="IWK54_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9595123.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(668..1708)
                     /locus_tag="IWK54_003108"
     CDS             complement(668..1708)
                     /locus_tag="IWK54_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9595124.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            2070..2567
                     /gene="dfrG"
                     /locus_tag="IWK54_003109"
     CDS             2070..2567
                     /gene="dfrG"
                     /locus_tag="IWK54_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9595125.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(2639..4591)
                     /locus_tag="IWK54_003110"
     CDS             complement(2639..4591)
                     /locus_tag="IWK54_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9595126.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4598..4894)
                     /locus_tag="IWK54_003111"
     CDS             complement(4598..4894)
                     /locus_tag="IWK54_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595127.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(5069..5872)
                     /locus_tag="IWK54_003112"
     CDS             complement(5069..5872)
                     /locus_tag="IWK54_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9595128.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            complement(5888..6004)
                     /locus_tag="IWK54_003113"
     CDS             complement(5888..6004)
                     /locus_tag="IWK54_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9595129.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(6249..7178)
                     /locus_tag="IWK54_003114"
     CDS             complement(6249..7178)
                     /locus_tag="IWK54_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9595130.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            complement(7195..8217)
                     /locus_tag="IWK54_003115"
     CDS             complement(7195..8217)
                     /locus_tag="IWK54_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9595131.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            complement(8214..>10104)
                     /locus_tag="IWK54_003116"
     CDS             complement(8214..>10104)
                     /locus_tag="IWK54_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9595132.1"
                     /translation="NEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAIT
                     NFIWTISLYLSNATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGF
                     LLLFILIVGIYVAYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSK
                     DVSTASLNIGTKIVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEE
                     RVESLLSTSPDTNNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISI
                     FVFLLTGIMIFSQVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITL
                     IITVAFSISTMLYSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGR
                     QVFRRPRQMMNRQTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTP
                     KKETALSNVKKQQGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQ
                     GKENVTHNVTDFKQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPS
                     SPVQNNQQHVRKRQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDK
                     RGQQT"
BASE COUNT         3040 a         1914 c         1698 g         3452 t
ORIGIN      
        1 agagccgttg gtttagcgat taaagaggct cgaatgaaac ggggtctcac tcgtgaacaa
       61 gtgggaacaa tgattgaaat tgacccacgt tatttaacaa atatagaaaa caaaggacaa
      121 cacccaagta cacaagtgct gtatgatctt gtatcattac tccatgtatc tattgatgaa
      181 tttttcttac ctacagataa tttgattaaa agcactcgaa ggttacaggt agaaaaatac
      241 atggatagct ttacagacaa agaactatcc ttaatggaag cactagcaaa aggtatcaat
      301 gaagcaagaa atattgaagg ctagtcagta aaattcagat aaacaaaaag agccgataag
      361 atgagagttt catttctcaa attatcggct ctgcgtcttg gcgtctggct ctttgattgt
      421 tattatattc attttttgaa cattaattaa agtttcgttc ttacatttgg ggcagtataa
      481 agggaaattt ttaagtatag tatccactcg tattcgtagt cttgttttat ttccacaaat
      541 aggacacaat atccacttgt agtttataat aacaatctcc tcctttccac tttaattcaa
      601 atctatatta aagaatattt catcttattt aataagaaac catatttata taacaacata
      661 aaacgcacta agttatttta ttgaacatat atcttacttt atctatccga ctatttagac
      721 gacgggtctg gcaaacaggt tcgccagtgg taacctgata tccttttagc tctgctaaac
      781 aaacactaag cccatttgta aaaaaagtta aatcattgcg ataatcttga atacatcgag
      841 caggaatttc tccaataata atgacctcat tatttttcag ttgagtattt acgatatttg
      901 cacaatattt gggagcatcg ttatatgccc gtgaaagata ttcctgtggt gcataaactt
      961 taaaactaag atatggctct aacaattctg ttccagcttt tctaaaggct tgctccagta
     1021 caataggagt aagcatccga aaatctgctg gagtactaac agggctatag tataaaccgt
     1081 acttaaaaca gattttacaa tccgtcacat tccaaccata taatccttgt tcgcaaccat
     1141 agcgtatccc ttccataact gcattttgaa atgattgatt taagtatcca agagaaaccg
     1201 agctctcata ctgcattcca cttcccaacg gaagcggtga tacagataaa ccaatggaag
     1261 cccagaaagg atttggcggc acttcgatgt gaatggtata ttctgcattt tttaacggtc
     1321 tctccatata aatgactgta ggctctttta gttctatctc cacatgatac ttttcttgca
     1381 acagtgcact aatcacttcc atttgtactt tccctaagaa agaaagtata atttcatgtg
     1441 tcgtagaatc cacgtaatat cgtagaagcg gatcactatc tgagatttcc aaaagggcat
     1501 caagcaacat ttctctctgt tcaggtttac tcggttcaac agttgtttgt agtagagggt
     1561 gcggattttc aatctttttt ctctgtggca atagttttgt atctccaaga acactattta
     1621 acttcaaaaa ctcattttgc aaaataacaa tttctccaga ataagctcta tcaatcttac
     1681 ataattcacc atttattgaa gtatacattt ctgtaacttt tattttttct ttttctgata
     1741 ctctaaccga atctcgtaaa tgtagtactc cactataaag gcgtatatat gcaagtaagt
     1801 agttcagttt tggagtacaa aatgacaaaa tgtacttcaa aatggactac ttttttcatt
     1861 ttaacttaca taccaaatta catacaaaat gatttataaa atgcaattga aaatgataat
     1921 tgaatgaaaa tttgtgattt tgtggtatta tgataatata acttatagaa aaaattgctt
     1981 attcaactaa cgggcaggat aatgagaaaa ggttttttga tagaaataag aaattagatg
     2041 gtaaattagt taaaaaggct gtgttataaa tgaaagtttc tttgattgct gcgatggata
     2101 agaatagagt gattggcaaa gagaatgaca ttccttggag gattcccaag gactgggaat
     2161 atgttaaaaa tactacaaag ggacatccga taatattagg taggaagaac cttgaatcaa
     2221 tcggaagagc cttacctgac agaagaaata ttattctgac gagagataag gggtttacct
     2281 ttaatggttg tgaaattgtt cattcaatag aagatgtttt tgagttatgt aaaaacgaag
     2341 aagaaatttt tattttcgga ggagaacaga tttataattt gtttttccct tatgttgaga
     2401 aaatgtacat cacaaaaata catcatgaat tcgaaggaga tacttttttt ccagaagtga
     2461 attatgagga atggaatgag gtatttgccc aaaaagggat aaagaatgat aaaaatccgt
     2521 ataactacta ttttcatgta tatgaaagaa aaaacttatt gagttaacgg gtgcgttaat
     2581 tgaagaagaa cagtaaagat aaagcacaag agcttgggat tactccaagc tctttttgtc
     2641 atttgaaata aattatatct ttccaatcaa attgtttatc tgtaatccct aatctttgag
     2701 caggtgtcaa aaccttttta tccgctgatt taaaagggcg acaaaagttg tagtaagttc
     2761 ttagaattgt aatcgcaaat tgagcatact tcggattgaa gttagcgtaa atatagctct
     2821 tcttatcacc acgagctgtt gttaatggtc tttcaagaga tgatatataa cgtcttatct
     2881 gttgaataaa gctattggtc gaatggtcat taacatttaa tagcatcttt gcgatttcgt
     2941 ttggttccag agcagataaa tccgttctac aatcaactga ataaaatcct ttatccttag
     3001 tggctaatgg atgtttgata gggttatcgg catatttgcg atatctttct ccatctttac
     3061 taatagcctc ttcgtggaat tgatgacttg ttagtaattc agttagataa tgagatgcca
     3121 aagtccgtag aaactttgtt ttgaatcctc tgatatctcc ccaatcgagc agttctgctt
     3181 ttgcttgtcc gaattctttt aagcattgtt ttctgctttt cgtcttgtta acttggcaaa
     3241 taaaatgatg tgcatcgctc aaccttaatt ctttcgtgaa tactctataa aaggcgtttc
     3301 taatagagaa gtcatcatca ctaatcattc gccattcgtc ggcattaacc atttgtttaa
     3361 ttaaccaata gtgagcagtt gtagtataag gagcatcaac atttaaacca tcgacaaatc
     3421 tactcctatt agtaattttc cctaattcgt gaagataagc attcctattt tcgctatcgt
     3481 tagcggatgg ttcttgtgga taataagacc aatctaacct atcattttta cgactaaaag
     3541 tattgagatg gtcttctttg aattttcttg tatcttcatt taattcatcc attgaaatat
     3601 tccaatcata ggcaacatca gaacggaaca catatcttga taagacctca gcagatacaa
     3661 ctatatacgt ttgcatatta aggtcttcaa accccgtata tttctttgaa ccttgtcctt
     3721 tcttacggac gttatttagg tagtagtgca tcttgtccgt attaagccac atctcgttaa
     3781 atttctttgt ttgaaggggt tgtgtctcat atcgttctaa aaactccaaa caacgcctat
     3841 acagccactc cagcttatga tagtaggttc caactccaat tcctaaaata tcacaagtac
     3901 gactgactga aacccttcca acaaccatct tagcgaacat tggaagaatg gtgttctttt
     3961 gttggtgata agtagtggtt tcttctcttt ttggtagaac attagtgaac ttcttacacg
     4021 ccttacattg atagcgttga gatttgcccc tacttttccc ccttttgtaa aattgcttcg
     4081 gctcattgaa tggggttgat tcttcttccg agcagccttc tttatgaaat tgataatctg
     4141 gctcaacatc ttggatgcta ttgatttcta taaggcgttt aatttcttca acaacagacc
     4201 aattagataa aggggttact gtacagtttt gcgatacacc tctgccaata ggattgtcat
     4261 tgcagtacat acctttatga cctttatctt tggaactacc agtcatttta tatcgacttg
     4321 gtttcccttt tacatctttg aatttgtgct gttctttccc aaaattacaa caaaacggat
     4381 tgatgcagta attatattga atgggatatt cagaaccgtt ccatttaaaa gacgttggca
     4441 agaacaacat tttgttatac cttgttgcaa atcgttttgg agttaataaa tcatattgtt
     4501 tagccctatc ttttaattct tcgtttgaaa tcggaatttt aacatctaca atttcatctt
     4561 ccttcgtggc taatctttta agcttggtca ttgtctatca acccactcta tctttgatac
     4621 ccattttttc aacaatccgc ttattacttc gcttgttggc aatccattca ataaacttct
     4681 tatcgtctaa aatgttcaat aagaattcat ccactgcttc gctttcagta cgctctgcat
     4741 attcagcgta ttgcttaact atcaccctta cttgctctga tatttcccaa tccacagact
     4801 tagcgttctt attttttggc tcaagaaatt gcataggaaa atgcacccct ttttgcttag
     4861 taaattgtaa ccattttaac acaccaattt gcattcctac aataattatt tcacgttcgc
     4921 atttttatat acattttaac ttacattttg tggtgtaaat taacaacaaa cgttgattta
     4981 acaataaaaa acgagtatca aattcagttg caatttgata ctcatttagg tttacatttt
     5041 cactttttgt actccaaaac tgaactactt aatatgcaag acgttgtctt ttttttgtat
     5101 attcaatttt gaaaacattt ccgcaaagtt cagacggacc tcgatgtgtt gatgaataaa
     5161 atttattagt aataacttct ataaggttat caatccctat attacttttt gcacttccat
     5221 gataaagagg gaacagagaa caattctgaa atcttatgct ttcctcttgt tcgagttcca
     5281 atgcttctaa tgatttaccg gacatatatt tctctaaaag gtcatcgttt ccctctatta
     5341 ccgtatccca ttgttcagat tcggtaaagt tcgtcacaca catattagga tacagttcta
     5401 ccttctgttt gattacaatt tcggcagaaa gtttctcttt aatatcctga taaaccgttg
     5461 ataaatcaat tccattttgg tcaatcttat tgataaaaaa gattgtggga atccccattt
     5521 tcctaagtgc atgaaataat atacgagttt gtgcttgtac gccatctttt gcagaaatca
     5581 gtagaattgc cccatctaaa actgataatg aacgatatac ttctgctaag aaatccatat
     5641 gtcctggcgt gtctatgatg ttcaccttcg tattttccca ctgaaaagag gttattcctg
     5701 tctgaattgt aattcctctc tgacgttcta aaagcgtatt atccgtcctc gttgtacctt
     5761 tgtccacgct tcctaattct gtaatcgctc cactgttata taataagctt tctgttaagg
     5821 tagtttttcc tgcatcaaca tgagctaaaa ctccaatatt aataattttc atgtgatttt
     5881 cctccattca aaaacccaaa agggcataaa aatcccagtg ataaatactt ttatcactgg
     5941 gatttttatg cataaccata ggtatacaaa gcatacagat attctctgga tactttagaa
     6001 tcacatgata aaggtattct taaactgggt acaaaaaact aagccctcct aaaaaaggac
     6061 atctaattat ttgttcccgc tatcaaattg acagtttatt taagaatacc ttgccgcata
     6121 tttattaact ccttttaaat agtcacttaa ataatagcac gtaagagcat atttgtaaag
     6181 gaatctccaa ttttttatca aaaagagtac atgattacaa agtatctgta atcatgtacc
     6241 aatatttgtt attttacaat cttccaatta tcttgttttt ctaatatcaa ctcaaattgc
     6301 gagatttggg ttgcctttgt ttcttgatct aaatatttca cagcgacatt cacgattact
     6361 tggttatctt ttctggtaaa gataggattc accaattcct cgaaaacata ttccttgtta
     6421 atcataggta acacatgatt acttacatag taagtcaatt ctttttctgt ggcggtagga
     6481 tagagctgga agaacgtttc taaaaagcta gtgatttcgt ccatcgtttc tgtatctact
     6541 gtatggtcac tttctagctg tttgggttgg tagtccgatt tttgaggttt cttactcatt
     6601 gtcggatttt tgataataac catattatct gattcatcta tatggacaac cacatgaaag
     6661 ctagacgata tagtttcctt gtctttgtct tcggtaataa cttgttcaac agaaaacagt
     6721 acctcaaagg tgttctcatt cacttgagaa acctgccaca cctgtatatc gttcacacta
     6781 gaactagtag gaatatcctt acgaatcatt tcttcattta atacctgcaa ttcttccgtc
     6841 aaataatggg tcaatttttc atttcgttta tcaatggctt cttgggattg ttgccacgaa
     6901 aagtagtctt tcgcaaagga tttcacaaaa ctttctactt ggttggtatc gacaatctgc
     6961 tgtttaatga tttctgtttc acggacagta tgagtgtcaa tggcagtaaa atttttatag
     7021 attccaaatc cgacactacc aatcaataaa acccataaaa caaaggttaa tttcttatgc
     7081 gtgccaactt taatcatcgg aacttttctt tgcttgctcg gttttttaga tttctttggt
     7141 ttctccttct tttcttttcg ctcgattttt ataatcattt gattcattcc tttcttagtt
     7201 atgaataaca cgtctagccc caatcagatg gtcttgccaa taacgactgc ttaagtcgcc
     7261 ataaccgatt gggtcgcctg catgataaaa gcgatttccc tctaaatata tcgccacatg
     7321 agtcacatat gtgcctgcat tataggttga atgaaagaag ataaggtcgc ctgcttgggc
     7381 ttcttccata gagatttctt gcgtagcgtc atactgatct tgggcgacac gagggagaga
     7441 aatgccagct ttgtcataca cccattgtat taagccacta caatcaaaag aggtggtagg
     7501 cgacgcccct ccaaagacat agggaaaccc ttcgtatttc aaggcttcgt ccataatgac
     7561 ttgcacggtt tcatcatcaa attctgttgg ggaagtgtcg gttaggtatt gtgaaaccaa
     7621 ctgaacataa aactgatttc catagttata tcgccaacca ccattcacag gtatggctat
     7681 aggattggga tagtctgctt tttgaccacc cgacttctct ttagaaaact gttcggctaa
     7741 ttcataggta tattttttcc catgactcct cacataattt aaaaagccac cgccataatt
     7801 ataagattgg ataacagaat ctatatccac tccttgttgt tctgcactgg ttaataattc
     7861 actgaaatat ttaacccctt gtttgatgga ctcttcggta ctcaaactgt tgggcggtaa
     7921 acccaaagat tcggaacttt gcataacatc ttctgctgta ccgcccgatt ccacttgcat
     7981 aatggctaag atataagaaa catagtcttc aatcccatat tccttaccat atttttcaat
     8041 caacggacgg tgtgccaaca cttctggtga aacggtcaca ccgccttgtg gaatagaaga
     8101 atccttgttg ttttcgccac tgtccgtatc atctgcaaag aaaatgacta ggaataataa
     8161 gagaaaaaag aagagagaga agaccacact acaaatcact gttattttta gcttcatgtt
     8221 tgttgtcctc gcttatccgt tcttttattt agcttcctct gattgaattg cttcggtggt
     8281 gtcttttgtt tttgctcttt tttcaatggt cgctgatgat tttcacgttt agtgttttgt
     8341 gacaaaacag gcgtgacggt ttgctgacgc ttgcgaacat gctgctggtt attctgaaca
     8401 ggagatgatg ggttttcttt tcgtcttttt tcttttgctt tatccagctc caaacgcttt
     8461 tgagcaatgt tctttcgttt ttgctcctgc tggttcatac gctgttgttt gcgttgagct
     8521 ttggtatctg ccatagattg tttaaagtct gttacattat gagtgacatt ctcttttcct
     8581 tgatgaaggg catatttgac atgggttggt gtcgcctgta tctgttgttt tgcctgcttt
     8641 cttttattga ctagacgatt tttcgtgtcc aatacatggg ctgtcttttc acctaatcgt
     8701 tgcccgatac cgccttgttg ctttttcaca ttggaaagtg ctgtttcttt ctttggtgta
     8761 gtccgttcat tttgacgagt atggttagct ttattggttt gattctgctt tgttgtcatt
     8821 gtcttttgct tcgttgaccc tgcttttcca cgaaaaatct tactcatact gcgttgaaat
     8881 cttctggttt gtcgattcat catttgacga ggtctgcgaa acacttgtct gcccatgttt
     8941 tgagagtcgt tgctttgtaa agtgaatagg ctcattaaat cgcccagttt aaaataaatc
     9001 cctgcaaagg tcacaatttg taaaaacata atcatgaaaa agggagaact tgctgacagc
     9061 gaataaagca tagtggaaat actaaaggca acggtaataa tcaaggtaat accagccctt
     9121 gtcataatca cattaaataa cttggtaatg gcatgcctgc ccatgccatc aaaactcggc
     9181 aacatactta ataggaaact gattggcaaa aacatagcga aaataataaa tagcacttgc
     9241 gaaaaaatca tgattcctgt tagaaggaaa acaaaaatcg agataccaat attgaataca
     9301 aacaaaaaga agactgttcc taatctggaa atcgtcttcg gtagggttaa ataggtattg
     9361 tcatgatctt caatttcttc aatcactaca ttttctctat cttcaccatt gtttgtatct
     9421 ggactggtag acaataaact ttccacacgt tcttccccta acgcttctac atcagtggag
     9481 ccatattgaa gcaatagcca tggttgcttt acttgaacag agaataagct gtcacgtatc
     9541 atgtccacac tgtctttccc ttgactatcc gtgttaggaa gaacaatttt tgtcccaata
     9601 ttcagactgg cggtactgac atctttggaa aaatcattga ttttcgcaat ataatcgggt
     9661 gcatacgcaa taaaggaagc cgatagaata aacaccacga caaagtttaa aacagctcgt
     9721 atcactttcg tcgtttctcg cttaaccaat cctgtatagg caacatagat tccaacaatg
     9781 agaataaaaa gaaggaggaa accgacataa aaccctgaac tggaaaagcc attcggtgta
     9841 atgcctgcaa tcgtctgcat gttttcgcca attgcttggg atgtttgact aatgaaatct
     9901 aaggcatagg cttctttcac caaataacct gtggcgttcg atagataaag agaaattgtc
     9961 caaataaaat tagtaatggc atacaatcca tacatcaccg attgtcctat gccatcactc
    10021 caattccatg gaagccagcc ccagctattg tccacataat aatcaagctg atattgattc
    10081 aaaccatact tggagtattc attg
//
LOCUS       DACDTH010000251         9684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.guided.093, whole genome shotgun sequence.
ACCESSION   DACDTH010000251 DACDTH010000000
VERSION     DACDTH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.guided.093"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            80..862
                     /locus_tag="IWK54_003117"
     CDS             80..862
                     /locus_tag="IWK54_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP9595133.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(988..1719)
                     /gene="erm(A)"
                     /locus_tag="IWK54_003118"
     CDS             complement(988..1719)
                     /gene="erm(A)"
                     /locus_tag="IWK54_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP9595134.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(1777..1864)
                     /locus_tag="IWK54_003119"
                     /pseudo
     CDS             complement(1777..1864)
                     /locus_tag="IWK54_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            2228..2890
                     /locus_tag="IWK54_003120"
     CDS             2228..2890
                     /locus_tag="IWK54_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9595135.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            3125..3268
                     /locus_tag="IWK54_003121"
                     /pseudo
     CDS             3125..3268
                     /locus_tag="IWK54_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            3418..3618
                     /locus_tag="IWK54_003122"
     CDS             3418..3618
                     /locus_tag="IWK54_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9595136.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(3872..5551)
                     /locus_tag="IWK54_003123"
     CDS             complement(3872..5551)
                     /locus_tag="IWK54_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9595137.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(5553..5765)
                     /locus_tag="IWK54_003124"
     CDS             complement(5553..5765)
                     /locus_tag="IWK54_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9595138.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            6158..7888
                     /gene="efrA"
                     /locus_tag="IWK54_003125"
     CDS             6158..7888
                     /gene="efrA"
                     /locus_tag="IWK54_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9595139.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            7885..9645
                     /gene="efrB"
                     /locus_tag="IWK54_003126"
     CDS             7885..9645
                     /gene="efrB"
                     /locus_tag="IWK54_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9595140.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
BASE COUNT         3020 a         1622 c         1961 g         3081 t
ORIGIN      
        1 gtatatgtcc aatctagggt aagtaaattg agtatcaata taaactttat atgaacataa
       61 tcagttacga ggtgaaatca tgagcaattt gattaacgga aaaataccaa atcaagcgat
      121 tcaaacatta aaaatcgtaa aagatttatt tggaagttca atagttggag tatatctatt
      181 tggttcagca gtaaatggtg gtttacgcat taacagcgat gtagatgttc tagtcgtcgt
      241 gaatcatagt ttacctcaat taactcgaaa aaaactaaca gaaagactaa tgactatatc
      301 aggaaagatt ggaaatacgg attctgttag accacttgaa gttacggtta taaataggag
      361 tgaagttgtc ccttggcaat atcctccaaa aagagaattt atatacggtg agtggctcag
      421 gggtgaattt gagaatggac aaattcagga accaagctat gatcctgatt tggctattgt
      481 tttagcacaa gcaagaaaga atagtatttc tctatttggt cctgattctt caagtatact
      541 tgtctccgta cctttgacag atattcgaag agcaattaag gattctttgc cagaactaat
      601 tgaggggata aaaggtgatg agcgtaatgt aattttaacc ctagctcgaa tgtggcaaac
      661 agtgactact ggtgaaatta cctcgaaaga tgtcgctgca gaatgggcta tacctctttt
      721 acctaaagag catgtaactt tactggatat agctagaaaa ggctatcggg gagagtgtga
      781 tgataagtgg gaaggactat attcaaaggt gaaagcactc gttaagtata tgaaaaattc
      841 tatagaaact tctctcaatt aggctaattt tattgcaata acaggtgctt acttttaaaa
      901 ctactgattt attgataaat attgaacaat ttttgggaag aataaagcgt cctcttgtga
      961 aattagagaa cgctttatta ctttaattta gtgaaacaat ttgtaactat tgaaaataga
     1021 aagaaattgt tccttcgata gtttattaat attagtgaca tttgcatgct tcaaagcctg
     1081 tcggaattgg tttttagtga aaagaacacg atattcacgg tttacccact tataaacaaa
     1141 agatcgatac tttttgtagt ccttctttga aatcaatggt tgatgtcgtt caagaacaat
     1201 caatacagag tctacacttg gcttaggatg aaaatatagt ggtggtactt ttttgagcat
     1261 ttttatatcc atctccacca ttaatagtaa acccaaagct cgttgcagat tttgcaatct
     1321 tttcgcaaat cccttctcaa cgataagata gctatattta gcctgacttt caaaggtaat
     1381 tcttttgaca atatccgtac tgatgttata aggaatatta ccatatatct tatagtttat
     1441 atgttttggg aaggaaaatt ttagaatatc cgtttgaatc acttttatat tctcagaggg
     1501 gtttaccgct tctttagtca cttgacataa gcctccatca atttctatag cagtaactga
     1561 tcgactcatt ttgactagct ctttggtaaa atgtcctttt cctgatccga tttctattac
     1621 gttgtcttgt ttactgatat tcgtgtgatt caatatttct tttacatgct ttttagaagt
     1681 aataaaattt tgcgtgtctt tagggttttt ctggttcatt ataaccttct ccttactggt
     1741 tataatgaac tgacattaag cgttcattat aaccaattaa ttttgatttg gttgataacg
     1801 aactttatta ataacaaata tagaaaaagt acccatactt ttatctcctt tccatttttt
     1861 tcattatgaa tgagataaag taatttctac tactgcgata ctggtgcaca taattgaaac
     1921 ctccttatgt aactgaatat gattgtatca tcttttcatt ttagaataaa cctttatatg
     1981 taattttgca cttgacactt cagatggacg ttttcttcaa aataatatgt tttgaaactt
     2041 gataagagaa gccactgtct ttactaaaaa gtaaaggctg cgataaagga cagtgacttt
     2101 aataaagttg cactgttgta ttgtctttgg ataaaattgt ctaaagggaa gttttatccc
     2161 caacgaaaaa acatctaaag tggtaagtaa tgaaagaatg tcgataaagt aggaatgggg
     2221 gatctttttg aatacaatag aatcacagtt tgataaggtt gcagaagatt acgatttcgt
     2281 gaatgagctt ttgaatgatt attcattctt tgtgtctaat atgtctccaa agaaaggcag
     2341 agcattagat atcggatgtg gctcgggttt gttagtggag aaattagcaa gttattatga
     2401 tgaagtggta gggattgata tttctaatca aatgctcgat cttgccaaat ctaaacgtca
     2461 actaacaaat acggtctatc tgaatatgaa tgcagaacaa cttaatttta atgagaagtt
     2521 tgattttatt gtaagccgaa caacctttca tcatttggat gatatagcca gcgtaataca
     2581 gcaaatgaag gaactgttga atgaggaagg aagaatagtt attcttgata atgtatctga
     2641 agtcgagaca ccgcctacct atgtttataa attaggggca atccaagagt ttttaccgca
     2701 ttgtttcaaa tttggaataa aaaatgcgat tagaatttat aatcacaaca catctaaatc
     2761 atggcttgag catttagctt cagataagta cctatctgaa caaaattatt acgatttata
     2821 tgaaaagttg ttgcctggat gccaattcca taaaatgggt tgggcaatgg gggttgtctg
     2881 gacaaaataa cttaaagctg tggctttgaa aagttgaatt tgtgattgtt ttcagagcta
     2941 tcgggtgctt tagtataaat aacaatgaat aaaaaatcag cctgtatctt atgttactat
     3001 taagtaattt aagatacagg ttttactatt tttgaaataa gtaccttcca cttcaaatac
     3061 atgtattcaa agtgaaagta ttgatttaaa tgattctttt gtaatttact cttgataata
     3121 tgaaattcca tcgcagcagg atatgaattt taccaaaagg atacaaaagt gtggagaaat
     3181 gatggggatc actgtactgg accacttgat catcggacgc aaacgatatt ttagtttgag
     3241 agaagaagga atgatggaag agaagtaagt tgctttcttg caaaaaaaat gaagcaagtg
     3301 tacaattata ttgtaatttt gtttgggaat agaagttgaa acatctgttg cacctctatc
     3361 gtacacgagc aatgttacaa tttaaattag tgcttttggc acgaggagga tattattatg
     3421 gaaaacggaa cagtaaaatg gttcaacgct gaaaaaggat ttggatttat ctcacgtgaa
     3481 gatggaagcg acgtattcgt tcacttctca gctatccaag gtgacggatt caaaacatta
     3541 gaagaaggtc aagcagtgac tttcgacgtt gaagaatcag accgcggccc acaagctgca
     3601 aacgttgtaa aagcttaatt ttatctgtaa atcaaattga tggtagacat caatttttac
     3661 gagagaacaa agcgagtaac aaaaatacct gccacaagag acaatttctc ttgtggcagg
     3721 tatttttttg tatgaaaaag taaataaatt taagaggcta tgaaagagcc accttgtatc
     3781 aaggaataag aacaaacaaa aatggcttct catgtttttc atttgttttc tcttatattc
     3841 aggacaaggt gctgatttca taacctctta tttattcttc atctggtgtc attaccattg
     3901 gcaaaatcat tggatgtctt tctgtacgtt cgaataagaa aggctgaagc gcgccagtca
     3961 tcgcttcttt caatttatat tctgaacagt caggtccatt caaacattct cttaaagcgt
     4021 ggaataagat tcgctgagct tcattgatca tttctcctga ttctcgcatg tagacaaatc
     4081 cacgagagag aatatctgga ccagccaaga tttctttttt cttgatatcc actgtcgcga
     4141 cagctaatac aagaccttct tctgaaagca ggtgacggtc acgtaaaacg acattaccaa
     4201 tatcaccgat cccgctacca tcgatataca catctccagc cacaaaatgc ccagcaggac
     4261 gcgcagaatc gctagttaac gctaaaacat cgccattttc catgatgaag cagttttctg
     4321 caggaacacc tacatcttgt gctagagaag catgtacttt caacatgcgg aattctccgt
     4381 ggacaggaat aaaatatttt ggcttcatca aacgaagcat taatttttgt tcttcttgtc
     4441 caccgtgacc ggaagtatgg atgttattga tttttccgtg aatcacttct gcaccagctt
     4501 cagacagcaa attgatcaaa tgattgacac ttgttgtatt accaggaata ggtgaacttg
     4561 aaaagatcac tgtatcgtct ggctggattt ggatttgacg gtgagtacca ttcgcgatgc
     4621 ggctcaacgc ggccatcgct tctccttgag aacctgtaca taggatcatc gtttcattgg
     4681 caggcaagcg attgatttcg tgtgcatcta caaatgtgcc gtctggaact ttgatatatc
     4741 ctaaacgttg accgttgacg atggcatttt ccatgcttcg tccgaaaaca gcgattttac
     4801 gaccagttgc cacagcggca tctgctgctt gctgcaaacg gaagatgtta gaagcaaagc
     4861 tggcaaaaat gatacgtcct tcgatttttg aaaagatctt caaaatagaa tgcccaatcg
     4921 ttttttctga tttagtaaat gttggaatct cagcatttgt actatcagat aaaaggcaca
     4981 agactccttc ttccccgata cgagccattt tatgcaagtt tgctggttcg ccaacaggag
     5041 tgaaatcgaa tttgaagtct ccagtcgcta cgatatttcc agaaggtgtt ttcaccacga
     5101 tccctaatgc atctgggata ctatgggtcg ttctgaaaaa gctgacagaa gtctttctaa
     5161 aacggatgat cgtatcttca ttgatctcat ggagctctgc atctcgaagc aagccatgct
     5221 catctaattt gtttgtgatc aacgctaatg cgagaggacc agcatagata ggaatatttg
     5281 cttgtttcag caagtagggt attccgccga tgtggtcttc gtgcccgtga gtaatcacca
     5341 aggcttttac tttgtgtaag ttttgtatga tgtagctgta gtcgggaatg acatagtcaa
     5401 ttccaagtaa atcgtcttcc gggaatttga tcccggcatc aatcaaaatg atttcatctt
     5461 ggaattgtac cccatacgta tttttcccga tttcacctaa tccaccaata gcaaagacgc
     5521 ctgtctcgtt atttttgatg gaaagtttca tattaaaact ccactaatga gaaatcagca
     5581 ttttcttttt cgtactctag atgacttcct tctagaagtt gaacgaattc aatattgtat
     5641 ggggtatttt cttccacacg ttggcgagcg tctacatcgc tttttgcctc aatgtaaaga
     5701 gattttgttt cttcccgttt agggttgcgt acttttgttt cttgataata aactttatag
     5761 atcattgttt tgtctccttt atctgcgtaa agcagtgatt tagctagttg atttttttag
     5821 acaacacaaa acgaggggac aagccacctg gtttttcata tgctttgact agaatactgg
     5881 attcgagtca aagagacaag cctcgttttt gcttatctgt tcggatattt tttcaaccgt
     5941 accttctgta taaaaaatat acaacaatta attgttattc tagcataaaa cagcaaataa
     6001 gtatagaaat caactagatt gaaagaaaaa atcaatgttt atcatcccgc tataatcata
     6061 atgacttttt tatgagagaa gtgttctatt aataggctcg ttgtgctatc atttttagta
     6121 atgaaaatgt aacaaaagat taaaaggaga tgagaaagtg aaactaatgt ggcattatac
     6181 catgcgttat aagaaattct tgtttttcaa tttcatatgc gtatttggtt ttattttgat
     6241 tgaattagga cttcctacga ttttagcacg tatgatcgat gtaggaatcc gcaatgatga
     6301 ttttgattat gtgaagcaac aagggatatt tatgatcgtt attactgtaa tcggtattat
     6361 tttgaatatc ttgttaggtt attttggttc gaggattacg actaatatcg tagcagatat
     6421 tcgagatgat ttgttcgaaa agatccaaag ttattcgcat caggaatacg aaaatctagg
     6481 cgtatcttca ttgatcactc gtacaacaaa cgatgcttat cagatcatgt tgttcctgca
     6541 aaatatttta cgtatcggtt ttatggcacc aatgatgttc attgtcagtt tgtacatggt
     6601 catgcgtaca agtgtgacac tcagtttgta tgtagtaggt gcattacctt tactattatt
     6661 ggcagtcgta gcgattgcta aggtgtctga accgttatct aaaaagcaac aaaaaaactt
     6721 agataaaatc aacagtatct tgcgggaaaa tctttcagga ttgcgtgtga ttcgtgcatt
     6781 cgtcaatgaa aagtttgaag aaaaacgttt tgctgtagtc aatgagaatt atgcttctag
     6841 ttcaaaaagc ttattccgtt tgatggctgt tgcacaaccg ggtttttatt tcttatttaa
     6901 tatcgtaatg gtattgatca tctggagcgg ggcagtgcaa atcgatgcag gcgagcttcg
     6961 tgtaggagat ttgattgcgt ttatcgagta tatattccat gccttgttct cattcatgtt
     7021 atttgctagt gtgttcatga tgtatccaag agcagctgtt tcagctggac gtatccaaga
     7081 agccctagat gccaaacctt cgattgtaga agatccaaat ggtatatctg atacaaaaac
     7141 taaagggtat atcgaatttc aaaatgtaac atttgcttat ccaggccatt cgcaagagcc
     7201 agtgatacga aatgtcagtt ttacagcctc tccaggagag accgttgctt ttattggaag
     7261 taccggtagc ggaaaatcta cgttgatcca attgatccct cgcttttatg atgtgagtga
     7321 aggaaagatc ttgatcgatg gagtagacgt gagagattat cggctaagca agttgcgaga
     7381 taagattggg tttattccgc aaaaagcgct gctatttact ggaacgattg cagataattt
     7441 gcgatacggg aaagaagatg ctacgcaaga agaaatggaa cgtgcagctg agattgctca
     7501 agcttcagaa ttcatttctc gaaaaccaga tggatttaat gaacatttat cagaaggtgg
     7561 ttccaatttt tcaggtggac aaaaacaacg attggcaata gccagagcca tcatacgccg
     7621 tccggaaatc tatatttttg atgacagctt ttctgcatta gattatcaaa cggatgcgaa
     7681 gttacgtgct cgtctgaaaa aagaaacgac agaatctact gtattgatcg tagcacaacg
     7741 tgtgggaacg atcatgcatg cagataagat catcgtatta aatgaaggag aagtcgtcgg
     7801 tatggggacg catcgggagt tgttggaaaa atgcccgatt tattatgata ttgccgcttc
     7861 tcaattatca aaggaggaat tagcatgaaa aaaacctttt cttccttaaa acgtttagct
     7921 cgatatatca gaccgtatcg tgagactttt cttttagtga tcctatttac agttctgaca
     7981 gtggctttca acgtggcttt gccatatgtt acaggacttc ctactacaga aatcagccgt
     8041 aatattgcca atggcgaatc aatcaatttt tcttatatat tcaaatgtct catttggatc
     8101 acagttgttg gaatagggta ttgtatttcg caattacttt ctggtgtttt gatgacaaat
     8161 gtcgttcaaa gtgccatgca tgatttgaga agagacatag aagaaaaaat caatcgtctt
     8221 ccggtttcat atttcgataa aaaccagcaa ggaaatattt tgtctcgtgt gacgaatgat
     8281 gtagatgcag tcagcggtgc tttacaacaa gcttttattg gtatcgtaaa tgcgatattg
     8341 ggaattacgt tggctgcagc aatgatgttt tatatccagc cgatgatggc gctgatctct
     8401 atgatcatga ttccaacatc gatctggatc tcaaaacgag tgatcaatgc ttcgcagaaa
     8461 tattttcaaa gcatgcagaa ttctcttggt gagctaaacg gctacgtcca agaaaatatg
     8521 acaggtttta gtgtattgaa agtatatgga cgcgaaaaag aaacattgga aggattcaaa
     8581 aaagttaatc acagcttgaa atactttggg tttcgtgcag cattcatttc tggcttgatg
     8641 atgcctctag ttcaattaac tgcctatgct acttatattg gtatggctgt tctaggaagc
     8701 ttttatgtta ttactggggc gatcgttgtc ggtcaactac aagcattcat ccaatatatt
     8761 tggcaaatca gtcagccaat ggggaacgtg acacaattgt cttcagtttt gcaaagtgca
     8821 tcagcggcaa cgaaacgtgt attcgagatt ttagatgagc cagaagaaaa agaaaacaaa
     8881 gtagatgctt cactgccaga aacgatttta ggagatgtga cattcgatca tgtcgatttt
     8941 gcatatgatc cgaagaaacc attaatccaa gatttgaatt ttgaagtgaa agctggtcaa
     9001 accgtagctg tggtcggtcc gaccggtgcc ggcaagacga cattgatcaa tctattgatg
     9061 cgtttttatg acgtagacaa aggagcaata aggattgatg gaatcgacac caaatcaatg
     9121 agtcgcagtg atgtgcgttc gctatttggt atggtcttgc aagatgcatg gctttatcaa
     9181 ggaacgattg cagataatat ccgcttcgga aagcttgatg cgacagatta tgaagtagtt
     9241 gatgctgcga aaacagcaaa cgtagatcat tttatccgaa cgatgccaga tggatatgat
     9301 atggccatca attctgaggg agacaatgtg tctttaggac aaaaacagtt gctgacgatt
     9361 gcccgagcag tggtctccga tcctaaaatc ttgattttag atgaagcaac aagttcagta
     9421 gacacccgtt tagaagcgtt gattcaaaaa gcaatggatc gcgtgatgga gggacggaca
     9481 agtttcgtca tcgctcaccg tttatcgaca atccgtgatg cagatctgat tctagtgatg
     9541 gatcaaggtc agatcattga aaaaggaacg catgagagtt tattggcaca aggtggattt
     9601 tatgaaaaac tgtataacag ccaatttgct gaggaagcag aataagtcat ataagaataa
     9661 aacagtttaa aacttatttt ttgt
//
LOCUS       DACDTH010000252         6455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.guided.109, whole genome shotgun sequence.
ACCESSION   DACDTH010000252 DACDTH010000000
VERSION     DACDTH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.guided.109"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..685
                     /locus_tag="IWK54_003127"
     CDS             <1..685
                     /locus_tag="IWK54_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9595141.1"
                     /translation="AKIVLFFWNHFKAEHEAMLKQAQDEPAIDEIYHFDFLEAKELGL
                     KHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKRDVTTNYFILPHRG
                     NEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQKKLVTDNQAIAHYH
                     FYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKRFITNDSTIYEQYE
                     YDLSSVENK"
     gene            complement(835..1776)
                     /locus_tag="IWK54_003128"
     CDS             complement(835..1776)
                     /locus_tag="IWK54_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9595142.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1902..3023
                     /locus_tag="IWK54_003129"
     CDS             1902..3023
                     /locus_tag="IWK54_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9595143.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3281..4783
                     /locus_tag="IWK54_003130"
     CDS             3281..4783
                     /locus_tag="IWK54_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9595144.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4850..5377
                     /locus_tag="IWK54_003131"
     CDS             4850..5377
                     /locus_tag="IWK54_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9595145.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5588..>6455
                     /locus_tag="IWK54_003132"
     CDS             5588..>6455
                     /locus_tag="IWK54_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9595146.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTAT"
BASE COUNT         2126 a         1144 c         1369 g         1816 t
ORIGIN      
        1 tgcaaaaatt gtgttatttt tttggaatca cttcaaagca gaacacgagg cgatgctgaa
       61 gcaggcacaa gatgaaccgg caatcgatga gatttatcat tttgattttt tggaagcaaa
      121 agaacttggg ctgaaacata atagttcatt ttattcgaaa catatgaatt tttcttctag
      181 cgaaccaaag acggatctat tttttggtgc aacgaataat gggagaaaag aacgagcaga
      241 aagctataag caggaattta taaaaagaga tgttacgacc aactatttca ttcttccaca
      301 tcgagggaat gaacaacctg gatatcttac ttatgaagag tacctgaaaa aaacagggga
      361 atcgcgcggg attctggaac tgttgcggga gggtcagcaa ggagtgactt tgcggacatt
      421 tgaatccatg tattttcaaa agaaactagt gacggataat caagcgattg ctcattatca
      481 tttttatcgt cctgaaaata tttttttgct tcgagaacgt gatttagatg aattaccgga
      541 atttctgcat acaccattta agccaatcct atcagagcta ttggaatttt atgatgcaga
      601 aaattgggca aaacgattta tcactaacga ttccactatt tatgagcaat acgaatatga
      661 cctctcctct gttgaaaaca aataaaaaac ctacattttg aagagattat gtactgaaac
      721 aaacaagcac aattcttatg gcatcatgag agttgtgttt tctttttgat cttttattgg
      781 gcttaaaaaa agagaaccag ctgctgattc agctgattct catcagagag ccttttattc
      841 tgcgccaagg attccttctt ttgcatggac taattgggtc aaaaaggcac aatatggtgt
      901 agcaatatca tatttttgtc cttttcgcca aatggcacca ttgatgtagt cgatttcagt
      961 caaacgatgg tttttgatca ggtcttggta catagatggg taatgcaacc cgatgttgtc
     1021 aggatcataa gtagaagcaa tatgttggta cacttcttct tgatctagta tgattccttc
     1081 ttttgctgcg atagaagcaa attcagatac gattgttcga accatgctct ctgccgcttt
     1141 ttgcgcgcct agttcagcaa tattacaatc caaaatcgtg cataaaccat tcaatgttcc
     1201 atttacacaa gcttttcgcc aaatggaata acgaacgtta tggctataga tcggatttaa
     1261 atttgcttct tgaaagactt ctacaacttt ttttgtaaat gcttcgcctt ctggttccaa
     1321 gttctctagt tcgatttctc catcacctaa caatgtgact tttccgggac cagctagacc
     1381 agctgtccac atcgtgatcc caaataagat atttttcttt ggcacatatt tttccagtac
     1441 atcttcatga cctaatccat ttagtaaaca caaaacataa gtattgtcag taataatgga
     1501 ttggatcgaa caaaacatat catctaattg attggctttt gttaatgcta cgatcaagtc
     1561 cacgtgttcg ttgctttcga tgatttcttc tggtgaatag attggcagtt ttgcgaccac
     1621 ttctttaccg ttaaagtcag caatcaaccc atttgtacga atggcttcaa tgtgagcagg
     1681 ccaacgatcg atcaagagga cctcatttcc gctttgatgt agcatcaatc cgattcggct
     1741 gcccattgct ccggccccag ctattgcgat tttcatgttt tttctctcct ttattcaatg
     1801 agttcccttt tagtatatag aaaaatggaa gaaaatagca ttgttttgtt tgaaaaaaat
     1861 gaaaaaaagt gtcttactta agaagaaagt gagggataca gatggatatt ttggcggcaa
     1921 ttgattcatt taaaggaagt gcttcttcat ttgaactaaa tcaggcagtt ctaaatggct
     1981 gggaagatcg taaaggaaga aaaatcaatg tgccgattgc agatggcgga gaaaatactg
     2041 cagaagcaat ctattacgcg ctcaggggaa catggcgtac cattgatggg atggatctct
     2101 tattccgtca gcgaaaaatc agctatttat tgacaacttg gcaaggaaaa aacaccgcag
     2161 tcatcgaatc tgcacaagca ttggggttag atctacttga taaaccaacg gatcaaacta
     2221 tccgacttgc ttccagttat gccttaggag aacttattaa agatgcactg cttcaagaag
     2281 tagaacaaat tattgtcact ttaggaggaa gcgggtgtag cgatggtggt ctaggattac
     2341 tccaatcctt gggtgcttct ttagaagggt acacagaagg aaatccttta ttatctacta
     2401 aaaaacttca ttttgatcga agaaacgttc ttgtttctaa agtaaaattg attatcgctg
     2461 cagatgttac caatccatat acaggtcaac aaggagctgc ttggcaattt ggaagacaaa
     2521 aaggaggaat agatacgacg ctagctttct tagataaaca agcaggaaag attgctcagc
     2581 aaataaaaga tttctgggag gtggatttag atgatattcc aggaagtgga gcagcaggcg
     2641 gtattggtgg agcccttttt ttgttgggag cgaagatggt ttctggtttc gatttagtca
     2701 gccaaatcac tggattggaa aaaaagatcg aacaagcaga tgtcatcatt acaggagaag
     2761 ggcggatcga tcagcaaacg atccacggaa aagttccata cggtgtagca gtgcttgcaa
     2821 aaaaatatcg aaaaccaata atcgctattt gcggaagtag agaccaagag ctagaaggac
     2881 tttctgattt tctcccaatt gtcttaagta ttcatttggg tccaataggt ttagaagaag
     2941 caatggaaca caggcaaaca ttagaaaaaa caaaaatctt aggacagaca cttagcagat
     3001 ttttcaccat cctcccgaag tagttgacaa aatcattttc tttatgctat cgtaatttca
     3061 aatataggac aaagtgaggg atccgtgcat tgaaaaatta agtcgattga tgaaagctgc
     3121 attcagactg taccttgtgg agacaaggaa gcagtgtgca gattttcggg attggcttgg
     3181 ttttttcgat gtatgggtct ttttgtgtgt atccaaccct tgcatcctct ttagccagtt
     3241 ctgaaaatag gactggtctt tttttacgag gtgatggaaa atgtctaaaa tcgaaataaa
     3301 aaatctgaca ttcggctacg acagccaagg cacattatta tttgaacaag caaatctaaa
     3361 ttttgacaca caatggaaac taggacttat cggacgaaac ggtcgaggaa agacaacttt
     3421 actgaatatt ctacaaaaca aactacctta tcaagggcaa gtaatccatc agcaagaatt
     3481 tgcctatttc ccgcaacaga caaaagataa agaacgttta acctattacg tgttaaatga
     3541 tattacggat tttgagatat gggaaatcga aagagagctc caattgatgc aaacagatcc
     3601 tgaaatctta tggagagaat tcagcacact atcgggggga gagaagacaa aagtcctact
     3661 ggcactttta tttgtggatg acactcattt cccgttaatc gatgaaccaa cgaatcattt
     3721 ggatatctct ggtagaaaac aagtagcggc ttatttgaaa aagaaaaaac aaggcttcat
     3781 cgtggtcagc catgaccggg gatttatcga tgaagtagtg gaccatgttt tagcaatcga
     3841 aaaaagtcaa ctggaacttt atcaagggaa tttctctatc tatgaagaac agaaaaaact
     3901 tcgtgatgaa tttgaaatgg ctcaaaatga aaaattgaaa aaagaagtca gtaggctaaa
     3961 gaaaacagca gctgaaaaag ccgaatggtc tcgttcccga gaaggagata aaacaaagaa
     4021 acaagtcgga ttcatcgata ctgaatctag acgagtgaat aaaggagcag tgggtgctga
     4081 tgctgcacgg acgatgaaac gatccaaagc aatcgtgaat cggatggaga cccagatcag
     4141 cgagaaagaa aaactattaa aagatatcga atatatcgat tctttgacga tgaatagcca
     4201 agcgtctcac cataagcgac ttttaagcgt agaagatctt caattagggt atgaaaatct
     4261 gttattcgag ccaattcatt ttacaatcga gcctcatcag cgggtggcga tttcaggtcc
     4321 taacggtgca ggaaagtcat ccattatcca ttatcttctg ggggcattca acggcaaggt
     4381 tataggagaa aaataccagc caaaacatct gagcattagt tatgcaagcc aaaattatga
     4441 agacaatcga ggaacgttgg cggaatttgc agagaaaaac caagtagact accaagcatt
     4501 tttgaacaac ctccgaaagc ttgggatgga aagagatgtt tttcataaca agatcgagca
     4561 gatgagtatg ggccaacgga aaaaagtgga attggctaaa tctttatcac agccagctga
     4621 actatataca tgggatgaac cattgaatta tttggatgtc ttcaatcaag aacaattaga
     4681 acaactgatc ttgaacgtga aacctgccat gttactagtg gaacatgatc aaaccttcct
     4741 ggataaagta tctactgaga ttatttctct tgagagaatc taacccttgt aatctcaaga
     4801 acaacaaggt atggttaagg ggaactttgc caacgaagga gagaatcaca tgctttatgt
     4861 aacgagacac ggagaaacaa cttggaacgc acaaggatta gtttgcggac gggcagatgt
     4921 gccgctgact gaaaaaggac agatgcaggc acagaaacta gcagaaaagg tagtagattt
     4981 gcctgttcca atcacgaaaa tcatccattc ccctcttcaa cgagcaagag atacagcaca
     5041 agcagttgct gatcgtctca gcttgccgct gaccgttgat gaacgattgg tagaaatgga
     5101 ttttggggat tatgatggac tgccaagcaa agatgagaac tttcaaaaag cacgtttagc
     5161 gttcgctgta cgattcccaa atggggagtc agtcttagat gtgtatgcac ggatcgttcc
     5221 tttattgaaa gaatgcatcg aagacgaaga aaatgtctat ctgttagttt gccataatgc
     5281 cttgataaga gtgatcaatg cttattttca tccgatgcct aatgaggggt tctttacatt
     5341 catggtagat aatacagaat tggtttctta tgagtaatta tgtttttgtg ttatttaaat
     5401 aaaatatttc aaggcactgc ttttgcttag cagtgccttt tttaggttat gacaattctt
     5461 ttgataatat aaactcaacc tttagaaatg aaatttaatt attttaataa aagtattgaa
     5521 attttgtttc tttagatata tactctagtc atagaaatga agacggatac agaaaaagag
     5581 ggataatatg tttggctttt caatatttat gaacgattct ttgtcagaag agaagaaaca
     5641 gtatatccgc caaatggcat ccaatggctt tgtcggtatt ttcacttcca tgcatatacc
     5701 agaagatgat gtttcggcat atcaaaagcg attgatcgaa ctaggaagcg aagcgaaaaa
     5761 gaataaacta gaattgatgg tcgatatttc aggagaggca ttggaacgag cgggtttttc
     5821 attagaagat ctaacgcctt tgaaagcaat tggtgtaaca ggtttaagaa tggattatca
     5881 tatttccaat cagcagattg ccgactggtc acatcaattg aaaatcagtt tgaatgctag
     5941 cacgatcacg cctaaagata tcgatgaatt gaaagaagca gaagctgatt tctcacagat
     6001 tgaagcttgg cataattatt atccacgtcc cgaaacaggg ctggacaaag aatggtatca
     6061 gaaaaaaaat caatggctca aaattcaagg tctccttgtc caaggttttg taccaggaga
     6121 tacagagttg agaggcccac tatatcaagg attgccaact ttggaagaac atcgagggat
     6181 ccatcctctt gcaggcgctc tagatctatt agcaagtaac acggatattg tttacatcgg
     6241 agatgcaggt ctgtcagaaa acgttcagga gcaatttgct tctttccaaa aagaacagac
     6301 agtcttgctc catacggaac ctgtagatga agaattttac gaatatatat tagggaagca
     6361 tacgaatcgg caagacgatg caagagatgt catacgaagc gtagatgcac gttttcgaga
     6421 gattccgcct atcccagcaa gaaatacagc tacgc
//
LOCUS       DACDTH010000253         5986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.guided.114, whole genome shotgun sequence.
ACCESSION   DACDTH010000253 DACDTH010000000
VERSION     DACDTH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.guided.114"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(41..1027)
                     /locus_tag="IWK54_003133"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="IWK54_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR"
                     /locus_tag="IWK54_003134"
     CDS             1179..1874
                     /gene="vanR"
                     /locus_tag="IWK54_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9595147.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="IWK54_003135"
     CDS             1852..3006
                     /locus_tag="IWK54_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9595148.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="IWK54_003136"
     CDS             3221..4189
                     /locus_tag="IWK54_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9595149.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="IWK54_003137"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="IWK54_003137"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9595150.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5827
                     /locus_tag="IWK54_003138"
     CDS             5219..5827
                     /locus_tag="IWK54_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP9595151.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1811 a         1161 c         1348 g         1666 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcagtgc
     5581 cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg ggagccgatt
     5641 tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca atgaagcgca
     5701 aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat atagcctcga
     5761 atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg atttccccgt
     5821 taaataaact tttaaccgtt gcacggacaa actatataag ctaactcttt cggcaggaaa
     5881 cccgacgtat gtaactggtt cttagggaat ttatatatag tagatagtat tgaagatgta
     5941 aggcagaggg ttctgttgca aagttttaaa taaagaataa aatccc
//
LOCUS       DACDTH010000254         2736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.guided.157, whole genome shotgun sequence.
ACCESSION   DACDTH010000254 DACDTH010000000
VERSION     DACDTH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.guided.157"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            249..1727
                     /gene="msr"
                     /locus_tag="IWK54_003139"
     CDS             249..1727
                     /gene="msr"
                     /locus_tag="IWK54_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9595152.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1774..2220
                     /locus_tag="IWK54_003140"
     CDS             1774..2220
                     /locus_tag="IWK54_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9595153.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2419..>2736)
                     /locus_tag="IWK54_003141"
     CDS             complement(2419..>2736)
                     /locus_tag="IWK54_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9595154.1"
                     /translation="TDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLDKTTATGLL
                     FTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQQVREQMQQ
                     FFE"
BASE COUNT          875 a          460 c          570 g          831 t
ORIGIN      
        1 tcgaactttt gaataacaac taaataaatt caaaagacat gtataaacaa caatttcatg
       61 gatggagtat gtccattttt tcgaacgaca gtagaatagg tgaaagctat gacagatttg
      121 gtgatttagt catagttatt tcgttcagag taggaaaaga tgtgatgcag tttttttgca
      181 aacagatttt tctgctcttt tttagtatcg ataattgttg ttttacatga aataggagtg
      241 taaaacatat ggaaaattta gcagtaaata taacaaatct acaagtcagc tttggcaacc
      301 agctagaact atctattgat tctcttcgtg tctatcagca agatcgtata gggatcatag
      361 gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat agccggtgaa ctttttcccg
      421 atcatgggaa aatccaaaca gagatcacct tcaactacct gcctcaatta acctatctcg
      481 ctgaagcaaa ggacctaaat ttggaattag ccagtcattt ccagttaaga ctggaagaaa
      541 cttcagagcg gaaatggagc ggaggggaag aacgaaagat cgagttgata cgtcttcttt
      601 cttcttatga acaagggatg cttctagacg agccaacaac ccatctagat agaaaaagta
      661 ttgatcgact gattgaagag cttcgttatt attatggtac gctggttttt gttagtcatg
      721 atcgctattt tctagatgag ctagcatcga aaatctggga agtaaaagac ggagaaatcc
      781 gagagttttc ggggaattat agtgcctatc tcactcaaaa ggaattggag aaaaagactc
      841 agctacgaga agcagagtcg atcatgaaag agaaaaaacg attggaaaaa tcgatccaag
      901 aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag taaaaagaaa aagcaacaaa
      961 tcagactgga tcggttgtct tcctctaaac aaaaagacag tgtacaaaaa gccatccaaa
     1021 agaatgcgaa aacattagag agaagactcc aaaaaatagg agaaacaacc aagccgcagc
     1081 agatgaaaca aatccgtttt ccagtaccaa aatctcttga actccacagc cgctatccca
     1141 tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac attactggta aatggtgatt
     1201 ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga aaatggttca ggtaagacaa
     1261 ccttattaga acatatccgc aaacaaggag aaggaatcct tctctctccg aaagtaagct
     1321 ttcaagtata tcagcaaaag ggttatcaaa tgacatctga agaatccatc attcgttttg
     1381 tcatgagaca aacagagttt tcagaatcgc ttgtccgtag tttgctgaat cacttagggt
     1441 ttgctcagga aactttgacg aaaccgttat gtacattaag tgggggagaa gcgacccgtc
     1501 tgacgattgc tttgcttttt actaagccaa gtaatgtgtt gctgttagat gaaccgacta
     1561 attttattga tatggcaacg atcgaagctt tagagaagct aatgcaaata tatccgggaa
     1621 cgattttgtt tacttctcat gattcttact ttgtcgagcg tacggctgat gaagtttatg
     1681 aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa tttttaaatg tgctacgatg
     1741 attttcgtga caaatttaaa ggagtggtac agattgccta caaataaaaa agaaggaata
     1801 atttttacaa caatgatgtg ttttttgatg gtcttgggga tgagcgcgta taatttatgg
     1861 ctgcatcaag atcttcattt atcaagtttg ttgattggtc tagtgcctgg attcgtggtg
     1921 gctttcattt tggatgtctt catcatcggt gtgctggcga aaaaaattgc ctttaaacta
     1981 ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta tttcatgctt gatggtttta
     2041 ggaatggtga catgtatgtc tttattcggt gtgatcatgg aacaaggaat acctgatcaa
     2101 ttattctctg cttatatgaa tgcttggaaa atgaatgtga ttgccgcttt accgttgcag
     2161 ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag caattcaaag taatgaatga
     2221 tggaacactg ttatttgaaa aaaggccttg gcatgctttt tgtcatagtc tcttgaatgc
     2281 agttctagaa aaatagtgat ccgaacgata ggttttggcc tgtcgttcgg atcgcttttt
     2341 cttttccaac tgtattgctc ttttattgaa gataatttgc tcttcaatag atatttctag
     2401 aaaggttctg tttatccctt actcaaaaaa ctgctgcatt tgttctctta cttgctgaca
     2461 ccattggagt tcctgctgac aaagcataat tctgcgctgg atcacaacat aacgtccaaa
     2521 ttcttgtttc ttgtctagcg agaggtcatt caattttttt tgattttttt gcatgatttt
     2581 gttcagctgt tgttctctag tgaatagcag tccagtagct gttgttttat cgagcgtcga
     2641 aaaagcatag atttttgcta gaaattcatc tttttgcgta ggagcgggag tttcctcttt
     2701 gatccattcc tctacaagag cacgtccttc atcagt
//
LOCUS       DACDTH010000255         1997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.guided.176, whole genome shotgun sequence.
ACCESSION   DACDTH010000255 DACDTH010000000
VERSION     DACDTH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.guided.176"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..729)
                     /locus_tag="IWK54_003142"
     CDS             complement(<1..729)
                     /locus_tag="IWK54_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9595155.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLV"
     gene            1282..1830
                     /gene="aac(6')"
                     /locus_tag="IWK54_003143"
     CDS             1282..1830
                     /gene="aac(6')"
                     /locus_tag="IWK54_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9595156.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          650 a          351 c          395 g          601 t
ORIGIN      
        1 aactaaaaca gaaaccaatc cggtagtaac aatgatatcc gaaacgactt gttcaattgc
       61 atatgaggga cgatcgtcgg cactatcttt caatgcttgc gtaccataag tcaagagacg
      121 atcaatgcac atttttccga tttggattcc taatctatcc gtgtagccta gatcatcgcc
      181 acgagcagaa aaagtagatt cgacttcttt ggataaagca tcgccaatac ctgcccataa
      241 gtatttatca ggtgcttcgg caataattgc tgtatcaatg aaggtgtgta caggtggttt
      301 tgaaggaaag aagagtccgt ccattacgcc ttcttcgtta tagatgacac ataccgcagt
      361 gacgggcgaa cagttcgaac caatagtggg aaaggtgaag actggtttag ctagtttttc
      421 accaatgact tttacagtat cgcaagcacg gccaccaccg actgcaaaca ggacgtctgc
      481 tttttggaca ctttcatgct ggatcaactg ttcaatattt ccataagttg aattaccccc
      541 gtaccagaga ctggcgacaa cctctatttc ctgtgtcaaa gctactttta ttttgttttc
      601 tgcctttgaa agtgcagtct tccctccaat cagcactact tttgtcccat attctttgac
      661 gattccattt gctttttcat acgcatctgt tccaatggta taatttggta agaaaacact
      721 ttgactcata ttcatttcct tctttcttcg ttgaaacggg ttaaaatttt aaagagtgat
      781 cgatctgact gcggctgtag atggagtaga ggtgaaccta agacaggata agtgatgaat
      841 aaggtggcat gaacaccttg aaatcatcaa taagtatcct aaaaacgaac aagtacaaaa
      901 acattgaaaa cgacaataat gaatttttgt tcctcttagt atttctaccg attccctttg
      961 cttttttgta attatctaaa aattctaaaa attaagatta ttatatgaaa acaggtttta
     1021 tttgtcaatt cataattggt tttttttaaa gcaaaagata atctatatca tgatgaacaa
     1081 acagagtttt ttcctccaat tgatggcttt cctgatcttt cctattttga ataaagtgtt
     1141 tctgtataat ttttttattt tataagtaag ataaataatg aaagaataga gatactgatt
     1201 ggtagctaaa gatttttgag cgagaacatt tcgttttttc aaaaggactg ttagaataag
     1261 caagaacaag ggggaagacg tatgataatc agtgaatttg accgtaataa tccagtattg
     1321 aaagatcagc tttctgattt actgagactg acttggccgg aagaatatgg agacagctcg
     1381 gcagaagaag tagaagaaat gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa
     1441 gatgagttag taggatttat tggtgcaatc cctcaatacg gtatcacagg ttgggaattg
     1501 catccattag ttgtagaaag ctcccgacga aagaaccaaa taggtactcg attagtcaat
     1561 tacttagaaa aagaagtagc ttccagagga ggaatcacga tttatttagg tacggatgat
     1621 ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg agcatacatt tgataaagtg
     1681 gcttccatcc agaaccttcg tgaacatccg tatgaattct atgaaaaatt aggttataaa
     1741 atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac cggatatttg gatggcaaaa
     1801 acgattattc ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga agtaatcagc
     1861 ttcaagaaat aagaaggaat ttacgaaaat tacttcttga atttttgtga aattccaact
     1921 tatttccgaa gaagctactt ttggaacacc gtgtaccaga ggttgtgaaa aagtcgctta
     1981 gtttcgagta gtaagaa
//
LOCUS       DACDTH010000256         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.guided.190, whole genome shotgun sequence.
ACCESSION   DACDTH010000256 DACDTH010000000
VERSION     DACDTH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.guided.190"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IWK54_003144"
                     /pseudo
     CDS             262..373
                     /locus_tag="IWK54_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IWK54_003145"
     CDS             394..477
                     /locus_tag="IWK54_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9595157.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IWK54_003146"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IWK54_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9595158.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACDTH010000257         1382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.guided.195, whole genome shotgun sequence.
ACCESSION   DACDTH010000257 DACDTH010000000
VERSION     DACDTH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.guided.195"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1311
                     /locus_tag="IWK54_003147"
     CDS             <1..1311
                     /locus_tag="IWK54_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAP9595159.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DACDTH010000258         1351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.guided.199, whole genome shotgun sequence.
ACCESSION   DACDTH010000258 DACDTH010000000
VERSION     DACDTH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.guided.199"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            17..409
                     /locus_tag="IWK54_003148"
     CDS             17..409
                     /locus_tag="IWK54_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9595160.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            410..>1351
                     /gene="aac(6')-Ie"
                     /locus_tag="IWK54_003149"
     CDS             410..>1351
                     /gene="aac(6')-Ie"
                     /locus_tag="IWK54_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HAP9595161.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDY"
BASE COUNT          555 a          111 c          224 g          461 t
ORIGIN      
        1 gaagaatatt atcctattga aactattgtt tattgatgat aatatggttt ttgatattga
       61 taatttaaaa ggatttctta atgataccag ttcatttggg tttatagcta aagaaaataa
      121 taaaattata ggatttgcat attgctatac acttttaaga cctgatggaa aaacaatgtt
      181 ttatttacac tcaataggaa tgttacctaa ctatcaagac aaaggttatg gttcaaaatt
      241 attatctttt attaaggaat attctaaaga gattggttgt tctgaaatgt ttttaataac
      301 tgataaaggt aatcctagag cttgccatgt atatgaaaaa ttaggtggta aaaatgatta
      361 taaagatgaa atagtatatg tatatgatta tgaaaaaggt gataaataaa tgaatatagt
      421 tgaaaatgaa atatgtataa gaactttaat agatgatgat tttcctttga tgttaaaatg
      481 gttaactgat gaaagagtat tagaatttta tgatggtaga gataaaaaat atacattaga
      541 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
      601 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      661 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      721 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      781 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      841 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      901 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      961 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
     1021 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
     1081 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
     1141 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
     1201 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
     1261 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
     1321 ttttttaaga caaatgcacg gtttagatta t
//
LOCUS       DACDTH010000259         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.guided.200, whole genome shotgun sequence.
ACCESSION   DACDTH010000259 DACDTH010000000
VERSION     DACDTH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.guided.200"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IWK54_003150"
                     /pseudo
     CDS             46..297
                     /locus_tag="IWK54_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IWK54_003151"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IWK54_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9595162.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACDTH010000260         1194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20813_2015
            SAMN12639633-rid9882693.guided.214, whole genome shotgun sequence.
ACCESSION   DACDTH010000260 DACDTH010000000
VERSION     DACDTH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639633
            Sequence Read Archive: SRR10030736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742041.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742041.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,152
            CDSs (total)                      :: 3,086
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 32 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 17 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639633-rid9882693.guided.214"
                     /isolate="E20813_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="IWK54_003152"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="IWK54_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9595163.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
